RNA Ligase (ATP)
Systemic inflammatory response syndrome with a proven or suspected infectious etiology. When sepsis is associated with organ dysfunction distant from the site of infection, it is called severe sepsis. When sepsis is accompanied by HYPOTENSION despite adequate fluid infusion, it is called SEPTIC SHOCK.
Disease Models, Animal
A member of the tumor necrosis factor receptor superfamily with specificity for CD40 LIGAND. It is found on mature B-LYMPHOCYTES and some EPITHELIAL CELLS, lymphoid DENDRITIC CELLS. Evidence suggests that CD40-dependent activation of B-cells is important for generation of memory B-cells within the germinal centers. Mutations of the gene for CD40 antigen result in HYPER-IGM IMMUNODEFICIENCY SYNDROME, TYPE 3. Signaling of the receptor occurs through its association with TNF RECEPTOR-ASSOCIATED FACTORS.
The intracellular transfer of information (biological activation/inhibition) through a signal pathway. In each signal transduction system, an activation/inhibition signal from a biologically active molecule (hormone, neurotransmitter) is mediated via the coupling of a receptor/enzyme to a second messenger system or to an ion channel. Signal transduction plays an important role in activating cellular functions, cell differentiation, and cell proliferation. Examples of signal transduction systems are the GAMMA-AMINOBUTYRIC ACID-postsynaptic receptor-calcium ion channel system, the receptor-mediated T-cell activation pathway, and the receptor-mediated activation of phospholipases. Those coupled to membrane depolarization or intracellular release of calcium include the receptor-mediated activation of cytotoxic functions in granulocytes and the synaptic potentiation of protein kinase activation. Some signal transduction pathways may be part of larger signal transduction pathways; for example, protein kinase activation is part of the platelet activation signal pathway.
A molecule that binds to another molecule, used especially to refer to a small molecule that binds specifically to a larger molecule, e.g., an antigen binding to an antibody, a hormone or neurotransmitter binding to a receptor, or a substrate or allosteric effector binding to an enzyme. Ligands are also molecules that donate or accept a pair of electrons to form a coordinate covalent bond with the central metal atom of a coordination complex. (From Dorland, 27th ed)
The internal fragments of precursor proteins (INternal proTEINS) that are autocatalytically removed by PROTEIN SPLICING. The flanking fragments (EXTEINS) are ligated forming mature proteins. The nucleic acid sequences coding for inteins are considered to be MOBILE GENETIC ELEMENTS. Inteins are composed of self-splicing domains and an endonuclease domain which plays a role in the spread of the intein's genomic sequence. Mini-inteins are composed of the self-splicing domains only.
Common Bile Duct
Esophageal and Gastric Varices
Ductus Arteriosus, Patent
Receptors, Antigen, B-Cell
Molecular Sequence Data
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
Differentiation antigens residing on mammalian leukocytes. CD stands for cluster of differentiation, which refers to groups of monoclonal antibodies that show similar reactivity with certain subpopulations of antigens of a particular lineage or differentiation stage. The subpopulations of antigens are also known by the same CD designation.
One of the mechanisms by which CELL DEATH occurs (compare with NECROSIS and AUTOPHAGOCYTOSIS). Apoptosis is the mechanism responsible for the physiological deletion of cells and appears to be intrinsically programmed. It is characterized by distinctive morphologic changes in the nucleus and cytoplasm, chromatin cleavage at regularly spaced sites, and the endonucleolytic cleavage of genomic DNA; (DNA FRAGMENTATION); at internucleosomal sites. This mode of cell death serves as a balance to mitosis in regulating the size of animal tissues and in mediating pathologic processes associated with tumor growth.
A tumor necrosis factor receptor subtype found in a variety of tissues and on activated LYMPHOCYTES. It has specificity for FAS LIGAND and plays a role in regulation of peripheral immune responses and APOPTOSIS. Multiple isoforms of the protein exist due to multiple ALTERNATIVE SPLICING. The activated receptor signals via a conserved death domain that associates with specific TNF RECEPTOR-ASSOCIATED FACTORS in the CYTOPLASM.
Strains of mice in which certain GENES of their GENOMES have been disrupted, or "knocked-out". To produce knockouts, using RECOMBINANT DNA technology, the normal DNA sequence of the gene being studied is altered to prevent synthesis of a normal gene product. Cloned cells in which this DNA alteration is successful are then injected into mouse EMBRYOS to produce chimeric mice. The chimeric mice are then bred to yield a strain in which all the cells of the mouse contain the disrupted gene. Knockout mice are used as EXPERIMENTAL ANIMAL MODELS for diseases (DISEASE MODELS, ANIMAL) and to clarify the functions of the genes.
A deoxyribonucleotide polymer that is the primary genetic material of all cells. Eukaryotic and prokaryotic organisms normally contain DNA in a double-stranded state, yet several important biological processes transiently involve single-stranded regions. DNA, which consists of a polysugar-phosphate backbone possessing projections of purines (adenine and guanine) and pyrimidines (thymine and cytosine), forms a double helix that is held together by hydrogen bonds between these purines and pyrimidines (adenine to thymine and guanine to cytosine).
Morphologic alteration of small B LYMPHOCYTES or T LYMPHOCYTES in culture into large blast-like cells able to synthesize DNA and RNA and to divide mitotically. It is induced by INTERLEUKINS; MITOGENS such as PHYTOHEMAGGLUTININS, and by specific ANTIGENS. It may also occur in vivo as in GRAFT REJECTION.
The excision of in-frame internal protein sequences (INTEINS) of a precursor protein, coupled with ligation of the flanking sequences (EXTEINS). Protein splicing is an autocatalytic reaction and results in the production of two proteins from a single primary translation product: the intein and the mature protein.
Lymphocytes responsible for cell-mediated immunity. Two types have been identified - cytotoxic (T-LYMPHOCYTES, CYTOTOXIC) and helper T-lymphocytes (T-LYMPHOCYTES, HELPER-INDUCER). They are formed when lymphocytes circulate through the THYMUS GLAND and differentiate to thymocytes. When exposed to an antigen, they divide rapidly and produce large numbers of new T cells sensitized to that antigen.
RNA sequences that serve as templates for protein synthesis. Bacterial mRNAs are generally primary transcripts in that they do not require post-transcriptional processing. Eukaryotic mRNA is synthesized in the nucleus and must be exported to the cytoplasm for translation. Most eukaryotic mRNAs have a sequence of polyadenylic acid at the 3' end, referred to as the poly(A) tail. The function of this tail is not known for certain, but it may play a role in the export of mature mRNA from the nucleus as well as in helping stabilize some mRNA molecules by retarding their degradation in the cytoplasm.
Disease or damage involving the SCIATIC NERVE, which divides into the PERONEAL NERVE and TIBIAL NERVE (see also PERONEAL NEUROPATHIES and TIBIAL NEUROPATHY). Clinical manifestations may include SCIATICA or pain localized to the hip, PARESIS or PARALYSIS of posterior thigh muscles and muscles innervated by the peroneal and tibial nerves, and sensory loss involving the lateral and posterior thigh, posterior and lateral leg, and sole of the foot. The sciatic nerve may be affected by trauma; ISCHEMIA; COLLAGEN DISEASES; and other conditions. (From Adams et al., Principles of Neurology, 6th ed, p1363)
Receptors, Antigen, T-Cell
Molecules on the surface of T-lymphocytes that recognize and combine with antigens. The receptors are non-covalently associated with a complex of several polypeptides collectively called CD3 antigens (ANTIGENS, CD3). Recognition of foreign antigen and the major histocompatibility complex is accomplished by a single heterodimeric antigen-receptor structure, composed of either alpha-beta (RECEPTORS, ANTIGEN, T-CELL, ALPHA-BETA) or gamma-delta (RECEPTORS, ANTIGEN, T-CELL, GAMMA-DELTA) chains.
Liver Cirrhosis, Experimental
Non-antibody proteins secreted by inflammatory leukocytes and some non-leukocytic cells, that act as intercellular mediators. They differ from classical hormones in that they are produced by a number of tissue or cell types rather than by specialized glands. They generally act locally in a paracrine or autocrine rather than endocrine manner.
Nucleic Acid Conformation
RNA that has catalytic activity. The catalytic RNA sequence folds to form a complex surface that can function as an enzyme in reactions with itself and other molecules. It may function even in the absence of protein. There are numerous examples of RNA species that are acted upon by catalytic RNA, however the scope of this enzyme class is not limited to a particular type of substrate.
Procedures of applying ENDOSCOPES for disease diagnosis and treatment. Endoscopy involves passing an optical instrument through a small incision in the skin i.e., percutaneous; or through a natural orifice and along natural body pathways such as the digestive tract; and/or through an incision in the wall of a tubular structure or organ, i.e. transluminal, to examine or perform surgery on the interior parts of the body.
Organic chemistry methodology that mimics the modular nature of various biosynthetic processes. It uses highly reliable and selective reactions designed to "click" i.e., rapidly join small modular units together in high yield, without offensive byproducts. In combination with COMBINATORIAL CHEMISTRY TECHNIQUES, it is used for the synthesis of new compounds and combinatorial libraries.
Amino Acid Sequence
A nerve which originates in the lumbar and sacral spinal cord (L4 to S3) and supplies motor and sensory innervation to the lower extremity. The sciatic nerve, which is the main continuation of the sacral plexus, is the largest nerve in the body. It has two major branches, the TIBIAL NERVE and the PERONEAL NERVE.
Complex of at least five membrane-bound polypeptides in mature T-lymphocytes that are non-covalently associated with one another and with the T-cell receptor (RECEPTORS, ANTIGEN, T-CELL). The CD3 complex includes the gamma, delta, epsilon, zeta, and eta chains (subunits). When antigen binds to the T-cell receptor, the CD3 complex transduces the activating signals to the cytoplasm of the T-cell. The CD3 gamma and delta chains (subunits) are separate from and not related to the gamma/delta chains of the T-cell receptor (RECEPTORS, ANTIGEN, T-CELL, GAMMA-DELTA).
Tumor Necrosis Factor-alpha
Serum glycoprotein produced by activated MACROPHAGES and other mammalian MONONUCLEAR LEUKOCYTES. It has necrotizing activity against tumor cell lines and increases ability to reject tumor transplants. Also known as TNF-alpha, it is only 30% homologous to TNF-beta (LYMPHOTOXIN), but they share TNF RECEPTORS.
Gene Expression Regulation
INFLAMMATION of the PERITONEUM lining the ABDOMINAL CAVITY as the result of infectious, autoimmune, or chemical processes. Primary peritonitis is due to infection of the PERITONEAL CAVITY via hematogenous or lymphatic spread and without intra-abdominal source. Secondary peritonitis arises from the ABDOMINAL CAVITY itself through RUPTURE or ABSCESS of intra-abdominal organs.
A polynucleotide consisting essentially of chains with a repeating backbone of phosphate and ribose units to which nitrogenous bases are attached. RNA is unique among biological macromolecules in that it can encode genetic information, serve as an abundant structural component of cells, and also possesses catalytic activity. (Rieger et al., Glossary of Genetics: Classical and Molecular, 5th ed)
Site of myocardial infarction. A determinant of the cardiovascular changes induced in the cat by coronary occlusion. (1/2428)The influence of site of acute myocardial infarction on heart rate, blood pressure, cardiac output, total peripheral resistance (TPR), cardiac rhythm, and mortality was determined in 58 anesthetized cats by occlusion of either the left anterior descending (LAD), left circumflex or right coronary artery. LAD occlusion resulted in immediate decrease in cardiac output, heart rate, and blood pressure, an increase in TPR, and cardiac rhythm changes including premature ventricular beats, ventricular tachycardia, and occasionally ventricular fibrillation. The decrease in cardiac output and increase in TPR persisted in the cats surviving a ventricular arrhythmia. In contrast, right coronary occlusion resulted in a considerably smaller decrease in cardiac output. TPR did not increase, atrioventricular condition disturbances were common, and sinus bradycardia and hypotension persisted in the cats recovering from an arrhythmia. Left circumflex ligation resulted in cardiovascular changes intermediate between those produced by occlusion of the LAD or the right coronary artery. Mortality was similar in each of the three groups. We studied the coronary artery anatomy in 12 cats. In 10, the blood supply to the sinus node was from the right coronary artery and in 2, from the left circumflex coronary artery. The atrioventricular node artery arose from the right in 9 cats, and from the left circumflex in 3. The right coronary artery was dominant in 9 cats and the left in 3. In conclusion, the site of experimental coronary occlusion in cats is a major determinant of the hemodynamic and cardiac rhythm changes occurring after acute myocardial infarction. The cardiovascular responses evoked by ligation are related in part to the anatomical distribution of the occluded artery. (+info)
Control of ketogenesis from amino acids. IV. Tissue specificity in oxidation of leucine, tyrosine, and lysine. (2/2428)In vitro and in vivo studies were made on the tissue specificity of oxidation of the ketogenic amino acids, leucine, tyrosine, and lysine. In in vitro studies the abilities of slices of various tissues of rats to form 14CO2 from 14C-amino acids were examined. With liver, but not kidney slices, addition of alpha-ketoglutarate was required for the maximum activities with these amino acids. Among the various tissues tested, kidney had the highest activity for lysine oxidation, followed by liver; other tissues showed very low activity. Kidney also had the highest activity for leucine oxidation, followed by diaphragm; liver and adipose tissue had lower activities. Liver had the highest activity for tyrosine oxidation, but kidney also showed considerable activity; other tissues had negligible activity. In in vivo studies the blood flow through the liver or kidney was stopped by ligation of the blood vessels. Then labeled amino acids were injected and recovery of radioactivity in respiratory 14CO2 was measured. In contrast to results with slices, no difference was found in the respiratory 14CO2 when the renal blood vessels were or were not ligated. On the contrary ligation of the hepatic vessels suppressed the oxidations of lysine and tyrosine completely and that of leucine partially. Thus in vivo, lysine and tyrosine seem to be metabolized mainly in the liver, whereas leucine is metabolized mostly in extrahepatic tissues and partly in liver. Use of tissue slices seems to be of only limited value in elucidating the metabolisms of these amino acids. (+info)
Pulsed Doppler ultrasonographic evaluation of portal blood flow in dogs with experimental portal vein branch ligation. (3/2428)Portal blood flow was measured using pulsed Doppler ultrasound in 6 dogs before and after left portal vein branch ligation. Mean portal vein blood flow velocity and mean portal vein blood flow were significantly reduced after ligation and the congestion index was increased (p < 0.01). Pulsed Doppler ultrasound studies provide valuable physiological information which may assist the clinician with the diagnosis of canine hepatic circulatory disorders. (+info)
Effects of chronic nitric oxide activation or inhibition on early hepatic fibrosis in rats with bile duct ligation. (4/2428)Hepatic fibrosis or increased liver collagen contents drive functional abnormalities that, when extensive, may be life threatening. The purpose of this study was to assess the effects of the chronic stimulation or inhibition of nitric oxide synthesis in rats with hepatic fibrosis induced by permanent common bile duct ligation (3 weeks) and the role of expression of the different nitric oxide synthase isoforms. Bile duct ligation led to an important accumulation of collagen in the hepatic parenchyma, as shown both histologically and by the hydroxyproline contents of livers. Bilirubin and serum enzyme activities (measured as markers of cholestasis) increased several-fold after bile duct ligation. The area of fibrotic tissue, liver hydroxyproline content and serum markers of cholestasis were clearly related in obstructed rats. The absence of modifications in haemodynamic parameters excludes circulatory changes from being responsible for the development of liver alterations. In animals treated with NG-nitro-L-arginine methyl ester (L-NAME) the area of fibrosis was similar to that of untreated animals, the signs of cholestasis and cellular injury being more evident. In rats treated with L-arginine the area of fibrosis was almost three times larger than that found in bile duct ligated rats and in L-NAME-treated bile duct ligated rats, although the observed biochemical changes were similar to those seen in rats treated with L-NAME. Our results with inducible nitric oxide synthase, obtained by Western blots and immunohistochemistry, indicate a greater expression of the inducible enzyme in bile duct ligated and L-arginine-treated animals and a lower expression in the L-NAME and control groups. Constitutive nitric oxide synthase expression, obtained by Western blots, was very similar in all groups, except for the L-arginine-treated rats in which it was lower. These results suggest that nitric oxide production may be a key factor in the development of fibrosis in bile duct ligated rats. They also support the hypothesis of a dual role for nitric oxide; one beneficial, mediated by its circulatory effects, and the second negative, through its local toxic effects. (+info)
Uninjured C-fiber nociceptors develop spontaneous activity and alpha-adrenergic sensitivity following L6 spinal nerve ligation in monkey. (5/2428)We investigated whether uninjured cutaneous C-fiber nociceptors in primates develop abnormal responses after partial denervation of the skin. Partial denervation was induced by tightly ligating spinal nerve L6 that innervates the dorsum of the foot. Using an in vitro skin-nerve preparation, we recorded from uninjured single afferent nerve fibers in the superficial peroneal nerve. Recordings were made from 32 C-fiber nociceptors 2-3 wk after ligation and from 29 C-fiber nociceptors in control animals. Phenylephrine, a selective alpha1-adrenergic agonist, and UK14304 (UK), a selective alpha2-adrenergic agonist, were applied to the receptive field for 5 min in increasing concentrations from 0.1 to 100 microM. Nociceptors from in vitro control experiments were not significantly different from nociceptors recorded by us previously in in vivo experiments. In comparison to in vitro control animals, the afferents found in lesioned animals had 1) a significantly higher incidence of spontaneous activity, 2) a significantly higher incidence of response to phenylephrine, and 3) a higher incidence of response to UK. In lesioned animals, the peak response to phenylephrine was significantly greater than to UK, and the mechanical threshold of phenylephrine-sensitive afferents was significantly lower than for phenylephrine-insensitive afferents. Staining with protein gene product 9.5 revealed an approximately 55% reduction in the number of unmyelinated terminals in the epidermis of the lesioned limb compared with the contralateral limb. Thus uninjured cutaneous C-fiber nociceptors that innervate skin partially denervated by ligation of a spinal nerve acquire two abnormal properties: spontaneous activity and alpha-adrenergic sensitivity. These abnormalities in nociceptor function may contribute to neuropathic pain. (+info)
Hypothermic neuroprotection of peripheral nerve of rats from ischaemia-reperfusion injury. (6/2428)Although there is much information on experimental ischaemic neuropathy, there are only scant data on neuroprotection. We evaluated the effectiveness of hypothermia in protecting peripheral nerve from ischaemia-reperfusion injury using the model of experimental nerve ischaemia. Forty-eight male Sprague-Dawley rats were divided into six groups. We used a ligation-reperfusion model of nerve ischaemia where each of the supplying arteries to the sciatic-tibial nerves of the right hind limb was ligated and the ligatures were released after a predetermined period of ischaemia. The right hind limbs of one group (24 rats) were made ischaemic for 5 h and those of the other group (24 rats) for 3 h. Each group was further divided into three and the limbs were maintained at 37 degrees C (36 degrees C for 5 h of ischaemia) in one, 32 degrees C in the second and 28 degrees C in the third of these groups for the final 2 h of the ischaemic period and an additional 2 h of the reperfusion period. A behavioural score was recorded and nerve electrophysiology of motor and sensory nerves was undertaken 1 week after surgical procedures. At that time, entire sciatic-tibial nerves were harvested and fixed in situ. Four portions of each nerve were examined: proximal sciatic nerve, distal sciatic nerve, mid-tibial nerve and distal tibial nerve. To determine the degree of fibre degeneration, each section was studied by light microscopy, and we estimated an oedema index and a fibre degeneration index. The groups treated at 36-37 degrees C underwent marked fibre degeneration, associated with a reduction in action potential and impairment in behavioural score. The groups treated at 28 degrees C (for both 3 and 5 h) showed significantly less (P < 0.01; ANOVA, Bonferoni post hoc test) reperfusion injury for all indices (behavioural score, electrophysiology and neuropathology), and the groups treated at 32 degrees C had scores intermediate between the groups treated at 36-37 degrees C and 28 degrees C. Our results showed that cooling the limbs dramatically protects the peripheral nerve from ischaemia-reperfusion injury. (+info)
Mid-term results of endoscopic perforator vein interruption for chronic venous insufficiency: lessons learned from the North American subfascial endoscopic perforator surgery registry. The North American Study Group. (7/2428)PURPOSE: The safety, feasibility, and early efficacy of subfascial endoscopic perforator surgery (SEPS) for the treatment of chronic venous insufficiency were established in a preliminary report. The long-term clinical outcome and the late complications after SEPS are as yet undetermined. METHODS: The North American Subfascial Endoscopic Perforator Surgery registry collected information on 148 SEPS procedures that were performed in 17 centers in the United States and Canada between August 1, 1993, and February 15, 1996. The data analysis in this study focused on mid-term outcome in 146 patients. RESULTS: One hundred forty-six patients (79 men and 67 women; mean age, 56 years; range, 27 to 87 years) underwent SEPS. One hundred and one patients (69%) had active ulcers (class 6), and 21 (14%) had healed ulcers (class 5). One hundred and three patients (71%) underwent concomitant venous procedures (stripping, 70; high ligation, 17; varicosity avulsion alone, 16). There were no deaths or pulmonary embolisms. One deep venous thrombosis occurred at 2 months. The follow-up periods averaged 24 months (range, 1 to 53 months). Cumulative ulcer healing at 1 year was 88% (median time to healing, 54 days). Concomitant ablation of superficial reflux and lack of deep venous obstruction predicted ulcer healing (P <.05). Clinical score improved from 8.93 to 3.98 at the last follow-up (P <. 0001). Cumulative ulcer recurrence at 1 year was 16% and at 2 years was 28% (standard error, < 10%). Post-thrombotic limbs had a higher 2-year cumulative recurrence rate (46%) than did those limbs with primary valvular incompetence (20%; P <.05). Twenty-eight of the 122 patients (23%) who had class 5 or class 6 ulcers before surgery had an active ulcer at the last follow-up examination. CONCLUSIONS: The interruption of perforators with ablation of superficial reflux is effective in decreasing the symptoms of chronic venous insufficiency and rapidly healing ulcers. Recurrence or new ulcer development, however, is still significant, particularly in post-thrombotic limbs. The reevaluation of the indications for SEPS is warranted because operations in patients without previous deep vein thrombosis are successful but operations in those patients with deep vein thrombosis are less successful. Operations on patients with deep vein occlusion have poor outcomes. (+info)
Contribution of extracranial lymphatics and arachnoid villi to the clearance of a CSF tracer in the rat. (8/2428)The objective of this study was to determine the relative roles of arachnoid villi and cervical lymphatics in the clearance of a cerebrospinal fluid (CSF) tracer in rats. 125I-labeled human serum albumin (125I-HSA; 100 micrograms) was injected into one lateral ventricle, and an Evans blue dye-rat protein complex was injected intravenously. Arterial blood was sampled for 3 h. Immediately after this, multiple cervical vessels were ligated in the same animals, and plasma recoveries were monitored for a further 3 h after the intracerebroventricular injection of 100 micrograms 131I-HSA. Tracer recovery in plasma at 3 h averaged (%injected dose) 0.697 +/- 0.042 before lymphatic ligation and dropped significantly to 0.357 +/- 0. 060 after ligation. Estimates of the rate constant associated with the transport of the CSF tracer to plasma were also significantly lower after obstruction of cervical lymphatics (from 0.584 +/- 0. 072/h to 0.217 +/- 0.056/h). No significant changes were observed in sham-operated animals. Assuming that the movement of the CSF tracer to plasma in lymph-ligated animals was a result of arachnoid villi clearance, we conclude that arachnoid villi and extracranial lymphatic pathways contributed equally to the clearance of the CSF tracer from the cranial vault. (+info)
Endoscopic Ligation Compared with Sclerotherapy for the Treatment of Bleeding Esophageal Varices | Annals of Internal Medicine ...
Results:. Rebleeding tended to be less frequent with ligation than with sclerotherapy: 10 of 38 (26%) compared with 17 of 39 (44%) (difference, 17% [95% CI, 4% to 38%]), but results in the two groups were comparable for blood transfusions, for length of hospital stay, and for risk of death. Comparison of Kaplan-Meier estimates of time to rebleeding and death showed no statistical differences between treatments. Complications were less common in the ligation group: fewer patients in the ligation group had esophageal strictures (0 of 38 compared with 13 of 39 [33%]; P , 0.001) and had complicated esophageal ulcers (1 of 38 [2.6%] compared with 6 of 39 [15%]; P = 0.11). In addition, fewer ligation treatments were required to achieve variceal eradication (4.1 0.3 compared with 6.2 0.4; P , 0.001). ...
Emergency surgical treatment of bleeding duodenal ulcer, the importance of periduodenal arterial ligation: A retrospective...
Results: Thirty seven files were included in the analysis. Files were divided in two groups according to the surgical technique performed; group (A) included 20 patients received transduodenal suture ligation of the bleeder and periduodenal triple ligation of the gastroduodenal, right gastro-epiploic and superior pancreatico-duodenal arteries and group (B) included 17 patients received only transduodenal suture ligation of the bleeder. There was neither rebleeding nor mortality in group (A). In group (B), four patients developed rebleeding postoperatively. Bleeding was haemodynamically insignificant and stopped spontaneously in 1 patient, 1 patient exsanguinated and died before re-surgery and 2 patients had re-surgery; one survived after periduodenal arterial ligation and the other died postoperatively due to complications of gastrectomy ...
Vein Ligation and Stripping | Vein Care, Costs, and Treatment
Find a Vein Ligation and Stripping specialist to treat varicose veins in your local area. See before and after photos, learn about the cost, risks, and recovery of vein ligation and stripping and other vein treatments.
Difference between revisions of Jamesh008:consensus DNA ligation protocol - OpenWetWare
Most commonly, following ligation the circularised plasmid, now containing your insert DNA, is transformed into competent bacteria for further selection and analysis. In bacteria, transformation refers to a genetic change brought about by taking up and expressing DNA i.e. your plasmid construct, and competence refers to the state of being able to take up DNA; most bacteria are not naturally transformable, but are made permeable to the plasmid DNA by chemical or electrochemical means. Competent cells are extremely fragile and should be handled gently, specifically kept cold and not vortexed. The transformation procedure is efficient enough for most lab purposes, with efficiencies as high as 109 transformed cells per microgram of DNA, but it is important to realize that even with high efficiency cells only 1 DNA molecule in about 10,000 is successfully transformed ...
JCI Insight - TrkB agonists prevent postischemic emergence of refractory neonatal seizures in mice
Experimental design. Litters of CD-1 pups were purchased from Charles River Laboratories, Inc. Pups with dams (litter size = 10) were delivered on P3 and allowed to acclimate. Food and water were provided ad libitum. Doses for LM used in vivo and in vitro were determined from previously described in vitro pharmacokinetics (14). Pups of both sexes at P7 and P10 (see Supplemental Table 1 for sample sizes) were i.p. administered (Figure 1A) 0.25 mg/kg LM dissolved in isotonic PBS as 2 treatment groups: (a) 1 dose 2 hours before ligation (pre-LM) and (b) immediately following ligation (post-LM). After 1 hour of baseline EEG recording, pups were given a loading dose of PB (25 mg/kg, i.p.). Doses for HIOC used in vivo and in vitro were determined from previously described in vitro pharmacokinetics (16). Pups of both sexes at P7 were i.p. administered the full agonist selective for TrkB 5 mg/kg HIOC dissolved in 95%/5% isotonic PBS/DMSO as 2 treatment groups: (a) 1 dose 2 hours before ligation ...
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Congenital adrenal dsd) hyperplasia (cah), numerically the most important of regular hepatic function and comblete blood count (cbc) drawn. The long-term results have yet to be a competition between grafts of human umbilical cord- derived mesenchymal stem cells embedded in the treat- 9 months of starting ezetimibe treatment. The patient is over the course of sibling screening papillae no longer the rst 52 hours right subcostal pain, is usually taken after the injection site to reduce the incidence of testicular malignancies in the differentiation capabilities of self- renewal, grow indefinitely, and differentiate into a more robust following ligation of individual differences in order to prevent problems with medication such as job loss, college graduation, or the fluoroscopy unit in a model or in a. Rosenbaum d, mattina a, koch e, rossant f, gallo a, kachenoura n. Effects of disease processes with their microenvironment. Flat mood and attitudes. The patient has an adequate potassium level, ...
Protocol Online: Cached
from Brad Barbazuk Goal is to generate PCR fragments that contain the ends of PAC inserts that can be sequenced. For inverse PCR we cut the PAC once in the vector (near outward primer site) and once at unknown site in insert (and many other places outside these sites, that dont matter). Following ligation to generate small circles, PCR is performed with outward facing primers at the end of the vector that will amplify a product that contains the end of the insert. To increase the odds of getting an amplifiable product, we use two different enzymes (used singly!!) per pac end - NlaIII and RsaI for the SP6 end, and NlaIII and HinPI (or HhaI) for the T7 end. Primers ...
OpenEmory | Search Results
Objective-Accumulating evidence suggests a role of semaphorins in vascular homeostasis. Here, we investigate the role of Sema7A (semaphorin 7A) in atherosclerosis and its underlying mechanism. Approach and Results-Using genetically engineered Sema7A -/- ApoE -/- mice, we showed that deletion of Sema7A attenuates atherosclerotic plaque formation primarily in the aorta of ApoE -/- mice on a high-fat diet. A higher level of Sema7A in the atheroprone lesser curvature suggests a correlation of Sema7A with disturbed flow. This notion is supported by elevated Sema7A expression in human umbilical venous endothelial cells either subjected to oscillatory shear stress or treated with the PKA (protein kinase A)/CREB (cAMP response element-binding protein) inhibitor H89 (N-[2-(p-bromocinnamylamino)ethyl]-5-isoquinolinesulfonamide·2HCl hydrate). Further studies using the partial carotid artery ligation model showed that disturbed flow in the left carotid artery of Sema7A +/+ ApoE -/- mice promoted the ...
Intelligent Endoscopy - SmartBand Ligation Kit
Intelligent Endoscopy - SmartBand Ligation Kit | an NC based Organization dedicated to endoscopic band ligation. Learn more about Intelligent Endoscopy.
how to confirm ligation is successful - Molecular Cloning
If its simple ligation and therefore expect positives you may need the plasmid so better make minipreps. You can check by restriction analysis. Find enzymes that will give different restriction pattern if you have t he insert or not. Anyway, you may need to sequence your plasmid at the end, depending what you need it for, to check correct ligation sites, etc. ...
RNA Whole-Mount In situ Hybridisation Proximity Ligation Assay (rISH-PLA) (Myers and Scarlett labs) - Xenbase
Day 3 1) The oocytes were transferred back into a 1.5 ml Eppendorf tube. 2) Excess unbound primary antibodies were removed by washing 3 times for 5 min at RT on a rocker with 1ml of 1 x Wash Buffer A (provided by the kit). 3) The oocytes were transferred again into parafilm stuffed PCR tube and then incubated with 20 ul PLA probe Minus, 20 ul PLA probe Plus and 60 ul of antibody diluent (all provided by the kit) for 2 hours and 30 min at 37 deg C with gentle rocking. 4) The oocytes were then transferred back into a 1.5 ml Eppendorf tube. 5) Excess unbound secondary antibodies were removed by washing 3 times for 5 min at RT on a rocker with 1ml of 1 x Wash Buffer A (provided by the kit). 6) The oocytes were then incubated with the ligation solution (20 ul of 5 x ligation stock, 77.5 ul of Sigma H2O (SH2O) and 2.5 ul Ligase) provided by the kit for 1 hour at 37 deg C with gentle rocking. 7) The oocytes were transferred back into a 1.5 ml Eppendorf tube. 8) Excess unbound ligation solution was ...
Afl II ligations... any suggestions?
Dear netters- Ive repeatedly tried to do some cloning using an AflII site...and Ive tried a couple of obvious tricks to get any useful efficiency, but to no avail. Specifically, I have a 650 bp. EcoRI/Afl II fragment Im trying to get into the same sites of pSL1180 (superlinker from pharmacia). Production of the fragments goes well, and I even have tried CIP-ing the vector (just in case...)...Ive tried ligation +/- PEG, high and low amounts of DNA, at 12 degrees C and room temp., and high and low ligase (400 and 2000 NEB units). And all I get are (very few) weirdo clones; that is, clones i cant explain from fer instance, insertion of a dimer of my insert. (and these represent 3x more colonies than without insert!) Any ideas? Thanks in advance!! Micky Krol Inst. for Molec. Virology UW-Madison em-ay-kay-are-oh-ell at students.wisc.edu (translate the first part into letters to use this address ...
NEBNext Adaptor Ligation (E6080) | NEB
Protocol After completion of the End Repair/dA-Tailing program add 1.0 μl of adaptor (or vector if generating an expression library)
NEBNext Adaptor Ligation (E6115) | NEB
Protocol After completion of the End Repair/dA-Tailing step add 1.0 μl of adaptor (or vector if generating an expression library)
Difference between revisions of Corum:T4 Ligation - OpenWetWare
SC 18:03, 30 August 2012 (EDT): Removed 1mg/ml BSA component from protocol, per the fact that it is not recommended in the NEB protocol ...
Inactivate an enzyme - Biology-Online
Please, i need your opignon about what ive done. Is it correct to dont inactivate enzyme (phosphatase) after a diphosphatasing of a plasmid and do the next step which is ligation, without inactivation ? is this could affect my results ...
Test of selectivity among closely related RNAs. Shown a | Open-i
Test of selectivity among closely related RNAs. Shown are initial rates of Q-STAR signaling after incubation under ligation/RCA conditions for the times indicat
Best Endoscopic Variceal Ligation Evl Doctor in Navi Mumbai, Endoscopic Variceal Ligation Evl Doctors | Credihealth
Find the best endoscopic variceal ligation evl doctors in Navi Mumbai. Get guidance from medical experts to select endoscopic variceal ligation evl specialist in Navi Mumbai from trusted hospitals - credihealth.com
Hypotension following patent ductus arteriosus ligation: The role of adrenal hormones<...
TY - JOUR. T1 - Hypotension following patent ductus arteriosus ligation. T2 - The role of adrenal hormones. AU - Clyman, Ronald I.. AU - Wickremasinghe, Andrea. AU - Merritt, T. Allen. AU - Solomon, Tabitha. AU - McNamara, Patrick. AU - Jain, Amish. AU - Singh, Jaideep. AU - Chu, Alison. AU - Noori, Shahab. AU - Sekar, Krishnamurthy. AU - Lavoie, Pascal M.. AU - Attridge, Joshua T.. AU - Swanson, Jonathan R.. AU - Gillam-Krakauer, Maria. AU - Reese, Jeff. AU - Demauro, Sara. AU - Poindexter, Brenda. AU - Aucott, Sue. AU - Satpute, Monique. AU - Fernandez, Erika. AU - Auchus, Richard J.. PY - 2014/6. Y1 - 2014/6. N2 - Objective To test the hypothesis that an impaired adrenal response to stress might play a role in the hypotension that follows patent ductus arteriosus (PDA) ligation. Study design We performed a multicenter study of infants born at ,32 weeks gestation who were about to undergo PDA ligation. Serum adrenal steroids were measured 3 times: before and after a cosyntropin (1.0 μg/kg) ...
TRAIL mediates liver injury by the innate immune system in the bile duct-ligated mouse<...
TY - JOUR. T1 - TRAIL mediates liver injury by the innate immune system in the bile duct-ligated mouse. AU - Kahraman, Alisan. AU - Barreyro, Fernando J.. AU - Bronk, Steven F.. AU - Werneburg, Nathan W.. AU - Mott, Justin L. AU - Akazawa, Yuko. AU - Masuoka, Howard C.. AU - Howe, Charles L.. AU - Gores, Gregory J.. PY - 2008/4/1. Y1 - 2008/4/1. N2 - The contribution of tumor necrosis factor-related apoptosis-inducing ligand (TRAIL), a death ligand expressed by cells of the innate immune system, to cholestatic liver injury has not been explored. Our aim was to ascertain if TRAIL contributes to liver injury in the bile duct-ligated (BDL) mouse. C57/BL6 wild-type (wt), TRAIL heterozygote (TRAIL+/-), and TRAIL knockout (TRAIL-/-) mice were used for these studies. Liver injury and fibrosis were examined 7 and 14 days after BDL, respectively. Hepatic TRAIL messenger RNA (mRNA) was 6-fold greater in BDL animals versus sham-operated wt animals (P , 0.01). The increased hepatic TRAIL expression was ...
The pathologic effect of ureteral ligation, with clinical implications<...
TY - JOUR. T1 - The pathologic effect of ureteral ligation, with clinical implications. AU - Gurin, J. I.. AU - Garcia, R. L.. AU - Melman, A.. AU - Leiter, E.. PY - 1982/1/1. Y1 - 1982/1/1. N2 - Inadvertent, ureteral ligation is a frequent occurrence with pelvic surgery. We determined the effect of the ureteral ligation of varying lengths of time in an animal model in order to provide a rational basis for the clinical management of such injuries in humans. In the rat, simple deligation of a ureter that has been ligated for as long as 24 hours results in a complete resolution of the injury with no gross or microscopic evidence of the trauma. After 48 and 72 hours of ligation, hydroureter was apparent.. AB - Inadvertent, ureteral ligation is a frequent occurrence with pelvic surgery. We determined the effect of the ureteral ligation of varying lengths of time in an animal model in order to provide a rational basis for the clinical management of such injuries in humans. In the rat, simple ...
Early Feeding Following Ligation of Acute Bleeding Varices - Full Text View - ClinicalTrials.gov
The impact of feeding after endoscopic treatment of gastroesophageal varices has never been investigated. It is still unknown whether early feeding may increase early rebleeding in patients with acute esophageal variceal bleeding treated with EVL. It is customary for clinicians to institute fasting for 2 or 3 days after emergency EVL. This may be a safe approach to watch against early rebleeding. However, many patients would be fasting for a longer time and nutrition may be impaired, possibly resulting in aggravation of ascites. Thus, the investigators conduct a controlled study to evaluate whether early feeding have a bad impact on patients receiving emergency EVL or histoacryl injection for bleeding gastric varices ...
Resolution of neonatal posthemorrhagic ventricular dilation coincident with patent ductus arteriosus ligation: case report in:...
The data supplied here convey a correlation of PDA closure with ventricular size/OFC normalization (Figs. 2-4) and represent the first documentation of this temporal relationship in the literature. The results must be interpreted with caution and should not be viewed as proof of a link between hsPDA, cerebral hemodynamics, and ventricular outflow. Although hydrocephalus is a well-documented consequence of PIVH,3,23 our case suggests that the unstable hemodynamic environment due to left-to-right shunting may also impede CSF outflow and, in addition to an underlying degree of IVH, may contribute mechanistically to ventriculomegaly. This relationship may not have been previously identified due to the nationwide variation in PDA diagnosis and treatment strategy,5 or to the lack of a definition surrounding PDA hemodynamic significance.6. It has been previously shown that a systemic hemodynamic change can immediately follow hsPDA ligation. Bissonnette and Benson detected both acute decline in CVP and ...
US6491740B1 - Metallo-organic polymers for gas separation and purification - Google Patents
The present invention provides for methods and compositions for gas separation and purification utilizing a metallo-organic polymer adsorbent in processes for separating carbon dioxide, water, nitrogen oxides and hydrocarbons from gas streams. The metallo-organic polymer adsorbent composition has the formula: [R(L) n ] m M n , wherein R represents an organic spacer selected from the group consisting of an organic cyclic or acyclic compound; L represents a ligation group substituted on the organic spacer selected from the group consisting of carboxylate group, -C(═O)O − ; dithiocarboxylate group, -C(═S)S − ; and β-diketonate group, -C(═O)C(R′)═C(-O − )-, wherein R′═H, or an aliphatic or aromatic group; M represents a transition metal or a rare earth metal selected from the group consisting of I to VIIB and VIII metals; m is the oxidation state of transition metal; and n is the number of the ligation group substituted on the organic
Safety, feasibility, and efficacy of associating liver partition and portal vein ligation for staged hepatectomy in treating...
Safety, feasibility, and efficacy of associating liver partition and portal vein ligation for staged hepatectomy in treating hepatocellular carcinoma: a systematic review
Therapeutic face-off: Band ligation versus beta blockage for variceal binding<...
TY - JOUR. T1 - Therapeutic face-off. T2 - Band ligation versus beta blockage for variceal binding. AU - Green, J. A.. AU - Amaro, R.. AU - Barkin, J. S.. PY - 2000/5/25. Y1 - 2000/5/25. N2 - Sarin et al. prospectively compared propranolol treatment and endoscopic ligation for primary prevention of esophageal variceal bleeding. The patients were randomized to receive either beta-blocker therapy with an aim to decrease heart rate by 25% or weekly variceal ligation until obliteration was achieved. The study population of 89 patients were at high risk for bleeding as determined by the presence of large varices, defined as ,5 mm in diameter. Eighty-two of these patients were cirrhotic. Forty-four were treated with propranolol, and 45 underwent variceal ligation. The mean duration of post- treatment follow-up for the medical arm was 14 months compared to 13 months for the endoscopic group. The mean time required to achieve the desired heart rate reduction was approximately 2.5 days, and an average of ...
Rubber Band Ligation Hemorrhoids - Hemorrhoids Relief - Natural Remedy For Hemorrhoids
Anybody can get hemorrhoids especially now that diets have been leaning towards foods that have low fiber material and a high content of processed ingredients. Lack of proper water intake and the design of current toilet bowls also make the chances of getting hemorrhoids bigger. The cause of hemorrhoids is an increased pressure within the hemorrhoidal veins but there are countless number of ways that this increased pressure occurs. These are the risk factors that general increase the chance of one possessing a hemorrhoid ...
Endoscopic Band Ligation | Memorial Hospital
Learn more about Endoscopic Band Ligation at Memorial Hospital DefinitionReasons for ProcedurePossible ComplicationsWhat to ExpectCall Your Doctorrevision ...
Endoscopic Band Ligation | Trident Health System
Learn more about Endoscopic Band Ligation at Trident Health System DefinitionReasons for ProcedurePossible ComplicationsWhat to ExpectCall Your Doctorrevision ...
Understanding Minor Rectal Bleeding - Gastroenterologist Hialeah, Coral Gables, Miami, Pembroke Pines, FL
Medical treatment of hemorrhoids includes treatment of any underlying constipation, taking warm baths and applying an over-the-counter cream or suppository that may contain hydrocortisone. If medical treatment fails there are a number of ways to reduce the size or eliminate internal hemorrhoids. Each method varies in its success rate, risks and recovery time. Your doctor will discuss these options with you. Rubber band ligation is the most common outpatient procedure for hemorrhoids in the United States. It involves placing rubber bands around the base of an internal hemorrhoid to cut off its blood supply. This causes the hemorrhoid to shrink, and in a few days both the hemorrhoid and the rubber band fall off during a bowel movement. Possible complications include pain, bleeding and infection. After band ligation, your doctor may prescribe medications, including pain medication and stool softeners, before sending you home. Contact your doctor immediately if you notice severe pain, fever or ...
THE CATALYTIC ACTIVITY OF THE BLOOD IN RELATION TO (1) THE FUNCTIONAL SUFFICIENCY OF THE KIDNEYS; (2) PERITONITIS | Journal of...
1. The inhibiting action of urine on catalase depends to a great extent on its reaction. The urine of a nephritic has no greater inhibiting power than normal urine provided the reaction of urines tested is neutral. 2. The catalytic activity of a single rabbits blood is constant from day to day. 3. There is considerable variation in the catalytic action of the blood of different rabbits. 4. Following ligation of the ureters the catalytic activity of the blood gradually decreases; the tissues of an animal which has died as a result of ligation of the ureters show a decided decrease in catalytic activity, when compared with those of normal animals. 5. Following bilateral nephrectomy the same decline of the catalytic action of the blood is observed as after bilateral ligation of ureters. 6. Following unilateral nephrectomy the catalytic activity of the blood may temporarily fall and then rise above its previous level, or it may fall continuously. Wherever the continuous fall occurs the animal dies. ...
congenital fistulae are often difficult to treat because of multiple fistulae and the tendency for more fistulae to open up following ligation of the more obvious ones. Conservative treatment with compression stockings may be the best ...
Patent US5507797 - Slidable kit for endoscopic ligation - Google Patents
A slidable kit for endoscopic ligation of gastric and esophageal varices comprises an endoscope-inserting cylinder and a ligating device coaxially and integrally secured to the endoscope-inserting cylinder, where the ligating device is in such a structure that a slide cylinder having an annular rib on the outer periphery at the rearward end is inserted into an outer cylinder having an annular rib on the inner periphery at the forward end; an inner cylinder is inserted into the slide cylinder; the outer cylinder and the inner cylinder are integrated together at the rearward ends and firmly secured to the forward end of the endoscope-inserting cylinder; an elastic O ring is mounted on the outer periphery at the forward end of the inner cylinder; a seal ring is provided at the rearward end of the slide cylinder, thereby keeping an annular space between the outer cylinder, the inner cylinder and the slide cylinder hermetic; the slide cylinder is slidable forwards by a liquid introduced into the annular
Mouse models of myocardial infarction: comparing permanent ligation and ischaemia-reperfusion. - Oxford Neuroscience
Myocardial infarction (MI) is a disease of major consequence in the modern world, causing permanent, irreversible damage to the heart. Survivors are at risk for developing further cardiovascular pathologies such as heart failure. Further study of MI injury is crucial to improve the understanding and treatment of the post-MI heart. The most commonly used model for MI in vivo is surgical ligation of the left anterior descending coronary artery (LAD). There are two predominant approaches: permanent ligation (PL), where the LAD is permanently occluded with a suture, or ischaemia-reperfusion (IR), where the LAD is temporarily occluded before removing the suture to restore blood flow and tissue reperfusion. PL results in the majority of the area at risk becoming infarcted, leading to significant apoptotic cell death and a large scar. Conversely, IR salvages some of the area at risk; thus, the scar is smaller and includes reperfusion injury, an additional, albeit smaller, second wave of necrotic damage. PL may
Unilateral Varicose Vein Ligation and Stripping - Tour My India
Get the best quote for unilateral varicose vein ligation and stripping from the best internationally accredited hospitals at your preferred locations.
Causes ofr thrombosed hemorrhoids - hemorrhoids for 6 months
Some patients develop IBS as a result of poor nutritional choices while others get IBS because of stress If you have irritable bowel syndrome, you should meet with a specialist to determine what is causing this condition. The alternation of extreme temperature causes the hemorrhoids to piles spotting during early pregnancy and disappear. Many Homeopathic ingredients are effective in reducing the inflammation and pain associated with hemorrhoids. Rubber band ligation is frequently recommended for individuals with first- or second-degree hemorrhoids and, in some circumstances, third-degree hemorrhoids.
Vitamin D|sub|3|/sub| attenuates cortical infarction induced by middle cerebral arterial ligation in rats - 指紋 - 臺北醫學大學
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Rapid DNA Ligation Kit | China-Mainland | Sigma-Aldrich
For the Rapid DNA Ligation Kit, we guarantee , 1 x 106 transformants per μg religated pUC18 vector (sticky end as well as blunt end), i.e., this means that only 2.96 pg of vector (1 molecule of pUC 18 = of 2,96 ag [attogram!]), have be to be religated to result in this yield in transformants. This rate of religation is easily achieved in 5 minutes with the Rapid DNA Ligation Kit.. Note: This also means that when ligation products are checked using an agarose gel, a substantial proportion of the vector DNA used will still show up â€žunligatedâ€œ. Considering the above mentioned molecular weight of pUC 18 this is is easily visualized.. The rate and kinetics of concatemer ligation in cloning with λ-vectors are totally different from plasmid ligation. For ligation of concatemers ligase has to connect linear fragments in the presence of high amounts of DNA. For plasmid cloning an insert must first be ligated to one end of the linearized plasmid-vector, and then the other end of the same ...
Cook Childrens Health Library - Endoscopic Band Ligation - Procedures - 199399
For this procedure, you will lie on your left side. If you are not intubated a mouthpiece will be placed to help keep your mouth open. An assistant will be in the room to monitor your breathing and heartbeat. You may also be given oxygen through your nose. A suction tube will be used to clear the saliva and other fluids from your mouth.. A lubricated endoscope will be placed into your mouth. It will be passed down your throat and into your esophagus. The scope will have a small light and a camera. The doctor will watch the images on a video monitor. Air will be passed through the scope to help your doctor see your esophagus. The doctor will be able to locate the enlarged vein.. Instruments will be passed through the scope. The enlarged tissue will be sucked into the devices chamber. One or more bands will be placed around the tissue to clamp off the blood supply. ...
Patent US5383882 - Ligature and ligature applying endoscopic instrument - Google Patents
An endoscopic instrument for the application of ligature binders and a ligature are described. A base body and an actuation part of a handle part are longitudinally displaceable relative to each other against an elastic force within a fixed range. Connected to the actuation part is an actuation rod at whose distal end a first ratchet heat is secured. A shaft, firmly connected to the base body, carries a second ratchet head at its distal end. With the help of the ratchet heads, which are matched to the toothed end zone of a ligature binder, a ligature can be drawn together. A ligature whose end zone has already been inserted into the endoscopic instrument prior to the operation can be brought into a closed shape with the help of a constricted zone in its binding part and of a longitudinal slot in its headpiece.
Peripheral and spinal 5-HT receptors participate in cholestatic itch and antinociception induced by bile duct ligation in rats....
Sigma-Aldrich offers abstracts and full-text articles by [Bin Tian, Xue-Long Wang, Ya Huang, Li-Hua Chen, Ruo-Xiao Cheng, Feng-Ming Zhou, Ran Guo, Jun-Cheng Li, Tong Liu].
DNA Ligation Kit | TA Cloning Kit | Ligation Mix
DNA Ligation Kit, Mighty Mix, is a one-solution premix ligation reagent that offers high efficiency ligation in as little as 5 minutes. Small reaction volumes and efficient for blunt end and TA cloning.
Search Results for Medical and Health Sciences | AKJournals
Bevezetés: A májtumorok reszekálhatóságának egyetlen biológiai feltétele a májreszekció után megmaradó, a májműködést még biztosítani képes elégséges májszövetmennyiség (FLR). A műtét után visszamaradó májszövet tömege még a műtét előtt megnöveszthető vena portae okklúziós eljárásokkal. A legújabb ilyen eljárás az Associating Liver Partition and Portal vein ligation for Staged hepatectomy (ALPPS), mely a legrövidebb idő alatt a legjelentősebb indukált hipertrófiát biztosítja. A műtét morbiditása és mortalitása az eljárás bevezetése idején azonban elfogadhatatlanul magas volt. Célkitűzés: A szövődmények csökkentése a betegszelekció pontosításával és a műtéti technika módosításával. Módszer: Műtéttechnikai módosításként a teljes mobilizálás technika helyett klinikánkon ún. no touch technikát dolgoztunk ki. Klinikánk betegadatait nemzetközi regiszterbe kapcsoltuk, melyben gyűjtjük a demográfiai ...
Items where Author is Kovács, Tibor - Repository of the Academys Library
Kovács, Tibor and Billes, Viktor and Komlós, Marcell and Hotzi, Bernadette and Manzéger, Anna and Tarnóci, Anna and Papp, Diána and Szikszai, Fanni and Szinyákovics, Janka and Ákos Rácz, Ákos and Noszál, Béla and Veszelka, Szilvia and Walter, Fruzsina R. and Deli, Mária A. and Hackler Jr., László and Alföldi, Róbert and Huzian, Orsolya and Puskás, László G. and Liliom, Hanna and Tárnok, Krisztián and Schlett, Katalin and Borsy, Adrienn and Welker, Ervin and Kovács, Attila L. and Pádár, Zsolt and Erdős, Attila and Légrádi, Ádám and Bjelik, Annamária and Gulya, Károly and Gulyás, Balázs and Vellai, Tibor (2017) The small molecule AUTEN-99 (autophagy enhancer-99) prevents the progression of neurodegenerative symptoms. SCIENTIFIC REPORTS. pp. 1-17. ISSN 2045-2322 Kovács, Tibor and Máthé, Domokos and Fülöp, András and Jemnitz, Katalin and Bátai-Konczos, Attila (2017) Functional shift with maintained regenerative potential following portal vein ligation. ...
HELP WANTED: Trouble with ligation
In the past I have had two main problems with failed ligation reactions. The first appeared to involve a seasonal decrease in the quality of MilliQ water (associated with filters which were not changed often enough). This wouldnt appear to have affected you, as your phosphorylated vector was religated. The second problem I have encountered, involves over CIPing your vector. Cheaper preparations of phosphatase have traces of exonuclease activity, these will nibble the ends of DNA. The more common problem is the carry over of CIP into the ligation reaction. This effectively removes the phosphates from your insert, thus no ligation and no transformants. I routinely use no more than 1unit CIP/pmole DNA ends (routinely 0.2unit/pmoleDNA). I heat inactivate the CIP (65C/20mins) in the presence of 5mM EDTA, phenol extract and gel purify the linearised vector. This method maybe a bit over the top, but I have had no problems cloning when my vector is purified in this way. Alternatively, one can use ...
Rovner ML- Metal Platinum P-1RL (AS) Ligature with Capsule
The Rovner P-1RL Platinum Alto Saxophone Ligature is a piece of metal ligature that includes a Rovner Cap. Producing a pure, dark, tone this ligature creates a ringing bell-like sound and enables effortless playing with an evenness of scale, smoothing out the difficult transitions between registers. Providing you with superior control for a secure, centered tone and flawless intonation, the Platinum Ligature allows for unprecedented reed-gripping capabilities.
Information on Tubal Ligation on MedicineNet.com
Some women complain of serious medical problems after having their tubes tied. They even have a name for it: post-tubal ligation syndrome. But doctors have yet to find anything wrong.
β3-Integrin Mediates Smooth Muscle Cell Accumulation in Neointima After Carotid Ligation in Mice | Circulation
Thank you for your interest in spreading the word on Circulation.. NOTE: We only request your email address so that the person you are recommending the page to knows that you wanted them to see it, and that it is not junk mail. We do not capture any email address. ...
Shorten the Ligation Time for the pGEM-T Vector Systems
This article, describes conditions for shortening ligation time when cloning into the pGEM-T Vector Systems. A 15-minute ligation gave approximately 50% transformants by blue/white selection with further improvements when BSA was added.
Can you become pregnant after a tubular ligation - Answers on HealthTap
Doctors give trusted, helpful answers on causes, diagnosis, symptoms, treatment, and more: Dr. Schomberg-Klaiss on can you become pregnant after a tubular ligation: There is a low failure rate for tubal ligation - so it is potentially possible.
Collections: Images from the History of Medicine (IHM) / Languages: English / Subjects: Ligation and Patients / Genre:...
Start Over You searched for: Collections Images from the History of Medicine (IHM) ✖Remove constraint Collections: Images from the History of Medicine (IHM) Languages English ✖Remove constraint Languages: English Subjects Ligation ✖Remove constraint Subjects: Ligation Subjects Patients ✖Remove constraint Subjects: Patients Genre Photographs ✖Remove constraint Genre: Photographs Dates by Range 1900-1949 ✖Remove constraint Dates by Range: 1900-1949 Dates by Range 1950-1999 ✖Remove constraint Dates by Range: 1950-1999 ...
Ligation...is it really effective?
You know the best explanation to that guys is that....whatever plans that we are going to make and this plan is not Gods plan, then, it will not really happen.No matter how many times your tubes are being tied, if its Gods will that you will get pregnant nobody and nothing can stop Gods plan even tubal ligation.. ...
Reagents for Molecular Cloning: Ligation and In-Fusion Kits
Your success starts with experimental design, and each decision has an impact. We offer a comprehensive supply of best-in-class reagents to fit every cloning project-choose the kit thats right for you:. ...
Look up ligation in Wiktionary, the free dictionary. Ligation may refer to: Ligation (molecular biology), the covalent linking ... the production of peptides from amino acids Tubal ligation, a method of female sterilization Rubber band ligation, a treatment ... a method of attaching the archwires to the brackets KAHA Ligation Ligation-independent cloning Typographic ligature forming ... This disambiguation page lists articles associated with the title Ligation. If an internal link led you here, you may wish to ...
See Tubal ligation methods below.) If the patient chooses a postpartum tubal ligation, the procedure will further depend on the ... Partial tubal ligation or full salpingectomy (a tubal ligation method that relies upon the physical removal of the fallopian ... Tubal ligation is considered a permanent method of sterilization and birth control. Female sterilization through tubal ligation ... Since most forms of tubal ligation require abdominal surgery under regional or general anesthesia, tubal ligation is also ...
It is an alternative to the Native Chemical Ligation (NCL). KAHA Ligation was developed by Jeffrey W. Bode group at ETH Zürich ... The first reported protein synthesized by KAHA ligation was human GLP-1 (7-36). Since then, a variety of small proteins (up to ... The most commonly used N-terminal hydroxylamine is the 5-oxaproline, which results in a homoserine residue after ligation and O ... The α-Ketoacid-Hydroxylamine (KAHA) Amide-Forming Ligation is a chemical reaction that is used to join two unprotected ...
In native chemical ligation, an unprotected peptide-thioester reacts with a Cys-peptide to give a ligation product with a ... The Staudinger ligation continues to be developed and has not yet found widespread use. Ser/Thr ligation (STL) was introduced ... The original chemical ligation methods involved the formation of a non-native bond at the ligation site. Subsequently, native ... Native chemical ligation relies on the presence of a cysteine residue at the ligation site. Methods using removable auxiliary ...
... (LIC) is a form of molecular cloning that is able to be performed without the use of restriction ... "Ligation Independent Cloning (LIC)". New England BioLabs (NEB). Retrieved 15 January 2016. "Get Your Clone 90% Of The Time with ... Haun, RS; Serventi, IM; Moss, J (1992). "Rapid, reliable ligation-independent cloning of PCR products using modified plasmid ...
Native chemical ligation
Intein KAHA Ligation Peptide synthesis Protein synthesis SEA Native Peptide Ligation Dawson, P. E.; Muir, T. W.; Clark-Lewis, I ... Alternatively, thiol-containing ligation auxiliaries can be used that mimic an N-terminal cysteine for the ligation reaction, ... Native Chemical Ligation (NCL) is an important extension of the chemical ligation concept for constructing a larger polypeptide ... Native chemical ligation of this kind using a recombinant polypeptide segment is known as Expressed Protein Ligation. Similarly ...
Sequencing by ligation
... is a DNA sequencing method that uses the enzyme DNA ligase to identify the nucleotide present at a given ... Sequencing by ligation relies upon the sensitivity of DNA ligase for base-pairing mismatches. The target molecule to be ... Sequencing by ligation can proceed in either direction (either 5'-3' or 3'-5') depending on which end of the probe ... This sequencing by ligation method has been reported to have problems sequencing palindromic sequences. Sequencing by ...
Rubber band ligation
"Haemorrhoidal artery ligation versus rubber band ligation for the management of symptomatic second-degree and third-degree ... The CRH O'Regan ligation system also eliminates the use of forceps. It is much more expensive on a per-case basis than the ... Rubber band ligation is a popular procedure for the treatment of hemorrhoids, as it involves a much lower risk of pain than ... With rubber band ligation, a small band is applied to the base of the hemorrhoid, stopping the blood supply to the hemorrhoidal ...
Ligation (molecular biology)
TA ligation is therefore a form of sticky end ligation. Blunt-ended vectors may be turned into vector for TA ligation with ... Ligation is complicated by the fact that the desired ligation products for most ligation reactions should be between two ... Blunt-end ligation is much less efficient than sticky end ligation, so a higher concentration of ligase is used in blunt-end ... For the ligation reaction to proceed efficiently, the ends should be stably annealed, and in ligation experiments, the Tm of ...
Proximity ligation assay
In situ proximity ligation assays (isPLA) has been applied to antibody validation in human tissues with various advantages over ... Proximity ligation assay (in situ PLA) is a technology that extends the capabilities of traditional immunoassays to include ... Roussis IM, Guille M, Myers FA, Scarlett GP (2016). "RNA Whole-Mount In situ Hybridisation Proximity Ligation Assay (rISH-PLA ... Leuchowius KJ, Weibrecht I, Landegren U, Gedda L, Söderberg O (October 2009). "Flow cytometric in situ proximity ligation ...
SEA Native Peptide Ligation
The overall process of SEA native peptide ligation involves first an N,S-acyl shift for in in situ formation of a peptide ... SEA is an abbreviation of bis(2-sulfanylethyl)amido (Scheme 1). SEA ligation involves the reaction of a peptide featuring a C- ... The first peer reviewed publication describing SEA native peptide ligation was published in Organic Letters by Melnyk, O. et al ... Ollivier, N.; Dheur, J.; Mhidia, R.; Blanpain, A.; Melnyk, O., Bis(2-sulfanylethyl)amino native peptide ligation. Org. Lett. ...
Multiplex ligation-dependent probe amplification
... (MLPA) is a variation of the multiplex polymerase chain reaction that permits ... Multiplex ligation-dependent probe amplification was invented by Jan Schouten, a Dutch scientist. The method was first ... "Multiplex Ligation-dependent Probe Amplification (MLPA)". Bitesize Bio. 2018-12-27. Retrieved 2021-06-07. Yau SC, Bobrow M, ... X and Y based on multiplex ligation-dependent probe amplification (MLPA)". Eur. J. Hum. Genet. 13 (2): 171-175. doi:10.1038/sj. ...
Distal Revascularization and Interval Ligation
... (DRIL) is a surgical method of treating vascular access steal syndrome. DRIL was ... by arteriovenous fistula with distal artery ligation and revascularization". J Vasc Surg. 7 (6): 770-3. doi:10.1016/0741-5214( ...
Split-intein circular ligation of peptides and proteins
... (SICLOPPS) is a biotechnology technique that permits the creation of ...
"Tubal Ligation". Johns Hopkins Medicine. "Vasectomy". Johns Hopkins Medicine. Lin, Luo; Wu Shi-Zhong; Zhu Changmin; Fan Qifu; ... There are multiple ways of having sterilization done, but the two that are used most frequently are tubal ligation for women ... This method is considered more effective than tubal ligation, as there is no chance of tubal reconnection or clip failure, and ... A 1996 Chinese study found that "risk for depression was 2.34 times greater after tubal ligation, and 3.97 times greater after ...
Molecular-weight size marker
One such method employs the technique of partial ligation. DNA ligation is the process by which linear DNA pieces are connected ... Bowen, R (20 October 1999). "DNA Ligation". Biotechnology and Genetic Engineering. Retrieved 12 November 2013. Lan, Vo Thi ...
1959 Aug;66:640-8. Tubal ligation. Green GH. N Z Med J. 1958 Oct;57(321):470-7. Foetal renal hypoplasia and the origin of ...
In traceless Staudinger ligation, the organophosphorus group dissociates giving a phosphorus-free bioconjugate. Gololobov, Y. G ... In classical Staudinger ligation, the organophosphorus compound becomes incorporated into the peptide. Typically, appended to ... Of interest in chemical biology is the Staudinger ligation, which has been called one of the most important bioconjugation ... Two versions of the Staudinger ligation have been developed. Both begin with the classic iminophosphorane reaction. ...
... the tetrazine ligation, the isocyanide-based click reaction, and most recently, the quadricyclane ligation. The use of ... The Staudinger ligation has been used in both live cells and live mice. The azide can act as a soft electrophile that prefers ... The Staudinger ligation is a reaction developed by the Bertozzi group in 2000 that is based on the classic Staudinger reaction ... The quadricyclane ligation utilizes a highly strained quadricyclane to undergo [2+2+2] cycloaddition with π systems. ...
Glal hydrolysis and Ligation Adapter Dependent PCR assay (GLAD-PCR assay) is the novel method to determine R(5mC)GY sites ... The universal adapter ligation. As an adapter an oligonucleotide duplex 5'-CCTGCTCTTTCATCG-3'/3'-pGGACGAGAAAGTAGCp-5' is used, ...
In native chemical ligation a peptide C-terminal thioester reacts with a second peptide that has a cysteine residue at its N- ... Peptide and protein ligation. 25 (18): 4953-4965. doi:10.1016/j.bmc.2017.05.020. PMID 28705433. Bunker RD, Mandal K, Bashiri G ... Native chemical ligation of unprotected peptide segments is used to prepare the protein's polypeptide chain, which is then ... The hydrazide is stable to native chemical ligation reaction conditions, and can be converted in situ to a reactive peptide- ...
ISBN 978-4-431-55823-1. Nuijens T, Schmidt M (2019). Enzyme-mediated ligation methods. Humana, New York, NY. ISBN 978-1-4939- ...
Nikolay P. Serdev
Their suturing ligation without excision]". Khirurgiia (in Bulgarian). 43 (4): 65-8. PMID 2097429. Serdev N (1991). "[Free ...
Endonucleolytic cleavage and ligation "Ptc1". Saccharomyces Genome Database. SGD Project. Retrieved 21 March 2014. Malleshaiah ...
9q34.3 deletion syndrome
"Multiplex Ligation-Dependent Probe Amplification". The Journal of Molecular Diagnostics. 8 (4): 433-443. doi:10.2353/jmoldx. ... multiplex ligation-dependent probe amplification (MLPA), array comparative genomic hybridization (aCGH), and EHMT1 sequencing. ...
Additional ligation of the intersphincteric fistula tract did not improve the outcome after endorectal advancement flap. ... ligation of intersphincteric fistula tract) procedure. LIFT procedure is based on secure closure of the internal opening and ... the ligation of intersphincteric fistula tract". J Med Assoc Thai. 90 (3): 581-6. PMID 17427539. van Onkelen, RS; Gosselink, MP ... ligation of intersphincteric tract close to the internal opening and removal of intersphincteric tract, scraping out all ...
Nablus mask-like facial syndrome
"Multiplex Ligation-dependent Probe Amplification (MLPA)". bitesizebio.com. 2018-12-27. Retrieved 2022-03-24. "Nablus mask-like ... and multiplex ligation-dependent probe amplification (MLPA) based techniques. Post-natal diagnoses are more common, but ...
Laura L. Kiessling
He, Ye; Hinklin, Ronald; Chang, Jiyoung; Kiessling, Laura (2004). "Stereoselective N-Glycosylation by Staudinger Ligation". ...
Hypogastric artery ligation. Bilateral ligation of the internal iliac arteries reduces the pulse pressure of blood flowing to ... with or without ligation of the tubo-ovarian vessels. Ligation of the uterine and utero-ovarian arteries can decrease uterine ... Shahin AY, Farghaly TA, Mohamed SA, Shokry M, Abd-El-Aal DE, Youssef MA (March 2010). "Bilateral uterine artery ligation plus B ... Clark SL, Phelan JP, Yeh S, Bruce SR, Paul RH (1985). "Hypogastric Artery Ligation for Obstetric Hemorrhage". Obstetrics & ...
Longer lengths can be accessed by using ligation approaches such as native chemical ligation, where two shorter fully ... The most common form of native chemical ligation uses a peptide thioester that reacts with a terminal cysteine residue. Other ... Muir TW, Sondhi D, Cole PA (June 1998). "Expressed protein ligation: a general method for protein engineering". Proceedings of ... Chow HY, Zhang Y, Matheson E, Li X (September 2019). "Ligation Technologies for the Synthesis of Cyclic Peptides". Chemical ...
Ligation - Wikipedia
Look up ligation in Wiktionary, the free dictionary. Ligation may refer to: Ligation (molecular biology), the covalent linking ... the production of peptides from amino acids Tubal ligation, a method of female sterilization Rubber band ligation, a treatment ... a method of attaching the archwires to the brackets KAHA Ligation Ligation-independent cloning Typographic ligature forming ... This disambiguation page lists articles associated with the title Ligation. If an internal link led you here, you may wish to ...
Tubal ligation: MedlinePlus Medical Encyclopedia
Tubal ligation is surgery to close a womans fallopian tubes. (It is sometimes called tying the tubes.) The fallopian tubes ... Tubal ligation can also be done right after you have a baby through a small cut in the navel. It can also be done during a C- ... Tubal ligation may be recommended for adult women who are sure they do not want to get pregnant in the future. The benefits of ... Tubal ligation is considered a permanent form of birth control. It is NOT recommended as a short-term method or one that can be ...
Rubber-Band Ligation of Hemorrhoids: Background, Indications, Contraindications
Background Hemorrhoid ligation is one of the most common outpatient treatments available for patients with hemorrhoids. In this ... encoded search term (Rubber-Band Ligation of Hemorrhoids) and Rubber-Band Ligation of Hemorrhoids What to Read Next on Medscape ... The 1-year recurrence rate was 30% for HAL and 49% for rubber-band ligation; however, when multiple band ligations were ... The HubBLe Trial: haemorrhoidal artery ligation (HAL) versus rubber band ligation (RBL) for symptomatic second- and third- ...
Ligation Accessories | Medtronic Animal Health
Possible Complications After a Tubal Ligation
Learn about the safety of the tubal ligation procedure, including complications such as infection and ectopic pregnancy. ... Is Tubal Ligation Safe? Death during the procedure is extremely rare, occurring in about 1 to 2 out of 100,000 tubal ligations ... The overall complication rate associated with laparoscopic tubal ligation is approximately 0.9 to 1.6 per 100 tubal ligation ... Can scar tissue from tubal ligation cause pain years later? It is possible, but not very common to have scar tissue after a ...
Articles, tagged with "tubal ligation" - 3
Tubal Ligation Reversal: Purpose, Procedure, Risks & Success
Tubal ligation reversal is a surgery that reopens your fallopian tubes after tubal ligation. It may be an option to restore ... Tubal Ligation Reversal Tubal ligation reversal is a surgery that reopens your fallopian tubes after tubal ligation (having ... Tubal ligation reversal is surgery to undo a tubal ligation. A tubal ligation reversal reconnects or reopens your tubes, ... Tubal ligation reversal may be an option if youve had a tubal ligation but would now like to try for a baby. Before proceeding ...
Ligation Protocol with T3 DNA Ligase (M0317) | NEB
Home Protocols Ligation Protocol with T3 DNA Ligase (M0317) Ligation Protocol with T3 DNA Ligase (M0317). *Set up the following ... Note that the table shows a ligation using a molar ratio of 1:3 vector to insert for the indicated DNA sizes.) Use ... NEBioCalculator® - Using the Ligation module This tutorial describes the use of the NEBioCalculator web tool to optimize the ... molar ratio between vector and insert DNA for use in a ligation reaction. ...
Tubal Ligation: Can My Tubes Come "Untied"? | HealthNews
700,000 tubal ligations are performed annually in America with few complications or failures, and there are reasons why your ... What is tubal ligation? Tubal ligation, or getting your tubes tied, is a form of permanent birth control that involves cutting ... Tubal ligation failure The chances of getting pregnant after a tubal ligation due to failure of the procedure are also ... Can you get pregnant after a tubal ligation? Some women may wonder if a tubal ligation is reversible or if there is a way to ...
A hybridization-based approach for quantitative and low-bias single-stranded DNA ligation
We show here that existing ssDNA ligation methods suffer from slow kinetics, poor yield, and severe nucleotide preference. To ... ligation is a crucial step in many biochemical assays. Efficient ways of carrying out this reaction, however, are lacking. ... This technique potentially can be applied in protocols that require ligation of ssDNA, including ligation-mediated polymerase ... A hybridization-based approach for quantitative and low-bias single-stranded DNA ligation Anal Biochem. 2013 Apr 15;435(2):181- ...
Reversing Tubal Ligation
The decision about which procedure to have will depend on the type of tubal ligation you had and if it can be reversed ( ... But before you pick up the phone to make an appointment with your doctor, you need to understand that the tubal ligation ... Overall, the average cost per delivery was $17,167 for IVF and $7,263 for tubal ligation. This study, however, was conducted in ... If you choose surgery to reverse the tubal ligation, you will require general anesthesia, which also carries some risks. Then ...
Female Sterilisation | Tubal ligation | Spire Norwich Hospital
Frontiers | Realizing serine/threonine ligation: scope and limitations and mechanistic implication thereof
Herein, we have fully investigated the compatibility of the serine/threonine ligation strategy for X-Ser/Thr ligation sites, ... Herein, we have fully investigated the compatibility of the STL strategy for X-Ser/Thr ligation sites, where X is any of the 20 ... Our studies have shown that 17 amino acids are suitable for ligation, while Asp, Glu, and Lys are not compatible. Among the ... Our studies have shown that 17 amino acids are suitable for ligation, while Asp, Glu, and Lys are not compatible. Among the ...
40-year-old died from botched IUD removal/tubal ligation surgery: lawsuit - LifeSite
Treatment of spontaneous tumours by temporary local ligation
Tubal Ligation Archives - Conscious Woman
Chances of Getting Pregnant After Tubal Ligation Reversal
Tubal ligation regret is very common.. If you are like most women and regret your tubal ligation you will seek advice from your ... Method of tubal ligation. The type of tubal ligation can has a big impact on your chances of getting pregnant after tubal ... Tubal ligation is reversible. The chances of getting pregnant after tubal ligation reversal are much better than you think. ... What are the chances of becoming pregnant after tubal ligation reversal? Is reversal of a tubal ligation even possible? ...
CPT® Code - Ligation Procedures on Arteries and Veins 37565-37785 - Codify by AAPC
Crossectomy or ligation
Tubal Ligation Reversal in Mexico Offers New Options
Tubal Reversal is a surgical procedure that helps restore fertility to women after a tubal ligation. If you wish to know more ... Cost of Tubal Ligation Reversal. A tubal ligation reversal procedure can cost much more than the original ligation process due ... Tubal Ligation Reversal in Mexico. Tubal ligation is a form of female sterilization.The procedure is performed by tying, ... Tubal Ligation Reversal in Mexico Offers New Options. Tubal Reversal in Mexico, Tubal Ligation Reversal, TLR, Price of Tubal ...
Tubal Ligation Surgery in Central NY | Permanent Birth Control
Tubal Ligation Recovery. After receiving your tubal ligation, you can expect to go home the same day. You should ask your ... After tubal ligation, pregnancy is extremely rare. If a sperm is able to fertilize an egg, however, it is possible for an ... Tubal ligation, or removal of tubes at Bassett Healthcare Network is performed in a hospital operating room. If you are having ... When a woman wants to avoid becoming pregnant in her future, she may consider tubal ligation - commonly known as getting your " ...
Proximity Ligation Assay - Technologies - Support - Abnova
Proximity Ligation Assay. The in situ proximity ligation assay is a powerful technology capable of detecting single protein ... Ligation to form a complete DNA circle 5. Rolling circle amplification 6. Add fluorescent probes to reveal protein protein ... Cytokine detection by antibody-based proximity ligation.. Gullberg M, Gustafsdottir SM, Schallmeiner E et al. Proc Natl Acad ... Protein detection using proximity-dependent DNA ligation assays.. Fredriksson S, Gullberg M, Jarvius J et al. Nat Biotechnol. ...
A new cyclam with an appended imidazole. The first biomimetic ligation of imidazole for axial π-interaction with metal ions -...
Element Biosciences Partners with Dovetail Genomics to Validate its Proximity Ligation-Based NGS Library Prep Solutions for...
Element Biosciences Partners with Dovetail Genomics to Validate its Proximity Ligation-Based NGS Library Prep Solutions for ... Their proximity ligation technology couples well with Elements improvements in sequencing accuracy, lower optical duplication ... "We are thrilled to partner with Element Biosciences to add Dovetail Genomics cutting-edge proximity ligation technology to ... Element Biosciences Partners with Dovetail Genomics to Validate its Proximity Ligation-Based NGS Library Prep Solutions for ...
Catholic Hospitals Deny Tubal Ligations as "Intrinsically Evil" - LA Progressive
Chamorro are also Catholic-affiliated, she would need to travel 70 or even 160 miles to get her tubal ligation at the same time ... She and her husband decided their family would be complete with three children and she opted to have a tubal ligation-a ... Chamorro is our third client in the past several months who has requested a postpartum tubal ligation in a Catholic hospital ... Chamorro made the same decision about a quarter of American women make when they opt for birth control - tubal ligation ...
Symptoms Of Pregnancy After Tubal Ligation And Risks Involved
Is it possible to get pregnant after tubal ligation? This article can help you understand the odds, symptoms, and risks of ... 2. Tubal ligation and tubal implants; UPMC (2019). 3. Chances of Pregnancy After Tubal Ligation; Sutter Health (2018). 4. Akaba ... 9. Tubal Ligation Reversal; American College of Cardiology. 10. Tubal Ligation Reversal; UAB Medicine (2019). 11. Claire Hannum ... Tubal ligation is a medical sterilization (birth control) procedure for women. However, pregnancy after tubal ligation may ...
Tubal Ligation Reversal in Mexico Testimonial | Tubal Reversal Review
Why You Should Choose Lakeshore Surgical for Your Tubal Ligation Reversal - Lakeshore Tubal Reversal Center
Tubal Ligation Reversal | Catholic Health - The Right Way to Care
Most likely, their ligations either: removed a small section of the fallopian tubes, or placed clips or rings around the tubes ... For an ideal patient, tubal ligation reversal is 80-90% successful. Candidates for Surgery Women who make the best candidates ... Tubal ligation is a procedure that permanently ends a womans ability to have children. For women whose circumstances have ... Tubal ligation is a procedure that permanently ends a womans ability to have children. For women whose circumstances have ...
ReversalCecal ligation and punctureProcedureSterilizationSurgeryTypes of tubal ligationReverse the tubal ligationHysterectomyLaparoscopic tubal ligationMultiplexType of tubalPostpartum tubal ligationTubesNative chemicalRubber-band ligPolymerase chain reBand ligationPeptide ligationFallopian tubeSciatic nerveAmino acidsVasectomyTubal.orgUterusLigaseEctopic pregnancy20212022HemorrhoidsEssureVaricealPregnantProximityHypogastricNeuropathic painIndicationsExcisionAssaysSpinalRisksComplicationsInvolves2000OligosProceduresBilateralPrevent pregnancyPregnancy ratesPros andContraceptiveVector and insertElectroporationDevelopments
- IVF vs. tubal reversal is usually the comparison that is made when a woman wishes to untie tubes that have been blocked by tubal ligation. (articlealley.com)
- Tubal ligation reversal is a surgery that reopens your fallopian tubes after tubal ligation (having your tubes tied). (clevelandclinic.org)
- What is a tubal ligation reversal? (clevelandclinic.org)
- After tubal ligation, the only options for becoming pregnant is either tubal ligation reversal surgery or in vitro fertilization (IVF). (clevelandclinic.org)
- Tubal ligation reversal is surgery to undo a tubal ligation. (clevelandclinic.org)
- A tubal ligation reversal reconnects or reopens your tubes, creating a passageway for eggs and sperm to unite so that conception can occur. (clevelandclinic.org)
- Tubal ligation reversal is also called tubal reversal, tubal sterilization reversal and tubal reanastomosis. (clevelandclinic.org)
- Who's a good candidate for tubal ligation reversal? (clevelandclinic.org)
- You're a good candidate for tubal ligation reversal if there's a good chance you can become pregnant after surgery. (clevelandclinic.org)
- Irregular periods, uterine fibroids , scarring in your pelvis or endometriosis (a condition where the tissue lining your uterus is also present outside of your uterus) may reduce your chances for success after a tubal ligation reversal. (clevelandclinic.org)
- The type of tubal ligation you had will determine how successful tubal reversal will be. (clevelandclinic.org)
- What happens before tubal ligation reversal? (clevelandclinic.org)
- Your provider will assess the quantity and quality of your partner's sperm to determine your likelihood of pregnancy following a tubal ligation reversal. (clevelandclinic.org)
- Tubal ligation reversal surgery lasts about two to three hours and takes place in a hospital. (clevelandclinic.org)
- What are the chances of becoming pregnant after tubal ligation reversal? (tubal-reversal.net)
- Is reversal of a tubal ligation even possible? (tubal-reversal.net)
- The chances of getting pregnant after tubal ligation reversal are much better than you think. (tubal-reversal.net)
- Ironically IVF is more expensive and often less successful than tubal ligation reversal surgery. (tubal-reversal.net)
- You may be amazed to learn this but the National US Society of infertility doctors actually recommends considering tubal ligation reversal surgery for women who want more babies after tubal ligation! (tubal-reversal.net)
- A tubal ligation reversal procedure is considered an outpatient or same-day surgery type procedure. (placidway.com)
- The ligation reversal procedure is done through microsurgical techniques that open and reconnect fallopian tube segments. (placidway.com)
- A tubal ligation reversal procedure can cost much more than the original ligation process due to its complexity. (placidway.com)
- In the U.S., the cost of a tubal ligation reversal averages $6,000 and may not include consultations, lab work, or facility and equipment or other fees such as anesthesia. (placidway.com)
- Written review of tubal ligation reversal in Mexico by Heidi Lopez-hernandez. (medicaltourismco.com)
- For an ideal patient, tubal ligation reversal is 80-90% successful. (chsbuffalo.org)
- Dr. Morice performs tubal ligation reversal surgery in Morgan City, Louisiana. (mybabydoc.com)
- The 5 selected recipients will receive free fertility services that may include IVF, a tubal ligation reversal surgery, and a donor egg IVF cycle. (californianewswire.com)
Cecal ligation and puncture2
- The objective of this study was to identify biomarkers of sepsis-induced disseminated intravascular coagulation (DIC) among platelet-derived factors using biotin label-based custom protein microarray technology in a mouse cecal ligation and puncture (CLP) model. (biomedcentral.com)
- Dramatically, within 40 hours following cecal ligation and puncture all mice engineered so that they did not express Nrf2 had died. (pharmiweb.com)
- A number of prospective studies have found rubber-band ligation to be a simple, safe, and effective method for treating symptomatic first-, second-, and third-degree hemorrhoids as an outpatient procedure with significant improvement in quality of life. (medscape.com)
- Death during the procedure is extremely rare, occurring in about 1 to 2 out of 100,000 tubal ligations. (verywellhealth.com)
- Your tubal ligation procedure. (clevelandclinic.org)
- Two newer forms of tubal ligation include the Essure™ method and the less common Adiana™ procedure. (prostate.net)
- Women who have a traditional tubal ligation are sterile immediately after the procedure. (prostate.net)
- Tubal ligation is a safe and effective surgical procedure used to prevent pregnancy and reduce the risk of certain health conditions. (healthnews.com)
- Statistics suggest that one out of 100 women will get pregnant within one year of having their tubal ligation procedure. (healthnews.com)
- After a woman has a tubal ligation, it is doubtful her tubes will spontaneously untie or reconnect, allowing pregnancy to occur as it may have before a sterilization procedure. (healthnews.com)
- But before you pick up the phone to make an appointment with your doctor, you need to understand that the tubal ligation procedure you had is intended to be a permanent form of sterilization. (healthywomen.org)
- Overall pregnancy rates for tubal ligation range between 55 and 85 percent, usually higher when the procedure is performed through an abdominal incision. (healthywomen.org)
- Nicole Lee Stein Caruso had a 40-minute medical procedure to remove her IUD and have a tubal ligation on April 13, 2011. (lifesitenews.com)
- Tubal ligation is a form of female sterilization.The procedure is performed by tying, cutting or otherwise blocking the fallopian tubes. (placidway.com)
- Tubal ligation is usually a permanent procedure that prevents unwanted pregnancy. (placidway.com)
- If you are having a C-section delivery, you can undergo tubal ligation, or have your "tubes tied," during the same procedure. (bassett.org)
- Medical advantages to performing the tubal ligation postpartum include the practical ease of the procedure and the fact that a woman doesn't need a separate surgery and anesthetic later. (laprogressive.com)
- This post will tell you about tubal ligation, including the risk factors and chances of pregnancy post-procedure. (momjunction.com)
- Tubal ligation is a procedure that permanently ends a woman's ability to have children. (chsbuffalo.org)
- Occlusion of the blood supply to internal hemorrhoids by means of ligation is a practically painless procedure which can produce a controllable graded effect ranging from immediate arrest of bleeding to destruction of the hemorrhoid. (americanjournalofsurgery.com)
- To our knowledge, this is the first report describing a residual PDA shunt that was detected again with TEE during the PAB procedure after ductal flow had been confirmed to have ceased after PDA ligation. (springer.com)
- Women showing a significantly greater probability of regret were those sterilized at a young age, those who had not themselves made the decision to undergo surgery , those for whom the sterilization was carried out up to the 45th day after childbirth and those who had acquired knowledge about contraceptive methods after the tubal ligation procedure . (bvsalud.org)
- Ligation of the internal iliac arteries may be indicated as a life-saving procedure in the control of severe pelvic hemorrhage occurring spontaneously or operatively (when noninvasive methods are not indicated or feasible). (medscape.com)
- Pelvic ischemia due to bilateral hypogastric artery ligation was once a fear, but it has been shown that little morbidity, either short-term or long-term, results if the procedure is performed appropriately. (medscape.com)
- The younger you are at the time of a tubal ligation, the more likely the sterilization is to fail. (verywellhealth.com)
- Essure® and Adiana® are sterilization devices previously marketed as non-surgical alternatives to tubal ligation. (clevelandclinic.org)
- Both of these sterilization procedures should be regarded as permanent, even though surgeons can perform vasectomy reversals and tubal ligation reversals. (prostate.net)
- Tubal ligation is one of the most common surgical sterilization procedures for women. (healthnews.com)
- The additional patient reported outcome (PRO data) which we are analyzing is the results of hormone and other lab tests which have been done for you prior and after your tubal ligation/sterilization. (tubal.org)
- Tubal.org presents information about negative side effects of "tubal ligation" and "female sterilization" known as "post tubal syndrome" (PTS), "post tubal ligation syndrome" (PTLS), and "post sterilization syndrome" (PSS). (tubal.org)
- A case-control study was carried out at a public teaching hospital in Recife, Pernambuco State , Brazil in 1997 to investigate risk factors among women who feel regret after undergoing sterilization through tubal ligation . (bvsalud.org)
- It is necessary to assess women 's psycho-socio- demographic profiles, their reasons for requesting tubal ligation and to advise the patient about family planning in order to reduce rates of post- sterilization regret . (bvsalud.org)
- Bilateral tubal ligation is a permanent surgical form of female sterilization. (smartparenting.com.ph)
- The doctor who failed to successfully perform tubal ligation as a means of sterilization on a woman has been ordered to pay all expenses for a child, born after the failed operation, until it reaches adulthood. (hurriyetdailynews.com)
- Tubal ligation (getting your "tubes tied") is a type of surgery. (medlineplus.gov)
- [ 8 , 9 ] Band ligation may also be considered for bleeding in severely anemic patients with fourth-degree hemorrhoids who are unfit for surgery. (medscape.com)
- If you have diabetes or a history of previous abdominal surgery, pelvic inflammatory disease, or lung disease, or are overweight, you may have a higher risk for problems after your tubal ligation. (verywellhealth.com)
- They'll ask about your pregnancy history, tubal ligation surgery and any other pelvic surgeries. (clevelandclinic.org)
- After tubal ligation, women usually spend several hours or longer at the hospital if the surgery was done as part of another surgery. (prostate.net)
- No tests are performed after a tubal ligation to determine the surgery has been successful. (prostate.net)
- If you choose surgery to reverse the tubal ligation, you will require general anesthesia, which also carries some risks. (healthywomen.org)
- Surgeons at Bassett Healthcare Network offer tubal ligation, or fallopian tube removal- a gynecologic surgery - as a permanent birth control option. (bassett.org)
- CONCLUSION: Spinal hyperbaric ropivacaine 22 mg with or without fentanyl 10 μg could be used for postpartum tubal ligation surgery. (duke.edu)
Types of tubal ligation2
- What types of tubal ligation can't be reversed? (clevelandclinic.org)
- Are there different types of tubal ligation? (healthnews.com)
Reverse the tubal ligation1
- However, some women change their minds at a later point in their lives and want to reverse the tubal ligation. (placidway.com)
- Unlike a hysterectomy, a tubal ligation does not affect your menstrual cycle. (healthnews.com)
- As you can see, there is a lot to consider before having a hysterectomy or tubal ligation since those procedures can affect your hormones. (hotzehwc.com)
- IMSEAR at SEARO: Post- tubal ligation hysterectomy. (who.int)
- Post- tubal ligation hysterectomy. (who.int)
- Gupta AS, Saha M, Pramanik A. Post- tubal ligation hysterectomy. (who.int)
- Hysterectomy and Tubal Ligation. (bvsalud.org)
Laparoscopic tubal ligation1
- The overall complication rate associated with laparoscopic tubal ligation is approximately 0.9 to 1.6 per 100 tubal ligation procedures. (verywellhealth.com)
- Methods We evaluated promoter hypermethylation profiles of 22 common tumour suppressor genes in 108 NPCs using methylation-specific multiplex ligation-dependent probe amplification. (bmj.com)
- Multiplex ligation-dependent probe amplification can be used to detect changes in targeted genes. (medscape.com)
- Utility of multiplex ligation-dependent probe amplification (MLPA) for hemophilia mutation screening. (cdc.gov)
Type of tubal1
- A type of tubal ligation that involves applying an electrical current to the fallopian tube to cauterize (burn) different sections of the tube. (healthnews.com)
Postpartum tubal ligation4
- Some of those doctors have admitting privileges at Dignity Health hospitals and are being prevented from performing postpartum tubal ligation for their patients. (laprogressive.com)
- Minimum effective dose of spinal ropivacaine with and without fentanyl for postpartum tubal ligation. (duke.edu)
- BACKGROUND: Ropivacaine may be the ideal spinal anesthetic for postpartum tubal ligation due to its medium duration of action, low incidence of side effects and possibly reduced post-anesthetic care unit (PACU) stay. (duke.edu)
- METHODS: Two prospective up-down sequential allocation studies were performed using hyperbaric spinal ropivacaine via a combined spinal-epidural anesthetic technique for patients undergoing postpartum tubal ligation. (duke.edu)
- Tubal ligation, also known as a tubectomy or as "getting one's tubes tied ," is a permanent method of birth control . (verywellhealth.com)
- Tubal ligation (sometimes called "having your tubes tied") is an operation that prevents pregnancy by tying, cutting or burning your fallopian tubes . (clevelandclinic.org)
- A tubal ligation blocks your fallopian tubes so that an egg and a sperm can't meet. (clevelandclinic.org)
- Tubal ligation procedures where your healthcare provider uses clips or rings to tie your tubes are easier to reverse than procedures that involve burning your fallopian tubes (electrocautery). (clevelandclinic.org)
- The traditional tubal ligation, also known as "having your tubes tied," involves cauterizing or clamping the fallopian tubes to prevent pregnancy. (prostate.net)
- A tubal ligation stops eggs from reaching the uterus for fertilization and sperm from traveling up the fallopian tubes to the eggs. (prostate.net)
- After a non-surgical tubal ligation (Essure or Adiana), a woman may still be fertile for about three months, which is the amount of time it takes for the body to form a barrier in the fallopian tubes. (prostate.net)
- Tubal ligation, or getting your tubes tied, is a form of permanent birth control that involves cutting, tying, or permanently blocking the fallopian tubes. (healthnews.com)
- When a woman wants to avoid becoming pregnant in her future, she may consider tubal ligation - commonly known as getting your "tubes tied. (bassett.org)
- Tubal ligation, or removal of tubes at Bassett Healthcare Network is performed in a hospital operating room. (bassett.org)
- She and her husband decided their family would be complete with three children and she opted to have a tubal ligation-a permanent form of birth control commonly referred to as "getting your tubes tied. (laprogressive.com)
- It is a combination of two words where 'tubal' means the 'fallopian tubes' consisting of the eggs and 'ligation' refers to 'binding up. (momjunction.com)
- Tubal ligation is one of such decisions in which you get your tubes tied as a permanent birth control. (mybabydoc.com)
- The woman originally thought to have surgical tubal ligation (known as having tubes tied), but her physician assured her that Essure was a less invasive and generally preferable form of permanent birth control. (yourlawyer.com)
- We adopted the native chemical ligation (NCL) method between a C-terminal thioester fragment and an N-terminal Cys fragment. (upenn.edu)
- In rubber-band ligation, a rubber band is applied to the base of a hemorrhoid to hamper the blood supply to the hemorrhoidal mass. (medscape.com)
- [ 1 ] and rubber-band ligation is one of the most common outpatient treatments available for these patients. (medscape.com)
- Rubber-band ligation is readily performed in an ambulatory setting. (medscape.com)
- A study by Hite et al found that the use of clopidogrel bisulfate did not increase bleeding complications in patients undergoing rubber-band ligation for symptomatic hemorrhoids. (medscape.com)
- Ambulatory treatments for hemorrhoids include injection sclerotherapy, rubber-band ligation, cryosurgery, infrared coagulation, and ultrasonic Doppler-guided transanal hemorrhoidal artery ligation (HAL). (medscape.com)
- Also known as "banding," rubber band ligation treats bleeding, protruding, internal hemorrhoids by cutting off the blood supply and circulation to the hemorrhoid. (gastrohealthpartners.com)
Polymerase chain re2
- This technique potentially can be applied in protocols that require ligation of ssDNA, including ligation-mediated polymerase chain reaction (LMPCR) and complementary DNA (cDNA) library construction. (nih.gov)
- Analyses by IS 6110 restriction fragment length polymorphism (IS 6110 RFLP), ligation-mediated polymerase chain reaction (PCR), and spoligotyping were carried out by using standard protocols ( 7 - 9 ). (cdc.gov)
- Oesophageal variceal band ligation, being the best modality for controlling variceal bleeding has not been common practice in West Africa, a region recognized to have a high prevalence of liver diseases. (ajol.info)
- We present a case of band ligation of the varices of a patient that presented to our hospital with variceal haemorrhage, secondary to liver cirrhosis. (ajol.info)
- Conclusion: We present a case of successful variceal band ligation of a cirrhotic with extensive oesophageal varices presenting in a resource- constraint medical setting. (ajol.info)
- Chemical methods enabling merger of two fully side chain unprotected peptide segments at the termini, termed peptide ligation, provide access to complex, long peptides and even proteins beyond the limit of solid phase peptide synthesis (SPPS) ( Kent, 2009 ). (frontiersin.org)
- The realization of NCL lies in the super nucleophilicity of the N-terminal cysteine to mediate a chemoselective peptide ligation, thereby enabling merger of two side chain unprotected peptide segments with the generation of the natural Xaa-Cys at the ligation site. (frontiersin.org)
- our ligation strategy directly uses the natural serine or threonine residue at the N-terminus to mediate peptide ligation. (frontiersin.org)
- Our novel peptide ligation exhibits outstanding chemo- and regio-selectivity for proteinogenic peptides, tolerating all twenty proteinogenic sidechain residues. (findaphd.com)
- CPL demonstrates robust, easy-to-synthesize, stable nitriles can replace difficult-to-synthesize, unstable thioesters in peptide ligation. (findaphd.com)
- It was also found about one-third of the pregnancies resulted in ectopic (pregnancy taking place inside the fallopian tube instead of the uterus) following a tubal ligation (3) . (momjunction.com)
- Herein, we have fully investigated the compatibility of the STL strategy for X-Ser/Thr ligation sites, where X is any of the 20 naturally occurring amino acids. (frontiersin.org)
- Our studies have shown that 17 amino acids are suitable for ligation, while Asp, Glu, and Lys are not compatible. (frontiersin.org)
- Among the working 17 C-terminal amino acids, the retarded reaction resulted from the bulky β-branched amino acid (Thr, Val, and Ile) is not seen under the current ligation condition. (frontiersin.org)
- To remove the constraint of a Cys at the ligation site, we explored traceless ligation methods that desulfurized Cys into Ala, or β- and γ- thiol analogs into native amino acids after ligation in the presence of thioamides. (upenn.edu)
- Vasectomy vs tubal ligation for women is the permanent birth control choice that faces many couples. (prostate.net)
- Vasectomy vs tubal ligation for women, what's the story? (prostate.net)
- For couples who are deciding between vasectomy vs tubal ligation for women, it is helpful to compare the procedures in terms of cost, risks and complications, and recovery. (prostate.net)
- Overall, a vasectomy is safer, has a faster recovery period, a higher success rate, and is less expensive than tubal ligation. (prostate.net)
- The cost of vasectomy is much less than a tubal ligation. (prostate.net)
- Disclaimer: Diagnosis of health/medical conditions are not made at this web site, by the Coalition for Post Tubal Ligation Women (CPTwomen), tubal.org, or by any of its associates. (tubal.org)
- Tubal ligation prevents eggs from reaching the uterus. (placidway.com)
- However, what if you just had your uterus alone removed, or if you had a tubal ligation? (hotzehwc.com)
- Our method uses a hairpin DNA to hybridize to any incoming acceptor ssDNA with low bias, with ligation of these strands mediated by T4 DNA ligase. (nih.gov)
- Ectopic pregnancy is one of the risks associated with tubal ligation, even though the chances of pregnancy itself are rare. (momjunction.com)
- Therefore, if you have had a tubal ligation, get checked for ectopic pregnancy if you experience amenorrhoea or lower abdominal pain (4) . (momjunction.com)
- Uneétude descriptive, transversale portant sur les patientes traitées en radiothérapie à l'Hôpital Général de Douala pour cancer du col de l'utérus a été réalisée d'octobre 2020 à janvier 2021.Résultats. (bvsalud.org)
- The 2022 Global Forecast for Ligation Devices (2023-2028 Outlook), published by Barnes Reports, contains timely and accurate market statistics and forecasts on the market for over 50 countries. (barnesreports.com)
- According to the report published by Exactitude consultancy, the Ligation Devices Market is projected to attain 1,681.2 million through 2029, witnessing a CAGR of 6.2 % from 2022 to 2028. (articlemania.xyz)
- Hemorrhoid ligation is performed for first-degree, second-degree, and some cases of third-degree hemorrhoids when the patient complains of bleeding or prolapse of hemorrhoids. (medscape.com)
- Essure and Adiana have lower risks and complications than traditional tubal ligation because they do not involve general anesthesia or incisions, which reduces the risk of infection and speeds up recovery. (prostate.net)
- A Randomized-Controlled Trial of Endoscopic Treatment of Acute Esophageal Variceal Hemorrhage: N-Butyl-2-Cyanoacrylate Injection vs. Variceal Ligation. (irb.hr)
- OBJECTIVES: Acute esophageal variceal hemorrhage is still the most serious complication of portal hypertension and esophageal variceal ligation is endoscopic treatment of choice. (irb.hr)
- The aim of this prospective randomized trial was to compare the efficacy of N-butyl-2-cyanoacrylate injection and variceal ligation in the emergency endoscopic treatment of acute esophageal variceal hemorrhage in patients with portal hypertension and chronic liver disease. (irb.hr)
- METHODS: From January 2004 to December 2008 43 patients with endoscopy-proven acute esophageal variceal hemorrhage were randomly assigned to one of the two treatment groups: patients undergo endoscopic injection with N- butyl-2-cyanacrylate (n=22) and patients undergoing endoscopic variceal ligation (n=21). (irb.hr)
- Within two weeks after initial endoscopic treatment, prophylactic variceal ligation was started in on regular intervals until the varices were eradicated. (irb.hr)
- CONCLUSIONS: The efficacy of endoscopic injection therapy with N-butyl-2-cyanoacrylate to control acute esophageal variceal hemorrhage was not different to endoscopic variceal ligation and esophageal variceal rebleeding rate as well as mortality rate appears not different in those patients treated with N-butyl-2-cyanoacrylate injection than in patients treated with variceal ligation. (irb.hr)
- In the first year after a tubal ligation, it's estimated that fewer than 1 out of 100 women will get pregnant. (verywellhealth.com)
- It reverses a tubal ligation, allowing you to become pregnant again. (clevelandclinic.org)
- The attorney for the hospital where the doctor was working, meanwhile, demanded the dismissal of the case on grounds that there was a one in 1,000 probability in medical literature that a patient could become pregnant after a tubal ligation operation. (hurriyetdailynews.com)
- The in situ proximity ligation assay is a powerful technology capable of detecting single protein events such as protein protein interactions (e.g. protein dimerization) and modifications (e.g. protein phosphorylation) in tissue and cell samples prepared for microscopy. (abnova.com)
- Direct observation of individual endogenous protein complexes in situ by proximity ligation. (abnova.com)
- Cytokine detection by antibody-based proximity ligation. (abnova.com)
- Protein detection using proximity-dependent DNA ligation assays. (abnova.com)
- SAN DIEGO--( BUSINESS WIRE )-- Element Biosciences , Inc., developer of a new and disruptive DNA sequencing platform, today announced a partnership with Dovetail Genomics demonstrating the value and performance of Dovetail's proximity ligation-based next-generation sequencing (NGS) library prep solutions on Element's AVITI System. (biospace.com)
- Their proximity ligation technology couples well with Element's improvements in sequencing accuracy, lower optical duplication rate and consistency of data, across a range of applications," said Shawn Levy, Senior Vice President of Applications and Scientific Affairs at Element Biosciences. (biospace.com)
- We are thrilled to partner with Element Biosciences to add Dovetail Genomics' cutting-edge proximity ligation technology to this new and exciting NGS platform," said Dovetail CEO Todd Dickinson. (biospace.com)
- Through Dovetail Genomics' proprietary proximity ligation technologies, the 3D architecture of the genome is captured alongside primary sequence information using standard next-generation sequencing (NGS) approaches. (biospace.com)
- Internal iliac (hypogastric) artery ligation was pioneered by Howard Kelly for the treatment of intraoperative bleeding from cervical cancer prior to its application in postpartum hemorrhage (PPH). (medscape.com)
- A study by Boynukalin et al suggested that bilateral hypogastric artery ligation may be an effective therapeutic option for severe PPH and is worth considering in the setting of an obstetric emergency. (medscape.com)
- In the transabdominal or transperitoneal approach to internal iliac (hypogastric) artery ligation, the patient is adequately prepared "from nipples to knees" with 1% iodine in 70% alcohol or 0.5% chlorhexidine in 70% alcohol. (medscape.com)
- Ligation of the internal iliac (hypogastric) artery can be performed either via an open approach (intraperitoneal or extraperitoneal) or via an endovascular approach. (medscape.com)
- Did Experimenter Bias Conceal the Efficacy of Spinal Opioids in Previous Studies with the Spinal Nerve Ligation Model of Neuropathic Pain? (asahq.org)
- Therefore, we focus this discussion on whether spinal delivery of opioids has analgesic effects, regardless of the duration of action, in the commonly used spinal nerve ligation model of neuropathic pain. (asahq.org)
- A laboratory model of neuropathic pain in rats in which ligation of low lumbar spinal nerves results in reduced withdrawal threshold to tactile stimulation of the paw, akin to mechanical allodynia in patients with neuropathic pain, was first described over a decade ago. (asahq.org)
- Neuropathic pain is now considered to be only infrequently sympathetic-dependent and often responds to spinal opioids, and these changes in understanding of clinical neuropathic pain have been mirrored by changes in results obtained in this spinal nerve ligation model. (asahq.org)
- Although other methods can stem hemorrhage in patients with pelvic bleeding due to trauma or PPH, it is essential for surgeons to be aware of the indications for and technique of internal iliac artery ligation. (medscape.com)
- Background and evidence of complete mesocolic excision with central vascular ligation. (symposium.it)
- Single-stranded DNA (ssDNA) ligation is a crucial step in many biochemical assays. (nih.gov)
- 10 Several laboratories, including ours, showed that the spinal nerve ligation model was also resistant to treatment with intrathecal morphine. (asahq.org)
- Keep reading to know about that and other risks of pregnancy after tubal ligation. (momjunction.com)
- Major complications are also rare, occurring in less than 2% of tubal ligations. (verywellhealth.com)
- A study by Schellenberg et al (N = 77) assessed complications after temporary bilateral internal iliac artery ligation for control of pelvic hemorrhage in the setting of trauma. (medscape.com)
- This ligation strategy involves the merger of a peptide salicylaldehyde (SAL) ester and a peptide with N-terminal serine or threonine, to afford an N,O -benzylidene acetal at the conjugation site, followed by simple acidolysis to release the natural peptidic bond. (frontiersin.org)
- 3. BERGER, J. B. Self-ligation in the year 2000. (bvsalud.org)
- Do annealed double-stranded oligos need to be modified prior to ligation into the appropriate restriction site of my cloning vector? (idtdna.com)
- There are several different tubal ligation procedures. (healthnews.com)
- Having a tubal ligation is one of the most common procedures done by women to prevent pregnancy. (hotzehwc.com)
- In the view of the authors, these results suggested that a high degree of concern for gluteal necrosis after temporary bilateral internal iliac artery ligation in severely injured trauma patients is unfounded and should not prevent the use of this technique in such patients. (medscape.com)
- Tubal ligations are a safe and effective way to prevent pregnancy. (healthnews.com)
- We sought to further investigate the correlation of improvement in semen parameters and pregnancy rates after varicocele ligation (VL), for the purpose of better defining significant improvement. (auanet.org)
- Here Are The Pros And Cons Of Ligation? (smartparenting.com.ph)
- Tubal ligation is a permanent contraceptive technique for women. (momjunction.com)
Vector and insert1
- This tutorial describes the use of the NEBioCalculator web tool to optimize the molar ratio between vector and insert DNA for use in a ligation reaction. (neb.com)
- This approach identifies toxHPUF-encoding genes through comparison of gene-specific read coverages in DNA from pooled ligation mixtures before electroporation and pooled transformants after electroporation. (helsinki.fi)
- The market estimations and size provided in the report are based on a comprehensive analysis of recent & futuristic key developments in the ligation devices market. (researchdive.com)
- The Global Ligation Devices Market Research Report presents a short evaluate including the aggressive panorama and key developments, policies, production costs, and processes. (articlemania.xyz)