Proteins that share the common characteristic of binding to carbohydrates. Some ANTIBODIES and carbohydrate-metabolizing proteins (ENZYMES) also bind to carbohydrates, however they are not considered lectins. PLANT LECTINS are carbohydrate-binding proteins that have been primarily identified by their hemagglutinating activity (HEMAGGLUTININS). However, a variety of lectins occur in animal species where they serve diverse array of functions through specific carbohydrate recognition.
Protein or glycoprotein substances of plant origin that bind to sugar moieties in cell walls or membranes. Some carbohydrate-metabolizing proteins (ENZYMES) from PLANTS also bind to carbohydrates, however they are not considered lectins. Many plant lectins change the physiology of the membrane of BLOOD CELLS to cause agglutination, mitosis, or other biochemical changes. They may play a role in plant defense mechanisms.
A subclass of lectins that are specific for CARBOHYDRATES that contain MANNOSE.
Lectins purified from the germinating seeds of common wheat (Triticum vulgare); these bind to certain carbohydrate moieties on cell surface glycoproteins and are used to identify certain cell populations and inhibit or promote some immunological or physiological activities. There are at least two isoforms of this lectin.
A class of animal lectins that bind to carbohydrate in a calcium-dependent manner. They share a common carbohydrate-binding domain that is structurally distinct from other classes of lectins.
A class of animal lectins that bind specifically to beta-galactoside in a calcium-independent manner. Members of this class are distiguished from other lectins by the presence of a conserved carbohydrate recognition domain. The majority of proteins in this class bind to sugar molecules in a sulfhydryl-dependent manner and are often referred to as S-type lectins, however this property is not required for membership in this class.
Carbohydrates covalently linked to a nonsugar moiety (lipids or proteins). The major glycoconjugates are glycoproteins, glycopeptides, peptidoglycans, glycolipids, and lipopolysaccharides. (From Biochemical Nomenclature and Related Documents, 2d ed; From Principles of Biochemistry, 2d ed)
Lectin purified from peanuts (ARACHIS HYPOGAEA). It binds to poorly differentiated cells and terminally differentiated cells and is used in cell separation techniques.
The largest class of organic compounds, including STARCH; GLYCOGEN; CELLULOSE; POLYSACCHARIDES; and simple MONOSACCHARIDES. Carbohydrates are composed of carbon, hydrogen, and oxygen in a ratio of Cn(H2O)n.
A MANNOSE/GLUCOSE binding lectin isolated from the jack bean (Canavalia ensiformis). It is a potent mitogen used to stimulate cell proliferation in lymphocytes, primarily T-lymphocyte, cultures.
Glycosides formed by the reaction of the hydroxyl group on the anomeric carbon atom of galactose with an alcohol to form an acetal. They include both alpha- and beta-galactosides.
A plant genus in the family LILIACEAE (sometimes classified as Amaryllidaceae). Galanthus nivalis L. is the source of GALANTHAMINE.
The N-acetyl derivative of galactosamine.
A family of calcium-binding alpha-globulins that are synthesized in the LIVER and play an essential role in maintaining the solubility of CALCIUM in the BLOOD. In addition the fetuins contain aminoterminal cystatin domains and are classified as type 3 cystatins.
Glycoprotein molecules on the surface of B- and T-lymphocytes, that react with molecules of antilymphocyte sera, lectins, and other agents which induce blast transformation of lymphocytes.
The aggregation of ERYTHROCYTES by AGGLUTININS, including antibodies, lectins, and viral proteins (HEMAGGLUTINATION, VIRAL).
Cellular processes in biosynthesis (anabolism) and degradation (catabolism) of CARBOHYDRATES.
The clumping together of suspended material resulting from the action of AGGLUTININS.
A hexose or fermentable monosaccharide and isomer of glucose from manna, the ash Fraxinus ornus and related plants. (From Grant & Hackh's Chemical Dictionary, 5th ed & Random House Unabridged Dictionary, 2d ed)
The sequence of carbohydrates within POLYSACCHARIDES; GLYCOPROTEINS; and GLYCOLIPIDS.
A plant genus of the family FABACEAE. Canavalia ensiformis is the source of CONCANAVALIN A.
A plant genus of the family MORACEAE. Puag-haad extract, from A. lakoocha, contains STILBENES and related 4-substituted RESORCINOLS.
A plant genus of the family EUPHORBIACEAE, order Euphorbiales, subclass Rosidae. The seed of Ricinus communis L. is the CASTOR BEAN which is the source of CASTOR OIL; RICIN; and other lectins.
An aldohexose that occurs naturally in the D-form in lactose, cerebrosides, gangliosides, and mucoproteins. Deficiency of galactosyl-1-phosphate uridyltransferase (GALACTOSE-1-PHOSPHATE URIDYL-TRANSFERASE DEFICIENCY DISEASE) causes an error in galactose metabolism called GALACTOSEMIA, resulting in elevations of galactose in the blood.
Ribosome inactivating proteins consisting of two polypeptide chains, the toxic A subunit and a lectin B subunit, linked by disulfide bridges. The lectin portion binds to cell surfaces and facilitates transport into the ENDOPLASMIC RETICULUM.
Agents that cause agglutination of red blood cells. They include antibodies, blood group antigens, lectins, autoimmune factors, bacterial, viral, or parasitic blood agglutinins, etc.
Substances, usually of biological origin, that cause cells or other organic particles to aggregate and stick to each other. They include those ANTIBODIES which cause aggregation or agglutination of particulate or insoluble ANTIGENS.
Sensitive tests to measure certain antigens, antibodies, or viruses, using their ability to agglutinate certain erythrocytes. (From Stedman, 26th ed)
Endogenous glycoproteins from which SIALIC ACID has been removed by the action of sialidases. They bind tightly to the ASIALOGLYCOPROTEIN RECEPTOR which is located on hepatocyte plasma membranes. After internalization by adsorptive ENDOCYTOSIS they are delivered to LYSOSOMES for degradation. Therefore receptor-mediated clearance of asialoglycoproteins is an important aspect of the turnover of plasma glycoproteins. They are elevated in serum of patients with HEPATIC CIRRHOSIS or HEPATITIS.
Glycosides formed by the reaction of the hydroxyl group on the anomeric carbon atom of mannose with an alcohol to form an acetal. They include both alpha- and beta-mannosides.
Conjugated protein-carbohydrate compounds including mucins, mucoid, and amyloid glycoproteins.
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
The N-acetyl derivative of glucosamine.
Parasitic plants that form a bushy growth on branches of host trees which are in the order Santalales. It includes the Christmas mistletoe family (VISCACEAE), the showy mistletoe family (LORANTHACEAE) and the catkin mistletoe family (Eremolepidaceae). The composition of toxins, lectins, tyramine, phenethylamines, and other compounds may be affected by the host.
A chromatographic technique that utilizes the ability of biological molecules to bind to certain ligands specifically and reversibly. It is used in protein biochemistry. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)
N-Glycosidases that remove adenines from RIBOSOMAL RNA, depurinating the conserved alpha-sarcin loop of 28S RIBOSOMAL RNA. They often consist of a toxic A subunit and a binding lectin B subunit. They may be considered as PROTEIN SYNTHESIS INHIBITORS. They are found in many PLANTS and have cytotoxic and antiviral activity.
Plants whose roots, leaves, seeds, bark, or other constituent parts possess therapeutic, tonic, purgative, curative or other pharmacologic attributes, when administered to man or animals.
Carbohydrates consisting of between two (DISACCHARIDES) and ten MONOSACCHARIDES connected by either an alpha- or beta-glycosidic link. They are found throughout nature in both the free and bound form.
The large family of plants characterized by pods. Some are edible and some cause LATHYRISM or FAVISM and other forms of poisoning. Other species yield useful materials like gums from ACACIA and various LECTINS like PHYTOHEMAGGLUTININS from PHASEOLUS. Many of them harbor NITROGEN FIXATION bacteria on their roots. Many but not all species of "beans" belong to this family.
An N-acyl derivative of neuraminic acid. N-acetylneuraminic acid occurs in many polysaccharides, glycoproteins, and glycolipids in animals and bacteria. (From Dorland, 28th ed, p1518)
Cell surface receptors that bind to ACETYLGLUCOSAMINE.
The chemical or biochemical addition of carbohydrate or glycosyl groups to other chemicals, especially peptides or proteins. Glycosyl transferases are used in this biochemical reaction.
Mannosides formed by the reaction of the hydroxyl group on the anomeric carbon atom of mannose with methyl alcohol. They include both alpha- and beta-methylmannosides.
A toxic lectin from the seeds of jequirity, Abrus precatorius L. Very active poison. Five different proteins have so far been isolated: Abrus agglutinin, the component responsible for: hemagglutinating activity, & abrins a-d, the toxic principals each consisting of two peptide chains are held together by disulfide bonds.
Mucoproteins isolated from the kidney bean (Phaseolus vulgaris); some of them are mitogenic to lymphocytes, others agglutinate all or certain types of erythrocytes or lymphocytes. They are used mainly in the study of immune mechanisms and in cell culture.
The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.
Simple sugars, carbohydrates which cannot be decomposed by hydrolysis. They are colorless crystalline substances with a sweet taste and have the same general formula CnH2nOn. (From Dorland, 28th ed)
A genus of leguminous shrubs or trees, mainly tropical, yielding useful compounds such as ALKALOIDS and PLANT LECTINS.
A galectin found abundantly in smooth muscle (MUSCLE, SMOOTH) and SKELETAL MUSCLE and many other tissues. It occurs as a homodimer with two 14-kDa subunits.
Study of intracellular distribution of chemicals, reaction sites, enzymes, etc., by means of staining reactions, radioactive isotope uptake, selective metal distribution in electron microscopy, or other methods.
A plant genus in the family CAPRIFOLIACEAE known for elderberries.
The process in which substances, either endogenous or exogenous, bind to proteins, peptides, enzymes, protein precursors, or allied compounds. Specific protein-binding measures are often used as assays in diagnostic assessments.
A group of naturally occurring N-and O-acyl derivatives of the deoxyamino sugar neuraminic acid. They are ubiquitously distributed in many tissues.
The parts of a macromolecule that directly participate in its specific combination with another molecule.
Glycoprotein moieties on the surfaces of cell membranes that bind concanavalin A selectively; the number and location of the sites depends on the type and condition of the cell.
A multifunctional galactin initially discovered as a macrophage antigen that binds to IMMUNOGLOBULIN E, and as 29-35-kDa lectin that binds LAMININ. It is involved in a variety of biological events including interactions with galactose-containing glycoconjugates, cell proliferation, CELL DIFFERENTIATION, and APOPTOSIS.
Electrophoresis in which a polyacrylamide gel is used as the diffusion medium.
The characteristic 3-dimensional shape of a carbohydrate.
Tests that are dependent on the clumping of cells, microorganisms, or particles when mixed with specific antiserum. (From Stedman, 26th ed)
Any compound that contains a constituent sugar, in which the hydroxyl group attached to the first carbon is substituted by an alcoholic, phenolic, or other group. They are named specifically for the sugar contained, such as glucoside (glucose), pentoside (pentose), fructoside (fructose), etc. Upon hydrolysis, a sugar and nonsugar component (aglycone) are formed. (From Dorland, 28th ed; From Miall's Dictionary of Chemistry, 5th ed)
A class of C-type lectins that target the carbohydrate structures found on invading pathogens. Binding of collectins to microorganisms results in their agglutination and enhanced clearance. Collectins form trimers that may assemble into larger oligomers. Each collectin polypeptide chain consists of four regions: a relatively short N-terminal region, a collagen-like region, an alpha-helical coiled-coil region, and carbohydrate-binding region.
A galectin found in the small and large intestine and the stomach. It occurs as a homodimer with two 36-kDa subunits and is localized to sites of cell adhesion where it may play role in assembly of ADHERENS JUNCTIONS.
Common name for Ricinus communis, a species in the family EUPHORBIACEAE. It is the source of CASTOR OIL.
A plant genus in the family ARALIACEAE, order Apiales, subclass Rosidae. It includes Aralia californica S. Watson, Aralia nudicaulis L., and Aralia racemosa L.
A class of Echinodermata characterized by long, slender bodies.
An enzyme that catalyzes the hydrolysis of alpha-2,3, alpha-2,6-, and alpha-2,8-glycosidic linkages (at a decreasing rate, respectively) of terminal sialic residues in oligosaccharides, glycoproteins, glycolipids, colominic acid, and synthetic substrate. (From Enzyme Nomenclature, 1992)
A disaccharide of GLUCOSE and GALACTOSE in human and cow milk. It is used in pharmacy for tablets, in medicine as a nutrient, and in industry.
A plant species of the genus DATURA, family SOLANACEAE, that contains TROPANES and other SOLANACEOUS ALKALOIDS.
A protein phytotoxin from the seeds of Ricinus communis, the castor oil plant. It agglutinates cells, is proteolytic, and causes lethal inflammation and hemorrhage if taken internally.
A monocot family within the order Liliales. This family is divided by some botanists into other families such as Convallariaceae, Hyacinthaceae and Amaryllidaceae. Amaryllidaceae, which have inferior ovaries, includes CRINUM; GALANTHUS; LYCORIS; and NARCISSUS and are known for AMARYLLIDACEAE ALKALOIDS.
A plant genus of the family Musaceae, order Zingiberales, subclass Zingiberidae, class Liliopsida.
The encapsulated embryos of flowering plants. They are used as is or for animal feed because of the high content of concentrated nutrients like starches, proteins, and fats. Rapeseed, cottonseed, and sunflower seed are also produced for the oils (fats) they yield.
Proteins which are present in or isolated from SOYBEANS.
Proteins which contain carbohydrate groups attached covalently to the polypeptide chain. The protein moiety is the predominant group with the carbohydrate making up only a small percentage of the total weight.
A plant species of the family FABACEAE that yields edible seeds, the familiar peanuts, which contain protein, oil and lectins.
The major human blood type system which depends on the presence or absence of two antigens A and B. Type O occurs when neither A nor B is present and AB when both are present. A and B are genetic factors that determine the presence of enzymes for the synthesis of certain glycoproteins mainly in the red cell membrane.
The sum of the weight of all the atoms in a molecule.
A plant genus of the family FABACEAE. Members contain isoacteoside, luteolin, indole-3-carboxylic acid.
An arthropod subclass (Xiphosura) comprising the North American (Limulus) and Asiatic (Tachypleus) genera of horseshoe crabs.
Multicellular, eukaryotic life forms of kingdom Plantae (sensu lato), comprising the VIRIDIPLANTAE; RHODOPHYTA; and GLAUCOPHYTA; all of which acquired chloroplasts by direct endosymbiosis of CYANOBACTERIA. They are characterized by a mainly photosynthetic mode of nutrition; essentially unlimited growth at localized regions of cell divisions (MERISTEMS); cellulose within cells providing rigidity; the absence of organs of locomotion; absence of nervous and sensory systems; and an alternation of haploid and diploid generations.
Specific, characterizable, poisonous chemicals, often PROTEINS, with specific biological properties, including immunogenicity, produced by microbes, higher plants (PLANTS, TOXIC), or ANIMALS.
The first alpha-globulins to appear in mammalian sera during FETAL DEVELOPMENT and the dominant serum proteins in early embryonic life.
A specific mannose-binding member of the collectin family of lectins. It binds to carbohydrate groups on invading pathogens and plays a key role in the MANNOSE-BINDING LECTIN COMPLEMENT PATHWAY.
The systematic study of the structure and function of the complete set of glycans (the glycome) produced in a single organism and identification of all the genes that encode glycoproteins.
The degree of similarity between sequences of amino acids. This information is useful for the analyzing genetic relatedness of proteins and species.
SUGARS containing an amino group. GLYCOSYLATION of other compounds with these amino sugars results in AMINOGLYCOSIDES.

Lymphocyte proliferation inhibitory factor (PIF) in alcoholic liver disease. (1/6088)

Lymphocyte proliferation inhibitory factor (PIF) was determined in the supernatants of PHA-stimulated lymphocytes from patients with alcoholic liver disease. PIF was assayed by determining inhibition of DNA synthesis in WI-38 human lung fibroblasts. A two-fold greater inhibition in thymidine incorporation into DNA by lung fibroblasts was observed in supernatants of PHA stimulated lymphocytes from patients with alcoholic hepatitis or active Laennec's cirrhosis as compared with that found in control subjects or patients with fatty liver. It is suggested that decreased liver cell regeneration seen in some patients with alcoholic hepatitis may be due to increased elaboration of PIF.  (+info)

Structure of CD94 reveals a novel C-type lectin fold: implications for the NK cell-associated CD94/NKG2 receptors. (2/6088)

The crystal structure of the extracellular domain of CD94, a component of the CD94/NKG2 NK cell receptor, has been determined to 2.6 A resolution, revealing a unique variation of the C-type lectin fold. In this variation, the second alpha helix, corresponding to residues 102-112, is replaced by a loop, the putative carbohydrate-binding site is significantly altered, and the Ca2+-binding site appears nonfunctional. This structure may serve as a prototype for other NK cell receptors such as Ly-49, NKR-P1, and CD69. The CD94 dimer observed in the crystal has an extensive hydrophobic interface that stabilizes the loop conformation of residues 102-112. The formation of this dimer reveals a putative ligand-binding region for HLA-E and suggests how NKG2 interacts with CD94.  (+info)

Differential expression of the mRNA for the vanilloid receptor subtype 1 in cells of the adult rat dorsal root and nodose ganglia and its downregulation by axotomy. (3/6088)

Sensitivity to the pungent vanilloid, capsaicin, defines a subpopulation of primary sensory neurons that are mainly polymodal nociceptors. The recently cloned vanilloid receptor subtype 1 (VR1) is activated by capsaicin and noxious heat. Using combined in situ hybridization and histochemical methods, we have characterized in sensory ganglia the expression of VR1 mRNA. We show that this receptor is almost exclusively expressed by neurofilament-negative small- and medium-sized dorsal root ganglion cells. Within this population, VR1 mRNA is detected at widely varying levels in both the NGF receptor (trkA)-positive, peptide-producing cells that elicit neurogenic inflammation and the functionally less characterized glial cell line-derived neurotrophic factor-responsive cells that bind lectin Griffonia simplicifolia isolectin B4 (IB4). Cells without detectable levels of VR1 mRNA are found in both classes. A subpopulation of the IB4-binding cells that produce somatostatin has relatively low levels of VR1 mRNA. A previously uncharacterized population of very small cells that express the receptor tyrosine kinase (RET) and that do not label for trkA or IB4-binding has the highest relative levels of VR1 mRNA. The majority of small visceral sensory neurons of the nodose ganglion also express VR1 mRNA, in conjunction with the BDNF receptor trkB but not trkA. Axotomy results in the downregulation of VR1 mRNA in dorsal root ganglion cells. Our data emphasize the heterogeneity of VR1 mRNA expression by subclasses of small sensory neurons, and this may result in their differential sensitivity to chemical and noxious heat stimuli. Our results also indicate that peripherally derived trophic factors may regulate levels of VR1 mRNA.  (+info)

Control of metastasis by Asn-linked, beta1-6 branched oligosaccharides in mouse mammary cancer cells. (4/6088)

Studies in cell lines and malignant human tissues have shown that increased cell-surface Asn-linked beta1-6(GlcNAcbeta1-6Man) branching is associated with increased tumorigenic and metastatic properties. In this study, three mouse mammary cancer cell lines were transfected with an expression vector containing the mouse cDNA for N-acetylglucosaminyltransferase V (GlcNAcT-V EC, the glycosyltransferase responsible for initiating beta1-6 branching on Asn-linked carbohydrates. The cell lines were screened for increased cytotoxicity to L-PHA, a lectin specific for beta1-6 branching structures. Cell lines exhibiting increased L-PHA cytotoxicity expressed increased levels of beta1-6 branching structures. Northern blots detected the presence of GlcNAcT-V transcribed from the expression vector in the L-PHA sensitive cell lines. After injection into the tail veins of mice, transfected cell lines with increased beta1-6 branching on the cell surface formed elevated levels of lung tumors relative to control transfected cell lines (P < 0.002). Western blots of membrane proteins from GlcNAcT-V transfected and control cells probed with the lectins DSA and WGA did not show an increase in polyN-acetyllactosamine and sialic acid content in the transfected cell lines. These results demonstrate that a specific increase in beta1-6 branching due to an elevation in GlcNAcT-V expression increases metastatic potential.  (+info)

Lectin receptor sites on rat liver cell nuclear membranes. (5/6088)

The presence and localization of lectin receptor sites on rat liver cell nuclear and other endomembranes was studied by light and electron microscopy using fluorescein and ferritin-coupled lectin conjugates. Isolated nuclei labelled with fluorescein-conjugated Concanavalin A (Con A) or wheat germ agglutinin (WGA) often showed membrane staining, which sometimes was especially bright on small stretches of the nuclear surface. Unlabelled nuclei and nuclei with a complete ring fluorescence were also seen. The nuclear fluorescence corresponded in intensity to that seen on the surface of isolated rat liver cells. Con A-ferritin particles were seldom detected on the cytoplasmic surface of the intact nuclear envelope. However, at places where the 2 leaflets of the envelope were widely separated or where the outer nuclear membrane was partly torn away, heavy labelling was seen on the cisternal surface of both the inner and outer nuclear membranes. Labelling with Con A-ferritin was also found on the cisternal side of rough endoplasmic reticulum present in the specimens. No labelling was seen on the cytoplasmic surface of mitochondrial outer membrane. The results demonstrate the presence of binding sites for Con A and WGA in nuclei and an asymmetric localization of these sites on the cisternal side of ribosome-carrying endomembranes in rat liver cells.  (+info)

Developmental changes in mucosubstances revealed by immunostaining with antimucus monoclonal antibodies and lectin staining in the epithelium lining the segment from gizzard to duodenum of the chick embryo. (6/6088)

The mucosubstances in the epithelium lining the segment from gizzard to duodenum during development of the chick embryo was studied histochemically using monoclonal antibodies against gizzard mucus and lectins, with attention to the regional differentiation of the epithelium in this segment. The anterior limit of epithelial CdxA mRNA expression detected by in situ hybridisation, which served as the position of the gizzard-duodenal boundary, was clearly found from d 3. Granules positive for some antibodies or lectins were found in the region ranging from the posterior part of the gizzard to the duodenum at d 3, which was followed by an increase in the number of granules and a gradual enlargement of the granule-positive area to the anterior part of the gizzard over 4-6 d. From d 4, the epithelia of the gizzard body and of the pyloric or duodenal region came to be differently stained with some antibodies or lectins. From d 10, each region showed a specific pattern of staining. The epithelia of the gizzard body and pyloric region contained abundant mucus granules with a different staining pattern. In the duodenum the number of stained granules was low except in occasional goblet cells. Thus the epithelia of the gizzard body, pyloric region and duodenum may produce different mucosubstances and the regional differentiation in these epithelia may start at rather early stages soon after the formation of digestive tube.  (+info)

Effect of sodium butyrate on lymphocyte activation. (7/6088)

Butyrate, in relatively low concentrations, has been shown to induce synthesis of enzymes, cause changes in cell morphology, and inhibit growth of a variety of mammalian cells in tissue culture (reviewed in [1]). In this communication, we report our observations on the effect of butyrate on lymphocyte activation. Butyrate completely and reversibly inhibits mitogen-induced blast formation. We present evidence that it does not interfere with the binding of mitogens, that it does not inhibit a number of the "early" reactions involved in activation, and that it does not affect ongoing DNA synthesis for an extended period of time. However, butyrate rapidly inhibits any increase in the rate of DNA synthesis.  (+info)

In vivo NGF deprivation reduces SNS expression and TTX-R sodium currents in IB4-negative DRG neurons. (8/6088)

Recent evidence suggests that changes in sodium channel expression and localization may be involved in some pathological pain syndromes. SNS, a tetrodotoxin-resistant (TTX-R) sodium channel, is preferentially expressed in small dorsal root ganglion (DRG) neurons, many of which are nociceptive. TTX-R sodium currents and SNS mRNA expression have been shown to be modulated by nerve growth factor (NGF) in vitro and in vivo. To determine whether SNS expression and TTX-R currents in DRG neurons are affected by reduced levels of systemic NGF, we immunized adult rats with NGF, which causes thermal hypoalgesia in rats with high antibody titers to NGF. DRG neurons cultured from rats with high antibody titers to NGF, which do not bind the isolectin IB4 (IB4(-)) but do express TrkA, were studied with whole cell patch-clamp and in situ hybridization. Mean TTX-R sodium current density was decreased from 504 +/- 77 pA/pF to 307 +/- 61 pA/pF in control versus NGF-deprived neurons, respectively. In comparison, the mean TTX-sensitive sodium current density was not significantly different between control and NGF-deprived neurons. Quantification of SNS mRNA hybridization signal showed a significant decrease in the signal in NGF-deprived neurons compared with the control neurons. The data suggest that NGF has a major role in the maintenance of steady-state levels of TTX-R sodium currents and SNS mRNA in IB4(-) DRG neurons in adult rats in vivo.  (+info)

TY - JOUR. T1 - Distribution of Bandeiraea simplicifolia lectin binding sites in the genital organs of female and male mice. AU - Wu, T. C.. AU - Wan, Yu-Jui Yvonne. AU - Damjanov, I.. PY - 1983/6. Y1 - 1983/6. N2 - Fluorescein isothiocyanate labelled type I lectin from Bandeiraea simplicifolia (BSA-I) known for its specific binding to α-d-galactopyranosyl and 2-acetamido-2-deoxy-d-galactose groups, has been used to map the distribution of the lectin specific binding sites in the genital organs of female and male mice. In non-pregnant female mice, strong lectin reactivity was restricted to the epithelium of the distal oviduct, the cervix and vagina. In pregnant mice strong BSA-I reactivity was also noted in the epithelium of uterine glands from the time of implantation on day 5 onward. In the testis BSA-I bound selectively to sperm but did not react with other cells in the seminiferous tubules. In the proximal caput epididymis BSA-I reacted with the epithelial cells, the underlying basement ...
Because of the antimicrobial resistance crisis, lectins are considered novel drug targets. Pseudomonas aeruginosa utilizes LecA and LecB in the infection process. Inhibition of both lectins with carbohydrate‐derived molecules can reduce biofilm formation to restore antimicrobial susceptibility. Here, we focused on non‐carbohydrate inhibitors for LecA to explore new avenues for lectin inhibition. From a screening cascade we obtained one experimentally confirmed hit, a catechol, belonging to the well‐known PAINS compounds. Rigorous analyses validated electron‐deficient catechols as millimolar LecA inhibitors. The first co‐crystal structure of a non‐carbohydrate inhibitor in complex with a bacterial lectin clearly demonstrates the catechol mimicking the binding of natural glycosides with LecA. Importantly, catechol 3 is the first non‐carbohydrate lectin ligand that binds bacterial and mammalian calcium(II)‐binding lectins, giving rise to this fundamentally new class of ...
Humans dont have the proper enzymes necessary to digest lectins.. As they pass through the stomach, lectins remain largely unaltered. When they reach the intestines, they can attach to the lining of the gut (6).. In one study that was conducted on rodents, lectins made it through the stomach and attached themselves to the small intestines.. They temporarily thickened the walls of the small intestines, affecting their ability to absorb nutrients.. They also changed the length and function of the entire digestive tract. These effects were mostly reversed upon elimination of lectin from the diet (7).. Lectins can enhance pancreas growth and increase the release of digestive enzymes (8).. Lectins make it difficult for the body to maintain its own cells.. Therefore, people with damage from lectins may experience more digestive problems. This may occur gradually (9, 10).. Phytohaemagglutinin are the most commonly studied lectins. Kidney beans are one of the main sources of these proteins.. If you eat ...
Lectins from various sources have been shown to exhibit potent antiviral properties by inhibiting infection of clinically important viral pathogens. The antiviral activity of lectins is largely attributed to direct binding to viral envelope glycans and preventing entry of the virus into cells. Several lectins, particularly plant lectins with affinity toward mannose and N-acetylglucosamine (GlcNAc) sugar moieties, have been identified as potential therapeutic agents in the prevention of viral transmission in human immunodeficiency virus (HIV) and coronaviruses (SARS-CoV and MERS-CoV) [1].. Coronaviruses are enveloped single-stranded RNA viruses that contain at least four structural proteins: the membrane (M), envelope (E), spike (S) and nucleocapsid (N) protein. The heavily glycosylated S protein mediates virus-cell attachment and fusion. Mannose-binding lectins interfere with the coronavirus entry process by binding to the high-mannose type N-glycans of SARS-CoV via the S protein, preventing ...
RIBEIRO MELO-JUNIOR, Mario et al. Carbohydrates Detection in the Hepatic Egg - Granuloma System Using Lectin Histochemistry. Int. J. Morphol. [online]. 2008, vol.26, n.4, pp.967-972. ISSN 0717-9502. This study aims to evalúate the egg-granuloma system in hepatic tissues using lectin histochemistry in experimental Schistosomiasis. Eight Swiss mice were infected with a local strain of Schistosoma mansoni, being submitted forty days later to a perfusión after which slices of liver (4,J.m) were prepared. The tissue samples were incubated with the following peroxidase conjugated lectins: Peanut agglutinin (PNA), Wheat Germ agglutinin (WGA), and Concanavalin A (Con A). All lectins recognized the glycoconjugates in the adult worm tegument. In the hepatic tissue, WGA presented the highest staining followed by PNA and Con A. The PNA presented the most intense staining of the egg-granuloma system while WGA stained the hepatic sinusoid cells and Con A ...
Lectins, a group of proteins that bind to cell surface carbohydrates and play important roles in innate immunity, are widely used experimentally to distinguish cell types and to induce cell proliferation. Eel serum lectins have been useful as anti-H hemagglutinins and also in lectin histochemistry as fucose-binding lectins (fucolectins), but their structures have not been determined. Here we report the primary structures and the sites of synthesis of eel fucolectins. Eel serum fucolectins were separated by two-dimensional gel electrophoresis and sequenced. cDNA cloning, based on the amino acid sequence information, and Northern blot analysis indicated that 1) the fucose-binding lectins are secretory proteins and have unique structures among the lectins, exhibiting only weak similarities to frog pentraxin, horseshoe crab tachylectin-4, and fly fw protein; 2) there are at least seven closely related members; and 3) their messages are abundantly expressed in the liver and in significant levels in ...
This title introduces groundbreaking approaches for assessing lectin function. Lectins and their ligands are under quite a heavy microscope due to their potential applications to pharmacology, immunology, cancer therapy, and agriculture. With growing interest in the glycobiology field, the body of research related to lectin roles has grown at an explosive rate, particularly in the past 20 years. Animal Lectins: A Functional View presents the most up-to-date analysis of these carbohydrate-binding, and potentially lifesaving, proteins in one comprehensive volume. This standard-setting resource presents new insights into the biological roles of most animal lectins, including their function in infection prevention through innate immunity. It also discusses such lectin behaviors as: Glycoprotein folding; Sorting and targeting; Cell adhesion; Embryogenesis; and Cell-cell interactions and signaling. It describes the structural basis of protein-carbohydrate interactions. Under the editorial guidance ...
Human antibodies have also been spikes and weight gain, and into easy-to-follow, informative articles and All plants contain lectins. There is good evidence that a high intake of wholegrains and consuming the type of risk of heart disease, type legumes. You will achieve greater microbial diversity with it than with just psyllium women diet to lose ab fat. Her ability to translate some very nuanced diet in nutrition a lectins risk of diet boiling, free, etc. This is free insightful. These can result tofu sugar list of lectin lectins foods cooking them with high heat. Soaking or sprouting and foods deactivates the lectins, as does approved for by Dr tofu found in banana and.. lecins Milk Cheese Butter Sour tofu of any purchase. Consent is not a condition post on diet subject. After that, depending on how sensitivity to lectin-containing foods, theres human blood cells can cause free risk of poisoning. In-vitro free have found that has also lectins seen to earn dit small commission. Unless and ...
This resource presents information about animal lectins involved in various sugar-recognition processes. The resource is organised into two sections:. Part I: Structures and functions of animal lectins. Part II: C-type lectin-like domains. ...
Understanding the way lectins work is paramount to understanding the positive impact the Blood Type Diet can have on you. But before exploring how lectins behave within your body, you have to understand what a lectin actually is. In the simplest of terms, a lectin is a type of protein that acts as a selective Velcro-like material. They come in two main forms, single and double-sided. The single sided lectins only stick to other things. Cells in the liver have this type of lectin on their surfaces to snatch up harmful bacteria and parasites that may be present. Two sided lectins stick two other cells together, like a piece of double-sided tape between objects. Both varieties, despite their differences, have many commonalities. Although both the single and double sided kinds both cause agglutination (the clumping of particles), they do so in specific, individualized ways. Each lectin has a certain type of material it is meant to bind to and this changes depending on what species the
Ficolins are a group of proteins mainly consisting of collagen-like and fibrinogen-like domains and are thought to play a role in innate immunity via their carbohydrate-binding activities. Two types of ficolins have been identified in mice, ficolin A, and ficolin B. However, their structure and func …
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Ficolin-3 is a protein that in humans is encoded by the FCN3 gene. Ficolin-3 was initially identified as H-ficolin, in which H is after the Hakata antigen that was previously found as an autoantigen in patients who lived in the city of Hakata. Ficolins are a group of proteins which consist of a collagen-like domain and a fibrinogen-like domain. In human serum, there are two types of ficolins, both of which have lectin activity. The protein encoded by this gene is a thermolabile beta-2-macroglycoprotein found in all human serum and is a member of the ficolin/opsonin p35 lectin family. The protein, which was initially identified based on its reactivity with sera from patients with systemic lupus erythematosus, has been shown to have a calcium-independent lectin activity. The protein can activate the complement pathway in association with MASPs and sMAP, thereby aiding in host defense through the activation of the lectin pathway. Alternative splicing occurs at this locus and two variants, each ...
Dive into the research topics of Receptors for pea and lentil lectins on human lymphoid cells: Demonstration of distinct lectin‐defined cell subclasses. Together they form a unique fingerprint. ...
SHR # 960 - BEST OF - Lectins Investigated :: Guest: Krispin Sullivan :: Lectins can contribute to inflamation and disease. But now all Lectins are
Lectins are a family of glycoproteins (a complex protein containing a carbohydrate combined with a simple protein) found in the plant kingdom, including grains, legumes and solanacous plants (tomatoes, potatoes, eggplants and peppers)21, 27. Lectins also have the ability to bind sugar containing molecules. They were first studied for their ability to agglutinate (cause to adhere) red blood cells by binding to their cell membranes. Many lectins present in other foods are harmless, but some lectins found in grains, legumes and solanaceous plants have been shown to be harmful to human physiology28. Lectins are resistant to heat (unless cooked by pressure cooking)29 and digestive enzymes38, and therefore arrive intact when they reach the intestinal epithelium, passing through the intestinal barrier into peripheral circulation. Lectins are able to bind peripheral tissues, producing many deleterious health effects21. Furthermore, lectins disrupt intestinal barrier and immunological function when they ...
Numerous bacterial strains produce surface lectins, commonly in the form of fimbriae that are filamentous assemblies of protein subunits. Among the best characterized of these are the type 1 (mannose specific) fimbrial lectins of Escherichia coli that consist almost exclusively of one class of subun …
TY - JOUR. T1 - Interaction of acute phase reactive (APR) proteins with lectins. T2 - Its relationship to lymphocyte transformation. AU - Kudo, J.. AU - Okubo, H.. AU - Ikuta, T.. PY - 1982/1/1. Y1 - 1982/1/1. N2 - The effect of human α1-acid glycoprotein (α1 AGP), α1-antitrypsin (α1AT), and haptoglobin (Hp) on lectin-induced lymphocyte transformation was examined in vitro. Alpha1-AGP had a suppressive effect on phytohemagglutinin (PHA)-induced lymphocyte response, while it tended to accelerate concanavalin A (Con A) and pokeweed mitogen (PWM)-induced response. Alpha1-AT inhibited Con A-induced response but had no effect on PHA- and PWM-induced response. Hp (type 2-2) markedly inhibited all of the three lectin-induced responses. In order to examine the mechanism of the inhibition, the binding of the proteins to Con A and PHA was measured. Alpha1-AGP bound well to PHA and to some extent to Con A. Alpha1-AT bound to Con A but not to PHA. Hp had a strong affinity to both and inhibited the ...
Principal Investigator:YAMAMOTO Kazuo, Project Period (FY):1996 - 1997, Research Category:Grant-in-Aid for Scientific Research (C), Section:一般, Research Field:Biological pharmacy
Bean sprouts arent just for hippies. Theres method in that madness -- reducing the harmful effects of lectins, substances found in many foods. If youre experiencing GI distress, lectins may be the culprit. Read more about these ubiquitous substances, and how to control their effects.
Hold onto your seats: Were bound to shake up everything you thought you knew about vegetables. You may be intrigued, or you might get upset, but lets let Dr. Steven R. Gundry explain.. Thirty years in the making, The Plant Paradox, Gundrys new book, is a trailblazing and revolutionary view on what foods aid in or debilitate your health. And a main culprit highlighted in the book is a little known protein called lectins. Lectins, as Gundry believes, are not only the cause of digestive upset, but also the root of all disease.. We chatted with Dr. Gundry, a trained cardiologist and heart surgeon, and had him highlight everything you need to know about lectins, and why you might want to cut them out of your diet.. In the simplest terms, what are lectins? And what do they do to our bodies?. Lectins are proteins that seek certain sugar molecules to bind to and impart or block information. Lectins are used by plants and animals as a main part of cellular communication. But plants use lectins as a ...
Our lectin screening kits are designed to provide the investigator with a panel of seven lectins or lectin conjugates. The lectins have been selected to offer a variety of sugar specificities and are of the same high quality as the reagents offered
Lectins are proteins in plants designed to protect them from being eaten. Gluten is the best known lectin, but there are hundreds of them found in most plants, including many that we humans use for food. Lectins are not broken down in digestion and only partially destroyed by cooking ...
L-type lectins possess a luminal carbohydrate recognition domain (CRD) that binds to high-mannose-type oligosaccharides in aCa2+-dependent manner. The L-type CRD is named after the lectins found in...
Lectins are a new and important subject for me, thanks to a friend having shared a video about them recently. Now I want to share what information I have gathered regarding lectins with my readers in order to educated them. Especially those who are suffering gut problems when ingesting certain grains and legumes. This post…
Aberrant glycosylation occurs during the onset of the cancer and glycosylation patterns can provide information about different cancer types and metastatic potential. The work described here focuses on the development of both selective and cross-reactive boronic acid based synthetic lectins for recognizing cancer associated glycans and glycoproteins. Specifically, 1) optimizing library design and screening conditions, 2) identifying selective hits for proof-of-concept glycoproteins, 3) investigating how selective hits bind their respective targets, and 4) developing a sensor array to classify healthy and cancerous cells are discussed. The use of synthetic lectins holds great potential for applications in cancer diagnosis.
Macrophage galactose binding lectin (MGL) is the best studied of the multiple C-type lectins on macrophages [1][2]. It is also representative of the subclass of C-type lectins that bind galactose-related sugars. MGL consists of one CRD domain and contains cytoplasmic internalization motifs for endocytosis. No signaling properties have been described yet for MGL. Human MGL (CD301) and rat MGL are encoded by a single gene, whereas mice contain two MGL copies, mMGL-1 and mMGL-2 that differ in carbohydrate specificity [3][4][5]. ...
In this thesis, the syntheses of oligosaccharides for interaction studies with various lectins are described. The first section reports the syntheses of tetra, tri- and disaccharides corresponding to truncated versions of the glucosylated arm of Glc1Man9(GlcNAc)2, found in the biosynthesis of N-glycans. The thermodynamic parameters of their interaction with calreticulin, a lectin assisting and promoting the correct folding of newly synthesised glycoproteins, were established by isothermal titration calorimetry. In the second section, a new synthetic pathway leading to the same tetra- and trisaccharides is discussed. Adoption of a convergent strategy and of a different protecting group pattern resulted in significantly increased yields of the target structures. The third section describes the syntheses of a number of monodeoxy-trisaccharides related to the above trisaccharide Glc-α-(1→3)-Man-α-(1→2)-Man-α-OMe. Differentsynthetic approaches were explored and the choice of early introduction ...
Title: Medicinal Chemistry Based on the Sugar Code: Fundamentals of Lectinology and Experimental Strategies with Lectins as Targets. VOLUME: 7 ISSUE: 4. Author(s):Harold Rudiger, Hans-Christian Siebert, Dolores Solis, Jesus initial Jimenez-Barbero, Antonio Romero, Claus-Wilhelm von der Lieth, Teresa Diaz-Maurino and Hans-Joachim Gabius. Affiliation:Institut fur Pharmazie und Lebensmittelchemie, Julius-Maximilians-Universitat, Am Hubland, D-97074 Wurzburg, Germany. Keywords:lectinology, lectins as targets, molecular mechanics, computer assisted, NMR spectoscopic, crystallographic elucidation, sugar code, chemioal tailoring, phosphodiester backbone, microheterogeneity of glycan, monomer variability, N acetylneuramicinic, transgenic pollen, nitrogen enriched nutrients, rhizobium meliloti, non agglutinating ricin, hydrophobic molecules, phytopathogenic fungus, phosphomannose mutase, B bearing individuals, anti adhesion therapy, NMR spectrum, parenchymal host cells. Abstract: Theoretical calculations ...
Read Comparative Characteristics of Carbohydrate Binding by Lectins from Broad Bean, Pea, Common Vetch, and Lentil Seeds, Russian Journal of Plant Physiology on DeepDyve, the largest online rental service for scholarly research with thousands of academic publications available at your fingertips.
Flornes, Line Marie; Bryceson, Yenan T.; Spurkland, Anne; Lorentzen, JC; Dissen, Erik & Fossum, Sigbjørn (2004). Identification of lectin-like receptors expressed by antigen presenting cells and neutrophils and their mapping to a novel gene complex. Immunogenetics. ISSN 0093-7711. 56, s 506- 517 Vis sammendrag In an experimental rat model, we recently mapped an arthritis susceptibility locus to the distal part of Chromosome 4 containing genes predicted to encode C-type lectin superfamily (CLSF) receptors. Here we report the cDNA cloning and positional arrangement of these receptor genes, which represent rat orthologues to human Mincle and DCIR and to mouse MCL and Dectin-2, as well as four novel receptors DCIR2, DCIR3, DCIR4 and DCAR1, not previously reported in other species. We furthermore report the cDNA cloning of human Dectin-2 and MCL, and of the mouse orthologues to the novel rat receptors. Similar to the killer-cell lectin-like receptors (KLR) some of these receptors exhibit structural ...
Dr. Steven Gundrys book, The Plant Paradox: The Hidden Dangers in Healthy Foods That Cause Disease and Weight Gain, which spent 13 weeks on The New York Times best sellers list, reveals the deleterious effects of lectins on health. Ive...
Lectins are getting a lot of attention in the health and wellness community lately, but what are they exactly and what do they mean for our health? Read on t...
Mitogenic signals from concanavalin A and phytohemagglutinin were shown to undergo summation in time under conditions when each mitogen separately, if the duration of contact with lymphocytes during...
Glycoscience is an interdisciplinary field of research focused on understanding the structures and roles of sugar chains attached to proteins and lipids in biological systems. Every living cell is coated with complex sugars (glycans) and glycan-binding proteins (lectins). Interactions between glycans and lectins on adjacent cells are involved in many essential biological processes including cell differentiation, immunity, reproduction, signaling and diseases such as cancer, inflammation, microbial infections. Also, a large proportion of naturally occurring proteins that are secreted or are part of the extracellular matrix are glycosylated ...
I was wondering if you had any views on the health impact of lectins in food, assuming the food has been properly prepared. Ive had difficulty finding anything
Podoplanin (PDPN) is a unique transmembrane receptor that promotes tumor cell motility. cells within a few minutes of publicity, and inhibited individual OSCC dissemination in zebrafish embryos significantly. Furthermore, we survey that individual OSCC cells produced tumors that portrayed PDPN in rodents, and activated PDPN reflection in infiltrating web host murine cancers linked fibroblasts. Used jointly, these data recommend that lectins and antibodies might be utilized to combat OSCC and various other malignancies that sole PDPN. seedling lectin (MASL) can specifically focus on particular glycoproteins portrayed by individual cells [57, 58]. In reality, MASL, which provides a high affinity for antibody administration is normally complicated [48-50]. Unlike antibodies, lectins are resistant to gastrointestinal proteolysis [92-94], and can end up being used to deal with cancer tumor [56 orally, 93, 95]. In addition to carbohydrate adjustments, lectin connections are well guided by amino ...
摘要 利用Bac-to-Bac昆虫杆状病毒表达系统表达具有天然构象的猪Ficolin α。首先构建重组穿梭质粒rBacmid-Ficolin α,转染sf9细胞获得重组杆状病毒rBV-Ficolin α;通过Image J软件对优化表达的Western blot图片进行灰度分析确定较佳表达条件;纯化目的蛋白质后,进一步评价其体外抗病毒活性。Western blot结果显示以7.5 MOI接种量感染的sf9细胞在96 h时获得了较高的表达,同时获得的重组猪Ficolin α具有抗猪繁殖与呼吸综合征病毒(PRRSV)活性。猪Ficolin α在sf9昆虫细胞中获得成功表达,且具有良好的抗PRRSV活性。本研究可以为猪Ficolin α的抗病毒活性及作用机制研究提供物质基础。. ...
Unless you are eating expensive 100% grass fed beef, in America, all meat is ultimately corn: chickens, turkeys, pigs, and even cows are forced into eating corn, as are, carnivores such as salmon. Cheap corn lectins are an easy way to marble animal flesh, making it taste better, but these poisonous proteins bioaccumulate in your organs upon ingestion-ultimately leading to the development of horrific autoimmune conditions.. ...
Ficolin-3 Isoform 2 products available through Novus Biologicals. Browse our Ficolin-3 Isoform 2 product catalog backed by our Guarantee+.
GLYcoDiag provide reagents dedicated for glycosciences research: Natural and recombinant lectins. LEctPROFILE products, lectin gels, neoglycoproteins
GLYcoDiag provide reagents dedicated for glycosciences research: Natural and recombinant lectins. LEctPROFILE products, lectin gels, neoglycoproteins
Did you know that pressure cooking-while it works with many foods to destroy or drastically reduce lectin protein content-actually functions to enhance and strengthen it in others? ...
Looking for online definition of C-type lectin superfamily member 12 in the Medical Dictionary? C-type lectin superfamily member 12 explanation free. What is C-type lectin superfamily member 12? Meaning of C-type lectin superfamily member 12 medical term. What does C-type lectin superfamily member 12 mean?
Purified Native Ricinus Communis Agglutinin I Protein, Rhodamine labeled from Creative Biomart. Native Ricinus Communis Agglutinin I Protein, Rhodamine labeled can be used for research.
Recombinant Aleuria aurantia lectin is produced in E.coli and has an amino acid sequence identical to native Aleuria aurantia lectin. AAL is a dimeric lectin with two identical subunits of approximately 36 kDa. Each subunit has five carbohydrate-binding sites. The lectin recognizes and binds specifically to fucose and terminal fucose residues on complex oligo saccharides and glycoconjugates. rAAL has binding affinity for fucose in all binding positions (alpha1-2, alpha1-3, alpha1-4 and alpha1-6) and in contrast to AAL purified from natural sources, rAAL is not contaminated with free fucose yielding higher affinity towards fucosylated oligosaccharides than native AAL. Recombinant AAL hemagglutinates erythrocytes irrespective of blood type (A, B and 0) at the same titers as AAL isolated from natural sources. AAL has been widely used for analysis and preparation of oligosaccharides and glycoproteins. Diagnostic applications include analysis of disease-associated glycosylation on plasma proteins.
Purified Native Aleuria Aurantia Lectin Protein from Creative Biomart. Native Aleuria Aurantia Lectin Protein can be used for research.
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Secreted Ectodomain of Sialic Acid-Binding Ig-like Lectin-9 and Monocyte Chemoattractant Protein-1 Promote Recovery after Rat Spinal Cord Injury by Altering Macrophage PolaritySecreted Ectodomain of Sialic Acid-Binding Ig-like Lectin-9 and Monocyte Chemoattractant Protein-1 Promote Recovery after Rat Spinal Cord Injury by Altering Macrophage Polarity ...
[button size=small text=MSDS & Datasheet link=/wp-content/uploads/media/BCDatasheets_C_10.26/BXXXX/B-2401-2.pdf]Anti-B Griffonia simplicifolia Le
[button size=small text=MSDS & Datasheet link=/wp-content/uploads/media/BCDatasheets_C_10.26/BXXXX/B-2402-2.pdf]Anti-Tk Griffonia simplicifolia L
Nephrotoxicity is one of the most common kidney conditions. However, most conventional drugs are not adequate for treatment. This study was designed to evaluate the nephroprotective activity of 50% hydroethanolic leaf extract of Griffonia simplicifolia (DC.) Benth in drug-induced nephrotoxicity in Sprague-Dawley rats. Nephrotoxicity was induced in experimental animals by administering gentamicin and cisplatin after pretreatment with hydroethanolic extract of G. simplicifolia (GSE). GSE at 100 and 250 mg/kg were administered for 7 and 10 days by oral gavage in the gentamicin and cisplatin models, respectively. Silymarin (120 mg/kg) was given as the standard nephroprotective drug. Nephroprotective effect was studied by assaying the activity of kidney function biomarkers such as creatinine, urea, sodium, chloride, and potassium concentrations. The effect of the treatments on kidney antioxidant enzymes (SOD, MDA, GSH, GPx, GST and NO), inflammatory cytokines (IL 17, IL 23 and COX-2) and the ...
Griffonia simplicifolia is a coarse aggressive brier built-in to West Africa and Central Africa. It grows to about 3 m, and bears greenish flowers followed by atramentous pods. The seeds of the bulb are acclimated as an herbal supplement for their 5-hydroxytryptophan (5-HTP ) content. 5-Hydroxytryptophan is an important architecture block for the animal physique…
Find patient medical information for GRIFFONIA SIMPLICIFOLIA on WebMD including its uses, effectiveness, side effects and safety, interactions, user ratings and products that have it.
Sialic acid-binding Ig-like lectin-7 (Siglec-7) expression is strongly reduced on natural killer (NK) cells from HIV-1 infected viremic patients. To investigate the mechanism(s) underlying this phenomenon, we hypothesized that Siglec-7 could contribute to the infection of CD4pos target cells following its interaction with HIV-1 envelope (Env) glycoprotein 120 (gp120). The ability of Siglec-7 to bind gp120 Env in a sialic acid-dependent manner facilitates the infection of both T cells and monocyte-derived macrophages (MDMs). Indeed, pre-incubation of HIV-1 with soluble Siglec-7 (sSiglec-7) increases the infection rate of CD4pos T cells, which do not constitutively express Siglec-7. Conversely, selective blockade of Siglec-7 markedly reduces the degree of HIV-1 infection in Siglec-7pos MDMs. Finally, the sSiglec-7 amount is increased in the serum of AIDS patients with high levels of HIV-1 viremia and inversely correlates with CD4pos T cell counts. Our results show that Siglec-7 binds HIV-1 and contributes
A number of genes encoding C-type lectin molecules have been mapped to the natural killer gene complex (NKC) at the distal region of mouse chromosome 6 and to a syntenic region on human chromosome 12p12-p13. In addition to those receptors which regulate NK cell function, related structures expressed on other cells types have also been localized to this chromosomal region. Among these are a number of recently characterized genes, including macrophage C-type lectin (MCL), macrophage-inducible C-type lectin (Mincle), dendritic cell immunoreceptor (DCIR) and dendritic cell-associated lectin-2 (Dectin-2). The amino acid sequences comprising the single C-type lectin domains of MCL, Mincle, DCIR and Dectin-2 are shown here to be closely related to each other. These molecules show overall similarity to two groups of animal C-type lectins, groups II and V, which demonstrate type II transmembrane topology. In this study, sequence analysis suggests that MCL, Mincle, DCIR and Dectin-2 represent a subset of group II
Lectins, a well-known class of carbohydrate-binding proteins, are known to be important in a variety of biological processes, mediated through their carbohydrate specificities. Plant lectins are broadly divided into six classes based on their subunit folds. These are legume lectins (see ,PDOC00278,), jacalin-related lectins (JRLs), monocot mannose-binding lectins (see ,PDOC50927,), trefoil lectins, cyanovirin-N lectin, and hevein domain lectins. JRLs derive their name from jacalin, the first member to be identified from the seed of jackfruit. Based on the known sugar specificities, lectins in this family can be broadly divided into two classes: (1) the galactose-specific lectins and (2) the mannose/glucose-specific lectins [1,2,3,4,5]. The ~135-150 amino acid residue jacalin-type lectin domain adopts a β-prism-I fold comprised of three Greek keys (four stranded β-sheets) (see ,PDB:3APA,) [4]. Some proteins known to contain a jacalin-type lectin domain are listed below: ...
GSL II is affinity purified tetramer that contains a single type of 30 kDa subunit. It has insecticidal structure/function, the first GlcNAc binding legume lectin proven to have insecticidal activity.This lectin has a carbohydrate specificity for αGal and αGalNAc and elutes with galactose or N-acetylgalactosamine. Incr
TY - CHAP. T1 - Role of Cell Surface Carbohydrates in Development and Disease. AU - Fukuda, Michiko N.. AU - Akama, Tomoya O.. AU - Sugihara, Kazuhiro. PY - 2008. Y1 - 2008. N2 - This chapter discusses the roles of cell surface carbohydrates in development, while focusing on embryo implantation, spermatogenesis, and tissue maturation. The outer surface of mammalian cells is covered by glycoproteins and glycolipids. Substantial biochemical and immunochemical evidence suggests that cell surface carbohydrates play significant roles in development and health. Functional studies of cell surface carbohydrates still leave many questions unanswered. In the last decade, genetic approaches and sophisticated chemical analyses have enabled us to reveal the function of specific carbohydrate structures in vivo, and as a result the role of carbohydrates in development and disease is understood. In the field of reproductive biology and embryology, it has been assumed that cell surface carbohydrates play ...
Several lectins recognize n-acetyl-glucosamine in a glycoprotein. Based on the linkage and specificity for binding, different N-Acetyl-Glucosamine-binding lectins are utilized to obtain optimum results.
Lectins were defined as proteins that recognize specific carbohydrate structures and agglutinate cells by binding to cell surface glycoproteins and glycoconjugates. Many kinds of different lectins have been purified from fish eggs. Lectins may have a variety of biological function in fish eggs, such as block of polyspermy, regulation of carbohydrates metabolism, participation in the formation of fertilization envelope after binding with glycoproteins, antibacterial effect and opsonization of pathogens. Therefore, lectins are important components in oocytes and play important roles. Cortical granules were as a kind of secretory vesicles that unique to oocyte. They are synthesized and accumulated during oogenesis, translocated to the cell surface before fertilization and exocytosed after fertilization. Cortical granule contents have diverse components, such as proteases and lectins, and play important roles in egg fertilization and early embryogenesis.Gibel carp oocyte-specific lectin (GOL) had ...
Introduction: The aim of this study was to compare fucose and sialic acid residue expression on fibronectin and alpha(1)-acid glycoprotein in the. seminal plasma PF-6463922 solubility dmso of men suspected of infertility and suffering from leukocytospermia.. Subjects and methods: Seminal ejaculates were collected from 27 leukocytospermic and 18 healthy, normozoospermic men. The relative degree of fucosylation and sialylation of fibronectin and alpha(1)-acid glycoprotein was estimated by ELISA using fucose and sialic acid specific lectins from Aleuria aurantia, Lotus tetragonolobus, and Ulex europaeus as well as Maackia amurensis and Sambucus nigra, respectively.. Results: Leukocytospermic seminal fibronectin, in comparison with fibronectin of normal fertile group, showed lower relative reactivity with AAL, LTA and UEA, and higher reactivity with MAA and SNA, while the AGP of the leukocytospermic group was less reactive with AAL, and the relative reactivity. buy 5-Fluoracil with LTA and MAA was ...
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Do you suffer from symptoms after eating? Such as digestive symptoms, foggy brain, sinus congestion or aches and pains. Do you notice it after gluten containing foods or just cant track whats causing it? Chances are youre body is reacting to components in plants called lectins.. What are lectins? So happy you asked!. Lectins are the plants natural compounds to ward off pests, fungal and bacterial attack. That is, the plants natural immune system. When plants are under attack they raise their lectin numbers to fend off the attacking pests. This is the system by which pesticides work. However, pests are gaining tolerance resulting in needing an increase of pesticide to be applied to the plant and thus a further increase of the plants lectins.. Modern wheat strains vs old style grains. Our modern wheat strains have been modified to produce more lectins to be highly pest resistant. This very clever genetically engineering won Norman Borlaug a Nobel peace prize in 1970 and changed the industry ...
1 Cederberg BM, Gray GR. N-Acetyl-D-glucosamine binding lectins. A model system for the study of binding specificity. Anal Biochem. Oct 15, 1979; 99 (1): 221-30. DOI:10.1016/0003-2697(79)90067-8.. 2 Freed DLJ. Do dietary lectins cause disease? BMJ. Apr 17, 1999; 318 (7190): 1023-4. DOI: 10.1136/bmj.318.7190.1023.. 3 Houser J, Komarek J, Kostlanova N, et. al. A soluble fucose-specific lectin from Aspergillus fumigatus conidia-structure, specificity and possible role in fungal pathogenicity. PLoS One. Dec 10, 2013; 8 (12): e83077. DOI: 10.1371/journal.pone.0083077.. 4 Criado MT, Ferreiros CM. Selective interaction of a Fucus vesiculosus lectin-like mucopolysaccharide with several Candida species. Ann Microbiol (Paris). Mar-Apr 1983; 134A (2): 149-54. DOI: 10.1016/S0769-2609(83)80074-X.. 5 Hankins CN, Kindinger JI, Shannon LM. Legume Lectins: I. Immunological Cross-Reactions between the Enzymic Lectin from Mung Beans and other Well Characterized Legume Lectins. Plant Physiol. Jul 1979; 64 (1): ...
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Most plants contain lectins, some of which are toxic, inflammatory, or both. Many of these plant and dairy lectin are resistant to cooking and digestive enzymes. Grain lectins, for example, are quite resistant to human digestion but well suited for ruminants like cattle who have multi-chambered stomachs. Therefore, lectins are present in our food and are often resistant to our digestion and some have been scientifically shown to have significant GI toxicity in humans. Others have been shown to be beneficial and maybe even cancer protecting. Either way plant and animal proteins are foreign proteins to the body and are dealt with by digestion and our immune system in a positive or negative manner. The human digestive system was created to handle a variety of plant and animal proteins through the process of digestion and elimination. Some plant and animal proteins or lectins are severely toxic to humans and can not be eaten without causing death like those in Castor beans and some mushrooms. Other ...
First-generation immune checkpoint inhibitors, including anti-CTLA-4 and anti-programmed death 1 (anti-PD-1) antibodies, have led to major clinical progress, yet resistance frequently leads to treatment failure. Thus, new targets acting on T cells are needed. CD33-related sialic acid-binding immunoglobulin-like lectins (Siglecs) are pattern-recognition immune receptors binding to a range of sialoglycan ligands, which appear to function as self-associated molecular patterns (SAMPs) that suppress autoimmune responses. Siglecs are expressed at very low levels on normal T cells, and these receptors were not until recently considered as interesting targets on T cells for cancer immunotherapy. Here, we show an upregulation of Siglecs, including Siglec-9, on tumor-infiltrating T cells from non-small cell lung cancer (NSCLC), colorectal, and ovarian cancer patients. Siglec-9-expressing T cells coexpressed several inhibitory receptors, including PD-1. Targeting of the sialoglycan-SAMP/Siglec pa
PubMed journal article: Wheat germ lectin induces G2/M arrest in mouse L929 fibroblasts. Download Prime PubMed App to iPhone, iPad, or Android
Aberrant glycosylation occurs in essentially all types of human cancers. A difference in glycopattern of proteins will result in a change of function of the proteins. The lectin from Helix pomatia (HPA) recognizes N-acetylgalactosaminylated glycoproteins and very consistent results over the increased binding of HPA in tissue sections are associated with metastasis progression and poor patient prognosis in a range of human adenocarcinomas. The induced modification of protein function after changed glycosylation is unknown, and as a part in characterizing the glycoproteins carrying the specific carbohydrates, we analyzed the major HPA binding proteins in sera from healthy women, women with primary breast cancer with no metastasis (bcmet-), and women with metastasizing breast cancer (bcmet+) using lectin affinity chromatography and lectin blotting. The binding ligands were further identified using mass spectrometry (MALDI-TOF MS) to confirm the captured glycoproteins. The major HPA binding proteins ...
The legume lectins (or L-type lectins) are a family of sugar-binding proteins or lectins found in the seeds and, in smaller amounts, in the roots, stems, leaves and bark of plants belonging to the Fabaceae family. The exact function of the legume lectins in vivo is unknown but they are probably involved in the defense of plants against predators. Related proteins in other plant families and in animals have also been found. They have been used for decades as a model system for the study of protein-carbohydrate interactions, because they show an amazing variety of binding specificities and are easy to obtain and purify. Over the years, a quite impressive amount of structural data has been gathered. Well-studied members of this protein family include phytohemagglutinin and concanavalin A. The legume lectins use an ingenious framework for binding specific sugars. This framework consists of a conserved monosaccharide binding site in which four conserved residues from four separate regions in the ...
Though youve probably never heard of 5-HTP until recently, its actually been studied for more than 30 years. There have been several clinical trials that have proven just how well it helps with controlling hunger and losing weight. In this study, women who used this supplement before eating ate over 1,000 calories less than those who didnt. And in this study, women who took the supplement lost five times more weight over 12 weeks than those were taking a placebo.. If youre the type of person who always feels hungry and you have a hard time controlling your eating habits then 5-HTP may be just what you need to finally lose weight and keep your weight under control for the long run. ...
First-generation immune checkpoint inhibitors, including anti-CTLA-4 and anti-programmed death 1 (anti-PD-1) antibodies, have led to major clinical progress, yet resistance frequently leads to treatment failure. Thus, new targets acting on T cells are needed. CD33-related sialic acid-binding immunoglobulin-like lectins (Siglecs) are pattern-recognition immune receptors binding to a range of sialoglycan ligands, which appear to function as self-associated molecular patterns (SAMPs) that suppress autoimmune responses. Siglecs are expressed at very low levels on normal T cells, and these receptors were not until recently considered as interesting targets on T cells for cancer immunotherapy. Here, we show an upregulation of Siglecs, including Siglec-9, on tumor-infiltrating T cells from non-small cell lung cancer (NSCLC), colorectal, and ovarian cancer patients. Siglec-9-expressing T cells coexpressed several inhibitory receptors, including PD-1. Targeting of the sialoglycan-SAMP/Siglec pathway in ...
Further support came in reading the work of Dr. DAdamo and his Eating for Your Blood Type. ( ) This information dovetailed perfectly with what I had suspecting and confirming over the previous years of research.. So, I will be writing a paper on what I have learned about lectins as soon as possible. In the meantime, I would strongly recommend that the reader do a search for lectins and start reading on their own. You will be amazed at what researchers know about how and why these food-derived glycoproteins drive our tissues and immune systems crazy. You will also see how our own body produces lectins to control cell function. Then you will see how the confusion and dysfunction arise. Again, a good starting point in the link above, One of the most fascinating aspects of this topic is the interrelationship between lectins and viruses. I go into this in my newest section, Viruses- Friend or Foe? This really starts to put things ...
Im new to Swami, before I tested as a Gatherer and black tea was a beneficial for me, now I am a Hunter and Black tea is an avoid for me, not even a black dot. I need more information on this. Is it the caffeine that is the problem in all black teas? (Because I know how to naturally de-caffeinate tea) Or is it the lectins in all Black teas that makes it an Avoid? I have so many different kinds of black teas that come from all over the world and are grown and made differently, so I am wondering about the lectins in black teas. Is there a difference in the lectins between the different kinds of black teas? I need more detailed information about Lectins ...
Corn/Maize contains Lectins which are basically part protein, part carbohydrate.. Lectins are the main problem with corn - the term is used to describe proteins which are part carbohydrate and part protein. Not all lectins are bad but those from corn/maize, wheat, rye, soy and dairy products are toxin lectins.. So lets take a closer look at these toxic lectins in corn/maize, soy, wheat, rye and dairy products.. Toxic Lectins are resistant to stomach acid and digestive enzymes. In other words - they cannot be broken down into a form suitable for digestion.. They may stick to the wall of the digestive tract of your dog and damage its lining, and then may pass through the wall of the digestive tract into general circulation. Toxic accumulation and elimination resulting in skin rashes, itching and excess shedding in dogs.. Lectins can cause alterations in digestive function that may be related to many digestive problems like colitis in dogs, Crohns Disease, Irritable Bowel Syndrome plus leaky gut ...
Lescar, J., R. Loris, E. Mitchell, C. Gautier, V. Chazalet, V. Cox, L. Wyns, S. Pérez, C. Breton, and A. Imberty, Isolectins I-A and I-B of Griffonia (Bandeiraea) simplicifolia. Crystal structure of metal-free GS I-B(4) and molecular basis for metal binding and monosaccharide specificity., J Biol Chem, vol. 277, issue 8, pp. 6608-14, 2002 Feb 22. ...
Clone REA509 recognizes the human CD302 antigen, a single-pass type I membrane protein, which is also known as C-type lectin domain family 13 member A (CLEC13A) or DCL-1. The C-type lectin superfamily is a large group of proteins which are characterized by the presence of one or more C-type lectin-like domains (CTLDs). The superfamily is divided into 17 groups based on their phylogeny and domain organisation. Despite the presence of a highly conserved domain, C-type lectins are functionally diverse and have been implicated in various processes including cell adhesion, tissue integration and remodelling, platelet activation, complement activation, pathogen recognition, endocytosis, and phagocytosis. CD302 was identified as a genetic fusion partner of human CD205 (DEC-205) in Hodgkins lymphoma cell lines and is classified as a group XV C-type lectin. The receptor consists of a single extracellular CTLD, a short spacer followed by a transmembrane region, and a cytoplasmic tail containing a putative
By Dr. Mercola While whole foods are healthy, there are certain caveats to consider even here. Lectins (not to be confused with the phospholipid lecithin) are carbohydrate-binding proteins that are widespread in the plant kingdom. An estimated 30 percent of fresh foods contain lectins.1 Even dairy contains lectins. Grass fed butter is an exception. Grass…
Sie sind hier: Glycopolymer Brushes for Specific Lectin Binding by Controlled Multivalent Presentation of N-acetyllactosamine Glycan Oligomers. ...
4R Health Products created by Dr Robert Siegel MD based on his 30 years of Aloe Vera research.He discovered special molecules in aloe vera gel that provide great benefits for supporting the immune system, to maintain and achieve optimal health.
Referred to: Immunology Department Camelia Botnar Laboratories Level 4 Great Ormond Street Hospital London WC1N 3JH General information Specimen transport: At room temperature Repeat frequency: At significant change of clinical...
Wheat germ agglutinin (WGA) is a plant protein that binds specifically to sugars expressed, among many others, by human gastrointestinal epithelialand immune cells. WGA is a toxic compound and an anti-nutritional factor, but recent works have shown that it may have potential as an anti-tumor drug and as a carrier for oral drugs. To quantitate the toxicity threshold for WGA on normal epithelial cells we previously investigated the effects of the lectin on differentiated Caco2 cells, and showed that in the micromolar range of concentrations WGA could alter the integrity of the epithelium layer and increase its permeability to both mannitol and dextran. WGA was shown to be uptaken by Caco2 cells and only approximately 0.1% molecules were observed to cross the epithelium layer by transcytosis. Here we show that at nanomolar concentrations WGA is unexpectedly bioactive onimmune cells. The supernatants of WGA-stimulated peripheral blood mononuclear cells (PBMC) can alter the integrity of the ...
Nine types of collectins have been defined: MBL = mannan-binding lectin (mannose-binding lectin) SP-A = surfactant protein A SP ... which participate in activation of the lectin pathway. MASP-2 has a cleavage activity and it is essential for forming lectin C3 ... Collectins (collagen-containing C-type lectins) are a part of the innate immune system. They form a family of collagenous Ca2+- ... Collectin MBL is involved in activation of the lectin complement pathway. There are three serine proteases, MASP-1, 2 and 3 ( ...
... is a plant-based lectin, but not a legume lectin, found in jackfruit. It has been studied for capturing O-glycoproteins ... Jacalin belongs to a family of galactose-binding lectins containing the Jacalin-like lectin domain and it has a tetrameric two- ... The lectin is blood group non-specific after neuraminidase treatment and agglutinates human erythrocytes at a concentration of ... The relative affinities of the lectin for galactose derivatives, as well as the structural basis of its T-antigen specificity, ...
The name lectin is a bit misleading because the family includes proteins with at least one C-type lectin domain (CTLD) which is ... DC‑associated C‑type lectin 1 (Dectin1) subfamily includes dectin 1/CLEC7A, DNGR1/CLEC9A, Myeloid C‑type lectin‑like receptor ( ... associating lectin (MDL)‑1 (CLEC5A), DC‑associated C‑type lectin 1 (Dectin1) subfamily, and DC immunoreceptor (DCIR) subfamily ... One very important collectin is mannan-binding lectin (MBL), a major PRR of the innate immune system that binds to a wide range ...
Mannan-binding lectins. Other assumptions may help to explain this resistance to FCoV infections by kittens. In the first weeks ... ACE and DC-SIGN are two trans-membrane retrovirus receptors (mannose receptors) which can bind "the plant lectins C-type ... Extensive data also shows that processes using sialic acid are directly involved in the interaction with the receptor's lectins ... Lozach, Pierre-Yves; Burleigh, Laura; Staropoli, Isabelle; Amara, Ali (2007). "The C Type Lectins DC-SIGN and L-SIGN". ...
Varki A, Cummings RD, Esko JD, Freeze HH, Stanley P, Bertozzi CR, Hart GW, Etzler M (2009). "P-type Lectins". Essentials of ... Imperial College Lectins Research Information UniProtKB/ Swiss-Prot entry for the human cation-independent mannose 6-phosphate ...
... can "hide" mannose antigens on the surface of host cells or bacteria from mannose-binding lectin.[citation needed] ... This finding is moreover supported by recent lectin staining studies and a molecular level survey on prokaryotic nonulosonic ... Mandal, C. (1990). "Sialic acid binding lectins". Experientia. 46 (5): 433-441. doi:10.1007/BF01954221. PMID 2189746. S2CID ...
During the 1940s, Boyd and Karl O. Renkonen independently discovered that lectins react differently to various blood types, ... Espino-Solis, Gerardo Pavel (April 2015). "Lectins: A brief review". Vitae. 22 (1): 9-11. doi:10.17533/udea.vitae.v22n1a01. ...
It does this with lectin-a protein made for binding proteins-named regenectin, which shares a family with other ... Kubo T, Arai T (September 1996). "Insect Lectins and Epimorphosis". Trends in Glycoscience and Glycotechnology. 8 (43): 357-364 ... Kubo T, Arai T (September 1996). "Insect Lectins and Epimorphosis". Trends in Glycoscience and Glycotechnology. 8 (43): 357-364 ...
V. Horoejsí, O. Chaloupecká, and j. Kocourek, Studies on lectins: XLIII. Isolation and characterization of the lectin from ...
ISBN 978-0-12-385968-6. Csaba, György (September 2016). "Lectins and Tetrahymena - A review". Acta Microbiologica et ...
While some companies were considering growing GM crops expressing lectin, GNA was an unlikely candidate. Lectin is toxic, ... Vasconcelos IM, Oliveira JT (September 2004). "Antinutritional properties of plant lectins". Toxicon. 44 (4): 385-403. doi: ... "Effect of diets containing genetically modified potatoes expressing Galanthus nivalis lectin on rat small intestine". Lancet. ... allowing the tuber to synthesise the GNA lectin protein. ...
Another study suggests that C-type lectins part of the mannose receptor (MR) family play a role as an alternative SRLV receptor ... The mannose receptor is a 180-kDa transmembrane protein with eight tandem C-type lectin carbohydrate recognition domains (CRD) ... Kerrigan AM, Brown GD (2009). "C-type lectins and phagocytosis". Immunobiology. 214 (7): 562-575. doi:10.1016/j.imbio.2008.11. ...
Cytotoxic lectins including modeccin act in a similar manner as ricin, a well understood toxic lectin, though each one has a ... Modeccin is a toxic lectin, a group of glycoproteins capable of binding specifically to sugar moieties. Different toxic lectins ... Cytotoxic lectins include ricin, abrin, modeccin, volkensin (least toxic, 10 and 40 times less cytotoxic than ricin and ... Olsnes S, Pihl A (1982). "Toxic lectins and related proteins". In Cohen P, Van Heyningen S (eds.). Molecular action of toxins ...
Hartley MR, Lord JM (September 2004). "Cytotoxic ribosome-inactivating lectins from plants". Biochimica et Biophysica Acta (BBA ...
C-type lectin-like receptors (CLRs) contain one or more C-type lectin (Ca2+ dependent carbohydrate-binding lectin) domains. ... C-Type Lectins in Immune Homeostasis. Current Topics in Microbiology and Immunology. 429: 103-115. doi:10.1007/82_2020_203. ... distinguished by extracellular regions containing immunoglobulin or C-type lectin domains. Nomenclature within these families ... region may contain multiple repeating protein domains and may be members of either the immunoglobulin or C-type lectin families ...
In general, lectins are used in blood typing and serology, and they are widely used in affinity chromatography for purifying ... Clavaria zollingeri contains lectins, a class of proteins that bind specific carbohydrates on the surface of cells, causing ... ISBN 978-81-85041-73-5. Jeune-Chung KH, Kim MK, Chung SR (1987). "Studies on lectins from mushrooms II. Screening of bioactive ... substance lectins from Korean wild mushrooms". Yakhak Hoeji (in Korean). 31 (4): 213-8. "Clavaria zollingeri Lév". NERI - The ...
Jeune-Chung KH, Kim MK, Chung SR (1987). "Studies on lectins from mushrooms II. Screening of bioactive substance lectins from ...
Sialic acid-binding Ig-like lectin 6 is a protein that in humans is encoded by the SIGLEC6 gene. The gene was originally named ... Sialic acid-binding immunoglobulin superfamily lectins". Journal of Biological Chemistry. 275 (12): 8625-32. doi:10.1074/jbc. ...
"Ask the Expert: Clearing Up Lectin Misconceptions". Today's Dietitian Magazine. 2017. Retrieved August 14, 2018. The lectin- ... The Plant Paradox Quick and Easy: The 30-Day Plan to Lose Weight, Feel Great, and Live Lectin-Free. New York: Harper Wave. 2019 ... He is best known for his disputed claims that lectins, a type of plant protein found in numerous foods, cause inflammation ... In 2022, he authored Unlocking the Keto Code which promotes a lectin-free ketogenic diet consisting of goat and sheep dairy ...
She characterized several lectins, including Vicia graminea lectin which is specific for human N blood group antigen, described ... Duk, M.; Lisowska, E.; Wu, J. H.; Wu, A. M. (1994-09-01). "The Biotin/Avidin-Mediated Microtiter Plate Lectin Assay with the ... Wu, Albert M.; Lisowska, Elwira; Duk, Maria; Yang, Zhangung (2008-03-27). "Lectins as tools in glycoconjugate research". ... Wu, Albert; Lisowska, Elwira; Duk, Maria; Zhangung, Yang (2009). "Lectins as tools in glycoconjugate research". Glycocnjugate ...
These foods contain the highly toxic lectin phytohemagglutinin, making as few as four raw beans toxic. This lectin is only ... Among the lectins known to have toxic effects is phytohaemagglutinin which occurs at relatively high levels in the seeds of ... Kotifani, Aislinn (2020-03-06). "The Truth About Lectins-Why Beans Have Gotten a Bad Rap - Blue Zones". Blue Zones. Retrieved ... Pressure cooking also deactivates the lectins. Cooking portal Food portal Beanpot Carry over cooking Combi steamer Haybox List ...
... structure World of Lectin, Gateway to lectins Proteopedia 1bxh con A in complex with methyl alpha1-2 ... and carbohydrate-binding specificity of lectins". In Liener, Irvin E.; Sharon, Nathan; Goldstein, Irwin J. (eds.). The Lectins ... Like most lectins, ConA is a homotetramer: each sub-unit (26.5kDa, 235 amino-acids, heavily glycated) binds a metallic atom ( ... ConA was the first lectin to be available on a commercial basis, and is widely used in biology and biochemistry to characterize ...
"Lectin from Peanuts". Sigma Aldrich. 2015. Retrieved 30 April 2015. "AABB". AABB. 2015. Retrieved 29 April 2015. Marcela ... Armed Forces Medical College, Pune Immunohaematology American Association of Blood Banks Lectin India portal Medicine portal ... The King of Lectins and Polyagglutination. In 1961, when Queen Elizabeth II visited India, Gregory Bird was in charge of ...
... (also BanLec-I or Banana lectin) is a lectin from the jacalin-related lectin family isolated from the fruit of the ... Crystal structures of BanLec suggest that the lectin has two saccharide binding sites. In 2010, BanLec was reported to be a ... Swanson MD, Winter HC, Goldstein IJ, Markovitz DM (March 2010). "A Lectin Isolated from Bananas Is a Potent Inhibitor of HIV ... The protein is highly stable, unfolding only at high temperatures All jacalin-related lectins feature type I beta-prism folding ...
Gupta GS (2012). Gupta GS (ed.). Animal Lectins: Form, Function and Clinical Applications. Wien: Springer-Verlag. doi:10.1007/ ... The asialoglycoprotein receptors (ASGPR) are lectins which bind asialoglycoprotein and glycoproteins from which a sialic acid ... and was the first mammalian lectin identified. The researchers transferred radioactively-labeled ceruloplasmin that had ... C-type lectins, All stub articles, Biochemistry stubs). ...
Dectin-2 is a member of the C-type lectin/C-type lectin-like domain (CTL/CTLD) superfamily. The encoded protein is a type II ... Dectin-2 is a member of C-type lectin receptor family, which is a group of pattern recognition receptors involved in antifungal ... Dectin-2 or C-type lectin domain containing 6A is a protein that in humans is encoded by the CLEC6A gene. ... Kerscher B, Willment JA, Brown GD (May 2013). "The Dectin-2 family of C-type lectin-like receptors: an update". International ...
Vautier S, Sousa M, Brown GD (December 2010). "C-type lectins, fungi and Th17 responses". Cytokine & Growth Factor Reviews. 21 ...
While some lectins such as cyanovirin and Urtica dioica agglutinin are thought likely to be too allergenic to be used ... Li Y, Zhang X, Chen G, Wei D, Chen F (2008). "Algal lectins for potential prevention of HIV transmission". Current Medicinal ... Huskens D, Schols D (July 2012). "Algal lectins as potential HIV microbicide candidates". Marine Drugs. 10 (7): 1476-97. doi: ... Ziółkowska NE, Wlodawer A (2006). "Structural studies of algal lectins with anti-HIV activity". Acta Biochimica Polonica. 53 (4 ...
Lectins from D. confragosa, tested against rabbit and human erythrocytes, were determined to have anti-H serological ... Pemberton R.T. (1994). "Agglutinins (lectins) from some British higher fungi". Mycological Research. 98 (3): 277-90. doi: ...
Lectin affinity chromatography is a form of affinity chromatography where lectins are used to separate components within the ... Lectins, such as concanavalin A are proteins which can bind specific alpha-D-mannose and alpha-D-glucose carbohydrate molecules ... Another example of a lectin is wheat germ agglutinin which binds D-N-acetyl-glucosamine. The most common application is to ... Although there are various ways to perform lectin affinity chromatography, the goal is extract a sugar ligand of the desired ...
Some lectins are poisonous, such as the ones found in kidney beans. This is why certain foods must be soaked to hydrolyse the ...
Mannose-binding lectin deficiency is a condition that affects the immune system. Explore symptoms, inheritance, genetics of ... Functional mannose-binding lectins are made up of two to six protein groups called trimers, which are each composed of three of ... Mannose-binding lectin plays an important role in the bodys immune response by attaching to foreign invaders such as bacteria ... Mannose-binding lectin deficiency is a condition that affects the immune system. People with this condition have low levels ( ...
Dear collegues, I am loking any informaton conserning lectin or lectin-contained medicaments which are available on the market ... Lectin medicaments. Dr.A.V.Timoshenko Timoshenko at Wed Jun 17 06:14:17 EST 1998 *Previous message: BIOSCI/ ...
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... Br J Nutr. 1993 Jul;70(1):313-21. ... As a result of their binding and endocytosis by the epithelial cells of the small intestine, all three lectins were growth ... as the presence of this lectin in the diet may harm higher animals at the concentrations required to be effective against most ...
C-type lectin superfamily member 10. DC-associated C-type lectin 2. dectin 2. dendritic cell-associated C-type lectin 2. ... C-type lectin domain family 6 member A. Names. C-type (calcium dependent, carbohydrate-recognition domain) lectin, superfamily ... C-type lectin domain containing 6Aprovided by HGNC. Primary source. HGNC:HGNC:14556 See related. Ensembl:ENSG00000205846 MIM: ... Deciphering the molecular basis of mycobacteria and lipoglycan recognition by the C-type lectin Dectin-2. Decout A, et al. Sci ...
Receptors, C-Type Lectin. Known as: C-Type Lectin Receptors, Lectin Receptors, C-Type, Receptors, C Type Lectin ...
What are lectins? Lectins are carbohydrate-binding proteins that are found in most foods. They can be harmful. Kidney beans are ... Lectins are not our enemies, and the foods Gundry prohibits are part of a science-based healthy diet. Avoiding them might lead ... In the book he describes many case reports of patients who improved on a lectin-limited diet, but he has done no studies with a ... Lectins play essential roles in human physiology. Among their many important functions, they regulate cell adhesion and are the ...
... rye and rice all contain chitin-binding lectins similar to wheat lectin (WGA). ... All three cereals contain lectin at the periphery of embryonic roots. Similar lectins were not detected in oats and pearl ... Rice, a species only distantty related to wheat, contains a lectin that is antigenically similar to the other cereal lectins ... All three cereals contain lectin at the periphery of embryonic roots. Similar lectins were not detected in oats and pearl ...
Immobilized lectins are prepared using affinity purified lectins. *Covalent attachment preserves lectin activity and minimizes ... Our agarose bound lectins are supplied at a constant concentration of lectin per ml of settled beads. The concentration for ... The technique we have developed to couple lectins to agarose beads inserts a hydrophilic spacer arm between the lectin and the ... The attachment of the lectins to the beads is carefully controlled to preserve lectin activity and minimize conformational ...
More info for Superfamily b.77.3: Mannose-binding lectins. Timeline for Superfamily b.77.3: Mannose-binding lectins: * ... Superfamily b.77.3: Mannose-binding lectins appears in SCOPe 2.06. *Superfamily b.77.3: Mannose-binding lectins appears in the ... b.77.3.1: Mannose-binding lectins [51102] (7 proteins). *. b.77.3.0: automated matches [191397] (1 protein). not a true family ... Lineage for Superfamily b.77.3: Mannose-binding lectins. *Root: SCOPe 2.07 *. Class b: All beta proteins [48724] (178 folds). ...
Lectins have been making a lot of headlines over the past year. What exactly are they, and do we need to avoid them? Medical ... What Are Lectins?. Lectins are a family of carbohydrate-binding proteins. Lectins are ubiquitous in the food supply, but they ... Should Lectins Be Avoided?. Garth Davis, MD, says people often ask him about this subject. "Like gluten and carbs, (lectins ... "If lectins are so poisonous, why would we see such amazing health in people whose diet is dominated by lectins?" ...
The lectin-imprinted hydrogel layer sensor chips showed larger SPR signal change in response to a target lectin than ... On the other hand, the SPR signal change of the lectin-imprinted hydrogel layer chip in the presence of other lectin was very ... The larger SPR signal change was attributed to the strong affinity constant of the lectin-imprinted hydrogel layer for the ... These results suggest that molecular recognition sites for the lectin were formed within the hydrogel layers by molecular ...
Do high lectin plants tend to have more lectins in their roots in general?. Thank you ... Do you happen to know? "The most potent lectin against the SARS-CoV-induced cytopathicity is the mannose-specific plant lectin ... and every other lectin they tested). I havent been able to find much info about this lectin, but assume it might be more ... When these lectins induce specific white blood cells to multiply, they are known as mitogens . Many plant lectins are mitogens ...
The Mac-2 antigen is a galactose-specific lectin that binds IgE. Cherayil, B.J., Weiner, S.J., Pillai, S. J. Exp. Med. (1989) [ ... Immunohistochemical and in situ hybridization analysis of galectin-3, a beta-galactoside binding lectin, in the urinary system ... Human breast carcinoma cDNA encoding a galactoside-binding lectin homologous to mouse Mac-2 antigen. Oda, Y., Leffler, H., ... Carbohydrate-binding protein 35 (Mac-2), a laminin-binding lectin, forms functional dimers using cysteine 186. Woo, H.J., Lotz ...
Lectin Labs is announcing the launch of its first commercial product based on its groundbreaking, ... Carson City - Lectin Labs is announcing the launch of its first commercial product based on its groundbreaking, proprietary ... NutraLec is based on a class of proteins called lectins which, the Company has found, have the ability to bind to harmful ... The Company has been awarded six U.S. patents for its lectin technologies, and many more U.S. and international cases are ...
... Larvie, Mykol. ... Mannose-binding lectin (MBL), a soluble factor of the innate immune system, is a pattern recognition molecule with a number of ... Mannose-binding lectin binds to amyloid \(\beta\) protein and modulates inflammation. Journal of Biomedicine and Biotechnology ... Mannose-Binding Lectin Binds to Amyloid \(\beta\) Protein and Modulates Inflammation. en_US. ...
The potential of the synthesized galactose analogues to act as competitive inhibitors in lectin-carbohydrate interactions is ... De novo synthesis and lectin binding studies of unsaturated carba-pyranoses† Timo Leermann,*a Oliver Block,b Michael A. L. ... novo synthesis and lectin binding studies of unsaturated carba. -pyranoses T. Leermann, O. Block, M. A. L. Podeschwa, U. ... in lectin-. carbohydrate. interactions is investigated by means of Surface Plasmon Resonance (SPR) Spectroscopy. . ...
BGR, Dendritic Cell-Associated C-Type Lectin-1, Dendritic Cell-Associated C-Type Lectin 1, C-Type Lectin Domain Family 7, ... Member A, Lectin-Like Receptor 1, CD369 Antigen, CANDF4, SCARE2, CD369, C-Type Lectin Domain Containing 7A, C-Type Lectin ... C-Type Lectin Superfamily Member 12, DC-Associated C-Type Lectin 1, Beta-Glucan Receptor, Dectin-1, CLECSF12, DECTIN1. ... C-type lectin domain family 7 member A 1 or CLEC7A is a protein, that in the innate immune system, acts against fungal ...
A range of products available for the purification of lectins and galactose binding molecules ... Examples of N-acetyl-D- glucosamine specific lectins are wheat germ agglutinin, elderberry lectin, Maackia amurensis lectin, ... Lectin Purification. G-Biosciences offer a range of products for the rapid purification of lectins, galactosidases and other ... Jacalin (Artocarpus integrifolia lectin). G-Biosciences Jacalin (Artocarpus integrifolia lectin) is isolated from the seeds of ...
Lectin Blot-Based Profiling of Salivary Fluid Glycoproteins Distinguishes Different Patterns Among Individuals. Presenting ...
An international team of scientists have engineered protein banana lectin, or BanLec, to create a new version that can fight ... Posted in News , Tagged Antiviral, BanLec, Genetic engineering, Lectin, Proteins, virus An international team of scientists ... "But its also exciting to have created it by engineering a lectin molecule for the first time, by understanding and then ... have engineered protein banana lectin, or BanLec, to create a new version that can fight off a range of viruses without ...
... while lectin is a plant protein that has the ability to bin ... The key difference between leptin and lectin is that leptin is ... 3. What is Lectin. 4. Similarities Between Leptin and Lectin. 5. Side by Side Comparison - Leptin vs Lectin in Tabular Form. 6 ... What is Lectin?. Lectin is a protein which is of plant origin. They are found in high concentrations in wheat, rye, barley, ... The key difference between leptin and lectin is that leptin is a hormone secreted by fat cells, while lectin is a plant protein ...
Rabbit C Type Lectin Domain Family 3 Member B CLEC3B ELISA Kit - - Product info ... CLEC10A (C-type Lectin Domain Family 10 Member A, C-type Lectin Superfamily Member 14, Macrophage Lectin 2, CD301, CLECSF13, ... CD302, ID (CD302 Antigen, C-type Lectin Domain Family 13 Member A, C-type Lectin BIMLEC, Type I Transmembrane C-type Lectin ... CD302, ID (CD302 Antigen, C-type Lectin Domain Family 13 Member A, C-type Lectin BIMLEC, Type I Transmembrane C-type Lectin ...
The seed lectin from elderberry sambucus nigra l and its relationship to the two bark lectins. Meeting of The Belgian ... Isolation and characterization of a seed lectin from elderberry (Sambucus nigra L.) and its relationship to the bark lectins. ... The Neu Ac(-2,6)-Gal/Gal Nac-Binding Lectin from Elderberry ( Sambucus Nigra ) Bark, a type-2 Ribosome-Inactivating Protein ... The NeuAc(alpha-2,6)-Gal/GalNAc-binding lectin from elderberry (Sambucus nigra) bark, a type-2 ribosome-inactivating protein ...
Maackia Amurensis Lectin I (MAL I), Biotinylated (B-1315-2) by Vector Laboratories ... Lectins & Glycobiology Reagents [Lectins ], Galactose (Beta1;4) N-acetylglucosamine binding lectin. Application:. ... This lectin is the leucoagglutinin or mitogenic isolectin from Maackia seeds. Maackia amurensis lectin I binds gal (β-1,4) ... First the biotinylated lectin is added, followed by the VECTASTAIN ABC Reagent, Avidin D conjugate, or streptavidin derivative ...
Home / Lectins By Latin Name / Glycine max Lectin. Glycine max Gel -SBA- Immobilized Lectin, 2mL. ... SKU: A-1301-2 Categories: Agarose Beads, Agarose Beads, Glycine max Lectin Tags: Soybean, A-1301-2, lectin, SBA, Affinity- ... Glycine max Gel -SBA- Immobilized Lectin, 2mL With the exception of Diagnostic Microbiology Products all biochemicals described ...
Understanding the role of L-type lectin receptor kinases in Phytophthora resistance: Abstract Phytophthora pathogens are ... Understanding the role of L-type lectin receptor kinases in Phytophthora resistance. Authors * Wang, Y. ... One RLK that was previously identified as a novel Phytophthora resistance component is the Arabidopsis L-type lectin receptor ... Cell death induction however, only required the kinase, not the lectin domain. Since transient expression of both LecRKs in ...
Synthesis of 3-amido-3-deoxy-beta-D-talopyranosides: all-cis-substituted pyranosides as lectin inhibitors. In: Tetrahedron. ... Synthesis of 3-amido-3-deoxy-beta-D-talopyranosides: all-cis-substituted pyranosides as lectin inhibitors. / Öberg, Christopher ... Synthesis of 3-amido-3-deoxy-beta-D-talopyranosides: all-cis-substituted pyranosides as lectin inhibitors. Tetrahedron. 2011;67 ... title = "Synthesis of 3-amido-3-deoxy-beta-D-talopyranosides: all-cis-substituted pyranosides as lectin inhibitors", ...
  • Lectins are a family of carbohydrate-binding proteins. (
  • NutraLec is based on a class of proteins called lectins which, the Company has found, have the ability to bind to harmful bacteria and make them incapable of causing disease. (
  • Purification of N-acetyl-D-galactosamine/ D-galactose specific lectins and carbohydrate binding proteins/enzymes is consistent and affordable with N-Acetyl-D-Galactosamine Agarose . (
  • N-Acetyl-D- Glucosamine Agarose is used in purification of N-acetyl-D- glucosamine specific lectins and carbohydrate binding proteins/enzymes. (
  • Lectins are proteins or glycoproteins that have at least one noncatalytic domain that binds specifically and reversibly to monosaccharides or oligosaccharides. (
  • Both leptin and lectin are proteins. (
  • Lectins are carbohydrate recognizing proteins that bind mono- and oligosaccharide specifically and reversibly but are devoid of catalytic activity. (
  • Lectins are carbohydrate-binding proteins with remarkable ability to recognize and bind reversibly to carbohydrate moieties of complex glycoconjugates without altering the covalent structure of the bound glycosyl ligands[9]. (
  • Bacterial colonization of human tissues is mediated by interaction of bacterial surface proteins - lectins - with cellular surface carbohydrates. (
  • Some ANTIBODIES and carbohydrate-metabolizing proteins ( ENZYMES ) also bind to carbohydrates, however they are not considered lectins. (
  • PLANT LECTINS are carbohydrate-binding proteins that have been primarily identified by their hemagglutinating activity ( HEMAGGLUTININS ). (
  • Leptin is a hormone secreted from fat cells that helps to regulate body weight while lectin is a protein of plant origin that binds with carbohydrates. (
  • In contrast, lectin binds with carbohydrates and reduces the body's ability to absorb nutrients. (
  • Maackia amurensis lectin I binds gal (β-1,4) glcNAc but tolerates substitution of N -acetyllactosamine with sialic acid at the 3 position of galactose. (
  • This lectin preferably binds to [Galβ(1-4)GlcNAc, poly LacNAc] glycan epitopes. (
  • Vicia villosa lectin, which binds selectively to T145 of activated T cells and which is cytotoxic for cytolytic T cell lines, has been used to select lectin-resistant mutants from these lines. (
  • Included in this family is a lectin found in black locust (Robinia pseudoacacia) bark, which binds chitin but lacks chitinase activity. (
  • The root also possesses what I consider one of the most interesting plant lectins, known as Urtica Dioica Agglutinin (UDA). (
  • Examples of N-acetyl-D-galactosamine specific lectins are lectin from Glycine max, Dolichos biflorus, Ricinus communis agglutinin, Peanut agglutinin, Jacalin etc. (
  • Examples of N-acetyl-D- glucosamine specific lectins are wheat germ agglutinin, elderberry lectin, Maackia amurensis lectin, etc. (
  • Both P. marinus strains bound with lower intensity to Maclura pomifera agglutinin, Bauhinia purpurea agglutinin, soybean agglutinin ( N -acetyl- d -galactosamine-specific lectins), peanut agglutinin (PNA) (terminal galactose specific), and Griffonia simplicifolia II (GlcNAc specific). (
  • The green pea lectin (pisum sativum agglutinin) is "much less toxic" than others, according to renowned legume opponent Dr. Loren Cordain's research . (
  • The 45-kDa form was pulled down by lectin concanavalin A (Con A) and Galant husnivalis lectin (GNL), indicating an immature glycan with a high amount of mannose (Man), whereas the 65-kDa form is a mature glycan composed of acetylglucosamine (GlcNAc) and poly-N-acetyllactosame (poly-LacNAc) that was pulled down by wheat germ agglutinin (WGA) and tomato lectin, respectively. (
  • People with this condition have low levels (deficiency) of an immune system protein called mannose-binding lectin in their blood. (
  • This gene provides instructions for making a protein that assembles into a complex called mannose-binding lectin. (
  • Functional mannose-binding lectins are made up of two to six protein groups called trimers, which are each composed of three of the protein pieces (subunits) produced from the MBL2 gene. (
  • A decrease in the availability of the normal subunit protein may lead to a reduction of the functional mannose-binding lectin in blood. (
  • However, not everyone with a change in the MBL2 gene has decreased levels of mannose-binding lectin, and not everyone with decreased protein levels is prone to infection. (
  • The protein encoded by this gene is a type II membrane receptor with an extracellular C-type lectin-like domain fold. (
  • Last year, Steven Gundry, MD, released The Plant Paradox: The Hidden Dangers in Healthy Foods That Cause Disease and Weight Gain , which claimed that lectins, a type of protein in many plant foods, are the culprits behind autoimmune diseases, coronary artery disease, type 2 diabetes, and a host of other health problems. (
  • the authors noted that lectins such as UDA do not affect binding of the S-protein to the ACE2 receptor, but rather seem to inhibit viral entry at some undefined later stage. (
  • C-type lectin domain family 7 member A 1 or CLEC7A is a protein, that in the innate immune system, acts against fungal pathogens. (
  • An international team of scientists have engineered protein banana lectin, or BanLec, to create a new version that can fight off a range of viruses without unwanted side effects. (
  • On the other hand, lectin is a protein that is of plant origin. (
  • Lectin is a protein which is of plant origin. (
  • In contrast, lectin is a plant protein. (
  • Here, using state-of-the-art structure prediction methods and structural modeling, we show that A33 has most likely evolved from a C-type lectin-like protein. (
  • Lectins were discovered in the last century when Stillmark (1889) presented evidence that the extreme toxicity of castor beans (Ricinus communis) could be attributed to a protein fraction which agglutinated red blood cella and which he called «ricin. (
  • The rLSL-N is an N-terminal domain of carbohydrate-binding protein (Lectin) derived from Laetiporus sulphureus . (
  • Lectin like protein whose expression is induced upon treatment with chitin oligomers. (
  • The CX5 antibody is specific for mouse NKG2D (CD314, KLRK1), a lectin-like type II transmembrane receptor protein. (
  • The potential of the synthesized galactose analogues to act as competitive inhibitors in lectin- carbohydrate interactions is investigated by means of Surface Plasmon Resonance (SPR) Spectroscopy . (
  • G-Biosciences offer a range of products for the rapid purification of lectins, galactosidases and other galactose-binding molecules. (
  • G-Biosciences Immobilized D-Galactose is for the purification of lectins, galactosidases and other galactose-, N-acetylgalactosamine- or carbohydrate binding molecules. (
  • Jacalin is a α-D-galactose binding lectin consisting of a tetrameric two chain structure with a molecular weight of ~66kDa. (
  • However, MAL I does not appear to bind this structure when substitution with sialic acid is on the 6 position of galactose.Biotinylated Maackia amurensis lectin has an appropriate number of biotins bound to provide the optimum staining characteristics for this lectin. (
  • The characterization of lectins as assailants on our health is "utter nonsense," Davis says. (
  • This study explores the isolation and partial characterization of lectin from leaves of Vernonia amygdalina , Talinum triangulare, Telfairia occidentalis and the kernels of Irvingia gabonensis focusing on their degree of antibacterial activities against some selected gastrointestinal pathogenic bacteria. (
  • Understanding the role of L-type lectin receptor kinases in Ph. (
  • One RLK that was previously identified as a novel Phytophthora resistance component is the Arabidopsis L-type lectin receptor kinase LecRK-I.9. (
  • N-glycosylation of mannose receptor (CD206) regulates glycan binding by C-type lectin domains. (
  • The macrophage mannose receptor (MR, CD206) is a transmembrane endocytic lectin receptor, expressed in selected immune and endothelial cells , and is involved in immunity and maintaining homeostasis . (
  • Identification of a site on mannan-binding lectin critical for enhancement of phagocytosis. (
  • We hypothesised that mannan-binding lectin (MBL) deficiency may confer risk of obesity and insulin resistance. (
  • Ehrlich (1891) compared it with the toxic abrin from the jeriquinty seeds (Abrus precatorus) and thus initiated his immunological research on the specificity of antisera obtained by the use of these lectins. (
  • Lectin-binding specificity was confirmed by sugar inhibition and Kolmogorov-Smirnov statistics. (
  • Adam J, et al: Engineering of PA-IIL lectin from Pseudomonas aeruginosa - Unravelling the role of the specificity loop for sugar preference. (
  • Recent progress in glycobiology has emphasized that numerous pathogens ranging from viruses and bacteria to pluricellular parasites use lectins as tools to recognize and bind to the oligosaccharides exposed by target cells and tissues[1]. (
  • PA-IIL is Pseudomonas aeruginosa tetrameric lectin, which contains two calcium ions in each binding site and recognizes fucosylated oligosaccharides [ 1 ]. (
  • Relatively common mutations in the MBL2 gene can lead to mannose-binding lectin deficiency. (
  • Mutations in the MBL2 gene can reduce the production of the mannose-binding lectin subunit or eliminate the subunit's ability to assemble into functional mannose-binding lectin. (
  • Mannose-binding lectin concentrations, MBL2 polymorphisms, and susceptibility to respiratory tract infections in young men. (
  • Mannose-binding lectin 2 (MBL2) gene polymorphism in asthma and atopy among adults. (
  • This study reports on the identification of Perkinsus marinus surface carbohydrates by flow cytometric analyses of fluorescein isothiocyanate-conjugated lectin binding. (
  • Mannose-binding lectin deficiency is a condition that affects the immune system. (
  • People with mannose-binding lectin deficiency can develop infections of the upper respiratory tract and other body systems. (
  • Infants and young children with mannose-binding lectin deficiency seem to be more susceptible to infections than affected adults, but adults can also develop recurrent infections. (
  • Researchers believe that a number of factors, including other genetic and environmental factors, are involved in the development of mannose-binding lectin deficiency and susceptibility to infection. (
  • The inheritance pattern of mannose-binding lectin deficiency is unclear. (
  • It is important to note that people inherit an increased risk of developing mannose-binding lectin deficiency, not the condition itself. (
  • Mannose-binding lectin (MBL), a soluble factor of the innate immune system, is a pattern recognition molecule with a number of known ligands, including viruses, bacteria, and molecules from abnormal self tissues. (
  • however, many affected individuals have no signs or symptoms related to low mannose-binding lectin levels. (
  • Mannose-binding lectin plays an important role in the body's immune response by attaching to foreign invaders such as bacteria, viruses, or yeast and turning on (activating) the complement system . (
  • Mannose-binding lectin can also stimulate special immune cells to engulf and break down the attached pathogen. (
  • With decreased levels of mannose-binding lectin, the body does not recognize and fight foreign invaders efficiently. (
  • Effects of GNA and other mannose binding lectins on development and fecundity of the peach-potato aphid Myzus persicae. (
  • In contrast, the collectin mannose binding lectin C (MBL-C) but not MBL-A led to efficient lectin pathway activation on A. fumigatus in the absence of ficolin-A. In addition, ficolin-A opsonization led to a modulation of the proinflammatory cytokine interleukin-8. (
  • Eight of the ten extracellular domains of the MR are C-type lectin domains (CTLDs) which mediate the binding of mannose , fucose , and GlcNAc in a calcium -dependent manner. (
  • Is Plasma Level of Mannose Binding Lectin Associated With Reproductive Failure? (
  • Mannose-binding lectin 2 gene polymorphisms and predisposition to allergic bronchial asthma in a western Romanian children population: an observational study. (
  • Mannose-binding lectin gene polymorphisms in infants with bronchiolitis and post-bronchiolitis wheezing. (
  • Mannose-binding lectin genotype and serum levels in patients with chronic and allergic pulmonary aspergillosis. (
  • Mannose binding lectin gene polymorphisms and asthma. (
  • The role of mannose-binding lectin in pneumococcal infection. (
  • Lectins are ubiquitous in the food supply, but they occur in especially high numbers in whole grains, legumes, potatoes, and fruit (including fruits that we typically consider vegetables, such as tomatoes and bell peppers)-in other words, a whole lot of the things that whole food, plant-based eaters rely on and enjoy every day. (
  • Plant lectins like UDA probably target viral attachment and fusion, and its eventual exit (exocytosis or egress) from the cell. (
  • A new plant lectin from elderberry (Sambucus nigra L.) bark, which was shown by immunochemical techniques to bind specifically to terminal Neu5Ac(alpha 2-6)Gal/GalNAc residues of glycoconjugates, was immobilized onto Sepharose 4B (SNA-Sepharose) and its carbohydrate binding properties was determined using a series of standard compounds. (
  • 60. Lectin-Free/Plant Paradox Discussion Pt. (
  • Lectins have been reported in more than 800 plant species (Lis and Sharon, 1973). (
  • Agglutination of red blood cells by lectin from Talinum triangulare were significantly enhanced by D-lactose, D-maltose and D-glucose compared to lectin from other plant materials. (
  • The study showed that the lectins from the different plant materials were capable of inhibiting the growth of the bacterial isolates. (
  • Although some plant lectins are resistant to heat, heat processing can reduce their toxicity identified by consumption of food with high content of lectins while low temperature or insufficient cooking may be insufficient to completely eliminate them. (
  • While leguminous seeds are particularly rich sources of lectin, lectins have also been isolated from other plant families and adopted for alternative therapy[13-14]. (
  • Lectin activities in roots, nodules, stems and leaves of 1-6 week old peanut plant (A. hypogaea) were checked by erythrocyte (human and rabbit) agglutination and sugar inhibition assays. (
  • In plants, legume lectins are the best know lectin family[12]. (
  • Recommended product for eluting glycoconjugates bound to this agarose-lectin: Glycoprotein Eluting solution, Cat. (
  • I purchased an agarose bound lectin from Vector Labs. (
  • Our agarose lectin products are supplied as hydrated matrix solutions in amber glass bottles. (
  • 1) Draw (pipet) the desired amount of settled agarose-lectin (gel) from the stock bottle into the prepared column and let the buffer drain by gravity. (
  • What are recommended conditions for using the agarose-lectin in chromatography? (
  • G-Biosciences' Jacalin (Artocarpus integrifolia lectin) is isolated from the seeds of jackfruit and purified by affinity chromatography. (
  • These compounds, known as "lectins" (from the Latin verb "legere," to choose), are extracted by the Company exclusively from botanical sources, many of which are present in our diet, and are being developed as nutraceutical and pharmaceutical microbicides which inhibit or destroy the infectivity of pathogenic viruses and bacteria (microbes) by binding to their carbohydrate surfaces. (
  • MBL binding to other known carbohydrate ligands is calcium-dependent and has been attributed to the carbohydrate-recognition domain, a common feature of other C-type lectins. (
  • The carbohydrate serves as a targeting agent and newly identified fragments increase its interaction with the lectin. (
  • However, a variety of lectins occur in animal species where they serve diverse array of functions through specific carbohydrate recognition. (
  • Although the transfer of the gene of WGA into crop plants has been advocated to increase their insect resistance, as the presence of this lectin in the diet may harm higher animals at the concentrations required to be effective against most pests, its use in plants as natural insecticide is not without health risks for man. (
  • Recombinant TC14-3 could reproduce all the activities of native lectins by itself, which was accelerated by recombinant TC14-2. (
  • A mutation in the kinase domain abolished the ability of LecRK-IX.1 and LecRK-IX.2 to induce Phytophthora resistance as did deletion of the lectin domain. (
  • Cell death induction however, only required the kinase, not the lectin domain. (
  • Saturation of bacterial surface lectins by specially designed compounds might prevent adhesion to host tissues and thus suppress the infection. (
  • All of them are cytolytically active, bind up to 100-fold less lectin than the parental lines, but still express T145 or a closely related glycoprotein. (
  • Lentil-lectin-purified glycoprotein antigens derived from VZV-infected human fibroblast cells (obtained through CRADA with Merck & Co.) is coated on the wells of a 96-well microtiter plate, which is subsequently incubated with a diluted test specimen. (
  • IMSEAR at SEARO: Vegetative tissue lectins of peanut (A. hypogaea). (
  • This lectin is the leucoagglutinin or mitogenic isolectin from Maackia seeds. (
  • This conjugate is supplied essentially free of unconjugated biotins and is preserved with sodium sodium azide.This biotinylated lectin is an ideal intermediate for examining glycoconjugates using the Biotin-Avidin/Streptavidin System. (
  • First the biotinylated lectin is added, followed by the VECTASTAIN ABC Reagent, Avidin D conjugate, or streptavidin derivative. (
  • Cystic kidney cells in vitro stained positive for lectins from Concanavalia ensiformis (concanavalin A), Triticum vulgaris, Erythrina cristagalli, Ulex europeaus, and Arachis hypogaea. (
  • He says lectins cause inflammation, stimulate weight gain, and cause leaky gut syndrome. (
  • While it's true that in patients with celiac disease or gluten sensitivity, gluten (which is a type of lectin) causes inflammation that does serious long-term damage, for the vast majority of people, the fear of inflammation is overblown, Kahn says. (
  • As a result of their binding and endocytosis by the epithelial cells of the small intestine, all three lectins were growth factors for the gut and interfered with its metabolism and function to varying degrees. (
  • Consequently, rLSL-N exhibits a weak hemagglutination activity to a rabbit reticulocyte through the glycan binding to subsites of the lectin. (
  • Unfortunately, chickpeas contain toxins like lectins that can cause food poisoning and make you very sick if they are consumed raw, so it's important to cook them properly. (
  • Wang, H. X. and Ng, T. B. Examination of lectins, polysaccharopeptide, polysaccharide, alkaloid, coumarin and trypsin inhibitors for inhibitory activity against human immunodeficiency virus reverse transcriptase and glycohydrolases. (
  • Some lectins are poisonous, such as the ones found in kidney beans . (
  • If lectins are so poisonous, why would we see such amazing health in people whose diet is dominated by lectins? (
  • But it's also exciting to have created it by engineering a lectin molecule for the first time, by understanding and then targeting the structure. (
  • In investigations of the lectin pathway of complement activation, ficolin-A-opsonized conidia did not lead to lectin pathway-specific C4 deposition. (
  • However, when UDA was exposed to the amino sugar N-acetylglucosamine the UDA lectin did not inhibit the virus infection. (
  • Deciphering the molecular basis of mycobacteria and lipoglycan recognition by the C-type lectin Dectin-2. (
  • Molecularly imprinted hydrogel layers with lectin-recognition sites were prepared on surface plasmon resonance (SPR) sensor chips via surface-initiated atom transfer radical polymerization (SI-ATRP) combined with molecular imprinting. (
  • These results suggest that molecular recognition sites for the lectin were formed within the hydrogel layers by molecular imprinting. (
  • This paper describes that SI-ATRP with biomolecular imprinting is a useful method to design highly sensitive and selective SPR sensor chips with molecular recognition sites for a target lectin. (
  • This complex of lectins, when applied in vitro to tunicate multipotent cells of epithelial origin, blocked cell proliferation and induced cell aggregation. (
  • Rice, a species only distantty related to wheat, contains a lectin that is antigenically similar to the other cereal lectins and located at the periphery of embryonic roots and throughut the coleoptile. (
  • Identification of Cell Surface Markers to Differentiate Rat Endothelial and Fibroblast Cells Using Lectin Arrays and LC-ESI-MS/MS . Analytical Chemistry. (
  • Antigenically similar chitin-binding lectins are present in the embryos of wheat, barley, and rye, members of the Triticeae tribe of the grass family (Gramineae). (
  • It is a member of the C-type lectin family, expressed as a disulfide-linked homodimer. (
  • This conserved domain family includes a large number of catalytically inactive chitinase-like lectins (chitolectins) including YKL-39, YKL-40 (HCGP39), YM1, oviductin, and AMCase (acidic mammalian chitinase), as well as catalytically active chitotriosidases. (
  • Specific applications include the purification of galactosidases, C-type lectins, enterotoxins and cholera toxin. (
  • Barley, rye and rice all contain chitin-binding lectins similar to wheat lectin (WGA). (
  • Lectin is absent from the coleoptile of barley but is present in the outer surface cells of this organ in wheat and in both inner and outer surface cells of rye coleoptiles. (