Poly A-U: A double-stranded polyribonucleotide comprising polyadenylic and polyuridylic acids.Poly dA-dT: Polydeoxyribonucleotides made up of deoxyadenine nucleotides and thymine nucleotides. Present in DNA preparations isolated from crab species. Synthetic preparations have been used extensively in the study of DNA.Polydeoxyribonucleotides: A group of 13 or more deoxyribonucleotides in which the phosphate residues of each deoxyribonucleotide act as bridges in forming diester linkages between the deoxyribose moieties.Poly A: A group of adenine ribonucleotides in which the phosphate residues of each adenine ribonucleotide act as bridges in forming diester linkages between the ribose moieties.Poly(ADP-ribose) Polymerases: Enzymes that catalyze the transfer of multiple ADP-RIBOSE groups from nicotinamide-adenine dinucleotide (NAD) onto protein targets, thus building up a linear or branched homopolymer of repeating ADP-ribose units i.e., POLY ADENOSINE DIPHOSPHATE RIBOSE.Poly C: A group of cytosine ribonucleotides in which the phosphate residues of each cytosine ribonucleotide act as bridges in forming diester linkages between the ribose moieties.Poly U: A group of uridine ribonucleotides in which the phosphate residues of each uridine ribonucleotide act as bridges in forming diester linkages between the ribose moieties.Poly I-C: Interferon inducer consisting of a synthetic, mismatched double-stranded RNA. The polymer is made of one strand each of polyinosinic acid and polycytidylic acid.Dopamine: One of the catecholamine NEUROTRANSMITTERS in the brain. It is derived from TYROSINE and is the precursor to NOREPINEPHRINE and EPINEPHRINE. Dopamine is a major transmitter in the extrapyramidal system of the brain, and important in regulating movement. A family of receptors (RECEPTORS, DOPAMINE) mediate its action.Poly(A)-Binding Proteins: Proteins that bind to the 3' polyadenylated region of MRNA. When complexed with RNA the proteins serve an array of functions such as stabilizing the 3' end of RNA, promoting poly(A) synthesis and stimulating mRNA translation.Poly G: A group of guanine ribonucleotides in which the phosphate residues of each guanine ribonucleotide act as bridges in forming diester linkages between the ribose moieties.Poly T: A group of thymine nucleotides in which the phosphate residues of each thymine nucleotide act as bridges in forming diester linkages between the deoxyribose moieties.Poly Adenosine Diphosphate Ribose: A polynucleotide formed from the ADP-RIBOSE moiety of nicotinamide-adenine dinucleotide (NAD) by POLY(ADP-RIBOSE) POLYMERASES.Poly I: A group of inosine ribonucleotides in which the phosphate residues of each inosine ribonucleotide act as bridges in forming diester linkages between the ribose moieties.Length of Stay: The period of confinement of a patient to a hospital or other health facility.Molecular Sequence Data: Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.Base Sequence: The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.Amino Acid Sequence: The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.Polyribonucleotides: A group of 13 or more ribonucleotides in which the phosphate residues of each ribonucleotide act as bridges in forming diester linkages between the ribose moieties.Dopamine Antagonists: Drugs that bind to but do not activate DOPAMINE RECEPTORS, thereby blocking the actions of dopamine or exogenous agonists. Many drugs used in the treatment of psychotic disorders (ANTIPSYCHOTIC AGENTS) are dopamine antagonists, although their therapeutic effects may be due to long-term adjustments of the brain rather than to the acute effects of blocking dopamine receptors. Dopamine antagonists have been used for several other clinical purposes including as ANTIEMETICS, in the treatment of Tourette syndrome, and for hiccup. Dopamine receptor blockade is associated with NEUROLEPTIC MALIGNANT SYNDROME.Polymorphism, Restriction Fragment Length: Variation occurring within a species in the presence or length of DNA fragment generated by a specific endonuclease at a specific site in the genome. Such variations are generated by mutations that create or abolish recognition sites for these enzymes or change the length of the fragment.3,4-Dihydroxyphenylacetic Acid: A deaminated metabolite of LEVODOPA.Poly(A)-Binding Protein I: A poly(A) binding protein that has a variety of functions such as mRNA stabilization and protection of RNA from nuclease activity. Although poly(A) binding protein I is considered a major cytoplasmic RNA-binding protein it is also found in the CELL NUCLEUS and may be involved in transport of mRNP particles.Polynucleotide Adenylyltransferase: An enzyme that catalyzes the synthesis of polyadenylic acid from ATP. May be due to the action of RNA polymerase (EC 18.104.22.168) or polynucleotide adenylyltransferase (EC 22.214.171.124). EC 126.96.36.199.Dopamine Plasma Membrane Transport Proteins: Sodium chloride-dependent neurotransmitter symporters located primarily on the PLASMA MEMBRANE of dopaminergic neurons. They remove DOPAMINE from the EXTRACELLULAR SPACE by high affinity reuptake into PRESYNAPTIC TERMINALS and are the target of DOPAMINE UPTAKE INHIBITORS.Corpus Striatum: Striped GRAY MATTER and WHITE MATTER consisting of the NEOSTRIATUM and paleostriatum (GLOBUS PALLIDUS). It is located in front of and lateral to the THALAMUS in each cerebral hemisphere. The gray substance is made up of the CAUDATE NUCLEUS and the lentiform nucleus (the latter consisting of the GLOBUS PALLIDUS and PUTAMEN). The WHITE MATTER is the INTERNAL CAPSULE.Receptors, Dopamine D2: A subfamily of G-PROTEIN-COUPLED RECEPTORS that bind the neurotransmitter DOPAMINE and modulate its effects. D2-class receptor genes contain INTRONS, and the receptors inhibit ADENYLYL CYCLASES.DNA: A deoxyribonucleotide polymer that is the primary genetic material of all cells. Eukaryotic and prokaryotic organisms normally contain DNA in a double-stranded state, yet several important biological processes transiently involve single-stranded regions. DNA, which consists of a polysugar-phosphate backbone possessing projections of purines (adenine and guanine) and pyrimidines (thymine and cytosine), forms a double helix that is held together by hydrogen bonds between these purines and pyrimidines (adenine to thymine and guanine to cytosine).Dopamine Agonists: Drugs that bind to and activate dopamine receptors.Cloning, Molecular: The insertion of recombinant DNA molecules from prokaryotic and/or eukaryotic sources into a replicating vehicle, such as a plasmid or virus vector, and the introduction of the resultant hybrid molecules into recipient cells without altering the viability of those cells.RNA, Messenger: RNA sequences that serve as templates for protein synthesis. Bacterial mRNAs are generally primary transcripts in that they do not require post-transcriptional processing. Eukaryotic mRNA is synthesized in the nucleus and must be exported to the cytoplasm for translation. Most eukaryotic mRNAs have a sequence of polyadenylic acid at the 3' end, referred to as the poly(A) tail. The function of this tail is not known for certain, but it may play a role in the export of mature mRNA from the nucleus as well as in helping stabilize some mRNA molecules by retarding their degradation in the cytoplasm.Dopaminergic Neurons: Neurons whose primary neurotransmitter is DOPAMINE.Molecular Weight: The sum of the weight of all the atoms in a molecule.Kinetics: The rate dynamics in chemical or physical systems.PolynucleotidesNucleus Accumbens: Collection of pleomorphic cells in the caudal part of the anterior horn of the LATERAL VENTRICLE, in the region of the OLFACTORY TUBERCLE, lying between the head of the CAUDATE NUCLEUS and the ANTERIOR PERFORATED SUBSTANCE. It is part of the so-called VENTRAL STRIATUM, a composite structure considered part of the BASAL GANGLIA.Microdialysis: A technique for measuring extracellular concentrations of substances in tissues, usually in vivo, by means of a small probe equipped with a semipermeable membrane. Substances may also be introduced into the extracellular space through the membrane.Polyesters: Polymers of organic acids and alcohols, with ester linkages--usually polyethylene terephthalate; can be cured into hard plastic, films or tapes, or fibers which can be woven into fabrics, meshes or velours.Dopamine Agents: Any drugs that are used for their effects on dopamine receptors, on the life cycle of dopamine, or on the survival of dopaminergic neurons.Receptors, Dopamine: Cell-surface proteins that bind dopamine with high affinity and trigger intracellular changes influencing the behavior of cells.Ventral Tegmental Area: A region in the MESENCEPHALON which is dorsomedial to the SUBSTANTIA NIGRA and ventral to the RED NUCLEUS. The mesocortical and mesolimbic dopaminergic systems originate here, including an important projection to the NUCLEUS ACCUMBENS. Overactivity of the cells in this area has been suspected to contribute to the positive symptoms of SCHIZOPHRENIA.Substantia Nigra: The black substance in the ventral midbrain or the nucleus of cells containing the black substance. These cells produce DOPAMINE, an important neurotransmitter in regulation of the sensorimotor system and mood. The dark colored MELANIN is a by-product of dopamine synthesis.Nucleic Acid Conformation: The spatial arrangement of the atoms of a nucleic acid or polynucleotide that results in its characteristic 3-dimensional shape.Dopamine Uptake Inhibitors: Drugs that block the transport of DOPAMINE into axon terminals or into storage vesicles within terminals. Most of the ADRENERGIC UPTAKE INHIBITORS also inhibit dopamine uptake.Time Factors: Elements of limited time intervals, contributing to particular results or situations.Polymers: Compounds formed by the joining of smaller, usually repeating, units linked by covalent bonds. These compounds often form large macromolecules (e.g., BIOPOLYMERS; PLASTICS).Receptors, Dopamine D1: A subfamily of G-PROTEIN-COUPLED RECEPTORS that bind the neurotransmitter DOPAMINE and modulate its effects. D1-class receptor genes lack INTRONS, and the receptors stimulate ADENYLYL CYCLASES.Rats, Sprague-Dawley: A strain of albino rat used widely for experimental purposes because of its calmness and ease of handling. It was developed by the Sprague-Dawley Animal Company.Ductus Arteriosus: A fetal blood vessel connecting the pulmonary artery with the descending aorta.Mesencephalon: The middle of the three primitive cerebral vesicles of the embryonic brain. Without further subdivision, midbrain develops into a short, constricted portion connecting the PONS and the DIENCEPHALON. Midbrain contains two major parts, the dorsal TECTUM MESENCEPHALI and the ventral TEGMENTUM MESENCEPHALI, housing components of auditory, visual, and other sensorimoter systems.Tyrosine 3-Monooxygenase: An enzyme that catalyzes the conversion of L-tyrosine, tetrahydrobiopterin, and oxygen to 3,4-dihydroxy-L-phenylalanine, dihydrobiopterin, and water. EC 188.8.131.52.Escherichia coli: A species of gram-negative, facultatively anaerobic, rod-shaped bacteria (GRAM-NEGATIVE FACULTATIVELY ANAEROBIC RODS) commonly found in the lower part of the intestine of warm-blooded animals. It is usually nonpathogenic, but some strains are known to produce DIARRHEA and pyogenic infections. Pathogenic strains (virotypes) are classified by their specific pathogenic mechanisms such as toxins (ENTEROTOXIGENIC ESCHERICHIA COLI), etc.Poly(A)-Binding Protein II: A poly(A) binding protein that is involved in promoting the extension of the poly A tails of MRNA. The protein requires a minimum of ten ADENOSINE nucleotides in order for binding to mRNA. Once bound it works in conjunction with CLEAVAGE AND POLYADENYLATION SPECIFICITY FACTOR to stimulate the rate of poly A synthesis by POLY A POLYMERASE. Once poly-A tails reach around 250 nucleotides in length poly(A) binding protein II no longer stimulates POLYADENYLATION. Mutations within a GCG repeat region in the gene for poly(A) binding protein II have been shown to cause the disease MUSCULAR DYSTROPHY, OCULOPHARYNGEAL.Amphetamine: A powerful central nervous system stimulant and sympathomimetic. Amphetamine has multiple mechanisms of action including blocking uptake of adrenergics and dopamine, stimulation of release of monamines, and inhibiting monoamine oxidase. Amphetamine is also a drug of abuse and a psychotomimetic. The l- and the d,l-forms are included here. The l-form has less central nervous system activity but stronger cardiovascular effects. The d-form is DEXTROAMPHETAMINE.
Digital transcriptome subtraction
... such as poly-dT or poly-dA. Human repetitive DNA. Length - parameter is dependent on the optimized read length specific to the ... Messenger RNA is then purified using an oligo-dT column that binds to the poly-A tail, a signal specifically found on ... Minimum hit length for a positive match of human sequence is typically 30 consecutive identical bases, which equates to a BLAST ...
Helicos single molecule fluorescent sequencing
... dT)50. To be compatible with the oligo(dT)50 primer on the flow cell surface, it is necessary to generate a poly(dA) tail of at ... dA) tails 90 to 200 nucleotides long. To generate tails of this length, it is first necessary to estimate how many 3' ends ... dT) on the surface. Generation of a 3' poly(dA) tail can be accomplished with a variety of different ligases or polymerases. If ... samples for sequencing are prepared in such a way that the poly(A) tail is longer than the oligo(dT)50 on the surface of the ...
In addition to providing flexibility and length, the poly dA portion of the linker also allows further purification of the mRNA ... Barendt PA, Ng DT, McQuade CN, Sarkar CA (2013). "Streamlined Protocol for mRNA Display". ACS Combinatorial Science. 15 (2): 77 ... which usually consists of poly dA nucleotides (Figure 2). The ligated mRNA-DNA-puromycin library is translated in Red Nova ... This enables the efficient production of high-quality, full-length mRNA-polypeptide fusion. Rihe Liu et al. optimized the 3'- ...
Da Silva AP, Pollett A, Rittling SR, Denhardt DT, Sodek J, Zohar R (September 2006). "Exacerbated tissue destruction in DSS- ... Full-length OPN (OPN-FL) can be modified by thrombin cleavage, which exposes a cryptic sequence, SVVYGLR on the cleaved form of ... Contiguous stretches of high negative charge in OPN have been identified and named the polyAsp motif (poly-aspartic acid) and ... Koh A, da Silva AP, Bansal AK, Bansal M, Sun C, Lee H, Glogauer M, Sodek J, Zohar R (December 2007). "Role of osteopontin in ...
DT (26 June 2001). "Rescue of a telomere length defect of Nijmegen breakage syndrome cells requires NBS and telomerase ... Lajud, SA; Nagda, DA; Yamashita, T; Zheng, J; Tanaka, N; Abuzeid, WM; Civantos, A; Bezpalko, O; O'Malley BW, Jr; Li, D (15 ... When combined with treatment of PARP (poly (ADP-ribose) polymerase) inhibitor (known as PARPi), these cells showed an even ... Farmer, H; McCabe, N; Lord, CJ; Tutt, AN; Johnson, DA; Richardson, TB; Santarosa, M; Dillon, KJ; Hickson, I; Knights, C; Martin ...
Jaitin DA, Kenigsberg E, Keren-Shaul H, Elefant N, Paul F, Zaretsky I, Mildner A, Cohen N, Jung S, Tanay A, Amit I (February ... Lee JH, Daugharthy ER, Scheiman J, Kalhor R, Yang JL, Ferrante TC, Terry R, Jeanty SS, Li C, Amamoto R, Peters DT, Turczyk BM, ... Zhao W, He X, Hoadley KA, Parker JS, Hayes DN, Perou CM (June 2014). "Comparison of RNA-Seq by poly (A) capture, ribosomal RNA ... Identifying gene start sites is of use for promoter analysis and for the cloning of full-length cDNAs. SAGE and CAGE methods ...
Single cell sequencing
While this method successfully captures full-length total RNA transcripts for sequencing and detected a variety of non-poly(A) ... Montoro DT, Haber AL, Biton M, Vinarsky V, Lin B, Birket SE, et al. (August 2018). "A revised airway epithelial hierarchy ... Cao J, Packer JS, Ramani V, Cusanovich DA, Huynh C, Daza R, et al. (August 2017). "Comprehensive single-cell transcriptional ... MDA products result in a length of about 12 kb and ranges up to around 100 kb, enabling its use in DNA sequencing. In 2017 ...
Minimum spanning tree
Hence, the total time required for finding an optimal DT for all graphs with r vertices is: 2. (. r. 2. ). ⋅. r. 2. (. r. 2. + ... Steele, J. Michael (2002), "Minimal spanning trees for graphs with random edge lengths", Mathematics and computer science, II ( ... AssunÇão, R. M.; M. C. Neves; G. Câmara; C. Da Costa Freitas (2006). "Efficient regionalization techniques for socio‐economic ... Holm, Jacob; de Lichtenberg, Kristian; Thorup, Mikkel (2001), "Poly-logarithmic deterministic fully dynamic algorithms for ...
Minimum spanning tree
The runtime complexity of a DT is the largest number of queries required to find the MST, which is just the depth of the DT. A ... Currency is an acceptable unit for edge weight - there is no requirement for edge lengths to obey normal rules of geometry such ... AssunÇão, R. M.; M. C. Neves; G. Câmara; C. Da Costa Freitas (2006). "Efficient regionalization techniques for socio‐economic ... "Poly-logarithmic deterministic fully dynamic algorithms for connectivity, minimum spanning tree, 2-edge, and biconnectivity", ...
Interferencia de ARN, a enciclopedia libre
... silenciamento dos virus da hepatite A e da hepatite B, silenciamento da expresión xénica da influenza, e a ... Humphreys DT, Westman BJ, Martin DI, Preiss T (2005). "MicroRNAs control translation initiation by inhibiting eukaryotic ... initiation factor 4E/cap and poly(A) tail function.". Proc Natl Acad Sci USA 102 (47): 16961-16966. PMC 1287990. PMID 16287976 ... "Induction of the interferon response by siRNA is cell type- and duplex length-dependent". RNA 12 (6): 988-93. PMC 1464853 ...
Yang W, Dicker DT, Chen J, El-Deiry WS (March 2008). "CARPs enhance p53 turnover by degrading 14-3-3sigma and stabilizing MDM2 ... The full length p53 isoform proteins can be subdivided into different protein domains. Starting from the N-terminus, there are ... Proctor CJ, Gray DA (August 2008). "Explaining oscillations and variability in the p53-Mdm2 system". BMC Systems Biology. 2 (75 ... Malanga M, Pleschke JM, Kleczkowska HE, Althaus FR (May 1998). "Poly(ADP-ribose) binds to specific domains of p53 and alters ...
Ramsay DT, Kent JC, Hartmann RA, Hartmann PE (2005). "Anatomy of the Lactating Human Breast Redefined with Ultrasound Imaging ... Karanas, Y. L.; Leong, D. S.; Da Lio, A.; Waldron, K.; Watson, J. P.; Chang, H.; Shaw, W. W. (2003). "Surgical Treatment of ... Silicone-gel implants can be difficult to emplace with this incision, because of the short, five-centimetre length (~ 5.0 cm.) ... Polyethylene terephthalate), which was attached to the rear of the breast-implant shell. Second generation In the 1970s, ...
Irritable bowel syndrome
Drossman DA, Li Z, Andruzzi E, Temple RD, Talley NJ, Thompson WG, Whitehead WE, Janssens J, Funch-Jensen P, Corazziari E; Li; ... Thabane M, Kottachchi DT, Marshall JK; Kottachchi; Marshall (2007). "Systematic review and meta-analysis: The incidence and ... Among the osmotic laxatives, doses of 17-26 g/d of polyethylene glycol have been well studied. Lubiprostone (Amitiza) is a ... Correlation of bowel symptoms with colonic transit, length, and faecal load in functional faecal retention 2009;56:83-8 ...
Hughes AE, Bradley DT, Campbell M, Lechner J, Dash DP, Simpson DA, Willoughby CE (2011). "Mutation Altering the miR-184 Seed ... duplex about 22 nucleotides in length. Overall hairpin length and loop size influence the efficiency of Dicer processing. The ... a poly(A) tail), and spliced. Animal miRNAs are initially transcribed as part of one arm of an ∼80 nucleotide RNA stem-loop ... Chen C, Ridzon DA, Broomer AJ, Zhou Z, Lee DH, Nguyen JT, Barbisin M, Xu NL, Mahuvakar VR, Andersen MR, Lao KQ, Livak KJ, ...
Freedman DA, Wu L, Levine AJ (January 1999). "Functions of the MDM2 oncoprotein". Cellular and Molecular Life Sciences. 55 (1 ... The full-length transcript of the mdm2 gene encodes a protein of 491 amino acids with a predicted molecular weight of 56kDa. ... Yang W, Dicker DT, Chen J, El-Deiry WS (March 2008). "CARPs enhance p53 turnover by degrading 14-3-3sigma and stabilizing MDM2 ...
List of firearms
DP LMG DA (Soviet Union - Aircraft-Mounted Machine Gun - 7.62×54mmR) DPM (Soviet Union - Light Machine Gun - 7.62×54mmR) DT ( ... Poly Technologies Rifles Poly Technologies AK-47 (China, Soviet Union - Semi-Automatic Rifle - 7.62×39mm: AK-47 Variant) Poly ... Full length variant of the AK-100 series, chambered in 5.56×45mm NATO. This is the main rifle in the AK-100 series exported to ... Poly Technologies Legend AK-47S (China, Soviet Union - Semi-Automatic Rifle - 7.62×39mm: AK-47 Variant) Poly Technologies ...
... ce cf cg ch ci cj ck cl cm cn co cp cq cr cs ct cu cv cw cx cy cz da db dc dd de df dg dh di dj dk dl dm dn do dp dq dr ds dt ... which imparts a somewhat longer total length that the white-tailed eagle, and a longer mean tarsal length. ... Propagation of the Urals and adjacent territories to poly-terrestrial sources: 107-122. ... tail and tarsal length, or body mass rather than wingspan or total length. As expected for many widely distributed animals of ...
CiteSeerX - Upstream mononucleotide A-repeats play a cis-regulatory role in mammals through the DICER1 and Ago proteins
by varying poly(dA:dT) tract length and continuity (16). However, transcriptional regulation is a dynamic and competitive ... It is hypothesized that one intrinsic property of poly(dA:dT) is to resist sharp DNA bending (15). Thus, poly(dA:dT) tracts ... It is hypothesized that one intrinsic property of poly(dA:dT) is to resist sharp DNA bending =-=(15)-=-. Thus, poly(dA:dT) ... A recent study showed that gene transcription can be fine-tuned by varying poly(dA:dT) tract length and continuity =-=(16)-=-. ...
Genomic DNA Released by Dying Cells Induces the Maturation of APCs | The Journal of Immunology
... dA)-poly(dT) (thick line) and poly(dG)-poly(dC) (thin line) or a mixture of ssPN composed of poly(dA) plus poly(dT) (dotted ... B, Raw 264.7 cells were transfected with 1 μg/ml dsPN or ssPN of defined length. All data shown in this figure are ... A, C57BL/6 mice (10/group) were immunized IP with 2 μg of OVA, or OVA plus 100 μg of ssPN (poly(dT)), dsPN (poly(dA)-poly(dT ... A, RAW 264.7 cells were transfected with increasing concentrations of dsPN (poly(dA)-poly(dT)) or ssPN (poly(dT)). Data ...
Synthesis of hybrid bacterial plasmids containing highly repeated satellite DNA. - PubMed - NCBI
... colony contained populations of circular molecules which varied both in the length of the satellite region and in the poly(dA ... dt) regions linking satellite and vector. While subcloning reduced the heterogeneity of these plasmid populations, continued ... Hybrid plasmid molecules containing tandemly repeated Drosophila satellite DNA were constructed using a modification of the (dA ... dT) homopolymer procedure of Lobban and Kaiser (1973). Recombinant plasmids recovered after transformation of recA bacteria ...
Digital transcriptome subtraction - Wikipedia
... such as poly-dT or poly-dA. Human repetitive DNA. Length - parameter is dependent on the optimized read length specific to the ... Messenger RNA is then purified using an oligo-dT column that binds to the poly-A tail, a signal specifically found on ... Minimum hit length for a positive match of human sequence is typically 30 consecutive identical bases, which equates to a BLAST ...
US4942227A - Bifunctional molecules having a DNA intercalator or DNA groove binder linked to ethylene diamine tetraacetic acid,...
... regions of pBR 322 with high poly (dA).poly(dT) content. Cleavage specificity diminishes at higher concentrations of BED.Fe(II ... Changes in the lengths of the resulting DNA fragments could be correlated with cleavage sites. Mapping restriction enzymes used ... Changes in the lengths of the resulting DNA fragments could be correlated with cleavage sites.) ... These fragments (167, 279, and 381 base pairs in length) were prepared by the usual methods from bacterial plasmid pBR322 and ...
RIG-I and MDA-5 Ligands | RLR Agonist | InvivoGen
Most popular agonists are triphosphate RNA (pppRNA) and ready to use complexed poly(I:C). ... Poly(dA:dT) RIG-I Agonist - CDS Agonist - AIM2 Inflammasome Inducer Poly(dA:dT) / LyoVec™ RIG-I Agonist - CDS Agonist - AIM2 ... Consistent with this, RIG-I and MDA-5 bind Poly(I:C), a synthetic dsRNA analog, with different length predilection . ... Poly(dA:dT), a synthetic analog of B-form DNA, thus constitutes another RIG-I ligand . ...
Helicos single molecule fluorescent sequencing - Wikipedia
... dT)50. To be compatible with the oligo(dT)50 primer on the flow cell surface, it is necessary to generate a poly(dA) tail of at ... dA) tails 90 to 200 nucleotides long. To generate tails of this length, it is first necessary to estimate how many 3 ends ... dT) on the surface. Generation of a 3 poly(dA) tail can be accomplished with a variety of different ligases or polymerases. If ... samples for sequencing are prepared in such a way that the poly(A) tail is longer than the oligo(dT)50 on the surface of the ...
Douglas L. Brutlag's Profile | Stanford Profiles
... colony contained populations of circular molecules which varied both in the length of the satellite region and in the poly(dA ... dt) regions linking satellite and vector. While subcloning reduced the heterogeneity of these plasmid populations, continued ... We also show that the length of an exon is a multiple of 3 more frequently than expected. Furthermore, the total length of two ... The repeat unit lengths were restricted to 5, 7, or 10 base pairs, with sequences closely related. Each sequence conforms to ...
Sequence specific detection of bacterial 23S ribosomal RNA by TLR13 | eLife
LPS and poly[dA:dT] treatment induced pro-IL-1β and mature IL-1β even in the absence of TLR13 (lanes 9 and 10). These results ... F) Full-length 23S rRNA or the RNA oligo corresponding to 2054-2068 of 23S was added to Raw264.7 cell lines stably expressing ... for 8 hr followed by transfection in the presence or absence of poly[dA:dT] (1.5 μg/ml) for 5 hr. Cell lysates (upper) and ... When the cells were stimulated with the 23S rRNA sequence and then transfected with the DNA poly[dA:dT], which activates the ...
Promoter regions of Plasmodium vivax are poorly or not recognized by Plasmodium falciparum | Malaria Journal | Full Text
... dA)poly(dT) tracts, poly(dAT) tracts and also GC rich motifs (data not shown). Using a matrix length of 27, the motif TGG [G/T] ... When the matrix length was set to six, a motif (GCATAT) identical among all Plasmodium species excepting P. vivax (GCANAN) was ... The matrix length was set from 4 to 20, which allowed the detection of very short and also longer and more complex conserved ... Pv ef-1α IR - P. vivax ef-1α intergenic region; LUC - luciferase; DT 3 - P. berghei dihydrofolate reductase 3 UTR. The EF ...
Poly [d(A-T)] powder
Mean strand length (gel electrophoresis): 500-100000 bp. Ratio dA/dT: 1:1±10%. A250/A260: 0.70-0.76. A280/A260: 0.38-0.58. A290 ... Use Poly[d(A-T)] as a template for RNA polymerases.. +- Specification Appearance: White lyophilizate. Melting range: 62.0-69.0° ... Poly[d(A-T)] is a suitable template for RNA synthesis.. +- Application ... Poly [d(A-T)] material number and pack size: Material Number. Pack Size. ...
Characterization of the parB-Like yyaA Gene of Bacillus subtilis | Journal of Bacteriology
The binding, however, was not specific, as it could be competed away with calf thymus DNA or poly(dA-dT)-poly(dG-dC) (Fig. 4B ... The lengths and positions of the fragments (a and b) used in the gel mobility shift assay are indicated. Note that the Sau3A ... while poly(dA-dT)-poly(dG-dC) (1 μg) was added to lanes 5 and 10. The reactions were carried out for 4 min at room temperature ...
Analysis of the spatial and temporal arrangement of transcripts over intergenic regions in the human malarial parasite...
Critically, the genomes of both organisms show evidence of extensive overrepresentation of homopolymeric poly(dA).poly(dT) ... dA).(dT) tracts that are prone to expansion could account for the increased length of UTR in P. falciparum. This proposal would ... and length of UTR (Figure 4C). For all UTR lengths investigated, the best-fit was achieved when 70-80% of the UTR is ... A length of UTR (fixed increments of 200 bp for whole genome or actual size of UTR for cohort of 105 genes used here) was ...
US Patent for Nanopore-facilitated single molecule detection of nucleic acids Patent (Patent # 9,574,228 issued February 21,...
The signal tag may be a charged polymer chain, which can be an oligonucleotide such as poly(dC)n, poly(dA)n, and or poly(dT)n, ... In still other embodiments, the nucleic acid extensions can range in length from about 5 to about 40 nucleotides, about 15 to ... For example, the signal tag may be oligonucleotides such as poly(dC)n, poly(dA)n, and poly(dT)n, or charged polypeptides. ... tag is more preferred over poly(dA) or poly(dT) tags; furthermore, the poly(dC)30 is much more efficient in generating ...
Eukaryotic genomes may exhibit up to 10 generic classes of gene promoters | BMC Genomics | Full Text
... and the middle-upper area shows different lengths of short homopolymer tracts (poly(dA), poly(dT), poly(dC), poly(dG)) disposed ... A) long Poly(dA) or Poly(dT) tracts or tandem short Poly(dA) or Poly(dT) tracts, (B) non-ordered short Poly(dA) and Poly(dT) ... dA) or long poly(dT) homopolymer tracts or tandem short poly(dA) or short poly(dT) tracts (Figure 1J). These promoters are ... The occurrence of long poly(dA:dT) or tandem short poly(dA:dT) tracts on short distances (,30b) near TSS, could also indicate ...
Repetitive DNA sequence - Bioinformatics.Org Wiki
The most abundant SSR tracts are the homopolymer repeats poly(dA).poly(dT) and poly(dG).poly(dC). Long (, 9 bp) tracts of both ... Many of the SSR tract monomer lengths can play a role in sequence-specific DNA binding by proteins . In coding regions, ... This is particularly true for poly(dA).poly(dT) tracts in the AT-rich genomes . ... Naturally occurring poly(dA-dT) sequences are upstream promoter elements for constitutive transcription in yeast. Proc Natl ...
A single domain antibody fragment that recognizes the adaptor ASC defines the role of ASC domains in inflammasome assembly | JEM
Cells were incubated with 200 ng/ml LPS in THP-1 medium for 3 h, followed by transfection of 1 µg/ml poly (dA:dT) with 1.75 µl/ ... Full-length human ASC and ASCCARD interacted with VHHASC, whereas murine ASC and the PYD of human ASC did not (Fig. 1 C). We ... 7, C and F). Indeed, we observed filamentous EGFP in poly(dA:dT)-transfected THP-1 cells expressing VHHASC-EGFP, but not in ... H) THP-1 cell lines cultivated as described in B were treated with LPS for 3 h and transfected with poly (dA:dT) or ...
Comprehensive Search for Alzheimer Disease Susceptibility Loci in the APOE Region
Roses et al10 reported an association between a variable length poly-T polymorphism ("poly-T") at rs10524523 in the gene ... Hixson JE, Vernier DT. Restriction isotyping of human apolipoprotein E by gene amplification and cleavage with HhaI. J Lipid ... Wittwer CT, Ririe KM, Andrew RV, David DA, Gundry RA, Balis UJ. The Light-Cycler: a microvolume multisample fluorimeter with ... TOMM40 poly-T repeat lengths, age of onset and psychosis risk in Alzheimer disease. Neurobiol Aging. 2011;32(12):2328-2329. e1- ...
Genome-wide mapping of nucleosome positioning in Toxoplasma gondii implicates nucleosome-free regions as the major gene...
Poly(dA:dT) is enriched at the +1 nucleosome rather than nucleosome-free regions (NFRs) and serves as the key sequence ... Nucleosome occupancy in exons is influenced by several factors, including GC content, length of exons and strength of splice ... Nucleosome occupancy in exons is influenced by several factors, including GC content, length of exons and strength of splice ... Poly(dA:dT) is enriched at the +1 nucleosome rather than nucleosome-free regions (NFRs) and serves as the key sequence ...
First: Principles: RNA Polymerase ||| and the RIG-I pathway
... dA-DT) was actually being converted into 5′-ppp. But how? It turns out that an enzyme uses the poly(dA-dT) as a template to ... Similar tests were done to determine the exact length of the poly. As few as 30 base pairs were able to trigger the IFN. But, ... Nucleic acids from the poly(dA-dT) cells were able to induce IFN- β, even in the presence of DNase I, so it wasnt DNA that was ... For example, a DNA sequence of repeating AT can induce it (Its known as poly(dA-dT). But no one knew how. Until a paper came ...
Dual Roles for Pax-6: a Transcriptional Repressor of Lens Fiber Cell-Specific β-Crystallin Genes | Molecular and Cellular...
250 ng of poly(dA-dT), 2.5 μg of bovine serum albumin, and 20,000 Cerenkov counts of double-stranded DNA probe. Nonradioactive ... The full-length Pax-6 cDNA cloned into pKW10 was previously described (13). The Pax-6 (5a) expression vector was generated by ... DNase I footprints were performed in the presence of 500 ng of poly(dA-dT) as previously described (20). ThePstI/PvuII fragment ... A) The full-length Pax-6 protein interacts specifically with the multimerized PL2 element. (A) EMSA demonstrates that the ...
For most - but not all - ESTs, the reverse transcription is primed by an oligo-dT, which hybridizes with the poly-A tail of ... Benson DA, Karsch-Mizrachi I, Lipman DJ, Ostell J, Wheeler DL. GenBank: update. Nucleic Acids Res. 2004 Jan 1;32(Database issue ... Note that the maximum intron length allowed by blat is 750,000 bases, which may eliminate some ESTs with very long introns that ... Before the cap-selection techniques emerged, some projects used random rather than poly-A priming in an attempt to retrieve ...
For most - but not all - ESTs, the reverse transcription is primed by an oligo-dT, which hybridizes with the poly-A tail of ... Benson DA, Karsch-Mizrachi I, Lipman DJ, Ostell J, Wheeler DL. GenBank: update. Nucleic Acids Res. 2004 Jan 1;32(Database issue ... Library: SAMN00150035 RIKEN full-length enriched human cDNA library, hippocampus. Clone: H023044H21. Read direction: 3 ( ... Note that the maximum intron length allowed by blat is 750,000 bases, which may eliminate some ESTs with very long introns that ...
Construction and selection of bacterial clones hybridized with the light-chain immunoglobulin mRNA<...
... the poly (dA) sequences were built up using terminal deoxynucleotidyl transferase. The building up of the poly(dT) sequences at ... The average length of connecting polynucleotide sequences was 50 nucleotides. After annealing and transformation of the cells ... the poly (dA) sequences were built up using terminal deoxynucleotidyl transferase. The building up of the poly(dT) sequences at ... the poly (dA) sequences were built up using terminal deoxynucleotidyl transferase. The building up of the poly(dT) sequences at ...
Keele Research Repository - Keele University
These key landmarks are all relatively depleted in nucleosomes in P. falciparum, as would be expected for poly dA.dT acting as ... Here we report an analysis of the relative frequency, length and spatial arrangement of homopolymer tracts for the complete P. ... Moreover, a striking pattern of enrichment of overly long poly dA.dT tracts in the intergenic regions of Plasmodium spp. ... In addition, using nucleosome-positioning data available for P. falciparum, we explore the correlation of poly dA.dT tracts ...
Mackerle - Finite Element Analysis of Fastening and Joining | Fracture Mechanics | Fatigue (Material)
Strength of DT tubular joints with brace and chord. compression. J Struct Engng, ASCE 1998;124(7):775 83.. 529. Kang CT, et al ... Nichols DA, Luscher AF. Numerical modeling of a post & dome snapfit feature. Res Engng Des 2000;12(2):10311.. 439. Nippard F, ... Influence of chord length and thickness on the. ultimate strength of out-of-plane bending loaded double-tee tubular. joints. ... polyethylene reactor. ASME/JSME Joint Pressure Vessels Piping. Conf, vol. PVP 371. New York: ASME; 1998. p. 18.. 536. Klasen B ...
Synthetic siRNA Detection Method - Patent application
The whole poly dT sequence region does not need to be annealed to the poly dA, and some of the poly dA region and/or some of ... poly dA is added to the 3 DNA end of at least one strand of siRNA to be detected to produce poly dA-added siRNA. The length of ... The poly dT sequence region is a homopolymer of deoxythymidine, and is annealed to a poly dA sequence region of the poly dA- ... with the poly dT sequence region is annealed to the dA region of the poly dA-added siRNA in the step (a), and DNA is elongated ...
DNA ligase IV from a basidiomycete, Coprinus cinereus, and its expression during meiosis | Microbiology Society
16/poly(dA) and (dT)16/poly(rA) as substrates, and had double-strand ligation activity, like human DNA ligase IV. Northern ... In addition, the recombinant CcLIG4 protein not only showed ATP-dependent ligase activity, but also used (dT) ... although the length and the percentages of aspartic and glutamic acids were similar. ... Expression of active human DNA ligase I in Escherichia coli cells that harbor a full-length DNA ligase I cDNA construct. J Biol ...
Herpes simplex virus 1 infection dampens the immediate early antiviral innate immunity signaling from peroxisomes by tegument...
- Messenger RNA is then purified using an oligo-dT column that binds to the poly-A tail, a signal specifically found on transcribed genes. (wikipedia.org)
- For most - but not all - ESTs, the reverse transcription is primed by an oligo-dT, which hybridizes with the poly-A tail of mature mRNA. (ucsc.edu)
- DNA is simply sheared, tailed with poly A, and hybridized to a flow cell surface containing oligo-dT for sequencing-by-synthesis of billions of molecules in parallel. (peaceportal.mobi)
- The target sequence attached to the flow cell surface could, in theory, be any sequence which can be synthesized, but, in practice, the standard commercially-available flow cell is oligo-dT Other sequences have been used successfully when there is a specific tag on the nucleic acid molecules of interest. (peaceportal.mobi)
- Since most work being carried out at the present time is with the oligo-dT surfaces, the discussion here will be restricted to that type of flow cell for simplicity. (peaceportal.mobi)
- dT) tracts are correlated with nucleosome-depleted regions in yeasts (7,11) and in humans (12,13). (psu.edu)
- Thus, poly(dA:dT) tracts within gene promoters can block nucleosome formation and increase transcri. (psu.edu)
- Nucleosome occupancy in exons is influenced by several factors, including GC content, length of exons and strength of splice sites. (omictools.com)
- Poly(dA:dT) is enriched at the +1 nucleosome rather than nucleosome-free regions (NFRs) and serves as the key sequence associated with nucleosome formation in T. gondii. (omictools.com)
- BACKGROUND: Homopolymeric tracts, particularly poly dA.dT, are enriched within the intergenic sequences of eukaryotic genomes where they appear to act as intrinsic regulators of nucleosome positioning. (keele.ac.uk)
- In addition, using nucleosome-positioning data available for P. falciparum, we explore the correlation of poly dA.dT tracts with nucleosome-positioning data over key expression landmarks within intergenic regions. (keele.ac.uk)
- These key landmarks are all relatively depleted in nucleosomes in P. falciparum, as would be expected for poly dA.dT acting as nucleosome exclusion sequences. (keele.ac.uk)
- A nucleosome configuration is a set of non-overlapping nucleosome positions on a single DNA molecule of defined length. (biomedcentral.com)
- In a foreign species, nucleosome depletion at promoters is maintained over poly(dA:dT) tracts, whereas internucleosome spacing and all other aspects of nucleosome positioning tested are not. (datamed.org)
- Strikingly, in a foreign species, nucleosome-depleted regions occur fortuitously in coding regions, and they often act as promoters that are associated with a positioned nucleosome array linked to the length of the transcription unit. (datamed.org)
- For example, nucleosome formation tends to occur at 10-bp periodic repeat of AT/TA dinucleotides and also GC-rich sequences, whereas poly(dA:dT) sequences tend to evict nucleosomes and thus reside in the linker region [ 8 , 9 ]. (biomedcentral.com)
- dT) homopolymer tracts in D. discoideum gene flanking and intron sequences. (bioinformatics.org)
- Naturally occurring poly(dA-dT) sequences are upstream promoter elements for constitutive transcription in yeast. (bioinformatics.org)
- dT) homopolymer tracts in gene flanking sequences suggests nucleosomal periodicity in D. discoideum DNA. (bioinformatics.org)
- This copy was completed with another chain without adding the exogenous primer, with the aid of the Klenov fragment of DNA polymerase I. After treatment of the double-stranded pin DNA with endonuclease S1, the poly (dA) sequences were built up using terminal deoxynucleotidyl transferase. (elsevier.com)
- The building up of the poly(dT) sequences at the DNA 3'-termini of pBR322 plasmid previously treated with BamHI was performed in a similar way. (elsevier.com)
- The average length of connecting polynucleotide sequences was 50 nucleotides. (elsevier.com)
- ESTs are single-read sequences, typically about 500 bases in length, that usually represent fragments of transcribed genes. (ucsc.edu)
- The most abundant SSR tracts are the homopolymer repeats poly(dA).poly(dT) and poly(dG).poly(dC). (bioinformatics.org)
- This is particularly true for poly(dA).poly(dT) tracts in the AT-rich genomes . (bioinformatics.org)
- A previous study of the incomplete genome of the human malarial parasite Plasmodium falciparum reports a higher than expected enrichment of poly dA.dT tracts, far above that anticipated even in this highly AT rich genome. (keele.ac.uk)
- Here we report an analysis of the relative frequency, length and spatial arrangement of homopolymer tracts for the complete P. falciparum genome, extending this analysis to twelve additional genomes of Apicomplexan parasites important to human and animal health. (keele.ac.uk)
- Moreover, a striking pattern of enrichment of overly long poly dA.dT tracts in the intergenic regions of Plasmodium spp. (keele.ac.uk)
- Luciferase reporter plasmids harboring the upstream regions of the msp1 , dhfr , and vir3 genes as well as the full-length intergenic regions of the vir23/24 and ef-1α genes of P. vivax were constructed and transiently transfected in P. falciparum . (biomedcentral.com)
- Only the constructs with the full-length intergenic regions of the vir23/24 and ef-1α genes were recognized by the P. falciparum transcription machinery albeit to values approximately two orders of magnitude lower than those reported by luc plasmids harbouring promoter regions from P. falciparum and Plasmodium berghei . (biomedcentral.com)
- Poly(dA:dT)/LyoVec complexes that are indirectly recognized by RIG-I. (invivogen.com)
- THP1-Dual™ cells were stimulated with 1 ng/ml Pam3CSK4 (TLR2), 100 ng/ml LPS-EK UP (TLR4), 100 ng/ml FLA-ST UP (TLR5), 10 μg/ml R848 (TLR7/8), 10 μg/ml Tri-DAP (NOD1), 3 μg/ml 2'3'-cGAMP (STING), 1 μg/ml poly(I:C)/LyoVec™ (RLR) or 100 ng/ml poly(dA:dT)/LyoVec™ (CDS). (invivogen.com)
- However, it is admitted that RIG-I and MDA-5 exhibit a different dependency for the length of dsRNAs: RIG-I selectively binds short dsRNA while MDA-5 selectively binds long dsRNA. (invivogen.com)
- The role of dT n extends beyond termination, since it provides a means to link Pol III transcripts to La, an abundant and ubiquitous nuclear phosphoprotein that binds these RNAs in a 3′-oligo(U) length-dependent manner and promotes their posttranscriptional processing ( 27 , 32 , 37 ). (pubmedcentralcanada.ca)
- Low complexity - complexity score of a sequence reflects number of identical bases in a series (homo-polymers) such as poly-dT or poly-dA. (wikipedia.org)
- Many of the SSR tract monomer lengths can play a role in sequence-specific DNA binding by proteins . (bioinformatics.org)
- For example, a DNA sequence of repeating AT can induce it (It's known as poly(dA-dT). (benfulton.net)
- Before the cap-selection techniques emerged, some projects used random rather than poly-A priming in an attempt to retrieve sequence distant from the 3' end. (ucsc.edu)
- The inserted sequence had no sequence identity with other eukaryotic counterparts of DNA ligase IV or with another aspartic acid and glutamic acid rich sequence inserted in C. cinereus proliferating cell nuclear antigen (CcPCNA), although the length and the percentages of aspartic and glutamic acids were similar. (microbiologyresearch.org)
- To be considered spliced, an EST must show evidence of at least one canonical intron (i.e., the genomic sequence between EST alignment blocks must be at least 32 bases in length and have GT/AG ends). (ucsc.edu)
- 4. The method of claim 2 wherein said first oligonucleotide comprises a primer containing an oligo or poly dT sequence. (freepatentsonline.com)
- 5. The method of claim 4 wherein said oligo or poly dT sequence is at least, or about, eight dT in length. (freepatentsonline.com)
- The Helicos Genetic Analysis System http: If starting with nucleic acids longer than nt, it is generally heliacope to shear the nucleic acids to an average length of nt so that more sequence information can be generated from the same mass of nucleic acids. (peaceportal.mobi)
- Length polymorphisms of simple sequence repeat DNA in soybean. (edu.pl)
- Here, we show that poly(dA:dT) is a novel promoter element whose function depends on its intrinsic structure, not its interaction with sequence-specific, DNA-binding proteins. (saladgaffe.tk)
- Pax-6 constructs lacking the C-terminal activation domain repressed βB1-crystallin promoter activity as effectively as the full-length protein, but the PD alone or Pax-6 (5a), a splice variant with an altered PD affecting its DNA binding specificity, did not. (asm.org)
- There is a conserved spatial arrangement of poly dA.dT immediately flanking open reading frames and over predicted core promoter sites. (keele.ac.uk)
- MicroRNAs (miRNAs) are small non-coding RNAs of about 21 to 24 nucleotides in length and are derived from introns and exons of both protein-coding and non-coding genes. (biomedcentral.com)
- The 3′ ends of tRNA and other RNA polymerase III (Pol III)-dependent genes contain dT n termination signals at which Pol III pauses and releases its RNA ( 6 ). (pubmedcentralcanada.ca)
- Recombinant plasmids isolated from a single colony contained populations of circular molecules which varied both in the length of the satellite region and in the poly(dA)-(dt) regions linking satellite and vector. (nih.gov)
- In addition, the recombinant CcLIG4 protein not only showed ATP-dependent ligase activity, but also used (dT) 16 /poly(dA) and (dT) 16 /poly(rA) as substrates, and had double-strand ligation activity, like human DNA ligase IV. (microbiologyresearch.org)
- Moreover, transfection of the pUL83 expression vector into recombinant HEK293T cells stimulated by poly(dA:dT) resulted in reduced expression and activation of AIM2 inflammasome-associated proteins, compared with the absence of pUL83. (biomedcentral.com)
- Furthermore, the Terminal Deoxynucleotidyl Transferase will not use RNA as a template in the T-tailing step, and the use of a poly(dA) primer, instead of a poly(dT) primer, makes priming to RNA unlikely. (clontech.com)
- Together, the DNA SMART Oligo, DNA SMART Poly(dA) Primer, and the Indexing Primers for Illumina ® add 153 bp to the initial DNA fragment length. (clontech.com)
- Helicos recommends a length less than 1000nt (nucleotides), optimally about 100-200nt. (wikipedia.org)
- If there is sufficient DNA to measure both mass and average length, it is possible to determine the proper amount of dATP to be added to generate poly(dA) tails 90 to 200 nucleotides long. (wikipedia.org)
- MicroRNAs (miRNAs) are small non-coding RNAs (about 21 to 24 nucleotides in length) that effectively reduce the translation of their target mRNAs. (biomedcentral.com)
- The processing of extra- and intracellular MWNTs (both pristine and when acid oxidised) by microglia was characterised across multiple length scales by correlating a range of dynamic, quantitative and multi-scale techniques, including: UV-vis spectroscopy, light microscopy, focussed ion beam scanning electron microscopy and transmission electron microscopy. (imperial.ac.uk)
- Two monoculture models, immortalized human alveolar epithelial type 1 (TT1) cells and primary human alveolar epithelial type 2 cells (AT2), which constitute the alveolar epithelial barrier, were employed to investigate the uptake and transport of 300 and 700 nm in length, poly(4-vinylpyridine)-functionalized, multi-walled carbon nanotubes (p(4VP)-MWCNTs) using quantitative imaging and spectroscopy techniques. (imperial.ac.uk)
- A model system that can be used to alter 3′-U length and study its consequences should be helpful in understanding functional connectivity between Pol III termination and posttranscriptional processing. (pubmedcentralcanada.ca)
- An opal suppressor tRNA Ser UGAM gene with a dT 7 terminator ( tRNAmSer7T ), which suppresses a nonsense mutation in the ade6 - 704 allele and accumulation of red pigment, has been used to study tRNA biogenesis and the function of the La homolog, Sla1p, in the yeast S. pombe . (pubmedcentralcanada.ca)