AnatomyChemicals and DrugsAnalytical, Diagnostic and Therapeutic Techniques and EquipmentPhenomena and ProcessesDisciplines and OccupationsInformation Science
LamininReceptors, LamininBasement MembraneFibronectinsCell AdhesionIntegrin alpha6beta1IntegrinsExtracellular MatrixDystroglycansCollagenIntegrin alpha6Integrin alpha3beta1Lutheran Blood-Group SystemExtracellular Matrix ProteinsAntigens, CD29Collagen Type IVIntegrin alpha6beta4Molecular Sequence DataCells, CulturedCell Adhesion MoleculesAmino Acid SequenceFluorescent Antibody TechniqueCell MovementImmunohistochemistryIntegrin alpha3Receptors, ImmunologicProtein BindingPeptide FragmentsCell LineMicroscopy, ElectronNeuritesHeparan Sulfate ProteoglycansTumor Cells, CulturedReceptors, CollagenMembrane GlycoproteinsGlycoproteinsHeparinEpithelium
Laminin
Large, noncollagenous glycoprotein with antigenic properties. It is localized in the basement membrane lamina lucida and functions to bind epithelial cells to the basement membrane. Evidence suggests that the protein plays a role in tumor invasion.
Receptors, Laminin
Glycoprotein molecules on the surface of cells that react with or bind to laminin whose function allows the binding of epithelial cells to the basement membrane. The molecular weight of this high-affinity receptor is 67 kD.
Basement Membrane
A darkly stained mat-like EXTRACELLULAR MATRIX (ECM) that separates cell layers, such as EPITHELIUM from ENDOTHELIUM or a layer of CONNECTIVE TISSUE. The ECM layer that supports an overlying EPITHELIUM or ENDOTHELIUM is called basal lamina. Basement membrane (BM) can be formed by the fusion of either two adjacent basal laminae or a basal lamina with an adjacent reticular lamina of connective tissue. BM, composed mainly of TYPE IV COLLAGEN; glycoprotein LAMININ; and PROTEOGLYCAN, provides barriers as well as channels between interacting cell layers.
Fibronectins
Glycoproteins found on the surfaces of cells, particularly in fibrillar structures. The proteins are lost or reduced when these cells undergo viral or chemical transformation. They are highly susceptible to proteolysis and are substrates for activated blood coagulation factor VIII. The forms present in plasma are called cold-insoluble globulins.
Cell Adhesion
Adherence of cells to surfaces or to other cells.
Integrin alpha6beta1
A cell surface receptor mediating cell adhesion to the EXTRACELLULAR MATRIX and to other cells via binding to LAMININ. It is involved in cell migration, embryonic development, leukocyte activation and tumor cell invasiveness. Integrin alpha6beta1 is the major laminin receptor on PLATELETS; LEUKOCYTES; and many EPITHELIAL CELLS, and ligand binding may activate a number of signal transduction pathways. Alternative splicing of the cytoplasmic domain of the alpha6 subunit (INTEGRIN ALPHA6) results in the formation of A and B isoforms of the heterodimer, which are expressed in a tissue-specific manner.
Integrins
A family of transmembrane glycoproteins (MEMBRANE GLYCOPROTEINS) consisting of noncovalent heterodimers. They interact with a wide variety of ligands including EXTRACELLULAR MATRIX PROTEINS; COMPLEMENT, and other cells, while their intracellular domains interact with the CYTOSKELETON. The integrins consist of at least three identified families: the cytoadhesin receptors(RECEPTORS, CYTOADHESIN), the leukocyte adhesion receptors (RECEPTORS, LEUKOCYTE ADHESION), and the VERY LATE ANTIGEN RECEPTORS. Each family contains a common beta-subunit (INTEGRIN BETA CHAINS) combined with one or more distinct alpha-subunits (INTEGRIN ALPHA CHAINS). These receptors participate in cell-matrix and cell-cell adhesion in many physiologically important processes, including embryological development; HEMOSTASIS; THROMBOSIS; WOUND HEALING; immune and nonimmune defense mechanisms; and oncogenic transformation.
Extracellular Matrix
A meshwork-like substance found within the extracellular space and in association with the basement membrane of the cell surface. It promotes cellular proliferation and provides a supporting structure to which cells or cell lysates in culture dishes adhere.
Dystroglycans
Dystrophin-associated proteins that play role in the formation of a transmembrane link between laminin-2 and DYSTROPHIN. Both the alpha and the beta subtypes of dystroglycan originate via POST-TRANSLATIONAL PROTEIN PROCESSING of a single precursor protein.
Collagen
A polypeptide substance comprising about one third of the total protein in mammalian organisms. It is the main constituent of SKIN; CONNECTIVE TISSUE; and the organic substance of bones (BONE AND BONES) and teeth (TOOTH).
Integrin alpha6
An integrin alpha subunit that primarily associates with INTEGRIN BETA1 or INTEGRIN BETA4 to form laminin-binding heterodimers. Integrin alpha6 has two alternatively spliced isoforms: integrin alpha6A and integrin alpha6B, which differ in their cytoplasmic domains and are regulated in a tissue-specific and developmental stage-specific manner.
Integrin alpha3beta1
Cell surface receptor for LAMININ, epiligrin, FIBRONECTINS, entactin, and COLLAGEN. Integrin alpha3beta1 is the major integrin present in EPITHELIAL CELLS, where it plays a role in the assembly of BASEMENT MEMBRANE as well as in cell migration, and may regulate the functions of other integrins. Two alternatively spliced isoforms of the alpha subunit (INTEGRIN ALPHA3), are differentially expressed in different cell types.
Lutheran Blood-Group System
A complex blood group system having pairs of alternate antigens and amorphic genes, but also subject to a dominant independently segregating repressor.
Extracellular Matrix Proteins
Macromolecular organic compounds that contain carbon, hydrogen, oxygen, nitrogen, and usually, sulfur. These macromolecules (proteins) form an intricate meshwork in which cells are embedded to construct tissues. Variations in the relative types of macromolecules and their organization determine the type of extracellular matrix, each adapted to the functional requirements of the tissue. The two main classes of macromolecules that form the extracellular matrix are: glycosaminoglycans, usually linked to proteins (proteoglycans), and fibrous proteins (e.g., COLLAGEN; ELASTIN; FIBRONECTINS; and LAMININ).
Antigens, CD29
Integrin beta-1 chains which are expressed as heterodimers that are noncovalently associated with specific alpha-chains of the CD49 family (CD49a-f). CD29 is expressed on resting and activated leukocytes and is a marker for all of the very late activation antigens on cells. (from: Barclay et al., The Leukocyte Antigen FactsBook, 1993, p164)
Collagen Type IV
A non-fibrillar collagen found in the structure of BASEMENT MEMBRANE. Collagen type IV molecules assemble to form a sheet-like network which is involved in maintaining the structural integrity of basement membranes. The predominant form of the protein is comprised of two alpha1(IV) subunits and one alpha2(IV) subunit, however, at least six different alpha subunits can be incorporated into the heterotrimer.
Integrin alpha6beta4
This intrgrin is a key component of HEMIDESMOSOMES and is required for their formation and maintenance in epithelial cells. Integrin alpha6beta4 is also found on thymocytes, fibroblasts, and Schwann cells, where it functions as a laminin receptor (RECEPTORS, LAMININ) and is involved in wound healing, cell migration, and tumor invasiveness.
Molecular Sequence Data
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
Cells, Cultured
Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others.
Cell Adhesion Molecules
Surface ligands, usually glycoproteins, that mediate cell-to-cell adhesion. Their functions include the assembly and interconnection of various vertebrate systems, as well as maintenance of tissue integration, wound healing, morphogenic movements, cellular migrations, and metastasis.
Amino Acid Sequence
The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.
Fluorescent Antibody Technique
Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
Cell Movement
The movement of cells from one location to another. Distinguish from CYTOKINESIS which is the process of dividing the CYTOPLASM of a cell.
Immunohistochemistry
Histochemical localization of immunoreactive substances using labeled antibodies as reagents.
Integrin alpha3
An integrin alpha subunit that occurs as alternatively spliced isoforms. The isoforms are differentially expressed in specific cell types and at specific developmental stages. Integrin alpha3 combines with INTEGRIN BETA1 to form INTEGRIN ALPHA3BETA1 which is a heterodimer found primarily in epithelial cells.
Receptors, Immunologic
Cell surface molecules on cells of the immune system that specifically bind surface molecules or messenger molecules and trigger changes in the behavior of cells. Although these receptors were first identified in the immune system, many have important functions elsewhere.
Protein Binding
The process in which substances, either endogenous or exogenous, bind to proteins, peptides, enzymes, protein precursors, or allied compounds. Specific protein-binding measures are often used as assays in diagnostic assessments.
Peptide Fragments
Partial proteins formed by partial hydrolysis of complete proteins or generated through PROTEIN ENGINEERING techniques.
Cell Line
Established cell cultures that have the potential to propagate indefinitely.
Microscopy, Electron
Microscopy using an electron beam, instead of light, to visualize the sample, thereby allowing much greater magnification. The interactions of ELECTRONS with specimens are used to provide information about the fine structure of that specimen. In TRANSMISSION ELECTRON MICROSCOPY the reactions of the electrons that are transmitted through the specimen are imaged. In SCANNING ELECTRON MICROSCOPY an electron beam falls at a non-normal angle on the specimen and the image is derived from the reactions occurring above the plane of the specimen.
Neurites
In tissue culture, hairlike projections of neurons stimulated by growth factors and other molecules. These projections may go on to form a branched tree of dendrites or a single axon or they may be reabsorbed at a later stage of development. "Neurite" may refer to any filamentous or pointed outgrowth of an embryonal or tissue-culture neural cell.
Heparan Sulfate Proteoglycans
Ubiquitous macromolecules associated with the cell surface and extracellular matrix of a wide range of cells of vertebrate and invertebrate tissues. They are essential cofactors in cell-matrix adhesion processes, in cell-cell recognition systems, and in receptor-growth factor interactions. (From Cancer Metastasis Rev 1996; 15(2): 177-86; Hepatology 1996; 24(3): 524-32)
Tumor Cells, Cultured
Cells grown in vitro from neoplastic tissue. If they can be established as a TUMOR CELL LINE, they can be propagated in cell culture indefinitely.
Receptors, Collagen
Collagen receptors are cell surface receptors that modulate signal transduction between cells and the EXTRACELLULAR MATRIX. They are found in many cell types and are involved in the maintenance and regulation of cell shape and behavior, including PLATELET ACTIVATION and aggregation, through many different signaling pathways and differences in their affinities for collagen isoforms. Collagen receptors include discoidin domain receptors, INTEGRINS, and glycoprotein VI.
Membrane Glycoproteins
Glycoproteins found on the membrane or surface of cells.
Glycoproteins
Conjugated protein-carbohydrate compounds including mucins, mucoid, and amyloid glycoproteins.
Heparin
A highly acidic mucopolysaccharide formed of equal parts of sulfated D-glucosamine and D-glucuronic acid with sulfaminic bridges. The molecular weight ranges from six to twenty thousand. Heparin occurs in and is obtained from liver, lung, mast cells, etc., of vertebrates. Its function is unknown, but it is used to prevent blood clotting in vivo and vitro, in the form of many different salts.
Epithelium
One or more layers of EPITHELIAL CELLS, supported by the basal lamina, which covers the inner or outer surfaces of the body.

Inhibition of in vitro enteric neuronal development by endothelin-3: mediation by endothelin B receptors. (1/4299)

The terminal colon is aganglionic in mice lacking endothelin-3 or its receptor, endothelin B. To analyze the effects of endothelin-3/endothelin B on the differentiation of enteric neurons, E11-13 mouse gut was dissociated, and positive and negative immunoselection with antibodies to p75(NTR )were used to isolate neural crest- and non-crest-derived cells. mRNA encoding endothelin B was present in both the crest-and non-crest-derived cells, but that encoding preproendothelin-3 was detected only in the non-crest-derived population. The crest- and non-crest-derived cells were exposed in vitro to endothelin-3, IRL 1620 (an endothelin B agonist), and/or BQ 788 (an endothelin B antagonist). Neurons and glia developed only in cultures of crest-derived cells, and did so even when endothelin-3 was absent and BQ 788 was present. Endothelin-3 inhibited neuronal development, an effect that was mimicked by IRL 1620 and blocked by BQ 788. Endothelin-3 failed to stimulate the incorporation of [3H]thymidine or bromodeoxyuridine. Smooth muscle development in non-crest-derived cell cultures was promoted by endothelin-3 and inhibited by BQ 788. In contrast, transcription of laminin alpha1, a smooth muscle-derived promoter of neuronal development, was inhibited by endothelin-3, but promoted by BQ 788. Neurons did not develop in explants of the terminal bowel of E12 ls/ls (endothelin-3-deficient) mice, but could be induced to do so by endothelin-3 if a source of neural precursors was present. We suggest that endothelin-3/endothelin B normally prevents the premature differentiation of crest-derived precursors migrating to and within the fetal bowel, enabling the precursor population to persist long enough to finish colonizing the bowel.  (+info)

Binding of the G domains of laminin alpha1 and alpha2 chains and perlecan to heparin, sulfatides, alpha-dystroglycan and several extracellular matrix proteins. (2/4299)

The C-terminal G domain of the mouse laminin alpha2 chain consists of five lamin-type G domain (LG) modules (alpha2LG1 to alpha2LG5) and was obtained as several recombinant fragments, corresponding to either individual modules or the tandem arrays alpha2LG1-3 and alpha2LG4-5. These fragments were compared with similar modules from the laminin alpha1 chain and from the C-terminal region of perlecan (PGV) in several binding studies. Major heparin-binding sites were located on the two tandem fragments and the individual alpha2LG1, alpha2LG3 and alpha2LG5 modules. The binding epitope on alpha2LG5 could be localized to a cluster of lysines by site-directed mutagenesis. In the alpha1 chain, however, strong heparin binding was found on alpha1LG4 and not on alpha1LG5. Binding to sulfatides correlated to heparin binding in most but not all cases. Fragments alpha2LG1-3 and alpha2LG4-5 also bound to fibulin-1, fibulin-2 and nidogen-2 with Kd = 13-150 nM. Both tandem fragments, but not the individual modules, bound strongly to alpha-dystroglycan and this interaction was abolished by EDTA but not by high concentrations of heparin and NaCl. The binding of perlecan fragment PGV to alpha-dystroglycan was even stronger and was also not sensitive to heparin. This demonstrated similar binding repertoires for the LG modules of three basement membrane proteins involved in cell-matrix interactions and supramolecular assembly.  (+info)

Expression of extracellular matrix proteins in cervical squamous cell carcinoma--a clinicopathological study. (3/4299)

AIM: To evaluate the intracellular and peritumoral expression of matrix proteins in squamous cell carcinoma of the uterine cervix using immunohistochemistry. METHODS: 71 squamous cell carcinomas and 10 controls were stained for laminin, fibronectin, and collagen IV. Cytoplasmic staining in tumour cells and peritumoral deposition of matrix proteins were evaluated. The association between staining results and patient age, tumour stage, histological grade, and survival was studied. RESULTS: Positive cytoplasmic staining for laminin, fibronectin, and collagen IV was observed in 17 (23.9%), 27 (38%), and 10 (14.1%) cases, respectively. Staining for laminin was most pronounced in the invasive front of tumour islands, while for fibronectin and collagen IV it appeared to be diffuse. Peritumoral staining for laminin and collagen IV was detected in 12 cases (16.9%). Early stage (Ia1-Ia2) tumours were uniformly negative for all three proteins. Cytoplasmic staining for laminin correlated with positive staining for fibronectin and collagen IV, and with the presence of a peritumoral deposition of collagen IV and laminin. There was no correlation with any of the three markers between staining results and patient age, stage, grade, or survival. CONCLUSIONS: Expression of extracellular matrix proteins in some cervical squamous cell carcinomas might reflect the enhanced ability of these tumours to modify the peritumoral stroma. This ability seems to be absent in early stage tumours. The correlation between intracytoplasmic and peritumoral expression of matrix proteins supports the evidence of their synthesis by tumour cells. However, this property did not correlate with disease outcome in this study.  (+info)

alphaSU2, an epithelial integrin that binds laminin in the sea urchin embryo. (4/4299)

At gastrulation in the sea urchin embryo dramatic cell adhesion changes contribute to primary mesenchyme cell ingression movements and to cell rearrangements during archenteron invagination. At ingression, quantitative adhesion assays demonstrated previously that primary mesenchyme cells (PMCs) change their affinity for neighboring cells, for a fibronectin-like substrate, and for the hyaline layer. To investigate the molecular basis for these and other differential cell affinities at gastrulation, we have identified an integrin that appears to be responsible for specific alterations in cell-substrate adhesion to laminin. During early cleavage stages blastomeres adhere poorly to laminin substrates. Around hatching there is a large increase in the ability of blastomeres to bind to laminin and this increase correlates temporally with the expression of an integrin on the basal surface all blastomeres. PMCs, after undergoing their epithelial-mesenchymal transition, have a strongly reduced affinity for laminin relative to ectoderm cells and, correspondingly, do not stain for the presence of the integrin. We identified the alpha integrin cDNA from Lytechinus variegatus by RT-PCR. Overlapping clones were obtained from a midgastrula cDNA library to provide a complete sequence for the integrin. The composite cDNA encoded a protein that was most similar to the alpha5 subgroup of vertebrate integrins, but there was not a definitive vertebrate integrin homolog. Northern blots and Western immunoblots showed that the sea urchin integrin, which we have named alphaSU2, is present in eggs and during all stages of development. Immunolocalization with specific polyclonal antibodies showed that alphaSU2 first appears on the basal cell surface of epithelia at the midblastula stage, at a time correlating with the increase in adhesive affinity for laminin. The protein remains at high levels on the basal surface of ectoderm cells but is temporarily reduced or eliminated from endoderm cells during their convergent-extension movements. To confirm integrin binding specificity, alphaSU2 was transfected into an alpha-integrin-deficient CHO cell line. alphaSU2-expressing CHO cells bound well to isolated sea urchin basal lamina and to purified laminin. The transfected cells bound weakly or not at all to fibronectin, type I collagen, and type IV collagen. This is consistent with the hypothesis that alphaSU2 integrin functions by binding epithelial cells to laminin in the basal lamina. In vivo, modulation of alphaSU2 integrin expression correlates with critical adhesive changes during cleavage and gastrulation. Thus, this protein appears to be an important contributor to the morphogenetic rearrangements that characterize gastrulation in the sea urchin embryo.  (+info)

The role of cell adhesion molecules in Drosophila heart morphogenesis: faint sausage, shotgun/DE-cadherin, and laminin A are required for discrete stages in heart development. (5/4299)

Heart development in the Drosophila embryo starts with the specification of cardiac precursors from the dorsal edge of the mesoderm through signaling from the epidermis. Cardioblasts then become aligned in a single row of cells that migrate dorsally. After contacting their contralateral counterparts, cardioblasts undergo a cytoskeletal rearrangement and form a lumen. Its simple architecture and cellular composition makes the heart a good system to study mesodermal patterning, intergerm layer signaling, and the function of cell adhesion molecules (CAMs) during morphogenesis. In this paper we focus on three adhesion molecules, faint sausage (fas), shotgun/DE-cadherin (shg/DE-Cad), and laminin A (lam A), that are essential for heart development. fas encodes an Ig-like CAM and is required for the correct number of cardioblasts to become specified, as well as proper alignment of cardioblasts. shg/DE-Cad is expressed and required at a later stage than fas; in embryos lacking this gene, cardioblasts are specified normally and become aligned, but do not form a lumen. Additionally, cardioblasts of shg mutant embryos show a redistribution of phosphotyrosine as well as a loss of Armadillo from the membrane, indicating defects in cell polarity. The shg phenotype could be phenocopied by applying EGTA or cytochalasin D, supporting the view that Ca2+-dependent adhesion and the actin cytoskeleton are instrumental for heart lumen formation. As opposed to cell-cell adhesion, cell-substrate adhesion mechanisms are not required for heart morphogenesis, but only for maintenance of the differentiated heart. Embryos lacking the lam A gene initially developed a normal heart, but showed twists and breaks of cardioblasts at late embryonic stages. We discuss our findings in light of recent results that elucidate the function of different adhesion systems in vertebrate heart development.  (+info)

Cell adhesion activity for murine carcinoma cells of a wheat germ 55-kDa protein with binding affinity for animal extracellular matrix proteins. (6/4299)

A wheat germ 55-kDa protein was isolated by affinity chromatography with Matrigel immobilized on agarose, followed by preparative gel electrophoresis. This Matrigel-binding protein designated as WG-55 had an amino-terminal amino acid sequence which is identical to that of a putative mature form of wheat storage protein Gbl 1. WG-55 reacted with concanavalin A, indicating its glycoprotein nature as expected from the amino acid sequence of Gbl 1. As expected, similarly, WG-55 exhibited RGD-dependent cell adhesion activity for murine carcinoma cells. These data suggest that WG-55 or mature Gbl 1 protein may play a role in plant cell adhesion.  (+info)

Development of cytotrophoblast columns from explanted first-trimester human placental villi: role of fibronectin and integrin alpha5beta1. (7/4299)

Human first-trimester floating mesenchymal villi explanted onto gels of collagen I or Matrigel were observed to undergo de novo development of anchoring sites. These consisted of cytotrophoblast columns that formed by proliferation of stem villous cytotrophoblast cells, as revealed by whole-mount and thin-section microscopy and incorporation of bromodeoxyuridine into DNA. Column formation occurred exclusively at the distal tips of the villi. No column formation was observed in tissue explanted onto agarose. On Matrigel, the developing columns penetrated downwards into the matrix, whereas on collagen I, cytotrophoblast sheets spread across the surface of the gel and merged to form a shell. The developing columnar cytotrophoblast up-regulated integrins alpha1beta1 and alpha5beta1 and produced an extracellular matrix containing oncofetal fibronectin, as in vivo. Function-blocking antibodies were used to investigate the role of the integrin-fibronectin interaction in anchoring villus development on collagen I. Antibodies to fibronectin and the integrin subunits alpha5 and beta1, added at 24 h, all changed the pattern of cytotrophoblast outgrowth. Anti-fibronectin caused cell rounding within the cytotrophoblast sheet and increased the population of single cells at its periphery. Anti-integrin alpha5 caused rounding and redistribution of cells within the outgrowth. In the presence of anti-integrin beta1, cell-collagen interactions within the sheet were destabilized, often leading to the appearance of an annulus of aggregated cells at the periphery. These results show that 1) mesenchymal villi retain the potential to form anchoring sites until at least the end of the first trimester, 2) adhesion to a permissive extracellular matrix stimulates cytotrophoblast proliferation and differentiation along the extravillous lineage, 3) integrin alpha5beta1-fibronectin interactions contribute significantly to anchorage of the placenta to uterine extracellular matrix. We suggest that as the developing placenta ramifies, new sites of anchorage form whenever peripheral villi contact decidua. This process is predicted to contribute to the stability of the placental-decidual interface.  (+info)

Extracellular matrix regulates apoptosis in mammary epithelium through a control on insulin signaling. (8/4299)

Adherent epithelial cells require interactions with the extracellular matrix for their survival, though the mechanism is ill-defined. In long term cultures of primary mammary epithelial cells, a laminin-rich basement membrane (BM) but not collagen I suppresses apoptosis, indicating that adhesion survival signals are specific in their response (. J. Cell Sci. 109:631-642). We now demonstrate that the signal from BM is mediated by integrins and requires both the alpha6 and beta1 subunits. In addition, a hormonal signal from insulin or insulin-like growth factors, but not hydrocortisone or prolactin, is necessary to suppress mammary cell apoptosis, indicating that BM and soluble factors cooperate in survival signaling. Insulin induced autophosphorylation of its receptor whether mammary cells were cultured on collagen I or BM substrata. However, both the tyrosine phosphorylation of insulin receptor substrate-1 and its association with phosphatidylinositol 3-kinase were enhanced in cells cultured on BM, as was the phosphorylation of the phosphatidylinositol 3-kinase effector, protein kinase B. These results suggest a novel extracellular matrix-dependent restriction point in insulin signaling in mammary epithelial cells. The proximal signal transduction event of insulin receptor phosphorylation is not dependent on extracellular matrix, but the activation of downstream effectors requires adhesion to BM. Since phosphatidylinositol 3-kinase was required for mammary epithelial cell survival, we propose that a possible mechanism for BM-mediated suppression of apoptosis is through its facilitative effects on insulin signaling.  (+info)

Galectin-3, a laminin binding protein, fails to modulate adhesion of human melanoma cells to laminin<...
TY - JOUR. T1 - Galectin-3, a laminin binding protein, fails to modulate adhesion of human melanoma cells to laminin. AU - Van Den Brule, F. A.. AU - Buicu, C.. AU - Sobel, M. E.. AU - Liu, Fu-Tong. AU - Castronovo, V.. PY - 1995. Y1 - 1995. N2 - Galectin-3 is a laminin binding protein which expression is altered in a variety of human carcinomas including colon, breast and endometrium. In these tumors, we consistently observed a down regulation of galectin-3 expression related to increased aggressiveness. Galectin-3 belongs to a family of galactose-binding lectins and binds laminin through its numerous poly-N-acetyllactosamine chains. To date, the exact role of galectin-3 in the complex interactions between cancer cells and laminin has not been clearly defined. Adhesion of melanoma cells to laminin is a critical event during tumor invasion and metastasis. In this study, we explore the possibility that galectin-3 could modulate attachment of two human melanoma cell lines to laminin. A2058 and ...
https://ucdavis.pure.elsevier.com/en/publications/galectin-3-a-laminin-binding-protein-fails-to-modulate-adhesion-o
Laminin α subunits and their role in C. elegans development<...Laminin α subunits and their role in C. elegans development<...
TY - JOUR. T1 - Laminin α subunits and their role in C. elegans development. AU - Huang, Cheng Chen. AU - Hall, David H.. AU - Hedgecock, Edward M.. AU - Kao, Gautam. AU - Karantza, Vassiliki. AU - Vogel, Bruce E.. AU - Hutter, Harald. AU - Chisholm, Andrew D.. AU - Yurchenco, Peter D.. AU - Wadsworth, William G.. PY - 2003/7/1. Y1 - 2003/7/1. N2 - Laminins are heterotrimeric (α/β/γ) glycoproteins that form a major polymer within basement membranes. Different α, β and γ subunits can assemble into various laminin isoforms that have different, but often overlapping, distributions and functions. In this study, we examine the contributions of the laminin α subunits to the development of C. elegans. There are two α, one β and one γ laminin subunit, suggesting two laminin isoforms that differ by their α subunit assemble in C. elegans. We find that near the end of gastrulation and before other basement membrane components are detected, the α subunits are secreted between primary tissue ...
https://einstein.pure.elsevier.com/en/publications/laminin-%CE%B1-subunits-and-their-role-in-c-elegans-development-2
Anti Human Laminin Alpha 2 Antibody, clone 5H2 | Bio-RadAnti Human Laminin Alpha 2 Antibody, clone 5H2 | Bio-Rad
|strong|Mouse anti Human laminin alpha 2 antibody, clone 5H2|/strong| recognizes the ~80 kDa fragment of laminin subunit alpha-2, also known as the laminin M chain, laminin-12 subunit alpha, lami…
https://www.bio-rad-antibodies.com/monoclonal/human-laminin-antibody-5h2-6067-0906.html
A synaptic localization domain in the synaptic cleft protein laminin beta 2 (s-laminin) | ScienceA synaptic localization domain in the synaptic cleft protein laminin beta 2 (s-laminin) | Science
The basal lamina that ensheaths skeletal muscle fibers traverses the synaptic cleft at the neuromuscular junction. Synaptic and extrasynaptic portions of the basal lamina contain different laminin beta chains: beta 2 (or s) at synapses and beta 1 (or B1) extrasynaptically. Laminin beta 2 is also confined to synapselike patches on myotube surfaces in vitro, whereas beta 1 is present throughout the extracellular matrix. This differential localization of laminin beta chains was analyzed by expression of chimeric beta 1-beta 2 molecules in cultured mouse myotubes. A 16-amino acid carboxyl-terminal sequence in beta 2 was necessary for synaptic localization, and an amino-terminal domain in beta 1 promoted association with extracellular fibrils. The synaptic targeting sequence of beta 2 contains a site previously shown to be adhesive for motor neurons. ...
http://science.sciencemag.org/content/269/5222/413
A novel laminin alpha2 isoform in severe laminin alpha2 deficient congenital muscular dystrophy.
Laminin alpha2 deficiency presents at birth with muscle weakness, hypotonia, and usually asymptomatic white matter signal on MRI. Few patients with laminin alpha2 deficiency have been described with seizures and structural brain abnormalities. The re
http://www.biomedsearch.com/nih/novel-laminin-alpha2-isoform-in/11071490.html
The Short Arm of the Laminin γ2 Chain Plays a Pivotal Role in the Incorporation of Laminin 5 into the Extracellular Matrix and...The Short Arm of the Laminin γ2 Chain Plays a Pivotal Role in the Incorporation of Laminin 5 into the Extracellular Matrix and...
Transfection of a wild-type laminin γ2 cDNA in LSV5 keratinocytes rescues expression of functional laminin 5 molecules (Gagnoux-Palacios et al. 1996). Similar to laminin 5 produced by wild-type keratinocytes, the recombinant laminin 5 secreted by the reverted LSV5 cells undergoes extracellular maturation, including proteolytic excision of the NH2-terminal domains of the γ2 short arm. In this study, we examined the functional role of the laminin γ2 short arm by complementing the genetic defect of LSV5 cells by transfer of mutant γ2 cDNAs. Expression of the mutant cDNAs demonstrated that all the different recombinant γ2 chains associate with the endogenous laminin α3 and β3 polypeptides to assemble into α3β3γ2 heterotrimers, and that the NH2-terminal domains of the γ2 chain are required for deposition and incorporation of laminin 5 in the ECM.. Our results, for the first time, define a physiologic function for the short arm of the γ2 chain. We demonstrate that mutant laminin 5 ...
http://jcb.rupress.org/content/153/4/835.long
Laminin in the male germ cells of Drosophila. | JCBLaminin in the male germ cells of Drosophila. | JCB
To study genes that may be crucial for the male germ cell development of Drosophila we screened a cDNA expression library with a polyclonal antiserum against testis proteins of Drosophila hydei. We identified a cDNA fragment that exhibited a complete sequence similarity with the cDNA of the laminin B2 chain, an important component of the extracellular matrix. Transcripts of laminin B2 were detected in the RNA of male germ cells with the polymerase chain reaction and by in situ hybridization. We studied the reaction of different polyclonal antibodies including those against a Drosophila laminin B2-lac fusion protein, the entire Drosophila laminin complex, or against the mouse laminin complex and against laminin A and B1 chains with specific structures in developing male germ cells of Drosophila. Antigenic sites against laminin B2 were found in the lampbrush loops in primary spermatocyte nuclei, in nuclei of spermatids, and in heads of spermatozoa. The axonemes of elongating spermatids react with ...
http://jcb.rupress.org/content/119/4/977
Dystroglycan loss disrupts polarity and beta-casein induction in mammary epithelial cells by perturbing laminin anchoring. -...Dystroglycan loss disrupts polarity and beta-casein induction in mammary epithelial cells by perturbing laminin anchoring. -...
Precise contact between epithelial cells and their underlying basement membrane is crucial to the maintenance of tissue architecture and function. To understand the role that the laminin receptor dystroglycan (DG) plays in these processes, we assayed cell responses to laminin-111 following conditional ablation of DG gene (Dag1) expression in cultured mammary epithelial cells. Strikingly, DG loss disrupted laminin-111-induced polarity and beta-casein production, and abolished laminin assembly at the step of laminin binding to the cell surface. Dystroglycan re-expression restored these deficiencies. Investigations of the mechanism revealed that DG cytoplasmic sequences were not necessary for laminin assembly and signaling, and only when the entire mucin domain of extracellular DG was deleted did laminin assembly not occur. These results demonstrate that DG is essential as a laminin-111 co-receptor in mammary epithelial cells that functions by mediating laminin anchoring to the cell surface, a process that
https://www.semanticscholar.org/paper/Dystroglycan-loss-disrupts-polarity-and-beta-casei-Weir-Oppizzi/f3cbdae2b82f2730a12de5c79dc5919888a787ac
Mild clinical phenotype in a 12-year-old boy with partial merosin deficiency and central and peripheral nervous system...
TY - JOUR. T1 - Mild clinical phenotype in a 12-year-old boy with partial merosin deficiency and central and peripheral nervous system abnormalities. AU - Mora, Marina. AU - Moroni, Isabella. AU - Uziel, Graziella. AU - Di Blasi, Claudia. AU - Barresi, Rita. AU - Farina, Laura. AU - Morandi, Lucia. PY - 1996/10. Y1 - 1996/10. N2 - We found partial merosin deficiency in a boy presenting at 12 yr with marked limb weakness and a waddling gait. Magnetic resonance imaging (MRI) showed the characteristic white matter abnormalities of merosin-negative congenital muscular dystrophy. There were also peripheral demyelinating polyneuropathy and evoked potential abnormalities. Unlike classic merosin-negative congenital muscular dystrophy, however, our patient was less hypotonic and weak and was able to achieve independent walking. Both by immunohistochemistry and Western blot merosin was shown to be moderately reduced. By immunostaining the α1 laminin chain was overexpressed and the β1 laminin chain was ...
https://moh-it.pure.elsevier.com/en/publications/mild-clinical-phenotype-in-a-12-year-old-boy-with-partial-merosin
Macrophage interactions with laminin: PMA selectively induces the adhe by Arthur M. Mercurio and Leslie M. ShawMacrophage interactions with laminin: PMA selectively induces the adhe by Arthur M. Mercurio and Leslie M. Shaw
The ability of thioglycollate (TG)-elicited mouse peritoneal macrophages to adhere to a laminin substratum has been studied. These cells do not adhere to laminin-coated (20 micrograms/ml) surfaces, but the addition of phorbol myristate acetate (PMA; 50 ng/ml) results in their rapid adherence and spreading on this substratum. TG-elicited and PMA-activated macrophages, however, can bind soluble laminin. Macrophages adhere to fibronectin-coated surfaces and tissue culture plastic without PMA stimulation, and PMA does not increase the number of cells that adhere to these surfaces. The predominant surface proteins that bind specifically to laminin-Sepharose exhibit an Mr of 67 and 36 kD, but the expression of these proteins does not increase after PMA stimulation. Laminin receptor antibodies immunoprecipitate the 67-kD protein from radiolabled surface lysates and are capable of blocking macrophage adherence to a laminin substratum. Indirect immunofluorescence microscopy indicates that PMA stimulation does
https://escholarship.umassmed.edu/cancerbiology_pp/111/
Laminin, alpha 4 - WikipediaLaminin, alpha 4 - Wikipedia
Laminins, a family of extracellular matrix glycoproteins, are the major noncollagenous constituent of basement membranes. They have been implicated in a wide variety of biological processes including cell adhesion, differentiation, migration, signaling, neurite outgrowth and metastasis. Laminins are composed of 3 non identical chains: laminin alpha, beta and gamma (formerly A, B1, and B2, respectively) and they form a cruciform structure consisting of 3 short arms, each formed by a different chain, and a long arm composed of all 3 chains. Each laminin chain is a multidomain protein encoded by a distinct gene. Several isoforms of each chain have been described. Different alpha, beta and gamma chain isomers combine to give rise to different heterotrimeric laminin isoforms which are designated by Arabic numerals in the order of their discovery, i.e. alpha1beta1gamma1 heterotrimer is laminin 1. The biological functions of the different chains and trimer molecules are largely unknown, but some of the ...
https://en.wikipedia.org/wiki/Laminin,_alpha_4
Structural analysis of a human glial variant laminin<...
TY - JOUR. T1 - Structural analysis of a human glial variant laminin. AU - LeMosy, Ellen. AU - Lightner, Virginia A.. AU - Erickson, Harold P.. PY - 1996/8/25. Y1 - 1996/8/25. N2 - Astrocytes secrete laminin-like molecules in culture and may represent a major source of laminin in the developing central nervous system, yet these laminins have not been extensively characterized. We previously reported the presence of an astrocyte-derived variant laminin in media conditioned by human U251 MG astrocytoma cells. This laminin was partially purified in a highly anionic Mono Q fraction with strong adhesion activity for fibroblasts and glial cells (Aukhil et al. (1990) Matrix 10: 98-111). We now show that glial laminin could be dissociated from an anionic species, perhaps an ~400- kDa keratan sulfate proteoglycan present in the preparation, by a second round of Mono Q anion exchange chromatography in the presence of 6 M urea. Cell adhesion activity remained tightly associated with laminin-containing ...
https://augusta.pure.elsevier.com/en/publications/structural-analysis-of-a-human-glial-variant-laminin
Mutagenetix > Incidental...Mutagenetix > Incidental...
FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes one of the vertebrate laminin alpha chains. Laminins, a family of extracellular matrix glycoproteins, are the major noncollagenous constituent of basement membranes. They have been implicated in a wide variety of biological processes including cell adhesion, differentiation, migration, signaling, neurite outgrowth and metastasis. Laminins are composed of 3 non identical chains: laminin alpha, beta and gamma (formerly A, B1, and B2, respectively) and they form a cruciform structure consisting of 3 short arms, each formed by a different chain, and a long arm composed of all 3 chains. Each laminin chain is a multidomain protein encoded by a distinct gene. The protein encoded by this gene is the alpha-5 subunit of of laminin-10 (laminin-511), laminin-11 (laminin-521) and laminin-15 (laminin-523). [provided by RefSeq, Jun 2013 ...
https://mutagenetix.utsouthwestern.edu/incidental/incidental_rec.cfm?mid=660474
Laminin S/Laminin beta 2 Proteins: Novus BiologicalsLaminin S/Laminin beta 2 Proteins: Novus Biologicals
Laminin S/Laminin beta 2 Proteins available through Novus Biologicals. Browse our Laminin S/Laminin beta 2 Protein catalog backed by our Guarantee+.
https://www.novusbio.com/proteins/laminin-s-laminin-beta-2?related_diseases=Nephrotic%20Syndrome
Laminin S/Laminin beta 2 Proteins: Novus BiologicalsLaminin S/Laminin beta 2 Proteins: Novus Biologicals
Laminin S/Laminin beta 2 Proteins available through Novus Biologicals. Browse our Laminin S/Laminin beta 2 Protein catalog backed by our Guarantee+.
https://www.novusbio.com/proteins/laminin-s-laminin-beta-2?showGrid=true
Immune responses against aldehyde-modified laminin accelerate atherosclerosis in Apoe(-/-) mice. | Lund University Diabetes...Immune responses against aldehyde-modified laminin accelerate atherosclerosis in Apoe(-/-) mice. | Lund University Diabetes...
BACKGROUND: LDL oxidation in the vascular wall is associated with aldehyde modification of surrounding extracellular matrix proteins that may target autoimmune responses against vascular tissues. Here we investigated the possible influence of immunity against a malondialdehyde (MDA)-modified form of the basement membrane protein laminin on atherosclerosis. METHODS AND RESULTS: IgM and IgG autoantibodies were present in human plasma and a prospective clinical study demonstrated that individuals who later suffered from acute cardiovascular events had lower levels of MDA-laminin antibodies compared to those in the control group. Immunohistochemical analysis of atherosclerotic plaques from Apoe(-/-) mice demonstrated co-localization between laminin and MDA epitopes, however MDA-laminin IgG was absent in mouse plasma. To determine the effect of MDA-laminin immunity, Apoe(-/-) mice were immunized with MDA-laminin. Analysis of circulating leukocytes at 12 weeks demonstrated increased T-cell activation, ...
https://www.ludc.lu.se/fong-to/publication/eff31a2c-1e6a-4860-90b3-21d58a806d6b
Endogenous laminin is required for human airway smooth muscle cell maturation | Respiratory Research | Full TextEndogenous laminin is required for human airway smooth muscle cell maturation | Respiratory Research | Full Text
Airway smooth muscle (ASM) contraction underlies acute bronchospasm in asthma. ASM cells can switch between a synthetic-proliferative phenotype and a contractile phenotype. While the effects of extracellular matrix (ECM) components on modulation of ASM cells to a synthetic phenotype have been reported, the role of ECM components on maturation of ASM cells to a contractile phenotype in adult lung is unclear. As both changes in ECM components and accumulation of contractile ASM are features of airway wall remodelling in asthma, we examined the role of the ECM protein, laminin, in the maturation of contractile phenotype in human ASM cells. Human ASM cells were made senescence-resistant by stable expression of human telomerase reverse transcriptase. Maturation to a contractile phenotype was induced by 7-day serum deprivation, as assessed by immunoblotting for desmin and calponin. The role of laminin on ASM maturation was investigated by comparing the effects of exogenous laminin coated on culture plates,
https://respiratory-research.biomedcentral.com/articles/10.1186/1465-9921-7-117/metrics
Endogenous laminin is required for human airway smooth muscle cell maturation | Respiratory Research | Full TextEndogenous laminin is required for human airway smooth muscle cell maturation | Respiratory Research | Full Text
Airway smooth muscle (ASM) contraction underlies acute bronchospasm in asthma. ASM cells can switch between a synthetic-proliferative phenotype and a contractile phenotype. While the effects of extracellular matrix (ECM) components on modulation of ASM cells to a synthetic phenotype have been reported, the role of ECM components on maturation of ASM cells to a contractile phenotype in adult lung is unclear. As both changes in ECM components and accumulation of contractile ASM are features of airway wall remodelling in asthma, we examined the role of the ECM protein, laminin, in the maturation of contractile phenotype in human ASM cells. Human ASM cells were made senescence-resistant by stable expression of human telomerase reverse transcriptase. Maturation to a contractile phenotype was induced by 7-day serum deprivation, as assessed by immunoblotting for desmin and calponin. The role of laminin on ASM maturation was investigated by comparing the effects of exogenous laminin coated on culture plates,
https://respiratory-research.biomedcentral.com/articles/10.1186/1465-9921-7-117/tables/1
Cartilage cellsCartilage cells
Laminins are continuously expressed during all developing stages of cartilage and cartilage-like tissues and show a region-specific expression pattern. In cartilage from newborn mice, basement membrane components are widespread in the territorial and interterritorial matrix, while in mature cartilage of adult mice the basement membrane components are localized mainly to a narrow pericellular zone around the chondrocytes (Kvist, 2008). Of the laminin subunits surveyed, α1, α5, β1, and γ1 have shown the highest staining intensity (Kvist, 2008). Lee and colleagues found that laminin chains (α1, α2, β1, β2, and γ1), produced by chicken embryo sternal chondrocytes, exhibited an increased expression in aggregated cells during the maturation stage (Lee, 1997). In newborn mouse knee cartilage, laminin 111 showed staining in the extracellular matrix, while laminin α5 (511 and 521) stained weakly at this point (Kvist, 2008). The laminin β1 chain is expressed from gestational weeks (gw) 10 ...
http://www.biolamina.com/cartilage-cells
Larger Than Life: LAMiNiN.Larger Than Life: LAMiNiN.
After the game, we had FCA, its the bomb, and Mr. Sheek talked about Laminin. Laminin by definition is the basement membrane, which is a protein network foundation for most cells and organs. Now youre wondering why in the world you should keep reading this, and why i am telling you this. Well Im here to tell you that Laminin is what our bodies is made up of. Scripture says that God is in us. And people wonder how in the world can God be In us. Well Laminin is proof. Laminin is, well let me show you what Laminin is. ...
http://largerthanlife-hannah.blogspot.com/2009/09/laminin.html
CorningCorning
Corning™ rLaminin-521 (Human) is an animal-component-free recombinant heterotrimer composed of a5, B2, and y1 chains expressed in a mammalian cell culture system. The Corning rLaminin-521 (Human) surface supports such key benefits as ROCK inhibitor independent, single-cell expansion of PSCs and inhibition of spontaneous differentiation, improving hPSC culture ease and efficiency.. A collaboration focused on bringing together better solutions for stem cell research. Corning has partnered with BioLamina for the supply of recombinant human laminin 521 (rLaminin-521), a next generation surface that enables feeder-free, single cell passaging of pluripotent stem cells (PSCs) without ROCK-inhibitor. Now weve gone one step further and are working together to combine BioLaminas expertise in recombinant laminin expression with Cornings extensive line of vessels and proprietary surface coating technologies. This partnership brings robust and scalable expansion solutions to the emerging stem cell ...
https://www.fishersci.se/se/en/brands/IL1ZGMA2/corning.html?utm_campaign=12367_Corning_NutriStem&utm_medium=web&utm_source=fp&utm_content=fp_banner
rLamininrLaminin
Corning has partnered with BioLamina for the supply of recombinant human laminin 521 (rLaminin-521), a next generation surface that enables feeder-free, single cell passaging of pluripotent stem cells (PSCs) without ROCK-inhibitor. Now weve gone one step further and are working together to combine BioLaminas expertise in recombinant laminin expression with Cornings extensive line of vessels and proprietary surface coating technologies. This partnership brings robust and scalable expansion solutions to the emerging stem cell processing market, enabling our customers to scale-up effectively and mitigate risk.. Now backed by Cornings large network, worldwide distribution channels, and cell culture portfolio, researchers globally have better access and availability to rLaminin-521 (Human) than ever before. rLaminin-521 (Human) joins the Corning ECM mimetic and advanced surfaces family: animal component-free surfaces suited for stem and progenitor cell expansion under cGMP processing environments. ...
https://www.corning.com/emea/de/products/life-sciences/products/surfaces/rlaminin.html
Talk:2015 Group 5 Project - CellBiologyTalk:2015 Group 5 Project - CellBiology
Z3461763 (talk) 16:15, 16 April 2015 (EST) Ill upload mine when I get home because its saved on my usb... Its completed just havent uploaded. :D --Z3461763 (talk) 21:16, 16 April 2015 (EST) is there a reason why anything im trying to upload wont upload? its been an hour and nothing will save! --Z3461763 (talk) 23:16, 16 April 2015 (EST) The Heterotrimeric Laminin Coiled - Coil Domain Exerts Anti - Adhesive Effects and Induces a Pro - Invasive Phenotype Laminins are a family of ECM glycoproteins localised in the basement membrane. As well as their structural roles, they also regulate cellular processes. These include cell migration, differentiation and proliferation. Laminins also have multiple binding partners; hence, the study was to determine what would happen to individual truncated Laminin chains in the absence of their normal partners. They did this by selecting a transfectable cell line with low endogenous Laminin chain expression, by first encoding the truncated Laminin α1, β1 ...
https://cellbiology.med.unsw.edu.au/cellbiology/index.php?title=Talk:2015_Group_5_Project&oldid=57279
Difference between revisions of Talk:2015 Group 5 Project - CellBiologyDifference between revisions of Talk:2015 Group 5 Project - CellBiology
Z3461763 (talk) 16:15, 16 April 2015 (EST) Ill upload mine when I get home because its saved on my usb... Its completed just havent uploaded. :D --Z3461763 (talk) 21:16, 16 April 2015 (EST) is there a reason why anything im trying to upload wont upload? its been an hour and nothing will save! --Z3461763 (talk) 23:16, 16 April 2015 (EST) The Heterotrimeric Laminin Coiled - Coil Domain Exerts Anti - Adhesive Effects and Induces a Pro - Invasive Phenotype Laminins are a family of ECM glycoproteins localised in the basement membrane. As well as their structural roles, they also regulate cellular processes. These include cell migration, differentiation and proliferation. Laminins also have multiple binding partners; hence, the study was to determine what would happen to individual truncated Laminin chains in the absence of their normal partners. They did this by selecting a transfectable cell line with low endogenous Laminin chain expression, by first encoding the truncated Laminin α1, β1 ...
https://cellbiology.med.unsw.edu.au/cellbiology/index.php?title=Talk:2015_Group_5_Project&diff=57278&oldid=57277&printable=yes
Genetic variation in a member of the laminin gene family affects variation in body composition in Drosophila and humans - pdf...
Genetic variation in a member of the laminin gene family affects variation in body composition in Drosophila and humans. . Biblioteca virtual para leer y descargar libros, documentos, trabajos y tesis universitarias en PDF. Material universiario, documentación y tareas realizadas por universitarios en nuestra biblioteca. Para descargar gratis y para leer online.
http://libros.duhnnae.com/2017/jul5/150019907524-Genetic-variation-in-a-member-of-the-laminin-gene-family-affects-variation-in-body-composition-in-Drosophila-and-humans.php
RT² qPCR Primer Assay for Human LAMB2RT² qPCR Primer Assay for Human LAMB2
Laminins, a family of extracellular matrix glycoproteins, are the major noncollagenous constituent of basement membranes. They have been implicated in a wide variety of biological processes including cell adhesion, differentiation, migration, signaling, neurite outgrowth and metastasis. Laminins are composed of 3 non identical chains: laminin alpha, beta and gamma (formerly A, B1, and B2, respectively) and they form a cruciform structure consisting of 3 short arms, each formed by a different chain, and a long arm composed of all 3 chains. Each laminin chain is a multidomain protein encoded by a distinct gene. Several isoforms of each chain have been described. Different alpha, beta and gamma chain isomers combine to give rise to different heterotrimeric laminin isoforms which are designated by Arabic numerals in the order of their discovery, i.e. alpha1beta1gamma1 heterotrimer is laminin 1. The biological functions of the different chains and trimer molecules are largely unknown, but some of the ...
http://www.sabiosciences.com/primerinfo.php?pcatn=PPH20901A
Mutagenetix > Incidental...Mutagenetix > Incidental...
FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] Laminins, a family of extracellular matrix glycoproteins, are the major noncollagenous constituent of basement membranes. They have been implicated in a wide variety of biological processes including cell adhesion, differentiation, migration, signaling, neurite outgrowth and metastasis. Laminins are composed of 3 non identical chains: laminin alpha, beta and gamma (formerly A, B1, and B2, respectively) and they form a cruciform structure consisting of 3 short arms, each formed by a different chain, and a long arm composed of all 3 chains. Each laminin chain is a multidomain protein encoded by a distinct gene. Several isoforms of each chain have been described. Different alpha, beta and gamma chain isomers combine to give rise to different heterotrimeric laminin isoforms which are designated by Arabic numerals in the order of their discovery, i.e. alpha1beta1gamma1 heterotrimer is laminin 1. The ...
https://mutagenetix.utsouthwestern.edu/incidental/incidental_rec.cfm?mid=292270
Anti-Laminin AntibodyAnti-Laminin Antibody
Monoclonal clone# G2 antibody for LAMA1; LAMA2; LAMB1; LAMB2 detection. Host: Mouse.Size: 100μg/vial. Tested applications: IHC-P. Reactive species: Feline. LAMA1; LAMA2; LAMB1; LAMB2 information: Molecular Weight: 196474 MW; Subcellular Localization: Sec
https://www.bosterbio.com/anti-laminin-antibody-monoclonal-ma1054.html
Laminin (human) AlphaLISA Detection Kit, 500 Assay Points | PerkinElmerLaminin (human) AlphaLISA Detection Kit, 500 Assay Points | PerkinElmer
The AlphaLISA® immunoassay kit for human laminin enables the detection and quantitation of human laminin in human serum, plasma, lysates, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps). The assay shows no cross-reactivity with human IL-28A, IL-29, and Mouse Laminin.
http://www.perkinelmer.com/product/alphalisa-laminin-kit-500pts-al368c
Obilježja bazalne membrane u neinvazivnom duktalnom karcinomu dojke | Repozitorij Prirodoslovno-matematičkog fakultetaObilježja bazalne membrane u neinvazivnom duktalnom karcinomu dojke | Repozitorij Prirodoslovno-matematičkog fakulteta
In this work we analyzed data obtained from case-history of 36 patients operated in Hospital for tumors, Zagreb in period 2006 till 2007 year. Ductal breast cancer in situ (DCIS) were diagnosed in 24 patients while in 12 patients diagnosis was ductal micro invasive breast cancer. Aim of thesis was to define expression of polyclonal and monoclonal anti-laminin antibodies on basement membrane of ducts in breast cancer using immunohistochemical method. We investigated whether loss of laminin in basement membrane can be a predictive marker for micro invasiveness. We also investigated association between prognostic factors as age of patients, tumor differentiation stage, steroid hormone receptors and HER2 status with anti-laminin expression and micro invasion appearance. Average age of patients was 56.5 years. The results showed that polyclonal anti-laminin is an important predictive factor of micro invasion appearance in DCIS (p=0,00026) comparing to monoclonal anti-laminin (p=0,23765) (p,0,05 ). ...
https://repozitorij.pmf.unizg.hr/islandora/object/pmf:3566
Connection | Profiles RNS
SMART: Pfam domain Laminin ISMART: Pfam domain Laminin I
Laminins are glycoproteins that are major constituents of the basement membrane of cells. Laminins are trimeric molecules; laminin-1 is an alpha1 beta1 gamma1 trimer. It has been suggested that the domains I and II from laminin A, B1 and B2 may come together to form a triple helical coiled-coil structure [(PUBMED:3182802)]. Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organisation of cells into tissues during embryonic development by interacting with other extracellular matrix components.. ...
http://smart.embl.de/smart/do_annotation.pl?DOMAIN=Pfam:Laminin_I&
PROSITEPROSITE
Laminin is a large molecular weight glycoprotein present only in basement membranes in almost every animal tissue. Each laminin is a heterotrimer assembled from α, β and γ chain subunits, secreted and incorporated into cell-associated extracellular matrices. The laminins can self-assemble, bind to other matrix macromolecules, and have unique and shared cell interactions mediated by integrins, dystroglycan, and other receptors. Through these interactions, laminins critically contribute to cell differentiation, shape and movement, maintenance of tissue phenotypes, and promotion of tissue survival [1,2]. The different laminin chains share a 600-residue domain I/II which oligomerizes into a rod-like coiled-coil structure forming the long arm of laminins. The N-terminal short arms consist of rod-like elements (domain III and V) formed by tandem arrays of laminin-type EGF modules (see ,PDOC00961,) and several globular domains: domains IV and domain VI (laminin N-terminal) (see ,PDOC51117,). All α ...
https://prosite.expasy.org/doc/PS51116
Laminin, alpha 2 - wikidocLaminin, alpha 2 - wikidoc
Laminin, an extracellular matrix protein, is a major component of the basement membrane. It is thought to mediate the attachment, migration, and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components. It is composed of three subunits, alpha, beta, and gamma, which are bound to each other by disulfide bonds into a cross-shaped molecule. This gene encodes the alpha 2 chain, which constitutes one of the subunits of laminin 2 (merosin) and laminin 4 (s-merosin). Mutations in this gene have been identified as the cause of congenital merosin-deficient muscular dystrophy. Two transcript variants encoding different proteins have been found for this gene.[3] ...
https://www.wikidoc.org/index.php/Laminin,_alpha_2
RE: [Histonet] Laminin antibody
We use the same antibody at 1:20 with proteinase k from DAKO for 10min at RT. Juan C. Gutierrez, HT(ASCP) Histology Laboratory Supervisor (210)704-2533 My opinions are my own and do not reflect those of my employer. Long live free speech! -----Original Message----- From: [email protected] [mailto:[email protected]] On Behalf Of Coolen, Neeltje Sent: Thursday, April 07, 2005 3:37 AM To: [email protected] Subject: [Histonet] Laminin antibody Dear Histonetters, Are there any people who have experience with the following anti-laminin antibody: mouse monoclonal, clone 4C7 from DAKO (code no: M0638)? We tried to use this antibody on paraffin-embedded human skin tissue (formalin-fixed), but it did not work. We tried several antigen retrieval procedures, like pepsin, proteinase K and trypsin digestion, but all of these fail. Does anyone know an appropriate antigen retrieval or should I try another antibody? Thank you for your help. Neeltje ...
http://www.histosearch.com/histonet/Apr05/RE.HistonetLamininantibodB.html
SMART: EGF domain annotationSMART: EGF domain annotation
Multidomain proteins of the extracellular matrix (ECM) play an important role in development and maintenance of cellular organization and in tissue repair. Several ECM proteins such as laminin, tenascin and thrombospondin contain domains with homology to epidermal growth factor (EGF) and exhibit growth promoting activity. The mitogenic activity of laminin is restricted to a fragment which consists of about 25 repeating domains with partial homology to EGF and comprises the rod-like inner regions of the three short arms of the four armed molecule. The mitogenic activity does not correlate with promotion of cell attachment and neurite outgrowth for which major functional sites have been found in other regions of the laminin molecule. It is suggested that EGF-like domains in laminin, in other ECM proteins and in the extracellular portions of some membrane proteins are signals for cellular growth and differentiation. Because they are integral parts of large molecules and often of supramolecular ...
http://smart.embl.de/smart/do_annotation.pl?DOMAIN=EGF&START=1293&END=1326&E_VALUE=1.13e1&TYPE=SMART&BLAST=SCRELQCPVGIPCQQTARGPRCACPPGLSGPSCR
Involvement of activator protein 1 complexes in the epithelium-specific activation of the laminin γ2-chain gene promoter by...Involvement of activator protein 1 complexes in the epithelium-specific activation of the laminin γ2-chain gene promoter by...
Laminin-5 is a trimer of laminin α3, β3 and γ2 chains that is found in the intestinal basement membrane. Deposition of the laminin γ2 chain at the basement membrane is of great interest because it undergoes a developmental shift in its cellular expression. Here we study the regulatory elements that control basal and cytokine-activated transcriptional expression of the LAMC2 gene, which encodes the laminin γ2 chain. By using transient transfection experiments we demonstrated the presence of constitutive and cytokine-responsive cis-elements. Comparison of the transcriptional activity of the LAMC2 promoter in the epithelial HT29mtx cells with that in small-intestinal fibroblastic cells (C20 cells) led us to conclude that two regions with constitutive epithelium-specific activity are present between positions -1.2 and -0.12 kb. This was further validated by transfections of primary foetal intestinal endoderm and mesenchyme. A 2.5 kb portion of the LAMC2 5ʹ flanking region was equally ...
http://www.biochemj.org/content/347/2/407
anti Laminin, alpha 3 LAMA3 - Journal for the International Association for Laboratory Learning and Technologyanti Laminin, alpha 3 LAMA3 - Journal for the International Association for Laboratory Learning and Technology
Description: A polyclonal antibody for detection of Laminin Alpha-3 from Human, Mouse, Rat. This Laminin Alpha-3 antibody is for IHC-P, IF, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the C-terminal region of human Laminin ?-3 at AA rangle: 2540- ...
https://ialltjournal.org/anti-laminin-alpha-3-lama3/
Laminin beta 1抗体|Abcam中国|Anti-Laminin beta 1抗体(ab69633)Laminin beta 1抗体|Abcam中国|Anti-Laminin beta 1抗体(ab69633)
Laminin beta 1兔多克隆抗体(ab69633)可与人样本反应并经WB, ELISA, IHC, ICC/IF实验严格验证。所有产品均提供质保服务，中国75%以上现货。
http://www.abcam.cn/Laminin-beta-1-antibody-ab69633.html
Available Positions | The Fass LabAvailable Positions | The Fass Lab
Laminin is a major component of the extracellular matrix (ECM) and arose early in animal evolution. Due to its role in cell adhesion and migration, laminin is central to development and disease. Laminin is a heterotrimer with a total mass of up to 800 kD. Its three subunits (α, β, and γ) assemble into a distinctive cross-shaped structure, copies of which further self-assemble to form a network. Higher organisms contain a number of isotypes of each subunit, which produce, combinatorially, a rich family of related proteins with certain structural and functional distinctions. Many fundamental aspects of laminin structure are understood from rotary shadowing electron microscopy, recognizable amino acid sequence motifs (i.e., coiled-coil heptad repeats), and X-ray crystallography of laminin fragments. However, other conserved portions of laminin, namely the L4, LF, and Lβ knob domains, are still poorly understood, both structurally and functionally. The L4 and LF domains are ...
http://www.weizmann.ac.il/Structural_Biology/Fass/positions
LamininLaminin
Use Corning Laminin surfaces as a thin coating on tissue culture surfaces or as a soluble additive to culture medium to promote cell adhesion, migration, chemotaxis, growth, and differentiation. Laminin/Entactin Complex High Concentration (HC) is a special formulation that Corning has developed to enhance three-dimensional (3D) cell culture applications. Culturing cells in or on gels such as Laminin/Entactin Complex enable the study of specific mechanisms that dictate cell differentiation and functionality.. Applications include:. ...
http://www.corning.com/cala/es/products/life-sciences/products/surfaces/laminin.html
Laminin抗体[MEC5]|Abcam中国|Anti-Laminin抗体[MEC5]Laminin抗体[MEC5]|Abcam中国|Anti-Laminin抗体[MEC5]
Laminin大鼠单克隆抗体[MEC5](ab2466)可与小鼠, 大鼠, 人样本反应并经IHC实验严格验证，Abcam Laminin抗体被2篇文献引用并得到1个独立的用户反馈。所有产品均提供质保服务，中国75%以上现货。
http://www.abcam.cn/laminin-antibody-mec5-ab2466.html?productWallTab=Questions
Pulmonary CellsPulmonary Cells
Am. J. Respir. Cell Mol. BioI. 1996. Here, the authors studied the expression of different laminin chains and their integrin receptors in fetal and adult lung by immunohistochemistry. Special attention was focused on the changes in the expression of these proteins during the development from the pseudoglandular (PG) and canalicular stages to adult lung and on the possible implications of the changes for the normal lung development. The most significant changes in the expression pattern were found during the development from the PO stage to the canalicular stage. Basement membranes (BM) of both the epithelial buds and the becoming bronchi showed reactivity for laminin α1, a3, and B3 chains at all stages. The a2 chain was expressed only in the epithelial buds at the PO stage, and could not be found in any epithelial structures at the canalicular stage. Similarly, at the PG stage the B2 chain was expressed in BMs of both epithelial buds and bronchi but disappeared from the bronchial BM before the ...
http://www.thescienceroom.com/laminin-lung-cell-articles
Effects of fibronectin and laminin on structural, mechanical and transport properties of 3D collageneous network | SpringerLinkEffects of fibronectin and laminin on structural, mechanical and transport properties of 3D collageneous network | SpringerLink
Recent studies, on cells cultured in 3D collagen gels, have shown that, beside from their well known biochemical role, fibronectin (FN) and laminin (LM) affect cell functions via a modification of mec
https://link.springer.com/article/10.1007%2Fs10856-006-0686-5
anti-Laminin gamma 1 antibody, Internal | GeneTexanti-Laminin gamma 1 antibody, Internal | GeneTex
Laminin gamma 1 antibody, Internal (laminin subunit gamma 1) for WB. Anti-Laminin gamma 1 pAb (GTX44865) is tested in Human samples. 100% Ab-Assurance.
http://www.genetex.com/LAMC1-antibody-Internal-GTX44865.html
anti-Laminin alpha 4 antibody [C3], C-term | GeneTexanti-Laminin alpha 4 antibody [C3], C-term | GeneTex
Laminin alpha 4 antibody [C3], C-term (laminin, alpha 4) for IHC-P. Anti-Laminin alpha 4 pAb (GTX106134) is tested in Mouse samples. 100% Ab-Assurance.
http://www.genetex.com/Laminin-alpha-4-antibody-C3-C-term-GTX106134.html
Laminin SIKVAV peptide induction of monocyte/macrophage prosta...Laminin SIKVAV peptide induction of monocyte/macrophage prosta...
Laminin SIKVAV peptide induction of monocyte/macrophage prostaglandin E2 and matrix metalloproteinases.: The laminin-derived synthetic peptide containing the SI
https://www.mysciencework.com/publication/show/laminin-sikvav-peptide-induction-monocyte-macrophage-prostaglandin-e2-matrix-metalloproteinases-73fb5480
Effects of oral administration of sodium nitrite on laminin expression in mice testicular interstitium: an immunohistochemical...Effects of oral administration of sodium nitrite on laminin expression in mice testicular interstitium: an immunohistochemical...
Effects of oral administration of sodium nitrite on laminin expression in mice testicular interstitium: an immunohistochemical study, Sara Amini, Mohammad Reza Nikravesh, Mehdi Jal
https://www.alliedacademies.org/articles/effects-of-oral-administration-of-sodium-nitrite-on-laminin-expression-in-mice-testicular-interstitium-an-immunohistochemical-stud-8781.html
Merosin-deficient congenital muscular dystrophy. Partial genetic correction in two mouse modelsMerosin-deficient congenital muscular dystrophy. Partial genetic correction in two mouse models
Humans and mice with deficiency of the alpha2 subunit of the basement membrane protein laminin-2/merosin suffer from merosin-deficient congenital muscular dystrophy (MCMD). We have expressed a human laminin alpha2 chain transgene under the regulation of a muscle-specific creatine kinase promoter in …
https://pubmed.ncbi.nlm.nih.gov/9710454/?dopt=Abstract
ORBi: Browsing ORBiORBi: Browsing ORBi
in Journal of the National Cancer Institute (1992), 84(15), 1161-9. BACKGROUND: Interactions between cells and the basement membrane glycoprotein laminin are altered during colon cancer progression. Colon carcinoma and normal mucosa cells express a variety of laminin ... [more ▼]. BACKGROUND: Interactions between cells and the basement membrane glycoprotein laminin are altered during colon cancer progression. Colon carcinoma and normal mucosa cells express a variety of laminin-binding proteins, including the 67-kd laminin receptor (67 LR) and a 31-kd human laminin-binding protein (HLBP31) homologous to the 31-kd human IgE-binding protein/galactoside-binding lectin. PURPOSE: To investigate whether various laminin-binding proteins are differentially expressed in human colon carcinoma, we studied messenger RNA (mRNA) levels of the 67 LR and HLBP31 in matched tumor and adjacent normal mucosa samples from a series of 21 patients. METHODS: Total cellular RNA from tumor and normal mucosa was isolated ...
http://orbi.ulg.ac.be/browse?type=author&value=van+den+Brule%2C+F.+A
Laminin assembles into separate basement membrane and fibrillar matrices in Schwann cells | Journal of Cell ScienceLaminin assembles into separate basement membrane and fibrillar matrices in Schwann cells | Journal of Cell Science
Schwann cell basement membranes do not appear to form in developing tissues until the stage of neuronal ensheathment, and it has been postulated that axonal contact triggers their assembly, in turn affecting cell polarization, ensheathment and myelination ( Clark and Bunge, 1989; Jaakkola et al., 1993). The data presented in this study suggest that Schwann cell basement membrane assembly is a process that first requires the development of cell surface competency for laminin binding, followed by the binding and assembly of laminin. We consider this ECM as a `nascent basement membrane (i.e. a laminin-based ECM with classical ultrastructural morphology of a basement membrane, but still lacking in type IV collagen). The nascent basement membrane is likely to be no more than one laminin molecular layer thick. Upon exposure of the cells to type IV collagen, this component is incorporated into the laminin matrix. In Schwann cells, the laminin binding/assembly step depends in part upon an interaction ...
http://jcs.biologists.org/content/115/5/1005
Comment on: Korpos et al. The Peri-islet Basement Membrane, a Barrier to Infiltrating Leukocytes in Type 1 Diabetes in Mouse...Comment on: Korpos et al. The Peri-islet Basement Membrane, a Barrier to Infiltrating Leukocytes in Type 1 Diabetes in Mouse...
Korpos et al. (1) claim that limited information exists on the nature of the ECM [extracellular matrix] of the pancreas and, in particular, on the composition of peri-islet capsule, and that they provide the the first comprehensive analysis of the [ECM] composition of peri-islet capsules.. We dispute the strength of these claims. In mice we originally reported in 2008 that a basement membrane (BM) exists around islets, and eight unique components were identified (type IV collagens α1, α2, laminin α2, β1, γ1, nidogen 1, nidogen 2, perlecan) as well as two composite components (laminin Engelbreth-Holm-Swarm, collagen IV) (2). It was also shown that type IV collagens α3, α4, α5, α6 were not present (2). Korpos et al. (1) were able to find a further three unique components (laminin α4, β2, agrin) and one composite component (laminin 322).. In 2008 in humans (3,4), at least four unique components (laminin α1, α5, β1, γ1) were identified and in their Supplementary Figure 1 staining ...
http://diabetes.diabetesjournals.org/content/62/8/e13.full?cited-by=yes&legid=diabetes
Rodent myoblast interactions with laminin require cell surface glycoconjugates but not laminin glycosyl groups<...
TY - JOUR. T1 - Rodent myoblast interactions with laminin require cell surface glycoconjugates but not laminin glycosyl groups. AU - Kostrominova, Tatiana. AU - Tanzer, M. L.. PY - 1995. Y1 - 1995. N2 - Laminin glycosyl groups are necessary for the spreading of murine melanoma cells which become attached to this glycoprotein. Laminin has been implicated in myogenesis but the potential role of its glycosyl groups in this process has not been examined. In this study we report the effects of the carbohydrate moieties of laminin on myoblast adhesion, spreading, and differentiation. Unglycosylated laminin from tunicamycin-treated cultures of a mouse cell line, M1536 B3, was used in the experiments. Glycosylated laminin from a murine tumor and from cultures of M1563 B3 cells served as controls. Cell binding experiments with C2C12 mouse myoblasts showed that the cells preferred a laminin-coated surface, compared to the uncoated plastic surface (nontissue culture wells). Myoblasts did not distinguish ...
https://indiana.pure.elsevier.com/en/publications/rodent-myoblast-interactions-with-laminin-require-cell-surface-gl
UFZ - Publikationsverzeichnis - Helmholtz-Zentrum für Umweltforschung UFZ
The disulfide bonding patterns in the N-terminal (LN) domains of the basement membrane protein laminin beta 1 have not been investigated so far. We report an in-depth mass spectrometric analysis using offline nano-high-performance liquid chromatography/matrix-assisted laser desorption/ionization time-of-flight/time-of-flight mass spectrometry (nano-HPLC/MALDI-TOF/TOF-MS) for determining the disulfide bond patterns in the LN-domain of recombinant mouse laminin beta 1 chain for the first time. Mass spectra were recorded and the putatively disulfide-linked peptides were subjected to LIFT-TOF/TOF-MS to confirm the disulfide bond. Screening the fragment ion mass spectra of disulfide-linked peptides for characteristic 66-amu patterns (34 u +32 u), arising from symmetric and asymmetric cleavage of disulfide bonds, facilitated their identification. Using various enzymes for proteolytic digestion of a recombinant laminin beta 1 chain N-terminal protein fragment, a linear bonding pattern of the eight ...
https://www.ufz.de/index.php?de=20939&pub_id=1084
Investigation of the role of the non-integrin laminin receptor in the pathogenesis of bacterial meningitis - Nottingham ePrintsInvestigation of the role of the non-integrin laminin receptor in the pathogenesis of bacterial meningitis - Nottingham ePrints
The human non-integrin laminin receptor (LamR) is a multifunctional protein which is localised to a number of sub-cellular locations. LamR is a component of the ribosome and has a number of intracellular functions; it also acts as an extracellular receptor for laminin, growth factors, pathogenic microorganisms, prion proteins, toxins and the anticarcinogen epigallocatechin gallate (ECGC). Although LamR is present in most cellular compartments, its overexpression in many types of cancer cells suggests a vital role for LamR in tumor-cell migration and invasion. There are two isoforms of laminin receptor: the monomeric 37-kDa laminin receptor precursor (37LRP) and the mature 67-kDa laminin receptor (67LR). Although the precise molecular nature of 67LR is unclear, accumulating evidence strongly suggest that 37LRP can undergo homo- and/or hetero-dimerization with Galectin-3 (Gal-3) to form mature 67LR. A recent study demonstrated that both homo- and heterodimer LamR forms are present on the cell ...
https://eprints.nottingham.ac.uk/31739/
In vivo embryonic expression of laminin and its involvement in cell shape change in the sea urchin Sphaerechinus granularis |...In vivo embryonic expression of laminin and its involvement in cell shape change in the sea urchin Sphaerechinus granularis |...
Laminin, a component of the embryonic sea urchin basal lamina, is recognized by monoclonal antibody BL1 (Mab BL1). Our results demonstrate that laminin is secreted into the blastcoel at the early blastula stage at a time when the blastomeres undergo a cell shape change and are organized into an epithelium. Laminin is present on the basal surfaces of ectodermal cells and is absent or reduced on migrating primary mesenchyme cells. Microinjection of a monoclonal antibody directed against laminin induces a morphological change in cell shape and a deformation of the embryonic epithelium. Investigation of selected stages of live embryos suggests that the distribution of laminin may be heterogeneous within the basal lamina during early development. The results implicate laminin as a mediator of cell shape change during early morphogenesis.. ...
http://dev.biologists.org/content/101/4/659
The 16th International Mouse Genome Conference (2002)The 16th International Mouse Genome Conference (2002)
Institutions: 1) University of Nottingham, 2) University of Vienna. The myodystrophy mouse is a spontaneous, autosomal recessive model of muscular dystrophy. We previously identified the molecular defect underlying the myodystrophy (Largemyd) mouse as a deletion in the Large gene, encoding a glycosyltransferase. This mutant is currently the only animal model for studying the role of glycosylation in the group of human muscle disorders due to mutated glycosyltransferases.Dystroglycan, an extensively modified glycoprotein, is a central component of the dystrophin-associated glycoprotein complex; this plays an important role in maintaining muscle membrane integrity. Furthermore, glycosylation of ?lpha?dystroglycan is necessary for its interaction with the extracellular matrix protein laminin. Western analyses of Largemyd proteins reveal hypo-glycosylation of alpha-dystroglycan and loss of laminin binding. We are currently using the glycan-binding specificities of lectins to investigate the overall ...
http://www.imgs.org/Archive/abstracts/2002abstracts/file249.shtml
ORBi: Browsing ORBiORBi: Browsing ORBi
in Invasion & Metastasis (1993), 13(1), 1-30. Interactions between cancer cells and laminin, a major component of basement membranes, play a critical role at several steps of the complex process of tumor invasion and metastasis. These interactions ... [more ▼]. Interactions between cancer cells and laminin, a major component of basement membranes, play a critical role at several steps of the complex process of tumor invasion and metastasis. These interactions are mediated through a large variety of cell surface proteins designated as laminin receptors and/or laminin-binding proteins. The growing number of identified laminin binding proteins and domains of this glycoprotein that are biologically active illustrate the complexity of cellular interactions with laminin. The 67-kD laminin receptor (67 LR) was the first protein identified in 1983 as a high-affinity laminin receptor, and its expression is dramatically increased in a large variety of cancer cells. The 67 LR is the subject of several ...
http://orbi.ulg.ac.be/browse?type=journal&rpp=20&value=Invasion+%26+Metastasis
Laminin and tenascin assembly and expression regulate HC11 mouse mammary cell differentiation | Journal of Cell ScienceLaminin and tenascin assembly and expression regulate HC11 mouse mammary cell differentiation | Journal of Cell Science
HC11 is a normal mouse mammary epithelial cell line that requires certain growth factors, such as EGF or bFGF, to respond optimally to lactogenic hormones and produce the differentiation marker beta-casein. Growth in insulin (Ins) or PDGF does not produce cells competent to respond to lactogenic hormones. Here we show that competency for differentiation is due at least in part to the modulation of extracellular matrix components. In particular we have studied laminin and tenascin. EGF alters endogenous laminin assembly. In addition, promotion of competency can be partially mimicked by plating HC11 cells on the E8 laminin fragment, which is able to induce lactogenic responsiveness in cells grown in the absence of EGF or bFGF. The production and assembly of tenascin is also dependent upon the growth conditions of the HC11 cells. EGF- or bFGF-grown competent cells produce tenascin but do not assemble it at the extracellular matrix as efficiently as Ins- or PDGF-grown, non-competent cells. This ...
http://jcs.biologists.org/content/107/4/1031
The activation dependent adhesion of macrophages to laminin involves cytoskeletal anchoring and phosphorylation of the alpha 6...The activation dependent adhesion of macrophages to laminin involves cytoskeletal anchoring and phosphorylation of the alpha 6...
Macrophages require activation with either PMA (Mercurio, A. M., and L. M. Shaw. 1988. J. Cell Biol. 107:1873-1880) or interferon-gamma (Shaw, L. M., and A. M. Mercurio. 1989. J. Exp. Med. 169:303-308) to adhere to a laminin substratum. In the present study, we identified an integrin laminin receptor on macrophages and characterized cellular changes that occur in response to PMA activation that facilitate laminin adhesion. A monoclonal antibody (GoH3) that recognizes the integrin alpha 6 subunit (Sonnenberg, A., H. Janssen, F. Hogervorst, J. Calafat, and J. Hilgers. 1987. J. Biol. Chem. 262:10376-10383) specifically inhibited adhesion to laminin-coated surfaces. This antibody precipitated an alpha 6 beta 1 heterodimer (Mr 130/110 kD) from 125I surface-labeled macrophages. The amount of radiolabeled receptor on the cell surface did not increase after PMA activation. Thus, the induction of laminin adhesion cannot be attributed to de novo or increased surface expression of alpha 6 beta 1. By ...
https://rupress.org/jcb/article/110/6/2167/21547/The-activation-dependent-adhesion-of-macrophages
Changes in the extracellular matrix components fibronectin and laminin during immune rejection of skeletal muscle<...
TY - JOUR. T1 - Changes in the extracellular matrix components fibronectin and laminin during immune rejection of skeletal muscle. AU - Gulati, A. K.. AU - Reddi, A Hari. AU - Zalewski, A. A.. PY - 1984. Y1 - 1984. N2 - Extracellular matrix is known to play an important role during development and maintenance of various tissues. In the present study, changes in two extracellular matrix glycoproteins, fibronec ?ptin and laminin, were investigated in skeletal muscle undergoing immune rejection. Purified antibodies against fibronectin and laminin were used to analyze the matrix by indirect immunofluorescence at various intervals after transplantation of extensor digitorum muscle in rats. Fibronectin and laminin were localized in the pericellular basement membrane zone of the normal myofibers; however, the cytoplasm was devoid of both glycoproteins. Transplanted muscle grafts underwent a process of degeneration and then an initial regeneration during the first 7 days. This regeneration effort ceased ...
https://ucdavis.pure.elsevier.com/en/publications/changes-in-the-extracellular-matrix-components-fibronectin-and-la
Laminin, alpha 4 | definition of laminin, alpha 4 by Medical dictionaryLaminin, alpha 4 | definition of laminin, alpha 4 by Medical dictionary
Looking for online definition of laminin, alpha 4 in the Medical Dictionary? laminin, alpha 4 explanation free. What is laminin, alpha 4? Meaning of laminin, alpha 4 medical term. What does laminin, alpha 4 mean?
https://medical-dictionary.thefreedictionary.com/laminin%2C+alpha+4
Laminin a2 Merosin deficiency - Muscular Dystrophy - Fitness VIPLaminin a2 Merosin deficiency - Muscular Dystrophy - Fitness VIP
In humans, mutations in the lama2 gene, which encodes laminin a2, cause merosin-deficient congenital muscular dystrophy 1A MDC1A , one subset of a broad
https://www.fitness-vip.com/muscular-dystrophy/laminin-a2-merosin-deficiency.html
T-plastin is essential for basement membrane assembly and epidermal morphogenesis | Science SignalingT-plastin is essential for basement membrane assembly and epidermal morphogenesis | Science Signaling
The establishment of epithelial architecture is a complex process involving cross-talk between cells and the basement membrane. Basement membrane assembly requires integrin activity but the role of the associated actomyosin cytoskeleton is poorly understood. Here, we identify the actin-bundling protein T-plastin (Pls3) as a regulator of basement membrane assembly and epidermal morphogenesis. In utero depletion of Pls3 transcripts in mouse embryos caused basement membrane and polarity defects in the epidermis but had little effect on cell adhesion and differentiation. Loss-of-function experiments demonstrated that the apicobasal polarity defects were secondary to the disruption of the basement membrane. However, the basement membrane itself was profoundly sensitive to subtle perturbations in the actin cytoskeleton. We further show that Pls3 localized to the cell cortex, where it was essential for the localization and activation of myosin II. Inhibition of myosin II motor activity disrupted ...
https://stke.sciencemag.org/content/10/481/eaal3154
Max Planck Society - eDoc ServerMax Planck Society - eDoc Server
Endothelial basement membranes constitute barriers to extravasating leukocytes during inflammation, a process where laminin isoforms define sites of leukocyte exit; however, how this occurs is poorly understood. In addition to a direct effect on leukocyte transmigration, we show that laminin 511 affects endothelial barrier function by stabilizing VE-cadherin at junctions and downregulating expression of CD99L2, correlating with reduced neutrophil extravasation. Binding of endothelial cells to laminin 511, but not laminin 411 or non-endothelial laminin 111, enhanced transendothelial cell electrical resistance (TEER) and inhibited neutrophil transmigration. Data suggest that endothelial adhesion to laminin 511 via beta1 and beta3 integrins mediates RhoA-induced VE-cadherin localization to cell-cell borders, and while CD99L2 downregulation requires integrin beta1, it is RhoA-independent. Our data demonstrate that molecular information provided by basement membrane laminin 511 affects leukocyte ...
http://edoc.mpg.de/display.epl?mode=doc&id=744101&col=77&grp=4088
Conjugation of LG domains of agrins and perlecan to polymerizing laminin-2 promotes acetylcholine receptor clustering - edocConjugation of LG domains of agrins and perlecan to polymerizing laminin-2 promotes acetylcholine receptor clustering - edoc
Neuromuscular junction (NMJ) assembly is characterized by the clustering and neuronal alignment of acetylcholine receptors (AChRs). In this study we have addressed post-synaptic contributions to assembly that may arise from the NMJ basement membrane with cultured myotubes. We show that the cell surface-binding LG domains of non-neural (muscle) agrin and perlecan promote AChR clustering in the presence of laminin-2. This type of AChR clustering occurs with a several hour lag, requires muscle-specific kinase (MuSK), and is accompanied by tyrosine phosphorylation of MuSK and betaAChR. It also requires conjugation of the agrin or perlecan to laminin together with laminin polymerization. Furthermore, AChR clustering can be mimicked with antibody binding to non-neural agrin, supporting a mechanism of ligand aggregation. Neural agrin, in addition to its unique ability to cluster AChRs through its B/z sequence insert, also exhibits laminin-dependent AChR clustering, the latter enhancing and stabilizing ...
http://edoc.unibas.ch/2753/
EFFECTS OF PUERARIN ON RENAL FUNCTION AND EXPRESSIONS OF MMP-3 AND TIMP-1 IN THE KIDNEY OF DIABETIC RATS--《Natural Product...EFFECTS OF PUERARIN ON RENAL FUNCTION AND EXPRESSIONS OF MMP-3 AND TIMP-1 IN THE KIDNEY OF DIABETIC RATS--《Natural Product...
Objective:To investigate the effects of puerarin on renal structure?function and expressions of MMP-3 and TIMP-1 in the kidney of diabetic rats.Methods:Uninephrectomized STZ-induced male wister rats were used.Puerarin was delivered daily by the intraperitoneal injection from the third day of induction to diabetes for 16 weeks.Using in situ hybridization and immunohistochemistry to detect TIMP-1mRNA expression and MMP-3?TIMP-1?collagen Ⅳ and Laminin expressions in diabetic kidneys with image analysis system;Flow cytometry was used to detect the expressions of MMP-3?TIMP-1.Results:BUN?Ccr and Upro in diabetic rats were significant higher than that in control group.Compared with those in kidneys of control group,diabetic kidneys showed increased expressions of both MMP-3?TIMP-1 proteins and excessive deposition of glomeruli extracellular matrix(P0.05).The blood glucose and the level of expressions of MMP-3?TIMP-1?collagen Ⅳ and Laminin were reduced by puerarin treatment,renal function was improved
http://en.cnki.com.cn/Article_en/CJFDTOTAL-TRCW200306014.htm
Anti-RPSA / Laminin Receptor Antibody | Mouse anti-Human ELISA,WB | LSBioAnti-RPSA / Laminin Receptor Antibody | Mouse anti-Human ELISA,WB | LSBio
Laminin Receptor antibody LS-C197601 is an unconjugated mouse monoclonal antibody to human Laminin Receptor (RPSA). Validated for ELISA and WB.
https://www.lsbio.com/antibodies/rpsa-antibody-laminin-receptor-antibody-clone-8g4-elisa-wb-western-ls-c197601/205657
Serum laminin and type IV collagen are accurate markers of histologically severe alcoholic hepatitis in patients with cirrhosis...Serum laminin and type IV collagen are accurate markers of histologically severe alcoholic hepatitis in patients with cirrhosis...
PubMed comprises more than 30 million citations for biomedical literature from MEDLINE, life science journals, and online books. Citations may include links to full-text content from PubMed Central and publisher web sites.
https://www.ncbi.nlm.nih.gov/pubmed/10735610?dopt=Abstract
Basement membrane protein influences the connection of blood vessel cells - Healthcare TimesBasement membrane protein influences the connection of blood vessel cells - Healthcare Times
In previous studies, researchers at the Cells-in-Motion Cluster of Excellence had discovered that specific proteins in the basement membrane - so-called laminins - influence the migration of the immune cells. For example, they determine the place at which immune cells cross the basement membrane and enter into the tissue. Researchers have now discovered that these components of the basement membrane also affect the inner endothelial cell layer.. A certain laminin - laminin 511 - influences how tightly endothelial cells are connected to each other, explains biochemist Prof. Lydia Sorokin. This is because laminin affects specific molecules that interconnect endothelial cells. For one thing, laminin stabilizes the VE-cadherin molecule at contact sites between adjacent endothelial cells, forming a sort of Velcro fastener between them. In addition, laminin 511 reduces the quantity of a molecule known as CD99L2.. This is found on both the endothelial cells and on the immune cells and permits the ...
http://healthcaretimes.org/2017/02/03/basement-membrane-protein-influences-the-connection-of-blood-vessel-cells/
Role of the Basement Membrane Proteins Nidogen-1 and -2 and Laminin-Nidogen Interaction in Physiology and Pathology of Skin -...Role of the Basement Membrane Proteins Nidogen-1 and -2 and Laminin-Nidogen Interaction in Physiology and Pathology of Skin -...
Mokkapati, Sharada (2007) Role of the Basement Membrane Proteins Nidogen-1 and -2 and Laminin-Nidogen Interaction in Physiology and Pathology of Skin. [Thesis Abstract]. ...
http://kups.ub.uni-koeln.de/2222/
Geltrex™ hESC-Qualified, Ready-To-Use, Reduced Growth Factor Basement Membrane MatrixGeltrex™ hESC-Qualified, Ready-To-Use, Reduced Growth Factor Basement Membrane Matrix
Geltrex hESC-Qualified, Ready-To-Use, Reduced Growth Factor Basement Membrane Matrix is a soluble form of basement membrane extracted from murine Engelbreth-Holm-Swarm (EHS) tumors. This pre-diluted version of the matrix can be taken directly from the refrigerator and dispensed into cell culture pla
https://www.thermofisher.com/order/catalog/product/A1569601?ICID=search-product
Basement membrane protein nidogen-1 shapes hippocampal synaptic plasticity and excitability (2010) | SFB 829Basement membrane protein nidogen-1 shapes hippocampal synaptic plasticity and excitability (2010) | SFB 829
The basement membrane (BM) is a specialized form of extracellular matrix (ECM) underlying epithelia and endothelia and surrounding many types of mesenchymal cells. Nidogen, along with collagen IV and laminin, is a major component of BMs. Although certain ECM proteins such as laminin or reelin influence neuronal function via interactions with cell-surface receptors such as integrins, behavioral neurological impairments due to deficits of BM components have been recognized only recently. Here, alterations in neuronal network function underlying these behavioral changes are revealed. Using nidogen-1 knockout mice, with or without additional heterozygous nidogen-2 knockout (NID1(-/-)/NID2(+/+) or NID1(-/-)/NID2(+/-)), we demonstrate that nidogen is essential for normal neuronal network excitability and plasticity. In nidogen-1 knockouts, seizurelike behavior occurs, and epileptiform spiking was seen in hippocampal in vivo EEG recordings. In vitro, hippocampal field potential recordings revealed that ...
http://sfb829.uni-koeln.de/press-releases-special-issues-awardsbis-2015-b1-nischtbasement-membrane-protein-nidogen-1-shapes-hippocampal-synaptic-plasticity-and-excitability-2010/
Corning® Matrigel® Basement Membrane Matrix, *LDEV-Free, 50mL from Corning Life Sciences | SelectScienceCorning® Matrigel® Basement Membrane Matrix, *LDEV-Free, 50mL from Corning Life Sciences | SelectScience
Read independent reviews on Corning® Matrigel® Basement Membrane Matrix, *LDEV-Free, 50mL from Corning Life Sciences on SelectScience
https://www.selectscience.net/products/corning-matrigel-basement-membrane-matrix,-ldev-free,-50ml/?prodID=199012
Stem Cell Research Products from Takara ClontechStem Cell Research Products from Takara Clontech
Stem cell culture is accompanied by extra challenges, and it is critical to maintain genetic stability, ensure stemness, and prevent spontaneous differentiation. Cellartis media and supplements are optimized for derivation, maintenance, and expansion of pluripotent cells.. The Cellartis DEF-CS system is a complete culture system for robust expansion of human iPS cells in a feeder-free and defined environment. A xeno-free version is also available.. iMatrix-511 is a xeno-free recombinant laminin fragment for ES or iPS cell culture substrate; iMatrix-511 provides greater adhesive properties than full-length laminin, vitronectin, or reagents containing an undefined mixture of basement membrane.. ...
http://www.clontech.com/FR/Products/Stem_Cell_Research/Resources/Stem_Cell_Product_Overview?utm_source=cellartis.com&utm_medium=401&utm_campaign=web/index.php
Integrin alpha6beta1 Summary Report | CureHunterIntegrin alpha6beta1 Summary Report | CureHunter
Integrin alpha6beta1: A cell surface receptor mediating cell adhesion to the EXTRACELLULAR MATRIX and to other cells via binding to LAMININ. It is involved in cell migration, embryonic development, leukocyte activation and tumor cell invasiveness. Integrin alpha6beta1 is the major laminin receptor on PLATELETS; LEUKOCYTES; and many EPITHELIAL CELLS, and ligand binding may activate a number of signal transduction pathways. Alternative splicing of the cytoplasmic domain of the alpha6 subunit (INTEGRIN ALPHA6) results in the formation of A and B isoforms of the heterodimer, which are expressed in a tissue-specific manner.
http://www.curehunter.com/public/keywordSummaryD039121-I-c-IIa--Platelet-Glycoprotein.do
Laminin, Noahs Ark, and Why I Dont Like Meme Christianity | Brandon J. AdamsLaminin, Noahs Ark, and Why I Dont Like Meme Christianity | Brandon J. Adams
Nothing but the greatest affection towards my fellow believers is intended in this post. Theres a popular idea in circulation, commonly known from speaker Louie Giglio, that likes to speak of the Cross-resembling shape of the protein laminin, in conjunction with Colossians 1:17 (He is before all things, and in him all things hold together),…
https://brandonjadams.com/2019/03/28/laminin-ark-meme-christianity/?like_comment=6501&_wpnonce=df2e27512e&replytocom=6486
Laminin, Noahs Ark, and Why I Dont Like Meme Christianity | Brandon J. AdamsLaminin, Noahs Ark, and Why I Dont Like Meme Christianity | Brandon J. Adams
Nothing but the greatest affection towards my fellow believers is intended in this post. Theres a popular idea in circulation, commonly known from speaker Louie Giglio, that likes to speak of the Cross-resembling shape of the protein laminin, in conjunction with Colossians 1:17 (He is before all things, and in him all things hold together),…
https://brandonjadams.com/2019/03/28/laminin-ark-meme-christianity/?like_comment=6504&_wpnonce=24e3525b7a
DeCS Ingl s+escopo
A cell surface receptor mediating cell adhesion to the EXTRACELLULAR MATRIX and to other cells via binding to LAMININ. It is involved in cell migration, embryonic development, leukocyte activation and tumor cell invasiveness. Integrin alpha6beta1 is the major laminin receptor on PLATELETS; LEUKOCYTES; and many EPITHELIAL CELLS, and ligand binding may activate a number of signal transduction pathways. Alternative splicing of the cytoplasmic domain of the alpha6 subunit (INTEGRIN ALPHA6) results in the formation of A and B isoforms of the heterodimer, which are expressed in a tissue-specific manner. . ...
https://trigramas.bireme.br/cgi-bin/mx/[email protected]?collection=DeCSxi&lang=i&minsim=0.30&maxrel=10&text=CD49d-CD29
DeCS Ingl s+escopo
A cell surface receptor mediating cell adhesion to the EXTRACELLULAR MATRIX and to other cells via binding to LAMININ. It is involved in cell migration, embryonic development, leukocyte activation and tumor cell invasiveness. Integrin alpha6beta1 is the major laminin receptor on PLATELETS; LEUKOCYTES; and many EPITHELIAL CELLS, and ligand binding may activate a number of signal transduction pathways. Alternative splicing of the cytoplasmic domain of the alpha6 subunit (INTEGRIN ALPHA6) results in the formation of A and B isoforms of the heterodimer, which are expressed in a tissue-specific manner. . ...
https://trigramas.bireme.br/cgi-bin/mx/[email protected]?collection=DeCSxi&lang=i&minsim=0.30&maxrel=10&text=Integrin%20alpha
CD239 (BCAM) Antibody, anti-human, REAfinity™ - Recombinant antibodies - MACS Antibodies - Products - Miltenyi Biotec -...
Clone REA276 recognizes the CD239 antigen, a type I membrane glycoprotein also known as basal cell adhesion molecule (BCAM) or Lutheran blood group glycoprotein (LU), which are two alternatively spliced variants of a single immunoglobulin superfamily protein that differ in the length of their cytoplasmic tails. Mouse and human CD239 is highly conserved at the amino acid sequence level. CD239 has a wide tissue distribution and is closely associated with the basal layer of cells in epithelia and the endothelium of blood vessel walls. The expression of CD239 is higher in fetal versus adult tissues, and is upregulated following malignant transformation in some cell types. CD239 is the receptor for laminin alpha-5, an extracellular matrix protein involved in cell differentiation, adhesion, migration, and proliferation. It may plays a role in epithelial cell cancer and in vaso-occlusion of red blood cells in sickle cell disease. Additional information: Clone REA276 displays negligible binding to Fc receptors.
https://www.miltenyibiotec.com/LU-en/products/cd239-bcam-antibody-anti-human-reafinity-rea276.html
CD239 (BCAM) Antibody, anti-human, REAfinity™ | Recombinant antibodies | MACS Antibodies | Products | Miltenyi Biotec | 中国
Clone REA276 recognizes the CD239 antigen, a type I membrane glycoprotein also known as basal cell adhesion molecule (BCAM) or Lutheran blood group glycoprotein (LU), which are two alternatively spliced variants of a single immunoglobulin superfamily protein that differ in the length of their cytoplasmic tails. Mouse and human CD239 is highly conserved at the amino acid sequence level. CD239 has a wide tissue distribution and is closely associated with the basal layer of cells in epithelia and the endothelium of blood vessel walls. The expression of CD239 is higher in fetal versus adult tissues, and is upregulated following malignant transformation in some cell types. CD239 is the receptor for laminin alpha-5, an extracellular matrix protein involved in cell differentiation, adhesion, migration, and proliferation. It may plays a role in epithelial cell cancer and in vaso-occlusion of red blood cells in sickle cell disease.Additional information: Clone REA276 displays negligible binding to Fc receptors.
https://www.miltenyibiotec.com/CN-en/products/cd239-bcam-antibody-anti-human-reafinity-rea276.html
Effects of Maternal Nicotine Exposure on Laminin Αlpha 5 Expression Change of the Basal Membrane in the Distal Convoluted and...Effects of Maternal Nicotine Exposure on Laminin Αlpha 5 Expression Change of the Basal Membrane in the Distal Convoluted and...
Background and Objective: It is obviously approved that Fibronectin and Laminin α-5 (LAMA5) expression will change, in some organs of fetuses that their mothers were smoking during pregnancy period. For example, the expression of mentioned proteins in newborns decreases in parenchyma of their lungs. In present study, we evaluated the effects ...
http://journal.rums.ac.ir/browse.php?a_id=2180&slc_lang=en
The vascular basement membrane as soil in brain metastasis. - Oxford Neuroscience
Brain-specific homing and direct interactions with the neural substance are prominent hypotheses for brain metastasis formation and a modern manifestation of Pagets seed and soil concept. However, there is little direct evidence for this neurotropic growth in vivo. In contrast, many experimental studies have anecdotally noted the propensity of metastatic cells to grow along the exterior of pre-existing vessels of the CNS, a process termed vascular cooption. These observations suggest the soil for malignant cells in the CNS may well be vascular, rather than neuronal. We used in vivo experimental models of brain metastasis and analysis of human clinical specimens to test this hypothesis. Indeed, over 95% of early micrometastases examined demonstrated vascular cooption with little evidence for isolated neurotropic growth. This vessel interaction was adhesive in nature implicating the vascular basement membrane (VBM) as the active substrate for tumor cell growth in the brain. Accordingly, VBM
https://www.neuroscience.ox.ac.uk/publications/223355
Corning™ Matrigel™ Membrane Matrix LDEV-Free; 5mL Corning™ Matrigel™ Membrane MatrixCorning™ Matrigel™ Membrane Matrix LDEV-Free; 5mL Corning™ Matrigel™ Membrane Matrix
Corning™ Matrigel™ Membrane Matrix LDEV-Free; 5mL Corning™ Matrigel™ Membrane Matrix Dissociation Reagents
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Science meets God | Lisa Christiansens Wealth Of WisdomScience meets God | Lisa Christiansens Wealth Of Wisdom
Science Meets God Many of you are aware of my background in Exercise and Nutritional Sciences, here is where Science meets God. Laminins are major proteins in the basal lamina (one of the layers of the basement membrane), a protein network foundation for most cells and organs. The laminins are an important and biologically active…
https://lisa-christiansen.com/2012/02/10/science-meets-god/
The CROSS of Laminin ... The Protein Molecule of the body..linked to the BIBLE ..The CROSS of Laminin ... The Protein Molecule of the body..linked to the BIBLE ..
Business Owner Linda Morris published a press release titled: The CROSS of Laminin ... The Protein Molecule of the body..linked to the BIBLE ..
http://www.ibosocial.com/Linny13/pressrelease.aspx?prid=73584
Does Laminin hold our body together ... and does Avian Egg Powdered Extract support it ?Does Laminin hold our body together ... and does Avian Egg Powdered Extract support it ?
Business Owner Linda Morris published a press release titled: Does Laminin hold our body together ... and does Avian Egg Powdered Extract support it ?
http://www.ibosocial.com/Linny13/pressrelease.aspx?prid=74037
BD BioCoat Poly-D-Lysine/Laminin 96-well Microplates from BD Biosciences Discovery Labware | SelectScienceBD BioCoat Poly-D-Lysine/Laminin 96-well Microplates from BD Biosciences Discovery Labware | SelectScience
Read independent reviews on BD BioCoat Poly-D-Lysine/Laminin 96-well Microplates from BD Biosciences Discovery Labware on SelectScience
http://www.selectscience.net/products/bd-biocoat-poly-d-lysine+laminin-96-well-microplates/?prodID=107958
LamininLaminin
The laminin N-terminal domain is found in all laminin and netrin subunits except laminin alpha 3A, alpha 4 and gamma 2. Laminin ... Laminin domain II: all laminin alpha chains (LAMA1, LAMA2, LAMA3, LAMA4, LAMA5) Laminin B (domain IV): all laminin alpha chains ... LAMC3 Laminins were previously numbered as they were discovered, i.e. laminin-1, laminin-2, laminin-3, etc., but the ... laminin-211, etc.). In addition, many laminins had common names before either laminin nomenclature was in place. Laminins form ...
https://en.wikipedia.org/wiki/Laminin
Laminin databaseLaminin database
The Laminin database is a database of non-collagenous extracellular matrix proteins. Laminin Golbert, Daiane C F; Linhares- ... "Laminin database: a tool to retrieve high-throughput and curated data for studies on laminins". Nucleic Acids Res. England. 39 ... "Laminin-database v.2.0: an update on laminins in health and neuromuscular disorders". Nucleic Acids Research. 42 (D1): D426- ... Laminins, All stub articles, Biological database stubs). ...
https://en.wikipedia.org/wiki/Laminin_database
Laminin 111Laminin 111
Laminin-111 (also "laminin-1") is a protein of the type known as laminin isoforms. It was among the first of the laminin ... ECM laminin-111 levels in a normal breast cell are significantly higher than laminin-111 levels in tissues of cancerous breast ... How laminin-111 and nuclear actin communicate is one of these factors. Laminin-111 could be the physiological regulator of ... Adding laminin-111 to breast epithelial cells leads to quiescence by altering nuclear actin. High levels of laminin-111 deplete ...
https://en.wikipedia.org/wiki/Laminin_111
Laminin, beta 2Laminin, beta 2
... what was formerly Laminin 1 is now Laminin 111, and what was formerly Laminin 5 is now Laminin 332. The biological functions of ... Laminin subunit beta-2 is a protein that in humans is encoded by the LAMB2 gene. Laminins, a family of extracellular matrix ... Laminins are composed of 3 non identical chains: laminin alpha, beta and gamma (formerly A, B1, and B2, respectively) and they ... "Entrez Gene: LAMB2 laminin, beta 2 (laminin S)". Aumailley M, Bruckner-Tuderman L, Carter WG, Deutzmann R, Edgar D, Ekblom P, ...
https://en.wikipedia.org/wiki/Laminin,_beta_2
Laminin, alpha 5Laminin, alpha 5
Utani A, Nomizu M, Yamada Y (Jan 1997). "Fibulin-2 binds to the short arms of laminin-5 and laminin-1 via conserved amino acid ... Kikkawa Y, Sanzen N, Fujiwara H, Sonnenberg A, Sekiguchi K (Mar 2000). "Integrin binding specificity of laminin-10/11: laminin- ... "The short arm of the laminin gamma2 chain plays a pivotal role in the incorporation of laminin 5 into the extracellular matrix ... "Presence of laminin alpha5 chain and lack of laminin alpha1 chain during human muscle development and in muscular dystrophies ...
https://en.wikipedia.org/wiki/Laminin,_alpha_5
Laminin, beta 1Laminin, beta 1
Laminin subunit beta-1 is a protein that in humans is encoded by the LAMB1 gene. Laminins, a family of extracellular matrix ... Laminins are composed of 3 non identical chains: laminin alpha, beta and gamma (formerly A, B1, and B2, respectively) and they ... Laminin, beta 1 is expressed in most tissues that produce basement membranes, and is one of the 3 chains constituting laminin 1 ... 2000). "Integrin binding specificity of laminin-10/11: laminin-10/11 are recognized by alpha 3 beta 1, alpha 6 beta 1 and alpha ...
https://en.wikipedia.org/wiki/Laminin,_beta_1
Laminin, alpha 3Laminin, alpha 3
Laminin subunit alpha-3 is a protein that in humans is encoded by the LAMA3 gene. Laminins are basement membrane components ... Fujiwara H, Kikkawa Y, Sanzen N, Sekiguchi K (2001). "Purification and characterization of human laminin-8. Laminin-8 ... "Entrez Gene: LAMA3 laminin, alpha 3". McLean WH, Irvine AD, Hamill KJ, Whittock NV, Coleman-Campbell CM, Mellerio JE, Ashton GS ... Laminin 5 is thought to be involved in cell adhesion, signal transduction and differentiation of keratinocytes. Mutations in ...
https://en.wikipedia.org/wiki/Laminin,_alpha_3
Laminin, alpha 4Laminin, alpha 4
Laminin subunit alpha-4 is a protein that in humans is encoded by the LAMA4 gene. Laminins, a family of extracellular matrix ... Laminins are composed of 3 non identical chains: laminin alpha, beta and gamma (formerly A, B1, and B2, respectively) and they ... "Entrez Gene: LAMA4 laminin, alpha 4". Ljubimova JY, Fujita M, Khazenzon NM, Ljubimov AV, Black KL (2006). "Changes in laminin ... "The complete cDNA sequence of laminin alpha 4 and its relationship to the other human laminin alpha chains". European Journal ...
https://en.wikipedia.org/wiki/Laminin,_alpha_4
Laminin, beta 3Laminin, beta 3
Laminin subunit beta-3 is a protein that in humans is encoded by the LAMB3 gene. LAMB3 encodes the beta 3 subunit of laminin. ... "The complete primary structure for a novel laminin chain, the laminin B1k chain". J. Biol. Chem. 269 (15): 11073-80. doi: ... Laminin is composed of three subunits (alpha, beta, and gamma), and refers to a family of basement membrane proteins. For ... 1995). "Altered laminin 5 expression due to mutations in the gene encoding the beta 3 chain (LAMB3) in generalized atrophic ...
https://en.wikipedia.org/wiki/Laminin,_beta_3
Laminin subunit alpha-1Laminin subunit alpha-1
... is a protein that in humans is encoded by the LAMA1 gene. Laminin, alpha 1 has been shown to interact ... Utani A, Nomizu M, Yamada Y (1997). "Fibulin-2 binds to the short arms of laminin-5 and laminin-1 via conserved amino acid ... "Fibulin-2 binds to the short arms of laminin-5 and laminin-1 via conserved amino acid sequences". J. Biol. Chem. 272 (5): 2814- ... 1989). "Human laminin: cloning and sequence analysis of cDNAs encoding A, B1 and B2 chains, and expression of the corresponding ...
https://en.wikipedia.org/wiki/Laminin_subunit_alpha-1
Laminin subunit gamma-1Laminin subunit gamma-1
... is a protein that in humans is encoded by the LAMC1 gene. Laminins, a family of extracellular matrix ... Laminins are composed of 3 non identical chains: laminin alpha, beta and gamma (formerly A, B1, and B2, respectively) and they ... "Entrez Gene: LAMC1 laminin, gamma 1 (formerly LAMB2)". Ljubimova JY, Fujita M, Khazenzon NM, et al. (2006). "Changes in laminin ... 1991). "Structure of the human laminin B2 chain gene reveals extensive divergence from the laminin B1 chain gene". J. Biol. ...
https://en.wikipedia.org/wiki/Laminin_subunit_gamma-1
Laminin subunit alpha-2Laminin subunit alpha-2
... is a protein that in humans is encoded by the LAMA2 gene. Laminin, an extracellular matrix protein, is ... This gene encodes the alpha 2 chain, which constitutes one of the subunits of laminin 2 (merosin) and laminin 4 (s-merosin). ... 2000). "A novel laminin alpha2 isoform in severe laminin alpha2 deficient congenital muscular dystrophy". Neurology. 55 (8): ... 1999). "Characterization and Expression of the Laminin γ3 Chain: A Novel, Non-Basement Membrane-associated, Laminin Chain". J. ...
https://en.wikipedia.org/wiki/Laminin_subunit_alpha-2
Laminin subunit gamma-2Laminin subunit gamma-2
... is a protein that in humans is encoded by the LAMC2 gene. Laminins, a family of extracellular matrix ... Laminins are composed of 3 non identical chains: laminin alpha, beta and gamma (formerly A, B1, and B2, respectively) and they ... Each laminin chain is a multidomain protein encoded by a distinct gene. Several isoforms of each chain have been described. ... "Entrez Gene: LAMC2 laminin, gamma 2". Baudoin C, Miquel C, Gagnoux-Palacios L, et al. (1995). "A novel homozygous nonsense ...
https://en.wikipedia.org/wiki/Laminin_subunit_gamma-2
Laminin subunit gamma-3Laminin subunit gamma-3
... also known as LAMC3 is a protein that in humans is encoded by the LAMC3 gene. Laminins, a family of ... Laminins are composed of 3 non identical chains: laminin alpha, beta and gamma (formerly A, B1, and B2, respectively) and they ... 1998). "Human colonic cancer cells synthesize and adhere to laminin-5. Their adhesion to laminin-5 involves multiple receptors ... The distribution of gamma 3-containing laminins along ciliated epithelial surfaces suggests that the apical laminins are ...
https://en.wikipedia.org/wiki/Laminin_subunit_gamma-3
Aleph (pianist)Aleph (pianist)
"Dbayeh International Festival". LAMININ EVENTS. Retrieved August 29, 2019. "Ticketing BoxOffice for Lebanon, Saudi Arabia and ...
https://en.wikipedia.org/wiki/Aleph_(pianist)
Reicherts membraneReichert's membrane
The synthesis of laminin 111 in the embryo contributes to the formation of Reichert's membrane. Reichert's membrane functions ... It is composed of an extracellular matrix that includes laminin, type IV collagen, and nidogen, and is secreted by embryonic ... Miner, JH; Yurchenco, PD (2004). "Laminin functions in tissue morphogenesis". Annual Review of Cell and Developmental Biology. ...
https://en.wikipedia.org/wiki/Reichert’s_membrane
Karl TryggvasonKarl Tryggvason
His group has produced most laminins as recombinant human proteins and recently the group has shown that different laminin ... In 2008 he was the co-founder of BioLamina AB, a Swedish biotechnology company that produces laminins for cell therapy and ... 2005). "A simplified laminin nomenclature". Matrix Biology. 24 (5): 326-332. doi:10.1016/j.matbio.2005.05.006. PMID 15979864. ... His group has developed fully human and chemically defined laminin-based methods for generation of stem cell derived ...
https://en.wikipedia.org/wiki/Karl_Tryggvason
Lymph sacsLymph sacs
These markers include CD34 and Laminin. This experiment proves that Prox1 is necessary for lymphatic cells to specify. It is ...
https://en.wikipedia.org/wiki/Lymph_sacs
Diabetic cardiomyopathyDiabetic cardiomyopathy
July 1990). "Laminin alterations after in vitro nonenzymatic glycosylation". Diabetes. 39 (7): 807-14. doi:10.2337/diabetes. ...
https://en.wikipedia.org/wiki/Diabetic_cardiomyopathy
SarcolemmaSarcolemma
The basement membrane contains numerous thin collagen fibrils and specialized proteins such as laminin that provide a scaffold ... September 1990). "Distribution and isolation of four laminin variants; tissue restricted distribution of heterotrimers ...
https://en.wikipedia.org/wiki/Sarcolemma
NetrinNetrin
Structurally, netrin resembles the extracellular matrix protein laminin. Netrins are chemotropic; a growing axon will either ... which has several domains which are homologous with laminin at the amino terminal end. The C-terminal domain is where most of ...
https://en.wikipedia.org/wiki/Netrin
NidogenNidogen
Mayer U, Kohfeldt E, Timpl R (October 1998). "Structural and genetic analysis of laminin-nidogen interaction". Annals of the ... retaining their ability to bind laminin. In nematodes, nidogen-1 is necessary for axon guidance, but not for basement membrane ... "Analysis of nidogen-1/laminin γ1 interaction by cross-linking, mass spectrometry, and computational modeling reveals multiple ...
https://en.wikipedia.org/wiki/Nidogen
Glypican 2Glypican 2
Cerebroglycan has especially high affinity for laminin-1. GPC2 has been suggested as a therapeutic target in neuroblastoma. ...
https://en.wikipedia.org/wiki/Glypican_2
NeuroregenerationNeuroregeneration
Growth factors are not expressed or re-expressed; for instance, the extracellular matrix is lacking laminins. Glial scars ... ISBN 978-0-8385-7701-1. Stabenfeldt SE, García AJ, LaPlaca MC (June 2006). "Thermoreversible laminin-functionalized hydrogel ...
https://en.wikipedia.org/wiki/Neuroregeneration
HemidesmosomeHemidesmosome
Integrin α6β4 operates as a laminin-332 receptor. Integrin α6β4 is composed to two α and β subunit dimers. The larger β4 ... The α6 subunit binds to extracellular BP180, CD151 and laminin-322. When integrin α6β4 binds to Plectin 1a and BPAG1, it ... This is caused by mutations in integrin α6β4, laminin 322 and BPAG2. In dystrophic epidermolysis bullosa, the layers of the ... integrin β4 subunit in the cytoplasm and integrin α6 and laminin-332 in the extracellular space. CD151, a protein of the ...
https://en.wikipedia.org/wiki/Hemidesmosome
Nerve guidance conduitNerve guidance conduit
For patterns containing 0.5 mg/ml laminin, a higher proportion of neurites grew on the microdispensed lines than between the ... Also, the HA hydrogels modified with laminin were able to promote neurite extension. These results support HA gels as a ... The ECM components investigated were laminin-1 and fibronectin, which are natural ECM components, and ProNectin F plus (Pro-F) ... HA hydrogels that were either unmodified or modified with laminin were implanted into an adult central nervous system lesion ...
https://en.wikipedia.org/wiki/Nerve_guidance_conduit
OmigapilOmigapil
The majority of CMD cases result from a genetic mutation in laminin-α2, a subunit of the laminin-211 protein, which serves as ... The mouse model of laminin-α2-deficient congenital muscular dystrophy (MDC1A) was found to positively respond to omigapil with ... Colognato H, Yurchenco PD (June 2000). "Form and function: the laminin family of heterotrimers". Dev. Dyn. 218 (2): 213-34. doi ... laminin alpha 2) deficiency" (PDF). EMA/COMP/204694/2008 Rev.1. Muscular Dystrophy Campaign. "New collaboration to support ...
https://en.wikipedia.org/wiki/Omigapil
Ribosomal protein SARibosomal protein SA
RPSA was initially identified as a laminin binding protein. Both recombinant N-domain and C-domain of RPSA bind laminin in ... It also acts as a cell surface receptor, in particular for laminin, and is involved in several pathogenic processes. Laminins, ... "Altered levels of laminin receptor mRNA in various human carcinoma cells that have different abilities to bind laminin". Proc. ... 67 kDa Laminin Receptor; 67LR; 37/67 kDa Laminin Receptor; LRP/LR; LBP/p40; and p40 ribosome-associated protein. Ribosomal ...
https://en.wikipedia.org/wiki/Ribosomal_protein_SA
GLB1GLB1
Hinek A (1997). "Biological roles of the non-integrin elastin/laminin receptor". Biological Chemistry. 377 (7-8): 471-80. doi: ... "The 67-kDa enzymatically inactive alternatively spliced variant of beta-galactosidase is identical to the elastin/laminin- ...
https://en.wikipedia.org/wiki/GLB1
GAS6GAS6
Receptor tyrosine kinase activation by laminin G-like domains". J. Biol. Chem. 277 (46): 44164-70. doi:10.1074/jbc.M207340200. ...
https://en.wikipedia.org/wiki/GAS6
LAMC3 laminin subunit gamma 3 [Homo sapiens (human)] - Gene - NCBILAMC3 laminin subunit gamma 3 [Homo sapiens (human)] - Gene - NCBI
laminin subunit gamma-3. Names. laminin, gamma 3. laminin-12 subunit gamma. laminin-14 subunit gamma. laminin-15 subunit gamma ... Laminin_B; Laminin B (Domain IV). pfam00053. Location:917 → 965. Laminin_EGF; Laminin EGF domain. pfam00055. Location:44 → 269 ... Laminin_B; Laminin B (Domain IV). pfam00053. Location:917 → 965. Laminin_EGF; Laminin EGF domain. pfam00055. Location:44 → 269 ... Laminin_B; Laminin B (Domain IV). pfam00053. Location:917 → 965. Laminin_EGF; Laminin EGF domain. pfam00055. Location:44 → 269 ...
https://www.ncbi.nlm.nih.gov/gene/10319
Laminin German Glass Coverslips 24mm #1Laminin German Glass Coverslips 24mm #1
... Coated with natural mouse laminin on top of poly-d-lysine layer. Sterilized by ...
https://www.emsdiasum.com/laminin-german-glass-coverslips-24mm-1-2
Corning BioCoat Laminin Rectangular Canted Neck Cell Culture Flask w/Plug | Fisher ScientificCorning BioCoat Laminin Rectangular Canted Neck Cell Culture Flask w/Plug | Fisher Scientific
Corning™ BioCoat™ Laminin Rectangular Canted Neck Cell Culture Flask w/Plug Seal Cap ... Corning™ BioCoat™ Laminin Rectangular Canted Neck Cell Culture Flask w/Plug Seal Cap ...
https://www.fishersci.com/shop/products/corning-biocoat-laminin-rectangular-canted-neck-cell-culture-flask-w-plug-seal-cap-2/p-3016123
Laminin-alpha 2 (LAMA2) / MDC-1A
... laminin-5 (alpha-3 + beta-3 + gamma-2), laminin-6 (alpha-3 + beta-1 + gamma-1), laminin-7 (alpha-3 + beta-2 + gamma-1), laminin ... Laminin-1 (alpha-1 + beta-1 + gamma-1), laminin-2 (merosin, alpha-2 + beta-1 + gamma-1), laminin-3 (alpha-1 + beta-2 + gamma-1 ... In laminins, domains I and II and the C-terminal G domain of the three chains (alpha-2, beta-1 and gamma-1 for laminin-2) ... and laminin-10 (alpha-5 + beta-1 + gamma-1) (Miner et al., 1995). Laminin-2 and -4 are specifically enriched in the basement ...
https://www.dmd.nl/lama2_home.html
SCOP 1.67: Family b.29.1.4: Laminin G-like moduleSCOP 1.67: Family b.29.1.4: Laminin G-like module
More info for Family b.29.1.4: Laminin G-like module. Timeline for Family b.29.1.4: Laminin G-like module: *Family b.29.1.4: ... Family b.29.1.4: Laminin G-like module appears in SCOP 1.65. *Family b.29.1.4: Laminin G-like module appears in SCOP 1.69. * ... Lineage for Family b.29.1.4: Laminin G-like module. *Root: SCOP 1.67 *. Class b: All beta proteins [48724] (141 folds). ... Family b.29.1.4: Laminin G-like module appears in the current release, SCOPe 2.08. ...
http://scop.berkeley.edu/sunid=49944&ver=1.67
Critical Roles for Laminin in Skeletal Muscle Morphogenesis and Myoten . . . by Robert Jones"Critical Roles for Laminin in Skeletal Muscle Morphogenesis and Myoten . . ." by Robert Jones
When laminin mutants are incubated in cyclopamine, to inhibit the hedgehog signaling pathway necessary for slow muscle ... Using genetic mosaic analysis we are able to show that the amount of laminin secreted by transplanted wild-type cells in a ... In both mutants for laminin (31 and yl, and their morpholino oligonucleotide (MO) injected counterparts, we clearly see these ... Embryos deficient for the basement membrane protein laminin exhibit phenotypically shorter, and sometimes round, fast twitch ...
https://digitalcommons.library.umaine.edu/etd/697/
Expression of the 67-kD laminin receptor, galectin-1, and galectin-3 in advanced human uterine adenocarcinoma. | Scholars@DukeExpression of the 67-kD laminin receptor, galectin-1, and galectin-3 in advanced human uterine adenocarcinoma. | [email protected]
Expression of the 67-kD laminin receptor, galectin-1, and galectin-3 in advanced human uterine adenocarcinoma. ... In this study, the authors examined the possibility that expression of three laminin-binding proteins, the 67-kD laminin ... Expression of the 67-kD laminin receptor, galectin-1, and galectin-3 in advanced human uterine adenocarcinoma. Journal Article ... Qualitative and quantitative changes in the expression of cell surface laminin-binding proteins have been correlated with the ...
https://scholars.duke.edu/display/pub679791
Bidirectional signalling between EphA2 and ephrinA1 increases tubular cell attachment, laminin secretion and modulates...Bidirectional signalling between EphA2 and ephrinA1 increases tubular cell attachment, laminin secretion and modulates...
Download PDF Bidirectional signalling between EphA2 and ephrinA1 increases tubular cell attachment, laminin secretion and ... Bidirectional signalling between EphA2 and ephrinA1 increases tubular cell attachment, laminin secretion and modulates ... laminin secretion and modulates erythropoietin expression after renal hypoxic injury. Pflügers Archiv : European Journal of ...
https://www.zora.uzh.ch/id/eprint/124667/
Reactome | Integrins alpha3beta1, alpha6beta4 bind laminin-332, 511, 521, (211, 221)Reactome | Integrins alpha3beta1, alpha6beta4 bind laminin-332, 511, 521, (211, 221)
Laminin interactions (Homo sapiens) * Integrins alpha3beta1, alpha6beta4 bind laminin-332, 511, 521, (211, 221) (Homo sapiens) ... Ligand-binding specificities of laminin-binding integrins: a comprehensive survey of laminin-integrin interactions using ... Regulation of melanoma cell migration and invasion by laminin-5 and alpha3beta1 integrin (VLA-3) Kawada, Y, Irimura, T, Han, SA ... The initial process of laminin (LM) deposition onto the cell surface depends upon interactions with the LG domain located at ...
http://www.reactome.org/content/detail/R-HSA-216048
Anti-Laminin Antibody (M04965) | IHC Validated | BosterbioAnti-Laminin Antibody (M04965) | IHC Validated | Bosterbio
Boster Bio Anti-Laminin Antibody catalog # M04965. Tested in IHC applications. This antibody reacts with Human, Mouse, Rat. ... LAMA; Laminin A chain; laminin subunit alpha-1; laminin, alpha 1; Laminin-1 subunit alpha; Laminin-3 subunit alpha; S-LAM alpha ... S-LAM-alpha laminin subunit alpha 1 laminin subunit alpha-1,S-LAM alpha,S-laminin subunit alpha,laminin A chain,laminin, alpha ... Anti-Laminin Antibody. LAMA1 antibody. Boster Bio Anti-Laminin Antibody catalog # M04965. Tested in IHC applications. This ...
https://www.bosterbio.com/anti-laminin-antibody-m04965-boster.html
High affinity binding between laminin and laminin binding protein of Leishmania is stimulated by zinc and may involve laminin...High affinity binding between laminin and laminin binding protein of Leishmania is stimulated by zinc and may involve laminin...
High affinity binding between laminin and laminin binding protein of Leishmania is stimulated by zinc and may involve laminin ... High affinity binding between laminin and laminin binding protein of Leishmania is stimulated by zinc and may involve laminin ... laminin, a major ECM protein, has been found to bind saturably and with high affinity to a 67-kDa cell surface protein of ... 3-NH2 peptide amide or antibody directed against the 67-kDa laminin-binding protein (LBP) induced tyrosine phosphorylation of ...
http://repository.ias.ac.in/30458/
Reactome | Integrin alpha3beta1, alpha6beta4:Laminins-332, 511, 521, (211, 221) [plasma membrane]Reactome | Integrin alpha3beta1, alpha6beta4:Laminins-332, 511, 521, (211, 221) [plasma membrane]
Laminin interactions (Homo sapiens) * Integrins alpha3beta1, alpha6beta4 bind laminin-332, 511, 521, (211, 221) (Homo sapiens) ... Integrin alpha3beta1, alpha6beta4:Laminins-332, 511, 521, (211, 221) [plasma membrane] Stable Identifier ... Integrin alpha3beta1, alpha6beta4:Laminins-332, 511, 521, (211, 221) [plasma membrane] (Homo sapiens) ... Integrin alpha3beta1, alpha6beta4:Laminins-332, 511, 521, (211, 221) [plasma membrane] (Danio rerio) ...
https://reactome.org/content/detail/R-HSA-3907294
Defining the functional role of laminin isoforms in the regulation of the adult hepatic progenitor cell
HPCs are associated with an increase in laminins in the extracellular matrix. Laminins are heterotrimeric proteins, composed of ... Defining the functional role of laminin isoforms in the regulation of the adult hepatic progenitor cell. View/. Open. ... I examined the laminin alpha chains in two mouse models of HPC activation: a transgenic model using conditional deletion of ... Compared to other laminin chains, Lama5 selectively promotes HPC adhesion and spreading. These effects are partially blocked by ...
https://era.ed.ac.uk/handle/1842/15867
CultreCoat Laminin
... I 96 Well Cell Adhesion Assay. CultreCoat Laminin I Cell Adhesion Assay provides a simple, standardized, ...
https://genoprice.com/cultrecoat_laminin.htm
Earrings - Laminin DesignsEarrings - Laminin Designs
Laminin Designs is a participant in the Amazon Services LLC Associates Program, an affiliate advertising program designed to ...
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laminin - Neuro Probe
Tag: laminin. Posted on October 11, 2012. December 12, 2013. Rapid densitometric determination of cell migration and cell ... AP48, Coomasie, crystal violet, densitometer, fMLP, granulocytes, laminin, melanoma, PBMC A new rapid staining and measuring ...
https://neuroprobe.com/tag/laminin/
Laminin γ2 Mediates Wnt5a-Induced Invasion of Gastric Cancer Cells<...
The invasive activity of gastric cancer cells depended on laminin γ2; Wnt5a expression levels correlated with those of laminin ... The invasive activity of gastric cancer cells depended on laminin γ2; Wnt5a expression levels correlated with those of laminin ... The invasive activity of gastric cancer cells depended on laminin γ2; Wnt5a expression levels correlated with those of laminin ... The invasive activity of gastric cancer cells depended on laminin γ2; Wnt5a expression levels correlated with those of laminin ...
https://okayama.pure.elsevier.com/en/publications/laminin-%CE%B32-mediates-wnt5a-induced-invasion-of-gastric-cancer-cell
laminin Archives - Deana Farrelllaminin Archives - Deana Farrell
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https://deanafarrell.com/tag/laminin/
14mm coverslips laminin coated14mm coverslips laminin coated
60 pieces of CE certified crystal clear German glass coverslips with mouse Laminin coating over PDL layer on both sides of ...
https://www.neuvitro.com/product-page/14mm-coverslips-laminin
Laminin receptors in the retina: Sequence analysis of the chick integrin α6 subunit: Evidence for transcriptional and...
The integrin α6β1 is a prominent laminin receptor used by many cell types. In the present work, we isolate clones and determine ... T1 - Laminin receptors in the retina. T2 - Sequence analysis of the chick integrin α6 subunit: Evidence for transcriptional and ... title = "Laminin receptors in the retina: Sequence analysis of the chick integrin α6 subunit: Evidence for transcriptional and ... Laminin receptors in the retina: Sequence analysis of the chick integrin α6 subunit: Evidence for transcriptional and ...
https://moh-it.pure.elsevier.com/en/publications/laminin-receptors-in-the-retina-sequence-analysis-of-the-chick-in
Human Laminin(LN) ELISA KitHuman Laminin(LN) ELISA Kit
Producing high quality Human Laminin(LN) ELISA Kit products. ... High quality Human Laminin(LN) ELISA Kit from China, Chinas ...
https://www.spanbiotech.com/sale-11685499-human-laminin-ln-elisa-kit.html
Expression of laminin 332 in vessels of oral squamous cell carcinomasExpression of laminin 332 in vessels of oral squamous cell carcinomas
ANGELI, Lorena De et al. Expression of laminin 332 in vessels of oral squamous cell carcinomas. Rev. Bras. Odontol. [online]. ... Palavras-chave : Oral squamous cell carcinomas; Laminin 332; γ2 chain. · resumo em Português · texto em Português · pdf em ... Objective: 1) To analyze the γ2 chain expression of laminin 332 in the basement membrane of vessels of oral squamous cell ... Material and Methods: Immunohistochemistry was performed to detect γ2 chain of laminin 332 in 16 cases of OSCC; of which, eight ...
http://revodonto.bvsalud.org/scielo.php?script=sci_abstract&pid=S0034-72722016000300005&lng=pt&nrm=iso&tlng=en
Changes related to 'Laminin' - Wiki FKKT
Laminin. No changes on linked pages during the given period. Retrieved from "http://wiki.fkkt.uni-lj.si/index.php/Special: ...
http://wiki.fkkt.uni-lj.si/index.php?title=Special:RecentChangesLinked&hideminor=0&days=30&limit=250&hidemyself=1&target=Laminin
Silver Laminin Cross Necklace - Handmado.com
Buy Silver Laminin Cross Necklace from Handmado. Handmade in 925 Sterling Silver with High Quality Craftsmanship. 100% Money ... Silver Laminin Cross Necklace was made for science students, nurses, teachers, doctors and surgeons. Its Unique and Elegance. ...
https://handmado.com/products/silver-laminin-cross-necklace
Laminin - A Message From God? - carolyntorbettjohnsonLaminin - A Message From God? - carolyntorbettjohnson
What is laminin. It is a heterotrimeric (has three units) glycoprotein (proteins that have chains attached to chains). In other ... Laminin. Some people think it shows the way to God, others think it looks like a wilted flower. ... Dysfunctional laminin is linked to congenital muscular dystrophy.. This last picture is of actual laminin molecules. Snopes ... 2 thoughts on "Laminin - A Message From God?" * Raphael Jordan December 26, 20174:44 am Reply ...
https://carolyntorbettjohnson.com/2015/08/01/luminin-a-message-from-god-2/?replytocom=404
rHu Laminin-332 | Technique alternative | 01010665761 - Gentaur shop
Order rHu Laminin-332 01010665761 at Gentaur rHu Laminin-332 ... rHu Laminin-332. Basic and detailed information about product: ...
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Laminin-Mediated interactions in Thymocyte Migration and DevelopmentLaminin-Mediated interactions in Thymocyte Migration and Development
... Savino, Wilson et al. , Date Issued: 2015 ... Laminin-Mediated Interactions in Thymocyte Migration and Development. Frontiers in Immunology, v.6, Article 579, 13p, Nov. 2015 ... One of the individual vectors comprises interactions mediated by laminins (LMs), a heterotrimeric protein family of the ...
https://www.arca.fiocruz.br/handle/icict/13776
DSC 0156 - LaNts and LamininsDSC 0156 - LaNts and Laminins
Follow LaNts and Laminins on WordPress.com Recent Posts. * New paper published - LaNts and laminin polymerisation review ...
https://lantsandlaminins.com/2017/05/16/sunscreen-challenge-1-meet-the-scientists-may-2017/dsc_0156/
Mark 10:48 (Bound with Laminin) - LIFE Runners Team
27 May Mark 10:48 (Bound with Laminin). Posted on Thursday 27 May, 2010 Share. ... Thank you Lord for holding us all together, our individual bodies (Laminin) and the Body of Christ (Church)…Amen. ... Deanna Peters shared this inspiring video based on Laminin, which is a cross-shaped molecule that holds the body together. ...
https://www.liferunners.org/mark-1048-bound-with-laminin/
Nacalai USA, Inc. | Product | iMatrix-511(recombinant laminin-511)Nacalai USA, Inc. | Product | iMatrix-511(recombinant laminin-511)
Laminin E8 fragments support efficient adhesion and expansion of dissociated human pluripotent stem cells. Nat. Commun. 3: 1236 ... iMatrix-511(recombinant laminin-511). Cell culture substrate / Recombinant human Laminin511-E8 fragment ... Home , Products , Life Sciences , Cell Biology , Extracellular Matrix (ECM) , iMatrix-511(recombinant laminin-511) ... Efficient Adhesion Culture of Human Pluripotent Stem Cells Using Laminin Fragments in an Uncoated Manner. Sci. Rep. 7: 41165, ...
https://www.nacalaiusa.com/products/view/354/imatrix-511
ExtracellularRecombinantProteinsHeterotrimericProteinReceptorIsoformsAnti-lamininAntibodyMerosinReceptorsSubstratesLAMC1DifferentiationAntibodiesInteractionsGeneBindMuscular DystrophyChainAlpha6beta4TumorCollagenAdhesionCellsActinDeficiencyBasement membranesLiverExpressionChainsInhibitComponentVivoElectronSignificantlyMoleculeAlpha
Extracellular12
  • Laminins, a family of extracellular matrix glycoproteins, are the major noncollagenous constituent of basement membranes. (nih.gov)
  • In the course of trying to understand the pathogenesis of leishmaniasis in relation to extracellular matrix (ECM) elements, laminin, a major ECM protein, has been found to bind saturably and with high affinity to a 67-kDa cell surface protein of Leishmania donovani. (ias.ac.in)
  • HPCs are associated with an increase in laminins in the extracellular matrix. (ed.ac.uk)
  • Laminin binds to other cell membrane and extracellular matrix molecules. (carolyntorbettjohnson.com)
  • One of the individual vectors comprises interactions mediated by laminins (LMs), a heterotrimeric protein family of the extracellular matrix. (fiocruz.br)
  • Members of both integrin and laminin families are known to be involved in cell-extracellular matrix adhesion and have been implicated in tumor metastatic processes in several cancers. (who.int)
  • The extracellular-matrix protein laminin forms polymers both in vivo and in vitro . (bvsalud.org)
  • Follicle cells in the thyroid are in close contact with laminin , but their response to this important extracellular signal is still poorly understood. (bvsalud.org)
  • We have previously shown that lupus autoantibodies bind to the laminin component of the extracellular matrix. (uthscsa.edu)
  • We have synthesized a panel of peptides that cross-react with the anti-laminin Abs and have found that the binding of lupus autoantibodies to the extracellular matrix could be inhibited in vitro by some of these competitive peptides. (uthscsa.edu)
  • Cisplatin, mefloquine, and cadmium significantly reduced the expression of actin and laminin, in the extracellular matrix, leading to significant disarray of the sensory epithelium. (cdc.gov)
  • Laminins are extracellular glycoproteins that bind with other extracellular and transmembrane proteins to form the frame of the basal lamina that surrounds individual myofibers. (medscape.com)
Recombinant3
  • Recombinant integrins vary in their laminin specificities: integrins alpha3beta1 and alpha6beta4 have a clear specificity for LM-332 and -511/512, integrin alpha6beta1 has a broad specificity, binding all LM isoforms with a preference for LM-111, -332 and -511/521. (reactome.org)
  • I then studied the functional effects of matrix on cell behaviour in vitro using recombinant laminins and a line of spontaneously immortalised mouse HPCs. (ed.ac.uk)
  • With our human recombinant laminin substrates, Biolaminin®, pluripotent stem cells, adult stem cells, and tissue-specific cells can be cultured in a physiologically relevant environment, making cell culture more reliable and robust. (biolamina.com)
Proteins8
  • Laminins are a family of large trimeric basement membrane glyco-proteins composed of 3 non-identical chains, the alpha-, beta- and gamma-chains. (dmd.nl)
  • Qualitative and quantitative changes in the expression of cell surface laminin-binding proteins have been correlated with the ability of cancer cells to cross basement membranes during the metastatic cascade. (duke.edu)
  • In this study, the authors examined the possibility that expression of three laminin-binding proteins, the 67-kD laminin receptor (67LR), galectin-1, and galectin-3, is altered in human endometrial cancer in a fashion similar to that reported in other carcinomas, such as breast, colon, and ovarian cancer. (duke.edu)
  • Further studies on larger populations should determine the prognostic value of the detection of these laminin-binding proteins in endometrial carcinoma. (duke.edu)
  • The strong band above the 280kDa mark corresponds to full length laminin proteins. (bosterbio.com)
  • Furthermore, incubation of L. donovani promastigotes with C(YIGSR) 3 -NH 2 peptide amide or antibody directed against the 67-kDa laminin-binding protein (LBP) induced tyrosine phosphorylation of proteins with a molecular mass ranging from 115 to 130 kDa. (ias.ac.in)
  • Laminins are heterotrimeric proteins, composed of an alpha, beta and gamma chain. (ed.ac.uk)
  • Laminins are a family of proteins that are an integral part of the structural basis of membranes in almost every animal tissue. (thenatureinus.com)
Heterotrimeric1
  • Different alpha, beta and gamma chain isomers combine to give rise to different heterotrimeric laminin isoforms which are designated by Arabic numerals in the order of their discovery, i.e. alpha1beta1gamma1 heterotrimer is laminin 1. (nih.gov)
Protein14
  • Each laminin chain is a multidomain protein encoded by a distinct gene. (nih.gov)
  • Each laminin chain is a multi-domain protein encoded by a distinct gene. (dmd.nl)
  • Embryos deficient for the basement membrane protein laminin exhibit phenotypically shorter, and sometimes round, fast twitch muscle cells, disrupted slow fiber migration and they often lack the ability to maintain myotome boundary (tendon) integrity. (umaine.edu)
  • This study shows an alteration of nonintegrin laminin-binding protein expression in advanced human endometrial cancer. (duke.edu)
  • Immunohistological analysis of brain stem section stained with rabbit pAb to laminin, M04965, dilution 1:1,000 in red, and costained with chicken pAb to Myelin Basic Protein (MBP), dilution 1:5,000 in green. (bosterbio.com)
  • Microarray analyses indicated that Wnt5a activity induced expression of the gene encoding laminin γ2, a subunit of the epithelial basement membrane protein laminin-5. (elsevier.com)
  • Wnt5a induced the expression of laminin γ2 through the activation of protein kinase C and c-Jun-N-terminal kinase. (elsevier.com)
  • In retinal ganglion cells, we show that this is correlated with a dramatic decrease in α 6 mRNA and protein, suggesting that changes in gene expression account for the developmental regulation of the interactions of these neurons with laminin. (elsevier.com)
  • Solid phase assays demonstrate that B-CAM/LU binds laminin on intact sickle red cells and that red cell B-CAM/LU binds immobilized laminin, whereas another putative laminin binding protein, CD44, does not. (duke.edu)
  • Ligand blots also identify B-CAM/LU as the only erythrocyte membrane protein(s) that binds laminin. (duke.edu)
  • Thus, B-CAM/LU appears to be the major laminin-binding protein of sickle red cells. (duke.edu)
  • Previously reported overexpression of B-CAM/LU by epithelial cancer cells suggests that this protein may also serve as a laminin receptor in malignant tumors. (duke.edu)
  • The laminin γ1 chain (LAMC1) is a core structural protein present in the basement membrane of several organs, including the kidneys. (nordicbioscience.com)
  • Cumulatively, these data provide a reference method to control and screen for i-Hep differentiation, identify Laminin 411 as a key niche protein, and underscore the importance of combining substrates, soluble factors, and HCA when developing iPSC applications. (ox.ac.uk)
Receptor4
  • Expression of the 67-kD laminin receptor, galectin-1, and galectin-3 in advanced human uterine adenocarcinoma. (duke.edu)
  • The integrin α 6 β 1 is a prominent laminin receptor used by many cell types. (elsevier.com)
  • abstract = "The integrin α6β1 is a prominent laminin receptor used by many cell types. (elsevier.com)
  • The receptor critical for sickle cell adhesion to laminin. (duke.edu)
Isoforms1
  • The different laminin isoforms consist of different combinations of the alpha-, beta- and gamma-chains which are designated by Arabic numerals in the order of their discovery. (dmd.nl)
Anti-laminin1
  • Boster Bio Anti-Laminin Antibody catalog # M04965. (bosterbio.com)
Antibody5
  • Smaller proteolytic fragments of laminin are also detected with this antibody. (bosterbio.com)
  • The laminin antibody is an excellent marker of basement membranes surrounding blood vessels, while the MBP antibody stains the myelin sheathes around axons. (bosterbio.com)
  • In cell adhesion assays, monoclonal antibody AL-2 inhibited the binding of the highly metastatic melanoma cell line, K-1735-M4, to both intact laminin and the 440-kDa fragment of laminin. (umn.edu)
  • Electron microscopic examination of laminin-monoclonal antibody interactions showed that monoclonal antibody AL-2 reacted with the long arm of laminin directly below the cross-region. (umn.edu)
  • Using antibody immunohistochemistry, cross-sections (thickness 8 um) of distal parts of gastrocnemius medialis muscle were analyzed for desmin, albumin, laminin and dystrophin. (cdc.gov)
Merosin1
  • The major laminin of adult skeletal muscle is laminin-2 (also known as merosin), and only mutations of LAMA2 gene encoding laminin α2 cause muscular dystrophy. (medscape.com)
Receptors1
  • rLN-alpha2G mediated M. leprae binding to cell lines and to sciatic nerves of dystrophic dy/dy mice lacking LN-alpha2, but expressing laminin receptors. (ed.ac.uk)
Substrates1
  • Iodide uptake decreased similarly in response to both laminin substrates , in comparison to glass . (bvsalud.org)
LAMC11
  • Laminin-111 expression (composed of LAMA1, LAMB1, and LAMC1) was found to correlate with cell differentiation in oral cancer, showing a gradual decrease from well differentiated to poorly differentiated cases. (who.int)
Differentiation2
  • Kulkarni S, Abdulla R, Jose M, Adyanthaya S, B-Rex DA, Patil AH, Pinto SM, Subbannayya Y. Omics data-driven analysis identifies laminin-integrin-mediated signaling pathway as a determinant for cell differentiation in oral squamous cell carcinoma. (who.int)
  • Although Lindahl didn't record on differentiation among different severities Laminin (925-933) of burn off wounds their data recommend the power of LSI to monitor the curing of burn off wounds inside a medical placing. (careersfromscience.org)
Antibodies3
  • Monoclonal antibodies were prepared to localize the domain(s) of laminin to which tumor cells adhere. (umn.edu)
  • Three monoclonal antibodies (AL-1 to AL-3) that bound to intact laminin in a solid-phase radioimmunoassay were chosen for further analysis. (umn.edu)
  • Two monoclonal antibodies that failed to inhibit tumor cell adhesion to laminin reacted with epitopes on the lateral short arms or cross-region of laminin as seen by electron microscopy. (umn.edu)
Interactions3
  • Alterations of tumor cell interactions with laminin, a basement membrane glycoprotein, are consistent features of the invasive and metastatic phenotype. (duke.edu)
  • The initial process of laminin (LM) deposition onto the cell surface depends upon interactions with the LG domain located at the alpha chain C-terminus. (reactome.org)
  • CultreCoat Laminin I Cell Adhesion Assay provides a simple, standardized, high throughput format for assessing factors that influence cell-matrix interactions. (genoprice.com)
Gene5
  • This gene encodes the gamma chain isoform laminin, gamma 3. (nih.gov)
  • Mutations in the gamma 2 chain gene (LAMC2) of kalinin/laminin 5 in the junctional forms of epidermolysis bullosa. (medscape.com)
  • MDC1A is caused by mutations in the laminin α2 gene ( LAMA2 ) linked to chromosome 6q22-q23 and inherited as autosomal recessive. (medscape.com)
  • Caused by mutations in the LAMA2 gene coding for the alpha-2 laminin chain. (cdc.gov)
  • Is laminin gamma-1 a candidate gene for advanced pelvic organ prolapse? (cdc.gov)
Bind3
  • LAMA2 has been shown to bind to dystroglycan , which interacts with dystrophin and extra-cellular laminin. (dmd.nl)
  • The three short arms bind to other laminin molecules to form sheets. (carolyntorbettjohnson.com)
  • Sickle red cells bind significant amounts of soluble laminin, whereas normal red cells do not. (duke.edu)
Muscular Dystrophy1
Chain7
  • Laminins are composed of 3 non identical chains: laminin alpha, beta and gamma (formerly A, B1, and B2, respectively) and they form a cruciform structure consisting of 3 short arms, each formed by a different chain, and a long arm composed of all 3 chains. (nih.gov)
  • The laminin alpha 5 (Lama5) chain is significantly upregulated in both models and forms a basement membrane which surrounds the progenitor cells. (ed.ac.uk)
  • Objective: 1) To analyze the γ2 chain expression of laminin 332 in the basement membrane of vessels of oral squamous cell carcinoma (OSCC), and the appearance of continuity, to identify possible vascular invasive procedures which are important events in the disease progression. (bvsalud.org)
  • We have found that pathogenic murine lupus autoantibodies react with a 21-mer peptide located in the globular part of the α-chain of laminin. (uthscsa.edu)
  • Neural targeting of Mycobacterium leprae mediated by the G domain of the laminin-alpha2 chain. (ed.ac.uk)
  • We report that the molecular basis of the neural tropism of Mycobacterium leprae is attributable to the specific binding of M. leprae to the laminin-alpha2 (LN-alpha2) chain on Schwann cell-axon units. (ed.ac.uk)
  • Each laminin is a heterodimer composed of a heavy chain (α) and two light chains (β and γ). (medscape.com)
Alpha6beta41
  • 2003. Autocrine laminin-5 ligates alpha6beta4 integrin and activates RAC and NFkappaB to mediate anchorage-independent survival of mammary tumors. . (ucsf.edu)
Tumor2
  • Wnt5a expression levels correlated with those of laminin γ2 in diffuse-scattered type gastric tumor samples from patients. (elsevier.com)
  • These results suggest that a new tumor cell binding domain of laminin may be located close to the cross-region on the long arm of laminin. (umn.edu)
Collagen1
  • The hydrogels come with several types of functional ligand modifications such as RGD (VitroGel RGD), Collagen (VitroGel COL), Laminin (VitroGel IKVAV & VitroGel YIGSR) and MMP (VitroGel MMP). (lab-a-porter.com)
Adhesion2
  • In both mutants for laminin (31 and yl, and their morpholino oligonucleotide (MO) injected counterparts, we clearly see these shorter muscle cells which demonstrates the importance of laminin's role in cell-matrix adhesion during myotome morphogenesis. (umaine.edu)
  • Compared to other laminin chains, Lama5 selectively promotes HPC adhesion and spreading. (ed.ac.uk)
Cells6
  • The distribution of gamma 3-containing laminins along ciliated epithelial surfaces suggests that the apical laminins are important in the morphogenesis and structural stability of the ciliated processes of these cells. (nih.gov)
  • Using genetic mosaic analysis we are able to show that the amount of laminin secreted by transplanted wild-type cells in a mutant embryo is insufficient to rescue fast fiber elongation, though the transplanted cells themselves are capable of elongating. (umaine.edu)
  • PCCL3 thyroid follicular cells cultured on glass , on regular laminin (LM) or on laminin previously polymerized in acidic pH (polyLM) showed different cell morphologies and propensities to proliferate, as well as differences in the organization of their actin cytoskeleton . (bvsalud.org)
  • Rat Y3-Ag 1.2.3 myeloma cells were fused with spleen cells from a rat immunized with a purified 440-kDa fragment of chymotrypsin-digested laminin. (umn.edu)
  • Finally, transfection of murine erythroleukemia cells with human B-CAM cDNA induces binding of both soluble and immobilized laminin. (duke.edu)
  • Almost all cells in the human body grow on specific laminins. (biolamina.com)
Actin1
  • The loss of laminin 332 from the skin basement membrane disrupts the actin cytoskeleton which is vital for the movement of cholesterol within the skin. (eb-researchnetwork.org)
Deficiency3
  • Laminin 411, the top hit, was validated in two additional induced pluripotent stem cell (iPSC) lines, primary tissue, and an in vitro model of α1-antitrypsin deficiency. (ox.ac.uk)
  • Clinical variants of MDC1A occur with some mutations when only partial laminin-α2 deficiency is present. (medscape.com)
  • Clues to the presence of laminin-α2 deficiency include MRI abnormalities, seizures, and demyelinating neuropathy. (medscape.com)
Basement membranes1
  • Laminin-2 and -4 are specifically enriched in the basement membranes surrounding skeletal muscle fibers and peripheral nerves. (dmd.nl)
Liver1
  • Laminin alpha 5-containing matrix is deposited around HPCs during liver regeneration and supports progenitor cell attachment, migration and maintenance of an undifferentiated phenotype. (ed.ac.uk)
Expression1
  • There was also a significant increase in hepatocellular injury and fibrosis in response to the DDC diet seen with loss of laminin alpha 5 expression. (ed.ac.uk)
Chains2
  • My aims were to describe the laminin alpha chains associated with the HPC response and to define the functional effects of specific laminin chains on HPCs. (ed.ac.uk)
  • I examined the laminin alpha chains in two mouse models of HPC activation: a transgenic model using conditional deletion of Mdm2 in hepatocytes, and a dietary model using 3,5-diethoxycarbonyl-1,4-dihydrocollidine (DDC). (ed.ac.uk)
Inhibit1
  • When laminin mutants are incubated in cyclopamine, to inhibit the hedgehog signaling pathway necessary for slow muscle specification, fast fibers fail to recover at all and the myotome boundaries are intact. (umaine.edu)
Component2
  • It is a component of laminin 12. (nih.gov)
  • The most severe form, JEB generalised severe, is caused by complete loss of one of the parts of laminin 332 a key component of the basement membrane. (eb-researchnetwork.org)
Vivo1
  • I then studied the effects in vivo using transgenic Cre-lox mouse strains that allow conditional knock-out of either laminin alpha 5 or beta-1 integrin in HPCs. (ed.ac.uk)
Electron1
Significantly2
  • Binding activity was significantly enhanced by Zn 2+ , an effect possibly mediated through Cys-rich zinc finger-like sequences on laminin. (ias.ac.in)
  • Additionally, through association evidence and network analysis, we also identified members of the laminin family to be significantly altered in head and neck cancers. (who.int)
Molecule2
  • Deanna Peters shared this inspiring video based on Laminin , which is a cross-shaped molecule that holds the body together. (liferunners.org)
  • In the present work, we have analyzed the fine specificity of the interaction of pathogenic murine lupus autoantibodies with this molecule and the effect of inhibiting their binding to laminin during the course of the disease. (uthscsa.edu)
Alpha2
  • Laminin-alpha 2 is widely expressed in the basement membrane of skeletal muscle, at neuromuscular synpases, extra-synaptically and in the myotendinous junctions. (dmd.nl)
  • Although these experiments were limited by a low number of experimental animals and low recombination rates, there was a suggestion of impaired HPC expansion associated with loss of laminin alpha 5. (ed.ac.uk)
RPSA gene: MedlinePlus Genetics
RPSA gene: MedlinePlus Genetics (medlineplus.gov)
Regulation of cellular senescence by extracellular matrix during chronic fibrotic diseases | Clinical Science | Portland Press
Regulation of cellular senescence by extracellular matrix during chronic fibrotic diseases | Clinical Science | Portland Press (portlandpress.com)
Bullous Pemphigoid: Practice Essentials, Background, Pathophysiology
Bullous Pemphigoid: Practice Essentials, Background, Pathophysiology (medscape.com)
Pediatric Epidermolysis Bullosa Workup: Laboratory Studies, Imaging Studies, Histologic Findings
Pediatric Epidermolysis Bullosa Workup: Laboratory Studies, Imaging Studies, Histologic Findings (emedicine.medscape.com)
Ciglitazone - Ontology Report - Rat Genome Database
Ciglitazone - Ontology Report - Rat Genome Database (rgd.mcw.edu)
Limb-Girdle Muscular Dystrophy Clinical Presentation: History, Causes
Limb-Girdle Muscular Dystrophy Clinical Presentation: History, Causes (medscape.com)
MedlinePlus: Genetic Conditions: L
MedlinePlus: Genetic Conditions: L (medlineplus.gov)
RhoA Antibody (Mouse Monoclonal) - Cytoskeleton, Inc.
RhoA Antibody (Mouse Monoclonal) - Cytoskeleton, Inc. (cytoskeleton.com)
Live Cell Reagents Probes Fluorescent
Live Cell Reagents Probes Fluorescent (cytoskeleton.com)
Proteomes | Free Full-Text | Differential Proteome Analysis of Extracellular Vesicles from Breast Cancer Cell Lines by...
Proteomes | Free Full-Text | Differential Proteome Analysis of Extracellular Vesicles from Breast Cancer Cell Lines by... (mdpi.com)
Normal huntingtin function: an alternative approach to Huntington's disease | Nature Reviews Neuroscience
Normal huntingtin function: an alternative approach to Huntington's disease | Nature Reviews Neuroscience (nature.com)
Cells | Free Full-Text | Nerve, Muscle, and Synaptogenesis
Cells | Free Full-Text | Nerve, Muscle, and Synaptogenesis (mdpi.com)
Recombinant Macaca mulatta Interleukin-1 alpha (IL1A) | CSB-YP011613MOW | Cusabio
Recombinant Macaca mulatta Interleukin-1 alpha (IL1A) | CSB-YP011613MOW | Cusabio (joplink.net)
A narrative overview of utilizing biomaterials to recapitulate the salient regenerative features of dental-derived mesenchymal...
A narrative overview of utilizing biomaterials to recapitulate the salient regenerative features of dental-derived mesenchymal... (nature.com)
YSP Research Projects - UC Davis School of Education
YSP Research Projects - UC Davis School of Education (education.ucdavis.edu)
A basement membrane discovery pipeline uncovers network complexity, regulators, and human disease associations | Science...
A basement membrane discovery pipeline uncovers network complexity, regulators, and human disease associations | Science... (science.org)
MBL Scientist | Marine Biological Laboratory
MBL Scientist | Marine Biological Laboratory (mbl.edu)
Parenchymal border macrophages regulate the flow dynamics of the cerebrospinal fluid | Nature
Parenchymal border macrophages regulate the flow dynamics of the cerebrospinal fluid | Nature (nature.com)
Congenital Myopathies Clinical Presentation: History, Nemaline Rod Myopathy, Core Myopathy
Congenital Myopathies Clinical Presentation: History, Nemaline Rod Myopathy, Core Myopathy (medscape.com)