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Listeria monocytogenesListeriosisListeriaMeningitis, ListeriaFood MicrobiologyHemolysin ProteinsBacterial ProteinsVirulencePeptide Termination FactorsCheeseHeat-Shock ProteinsBacterial ToxinsColony Count, MicrobialNisinMeat ProductsFood-Processing IndustryMacrophagesSpleenBacteriocinsGene Expression Regulation, BacterialFood PreservationFood ContaminationFoodborne DiseasesDNA, BacterialMice, Inbred C57BLMolecular Sequence DataMice, Inbred BALB CFood PreservativesMicrobial ViabilityImmunity, InnateVirulence FactorsCulture MediaSerotypingFood HandlingMilkPhagosomesGenes, BacterialImmunity, CellularLethal Dose 50Caco-2 CellsPhagocytosisBacterial AdhesionBenzalkonium CompoundsInterferon-gammaAntigens, BacterialEnvironmental MicrobiologyAnti-Bacterial AgentsCD8-Positive T-LymphocytesSigma FactorBacterial VaccinesImmunity, ActiveMacrophage ActivationBetaineLiverHemolysin FactorsAmpicillinBase SequenceHypersensitivity, DelayedPediococcusPhosphoinositide Phospholipase CMembrane ProteinsBacterial Typing TechniquesGene DeletionHot TemperatureRefrigerationMutationBacterial LoadCold TemperatureMice, KnockoutStainless SteelAmino Acid SequenceHemolysisTemperatureSequence Analysis, DNAImmunologic MemoryAlanine RacemaseAcidsMeatSpecies SpecificityRibotypingImmunization, PassiveCell WallCell LinePeritoneal CavityElectrophoresis, Gel, Pulsed-FieldHydrogen-Ion Concentration
Listeria monocytogenes: A species of gram-positive, rod-shaped bacteria widely distributed in nature. It has been isolated from sewage, soil, silage, and from feces of healthy animals and man. Infection with this bacterium leads to encephalitis, meningitis, endocarditis, and abortion.Listeriosis: Infections with bacteria of the genus LISTERIA.Listeria: A genus of bacteria which may be found in the feces of animals and man, on vegetation, and in silage. Its species are parasitic on cold-blooded and warm-blooded animals, including man.Meningitis, Listeria: Inflammation of the meninges caused by LISTERIA MONOCYTOGENES infection, usually occurring in individuals under the age of 3 years or over the age of 50 years. It may occur at any age in individuals with IMMUNOLOGIC DEFICIENCY SYNDROMES. Clinical manifestations include FEVER, altered mentation, HEADACHE, meningeal signs, focal neurologic signs, and SEIZURES. (From Medicine 1998 Sep;77(5):313-36)Food Microbiology: The presence of bacteria, viruses, and fungi in food and food products. This term is not restricted to pathogenic organisms: the presence of various non-pathogenic bacteria and fungi in cheeses and wines, for example, is included in this concept.Hemolysin Proteins: Proteins from BACTERIA and FUNGI that are soluble enough to be secreted to target ERYTHROCYTES and insert into the membrane to form beta-barrel pores. Biosynthesis may be regulated by HEMOLYSIN FACTORS.Bacterial Proteins: Proteins found in any species of bacterium.Virulence: The degree of pathogenicity within a group or species of microorganisms or viruses as indicated by case fatality rates and/or the ability of the organism to invade the tissues of the host. The pathogenic capacity of an organism is determined by its VIRULENCE FACTORS.Peptide Termination Factors: Proteins that are involved in the peptide chain termination reaction (PEPTIDE CHAIN TERMINATION, TRANSLATIONAL) on RIBOSOMES. They include codon-specific class-I release factors, which recognize stop signals (TERMINATOR CODON) in the MESSENGER RNA; and codon-nonspecific class-II release factors.Cheese: A nutritious food consisting primarily of the curd or the semisolid substance formed when milk coagulates.Heat-Shock Proteins: Proteins which are synthesized in eukaryotic organisms and bacteria in response to hyperthermia and other environmental stresses. They increase thermal tolerance and perform functions essential to cell survival under these conditions.Bacterial Toxins: Toxic substances formed in or elaborated by bacteria; they are usually proteins with high molecular weight and antigenicity; some are used as antibiotics and some to skin test for the presence of or susceptibility to certain diseases.Colony Count, Microbial: Enumeration by direct count of viable, isolated bacterial, archaeal, or fungal CELLS or SPORES capable of growth on solid CULTURE MEDIA. The method is used routinely by environmental microbiologists for quantifying organisms in AIR; FOOD; and WATER; by clinicians for measuring patients' microbial load; and in antimicrobial drug testing.Nisin: A 34-amino acid polypeptide antibiotic produced by Streptococcus lactis. It has been used as a food preservative in canned fruits and vegetables, and cheese.Meat Products: Articles of food which are derived by a process of manufacture from any portion of carcasses of any animal used for food (e.g., head cheese, sausage, scrapple).Food-Processing Industry: The productive enterprises concerned with food processing.Macrophages: The relatively long-lived phagocytic cell of mammalian tissues that are derived from blood MONOCYTES. Main types are PERITONEAL MACROPHAGES; ALVEOLAR MACROPHAGES; HISTIOCYTES; KUPFFER CELLS of the liver; and OSTEOCLASTS. They may further differentiate within chronic inflammatory lesions to EPITHELIOID CELLS or may fuse to form FOREIGN BODY GIANT CELLS or LANGHANS GIANT CELLS. (from The Dictionary of Cell Biology, Lackie and Dow, 3rd ed.)Spleen: An encapsulated lymphatic organ through which venous blood filters.Bacteriocins: Substances elaborated by specific strains of bacteria that are lethal against other strains of the same or related species. They are protein or lipopolysaccharide-protein complexes used in taxonomy studies of bacteria.Gene Expression Regulation, Bacterial: Any of the processes by which cytoplasmic or intercellular factors influence the differential control of gene action in bacteria.Food Preservation: Procedures or techniques used to keep food from spoiling.Food Contamination: The presence in food of harmful, unpalatable, or otherwise objectionable foreign substances, e.g. chemicals, microorganisms or diluents, before, during, or after processing or storage.Foodborne Diseases: Acute illnesses, usually affecting the GASTROINTESTINAL TRACT, brought on by consuming contaminated food or beverages. Most of these diseases are infectious, caused by a variety of bacteria, viruses, or parasites that can be foodborne. Sometimes the diseases are caused by harmful toxins from the microbes or other chemicals present in the food. Especially in the latter case, the condition is often called food poisoning.DNA, Bacterial: Deoxyribonucleic acid that makes up the genetic material of bacteria.Mice, Inbred C57BLMolecular Sequence Data: Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.Mice, Inbred BALB CFood Preservatives: Substances capable of inhibiting, retarding or arresting the process of fermentation, acidification or other deterioration of foods.Microbial Viability: Ability of a microbe to survive under given conditions. This can also be related to a colony's ability to replicate.Immunity, Innate: The capacity of a normal organism to remain unaffected by microorganisms and their toxins. It results from the presence of naturally occurring ANTI-INFECTIVE AGENTS, constitutional factors such as BODY TEMPERATURE and immediate acting immune cells such as NATURAL KILLER CELLS.Virulence Factors: Those components of an organism that determine its capacity to cause disease but are not required for its viability per se. Two classes have been characterized: TOXINS, BIOLOGICAL and surface adhesion molecules that effect the ability of the microorganism to invade and colonize a host. (From Davis et al., Microbiology, 4th ed. p486)Culture Media: Any liquid or solid preparation made specifically for the growth, storage, or transport of microorganisms or other types of cells. The variety of media that exist allow for the culturing of specific microorganisms and cell types, such as differential media, selective media, test media, and defined media. Solid media consist of liquid media that have been solidified with an agent such as AGAR or GELATIN.Serotyping: Process of determining and distinguishing species of bacteria or viruses based on antigens they share.Food Handling: Any aspect of the operations in the preparation, processing, transport, storage, packaging, wrapping, exposure for sale, service, or delivery of food.Milk: The white liquid secreted by the mammary glands. It contains proteins, sugar, lipids, vitamins, and minerals.Phagosomes: Membrane-bound cytoplasmic vesicles formed by invagination of phagocytized material. They fuse with lysosomes to form phagolysosomes in which the hydrolytic enzymes of the lysosome digest the phagocytized material.Genes, Bacterial: The functional hereditary units of BACTERIA.Immunity, Cellular: Manifestations of the immune response which are mediated by antigen-sensitized T-lymphocytes via lymphokines or direct cytotoxicity. This takes place in the absence of circulating antibody or where antibody plays a subordinate role.Lethal Dose 50: The dose amount of poisonous or toxic substance or dose of ionizing radiation required to kill 50% of the tested population.Caco-2 Cells: Human colonic ADENOCARCINOMA cells that are able to express differentiation features characteristic of mature intestinal cells, such as ENTEROCYTES. These cells are valuable in vitro tools for studies related to intestinal cell function and differentiation.Phagocytosis: The engulfing and degradation of microorganisms; other cells that are dead, dying, or pathogenic; and foreign particles by phagocytic cells (PHAGOCYTES).Bacterial Adhesion: Physicochemical property of fimbriated (FIMBRIAE, BACTERIAL) and non-fimbriated bacteria of attaching to cells, tissue, and nonbiological surfaces. It is a factor in bacterial colonization and pathogenicity.Benzalkonium Compounds: A mixture of alkylbenzyldimethylammonium compounds. It is a bactericidal quaternary ammonium detergent used topically in medicaments, deodorants, mouthwashes, as a surgical antiseptic, and as a as preservative and emulsifier in drugs and cosmetics.Interferon-gamma: The major interferon produced by mitogenically or antigenically stimulated LYMPHOCYTES. It is structurally different from TYPE I INTERFERON and its major activity is immunoregulation. It has been implicated in the expression of CLASS II HISTOCOMPATIBILITY ANTIGENS in cells that do not normally produce them, leading to AUTOIMMUNE DISEASES.Antigens, Bacterial: Substances elaborated by bacteria that have antigenic activity.Environmental Microbiology: The study of microorganisms living in a variety of environments (air, soil, water, etc.) and their pathogenic relationship to other organisms including man.Anti-Bacterial Agents: Substances that reduce the growth or reproduction of BACTERIA.CD8-Positive T-Lymphocytes: A critical subpopulation of regulatory T-lymphocytes involved in MHC Class I-restricted interactions. They include both cytotoxic T-lymphocytes (T-LYMPHOCYTES, CYTOTOXIC) and CD8+ suppressor T-lymphocytes.Sigma Factor: A protein which is a subunit of RNA polymerase. It effects initiation of specific RNA chains from DNA.Bacterial Vaccines: Suspensions of attenuated or killed bacteria administered for the prevention or treatment of infectious bacterial disease.Immunity, Active: Resistance to a disease agent resulting from the production of specific antibodies by the host, either after exposure to the disease or after vaccination.Macrophage Activation: The process of altering the morphology and functional activity of macrophages so that they become avidly phagocytic. It is initiated by lymphokines, such as the macrophage activation factor (MAF) and the macrophage migration-inhibitory factor (MMIF), immune complexes, C3b, and various peptides, polysaccharides, and immunologic adjuvants.Betaine: A naturally occurring compound that has been of interest for its role in osmoregulation. As a drug, betaine hydrochloride has been used as a source of hydrochloric acid in the treatment of hypochlorhydria. Betaine has also been used in the treatment of liver disorders, for hyperkalemia, for homocystinuria, and for gastrointestinal disturbances. (From Martindale, The Extra Pharmacopoeia, 30th ed, p1341)Liver: A large lobed glandular organ in the abdomen of vertebrates that is responsible for detoxification, metabolism, synthesis and storage of various substances.Hemolysin Factors: Plasmids controlling the synthesis of hemolysin by bacteria.Ampicillin: Semi-synthetic derivative of penicillin that functions as an orally active broad-spectrum antibiotic.Base Sequence: The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.Hypersensitivity, Delayed: An increased reactivity to specific antigens mediated not by antibodies but by cells.Pediococcus: A genus of gram-positive, facultatively anaerobic bacteria whose growth is dependent on the presence of a fermentable carbohydrate. No endospores are produced. Its organisms are found in fermenting plant products and are nonpathogenic to plants and animals, including humans.Phosphoinositide Phospholipase C: A type C phospholipase with specificity towards PHOSPHATIDYLINOSITOLS that contain INOSITOL 1,4,5-TRISPHOSPHATE. Many of the enzymes listed under this classification are involved in intracellular signaling.Membrane Proteins: Proteins which are found in membranes including cellular and intracellular membranes. They consist of two types, peripheral and integral proteins. They include most membrane-associated enzymes, antigenic proteins, transport proteins, and drug, hormone, and lectin receptors.Bacterial Typing Techniques: Procedures for identifying types and strains of bacteria. The most frequently employed typing systems are BACTERIOPHAGE TYPING and SEROTYPING as well as bacteriocin typing and biotyping.Gene Deletion: A genetic rearrangement through loss of segments of DNA or RNA, bringing sequences which are normally separated into close proximity. This deletion may be detected using cytogenetic techniques and can also be inferred from the phenotype, indicating a deletion at one specific locus.Hot Temperature: Presence of warmth or heat or a temperature notably higher than an accustomed norm.Refrigeration: The mechanical process of cooling.Mutation: Any detectable and heritable change in the genetic material that causes a change in the GENOTYPE and which is transmitted to daughter cells and to succeeding generations.Bacterial Load: Measurable quantity of bacteria in an object, organism, or organism compartment.Cold Temperature: An absence of warmth or heat or a temperature notably below an accustomed norm.Mice, Knockout: Strains of mice in which certain GENES of their GENOMES have been disrupted, or "knocked-out". To produce knockouts, using RECOMBINANT DNA technology, the normal DNA sequence of the gene being studied is altered to prevent synthesis of a normal gene product. Cloned cells in which this DNA alteration is successful are then injected into mouse EMBRYOS to produce chimeric mice. The chimeric mice are then bred to yield a strain in which all the cells of the mouse contain the disrupted gene. Knockout mice are used as EXPERIMENTAL ANIMAL MODELS for diseases (DISEASE MODELS, ANIMAL) and to clarify the functions of the genes.Stainless Steel: Stainless steel. A steel containing Ni, Cr, or both. It does not tarnish on exposure and is used in corrosive environments. (Grant & Hack's Chemical Dictionary, 5th ed)Amino Acid Sequence: The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.Hemolysis: The destruction of ERYTHROCYTES by many different causal agents such as antibodies, bacteria, chemicals, temperature, and changes in tonicity.Temperature: The property of objects that determines the direction of heat flow when they are placed in direct thermal contact. The temperature is the energy of microscopic motions (vibrational and translational) of the particles of atoms.Sequence Analysis, DNA: A multistage process that includes cloning, physical mapping, subcloning, determination of the DNA SEQUENCE, and information analysis.Immunologic Memory: The altered state of immunologic responsiveness resulting from initial contact with antigen, which enables the individual to produce antibodies more rapidly and in greater quantity in response to secondary antigenic stimulus.Alanine Racemase: A pyridoxal-phosphate protein that reversibly catalyzes the conversion of L-alanine to D-alanine. EC 5.1.1.1.Acids: Chemical compounds which yield hydrogen ions or protons when dissolved in water, whose hydrogen can be replaced by metals or basic radicals, or which react with bases to form salts and water (neutralization). An extension of the term includes substances dissolved in media other than water. (Grant & Hackh's Chemical Dictionary, 5th ed)Meat: The edible portions of any animal used for food including domestic mammals (the major ones being cattle, swine, and sheep) along with poultry, fish, shellfish, and game.Species Specificity: The restriction of a characteristic behavior, anatomical structure or physical system, such as immune response; metabolic response, or gene or gene variant to the members of one species. It refers to that property which differentiates one species from another but it is also used for phylogenetic levels higher or lower than the species.Ribotyping: RESTRICTION FRAGMENT LENGTH POLYMORPHISM analysis of rRNA genes that is used for differentiating between species or strains.Immunization, Passive: Transfer of immunity from immunized to non-immune host by administration of serum antibodies, or transplantation of lymphocytes (ADOPTIVE TRANSFER).Cell Wall: The outermost layer of a cell in most PLANTS; BACTERIA; FUNGI; and ALGAE. The cell wall is usually a rigid structure that lies external to the CELL MEMBRANE, and provides a protective barrier against physical or chemical agents.Cell Line: Established cell cultures that have the potential to propagate indefinitely.Peritoneal Cavity: The space enclosed by the peritoneum. It is divided into two portions, the greater sac and the lesser sac or omental bursa, which lies behind the STOMACH. The two sacs are connected by the foramen of Winslow, or epiploic foramen.Electrophoresis, Gel, Pulsed-Field: Gel electrophoresis in which the direction of the electric field is changed periodically. This technique is similar to other electrophoretic methods normally used to separate double-stranded DNA molecules ranging in size up to tens of thousands of base-pairs. However, by alternating the electric field direction one is able to separate DNA molecules up to several million base-pairs in length.Hydrogen-Ion Concentration: The normality of a solution with respect to HYDROGEN ions; H+. It is related to acidity measurements in most cases by pH = log 1/2[1/(H+)], where (H+) is the hydrogen ion concentration in gram equivalents per liter of solution. (McGraw-Hill Dictionary of Scientific and Technical Terms, 6th ed)

Phenotype of mice and macrophages deficient in both phagocyte oxidase and inducible nitric oxide synthase. (1/3136)

The two genetically established antimicrobial mechanisms of macrophages are production of reactive oxygen intermediates by phagocyte oxidase (phox) and reactive nitrogen intermediates by inducible nitric oxide synthase (NOS2). Mice doubly deficient in both enzymes (gp91(phox-/-)/NOS2(-/-)) formed massive abscesses containing commensal organisms, mostly enteric bacteria, even when reared under specific pathogen-free conditions with antibiotics. Neither parental strain showed such infections. Thus, phox and NOS2 appear to compensate for each other's deficiency in providing resistance to indigenous bacteria, and no other pathway does so fully. Macrophages from gp91(phox-/-)/NOS2(-/-) mice could not kill virulent Listeria. Their killing of S. typhimurium, E. coli, and attenuated Listeria was markedly diminished but demonstrable, establishing the existence of a mechanism of macrophage antibacterial activity independent of phox and NOS2.  (+info)

Role of Listeria monocytogenes exotoxins listeriolysin and phosphatidylinositol-specific phospholipase C in activation of human neutrophils. (2/3136)

Polymorphonuclear leukocytes (PMN) are essential for resolution of infections with Listeria monocytogenes. The present study investigated the role of the listerial exotoxins listeriolysin (LLO) and phosphatidylinositol-specific phospholipase C (PlcA) in human neutrophil activation. Different Listeria strains, mutated in individual virulence genes, as well as purified LLO were used. Coincubation of human neutrophils with wild-type L. monocytogenes provoked PMN activation, occurring independently of phagocytosis events, with concomitant elastase secretion, leukotriene generation, platelet-activating factor (PAF) synthesis, respiratory burst, and enhanced phosphoinositide hydrolysis. Degranulation and leukotriene formation were noted to be solely dependent on LLO expression, as these features were absent when the LLO-defective mutant EGD- and the avirulent strain L. innocua were used. These effects were fully reproduced by a recombinant L. innocua strain expressing LLO (INN+) and by the purified LLO molecule. LLO secretion was also required for PAF synthesis. However, wild-type L. monocytogenes was more potent in eliciting PAF formation than mutants expressing LLO, suggesting the involvement of additional virulence factors. This was even more obvious for phosphoinositide hydrolysis and respiratory burst: these events were provoked not only by INN+ but also by the LLO-defective mutant EGD- and by a recombinant L. innocua strain producing listerial PlcA. We conclude that human neutrophils react to extracellularly provided listerial exotoxins by rapid cell activation. Listeriolysin is centrally involved in triggering degranulation and lipid mediator generation, and further virulence factors such as PlcA apparently contribute to trigger neutrophil phosphoinositide hydrolysis and respiratory burst. In this way, listerial exotoxins may influence the host defense against infections with L. monocytogenes.  (+info)

Noncompetitive expansion of cytotoxic T lymphocytes specific for different antigens during bacterial infection. (3/3136)

Listeria monocytogenes is an intracellular bacterium that elicits complex cytotoxic T-lymphocyte (CTL) responses in infected mice. The responses of CTL populations that differ in antigen specificity range in magnitude from large, dominant responses to small, subdominant responses. To test the hypothesis that dominant T-cell responses inhibit subdominant responses, we eliminated the two dominant epitopes of L. monocytogenes by anchor residue mutagenesis and measured the T-cell responses to the remaining subdominant epitopes. Surprisingly, the loss of dominant T-cell responses did not enhance subdominant responses. While mice immunized with bacteria lacking dominant epitopes developed L. monocytogenes-specific immunity, their ability to respond to dominant epitopes upon rechallenge with wild-type bacteria was markedly diminished. Recall responses in mice immunized with wild-type or epitope-deficient L. monocytogenes showed that antigen presentation during recall infection is sufficient for activating memory cells yet insufficient for optimal priming of naive T lymphocytes. Our findings suggest that T-cell priming to different epitopes during L. monocytogenes infection is not competitive. Rather, T-cell populations specific for different antigens but the same pathogen expand independently.  (+info)

Dopamine beta-hydroxylase deficiency impairs cellular immunity. (4/3136)

Norepinephrine, released from sympathetic neurons, and epinephrine, released from the adrenal medulla, participate in a number of physiological processes including those that facilitate adaptation to stressful conditions. The thymus, spleen, and lymph nodes are richly innervated by the sympathetic nervous system, and catecholamines are thought to modulate the immune response. However, the importance of this modulatory role in vivo remains uncertain. We addressed this question genetically by using mice that lack dopamine beta-hydroxylase (dbh-/- mice). dbh-/- mice cannot produce norepinephrine or epinephrine, but produce dopamine instead. When housed in specific pathogen-free conditions, dbh-/- mice had normal numbers of blood leukocytes, and normal T and B cell development and in vitro function. However, when challenged in vivo by infection with the intracellular pathogens Listeria monocytogenes or Mycobacterium tuberculosis, dbh-/- mice were more susceptible to infection, exhibited extreme thymic involution, and had impaired T cell function, including Th1 cytokine production. When immunized with trinitrophenyl-keyhole limpet hemocyanin, dbh-/- mice produced less Th1 cytokine-dependent-IgG2a antitrinitrophenyl antibody. These results indicate that physiological catecholamine production is not required for normal development of the immune system, but plays an important role in the modulation of T cell-mediated immunity to infection and immunization.  (+info)

Infrarenal endoluminal bifurcated stent graft infected with Listeria monocytogenes. (5/3136)

Prosthetic graft infection as a result of Listeria monocytogenes is an extremely rare event that recently occurred in a 77-year-old man who underwent endoluminal stent grafting for infrarenal abdominal aortic aneurysm. The infected aortic endoluminal prosthesis was removed by means of en bloc resection of the aneurysm and contained endograft with in situ aortoiliac reconstruction. At the 10-month follow-up examination, the patient was well and had no signs of infection.  (+info)

Evaluation of accuracy and repeatability of identification of food-borne pathogens by automated bacterial identification systems. (6/3136)

The performances of five automated microbial identification systems, relative to that of a reference identification system, for their ability to accurately and repeatedly identify six common food-borne pathogens were assessed. The systems assessed were the MicroLog system (Biolog Inc., Hayward, Calif.), the Microbial Identification System (MIS; MIDI Inc., Newark, Del.), the VITEK system (bioMerieux Vitek, Hazelwood, Mo.), the MicroScan WalkAway 40 system (Dade-MicroScan International, West Sacramento, Calif.), and the Replianalyzer system (Oxoid Inc., Nepean, Ontario, Canada). The sensitivities and specificities of these systems for the identification of food-borne isolates of Bacillus cereus, Campylobacter jejuni, Listeria monocytogenes, Staphylococcus aureus, Salmonella spp., and verotoxigenic Escherichia coli were determined with 40 reference positive isolates and 40 reference negative isolates for each pathogen. The sensitivities of these systems for the identification of these pathogens ranged from 42.5 to 100%, and the specificities of these systems for the identification of these pathogens ranged from 32.5 to 100%. Some of the systems had difficulty correctly identifying the reference isolates when the results were compared to those from the reference identification tests. The sensitivity of MIS for the identification of S. aureus, B. cereus, E. coli, and C. jejuni, for example, ranged from 47.5 to 72. 5%. The sensitivity of the Microlog system for the identification of E. coli was 72.5%, and the sensitivity of the VITEK system for the identification of B. cereus was 42.5%. The specificities of four of the five systems for the identification of all of the species tested with the available databases were greater than or equal to 97.5%; the exception was MIS for the identification of C. jejuni, which displayed a specificity of 32.5% when it was tested with reference negative isolates including Campylobacter coli and other Campylobacter species. All systems had >80% sensitivities for the identification of Salmonella species and Listeria species at the genus level. The repeatability of these systems for the identification of test isolates ranged from 30 to 100%. Not all systems included all six pathogens in their databases; thus, some species could not be tested with all systems. The choice of automated microbial identification system for the identification of a food-borne pathogen would depend on the availability of identification libraries within the systems and the performance of the systems for the identification of the pathogen.  (+info)

The isoflavone genistein inhibits internalization of enteric bacteria by cultured Caco-2 and HT-29 enterocytes. (7/3136)

The dietary isoflavone genistein is the focus of much research involving its role as a potential therapeutic agent in a variety of diseases, including cancer and heart disease. However, there is recent evidence that dietary genistein may also have an inhibitory effect on extraintestinal invasion of enteric bacteria. To study the effects of genistein on bacterial adherence and internalization by confluent enterocytes, Caco-2 and HT-29 enterocytes (cultivated for 15-18 d and 21-24 d, respectively) were pretreated for 1 h with 0, 30, 100, or 300 micromol/L genistein, followed by 1-h incubation with pure cultures of Listeria monocytogenes, Salmonella typhimurium, Proteus mirabilis, or Escherichia coli. Pretreatment of Caco-2 and HT-29 enterocytes with genistein inhibited bacterial internalization in a dose-dependent manner (r = 0.60-0.79). Compared to untreated enterocytes, 1-h pretreatment with 300 micromol/L genistein was generally associated with decreased bacterial internalization (P < 0. 05) without a corresponding decrease in bacterial adherence. Using Caco-2 cell cultures, decreased bacterial internalization was associated with increased integrity of enterocyte tight junctions [measured by increased transepithelial electrical resistance (TEER)], with alterations in the distribution of enterocyte perijunctional actin filaments (visualized by fluorescein-labeled phalloidin), and with abrogation of the decreased TEER associated with S. typhimurium and E. coli incubation with the enterocytes (P < 0.01). Thus, genistein was associated with inhibition of enterocyte internalization of enteric bacteria by a mechanism that might be related to the integrity of the enterocyte tight junctions, suggesting that genistein might function as a barrier-sustaining agent, inhibiting extraintestinal invasion of enteric bacteria.  (+info)

Listeria monocytogenes phospholipase C-dependent calcium signaling modulates bacterial entry into J774 macrophage-like cells. (8/3136)

Listeria monocytogenes secretes several proteins that have been shown to contribute to virulence. Among these is listeriolysin O (LLO), a pore-forming hemolysin that is absolutely required for virulence. Two other virulence factors are phospholipases: a phosphatidylinositol-specific phospholipase C (PI-PLC [plcA]) and a broad-range PLC (plcB). Although mutations in plcA or plcB resulted in small increases in mouse 50% lethal dose (LD50), deletions in both genes resulted in a 500-fold increase in LD50. We have examined the role of these secreted proteins in host intracellular signaling in the J774 macrophage-like cell line. Measurements of cytosolic free calcium ([Ca2+]i) have revealed a rapid spike upon exposure of these cells to wild-type L. monocytogenes. This is followed by a second peak at 5 min and a third prolonged peak with a maximal [Ca2+]i of 800 to 1,000 nM. The pattern of calcium changes was greatly altered by deletion of any of the three virulence factors. An LLO mutant produced none of these elevations in [Ca2+]i; however, a transient elevation was observed whenever these bacteria entered the cell. A PI-PLC mutant produced a diminished single elevation in [Ca2+]i at 15 to 30 min. A broad-range PLC mutant produced only the first calcium spike. Studies with inhibitors suggested that the first elevation arises from influx of calcium from the extracellular medium through plasma membrane channels and that the second and third elevations come from release of Ca2+ from intracellular stores. We observed that internalization of wild-type bacteria and the broad-range PLC mutant was delayed for 5 to 10 min, but the LLO and PI-PLC mutants were internalized rapidly upon infection. Inhibitors that affected calcium signaling changed the kinetics of association of wild-type bacteria with J774 cells, the kinetics of entry, and the efficiency of escape from the primary phagosome.  (+info)

The control, survival, and growth of Listeria monocytogenes on food pr by Richelle Lynn Beverly"The control, survival, and growth of Listeria monocytogenes on food pr" by Richelle Lynn Beverly
Listeria monocytogenes, a ubiquitous foodborne pathogen, was recognized over 70 years ago. It is the source of the human disease listeriosis. The majority of Listeria monocytogenes that have been isolated from food product or human cases are of the serotypes ½ a, ½ b and 4b. Due to the recent outbreaks, recalls and deaths associated with Listeria monocytogenes in ready-to-eat meat products, the United States Department of Agriculture (USDA), Food Safety and Inspection Service (FSIS) on October 2, 2003 issued a directive for the control of Listeria monocytogenes on ready-to-eat products. The ready-to-eat food industry must impose a post-lethality treatment and/or growth inhibitor for Listeria monocytogenes on ready-to-eat products. The purpose of this study was to assess the use of different antimicrobial treatments for the inhibition of Listeria monocytogenes on the surface of food products. The utilization of natural ingredients such as cranberries as well as chemicals such as acidified sodium
Listeria monocytogenes exploits efferocytosis to promote cell-to-cell spread | NatureListeria monocytogenes exploits efferocytosis to promote cell-to-cell spread | Nature
The intracellular bacterial pathogen Listeria monocytogenes is shown to exploit efferocytosis-the process by which dead or dying cells are removed by phagocytosis-to promote cell-to-cell spread during infection. This study of the intracellular bacterial pathogen Listeria monocytogenes, a significant cause of foodborne illness, shows that it exploits the hosts efferocytosis system to promote cell-to-cell spread during infection. Efferocytosis is the process by which dead or dying cells are removed by phagocytosis and it relies in part on the receptors that bind to exofacial phosphatidylserine on the surface of cells or cellular debris following the loss of plasma membrane asymmetry. Listerial actin-based motility leads to the formation of protrusions at the cell surface of infected cells, eventually leading to uptake of bacteria by adjacent cells. These findings identify phosphatidylserine as a possible drug target in infections by L. monocytogenes and other bacteria using similar strategies of cell-to
LISTERIA MONOCYTOGENES/LISTERIA SPP. | FOOD MICROBIOLOGY | FAPAS PROFICIENCY TESTS | BioFront TechnologiesLISTERIA MONOCYTOGENES/LISTERIA SPP. | FOOD MICROBIOLOGY | FAPAS PROFICIENCY TESTS | BioFront Technologies
Listeria Monocytogenes Listeria Spp Food Microbiology Fapas Proficiency Tests Biofront Technologies. LISTERIA MONOCYTOGENES/LISTERIA SPP. IN MILK POWDER Distribution Date: 07/03/2017 LISTERIA MONOCYTOGENES/LISTERIA SPP. IN CHICKEN Distribution Date: 11/27/2017 LISTERIA MONOCYTOGENES/LISTERIA SPP. IN BEEF Distribution Date: 01/29/2018 LISTERIA MONOCYTOGENES/LISTERIA SPP. IN SPONGE SWAB Distribution Date: 02/08/2018
5.0 - Guidance for the Application of Health Canadas Policy on Listeria monocytogenes in Ready-to-Eat Foods - Food - Canadian...5.0 - Guidance for the Application of Health Canada's Policy on Listeria monocytogenes in Ready-to-Eat Foods - Food - Canadian...
The goal of Health Canadas Policy on Listeria monocytogenes, in Ready-to-Eat Foods (the Policy) is to protect the health of Canadian consumers, to provide guidance to industry and regulatory authorities regarding the verification and control of Listeria monocytogenes in Ready-to-Eat foods.
IJERPH | Free Full-Text | Evaluation of Three Swabbing Devices for Detection of Listeria monocytogenes on Different Types of...IJERPH | Free Full-Text | Evaluation of Three Swabbing Devices for Detection of Listeria monocytogenes on Different Types of...
Listeria monocytogenes can adhere to different types of food contact surfaces within a food processing environment. Therefore, environmental sampling devices should be capable of detecting unacceptable contamination. In this study, a sponge-stick, foam spatula and an environmental swab were evaluated on their ability to detect low concentrations of L. monocytogenes on different types of food contact surfaces. A cocktail of four L. monocytogenes serotypes was inoculated with a concentration of 100 CFU/250 cm2 onto stainless steel (SS), high density polyethylene (HDPE) and rubber surfaces in a 250 cm2 area. Immediately after inoculation and after 1 h exposure, the surfaces were swabbed with the different swabbing devices. The results of the study show only minor differences in the ability of the swabbing devices to detect L. monocytogenes. All devices were capable to detect the contamination immediately after inoculation. However, when the surfaces were allowed to air-dry for 1 h, L. monocytogenes was
A single amino acid in E-cadherin responsible for host specificity towards the human pathogen Listeria monocytogenes • Research...A single amino acid in E-cadherin responsible for host specificity towards the human pathogen Listeria monocytogenes • Research...
Human E-cadherin promotes entry of the bacterial pathogen Listeria monocytogenes into mammalian cells by interacting with internalin (InlA), a bacterial surface protein. Here we show that mouse E-cadherin, although very similar to human E-cadherin (85% identity), is not a receptor for internalin. By a series of domain-swapping and mutagenesis experiments, we identify Pro16 of E-cadherin as a residue critical for specificity: a Pro--|Glu substitution in human E-cadherin totally abrogates interaction, whereas a Glu--|Pro substitution in mouse E-cadherin results in a complete gain of function. A correlation between cell permissivity and the nature of residue 16 in E-cadherins from several species is established. The location of this key specificity residue in a region of E-cadherin not involved in cell-cell adhesion and the stringency of the interaction demonstrated here have important consequences not only for the understanding of internalin function but also for the choice of the animal model to be used
ASMscience | Listeria monocytogenes IASMscience | Listeria monocytogenes I
Although infection with the intracellular bacterium Listeria monocytogenes can result in severe illnesses such as sepsis and meningitis in immunocompromised people, a much more common outcome is control and clearance of the organism without serious malady. L. monocytogenes is able to infect common laboratory animals such as the mouse, immunologists have been able to dissect this host-pathogen interplay and elucidate the immune functions required to contain and eliminate L. monocytogenes infection. Importantly, the mouse model of L. monocytogenes infection has yielded discoveries that are not only specific to this host-pathogen interaction but also help define fundamental concepts of innate and adaptive immunity. The natural route of L. monocytogenes infection in humans is via the gut after consumption of contaminated food products. However, infection of mice in this manner requires an extremely large inoculum and often results in asynchronous systemic infections, which complicate experimental design.
A Multiorgan Trafficking Circuit Provides Purifying Selection of Listeria monocytogenes Virulence Genes | mBioA Multiorgan Trafficking Circuit Provides Purifying Selection of Listeria monocytogenes Virulence Genes | mBio
Listeria monocytogenes can cause a life-threatening illness when the foodborne pathogen spreads beyond the intestinal tract to distant organs. Many aspects of the intestinal phase of L. monocytogenes pathogenesis remain unknown. Here, we present a foodborne infection model using C57BL/6 mice that have been pretreated with streptomycin. In this model, as few as 100 L. monocytogenes CFU were required to cause self-limiting enterocolitis, and systemic dissemination followed previously reported routes. Using this model, we report that listeriolysin O (LLO) and actin assembly-inducing protein (ActA), two critical virulence determinants, were necessary for intestinal pathology and systemic spread but were dispensable for intestinal growth. Sequence tag-based analysis of microbial populations (STAMP) was used to investigate the within-host population dynamics of wild-type and LLO-deficient strains. The wild-type bacterial population experienced severe bottlenecks over the course of infection, and by 5 ...
Application of Non-linear Models to Predict Inhibition Effects of Various Plant Hydrosols on Listeria monocytogenes Inoculated...Application of Non-linear Models to Predict Inhibition Effects of Various Plant Hydrosols on Listeria monocytogenes Inoculated...
In this study, we studied the effects of some plant hydrosols obtained from bay leaf, black cumin, rosemary, sage, and thyme in reducing Listeria monocytogenes on the surface of fresh-cut apple cubes. Adaptive neurofuzzy inference system (ANFIS), artificial neural network (ANN), and multiple linear regression (MLR) models were used for describing the behavior of L. monocytogenes against the hydrosol treatments. Approximately 1-1.5 log CFU/g decreases in L. monocytogenes counts were observed after individual hydrosol treatments for 20 min. By extending the treatment time to 60 min, thyme, sage, or rosemary hydrosols eliminated L. monocytogenes, whereas black cumin and bay leaf hydrosols did not lead to additional reductions. In addition to antibacterial measurements, the abilities of ANFIS, ANN, and MLR models were compared with respect to estimation of the survival of L. monocytogenes. The root mean square error, mean absolute error, and determination coefficient statistics were used as ...
Listeria monocytogenes isolates carrying virulence-attenuating mutations in internalin A are commonly isolated from ready-to...Listeria monocytogenes isolates carrying virulence-attenuating mutations in internalin A are commonly isolated from ready-to...
TY - JOUR. T1 - Listeria monocytogenes isolates carrying virulence-attenuating mutations in internalin A are commonly isolated from ready-to-eat food processing plant and retail environments. AU - Van Stelten, A.. AU - Roberts, A. R.. AU - Manuel, C. S.. AU - Nightingale, K. K.. PY - 2016/10. Y1 - 2016/10. N2 - Listeria monocytogenes is a human foodborne pathogen that may cause an invasive disease known as listeriosis in susceptible individuals. Internalin A (InlA; encoded by inlA) is a virulence factor that facilitates crossing of host cell barriers by L. monocytogenes. At least 19 single nucleotide polymorphisms (SNPs) in inlA that result in a premature stop codon (PMSC) have been described worldwide. SNPs leading to a PMSC in inlA have been shown to be causally associated with attenuated virulence. L. monocytogenes pathogens carrying virulence-attenuating (VA) mutations in inlA have been commonly isolated from ready-to-eat (RTE) foods but rarely have been associated with human disease. This ...
Development of a 16S rRNA-based oligomer probe specific for Listeria monocytogenes. | Applied and Environmental MicrobiologyDevelopment of a 16S rRNA-based oligomer probe specific for Listeria monocytogenes. | Applied and Environmental Microbiology
Crude rRNA was isolated from Listeria monocytogenes, L. innocua, and L. ivanovii and sequenced by a reverse transcriptase method. Only two sequence regions were found to differ for L. monocytogenes versus L. innocua or L. ivanovii. Two oligonucleotide probes (RL-1 and RL-2) complementary to these two regions of rRNA of L. monocytogenes were synthesized. The RL-1 probe had one base while the RL-2 probe had two bases which differed for L. monocytogenes versus L. innocua and L. ivanovii. Use of a dried gel hybridization in place of Northern (RNA) hybridization or dot blot hybridization indicated that the RL-2 probe hybridized with all 36 strains of L. monocytogenes tested but not with 6 other Listeria species and 11 other bacteria tested. The RL-2 probe is specific for L. monocytogenes, while the RL-1 probe showed some cross-reactions with other Listeria species. An alkaline phosphatase-labeled RL-2 probe could be used in a dot blot hybridization test and gave good results, but a 32P-labeled RL-2 ...
Reducing Listeria monocytogenes on roast beef with acidified sodium chloriteReducing Listeria monocytogenes on roast beef with acidified sodium chlorite
Listeria monocytogenes is a bacterium that can cause illness when consumers eat refrigerated, ready-to-eat foods contaminated with this micro-organism. Eating foods contaminated with L. monocytogenes normally causes flu-like symptoms in healthy adults. This disease is more serious for elderly adults and adults with compromised immune systems and can cause menin-gitis. In pregnant women, the disease may cause spontaneous abortions or stillborn babies.. From July 1998 to January 1999, four U.S. companies recalled ready-to-eat meats after L. monocytogenes was found in their products. Two major recalls involved 30 million pounds each. According to the Centers for Disease Control, tainted meat traced to a Michigan plant was responsible for 16 deaths, six miscarriages and 100 illnesses. As a result of this outbreak, the U.S. Department of Agricultures Food Safety and Inspection Service (FSIS) addressed the problem of L. monocytogenes contamination in a directive published in the Federal Register in ...
The Alternative Sigma Factor σB and the Virulence Gene Regulator PrfA Both Regulate Transcription of Listeria monocytogenes...The Alternative Sigma Factor σB and the Virulence Gene Regulator PrfA Both Regulate Transcription of Listeria monocytogenes...
The gram-positive bacterium Listeria monocytogenes is recognized as an important human food-borne pathogen, causing manifestations ranging from mild febrile gastroenteritis to severe invasive infections. High mortality rates are associated with systemic infections, which occur predominantly among pregnant women, the immunocompromised, and the elderly (19, 24). In animals, L. monocytogenes infections can result in a variety of manifestations, including abortion, encephalitis, septicemia, and, less commonly, keratoconjunctivitis and mastitis (43).. A variety of L. monocytogenes surface proteins, including several members of the internalin family (11), are important for facilitating interactions between this pathogen and mammalian host cells. For example, internalin A (InlA) promotes invasion of human nonphagocytic cells that express the host cell receptor E-cadherin, such as the Caco-2 epithelial cell line (45). InlB mediates entry into several host cell types, including hepatocytes and several ...
An outbreak of an unusual strain of Listeria monocytogenes infection in North-East Scotland<...An outbreak of an unusual strain of Listeria monocytogenes infection in North-East Scotland<...
TY - JOUR. T1 - An outbreak of an unusual strain of Listeria monocytogenes infection in North-East Scotland. AU - Okpo, Emmanuel. AU - Leith, Jayne. AU - Smith-Palmer, Alison. AU - Bell, John. AU - Parks, Duncan. AU - Browning, Fiona. AU - Byers, Lynn. AU - Corrigan, Helen. AU - Webster, Diana. AU - Karcher, Anne M. AU - Murray, Andrew. AU - Storey, Tom. N1 - Acknowledgements The authors would like to thank Kerry Parrott and Pauline Fuchs from the Aberdeen Scientific Services Laboratory (ASSL) for the analysis and testing of food and environmental samples. We also thank Corinne Amar, Craig Swift and Adedoyin Awofisayo from PHE Foodborne Pathogens Reference Services, London, for providing expert advice and molecular typing results in a timely manner. Funding No funding sources.. PY - 2015/11. Y1 - 2015/11. N2 - Listeria monocytogenes infection is an important cause of illness and hospitalization in vulnerable individuals. In the present study, we describe a community outbreak of Listeria ...
Capacity of ivanolysin O to replace listeriolysin O in phagosomal escape and in vivo survival of Listeria monocytogenes |...Capacity of ivanolysin O to replace listeriolysin O in phagosomal escape and in vivo survival of Listeria monocytogenes |...
Listeriolysin O (LLO, hly-encoded) is a major virulence factor secreted by the pathogen Listeria monocytogenes. The amino acid sequence of LLO shows a high degree of similarity with that of ivanolysin O (ILO), the cytolysin secreted by the ruminant pathogen Listeria ivanovii. Here, it was tested whether ILO could functionally replace LLO by expressing the gene encoding ILO under the control of the hly promoter, in an hly-deleted strain of L. monocytogenes. It is shown that ILO allows efficient phagosomal escape of L. monocytogenes in both macrophages and hepatocytes. Moreover, expression of ILO is not cytotoxic and promotes normal intracellular multiplication. In vivo, the ILO-expressing strain can multiply and persist for several days in the liver of infected mice but is unable to survive in the spleen. This work underscores the key role played by the cytolysin in the virulence of pathogenic Listeria.
Listeria Monocytogenes: Incidence, Growth Behavior and Control - Nova Science PublishersListeria Monocytogenes: Incidence, Growth Behavior and Control - Nova Science Publishers
This book provides readers with the latest developments in Listeria Monocytogenes research. Topics covered include its growth in biofilms in the food industry; molecular mechanisms involved in the food-related subsistence of Listeria Monocytogenes; an epidemiological update and the control possibilities for outbreaks of L. Monocytogenes; how to control L. Monocytogenes biofilms on food contact surfaces; methods for killing activities of macrophages against L. Monocytogenes infection; and a review of some of the most exciting developments in the analysis of the interaction between L. Monocytogenes and the host GI tract ...
Listeria monocytogenes serotype 4a 30S ribosomal protein S19 (rpsS) GENTAUR-58b3878a3b838 | GentaursearchListeria monocytogenes serotype 4a 30S ribosomal protein S19 (rpsS) GENTAUR-58b3878a3b838 | Gentaursearch
Listeria monocytogenes serotype 4a 30S ribosomal protein S19 (rpsS) datasheet and description hight quality product and Backed by our Guarantee
Phage resistance at the cost of virulence: Listeria monocytogenes serovar 4b requires galactosylated teichoic acids for InlB...Phage resistance at the cost of virulence: Listeria monocytogenes serovar 4b requires galactosylated teichoic acids for InlB...
1. Maertens de Noordhout C, Devleesschauwer B, Angulo FJ, Verbeke G, Haagsma J, Kirk M, et al. The global burden of listeriosis: a systematic review and meta-analysis. Lancet Infect Dis. 2014;14(11):1073-82. doi: 10.1016/S1473-3099(14)70870-9 25241232. 2. Charlier C, Perrodeau É, Leclercq A, Cazenave B, Pilmis B, Henry B, et al. Clinical features and prognostic factors of listeriosis: the MONALISA national prospective cohort study. Lancet Infect Dis. 2017;17(5):510-9. doi: 10.1016/S1473-3099(16)30521-7 28139432. 3. Orsi RH, Bakker HC de., Wiedmann M. Listeria monocytogenes lineages: Genomics, evolution, ecology, and phenotypic characteristics. Int J Med Microbiol. 2011;301(2):79-96. doi: 10.1016/j.ijmm.2010.05.002 20708964. 4. Kamisango K, Saiki I, Tanio Y, Okumura H, Araki Y, Sekikawa I, et al. Structures and biological activities of peptidoglycans of Listeria monocytogenes and Propionibacterium acnes. J Biochem. 1982;92(1):23-33. doi: 10.1093/oxfordjournals.jbchem.a133918 6811573. 5. Brown S, ...
The first 20 codons of the prfA-mRNA are required for efficient translation in Listeria monocytogenesThe first 20 codons of the prfA-mRNA are required for efficient translation in Listeria monocytogenes
The Gram-positive human pathogen Listeria monocytogenes uses a wide range of virulence factors for its pathogenesis. The majority of its virulence genes are encoded on a 9-kb pathogenicity island and are controlled by the transcriptional activator PrfA. Expression of these genes is maximal at 37°C and minimal at 30°C in a mechanism involving an RNA thermosensor. This thesis brings up different aspects of RNA-mediated regulation, including regulatory RNA structures within coding mRNA controlling expression to 5-untranslated RNA (5´-UTR) that controls downstream genes (cis-acting) as well as small non-coding RNAs (ncRNAs) that bind other target RNA (trans-acting).. We investigated the importance of the coding region of the prfA-mRNA for its expression. Various lengths of prfA-mRNA were fused with reporter genes. Our finding suggested that the first 20 codons of prfA-mRNA were essential for efficient translation in Listeria monocytogenes. Translation of the shorter constructs was shown to be ...
Permalien vers Murinization of internalin extends its receptor repertoire, altering Listeria monocytogenes cell tropism and...Permalien vers Murinization of internalin extends its receptor repertoire, altering Listeria monocytogenes cell tropism and...
Lien vers Pubmed [PMID] - 23737746. PLoS Pathog. 2013;9(5):e1003381. Listeria monocytogenes (Lm) is an invasive foodborne pathogen that leads to severe central nervous system and maternal-fetal infections. Lm ability to actively cross the intestinal barrier is one of its key pathogenic properties. Lm crosses the intestinal epithelium upon the interaction of its surface protein internalin (InlA) with its host receptor E-cadherin (Ecad). InlA-Ecad interaction is species-specific, does not occur in wild-type mice, but does in transgenic mice expressing human Ecad and knock-in mice expressing humanized mouse Ecad. To study listeriosis in wild-type mice, InlA has been "murinized" to interact with mouse Ecad. Here, we demonstrate that, unexpectedly, murinized InlA (InlA(m)) mediates not only Ecad-dependent internalization, but also N-cadherin-dependent internalization. Consequently, InlA(m)-expressing Lm targets not only goblet cells expressing luminally-accessible Ecad, as does Lm in humanized mice, ...
Listeria Monocytogenes: Its discovery and naming | Earthworm ExpressListeria Monocytogenes: Its discovery and naming | Earthworm Express
Listeria Monocytogenes: Its discovery and naming. Eben van Tonder 16 December 2017 Cape Town Introduction The discovery and naming of the Listeria monocytogenes are instructive with a very interesting South African connection and a controversy associated with it in that there is disagreement in terms of who is being honoured in the name. Monocytogenes The…
Listeria monocytogenes</i> in Chicken...Listeria monocytogenes</i> in Chicken...
Listeria monocytogenes is the causative agent of listeriosis, an infection that gives rise to bacteremia and meningitis that can be propagated to humans via food contamination. The chicken-meat and derivatives processing industries are common sites of this pathogen, and the great challenge is in controlling this hazard to avoid economic and public health losses. A literature review on L. monocytogenes and implications to the chicken supply chain, poultry slaughterhouses, and public health was conducted. The review was compiled with the main papers published around the world in the last 15 years containing the key words Listeria monocytogenes, poultry, meat, chicken, broilers, and listeriosis, using the main publishers of online journals. The collected information was discussed and it was concluded that poultry can be asymptomatic carriers of L. monocytogenes and introduce contamination in slaughterhouses, which can become a persistent problem in poultry slaughterhouses due to its capacity to form
Listeria monocytogenes (Murray et al.) Pirie ATCC ® BAA-679D-5&traListeria monocytogenes (Murray et al.) Pirie ATCC ® BAA-679D-5&tra
Listeria monocytogenes ATCC ® BAA-679D-5™ Designation: Genomic DNA from Listeria monocytogenes Strain EGDe TypeStrain=False Application: Enteric Research
Flick of a switch : regulatory mechanisms allowing Listeria monocytogenes to transition from a saprophyte to a killerFlick of a switch : regulatory mechanisms allowing Listeria monocytogenes to transition from a saprophyte to a killer
In contrast to obligate intracellular pathogens that can remain in relatively stable host-associated environments, the soil-living bacterial pathogen Listeria monocytogenes has to sense and respond to physical and chemical cues in a variety of quite different niches. In particular, the bacterium has to survive the dramatic transition from its saprophytic existence to life within the host where nutritional stress, increased temperature, acidity, osmotic stress and the host defences present a new and challenging landscape. This review focuses on the sB and PrfA regulatory systems used by L. monocytogenes to sense the changing environment and implement survival mechanisms that help to overcome the disparate conditions within the host, but also to switch from a harmless saprophyte to an impressively effective pathogen.. ...
Temperature-Dependent Phage Resistance in Listeria monocytogenes Epidemic Clone II Strains - OpenThesisTemperature-Dependent Phage Resistance in Listeria monocytogenes Epidemic Clone II Strains - OpenThesis
Of at least 400 Listeria phages, only a few have been characterized as having wide host range among listeriae and no phages have been isolated from the environment of food processing plants, where contamination of highly processed, ready to eat foods is likely to occur. To understand the ecology of listeriaphage and Listeria monocytogenes in processing plant environments, we pursued the isolation of Listeria-specific phages from environmental samples from four turkey-processing plants in the United States. Nine of twelve isolated phages showed wide host range and the majority of L. monocytogenes of the serotype 4b complex (serotypes 4b, 4d, 4e) could be readily infected by these wide host range phages, but many isolates of other serotypes (1/2a or 3a, and 1/2b or 3b), which represented the majority of L. monocytogenes isolated from the environmental samples, were resistant to infection. L. monocytogenes epidemic clone II (ECII) has been responsible for two multistate outbreaks in the United ...
Get PDF - Behavior of Foodborne Pathogens Listeria monocytogenes and Staphylococcus aureus in Mixed-Species Biofilms Exposed to...Get PDF - Behavior of Foodborne Pathogens Listeria monocytogenes and Staphylococcus aureus in Mixed-Species Biofilms Exposed to...
Oxaran, V.; Dittmann, K.Kiesbye.; Lee, S.H.I.; Chaul, Líza.Toubas.; Fernandes de Oliveira, C.Augusto.; Corassin, C.Humberto.; Alves, Vínia.Farias.; De Martinis, E.Cristina.Pereira.; Gram, L., 2018: Behavior of Foodborne Pathogens Listeria monocytogenes and Staphylococcus aureus in Mixed-Species Biofilms Exposed to Biocides
Listeria monocytogenes (Murray et al.) Pirie ATCC ® 19115D-5™Listeria monocytogenes (Murray et al.) Pirie ATCC ® 19115D-5™
Listeria monocytogenes ATCC ® 19115D-5™ Designation: Genomic DNA from Listeria monocytogenes strain Li 2 TypeStrain=False Application:
Examination of food chain-derived Listeria monocytogenes strains of different serotypes reveals considerable diversity in inlA...Examination of food chain-derived Listeria monocytogenes strains of different serotypes reveals considerable diversity in inlA...
Examination of food chain-derived Listeria monocytogenes strains of different serotypes reveals considerable diversity in inlA genotypes, mutability, and adaptation to cold temperatures.
Cold shock proteins contribute to the regulation of listeriolysin o production in listeria monocytogenes - Zurich Open...Cold shock proteins contribute to the regulation of listeriolysin o production in listeria monocytogenes - Zurich Open...
Cold shock proteins (Csps) are multifunctional nucleic acid binding proteins used to regulate a wide range of gene expression responses in bacteria. We report here that Csps regulate the production of the pore-forming cytolysin listeriolysin (LLO) and hemolysis phenotypes in Listeria monocytogenes. A triple csp gene deletion mutant incapable of producing any Csps, as well as double csp gene deletion mutants only producing either CspA or CspD, caused less hemolysis and produced lower LLO concentration. On the other hand, another double csp gene deletion mutant that produces CspB retained hemolysis and LLO production levels that are similar to the parental wild-type strain. Transcription analysis showed that in absence of all three csp genes or cspB alone, L. monocytogenes cells have decreased levels of hly gene transcripts, which code for the synthesis of LLO proteins. A comparative examination of mRNA stability showed that hly transcripts were more rapidly degraded in L. monocytogenes triple csp ...
General Stress Transcription Factor ςB and Its Role in Acid Tolerance and Virulence ofListeria monocytogenes | Journal of...General Stress Transcription Factor ςB and Its Role in Acid Tolerance and Virulence ofListeria monocytogenes | Journal of...
The gene encoding the general stress transcription factor ςB in the gram-positive bacterium Listeria monocytogenes was isolated with degenerate PCR primers followed by inverse PCR amplification. Evidence for gene identification includes the following: (i) phylogenetic analyses of reported amino acid sequences for ςB and the closely related ςFproteins grouped L. monocytogenes ςB in the same cluster with the ςB proteins from Bacillus subtilis and Staphylococcus aureus, (ii) the gene order in the 2,668-bp portion of the L. monocytogenes sigBoperon is rsbU-rsbV-rsbW-sigB-rsbX and is therefore identical to the order of the last five genes of the B. subtilis sigB operon, and (iii) an L. monocytogenesςB mutant had reduced resistance to acid stress in comparison with its isogenic parent strain. The sigB mutant was further characterized in mouse models of listeriosis by determining recovery rates of the wild-type and mutant strains from livers and spleens following intragastric or intraperitoneal ...
Safety Study of Recombinant Listeria Monocytogenes(Lm)Based Vaccine Virus Vaccine to Treat Oropharyngeal Cancer - Full Text...Safety Study of Recombinant Listeria Monocytogenes(Lm)Based Vaccine Virus Vaccine to Treat Oropharyngeal Cancer - Full Text...
It is the investigators intention to investigate whether a specially designed vaccine, based on a genetically modified strain of the bacterium Listeria monocytogenes and called ADXS11-001 is safe to use and is able to boost the immune system of patients presenting with Human Papilloma Virus (HPV) associated oropharyngeal cancer (OPSCC). It is hoped that the vaccine will boost the immune system so that immune cells with cell killing properties are able to attack any cancer cells remaining after the patients have been treated. However, the vaccine is so novel the investigators are not sure whether it is able to do this and before they can answer that question in a larger group of patients they need to make sure that the vaccine is safe to use and has some effect on the immune system in the patients for whom they intend its ultimate use. In a previous study, patients with incurable cervix cancer which is caused by the same virus, were vaccinated with ADXS11-001. Although all patients vaccinated ...
Listeria Monocytogenes: Factory Cleaning, Thermal Inactivation and Acid Tolerance Response | Earthworm ExpressListeria Monocytogenes: Factory Cleaning, Thermal Inactivation and Acid Tolerance Response | Earthworm Express
Listeria Monocytogenes: Factory Cleaning, Thermal Inactivation and Acid Tolerance Response By Eben van Tonder 6 January 2018 (Update: 2/2018) Summary In light of the unprecedented listeria outbreak in South Africa, we examine processes and procedures at the cutting plant, factory cleaning, thermal inactivation and acid tolerance response of Listeria monocytogenes. We question everything we do…
Magiran | Biochemical and Molecular Identification of Listeria monocytogenes and Escherichia coli in the Raw Milk Samples...Magiran | Biochemical and Molecular Identification of Listeria monocytogenes and Escherichia coli in the Raw Milk Samples...
BackgroundMilk is an essential human nutrient, and the monitoring of its sanitation is vital during transportation and storage. The present study aimed to assess bacteriological contamination with Escherichia coli and Listeria monocytogenes in the raw milk samples of the dairy farms in Golestan province, Iran. MethodsIn total, 100 samples were collected from dairy farms in hot and cold seasons. The frequency of E. coli and L. monocytogenes was determined using biochemical tests and polymerase chain reaction (PCR). ResultsThe biochemical tests indicated that 28% and 27% of the samples were contaminated with E. coli and L. monocytogenes based on the culture-dependent methods, respectively. In addition, 35 and 40 samples were contaminated with E. coli and L. monocytogenes based on PCR, respectively. PCR had higher sensitivity compared to the biochemical tests (P ˂ 0.05). E. coli and L. monocytogenes contamination was significantly higher in traditional dairy farms than industrial dairy farms (P ˂ 0.05).
Critical roles of neutrophils in host defense against experimental systemic infections of mice by Listeria monocytogenes,...Critical roles of neutrophils in host defense against experimental systemic infections of mice by Listeria monocytogenes,...
This study shows that neutrophils are critical for combating experimental systemic infections of mice by the bacterial pathogens Listeria monocytogenes, Salmonella typhimurium, and Yersinia enterocolitica. It shows that mice rendered neutropenic by treatment with the granulocyte-depleting monoclonal antibody RB6-8C5 are far more susceptible than immunocompetent mice to infection with each of these three pathogens. Compared to immunocompetent mice, neutropenic mice exhibit several defects in their antibacterial capabilities. Firstly, the immediate inactivation of Listeria, Salmonella, or Yersinia that initially implants in the livers and spleens that occurs in immunocompetent mice is abolished in these organs in neutropenic mice. Secondly, unlike immunocompetent mice, neutropenic mice neither control the subsequent proliferation of the inoculated bacteria in the livers and spleens nor prevent dissemination of infection to other organs. Thirdly, mice rendered neutropenic develop a generalized ...
CRITERION UVM Modified Listeria Enrichment BrothCRITERION UVM Modified Listeria Enrichment Broth
1. Murray, E.G.D., R.A. Webb, and M.B.R. Swann. 1926. A disease of rabbits characterized by large mononuclear leucocytosis caused by a hitherto undescribed bacillus Bacterium monocytogenes. J. Path. Bact.; 29:407-0439.. 2. Monk, J.D., R.S. Clavero, L.R. Beuchat, M.P. Doyle and R.E. Brackett. 1994. Irradiation inactivation of Listeria monocytogenes and Staphylococcus aureus in low- and high-fat, frozen and refrigerated ground beef. J. Food Prot.; 57:969-974.. 3. Wehr, H.M. 1987. Listeria monocytogenes - a current dilemma special report. J. Assoc. Off. Anal. Chem.; 70:769-772.. 4. Bremer, P.J., and C.M. Osborne. 1995. Thermal-death times of Listeria monocytogenes in green shell mussels (Perna canaliculus) prepared for hot smoking. J. Food Prot.; 58:604-608.. 5. Grau, F.H., and P.B. Vanderlinde. 1992. Occurrence, numbers, and growth of Listeria monocytogenes on some vacuum-packaged processed meats. J. Food Prot.; 55:4-7.. 6. Patel, J.R., C.A. Hwang, L.R. Beuchat, M.P. Doyle, and R.E. Brackett. ...
Određivanje prisutnosti bakterije Listeria monocytogenes u svježjem kravljem siru imunoenzimskom metodom | Croatian Digital...Određivanje prisutnosti bakterije Listeria monocytogenes u svježjem kravljem siru imunoenzimskom metodom | Croatian Digital...
The aim of this study was to determine the presence of Listeria spp. Immunoassay method in samples of fresh cow cheese, and from the obtained positive samples with Horizontal method for the detection and enumeration of cells and biochemical prove the presence of species Listeria monocytogenes. Also is need to compare the results obtained by Immunoassay method and by Horizontal method. It research involve a total of 30 randomly selected samples of fresh cow cheese that are available in Zagreb on markets. The results obtained are compared with the criteria set forth in the applicable legislation of the Republic of Croatian. In one of the 30 samples is showed presence of Listeria monocytogenes, and in three samples was determined by the presence of Listeria innocua ...
Toxins | Free Full-Text | Distinct Neurotoxicity Profile of Listeriolysin O from Listeria monocytogenesToxins | Free Full-Text | Distinct Neurotoxicity Profile of Listeriolysin O from Listeria monocytogenes
Cholesterol-dependent cytolysins (CDCs) are protein toxins that originate from Gram-positive bacteria and contribute substantially to their pathogenicity. CDCs bind membrane cholesterol and build prepores and lytic pores. Some effects of the toxins are observed in non-lytic concentrations. Two pathogens, Streptococcus pneumoniae and Listeria monocytogenes, cause fatal bacterial meningitis, and both produce toxins of the CDC family-pneumolysin and listeriolysin O, respectively. It has been demonstrated that pneumolysin produces dendritic varicosities (dendrite swellings) and dendritic spine collapse in the mouse neocortex, followed by synaptic loss and astrocyte cell shape remodeling without elevated cell death. We utilized primary glial cultures and acute mouse brain slices to examine the neuropathological effects of listeriolysin O and to compare it to pneumolysin with identical hemolytic activity. In cultures, listeriolysin O permeabilized cells slower than pneumolysin did but still initiated non
Alteration of epithelial cell lysosomal integrity induced by bacterial cholesterol-dependent cytolysins. - Institut PasteurAlteration of epithelial cell lysosomal integrity induced by bacterial cholesterol-dependent cytolysins. - Institut Pasteur
Bacterial pathogens can interfere during infection with host cell organelles, such as mitochondria, the endoplasmic reticulum-Golgi system or nuclei. As important cellular functions are often compartmentalized in these organelles, their targeting allows pathogens to manipulate key host functions during infection. Here, we identify lysosomes as a new class of organelles targeted by the pathogenic bacterium Listeria monocytogenes. We demonstrate that extracellular Listeria, via secretion of the pore-forming toxin listeriolysin O, alters lysosomal integrity in epithelial cells but not in macrophages. Listeriolysin O induces lysosomal membrane permeabilization and release of lysosomal content, such as cathepsins proteases, which remain transiently active in the host cytosol. We furthermore show that other bacterial pore-forming toxins, such as perfringolysin O and pneumolysin, also induce lysosomes alteration. Together, our data unveil a novel activity of bacterial cholesterol-dependent cytolysins.
topic:Listeria monocytogenes - drug effects - genetics - isolation & purification found 1 record • Arctic Healthtopic:"Listeria monocytogenes - drug effects - genetics - isolation & purification" found 1 record • Arctic Health
Five grams of seafood products were inoculated with one to 500 viable or 10(9) heat-killed cells of Listeria monocytogenes. The presence of the pathogen was detected by the polymerase chain reaction (PCR) with primers specific for fragments of the listeriolysin O (hly) gene (two sets) and for the invasion-associated protein (iap) gene (one set). For DNA preparation, boiling, either alone or in combination with lysozyme and proteinase K treatment, was not always sufficient to lyse L. monocytogenes, while treatment with Triton X-100 produced consistently good DNA suitable for amplification. To avoid false-negative and false-positive results, 48 h incubations were necessary and a subculturing step after an initial 24 h incubation greatly improved the results. The primers that amplified regions of the listeriolysin O gene gave clearer and stronger products than primers for the invasion-associated protein gene. Using this method we were able to detect one to five L. monocytogenes cells in 5 g of ...
topic:Listeria monocytogenes - drug effects found 1 record • Arctic Healthtopic:"Listeria monocytogenes - drug effects" found 1 record • Arctic Health
Five grams of seafood products were inoculated with one to 500 viable or 10(9) heat-killed cells of Listeria monocytogenes. The presence of the pathogen was detected by the polymerase chain reaction (PCR) with primers specific for fragments of the listeriolysin O (hly) gene (two sets) and for the invasion-associated protein (iap) gene (one set). For DNA preparation, boiling, either alone or in combination with lysozyme and proteinase K treatment, was not always sufficient to lyse L. monocytogenes, while treatment with Triton X-100 produced consistently good DNA suitable for amplification. To avoid false-negative and false-positive results, 48 h incubations were necessary and a subculturing step after an initial 24 h incubation greatly improved the results. The primers that amplified regions of the listeriolysin O gene gave clearer and stronger products than primers for the invasion-associated protein gene. Using this method we were able to detect one to five L. monocytogenes cells in 5 g of ...
Fluorescent Microspheres as Position Markers to Track the Rotation of Listeria monocytogenes during Actin-Based MotilityFluorescent Microspheres as Position Markers to Track the Rotation of Listeria monocytogenes during Actin-Based Motility
68 Lauer, P., J. A. Theriot, J. Skoble, M. D. Welch, and D. A. Portnoy. 2001. Systematic mutational analysis of the amino-terminal domain of the Listeria monocytogenes ActA protein reveals novel functions in actin-based motility. Mol. Microbiol. 42:1163-1177. Li, R. 1997. Bee1, a yeast protein with homology to Wiskott-Aldrich syndrome protein, is critical for the assembly of cortical actin cytoskeleton. J. Cell Biol. 136:649-658. Loisel, T. P., R. Boujamaa, D. Pantaloni, and M. F. Carlier. 1999. Reconstitution of actin-based motility of Listeria and Shigella using pure proteins. Nature. 401:613-616. Machesky, L. M., R. D. Mullins, H. N. Higgs, D. A. Kaiser, L. Blanchoin, R. C. May, M. E. Hall, and T. D. Pollard. 1999. Scar, a WASp-related protein, activates nucleation of actin filaments by the Arp 2/3 complex. Proc. Natl. Acad. Sci. USA. 96:3739-3744. Makino, S., C. Sasakawa, T. Kamata, and M. Yoshikawa. 1986. A genetic determinant required for continuous reinfection of adjacent cells on a large ...
Typing of Listeria monocytogenes isolates originating from the food processing industry with automated ribotyping and pulsed...Typing of Listeria monocytogenes isolates originating from the food processing industry with automated ribotyping and pulsed...
A total of 486 Listeria monocytogenes isolates originating from 17 Finnish food processing plants (representing meat, poultry, fish, and dairy production) were collected and typed by automated ribotyping using EcoRI as the restriction enzyme. The isolates were divided into 16 different ribotypes (RTs). Some of these isolates (121), representing all EcoRI types and 16 food plants, were subjected to ribotyping with the PvuII enzyme, to pulsed-field gel electrophoresis (PFGE) typing with AscI and SmaI restriction enzymes, and to serotyping with O-antigen antisera. Nineteen ribotypes were generated with PvuII, 42 macrorestriction patterns were generated with AscI and 24 with SmaI, and three serotypes were generated with antisera. When the results were combined, the overall number of RTs was 23, and that of the PFGE types was 46. Thus, the overall discrimination power of PFGE was higher (discrimination index [DI] 0.966) than that of ribotyping (DI 0.906). The most common serotype (90.1% of the isolates) was
Listeria monocytogenes virulence and pathogenicity, a food safety perspectiveListeria monocytogenes virulence and pathogenicity, a food safety perspective
Several virulence factors of Listeria monocytogenes have been identified and extensively characterized at the molecular and cell biologic levels, including the hemolysin (listeriolysin O), two distinct phospholipases, a protein (ActA), several internalins, and others. Their study has yielded an impr …
A Mouse Model of Foodborne Listeria monocytogenes Infection - Current ProtocolsA Mouse Model of Foodborne Listeria monocytogenes Infection - Current Protocols
Listeria monocytogenes causes foodborne disease in humans that ranges in severity from mild, self‐limiting gastroenteritis to life‐threatening systemic infections of the blood, brain, or placenta
Functional assembly of two membrane-binding domains in listeriolysin O, the cytolysin of Listeria monocytogenes | Microbiology...Functional assembly of two membrane-binding domains in listeriolysin O, the cytolysin of Listeria monocytogenes | Microbiology...
Listeriolysin O (LLO) is a major virulence factor secreted by the pathogenic Listeria monocytogenes and acts as pore-forming cytolysin. Based on sequence similarities between LLO and perfringolysin (PFO), the cytolysin from Clostridium perfringens of known crystallographic structure, two truncated LLO proteins were produced: LLO-d123, comprising the first three predicted domains, and LLO-d4, the last C-terminal domain. The two proteins were efficiently secreted into the culture supernatant of L. monocytogenes and were able to bind to cell membranes. Strikingly, when expressed simultaneously, the two secreted domains LLO-d123 and LLO-d4 reassembled into a haemolytically active form. Two in-frame linker insertions were generated in the hinge region between the d123 and d4 domains. In both cases, the insertion created a major cleavage site for proteolytic degradation and abolished cytolytic activity, which might suggest that the region connecting d123 and d4 participates in the interaction between the two
Complete Genome Sequence of Listeria monocytogenes Strain DPC6895, a S by Aidan Casey, Aidan Coffey et al."Complete Genome Sequence of Listeria monocytogenes Strain DPC6895, a S" by Aidan Casey, Aidan Coffey et al.
Listeria monocytogenes is a foodborne pathogen and is the causative agent of listeriosis among humans and animals. The draft genome sequence of L. monocytogenes DPC6895, a serotype 1/2b strain isolated from the raw milk of a cow with subclinical bovine mastitis, is reported.
Louisiana Firm Recalls Frozen Chicken Breast Strips For Possible Listeria Monocytogenes ContaminationLouisiana Firm Recalls Frozen Chicken Breast Strips For Possible Listeria Monocytogenes Contamination
Foster Farms, a Farmerville, La., establishment, is recalling approximately 39,747 pounds of frozen pre-cooked chicken products due to possible contamination with Listeria monocytogenes.
TEL - Thèses en ligne - Characterization of Histone H3 Lysine 18 deacetylation during infection with Listeria monocytogenesTEL - Thèses en ligne - Characterization of Histone H3 Lysine 18 deacetylation during infection with Listeria monocytogenes
Bacterial pathogens dramatically affect host cell transcription programs for their own profit, however the underlying mechanism in most cases remain elusive. While investigating the effects of listeria monocytogenes on histone modifications, we discovered a new transcription regulatory machanism by which the expression of genes is repressed, during infection. Upon infection by L. monocytogenes, the secret virulence factor, InlB, binds the c-Met receptor and activates signaling through PI3K/Akt. This signaling platform is necessary for causing the relocalization of the histone deacetylase, SIRT2, to the nucleus and associating to chromatin.In characterizing the mechanism governing SIRT2 nuclear relocazing during infection, our results have demonstrated that SIRT2 undergoes a post-translational modification. SIRT2 undergoes dephosphorylation at a novel N-terminal phospho-site. SIRT2 is recruiter to the transcription star sites of genes repressed during inection leading to H3K18 deacetylation and
Impact of dilution ratios on Listeria monocytogenes growth during University of Vermont medium enrichment of deli meats. -...Impact of dilution ratios on Listeria monocytogenes growth during University of Vermont medium enrichment of deli meats. -...
In the U.S. Department of Agriculture (USDA) method for Listeria detection, a 25-g composite food sample is enriched in 225 ml of University of Vermont medium (UVM), giving a detection limit of 0.04 CFU/g. However, in a recent large-scale four-state deli meat survey for L. monocytogenes, 125-g samples enriched in 1,125 ml of UVM were requested to increase the detection limit to 0.008 CFU/g. To circumvent problems associated with large volumes of UVM, the impact on L. monocytogenes growth of lower dilution ratios used for enrichment and most-probable-number (MPN) detection was compared with the results obtained using the conventional 1:10 dilution. In this study, 125-g samples of cured turkey, uncured turkey, ham, and roast beef were inoculated with a six-strain L. monocytogenes cocktail to contain approximately 1 x 10(3) CFU/g. This cocktail was then diluted 1:3, 1:5, or 1:10 in UVM, homogenized, enriched at 30 degrees C, and periodically plated on modified Oxford agar to determine generation times
Antimicrobial activity of cetylpyridinium chloride against Listeria monocytogenes on frankfurters and subsequent effect on...Antimicrobial activity of cetylpyridinium chloride against Listeria monocytogenes on frankfurters and subsequent effect on...
Frankfurters inoculated with Listeria monocytogenes were treated with 1% cetylpyridinium chloride (CPC) or with 1% CPC followed by a water rinse at various combinations of spray temperatures (25, 40, and 558C), spray pressures (20, 25, and 35 psi), and times of exposure (30, 40, and 60 s). No significant differences (P , 0.05) were observed in the reductions achieved by 1% CPC + water wash and those achieved with 1% CPC treatment alone. L. monocytogenes populations were reduced by ca. 1.7 log CFU/g immediately following treatment, with no differences (P , 0.05) observed for different spray temperatures, pressures, or exposure times. The effectiveness of 1% CPC spray treatment (at 258C, 20 psi, and 30 s of exposure) against L. monocytogenes on vacuum-packaged frankfurters stored at 0 and 48C for 42 days was then evaluated. Application of a 1% CPC surface spray to frankfurters immediately prior to packaging reduced L. monocytogenes concentrations by 1.4 to 1.7 log CFU/g and further restricted ...
RASFF Alerts - Listeria monocytogenes -Smoked Salmon - Tahini - Raw Milk Cheese -Cooked Crab Meat | FoodWorldRASFF Alerts - Listeria monocytogenes -Smoked Salmon - Tahini - Raw Milk Cheese -Cooked Crab Meat | FoodWorld
RASFF - Listeria monocytogenes (980 CFU/g) in smoked salmon from Poland in France RASFF - Listeria monocytogenes (presence /25g) in tahini from Turkey, via Germany in the Netherlands RASFF - Listeria monocytogenes (1.7x10E5 CFU/g) in thawed sliced smoked salmon from Lithuania, packaged in Italy in Hungary RASFF - Listeria monocytogenes (180 CFU/g) in raw milk cheese from Spain in France…
Survival of Listeria monocytogenes, Listeria innocua, and Lactic acid bacteria species in chill brineSurvival of Listeria monocytogenes, Listeria innocua, and Lactic acid bacteria species in chill brine
Listeria monocytogenes is the major pathogen in ready-to-eat meat products such as deli meats and frankfurters. Contamination can occur via the salt brines that are used to cool thermally processed meats. Both L. monocytogenes and lactic acid bacteria can grow and thrive under these brine conditions, and may become competitive with each other for available nutrients. The objective of this study was to determine the effect of a three strain cocktail of lactic acid bacteria Enterococcus faecalis, Carnobacterium gallinarum, and Lactobacillus plantarum on the survival of Listeria monocytogenes and Listeria innocua in brines stored under low temperatures up to 10 days. Three brine concentrations (0%, 7.9%, and 13.2% NaCl) were inoculated with ~7.0 log10 cfu/ml of one of five cocktails (L. monocytogenes, L. innocua, lactic acid bacteria (LAB), L. monocytogenes + LAB, or L. innocua + LAB) and stored for 10 days at either 4°C or 12°C. Three replications of each brine/cocktail/temperature combination ...
Collapse of the proton motive force in Listeria monocytogenes caused by a bacteriocin produced by Pediococcus acidilactici<...Collapse of the proton motive force in Listeria monocytogenes caused by a bacteriocin produced by Pediococcus acidilactici<...
TY - JOUR. T1 - Collapse of the proton motive force in Listeria monocytogenes caused by a bacteriocin produced by Pediococcus acidilactici. AU - Christensen, D. P.. AU - Hutkins, R. W.. PY - 1992/1/1. Y1 - 1992/1/1. N2 - The effect of pediocin JD, a bacteriocin produced by Pediococcus acidilactici JD1-23, on the proton motive force and proton permeability of resting whole cells of Listeria monocytogenes Scott A was determined. Control cells, treated with trypsin-inactivated bacteriocin at a pH of 5.3 to 6.1, maintained a pH gradient and a membrane potential of approximately 0.65 pH unit and 75 mV, respectively. However, these gradients were rapidly dissipated in cells after exposure to pediocin JD, even though no cell lysis had occurred. The pH gradient and membrane potential of the producer cells were also unaffected by the bacteriocin. Whole cells treated with bacteriocin were twice as permeable to protons as control cells were. The results suggest that the inhibitory action of pediocin JD ...
DSpace ISS: Prevalence of Salmonella enterica and Listeria monocytogenes contamination in foods of animal origin in ItalyDSpace ISS: Prevalence of Salmonella enterica and Listeria monocytogenes contamination in foods of animal origin in Italy
BUSANI L, CIGLIANO A, TAIOLI E, CALIGIURI V, CHIAVACCI L, DI BELLA C, BATTISTI A, DURANTI A, GIANFRANCESCHI M, NARDELLA MC, RICCI A, ROLESU S, TAMBA M, MARABELLI R, CAPRIOLI A (2005) . Prevalence of Salmonella enterica and Listeria monocytogenes contamination in foods of animal origin in Italy. Journal of Food Protection 68 8 1729 - ...
Mycotic abdominal aortic aneurysm due to Listeria monocytogenes | Enfermedades Infecciosas y Microbiología Clínica (English...Mycotic abdominal aortic aneurysm due to Listeria monocytogenes | Enfermedades Infecciosas y Microbiología Clínica (English...
Listeria monocytogenes is a rare pathogen that principally affects neonates, pregnant women and immunosuppressed patients. It most commonly causes gastroenteritis in healthy adults and central nervous system infection in immunosuppressed patients. Cardiovascular infection due to Listeria monocytogenes is rare and tends to cause endocarditis.1 This article presents the case of a mycotic aortic aneurysm due to Listeria monocytogenes.. A 76-year-old man with a history of hypertension, type 2 diabetes mellitus, dyslipidaemia and ischaemic heart disease attended the emergency department following 2 months of general discomfort, hyporexia, low back pain and 7kg weight loss in the previous 2 weeks. The patient was afebrile and the physical examination revealed no significant findings. The vascular examination found distal pulses in both lower limbs. Bloods were normal, except for blood glucose at 460mg/dl. The chest and abdominal X-rays were normal. After normalising the patients blood glucose levels, ...
Meningitis neonatal por Listeria monocytogenes en San Luis, Argentina: análisis de tres casosMeningitis neonatal por Listeria monocytogenes en San Luis, Argentina: análisis de tres casos
Correspondence. E-mail: [email protected] ABSTRACT. Between November 1996 and December 2006, two cases of early-onset and one case of late-onset neonatal listeriosis were reported in San Luis, Argentina. This article describes clinical and laboratory findings as well as treatment and outcome for newborns treated for Listeria monocytogenes meningitis or septicaemia. In one of the newborns with early-onset listeriosis, meningitis led to important complications including hydrocephalus. The two other newborns showed complete recovery following adequate treatment. The L. monocytogenes isolates from two patients belonged to PCR group IVb (including serovar 4b strains) and to PCR group IIb (including serovar 1/2b strains) in the third patient. Listeriosis, especially the maternal-fetal presentation, is still rare in Argentina for unknown reasons. Our data can be used in the future as an epidemiological survey.. Key words: Listeria monocytogenes; Neonatal meningitis; Argentina. RESUMEN. Meningitis ...
Characterization of plasmids from Listeria monocytogenes and Listeria innocua strains isolated from short-ripened cheesesCharacterization of plasmids from Listeria monocytogenes and Listeria innocua strains isolated from short-ripened cheeses
The plasmid content of 30 isolates of Listeria monocytogenes and 18 isolates of Listeria innocua obtained from short-ripened cheeses was analysed. The isolates of L. monocytogenes serogroup 1 harboured a single plasmid, pLM33 (33.2 kbp), whereas the serogroup 4 isolates did not contain plasmids. One group of L. innocua strains harboured the plasmid pLI71 (71 kbp) and another one contained two plasmids: pLI59 (59.5 kbp) and pLI56 (56.5 kbp). These plasmid groups were in accordance with clusters previously defined by pulsed-field gel electrophoresis analysis of the chromosomal DNA of Listeria isolates. Plasmids pLM33, pLI71 and pLI59 shared homology regions of at least 20 kbp. Plasmid pLI56 did not encode genes for any known character (such as carbohydrate fermentation, resistance to antibiotics, heavy metals or disinfectants, growth at low pH, NaCl tolerance or thermal inactivation by pasteurisation) and displayed different characteristics to the other three plasmids. It was also the only one ...
Effect of natural antimicrobials against Salmonella, Escherichia coli o157:h7 and Listeria monocytogenesEffect of natural antimicrobials against Salmonella, Escherichia coli o157:h7 and Listeria monocytogenes
Salmonella, Escherichia coli O157:H7 and Listeria monocytogenes are pathogens that have caught the attention of federal agencies and researchers due to their great economic impact when illnesses occur. To reduce the presence of these pathogens, different approaches have been used. However, since the global consumers demand for natural ingredients is steadily increasing, the investigation of the effectiveness of potential natural antimicrobials is necessary. In this study, the in vitro antimicrobial activity of Hibiscus sabdariffa L extracts against Salmonella, E. coli O157:H7 and L. monocytogenes was investigated. Furthermore, H. sabdariffa L and ε-polylysine were evaluated to reduce populations of Salmonella and E. coli O157:H7 in ground beef. The minimum inhibitory concentration (MIC) of H. sabdariffa L extracts against Salmonella and E. coli O157:H7 was 6,489 μg/mL and for L. monocytogenes, 5,309 μg/mL. The minimum bactericidal concentration (MBC) of H. sabdariffa L extracts against ...
Molecular Typing and Epidemiology of Human Listeriosis Cases, Denmark, 2002-20121 - Volume 22, Number 4-April 2016 - Emerging...Molecular Typing and Epidemiology of Human Listeriosis Cases, Denmark, 2002-20121 - Volume 22, Number 4-April 2016 - Emerging...
Denmark has a high incidence of invasive listeriosis (0.9 cases/100,000 population in 2012). We analyzed patient data, clinical outcome, and trends in pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing (MLST) of Listeria monocytogenes strains isolated in Denmark during 2002-2012. We performed 2-enzyme PFGE and serotyping on 559 isolates and MLST on 92 isolates and identified some correlation between molecular type and clinical outcome and patient characteristics. We found 178 different PFGE types, but isolates from 122 cases belonged to just 2 closely related PFGE types, clonal complex 8 and sequence type 8. These 2 types were the main cause of a peak in incidence of invasive listeriosis during 2005-2009, possibly representing an outbreak or the presence of a highly prevalent clone. However, current typing methods could not fully confirm these possibilities, highlighting the need for more refined discriminatory typing methods to identify outbreaks within frequently occurring L.
Effects of Sodium Lactate on the Survival of Listeria Monocytogenes, Escherichia coli O157:H7, and Salmonella spp. in Cooked...Effects of Sodium Lactate on the Survival of Listeria Monocytogenes, Escherichia coli O157:H7, and Salmonella spp. in Cooked...
The objective of this study was to determine the effect of sodium lactate on the survival of Listeria monocytogenes, Escherichia coli O157: H7, and Salmonella spp. in cooked ham during storage at refrigerated and abuse temperatures. Cooked ham was added with 0% - 3% lactate, inoculated with a multiple-strain mixture of L. monocytogenes, E. coli O157: H7, or Salmonella spp. and stored at 4oC - 15oC for up to 35 day. The growth of the three pathogens was inhibited in ham containing 3% lactate, and no growth of E. coli O157: H7 and Salmonella spp. occurred at the lowest storage tem- peratures of 6 and 8oC, respectively. In ham containing no lactate, the average growth rates were 0.256 - 0.380 log CFU/day for L. monocytogenes at 4oC - 8oC, 0.242 - 0.315 log CFU/day for E. coli O157: H7 at 8oC - 15oC, and 0.249 - 0.328 log CFU/day for Salmonella spp. at 10oC - 15oC. The addition of 1% or 2% lactate significantly (P | 0.05) reduced the growth rates of the three pathogens, and the effect was more profound at
Prevalence and antimicrobial susceptibility profile of listeria species from ready-to-eat foods of animal origin in Gondar Town...Prevalence and antimicrobial susceptibility profile of listeria species from ready-to-eat foods of animal origin in Gondar Town...
Listeriosis, mostly caused by Listeria monocytogenes species, has become a major concern to public health authorities due to its clinical severity and high mortality rate, particularly in high risk groups. Currently, there is limited information regarding the prevalence and antimicrobial susceptibility profiles of listeria species in ready-to-eat foods of animal origin in Gondar town, Ethiopia. The aim of this study was to determine the prevalence and antimicrobial susceptibility pattern of Listeria species isolated from ready-to-eat food of animal origin from public dinning places in Gondar town, Ethiopia. A cross sectional study on ready-toeat foods of animal origin sampled from major supermarkets, butcher shops, pastry shops, restaurants and hotels was carried out. Culture, biochemical and sugar tests were conducted for listeria species identification and disc diffusion test was performed to study the antimicrobial susceptibility profiles of the isolates. Out of 384 food samples examined, 96 (25%)
Listeria Monocytogenes Archives | Page 2 of 61 | Food Poisoning BulletinListeria Monocytogenes Archives | Page 2 of 61 | Food Poisoning Bulletin
The Little Milk Company in Ireland is recalling a batch of Organic Irish Cheddar Cheese for possible Listeria monocytogenes contamination, according to a notice posted on the Food Safety Authority of Ireland website. We are telling you about this because this dairy does sell some cheeses in New York City in the United States according to information on its website; we just dont know exactly where. In Ireland, the recalled cheeses include Organic Mild Cheddar sold in 135 gram packages; Milk Organic Wedge in 1 kg packages, and Grated Cheese in 2 kg packages. The batch number is B82. The best before dates on these cheeses are: 18/12/2017, 01/01/2018, 14/01/2018, 25/01/2018, 12/02/2018, 05/03/2018, 12/03/2018, 26/03/2018, 17/07/2018. The sizes, batch number, and best before dates on cheese … [Read more...] ...
Listeria in Lettuce: How much protection is enough? | GotFoodPoisoning.comListeria in Lettuce: How much protection is enough? | GotFoodPoisoning.com
Listeria monocytogenes: How much protection is enough?. By: Ryan Robinson, PhD. Recent studies involving Listeria monocytogenes found in lettuce is causing concern for food safety. In foods such as lettuce, which are not heated prior to eating, there is a concern as to how safe ready-to-eat foods can be, even with precautionary action. Is washing the lettuce enough? How much do we need to do to ensure we are doing what we can to prevent ourselves, and those we care about, from becoming sick? When is enough, well, enough?. Listeria monocytogenes, most frequently referred to as simply "Listeria", is a pathogenic, rod-shaped bacterial organism. It is commonly associated with foodborne illnesses originating from consumption of tainted deli meat, hot dogs, and unpasteurized dairy, but may also be associated with contaminated produce.. While outbreaks of Listeria are less common than other foodborne illnesses (e.g. Salmonella or E. coli infections) they are incredibly dangerous. Several studies ...
Comparative Analysis of Plasmids in the Genus ListeriaComparative Analysis of Plasmids in the Genus Listeria
Background: We sequenced four plasmids of the genus Listeria, including two novel plasmids from L. monocytogenes serotype 1/2c and 7 strains as well as one from the species L. grayi. A comparative analysis in conjunction with 10 published Listeria plasmids revealed a common evolutionary background. Principal Findings: All analysed plasmids share a common replicon-type related to theta-replicating plasmid pAMbeta1. Nonetheless plasmids could be broadly divided into two distinct groups based on replicon diversity and the genetic content of the respective plasmid groups. Listeria plasmids are characterized by the presence of a large number of diverse mobile genetic elements and a commonly occurring translesion DNA polymerase both of which have probably contributed to the evolution of these plasmids. We detected small non-coding RNAs on some plasmids that were homologous to those present on the chromosome of L. monocytogenes EGD-e. Multiple genes involved in heavy metal resistance (cadmium, copper, ...
Listeria monocytogenes Flagella Are Used for Motility, Not as Adhesins, To Increase Host Cell Invasion | Infection and ImmunityListeria monocytogenes Flagella Are Used for Motility, Not as Adhesins, To Increase Host Cell Invasion | Infection and Immunity
Flagella and flagellum-mediated motility are integral to the virulence of several gastrointestinal bacterial pathogens (10). For L. monocytogenes, no link has been made between flagella and virulence, although the flagella are important for efficient invasion of tissue culture cells (2, 6). In this study, we investigated the mechanism by which flagella influence the ability of L. monocytogenes to invade host cells and the role of flagella in colonizing mice early in infection. Our results clearly indicate that L. monocytogenes flagella do not function as adhesins to enhance bacterial attachment to and invasion of epithelial cells, but rather function as motility devices contributing more to invasion than a mere increase in probability of bacterium-host cell interaction. Moreover, we show that motile bacteria outcompete nonmotile bacteria for initial colonization of the intestinal tract and liver by L. monocytogenes.. Flagella can function as adhesins, independent of motility, to enhance ...
SWISSPROT: Y1020 LISMHSWISSPROT: Y1020 LISMH
ID Y1020_LISMH Reviewed; 224 AA. AC B8DHJ9; DT 28-JUL-2009, integrated into UniProtKB/Swiss-Prot. DT 03-MAR-2009, sequence version 1. DT 11-DEC-2019, entry version 54. DE RecName: Full=UPF0758 protein LMHCC_1020; GN OrderedLocusNames=LMHCC_1020; OS Listeria monocytogenes serotype 4a (strain HCC23). OC Bacteria; Firmicutes; Bacilli; Bacillales; Listeriaceae; Listeria. OX NCBI_TaxID=552536; RN [1] RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=HCC23; RX PubMed=21602330; DOI=10.1128/jb.05236-11; RA Steele C.L., Donaldson J.R., Paul D., Banes M.M., Arick T., Bridges S.M., RA Lawrence M.L.; RT "Genome sequence of lineage III Listeria monocytogenes strain HCC23."; RL J. Bacteriol. 193:3679-3680(2011). CC -!- SIMILARITY: Belongs to the UPF0758 family. {ECO:0000305}. DR EMBL; CP001175; ACK39368.1; -; Genomic_DNA. DR RefSeq; WP_012581272.1; NC_011660.1. DR SMR; B8DHJ9; -. DR EnsemblBacteria; ACK39368; ACK39368; LMHCC_1020. DR KEGG; lmh:LMHCC_1020; -. DR HOGENOM; HOG000273374; -. DR KO; ...
Route of Infection Determines the Impact of Type I Interferons on Innate Immunity to Listeria monocytogenes - pdf descargarRoute of Infection Determines the Impact of Type I Interferons on Innate Immunity to Listeria monocytogenes - pdf descargar
Route of Infection Determines the Impact of Type I Interferons on Innate Immunity to Listeria monocytogenes. . Biblioteca virtual para leer y descargar libros, documentos, trabajos y tesis universitarias en PDF. Material universiario, documentación y tareas realizadas por universitarios en nuestra biblioteca. Para descargar gratis y para leer online.
Certain Ballarini brand Gorgonzola cheese may contain Listeria monocytogenes - Health Hazard Alert - Canadian Food Inspection...Certain Ballarini brand Gorgonzola cheese may contain Listeria monocytogenes - Health Hazard Alert - Canadian Food Inspection...
The Canadian Food Inspection Agency (CFIA) and Jan K Overweel Ltd. are warning the public not to consume the Ballarini brand Gorgonzola cheese products described below because the products may be contaminated with Listeria monocytogenes.
Bee Healthy Farms: Mexican Honeys Prove Antioxidant, Antibacterial ActionBee Healthy Farms: Mexican Honeys Prove Antioxidant, Antibacterial Action
A total of 14 Mexican honeys were screened for quality parameters including color, moisture, proline, and acidity. Antioxidant properties of complete honey and its methanolic extracts were evaluated by the DPPH, ABTS, and FRAP assays. In addition, the antimicrobial activity of complete honeys against Bacillus cereus ATCC 10876, Listeria monocytogenes Scott A, Salmonella Typhimurium ATCC 14028, and Sthapylococcus aureus ATCC 6538 was determined ...
Listeria Infection Medication: AntibioticsListeria Infection Medication: Antibiotics
Listeriosis is an infection caused by the gram-positive motile bacterium Listeria monocytogenes. Listeriosis is relatively rare and occurs primarily in newborn infants, elderly patients, and patients who are immunocompromised.
Listeria Infection Differential DiagnosesListeria Infection Differential Diagnoses
Listeriosis is an infection caused by the gram-positive motile bacterium Listeria monocytogenes. Listeriosis is relatively rare and occurs primarily in newborn infants, elderly patients, and patients who are immunocompromised.
Leicester Research Archive: A numerical taxonomic and serological study of Listeria and possibly related bacteria.Leicester Research Archive: A numerical taxonomic and serological study of Listeria and possibly related bacteria.
A numerical taxonomic survey has heen performed using 192 strains of Listeria and possibly related bacteria. Of these, 38 strains have heen further investigated hy serological techniques. The results indicate that Listeria monocytogenes is a good, homogeneous species, L. grayi and L, murrayi do not differ sufficiently from each other to warrant individual specific status, and it is recommended that they he combined in the single species L, grayi. They show a close relationship to L. monocytogenes, compatible with that of two species within the same genus, but L. denitrificans differs greatly from the other Listeria spp, and should be removed from the genus, Erysipelothrix, Listeria and Microbacterium thermosphac- tum show a closer resemblance to the family Lactobacillaceae than to the family Corynebacteriaceae where they are at present classified. The suggested relationship of Gemella haemol- ysans to the tribe Streptococceae in the Lactobacillaceae has been confirmed, and it is possible to ...
purM - Phosphoribosylformylglycinamidine cyclo-ligase - Listeria monocytogenes serovar 1/2a (strain ATCC BAA-679 / EGD-e) -...purM - Phosphoribosylformylglycinamidine cyclo-ligase - Listeria monocytogenes serovar 1/2a (strain ATCC BAA-679 / EGD-e) -...
p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.,/p> ,p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.,/p> ,p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).,/p> ,p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x,sup>64,/sup> + x,sup>4,/sup> + x,sup>3,/sup> + x + 1. The algorithm is described in the ISO 3309 standard. ,/p> ,p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.,br /> ,strong>Cyclic redundancy and other checksums,/strong>,br /> ,a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993),/a>),/p> Checksum:i ...
Birdseye Peas recalled due to possible Listeria contamination | CommissariesBirdseye Peas recalled due to possible Listeria contamination | Commissaries
Pinnacle Foods Inc. is voluntarily recalling a limited number of Birds Eye Baby Sweet Peas after testing indicated the presence of Listeria monocytogenes in the retail environment, according to a Department of Defense All Food and Drug Activity message sent Sept. 30.. The Defense Commissary Agency has publicized this recall to all its stores, said Richard Stith, lead consumer safety officer for DeCA headquarters at Fort Lee, Virginia. Whenever a commissary has a recalled or withdrawn product in its inventory, the product is immediately removed from store shelves.. The following product is affected by this recall:. • Birds Eye Baby Sweet Peas, 13 ounces: UPC 014500022530 with "Best By" dates of July 5 and 6, 2019.. Listeria monocytogenes may cause serious and sometimes fatal infections in young children, the frail or elderly, and those with weakened immune systems. Healthy adults may only suffer short-term symptoms such as high fever, severe headache, stiffness, nausea, abdominal pain and ...
HOGENOM: LISMC 1 PE1001HOGENOM: LISMC 1 PE1001
ID LISMC_1_PE1001 STANDARD; PRT; 212 AA. AC LISMC_1_PE1001; C1L1U3; DT 00-JAN-0000 (Rel. 1, Created) DT 00-JAN-0000 (Rel. 2, Last sequence update) DT 00-JAN-0000 (Rel. 3, Last annotation update) DE SubName: Full=Putative two-component response regulator; DE (LISMC_1.PE1001). GN OrderedLocusNames=Lm4b_01042; OS LISTERIA MONOCYTOGENES SEROTYPE 4B STR. CLIP 80459. OC Bacteria; Firmicutes; Bacillales; Listeriaceae; Listeria. OX NCBI_TaxID=568819; RN [0] RP -.; RG -.; RL -.; CC -!- SEQ. DATA ORIGIN: Translated from the HOGENOM CDS LISMC_1.PE1001. CC Listeria monocytogenes Clip81459, complete genome. CC complete sequence. CC -!- ANNOTATIONS ORIGIN:C1L1U3_LISMC CC -!- SIMILARITY: Contains 1 HTH luxR-type DNA-binding domain. CC -!- SIMILARITY: Contains 1 response regulatory domain. CC -!- GENE_FAMILY: HOG000034813 [ FAMILY / ALN / TREE ] DR UniProtKB/Swiss-Prot; C1L1U3; -. DR EMBL; FM242711; CAS04808.1; -; Genomic_DNA. DR RefSeq; YP_002757747.1; NC_012488.1. DR ProteinModelPortal; C1L1U3; -. DR SMR; ...
Listeria : Food Safety WatchListeria : Food Safety Watch
LISTERIA. What is Listeria? Listeria is a genus of Gram-positive, rod-shaped bacteria, containing a number of species including L. monocytogenes, L. innocua, L. welshimeri, L. seeligeri, L. ivanovii and L. grayi. Although the first four of these have all been implicated in human infection nearly all cases of Listeria infection are caused by L. monocytogenes. At […]. ...
Browsing Food Programme by TitleBrowsing Food Programme by Title
Due to its ubiquitous nature, Listeria monocytogenes is a threat to all fresh fruits and vegetables, including mushrooms, which are Irelands largest horticultural crop. Although fresh cultivated mushrooms (Agaricus bisporus) have not been previously linked with listeriosis outbreaks, the pathogen still poses a threat to the industry, particularly due to its ability to form biofilms. This threat is highlighted by the multiple recalls of mushroom products caused by L. monocytogenes contamination and by previous studies demonstrating that L. monocytogenes is present in the mushroom production environment. In this study, the biofilm formation potential of L. monocytogenes strains isolated from the mushroom production environment was investigated on materials and at temperatures relevant to mushroom production. A preliminary assessment of biofilm formation of 73 mushroom industry isolates was undertaken using a crystal violet assay on polystyrene microtitre plates. The biofilm formation of a subset ...
KEGG PATHWAY: Biotin metabolism - Listeria monocytogenes ATCC 19117KEGG PATHWAY: Biotin metabolism - Listeria monocytogenes ATCC 19117
Biotin (vitamin H or vitamin B7) is the essential cofactor of biotin-dependent carboxylases, such as pyruvate carboxylase and acetyl-CoA carboxylase. Mammals cannot synthesize biotin, while in bacteria, fungi, and plants it is synthesized from pimelate thioester through different pathways. In E. coli and many organisms, pimelate thioester is derived from malonyl-ACP. The pathway starts with the methylation to malonyl-ACP methyl ester, followed by the fatty acid chain elongation cycle to form pimeloyl-ACP methyl ester, which is then demethylated to form pimeloyl-ACP [MD:M00572]. Pimeloyl-ACP is converted to biotin through the final four steps in the biotin bicyclic ring assembly, which are conserved among biotin-producing organisms [MD:M00123]. In B. subtilis, biotin is derived from pimeloyl-ACP formed by oxidative cleavage of long-chain acyl-ACPs [MD:M00573]. Some bacteria synthesize biotin from pimeloyl-CoA derived from pimelate [MD:M00577]. Biotin is covalently attached to biotin-dependent ...
Pinnacle Foods Inc. Recall - Pawnee Valley Community HopsitalPinnacle Foods Inc. Recall - Pawnee Valley Community Hopsital
On May 12, 2017, Pawnee Valley Community Hospital (PVCH) was notified by the Kansas Department of Health and Environment (KDHE) that a recall had been issued by Pinnacle Foods Inc. of some Aunt Jemima frozen breakfast foods including pancakes, waffles, and French toast slices, because they are potentially contaminated with Listeria monocytogenes. This recall was initiated after Listeria monocytogenes was found in the production environment. All affected distributors, retail, and food service customers are being notified. It was determined on May 9, 2017, that PVCH had served this brand of French toast in its cafeteria. The purpose of this letter is to inform PVCH patients and visitors that if you consumed this product at the PVCH facility on May 9, 2017, and if you develop any symptoms of listeriosis within 70 days of that date, please promptly notify your physician.. Listeria monocytogenes is an organism which can cause serious and sometimes fatal infections in young children, frail or elderly ...
Listeria</em> contamination causes Delifish smoked salmon recall -...Listeria</em> contamination causes Delifish smoked salmon recall -...
January 4, 2013 Delifish S.A. has issued a voluntary recall of cold smoked salmon products potentially containing Listeria monocytogenes placed on the market in the United States produced from lots 249-291, manufactured between September 5 and October 17, 2012. There have been no complaints or illnesses reported.. Product was possibly distributed in California, Virginia, Ohio, Illinois, Florida, Georgia, Texas, Delaware, New Jersey, New York, Pennsylvania, and Puerto Rico and reached consumers through retail stores under various brand names. The voluntary recall is carried out as a precautionary measure in cooperation with the U.S. Food and Drug Administration (FDA) and is based on Listeria detection in a few of the recalled batches. No other products supplied by Delifish or Marine Harvest USA are involved in this case. The FDA has a zero tolerance for Listeria in products intended to be eaten without prior heat treatment. In the European Union, the tolerance limit for such products is maximum ...
Listeriosis - NHSListeriosis - NHS
Listeriosis is an infection that usually develops after eating food thats been contaminated by listeria bacteria (Listeria monocytogenes)
California Apple Plants Deadly Listeria Outbreak | HealthMapCalifornia Apple Plant's Deadly Listeria Outbreak | HealthMap
In the middle of December 2014, the CDC announced an outbreak of Listeria linked to commercially produced caramel apples. The outbreak resulted in 32 individuals being hospitalized (FSN, CNN). One-third of those hospitalized were pregnant women, who are one of the high-risk groups of listeriosis (CNN). While seven deaths have been reported, the CDC has linked listeriosis to contributing to at least three of the deaths (CNN). The first illnesses were reported in mid-October -- the peak of caramel apple season -- and the outbreak has spread to 11 states (AZ, CA, MN, MO, NM, NC, NV, TX, UT, WA, WI) (FSN). Two cases of listeriosis have been reported in Canada, with the same PFGE ("DNA Fingerprint") as the US strains. The majority of the ill people interviewed (89%) reported eating commercially produced and prepackaged caramel apples prior to their illness onset (CDC).. Listeria: The Bug. Listeria monocytogenes is a bacterium that can cause serious illness: Listeriosis. Listeriosis generally results ...
Frontiers | LMOf2365 0442 Encoding for a Fructose Specific PTS Permease IIA May Be Required for Virulence in L. monocytogenes...Frontiers | LMOf2365 0442 Encoding for a Fructose Specific PTS Permease IIA May Be Required for Virulence in L. monocytogenes...
Listeria monocytogenes is a foodborne pathogen that causes listeriosis, which is a major public health concern due to the high fatality rate. LMOf2365_0442, 0443, and 0444 encode for fructose-specific EIIABC components of phosphotransferase transport system (PTS) permease that is responsible for sugar transport. In previous studies, in-frame deletion mutants of a putative fructose-specific PTS permease (LMOf2365_0442, 0443, and 0444) were constructed and analyzed. However, the virulence potential of these deletion mutants has not been studied. In this study, two in vitro methods were used to analyze the virulence potential of these L. monocytogenes deletion mutants. First, invasion assays were used to measure the invasion efficiencies to host cells using the human HT-29 cell line. Second, plaque forming assays were used to measure cell-to-cell spread in host cells. Our results showed that the deletion mutant ΔLMOf2365_0442 had reduced invasion and cell-to-cell spread efficiencies in human cell line
Kashi Company recalls Granola Bars due to possible Listeria contamination | CommissariesKashi Company recalls Granola Bars due to possible Listeria contamination | Commissaries
Listeria monocytogenes may cause serious and sometimes fatal infections in young children, the frail or elderly, and those with weakened immune systems. Healthy adults may only suffer short-term symptoms such as high fever, severe headache, stiffness, nausea, abdominal pain and diarrhea. Listeria infections may also cause miscarriages and stillbirths among pregnant women. No illnesses have been reported to date, but the illness caused by these bacteria can take up to 70 days to appear.. Customers should return this product to the commissary of purchase for a full refund.. For additional information and a full list of products affected by this recall please visit the Food and Drug Administration website.. Questions about this recall can be directed to the Consumer Affairs Hotline at 1-877-747-2467, Monday through Friday, 9 A.M. to 6 P.M. ET.. ...
Ashe County Cheese Company Recalls Mexican Style Quesco Fresco CheeseAshe County Cheese Company Recalls Mexican Style Quesco Fresco Cheese
SafetyAlerts) - Ashe County Cheese Company of West Jefferson, North Carolina, a division of Newburg Corners Cheese, Inc., is voluntarily recalling 237 cases, (2133 lbs.), of Mexican style Quesco Fresco cheese because it may contain the bacteria Listeria Monocytogenes; an organism that can cause serious and sometimes fatal infections in young children, frail or elderly people, an others with impaired immune systems. Although healthy individuals may suffer only short term symptoms such as headaches, stiffness, nausea, abdominal pain, and diarrhea, Listeria infection can cause miscarriages and stillbirths among pregnant women ...
Pathogenesis of listeria-infected Drosophila wntD mutants is associated with elevated levels of the novel immunity gene edin. |...Pathogenesis of listeria-infected Drosophila wntD mutants is associated with elevated levels of the novel immunity gene edin. |...
Drosophila melanogaster mount an effective innate immune response against invading microorganisms, but can eventually succumb to persistent pathogenic infections. Understanding of this pathogenesis is limited, but it appears that host factors, induced by microbes, can have a direct cost to the host organism. Mutations in wntD cause susceptibility to Listeria monocytogenes infection, apparently through the derepression of Toll-Dorsal target genes, some of which are deleterious to survival. Here, we use gene expression profiling to identify genes that may mediate the observed susceptibility of wntD mutants to lethal infection. These genes include the TNF family member eiger and the novel immunity gene edin (elevated during infection; synonym CG32185), both of which are more strongly induced by infection of wntD mutants compared to controls. edin is also expressed more highly during infection of wild-type flies with wild-type Salmonella typhimurium than with a less pathogenic mutant strain, and its ...
HypothiocyaniteHypothiocyanite
MesophileMesophile
List of foodborne illness outbreaks by death tollList of foodborne illness outbreaks by death toll
Dakota StyleDakota Style
1985 California listeriosis outbreak in cheese1985 California listeriosis outbreak in cheese
PrfA thermoregulator UTRPrfA thermoregulator UTR
Organic acidOrganic acid
Neonatal meningitisNeonatal meningitis
PhagosomePhagosome
Sereny testSereny test
InternalinInternalin
Multilocus sequence typingMultilocus sequence typing
Lipoyl amidotransferaseLipoyl amidotransferase
StreptococcusStreptococcus
Hemolysis (microbiology)Hemolysis (microbiology)
Actin assembly-inducing proteinActin assembly-inducing protein
ChemotaxisChemotaxis
International Facility for Food Irradiation TechnologyInternational Facility for Food Irradiation Technology
Geotrichum candidumGeotrichum candidum
Listeriolysin OListeriolysin O
2008 Canada listeriosis outbreak2008 Canada listeriosis outbreak
2011 United States listeriosis outbreak2011 United States listeriosis outbreak
Sortase BSortase B
Health crisisHealth crisis
NOD-like receptorNOD-like receptor
RNA thermometerRNA thermometer
MeropenemMeropenem
Host-directed therapeuticsHost-directed therapeutics
FourU thermometerFourU thermometer
PTS Galactitol FamilyPTS Galactitol Family
Streptococcus pneumoniaeStreptococcus pneumoniae
Staphylococcal scalded skin syndromeStaphylococcal scalded skin syndrome
Urinary tract infectionUrinary tract infection
Innate immune systemInnate immune system
SpheroplastSpheroplast
Intracellular parasiteIntracellular parasite
HemolysinHemolysin
CDH1 (gene)CDH1 (gene)
C1QBPC1QBP
TetanusTetanus
Kolektin - WikipedieKolektin - Wikipedie
RheniumRhenium
StreptococcusStreptococcus
Officina lactaria - VicipaediaOfficina lactaria - Vicipaedia
Porenbildendes ToxinPorenbildendes Toxin
BacillusBacillus
MeningitisMeningitis
PhagosomePhagosome
அகநச்சு - தமிழ் விக்கிப்பீடியாஅகநச்சு - தமிழ் விக்கிப்பீடியா
NeutrophilNeutrophil
RenijRenij
Public health genomicsPublic health genomics
Gas gangreneGas gangrene
PenicillinPenicillin
BacteriophageBacteriophage
Ureaplasma infectionUreaplasma infection
MilkMilk
Brain abscessBrain abscess
AnatomyOrganismsDiseasesChemicals and DrugsAnalytical, Diagnostic and Therapeutic Techniques and EquipmentPhenomena and ProcessesDisciplines and OccupationsTechnology, Industry, AgricultureInformation ScienceHealth Care
Listeria monocytogenesListeriosisListeriaMeningitis, ListeriaFood MicrobiologyHemolysin ProteinsBacterial ProteinsVirulencePeptide Termination FactorsCheeseHeat-Shock ProteinsBacterial ToxinsColony Count, MicrobialNisinMeat ProductsFood-Processing IndustryMacrophagesSpleenBacteriocinsGene Expression Regulation, BacterialFood PreservationFood ContaminationFoodborne DiseasesDNA, BacterialMice, Inbred C57BLMolecular Sequence DataMice, Inbred BALB CFood PreservativesMicrobial ViabilityImmunity, InnateVirulence FactorsCulture MediaSerotypingFood HandlingMilkPhagosomesGenes, BacterialImmunity, CellularLethal Dose 50Caco-2 CellsPhagocytosisBacterial AdhesionBenzalkonium CompoundsInterferon-gammaAntigens, BacterialEnvironmental MicrobiologyAnti-Bacterial AgentsCD8-Positive T-LymphocytesSigma FactorBacterial VaccinesImmunity, ActiveMacrophage ActivationBetaineLiverHemolysin FactorsAmpicillinBase SequenceHypersensitivity, DelayedPediococcusPhosphoinositide Phospholipase CMembrane ProteinsBacterial Typing TechniquesGene DeletionHot TemperatureRefrigerationMutationBacterial LoadCold TemperatureMice, KnockoutStainless SteelAmino Acid SequenceHemolysisTemperatureSequence Analysis, DNAImmunologic MemoryAlanine RacemaseAcidsMeatSpecies SpecificityRibotypingImmunization, PassiveCell WallCell LinePeritoneal CavityElectrophoresis, Gel, Pulsed-FieldHydrogen-Ion Concentration
Listeria monocytogenes exploits efferocytosis to promote cell-to-cell spread | NatureListeria monocytogenes exploits efferocytosis to promote cell-to-cell spread | Nature
Listeria monocytogenes virulence and pathogenicity, a food safety perspectiveListeria monocytogenes virulence and pathogenicity, a food safety perspective
Louisiana Firm Recalls Frozen Chicken Breast Strips For Possible Listeria Monocytogenes ContaminationLouisiana Firm Recalls Frozen Chicken Breast Strips For Possible Listeria Monocytogenes Contamination
Listeria monocytogenes (Murray et al.) Pirie ATCC ® 35152™Listeria monocytogenes (Murray et al.) Pirie ATCC ® 35152™
Listeria monocytogenes (Murray et al.) Pirie ATCC ® 19115™Listeria monocytogenes (Murray et al.) Pirie ATCC ® 19115™
Listeria Monocytogenes: Incidence, Growth Behavior and Control - Nova Science PublishersListeria Monocytogenes: Incidence, Growth Behavior and Control - Nova Science Publishers
Toxins | Free Full-Text | Distinct Neurotoxicity Profile of Listeriolysin O from Listeria monocytogenesToxins | Free Full-Text | Distinct Neurotoxicity Profile of Listeriolysin O from Listeria monocytogenes
Listeria monocytogenes σB Regulates Stress Response and Virulence Functions | Journal of BacteriologyListeria monocytogenes σB Regulates Stress Response and Virulence Functions | Journal of Bacteriology
Compliments brand Broccoli Slaw recalled due to Listeria monocytogenes - Food Recall Warning - Canadian Food Inspection AgencyCompliments brand Broccoli Slaw recalled due to Listeria monocytogenes - Food Recall Warning - Canadian Food Inspection Agency
Struktur und Funktion der 20S Proteasomen aus Organen Listeria monocytogenes infizierter MäuseStruktur und Funktion der 20S Proteasomen aus Organen Listeria monocytogenes infizierter Mäuse
TEL - Thèses en ligne - Characterization of Histone H3 Lysine 18 deacetylation during infection with Listeria monocytogenesTEL - Thèses en ligne - Characterization of Histone H3 Lysine 18 deacetylation during infection with Listeria monocytogenes
British Library EThOS: The role of complement properdin in murine infection with Listeria monocytogenesBritish Library EThOS: The role of complement properdin in murine infection with Listeria monocytogenes
Listeria monocytogenes - WikipediaListeria monocytogenes - Wikipedia
Listeria monocytogenes non-coding RNA - WikipediaListeria monocytogenes non-coding RNA - Wikipedia
Listeria monocytogenes | bacterium | Britannica.comListeria monocytogenes | bacterium | Britannica.com
Neogens ANSR® for Listeria monocytogenes receives AOAC approvalNeogen's ANSR® for Listeria monocytogenes receives AOAC approval
Listeria Monocytogenes Meningitis After Prostatectomy | The BMJListeria Monocytogenes Meningitis After Prostatectomy | The BMJ
lmo2271 lmo2271 [Listeria monocytogenes EGD-e] - Gene - NCBIlmo2271 lmo2271 [Listeria monocytogenes EGD-e] - Gene - NCBI
Listeria monocytogenes serotype 4bListeria monocytogenes serotype 4b
Listeria monocytogenes ATCC 19117Listeria monocytogenes ATCC 19117
Listeria Monocytogenes - Methods and Protocols | Edward M. Fox | SpringerListeria Monocytogenes - Methods and Protocols | Edward M. Fox | Springer
Listeria Monocytogenes Infection (Listeriosis): Background, Pathophysiology, EpidemiologyListeria Monocytogenes Infection (Listeriosis): Background, Pathophysiology, Epidemiology
What Do We Do about Listeria monocytogenes?What Do We Do about Listeria monocytogenes?
Listeria monocytogenes | MSDSonlineListeria monocytogenes | MSDSonline
Host cell signal transduction during Listeria monocytogenes infection. - PubMed - NCBIHost cell signal transduction during Listeria monocytogenes infection. - PubMed - NCBI
Federal Register :: Draft Interagency Risk Assessment-Listeria monocytogenesFederal Register :: Draft Interagency Risk Assessment-Listeria monocytogenes
Listeria Monocytogenes | Cornell University College of Veterinary MedicineListeria Monocytogenes | Cornell University College of Veterinary Medicine
Listeria monocytogenes | Journal of Clinical MicrobiologyListeria monocytogenes | Journal of Clinical Microbiology
Outbreak Investigation of Listeria monocytogenes: Hard-Boiled Eggs (December 2019) | FDAOutbreak Investigation of Listeria monocytogenes: Hard-Boiled Eggs (December 2019) | FDA
Map of Reported Cases | Multistate Outbreak of Listeria Monocytogenes Infections | August 2019 | Listeria | CDCMap of Reported Cases | Multistate Outbreak of Listeria Monocytogenes Infections | August 2019 | Listeria | CDC
Outbreak of Listeria Infections | Multistate Outbreak of Listeria Monocytogenes Infections | August 2019 | Listeria | CDCOutbreak of Listeria Infections | Multistate Outbreak of Listeria Monocytogenes Infections | August 2019 | Listeria | CDC
JCI - CXCR3 blockade protects against Listeria monocytogenes infection-induced fetal wastageJCI - CXCR3 blockade protects against Listeria monocytogenes infection-induced fetal wastage
Surface proteins and the pathogenic potential of Listeria monocytogenesSurface proteins and the pathogenic potential of Listeria monocytogenes
Advances in Listeria monocytogenes Infection Biology | Frontiers Research TopicAdvances in Listeria monocytogenes Infection Biology | Frontiers Research Topic
The proteolytic activity of Listeria monocytogenes HtrAThe proteolytic activity of Listeria monocytogenes HtrA
KEGG PATHWAY: Glycolysis / Gluconeogenesis - Listeria monocytogenes SLCC2755KEGG PATHWAY: Glycolysis / Gluconeogenesis - Listeria monocytogenes SLCC2755
Prevalencia de Listeria monocytogenes en queso IdiazabalPrevalencia de Listeria monocytogenes en queso Idiazabal
AnatomyOrganismsDiseasesChemicals and DrugsAnalytical, Diagnostic and Therapeutic Techniques and EquipmentPhenomena and ProcessesDisciplines and OccupationsTechnology, Industry, AgricultureInformation ScienceHealth Care
Listeria monocytogenesListeriosisListeriaMeningitis, ListeriaFood MicrobiologyHemolysin ProteinsBacterial ProteinsVirulencePeptide Termination FactorsCheeseHeat-Shock ProteinsBacterial ToxinsColony Count, MicrobialNisinMeat ProductsFood-Processing IndustryMacrophagesSpleenBacteriocinsGene Expression Regulation, BacterialFood PreservationFood ContaminationFoodborne DiseasesDNA, BacterialMice, Inbred C57BLMolecular Sequence DataMice, Inbred BALB CFood PreservativesMicrobial ViabilityImmunity, InnateVirulence FactorsCulture MediaSerotypingFood HandlingMilkPhagosomesGenes, BacterialImmunity, CellularLethal Dose 50Caco-2 CellsPhagocytosisBacterial AdhesionBenzalkonium CompoundsInterferon-gammaAntigens, BacterialEnvironmental MicrobiologyAnti-Bacterial AgentsCD8-Positive T-LymphocytesSigma FactorBacterial VaccinesImmunity, ActiveMacrophage ActivationBetaineLiverHemolysin FactorsAmpicillinBase SequenceHypersensitivity, DelayedPediococcusPhosphoinositide Phospholipase CMembrane ProteinsBacterial Typing TechniquesGene DeletionHot TemperatureRefrigerationMutationBacterial LoadCold TemperatureMice, KnockoutStainless SteelAmino Acid SequenceHemolysisTemperatureSequence Analysis, DNAImmunologic MemoryAlanine RacemaseAcidsMeatSpecies SpecificityRibotypingImmunization, PassiveCell WallCell LinePeritoneal CavityElectrophoresis, Gel, Pulsed-FieldHydrogen-Ion Concentration
ListeriosisAbstractIntracellular pathogenContaminationStrainsBacteriumPathogensPresence of Listeria monocytogenesSerotypesBacteriaBacterial pathogenOutbreaksPrevalenceStaphylococcusGeneMiceInnocuaInhibitionListeriolysinPathogenesisVirulence factorsSpeciesGenomeATCCGrowthReady-to-eat meatViableMolecularIdentificationMurineColi
Listeriosis4
Abstract1
Intracellular pathogen2
Contamination4
  • WASHINGTON, September 25, 2014- Foster Farms, a Farmerville, La., establishment, is recalling approximately 39,747 pounds of frozen pre-cooked chicken products due to possible contamination with Listeria monocytogenes , the U.S. Department of Agriculture's Food Safety and Inspection Service (FSIS) announced today. (usda.gov)
  • The present study aimed to assess bacteriological contamination with Escherichia coli and Listeria monocytogenes in the raw milk samples of the dairy farms in Golestan province, Iran. (magiran.com)
  • E. coli and L. monocytogenes contamination was significantly higher in traditional dairy farms than industrial dairy farms (P ˂ 0.05). (magiran.com)
  • Survival and recovery of Listeria monocytogenes in the ready-to-eat (RTE) meat processing environment were studied, from environmental contamination to intervention. (iastate.edu)
Strains2
Bacterium1
  • It is the investigators intention to investigate whether a specially designed vaccine, based on a genetically modified strain of the bacterium Listeria monocytogenes and called ADXS11-001 is safe to use and is able to boost the immune system of patients presenting with Human Papilloma Virus (HPV) associated oropharyngeal cancer (OPSCC). (clinicaltrials.gov)
Pathogens1
Presence of Listeria monocytogenes2
Serotypes2
  • The majority of Listeria monocytogenes that have been isolated from food product or human cases are of the serotypes ½ a, ½ b and 4b. (lsu.edu)
  • We observed PMSCs in 35% of L. monocytogenes isolates (n = 54) recovered from the British Columbia food supply, including serotypes 1/2a (30%), 1/2c (100%), and 3a (100%), and a 3-codon deletion (amino acid positions 738 to 740) seen in 57% of 4b isolates from fish-processing facilities. (oregonstate.edu)
Bacteria4
  • The PS-binding receptor TIM-4 (encoded by the Timd4 gene) contributes to efficient cell-to-cell spread by L. monocytogenes in macrophages in vitro and growth of these bacteria is impaired in Timd4 −/− mice. (nature.com)
  • Our results indicate that PS-targeted therapeutics may be useful in the fight against infections by L. monocytogenes and other bacteria that use similar strategies of cell-to-cell spread during infection. (nature.com)
  • Irreversible loss of membrane-binding activity of Listeria -derived cytolysins in non-acidic conditions: a distinct difference from allied cytolysins produced by other Gram-positive bacteria. (nature.com)
  • SIRT2 is recruiter to the transcription star sites of genes repressed during inection leading to H3K18 deacetylation and transcriptional repression.finnaly, my results demonstrate that SIRT2 is hijacked by L monocytogenes and promotes an increase in intracellular bacteria. (archives-ouvertes.fr)
Bacterial pathogen1
Outbreaks1
  • Due to the recent outbreaks, recalls and deaths associated with Listeria monocytogenes in ready-to-eat meat products, the United States Department of Agriculture (USDA), Food Safety and Inspection Service (FSIS) on October 2, 2003 issued a directive for the control of Listeria monocytogenes on ready-to-eat products. (lsu.edu)
Prevalence1
  • This study was conducted to determine the prevalence of VA SNPs in inlA among L. monocytogenes from environments associated with RTE food production and handling. (ttu.edu)
Staphylococcus1
  • This alternative sigma factor contributes to the ability of organisms such as Listeria monocytogenes , Bacillus subtilis , and Staphylococcus aureus to survive under environmental and energy stress conditions ( 4 , 10 , 18 , 19 , 65 ).σ B also contributes to biofilm formation in S. aureus and Staphylococcus epidermidis ( 37 , 55 ). (asm.org)
Gene5
Mice1
Innocua1
  • U jednom od 30 uzoraka je utvrđena prisutnost bakterije Listeria monocytogenes, a u tri uzorka je utvrđena prisutnost Listeria innocua. (nsk.hr)
Inhibition1
Listeriolysin3
  • Alberti-Segui, C., Goeden, K. R. & Higgins, D. E. Differential function of Listeria monocytogenes listeriolysin O and phospholipases C in vacuolar dissolution following cell-to-cell spread. (nature.com)
  • Several virulence factors of Listeria monocytogenes have been identified and extensively characterized at the molecular and cell biologic levels, including the hemolysin (listeriolysin O), two distinct phospholipases, a protein (ActA), several internalins, and others. (nih.gov)
  • Listeriolysin O (LLO) is a major virulence factor secreted by the pathogenic Listeria monocytogenes and acts as pore-forming cytolysin. (microbiologyresearch.org)
Pathogenesis2
Virulence factors1
Species1
  • Immunoassay method in samples of fresh cow cheese, and from the obtained positive samples with Horizontal method for the detection and enumeration of cells and biochemical prove the presence of species Listeria monocytogenes. (nsk.hr)
Genome2
ATCC1
Growth3
Ready-to-eat meat2
  • The survival of Listeria monocytogenes at freezer temperatures on a variety of ready-to-eat meat products was also assessed. (lsu.edu)
  • Our study has been successful in understanding the survival of Listeria monocytogenes at freezer temperatures on the surface of ready-to-eat meat products under vacuum and non-vacuum package storage conditions. (lsu.edu)
Viable1
  • Desiccated cells of L. monocytogenes were not observed to go into viable but non-culturable (VBNC) state well indicating that current methods of using nonselective media, tryptic soy agar with 0.6% yeast extract, is adequate in recovering the majority of the viable cells. (iastate.edu)
Molecular1
  • The human, food and environmental isolates were of the same serotype and were indistinguishable by molecular typing.This is the first community outbreak of L monocytogenes reported in Scotland since the current outbreak surveillance was established in 1996. (elsevier.com)
Identification1
Murine1
Coli4
A Small RNA Controls Expression of the Chitinase ChiA in Listeria monocytogenes
A Small RNA Controls Expression of the Chitinase ChiA in Listeria monocytogenes (journals.plos.org)
Listeria monocytogenes epitopes - Immune Epitope Database (IEDB)
Listeria monocytogenes epitopes - Immune Epitope Database (IEDB) (iedb.org)
Listeria monocytogenes | bacterium | Britannica.com
Listeria monocytogenes | bacterium | Britannica.com (britannica.com)
Listeria Monocytogenes - Methods and Protocols | Edward M. Fox | Springer
Listeria Monocytogenes - Methods and Protocols | Edward M. Fox | Springer (springer.com)
Listeriosis | 1997 Case Definition
Listeriosis | 1997 Case Definition (wwwn.cdc.gov)
Listeria Monocytogenes | Cornell University College of Veterinary Medicine
Listeria Monocytogenes | Cornell University College of Veterinary Medicine (vet.cornell.edu)
Diagnosis and Management of Foodborne Illnesses
Diagnosis and Management of Foodborne Illnesses (cdc.gov)
Encephalitis And Intrauterine Or Cervical Infections In Pregnant Women Em X36300 Stock Photo | Getty Images
Encephalitis And Intrauterine Or Cervical Infections In Pregnant Women Em X36300 Stock Photo | Getty Images (gettyimages.com)
Which of the following statements concerning corrosion | bartleby
Which of the following statements concerning corrosion | bartleby (bartleby.com)
Listeria Monocytogenes Infection (Listeriosis): Background, Pathophysiology, Epidemiology
Listeria Monocytogenes Infection (Listeriosis): Background, Pathophysiology, Epidemiology (emedicine.medscape.com)
5 Common Foodborne Illnesses That You've Never Heard Of - Food Insight
5 Common Foodborne Illnesses That You've Never Heard Of - Food Insight (foodinsight.org)
Heterologous Vaccination and Checkpoint Blockade Synergize To Induce Antileukemia Immunity | The Journal of Immunology
Heterologous Vaccination and Checkpoint Blockade Synergize To Induce Antileukemia Immunity | The Journal of Immunology (jimmunol.org)
Effector γδ T Cell Differentiation Relies on Master but Not Auxiliary Th Cell Transcription Factors | The Journal of Immunology
Effector γδ T Cell Differentiation Relies on Master but Not Auxiliary Th Cell Transcription Factors | The Journal of Immunology (jimmunol.org)
Federal Register :: Draft Interagency Risk Assessment-Listeria monocytogenes
Federal Register :: Draft Interagency Risk Assessment-Listeria monocytogenes (federalregister.gov)
Listeria monocytogenes exploits efferocytosis to promote cell-to-cell spread | Nature
Listeria monocytogenes exploits efferocytosis to promote cell-to-cell spread | Nature (nature.com)
Listeria monocytogenes | MSDSonline
Listeria monocytogenes | MSDSonline (msdsonline.com)
The ISG15 protein plays a key role in fighting bacterial infections | Institut Pasteur
The ISG15 protein plays a key role in fighting bacterial infections | Institut Pasteur (pasteur.fr)
On The Hunt For a Coronavirus Vaccine - BioTechniques
On The Hunt For a Coronavirus Vaccine - BioTechniques (biotechniques.com)
ASM Microbe 2019 Archives - BioTechniques
ASM Microbe 2019 Archives - BioTechniques (biotechniques.com)
Meningitis (Neisseria meningitidis)
Meningitis (Neisseria meningitidis) (giantmicrobes.com)
Granuloma - Wikipedia
Granuloma - Wikipedia (en.m.wikipedia.org)
Salad Recalls | Page 2
Salad Recalls | Page 2 (consumeraffairs.com)
Amrita Health Foods recalls protein bars
Amrita Health Foods recalls protein bars (consumeraffairs.com)
Public Health Alert issued for products associated Almark Foods recall
Public Health Alert issued for products associated Almark Foods recall (consumeraffairs.com)
Nodine's Smokehouse recalls smoked salmon
Nodine's Smokehouse recalls smoked salmon (consumeraffairs.com)
Vimentin Expressed on Mycobacterium tuberculosis-Infected Human Monocytes Is Involved in Binding to the NKp46 Receptor | The...
Vimentin Expressed on Mycobacterium tuberculosis-Infected Human Monocytes Is Involved in Binding to the NKp46 Receptor | The... (jimmunol.org)
May 2006 - Volume 14 - Issue 3 : Infectious Diseases in Clinical Practice
May 2006 - Volume 14 - Issue 3 : Infectious Diseases in Clinical Practice (journals.lww.com)
Difference between revisions of Phage Mediated Biocontrol of Food Borne Bacteria - microbewiki
Difference between revisions of "Phage Mediated Biocontrol of Food Borne Bacteria" - microbewiki (microbewiki.kenyon.edu)
Difference between revisions of Biofilms on food preparation surfaces - microbewiki
Difference between revisions of "Biofilms on food preparation surfaces" - microbewiki (microbewiki.kenyon.edu)