White blood cells. These include granular leukocytes (BASOPHILS; EOSINOPHILS; and NEUTROPHILS) as well as non-granular leukocytes (LYMPHOCYTES and MONOCYTES).
Granular leukocytes having a nucleus with three to five lobes connected by slender threads of chromatin, and cytoplasm containing fine inconspicuous granules and stainable by neutral dyes.
Mature LYMPHOCYTES and MONOCYTES transported by the blood to the body's extravascular space. They are morphologically distinguishable from mature granulocytic leukocytes by their large, non-lobed nuclei and lack of coarse, heavily stained cytoplasmic granules.
The movement of leukocytes in response to a chemical concentration gradient or to products formed in an immunologic reaction.
The engulfing and degradation of microorganisms; other cells that are dead, dying, or pathogenic; and foreign particles by phagocytic cells (PHAGOCYTES).
The number of WHITE BLOOD CELLS per unit volume in venous BLOOD. A differential leukocyte count measures the relative numbers of the different types of white cells.
Adherence of cells to surfaces or to other cells.
A formylated tripeptide originally isolated from bacterial filtrates that is positively chemotactic to polymorphonuclear leucocytes, and causes them to release lysosomal enzymes and become metabolically activated.
Cell adhesion molecule and CD antigen that mediates the adhesion of neutrophils and monocytes to activated platelets and endothelial cells.
Large, phagocytic mononuclear leukocytes produced in the vertebrate BONE MARROW and released into the BLOOD; contain a large, oval or somewhat indented nucleus surrounded by voluminous cytoplasm and numerous organelles.
The minute vessels that collect blood from the capillary plexuses and join together to form veins.
Cell-surface glycoprotein beta-chains that are non-covalently linked to specific alpha-chains of the CD11 family of leukocyte-adhesion molecules (RECEPTORS, LEUKOCYTE-ADHESION). A defect in the gene encoding CD18 causes LEUKOCYTE-ADHESION DEFICIENCY SYNDROME.
A pathological process characterized by injury or destruction of tissues caused by a variety of cytologic and chemical reactions. It is usually manifested by typical signs of pain, heat, redness, swelling, and loss of function.
The natural bactericidal property of BLOOD due to normally occurring antibacterial substances such as beta lysin, leukin, etc. This activity needs to be distinguished from the bactericidal activity contained in a patient's serum as a result of antimicrobial therapy, which is measured by a SERUM BACTERICIDAL TEST.
Disorders in which phagocytic cells cannot kill ingested bacteria; characterized by frequent recurring infection with formulation of granulomas.
Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others.
White blood cells formed in the body's lymphoid tissue. The nucleus is round or ovoid with coarse, irregularly clumped chromatin while the cytoplasm is typically pale blue with azurophilic (if any) granules. Most lymphocytes can be classified as either T or B (with subpopulations of each), or NATURAL KILLER CELLS.
The process in which the neutrophil is stimulated by diverse substances, resulting in degranulation and/or generation of reactive oxygen products, and culminating in the destruction of invading pathogens. The stimulatory substances, including opsonized particles, immune complexes, and chemotactic factors, bind to specific cell-surface receptors on the neutrophil.
Technique using an instrument system for making, processing, and displaying one or more measurements on individual cells obtained from a cell suspension. Cells are usually stained with one or more fluorescent dyes specific to cell components of interest, e.g., DNA, and fluorescence of each cell is measured as it rapidly transverses the excitation beam (laser or mercury arc lamp). Fluorescence provides a quantitative measure of various biochemical and biophysical properties of the cell, as well as a basis for cell sorting. Other measurable optical parameters include light absorption and light scattering, the latter being applicable to the measurement of cell size, shape, density, granularity, and stain uptake.
Cell adhesion molecule and CD antigen that serves as a homing receptor for lymphocytes to lymph node high endothelial venules.
Leukocytes with abundant granules in the cytoplasm. They are divided into three groups according to the staining properties of the granules: neutrophilic, eosinophilic, and basophilic. Mature granulocytes are the NEUTROPHILS; EOSINOPHILS; and BASOPHILS.
The major metabolite in neutrophil polymorphonuclear leukocytes. It stimulates polymorphonuclear cell function (degranulation, formation of oxygen-centered free radicals, arachidonic acid release, and metabolism). (From Dictionary of Prostaglandins and Related Compounds, 1990)
Chemical substances that attract or repel cells. The concept denotes especially those factors released as a result of tissue injury, microbial invasion, or immunologic activity, that attract LEUKOCYTES; MACROPHAGES; or other cells to the site of infection or insult.
A cell-surface ligand involved in leukocyte adhesion and inflammation. Its production is induced by gamma-interferon and it is required for neutrophil migration into inflamed tissue.
The relatively long-lived phagocytic cell of mammalian tissues that are derived from blood MONOCYTES. Main types are PERITONEAL MACROPHAGES; ALVEOLAR MACROPHAGES; HISTIOCYTES; KUPFFER CELLS of the liver; and OSTEOCLASTS. They may further differentiate within chronic inflammatory lesions to EPITHELIOID CELLS or may fuse to form FOREIGN BODY GIANT CELLS or LANGHANS GIANT CELLS. (from The Dictionary of Cell Biology, Lackie and Dow, 3rd ed.)
Single pavement layer of cells which line the luminal surface of the entire vascular system and regulate the transport of macromolecules and blood components.
Techniques used for determining the values of photometric parameters of light resulting from LUMINESCENCE.
Surface ligands, usually glycoproteins, that mediate cell-to-cell adhesion. Their functions include the assembly and interconnection of various vertebrate systems, as well as maintenance of tissue integration, wound healing, morphogenic movements, cellular migrations, and metastasis.
Proteins that bind to particles and cells to increase susceptibility to PHAGOCYTOSIS, especially ANTIBODIES bound to EPITOPES that attach to FC RECEPTORS. COMPLEMENT C3B may also participate.
Transmembrane proteins consisting of a lectin-like domain, an epidermal growth factor-like domain, and a variable number of domains that are homologous to complement regulatory proteins. They are important cell adhesion molecules which help LEUKOCYTES attach to VASCULAR ENDOTHELIUM.
The transfer of leukocytes from a donor to a recipient or reinfusion to the donor.
A hemeprotein from leukocytes. Deficiency of this enzyme leads to a hereditary disorder coupled with disseminated moniliasis. It catalyzes the conversion of a donor and peroxide to an oxidized donor and water. EC
The species Oryctolagus cuniculus, in the family Leporidae, order LAGOMORPHA. Rabbits are born in burrows, furless, and with eyes and ears closed. In contrast with HARES, rabbits have 22 chromosome pairs.
An antiseptic with mild fungistatic, bacteriostatic, anthelmintic, and amebicidal action. It is also used as a reagent and metal chelator, as a carrier for radio-indium for diagnostic purposes, and its halogenated derivatives are used in addition as topical anti-infective agents and oral antiamebics.
An adhesion-promoting leukocyte surface membrane heterodimer. The alpha subunit consists of the CD11b ANTIGEN and the beta subunit the CD18 ANTIGEN. The antigen, which is an integrin, functions both as a receptor for complement 3 and in cell-cell and cell-substrate adhesive interactions.
Enzymes catalyzing the oxidation of arachidonic acid to hydroperoxyarachidonates. These products are then rapidly converted by a peroxidase to hydroxyeicosatetraenoic acids. The positional specificity of the enzyme reaction varies from tissue to tissue. The final lipoxygenase pathway leads to the leukotrienes. EC 1.13.11.- .
Highly reactive compounds produced when oxygen is reduced by a single electron. In biological systems, they may be generated during the normal catalytic function of a number of enzymes and during the oxidation of hemoglobin to METHEMOGLOBIN. In living organisms, SUPEROXIDE DISMUTASE protects the cell from the deleterious effects of superoxides.
Effective in the initiation of protein synthesis. The initiating methionine residue enters the ribosome as N-formylmethionyl tRNA. This process occurs in Escherichia coli and other bacteria as well as in the mitochondria of eucaryotic cells.
Cells that can carry out the process of PHAGOCYTOSIS.
Cell adhesion molecule and CD antigen that mediates neutrophil, monocyte, and memory T-cell adhesion to cytokine-activated endothelial cells. E-selectin recognizes sialylated carbohydrate groups related to the Lewis X or Lewis A family.
An enzyme that catalyzes the oxidation of arachidonic acid to yield 5-hydroperoxyarachidonate (5-HPETE) which is rapidly converted by a peroxidase to 5-hydroxy-6,8,11,14-eicosatetraenoate (5-HETE). The 5-hydroperoxides are preferentially formed in leukocytes.
5-Amino-2,3-dihydro-1,4-phthalazinedione. Substance that emits light on oxidation. It is used in chemical determinations.
Antibodies produced by a single clone of cells.
Non-nucleated disk-shaped cells formed in the megakaryocyte and found in the blood of all mammals. They are mainly involved in blood coagulation.
Elements of limited time intervals, contributing to particular results or situations.
A large increase in oxygen uptake by neutrophils and most types of tissue macrophages through activation of an NADPH-cytochrome b-dependent oxidase that reduces oxygen to a superoxide. Individuals with an inherited defect in which the oxidase that reduces oxygen to superoxide is decreased or absent (GRANULOMATOUS DISEASE, CHRONIC) often die as a result of recurrent bacterial infections.
Differentiation antigens residing on mammalian leukocytes. CD stands for cluster of differentiation, which refers to groups of monoclonal antibodies that show similar reactivity with certain subpopulations of antigens of a particular lineage or differentiation stage. The subpopulations of antigens are also known by the same CD designation.
Class of pro-inflammatory cytokines that have the ability to attract and activate leukocytes. They can be divided into at least three structural branches: C; (CHEMOKINES, C); CC; (CHEMOKINES, CC); and CXC; (CHEMOKINES, CXC); according to variations in a shared cysteine motif.
Non-antibody proteins secreted by inflammatory leukocytes and some non-leukocytic cells, that act as intercellular mediators. They differ from classical hormones in that they are produced by a number of tissue or cell types rather than by specialized glands. They generally act locally in a paracrine or autocrine rather than endocrine manner.
Serum glycoprotein produced by activated MACROPHAGES and other mammalian MONONUCLEAR LEUKOCYTES. It has necrotizing activity against tumor cell lines and increases ability to reject tumor transplants. Also known as TNF-alpha, it is only 30% homologous to TNF-beta (LYMPHOTOXIN), but they share TNF RECEPTORS.
RNA sequences that serve as templates for protein synthesis. Bacterial mRNAs are generally primary transcripts in that they do not require post-transcriptional processing. Eukaryotic mRNA is synthesized in the nucleus and must be exported to the cytoplasm for translation. Most eukaryotic mRNAs have a sequence of polyadenylic acid at the 3' end, referred to as the poly(A) tail. The function of this tail is not known for certain, but it may play a role in the export of mature mRNA from the nucleus as well as in helping stabilize some mRNA molecules by retarding their degradation in the cytoplasm.
Condensed areas of cellular material that may be bounded by a membrane.
Lipid-containing polysaccharides which are endotoxins and important group-specific antigens. They are often derived from the cell wall of gram-negative bacteria and induce immunoglobulin secretion. The lipopolysaccharide molecule consists of three parts: LIPID A, core polysaccharide, and O-specific chains (O ANTIGENS). When derived from Escherichia coli, lipopolysaccharides serve as polyclonal B-cell mitogens commonly used in laboratory immunology. (From Dorland, 28th ed)
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
The minor fragment formed when C5 convertase cleaves C5 into C5a and COMPLEMENT C5B. C5a is a 74-amino-acid glycopeptide with a carboxy-terminal ARGININE that is crucial for its spasmogenic activity. Of all the complement-derived anaphylatoxins, C5a is the most potent in mediating immediate hypersensitivity (HYPERSENSITIVITY, IMMEDIATE), smooth MUSCLE CONTRACTION; HISTAMINE RELEASE; and migration of LEUKOCYTES to site of INFLAMMATION.
A member of the CXC chemokine family that plays a role in the regulation of the acute inflammatory response. It is secreted by variety of cell types and induces CHEMOTAXIS of NEUTROPHILS and other inflammatory cells.
A metallic element, atomic number 49, atomic weight 114.82, symbol In. It is named from its blue line in the spectrum. (From Dorland, 28th ed)
Naturally occurring or experimentally induced animal diseases with pathological processes sufficiently similar to those of human diseases. They are used as study models for human diseases.
Strains of mice in which certain GENES of their GENOMES have been disrupted, or "knocked-out". To produce knockouts, using RECOMBINANT DNA technology, the normal DNA sequence of the gene being studied is altered to prevent synthesis of a normal gene product. Cloned cells in which this DNA alteration is successful are then injected into mouse EMBRYOS to produce chimeric mice. The chimeric mice are then bred to yield a strain in which all the cells of the mouse contain the disrupted gene. Knockout mice are used as EXPERIMENTAL ANIMAL MODELS for diseases (DISEASE MODELS, ANIMAL) and to clarify the functions of the genes.
Lymphocytes responsible for cell-mediated immunity. Two types have been identified - cytotoxic (T-LYMPHOCYTES, CYTOTOXIC) and helper T-lymphocytes (T-LYMPHOCYTES, HELPER-INDUCER). They are formed when lymphocytes circulate through the THYMUS GLAND and differentiate to thymocytes. When exposed to an antigen, they divide rapidly and produce large numbers of new T cells sensitized to that antigen.
Assays that measure the rate of migration of LEUKOCYTES. They may involve a variety of techniques such as measuring the movement of leukocytes through substrates such as AGAROSE gels or the rate of exit of cells from a glass capillary.
The circulation of the BLOOD through the MICROVASCULAR NETWORK.
A phospholipid derivative formed by PLATELETS; BASOPHILS; NEUTROPHILS; MONOCYTES; and MACROPHAGES. It is a potent platelet aggregating agent and inducer of systemic anaphylactic symptoms, including HYPOTENSION; THROMBOCYTOPENIA; NEUTROPENIA; and BRONCHOCONSTRICTION.
C5 plays a central role in both the classical and the alternative pathway of COMPLEMENT ACTIVATION. C5 is cleaved by C5 CONVERTASE into COMPLEMENT C5A and COMPLEMENT C5B. The smaller fragment C5a is an ANAPHYLATOXIN and mediator of inflammatory process. The major fragment C5b binds to the membrane initiating the spontaneous assembly of the late complement components, C5-C9, into the MEMBRANE ATTACK COMPLEX.
Granular leukocytes with a nucleus that usually has two lobes connected by a slender thread of chromatin, and cytoplasm containing coarse, round granules that are uniform in size and stainable by eosin.
Colorless to yellow dye that is reducible to blue or black formazan crystals by certain cells; formerly used to distinguish between nonbacterial and bacterial diseases, the latter causing neutrophils to reduce the dye; used to confirm diagnosis of chronic granulomatous disease.
The diffusion or accumulation of neutrophils in tissues or cells in response to a wide variety of substances released at the sites of inflammatory reactions.
A promyelocytic cell line derived from a patient with ACUTE PROMYELOCYTIC LEUKEMIA. HL-60 cells lack specific markers for LYMPHOID CELLS but express surface receptors for FC FRAGMENTS and COMPLEMENT SYSTEM PROTEINS. They also exhibit phagocytic activity and responsiveness to chemotactic stimuli. (From Hay et al., American Type Culture Collection, 7th ed, pp127-8)
Cytokine-induced cell adhesion molecule present on activated endothelial cells, tissue macrophages, dendritic cells, bone marrow fibroblasts, myoblasts, and myotubes. It is important for the recruitment of leukocytes to sites of inflammation. (From Pigott & Power, The Adhesion Molecule FactsBook, 1993, p154)
Established cell cultures that have the potential to propagate indefinitely.
(2S-(2 alpha,3 beta(1E,3E,5Z,8Z)))-3-(1,3,5,8-Tetradecatetraenyl)oxiranebutanoic acid. An unstable allylic epoxide, formed from the immediate precursor 5-HPETE via the stereospecific removal of a proton at C-10 and dehydration. Its biological actions are determined primarily by its metabolites, i.e., LEUKOTRIENE B4 and cysteinyl-leukotrienes. Alternatively, leukotriene A4 is converted into LEUKOTRIENE C4 by glutathione-S-transferase or into 5,6-di-HETE by the epoxide-hydrolase. (From Dictionary of Prostaglandins and Related Compounds, 1990)
An integrin heterodimer widely expressed on cells of hematopoietic origin. CD11A ANTIGEN comprises the alpha chain and the CD18 antigen (ANTIGENS, CD18) the beta chain. Lymphocyte function-associated antigen-1 is a major receptor of T-CELLS; B-CELLS; and GRANULOCYTES. It mediates the leukocyte adhesion reactions underlying cytolytic conjugate formation, helper T-cell interactions, and antibody-dependent killing by NATURAL KILLER CELLS and granulocytes. Intracellular adhesion molecule-1 has been defined as a ligand for lymphocyte function-associated antigen-1.
Red blood cells. Mature erythrocytes are non-nucleated, biconcave disks containing HEMOGLOBIN whose function is to transport OXYGEN.
Any of several ways in which living cells of an organism communicate with one another, whether by direct contact between cells or by means of chemical signals carried by neurotransmitter substances, hormones, and cyclic AMP.
Either of the pair of organs occupying the cavity of the thorax that effect the aeration of the blood.
The rate dynamics in chemical or physical systems.
INFLAMMATION of the PERITONEUM lining the ABDOMINAL CAVITY as the result of infectious, autoimmune, or chemical processes. Primary peritonitis is due to infection of the PERITONEAL CAVITY via hematogenous or lymphatic spread and without intra-abdominal source. Secondary peritonitis arises from the ABDOMINAL CAVITY itself through RUPTURE or ABSCESS of intra-abdominal organs.
An encapsulated lymphatic organ through which venous blood filters.
Proteins that are present in blood serum, including SERUM ALBUMIN; BLOOD COAGULATION FACTORS; and many other types of proteins.
Cell surface glycoproteins that bind to chemokines and thus mediate the migration of pro-inflammatory molecules. The receptors are members of the seven-transmembrane G protein-coupled receptor family. Like the CHEMOKINES themselves, the receptors can be divided into at least three structural branches: CR, CCR, and CXCR, according to variations in a shared cysteine motif.
The serous fluid of ASCITES, the accumulation of fluids in the PERITONEAL CAVITY.
A transient increase in the number of leukocytes in a body fluid.
A family of biologically active compounds derived from arachidonic acid by oxidative metabolism through the 5-lipoxygenase pathway. They participate in host defense reactions and pathophysiological conditions such as immediate hypersensitivity and inflammation. They have potent actions on many essential organs and systems, including the cardiovascular, pulmonary, and central nervous system as well as the gastrointestinal tract and the immune system.
A family of G-protein-coupled receptors that was originally identified by its ability to bind N-formyl peptides such as N-FORMYLMETHIONINE LEUCYL-PHENYLALANINE. Since N-formyl peptides are found in MITOCHONDRIA and BACTERIA, this class of receptors is believed to play a role in mediating cellular responses to cellular damage and bacterial invasion. However, non-formylated peptide ligands have also been found for this receptor class.
The movement of cells or organisms toward or away from a substance in response to its concentration gradient.
A defect of leukocyte function in which phagocytic cells ingest but fail to digest bacteria, resulting in recurring bacterial infections with granuloma formation. When chronic granulomatous disease is caused by mutations in the CYBB gene, the condition is inherited in an X-linked recessive pattern. When chronic granulomatous disease is caused by CYBA, NCF1, NCF2, or NCF4 gene mutations, the condition is inherited in an autosomal recessive pattern.
Microscopy in which television cameras are used to brighten magnified images that are otherwise too dark to be seen with the naked eye. It is used frequently in TELEPATHOLOGY.
Highly specialized EPITHELIAL CELLS that line the HEART; BLOOD VESSELS; and lymph vessels, forming the ENDOTHELIUM. They are polygonal in shape and joined together by TIGHT JUNCTIONS. The tight junctions allow for variable permeability to specific macromolecules that are transported across the endothelial layer.
A protease of broad specificity, obtained from dried pancreas. Molecular weight is approximately 25,000. The enzyme breaks down elastin, the specific protein of elastic fibers, and digests other proteins such as fibrin, hemoglobin, and albumin. EC
The cells found in the body fluid circulating throughout the CARDIOVASCULAR SYSTEM.
Glycoproteins found on the membrane or surface of cells.
A basic enzyme that is present in saliva, tears, egg white, and many animal fluids. It functions as an antibacterial agent. The enzyme catalyzes the hydrolysis of 1,4-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrin. EC
A form of phagocyte bactericidal dysfunction characterized by unusual oculocutaneous albinism, high incidence of lymphoreticular neoplasms, and recurrent pyogenic infections. In many cell types, abnormal lysosomes are present leading to defective pigment distribution and abnormal neutrophil functions. The disease is transmitted by autosomal recessive inheritance and a similar disorder occurs in the beige mouse, the Aleutian mink, and albino Hereford cattle.
A phorbol ester found in CROTON OIL with very effective tumor promoting activity. It stimulates the synthesis of both DNA and RNA.
A strong oxidizing agent used in aqueous solution as a ripening agent, bleach, and topical anti-infective. It is relatively unstable and solutions deteriorate over time unless stabilized by the addition of acetanilide or similar organic materials.
A group of three different alpha chains (CD11a, CD11b, CD11c) that are associated with an invariant CD18 beta chain (ANTIGENS, CD18). The three resulting leukocyte-adhesion molecules (RECEPTORS, LEUKOCYTE ADHESION) are LYMPHOCYTE FUNCTION-ASSOCIATED ANTIGEN-1; MACROPHAGE-1 ANTIGEN; and ANTIGEN, P150,95.
In vitro method for producing large amounts of specific DNA or RNA fragments of defined length and sequence from small amounts of short oligonucleotide flanking sequences (primers). The essential steps include thermal denaturation of the double-stranded target molecules, annealing of the primers to their complementary sequences, and extension of the annealed primers by enzymatic synthesis with DNA polymerase. The reaction is efficient, specific, and extremely sensitive. Uses for the reaction include disease diagnosis, detection of difficult-to-isolate pathogens, mutation analysis, genetic testing, DNA sequencing, and analyzing evolutionary relationships.
The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.
Exudates are fluids, CELLS, or other cellular substances that are slowly discharged from BLOOD VESSELS usually from inflamed tissues. Transudates are fluids that pass through a membrane or squeeze through tissue or into the EXTRACELLULAR SPACE of TISSUES. Transudates are thin and watery and contain few cells or PROTEINS.
A chemokine that is a chemoattractant for MONOCYTES and may also cause cellular activation of specific functions related to host defense. It is produced by LEUKOCYTES of both monocyte and lymphocyte lineage and by FIBROBLASTS during tissue injury. It has specificity for CCR2 RECEPTORS.
Microscopy using an electron beam, instead of light, to visualize the sample, thereby allowing much greater magnification. The interactions of ELECTRONS with specimens are used to provide information about the fine structure of that specimen. In TRANSMISSION ELECTRON MICROSCOPY the reactions of the electrons that are transmitted through the specimen are imaged. In SCANNING ELECTRON MICROSCOPY an electron beam falls at a non-normal angle on the specimen and the image is derived from the reactions occurring above the plane of the specimen.
Eicosatetraenoic acids substituted in any position by one or more hydroxy groups. They are important intermediates in a series of biosynthetic processes leading from arachidonic acid to a number of biologically active compounds such as prostaglandins, thromboxanes, and leukotrienes.
The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.
Toxins closely associated with the living cytoplasm or cell wall of certain microorganisms, which do not readily diffuse into the culture medium, but are released upon lysis of the cells.
A soluble factor produced by MONOCYTES; MACROPHAGES, and other cells which activates T-lymphocytes and potentiates their response to mitogens or antigens. Interleukin-1 is a general term refers to either of the two distinct proteins, INTERLEUKIN-1ALPHA and INTERLEUKIN-1BETA. The biological effects of IL-1 include the ability to replace macrophage requirements for T-cell activation.
The property of blood capillary ENDOTHELIUM that allows for the selective exchange of substances between the blood and surrounding tissues and through membranous barriers such as the BLOOD-AIR BARRIER; BLOOD-AQUEOUS BARRIER; BLOOD-BRAIN BARRIER; BLOOD-NERVE BARRIER; BLOOD-RETINAL BARRIER; and BLOOD-TESTIS BARRIER. Small lipid-soluble molecules such as carbon dioxide and oxygen move freely by diffusion. Water and water-soluble molecules cannot pass through the endothelial walls and are dependent on microscopic pores. These pores show narrow areas (TIGHT JUNCTIONS) which may limit large molecule movement.
A cytotoxic member of the CYTOCHALASINS.
An integrin alpha subunit that is unique in that it does not contain an I domain, and its proteolytic cleavage site is near the middle of the extracellular portion of the polypeptide rather than close to the membrane as in other integrin alpha subunits.
Phenomenon of cell-mediated immunity measured by in vitro inhibition of the migration or phagocytosis of antigen-stimulated LEUKOCYTES or MACROPHAGES. Specific CELL MIGRATION ASSAYS have been developed to estimate levels of migration inhibitory factors, immune reactivity against tumor-associated antigens, and immunosuppressive effects of infectious microorganisms.
An iron-binding protein that was originally characterized as a milk protein. It is widely distributed in secretory fluids and is found in the neutrophilic granules of LEUKOCYTES. The N-terminal part of lactoferrin possesses a serine protease which functions to inactivate the TYPE III SECRETION SYSTEM used by bacteria to export virulence proteins for host cell invasion.
Trihydroxy derivatives of eicosanoic acids. They are primarily derived from arachidonic acid, however eicosapentaenoic acid derivatives also exist. Many of them are naturally occurring mediators of immune regulation.
The lipid- and protein-containing, selectively permeable membrane that surrounds the cytoplasm in prokaryotic and eukaryotic cells.
The endogenous compounds that mediate inflammation (AUTACOIDS) and related exogenous compounds including the synthetic prostaglandins (PROSTAGLANDINS, SYNTHETIC).
Serum glycoproteins participating in the host defense mechanism of COMPLEMENT ACTIVATION that creates the COMPLEMENT MEMBRANE ATTACK COMPLEX. Included are glycoproteins in the various pathways of complement activation (CLASSICAL COMPLEMENT PATHWAY; ALTERNATIVE COMPLEMENT PATHWAY; and LECTIN COMPLEMENT PATHWAY).
Cell adhesion molecules present on virtually all monocytes, platelets, and granulocytes. CD31 is highly expressed on endothelial cells and concentrated at the junctions between them.
The passage of cells across the layer of ENDOTHELIAL CELLS, i.e., the ENDOTHELIUM; or across the layer of EPITHELIAL CELLS, i.e. the EPITHELIUM.
Domesticated bovine animals of the genus Bos, usually kept on a farm or ranch and used for the production of meat or dairy products or for heavy labor.
The outer covering of the body that protects it from the environment. It is composed of the DERMIS and the EPIDERMIS.
An ionophorous, polyether antibiotic from Streptomyces chartreusensis. It binds and transports CALCIUM and other divalent cations across membranes and uncouples oxidative phosphorylation while inhibiting ATPase of rat liver mitochondria. The substance is used mostly as a biochemical tool to study the role of divalent cations in various biological systems.
Group of chemokines with paired cysteines separated by a different amino acid. CXC chemokines are chemoattractants for neutrophils but not monocytes.
A common name used for the genus Cavia. The most common species is Cavia porcellus which is the domesticated guinea pig used for pets and biomedical research.
A CD antigen that contains a conserved I domain which is involved in ligand binding. When combined with CD18 the two subunits form MACROPHAGE-1 ANTIGEN.
A layer of epithelium that lines the heart, blood vessels (ENDOTHELIUM, VASCULAR), lymph vessels (ENDOTHELIUM, LYMPHATIC), and the serous cavities of the body.
Histochemical localization of immunoreactive substances using labeled antibodies as reagents.
The intracellular transfer of information (biological activation/inhibition) through a signal pathway. In each signal transduction system, an activation/inhibition signal from a biologically active molecule (hormone, neurotransmitter) is mediated via the coupling of a receptor/enzyme to a second messenger system or to an ion channel. Signal transduction plays an important role in activating cellular functions, cell differentiation, and cell proliferation. Examples of signal transduction systems are the GAMMA-AMINOBUTYRIC ACID-postsynaptic receptor-calcium ion channel system, the receptor-mediated T-cell activation pathway, and the receptor-mediated activation of phospholipases. Those coupled to membrane depolarization or intracellular release of calcium include the receptor-mediated activation of cytotoxic functions in granulocytes and the synaptic potentiation of protein kinase activation. Some signal transduction pathways may be part of larger signal transduction pathways; for example, protein kinase activation is part of the platelet activation signal pathway.
Any of the processes by which nuclear, cytoplasmic, or intercellular factors influence the differential control (induction or repression) of gene action at the level of transcription or translation.
Test for cell-mediated antitumor immunity and related serum blocking factors based on the finding that leukocytes from cancer patients, but not from controls, when mixed in vitro with antigenic extracts of tumors of the same histological type, undergo a diminution in their normal adherence to glass surfaces. Sera from tumor-bearing patients block the LAI reaction of their own leukocytes or those of other patients with the same type of tumor.
A variation of the PCR technique in which cDNA is made from RNA via reverse transcription. The resultant cDNA is then amplified using standard PCR protocols.
Family of proteins associated with the capacity of LEUKOCYTES to adhere to each other and to certain substrata, e.g., the C3bi component of complement. Members of this family are the LYMPHOCYTE FUNCTION-ASSOCIATED ANTIGEN-1; (LFA-1), the MACROPHAGE-1 ANTIGEN; (Mac-1), and the INTEGRIN ALPHAXBETA2 or p150,95 leukocyte adhesion protein. They all share a common beta-subunit which is the CD18 antigen. All three of the above antigens are absent in inherited LEUKOCYTE-ADHESION DEFICIENCY SYNDROME, which is characterized by recurrent bacterial infections, impaired pus formation, and wound healing as well as abnormalities in a wide spectrum of adherence-dependent functions of granulocytes, monocytes, and lymphoid cells.
Morphologic alteration of small B LYMPHOCYTES or T LYMPHOCYTES in culture into large blast-like cells able to synthesize DNA and RNA and to divide mitotically. It is induced by INTERLEUKINS; MITOGENS such as PHYTOHEMAGGLUTININS, and by specific ANTIGENS. It may also occur in vivo as in GRAFT REJECTION.
The phenomenon by which dissociated cells intermixed in vitro tend to group themselves with cells of their own type.
Proteins prepared by recombinant DNA technology.
High-molecular weight glycoproteins uniquely expressed on the surface of LEUKOCYTES and their hemopoietic progenitors. They contain a cytoplasmic protein tyrosine phosphatase activity which plays a role in intracellular signaling from the CELL SURFACE RECEPTORS. The CD45 antigens occur as multiple isoforms that result from alternative mRNA splicing and differential usage of three exons.
The relationship between the dose of an administered drug and the response of the organism to the drug.
A cationic cytochemical stain specific for cell nuclei, especially DNA. It is used as a supravital stain and in fluorescence cytochemistry. It may cause mutations in microorganisms.
The space enclosed by the peritoneum. It is divided into two portions, the greater sac and the lesser sac or omental bursa, which lies behind the STOMACH. The two sacs are connected by the foramen of Winslow, or epiploic foramen.
A species of gram-negative, facultatively anaerobic, rod-shaped bacteria normally commensal in the flora of CATTLE and SHEEP. But under conditions of physical or PHYSIOLOGICAL STRESS, it can cause MASTITIS in sheep and SHIPPING FEVER or ENZOOTIC CALF PNEUMONIA in cattle. Its former name was Pasteurella haemolytica.
A gamma-emitting RADIONUCLIDE IMAGING agent used in the evaluation of regional cerebral blood flow and in non-invasive dynamic biodistribution studies and MYOCARDIAL PERFUSION IMAGING. It has also been used to label leukocytes in the investigation of INFLAMMATORY BOWEL DISEASES.
Serum that contains antibodies. It is obtained from an animal that has been immunized either by ANTIGEN injection or infection with microorganisms containing the antigen.
A CXC chemokine with specificity for CXCR2 RECEPTORS. It has growth factor activities and is implicated as a oncogenic factor in several tumor types.
The phenotypic manifestation of a gene or genes by the processes of GENETIC TRANSCRIPTION and GENETIC TRANSLATION.
Cells contained in the bone marrow including fat cells (see ADIPOCYTES); STROMAL CELLS; MEGAKARYOCYTES; and the immediate precursors of most blood cells.
The soft tissue filling the cavities of bones. Bone marrow exists in two types, yellow and red. Yellow marrow is found in the large cavities of large bones and consists mostly of fat cells and a few primitive blood cells. Red marrow is a hematopoietic tissue and is the site of production of erythrocytes and granular leukocytes. Bone marrow is made up of a framework of connective tissue containing branching fibers with the frame being filled with marrow cells.
Accumulation of purulent material in tissues, organs, or circumscribed spaces, usually associated with signs of infection.
Surface antigens expressed on myeloid cells of the granulocyte-monocyte-histiocyte series during differentiation. Analysis of their reactivity in normal and malignant myelomonocytic cells is useful in identifying and classifying human leukemias and lymphomas.
The number of CELLS of a specific kind, usually measured per unit volume or area of sample.
Molecules on the surface of some B-lymphocytes and macrophages, that recognize and combine with the C3b, C3d, C1q, and C4b components of complement.
A trisaccharide antigen expressed on glycolipids and many cell-surface glycoproteins. In the blood the antigen is found on the surface of NEUTROPHILS; EOSINOPHILS; and MONOCYTES. In addition, CD15 antigen is a stage-specific embryonic antigen.
Cell surface proteins that bind signalling molecules external to the cell with high affinity and convert this extracellular event into one or more intracellular signals that alter the behavior of the target cell (From Alberts, Molecular Biology of the Cell, 2nd ed, pp693-5). Cell surface receptors, unlike enzymes, do not chemically alter their ligands.
A positive regulatory effect on physiological processes at the molecular, cellular, or systemic level. At the molecular level, the major regulatory sites include membrane receptors, genes (GENE EXPRESSION REGULATION), mRNAs (RNA, MESSENGER), and proteins.
Protein of the annexin family exhibiting lipid interaction and steroid-inducibility.
A milky, product excreted from the latex canals of a variety of plant species that contain cauotchouc. Latex is composed of 25-35% caoutchouc, 60-75% water, 2% protein, 2% resin, 1.5% sugar & 1% ash. RUBBER is made by the removal of water from latex.(From Concise Encyclopedia Biochemistry and Molecular Biology, 3rd ed). Hevein proteins are responsible for LATEX HYPERSENSITIVITY. Latexes are used as inert vehicles to carry antibodies or antigens in LATEX FIXATION TESTS.
Adverse functional, metabolic, or structural changes in ischemic tissues resulting from the restoration of blood flow to the tissue (REPERFUSION), including swelling; HEMORRHAGE; NECROSIS; and damage from FREE RADICALS. The most common instance is MYOCARDIAL REPERFUSION INJURY.
The capacity of a normal organism to remain unaffected by microorganisms and their toxins. It results from the presence of naturally occurring ANTI-INFECTIVE AGENTS, constitutional factors such as BODY TEMPERATURE and immediate acting immune cells such as NATURAL KILLER CELLS.
A species of gram-negative, facultatively anaerobic, rod-shaped bacteria (GRAM-NEGATIVE FACULTATIVELY ANAEROBIC RODS) commonly found in the lower part of the intestine of warm-blooded animals. It is usually nonpathogenic, but some strains are known to produce DIARRHEA and pyogenic infections. Pathogenic strains (virotypes) are classified by their specific pathogenic mechanisms such as toxins (ENTEROTOXIGENIC ESCHERICHIA COLI), etc.
A major alkaloid from Colchicum autumnale L. and found also in other Colchicum species. Its primary therapeutic use is in the treatment of gout, but it has been used also in the therapy of familial Mediterranean fever (PERIODIC DISEASE).
Tetraspanin proteins found at high levels in cells of the lymphoid-myeloid lineage. CD53 antigens may be involved regulating the differentiation of T-LYMPHOCYTES and the activation of B-LYMPHOCYTES.
Integrin alpha4beta1 is a FIBRONECTIN and VCAM-1 receptor present on LYMPHOCYTES; MONOCYTES; EOSINOPHILS; NK CELLS and thymocytes. It is involved in both cell-cell and cell- EXTRACELLULAR MATRIX adhesion and plays a role in INFLAMMATION, hematopoietic cell homing and immune function, and has been implicated in skeletal MYOGENESIS; NEURAL CREST migration and proliferation, lymphocyte maturation and morphogenesis of the PLACENTA and HEART.
The major interferon produced by mitogenically or antigenically stimulated LYMPHOCYTES. It is structurally different from TYPE I INTERFERON and its major activity is immunoregulation. It has been implicated in the expression of CLASS II HISTOCOMPATIBILITY ANTIGENS in cells that do not normally produce them, leading to AUTOIMMUNE DISEASES.
Granular leukocytes characterized by a relatively pale-staining, lobate nucleus and cytoplasm containing coarse dark-staining granules of variable size and stainable by basic dyes.
Bone marrow-derived lymphocytes that possess cytotoxic properties, classically directed against transformed and virus-infected cells. Unlike T CELLS; and B CELLS; NK CELLS are not antigen specific. The cytotoxicity of natural killer cells is determined by the collective signaling of an array of inhibitory and stimulatory CELL SURFACE RECEPTORS. A subset of T-LYMPHOCYTES referred to as NATURAL KILLER T CELLS shares some of the properties of this cell type.
Abnormal intravascular leukocyte aggregation and clumping often seen in leukemia patients. The brain and lungs are the two most commonly affected organs. This acute syndrome requires aggressive cytoreductive modalities including chemotherapy and/or leukophoresis. It is differentiated from LEUKEMIC INFILTRATION which is a neoplastic process where leukemic cells invade organs.
Infection with CYTOMEGALOVIRUS, characterized by enlarged cells bearing intranuclear inclusions. Infection may be in almost any organ, but the salivary glands are the most common site in children, as are the lungs in adults.
Potentially pathogenic bacteria found in nasal membranes, skin, hair follicles, and perineum of warm-blooded animals. They may cause a wide range of infections and intoxications.
The 8-hydroxy derivatives inhibit various enzymes and their halogenated derivatives, though neurotoxic, are used as topical anti-infective agents, among other uses.
An unsaturated, essential fatty acid. It is found in animal and human fat as well as in the liver, brain, and glandular organs, and is a constituent of animal phosphatides. It is formed by the synthesis from dietary linoleic acid and is a precursor in the biosynthesis of prostaglandins, thromboxanes, and leukotrienes.
A sialic acid-rich protein and an integral cell membrane mucin. It plays an important role in activation of T-LYMPHOCYTES.
Family of antimicrobial peptides that have been identified in humans, animals, and plants. They are thought to play a role in host defenses against infections, inflammation, wound repair, and acquired immunity.
An enzyme of the oxidoreductase class primarily found in PLANTS. It catalyzes reactions between linoleate and other fatty acids and oxygen to form hydroperoxy-fatty acid derivatives.
A CC-type chemokine that is a chemoattractant for EOSINOPHILS; MONOCYTES; and LYMPHOCYTES. It is a potent and selective eosinophil chemotaxin that is stored in and released from PLATELETS and activated T-LYMPHOCYTES. Chemokine CCL5 is specific for CCR1 RECEPTORS; CCR3 RECEPTORS; and CCR5 RECEPTORS. The acronym RANTES refers to Regulated on Activation, Normal T Expressed and Secreted.
INFLAMMATION of PLEURA, the lining of the LUNG. When PARIETAL PLEURA is involved, there is pleuritic CHEST PAIN.
A family of transmembrane glycoproteins (MEMBRANE GLYCOPROTEINS) consisting of noncovalent heterodimers. They interact with a wide variety of ligands including EXTRACELLULAR MATRIX PROTEINS; COMPLEMENT, and other cells, while their intracellular domains interact with the CYTOSKELETON. The integrins consist of at least three identified families: the cytoadhesin receptors(RECEPTORS, CYTOADHESIN), the leukocyte adhesion receptors (RECEPTORS, LEUKOCYTE ADHESION), and the VERY LATE ANTIGEN RECEPTORS. Each family contains a common beta-subunit (INTEGRIN BETA CHAINS) combined with one or more distinct alpha-subunits (INTEGRIN ALPHA CHAINS). These receptors participate in cell-matrix and cell-cell adhesion in many physiologically important processes, including embryological development; HEMOSTASIS; THROMBOSIS; WOUND HEALING; immune and nonimmune defense mechanisms; and oncogenic transformation.
An immunoassay utilizing an antibody labeled with an enzyme marker such as horseradish peroxidase. While either the enzyme or the antibody is bound to an immunosorbent substrate, they both retain their biologic activity; the change in enzyme activity as a result of the enzyme-antibody-antigen reaction is proportional to the concentration of the antigen and can be measured spectrophotometrically or with the naked eye. Many variations of the method have been developed.
A class of cell surface leukotriene receptors with a preference for leukotriene B4. Leukotriene B4 receptor activation influences chemotaxis, chemokinesis, adherence, enzyme release, oxidative bursts, and degranulation in polymorphonuclear leukocytes. There are at least two subtypes of these receptors. Some actions are mediated through the inositol phosphate and diacylglycerol second messenger systems.

Phagocytic acitivity of bovine leukocytes during pregnancy. (1/9769)

The phagocytic competence, measured as the total number of polymorphonuclear leukocytes per mm3 which phagocytosed Staphylococcus aureus, strain 321, in vitro, was determined in eight cows during complete pregnancies. Such leukocytes are referred to as "Active PMN'S". There was a gradual decline in the number of these cells from conception to a minimum between the 16th and 20th weeks of pregnancy, followed by a steady increase to the cessation of lactation when a marked drop occurred, after which there was an increase to a maximun during the second week prepartum. From this maximum there was a rapid decrease to an absolute minimum during the first week after parturition. From the second week postpartum there was a gradual increase to conception. The correlation coefficient (r) of number of active PMN'S with time before conception was -0.474 )p-0.01). There were significant differences (p=0.01) in numbers of active PMNS Among the eight cows. It was found that the cows fell into two groups, one whose members had, overall, significantly more active PMNs (p=0.001) than those in the second group. The between cow differences may have been due to 1) age, since the cows with the highest numbers of circulating active PMNs were younger than those in the other group of 2) the combined stress of pregnancy and lactation, as those cows which were both pregnant and milking had the lowest numbers of active PMNs.  (+info)

Blocking very late antigen-4 integrin decreases leukocyte entry and fatty streak formation in mice fed an atherogenic diet. (2/9769)

Atherosclerotic lesion development is characterized by the recruitment of leukocytes, principally monocytes, to the vessel wall. Considerable interest has been focused on the adhesion molecule(s) involved in leukocyte/endothelial interactions. The goal of the present study was to determine the role of the very late antigen-4 (VLA-4) integrin/ligand interaction in fatty streak development using murine models. Because alpha4 null mice are not viable, a peptidomimetic was used to block VLA-4-mediated leukocyte binding. The ability of a synthetic peptidomimetic of connecting segment-1 (CS-1 peptide) to block the recruitment of leukocytes and the accumulation of lipid in the aortic sinus of either wild-type mice (strain C57BL/6J) or mice with a low-density lipoprotein null mutation (LDLR-/-) maintained on an atherogenic diet was assessed. The active (Ac) CS-1 peptide or scrambled (Sc) CS-1 peptide was delivered subcutaneously into mice using a mini osmotic pump. Mice were exposed to the peptide for 24 to 36 hours before the onset of the atherogenic diet. In C57BL/6J mice, leukocyte entry into the aortic sinus, as assessed by en face preparations, was inhibited by the active peptide (Ac=28+/-4, Sc=54+/-6 monocytes/valve; P=0.004). Additionally, frozen sections stained with Oil Red O were analyzed to assess lipid accumulation in the aortic sinus. C57BL/6J mice that received the (Ac) compound demonstrated significantly reduced lesion areas as compared with mice that received the (Sc) peptide (Ac=4887+/-4438 microm2, Sc=15 009 +/-5619 microm2; P<0.0001). In a separate study, LDLR-/- mice were implanted with pumps containing either the (Ac) or (Sc) peptide before initiation of the atherogenic diet. Because LDLR-/- mice fed a chow diet displayed small lesions at 14 weeks, the effects of the peptide seen in these animals represented a change in early lipid accumulation rather than initiation. By using whole-mount preparations, the (Ac) but not the (Sc) peptide significantly reduced the area of lipid accumulation in the aortic sinus, resulting in an approximate 66% decrease. Plasma analysis from all studies revealed concentrations of peptide to be present at levels previously determined by in vitro analysis to block adhesion. (Ac) CS-1 peptide, which blocks VLA-4 on the leukocyte surface, is effective in reducing leukocyte recruitment and lipid accumulation in the aortic sinus. The present study provides in vivo evidence that the VLA-4 integrin plays an important role in the initiation of the atherosclerotic lesion and lipid accumulation, and it suggests a potential therapeutic strategy for this disease.  (+info)

Inhibition of L-selectin-mediated leukocyte rolling by synthetic glycoprotein mimics. (3/9769)

Synthetic carbohydrate and glycoprotein mimics displaying sulfated saccharide residues have been assayed for their L-selectin inhibitory properties under static and flow conditions. Polymers displaying the L-selectin recognition epitopes 3',6-disulfo Lewis x(Glc) (3-O-SO3-Galbeta1alpha4(Fucalpha1alpha3)-6-O-SO3-Glcbeta+ ++-OR) and 3',6'-disulfo Lewis x(Glc) (3, 6-di-O-SO3-Galbeta1alpha4(Fucalpha1alpha3)Glcbeta-OR) both inhibit L-selectin binding to heparin under static, cell-free binding conditions with similar efficacies. Under conditions of shear flow, however, only the polymer displaying 3',6-disulfo Lewis x(Glc) inhibits the rolling of L-selectin-transfected cells on the glycoprotein ligand GlyCAM-1. Although it has been shown to more effective than sialyl Lewis x at blocking the L-selectin-GlyCAM-1 interaction in static binding studies, the corresponding monomer had no effect in the dynamic assay. These data indicate that multivalent ligands are far more effective inhibitors of L-selectin-mediated rolling than their monovalent counterparts and that the inhibitory activities are dependent on the specific sulfation pattern of the recognition epitope. Importantly, our results indicate the L-selectin specificity for one ligand over another found in static, cell-free binding assays is not necessarily retained under the conditions of shear flow. The results suggest that monovalent or polyvalent carbohydrate or glycoprotein mimetics that inhibit selectin binding in static assays may not block the more physiologically relevant process of selectin-mediated rolling.  (+info)

Changes in haematological parameters and iron metabolism associated with a 1600 kilometre ultramarathon. (4/9769)

OBJECTIVE: To investigate haematological variations and iron related changes in the serum of participants in a 1600 kilometre ultramarathon run. PARTICIPANTS: Seven male and two female participants in a 1600 km foot race. METHODS: Blood samples were obtained from the participants before, after four and 11 days of running, and at the end of the event. Samples were analysed by standard methods for haemoglobin, packed cell volume, total red cell count, mean red cell volume, mean red cell haemoglobin, total white cell count and differential, platelets, reticulocytes, iron, ferritin, total iron binding capacity, percentage transferrin saturation, haptoglobin, and bilirubin and corrected for changes in plasma volume. RESULTS: The following variables decreased during the event (p < 0.05): haemoglobin, packed cell volume, mean red cell volume, percentage lymphocytes, percentage monocytes, serum iron, total iron binding capacity, and percentage transferrin saturation. Increases (p < 0.05) were found in plasma volume, total red cell count (day 4 only), total white cell count, percentage and absolute numbers of neutrophils and reticulocytes, absolute numbers of lymphocytes and monocytes (day 4 only), absolute numbers of eosinophils (day 11 and race end), absolute numbers of basophils (race end only), platelets, ferritin, haptoglobin, and bilirubin (day 4 only). CONCLUSION: Ultramarathon running is associated with a wide range of changes in haematological parameters, many of which are related to the normal acute phase response to injury. These should not be confused with indicators of disease.  (+info)

Phenotypic and functional studies of leukocytes in human endometrium and endometriosis. (5/9769)

The aetiology of endometriosis, a common and disabling disorder, is presently unknown, although immune dysfunction could allow ectopic endometrial fragments to survive outside the uterine cavity. These studies investigate the relationship between leukocyte populations, steroid hormone receptor expression, proliferative activity, bcl-2 expression and apoptosis in eutopic and ectopic endometrium from women with endometriosis or adenomyosis at different phases of the menstrual cycle. Significantly increased oestrogen receptor expression, bcl-2 expression and numbers of CD8+ leukocytes were found in ectopic compared with eutopic endometrium in endometriosis, and CD56+ endometrial granulated lymphocytes (eGLs) were significantly reduced in ectopic endometrium. Apoptotic cells were rarely found in control and subject endometria. In contrast with endometriosis, adenomyotic lesions showed identical steroid hormone receptor expression, proliferative activity, bcl-2 expression and leukocyte subpopulations to eutopic endometrium, indicating different aetiologies for these disorders. The unusual CD56+ CD16- eGLs present in large numbers in late secretory phase eutopic endometrium were highly purified (>98%) by immunomagnetic separation. Except for a negligible cytotoxic activity of eGLs from early proliferative samples, cytotoxic activity of eGLs from non-pregnant endometrium during the menstrual cycle was comparable with those in peripheral blood, predominantly CD56+ CD16+ natural killer cells. eGLs from non-pregnant endometrium and early pregnancy showed a variable proliferative response to 5 and 100 U/ml interleukin-2 over 48-h and 120-h time courses. eGLs are evidently functionally important in the eutopic endometrium. Their absence in endometriotic lesions together with increased CD+8 T-cell numbers and increased oestrogen receptor and bcl-2 expression may have significant effects on the development and progression of endometriosis.  (+info)

Differential expression and phosphorylation of CTCF, a c-myc transcriptional regulator, during differentiation of human myeloid cells. (6/9769)

CTCF is a transcriptional repressor of the c-myc gene. Although CTCF has been characterized in some detail, there is very little information about the regulation of CTCF activity. Therefore we investigated CTCF expression and phosphorylation during induced differentiation of human myeloid leukemia cells. We found that: (i) both CTCF mRNA and protein are down-regulated during terminal differentiation in most cell lines tested; (ii) CTCF down-regulation is retarded and less pronounced than that of c-myc; (iii) CTCF protein is differentially phosphorylated and the phosphorylation profiles depend on the differentiation pathway. We concluded that CTCF expression and activity is controlled at transcriptional and post-transcriptional levels.  (+info)

Identification of a novel activation-inducible protein of the tumor necrosis factor receptor superfamily and its ligand. (7/9769)

Among members of the tumor necrosis factor receptor (TNFR) superfamily, 4-1BB, CD27, and glucocorticoid-induced tumor necrosis factor receptor family-related gene (GITR) share a striking homology in the cytoplasmic domain. Here we report the identification of a new member, activation-inducible TNFR family member (AITR), which belongs to this subfamily, and its ligand. The receptor is expressed in lymph node and peripheral blood leukocytes, and its expression is up-regulated in human peripheral mononuclear cells mainly after stimulation with anti-CD3/CD28 monoclonal antibodies or phorbol 12-myristate 13-acetate/ionomycin. AITR associates with TRAF1 (TNF receptor-associated factor 1), TRAF2, and TRAF3, and induces nuclear factor (NF)-kappaB activation via TRAF2. The ligand for AITR (AITRL) was found to be an undescribed member of the TNF family, which is expressed in endothelial cells. Thus, AITR and AITRL seem to be important for interactions between activated T lymphocytes and endothelial cells.  (+info)

A sialoglycoprotein, gp20, of the human capacitated sperm surface is a homologue of the leukocyte CD52 antigen: analysis of the effect of anti-CD52 monoclonal antibody (CAMPATH-1) on capacitated spermatozoa. (8/9769)

In this study we performed N-terminal sequence analysis of gp20, a 20 kDa sialoglycoprotein on the human sperm surface previously identified by radiolabelling of the sialic acid residues of sperm surface. We found 100% identity with the N-terminus of CD52, an antigen expressed on almost all human leukocytes. We also show that, like CD52, gp20 behaves as a glycosylphosphatidylinositol (GPI)-anchored protein and that anti-gp20 antiserum reacts with an antigen on leukocytes of the same molecular weight as CD52. Using CAMPATH-1, the monoclonal antibody against CD52, in fluorescent staining of capacitated spermatozoa, Western blot analysis and the zona-free hamster egg penetration test, we found that the effect of this antibody was different from that of our anti-gp20. Western blot analysis revealed a well-defined 20 kDa band with anti-gp20, whereas a 14-20 kDa band was detected with CAMPATH-1. Anti-gp20 stained the equatorial region of the sperm head, whereas CAMPATH-1 stained the tail in immunofluorescence analysis of capacitated spermatozoa. A dose-dependent inhibitory effect was seen with CAMPATH-1, similar to that previously detected with anti-gp20, in a zona-free hamster egg penetration test. However, with CAMPATH-1 agglutination of motile spermatozoa was detected, and this was not present with anti-gp20. This suggests that the epitopes recognized by the two antibodies are different.  (+info)

Definition of Leukocyte-endothelial cell adhesion molecule 1 in the Legal Dictionary - by Free online English dictionary and encyclopedia. What is Leukocyte-endothelial cell adhesion molecule 1? Meaning of Leukocyte-endothelial cell adhesion molecule 1 as a legal term. What does Leukocyte-endothelial cell adhesion molecule 1 mean in law?
, Human Peripheral Blood Leukocyte (Normal) tissue lysate, GTX27767, Applications: ELISA, IP, WB; ELISA, Immunoprecipitation, Western Blot (WB); CrossReactivity:
What does HPBL stand for? Hop on to get the meaning of HPBL. The Acronym /Abbreviation/Slang HPBL means human peripheral blood leukocyte. by AcronymAndSlang.com
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The regulation of CD11b integrin levels on human blood leukocytes and leukotriene B4-stimulated skin by a specific leukotriene B4 receptor antagonist (LY293111 ...
Pathogenic bacteria produce numerous virulence factors, including protein toxins, to enhance their growth and survival within the host. Our lab seeks to understand the mechanisms of bacterial toxin delivery to identify and exploit therapeutic targets. In this project, we have focused on a member of the repeats-in-toxin (RTX) family of proteins, which are secreted by many Gram negative bacteria, including Bordetella pertussis, Escherichia coli, and Aggregatibacter actinomycetemcomitans. The leukotoxin (LtxA) secreted by A. actinomycetemcomitans specifically kills human white blood cells to disrupt the host immune response and therefore plays a key role in bacterial colonization of the host. The cell type specificity of LtxA arises from its reported targeting of the lymphocyte function-associated antigen-1 (LFA-1) integrin, which is only expressed by human white blood cells. In addition, we recently showed that LtxA binds with a strong affinity to cholesterol, and inhibition of this binding to ...
Background Huntingtons disease (HD) is caused by expanded CAG repeats encoding a polyglutamine tract in the huntingtin (HTT) protein. A number of differentially-expressed protein molecules have been identified in striatum of HD animal models. Here we examined if the expression changes could be visualized in the peripheral leukocytes of HD patients and pre-symptomatic HD (PreHD) carriers. Methods and findings The expression levels of 17 candidate genes that differentially expressed in striatum between transgenic HD and wild-type mice in literature were measured in the peripheral leukocytes of 4 PreHD carriers, 16 HD patients and 20 healthy controls. Four genes majorly involved in metabolism and oxidative stress response, including AHCY1, ACO2, OXCT1 and CAP1, demonstrated consistent downregulation in peripheral leukocytes of both PreHD carriers and HD patients, while UCP2 was only down-regulated in HD patients. Conclusion These results provide potential peripheral biomarkers to indicate disease
Cardiovascular events are the leading cause of death in developed countries worldwide, including Taiwan. Type 2 diabetes mellitus (T2DM), previously considered merely as one of the risk factors, has been recently unanimously accepted to be coronary artery disease-equivalent. How T2DM may lead to accelerated atherosclerosis remains obscure.. Hyperglycemia with or without hyperinsulinemia may lead to higher oxidative stress and generalized inflammation. The oxidative stress and inflammation may play a significant role in the pathogenesis in diabetic complications, including micro- and macro-vascular complications. Macrophages together with T-lymphocytes are the earliest cell-types found in fatty-streaks, the earliest atherosclerotic lesions. Macrophages are also well known cellular mediators of oxidative stress and inflammation. Therefore, it is plausible to hypothesize that macrophages play a crucial role in the pathogenesis of atherosclerosis in patients with T2DM. In addition, the other cell ...
Neutrophils represent the most abundant type of granulocytes, accounting for 25-75% of all circulating human white blood cells (PMID: 23435331, 22491176). Their mechanisms of action encompass degranulation of antimicrobial proteins, release of chemotactic factors (PMID: 22392929, 20140197, 25512469, 21555529), and formation of neutrophil extracellular traps or NETs (PMID: 23435331, 22491176, 15001782) in response to pathogens. Typical markers are CD15, CD66b, CD16, and the absence of CD49d.. Representing 1-10% of peripheral leukocytes, eosinophils in resting state reside mainly in the periphery, especially in lamina propria (PMID: 11877470, 25049430). They readily release their granule content including eosinophil cationic protein (ECP), eosinophil peroxidase (EPX), and major basic protein (MBP) as well as chemokines, and cytokines in response to pathogens (PMID: 25003763). They play a role in antigen presentation and T cell polarization (PMID: 25003763, 8450230, 20065995, 16551246). A specific ...
Apoptosis. Coloured scanning electron micrograph of human white blood cells (leucocytes), showing one cell undergoing apoptosis. Apoptosis is the process of genetically programmed cell death. At centre, an apoptotic white blood cell has shrunk and its cytoplasm has developed blebs (grape-like clusters). Normal white blood cells are seen beside it. These white blood cells are myeloid leucocytes, originating from bone marrow. The human myeloid cell line depends on growth factors to survive, and cells undergo apoptosis when deprived of growth factors. Research on apoptosis may provide genetic treatments for diseases such as cancer. Magnification: x1,500 at 6x7cm size. - Stock Image P248/0134
Non-healing wounds pose a major burden to patients and health care systems alike. These wounds are chronically stuck in the inflammatory phase of the healing process without transitioning to the proliferative phase. They are also characterized by the excessive presence of leukocytes which are assumed to provoke the persistent inflammation observed in pathological wound healing. Recent studies suggested a beneficial role of cold physical plasma in the treatment of chronic wounds. Hence, it was the central question, whether exposure to cold physical plasma would affect the viability and/or function of human leukocytes. Cold plasma displays various properties of which the generation of reactive molecules, such as reactive oxygen and nitrogen species (ROS/RNS), where found to be central in mediating redox changes in leukocytes. Oxidative stress was present especially in lymphocytes that readily underwent apoptosis after exposure to plasma. This was largely a direct consequence of plasma-generated hydrogen
Flow-FISH (fluorescent in-situ hybridization) is a cytogenetic technique to quantify the copy number of specific repetitive elements in genomic DNA of whole cell populations via the combination of flow cytometry with cytogenetic fluorescent in situ hybridization staining protocols. Flow-FISH is most commonly used to quantify the length of telomeres, which are stretches of repetitious DNA (hexameric TTAGGG repeats) at the distal ends of chromosomes in human white blood cells, and a semi-automated method for doing so was published in Nature Protocols. Telomere length in white blood cells has been a subject of interest because telomere length in these cell types (and also of other somatic tissues) declines gradually over the human lifespan, resulting in cell senescence, apoptosis, or transformation. This decline has been shown to be a surrogate marker for the concomitant decline in the telomere length of the hematopoietic stem cell pool, with the granulocyte lineage giving the best indication, ...
Positive evolutionary pressure has apparently preserved the ability to synthesize chemically authentic morphine, albeit in homeopathic concentrations, throughout animal phyla. ... The apparently serendipitous finding of an opiate alkaloid-sensitive, opioid peptide-insensitive, µ3 opiate receptor subtype expressed by invertebrate immunocytes, human blood monocytes, macrophage cell lines, and human blood granulocytes provided compelling validating evidence for an autonomous role of endogenous morphine as a biologically important cellular signalling molecule (Stefano et al., 1993; Cruciani et al., 1994; Stefano and Scharrer, 1994; Makman et al., 1995). ... Human white blood cells have the ability to make and release ...
Fingerprint Dive into the research topics of Receptor-operated, but not voltage-operated, calcium channels are involved in basophil leucocyte activation and histamine release. Together they form a unique fingerprint. ...
A study finds that fatigue severity is similar in prostate cancer patients receiving androgen deprivation therapy and CFS. The GABA - A receptor Diaze
colour enhanced TEM (transmission electron micrograph) of human white blood cells (polymorphonuclear leucocytes) provided with food in the form of the outer coates of yeast cells (zymosan, or yeast cell ghosts) which had foreign protein absorbed on them. The image shows the phagocytosis by the pmns of the yeast. Magnification: 20,000x. (Enhancement of 9c7000). - Stock Image P248/0329
The purpose of this study is to investigate whether low-intensity current affects cells in culture. Two types of human cells: white blood cells (lymphocytes) and leukemia cells (molt-4 cells), were studied. A low-intensity time-varying electric current (0.14 milliamp) generated by the Clark Zapper was applied to cell cultures via two platinum electrodes for 2 hrs at 37o C. Cell counts were made at different times after electric current application. Results show that the current had no significant effect on human white blood cells up to 24 hrs after exposure, whereas it significantly inhibited the growth of leukemia cells. At 24 hrs after exposure, concentration of leukemia cells exposed to the electric current was only 58% of that of non-exposed leukemia cells.. This data suggests that the electric current can selectively inhibit the growth of leukemia cells and does not significantly affect normal cells. A manuscript describing these results is in preparation for publication. Further research ...
The purpose of this study is to investigate whether low-intensity current affects cells in culture. Two types of human cells: white blood cells (lymphocytes) and leukaemia cells (molt-4 cells), were studied. A low-intensity time-varying electric current (0.14 milliamp) generated by the Clark Zapper was applied to cell cultures via two platinum electrodes for 2 hrs at 37o C. Cell counts were made at different times after electric current application. Results show that the current had no significant effect on human white blood cells up to 24 hrs after exposure, whereas it significantly inhibited the growth of leukaemia cells. At 24 hrs after exposure, concentration of leukaemia cells exposed to the electric current was only 58% of that of non-exposed leukaemia cells. These data suggest that the electric current can selectively inhibit the growth of leukaemia cells and does not significantly affect normal cells. A manuscript describing these results is in preparation for publication. In addition, ...
A new tissue culture model using human white blood cells shows how people with a latent -- or symptom-free -- tuberculosis infection are protected from active disease by a critical early step in their immune response, researchers say.
Diabetes is associated with oxidative stress and increased mortality, but a possible correlation between leukocyte-endothelium interactions, oxidative stress, and silent myocardial ischemia (SMI) is yet to be confirmed. Mitochondrial dysfunctio
For expression profiling to have a practical impact in the management of immune-related disease it is essential that it can be applied to peripheral blood cells. Early studies have used total peripheral blood mononuclear cells, and as a consequence the majority of the disease-related signatures identified have simply reflected differences in the relative abundance of individual cell types between patients and controls. To identify cell-specific changes in transcription it would be necessary to profile purified leucocyte subsets. We have used sequential rounds of positive selection to isolate CD4 and CD8 T cells, CD19 B cells, CD14 monocytes and CD16 neutrophils for microarray analysis from a single blood sample. We compared gene expression in cells isolated in parallel using either positive or negative selection and demonstrate that there are no significant consistent changes due to positive selection, and that the far inferior results obtained by negative selection are largely due to reduced purity.
Sethi, S., 2002: Inhibition of leukocyte-endothelial interactions by oxidized omega-3 fatty acids: a novel mechanism for the anti-inflammatory effects of omega-3 fatty acids in fish oil
In the past several years, scanning electron microscopy has become one of the popular methods in biology research. Two main technical advancements-improvement of specimen preparation and the improvement of the instrument-are responsible for the massive amount of data that has been generated in a relatively short time and have greatly widened our knowledge concerning a variety of surface features. However, scanning electron microscopic studies of the lymphocyte surface have provided the most excitement as well as generated many controversies. This book presents the current knowledge in this field and is a useful reference, especially for the beginners in this field. ...
Immune response after laser-photosensitiser application could be crucial in treatment of cancers, because without it there could be no systemic, long-term tumour control. Laser immunotherapy, a novel method for treatment of metastatic tumours, uses a near-infrared laser, a laser-absorbing dye indocy …
With this study, we have demonstrated that we can distinguish between individuals exposed and unexposed to tobacco smoke on the basis of mRNA expression in peripheral leukocytes. Furthermore, we have shown that active exposure to tobacco smoke is associated with a biologically relevant mRNA expression signature. Previously, several studies have shown that, experimentally, in vitro and in animals, single exposures [e.g., heat shock (6) and 17β-estradiol (7)] alter gene expression in peripheral leukocytes. Our study shows that the use of expression patterns can be expanded to identify a complex exposure in human observational studies.. In this study, we examined gene expression in total leukocytes, rather than specific cellular subsets. Total numbers of peripheral leukocytes characteristically have been shown to differ by smoking status (8, 9), and differential counts have been reported to differ by number of cigarettes smoked (8) or to remain unchanged (10). Recently, interindividual variation ...
The lymphatic system includes specialised organs and tissues where leukocytes develop. During an immune response to pathogens, we may become aware of swollen lymph nodes (popularly called glands) in the neck, armpits or groin, which enlarge when the leukocytes they contain are multiplying near a site of infection. Leukocytes can distinguish between self, the cells and proteins generated by the organism whose body they patrol, and non-self (or foreign) material such as pathogens that originated outside the hosts body. Leukocytes are self-tolerant, i.e. they do not normally attack the hosts own cells or body proteins, but direct their actions only against non-self material that may pose a threat.. Although we have referred to the immune response, as if it was just one thing, in fact, there are two types of immune response, distinguished as innate and adaptive immunity.. ...
A platelet/leukocyte interaction assay method and reagent therefor are provided using the presence of a solid-phase stimulus, such as magnetic or non-magnetic particles or mixtures thereof, having bound to the surface thereof one or more ligands that interact directly with platelets, leukocytes or both, for providing a fast, reliable point-of-care assessment of platelet/leukocyte interaction.
Immune cell therapy is considered one of the most promising anti-cancer strategy in many human cancers. Compared to the destructive methods such as surgery, radiation, and chemotherapy, anti-cancer immune therapy is safer and less toxic method in the treatment of human cancer patients.. Among the immune cell therapy, autologous adoptive immune cell therapy is a method to transfer the immune cells derived from peripheral white blood cells and expanded and stimulated with various cytokines and tumor specific antigens in cancer patients. Recent development of the technique to expand immune cells ex vivo make autologous adoptive immune cell therapy much more feasible and popular. However, immune cell therapy showed response of below 10% currently in several clinical trials. The reason of poor response is that the adopted immune cells have to overcome the highly immune compromised environment in advanced or recurrent cancer patients.. The low-dose radiation, defined as the radiation below the ...
Borochova K, Niespodziana K, Focke-Tejkl M, Hofer G, Keller W, Valenta R. Dissociation of the respiratory syncytial virus F protein-specific human IgG, IgA and IgM response (2021), Scientific Reports volume 11, 3551; https://www.nature.com/articles/s41598-021-82893-y. Kratzer B, Trapin D, Ettel P, Körmöczi U, Rottal A, Tuppy F, Feichter M, Gattinger P, Borochova K, Dorofeeva Y, Tulaeva I, Weber M, Grabmeier-Pfisterhammer K, Tauber P, Gerdov M, Mühl B, Perkmann T, Fae I, Wennda S, Führer H, Henning R, Valenta R, Pickl W. Immunological imprint of COVID-19 on human peripheral blood leukocyte populations (2020). Allergy, 2020;00:1-15; https://doi.org/10.1111/all.14647.. Gattinger P, Borochova K, Dorofeeva Y, Henning R, Kiss R, Kratzer B, Mühl B, Perkmann T, Trapin D, Trella M, Ettel P, Tulaeva I, Pickl W, Valenta R. Antibodies in serum of convalescent patients following mild COVID‐19 do not always prevent virus‐receptor binding (2020). Allergy, 2020;00:1-6; ...
BOZLU, Gulcin; TANRIVERDI, Huseyin; ASLAN, Gonul and KUYUCU, Necdet. The value of acute phase reactants and LightCycler® SeptiFast test in the diagnosis of bacterial and viral infections in pediatric patients. Arch. argent. pediatr. [online]. 2018, vol.116, n.1, pp.35-41. ISSN 0325-0075. http://dx.doi.org/10.5546/aap.2018.35.. Introduction: This study was performed to investigate the value of acute phase reactants and LightCycler® SeptiFast test to differentiate bacterial and viral infections. Population and methods: Children with fever were enrolled to this prospective study. Peripheral white blood cell (WBC), C-reactive protein (CRP) and procalcitonin (PCT) were studied from all patients on day 1, 3 and 7. Blood culture and chest X-ray were also obtained on day 1. Blood samples for LightCycler® SeptiFast test were obtained in all patients to use them if there was uncertain diagnosis between bacterial or viral infection. The patients were divided into two groups as bacterial and viral ...
Differential Gene Expression in Peripheral White Blood Cells with Permissive Underfeeding and Standard Feeding in Critically Ill Patients: A Descriptive Sub-study of the PermiT Randomized Controlled Trial ...
Effect of recombinant cytokines on leucocytes and physiological changes in bovine mammary glands during early involution.: We examined the effects of administer
Immune function is generally reported to be impaired in uncontrolled diabetes mellitus. We have previously reported that very-low-density lipoproteins (VLDL) from diabetic rat serum injure endothelial cells in vitro. Thus, we wanted to determine if VLDL would also injure lymphocytes and impair the immunity of the streptozotocin-induced diabetic rat. The immunologie functions of spleen cells from normal and diabetic rats were studied using the in vitro mitogenic stimulation assay and mixed lymphocyte responses (MLR). The effects of diabetic serum and VLDL isolated from diabetic rat serum on normal spleen cells were also determined. Both the absolute peripheral blood leukocyte (PBL) and spleen cell numbers of diabetic rats were significantly decreased from those of normal rats (P , 0.02). In PBL there was a decreased percentage of lymphocytes and increased percentage of neutrophils (both P , 0.02). The mitogenic stimulation responses of spleen cells from diabetic rats were consistently lower than ...
Leukocytes play an important role in the human immune system. The family of leukocytes is comprised of lymphocytes, monocytes, eosinophils, basophils, and neutrophils. Any infection or acute stress may increase or decrease the number of leukocytes. An increased percentage of neutrophils may be caused by an acute infection, while an increased percentage of lymphocytes can be caused by a chronic bacterial infection. It is important to realize an abnormal variation in the leukocytes. The five types of leukocytes can be distinguished by their cytoplasmic granules, staining properties of the granules, size of cell, the proportion of the nuclear to the cytoplasmic material, and the type of nucleolar lobes. The number of lobes increased when leukemia, chronic nephritis, liver disease, cancer, sepsis, and vitamin B12 or folate deficiency occurred. Clinical neutrophil hypersegmentation has been widely used as an indicator of B12 or folate deficiency.Biomedical technologists can currently recognize abnormal
Shop Leukocyte surface antigen ELISA Kit, Recombinant Protein and Leukocyte surface antigen Antibody at MyBioSource. Custom ELISA Kit, Recombinant Protein and Antibody are available.
Leukocytes, which mount antitumor immune responses, present a barrier and selective pressure in tumor progression (19). Innate immune responses do not rely on antigens for activation, represent the first-line of defense against pathogens and cancer, and are responsible for activating adaptive immunity. In normal breast, CD45+ leukocytes are relatively rare, but detectable in both stroma and within mammary ducts (20). In DCIS, leukocytes are abundant in the stroma surrounding the ducts (especially in high-grade and HER2+ lesions), but intraepithelial leukocytes are rarely detectable (21). Leukocytes also localize to sites of myoepithelial cell layer disruption/microinvasion (21). This limited interaction between leukocytes and cancer cells in DCIS may underlie a mechanism by which tumors evade immune surveillance. Therefore, in DCIS, tumors could still exist in the equilibrium phase, with immune escape likely occurring during or just prior to invasive transition (Fig. 1A).. Both, myeloid and ...
Leukocytes, which mount antitumor immune responses, present a barrier and selective pressure in tumor progression (19). Innate immune responses do not rely on antigens for activation, represent the first-line of defense against pathogens and cancer, and are responsible for activating adaptive immunity. In normal breast, CD45+ leukocytes are relatively rare, but detectable in both stroma and within mammary ducts (20). In DCIS, leukocytes are abundant in the stroma surrounding the ducts (especially in high-grade and HER2+ lesions), but intraepithelial leukocytes are rarely detectable (21). Leukocytes also localize to sites of myoepithelial cell layer disruption/microinvasion (21). This limited interaction between leukocytes and cancer cells in DCIS may underlie a mechanism by which tumors evade immune surveillance. Therefore, in DCIS, tumors could still exist in the equilibrium phase, with immune escape likely occurring during or just prior to invasive transition (Fig. 1A).. Both, myeloid and ...
Endothelial cells play an important role in the regulation of thrombosis. Normal resting (i.e. unstimulated) endothelial cells exhibit antithrombotic activity. This property is due to an active participation of endothelial cells in the inhibition of
Definition of migration of leukocytes in the Financial Dictionary - by Free online English dictionary and encyclopedia. What is migration of leukocytes? Meaning of migration of leukocytes as a finance term. What does migration of leukocytes mean in finance?
Article Low doses of γ-radiation induce consistent protein expression changes in human leukocytes. Twenty percent of cancer patients experience adverse effects after radiotherapy. The therapeutic doses are adjusted to the most sensitive individuals, ...
Human Leukocyte Membrane Tissue Lysate (Adult Membrane Normal). Tested Reactivity: Hu. Validated: WB, IP. Backed by our 100% Guarantee.
Leukocytes play an important role in the development and progression of various inflammatory diseases. Primary leukocytes are blood cells that patrol the body and possess a potent arsenal of bactericidal agents and chemical messengers that regulate inflammation, immune responses, blood vessel formation and wound healing.. Fildeltas Pharmacology group has developed a variety of assays on primary leukocytes as a first step of an in vitro cascade for the characterization of potential anti-inflammatory effects of new drugs. Our portfolio for leukocyte activation covers various types of immune responses with multiple readouts and endpoints. Most of the assays are highly flexible in terms of set up and adaptable. Applying experience and expertise, Fidelta offers customized assay development as need.. ...
Several mechanisms are associated with brain dysfunction during sepsis; one of the most important are activation of microglia and astrocytes. Activation of glial cells induces changes in permeability of the blood-brain barrier, secretion of inflammatory cytokines, and these alterations could induce neuronal dysfunction. Furthermore, blood-borne leukocytes can also reach the brain and participate in inflammatory response. Mechanisms involved in sepsis-associated brain dysfunction were revised here, focusing in neuroinflammation and involvement of blood-borne leukocytes and glial cells in this process.
Answer questions correctly. (1 A sexually producing organism has 12 chromosomes in each somatic cell, how many chromosomes would you find in the organisms gametes (sperm/egg)? (2) The # of chromosomes in the human white blood.
Background: Clinical characteristics evaluation and risk factors identification of the coronavirus disease 2019 (COVID-19) have been well studied, while effecti
Ulcerative colitis is a nonspecific inflammatory disorder characterised by oxidative and nitrosative stress, leucocyte infiltration and upregulation of
White blood cells or leukocytes are immune cells. High in infections, SLE, leukemia...Low in alcoholism, medications, AIDS, chemotherapy
RGS1 promotes leukocyte accumulation in the aortic wall during Ang II-induced vascular inflammation.Flow cytometric analysis of bead-labelled aortic leukocytes
Semantic Scholar extracted view of Immune responsiveness to Mycobacterium leprae of healthy humans. Application of the leucocyte migration inhibition test. by Bjørn Myrvang
We demonstrate an additional step and a positive feedback loop in leukocyte accumulation on inflamed endothelium. Leukocytes in shear flow bind to adherent leukocytes through L-selectin/ligand interactions and subsequently bind downstream and roll on inflamed endothelium, purified E-selectin, P-selectin, L-selectin, VCAM-1, or peripheral node addressin. Thus adherent leukocytes nucleate formation of strings of rolling cells and synergistically enhance leukocyte accumulation. Neutrophils, monocytes, and activated T cell lines, but not peripheral blood T lymphocytes, tether to each other through L-selectin. L-selectin is not involved in direct binding to either E- or P-selectin and is not a major counterreceptor of endothelial selectins. Leukocyte-leukocyte tethers are more tolerant to high shear than direct tethers to endothelial selectins and, like other L-selectin-mediated interactions, require a shear threshold. Synergism between leukocyte-leukocyte and leukocyte-endothelial interactions ...
Analysis of the effect of cyclophosphamide on peripheral blood leukocyte gene expression. Certain chemotherapeutic drugs such as cyclophosphamide can enhance the antitumor efficacy of immunotherapy because of their capacity to modulate innate and adaptive immunity. Indeed, it has been argued that this capacity may be more significant to chemotherapeutic efficacy in general than is presently appreciated. To gain insights into the core mechanisms of chemoimmunotherapy, we methodically profiled the effects of cyclophosphamide on gene expression in bone marrow, spleen and peripheral blood, and on cytokine expression in plasma and bone marrow of tumor-bearing mice. Gene and protein expression were modulated early and transiently by cyclophosphamide, leading to upregulation of a variety of immunomodulatory factors, including danger signals, pattern recognition receptors, inflammatory mediators, growth factors, cytokines, chemokines and chemokine receptors. These factors are involved in sensing
Interaction of leukocytes in flow with adherent leukocytes may contribute to their accumulation at sites of inflammation. Using L-selectin immobilized in a flow chamber, a model system that mimics presentation of L-selectin by adherent leukocytes, we characterize ligands for L-selectin on leukocytes and show that they mediate tethering and rolling in shear flow. We demonstrate the presence of L-selectin ligands on granulocytes, monocytes, and myeloid and lymphoid cell lines, and not on peripheral blood T lymphocytes. These ligands are calcium dependent, sensitive to protease and neuraminidase, and structurally distinct from previously described ligands for L-selectin on high endothelial venules (HEV). Differential sensitivity to O-sialo-glycoprotease provides evidence for ligand activity on both mucin-like and nonmucin-like structures. Transfection with fucosyltransferase induces expression of functional L-selectin ligands on both a lymphoid cell line and a nonhematopoietic cell line. L-selectin ...
Glutathione S-transferase activity was identified in cytosol from human lymphoid-cell lines and peripheral leucocytes (polymorphonuclear-leucocyte/monocyte and small-lymphocyte fractions) and compared with human liver enzyme. The findings of closely similar elution volume in gel filtration, substrate (1-chloro-2,4-dinitrobenzene) and inhibitory (probenecid) kinetics indicate that the liver, leucocyte and lymphoid-cell transferases are closely related. The interaction of reduced glutathione and 1-chloro-2,4-dinitrobenzene was shown to occur in intact-lymphoid-cell culture, to be linear with time and quantity of cells and to have kinetics similar to those of the enzyme reaction catalysed by cytosol. ...
Apoptosis or programmed cell death plays a critical role in both inflammatory and immune responses. Recent evidence demonstrates that control of leukocyte apoptosis is one of the most striking immune system-related roles of melatonin. For this reason, this study evaluated the protective effects of melatonin on human leukocyte apoptosis induced by sustained cytosolic calcium increases. Such protective effects are likely mediated by melatonins free-radical scavenging actions. Treatments with the specific inhibitor of cytosolic calcium re-uptake, thapsigargin (TG), and/or the calcium-mobilizing agonist, N-formyl-methionyl-leucyl-phenylalanine (FMLP), induced intracellular reactive oxygen species (ROS) production, caspase activation as well as DNA fragmentation in human leukocytes. Also, TG- and/or FMLP-induced apoptosis was dependent on both cytosolic calcium increases and calcium uptake into mitochondria, because when cells were preincubated with the cytosolic calcium chelator, dimethyl BAPTA, and the
Background: Bulky DNA adducts are markers of exposure to genotoxic aromatic compounds, which reflect the ability of an individual to metabolically activate carcinogens and to repair DNA damage. Polycyclic aromatic hydrocarbons (PAHs) represent a major class of carcinogens that are capable of forming such adducts. Factors that have been reported to be related to DNA adduct levels include smoking, diet, body mass index (BMI), genetic polymorphisms, the season of collection of biologic material, and air pollutants.. Methods: We pooled 11 studies (3,600 subjects) in which bulky DNA adducts were measured in human white blood cells with similar 32P-postlabeling techniques and for which a similar set of variables was available, including individual data on age, gender, ethnicity, batch, smoking habits, BMI, and season of blood collection, and a limited set of gene variants.. Results: Lowest DNA adduct levels (P = 0.006) were observed in the spring (median = 0.50 adducts per 108 nucleotides), followed ...
In the present condition of the technique of cultivation of tissues, the only possible way of studying leucocytic secretions was to grow colonies of leucocytes in a medium of known properties and to examine the modifications of these properties under the influence of the living cells. The method was far from perfect, because the secretions were mixed with serum and accumulated for 48 hours in a medium where they probably underwent partial destruction. But an approximate idea of certain of the qualities of the secretions, although not of their quantity, could be derived from the experiments. In the fluids extracted from the cultures, we attempted to detect the presence of the leucocytic secretions through their physiological effects on homologous and foreign cells. Two kinds of substances were sought, those which act on homologous cells, and those which destroy foreign erythrocytes.. The secretion by leucocytes of substances necessary to the nutrition of other cells was considered as probable ...
By a News Reporter-Staff News Editor at VerticalNews Science -- DURHAM, N.C. - Physicians and patients who are wary of addiction to pain medication and opioids may soon have a healthier and more natural alternative. A Duke University study revealed that a derivative of DHA (docosahexaenoic acid), a main ingredient of over-the-counter fish oil supplements, can sooth and prevent neuropathic pain caused by injuries to the sensory system. The results appear online in the Annals of Neurology. The research focused on a compound called neuroprotectin D1=protectin D1 (NPD1=PD1), a bioactive lipid produced by cells in response to external stimuli. NPD1=PD1 is present in human white blood cells, and was first identified based on its ability to resolve abdominal and brain inflammation. These compounds are derived from omega-3 fatty acids found in fish oil, but are 1,000 times more potent than their precursors in reducing inflammation, said Ru-Rong Ji, professor of anesthesiology and neurobiology at Duke ...
TY - JOUR. T1 - Leukocyte differentiation antigens in canine cutaneous and oral plasmacytomas. AU - Schrenzel, Mark D.. AU - Naydan, Diane K.. AU - Moore, Peter F. PY - 1998/3. Y1 - 1998/3. N2 - Seventeen cutaneous and oral tumours with light microscopic features of plasmacytomas from 16 dogs were studied. Clinically, most neoplasms were benign, although three recurred after excision and three were locally invasive. Tumours most often arose on the pinnae, digits, gingiva, and inguinal regions near areas of chronic inflammation and exhibited variable degrees of plasmacytic differentiation microscopically. Diagnosis of plasmacytoma was confirmed in paraffin-embedded tissues with a panel of leukocyte differentiation antigen markers that included cross-reactive antibodies for Mb-1 (CD79a), CD3, and vimentin and canine-specific antibodies for CD45RA and CD18. Immunoreactivity for Mb-1 and CD45RA, including staining of multinucleate cells and cells with karyomegaly, confirmed a B-cell origin of ...
The blood delivers leukocytes to the various tissues from the bone marrow, where they are produced. Normal blood leukocyte counts are 4.3-10.8 × 109/L, with neutrophils representing 45-74% of the cells, bands 0-4%, lymphocytes 16-45%, monocytes 4-10%, eosinophils 0-7%, and basophils 0-2%. Variation among individuals and among different ethnic groups can be substantial, with lower leukocyte numbers for certain African-American ethnic groups. The various leukocytes are derived from a common stem cell in the bone marrow. Three-fourths of the nucleated cells of bone marrow are committed to the production of leukocytes. Leukocyte maturation in the marrow is under the regulatory control of a number of different factors, known as colony-stimulating factors (CSFs) and interleukins (ILs). Because an alteration in the number and type of leukocytes is often associated with disease processes, total white blood cell (WBC) count (cells per μL) and differential counts are informative. This chapter focuses on ...
A randomized study comparing leukocyte-depleted versus packed red cell transfusions in prospective cadaver renal allograft recipients.
In the 9 years since the last review on leukocyte and endothelial interactions was published in this journal many of the critical structures involved in leukocyte adherence to and migration across endothelium have been elucidated. With the advent of cell and molecular biology approaches, investigations have progressed from the early descriptions by intravital microscopy and histology, to functional and immunologic characterization of adhesion molecules, and now to the development of genetically deficient animals and the first phase I trial of anti-adhesion therapy in humans. The molecular cloning and definition of the adhesive functions of the leukocyte integrins, endothelial members of the Ig gene superfamily, and the selectins has already provided sufficient information to construct an operative paradigm of the molecular basis of leukocyte emigration. The regulation of these adhesion molecules by chemoattractants, cytokines, or chemokines, and the interrelationships of adhesion pathways need ...
NK cells have therapeutic potential for a wide variety of human malignancies. However, because NK cells expand poorly in vitro, have limited life spans in vivo, and represent a small fraction of peripheral white blood cells, obtaining sufficient cell numbers is the major obstacle for NK-cell immunot …
Ending your shower in cold water improves circulation. It helps bring the blood back from the surface of the skin to all the internal organs. This process supports the prevention of cardiovascular disease by decreasing inflammation. Cold water contrast also stimulates metabolism, increasing oxygen absorption and carbon dioxide excretion. Carbon dioxide can actually be very toxic to the body when we dont get rid of it efficiently. (This is why deep breathing is so important.) It also increases nitrogen absorption and excretion, increases tissue tone, peripheral white blood cell and red blood cell counts, and decreases blood glucose. In other words, this routine will support many different functions of digestive and cardiovascular health as well as immunity ...
Ending your shower in cold water improves circulation. It helps bring the blood back from the surface of the skin to all the internal organs. This process supports the prevention of cardiovascular disease by decreasing inflammation. Cold water contrast also stimulates metabolism, increasing oxygen absorption and carbon dioxide excretion. Carbon dioxide can actually be very toxic to the body when we dont get rid of it efficiently. (This is why deep breathing is so important.) It also increases nitrogen absorption and excretion, increases tissue tone, peripheral white blood cell and red blood cell counts, and decreases blood glucose. In other words, this routine will support many different functions of digestive and cardiovascular health as well as immunity ...
PECAM-1 is a well-studied cellular adhesion and signaling receptor that plays an important role in supporting leukocyte diapedesis during the leukocyte adhesion cascade (4, 21). In contrast to this proinflammatory effect, PECAM-1 was shown in a number of situations to function as an ITIM-containing inhibitory receptor capable of dampening cellular activation events in lymphocytes (9, 22, 23), mast cells (24), and platelets (25-28). Numerous reports also demonstrated an anti-inflammatory role for PECAM-1 in well-established acute and chronic inflammatory disease models. For example, mice expressing PECAM-1 produce lower levels of inflammatory cytokines (7, 11-13), exhibit enhanced vascular barrier protection (10-12), and paradoxically, accumulate fewer leukocytes at sites of inflammation (10-13). However, the mechanisms by which PECAM-1 serves to confer protection in inflammation and how it regulates these aspects of the inflammatory response are still poorly understood.. One intriguing ...
The Mayo Clinic study is the first to identify an atrial fibrillation-associated genetic mutation of the ATP-sensitive potassium (KATP) channel. Researchers uncovered its role as a safeguard against atrial arrhythmia under stress conditions. The fail-safe mechanism present in most people to provide electrical stability to the heart under stress was defective in this patient. The sequencing of KATP channel genes, using genomic DNA extracted from the patients peripheral white blood cells, revealed a genetic mutation.. The discovery of the genetic mutations role in contributing to atrial fibrillation may ultimately improve physicians ability to identify patients who have a hereditary predisposition to atrial fibrillation, which is often complicated by increased risk for stroke and heart failure. Our findings support the emerging understanding of atrial fibrillation in younger patients as an inherited disease of ion channels, the building blocks of electrical pathways, says Timothy Olson, M.D., ...
- know what leukocytes are - know the functions of leukocytes - know the process of leukocpoiesis - know what the values for leukocytes are - understand the importance of a differential leukocyte count - understand: leukocytosis, leukocytopenia, leukemia basics This packet covers the topic of leukocytes
EZ Blood/Cell DNA Isolation Kit-EZ Blood/Cell DNA Isolation Kit is designed for isolating genomic DNA from whole blood or cells. The kit is based on s
In some countries, including India, the belief persists that watching a solar eclipse may be so stressful that it is bad for ones health. British researchers Omar Mian at Manchester University and Rubina Mian and Doug Thake at Coventry University were skeptical of tales about eclipses making people sick, and even causing deformities in unborn babies. To find out if there was any evidence either way, Rubina Mian took her graduate students to a field in Briey, France, to watch the 1999 summer eclipse. By analysing their blood samples with a luminometer, the researchers found that leukocyte activity increases by 8.7 percent during the eclipse. These white blood cells usually help our immune system, but if overstimulated they can damage DNA by releasing free radicals. Experiments after the eclipse showed that darkness, silence and temperature had no effect on leukocyte activity. But in other studies being prepared for publication, Rubina Mian has found that stress can have a big effect.. If ...
PolySia expression and function have been extensively studied in the nervous system, but the distribution and role of polySia in the immune compartment is still undefined. Herein we demonstrated the presence of polySia on human NK cells and its absence on other immune subsets, a finding that is in accord with two earlier reports (26, 27). NK cells up-regulated the expression of polySia and its scaffold NCAM upon activation by IL-2 treatment. This coregulation suggested that the heightened polySia signals were due to an up-regulation of both scaffold and glycan, rather than solely attributable to expanded polySia chain length. In support of this conclusion, a comparison of DP on resting and activated cells showed that upon activation total polySia was increased and the DP became more homogenous, tending toward small- to medium-length chains. To our knowledge, this is the first elucidation of polySia DP on primary human cells. The DP values we observed were slightly larger than analogous ...
The mechanisms of leukocyte recruitment have been studied extensively in vitro and have shed light on the basic molecular structure-function relationship of adhesion and signaling molecules involved in this essential immune response. This review will summarize how these in vitro observations extend to leukocyte behavior in inflamed blood vessels in the microcirculation. We highlight physiological results that might not have been predicted from in vitro systems. Special attention is placed on the physiology of rolling, adhesion, and intralumenal crawling in blood vessels. The importance of the glycocalyx, secondary tethers, shear, and the microenvironment are discussed. Docking structures forming rings of adhesion molecules together with a novel endothelial dome-like structure in vivo during transmigration are highlighted. Transcellular and paracellular emigration out of inflamed blood vessels is also discussed. The last section highlights leukocyte recruitment in some organs that do not always ...
The leukocyte adhesion cascade is an important paradigm of immunity and mediates leukocyte recruitment in acute or chronic inflammatory responses. Leukocyte recruitment requires several adhesive interactions between leukocytes and endothelial cells. The adhesion of leukocytes to the endothelial cell surface is mediated by interactions between leukocyte integrins, such as the beta1-integrin family member VLA-4 (a4b1) or the beta2-integrin family members LFA-1 (aLb2, CD11a/CD18), Mac-1 (aMb2, CD11b/CD18, complement receptor-3), and their endothelial counter-receptors of the immunoglobulin superfamily (ICAM-1, VCAM-1) (1). Our lab has made significant contributions to the leukocyte adhesion cascade, including the recent identification of a novel endogenous inhibitor of leukocyte recruitment, the endothelial-derived molecule Developmental Endothelial Locus-1 (Del-1, Edil3) (2-4).. Mobilization of hematopoietic stem cells (HSC) from the bone marrow to the periphery takes place upon infection. HSC ...
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White blood cells (also called leukocytes or leucocytes and abbreviated as WBCs) are the cells of the immune system that are involved in protecting the body against both infectious disease and foreign invaders. All white blood cells are produced and derived from multipotent cells in the bone marrow known as hematopoietic stem cells. Leukocytes are found throughout the body, including the blood and lymphatic system.[1] All white blood cells have nuclei, which distinguishes them from the other blood cells, the anucleated red blood cells (RBCs) and platelets. Types of white blood cells can be classified in standard ways. Two pairs of broadest categories classify them either by structure (granulocytes or agranulocytes) or by cell lineage (myeloid cells or lymphoid cells). These broadest categories can be further divided into the five main types: neutrophils, eosinophils (acidophiles), basophils, lymphocytes, and monocytes.[2] These types are distinguished by their physical and functional ...
Introduction: Psoriasis vulgaris (PV) is a chronic skin disease, mediated by environmental risk factors and aberrant immune response to unknown antigens. CD8+ cytotoxic lymphocytes make a large proportion of the leukocyte infiltrate. Their secretion of IL-17/IFN-γ/TNF-α helps in growing inflammatory state in a skin, keratinocytes proliferation and dedifferentiation and consequently, forming scaly plaques. Objectives: To examine and compare frequency of CD3+CD8+ T lymphocytes in PV patients and healthy controls and determine their association with demographic and clinical features of psoriasis. Material and methods: Peripheral blood was collected from 21 PV patients and 22 healthy controls. Peripheral leukocytes were isolated during centrifugation in density gradient Lymphoprep™ medium. Dead cells were excluded with „LIVE/DEAD™ Fixable Dead Cell Stain Kit. Immunophenotyping CD3+CD8+ T lymphocytes was performed on a flow cytometry (BD FACS Canto II) after labeling samples with anti-human ...
Clone 390 reacts with the cell surface protein CD31. The murine CD31 antigen is also known as PECAM-1. The encoded protein is a single-pass type I membrane protein containing six Ig-like C2-type (immunoglobulin-like) domains and functions as cell adhesion molecule. CD31 is present on mature endothelial cells as well as on most leukocyte subtypes and platelets where the expression level can vary. Besides its function in exhibiting adhesive properties, the protein is required for leukocyte transendothelial migration (TEM) under most inflammatory conditions. | Belgique
The liver plays a major role for the metabolism, but it is also of general importance for the immune system, e.g. for the deletion of activated T cells or the induction of peripheral tolerance. Under physiological conditions T cells and other leukocytes can be found in the liver, in the sinusoids as well as in the parenchyma. This hepatic accumulation of T cells might be due to immunosurveillance, but it would also be a prerequisite for modulation of T cells by hepatic cells. The present study investigated two different aspects of the interaction of liver sinusoidal endothelial cells (LSEC), the barrier between the sinusoidal lumen and the hepatic parenchyma, and CD4+ T cells. In the first part of the study it could be demonstrated that LSEC support the spontaneous transmigration of CD4+ T cells as well as their chemotaxis to CXCL12 and CXCL9 more efficiently than other endothelial cells. Whereas a direct endothelial activation by chemokines could be excluded the efficient chemokine presentation ...
Aside from nutritional significance, milk affords infant mammals immunologic benefits. However, it is not without immunologically based hazards. These stem from its antigenicity and the fact that in certain species that receive their maternal immunologic endowment postpartum, hemolytic disease of the newborn may be mediated by colostral antibodies. Awareness that viable leukocytes are ingredients of colostrum and milk has stimulated interest in the significance of these cells. Skin grafting tests on foster-nursed rats and mice have given circumstantial evidence that, in these species, leukocytes may be transmitted naturally from the mothers blood stream to the sucklings blood stream through the milk, and that these cells may be beneficial (adoptive immunization) or, in some genetic contexts, harmful (initiating graft-versus-host disease). In man, too, studies on necrotizing enteritis and other diseases provide increasing support for the thesis that leukocytes in milk fulfill a protective ...
TY - GEN. T1 - TGV-based flow estimation for 4D leukocyte transmigration. AU - Frerking, L.. AU - Burger, M.. AU - Vestweber, D.. AU - Brune, Christoph. PY - 2014/8/26. Y1 - 2014/8/26. N2 - The aim of this paper is to track transmigrating leukocytes via TGV flow estimation. Recent results have shown the advantages of the nonlinear and higher order terms of TGV regularizers, especially in static models for denoising and medical reconstruction. We present TGV-based models for flow estimation with the goal to get an exact recovery of simple intracellular and extracellular flows, as well as its implication on realistic tracking situations for transmigration through barriers. To study and quantify different pathways of transmigrating leukocytes, we use large scale 4D fluorescence live microscopy data in vivo.. AB - The aim of this paper is to track transmigrating leukocytes via TGV flow estimation. Recent results have shown the advantages of the nonlinear and higher order terms of TGV regularizers, ...
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© 2016 International Union of Biochemistry and Molecular Biology Type 1 diabetes mellitus (T1DM) results from immune cell-mediated reductions in function and mass of the insulin-producing β-cells within the pancreatic islets. While the initial trigger(s) that initiates the autoimmune process is unknown, there is a leukocytic infiltration that precedes islet β-cell death and dysfunction. Herein, we demonstrate that genes encoding the chemokines CXCL9, 10, and 11 are primary response genes in pancreatic β-cells and are also elevated as part of the inflammatory response in mouse, rat, and human islets. We further established that STAT1 participates in the transcriptional control of these genes in response to the pro-inflammatory cytokines IL-1β and IFN-γ. STAT1 is phosphorylated within five minutes after β-cell exposure to IFN-γ, with subsequent occupancy at proximal and distal response elements within the Cxcl9 and Cxcl11 gene promoters. This increase in STAT1 binding is coupled to the rapid
Mouse monoclonal antibody raised against native SELL. Native purified SELL from PMA-activated human peripheral blood leukocytes. (MAB9801) - Products - Abnova
Cellular hypersensitivity to an extract of human pancreas, using the leucocyte migration test (LMT), was found in twenty-nine of 101 diabetic and eight of fifty normal control subjects. However, the difference in response between diabetics and controls was confined to young insulin-dependent patients, there being no distinction between normal subjects and older diabetics treated by diet or oral hypoglycemic agents. The use of rat liver mitochondria and bovine insulin as antigens in the LMT did not induce inhibition of leucocyte migration in diabetics or controls.. ...
GUARDA, Ismael Francisco Mota Siqueira; CORREIA, Cristiano Jesus; BREITHAUPT-FALOPPA, Ana Cristina; et al. Effects of ethyl pyruvate on leukocyte-endothelial interactions in the mesenteric microcirculation during early sepsis treatment. Clinics, São Paulo, v. 70, n. 7, p. 508-514, 2015. Disponível em: < http://www.scielo.br/pdf/clin/v70n7/1807-5932-clin-70-07-508.pdf > DOI: 10.6061/clinics/2015(07)08 ...
Troubles with leukocyte adhesion - what to do? - posted in Tissue and Cell Culture: Hello! I am working with living leukocytes, which I use for surface-enhanced Raman spectroscopy (SERS) and it is crucial that the cells are as still as possible in order for me to create a spectral image. The leukocytes are directly extracted from human donor blood and then fronzen in liquid nitrogen (I am not doing this procedure myself).I have been trying to attach them to the well bottom (glass) b...
TY - JOUR. T1 - Binding of human leukocytes to fibronectin is augmentd by an anti-CD44 mAb(TL-1)anlocked by another anti CD44 mAb(Hermes-3)but not by anti-VLA4/VLA-5 mAbs(共). AU - Yoshino, Tadashi. PY - 1997. Y1 - 1997. M3 - Article. VL - 196. SP - 504. EP - 512. JO - Immunobiol. JF - Immunobiol. ER - ...
Octanoate and palmitate beta-oxidation in human leukocytes: implications for the rapid diagnosis of fatty acid beta-oxidation disorders.:
White blood cells, or leucocytes (also spelled leukocytes, from the Greek word leuko-, meaning white), are a component of blood. As part of the bodys immune system, their function is to protect the body from disease and infection if pathogens get in the bloodstream. White blood cells come in several varieties, each with a different role to play in neutralizing harmful foreign substances. White blood cells, like all blood cells, are produced in the bone marrow. They are also known as leukocytes. There are many types of leukocytes, and these types are produced from immature cells in the lymphatic system in response to the bodys needs. For example, infection with bacteria will cause more macrophages to be produced and fewer NK cells, in response to alarm signals called chemokines. Macrophages then exit the bloodstream, migrate towards the site of infection, and attempt to neutralize the invading pathogens. Others begin producing antibodies or recruit other systems to help. The immune system of ...
The immune system works through leukocytes interacting with each other, with other cells, with tissue matrices, with infectious agents, and with other antigens. These interactions are mediated by cell-surface glycoproteins and glycolipids. Antibodies against these leukocyte molecules have provided powerful tools for analysis of their structure, function, and distribution. Antibodies have been used widely in hematology, immunology, and pathology, and in research, diagnosis, and therapy. The associated CD nomenclature is commonly used when referring to leukocyte surface molecules and antibodies against them. It provides an essential classification for diagnostic and therapeutic purposes. The most recent (8th) Workshop and Conference on Human Leukocyte Differentiation Antigens (HLDA), held in Adelaide, Australia, in December 2004, allocated 95 new CD designations and made radical changes to its aims and future operational strategy in order to maintain its relevance to modern human biology and clinical
Biological measures of aging are important for understanding the health of an aging population, with epigenetics particularly promising. Previous studies found that tumor tissue is epigenetically older than its donors are chronologically. We examined whether blood Δage (the discrepancy between epigenetic and chronological ages) can predict cancer incidence or mortality, thus assessing its potential as a cancer biomarker. In a prospective cohort, Δage and its rate of change over time were calculated in 834 blood leukocyte samples collected from 442 participants free of cancer at blood draw. About 3-5 years before cancer onset or death, Δage was associated with cancer risks in a dose-responsive manner (P = 0.02) and a one-year increase in Δage was associated with cancer incidence (HR: 1.06, 95% CI: 1.02-1.10) and mortality (HR: 1.17, 95% CI: 1.07-1.28). Participants with smaller Δage and decelerated epigenetic aging over time had the lowest risks of cancer incidence (P = 0.003) and mortality ...
An improved multi-purpose blood diluent for use with a gentle lysing agent and improved detergent reagent system are disclosed which are especially suitable for use in routine electronic enumeration and volumetric differentation of blood cells. The preferred imidazole stabilizer used in the diluent reagent is found to be an excellent cell stabilizing agent and buffer for maintaining cell morphology during operation. A synergistic combination of a superior antimicrobial agent, the preferred Triadine-10, used in the diluent and the detergent reagents, not only prevents bacterial or fungal growth, but also helps to stabilize cells and to obtain distinct volumetric differentiation of certain leukocyte subpopulations. The preferred Brij 35 in a balanced salt solution has proved to be an efficient and cost effective detergent to ensure accurate results and trouble-free operation of the analyzers.
Yesterday we talked about red blood cells, and today we will talk about white blood cells. White blood cells, or leukocytes are the cells of the immune system. They are what helps your body to fight infection and foreign matter. The most common type of white cell is the Neutrophil. Neutrophils live in the body…
The invention relates generally to phosphoinositide 3-kinases (Pl3Ks), and more particularly to methods of inhibiting leukocyte accumulation comprising selectively inhibiting phosphoinositide 3-kinase delta (Pl3Kδ) activity in endothelial cells.
Helpful, trusted answers from doctors: Dr. Dohan on white blood cells in urine no infection: White blood cells in urine usually are there because of an infection in the urinary tract. However, they can also be there in large numbers in a female when there is a vaginal infection or when she is having her period (always see red cells also in this situation). My best advice is to see your doctor and have a urine culture done to see what is going on.
Mouse monoclonal antibody raised against native CD34. Native purified CD34 from permanent human cell line derived from peripheral leucocytes of a patient suffering from chronic myeloid leukaemia. (MAB4412) - Products - Abnova
Dr Marlies Reinders is a Nephrologist and has long experience working on alloimmunity and renal transplantation. She performed research focused on leukocyte-endothelial cell biology in transplantation at the Transplant Immunology Laboratory, ...
Stimulation of blood leukocytes[edit]. 13-HODE (and 9-HODE) are moderately strong stimulators of the directed migration (i.e. ... of human polymorphonuclear leukocytes". The Journal of Biological Chemistry. 260 (7): 4508-15. PMID 3920219.. .mw-parser-output ... to a far greater extent than any other type of leukocyte.[64] The mechanism responsible for 13-HODE's impact on airway ... "Oxidative metabolism of linoleic acid by human leukocytes". Biochemical and Biophysical Research Communications. 161 (2): 883- ...
Example: Human leukocyte antigen (HLA) alleles[edit]. HLA constitutes a group of cell surface antigens also known as the MHC of ...
Human leucocyte antigen polymorphisms[edit]. Main article: Human leukocyte antigen. Human leucocyte antigen (HLA) polymorphisms ... leucocyte - white blood cell, part of the immune system, which together with red blood cells, comprise the cellular component ... erythrocyte - red blood cell, which with the leucocytes make up the cellular content of the blood (contrast leucocyte) ... also called the human leucocyte antigen (HLA) system ... 3.3 Human leucocyte antigen polymorphisms. *3.4 Hereditary ...
Leukocytes ...Pancreatic islet β cells ... Primary Tonsillar B Cells ... Circulating leukocytes of healthy subjects ( ... Phytohaemagglutinin upregulates hTAAR1 mRNA in circulating leukocytes; in these cells, TAAR1 activation mediates leukocyte ... Babusyte A, Kotthoff M, Fiedler J, Krautwurst D (March 2013). "Biogenic amines activate blood leukocytes via trace amine- ... Response measured: cAMP accumulation ... Activation of leukocytes Species: Human Tissue: PMN, T and B cells Response measured: ...
"Cage:Leukocytes". Guilty Crown. Episode 6. November 17, 2011. Fuji TV. "Degeneracy:Retraction". Guilty Crown. Episode 10. ...
The mechanism of leukocyte locomotion. In: Gallin JL, Quie PG, eds. Leukocyte Chemotaxis. New York: Raven Press, 1978:143-57. ... The motor of leukocytes. Fed Proc. 1984; 43:2760-2763. 56. Stossel TP. Contribution of actin to the cytoplasmic matrix. J. Cell ... The motor of leukocytes and platelets. In: The Molecular Biology of the Arterial Wall. G. Schettler, ed., Springer Verlag. 1987 ... Leukocytes II. Phagocytosis and its disorders. In: Beck WS, ed. Hematology. Cambridge, MA: MIT Press, 1977:361-373. 25. Stossel ...
In common parlance, the term polymorphonuclear leukocyte often refers specifically to "neutrophil granulocytes",[2] the most ... Gleich, Gerald J.; Adolphson, Cheryl R. (1986). "The Eosinophilic Leukocyte: Structure and Function". Advances in Immunology ... Injured basophils and other leukocytes will release another substance called prostaglandins that contributes to an increased ... They are also called polymorphonuclear leukocytes (PMN, PML, or PMNL) because of the varying shapes of the nucleus, which is ...
Prostaglandin F2α (PGF2α in prostanoid nomenclature), pharmaceutically termed carboprost, is a naturally occurring prostaglandin used in medicine to induce labor and as an abortifacient.[1] In domestic mammals, it is produced by the uterus when stimulated by oxytocin, in the event that there has been no implantation during the luteal phase. It acts on the corpus luteum to cause luteolysis, forming a corpus albicans and stopping the production of progesterone. Action of PGF2α is dependent on the number of receptors on the corpus luteum membrane. The PGF2α isoform 8-iso-PGF2α was found in significantly increased amounts in patients with endometriosis, thus being a potential causative link in endometriosis-associated oxidative stress.[2] ...
... , as a lipophilic ester, easily penetrates the cornea and is then activated to the carboxylic acid, tafluprost acid. Onset of action is 2 to 4 hours after application, the maximal effect is reached after 12 hours, and ocular pressure remains lowered for at least 24 hours.[2][3] Tafluprost acid is inactivated by beta oxidation to 1,2-dinortafluprost acid, 1,2,3,4-tetranortafluprost acid, and its lactone, which are subsequently glucuronidated or hydroxylated. The cytochrome P450 liver enzymes play no role in the metabolism.[3] An analogous pathway (at least up to the tetranor-metabolites) has been found for latanoprost and travoprost. ...
It is believed that the vasoconstriction caused by thromboxanes plays a role in Prinzmetal's angina. Omega-3 fatty acids are metabolized to produce higher levels of TxA,3 which is relatively less potent than TxA2 and PGI3; therefore, there is a balance shift toward inhibition of vasoconstriction and platelet aggregation. It is believed that this shift in balance lowers the incidence of myocardial infarction (heart attack) and stroke. Vasoconstriction and, perhaps, various proinflammatory effects exerted by TxA on tissue microvasculature, is probable reason why the TxA is pathogenic in various diseases, such as ischemia-reperfusion injury.,[2] hepatic inflammatory processes,[3] acute hepatotoxicity [4] etc. TxB2, a stable degradation product of TxA2, plays a role in acute hepatoxicity induced by acetaminophen.[5][6] ...
The US National Institute of Health's MedlinePlus lists medical conditions for which EPA (alone or in concert with other ω-3 sources) is known or thought to be an effective treatment.[18] Most of these involve its ability to lower inflammation. Intake of large doses (2.0 to 4.0 g/day) of long-chain omega-3 fatty acids as prescription drugs or dietary supplements are generally required to achieve significant (, 15%) lowering of triglycerides, and at those doses the effects can be significant (from 20% to 35% and even up to 45% in individuals with levels greater that 500 mg/dL). It appears that both EPA and DHA lower triglycerides; however, DHA appears to raise low-density lipoprotein (the variant which drives atherosclerosis, sometimes inaccurately called "bad cholesterol") and LDL-C values (always only a calculated estimate; not measured by labs from person's blood sample for technical and cost reasons), while EPA does not. EPA and DHA ethyl esters (all forms) may be absorbed less well, thus ...
Journal of Leukocyte Biology. 43 (2): 117-24. doi:10.1002/jlb.43.2.117. PMID 3422086.. ... a form of blood circulating leukocyte, increases its expression of CYP2S1 when forced to differentiate into a macrophage ...
The drug is clear with a pH of 10.[7] Its production is inhibited indirectly by NSAIDs, which inhibit the cyclooxygenase enzymes COX1 and COX2. These convert arachidonic acid to prostaglandin H2 (PGH2), the immediate precursor of prostacyclin. Since thromboxane (an eicosanoid stimulator of platelet aggregation) is also downstream of COX enzymes, one might think that the effect of NSAIDs would act to balance. However, prostacyclin concentrations recover much faster than thromboxane levels, so aspirin administration initially has little to no effect but eventually prevents platelet aggregation (the effect of prostaglandins predominates as they are regenerated). This is explained by understanding the cells that produce each molecule, TXA2 and PGI2. Since PGI2 is primarily produced in a nucleated endothelial cell, the COX inhibition by NSAID can be overcome with time by increased COX gene activation and subsequent production of more COX enzymes to catalyze the formation of PGI2. In contrast, TXA2 is ...
... supplementation in daily doses of 1,000-1,500 mg for 50 days has been well tolerated during several clinical studies, with no significant side effects reported. All common markers of health, including kidney and liver function,[35] serum lipids,[39] immunity,[40] and platelet aggregation[34] appear to be unaffected with this level and duration of use. Furthermore, higher concentrations of ARA in muscle tissue may be correlated with improved insulin sensitivity.[41] Arachidonic acid supplementation of the diets of healthy adults appears to offer no toxicity or significant safety risk. While studies looking at arachidonic acid supplementation in sedentary subjects have failed to find changes in resting inflammatory markers in doses up to 1,500 mg daily, strength-trained subjects may respond differently. One study reported a significant reduction in resting inflammation (via marker IL-6) in young men supplementing 1,000 mg/day of arachidonic acid for 50 days in combination with ...
Alprostadil is sold in the United States as urethral suppositories and in injectable form. The suppositories are sold under the brand name Muse.[6] The injectable forms are Edex[7] and Caverject.[8] Muse delivers alprostadil as a penile suppository, inserted into the urethra, at least ten minutes before the erection is needed. Caverject and Edex are similarly fast-acting, but instead are injected by syringe directly into the corpus cavernosum of the penis. Alprostadil is also available as a generic. The major cost is that it must be mixed by a compounding pharmacy and supplies may be difficult to obtain. The different formulations, including Bimix and Trimix, may include papaverine and/or phentolamine. A typical mix might be 30 mg of papaverine, 2 mg of phentolamine, and 20 μg alprostadil. As a generic, it is much less expensive than the packaged injectables. It is premixed and must be kept refrigerated and the user must load a syringe with the quantity needed. Most recently, the compound has ...
chemotactic factor for and activator of leukocytes; inflammation. studies to date shown no clear benefits of LTB4 receptor ...
ISBN 978-0-8036-6825-6. Mary Louise Turgeon (14 April 2014). "Leukocytes and platelets". Linne & Ringsrud's Clinical Laboratory ... Qualitative disorders of leukocytes". Wintrobe's Clinical Hematology. 1 (12th ed.). Philadelphia, PA: Lippincott Williams & ...
Tumor-Associated Leukocytes. 1994. Chemokines. 1999. Pharmacology of cytokines. 2000. Gianfranco Bazzoni; Elisabetta Dejana; ...
Monocytes, Leukocytes Stimulate synthesis of IL-2 10 Interleukin-2 Lymphocytes Stimulate growth and maturation of T-cells ...
PMN: "polymorphonuclear leukocytes". *OCA: "oculocutaneous albinism". *PCM: "paracoccidioidomycosis". *Pronounced as a word or ...
"Episode 6: "Cage:Leukocytes"". guilty-crown.jp (in Japanese). Retrieved January 22, 2017. ギルティクラウン (in Japanese). Web Newtype. ...
Leukocytes and Host Defense. Progress in Leukocyte Biology. 5. New York: Alan R. Liss. p. 200. Fairchild SS, Shannon K, Kwan E ... reflecting leukocyte redistribution to lymph nodes, bone marrow, and skin). Rapid administration of corticosterone (the ... to adrenalectomized animals induced changes in leukocyte distribution. Natural killer cells are affected by cortisol. Cortisol ...
This consists of fibrin strands and leukocytes. Fibrin describes an amorphous, eosinophilic (pink) network. Leukocytes (white ...
Record sleeve Leucocyte. Esbjörn Svensson Trio. The ACT Company. Red Dot Design Award 2010. Book design Freud med Skalpell av ... Leucocyte. Esbjörn Svensson Trio. The ACT Company. German Design Award 2012 Nominee. Corporate Design. Sollentuna Shopping ...
Leukapheresis - leukocytes (white blood cells). Leukopheresis is the removal of PMNs, basophils, eosinophils for transfusion ...
Leukocytes generate HCN during phagocytosis. The vasodilatation, caused by sodium nitroprusside, has been shown to be mediated ... and leukocyte adhesion to the endothelium. Humans with atherosclerosis, diabetes, or hypertension often show impaired NO ...
Moore GE, Gerner RE, Franklin HA (1967). "Culture of normal human leukocytes". JAMA. 199 (8): 519-524. doi:10.1001/jama.199.8. ...
Leukocytes and platelets are normal. Bone marrow shows erythroid hyperplasia with a maturation arrest. In excess of 40% of the ...
Indium leukocyte imaging is better for acute infections (where neutrophils are still rapidly and actively localizing to the ... Both the gallium scan and indium leukocyte imaging may be used to image fever of unknown origin (elevated temperature without ... In infections, the gallium scan has an advantage over indium leukocyte imaging in imaging osteomyelitis (bone infection) of the ... It has been suggested that gallium imaging may become an obsolete technique, with indium leukocyte imaging and technetium ...
Bennett RM, Gabor GT, Merritt MM (December 1985). "DNA binding to human leukocytes. Evidence for a receptor-mediated ... "The production and characterization of murine monoclonal antibodies to a DNA receptor on human leukocytes". Journal of ...
Leukocyte adhesion deficiency[edit]. Main article: Leukocyte adhesion deficiency. Leukocyte adhesion deficiency (LAD) is a ... Leukocyte extravasation, less commonly called diapedesis, is the movement of leukocytes out of the circulatory system and ... Transmigration of the leukocyte occurs as PECAM proteins, found on the leukocyte and endothelial cell surfaces, interact and ... The cytoskeletons of the leukocytes are reorganised in such a way that the leukocytes are spread out over the endothelial cells ...
List of human leukocyte antigen alleles associated with cutaneous conditions. References[edit]. *^ Galbraith W, Wagner MC, Chao ... The human leukocyte antigen (HLA) system or complex is a gene complex encoding the major histocompatibility complex (MHC) ... Human leukocyte antigens at the US National Library of Medicine Medical Subject Headings (MeSH) ... Retrieved from "https://en.wikipedia.org/w/index.php?title=Human_leukocyte_antigen&oldid=805696217" ...
Spitznagel J.K. (1984) Nonoxidative Antimicrobial Reactions of Leukocytes. In: Snyderman R. (eds) Regulation of Leukocyte ... I. L. pneumophila resists killing by polymorphonuclear leukocytes, antibody and complement, J. Exp. Med. 153:386.PubMedCrossRef ... Elastinolytic activity in granules of human polymorphonuclear leukocytes, J. Exp. Med. 128:1137.PubMedCrossRefGoogle Scholar ... Characterization of the bactericidal substance in leucocyte mitochondrial extracts, J. Exp. Med. 105:529.PubMedCrossRefGoogle ...
leukocyte The Oxford Pocket Dictionary of Current English © The Oxford Pocket Dictionary of Current English 2009, originally ... "leukocyte." The Oxford Pocket Dictionary of Current English. . Encyclopedia.com. 14 Aug. 2018 ,http://www.encyclopedia.com,. ... www.encyclopedia.com/humanities/dictionaries-thesauruses-pictures-and-press-releases/leukocyte ... www.encyclopedia.com/humanities/dictionaries-thesauruses-pictures-and-press-releases/leukocyte ...
Create healthcare diagrams like this example called Leukocytes - Granulocytes in minutes with SmartDraw. SmartDraw includes ... Leukocytes - Granulocytes. Create healthcare diagrams like this example called Leukocytes - Granulocytes in minutes with ... Leukocytes - Granulocytes. Three granular or polymorphonuclear leukocytes: Basophil, Neutrophil, and Eosinophil. Red blood cell ...
Create healthcare diagrams like this example called Leukocytes - Agranulocytes in minutes with SmartDraw. SmartDraw includes ... Leukocytes - Agranulocytes. Create healthcare diagrams like this example called Leukocytes - Agranulocytes in minutes with ... Leukocytes - Agranulocytes. Three agranular leukocytes: B-Lymphocyte, T-Lymphocyte, and Macrophage. Red blood cell (upper left ...
Preservation of leukocytes by suspending in storage medium containing modified fluid gelatin, plasma, and a non-toxic buffer. ... The leukocytes can be dispersed in the composition in amounts varying from 107 to 109 cells per ml, preferably from 1 108 to 5 ... The leukocytes (neutrophils) used in these examples were prepared from whole human blood by drawing a 30 ml specimen of blood ... Stabilization of leukocytes. US6387695. Dec 22, 1998. May 14, 2002. Merck & Co., Inc.. DNA pharmaceutical formulations ...
... leukocyte) that is characterized histologically by its ability to be stained by neutral dyes and functionally by its role in ... Alternative Titles: heterophil, neutrophyllic leukocyte. Neutrophil, type of white blood cell (leukocyte) that is characterized ... particle-engulfing) white blood cells called neutrophilic leukocytes, which, distended by the compound of antibody and of ... Under these conditions, the neutrophilic leukocytes (white blood cells called neutrophilic because of their neutral staining ...
The recruitment of leukocytes from the blood is a prerequisite for their participation in inflammatory responses within the ... Human Endothelial Cell Leukocyte Adhesion Monocyte Migration Leukocyte Adhesion Molecule Leukocyte Adhesion Deficiency These ... Leukocyte adhesion molecule-1 (LAM-1, L-selectin) interacts with an inducible endothelial cell ligand to support leukocyte ... Two-step model of leukocyte-endothelial cell interaction in inflammation: Distinct roles for LECAM-1 and the leukocyte Rz ...
Leucocyte jazz review by Ian Patterson, published on October 19, 2008. Find thousands reviews at All About Jazz! ... Leucocyte Ad Interim; Leucocyte Ad Mortem; Leucocyte Ad Infinitum.. Personnel: Esbjorn Svensson: piano; Dan Berglkund: bass; ... Leucocyte - Ad Interim; Leucocyte - Ad Mortem; Leucocyte - Ad Infinitum. ... E.S.T. Extended Analysis Esbjorn Svensson Ian Patterson ACT Music United States e.s.t.: Leucocyte ...
Learn about the diagnosis and treatment of an infection, and what happens if a pregnant person has leukocytes in their urine. ... Leukocytes are white blood cells that help protect people from infection. They are not usually present in the urine, so when ... Leukocytes in urine. Leukocytes can sometimes show in urine tests.. An unusually high number of leukocytes in the urine ... Why are there leukocytes in my urine? What are the causes of leukocytes in the urine and what are the signs and symptoms? ...
The leukocyte group contains several different types of cell, each with their own functions and each with a particular ... or leukocytes, make up one of the three types of blood cell. The others are red blood cells and platelets. ... granular leukocytes have a distinctive method of movement which the agranular leukocytes lack. They extend parts of the cell ... The most common leukocyte is the neutrophil, so called because its small granules do not stain very darkly. It has three to ...
Urine Leukocyte Esterase. Normal urine does not contain any white blood cells. A quick way to detect white blood cells in urine ... If leukocyte esterase is found, microscopic analysis is usually indicated to confirm the findings as false positive tests are ... So the laboratory may report leukocytes in urine without the patient really having an infection. If the white blood cells ... is to look for leukocyte esterase, an enzyme found in the white blood cells. When a urine sample is sent to the laboratory for ...
I had my urine tested and the results came out 1+ Protein 2+ Leukocytes and 4+ Blood in my urine, should I be worried, as to ... Protein, Leukocytes and Blood in urine. I had my urine tested and the results came out 1+ Protein 2+ Leukocytes and 4+ Blood in ... I had my urine tested and the results came out 1+ Protein 2+ Leukocytes and 4+ Blood in my urine, should I be worried, as to ...
There is an increasing need to understand the leukocytes and soluble mediators that drive acute inflammation and bring about ... Characterisation of leukocytes in a human skin blister model of acute inflammation and resolution. *Jenner W ... There is an increasing need to understand the leukocytes and soluble mediators that drive acute inflammation and bring about ... Characterisation of leukocytes in a human skin blister model of acute inflammation and resolution. PLoS ONE, 9(3). https://doi. ...
Leukocyte adhesion deficiency type 1 is a disorder that causes the immune system to malfunction, resulting in a form of ... leukocytes). These integrins help leukocytes gather at sites of infection or injury. , where they contribute to the immune ... A hallmark of leukocyte adhesion deficiency type 1 is the lack of pus formation at the sites of infection. In people with this ... Leukocytes that lack these integrins cannot attach to the blood vessel wall or cross the vessel wall to contribute to the ...
Leukocyte esterase is a urine test to look for white blood cells and other signs of infection. ... Leukocyte esterase is a screening test used to detect a substance that suggests there are white blood cells in the urine. This ... Leukocyte esterase is a urine test to look for white blood cells and other signs of infection. ...
Upon detection of inflammation, leukocytes initiate a highly orchestrated sequence of events, whereby they attach and roll ...
The ability of the dead HeLa cells to stimulate leukocyte recruitment required the cells to have swollen nuclei and for cPLA2 ... These results indicate that necrotic cells can recruit leukocytes in a manner similar to cells in damaged tissues and suggest ... Nuclear swelling triggers the production of chemoattractants to recruit leukocytes to injured or dead tissue. ... Nuclear swelling triggers the production of chemoattractants to recruit leukocytes to injured or dead tissue. ...
The Innate Immunity Signal Transduction in Human Leukocytes is a research study to determine the response of immune cells from ...
... J. M. Huebner,1 R. R. Eversole,1 W. F. Jackson,1 C. D ... "Leukotriene C4 biosynthesis in isolated August rat peritoneal leukocytes," Mediators of Inflammation, vol. 5, no. 6, pp. 443- ...
... definition at Dictionary.com, a free online dictionary with pronunciation, synonyms and translation. ...
leucocyte synonyms, leucocyte pronunciation, leucocyte translation, English dictionary definition of leucocyte. also leu·co· ... leucocyte. (ˈluːkəˌsaɪt) or leukocyte. n. (Biochemistry) any of the various large unpigmented cells in the blood of vertebrates ... leucocyte - blood cells that engulf and digest bacteria and fungi; an important part of the bodys defense system. leukocyte, ... granulocyte - a leukocyte that has granules in its cytoplasm. monocyte - a type of granular leukocyte that functions in the ...
Eosinophilic leukocyte definition at Dictionary.com, a free online dictionary with pronunciation, synonyms and translation. ...
... Author. Sebastien Viatte, MD, PhD. Sebastien Viatte, MD, PhD ... HLA genes express their gene products on the surface of white blood cells (hence the name human leukocyte antigen, although ... The human leukocyte antigen (HLA) complex is synonymous with the human MHC. ... Imputing amino acid polymorphisms in human leukocyte antigens. PLoS One 2013; 8:e64683. ...
Leukocyte adhesion deficiency (LAD) is a rare primary immunodeficiency. The clinical picture is characterized by marked ... infections that are difficult to detect until they have progressed to an extensive level secondary to lack of leukocyte ... Leukocyte adhesion deficiency I can affect people of all racial groups. Leukocyte adhesion deficiency II has been reported only ... More than one leukocyte adhesion deficiency variant has been labeled leukocyte adhesion deficiency type III (LAD III), creating ...
... "leukocyte recruitment cascade" which is orchestrated by precise adhesion molecule expression on the cell surface of leukocytes ... Leukocyte Adhesion Molecules in Diabetic Retinopathy. Kousuke Noda,1,2 Shintaro Nakao,3 Susumu Ishida,1,2 and Tatsuro Ishibashi ... During inflammation leukocytes undergo sequential adhesive interactions with endothelial cells to migrate into the inflamed ... This paper summarizes the recent clinical and preclinical works on the roles of leukocyte adhesion molecules in DR. ...
The National Cancer Institute states that leukocytes are an immune cell.... ... Leukocytes are white blood cells such as granulocytes, monocytes and lymphocytes. ... If an excess of these leukocytes are found in urine, it can be a sign that there may be an infection present. In children, this ... A doctor needs to evaluate your child if you think she may have leukocytes in her urine. If your child has been taking extra ...
... , Cerebrospinal Fluid Cell Leukocyte, CSF White Blood Cell, CSF Cell Count, Pleocytosis, CSF WBC. ... CSF Leukocyte. CSF Leukocyte Aka: CSF Leukocyte, Cerebrospinal Fluid Cell Leukocyte, CSF White Blood Cell, CSF Cell Count, ... WBC # CSF, Leukocytes:NCnc:Pt:CSF:Qn, Leukocytes [#/volume] in Cerebral spinal fluid, Leukocytes:Number Concentration (count/ ... These images are a random sampling from a Bing search on the term "CSF Leukocyte." Click on the image (or right click) to open ...
  • ICAM-1-mediated signals activate an endothelial cell calcium flux and PKC, which are required for ICAM-1 dependent leukocyte migration. (genome.jp)
  • Stromal progenitor cells promote leukocyte migration through production of stromal-derived growth factor 1alpha: a potential mechanism for stromal progenitor cell-mediated enhancement of cellular recruitment to wounds. (wordnik.com)
  • Leukocyte migration from the circulation into target tissues is a multi-step process. (picsearch.com)
  • Leukocyte migration is a crucial process in both homeostatic and inflammatory conditions. (frontiersin.org)
  • In this review, we discuss the current knowledge of catecholamine and glucocorticoid regulation of leukocyte migration under homeostatic and stimulated conditions. (frontiersin.org)
  • Leukocyte Migration and Differentiation in COPD Patients Compared to Healthy Smokers and Healthy Non-smoking Subjects. (clinicaltrials.gov)
  • Three granular or polymorphonuclear leukocytes: Basophil, Neutrophil, and Eosinophil. (smartdraw.com)
  • A prominent histologic feature of Helicobacter pylori infection is a dense infiltration of polymorphonuclear leukocytes (PMNL) in gastric mucosa. (nih.gov)
  • Distinguished from mononuclear leukocytes, polymorphonuclear leukocytes have the varying shapes of the nucleus, which is usually lobed into three segments. (genengnews.com)
  • Neutrophils are the most abundant polymorphonuclear leukocytes, and the other types (eosinophils, basophils, and mast cells) have much lower populations. (genengnews.com)
  • Human peripheral blood polymorphonuclear leukocytes, when exposed to appropriate stimuli, generate significant amounts of superoxide anion (O-.2), a highly reactive molecule which is possibly involved in bacterial killing. (jci.org)
  • Since the subcellular localization and mechanism of activation of O-.2 generating systems are unknown, we have investigated superoxide dismutase-inhibitable cytochrome c reduction (attributable to O-.2) by, and lysosomal enzyme release from, normal polymorphonuclear leukocytes and cells rendered incapable of ingesting particles by treatment with cytochalasin B. Neither phagocytosis nor lysosomal degranulation were prerequisites for enhanced O-.2 generation. (jci.org)
  • The three stimuli also enhanced O-.2 generation by normal (untreated) polymorphonuclear leukocytes, but only serum-treated zymosan and aggregated IgG were capable of provoking lysosomal enzyme release from normal cells. (jci.org)
  • Polymorphonuclear leukocytes, or PMNs, are a special family of white blood cells . (verywellhealth.com)
  • In order to assess the influence of poor diabetes control on function of leukocytes, polymorphonuclear leukocytes (PMN's) from patients with poorly controlled but nonketotic disease were studied before and after therapy. (diabetesjournals.org)
  • Leukocyte adhesion deficiency type 1 is a disorder that causes the immune system to malfunction, resulting in a form of immunodeficiency. (medlineplus.gov)
  • Starting from birth, people with leukocyte adhesion deficiency type 1 develop serious bacterial and fungal infections. (medlineplus.gov)
  • One of the first signs of leukocyte adhesion deficiency type 1 is a delay in the detachment of the umbilical cord stump after birth. (medlineplus.gov)
  • but, in infants with leukocyte adhesion deficiency type 1, this separation usually occurs at three weeks or later. (medlineplus.gov)
  • In leukocyte adhesion deficiency type 1, bacterial and fungal infections most commonly occur on the skin and mucous membranes such as the moist lining of the nose and mouth. (medlineplus.gov)
  • A hallmark of leukocyte adhesion deficiency type 1 is the lack of pus formation at the sites of infection. (medlineplus.gov)
  • Life expectancy in individuals with leukocyte adhesion deficiency type 1 is often severely shortened. (medlineplus.gov)
  • Leukocyte adhesion deficiency type 1 is estimated to occur in 1 per million people worldwide. (medlineplus.gov)
  • Mutations in the ITGB2 gene cause leukocyte adhesion deficiency type 1. (medlineplus.gov)
  • ITGB2 gene mutations that cause leukocyte adhesion deficiency type 1 lead to the production of a β2 subunit that cannot bind with other subunits to form β2 integrins. (medlineplus.gov)
  • Cox DP, Weathers DR. Leukocyte adhesion deficiency type 1: an important consideration in the clinical differential diagnosis of prepubertal periodontitis. (medlineplus.gov)
  • Harris ES, Weyrich AS, Zimmerman GA. Lessons from rare maladies: leukocyte adhesion deficiency syndromes. (medlineplus.gov)
  • Leukocyte adhesion deficiency (LAD) is a rare primary immunodeficiency. (medscape.com)
  • Thus the infections in patients with leukocyte adhesion deficiency act similarly as those observed in patients with neutropenia. (medscape.com)
  • Labial ulceration from which Escherichia coli was cultured in an 8-month-old girl with leukocyte adhesion deficiency type 1 (LAD I). Note the thin bluish scar at the superior aspect of the labia from an earlier cellulitis. (medscape.com)
  • This 3-year-old girl had leukocyte adhesion deficiency type I (LAD I) with complete absence of CD18 expression. (medscape.com)
  • This 10-month-old patient with severe leukocyte adhesion deficiency type I (LAD I) developed a cervical adenitis caused by Klebsiella pneumoniae. (medscape.com)
  • Leukocyte adhesion deficiency type I (LAD I) is a failure to express CD18, which composes the common ß 2 subunit of LFA1 family (ß2 integrins). (medscape.com)
  • In milder forms of leukocyte adhesion deficiency I (1-30% expression of CD8), patients may survive to adulthood. (medscape.com)
  • Patients with leukocyte adhesion deficiency II manifest the Bombay phenotype (ie, negative for O and H blood group antigens with potential production of anti-H antibody). (medscape.com)
  • However, IgM and IgG serum levels are within the reference range in patients with leukocyte adhesion deficiency II. (medscape.com)
  • Leukocyte adhesion deficiency II may be classified as one of the congenital disorders of glycosylation (CDG), a rapidly expanding group of metabolic syndromes with a wide symptomatology and severity. (medscape.com)
  • Currently, 18 subtypes have been reported: 12 are type I (dysfunctional lipid-linked oligosaccharide precursor synthesis), and 6 are type II (dysfunctional trimming/processing of the protein-bound oligosaccharide), including leukocyte adhesion deficiency II (CDG-IIc). (medscape.com)
  • Variants of leukocyte adhesion deficiency have also been reported, including fully expressed but nonfunctional CD18 and an E selectin that is expressed but rapidly cleaved from the cell surface (only present in soluble form). (medscape.com)
  • Another reported type of leukocyte adhesion deficiency involves dysfunction in platelet aggregation in addition to a defect in leukocyte adhesion. (medscape.com)
  • Thus, patients with this type of leukocyte adhesion deficiency manifest both severe bacterial infections and bleeding disorder. (medscape.com)
  • This leukocyte adhesion deficiency variant is associated with defective expression of the Rap-1 activator CalDAG-GEFI. (medscape.com)
  • Leukocyte adhesion deficiency (LAD) is a primary immunodeficiency that causes individuals to be abnormally susceptible to developing frequent soft-tissue infections, gum inflammation, and tooth loss. (primaryimmune.org)
  • Leukocyte adhesion deficiency type II (LAD2): Infants with LAD2 develop recurrent bacterial infections. (primaryimmune.org)
  • Leukocyte adhesion deficiency type III (LAD3): Individuals with LAD3 have recurrent bacterial infections that follow a similar course of infection as seen in individuals with LAD1. (primaryimmune.org)
  • Haploidentical in utero hematopoietic cell transplantation improves phenotype and can induce tolerance for postnatal same-donor transplants in the canine leukocyte adhesion deficiency. (wordnik.com)
  • Leukocytes are white blood cells such as granulocytes, monocytes and lymphocytes. (livestrong.com)
  • In order to asuure myself that my platelet preps aren't contaminated with RNA from leukocytes I am looking for a molecule expressed on monocytes, lymphocytes and PMN's that is not expressed in platelets that I could use as a negative control. (bio.net)
  • Lymphocyte - Lymphocytes make up 30% to 40% of all leukocytes. (howstuffworks.com)
  • Chemokines are now known to also function as regulatory molecules in leukocyte maturation, traffic and homing of lymphocytes, and the development of lymphoid tissues. (nih.gov)
  • The leukocyte count is normal or low, with relative increase of lymphocytes. (thesaurus.com)
  • The doctor may carry out a dipstick test, in which a chemical strip detects an enzyme called leukocyte esterase that points to the presence of white blood cells, typically related to an infection. (medicalnewstoday.com)
  • A quick way to detect white blood cells in urine is to look for leukocyte esterase, an enzyme found in the white blood cells. (ehow.co.uk)
  • Leukocyte esterase (LE) is an esterase (a type of enzyme) produced by leukocytes (white blood cells). (wikipedia.org)
  • Generally, white blood cells contain the enzyme leukocyte esterase, notes the American Association for Clinical Chemistry. (reference.com)
  • Finally, data on the arylsulfatase B activity (the deficient enzyme in MPS VI) in leukocytes, showed that one hour after completion of galsulfase infusion, enzyme activity is increased nearly eightfold but that the CS content in leucocytes remains more than 12-fold above basal level. (globenewswire.com)
  • An unusually high number of leukocytes in the urine indicates inflammation or infection along the urinary tract, often in the bladder or kidney. (medicalnewstoday.com)
  • Upon detection of inflammation, leukocytes initiate a highly orchestrated sequence of events, whereby they attach and roll along on the surface of the endothelium before squeezing through to the underlying tissue. (sciencemag.org)
  • A urinalysis is the definitive way to diagnose leukocytes and inflammation in the urinary tract. (livestrong.com)
  • The velocity of rolling leukocytes provides important information about the inflammation process, which aids biomedical researchers in the development of anti-inflammatory medications. (virginia.edu)
  • Over the past ten years, numerous chemokines have been identified as attractants of different types of blood leukocytes to sites of infection and inflammation. (nih.gov)
  • Leukocyte migaration from the blood into tissues is vital for immune surveillance and inflammation. (genome.jp)
  • and B. Contacting said plasma membrane lipid-associated Human Leukocyte Elastase (HLE), with said peptide antagonist under binding conditions so as to effect interaction of said antagonist and said plasma membrane lipid-associated Human Leukocyte Elastase (HLE), and thereby suppression of plasma membrane response to inflammation. (freepatentsonline.com)
  • Phagocyte recognition, uptake, and nonphlogistic degradation of neutrophils and other leukocytes undergoing apoptosis promote the resolution of inflammation. (jimmunol.org)
  • Although there is strong evidence that leukocytes can mediate tissue injury in inflammatory disorders 1 , little is known of the mechanisms that promote the resolution of inflammation by eliminating leukocytes from tissues. (jimmunol.org)
  • From Horwitz Adhesion molecule s involved in leukocyte emigration from vessels during inflammation 2. (picsearch.com)
  • Implicated in Note Entity Recruiting blood leukocytes to the site of inflammation Note E-selectin. (picsearch.com)
  • En inflammation kan också orsakas av till exempel slag eller brännskador. (lu.se)
  • Det finns en mängd olika vita blodkroppar, som cirkulerar i blodet, och de som först tar sig till en inflammation är neutrofilerna. (lu.se)
  • I Figur A visas en schematisk bild över händelseförloppet vid en inflammation som är kroppens försvar mot en. (lu.se)
  • Leukocytes in urine: A sign of urinary tract infection? (medicalnewstoday.com)
  • What do leukocytes in the urine mean? (medicalnewstoday.com)
  • Urine samples can sometimes contain leukocytes. (medicalnewstoday.com)
  • Having leukocytes in the urine may be a symptom of an infection. (medicalnewstoday.com)
  • Leukocytes can sometimes show in urine tests. (medicalnewstoday.com)
  • An absence of leukocyte esterase in the urine means that the urine is not likely to contain white blood cells, so it is not likely to be carrying infectious agents. (medicalnewstoday.com)
  • Having leukocytes in the urine without nitrites can also lead to a false-positive result that points to a bacterial infection when there is none. (medicalnewstoday.com)
  • The genital canal can sometimes pass leukocytes into the urine during the process of giving a sample. (medicalnewstoday.com)
  • So the laboratory may report leukocytes in urine without the patient really having an infection. (ehow.co.uk)
  • Leukocyte esterase is a urine test to look for white blood cells and other signs of infection. (medlineplus.gov)
  • Leukocyte esterase is a screening test used to detect a substance that suggests there are white blood cells in the urine. (medlineplus.gov)
  • A urine dipstick test can show leukocyte esterase. (livestrong.com)
  • If an excess of these leukocytes are found in urine, it can be a sign that there may be an infection present. (livestrong.com)
  • There are several symptoms to watch for if you suspect that your child may have an infection or leukocytes in his urine. (livestrong.com)
  • MayoClinic.com states that leukocyte esterase and nitrates are both produced because of an infection and, if found in urine, they are commonly signs of a urinary tract infection. (livestrong.com)
  • Treatment for children who have high leukocyte esterase in the urine can begin immediately. (livestrong.com)
  • A doctor needs to evaluate your child if you think she may have leukocytes in her urine. (livestrong.com)
  • A leukocyte esterase test (LE test) is a urine test for the presence of white blood cells and other abnormalities associated with infection. (wikipedia.org)
  • A urine sample that tests positive for both nitrite and leukocyte esterase should be cultured for pathogenic bacteria. (wikipedia.org)
  • It has been proposed that the reagent strip for leukocyte esterase designed for the testing of urine (Combur test UX) could be a useful tool for diagnosing spontaneous bacterial peritonitis SBP. (wikipedia.org)
  • The leukocyte esterase test is done using a color-sensitive dipstick that changes color to indicate the presence of white blood cells in urine, explains MedlinePlus. (reference.com)
  • A high white blood cell count in urine can indicate an inflamed kidney or urinary tract, which produce positive results in leukocyte esterase testing. (reference.com)
  • Laboratories testing patients' urine for leukocyte esterase use test strips and color changes on test pads to estimate the amount of protein in the urine, explains the American Association for Clinical Chemistry. (reference.com)
  • Leukocytes in urine but no infection. (rutgers.edu)
  • Like velcro, carbohydrate ligands on the circulating leukocytes bind to selectin molecules on the inner wall of the vessel, with marginal affinity . (wikipedia.org)
  • At the same time, chemokines released by macrophages activate the rolling leukocytes and cause surface integrin molecules to switch from the default low-affinity state to a high-affinity state. (wikipedia.org)
  • There are disclosed therapeutic compositions and methods using isolated nucleic acid molecules encoding a human chemokine beta-11 (Ck beta-11) polypeptide and a human leukocyte adhesion inhibitor-1 (LAI-1) polypeptide (previously termed chemokine α1(CKα1 or ckα-1), as well as Ck beta-11 and/or LAI-1. (google.com)
  • There are disclosed therapeutic compositions and methods using isolated nucleic acid molecules encoding a human chemokine beta-11 (Ck beta-11) polypeptide and a human leukocyte adhesion inhibitor-1 (LAI-1) polypeptide (previously termed chemokine α1(CKα1 or ckα-1), as well as Ck beta-11 and/or LAI-1 polypeptides themselves, as are vectors, host cells and recombinant methods for producing the same. (google.com)
  • By analogy to the behavior of other interferons and a class of nucleotidyl transferases on the polynucleotide-agarose chromatography, we suggest that the human leukocyte interferon having the polynucleotide-binding site is in a possibly "native" conformation and the loss of affinity for polynucleotide results from a degradative alteration of the native molecules. (pnas.org)
  • During this diapedesis of leukocytes, the leukocytes bind to endothelial cell adhesion molecules (CAM) and then migrate across the vascular endothelium. (genome.jp)
  • HLAs, or human leukocyte antigens, are molecules involved in the immune response. (wordnik.com)
  • As in other respiratory infections, leukocyte recruitment to the respiratory system in people with COVID-19 is orchestrated by specific leukocyte trafficking molecules, and when uncontrolled and excessive it results in various pathological complications, both in the lungs and in other organs. (nature.com)
  • Leukocytes initially roll along the vessel wall with the help of cell adhesion molecules where they can be activated by chemokines on the vascular endothelium, leading to their arrest, and transmigration through the endothelial barrier to exit the bloodstream and enter underlying tissues [reviewed in ( 18 ) and illustrated in Figure 1 ]. (frontiersin.org)
  • The granular leukocytes are the neutrophils, the eosinophils and the basophils. (ehow.co.uk)
  • My CBC came back with high WBC of 14.1, high neutrophils of 9.6 and high leukocytes of 3.57. (doctorslounge.com)
  • Neutrophil , type of white blood cell (leukocyte) that is characterized histologically by its ability to be stained by neutral dyes and functionally by its role in mediating immune responses against infectious microorganisms. (britannica.com)
  • The most common leukocyte is the neutrophil, so called because its small granules do not stain very darkly. (ehow.co.uk)
  • Systemic administration of catecholamines, such as the sympathetic neurotransmitters adrenaline and noradrenaline, increases neutrophil numbers in the bloodstream but has different effects on other leukocyte populations. (frontiersin.org)
  • One area of medicine in which human leucocyte antigens (HLA) proved to be of great importance is transplantation. (thefreedictionary.com)
  • HLA genes express their gene products on the surface of white blood cells (hence the name 'human leukocyte antigen,' although HLA class I genes (see 'Class I region' below) are also expressed on all nucleated cells) and were originally recognized to contain the genes encoding 'tissue antigens' or 'tissue types. (uptodate.com)
  • Human leukocyte antigens (HLAs) present protein fragments (peptides) to immune cells. (nih.gov)
  • Human leukocyte antigen (HLA) , any of the numerous antigens (substances capable of stimulating an immune response) involved in the major histocompatibility complex (MHC) in humans. (britannica.com)
  • Which human leukocyte antigens are associated with increased risk for Stevens-Johnson syndrome (SJS)? (medscape.com)
  • Helicobacter pylori lipopolysaccharide hinders polymorphonuclear leucocyte apoptosis. (nih.gov)
  • Secretory leukocyte peptidase inhibitor expression and apoptosis effect in oral leukoplakia and oral squamous cell carcinoma. (nih.gov)
  • The promising interim results from this ongoing clinical trial indicate that single-agent GCS-100 induces apoptosis (programmed cell death) in patients' CLL cells, reduces leukocyte count in some patients and is generally well tolerated. (thefreedictionary.com)
  • However, more recent studies may suggest that not yet metabolized clozapine is taken up by leukocytes and transformed by oxidative processes to apoptosis-inducing metabolites. (biomedsearch.com)
  • Therefore, the present work was aimed at determining the effect of intake of sodium fluoride (NaF) as an apoptosis inducer in leukocytes of rats treated for eight weeks with 1 or 50 parts per million (ppm) NaF. (fluoridealert.org)
  • These results indicate that NaF intoxication can be an apoptosis inducer in rat leukocytes treated with the compound for eight weeks. (fluoridealert.org)
  • The easy separation of these two classes of human leukocyte interferon makes the purification procedures more rational and will facilitate the preparation of both subspecies to a high degree of molecular homogeneity. (pnas.org)
  • Read the side effects of Interferon Alfa-N3 -Human Leukocyte derived as described in the medical literature. (medindia.net)
  • Transmigration of the leukocyte occurs as PECAM proteins, found on the leukocyte and endothelial cell surfaces, interact and effectively pull the cell through the endothelium. (wikipedia.org)
  • Once through the endothelium, the leukocyte must penetrate the basement membrane . (wikipedia.org)
  • These gaps can form through interactions of the leukocytes with the endothelium, but also autonomously through endothelial mechanics. (wikipedia.org)
  • We conclude that these five cytotoxic effector leukocyte subsets comprise the marginal pool by a CD11a/CX3CR1-mediated attachment to the endothelium. (jimmunol.org)
  • An adhesion molecule, E-selectin, which is upregulated on interleukin-1 activated endothelium, was found to mediate rolling between leukocytes and endothelial cells in vivo. (lu.se)
  • The cytoskeletons of the leukocytes are reorganised in such a way that the leukocytes are spread out over the endothelial cells. (wikipedia.org)
  • In this form, leukocytes extend pseudopodia and pass through gaps between endothelial cells. (wikipedia.org)
  • These 2 members mediate leukocyte adhesions to endothelial cells but they also serve as receptors for iC3b (inactivated C3b). (medscape.com)
  • This disease is a defect in fucose metabolism (lack of fucosylation of the carbohydrate selectin ligands) that results in failure to express the ligand for E and P selectin, sialyl Lewis-X (CD15s) expressed on leukocytes and endothelial cells. (medscape.com)
  • A leukocyte adherent to CAMs on the endothelial cells moves forward by leading-edge protrusion and retraction of its tail. (genome.jp)
  • Immediate demargination of leukocytes from vascular endothelial cells is promoted by sympathetic nervous system (SNS) catecholaminergic activity stimulating PBMCs that express predominantly high-affinity β2-adrenoceptors ( 1 , 3 , 6 - 10 ). (jimmunol.org)
  • Normal leukocyte esterase levels are negative, according to MedlinePlus. (reference.com)
  • A positive leukocyte screening test determines the possible existence of a urinary tract infection, according to MedlinePlus. (reference.com)
  • The human leukocyte antigen ( HLA ) system or complex is a gene complex encoding the major histocompatibility complex (MHC) proteins in humans. (wikipedia.org)
  • This invention relates to the storage of human white cells or leukocytes and pertains more specifically to a composition containing human plasma, modified fluid gelatin, and non-toxic buffer, into which leukocytes can be dispersed and stably maintained during storage at low temperature for periods of at least 24 hours, usually at 48 hours, and even up to two weeks or more. (google.com)
  • The Innate Immunity Signal Transduction in Human Leukocytes is a research study to determine the response of immune cells from the bloodstream. (nih.gov)
  • The human leukocyte antigen (HLA) complex is synonymous with the human MHC. (uptodate.com)
  • The major histocompatibility complex (MHC) in humans refers to a genetic region containing hundreds of genes, including the human leukocyte antigen (HLA) genes ( figure 1 ). (uptodate.com)
  • Human immunodeficiency virus type-1 Tat protein induces secretory leukocyte protease inhibitor expression in African green monkey but not human cells. (nih.gov)
  • Human leukocyte antigen (HLA) testing is also called HLA typing or tissue typing. (cancer.ca)
  • Complement and immunoglobulins stimulate superoxide production by human leukocytes independently of phagocytosis. (jci.org)
  • A method for modulation of plasma membrane associated Human Leukocyte Elastase (HLE) to inflammatory states by interaction of HLE with an antagonist to inhibit HLE and thereby interruption in plasma associated events (e.g. (freepatentsonline.com)
  • It has been previously reported that human leukocyte elastase (HLE) is involved in plasma membrane events during stimulation of immune cells, (Bristow and Flood, T Cell Antigen Immune Complexes Demonstrating Biologic & Proteolytic Activity , Int. Immunol. (freepatentsonline.com)
  • HLE is localized on the plasma membrane early in ontogeny and is granule-localized later in ontogeny, suggesting that HLE is an early differentiation marker (Borregaard and Cowland, Granules Of The Human Neutrophilic Polymorphonuclear Leukocyte , Blood, 89:3503-3521 ( 1997 ). (freepatentsonline.com)
  • In this study, we analyzed in human blood adhesion molecule and chemokine receptor profiles in 14 leukocyte subsets, and responsiveness of subsets to epinephrine in vivo and in vitro. (jimmunol.org)
  • A cobalamin-dependent N5-methyltetra-hydrofolate-homocysteine methyltransferase (methyl-transferase) was demonstrated in unfractioned extracts of human normal and leukemia leukocytes. (jci.org)
  • An association between Human Leukocyte Histocompatibility Antigen B-27 (HLA-B27) and major arthritis syndromes such as Ankylosing Spondylitis and Reactive Arthritis is well documented (Khan et al. (cdc.gov)
  • Agar Leukocyte Conditioned Medium (Agar-LCM) is a source of colony-stimulating factors for assays of hematopoietic progenitor cells from light-density fractions of human bone marrow and peripheral blood. (stemcell.com)
  • Agar-LCM is prepared using normal human peripheral blood leukocytes in an agar-containing medium. (stemcell.com)
  • Purification and properties of acid ribonucleases in human serum and leukocytes. (biomedsearch.com)
  • From normal human leukocytes, acid RNase was purified about 400-fold by the same procedure described previously except that rechromatography on Sephadex G-75 was omitted. (biomedsearch.com)
  • According to Medline Plus, an excess of both protein or vitamin C can lead to a negative test, even when there are leukocytes present. (livestrong.com)
  • 6 g/dL, total leukocyte count 20,300/dL, platelet count 392,000/dL, erythrocyte sedimentation rate (ESR) 84 mm in first hour, and C-reactive-protein 128 mg/L. (thefreedictionary.com)
  • Ex vivo stimulation with LPS of circulating leukocytes showed reduced production of CCL4, CXCL2, CXCL10 and TNF alpha protein in MMAW-SS treated rats. (cdc.gov)
  • female, suprapubic catheter, no stones, no bateria (C&S), recent scan of kidneys all clear BUT persistent leukocytes (sometimes with blood and protein, sometimes without). (rutgers.edu)
  • The white blood cells, or leukocytes, lack a protein on their surface that makes them unable to enter infection sites and kill bacteria and other foreign invaders. (primaryimmune.org)
  • White blood cells (leukocytes) perform most of their functions in tissues. (wikipedia.org)
  • Leukocytes use the blood as a transport medium to reach the tissues of the body. (wikipedia.org)
  • The recruitment of leukocytes from the blood is a prerequisite for their participation in inflammatory responses within the tissues. (springer.com)
  • These results indicate that necrotic cells can recruit leukocytes in a manner similar to cells in damaged tissues and suggest that this pathway could operate in different types of inflammatory events. (sciencemag.org)
  • They are produced locally in the tissues and act on leukocytes through selective receptors. (nih.gov)
  • Leukocytes migrate through the body by shuttling between the vascular system and tissues. (frontiersin.org)
  • 2 3 In addition, as telomere length within individuals is generally strongly correlated across tissue types, leucocyte based measures might also serve as a marker of telomere length in less accessible tissues. (bmj.com)
  • Create healthcare diagrams like this example called Leukocytes - Granulocytes in minutes with SmartDraw. (smartdraw.com)
  • Granulocytes - Granulocytes make up 50% to 60% of all leukocytes. (howstuffworks.com)
  • For this reason, PMNs are also frequently called "granular leukocytes" or granulocytes. (verywellhealth.com)
  • The doctors had said the internal bleeding has been brought under control and her blood platelet and leukocyte count also improved after administering plasma and platelets. (thefreedictionary.com)
  • This article concentrates on recent evidence that interactions between leukocyte-derived opioid peptides and their receptors on peripheral sensory neurons can result in potent, clinically relevant inhibition of pathological pain. (nih.gov)
  • Leukocyte membrane receptors for complement and immunoglobulins may therefore not only serve in particle recognition but also may initiate biochemical events which accompany phagocytosis and killing. (jci.org)
  • Despite all these findings and that chemokines are main regulators of leukocyte traffic, the role of chemokine receptors in SNS-induced demargination of leukocytes has attracted surprisingly little attention ( 22 , 24 , 29 - 31 ). (jimmunol.org)
  • This process forms part of the innate immune response , involving the recruitment of non-specific leukocytes. (wikipedia.org)
  • It has been demonstrated that leukocyte recruitment is halted whenever any of these steps is suppressed. (wikipedia.org)
  • When amputated zebrafish tails were bathed in isotonic conditions that would not induce cell swelling, the presence of dead HeLa cells in the solution triggered the recruitment of leukocytes to injured tails. (sciencemag.org)
  • The ability of the dead HeLa cells to stimulate leukocyte recruitment required the cells to have swollen nuclei and for cPLA 2 to be present. (sciencemag.org)
  • [ 1 , 2 ] The clinical picture is characterized by marked leukocytosis and localized bacterial infections that are difficult to detect until they have progressed to an extensive level secondary to lack of leukocyte recruitment at the site of infection. (medscape.com)
  • This process can be understood in several steps: Chemoattraction Rolling adhesion Tight adhesion (Endothelial) Transmigration It has been demonstrated that leukocyte recruitment is halted whenever any of these steps is suppressed. (wikipedia.org)
  • Aside from β2-adrenoceptor expression, the selective recruitment of leukocytes is determined by the specific profile of adhesion molecule expression on the cells. (jimmunol.org)
  • Because of the delicate air-blood barrier for gas exchange, resident immune cells in the lung maintain a very fine balance between protection and disease as they must constantly clear inhaled air particles and rapidly respond to pathogens through the highly coordinated recruitment of specific innate and adaptive leukocytes, which is critical for pathogen clearance. (nature.com)
  • Circulating leukocytes are localised towards the site of injury or infection due to the presence of chemokines. (wikipedia.org)
  • Here is a brief summary of each of the four steps currently thought to be involved in leukocyte extravasation: Upon recognition of and activation by pathogens, resident macrophages in the affected tissue release cytokines such as IL-1, TNFα and chemokines. (wikipedia.org)
  • Upon stressful stimuli or in response to releasing agents such as corticotropin-releasing factor, cytokines, chemokines and catecholamines, leukocytes secrete opioids. (nih.gov)
  • These images are a random sampling from a Bing search on the term "CSF Leukocyte. (fpnotebook.com)
  • His initial complete blood count showed the following results: hemoglobin 14.4 g/dL, hematocrit 52%, leucocyte count 8900/mm3, and platelet count 415000/mm3. (thefreedictionary.com)
  • These factors include the progression of cancer, tumour location (genital), anemia, a high platelet count, a high leukocyte count (450 x 10[sup. (thefreedictionary.com)
  • The aim of this study was to evaluate the molecular changes and responsiveness of circulating leukocytes following welding fume exposure. (cdc.gov)
  • Here, we review the molecular signals orchestrating leukocyte trafficking to airway and lung compartments during primary pneumotropic influenza virus infections and discuss potential similarities to distinct courses of primary SARS-CoV-2 infections. (nature.com)
  • found that a leukocyte count greater than 15,000/mm3 and a serum glucose greater than 150 mg./dl. (wordnik.com)
  • Other pertinent laboratory parameters to monitor are serum glucose and leukocyte count. (wordnik.com)
  • The level of vitamin C in leukocytes more accurately correlates to tissue stores compared with serum levels, because these cells are not affected acutely by circadian rhythm or dietary changes. (medscape.com)
  • It is found in various secretions including seminal plasma, cervical mucus, and bronchial secretions, and has affinity for trypsin, leukocyte elastase, and cathepsin G. Its inhibitory effect contributes to the immune response by protecting epithelial surfaces from attack by endogenous proteolytic enzymes. (nih.gov)
  • Chemotaxis experiments will be performed in order to ascertain the migratory characteristics of leukocytes towards specific chemoattractants. (clinicaltrials.gov)
  • Leukocyte extravasation , less commonly called diapedesis , is the movement of leukocytes out of the circulatory system and towards the site of tissue damage or infection. (wikipedia.org)
  • Leukocyte extravasation occurs mainly in post-capillary venules , where haemodynamic shear forces are minimised. (wikipedia.org)
  • Leukocyte extravasation (also commonly known as leukocyte adhesion cascade or diapedesis - the passage of cells through the intact vessel wall) is the movement of leukocytes out of the circulatory system and towards the site of tissue damage or infection. (wikipedia.org)
  • In general, leukocytes are involved in the defense of an organism and protect it from disease by promoting or inhibiting inflammatory responses. (wikipedia.org)
  • These results showed a reduced capacity of circulating leukocytes to produce inflammatory proteins in response to a secondary stimulus following a metal-rich particulate matter pulmonary exposure and provide mechanistic insight into epidemiological and experimental evidence illustrating immunosuppression following welding fume exposure. (cdc.gov)
  • It is accepted that inflammatory mediators released from leukocytes contribute to the generation of pain. (nih.gov)
  • This study assessed the effects of anti-inflammatory glucocorticoids on this leukocyte clearance mechanism. (jimmunol.org)
  • We conclude that potentiation of nonphlogistic clearance of apoptotic leukocytes by phagocytes is a hitherto unrecognized property of glucocorticoids that has potential implications for therapies aimed at promoting the resolution of inflammatory diseases. (jimmunol.org)
  • We also discuss how an imbalance in vascular activation by leukocytes outside the airways and lungs may contribute to extrapulmonary inflammatory complications in subsets of patients with COVID-19. (nature.com)
  • This thesis describes early inflammatory responses involving interactions between leukocytes and the endothelial lining of the blood vessel wall. (lu.se)
  • Once in the interstitial fluid , leukocytes migrate along a chemotactic gradient towards the site of injury or infection. (wikipedia.org)
  • If the test for leukocyte esterase is positive but finds no nitrite, an infection may still be present. (medicalnewstoday.com)
  • Signaling through the β2 integrins triggers the transport of the attached leukocyte across the blood vessel wall to the site of infection or injury. (medlineplus.gov)
  • Immune cells and their response are responsible for giving protection from pathogen invasion, adverse disruption of the number and activity of leucocyte were considered to be one of the fundamental notions for thalassemia patients to have an altered immune response, therefore susceptible for infection (7, 8). (thefreedictionary.com)
  • In children, this increase in leukocytes is most likely due to a urinary tract infection. (livestrong.com)
  • Nuclear swelling triggers the production of chemoattractants to recruit leukocytes to injured or dead tissue. (sciencemag.org)
  • M. Saraswat, K. V. Arya and H. Sharma, "Leukocyte Segmentation in Tissue Images Using Differential Evolution Algorithm," Swarm and Evolutionary Computation, Vol. 11, 2013, pp. 46-54. (scirp.org)
  • When the tissue demand is intense, marrow production and release may accelerate dramatically, resulting in a left shift and toxic changes (see Leukocyte Disorders:Numerical Abnormalities ). (merckvetmanual.com)
  • The aim of this study is to investigate the mechanisms whereby leukocytes are recruited to the lung in chronic obstructive pulmonary disease (COPD) and cause tissue destruction. (clinicaltrials.gov)
  • The hypothesis is that in COPD more leukocytes enter the lung and it is these cells that are responsible for the degradation of lung tissue. (clinicaltrials.gov)
  • Conclusion Available observational data show an inverse association between leucocyte telomere length and risk of coronary heart disease independent of conventional vascular risk factors. (bmj.com)
  • The major source of local endogenous opioid ligands (beta-endorphin, enkephalins, endomorphins and dynorphin) are leukocytes. (nih.gov)
  • When we become infected, our bodies produce a chemical called leukocyte endogenous mediator LEM, which decreases the amount of iron in our bloodstream. (wordnik.com)
  • For many immunological and cell culture workflows, the first step is to specifically isolate leukocytes from the blood. (genengnews.com)
  • We, the researchers at Imperial College London, will isolate leukocytes from the blood of patients with COPD, healthy smokers and normal subjects and measure the movement of the leukocytes to chemoattractants. (clinicaltrials.gov)
  • Expression of p53, bcl-2, and caspade-3 were used as apoptotic and general metabolism indicators of leukocyte-like indicators of the (INT) oxidation system. (fluoridealert.org)
  • Expression of p53, bcl-2, and caspase-3 were determined in leukocytes by Western blot, and general metabolism of leukocytes was analyzed with a commercial kit. (fluoridealert.org)
  • A leukocyte is a white blood cell, vital to the defenses of the immune system against disease. (medicalnewstoday.com)
  • Daix (France), February 26, 2018 - Inventiva, a biopharmaceutical company developing innovative therapies in nonalcoholic steatohepatitis (NASH), systemic sclerosis (SSc) and mucopolysaccharidosis (MPS), today announced the positive outcomes of a biomarker study to evaluate intracellular glycosaminoglycans (GAGs) levels in leukocytes as a disease activity biomarker in MPS VI. (globenewswire.com)
  • Objective To assess the association between leucocyte telomere length and risk of cardiovascular disease. (bmj.com)
  • In a comparison of the shortest versus longest third of leucocyte telomere length, the pooled relative risk for coronary heart disease was 1.54 (95% confidence interval 1.30 to 1.83) in all studies, 1.40 (1.15 to 1.70) in prospective studies, and 1.80 (1.32 to 2.44) in retrospective studies. (bmj.com)
  • The leukocyte application detects and tracks rolling leukocytes (white blood cells) in in vivo video microscopy of blood vessels. (virginia.edu)
  • The expression and clinical significance of secretory leukocyte proteinase inhibitor (SLPI) in mammary carcinoma using bioinformatics analysis. (nih.gov)
  • The decrease in glucose concentration was directly proportional to both the leukocyte count and preanalytical time, confirming unusually high leukocyte-mediated in vitro glucose consumption (3, 4). (thefreedictionary.com)
  • Properties of leukocytes enzymes resembled those of methyltransferases previously studied in bacteria and other animal cells. (jci.org)
  • For example, the carbohydrate ligand for P-selectin, P-selectin glycoprotein ligand-1 (PSGL-1), is expressed by different types of leukocytes (white blood cells). (wikipedia.org)
  • White blood cells, or leukocytes, make up one of the three types of blood cell. (ehow.co.uk)
  • Also called leukocyte , white cell , white corpuscle . (thefreedictionary.com)
  • All white blood cells are known officially as leukocytes . (howstuffworks.com)
  • What levels (counts) of leukocytes (White Blood Cells-WBC) have you experienced? (rutgers.edu)