Application of a ligature to tie a vessel or strangulate a part.
Poly(deoxyribonucleotide):poly(deoxyribonucleotide)ligases. Enzymes that catalyze the joining of preformed deoxyribonucleotides in phosphodiester linkage during genetic processes during repair of a single-stranded break in duplex DNA. The class includes both EC 6.5.1.1 (ATP) and EC 6.5.1.2 (NAD).
Procedures that render the female sterile by interrupting the flow in the FALLOPIAN TUBE. These procedures generally are surgical, and may also use chemicals or physical means.
Swollen veins in the lower part of the RECTUM or ANUS. Hemorrhoids can be inside the anus (internal), under the skin around the anus (external), or protruding from inside to outside of the anus. People with hemorrhoids may or may not exhibit symptoms which include bleeding, itching, and pain.
An enzyme that catalyzes the conversion of linear RNA to a circular form by the transfer of the 5'-phosphate to the 3'-hydroxyl terminus. It also catalyzes the covalent joining of two polyribonucleotides in phosphodiester linkage. EC 6.5.1.3.
The 31 paired peripheral nerves formed by the union of the dorsal and ventral spinal roots from each spinal cord segment. The spinal nerve plexuses and the spinal roots are also included.
The channels that collect and transport the bile secretion from the BILE CANALICULI, the smallest branch of the BILIARY TRACT in the LIVER, through the bile ductules, the bile ducts out the liver, and to the GALLBLADDER for storage.
The blind sac or outpouching area of the LARGE INTESTINE that is below the entrance of the SMALL INTESTINE. It has a worm-like extension, the vermiform APPENDIX.
Systemic inflammatory response syndrome with a proven or suspected infectious etiology. When sepsis is associated with organ dysfunction distant from the site of infection, it is called severe sepsis. When sepsis is accompanied by HYPOTENSION despite adequate fluid infusion, it is called SEPTIC SHOCK.
Naturally occurring or experimentally induced animal diseases with pathological processes sufficiently similar to those of human diseases. They are used as study models for human diseases.
Incision of tissues for injection of medication or for other diagnostic or therapeutic procedures. Punctures of the skin, for example may be used for diagnostic drainage; of blood vessels for diagnostic imaging procedures.
Catalyze the joining of preformed ribonucleotides or deoxyribonucleotides in phosphodiester linkage during genetic processes. EC 6.5.1.
A member of the tumor necrosis factor receptor superfamily with specificity for CD40 LIGAND. It is found on mature B-LYMPHOCYTES and some EPITHELIAL CELLS, lymphoid DENDRITIC CELLS. Evidence suggests that CD40-dependent activation of B-cells is important for generation of memory B-cells within the germinal centers. Mutations of the gene for CD40 antigen result in HYPER-IGM IMMUNODEFICIENCY SYNDROME, TYPE 3. Signaling of the receptor occurs through its association with TNF RECEPTOR-ASSOCIATED FACTORS.
Impairment of bile flow due to obstruction in small bile ducts (INTRAHEPATIC CHOLESTASIS) or obstruction in large bile ducts (EXTRAHEPATIC CHOLESTASIS).
A strain of albino rat used widely for experimental purposes because of its calmness and ease of handling. It was developed by the Sprague-Dawley Animal Company.
The intracellular transfer of information (biological activation/inhibition) through a signal pathway. In each signal transduction system, an activation/inhibition signal from a biologically active molecule (hormone, neurotransmitter) is mediated via the coupling of a receptor/enzyme to a second messenger system or to an ion channel. Signal transduction plays an important role in activating cellular functions, cell differentiation, and cell proliferation. Examples of signal transduction systems are the GAMMA-AMINOBUTYRIC ACID-postsynaptic receptor-calcium ion channel system, the receptor-mediated T-cell activation pathway, and the receptor-mediated activation of phospholipases. Those coupled to membrane depolarization or intracellular release of calcium include the receptor-mediated activation of cytotoxic functions in granulocytes and the synaptic potentiation of protein kinase activation. Some signal transduction pathways may be part of larger signal transduction pathways; for example, protein kinase activation is part of the platelet activation signal pathway.
A molecule that binds to another molecule, used especially to refer to a small molecule that binds specifically to a larger molecule, e.g., an antigen binding to an antibody, a hormone or neurotransmitter binding to a receptor, or a substrate or allosteric effector binding to an enzyme. Ligands are also molecules that donate or accept a pair of electrons to form a coordinate covalent bond with the central metal atom of a coordination complex. (From Dorland, 27th ed)
The internal fragments of precursor proteins (INternal proTEINS) that are autocatalytically removed by PROTEIN SPLICING. The flanking fragments (EXTEINS) are ligated forming mature proteins. The nucleic acid sequences coding for inteins are considered to be MOBILE GENETIC ELEMENTS. Inteins are composed of self-splicing domains and an endonuclease domain which plays a role in the spread of the intein's genomic sequence. Mini-inteins are composed of the self-splicing domains only.
The largest bile duct. It is formed by the junction of the CYSTIC DUCT and the COMMON HEPATIC DUCT.
Dilated blood vessels in the ESOPHAGUS or GASTRIC FUNDUS that shunt blood from the portal circulation (PORTAL SYSTEM) to the systemic venous circulation. Often they are observed in individuals with portal hypertension (HYPERTENSION, PORTAL).
Elements of limited time intervals, contributing to particular results or situations.
NECROSIS of the MYOCARDIUM caused by an obstruction of the blood supply to the heart (CORONARY CIRCULATION).
Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others.
A congenital heart defect characterized by the persistent opening of fetal DUCTUS ARTERIOSUS that connects the PULMONARY ARTERY to the descending aorta (AORTA, DESCENDING) allowing unoxygenated blood to bypass the lung and flow to the PLACENTA. Normally, the ductus is closed shortly after birth.
Intense or aching pain that occurs along the course or distribution of a peripheral or cranial nerve.
IMMUNOGLOBULINS on the surface of B-LYMPHOCYTES. Their MESSENGER RNA contains an EXON with a membrane spanning sequence, producing immunoglobulins in the form of type I transmembrane proteins as opposed to secreted immunoglobulins (ANTIBODIES) which do not contain the membrane spanning segment.
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
Differentiation antigens residing on mammalian leukocytes. CD stands for cluster of differentiation, which refers to groups of monoclonal antibodies that show similar reactivity with certain subpopulations of antigens of a particular lineage or differentiation stage. The subpopulations of antigens are also known by the same CD designation.
The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.
One of the mechanisms by which CELL DEATH occurs (compare with NECROSIS and AUTOPHAGOCYTOSIS). Apoptosis is the mechanism responsible for the physiological deletion of cells and appears to be intrinsically programmed. It is characterized by distinctive morphologic changes in the nucleus and cytoplasm, chromatin cleavage at regularly spaced sites, and the endonucleolytic cleavage of genomic DNA; (DNA FRAGMENTATION); at internucleosomal sites. This mode of cell death serves as a balance to mitosis in regulating the size of animal tissues and in mediating pathologic processes associated with tumor growth.
Impairment of bile flow in the large BILE DUCTS by mechanical obstruction or stricture due to benign or malignant processes.
A strain of albino rat developed at the Wistar Institute that has spread widely at other institutions. This has markedly diluted the original strain.
Enlarged and tortuous VEINS.
A tumor necrosis factor receptor subtype found in a variety of tissues and on activated LYMPHOCYTES. It has specificity for FAS LIGAND and plays a role in regulation of peripheral immune responses and APOPTOSIS. Multiple isoforms of the protein exist due to multiple ALTERNATIVE SPLICING. The activated receptor signals via a conserved death domain that associates with specific TNF RECEPTOR-ASSOCIATED FACTORS in the CYTOPLASM.
A membrane glycoprotein and differentiation antigen expressed on the surface of T-cells that binds to CD40 ANTIGENS on B-LYMPHOCYTES and induces their proliferation. Mutation of the gene for CD40 ligand is a cause of HYPER-IGM IMMUNODEFICIENCY SYNDROME, TYPE 1.
Strains of mice in which certain GENES of their GENOMES have been disrupted, or "knocked-out". To produce knockouts, using RECOMBINANT DNA technology, the normal DNA sequence of the gene being studied is altered to prevent synthesis of a normal gene product. Cloned cells in which this DNA alteration is successful are then injected into mouse EMBRYOS to produce chimeric mice. The chimeric mice are then bred to yield a strain in which all the cells of the mouse contain the disrupted gene. Knockout mice are used as EXPERIMENTAL ANIMAL MODELS for diseases (DISEASE MODELS, ANIMAL) and to clarify the functions of the genes.
The geometric and structural changes that the HEART VENTRICLES undergo, usually following MYOCARDIAL INFARCTION. It comprises expansion of the infarct and dilatation of the healthy ventricle segments. While most prevalent in the left ventricle, it can also occur in the right ventricle.
Bleeding in any segment of the GASTROINTESTINAL TRACT from ESOPHAGUS to RECTUM.
A hypoperfusion of the BLOOD through an organ or tissue caused by a PATHOLOGIC CONSTRICTION or obstruction of its BLOOD VESSELS, or an absence of BLOOD CIRCULATION.
A deoxyribonucleotide polymer that is the primary genetic material of all cells. Eukaryotic and prokaryotic organisms normally contain DNA in a double-stranded state, yet several important biological processes transiently involve single-stranded regions. DNA, which consists of a polysugar-phosphate backbone possessing projections of purines (adenine and guanine) and pyrimidines (thymine and cytosine), forms a double helix that is held together by hydrogen bonds between these purines and pyrimidines (adenine to thymine and guanine to cytosine).
Morphologic alteration of small B LYMPHOCYTES or T LYMPHOCYTES in culture into large blast-like cells able to synthesize DNA and RNA and to divide mitotically. It is induced by INTERLEUKINS; MITOGENS such as PHYTOHEMAGGLUTININS, and by specific ANTIGENS. It may also occur in vivo as in GRAFT REJECTION.
The vein accompanying the femoral artery in the same sheath; it is a continuation of the popliteal vein and becomes the external iliac vein.
The excision of in-frame internal protein sequences (INTEINS) of a precursor protein, coupled with ligation of the flanking sequences (EXTEINS). Protein splicing is an autocatalytic reaction and results in the production of two proteins from a single primary translation product: the intein and the mature protein.
Polymers made up of a few (2-20) nucleotides. In molecular genetics, they refer to a short sequence synthesized to match a region where a mutation is known to occur, and then used as a probe (OLIGONUCLEOTIDE PROBES). (Dorland, 28th ed)
Glycoproteins found on the membrane or surface of cells.
The muscle tissue of the HEART. It is composed of striated, involuntary muscle cells (MYOCYTES, CARDIAC) connected to form the contractile pump to generate blood flow.
The largest lymphatic vessel that passes through the chest and drains into the SUBCLAVIAN VEIN.
Treatment of varicose veins, hemorrhoids, gastric and esophageal varices, and peptic ulcer hemorrhage by injection or infusion of chemical agents which cause localized thrombosis and eventual fibrosis and obliteration of the vessels.
The presence of chyle in the thoracic cavity. (Dorland, 27th ed)
A group of compounds with an 8-carbon ring. They may be saturated or unsaturated.
Lymphocytes responsible for cell-mediated immunity. Two types have been identified - cytotoxic (T-LYMPHOCYTES, CYTOTOXIC) and helper T-lymphocytes (T-LYMPHOCYTES, HELPER-INDUCER). They are formed when lymphocytes circulate through the THYMUS GLAND and differentiate to thymocytes. When exposed to an antigen, they divide rapidly and produce large numbers of new T cells sensitized to that antigen.
Antibodies produced by a single clone of cells.
Maintenance of blood flow to an organ despite obstruction of a principal vessel. Blood flow is maintained through small vessels.
The process in which substances, either endogenous or exogenous, bind to proteins, peptides, enzymes, protein precursors, or allied compounds. Specific protein-binding measures are often used as assays in diagnostic assessments.
Lymphoid cells concerned with humoral immunity. They are short-lived cells resembling bursa-derived lymphocytes of birds in their production of immunoglobulin upon appropriate stimulation.
RNA sequences that serve as templates for protein synthesis. Bacterial mRNAs are generally primary transcripts in that they do not require post-transcriptional processing. Eukaryotic mRNA is synthesized in the nucleus and must be exported to the cytoplasm for translation. Most eukaryotic mRNAs have a sequence of polyadenylic acid at the 3' end, referred to as the poly(A) tail. The function of this tail is not known for certain, but it may play a role in the export of mature mRNA from the nucleus as well as in helping stabilize some mRNA molecules by retarding their degradation in the cytoplasm.
Disease or damage involving the SCIATIC NERVE, which divides into the PERONEAL NERVE and TIBIAL NERVE (see also PERONEAL NEUROPATHIES and TIBIAL NEUROPATHY). Clinical manifestations may include SCIATICA or pain localized to the hip, PARESIS or PARALYSIS of posterior thigh muscles and muscles innervated by the peroneal and tibial nerves, and sensory loss involving the lateral and posterior thigh, posterior and lateral leg, and sole of the foot. The sciatic nerve may be affected by trauma; ISCHEMIA; COLLAGEN DISEASES; and other conditions. (From Adams et al., Principles of Neurology, 6th ed, p1363)
Molecules on the surface of T-lymphocytes that recognize and combine with antigens. The receptors are non-covalently associated with a complex of several polypeptides collectively called CD3 antigens (ANTIGENS, CD3). Recognition of foreign antigen and the major histocompatibility complex is accomplished by a single heterodimeric antigen-receptor structure, composed of either alpha-beta (RECEPTORS, ANTIGEN, T-CELL, ALPHA-BETA) or gamma-delta (RECEPTORS, ANTIGEN, T-CELL, GAMMA-DELTA) chains.
A CELL LINE derived from human T-CELL LEUKEMIA and used to determine the mechanism of differential susceptibility to anti-cancer drugs and radiation.
The introduction of a phosphoryl group into a compound through the formation of an ester bond between the compound and a phosphorus moiety.
Experimentally induced chronic injuries to the parenchymal cells in the liver to achieve a model for LIVER CIRRHOSIS.
Either of two extremities of four-footed non-primate land animals. It usually consists of a FEMUR; TIBIA; and FIBULA; tarsals; METATARSALS; and TOES. (From Storer et al., General Zoology, 6th ed, p73)
The rate dynamics in chemical or physical systems.
Costimulatory T-LYMPHOCYTE receptors that have specificity for CD80 ANTIGEN and CD86 ANTIGEN. Activation of this receptor results in increased T-cell proliferation, cytokine production and promotion of T-cell survival.
A short thick vein formed by union of the superior mesenteric vein and the splenic vein.
Non-antibody proteins secreted by inflammatory leukocytes and some non-leukocytic cells, that act as intercellular mediators. They differ from classical hormones in that they are produced by a number of tissue or cell types rather than by specialized glands. They generally act locally in a paracrine or autocrine rather than endocrine manner.
The movement and the forces involved in the movement of the blood through the CARDIOVASCULAR SYSTEM.
Established cell cultures that have the potential to propagate indefinitely.
A branch of the celiac artery that distributes to the stomach, pancreas, duodenum, liver, gallbladder, and greater omentum.
The spatial arrangement of the atoms of a nucleic acid or polynucleotide that results in its characteristic 3-dimensional shape.
The veins and arteries of the HEART.
An increased sensation of pain or discomfort produced by mimimally noxious stimuli due to damage to soft tissue containing NOCICEPTORS or injury to a peripheral nerve.
Conversion of an inactive form of an enzyme to one possessing metabolic activity. It includes 1, activation by ions (activators); 2, activation by cofactors (coenzymes); and 3, conversion of an enzyme precursor (proenzyme or zymogen) to an active enzyme.
The color-furnishing portion of hemoglobin. It is found free in tissues and as the prosthetic group in many hemeproteins.
RNA that has catalytic activity. The catalytic RNA sequence folds to form a complex surface that can function as an enzyme in reactions with itself and other molecules. It may function even in the absence of protein. There are numerous examples of RNA species that are acted upon by catalytic RNA, however the scope of this enzyme class is not limited to a particular type of substrate.
Ducts that collect PANCREATIC JUICE from the PANCREAS and supply it to the DUODENUM.
Either of the two principal arteries on both sides of the neck that supply blood to the head and neck; each divides into two branches, the internal carotid artery and the external carotid artery.
A large lobed glandular organ in the abdomen of vertebrates that is responsible for detoxification, metabolism, synthesis and storage of various substances.
A generic term for all substances having the properties of stretching under tension, high tensile strength, retracting rapidly, and recovering their original dimensions fully. They are generally POLYMERS.
Procedures of applying ENDOSCOPES for disease diagnosis and treatment. Endoscopy involves passing an optical instrument through a small incision in the skin i.e., percutaneous; or through a natural orifice and along natural body pathways such as the digestive tract; and/or through an incision in the wall of a tubular structure or organ, i.e. transluminal, to examine or perform surgery on the interior parts of the body.
Organic chemistry methodology that mimics the modular nature of various biosynthetic processes. It uses highly reliable and selective reactions designed to "click" i.e., rapidly join small modular units together in high yield, without offensive byproducts. In combination with COMBINATORIAL CHEMISTRY TECHNIQUES, it is used for the synthesis of new compounds and combinatorial libraries.
The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.
A positive regulatory effect on physiological processes at the molecular, cellular, or systemic level. At the molecular level, the major regulatory sites include membrane receptors, genes (GENE EXPRESSION REGULATION), mRNAs (RNA, MESSENGER), and proteins.
The domestic dog, Canis familiaris, comprising about 400 breeds, of the carnivore family CANIDAE. They are worldwide in distribution and live in association with people. (Walker's Mammals of the World, 5th ed, p1065)
The main artery of the thigh, a continuation of the external iliac artery.
A non-essential amino acid. In animals it is synthesized from PHENYLALANINE. It is also the precursor of EPINEPHRINE; THYROID HORMONES; and melanin.
Surgical removal of the ductus deferens, or a portion of it. It is done in association with prostatectomy, or to induce infertility. (Dorland, 28th ed)
The vein which drains the foot and leg.
An enzyme that catalyzes the transfer of a phosphate group to the 5'-terminal hydroxyl groups of DNA and RNA. EC 2.7.1.78.
A nerve which originates in the lumbar and sacral spinal cord (L4 to S3) and supplies motor and sensory innervation to the lower extremity. The sciatic nerve, which is the main continuation of the sacral plexus, is the largest nerve in the body. It has two major branches, the TIBIAL NERVE and the PERONEAL NERVE.
The vessels carrying blood away from the capillary beds.
Complex of at least five membrane-bound polypeptides in mature T-lymphocytes that are non-covalently associated with one another and with the T-cell receptor (RECEPTORS, ANTIGEN, T-CELL). The CD3 complex includes the gamma, delta, epsilon, zeta, and eta chains (subunits). When antigen binds to the T-cell receptor, the CD3 complex transduces the activating signals to the cytoplasm of the T-cell. The CD3 gamma and delta chains (subunits) are separate from and not related to the gamma/delta chains of the T-cell receptor (RECEPTORS, ANTIGEN, T-CELL, GAMMA-DELTA).
The development of new BLOOD VESSELS during the restoration of BLOOD CIRCULATION during the healing process.
The parts of a macromolecule that directly participate in its specific combination with another molecule.
Serum glycoprotein produced by activated MACROPHAGES and other mammalian MONONUCLEAR LEUKOCYTES. It has necrotizing activity against tumor cell lines and increases ability to reject tumor transplants. Also known as TNF-alpha, it is only 30% homologous to TNF-beta (LYMPHOTOXIN), but they share TNF RECEPTORS.
Operative procedures for the treatment of vascular disorders.
Any of the processes by which nuclear, cytoplasmic, or intercellular factors influence the differential control (induction or repression) of gene action at the level of transcription or translation.
Laboratory mice that have been produced from a genetically manipulated EGG or EMBRYO, MAMMALIAN.
The largest branch of the celiac trunk with distribution to the spleen, pancreas, stomach and greater omentum.
The facilitation of a chemical reaction by material (catalyst) that is not consumed by the reaction.
The flow of BLOOD through or around an organ or region of the body.
INFLAMMATION of the PERITONEUM lining the ABDOMINAL CAVITY as the result of infectious, autoimmune, or chemical processes. Primary peritonitis is due to infection of the PERITONEAL CAVITY via hematogenous or lymphatic spread and without intra-abdominal source. Secondary peritonitis arises from the ABDOMINAL CAVITY itself through RUPTURE or ABSCESS of intra-abdominal organs.
A group of deoxyribonucleotides (up to 12) in which the phosphate residues of each deoxyribonucleotide act as bridges in forming diester linkages between the deoxyribose moieties.
A condition characterized by the dilated tortuous veins of the SPERMATIC CORD with a marked left-sided predominance. Adverse effect on male fertility occurs when varicocele leads to an increased scrotal (and testicular) temperature and reduced testicular volume.
Antigens expressed primarily on the membranes of living cells during sequential stages of maturation and differentiation. As immunologic markers they have high organ and tissue specificity and are useful as probes in studies of normal cell development as well as neoplastic transformation.
A polynucleotide consisting essentially of chains with a repeating backbone of phosphate and ribose units to which nitrogenous bases are attached. RNA is unique among biological macromolecules in that it can encode genetic information, serve as an abundant structural component of cells, and also possesses catalytic activity. (Rieger et al., Glossary of Genetics: Classical and Molecular, 5th ed)
Evaluation undertaken to assess the results or consequences of management and procedures used in combating disease in order to determine the efficacy, effectiveness, safety, and practicability of these interventions in individual cases or series.
A ubiquitously expressed membrane glycoprotein. It interacts with a variety of INTEGRINS and mediates responses to EXTRACELLULAR MATRIX PROTEINS.
Injuries to the PERIPHERAL NERVES.

Site of myocardial infarction. A determinant of the cardiovascular changes induced in the cat by coronary occlusion. (1/2428)

The influence of site of acute myocardial infarction on heart rate, blood pressure, cardiac output, total peripheral resistance (TPR), cardiac rhythm, and mortality was determined in 58 anesthetized cats by occlusion of either the left anterior descending (LAD), left circumflex or right coronary artery. LAD occlusion resulted in immediate decrease in cardiac output, heart rate, and blood pressure, an increase in TPR, and cardiac rhythm changes including premature ventricular beats, ventricular tachycardia, and occasionally ventricular fibrillation. The decrease in cardiac output and increase in TPR persisted in the cats surviving a ventricular arrhythmia. In contrast, right coronary occlusion resulted in a considerably smaller decrease in cardiac output. TPR did not increase, atrioventricular condition disturbances were common, and sinus bradycardia and hypotension persisted in the cats recovering from an arrhythmia. Left circumflex ligation resulted in cardiovascular changes intermediate between those produced by occlusion of the LAD or the right coronary artery. Mortality was similar in each of the three groups. We studied the coronary artery anatomy in 12 cats. In 10, the blood supply to the sinus node was from the right coronary artery and in 2, from the left circumflex coronary artery. The atrioventricular node artery arose from the right in 9 cats, and from the left circumflex in 3. The right coronary artery was dominant in 9 cats and the left in 3. In conclusion, the site of experimental coronary occlusion in cats is a major determinant of the hemodynamic and cardiac rhythm changes occurring after acute myocardial infarction. The cardiovascular responses evoked by ligation are related in part to the anatomical distribution of the occluded artery.  (+info)

Control of ketogenesis from amino acids. IV. Tissue specificity in oxidation of leucine, tyrosine, and lysine. (2/2428)

In vitro and in vivo studies were made on the tissue specificity of oxidation of the ketogenic amino acids, leucine, tyrosine, and lysine. In in vitro studies the abilities of slices of various tissues of rats to form 14CO2 from 14C-amino acids were examined. With liver, but not kidney slices, addition of alpha-ketoglutarate was required for the maximum activities with these amino acids. Among the various tissues tested, kidney had the highest activity for lysine oxidation, followed by liver; other tissues showed very low activity. Kidney also had the highest activity for leucine oxidation, followed by diaphragm; liver and adipose tissue had lower activities. Liver had the highest activity for tyrosine oxidation, but kidney also showed considerable activity; other tissues had negligible activity. In in vivo studies the blood flow through the liver or kidney was stopped by ligation of the blood vessels. Then labeled amino acids were injected and recovery of radioactivity in respiratory 14CO2 was measured. In contrast to results with slices, no difference was found in the respiratory 14CO2 when the renal blood vessels were or were not ligated. On the contrary ligation of the hepatic vessels suppressed the oxidations of lysine and tyrosine completely and that of leucine partially. Thus in vivo, lysine and tyrosine seem to be metabolized mainly in the liver, whereas leucine is metabolized mostly in extrahepatic tissues and partly in liver. Use of tissue slices seems to be of only limited value in elucidating the metabolisms of these amino acids.  (+info)

Pulsed Doppler ultrasonographic evaluation of portal blood flow in dogs with experimental portal vein branch ligation. (3/2428)

Portal blood flow was measured using pulsed Doppler ultrasound in 6 dogs before and after left portal vein branch ligation. Mean portal vein blood flow velocity and mean portal vein blood flow were significantly reduced after ligation and the congestion index was increased (p < 0.01). Pulsed Doppler ultrasound studies provide valuable physiological information which may assist the clinician with the diagnosis of canine hepatic circulatory disorders.  (+info)

Effects of chronic nitric oxide activation or inhibition on early hepatic fibrosis in rats with bile duct ligation. (4/2428)

Hepatic fibrosis or increased liver collagen contents drive functional abnormalities that, when extensive, may be life threatening. The purpose of this study was to assess the effects of the chronic stimulation or inhibition of nitric oxide synthesis in rats with hepatic fibrosis induced by permanent common bile duct ligation (3 weeks) and the role of expression of the different nitric oxide synthase isoforms. Bile duct ligation led to an important accumulation of collagen in the hepatic parenchyma, as shown both histologically and by the hydroxyproline contents of livers. Bilirubin and serum enzyme activities (measured as markers of cholestasis) increased several-fold after bile duct ligation. The area of fibrotic tissue, liver hydroxyproline content and serum markers of cholestasis were clearly related in obstructed rats. The absence of modifications in haemodynamic parameters excludes circulatory changes from being responsible for the development of liver alterations. In animals treated with NG-nitro-L-arginine methyl ester (L-NAME) the area of fibrosis was similar to that of untreated animals, the signs of cholestasis and cellular injury being more evident. In rats treated with L-arginine the area of fibrosis was almost three times larger than that found in bile duct ligated rats and in L-NAME-treated bile duct ligated rats, although the observed biochemical changes were similar to those seen in rats treated with L-NAME. Our results with inducible nitric oxide synthase, obtained by Western blots and immunohistochemistry, indicate a greater expression of the inducible enzyme in bile duct ligated and L-arginine-treated animals and a lower expression in the L-NAME and control groups. Constitutive nitric oxide synthase expression, obtained by Western blots, was very similar in all groups, except for the L-arginine-treated rats in which it was lower. These results suggest that nitric oxide production may be a key factor in the development of fibrosis in bile duct ligated rats. They also support the hypothesis of a dual role for nitric oxide; one beneficial, mediated by its circulatory effects, and the second negative, through its local toxic effects.  (+info)

Uninjured C-fiber nociceptors develop spontaneous activity and alpha-adrenergic sensitivity following L6 spinal nerve ligation in monkey. (5/2428)

We investigated whether uninjured cutaneous C-fiber nociceptors in primates develop abnormal responses after partial denervation of the skin. Partial denervation was induced by tightly ligating spinal nerve L6 that innervates the dorsum of the foot. Using an in vitro skin-nerve preparation, we recorded from uninjured single afferent nerve fibers in the superficial peroneal nerve. Recordings were made from 32 C-fiber nociceptors 2-3 wk after ligation and from 29 C-fiber nociceptors in control animals. Phenylephrine, a selective alpha1-adrenergic agonist, and UK14304 (UK), a selective alpha2-adrenergic agonist, were applied to the receptive field for 5 min in increasing concentrations from 0.1 to 100 microM. Nociceptors from in vitro control experiments were not significantly different from nociceptors recorded by us previously in in vivo experiments. In comparison to in vitro control animals, the afferents found in lesioned animals had 1) a significantly higher incidence of spontaneous activity, 2) a significantly higher incidence of response to phenylephrine, and 3) a higher incidence of response to UK. In lesioned animals, the peak response to phenylephrine was significantly greater than to UK, and the mechanical threshold of phenylephrine-sensitive afferents was significantly lower than for phenylephrine-insensitive afferents. Staining with protein gene product 9.5 revealed an approximately 55% reduction in the number of unmyelinated terminals in the epidermis of the lesioned limb compared with the contralateral limb. Thus uninjured cutaneous C-fiber nociceptors that innervate skin partially denervated by ligation of a spinal nerve acquire two abnormal properties: spontaneous activity and alpha-adrenergic sensitivity. These abnormalities in nociceptor function may contribute to neuropathic pain.  (+info)

Hypothermic neuroprotection of peripheral nerve of rats from ischaemia-reperfusion injury. (6/2428)

Although there is much information on experimental ischaemic neuropathy, there are only scant data on neuroprotection. We evaluated the effectiveness of hypothermia in protecting peripheral nerve from ischaemia-reperfusion injury using the model of experimental nerve ischaemia. Forty-eight male Sprague-Dawley rats were divided into six groups. We used a ligation-reperfusion model of nerve ischaemia where each of the supplying arteries to the sciatic-tibial nerves of the right hind limb was ligated and the ligatures were released after a predetermined period of ischaemia. The right hind limbs of one group (24 rats) were made ischaemic for 5 h and those of the other group (24 rats) for 3 h. Each group was further divided into three and the limbs were maintained at 37 degrees C (36 degrees C for 5 h of ischaemia) in one, 32 degrees C in the second and 28 degrees C in the third of these groups for the final 2 h of the ischaemic period and an additional 2 h of the reperfusion period. A behavioural score was recorded and nerve electrophysiology of motor and sensory nerves was undertaken 1 week after surgical procedures. At that time, entire sciatic-tibial nerves were harvested and fixed in situ. Four portions of each nerve were examined: proximal sciatic nerve, distal sciatic nerve, mid-tibial nerve and distal tibial nerve. To determine the degree of fibre degeneration, each section was studied by light microscopy, and we estimated an oedema index and a fibre degeneration index. The groups treated at 36-37 degrees C underwent marked fibre degeneration, associated with a reduction in action potential and impairment in behavioural score. The groups treated at 28 degrees C (for both 3 and 5 h) showed significantly less (P < 0.01; ANOVA, Bonferoni post hoc test) reperfusion injury for all indices (behavioural score, electrophysiology and neuropathology), and the groups treated at 32 degrees C had scores intermediate between the groups treated at 36-37 degrees C and 28 degrees C. Our results showed that cooling the limbs dramatically protects the peripheral nerve from ischaemia-reperfusion injury.  (+info)

Mid-term results of endoscopic perforator vein interruption for chronic venous insufficiency: lessons learned from the North American subfascial endoscopic perforator surgery registry. The North American Study Group. (7/2428)

PURPOSE: The safety, feasibility, and early efficacy of subfascial endoscopic perforator surgery (SEPS) for the treatment of chronic venous insufficiency were established in a preliminary report. The long-term clinical outcome and the late complications after SEPS are as yet undetermined. METHODS: The North American Subfascial Endoscopic Perforator Surgery registry collected information on 148 SEPS procedures that were performed in 17 centers in the United States and Canada between August 1, 1993, and February 15, 1996. The data analysis in this study focused on mid-term outcome in 146 patients. RESULTS: One hundred forty-six patients (79 men and 67 women; mean age, 56 years; range, 27 to 87 years) underwent SEPS. One hundred and one patients (69%) had active ulcers (class 6), and 21 (14%) had healed ulcers (class 5). One hundred and three patients (71%) underwent concomitant venous procedures (stripping, 70; high ligation, 17; varicosity avulsion alone, 16). There were no deaths or pulmonary embolisms. One deep venous thrombosis occurred at 2 months. The follow-up periods averaged 24 months (range, 1 to 53 months). Cumulative ulcer healing at 1 year was 88% (median time to healing, 54 days). Concomitant ablation of superficial reflux and lack of deep venous obstruction predicted ulcer healing (P <.05). Clinical score improved from 8.93 to 3.98 at the last follow-up (P <. 0001). Cumulative ulcer recurrence at 1 year was 16% and at 2 years was 28% (standard error, < 10%). Post-thrombotic limbs had a higher 2-year cumulative recurrence rate (46%) than did those limbs with primary valvular incompetence (20%; P <.05). Twenty-eight of the 122 patients (23%) who had class 5 or class 6 ulcers before surgery had an active ulcer at the last follow-up examination. CONCLUSIONS: The interruption of perforators with ablation of superficial reflux is effective in decreasing the symptoms of chronic venous insufficiency and rapidly healing ulcers. Recurrence or new ulcer development, however, is still significant, particularly in post-thrombotic limbs. The reevaluation of the indications for SEPS is warranted because operations in patients without previous deep vein thrombosis are successful but operations in those patients with deep vein thrombosis are less successful. Operations on patients with deep vein occlusion have poor outcomes.  (+info)

Contribution of extracranial lymphatics and arachnoid villi to the clearance of a CSF tracer in the rat. (8/2428)

The objective of this study was to determine the relative roles of arachnoid villi and cervical lymphatics in the clearance of a cerebrospinal fluid (CSF) tracer in rats. 125I-labeled human serum albumin (125I-HSA; 100 micrograms) was injected into one lateral ventricle, and an Evans blue dye-rat protein complex was injected intravenously. Arterial blood was sampled for 3 h. Immediately after this, multiple cervical vessels were ligated in the same animals, and plasma recoveries were monitored for a further 3 h after the intracerebroventricular injection of 100 micrograms 131I-HSA. Tracer recovery in plasma at 3 h averaged (%injected dose) 0.697 +/- 0.042 before lymphatic ligation and dropped significantly to 0.357 +/- 0. 060 after ligation. Estimates of the rate constant associated with the transport of the CSF tracer to plasma were also significantly lower after obstruction of cervical lymphatics (from 0.584 +/- 0. 072/h to 0.217 +/- 0.056/h). No significant changes were observed in sham-operated animals. Assuming that the movement of the CSF tracer to plasma in lymph-ligated animals was a result of arachnoid villi clearance, we conclude that arachnoid villi and extracranial lymphatic pathways contributed equally to the clearance of the CSF tracer from the cranial vault.  (+info)

Results:. Rebleeding tended to be less frequent with ligation than with sclerotherapy: 10 of 38 (26%) compared with 17 of 39 (44%) (difference, 17% [95% CI, 4% to 38%]), but results in the two groups were comparable for blood transfusions, for length of hospital stay, and for risk of death. Comparison of Kaplan-Meier estimates of time to rebleeding and death showed no statistical differences between treatments. Complications were less common in the ligation group: fewer patients in the ligation group had esophageal strictures (0 of 38 compared with 13 of 39 [33%]; P , 0.001) and had complicated esophageal ulcers (1 of 38 [2.6%] compared with 6 of 39 [15%]; P = 0.11). In addition, fewer ligation treatments were required to achieve variceal eradication (4.1 0.3 compared with 6.2 0.4; P , 0.001). ...
Results: Thirty seven files were included in the analysis. Files were divided in two groups according to the surgical technique performed; group (A) included 20 patients received transduodenal suture ligation of the bleeder and periduodenal triple ligation of the gastroduodenal, right gastro-epiploic and superior pancreatico-duodenal arteries and group (B) included 17 patients received only transduodenal suture ligation of the bleeder. There was neither rebleeding nor mortality in group (A). In group (B), four patients developed rebleeding postoperatively. Bleeding was haemodynamically insignificant and stopped spontaneously in 1 patient, 1 patient exsanguinated and died before re-surgery and 2 patients had re-surgery; one survived after periduodenal arterial ligation and the other died postoperatively due to complications of gastrectomy ...
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Most commonly, following ligation the circularised plasmid, now containing your insert DNA, is transformed into competent bacteria for further selection and analysis. In bacteria, transformation refers to a genetic change brought about by taking up and expressing DNA i.e. your plasmid construct, and competence refers to the state of being able to take up DNA; most bacteria are not naturally transformable, but are made permeable to the plasmid DNA by chemical or electrochemical means. Competent cells are extremely fragile and should be handled gently, specifically kept cold and not vortexed. The transformation procedure is efficient enough for most lab purposes, with efficiencies as high as 109 transformed cells per microgram of DNA, but it is important to realize that even with high efficiency cells only 1 DNA molecule in about 10,000 is successfully transformed ...
Experimental design. Litters of CD-1 pups were purchased from Charles River Laboratories, Inc. Pups with dams (litter size = 10) were delivered on P3 and allowed to acclimate. Food and water were provided ad libitum. Doses for LM used in vivo and in vitro were determined from previously described in vitro pharmacokinetics (14). Pups of both sexes at P7 and P10 (see Supplemental Table 1 for sample sizes) were i.p. administered (Figure 1A) 0.25 mg/kg LM dissolved in isotonic PBS as 2 treatment groups: (a) 1 dose 2 hours before ligation (pre-LM) and (b) immediately following ligation (post-LM). After 1 hour of baseline EEG recording, pups were given a loading dose of PB (25 mg/kg, i.p.). Doses for HIOC used in vivo and in vitro were determined from previously described in vitro pharmacokinetics (16). Pups of both sexes at P7 were i.p. administered the full agonist selective for TrkB 5 mg/kg HIOC dissolved in 95%/5% isotonic PBS/DMSO as 2 treatment groups: (a) 1 dose 2 hours before ligation ...
Congenital adrenal dsd) hyperplasia (cah), numerically the most important of regular hepatic function and comblete blood count (cbc) drawn. The long-term results have yet to be a competition between grafts of human umbilical cord- derived mesenchymal stem cells embedded in the treat- 9 months of starting ezetimibe treatment. The patient is over the course of sibling screening papillae no longer the rst 52 hours right subcostal pain, is usually taken after the injection site to reduce the incidence of testicular malignancies in the differentiation capabilities of self- renewal, grow indefinitely, and differentiate into a more robust following ligation of individual differences in order to prevent problems with medication such as job loss, college graduation, or the fluoroscopy unit in a model or in a. Rosenbaum d, mattina a, koch e, rossant f, gallo a, kachenoura n. Effects of disease processes with their microenvironment. Flat mood and attitudes. The patient has an adequate potassium level, ...
from Brad Barbazuk Goal is to generate PCR fragments that contain the ends of PAC inserts that can be sequenced. For inverse PCR we cut the PAC once in the vector (near outward primer site) and once at unknown site in insert (and many other places outside these sites, that dont matter). Following ligation to generate small circles, PCR is performed with outward facing primers at the end of the vector that will amplify a product that contains the end of the insert. To increase the odds of getting an amplifiable product, we use two different enzymes (used singly!!) per pac end - NlaIII and RsaI for the SP6 end, and NlaIII and HinPI (or HhaI) for the T7 end. Primers ...
Objective-Accumulating evidence suggests a role of semaphorins in vascular homeostasis. Here, we investigate the role of Sema7A (semaphorin 7A) in atherosclerosis and its underlying mechanism. Approach and Results-Using genetically engineered Sema7A -/- ApoE -/- mice, we showed that deletion of Sema7A attenuates atherosclerotic plaque formation primarily in the aorta of ApoE -/- mice on a high-fat diet. A higher level of Sema7A in the atheroprone lesser curvature suggests a correlation of Sema7A with disturbed flow. This notion is supported by elevated Sema7A expression in human umbilical venous endothelial cells either subjected to oscillatory shear stress or treated with the PKA (protein kinase A)/CREB (cAMP response element-binding protein) inhibitor H89 (N-[2-(p-bromocinnamylamino)ethyl]-5-isoquinolinesulfonamide·2HCl hydrate). Further studies using the partial carotid artery ligation model showed that disturbed flow in the left carotid artery of Sema7A +/+ ApoE -/- mice promoted the ...
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If its simple ligation and therefore expect positives you may need the plasmid so better make minipreps. You can check by restriction analysis. Find enzymes that will give different restriction pattern if you have t he insert or not. Anyway, you may need to sequence your plasmid at the end, depending what you need it for, to check correct ligation sites, etc. ...
Day 3 1) The oocytes were transferred back into a 1.5 ml Eppendorf tube. 2) Excess unbound primary antibodies were removed by washing 3 times for 5 min at RT on a rocker with 1ml of 1 x Wash Buffer A (provided by the kit). 3) The oocytes were transferred again into parafilm stuffed PCR tube and then incubated with 20 ul PLA probe Minus, 20 ul PLA probe Plus and 60 ul of antibody diluent (all provided by the kit) for 2 hours and 30 min at 37 deg C with gentle rocking. 4) The oocytes were then transferred back into a 1.5 ml Eppendorf tube. 5) Excess unbound secondary antibodies were removed by washing 3 times for 5 min at RT on a rocker with 1ml of 1 x Wash Buffer A (provided by the kit). 6) The oocytes were then incubated with the ligation solution (20 ul of 5 x ligation stock, 77.5 ul of Sigma H2O (SH2O) and 2.5 ul Ligase) provided by the kit for 1 hour at 37 deg C with gentle rocking. 7) The oocytes were transferred back into a 1.5 ml Eppendorf tube. 8) Excess unbound ligation solution was ...
Dear netters- Ive repeatedly tried to do some cloning using an AflII site...and Ive tried a couple of obvious tricks to get any useful efficiency, but to no avail. Specifically, I have a 650 bp. EcoRI/Afl II fragment Im trying to get into the same sites of pSL1180 (superlinker from pharmacia). Production of the fragments goes well, and I even have tried CIP-ing the vector (just in case...)...Ive tried ligation +/- PEG, high and low amounts of DNA, at 12 degrees C and room temp., and high and low ligase (400 and 2000 NEB units). And all I get are (very few) weirdo clones; that is, clones i cant explain from fer instance, insertion of a dimer of my insert. (and these represent 3x more colonies than without insert!) Any ideas? Thanks in advance!! Micky Krol Inst. for Molec. Virology UW-Madison em-ay-kay-are-oh-ell at students.wisc.edu (translate the first part into letters to use this address ...
Protocol After completion of the End Repair/dA-Tailing program add 1.0 μl of adaptor (or vector if generating an expression library)
Protocol After completion of the End Repair/dA-Tailing step add 1.0 μl of adaptor (or vector if generating an expression library)
SC 18:03, 30 August 2012 (EDT): Removed 1mg/ml BSA component from protocol, per the fact that it is not recommended in the NEB protocol ...
Please, i need your opignon about what ive done. Is it correct to dont inactivate enzyme (phosphatase) after a diphosphatasing of a plasmid and do the next step which is ligation, without inactivation ? is this could affect my results ...
Test of selectivity among closely related RNAs. Shown are initial rates of Q-STAR signaling after incubation under ligation/RCA conditions for the times indicat
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TY - JOUR. T1 - Hypotension following patent ductus arteriosus ligation. T2 - The role of adrenal hormones. AU - Clyman, Ronald I.. AU - Wickremasinghe, Andrea. AU - Merritt, T. Allen. AU - Solomon, Tabitha. AU - McNamara, Patrick. AU - Jain, Amish. AU - Singh, Jaideep. AU - Chu, Alison. AU - Noori, Shahab. AU - Sekar, Krishnamurthy. AU - Lavoie, Pascal M.. AU - Attridge, Joshua T.. AU - Swanson, Jonathan R.. AU - Gillam-Krakauer, Maria. AU - Reese, Jeff. AU - Demauro, Sara. AU - Poindexter, Brenda. AU - Aucott, Sue. AU - Satpute, Monique. AU - Fernandez, Erika. AU - Auchus, Richard J.. PY - 2014/6. Y1 - 2014/6. N2 - Objective To test the hypothesis that an impaired adrenal response to stress might play a role in the hypotension that follows patent ductus arteriosus (PDA) ligation. Study design We performed a multicenter study of infants born at ,32 weeks gestation who were about to undergo PDA ligation. Serum adrenal steroids were measured 3 times: before and after a cosyntropin (1.0 μg/kg) ...
TY - JOUR. T1 - TRAIL mediates liver injury by the innate immune system in the bile duct-ligated mouse. AU - Kahraman, Alisan. AU - Barreyro, Fernando J.. AU - Bronk, Steven F.. AU - Werneburg, Nathan W.. AU - Mott, Justin L. AU - Akazawa, Yuko. AU - Masuoka, Howard C.. AU - Howe, Charles L.. AU - Gores, Gregory J.. PY - 2008/4/1. Y1 - 2008/4/1. N2 - The contribution of tumor necrosis factor-related apoptosis-inducing ligand (TRAIL), a death ligand expressed by cells of the innate immune system, to cholestatic liver injury has not been explored. Our aim was to ascertain if TRAIL contributes to liver injury in the bile duct-ligated (BDL) mouse. C57/BL6 wild-type (wt), TRAIL heterozygote (TRAIL+/-), and TRAIL knockout (TRAIL-/-) mice were used for these studies. Liver injury and fibrosis were examined 7 and 14 days after BDL, respectively. Hepatic TRAIL messenger RNA (mRNA) was 6-fold greater in BDL animals versus sham-operated wt animals (P , 0.01). The increased hepatic TRAIL expression was ...
TY - JOUR. T1 - The pathologic effect of ureteral ligation, with clinical implications. AU - Gurin, J. I.. AU - Garcia, R. L.. AU - Melman, A.. AU - Leiter, E.. PY - 1982/1/1. Y1 - 1982/1/1. N2 - Inadvertent, ureteral ligation is a frequent occurrence with pelvic surgery. We determined the effect of the ureteral ligation of varying lengths of time in an animal model in order to provide a rational basis for the clinical management of such injuries in humans. In the rat, simple deligation of a ureter that has been ligated for as long as 24 hours results in a complete resolution of the injury with no gross or microscopic evidence of the trauma. After 48 and 72 hours of ligation, hydroureter was apparent.. AB - Inadvertent, ureteral ligation is a frequent occurrence with pelvic surgery. We determined the effect of the ureteral ligation of varying lengths of time in an animal model in order to provide a rational basis for the clinical management of such injuries in humans. In the rat, simple ...
The impact of feeding after endoscopic treatment of gastroesophageal varices has never been investigated. It is still unknown whether early feeding may increase early rebleeding in patients with acute esophageal variceal bleeding treated with EVL. It is customary for clinicians to institute fasting for 2 or 3 days after emergency EVL. This may be a safe approach to watch against early rebleeding. However, many patients would be fasting for a longer time and nutrition may be impaired, possibly resulting in aggravation of ascites. Thus, the investigators conduct a controlled study to evaluate whether early feeding have a bad impact on patients receiving emergency EVL or histoacryl injection for bleeding gastric varices ...
The data supplied here convey a correlation of PDA closure with ventricular size/OFC normalization (Figs. 2-4) and represent the first documentation of this temporal relationship in the literature. The results must be interpreted with caution and should not be viewed as proof of a link between hsPDA, cerebral hemodynamics, and ventricular outflow. Although hydrocephalus is a well-documented consequence of PIVH,3,23 our case suggests that the unstable hemodynamic environment due to left-to-right shunting may also impede CSF outflow and, in addition to an underlying degree of IVH, may contribute mechanistically to ventriculomegaly. This relationship may not have been previously identified due to the nationwide variation in PDA diagnosis and treatment strategy,5 or to the lack of a definition surrounding PDA hemodynamic significance.6. It has been previously shown that a systemic hemodynamic change can immediately follow hsPDA ligation. Bissonnette and Benson detected both acute decline in CVP and ...
The present invention provides for methods and compositions for gas separation and purification utilizing a metallo-organic polymer adsorbent in processes for separating carbon dioxide, water, nitrogen oxides and hydrocarbons from gas streams. The metallo-organic polymer adsorbent composition has the formula: [R(L) n ] m M n , wherein R represents an organic spacer selected from the group consisting of an organic cyclic or acyclic compound; L represents a ligation group substituted on the organic spacer selected from the group consisting of carboxylate group, -C(═O)O − ; dithiocarboxylate group, -C(═S)S − ; and β-diketonate group, -C(═O)C(R′)═C(-O − )-, wherein R′═H, or an aliphatic or aromatic group; M represents a transition metal or a rare earth metal selected from the group consisting of I to VIIB and VIII metals; m is the oxidation state of transition metal; and n is the number of the ligation group substituted on the organic
Safety, feasibility, and efficacy of associating liver partition and portal vein ligation for staged hepatectomy in treating hepatocellular carcinoma: a systematic review
TY - JOUR. T1 - Therapeutic face-off. T2 - Band ligation versus beta blockage for variceal binding. AU - Green, J. A.. AU - Amaro, R.. AU - Barkin, J. S.. PY - 2000/5/25. Y1 - 2000/5/25. N2 - Sarin et al. prospectively compared propranolol treatment and endoscopic ligation for primary prevention of esophageal variceal bleeding. The patients were randomized to receive either beta-blocker therapy with an aim to decrease heart rate by 25% or weekly variceal ligation until obliteration was achieved. The study population of 89 patients were at high risk for bleeding as determined by the presence of large varices, defined as ,5 mm in diameter. Eighty-two of these patients were cirrhotic. Forty-four were treated with propranolol, and 45 underwent variceal ligation. The mean duration of post- treatment follow-up for the medical arm was 14 months compared to 13 months for the endoscopic group. The mean time required to achieve the desired heart rate reduction was approximately 2.5 days, and an average of ...
Anybody can get hemorrhoids especially now that diets have been leaning towards foods that have low fiber material and a high content of processed ingredients. Lack of proper water intake and the design of current toilet bowls also make the chances of getting hemorrhoids bigger. The cause of hemorrhoids is an increased pressure within the hemorrhoidal veins but there are countless number of ways that this increased pressure occurs. These are the risk factors that general increase the chance of one possessing a hemorrhoid ...
Learn more about Endoscopic Band Ligation at Memorial Hospital DefinitionReasons for ProcedurePossible ComplicationsWhat to ExpectCall Your Doctorrevision ...
Learn more about Endoscopic Band Ligation at Trident Health System DefinitionReasons for ProcedurePossible ComplicationsWhat to ExpectCall Your Doctorrevision ...
1. The inhibiting action of urine on catalase depends to a great extent on its reaction. The urine of a nephritic has no greater inhibiting power than normal urine provided the reaction of urines tested is neutral. 2. The catalytic activity of a single rabbits blood is constant from day to day. 3. There is considerable variation in the catalytic action of the blood of different rabbits. 4. Following ligation of the ureters the catalytic activity of the blood gradually decreases; the tissues of an animal which has died as a result of ligation of the ureters show a decided decrease in catalytic activity, when compared with those of normal animals. 5. Following bilateral nephrectomy the same decline of the catalytic action of the blood is observed as after bilateral ligation of ureters. 6. Following unilateral nephrectomy the catalytic activity of the blood may temporarily fall and then rise above its previous level, or it may fall continuously. Wherever the continuous fall occurs the animal dies. ...
congenital fistulae are often difficult to treat because of multiple fistulae and the tendency for more fistulae to open up following ligation of the more obvious ones. Conservative treatment with compression stockings may be the best ...
A slidable kit for endoscopic ligation of gastric and esophageal varices comprises an endoscope-inserting cylinder and a ligating device coaxially and integrally secured to the endoscope-inserting cylinder, where the ligating device is in such a structure that a slide cylinder having an annular rib on the outer periphery at the rearward end is inserted into an outer cylinder having an annular rib on the inner periphery at the forward end; an inner cylinder is inserted into the slide cylinder; the outer cylinder and the inner cylinder are integrated together at the rearward ends and firmly secured to the forward end of the endoscope-inserting cylinder; an elastic O ring is mounted on the outer periphery at the forward end of the inner cylinder; a seal ring is provided at the rearward end of the slide cylinder, thereby keeping an annular space between the outer cylinder, the inner cylinder and the slide cylinder hermetic; the slide cylinder is slidable forwards by a liquid introduced into the annular
Myocardial infarction (MI) is a disease of major consequence in the modern world, causing permanent, irreversible damage to the heart. Survivors are at risk for developing further cardiovascular pathologies such as heart failure. Further study of MI injury is crucial to improve the understanding and treatment of the post-MI heart. The most commonly used model for MI in vivo is surgical ligation of the left anterior descending coronary artery (LAD). There are two predominant approaches: permanent ligation (PL), where the LAD is permanently occluded with a suture, or ischaemia-reperfusion (IR), where the LAD is temporarily occluded before removing the suture to restore blood flow and tissue reperfusion. PL results in the majority of the area at risk becoming infarcted, leading to significant apoptotic cell death and a large scar. Conversely, IR salvages some of the area at risk; thus, the scar is smaller and includes reperfusion injury, an additional, albeit smaller, second wave of necrotic damage. PL may
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Some patients develop IBS as a result of poor nutritional choices while others get IBS because of stress If you have irritable bowel syndrome, you should meet with a specialist to determine what is causing this condition. The alternation of extreme temperature causes the hemorrhoids to piles spotting during early pregnancy and disappear. Many Homeopathic ingredients are effective in reducing the inflammation and pain associated with hemorrhoids. Rubber band ligation is frequently recommended for individuals with first- or second-degree hemorrhoids and, in some circumstances, third-degree hemorrhoids.
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For the Rapid DNA Ligation Kit, we guarantee , 1 x 106 transformants per μg religated pUC18 vector (sticky end as well as blunt end), i.e., this means that only 2.96 pg of vector (1 molecule of pUC 18 = of 2,96 ag [attogram!]), have be to be religated to result in this yield in transformants. This rate of religation is easily achieved in 5 minutes with the Rapid DNA Ligation Kit.. Note: This also means that when ligation products are checked using an agarose gel, a substantial proportion of the vector DNA used will still show up „unligated“. Considering the above mentioned molecular weight of pUC 18 this is is easily visualized.. The rate and kinetics of concatemer ligation in cloning with λ-vectors are totally different from plasmid ligation. For ligation of concatemers ligase has to connect linear fragments in the presence of high amounts of DNA. For plasmid cloning an insert must first be ligated to one end of the linearized plasmid-vector, and then the other end of the same ...
For this procedure, you will lie on your left side. If you are not intubated a mouthpiece will be placed to help keep your mouth open. An assistant will be in the room to monitor your breathing and heartbeat. You may also be given oxygen through your nose. A suction tube will be used to clear the saliva and other fluids from your mouth.. A lubricated endoscope will be placed into your mouth. It will be passed down your throat and into your esophagus. The scope will have a small light and a camera. The doctor will watch the images on a video monitor. Air will be passed through the scope to help your doctor see your esophagus. The doctor will be able to locate the enlarged vein.. Instruments will be passed through the scope. The enlarged tissue will be sucked into the devices chamber. One or more bands will be placed around the tissue to clamp off the blood supply. ...
An endoscopic instrument for the application of ligature binders and a ligature are described. A base body and an actuation part of a handle part are longitudinally displaceable relative to each other against an elastic force within a fixed range. Connected to the actuation part is an actuation rod at whose distal end a first ratchet heat is secured. A shaft, firmly connected to the base body, carries a second ratchet head at its distal end. With the help of the ratchet heads, which are matched to the toothed end zone of a ligature binder, a ligature can be drawn together. A ligature whose end zone has already been inserted into the endoscopic instrument prior to the operation can be brought into a closed shape with the help of a constricted zone in its binding part and of a longitudinal slot in its headpiece.
Sigma-Aldrich offers abstracts and full-text articles by [Bin Tian, Xue-Long Wang, Ya Huang, Li-Hua Chen, Ruo-Xiao Cheng, Feng-Ming Zhou, Ran Guo, Jun-Cheng Li, Tong Liu].
DNA Ligation Kit, Mighty Mix, is a one-solution premix ligation reagent that offers high efficiency ligation in as little as 5 minutes. Small reaction volumes and efficient for blunt end and TA cloning.
Bevezetés: A májtumorok reszekálhatóságának egyetlen biológiai feltétele a májreszekció után megmaradó, a májműködést még biztosítani képes elégséges májszövetmennyiség (FLR). A műtét után visszamaradó májszövet tömege még a műtét előtt megnöveszthető vena portae okklúziós eljárásokkal. A legújabb ilyen eljárás az Associating Liver Partition and Portal vein ligation for Staged hepatectomy (ALPPS), mely a legrövidebb idő alatt a legjelentősebb indukált hipertrófiát biztosítja. A műtét morbiditása és mortalitása az eljárás bevezetése idején azonban elfogadhatatlanul magas volt. Célkitűzés: A szövődmények csökkentése a betegszelekció pontosításával és a műtéti technika módosításával. Módszer: Műtéttechnikai módosításként a teljes mobilizálás technika helyett klinikánkon ún. no touch technikát dolgoztunk ki. Klinikánk betegadatait nemzetközi regiszterbe kapcsoltuk, melyben gyűjtjük a demográfiai ...
Kovács, Tibor and Billes, Viktor and Komlós, Marcell and Hotzi, Bernadette and Manzéger, Anna and Tarnóci, Anna and Papp, Diána and Szikszai, Fanni and Szinyákovics, Janka and Ákos Rácz, Ákos and Noszál, Béla and Veszelka, Szilvia and Walter, Fruzsina R. and Deli, Mária A. and Hackler Jr., László and Alföldi, Róbert and Huzian, Orsolya and Puskás, László G. and Liliom, Hanna and Tárnok, Krisztián and Schlett, Katalin and Borsy, Adrienn and Welker, Ervin and Kovács, Attila L. and Pádár, Zsolt and Erdős, Attila and Légrádi, Ádám and Bjelik, Annamária and Gulya, Károly and Gulyás, Balázs and Vellai, Tibor (2017) The small molecule AUTEN-99 (autophagy enhancer-99) prevents the progression of neurodegenerative symptoms. SCIENTIFIC REPORTS. pp. 1-17. ISSN 2045-2322 Kovács, Tibor and Máthé, Domokos and Fülöp, András and Jemnitz, Katalin and Bátai-Konczos, Attila (2017) Functional shift with maintained regenerative potential following portal vein ligation. ...
In the past I have had two main problems with failed ligation reactions. The first appeared to involve a seasonal decrease in the quality of MilliQ water (associated with filters which were not changed often enough). This wouldnt appear to have affected you, as your phosphorylated vector was religated. The second problem I have encountered, involves over CIPing your vector. Cheaper preparations of phosphatase have traces of exonuclease activity, these will nibble the ends of DNA. The more common problem is the carry over of CIP into the ligation reaction. This effectively removes the phosphates from your insert, thus no ligation and no transformants. I routinely use no more than 1unit CIP/pmole DNA ends (routinely 0.2unit/pmoleDNA). I heat inactivate the CIP (65C/20mins) in the presence of 5mM EDTA, phenol extract and gel purify the linearised vector. This method maybe a bit over the top, but I have had no problems cloning when my vector is purified in this way. Alternatively, one can use ...
The Rovner P-1RL Platinum Alto Saxophone Ligature is a piece of metal ligature that includes a Rovner Cap. Producing a pure, dark, tone this ligature creates a ringing bell-like sound and enables effortless playing with an evenness of scale, smoothing out the difficult transitions between registers. Providing you with superior control for a secure, centered tone and flawless intonation, the Platinum Ligature allows for unprecedented reed-gripping capabilities.
Some women complain of serious medical problems after having their tubes tied. They even have a name for it: post-tubal ligation syndrome. But doctors have yet to find anything wrong.
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This article, describes conditions for shortening ligation time when cloning into the pGEM-T Vector Systems. A 15-minute ligation gave approximately 50% transformants by blue/white selection with further improvements when BSA was added.
Doctors give trusted, helpful answers on causes, diagnosis, symptoms, treatment, and more: Dr. Schomberg-Klaiss on can you become pregnant after a tubular ligation: There is a low failure rate for tubal ligation - so it is potentially possible.
Start Over You searched for: Collections Images from the History of Medicine (IHM) ✖Remove constraint Collections: Images from the History of Medicine (IHM) Languages English ✖Remove constraint Languages: English Subjects Ligation ✖Remove constraint Subjects: Ligation Subjects Patients ✖Remove constraint Subjects: Patients Genre Photographs ✖Remove constraint Genre: Photographs Dates by Range 1900-1949 ✖Remove constraint Dates by Range: 1900-1949 Dates by Range 1950-1999 ✖Remove constraint Dates by Range: 1950-1999 ...
You know the best explanation to that guys is that....whatever plans that we are going to make and this plan is not Gods plan, then, it will not really happen.No matter how many times your tubes are being tied, if its Gods will that you will get pregnant nobody and nothing can stop Gods plan even tubal ligation.. ...
Your success starts with experimental design, and each decision has an impact. We offer a comprehensive supply of best-in-class reagents to fit every cloning project-choose the kit thats right for you:. ...
If you have a question about this talk, please contact Sarah Adderley.. Abstract not available. This talk is part of the BSS Internal Seminars series.. ...
"Tubal Ligation". Johns Hopkins Medicine. "Vasectomy". Johns Hopkins Medicine. Lin, Luo; Wu Shi-Zhong; Zhu Changmin; Fan Qifu; ... There are multiple ways of having sterilization done, but the two that are used most frequently are tubal ligation for women ... A 1996 Chinese study found that "risk for depression was 2.34 times greater after tubal ligation, and 3.97 times greater after ... Surgical sterilization methods include: Tubal ligation in females, known popularly as "having one's tubes tied". The fallopian ...
One such method employs the technique of partial ligation. DNA ligation is the process by which linear DNA pieces are connected ... Bowen, R (20 October 1999). "DNA Ligation". Biotechnology and Genetic Engineering. Retrieved 12 November 2013. CS1 maint: ...
1959 Aug;66:640-8. Tubal ligation. Green GH. N Z Med J. 1958 Oct;57(321):470-7. Foetal renal hypoplasia and the origin of ...
... the tetrazine ligation, the isocyanide-based click reaction, and most recently, the quadricyclane ligation. The use of ... The Staudinger ligation has been used in both live cells and live mice. The azide can act as a soft electrophile that prefers ... The Staudinger Ligation The reaction was modified to include an ester group ortho to the phosphorus atom on one of the aryl ... The Staudinger ligation is a reaction developed by the Bertozzi group in 2000 that is based on the classic Staudinger reaction ...
Glal hydrolysis and Ligation Adapter Dependent PCR assay (GLAD-PCR assay) is the novel method to determine R(5mC)GY sites ... The universal adapter ligation. As an adapter an oligonucleotide duplex 5'-CCTGCTCTTTCATCG-3'/3'-pGGACGAGAAAGTAGCp-5' is used, ...
ISBN 978-4-431-55823-1. Nuijens T, Schmidt M (2019). Enzyme-mediated ligation methods. Humana, New York, NY. ISBN 978-1-4939- ...
Their suturing ligation without excision]". Khirurgiia (in Bulgarian). 43 (4): 65-8. PMID 2097429. Serdev N (1991). "[Free ...
"Multiplex Ligation-Dependent Probe Amplification". The Journal of Molecular Diagnostics. 8 (4): 433-443. doi:10.2353/jmoldx. ... multiplex ligation-dependent probe amplification (MLPA), array comparative genomic hybridization (aCGH), and EHMT1 sequencing. ...
Endonucleolytic cleavage and ligation "Ptc1". Saccharomyces Genome Database. SGD Project. Retrieved 21 March 2014. CS1 maint: ...
Additional ligation of the intersphincteric fistula tract did not improve the outcome after endorectal advancement flap. ... ligation of intersphincteric fistula tract) procedure. LIFT procedure is based on secure closure of the internal opening and ... the ligation of intersphincteric fistula tract". J Med Assoc Thai. 90 (3): 581-6. PMID 17427539. van Onkelen, RS; Gosselink, MP ... ligation of intersphincteric tract close to the internal opening and removal of intersphincteric tract, scraping out all ...
He, Ye; Hinklin, Ronald; Chang, Jiyoung; Kiessling, Laura (2004). "Stereoselective N-Glycosylation by Staudinger Ligation". ...
Hypogastric artery ligation. Bilateral ligation of the internal iliac arteries reduces the pulse pressure of blood flowing to ... with or without ligation of the tubo-ovarian vessels. Ligation of the uterine and utero-ovarian arteries can decrease uterine ... Shahin AY, Farghaly TA, Mohamed SA, Shokry M, Abd-El-Aal DE, Youssef MA (March 2010). "Bilateral uterine artery ligation plus B ... Clark SL, Phelan JP, Yeh S, Bruce SR, Paul RH (1985). "Hypogastric Artery Ligation for Obstetric Hemorrhage". Obstetrics & ...
Surgeons thought that ligation of the intersphincteric tract close to the internal opening might solve the problem. Surgeons ... However, Rojanasakul reported the ligation of intersphincteric fistula tract in 2007 with apparent satisfactory results ... the ligation of intersphincteric fistula tract. J Med Assoc Thai. 2007 Mar;90(3):581-6.] [Matos D, Lunniss PJ, Phillips RKS ( ... ligation of intersphincteric fistula tract) procedure. LIFT procedure is based on secure closure of the internal opening and ...
Weil Briefing: Patent Ligation and Licensing. (June 3, 2010). Siegal, Matthew W. and Hansen, Kristopher M. "Free and Clear" ...
... and ligation, performed by ubiquitin ligases (E3). The result of this process is the formation of a covalent bond between the C ...
Ligation products are then digested by DpnII. This enzyme cuts non-methylated GATCs, ensuring that only fragments flanked by ...
"Cleavage-dependent ligation by the FLP recombinase. Characterization of a mutant FLP protein with an alteration in a catalytic ...
The invention of native chemical ligation methods by Phil Dawson and Stephen Kent in the mid-1990s opened up prospects for ... Dawson PE, Muir TW, Clark-Lewis I, Kent SB (1994). "Synthesis of proteins by native chemical ligation". Science. 266 (5186): ...
Tubal ligation is considered a permanent procedure.[citation needed] While a full testing of tubal functions in patients with ... Midsegment tubal obstruction can be due to tubal ligation procedures as that part of the tube is a common target of ...
Other ligands such as PPh3 and TBIA can also be used, even though PPh3 is liable to Staudinger ligation with the azide ... Rieder, Ulrike; Luedtke, Nathan W. (25 August 2014). "Alkene-tetrazine ligation for imaging cellular DNA". Angew Chem Int Ed ... Existing reactions, such as Staudinger ligation and the Huisgen 1,3-dipolar cycloaddition, have been modified and optimized for ... Ramil, Carlo P; Lin, Qing (August 2014). "Photoclick chemistry: a fluorogenic light-triggered in vivo ligation reaction". ...
Tubbs, Oswald S. (1 July 1944). "The effect of ligation on infection of the patent ductus arteriosus". British Journal of ... ISSN 1365-2168.(subscription required) Gross, Robert; Hubbard, John (1939). "Surgical Ligation of a Patent Ductus Arteriosus". ...
Tubal ligation can predispose to ectopic pregnancy. Reversal of tubal sterilization (tubal reversal) carries a risk for ectopic ... This is higher if more destructive methods of tubal ligation (tubal cautery, partial removal of the tubes) have been used than ... and tubal ligation. A previous induced abortion does not appear to increase the risk. The intrauterine device (IUD) does not ...
A different chemistry can be exploited for protein ligation: the discovery of an intramolecular ester bond formation in ... "Harnessing ester bond chemistry for protein ligation". Chemical Communications. 53 (9): 1502-1505. doi:10.1039/C6CC09899A. PMID ...
Gross, Robert; Hubbard, John (1939). "Surgical Ligation of a Patent Ductus Arteriosus". Journal of the American Medical ...
The hammerhead ribozyme is an RNA motif that catalyzes reversible cleavage and ligation reactions at a specific site within an ... Canny MD, Jucker FM, Pardi A (2007). "Efficient ligation of the Schistosoma hammerhead ribozyme". Biochemistry. 46 (12): 3826- ... and may thus act as a substrate for hammerhead RNA-mediated ligation without a requirement for ATP or a similar exogenous ...
... detract nothing from the quality of the clip ligation, and add only seconds to case lengths. Both laparoscopy tubal ligations ... Clarke HC (April 1972). "Laparoscopy--new instruments for suturing and ligation". Fertility and Sterility. 23 (4): 274-7. doi: ... simple procedures such as a diagnostic laparoscopy or tubal ligation. The introduction in 1990 of a laparoscopic clip applier ...
https://doi.org/10.1136/bmj.1.4603.527-a. Pickerill, H P. (1949). "Ligation of the Mandibular (Inferior Dental) Artery". The ... Pickerill, H P. (1949). "Ligation of the Mandibular (Inferior Dental) Artery". British Medical Journal 1 (4603): 527. ...
New Zealand, in contrast, has higher levels of vasectomy than tubal ligation. 18% of all men, and 25% of all married men, have ... Levine, LA; Abern, MR; Lux, MM (2006). "Persistent motile sperm after ligation band vasectomy". The Journal of Urology. 176 (5 ... better than tubal ligations for which the failure rate is one in every 200 to 300 cases. A 2005 review including both early and ... In nearly every way that vasectomy can be compared to tubal ligation it has a more positive outlook. Vasectomy is more cost ...
This technique is favoured over band ligation because the position of the varices in the stomach, most often in the gastric ... Oesophageal varices have been treated by band ligation since the late 1980s. Non variceal indications include bleeding peptic ... Stiegmann GV, Goff JS, Sun JH, Wilborn S (February 1989). "Endoscopic elastic band ligation for active variceal hemorrhage". Am ... Rammohan A, Ravichandran P, Jeswanth S (2013). "Endoscopic band ligation for peptic ulcer bleed". OGH Reports. 2 (1): 15-18. ...
Synthesis of native proteins by chemical ligation. Dawson PE, Kent SB. Annu Rev Biochem. 2000;69:923-60. Moroder, Luis; ... Chicago and while professor at the Scripps Research Institute in the early 1990s was the inventor of native chemical ligation ...
See Tubal ligation methods below.) If the patient chooses a postpartum tubal ligation, the procedure will further depend on the ... Since most forms of tubal ligation require abdominal surgery under regional or general anesthesia, tubal ligation is also ... "postabortion tubal ligation", or more than six weeks after the end of a pregnancy, termed an "interval tubal ligation".[5] The ... See Tubal ligation methods below.) Regret[edit]. The majority of patients who undergo female sterilization procedures do not ...
Interval tubal ligation is not done after a recent delivery., in contrast to postpartum tubal ligation. ... In addition, a bilateral salpingectomy is effective as a tubal ligation procedure. A tubal ligation can be performed as a ... Tubal ligation or tubectomy (informally known as getting ones "tubes tied") is a form of female sterilization, in which the ... There are mainly four occlusion methods for tubal ligation, typically carried out on the isthmic portion of the fallopian tube ...
Simple ligation has a high failure rate, however, and modern procedures usually rely on stitching the tubes closed or severing ... tubal ligation, remains one of the most widely used. As originally performed, this consisted of tying the females fallopian ... Other articles where Tubal ligation is discussed: sterilization: …oldest form of surgical sterilization, ... Simple ligation has a high failure rate, however, and modern procedures usually rely on stitching the tubes closed or severing ...
... with effective ligation of those veins contributing to the varicocele. ...
Tubal ligation (getting your tubes tied) is surgery to prevent a woman from getting pregnant. Learn more about this permanent ... Tubal ligation - discharge (Medical Encyclopedia) Also in Spanish * Tubal ligation reversal (Medical Encyclopedia) Also in ... Tubal ligation (getting your "tubes tied") is a type of surgery. It prevents a woman from getting pregnant. It is a permanent ... Tubal Ligation (Mayo Foundation for Medical Education and Research) Also in Spanish ...
Tubal ligation is surgery to close a womans fallopian tubes. (It is sometimes called tying the tubes.) The fallopian tubes ... Tubal ligation can also be done right after you have a baby through a small cut in the navel. It can also be done during a C- ... Tubal ligation may be recommended for adult women who are sure they do not want to get pregnant in the future. The benefits of ... Tubal ligation is considered a permanent form of birth control. It is NOT recommended as a short-term method or one that can be ...
Tubal ligation is never morally permissible for the direct intention of preventing pregnancy. This is a moral principle which ... Automatically this third one should be by c section accompanied By a bilateral tubal ligation as future pregnancies may be ...
Tubal ligation is a surgical procedure that is used as a means to achieve (mostly) permanent infertility in women. As a means ... Tubal ligation is a surgical procedure that is used as a means to achieve (mostly) permanent infertility in women. As a means ... Reversible Tubal Ligation. This technique, once touted as a reversible procedure, uses either a spring clip or a silastic ( ... A tubal ligation, whether in veterinary or human medicine, only affects the oviducts. These small structures are isolated ...
Tubal ligation is a permanent type of female sterilization. Know the risks and what to expect. ... Tubal ligation. Tubal ligation. In a tubal ligation, the fallopian tubes are cut or blocked to disrupt the path normally taken ... Tubal ligation is one of the most commonly used surgical sterilization procedures for women. Tubal ligation permanently ... You may be allowed to go home several hours after an interval tubal ligation. Having a tubal ligation immediately following ...
... Cornelius Krasel krasel at wpxx02.toxi.uni-wuerzburg.de Mon Jan 19 04:27:18 EST 1998 *Previous message: ...
... newsnet customer customer at newsnet.com Sat May 6 09:19:09 EST 2006 *Previous message: Ligation failed ... Here is the ligation reaction: , , , , insert (12ng/ul) 2ul , , linearised vector (6ng/ul) 4ul , , 10X lig. buffer 1ul , , T4 ... Total DNA in ligation reaction is 48ng. , , insert:vector ratio is 1:1 in accordance to Roche T4 ligase information , , sheet ... Both the insert and the vector are large , enough that the intramolecular ligation has a higher chance than the , ...
Chapters cover topics such as omniligase-catalyzed inter- and intramolecular ligation; and ligation catalyzed by microbial ... This volume discusses different enzyme-catalyzed ligation methodologies for a variety of different chemical transformations. ... and intramolecular ligation; ligation catalyzed by microbial transglutaminase; peptide cyclization mediated by cyanobactin ... Cutting-edge and thorough, Enzyme-Mediated Ligation Methods is a valuable resource for students and scientists from different ...
... wanting to get pregnant after tubal ligation, stages of pregnancy from inception, how can i make my sim have a baby girl, ... The tubal ligation reversal requires that you go back into surgery to have your fallopian tubes rejoined. With a tubal ligation ... Wanting to get pregnant after tubal ligation. Getting pregnant after a tubal ligation is possible but requires additional ... Comments to «Wanting to get pregnant after tubal ligation». * dfdf. writes: 11.08.2014 at 10:23:19 Ache typically begins in the ...
They even have a name for it: post-tubal ligation syndrome. But doctors have yet to find anything wrong. ... Corson led a study that compared hormone levels in women who had had tubal ligation versus those of women who had not had the ... "Im not against tubal ligation," she says. "I just think women need to be told about the possible negative effects before they ... The exact number of women who, like Belcher, claim to have post-tubal ligation syndrome -- a range of symptoms including hot ...
hi my name is renee i had a tubal ligation done dec 2010 and it failed i am 9 months pregnant is it safe to get an essure even ... hi my name is renee i had a tubal ligation done dec 2010 and it failed i am 9 months pregnant is it safe to get an essure even ... It has also been noted that most pregnancies occurring after ligation are ectopic in nature, meaning that the pregnancy occurs ... Studies have shown that tubal ligation (tying of the fallopian tubes) is not a 100% guarantee of contraception. Theres still ...
Tubal ligation is a permanent form of birth control in which a womans fallopian tubes are tied or blocked. This keeps the egg ... Tubal Ligation. Facebook Twitter Linkedin Pinterest Print. What is tubal ligation? Tubal ligation is surgical procedure to ... What are the risks of a tubal ligation? Tubal ligation is safe, but all surgeries carry some risks. Serious problems occur in ... Home Health Treatment Tests and Therapies Tubal Ligation ... Ligation means to tie off. This prevents the egg and male sperm ...
The IPL reaction allows ligation of a synthetic peptide or a protein with an N-terminal cysteine residue to the C-terminus of ... Peptide Ligation. Product Listing Application Overview The IPL reaction allows the ligation of a synthetic peptide or a protein ... The transitory ligation product then undergoes a spontaneous S-N acyl rearrangement from a thioester to a stable peptide bond. ... Home Applications Protein Analysis & Tools Protein Labeling Peptide Ligation ...
Tubal Ligation Reversals have been performed for years, but the Essure procedure, which is stated to be non-reversible, has not ... The Essure procedure is a new type of Tubal Ligation, for women who want a permanent type of birth control or sterilization. ... The Essure procedure differs from other Tubal Ligation due to the fact that a coil-like device is inserted into the fallopian ... "Doctors Confirm First Successful Essure Tubal Ligation Reversal." Medical News Today. MediLexicon, Intl., 10 Oct. 2008. Web. ...
A tubal ligation reversal is a procedure to restore fertility after a woman has had a tubal ligation - a procedure that cuts or ... A tubal ligation reversal may allow a woman who had a tubal ligation to get pregnant without further medical assistance. There ... A tubal ligation reversal is more likely to be successful if there is still a large proportion of healthy tube. If your ... Tubal ligation reversal is abdominal surgery, which carries a risk of infection, bleeding and injury to nearby organs, as well ...
I had a tubal ligation 10 months ago and while I have heard of wo... ... I had a tubal ligation 10 months ago and while I have heard of women getting pregnant 10 yrs later, I didnt think it was ...
The ligation assembly may be separably secured to the ligation device. The trigger may be adapted to disengage the ligation ... A system may include a ligation device and/or an endoscopic tube or cannula for housing a ligation device. The ligation device ... A system, comprising: an endoscopic cannula for housing a ligation device; and a ligation device, including: a distal grasper ... The system of claim 17, further comprising a ligation assembly, wherein the ligation assembly is housed within the endoscopic ...
Tubal ligation with laparoscopic surgery. Tubal ligation can be accomplished with laparoscopic surgery. Youll have an IV line ... Tubal ligation is considered a permanent form of birth control. Youre not a good candidate if youre uncertain about having ... Tubal ligation is sometimes confused with hysterectomy, but theyre very different.. When you have a partial hysterectomy, your ... Because tubal ligation doesnt involve removal of your ovaries or uterus, in vitro fertilization is still an option. ...
Laparoscopic tubal ligation is a surgical sterilization procedure in which a womans fallopian tubes are either clamped and ... Tubal ligation is a permanent method of sterilization.. About 20% of women choose tubal ligation as their contraceptive method ... encoded search term (Laparoscopic Tubal Ligation) and Laparoscopic Tubal Ligation What to Read Next on Medscape ... Laparoscopic Tubal Ligation. Updated: Apr 03, 2018 * Author: Jessica L Versage, MD; Chief Editor: Christine Isaacs, MD more... ...
Home Applications Cloning & Synthetic Biology DNA Ligation Non-Cloning Ligation Non-Cloning Ligation. Product Listing ... Application Notes Non-Cloning Ligation *Efficient Adaptor Ligation for the Preparation of dsDNA Libraries using the Blunt/TA ... when were talking about ligation? In this webinar, NEB Scientist and ligase expert Greg Lohman discusses mismatch ligation by ... Many other detection methods capitalizing on the ligation of DNA are being employed by basic and applied research groups, as ...
This is the ligation part of the operation. The second step is where a thin flexible length of wire is inserted into the bottom ... Vein ligation and stripping This is the most common form of surgery for varicose veins. Vein stripping has been performed for a ... Recovery after vein ligation and stripping A surgical dressing or bandage is applied to the treated area. You will have to wear ... Advantages of vein ligation and stripping The main benefit from this surgery is that of removal of the offending vein. ...
Tubal ligation (sometimes called female sterilization) is a permanent form of birth control that is more than 99% effective at ... Previous Overview Next How safe is tubal ligation? Was this information helpful?. * Yes ... Remember, tubal ligation is super effective at preventing pregnancy, but it doesnt protect you or your partner from STDs. Use ... Tubal ligation is really great at preventing pregnancy - more than 99% effective. This means that fewer than 1 out of 100 women ...
ive ad a tubal ligation done 2 years ago wid the clips and over the past 3 weeks ive been getting really tired, sore breasts, ... ive ad a tubal ligation done 2 years ago wid the clips and over the past 3 weeks ive been getting really tired, sore breasts, ...
A tubal ligation is considered a permanent method of birth control. The fallopian tubes are cut or blocked, which prevents ... Laparoscopic tubal ligation leaves small scars. Laparoscopy is the preferred method of surgery for women who:. *Are in good ... For a laparoscopic tubal ligation, the surgeon makes two small cuts (incisions)-one in or just below the belly button (navel) ...
Due to the coronavirus outbreak worldwide, global demand for some personal protective equipment (PPE) is exceeding supply. In addition, manufacturing of PPE and many other wound care and infection prevention products have been impacted by global response to coronavirus. While you may see product availability reduction in the near-term, please be assured Medline continues to work to diversify production and provide your supply needs. As always, please reach out to your Medline sales representative with any specific questions or follow up. ...
A meta-analysis has shown that studies on the efficacy of PDA ligation in newborns neglect to adjust for confounders and ... Or ligation may improve survival, but infants who are referred for ligation may already be at a higher risk for neurologic ... Or ligation may lower mortality only because vulnerable medically treated infants die before they are referred for ligation and ... to surgical ligation. Ligation is usually considered after medical treatment has failed or when it is contraindicated. ...
  • The tubal ligation reversal requires that you go back into surgery to have your fallopian tubes rejoined. (amazonaws.com)
  • With a tubal ligation reversal, at best you have a 60% chance of it working well enough to naturally conceive. (amazonaws.com)
  • The chances are even less if there has been any damage to the fallopian tubes either as a result of the original tubal ligation, or the reversal of the tubal ligation. (amazonaws.com)
  • The success of the procedure was confirmed October 8, 2008, by a patient who came to Lakeshore Surgical Center in March, 2008, to be one of the first women in the country to have the Essure Reversal performed by expert tubal ligation reversal surgeons, Dr. William Greene Jr. and Dr. Wendell Turner. (medicalnewstoday.com)
  • Lakeshore Surgical Center is the leading specialized Tubal Ligation Reversal center. (medicalnewstoday.com)
  • A tubal ligation reversal is a procedure to restore fertility after a woman has had a tubal ligation - a procedure that cuts or blocks the fallopian tubes to prevent pregnancy. (mayoclinic.org)
  • During a tubal ligation reversal, the blocked segments of the fallopian tubes are reconnected to the remainder of the fallopian tubes. (mayoclinic.org)
  • Sterilization procedures that cause the least amount of damage to the fallopian tubes are the most likely to allow successful tubal ligation reversal. (mayoclinic.org)
  • In cases where tubal ligation reversal isn't recommended, in vitro fertilization (IVF) may be an option. (mayoclinic.org)
  • A tubal ligation reversal may allow a woman who had a tubal ligation to get pregnant without further medical assistance. (mayoclinic.org)
  • A tubal ligation reversal isn't appropriate for everyone. (mayoclinic.org)
  • A tubal ligation reversal is more likely to be successful if there is still a large proportion of healthy tube. (mayoclinic.org)
  • If your fallopian tubes were originally blocked by clips or rings, the tubal ligation reversal is more likely to be successful than if segments of your fallopian tubes were burned in order to close them off (electrocautery). (mayoclinic.org)
  • Even if tubal ligation reversal is successful, it doesn't guarantee that you can become pregnant. (mayoclinic.org)
  • Pregnancy rates following reversal of tubal ligation vary greatly depending on a woman's age and other factors. (mayoclinic.org)
  • Tubal ligation reversal is abdominal surgery, which carries a risk of infection, bleeding and injury to nearby organs, as well as risks related to anesthesia. (mayoclinic.org)
  • If you do conceive after having a tubal ligation reversal, there's a chance that the pregnancy will be ectopic - when the fertilized egg implants outside the uterus, usually in a fallopian tube. (mayoclinic.org)
  • A tubal ligation reversal can be done as an inpatient or outpatient procedure. (mayoclinic.org)
  • To do a tubal ligation reversal, your doctor will make a small incision in your abdomen (minilaparotomy) and expose your uterus, fallopian tubes and ovaries. (mayoclinic.org)
  • Avoid using aspirin for pain relief after tubal ligation reversal, since it may promote bleeding. (mayoclinic.org)
  • What Is Tubal Ligation Reversal and How Successful Is It? (healthline.com)
  • A tubal ligation reversal reconnects the blocked or cut segments of the fallopian tube. (healthline.com)
  • If you have one of these conditions, your doctor might take that into account when deciding if a tubal ligation reversal is right for you. (healthline.com)
  • Having general fertility problems makes tubal ligation reversal less successful. (healthline.com)
  • The average cost of a tubal ligation reversal in the United States is $8,685. (healthline.com)
  • In general, 50 to 80 percent of women who have tubal ligation reversal go on to have successful pregnancies. (healthline.com)
  • Tubal reversal is microsurgery to repair the fallopian tube after a tubal ligation procedure. (bionity.com)
  • However, a tubal ligation reversal procedure can be used to reconnect the fallopian tubes. (americanpregnancy.org)
  • It is also important to be aware that tubal ligation reversal increases the chances of an ectopic pregnancy. (americanpregnancy.org)
  • A tubal ligation operative report is useful as a guide but does not predict exactly what the remaining tubal segments will be at the time of a reversal procedure. (tubal-reversal.net)
  • At A Personal Choice, we keep a record of all patients' tubal reversal operations, including the tubal ligation method, the remaining tubal segment lengths, and other important findings at the time of surgery. (tubal-reversal.net)
  • The overall pregnancy rate after Pomeroy tubal ligation reversal is 70% for patients at A Personal Choice. (tubal-reversal.net)
  • From the follow-up data that we have for our tubal reversal patients, there is good reason for optimism when reversing a Pomeroy tubal ligation. (tubal-reversal.net)
  • In this case, a tubal ligation reversal can be attempted. (wisegeek.com)
  • A Personal Choice Tubal Reversal Center is in Raleigh, North Carolina and specializes in tubal ligation, Essure, and vasectomy reversal surgery. (tubal-reversal.net)
  • Women with their tubes tied need no longer worry about being infertile as tubal ligation reversal can bail them out. (targetwoman.com)
  • 25% of the women who have undergone tubal ligation opt for tubal reversal within five years. (targetwoman.com)
  • Tubal ligation reversal or tubal sterilization reversal is termed as tubal reversal in short. (targetwoman.com)
  • This surgery is usually performed by a reproductive surgeon who has specialized and has gained thorough knowledge in tubal ligation reversal techniques. (targetwoman.com)
  • While settling in for a tubal reversal process it is advisable for the person to provide all related records pertaining to tubal ligation undergone by that person. (targetwoman.com)
  • What is tubal ligation reversal (TLR)? (brighamandwomens.org)
  • although some women have a successful reversal of a tubal ligation, this is not guaranteed. (kiwifamilies.co.nz)
  • The Essure procedure is a new type of Tubal Ligation, for women who want a permanent type of birth control or sterilization. (medicalnewstoday.com)
  • Women undergoing another type of tubal ligation, which is called coagulation or bi-polar cauterization, are again at a greater risk of developing post tubal ligation syndrome. (healthguidance.org)
  • The most common type of tubal ligation is the Pomeroy procedure , named after Dr. Ralph Pomeroy who described it in 1930. (tubal-reversal.net)
  • Here are some of the pregnancy statistics for women who have had a Pomeroy type of tubal ligation. (tubal-reversal.net)
  • The success rates of these procedures vary, depending on several things, including the type of tubal ligation surgery performed. (wisegeek.com)
  • The success of TLR will depend upon the woman's age, type of tubal ligation originally performed and the length of her remaining healthy tubes. (brighamandwomens.org)
  • If you're not having a tubal ligation shortly after childbirth or during a C-section, consider using contraception for at least one month before the procedure and continue using a reliable form of contraception until your tubal ligation procedure is performed. (mayoclinic.org)
  • Even though your chances are small, and several risks are involved in trying become pregnant after having a tubal ligation, you are not alone in the world. (amazonaws.com)
  • Tubal ligation (commonly known as having one's " tubes tied ") is a surgical procedure for female sterilization in which the fallopian tubes are permanently blocked or removed. (wikipedia.org)
  • Most tubal ligation procedures involve accessing the abdominal cavity through incisions in the abdominal wall and require some form of regional or general anesthesia (see Procedure technique below). (wikipedia.org)
  • In addition, a bilateral salpingectomy is effective as a tubal ligation procedure. (princeton.edu)
  • Tubal ligation is a surgical procedure that is used as a means to achieve (mostly) permanent infertility in women. (everything2.com)
  • If you have an interval tubal ligation as an outpatient procedure, either a needle is inserted or an incision is made through your belly button so your abdomen can be inflated with gas (carbon dioxide or nitrous oxide). (mayoclinic.org)
  • A tubal ligation is a procedure that will interrupt the patency of the fallopian tubes, as a preventative measure against future pregnancies. (amazonaws.com)
  • In the United States, about 10 million women have had their tubes tied -- a procedure called tubal ligation -- as a permanent form of birth control since the 1960s, according to the Centers for Disease Control and Prevention (CDC) National Center for Health Statistics. (medicinenet.com)
  • Tubal ligation is surgical procedure to prevent pregnancy. (hopkinsmedicine.org)
  • Even though tubal ligation is a safe and effective form of birth control, about 1 out of 200 women may still become pregnant after the procedure. (hopkinsmedicine.org)
  • The Essure procedure differs from other Tubal Ligation due to the fact that a coil-like device is inserted into the fallopian tube where it meets the uterus. (medicalnewstoday.com)
  • Tubal Ligation Reversals have been performed for years, but the Essure procedure, which is stated to be non-reversible, has not been successfully reversed before now. (medicalnewstoday.com)
  • Laparoscopic tubal ligation is a surgical sterilization procedure in which a woman's fallopian tubes are either clamped and blocked or severed and sealed. (medscape.com)
  • Laparoscopic tubal ligation has many advantages that explain its use as the interval procedure of choice. (medscape.com)
  • This is often combined with ligation: a procedure in which the varicose vein is tied off or "ligated" before its removal. (medic8.com)
  • What is the Effectiveness of a Tubal Ligation Procedure? (plannedparenthood.org)
  • A number of prospective studies have found rubber-band ligation to be a simple, safe, and effective method for treating symptomatic first-, second-, and third-degree hemorrhoids as an outpatient procedure with significant improvement in quality of life. (medscape.com)
  • A tubal ligation is a sterilization procedure to close the tubes. (denverhealth.org)
  • Tubal ligation can be performed under either general anesthesia or local anesthesia (spinal or epidural, often supplemented witha tranquilizer to calm the patient during the procedure). (bionity.com)
  • In a small percentage of cases, a tubal ligation procedure leaves only the distal portion of the fallopian tube and no proximal tubal opening into the uterus. (bionity.com)
  • Successful repair of the fallopian tubes is now possible in 98% of women who have had a tubal ligation, regardless of the type of sterilization procedure. (bionity.com)
  • Tubal ligation is a procedure that one in four married couples will choose as a form of birth control. (americanpregnancy.org)
  • Currently, many doctors use the term modified Pomeroy procedure when they describe the tubal ligation they have performed in a patient's operative report. (tubal-reversal.net)
  • A tubal ligation with rings is a very similar procedure, but stretchy silicone rings are used to section off a loop in the fallopian tube instead. (wisegeek.com)
  • Although a tubal ligation is often considered a permanent female contraception, some women may want to have children after the procedure is done. (wisegeek.com)
  • Tubal ligation is surgical procedure you may choose if you are an adult woman and you do not want to get pregnant in the future. (stanfordchildrens.org)
  • Tubal ligation is a safe procedure, but all surgeries carry some risks. (stanfordchildrens.org)
  • If a product is to be inserted, such as in a cloning procedure, an excess of insert of 2 to 3 x the vector concentration is used, and the concentration of DNA is higher to increase the occurrences of intermolecular ligation. (rice.edu)
  • ElizaBennett - My understanding is that vasectomy is a less invasive procedure with a shorter recovery time than tubal ligation. (wisegeek.com)
  • Conclusions: Rubber band ligation is a rapid and safe non operative procedure for treating 1st, 2nd and early 3rd degree haemorrhoids on out patient basis. (thefreelibrary.com)
  • Rubber band ligation is a simple and safe procedure which is performed in outpatient department and leads to resolution of symptoms and effectively cures haemorrhoids2,5,6. (thefreelibrary.com)
  • Belcher says her struggle to find an answer has been difficult because many medical experts say that post-tubal ligation syndrome does not exist. (medicinenet.com)
  • However, allegations that the surgery could lead to post-tubal ligation syndrome first surfaced in the 1950s. (medicinenet.com)
  • According to Corson and other doctors looking into post-tubal ligation "syndrome," it may actually be a combination of discontinuing the use of birth control pills and age-related factors. (medicinenet.com)
  • Given these effects, many of the symptoms reported by women claiming to have post-tubal ligation syndrome could, in fact, be a result of coming off the pill rather than a result of the surgery. (medicinenet.com)
  • Though most of the women who undergo tubal ligation do not suffer from any side effects, it is seen that only in 37% only of the women suffer from post tubal ligation syndrome. (healthguidance.org)
  • According to some of the studies conducted, there are some women who are considered to be at a greater risk of post tubal ligation syndrome as compared to the others. (healthguidance.org)
  • Let's see some facts that can educate us on who can be at a risk of developing post tubal ligation syndrome and what are the symptoms of this syndrome. (healthguidance.org)
  • Women who have their tubes tied through the process of cauterization are at a higher risk of developing post tubal ligation syndrome as compared to those women who have then tied by clips or rings. (healthguidance.org)
  • As explained by doctors' cases of mid-segment tubal ligations are more prone to post tubal ligation syndrome. (healthguidance.org)
  • The reason that women develop post tubal ligation syndrome after having the fallopian tubes tied is the marked decline in the estrogen and the progesterone levels in the body. (healthguidance.org)
  • If you have undergone a tubal ligation and are suffering from any of the symptoms mentioned above, then it is quite possible that you are suffering from post tubal ligation syndrome. (healthguidance.org)
  • Female sterilization can cause negative iatrogenic side effects known by the OBGYN medical community as post tubal ligation syndrome (PTLS) or post sterilization syndrome . (change.org)
  • While female sterilization procedures are highly effective at preventing pregnancy, there is a small continuing risk of unintended pregnancy after tubal ligation. (wikipedia.org)
  • About 1 out of 200 women who have had tubal ligation get pregnant later. (medlineplus.gov)
  • Your doctor may not be able to reattach one or both of your fallopian tubes if too much was removed during the tubal ligation. (mayoclinic.org)
  • Tubal ligation or tubectomy (informally known as getting one's "tubes tied") is a form of female sterilization , in which the fallopian tubes are severed and sealed or "pinched shut", in order to prevent fertilization . (princeton.edu)
  • Tubal ligation (getting your "tubes tied") is a type of surgery. (medlineplus.gov)
  • Tubal ligation - also known as having your tubes tied or tubal sterilization - is a type of permanent birth control. (mayoclinic.org)
  • In a tubal ligation , also known as "having your tubes tied," your fallopian tubes are cut or blocked. (healthline.com)
  • Otherwise known as "having your tubes tied," tubal ligation involves cutting and tying off the fallopian tubes to prevent eggs from entering the uterus. (americanpregnancy.org)
  • There are quite a number of women who prefer to undergo sterilization called as tubal ligation, which means that they have their fallopian tubes tied up. (healthguidance.org)
  • You may have heard tubal ligation referred to as "getting your tubes tied. (stanfordchildrens.org)
  • Tubal sterilisation (sometimes referred to as tubal ligation or 'having your tubes tied') is a permanent method of contraception. (vic.gov.au)
  • Tubal sterilisation, also known as tubal ligation or 'having your tubes tied', is a permanent method of contraception that you can choose if you are sure that you do not want to have children in the future. (vic.gov.au)
  • Tubal ligation, or having "tubes tied," is performed to prevent pregnancy and is a common form of contraception. (brighamandwomens.org)
  • Less commonly, tubal ligation procedures may also be performed for patients who are known to be carriers of genes that increase the risk of ovarian and fallopian tube cancer, such as BRCA1 and BRCA2 . (wikipedia.org)
  • Partial tubal ligation or full salpingectomy reduces the lifetime risk of developing ovarian or fallopian tube cancer later in life. (wikipedia.org)
  • There are mainly four occlusion methods for tubal ligation, typically carried out on the isthmic portion of the fallopian tube , that is, the thin portion of the tube closest to the uterus. (princeton.edu)
  • If you had a tubal ligation and you feel you are pregnant, call your doctor right away to make sure the pregnancy is not in the fallopian tube (ectopic pregnancy). (amazonaws.com)
  • It has also been noted that most pregnancies occurring after ligation are ectopic in nature, meaning that the pregnancy occurs at the fallopian tube instead of the uterus. (babycenter.com)
  • Fertilization happens in the fallopian tube, so a tubal ligation prevents pregnancy by keeping the sperm and egg from meeting. (healthline.com)
  • If your doctor sees that you have enough fallopian tube left to reverse your tubal ligation, and everything else looks healthy, they'll perform the surgery. (healthline.com)
  • During a Pomeroy tubal ligation , a small loop is tied in the middle of the fallopian tube and stitched in place. (wisegeek.com)
  • [ 1 ] Rubber-band ligation is one of the most common outpatient treatments available for patients with hemorrhoids. (medscape.com)
  • Rubber-band ligation is readily performed in an ambulatory setting. (medscape.com)
  • [ 7 ] Band ligation may also be considered for bleeding in severely anemic patients with fourth-degree hemorrhoids who are unfit for surgery. (medscape.com)
  • Ambulatory treatments for hemorrhoids include injection sclerotherapy, rubber-band ligation, cryosurgery, infrared coagulation, and ultrasonic Doppler-guided transanal hemorrhoidal artery ligation (HAL). (medscape.com)
  • The SmartBand multi-band ligation system is used for banding esophageal varices, including bleeding esophageal varices, at or above the gastroesophageal junction and ligating internal hemorrhoids. (steris.com)
  • This endoscopic band ligation system offers both a complete endoscopic ligation kit and a reloading pack for those cases where extra bands are required. (steris.com)
  • Endoscopic band ligation is the use of elastic bands to treat the varices. (medicalcityhospital.com)
  • Experience of rubber band ligation for haemorrhoidal disease. (thefreelibrary.com)
  • Objectives: To assess symptomatic relief in haemorrhoidal disease using rubber band ligation. (thefreelibrary.com)
  • Only patients with 1st, 2nd and 3rd degree haemorrhoids (bleeding and prolapse) were selected for the study and they underwent rubber band ligation in two sessions, 6 weeks apart. (thefreelibrary.com)
  • Results: A total of 105 patients underwent rubber band ligation. (thefreelibrary.com)
  • At 3 months of follow up rubber band ligation cured 76(72.4%) patients, improved 13(12.4%) and failed in 7(6.7%) patients. (thefreelibrary.com)
  • A number of non operative methods are practiced for non prolapsing and early prolapsing haemorrhoids, these include Barron elastic band ligation, infrared coagulation, injection sclerotherapy and cryotherapy3. (thefreelibrary.com)
  • Rubber band ligation works well for patient with 2nd degree internal haemorrhoids but less well for patients with 3rd degree external haemorrhoids7. (thefreelibrary.com)
  • This technique has also been described as "expressed protein ligation" (4,5). (neb.com)
  • We exploited intein-mediated protein ligation to produce a semisynthetic ribonuclease A. Of the 124 residues of RNase A, residues 1-94 were linked to an intein. (hindawi.com)
  • Laparoscopic tubal ligation is attractive to surgeons because many gynecologists are well trained in laparoscopic techniques and it involves a short operating time. (medscape.com)
  • Disadvantages of laparoscopic tubal ligation include the need for anesthesia and cost of laparoscopic equipment. (medscape.com)
  • For a laparoscopic tubal ligation , the surgeon makes two small cuts (incisions)-one in or just below the belly button (navel) and one lower on your abdomen. (healthlinkbc.ca)
  • Laparoscopic tubal ligation leaves small scars. (healthlinkbc.ca)
  • That is nearly four times the estimated risk after a laparoscopic tubal ligation, the more traditional method. (chicagotribune.com)
  • During a laparoscopic tubal ligation, a physician makes a small cut in the abdomen, then blocks, ties or seals the fallopian tubes to keep eggs and sperm apart. (chicagotribune.com)
  • For laparoscopic tubal ligations, the pregnancy risks were significantly lower: 24 to 30 pregnancies per 1,000 women. (chicagotribune.com)
  • Studies have shown that tubal ligation (tying of the fallopian tubes) is not a 100% guarantee of contraception. (babycenter.com)
  • About 20% of women choose tubal ligation as their contraceptive method, making it the second most common form of female contraception in the United States. (medscape.com)
  • [2] Faith-based medical institutions in developed countries will sometimes refuse to perform tubal ligations [3] , and where long waiting times persist, there is a worrying risk of pregnancy or complications due to alternative contraception. (bionity.com)
  • Female sterilization through tubal ligation is primarily used to permanently prevent a patient from having a spontaneous pregnancy (as opposed to pregnancy via in vitro fertilization ) in the future. (wikipedia.org)
  • Tubal ligation (sometimes called female sterilization) is one of the most effective kinds of birth control. (plannedparenthood.org)
  • Vein ligation and stripping is a minor surgery. (lmh.org)
  • Following vein ligation and stripping to treat large varicose veins, you may have sclerotherapy to treat smaller varicose veins. (lmh.org)
  • Vein ligation and stripping removes varicose veins successfully in 80 out of 100 people. (lmh.org)
  • Vein ligation and stripping surgery has some risks, such as scarring and varicose veins recurring. (lmh.org)
  • While both hysterectomy (the removal of the uterus) or bilateral oophorectomy (the removal of both ovaries) can also accomplish this goal, these surgeries carry generally greater health risks than tubal ligation procedures. (wikipedia.org)
  • Having tubal ligation just after your period starts may avoid the possibility that an already fertilized egg will reach your uterus after surgery. (stanfordchildrens.org)
  • Tubal ligation is a surgery to close a woman's fallopian tubes so eggs released from the ovaries cannot enter the uterus and sperm cannot reach the egg. (brighamandwomens.org)
  • oldest form of surgical sterilization, tubal ligation, remains one of the most widely used. (britannica.com)
  • Tubal ligation is one of the most commonly used surgical sterilization procedures for women. (mayoclinic.org)
  • Tubal ligation (Female surgical sterilization). (americanpregnancy.org)
  • However, some women who choose tubal ligation regret the decision later. (medlineplus.gov)
  • Interval tubal ligation is not done after a recent delivery. (princeton.edu)
  • Also, a distinction is made between postpartum tubal ligation and interval tubal ligation , the latter not being done after a recent delivery. (bionity.com)
  • This proximity ligation assay detects zeptomole (40 x 10(-21) mol) amounts of the cytokine platelet-derived growth factor (PDGF) without washes or separations, and the mechanism can be generalized to other forms of protein analysis. (nih.gov)
  • Here, we evaluated the use of an alternative approach that uses the proximity ligation assay (PLA) to identify the presentation of an MHC class II-restricted antigen in paraffin-embedded tissue sections from patients with brain tumors. (jci.org)
  • The IPL reaction applies the chemistry described for "native chemical ligation" which fuses two synthetic peptides when the N-terminal cysteine of one peptide attacks a C-terminal thioester of another peptide (2,3). (neb.com)
  • Native chemical ligation isn't just for building big peptides anymore.The reaction, which forms amide bonds in water without enzymes, has also been used to construct nucleic acids. (acs.org)
  • Tubal ligation is surgery to close a woman's fallopian tubes. (medlineplus.gov)
  • Tubal ligation can be done at any time, including after childbirth or in combination with another abdominal surgery, such as a C-section. (mayoclinic.org)
  • Getting pregnant after a tubal ligation is possible but requires additional surgery or procedures such as in-vitro fertilization IVF. (amazonaws.com)
  • If you decide to try to try getting pregnant after a tubal ligation, you do have some options and courses of actions you can take, but they are very expensive and some involve major surgery. (amazonaws.com)
  • Corson led a study that compared hormone levels in women who had had tubal ligation versus those of women who had not had the surgery. (medicinenet.com)
  • With the introduction in the 1970s of laparoscopy (the so-called "belly button surgery"), which was less invasive than previous surgeries, more women than ever before chose tubal ligation, and reports of postoperative symptoms increased, says Corson. (medicinenet.com)
  • Located in Gainesville, GA, surgeons Dr. William A.C. Greene, Jr. and Dr. Wendell Turner have combined 50 years of outpatient gynecologic surgery experience and have performed thousands of Tubal Ligation Reversals. (medicalnewstoday.com)
  • Medtronic provides the following review of a publication comparing the use of absorbable ligation clip to metal and absorbable latch-closure clips in laparoscopic surgery. (medtronic.com)
  • Most tubal ligation reversals are done with laparoscopic surgery. (healthline.com)
  • If you have had tubal ligation surgery in the past and believe that you are currently experiencing pregnancy symptoms , talk to your health care provider. (americanpregnancy.org)
  • My physician, an ob-gyn who performed both the tubal ligation and the subsequent surgery, told me that a tubal ligation is not guaranteed 100 percent-that the chances are about one in 400 or 500 that this could happen to a woman. (chicagotribune.com)
  • What are the Different Types of Tubal Ligation Surgery? (wisegeek.com)
  • Women who do not wish to have children, or do not wish to have more children, can have a tubal ligation surgery. (wisegeek.com)
  • A partial salpinectomy is the most common method used during a tubal ligation surgery. (wisegeek.com)
  • Another less common tubal ligation surgery is known a fimbriectomy. (wisegeek.com)
  • Cauterization is another method used during a tubal ligation surgery. (wisegeek.com)
  • A tubal ligation surgery is often performed postpartum, or right after a woman gives birth. (wisegeek.com)
  • Tubal ligation surgeries can also be performed during another abdominal surgery , when a much larger incision is made. (wisegeek.com)
  • During tubal ligation surgery, both fallopian tubes are blocked or cut. (stanfordchildrens.org)
  • If you have diabetes or a history of previous abdominal surgery, pelvic inflammatory disease, or lung disease, or are overweight, you may have a higher risk for problems after tubal ligation. (stanfordchildrens.org)
  • Tubal ligation is also a short surgery, but it requires spinal or general anesthesia and there is a greater risk of complications. (wisegeek.com)
  • Patent ductus arteriosus (PDA) ligation is surgery to close the opening between your baby's aorta and pulmonary artery. (drugs.com)
  • The default in tubal ligations following on from cesarean birth is usually spinal/epidural, while the default in non-childbirth related situations may be general anesthesia as a matter of doctor preference. (bionity.com)
  • However, tubal ligations under local anesthesia, either inpatient or outpatient, may be performed under patient request. (bionity.com)
  • Like all laparoscopic procedures, tubal ligation carries the risk of complications such as damage to the major blood vessels, bowel or bladder infections, or hemorrhage. (medicinenet.com)
  • Tubal ligation may be recommended for adult women who are sure they do not want to get pregnant in the future. (medlineplus.gov)
  • More than half of women who have their tubal ligation reversed are able to become pregnant. (medlineplus.gov)
  • Getting pregnant after a tubal ligation involves certain risks including a significantly increased risk of an ectopic pregnancy. (amazonaws.com)
  • Even still, if you decide to try to become pregnant after you have had a tubal ligation, you do have some options and courses of actions you can take. (amazonaws.com)
  • I had a tubal ligation 10 months ago and while I have heard of women getting pregnant 10 yrs later, I didnt think it was possible so soon. (medhelp.org)
  • Although the possibility of becoming pregnant after tubal ligation is low, the chance is still there. (americanpregnancy.org)
  • whatever plans that we are going to make and this plan is not God's plan, then, it will not really happen.No matter how many times your tubes are being tied, if it's God's will that you will get pregnant nobody and nothing can stop God's plan even tubal ligation. (womens-health.co.uk)
  • Please advise her that even after a woman has had a tubal ligation, she can become pregnant. (chicagotribune.com)
  • You should consider tubal ligation as a permanent decision not to become pregnant. (stanfordchildrens.org)
  • Although tubal ligation may be reversed by another operation, only about 50 to 80 percent of women are able to become pregnant after having their fallopian tubes reattached. (stanfordchildrens.org)
  • Even though tubal ligation is a safe and effective form of birth control, about one out of 200 women may still become pregnant after tubal ligation. (stanfordchildrens.org)
  • Is getting pregnant after tubal ligation less common than pregnancy after vasectomy? (wisegeek.com)
  • If a woman falls pregnant after a tubal ligation, she is at an increased risk of an ectopic pregnancy (where the fertilised egg implants outside of the womb). (kiwifamilies.co.nz)
  • 1-3 The QuickLink™ DNA Ligation Kit has been optimized for utilization of T4 DNA ligase for rapid, efficient blunt and cohesive ligations performed at room temperature. (sigmaaldrich.com)
  • Both blunt and cohesive end DNA ligation, as well as single-stranded nick repair of DNA, RNA and DNA/RNA, are possible via the T4 DNA ligase. (clontech.com)
  • What are the risks of a tubal ligation? (hopkinsmedicine.org)
  • Tubal ligation is safe, but all surgeries carry some risks. (hopkinsmedicine.org)
  • Surgical ligation of patent ductus arteriosus (PDA) in newborns was associated with half the mortality risk of treatment with medication but was also associated with increased risks for neurodevelopmental, lung, and retinal deficits, according to a meta-analyses of studies that generally failed to account for confounders or adjust for survival bias. (medscape.com)
  • My previous two deliveries were through cesarean sections so Automatically this third one should be by c section accompanied By a bilateral tubal ligation as future pregnancies may be dangerous. (ewtn.com)
  • If a modified Pomeroy bilateral tubal ligation was performed, does that mean the tubes were cut and burned? (tubal-reversal.net)
  • If recircularization (intramolecular ligation) is the goal, the concentration of fragments is kept low to decrease the probability of two different molecules contacting each other. (rice.edu)
  • A method for preparing linear oligomers of duplex DNA comprising enzymatically ligating duplex DNA substrate end to end in the presence of a sufficiently high concentration of a non-specific volume-excluding polymer to promote intermolecular ligation. (google.es)
  • A slidable kit for endoscopic ligation of gastric and esophageal varices comprises an endoscope-inserting cylinder and a ligating device coaxially and integrally secured to the endoscope-inserting cylinder, where the ligating device is in such a structure that a slide cylinder having an annular rib on. (google.com.au)
  • Comparison and characterization of ulcerations induced by endoscopic ligation of esophageal varices versus endoscopic sclerotherapy. (thefreedictionary.com)
  • Endoscopic ligation of esophageal varices for prophylaxisof first bleeding in children and adolescents with portal hypertension: preliminary results of a prospective study. (thefreedictionary.com)
  • Nadolol plus isosorbide mononitrate is associated with a lower risk of rebleeding of esophageal varices in cirrhotic patients than is endoscopic ligation , reported Dr. (thefreedictionary.com)
  • We then show how this bias could affect the base composition of ancient DNA libraries prepared following AT-overhang ligation, mainly by limiting the ability to ligate DNA templates starting with thymines and therefore deaminated cytosines. (nih.gov)
  • Women who have a tubal ligation have a decreased risk of developing ovarian cancer. (medlineplus.gov)
  • Tubal ligation is an operation that involves making incisions in your abdomen. (mayoclinic.org)
  • Vein stripping and ligation, which involves tying veins shut and removing them through small cuts in your skin, is usually performed only in severe cases of varicose veins. (webmd.com)
  • Increased risk of a tubal (ectopic) pregnancy if pregnancy occurs after a tubal ligation. (medlineplus.gov)
  • in females sterilization results from tubal ligation-the blocking or severing of the fallopian tubes, which transport the female gamete and provide an environment for fertilization. (britannica.com)
  • In this webinar, NEB Scientist and ligase expert Greg Lohman discusses mismatch ligation by DNA ligases and the molecular diagnostics applications that depend on the use of high-fidelity DNA ligases like NEB's HiFi Taq DNA Ligase to detect single base differences in DNA. (neb.com)
  • If the total volume of DNA is greater than 10 µl, the volume of the ligation buffer and the ligase should be adjusted accordingly, and the incubation time should be extended to 20-30 minutes. (sigmaaldrich.com)
  • The rate of blunt-end ligation of DNA substrate by T4 DNA ligase is particularly affected, with a product shift from closed. (google.es)
  • The rate of blunt-end ligation of DNA substrate by T4 DNA ligase is particularly affected, with a product shift from closed circular species to linear oligomers. (google.es)
  • 1. A method of enzymatically ligating DNA substrates comprising intermolecularly ligating between ends of said substrate or intramolecularly ligating two ends of one DNA molecule by a DNA ligase in the presence of a sufficiently high concentration of a non-specific, volume-excluding polymer which does not specifically interact with the DNA substrate, the DNA ligase or the DNA product, to obtain an increased ligation rate. (google.es)
  • Commonly known as the OLA, the Oligonucleotide Ligation Assay is an analyte specific reagent (ASR) that is centered around the hybridization of a PCR primer with an exact match to a target sequence. (thefreedictionary.com)
  • The systems we evaluated included (a) CF oligonucleotide ligation assay (OLA), Ver. (thefreedictionary.com)
  • Mutation in the gene coding for coagulation factor V and resistance to activated protein C: detection of the genetic mutation by oligonucleotide ligation assay using a semi-automated system. (thefreedictionary.com)
  • Other publications demonstrated that the oligonucleotide ligation assay (36) and single base chain extension (32) could be used for genotyping DNA samples with the microbead approach. (thefreedictionary.com)
  • A technology termed " oligonucleotide ligation assay " uses target-specific ligation to generate mutation-specific products that are analyzed by gel electrophoresis (2). (thefreedictionary.com)
  • Traditional methods for the identification of known polymorphisms are restriction fragment-length polymorphism (RFLP) analysis, oligonucleotide ligation assay , and minisequencing (38-41). (thefreedictionary.com)
  • The oligonucleotide ligation assay (OLA) relies on hybridization with specific oligonucleotide probes that can effectively discriminate between the wild-type and variant sequences. (thefreedictionary.com)
  • Cutting-edge and thorough, Enzyme-Mediated Ligation Methods is a valuable resource for students and scientists from different disciplines who are interested in using enzymatic strategies to answer their research questions. (springer.com)
  • See Tubal ligation methods below. (wikipedia.org)
  • Many other detection methods capitalizing on the ligation of DNA are being employed by basic and applied research groups, as well as many molecular diagnostics (MDx) companies. (neb.com)
  • The kit is used for the ligation of DNA fragments produced by restriction digestion, PCR, or other physical/enzymatic methods. (sigmaaldrich.com)
  • Some minimal invasive procedures, such as endoscopic ligation or sclerotherapy, [sup][5] or splenic artery embolization can be selected when drug therapy failed. (thefreedictionary.com)
  • iii) endoscopic variceal ligation had a significantly lower rate of development of portal gastropathy and re-bleeding, (iv) while both techniques influenced gastric varices equally, there was significantly higher oesophageal variceal recurrence after EVL than sclerotherapy. (thefreedictionary.com)
  • Endoscopic ligation compared with sclerotherapy for bleeding varices in children with extra-hepatic portal venous obstruction. (thefreedictionary.com)
  • 50) The results of this study using sclerotherapy serve as the reference level for comparison by future endoscopic ligation studies. (thefreedictionary.com)
  • During tubal ligation, the fallopian tubes are cut, tied or blocked to permanently prevent pregnancy. (mayoclinic.org)
  • Tubal ligation is one of the most effective ways to prevent pregnancy, with rates of pregnancy around 1/1,000 after the first year , and between 2-10/1,000 after five years . (americanpregnancy.org)
  • Tubal ligation is done in a hospital or outpatient clinic. (medlineplus.gov)
  • Vein stripping and ligation takes about two to three hours and is usually done in an outpatient setting. (webmd.com)
  • approximately one third of pregnancies that occur after a tubal ligation will be ectopic pregnancies. (wikipedia.org)
  • Fortunately, Pomeroy tubal ligation and its modifications are usually excellent in terms of reversibility. (tubal-reversal.net)