Large, noncollagenous glycoprotein with antigenic properties. It is localized in the basement membrane lamina lucida and functions to bind epithelial cells to the basement membrane. Evidence suggests that the protein plays a role in tumor invasion.
Glycoprotein molecules on the surface of cells that react with or bind to laminin whose function allows the binding of epithelial cells to the basement membrane. The molecular weight of this high-affinity receptor is 67 kD.
A darkly stained mat-like EXTRACELLULAR MATRIX (ECM) that separates cell layers, such as EPITHELIUM from ENDOTHELIUM or a layer of CONNECTIVE TISSUE. The ECM layer that supports an overlying EPITHELIUM or ENDOTHELIUM is called basal lamina. Basement membrane (BM) can be formed by the fusion of either two adjacent basal laminae or a basal lamina with an adjacent reticular lamina of connective tissue. BM, composed mainly of TYPE IV COLLAGEN; glycoprotein LAMININ; and PROTEOGLYCAN, provides barriers as well as channels between interacting cell layers.
Glycoproteins found on the surfaces of cells, particularly in fibrillar structures. The proteins are lost or reduced when these cells undergo viral or chemical transformation. They are highly susceptible to proteolysis and are substrates for activated blood coagulation factor VIII. The forms present in plasma are called cold-insoluble globulins.
Adherence of cells to surfaces or to other cells.
A cell surface receptor mediating cell adhesion to the EXTRACELLULAR MATRIX and to other cells via binding to LAMININ. It is involved in cell migration, embryonic development, leukocyte activation and tumor cell invasiveness. Integrin alpha6beta1 is the major laminin receptor on PLATELETS; LEUKOCYTES; and many EPITHELIAL CELLS, and ligand binding may activate a number of signal transduction pathways. Alternative splicing of the cytoplasmic domain of the alpha6 subunit (INTEGRIN ALPHA6) results in the formation of A and B isoforms of the heterodimer, which are expressed in a tissue-specific manner.
A family of transmembrane glycoproteins (MEMBRANE GLYCOPROTEINS) consisting of noncovalent heterodimers. They interact with a wide variety of ligands including EXTRACELLULAR MATRIX PROTEINS; COMPLEMENT, and other cells, while their intracellular domains interact with the CYTOSKELETON. The integrins consist of at least three identified families: the cytoadhesin receptors(RECEPTORS, CYTOADHESIN), the leukocyte adhesion receptors (RECEPTORS, LEUKOCYTE ADHESION), and the VERY LATE ANTIGEN RECEPTORS. Each family contains a common beta-subunit (INTEGRIN BETA CHAINS) combined with one or more distinct alpha-subunits (INTEGRIN ALPHA CHAINS). These receptors participate in cell-matrix and cell-cell adhesion in many physiologically important processes, including embryological development; HEMOSTASIS; THROMBOSIS; WOUND HEALING; immune and nonimmune defense mechanisms; and oncogenic transformation.
A meshwork-like substance found within the extracellular space and in association with the basement membrane of the cell surface. It promotes cellular proliferation and provides a supporting structure to which cells or cell lysates in culture dishes adhere.
Dystrophin-associated proteins that play role in the formation of a transmembrane link between laminin-2 and DYSTROPHIN. Both the alpha and the beta subtypes of dystroglycan originate via POST-TRANSLATIONAL PROTEIN PROCESSING of a single precursor protein.
A polypeptide substance comprising about one third of the total protein in mammalian organisms. It is the main constituent of SKIN; CONNECTIVE TISSUE; and the organic substance of bones (BONE AND BONES) and teeth (TOOTH).
An integrin alpha subunit that primarily associates with INTEGRIN BETA1 or INTEGRIN BETA4 to form laminin-binding heterodimers. Integrin alpha6 has two alternatively spliced isoforms: integrin alpha6A and integrin alpha6B, which differ in their cytoplasmic domains and are regulated in a tissue-specific and developmental stage-specific manner.
Cell surface receptor for LAMININ, epiligrin, FIBRONECTINS, entactin, and COLLAGEN. Integrin alpha3beta1 is the major integrin present in EPITHELIAL CELLS, where it plays a role in the assembly of BASEMENT MEMBRANE as well as in cell migration, and may regulate the functions of other integrins. Two alternatively spliced isoforms of the alpha subunit (INTEGRIN ALPHA3), are differentially expressed in different cell types.
A complex blood group system having pairs of alternate antigens and amorphic genes, but also subject to a dominant independently segregating repressor.
Macromolecular organic compounds that contain carbon, hydrogen, oxygen, nitrogen, and usually, sulfur. These macromolecules (proteins) form an intricate meshwork in which cells are embedded to construct tissues. Variations in the relative types of macromolecules and their organization determine the type of extracellular matrix, each adapted to the functional requirements of the tissue. The two main classes of macromolecules that form the extracellular matrix are: glycosaminoglycans, usually linked to proteins (proteoglycans), and fibrous proteins (e.g., COLLAGEN; ELASTIN; FIBRONECTINS; and LAMININ).
Integrin beta-1 chains which are expressed as heterodimers that are noncovalently associated with specific alpha-chains of the CD49 family (CD49a-f). CD29 is expressed on resting and activated leukocytes and is a marker for all of the very late activation antigens on cells. (from: Barclay et al., The Leukocyte Antigen FactsBook, 1993, p164)
A non-fibrillar collagen found in the structure of BASEMENT MEMBRANE. Collagen type IV molecules assemble to form a sheet-like network which is involved in maintaining the structural integrity of basement membranes. The predominant form of the protein is comprised of two alpha1(IV) subunits and one alpha2(IV) subunit, however, at least six different alpha subunits can be incorporated into the heterotrimer.
This intrgrin is a key component of HEMIDESMOSOMES and is required for their formation and maintenance in epithelial cells. Integrin alpha6beta4 is also found on thymocytes, fibroblasts, and Schwann cells, where it functions as a laminin receptor (RECEPTORS, LAMININ) and is involved in wound healing, cell migration, and tumor invasiveness.
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others.
Surface ligands, usually glycoproteins, that mediate cell-to-cell adhesion. Their functions include the assembly and interconnection of various vertebrate systems, as well as maintenance of tissue integration, wound healing, morphogenic movements, cellular migrations, and metastasis.
The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.
Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
The movement of cells from one location to another. Distinguish from CYTOKINESIS which is the process of dividing the CYTOPLASM of a cell.
Histochemical localization of immunoreactive substances using labeled antibodies as reagents.
An integrin alpha subunit that occurs as alternatively spliced isoforms. The isoforms are differentially expressed in specific cell types and at specific developmental stages. Integrin alpha3 combines with INTEGRIN BETA1 to form INTEGRIN ALPHA3BETA1 which is a heterodimer found primarily in epithelial cells.
Cell surface molecules on cells of the immune system that specifically bind surface molecules or messenger molecules and trigger changes in the behavior of cells. Although these receptors were first identified in the immune system, many have important functions elsewhere.
The process in which substances, either endogenous or exogenous, bind to proteins, peptides, enzymes, protein precursors, or allied compounds. Specific protein-binding measures are often used as assays in diagnostic assessments.
Partial proteins formed by partial hydrolysis of complete proteins or generated through PROTEIN ENGINEERING techniques.
Established cell cultures that have the potential to propagate indefinitely.
Microscopy using an electron beam, instead of light, to visualize the sample, thereby allowing much greater magnification. The interactions of ELECTRONS with specimens are used to provide information about the fine structure of that specimen. In TRANSMISSION ELECTRON MICROSCOPY the reactions of the electrons that are transmitted through the specimen are imaged. In SCANNING ELECTRON MICROSCOPY an electron beam falls at a non-normal angle on the specimen and the image is derived from the reactions occurring above the plane of the specimen.
In tissue culture, hairlike projections of neurons stimulated by growth factors and other molecules. These projections may go on to form a branched tree of dendrites or a single axon or they may be reabsorbed at a later stage of development. "Neurite" may refer to any filamentous or pointed outgrowth of an embryonal or tissue-culture neural cell.
Ubiquitous macromolecules associated with the cell surface and extracellular matrix of a wide range of cells of vertebrate and invertebrate tissues. They are essential cofactors in cell-matrix adhesion processes, in cell-cell recognition systems, and in receptor-growth factor interactions. (From Cancer Metastasis Rev 1996; 15(2): 177-86; Hepatology 1996; 24(3): 524-32)
Cells grown in vitro from neoplastic tissue. If they can be established as a TUMOR CELL LINE, they can be propagated in cell culture indefinitely.
Collagen receptors are cell surface receptors that modulate signal transduction between cells and the EXTRACELLULAR MATRIX. They are found in many cell types and are involved in the maintenance and regulation of cell shape and behavior, including PLATELET ACTIVATION and aggregation, through many different signaling pathways and differences in their affinities for collagen isoforms. Collagen receptors include discoidin domain receptors, INTEGRINS, and glycoprotein VI.
Glycoproteins found on the membrane or surface of cells.
Conjugated protein-carbohydrate compounds including mucins, mucoid, and amyloid glycoproteins.
A highly acidic mucopolysaccharide formed of equal parts of sulfated D-glucosamine and D-glucuronic acid with sulfaminic bridges. The molecular weight ranges from six to twenty thousand. Heparin occurs in and is obtained from liver, lung, mast cells, etc., of vertebrates. Its function is unknown, but it is used to prevent blood clotting in vivo and vitro, in the form of many different salts.
One or more layers of EPITHELIAL CELLS, supported by the basal lamina, which covers the inner or outer surfaces of the body.
Works containing information articles on subjects in every field of knowledge, usually arranged in alphabetical order, or a similar work limited to a special field or subject. (From The ALA Glossary of Library and Information Science, 1983)
Glycoproteins which contain sialic acid as one of their carbohydrates. They are often found on or in the cell or tissue membranes and participate in a variety of biological activities.
Progressive restriction of the developmental potential and increasing specialization of function that leads to the formation of specialized cells, tissues, and organs.
Methods for maintaining or growing CELLS in vitro.
A subcomponent of complement C1, composed of six copies of three polypeptide chains (A, B, and C), each encoded by a separate gene (C1QA; C1QB; C1QC). This complex is arranged in nine subunits (six disulfide-linked dimers of A and B, and three disulfide-linked homodimers of C). C1q has binding sites for antibodies (the heavy chain of IMMUNOGLOBULIN G or IMMUNOGLOBULIN M). The interaction of C1q and immunoglobulin activates the two proenzymes COMPLEMENT C1R and COMPLEMENT C1S, thus initiating the cascade of COMPLEMENT ACTIVATION via the CLASSICAL COMPLEMENT PATHWAY.
A rare myeloproliferative disorder that is characterized by a sustained, mature neutrophilic leukocytosis. No monocytosis, EOSINOPHILIA, or basophilia is present, nor is there a PHILADELPHIA CHROMOSOME or bcr-abl fusion gene (GENES, ABL).
Members of the class of compounds composed of AMINO ACIDS joined together by peptide bonds between adjacent amino acids into linear, branched or cyclical structures. OLIGOPEPTIDES are composed of approximately 2-12 amino acids. Polypeptides are composed of approximately 13 or more amino acids. PROTEINS are linear polypeptides that are normally synthesized on RIBOSOMES.
Single or multi-sheet notices made to attract attention to events, activities, causes, goods, or services. They are for display, usually in a public place and are chiefly pictorial.
A republic in central Africa south of CHAD and SUDAN, north of DEMOCRATIC REPUBLIC OF THE CONGO, and east of CAMEROON. The capital is Bangui.
Time period from 1901 through 2000 of the common era.
A monocyclic beta-lactam antibiotic originally isolated from Chromobacterium violaceum. It is resistant to beta-lactamases and is used in gram-negative infections, especially of the meninges, bladder, and kidneys. It may cause a superinfection with gram-positive organisms.
Cytoplasmic proteins that bind estrogens and migrate to the nucleus where they regulate DNA transcription. Evaluation of the state of estrogen receptors in breast cancer patients has become clinically important.
Tumors or cancer of the human BREAST.
An invasive (infiltrating) CARCINOMA of the mammary ductal system (MAMMARY GLANDS) in the human BREAST.
Breast neoplasms that do not express ESTROGEN RECEPTORS; PROGESTERONE RECEPTORS; and do not overexpress the NEU RECEPTOR/HER-2 PROTO-ONCOGENE PROTEIN.
A malignant neoplasm made up of epithelial cells tending to infiltrate the surrounding tissues and give rise to metastases. It is a histological type of neoplasm but is often wrongly used as a synonym for "cancer." (From Dorland, 27th ed)
In humans, one of the paired regions in the anterior portion of the THORAX. The breasts consist of the MAMMARY GLANDS, the SKIN, the MUSCLES, the ADIPOSE TISSUE, and the CONNECTIVE TISSUES.

Inhibition of in vitro enteric neuronal development by endothelin-3: mediation by endothelin B receptors. (1/4299)

The terminal colon is aganglionic in mice lacking endothelin-3 or its receptor, endothelin B. To analyze the effects of endothelin-3/endothelin B on the differentiation of enteric neurons, E11-13 mouse gut was dissociated, and positive and negative immunoselection with antibodies to p75(NTR )were used to isolate neural crest- and non-crest-derived cells. mRNA encoding endothelin B was present in both the crest-and non-crest-derived cells, but that encoding preproendothelin-3 was detected only in the non-crest-derived population. The crest- and non-crest-derived cells were exposed in vitro to endothelin-3, IRL 1620 (an endothelin B agonist), and/or BQ 788 (an endothelin B antagonist). Neurons and glia developed only in cultures of crest-derived cells, and did so even when endothelin-3 was absent and BQ 788 was present. Endothelin-3 inhibited neuronal development, an effect that was mimicked by IRL 1620 and blocked by BQ 788. Endothelin-3 failed to stimulate the incorporation of [3H]thymidine or bromodeoxyuridine. Smooth muscle development in non-crest-derived cell cultures was promoted by endothelin-3 and inhibited by BQ 788. In contrast, transcription of laminin alpha1, a smooth muscle-derived promoter of neuronal development, was inhibited by endothelin-3, but promoted by BQ 788. Neurons did not develop in explants of the terminal bowel of E12 ls/ls (endothelin-3-deficient) mice, but could be induced to do so by endothelin-3 if a source of neural precursors was present. We suggest that endothelin-3/endothelin B normally prevents the premature differentiation of crest-derived precursors migrating to and within the fetal bowel, enabling the precursor population to persist long enough to finish colonizing the bowel.  (+info)

Binding of the G domains of laminin alpha1 and alpha2 chains and perlecan to heparin, sulfatides, alpha-dystroglycan and several extracellular matrix proteins. (2/4299)

The C-terminal G domain of the mouse laminin alpha2 chain consists of five lamin-type G domain (LG) modules (alpha2LG1 to alpha2LG5) and was obtained as several recombinant fragments, corresponding to either individual modules or the tandem arrays alpha2LG1-3 and alpha2LG4-5. These fragments were compared with similar modules from the laminin alpha1 chain and from the C-terminal region of perlecan (PGV) in several binding studies. Major heparin-binding sites were located on the two tandem fragments and the individual alpha2LG1, alpha2LG3 and alpha2LG5 modules. The binding epitope on alpha2LG5 could be localized to a cluster of lysines by site-directed mutagenesis. In the alpha1 chain, however, strong heparin binding was found on alpha1LG4 and not on alpha1LG5. Binding to sulfatides correlated to heparin binding in most but not all cases. Fragments alpha2LG1-3 and alpha2LG4-5 also bound to fibulin-1, fibulin-2 and nidogen-2 with Kd = 13-150 nM. Both tandem fragments, but not the individual modules, bound strongly to alpha-dystroglycan and this interaction was abolished by EDTA but not by high concentrations of heparin and NaCl. The binding of perlecan fragment PGV to alpha-dystroglycan was even stronger and was also not sensitive to heparin. This demonstrated similar binding repertoires for the LG modules of three basement membrane proteins involved in cell-matrix interactions and supramolecular assembly.  (+info)

Expression of extracellular matrix proteins in cervical squamous cell carcinoma--a clinicopathological study. (3/4299)

AIM: To evaluate the intracellular and peritumoral expression of matrix proteins in squamous cell carcinoma of the uterine cervix using immunohistochemistry. METHODS: 71 squamous cell carcinomas and 10 controls were stained for laminin, fibronectin, and collagen IV. Cytoplasmic staining in tumour cells and peritumoral deposition of matrix proteins were evaluated. The association between staining results and patient age, tumour stage, histological grade, and survival was studied. RESULTS: Positive cytoplasmic staining for laminin, fibronectin, and collagen IV was observed in 17 (23.9%), 27 (38%), and 10 (14.1%) cases, respectively. Staining for laminin was most pronounced in the invasive front of tumour islands, while for fibronectin and collagen IV it appeared to be diffuse. Peritumoral staining for laminin and collagen IV was detected in 12 cases (16.9%). Early stage (Ia1-Ia2) tumours were uniformly negative for all three proteins. Cytoplasmic staining for laminin correlated with positive staining for fibronectin and collagen IV, and with the presence of a peritumoral deposition of collagen IV and laminin. There was no correlation with any of the three markers between staining results and patient age, stage, grade, or survival. CONCLUSIONS: Expression of extracellular matrix proteins in some cervical squamous cell carcinomas might reflect the enhanced ability of these tumours to modify the peritumoral stroma. This ability seems to be absent in early stage tumours. The correlation between intracytoplasmic and peritumoral expression of matrix proteins supports the evidence of their synthesis by tumour cells. However, this property did not correlate with disease outcome in this study.  (+info)

alphaSU2, an epithelial integrin that binds laminin in the sea urchin embryo. (4/4299)

At gastrulation in the sea urchin embryo dramatic cell adhesion changes contribute to primary mesenchyme cell ingression movements and to cell rearrangements during archenteron invagination. At ingression, quantitative adhesion assays demonstrated previously that primary mesenchyme cells (PMCs) change their affinity for neighboring cells, for a fibronectin-like substrate, and for the hyaline layer. To investigate the molecular basis for these and other differential cell affinities at gastrulation, we have identified an integrin that appears to be responsible for specific alterations in cell-substrate adhesion to laminin. During early cleavage stages blastomeres adhere poorly to laminin substrates. Around hatching there is a large increase in the ability of blastomeres to bind to laminin and this increase correlates temporally with the expression of an integrin on the basal surface all blastomeres. PMCs, after undergoing their epithelial-mesenchymal transition, have a strongly reduced affinity for laminin relative to ectoderm cells and, correspondingly, do not stain for the presence of the integrin. We identified the alpha integrin cDNA from Lytechinus variegatus by RT-PCR. Overlapping clones were obtained from a midgastrula cDNA library to provide a complete sequence for the integrin. The composite cDNA encoded a protein that was most similar to the alpha5 subgroup of vertebrate integrins, but there was not a definitive vertebrate integrin homolog. Northern blots and Western immunoblots showed that the sea urchin integrin, which we have named alphaSU2, is present in eggs and during all stages of development. Immunolocalization with specific polyclonal antibodies showed that alphaSU2 first appears on the basal cell surface of epithelia at the midblastula stage, at a time correlating with the increase in adhesive affinity for laminin. The protein remains at high levels on the basal surface of ectoderm cells but is temporarily reduced or eliminated from endoderm cells during their convergent-extension movements. To confirm integrin binding specificity, alphaSU2 was transfected into an alpha-integrin-deficient CHO cell line. alphaSU2-expressing CHO cells bound well to isolated sea urchin basal lamina and to purified laminin. The transfected cells bound weakly or not at all to fibronectin, type I collagen, and type IV collagen. This is consistent with the hypothesis that alphaSU2 integrin functions by binding epithelial cells to laminin in the basal lamina. In vivo, modulation of alphaSU2 integrin expression correlates with critical adhesive changes during cleavage and gastrulation. Thus, this protein appears to be an important contributor to the morphogenetic rearrangements that characterize gastrulation in the sea urchin embryo.  (+info)

The role of cell adhesion molecules in Drosophila heart morphogenesis: faint sausage, shotgun/DE-cadherin, and laminin A are required for discrete stages in heart development. (5/4299)

Heart development in the Drosophila embryo starts with the specification of cardiac precursors from the dorsal edge of the mesoderm through signaling from the epidermis. Cardioblasts then become aligned in a single row of cells that migrate dorsally. After contacting their contralateral counterparts, cardioblasts undergo a cytoskeletal rearrangement and form a lumen. Its simple architecture and cellular composition makes the heart a good system to study mesodermal patterning, intergerm layer signaling, and the function of cell adhesion molecules (CAMs) during morphogenesis. In this paper we focus on three adhesion molecules, faint sausage (fas), shotgun/DE-cadherin (shg/DE-Cad), and laminin A (lam A), that are essential for heart development. fas encodes an Ig-like CAM and is required for the correct number of cardioblasts to become specified, as well as proper alignment of cardioblasts. shg/DE-Cad is expressed and required at a later stage than fas; in embryos lacking this gene, cardioblasts are specified normally and become aligned, but do not form a lumen. Additionally, cardioblasts of shg mutant embryos show a redistribution of phosphotyrosine as well as a loss of Armadillo from the membrane, indicating defects in cell polarity. The shg phenotype could be phenocopied by applying EGTA or cytochalasin D, supporting the view that Ca2+-dependent adhesion and the actin cytoskeleton are instrumental for heart lumen formation. As opposed to cell-cell adhesion, cell-substrate adhesion mechanisms are not required for heart morphogenesis, but only for maintenance of the differentiated heart. Embryos lacking the lam A gene initially developed a normal heart, but showed twists and breaks of cardioblasts at late embryonic stages. We discuss our findings in light of recent results that elucidate the function of different adhesion systems in vertebrate heart development.  (+info)

Cell adhesion activity for murine carcinoma cells of a wheat germ 55-kDa protein with binding affinity for animal extracellular matrix proteins. (6/4299)

A wheat germ 55-kDa protein was isolated by affinity chromatography with Matrigel immobilized on agarose, followed by preparative gel electrophoresis. This Matrigel-binding protein designated as WG-55 had an amino-terminal amino acid sequence which is identical to that of a putative mature form of wheat storage protein Gbl 1. WG-55 reacted with concanavalin A, indicating its glycoprotein nature as expected from the amino acid sequence of Gbl 1. As expected, similarly, WG-55 exhibited RGD-dependent cell adhesion activity for murine carcinoma cells. These data suggest that WG-55 or mature Gbl 1 protein may play a role in plant cell adhesion.  (+info)

Development of cytotrophoblast columns from explanted first-trimester human placental villi: role of fibronectin and integrin alpha5beta1. (7/4299)

Human first-trimester floating mesenchymal villi explanted onto gels of collagen I or Matrigel were observed to undergo de novo development of anchoring sites. These consisted of cytotrophoblast columns that formed by proliferation of stem villous cytotrophoblast cells, as revealed by whole-mount and thin-section microscopy and incorporation of bromodeoxyuridine into DNA. Column formation occurred exclusively at the distal tips of the villi. No column formation was observed in tissue explanted onto agarose. On Matrigel, the developing columns penetrated downwards into the matrix, whereas on collagen I, cytotrophoblast sheets spread across the surface of the gel and merged to form a shell. The developing columnar cytotrophoblast up-regulated integrins alpha1beta1 and alpha5beta1 and produced an extracellular matrix containing oncofetal fibronectin, as in vivo. Function-blocking antibodies were used to investigate the role of the integrin-fibronectin interaction in anchoring villus development on collagen I. Antibodies to fibronectin and the integrin subunits alpha5 and beta1, added at 24 h, all changed the pattern of cytotrophoblast outgrowth. Anti-fibronectin caused cell rounding within the cytotrophoblast sheet and increased the population of single cells at its periphery. Anti-integrin alpha5 caused rounding and redistribution of cells within the outgrowth. In the presence of anti-integrin beta1, cell-collagen interactions within the sheet were destabilized, often leading to the appearance of an annulus of aggregated cells at the periphery. These results show that 1) mesenchymal villi retain the potential to form anchoring sites until at least the end of the first trimester, 2) adhesion to a permissive extracellular matrix stimulates cytotrophoblast proliferation and differentiation along the extravillous lineage, 3) integrin alpha5beta1-fibronectin interactions contribute significantly to anchorage of the placenta to uterine extracellular matrix. We suggest that as the developing placenta ramifies, new sites of anchorage form whenever peripheral villi contact decidua. This process is predicted to contribute to the stability of the placental-decidual interface.  (+info)

Extracellular matrix regulates apoptosis in mammary epithelium through a control on insulin signaling. (8/4299)

Adherent epithelial cells require interactions with the extracellular matrix for their survival, though the mechanism is ill-defined. In long term cultures of primary mammary epithelial cells, a laminin-rich basement membrane (BM) but not collagen I suppresses apoptosis, indicating that adhesion survival signals are specific in their response (. J. Cell Sci. 109:631-642). We now demonstrate that the signal from BM is mediated by integrins and requires both the alpha6 and beta1 subunits. In addition, a hormonal signal from insulin or insulin-like growth factors, but not hydrocortisone or prolactin, is necessary to suppress mammary cell apoptosis, indicating that BM and soluble factors cooperate in survival signaling. Insulin induced autophosphorylation of its receptor whether mammary cells were cultured on collagen I or BM substrata. However, both the tyrosine phosphorylation of insulin receptor substrate-1 and its association with phosphatidylinositol 3-kinase were enhanced in cells cultured on BM, as was the phosphorylation of the phosphatidylinositol 3-kinase effector, protein kinase B. These results suggest a novel extracellular matrix-dependent restriction point in insulin signaling in mammary epithelial cells. The proximal signal transduction event of insulin receptor phosphorylation is not dependent on extracellular matrix, but the activation of downstream effectors requires adhesion to BM. Since phosphatidylinositol 3-kinase was required for mammary epithelial cell survival, we propose that a possible mechanism for BM-mediated suppression of apoptosis is through its facilitative effects on insulin signaling.  (+info)

TY - JOUR. T1 - Galectin-3, a laminin binding protein, fails to modulate adhesion of human melanoma cells to laminin. AU - Van Den Brule, F. A.. AU - Buicu, C.. AU - Sobel, M. E.. AU - Liu, Fu-Tong. AU - Castronovo, V.. PY - 1995. Y1 - 1995. N2 - Galectin-3 is a laminin binding protein which expression is altered in a variety of human carcinomas including colon, breast and endometrium. In these tumors, we consistently observed a down regulation of galectin-3 expression related to increased aggressiveness. Galectin-3 belongs to a family of galactose-binding lectins and binds laminin through its numerous poly-N-acetyllactosamine chains. To date, the exact role of galectin-3 in the complex interactions between cancer cells and laminin has not been clearly defined. Adhesion of melanoma cells to laminin is a critical event during tumor invasion and metastasis. In this study, we explore the possibility that galectin-3 could modulate attachment of two human melanoma cell lines to laminin. A2058 and ...
TY - JOUR. T1 - Laminin α subunits and their role in C. elegans development. AU - Huang, Cheng Chen. AU - Hall, David H.. AU - Hedgecock, Edward M.. AU - Kao, Gautam. AU - Karantza, Vassiliki. AU - Vogel, Bruce E.. AU - Hutter, Harald. AU - Chisholm, Andrew D.. AU - Yurchenco, Peter D.. AU - Wadsworth, William G.. PY - 2003/7/1. Y1 - 2003/7/1. N2 - Laminins are heterotrimeric (α/β/γ) glycoproteins that form a major polymer within basement membranes. Different α, β and γ subunits can assemble into various laminin isoforms that have different, but often overlapping, distributions and functions. In this study, we examine the contributions of the laminin α subunits to the development of C. elegans. There are two α, one β and one γ laminin subunit, suggesting two laminin isoforms that differ by their α subunit assemble in C. elegans. We find that near the end of gastrulation and before other basement membrane components are detected, the α subunits are secreted between primary tissue ...
|strong|Mouse anti Human laminin alpha 2 antibody, clone 5H2|/strong| recognizes the ~80 kDa fragment of laminin subunit alpha-2, also known as the laminin M chain, laminin-12 subunit alpha, lami…
The basal lamina that ensheaths skeletal muscle fibers traverses the synaptic cleft at the neuromuscular junction. Synaptic and extrasynaptic portions of the basal lamina contain different laminin beta chains: beta 2 (or s) at synapses and beta 1 (or B1) extrasynaptically. Laminin beta 2 is also confined to synapselike patches on myotube surfaces in vitro, whereas beta 1 is present throughout the extracellular matrix. This differential localization of laminin beta chains was analyzed by expression of chimeric beta 1-beta 2 molecules in cultured mouse myotubes. A 16-amino acid carboxyl-terminal sequence in beta 2 was necessary for synaptic localization, and an amino-terminal domain in beta 1 promoted association with extracellular fibrils. The synaptic targeting sequence of beta 2 contains a site previously shown to be adhesive for motor neurons. ...
Laminin alpha2 deficiency presents at birth with muscle weakness, hypotonia, and usually asymptomatic white matter signal on MRI. Few patients with laminin alpha2 deficiency have been described with seizures and structural brain abnormalities. The re
Transfection of a wild-type laminin γ2 cDNA in LSV5 keratinocytes rescues expression of functional laminin 5 molecules (Gagnoux-Palacios et al. 1996). Similar to laminin 5 produced by wild-type keratinocytes, the recombinant laminin 5 secreted by the reverted LSV5 cells undergoes extracellular maturation, including proteolytic excision of the NH2-terminal domains of the γ2 short arm. In this study, we examined the functional role of the laminin γ2 short arm by complementing the genetic defect of LSV5 cells by transfer of mutant γ2 cDNAs. Expression of the mutant cDNAs demonstrated that all the different recombinant γ2 chains associate with the endogenous laminin α3 and β3 polypeptides to assemble into α3β3γ2 heterotrimers, and that the NH2-terminal domains of the γ2 chain are required for deposition and incorporation of laminin 5 in the ECM.. Our results, for the first time, define a physiologic function for the short arm of the γ2 chain. We demonstrate that mutant laminin 5 ...
To study genes that may be crucial for the male germ cell development of Drosophila we screened a cDNA expression library with a polyclonal antiserum against testis proteins of Drosophila hydei. We identified a cDNA fragment that exhibited a complete sequence similarity with the cDNA of the laminin B2 chain, an important component of the extracellular matrix. Transcripts of laminin B2 were detected in the RNA of male germ cells with the polymerase chain reaction and by in situ hybridization. We studied the reaction of different polyclonal antibodies including those against a Drosophila laminin B2-lac fusion protein, the entire Drosophila laminin complex, or against the mouse laminin complex and against laminin A and B1 chains with specific structures in developing male germ cells of Drosophila. Antigenic sites against laminin B2 were found in the lampbrush loops in primary spermatocyte nuclei, in nuclei of spermatids, and in heads of spermatozoa. The axonemes of elongating spermatids react with ...
Precise contact between epithelial cells and their underlying basement membrane is crucial to the maintenance of tissue architecture and function. To understand the role that the laminin receptor dystroglycan (DG) plays in these processes, we assayed cell responses to laminin-111 following conditional ablation of DG gene (Dag1) expression in cultured mammary epithelial cells. Strikingly, DG loss disrupted laminin-111-induced polarity and beta-casein production, and abolished laminin assembly at the step of laminin binding to the cell surface. Dystroglycan re-expression restored these deficiencies. Investigations of the mechanism revealed that DG cytoplasmic sequences were not necessary for laminin assembly and signaling, and only when the entire mucin domain of extracellular DG was deleted did laminin assembly not occur. These results demonstrate that DG is essential as a laminin-111 co-receptor in mammary epithelial cells that functions by mediating laminin anchoring to the cell surface, a process that
TY - JOUR. T1 - Mild clinical phenotype in a 12-year-old boy with partial merosin deficiency and central and peripheral nervous system abnormalities. AU - Mora, Marina. AU - Moroni, Isabella. AU - Uziel, Graziella. AU - Di Blasi, Claudia. AU - Barresi, Rita. AU - Farina, Laura. AU - Morandi, Lucia. PY - 1996/10. Y1 - 1996/10. N2 - We found partial merosin deficiency in a boy presenting at 12 yr with marked limb weakness and a waddling gait. Magnetic resonance imaging (MRI) showed the characteristic white matter abnormalities of merosin-negative congenital muscular dystrophy. There were also peripheral demyelinating polyneuropathy and evoked potential abnormalities. Unlike classic merosin-negative congenital muscular dystrophy, however, our patient was less hypotonic and weak and was able to achieve independent walking. Both by immunohistochemistry and Western blot merosin was shown to be moderately reduced. By immunostaining the α1 laminin chain was overexpressed and the β1 laminin chain was ...
The ability of thioglycollate (TG)-elicited mouse peritoneal macrophages to adhere to a laminin substratum has been studied. These cells do not adhere to laminin-coated (20 micrograms/ml) surfaces, but the addition of phorbol myristate acetate (PMA; 50 ng/ml) results in their rapid adherence and spreading on this substratum. TG-elicited and PMA-activated macrophages, however, can bind soluble laminin. Macrophages adhere to fibronectin-coated surfaces and tissue culture plastic without PMA stimulation, and PMA does not increase the number of cells that adhere to these surfaces. The predominant surface proteins that bind specifically to laminin-Sepharose exhibit an Mr of 67 and 36 kD, but the expression of these proteins does not increase after PMA stimulation. Laminin receptor antibodies immunoprecipitate the 67-kD protein from radiolabled surface lysates and are capable of blocking macrophage adherence to a laminin substratum. Indirect immunofluorescence microscopy indicates that PMA stimulation does
Laminins, a family of extracellular matrix glycoproteins, are the major noncollagenous constituent of basement membranes. They have been implicated in a wide variety of biological processes including cell adhesion, differentiation, migration, signaling, neurite outgrowth and metastasis. Laminins are composed of 3 non identical chains: laminin alpha, beta and gamma (formerly A, B1, and B2, respectively) and they form a cruciform structure consisting of 3 short arms, each formed by a different chain, and a long arm composed of all 3 chains. Each laminin chain is a multidomain protein encoded by a distinct gene. Several isoforms of each chain have been described. Different alpha, beta and gamma chain isomers combine to give rise to different heterotrimeric laminin isoforms which are designated by Arabic numerals in the order of their discovery, i.e. alpha1beta1gamma1 heterotrimer is laminin 1. The biological functions of the different chains and trimer molecules are largely unknown, but some of the ...
TY - JOUR. T1 - Structural analysis of a human glial variant laminin. AU - LeMosy, Ellen. AU - Lightner, Virginia A.. AU - Erickson, Harold P.. PY - 1996/8/25. Y1 - 1996/8/25. N2 - Astrocytes secrete laminin-like molecules in culture and may represent a major source of laminin in the developing central nervous system, yet these laminins have not been extensively characterized. We previously reported the presence of an astrocyte-derived variant laminin in media conditioned by human U251 MG astrocytoma cells. This laminin was partially purified in a highly anionic Mono Q fraction with strong adhesion activity for fibroblasts and glial cells (Aukhil et al. (1990) Matrix 10: 98-111). We now show that glial laminin could be dissociated from an anionic species, perhaps an ~400- kDa keratan sulfate proteoglycan present in the preparation, by a second round of Mono Q anion exchange chromatography in the presence of 6 M urea. Cell adhesion activity remained tightly associated with laminin-containing ...
FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes one of the vertebrate laminin alpha chains. Laminins, a family of extracellular matrix glycoproteins, are the major noncollagenous constituent of basement membranes. They have been implicated in a wide variety of biological processes including cell adhesion, differentiation, migration, signaling, neurite outgrowth and metastasis. Laminins are composed of 3 non identical chains: laminin alpha, beta and gamma (formerly A, B1, and B2, respectively) and they form a cruciform structure consisting of 3 short arms, each formed by a different chain, and a long arm composed of all 3 chains. Each laminin chain is a multidomain protein encoded by a distinct gene. The protein encoded by this gene is the alpha-5 subunit of of laminin-10 (laminin-511), laminin-11 (laminin-521) and laminin-15 (laminin-523). [provided by RefSeq, Jun 2013 ...
Laminin S/Laminin beta 2 Proteins available through Novus Biologicals. Browse our Laminin S/Laminin beta 2 Protein catalog backed by our Guarantee+.
Laminin S/Laminin beta 2 Proteins available through Novus Biologicals. Browse our Laminin S/Laminin beta 2 Protein catalog backed by our Guarantee+.
BACKGROUND: LDL oxidation in the vascular wall is associated with aldehyde modification of surrounding extracellular matrix proteins that may target autoimmune responses against vascular tissues. Here we investigated the possible influence of immunity against a malondialdehyde (MDA)-modified form of the basement membrane protein laminin on atherosclerosis. METHODS AND RESULTS: IgM and IgG autoantibodies were present in human plasma and a prospective clinical study demonstrated that individuals who later suffered from acute cardiovascular events had lower levels of MDA-laminin antibodies compared to those in the control group. Immunohistochemical analysis of atherosclerotic plaques from Apoe(-/-) mice demonstrated co-localization between laminin and MDA epitopes, however MDA-laminin IgG was absent in mouse plasma. To determine the effect of MDA-laminin immunity, Apoe(-/-) mice were immunized with MDA-laminin. Analysis of circulating leukocytes at 12 weeks demonstrated increased T-cell activation, ...
Airway smooth muscle (ASM) contraction underlies acute bronchospasm in asthma. ASM cells can switch between a synthetic-proliferative phenotype and a contractile phenotype. While the effects of extracellular matrix (ECM) components on modulation of ASM cells to a synthetic phenotype have been reported, the role of ECM components on maturation of ASM cells to a contractile phenotype in adult lung is unclear. As both changes in ECM components and accumulation of contractile ASM are features of airway wall remodelling in asthma, we examined the role of the ECM protein, laminin, in the maturation of contractile phenotype in human ASM cells. Human ASM cells were made senescence-resistant by stable expression of human telomerase reverse transcriptase. Maturation to a contractile phenotype was induced by 7-day serum deprivation, as assessed by immunoblotting for desmin and calponin. The role of laminin on ASM maturation was investigated by comparing the effects of exogenous laminin coated on culture plates,
Airway smooth muscle (ASM) contraction underlies acute bronchospasm in asthma. ASM cells can switch between a synthetic-proliferative phenotype and a contractile phenotype. While the effects of extracellular matrix (ECM) components on modulation of ASM cells to a synthetic phenotype have been reported, the role of ECM components on maturation of ASM cells to a contractile phenotype in adult lung is unclear. As both changes in ECM components and accumulation of contractile ASM are features of airway wall remodelling in asthma, we examined the role of the ECM protein, laminin, in the maturation of contractile phenotype in human ASM cells. Human ASM cells were made senescence-resistant by stable expression of human telomerase reverse transcriptase. Maturation to a contractile phenotype was induced by 7-day serum deprivation, as assessed by immunoblotting for desmin and calponin. The role of laminin on ASM maturation was investigated by comparing the effects of exogenous laminin coated on culture plates,
Laminins are continuously expressed during all developing stages of cartilage and cartilage-like tissues and show a region-specific expression pattern. In cartilage from newborn mice, basement membrane components are widespread in the territorial and interterritorial matrix, while in mature cartilage of adult mice the basement membrane components are localized mainly to a narrow pericellular zone around the chondrocytes (Kvist, 2008). Of the laminin subunits surveyed, α1, α5, β1, and γ1 have shown the highest staining intensity (Kvist, 2008). Lee and colleagues found that laminin chains (α1, α2, β1, β2, and γ1), produced by chicken embryo sternal chondrocytes, exhibited an increased expression in aggregated cells during the maturation stage (Lee, 1997). In newborn mouse knee cartilage, laminin 111 showed staining in the extracellular matrix, while laminin α5 (511 and 521) stained weakly at this point (Kvist, 2008). The laminin β1 chain is expressed from gestational weeks (gw) 10 ...
After the game, we had FCA, its the bomb, and Mr. Sheek talked about Laminin. Laminin by definition is the basement membrane, which is a protein network foundation for most cells and organs. Now youre wondering why in the world you should keep reading this, and why i am telling you this. Well Im here to tell you that Laminin is what our bodies is made up of. Scripture says that God is in us. And people wonder how in the world can God be In us. Well Laminin is proof. Laminin is, well let me show you what Laminin is. ...
Corning™ rLaminin-521 (Human) is an animal-component-free recombinant heterotrimer composed of a5, B2, and y1 chains expressed in a mammalian cell culture system. The Corning rLaminin-521 (Human) surface supports such key benefits as ROCK inhibitor independent, single-cell expansion of PSCs and inhibition of spontaneous differentiation, improving hPSC culture ease and efficiency.. A collaboration focused on bringing together better solutions for stem cell research. Corning has partnered with BioLamina for the supply of recombinant human laminin 521 (rLaminin-521), a next generation surface that enables feeder-free, single cell passaging of pluripotent stem cells (PSCs) without ROCK-inhibitor. Now weve gone one step further and are working together to combine BioLaminas expertise in recombinant laminin expression with Cornings extensive line of vessels and proprietary surface coating technologies. This partnership brings robust and scalable expansion solutions to the emerging stem cell ...
Corning has partnered with BioLamina for the supply of recombinant human laminin 521 (rLaminin-521), a next generation surface that enables feeder-free, single cell passaging of pluripotent stem cells (PSCs) without ROCK-inhibitor. Now weve gone one step further and are working together to combine BioLaminas expertise in recombinant laminin expression with Cornings extensive line of vessels and proprietary surface coating technologies. This partnership brings robust and scalable expansion solutions to the emerging stem cell processing market, enabling our customers to scale-up effectively and mitigate risk.. Now backed by Cornings large network, worldwide distribution channels, and cell culture portfolio, researchers globally have better access and availability to rLaminin-521 (Human) than ever before. rLaminin-521 (Human) joins the Corning ECM mimetic and advanced surfaces family: animal component-free surfaces suited for stem and progenitor cell expansion under cGMP processing environments. ...
Z3461763 (talk) 16:15, 16 April 2015 (EST) Ill upload mine when I get home because its saved on my usb... Its completed just havent uploaded. :D --Z3461763 (talk) 21:16, 16 April 2015 (EST) is there a reason why anything im trying to upload wont upload? its been an hour and nothing will save! --Z3461763 (talk) 23:16, 16 April 2015 (EST) The Heterotrimeric Laminin Coiled - Coil Domain Exerts Anti - Adhesive Effects and Induces a Pro - Invasive Phenotype Laminins are a family of ECM glycoproteins localised in the basement membrane. As well as their structural roles, they also regulate cellular processes. These include cell migration, differentiation and proliferation. Laminins also have multiple binding partners; hence, the study was to determine what would happen to individual truncated Laminin chains in the absence of their normal partners. They did this by selecting a transfectable cell line with low endogenous Laminin chain expression, by first encoding the truncated Laminin α1, β1 ...
Z3461763 (talk) 16:15, 16 April 2015 (EST) Ill upload mine when I get home because its saved on my usb... Its completed just havent uploaded. :D --Z3461763 (talk) 21:16, 16 April 2015 (EST) is there a reason why anything im trying to upload wont upload? its been an hour and nothing will save! --Z3461763 (talk) 23:16, 16 April 2015 (EST) The Heterotrimeric Laminin Coiled - Coil Domain Exerts Anti - Adhesive Effects and Induces a Pro - Invasive Phenotype Laminins are a family of ECM glycoproteins localised in the basement membrane. As well as their structural roles, they also regulate cellular processes. These include cell migration, differentiation and proliferation. Laminins also have multiple binding partners; hence, the study was to determine what would happen to individual truncated Laminin chains in the absence of their normal partners. They did this by selecting a transfectable cell line with low endogenous Laminin chain expression, by first encoding the truncated Laminin α1, β1 ...
Genetic variation in a member of the laminin gene family affects variation in body composition in Drosophila and humans. . Biblioteca virtual para leer y descargar libros, documentos, trabajos y tesis universitarias en PDF. Material universiario, documentación y tareas realizadas por universitarios en nuestra biblioteca. Para descargar gratis y para leer online.
Laminins, a family of extracellular matrix glycoproteins, are the major noncollagenous constituent of basement membranes. They have been implicated in a wide variety of biological processes including cell adhesion, differentiation, migration, signaling, neurite outgrowth and metastasis. Laminins are composed of 3 non identical chains: laminin alpha, beta and gamma (formerly A, B1, and B2, respectively) and they form a cruciform structure consisting of 3 short arms, each formed by a different chain, and a long arm composed of all 3 chains. Each laminin chain is a multidomain protein encoded by a distinct gene. Several isoforms of each chain have been described. Different alpha, beta and gamma chain isomers combine to give rise to different heterotrimeric laminin isoforms which are designated by Arabic numerals in the order of their discovery, i.e. alpha1beta1gamma1 heterotrimer is laminin 1. The biological functions of the different chains and trimer molecules are largely unknown, but some of the ...
FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] Laminins, a family of extracellular matrix glycoproteins, are the major noncollagenous constituent of basement membranes. They have been implicated in a wide variety of biological processes including cell adhesion, differentiation, migration, signaling, neurite outgrowth and metastasis. Laminins are composed of 3 non identical chains: laminin alpha, beta and gamma (formerly A, B1, and B2, respectively) and they form a cruciform structure consisting of 3 short arms, each formed by a different chain, and a long arm composed of all 3 chains. Each laminin chain is a multidomain protein encoded by a distinct gene. Several isoforms of each chain have been described. Different alpha, beta and gamma chain isomers combine to give rise to different heterotrimeric laminin isoforms which are designated by Arabic numerals in the order of their discovery, i.e. alpha1beta1gamma1 heterotrimer is laminin 1. The ...
Monoclonal clone# G2 antibody for LAMA1; LAMA2; LAMB1; LAMB2 detection. Host: Mouse.Size: 100μg/vial. Tested applications: IHC-P. Reactive species: Feline. LAMA1; LAMA2; LAMB1; LAMB2 information: Molecular Weight: 196474 MW; Subcellular Localization: Sec
The AlphaLISA® immunoassay kit for human laminin enables the detection and quantitation of human laminin in human serum, plasma, lysates, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps). The assay shows no cross-reactivity with human IL-28A, IL-29, and Mouse Laminin.
In this work we analyzed data obtained from case-history of 36 patients operated in Hospital for tumors, Zagreb in period 2006 till 2007 year. Ductal breast cancer in situ (DCIS) were diagnosed in 24 patients while in 12 patients diagnosis was ductal micro invasive breast cancer. Aim of thesis was to define expression of polyclonal and monoclonal anti-laminin antibodies on basement membrane of ducts in breast cancer using immunohistochemical method. We investigated whether loss of laminin in basement membrane can be a predictive marker for micro invasiveness. We also investigated association between prognostic factors as age of patients, tumor differentiation stage, steroid hormone receptors and HER2 status with anti-laminin expression and micro invasion appearance. Average age of patients was 56.5 years. The results showed that polyclonal anti-laminin is an important predictive factor of micro invasion appearance in DCIS (p=0,00026) comparing to monoclonal anti-laminin (p=0,23765) (p,0,05 ). ...
Laminins are glycoproteins that are major constituents of the basement membrane of cells. Laminins are trimeric molecules; laminin-1 is an alpha1 beta1 gamma1 trimer. It has been suggested that the domains I and II from laminin A, B1 and B2 may come together to form a triple helical coiled-coil structure [(PUBMED:3182802)]. Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organisation of cells into tissues during embryonic development by interacting with other extracellular matrix components.. ...
Laminin is a large molecular weight glycoprotein present only in basement membranes in almost every animal tissue. Each laminin is a heterotrimer assembled from α, β and γ chain subunits, secreted and incorporated into cell-associated extracellular matrices. The laminins can self-assemble, bind to other matrix macromolecules, and have unique and shared cell interactions mediated by integrins, dystroglycan, and other receptors. Through these interactions, laminins critically contribute to cell differentiation, shape and movement, maintenance of tissue phenotypes, and promotion of tissue survival [1,2]. The different laminin chains share a 600-residue domain I/II which oligomerizes into a rod-like coiled-coil structure forming the long arm of laminins. The N-terminal short arms consist of rod-like elements (domain III and V) formed by tandem arrays of laminin-type EGF modules (see ,PDOC00961,) and several globular domains: domains IV and domain VI (laminin N-terminal) (see ,PDOC51117,). All α ...
Laminin, an extracellular matrix protein, is a major component of the basement membrane. It is thought to mediate the attachment, migration, and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components. It is composed of three subunits, alpha, beta, and gamma, which are bound to each other by disulfide bonds into a cross-shaped molecule. This gene encodes the alpha 2 chain, which constitutes one of the subunits of laminin 2 (merosin) and laminin 4 (s-merosin). Mutations in this gene have been identified as the cause of congenital merosin-deficient muscular dystrophy. Two transcript variants encoding different proteins have been found for this gene.[3] ...
We use the same antibody at 1:20 with proteinase k from DAKO for 10min at RT. Juan C. Gutierrez, HT(ASCP) Histology Laboratory Supervisor (210)704-2533 My opinions are my own and do not reflect those of my employer. Long live free speech! -----Original Message----- From: [email protected] [mailto:[email protected]] On Behalf Of Coolen, Neeltje Sent: Thursday, April 07, 2005 3:37 AM To: [email protected] Subject: [Histonet] Laminin antibody Dear Histonetters, Are there any people who have experience with the following anti-laminin antibody: mouse monoclonal, clone 4C7 from DAKO (code no: M0638)? We tried to use this antibody on paraffin-embedded human skin tissue (formalin-fixed), but it did not work. We tried several antigen retrieval procedures, like pepsin, proteinase K and trypsin digestion, but all of these fail. Does anyone know an appropriate antigen retrieval or should I try another antibody? Thank you for your help. Neeltje ...
Multidomain proteins of the extracellular matrix (ECM) play an important role in development and maintenance of cellular organization and in tissue repair. Several ECM proteins such as laminin, tenascin and thrombospondin contain domains with homology to epidermal growth factor (EGF) and exhibit growth promoting activity. The mitogenic activity of laminin is restricted to a fragment which consists of about 25 repeating domains with partial homology to EGF and comprises the rod-like inner regions of the three short arms of the four armed molecule. The mitogenic activity does not correlate with promotion of cell attachment and neurite outgrowth for which major functional sites have been found in other regions of the laminin molecule. It is suggested that EGF-like domains in laminin, in other ECM proteins and in the extracellular portions of some membrane proteins are signals for cellular growth and differentiation. Because they are integral parts of large molecules and often of supramolecular ...
Laminin-5 is a trimer of laminin α3, β3 and γ2 chains that is found in the intestinal basement membrane. Deposition of the laminin γ2 chain at the basement membrane is of great interest because it undergoes a developmental shift in its cellular expression. Here we study the regulatory elements that control basal and cytokine-activated transcriptional expression of the LAMC2 gene, which encodes the laminin γ2 chain. By using transient transfection experiments we demonstrated the presence of constitutive and cytokine-responsive cis-elements. Comparison of the transcriptional activity of the LAMC2 promoter in the epithelial HT29mtx cells with that in small-intestinal fibroblastic cells (C20 cells) led us to conclude that two regions with constitutive epithelium-specific activity are present between positions -1.2 and -0.12 kb. This was further validated by transfections of primary foetal intestinal endoderm and mesenchyme. A 2.5 kb portion of the LAMC2 5ʹ flanking region was equally ...
Description: A polyclonal antibody for detection of Laminin Alpha-3 from Human, Mouse, Rat. This Laminin Alpha-3 antibody is for IHC-P, IF, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the C-terminal region of human Laminin ?-3 at AA rangle: 2540- ...
Laminin beta 1兔多克隆抗体(ab69633)可与人样本反应并经WB, ELISA, IHC, ICC/IF实验严格验证。所有产品均提供质保服务,中国75%以上现货。
Laminin is a major component of the extracellular matrix (ECM) and arose early in animal evolution. Due to its role in cell adhesion and migration, laminin is central to development and disease. Laminin is a heterotrimer with a total mass of up to 800 kD. Its three subunits (α, β, and γ) assemble into a distinctive cross-shaped structure, copies of which further self-assemble to form a network. Higher organisms contain a number of isotypes of each subunit, which produce, combinatorially, a rich family of related proteins with certain structural and functional distinctions. Many fundamental aspects of laminin structure are understood from rotary shadowing electron microscopy, recognizable amino acid sequence motifs (i.e., coiled-coil heptad repeats), and X-ray crystallography of laminin fragments. However, other conserved portions of laminin, namely the L4, LF, and Lβ knob domains, are still poorly understood, both structurally and functionally. The L4 and LF domains are ...
Use Corning Laminin surfaces as a thin coating on tissue culture surfaces or as a soluble additive to culture medium to promote cell adhesion, migration, chemotaxis, growth, and differentiation. Laminin/Entactin Complex High Concentration (HC) is a special formulation that Corning has developed to enhance three-dimensional (3D) cell culture applications. Culturing cells in or on gels such as Laminin/Entactin Complex enable the study of specific mechanisms that dictate cell differentiation and functionality.. Applications include:. ...
Laminin大鼠单克隆抗体[MEC5](ab2466)可与小鼠, 大鼠, 人样本反应并经IHC实验严格验证,Abcam Laminin抗体被2篇文献引用并得到1个独立的用户反馈。所有产品均提供质保服务,中国75%以上现货。
Am. J. Respir. Cell Mol. BioI. 1996. Here, the authors studied the expression of different laminin chains and their integrin receptors in fetal and adult lung by immunohistochemistry. Special attention was focused on the changes in the expression of these proteins during the development from the pseudoglandular (PG) and canalicular stages to adult lung and on the possible implications of the changes for the normal lung development. The most significant changes in the expression pattern were found during the development from the PO stage to the canalicular stage. Basement membranes (BM) of both the epithelial buds and the becoming bronchi showed reactivity for laminin α1, a3, and B3 chains at all stages. The a2 chain was expressed only in the epithelial buds at the PO stage, and could not be found in any epithelial structures at the canalicular stage. Similarly, at the PG stage the B2 chain was expressed in BMs of both epithelial buds and bronchi but disappeared from the bronchial BM before the ...
Recent studies, on cells cultured in 3D collagen gels, have shown that, beside from their well known biochemical role, fibronectin (FN) and laminin (LM) affect cell functions via a modification of mec
Laminin gamma 1 antibody, Internal (laminin subunit gamma 1) for WB. Anti-Laminin gamma 1 pAb (GTX44865) is tested in Human samples. 100% Ab-Assurance.
Laminin alpha 4 antibody [C3], C-term (laminin, alpha 4) for IHC-P. Anti-Laminin alpha 4 pAb (GTX106134) is tested in Mouse samples. 100% Ab-Assurance.
Laminin SIKVAV peptide induction of monocyte/macrophage prostaglandin E2 and matrix metalloproteinases.: The laminin-derived synthetic peptide containing the SI
These structures contain the extracellular portions of collagen XVII (BP180) and alpha-6-beta-4 (α-6-β-4) integrin. In addition, anchoring filaments contain the molecules laminin 5 and laminin 6. Similar to all members of the family of laminin proteins, laminin 5 is a large heterotrimeric molecule, containing α-3, β-3, and g-2 chains. Laminin 5 forms a disulfide-bonded attachment to laminin 6, the other known anchoring filament laminin, which contains α-3, β-1, and g-1 chains. Laminin 5 also forms a strong association with type VII collagen, which serves to connect anchoring filaments with anchoring fibrils ...
Effects of oral administration of sodium nitrite on laminin expression in mice testicular interstitium: an immunohistochemical study, Sara Amini, Mohammad Reza Nikravesh, Mehdi Jal
Humans and mice with deficiency of the alpha2 subunit of the basement membrane protein laminin-2/merosin suffer from merosin-deficient congenital muscular dystrophy (MCMD). We have expressed a human laminin alpha2 chain transgene under the regulation of a muscle-specific creatine kinase promoter in …
in Journal of the National Cancer Institute (1992), 84(15), 1161-9. BACKGROUND: Interactions between cells and the basement membrane glycoprotein laminin are altered during colon cancer progression. Colon carcinoma and normal mucosa cells express a variety of laminin ... [more ▼]. BACKGROUND: Interactions between cells and the basement membrane glycoprotein laminin are altered during colon cancer progression. Colon carcinoma and normal mucosa cells express a variety of laminin-binding proteins, including the 67-kd laminin receptor (67 LR) and a 31-kd human laminin-binding protein (HLBP31) homologous to the 31-kd human IgE-binding protein/galactoside-binding lectin. PURPOSE: To investigate whether various laminin-binding proteins are differentially expressed in human colon carcinoma, we studied messenger RNA (mRNA) levels of the 67 LR and HLBP31 in matched tumor and adjacent normal mucosa samples from a series of 21 patients. METHODS: Total cellular RNA from tumor and normal mucosa was isolated ...
Schwann cell basement membranes do not appear to form in developing tissues until the stage of neuronal ensheathment, and it has been postulated that axonal contact triggers their assembly, in turn affecting cell polarization, ensheathment and myelination ( Clark and Bunge, 1989; Jaakkola et al., 1993). The data presented in this study suggest that Schwann cell basement membrane assembly is a process that first requires the development of cell surface competency for laminin binding, followed by the binding and assembly of laminin. We consider this ECM as a `nascent basement membrane (i.e. a laminin-based ECM with classical ultrastructural morphology of a basement membrane, but still lacking in type IV collagen). The nascent basement membrane is likely to be no more than one laminin molecular layer thick. Upon exposure of the cells to type IV collagen, this component is incorporated into the laminin matrix. In Schwann cells, the laminin binding/assembly step depends in part upon an interaction ...
Korpos et al. (1) claim that limited information exists on the nature of the ECM [extracellular matrix] of the pancreas and, in particular, on the composition of peri-islet capsule, and that they provide the the first comprehensive analysis of the [ECM] composition of peri-islet capsules.. We dispute the strength of these claims. In mice we originally reported in 2008 that a basement membrane (BM) exists around islets, and eight unique components were identified (type IV collagens α1, α2, laminin α2, β1, γ1, nidogen 1, nidogen 2, perlecan) as well as two composite components (laminin Engelbreth-Holm-Swarm, collagen IV) (2). It was also shown that type IV collagens α3, α4, α5, α6 were not present (2). Korpos et al. (1) were able to find a further three unique components (laminin α4, β2, agrin) and one composite component (laminin 322).. In 2008 in humans (3,4), at least four unique components (laminin α1, α5, β1, γ1) were identified and in their Supplementary Figure 1 staining ...
TY - JOUR. T1 - Rodent myoblast interactions with laminin require cell surface glycoconjugates but not laminin glycosyl groups. AU - Kostrominova, Tatiana. AU - Tanzer, M. L.. PY - 1995. Y1 - 1995. N2 - Laminin glycosyl groups are necessary for the spreading of murine melanoma cells which become attached to this glycoprotein. Laminin has been implicated in myogenesis but the potential role of its glycosyl groups in this process has not been examined. In this study we report the effects of the carbohydrate moieties of laminin on myoblast adhesion, spreading, and differentiation. Unglycosylated laminin from tunicamycin-treated cultures of a mouse cell line, M1536 B3, was used in the experiments. Glycosylated laminin from a murine tumor and from cultures of M1563 B3 cells served as controls. Cell binding experiments with C2C12 mouse myoblasts showed that the cells preferred a laminin-coated surface, compared to the uncoated plastic surface (nontissue culture wells). Myoblasts did not distinguish ...
The disulfide bonding patterns in the N-terminal (LN) domains of the basement membrane protein laminin beta 1 have not been investigated so far. We report an in-depth mass spectrometric analysis using offline nano-high-performance liquid chromatography/matrix-assisted laser desorption/ionization time-of-flight/time-of-flight mass spectrometry (nano-HPLC/MALDI-TOF/TOF-MS) for determining the disulfide bond patterns in the LN-domain of recombinant mouse laminin beta 1 chain for the first time. Mass spectra were recorded and the putatively disulfide-linked peptides were subjected to LIFT-TOF/TOF-MS to confirm the disulfide bond. Screening the fragment ion mass spectra of disulfide-linked peptides for characteristic 66-amu patterns (34 u +32 u), arising from symmetric and asymmetric cleavage of disulfide bonds, facilitated their identification. Using various enzymes for proteolytic digestion of a recombinant laminin beta 1 chain N-terminal protein fragment, a linear bonding pattern of the eight ...
The human non-integrin laminin receptor (LamR) is a multifunctional protein which is localised to a number of sub-cellular locations. LamR is a component of the ribosome and has a number of intracellular functions; it also acts as an extracellular receptor for laminin, growth factors, pathogenic microorganisms, prion proteins, toxins and the anticarcinogen epigallocatechin gallate (ECGC). Although LamR is present in most cellular compartments, its overexpression in many types of cancer cells suggests a vital role for LamR in tumor-cell migration and invasion. There are two isoforms of laminin receptor: the monomeric 37-kDa laminin receptor precursor (37LRP) and the mature 67-kDa laminin receptor (67LR). Although the precise molecular nature of 67LR is unclear, accumulating evidence strongly suggest that 37LRP can undergo homo- and/or hetero-dimerization with Galectin-3 (Gal-3) to form mature 67LR. A recent study demonstrated that both homo- and heterodimer LamR forms are present on the cell ...
Laminin, a component of the embryonic sea urchin basal lamina, is recognized by monoclonal antibody BL1 (Mab BL1). Our results demonstrate that laminin is secreted into the blastcoel at the early blastula stage at a time when the blastomeres undergo a cell shape change and are organized into an epithelium. Laminin is present on the basal surfaces of ectodermal cells and is absent or reduced on migrating primary mesenchyme cells. Microinjection of a monoclonal antibody directed against laminin induces a morphological change in cell shape and a deformation of the embryonic epithelium. Investigation of selected stages of live embryos suggests that the distribution of laminin may be heterogeneous within the basal lamina during early development. The results implicate laminin as a mediator of cell shape change during early morphogenesis.. ...
Institutions: 1) University of Nottingham, 2) University of Vienna. The myodystrophy mouse is a spontaneous, autosomal recessive model of muscular dystrophy. We previously identified the molecular defect underlying the myodystrophy (Largemyd) mouse as a deletion in the Large gene, encoding a glycosyltransferase. This mutant is currently the only animal model for studying the role of glycosylation in the group of human muscle disorders due to mutated glycosyltransferases.Dystroglycan, an extensively modified glycoprotein, is a central component of the dystrophin-associated glycoprotein complex; this plays an important role in maintaining muscle membrane integrity. Furthermore, glycosylation of ?lpha?dystroglycan is necessary for its interaction with the extracellular matrix protein laminin. Western analyses of Largemyd proteins reveal hypo-glycosylation of alpha-dystroglycan and loss of laminin binding. We are currently using the glycan-binding specificities of lectins to investigate the overall ...
in Invasion & Metastasis (1993), 13(1), 1-30. Interactions between cancer cells and laminin, a major component of basement membranes, play a critical role at several steps of the complex process of tumor invasion and metastasis. These interactions ... [more ▼]. Interactions between cancer cells and laminin, a major component of basement membranes, play a critical role at several steps of the complex process of tumor invasion and metastasis. These interactions are mediated through a large variety of cell surface proteins designated as laminin receptors and/or laminin-binding proteins. The growing number of identified laminin binding proteins and domains of this glycoprotein that are biologically active illustrate the complexity of cellular interactions with laminin. The 67-kD laminin receptor (67 LR) was the first protein identified in 1983 as a high-affinity laminin receptor, and its expression is dramatically increased in a large variety of cancer cells. The 67 LR is the subject of several ...
HC11 is a normal mouse mammary epithelial cell line that requires certain growth factors, such as EGF or bFGF, to respond optimally to lactogenic hormones and produce the differentiation marker beta-casein. Growth in insulin (Ins) or PDGF does not produce cells competent to respond to lactogenic hormones. Here we show that competency for differentiation is due at least in part to the modulation of extracellular matrix components. In particular we have studied laminin and tenascin. EGF alters endogenous laminin assembly. In addition, promotion of competency can be partially mimicked by plating HC11 cells on the E8 laminin fragment, which is able to induce lactogenic responsiveness in cells grown in the absence of EGF or bFGF. The production and assembly of tenascin is also dependent upon the growth conditions of the HC11 cells. EGF- or bFGF-grown competent cells produce tenascin but do not assemble it at the extracellular matrix as efficiently as Ins- or PDGF-grown, non-competent cells. This ...
Macrophages require activation with either PMA (Mercurio, A. M., and L. M. Shaw. 1988. J. Cell Biol. 107:1873-1880) or interferon-gamma (Shaw, L. M., and A. M. Mercurio. 1989. J. Exp. Med. 169:303-308) to adhere to a laminin substratum. In the present study, we identified an integrin laminin receptor on macrophages and characterized cellular changes that occur in response to PMA activation that facilitate laminin adhesion. A monoclonal antibody (GoH3) that recognizes the integrin alpha 6 subunit (Sonnenberg, A., H. Janssen, F. Hogervorst, J. Calafat, and J. Hilgers. 1987. J. Biol. Chem. 262:10376-10383) specifically inhibited adhesion to laminin-coated surfaces. This antibody precipitated an alpha 6 beta 1 heterodimer (Mr 130/110 kD) from 125I surface-labeled macrophages. The amount of radiolabeled receptor on the cell surface did not increase after PMA activation. Thus, the induction of laminin adhesion cannot be attributed to de novo or increased surface expression of alpha 6 beta 1. By ...
TY - JOUR. T1 - Changes in the extracellular matrix components fibronectin and laminin during immune rejection of skeletal muscle. AU - Gulati, A. K.. AU - Reddi, A Hari. AU - Zalewski, A. A.. PY - 1984. Y1 - 1984. N2 - Extracellular matrix is known to play an important role during development and maintenance of various tissues. In the present study, changes in two extracellular matrix glycoproteins, fibronec ?ptin and laminin, were investigated in skeletal muscle undergoing immune rejection. Purified antibodies against fibronectin and laminin were used to analyze the matrix by indirect immunofluorescence at various intervals after transplantation of extensor digitorum muscle in rats. Fibronectin and laminin were localized in the pericellular basement membrane zone of the normal myofibers; however, the cytoplasm was devoid of both glycoproteins. Transplanted muscle grafts underwent a process of degeneration and then an initial regeneration during the first 7 days. This regeneration effort ceased ...
Looking for online definition of laminin, alpha 4 in the Medical Dictionary? laminin, alpha 4 explanation free. What is laminin, alpha 4? Meaning of laminin, alpha 4 medical term. What does laminin, alpha 4 mean?
In humans, mutations in the lama2 gene, which encodes laminin a2, cause merosin-deficient congenital muscular dystrophy 1A MDC1A , one subset of a broad
The establishment of epithelial architecture is a complex process involving cross-talk between cells and the basement membrane. Basement membrane assembly requires integrin activity but the role of the associated actomyosin cytoskeleton is poorly understood. Here, we identify the actin-bundling protein T-plastin (Pls3) as a regulator of basement membrane assembly and epidermal morphogenesis. In utero depletion of Pls3 transcripts in mouse embryos caused basement membrane and polarity defects in the epidermis but had little effect on cell adhesion and differentiation. Loss-of-function experiments demonstrated that the apicobasal polarity defects were secondary to the disruption of the basement membrane. However, the basement membrane itself was profoundly sensitive to subtle perturbations in the actin cytoskeleton. We further show that Pls3 localized to the cell cortex, where it was essential for the localization and activation of myosin II. Inhibition of myosin II motor activity disrupted ...
Endothelial basement membranes constitute barriers to extravasating leukocytes during inflammation, a process where laminin isoforms define sites of leukocyte exit; however, how this occurs is poorly understood. In addition to a direct effect on leukocyte transmigration, we show that laminin 511 affects endothelial barrier function by stabilizing VE-cadherin at junctions and downregulating expression of CD99L2, correlating with reduced neutrophil extravasation. Binding of endothelial cells to laminin 511, but not laminin 411 or non-endothelial laminin 111, enhanced transendothelial cell electrical resistance (TEER) and inhibited neutrophil transmigration. Data suggest that endothelial adhesion to laminin 511 via beta1 and beta3 integrins mediates RhoA-induced VE-cadherin localization to cell-cell borders, and while CD99L2 downregulation requires integrin beta1, it is RhoA-independent. Our data demonstrate that molecular information provided by basement membrane laminin 511 affects leukocyte ...
Neuromuscular junction (NMJ) assembly is characterized by the clustering and neuronal alignment of acetylcholine receptors (AChRs). In this study we have addressed post-synaptic contributions to assembly that may arise from the NMJ basement membrane with cultured myotubes. We show that the cell surface-binding LG domains of non-neural (muscle) agrin and perlecan promote AChR clustering in the presence of laminin-2. This type of AChR clustering occurs with a several hour lag, requires muscle-specific kinase (MuSK), and is accompanied by tyrosine phosphorylation of MuSK and betaAChR. It also requires conjugation of the agrin or perlecan to laminin together with laminin polymerization. Furthermore, AChR clustering can be mimicked with antibody binding to non-neural agrin, supporting a mechanism of ligand aggregation. Neural agrin, in addition to its unique ability to cluster AChRs through its B/z sequence insert, also exhibits laminin-dependent AChR clustering, the latter enhancing and stabilizing ...
Objective:To investigate the effects of puerarin on renal structure?function and expressions of MMP-3 and TIMP-1 in the kidney of diabetic rats.Methods:Uninephrectomized STZ-induced male wister rats were used.Puerarin was delivered daily by the intraperitoneal injection from the third day of induction to diabetes for 16 weeks.Using in situ hybridization and immunohistochemistry to detect TIMP-1mRNA expression and MMP-3?TIMP-1?collagen Ⅳ and Laminin expressions in diabetic kidneys with image analysis system;Flow cytometry was used to detect the expressions of MMP-3?TIMP-1.Results:BUN?Ccr and Upro in diabetic rats were significant higher than that in control group.Compared with those in kidneys of control group,diabetic kidneys showed increased expressions of both MMP-3?TIMP-1 proteins and excessive deposition of glomeruli extracellular matrix(P0.05).The blood glucose and the level of expressions of MMP-3?TIMP-1?collagen Ⅳ and Laminin were reduced by puerarin treatment,renal function was improved
Laminin Receptor antibody LS-C197601 is an unconjugated mouse monoclonal antibody to human Laminin Receptor (RPSA). Validated for ELISA and WB.
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In previous studies, researchers at the Cells-in-Motion Cluster of Excellence had discovered that specific proteins in the basement membrane - so-called laminins - influence the migration of the immune cells. For example, they determine the place at which immune cells cross the basement membrane and enter into the tissue. Researchers have now discovered that these components of the basement membrane also affect the inner endothelial cell layer.. A certain laminin - laminin 511 - influences how tightly endothelial cells are connected to each other, explains biochemist Prof. Lydia Sorokin. This is because laminin affects specific molecules that interconnect endothelial cells. For one thing, laminin stabilizes the VE-cadherin molecule at contact sites between adjacent endothelial cells, forming a sort of Velcro fastener between them. In addition, laminin 511 reduces the quantity of a molecule known as CD99L2.. This is found on both the endothelial cells and on the immune cells and permits the ...
Mokkapati, Sharada (2007) Role of the Basement Membrane Proteins Nidogen-1 and -2 and Laminin-Nidogen Interaction in Physiology and Pathology of Skin. [Thesis Abstract]. ...
Geltrex hESC-Qualified, Ready-To-Use, Reduced Growth Factor Basement Membrane Matrix is a soluble form of basement membrane extracted from murine Engelbreth-Holm-Swarm (EHS) tumors. This pre-diluted version of the matrix can be taken directly from the refrigerator and dispensed into cell culture pla
The basement membrane (BM) is a specialized form of extracellular matrix (ECM) underlying epithelia and endothelia and surrounding many types of mesenchymal cells. Nidogen, along with collagen IV and laminin, is a major component of BMs. Although certain ECM proteins such as laminin or reelin influence neuronal function via interactions with cell-surface receptors such as integrins, behavioral neurological impairments due to deficits of BM components have been recognized only recently. Here, alterations in neuronal network function underlying these behavioral changes are revealed. Using nidogen-1 knockout mice, with or without additional heterozygous nidogen-2 knockout (NID1(-/-)/NID2(+/+) or NID1(-/-)/NID2(+/-)), we demonstrate that nidogen is essential for normal neuronal network excitability and plasticity. In nidogen-1 knockouts, seizurelike behavior occurs, and epileptiform spiking was seen in hippocampal in vivo EEG recordings. In vitro, hippocampal field potential recordings revealed that ...
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Stem cell culture is accompanied by extra challenges, and it is critical to maintain genetic stability, ensure stemness, and prevent spontaneous differentiation. Cellartis media and supplements are optimized for derivation, maintenance, and expansion of pluripotent cells.. The Cellartis DEF-CS system is a complete culture system for robust expansion of human iPS cells in a feeder-free and defined environment. A xeno-free version is also available.. iMatrix-511 is a xeno-free recombinant laminin fragment for ES or iPS cell culture substrate; iMatrix-511 provides greater adhesive properties than full-length laminin, vitronectin, or reagents containing an undefined mixture of basement membrane.. ...
Integrin alpha6beta1: A cell surface receptor mediating cell adhesion to the EXTRACELLULAR MATRIX and to other cells via binding to LAMININ. It is involved in cell migration, embryonic development, leukocyte activation and tumor cell invasiveness. Integrin alpha6beta1 is the major laminin receptor on PLATELETS; LEUKOCYTES; and many EPITHELIAL CELLS, and ligand binding may activate a number of signal transduction pathways. Alternative splicing of the cytoplasmic domain of the alpha6 subunit (INTEGRIN ALPHA6) results in the formation of A and B isoforms of the heterodimer, which are expressed in a tissue-specific manner.
Nothing but the greatest affection towards my fellow believers is intended in this post. Theres a popular idea in circulation, commonly known from speaker Louie Giglio, that likes to speak of the Cross-resembling shape of the protein laminin, in conjunction with Colossians 1:17 (He is before all things, and in him all things hold together),…
Nothing but the greatest affection towards my fellow believers is intended in this post. Theres a popular idea in circulation, commonly known from speaker Louie Giglio, that likes to speak of the Cross-resembling shape of the protein laminin, in conjunction with Colossians 1:17 (He is before all things, and in him all things hold together),…
A cell surface receptor mediating cell adhesion to the EXTRACELLULAR MATRIX and to other cells via binding to LAMININ. It is involved in cell migration, embryonic development, leukocyte activation and tumor cell invasiveness. Integrin alpha6beta1 is the major laminin receptor on PLATELETS; LEUKOCYTES; and many EPITHELIAL CELLS, and ligand binding may activate a number of signal transduction pathways. Alternative splicing of the cytoplasmic domain of the alpha6 subunit (INTEGRIN ALPHA6) results in the formation of A and B isoforms of the heterodimer, which are expressed in a tissue-specific manner. . ...
A cell surface receptor mediating cell adhesion to the EXTRACELLULAR MATRIX and to other cells via binding to LAMININ. It is involved in cell migration, embryonic development, leukocyte activation and tumor cell invasiveness. Integrin alpha6beta1 is the major laminin receptor on PLATELETS; LEUKOCYTES; and many EPITHELIAL CELLS, and ligand binding may activate a number of signal transduction pathways. Alternative splicing of the cytoplasmic domain of the alpha6 subunit (INTEGRIN ALPHA6) results in the formation of A and B isoforms of the heterodimer, which are expressed in a tissue-specific manner. . ...
Clone REA276 recognizes the CD239 antigen, a type I membrane glycoprotein also known as basal cell adhesion molecule (BCAM) or Lutheran blood group glycoprotein (LU), which are two alternatively spliced variants of a single immunoglobulin superfamily protein that differ in the length of their cytoplasmic tails. Mouse and human CD239 is highly conserved at the amino acid sequence level. CD239 has a wide tissue distribution and is closely associated with the basal layer of cells in epithelia and the endothelium of blood vessel walls. The expression of CD239 is higher in fetal versus adult tissues, and is upregulated following malignant transformation in some cell types. CD239 is the receptor for laminin alpha-5, an extracellular matrix protein involved in cell differentiation, adhesion, migration, and proliferation. It may plays a role in epithelial cell cancer and in vaso-occlusion of red blood cells in sickle cell disease.Additional information: Clone REA276 displays negligible binding to Fc receptors.
Clone REA276 recognizes the CD239 antigen, a type I membrane glycoprotein also known as basal cell adhesion molecule (BCAM) or Lutheran blood group glycoprotein (LU), which are two alternatively spliced variants of a single immunoglobulin superfamily protein that differ in the length of their cytoplasmic tails. Mouse and human CD239 is highly conserved at the amino acid sequence level. CD239 has a wide tissue distribution and is closely associated with the basal layer of cells in epithelia and the endothelium of blood vessel walls. The expression of CD239 is higher in fetal versus adult tissues, and is upregulated following malignant transformation in some cell types. CD239 is the receptor for laminin alpha-5, an extracellular matrix protein involved in cell differentiation, adhesion, migration, and proliferation. It may plays a role in epithelial cell cancer and in vaso-occlusion of red blood cells in sickle cell disease. Additional information: Clone REA276 displays negligible binding to Fc receptors.
Background and Objective: It is obviously approved that Fibronectin and Laminin α-5 (LAMA5) expression will change, in some organs of fetuses that their mothers were smoking during pregnancy period. For example, the expression of mentioned proteins in newborns decreases in parenchyma of their lungs. In present study, we evaluated the effects ...
Brain-specific homing and direct interactions with the neural substance are prominent hypotheses for brain metastasis formation and a modern manifestation of Pagets seed and soil concept. However, there is little direct evidence for this neurotropic growth in vivo. In contrast, many experimental studies have anecdotally noted the propensity of metastatic cells to grow along the exterior of pre-existing vessels of the CNS, a process termed vascular cooption. These observations suggest the soil for malignant cells in the CNS may well be vascular, rather than neuronal. We used in vivo experimental models of brain metastasis and analysis of human clinical specimens to test this hypothesis. Indeed, over 95% of early micrometastases examined demonstrated vascular cooption with little evidence for isolated neurotropic growth. This vessel interaction was adhesive in nature implicating the vascular basement membrane (VBM) as the active substrate for tumor cell growth in the brain. Accordingly, VBM
Corning™ Matrigel™ Membrane Matrix LDEV-Free; 5mL Corning™ Matrigel™ Membrane Matrix Dissociation Reagents
Science Meets God Many of you are aware of my background in Exercise and Nutritional Sciences, here is where Science meets God. Laminins are major proteins in the basal lamina (one of the layers of the basement membrane), a protein network foundation for most cells and organs. The laminins are an important and biologically active…
Business Owner Linda Morris published a press release titled: The CROSS of Laminin ... The Protein Molecule of the body..linked to the BIBLE ..
Business Owner Linda Morris published a press release titled: Does Laminin hold our body together ... and does Avian Egg Powdered Extract support it ?
Read independent reviews on BD BioCoat Poly-D-Lysine/Laminin 96-well Microplates from BD Biosciences Discovery Labware on SelectScience
Laminin[edit]. Laminins are proteins found in the basal laminae of virtually all animals. Rather than forming collagen-like ... Laminins bind other ECM components such as collagens and nidogens.[9] Development[edit]. There are many cell types that ... Integrins are cell-surface proteins that bind cells to ECM structures, such as fibronectin and laminin, and also to integrin ... fibers, laminins form networks of web-like structures that resist tensile forces in the basal lamina. They also assist in cell ...
Vertebrate laminins[edit]. Netrins share the same terminal structure with vertebrate laminins but appear minimally related. The ... and laminin-10 (α5β1γ1), like the two Caenorhabditis elegans laminins, are embryonically expressed and are essential for ... During the basement assembly process laminins anchor to the cell surface through their G domains after polymerizing through ... Yurchenco PD, Wadsworth WG (October 2004). "Assembly and tissue functions of early embryonic laminins and netrins". Current ...
Laminin, Tissue Inhibitor of Metalloproteinase, or Prolyl Hydroxylase?". Alcoholism: Clinical and Experimental Research. 20 (9 ...
... is made by linking together many small soluble precursor tropoelastin protein molecules (50-70 kDa), to make the final massive insoluble, durable complex. The unlinked tropoelastin molecules are not normally available in the cell, since they become crosslinked into elastin fibres immediately after their synthesis by the cell and during their export into the extracellular matrix. Each tropoelastin consists of a string of 36 small domains, each weighing about 2 kDa in a random coil conformation. The protein consists of alternating hydrophobic and hydrophilic domains, which are encoded by separate exons, so that the domain structure of tropoelastin reflects the exon organization of the gene. The hydrophilic domains contain Lys-Ala (KA) and Lys-Pro (KP) motifs that are involved in crosslinking during the formation of mature elastin. In the KA domains, lysine residues occur as pairs or triplets separated by two or three alanine residues (e.g. AAAKAAKAA) whereas in KP domains the lysine ...
A scleroprotein forms long protein filaments, which are shaped like rods or wires. Scleroproteins are structural proteins or storage proteins that are typically inert and water-insoluble. A scleroprotein occurs as an aggregate due to hydrophobic side chains that protrude from the molecule. A scleroprotein's peptide sequence often has limited residues with repeats; these can form unusual secondary structures, such as a collagen helix. The structures often feature cross-links between chains (e.g., cys-cys disulfide bonds between keratin chains). Scleroproteins tend not to denature as easily as globular proteins. Miroshnikov et al. (1998) are among the researchers who have attempted to synthesize fibrous proteins.[3] ...
... (EC 3.4.24.14, procollagen N-terminal peptidase, procollagen aminopeptidase, aminoprocollagen peptidase, aminoterminal procollagen peptidase, procollagen aminoterminal protease, procollagen N-terminal proteinase, type I/II procollagen N-proteinase, type III procollagen) is an endopeptidase involved in the processing of collagen. The proteases removes the terminal peptides of the procollagen. Deficiency of these enzymes leads to dermatosparaxis or Ehlers-Danlos syndrome.[1] The enzyme is present in the skin of rats and humans.[2] ...
The cytokeratins are encoded by a family encompassing 30 genes. Among them, 20 are epithelial genes and the remaining 10 are specific for trichocytes. All cytokeratin chains are composed of a central α-helix-rich domain (with a 50-90% sequence identity among cytokeratins of the same type and around 30% between cytokeratins of different type) with non-α-helical N- and C-terminal domains. The α-helical domain has 310-150 amino acids and comprises four segments in which a seven-residue pattern repeats. Into this repeated pattern, the first and fourth residues are hydrophobic and the charged residues show alternate positive and negative polarity, resulting in the polar residues being located on one side of the helix. This central domain of the chain provides the molecular alignment in the keratin structure and makes the chains form coiled dimers in solution. The end-domain sequences of type I and II cytokeratin chains contain in both sides of the rod domain the subdomains V1 and V2, which have ...
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LamininEdit. Laminins are proteins found in the basal laminae of virtually all animals. Rather than forming collagen-like ... Integrins are cell-surface proteins that bind cells to ECM structures, such as fibronectin and laminin, and also to integrin ... fibers, laminins form networks of web-like structures that resist tensile forces in the basal lamina. They also assist in cell ...
These markers include CD34 and Laminin. This experiment proves that Prox1 is necessary for lymphatic cells to specify. It is ...
July 1990). "Laminin alterations after in vitro nonenzymatic glycosylation". Diabetes. 39 (7): 807-14. doi:10.2337/diabetes. ...
The basement membrane contains numerous thin collagen fibrils and specialized proteins such as laminin that provide a scaffold ... September 1990). "Distribution and isolation of four laminin variants; tissue restricted distribution of heterotrimers ...
Structurally, netrin resembles the extracellular matrix protein laminin. Netrins are chemotropic; a growing axon will either ... which has several domains which are homologous with laminin at the amino terminal end. The C-terminal domain is where most of ...
Mayer U, Kohfeldt E, Timpl R (October 1998). "Structural and genetic analysis of laminin-nidogen interaction". Annals of the ... retaining their ability to bind laminin. In nematodes, nidogen-1 is necessary for axon guidance, but not for basement membrane ... "Analysis of nidogen-1/laminin γ1 interaction by cross-linking, mass spectrometry, and computational modeling reveals multiple ...
Cerebroglycan has especially high affinity for laminin-1. GPC2 has been suggested as a therapeutic target in neuroblastoma. ...
Growth factors are not expressed or re-expressed; for instance, the extracellular matrix is lacking laminins. Glial scars ... ISBN 978-0-8385-7701-1. Stabenfeldt SE, García AJ, LaPlaca MC (June 2006). "Thermoreversible laminin-functionalized hydrogel ...
Integrin α6β4 operates as a laminin-332 receptor. Integrin α6β4 is composed to two α and β subunit dimers. The larger β4 ... The α6 subunit binds to extracellular BP180, CD151 and laminin-322. When integrin α6β4 binds to Plectin 1a and BPAG1, it ... This is caused by mutations in integrin α6β4, laminin 322 and BPAG2. In dystrophic epidermolysis bullosa, the layers of the ... integrin β4 subunit in the cytoplasm and integrin α6 and laminin-332 in the extracellular space. CD151, a protein of the ...
For patterns containing 0.5 mg/ml laminin, a higher proportion of neurites grew on the microdispensed lines than between the ... Also, the HA hydrogels modified with laminin were able to promote neurite extension. These results support HA gels as a ... The ECM components investigated were laminin-1 and fibronectin, which are natural ECM components, and ProNectin F plus (Pro-F) ... HA hydrogels that were either unmodified or modified with laminin were implanted into an adult central nervous system lesion ...
The majority of CMD cases result from a genetic mutation in laminin-α2, a subunit of the laminin-211 protein, which serves as ... The mouse model of laminin-α2-deficient congenital muscular dystrophy (MDC1A) was found to positively respond to omigapil with ... Colognato H, Yurchenco PD (June 2000). "Form and function: the laminin family of heterotrimers". Dev. Dyn. 218 (2): 213-34. doi ... laminin alpha 2) deficiency" (PDF). EMA/COMP/204694/2008 Rev.1. Muscular Dystrophy Campaign. "New collaboration to support ...
Hinek A (1997). "Biological roles of the non-integrin elastin/laminin receptor". Biological Chemistry. 377 (7-8): 471-80. doi: ... "The 67-kDa enzymatically inactive alternatively spliced variant of beta-galactosidase is identical to the elastin/laminin- ...
Receptor tyrosine kinase activation by laminin G-like domains". J. Biol. Chem. 277 (46): 44164-70. doi:10.1074/jbc.M207340200. ...
Degraded basement membrane fragments (laminin-5) roles to lead the way of mammary epithelial cells migration. Whereas, laminin- ... Laminin and collagen in myoepithelial basement membrane interacting with beta-1 integrin on epithelial surface again, is ... Streuli, C. H.; Schmidhauser, C.; Bailey, N.; Yurchenco, P.; Skubitz, A. P.; Roskelley, C.; Bissell, M. J. (1995). "Laminin ... Dogic, D.; Rousselle, P.; Aumailley, M. (1998). "Cell adhesion to laminin 1 or 5 induces isoform-specific clustering of ...
Among the ligands of integrins are fibronectin, vitronectin, collagen, and laminin. The connection between the cell and the ECM ... Ligands for integrins include fibronectin, vitronectin, collagen and laminin. Integrins are obligate heterodimers composed of α ...
Cell adhesion molecules Fibronectin Laminin Schwab, Manfred, ed. (2001). "Cell Adhesion Molecules". Encyclopedic reference of ...
It contains fibronectin, type IV collagen, perlecan, and laminin. Intraglomerular mesangial cells phagocytize glomerular basal ...
Lander, A. D.; Fujii, D. K.; Reichardt, L. F. (1985). "Laminin is associated with the "neurite outgrowth-promoting factors" ... Calof, A. L.; Lander, A. D. (1991). "Relationship between neuronal migration and cell-substratum adhesion: Laminin and merosin ... of Laminin. Lander serves on the editorial boards of PLOS Biology and the Journal of Biology, is a member of the American ... Isolation of laminin and associated molecules". The Journal of Cell Biology. 101 (3): 898-913. doi:10.1083/jcb.101.3.898. PMC ...
"A Newly Identified Leptospiral Adhesin Mediates Attachment to Laminin". Infect. Immun. 74 (11): 6356-64. doi:10.1128/IAI.00460- ...
Substrates include Col IV, laminin, fibronectin, elastin MMP11. Stromelysin 3. SL-3, ST3, STMY3. secreted. MMP-11 shows more ... type-I transmembrane MMP; substrates include gelatin, fibronectin, laminin MMP16. MT3-MMP. C8orf57, MMP-X2, MT-MMP2, MT-MMP3, ... type-I transmembrane MMP; substrates include gelatin, fibronectin, laminin MMP17. MT4-MMP. MT4-MMP, MMP-17, MT4MMP, MTMMP4. ... type-I transmembrane MMP; substrates include gelatin, fibronectin, laminin MMP15. MT2-MMP. MT2-MMP, MTMMP2, SMCP-2, MMP-15, ...
Examples of these molecules include laminin, fibronectin, tenascin, and perlecan. Some of these are surface bound to cells and ...
Javaherian, K.; Park, S.Y.; Pickl, W.F.; LaMontagne, K.R.; Sjin, R.T.T.; Gillies, S.; Lo, K. (2002). "Laminin modulates ... Oligomeric endostatin (trimer or dimer) binds mainly with laminin of the basal lamina. In-vitro studies have shown endostatin ...
Laminins are composed of 3 non identical chains: laminin alpha, beta and gamma (formerly A, B1, and B2, respectively) and they ... LAMA4, CMD1JJ, LAMA3, LAMA4*-1, Laminin, alpha 4, laminin subunit alpha 4. ... "The complete cDNA sequence of laminin alpha 4 and its relationship to the other human laminin alpha chains". European Journal ... Laminin subunit alpha-4 is a protein that in humans is encoded by the LAMA4 gene.[5][6] ...
Effects of Corning Laminin Effects of Corning Laminin This image of Corning Laminin/Entactin Complex High Concentration in ... Laminin/Entactin Complex Laminin/Entactin Complex Several studies have demonstrated feeder-free culture of hES cell lines on ... Protocol: Laminin Coating on Transwell® Support Protocol: Laminin Coating on Transwell® Support Refer to this simple protocol ... Ultrapure Laminin has the same functionality as standard Laminin but is suited for applications where entactin is not desired. ...
LAMC3 Laminins were previously numbered as they were discovered, i.e. laminin-1, laminin-2, laminin-3, etc., but the ... laminin-211, etc.). In addition, many laminins had common names before either laminin nomenclature was in place. Laminins form ... a laminin-like protein from Caenorhabditis elegans and laminin. Laminin IV domain is not found in short laminin chains (alpha4 ... The laminin N-terminal domain is found in all laminin and netrin subunits except laminin alpha 3A, alpha 4 and gamma 2. LAMA1; ...
immunogen = synthetic peptide: N N Q C H S S H K R R T K, representing the internal region of the human protein according to NP_937762.1. ...
The Laminin database, is a database of non-collagenous extracellular matrix proteins. Laminin Golbert, Daiane C F; Linhares- ... "Laminin database: a tool to retrieve high-throughput and curated data for studies on laminins". Nucleic Acids Res. England. 39 ... "Laminin-database v.2.0: an update on laminins in health and neuromuscular disorders". Nucleic Acids Research. 42 (D1): D426- ...
Crystal structure of three consecutive laminin-type epidermal growth factor-like (LE) modules of laminin gamma1 chain harboring ... Crystal structure of three consecutive laminin-type epidermal growth factor-like (LE) modules of laminin gamma1 chain harboring ... laminin-type EGF-like domain. The number of copies of the LE domain in the different forms of laminins is highly variable; ... In mouse laminin gamma-1 chain, the seventh LE domain has been shown to be the only one that binds with a high affinity to ...
Buy Laminin art prints by Sandra Jean at Imagekind.com. Shop Thousands of Canvas and Framed Wall Art Prints and Posters at ... Tags Jean, Sandra, creation, cross, divine glue, god, laminin, light, love, microscopic, science. ... The reason being is that the photograph of an actual laminin molecule is simply a scattering of black dots with a cluster of ... Similarly, there is a molecule called Laminin within the extracellular matrix which forms the components of our bodies ...
Previously, laminin trimers were numbered with Arabic numerals in the order discovered, that is laminins-1 to -5. We introduce ... Laminins are multidomain heterotrimers composed of alpha, beta and gamma chains. ... A simplification of the laminin nomenclature is presented. ... Laminins are multidomain heterotrimers composed of alpha, beta ... For example, the laminin with the chain composition alpha5beta1gamma1 is termed laminin-511, and not laminin-10. The current ...
laminin subunit gamma-3. Names. laminin, gamma 3. laminin-12 subunit gamma. laminin-14 subunit gamma. laminin-15 subunit gamma ... Laminin_B; Laminin B (Domain IV). pfam00053. Location:917 → 965. Laminin_EGF; Laminin EGF domain. pfam00055. Location:44 → 269 ... Laminin_B; Laminin B (Domain IV). pfam00053. Location:917 → 965. Laminin_EGF; Laminin EGF domain. pfam00055. Location:44 → 269 ... Laminin_B; Laminin B (Domain IV). pfam00053. Location:917 → 965. Laminin_EGF; Laminin EGF domain. pfam00055. Location:44 → 269 ...
If you know of any papers that use this antibody, please contact us at antibodies [at] alzforum [dot] org for consideration in the References section.. ...
Laminin 332 (LN332) is a basally expressed extracellular matrix protein that enhances the migration and invasion of breast ... Laminin 332 expression in breast carcinoma Appl Immunohistochem Mol Morphol. 2012 Mar;20(2):159-64. doi: 10.1097/PAI. ... Laminin 332 (LN332) is a basally expressed extracellular matrix protein that enhances the migration and invasion of breast ... Keywords: Basal-like phenotype; Breast carcinoma; HER2 dual in situ hybridization; Immunohistochemistry; Laminin 332; Triple ...
Therefore, the association of T. vaginalis with immobilized fibronectin (FN) and laminin (LM) on cover-slips was examined. ... Trichomonas vaginalis interactions with fibronectin and laminin.. Crouch ML1, Alderete JF. ...
Rabbit polyclonal Laminin antibody validated for WB, IHC, ICC/IF and tested in Mouse and Rat. Referenced in 14 publications. ... Contains 11 laminin EGF-like domains.. Contains 1 laminin IV type A domain.. Contains 1 laminin N-terminal domain. ... The alpha-helical domains I and II are thought to interact with other laminin chains to form a coiled coil structure.. Domain ... Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of ...
LNSC produced Laminin α5 (Lama5) regulates CD4 T cells but the underlying mechanisms of its functions are poorly understood. ...
Laminin Alterations After In Vitro Nonenzymatic Glycosylation. Aristidis S Charonis, Lorrel A Reger, Jay E Dege, Kokkona Kouzi- ... Laminin Alterations After In Vitro Nonenzymatic Glycosylation. Aristidis S Charonis, Lorrel A Reger, Jay E Dege, Kokkona Kouzi- ... Laminin Alterations After In Vitro Nonenzymatic Glycosylation Message Subject (Your Name) has forwarded a page to you from ...
Rabbit polyclonal Laminin 5 antibody validated for WB, IP, ELISA, IHC, ICC/IF and tested in Human and Mouse. Referenced in 28 ... Laminins, composed of 3 non identical chains: laminin alpha, beta and gamma (formerly A, B1, and B2, respectively), have a ... Each laminin chain is a multidomain protein encoded by a distinct gene. Several isoforms of each chain have been described. ... Anti-Laminin 5 antibody (ab14509) at 5 µg/ml + Cell culture supernatant of keratinocytes at 12 µl. Secondary. Peroxidase ...
Iodinated P1 fragment of laminin, which retains the laminin-binding site, binds saturably to the parasite surface with a Kd of ... Presence of laminin-binding proteins in trichomonads and their role in adhesion. F Casta e Silva Filho, W de Souza, and J D ... We report that laminin enhances the adhesion of the parasitic protozoa Trichomonas vaginalis and Tritrichomonas foetus to a ... Addition of anti-laminin antibodies to medium significantly inhibited the adhesion of parasites to polystyrene substrate. ...
This review deals with biological and pathological aspects of various isoforms of the matrix molecules fibronectin and laminin ... Iivanainen A, Vuolteenaho R, Saino K, Eddy R, Shows TB, Sariola H, Tryggvason K (1994) The human laminin β2 chain (s-laminin): ... Martin PT, Ettinger AJ, Sanes JR (1995) A synaptic localization domain in the synaptic cleft protein laminin β2 (s-laminin). ... The current nomenclature of the laminin molecule family is presented. The laminin chain constitution of basement membranes ...
Compare laminin, alpha 3 ELISA Kits from leading suppliers on Biocompare. View specifications, prices, citations, reviews, and ... Your search returned 115 laminin, alpha 3 ELISA ELISA Kit across 13 suppliers. ... Rat Laminin PicoKine ELISA Kit *Detection Target: Laminin subunit alpha-1; Laminin subunit alpha-2; Laminin ... Human Laminin PicoKine ELISA Kit *Detection Target: Laminin subunit alpha-1; Laminin subunit alpha-2; Laminin ...
Mutations in the laminin β subunit (encoded by Laminin B1, LanB1) or β subunit (encoded by laminin B2, lanB2) have not been ... The experiments showing that in laminin β mutant embryos the laminin α3,5 and laminin α1,2 chains are not present at BMs or ... Laminin B1: Biological Overview , References Gene name - Laminin B1 Synonyms - Cytological map position - 28C4-28D1 Function - ... In absence of laminin β, other laminin subunits as well as major BMs components fail to assemble into BMs. This analysis ...
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This antibody is designed to detect laminin using western blotting analysis under non-reducing and non-heating conditions and ... Laminin gamma 1 chain (N‑terminus). M020. IHC. WB. Human. (LN82-13). Mouse IgG MoAb. Cross-reacts with rabbit laminin. Does not ... ELISAs for Laminin Detection. The Laminin EIA Kit is a solid-phase sandwich ELISA that utilizes two specific mouse monoclonal ... Laminin Detection. Laminin (LN) proteins are part of a large family of extracellular matrix basal laminal glycoproteins and ...
Mouse Laminin I may be used as a gel or as a media additive to study cellular growth and differentiation in three dimensions in ... Thaw 3-D Culture Matrix Laminin I at 4 ⁰C overnight.. *Working on ice, add 250 μl of 3-D Culture Matrix Laminin I to each well ... Any unused Laminin I can be stored at 4 ⁰C up to one week or stored in working aliquots at -20 ⁰C in a manual defrost freezer. ... 3D culture - Laminin I promotes attachment and growth of a human epithelial cell line derived from mammary gland (MCF-10A) and ...
Daily News How Gaining and Losing Weight Affects the Body Millions of measurements from 23 people who consumed extra calories every day for a month reveal changes in proteins, metabolites, and gut microbiota that accompany shifts in body mass.. ...
1997). However, the major constituent of the basal lamina of adult skeletal muscle is not laminin 111, but laminin 211 (Sanes ... A major constituent of the basal lamina of adult skeletal muscle is Laminin 211 (also known as merosin). Laminin is a ... Laminin 211 plays a key role in promoting muscle cell adhesion to the extracellular matrix and thus in cell survival. It binds ... Through binding to laminin 211, it thus connects the extracellular matrix through to cytoskeletal γ actin on the inside of the ...
Anyone out there that would recommend a good Pan-Laminin antibody suitable for IHC-P and reactivity with mouse tissue? Many ... Pan-Laminin - posted in General Lab Techniques: Hi ... Pan-Laminin. Started by BioS, Jun 14 2018 08:37 AM laminin IHC ... Also tagged with one or more of these keywords: laminin, IHC, antibody. Protocols and Techniques Forums → General Lab ... Anyone out there that would recommend a good Pan-Laminin antibody suitable for IHC-P and reactivity with mouse tissue? ...
Rabbit Polyclonal Anti-Laminin Antibody Basement Membrane Marker cited in 65 publications. Validated: WB, Flow, ICC/IF, IHC, ... Laminin Antibody Summary. Immunogen. Laminin Antibody was made to Laminin 111 isolated from mouse Engelbreth-Holm-Swarm (EHS) ... Laminin Antibody is pan-specific and reacts well with all Laminin isoforms tested: Laminin-1 (alpha-1, beta-1, and gamma-1) and ... Laminin Antibody [NB300-144] is pan-specific and reacts well with all Laminin isoforms tested: Laminin-1 (alpha-1, beta-1, and ...
... it states that with cancer metastasis laminin and integrin receptors are increased. Can someone explain what role that has in ... In FA 2009 on pg 214, it states that with cancer metastasis laminin and integrin receptors are increased. Can someone explain ... In FA 2009 on pg 214, it states that with cancer metastasis laminin and integrin receptors are increased. Can someone explain ... laminins and integrins are proteins that allow the cancer cells to attach to the basement membrane (in the same way that ...
... laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by ... IPR009254, Laminin_aI. IPR010307, Laminin_dom_II. IPR002049, Laminin_EGF. IPR001791, Laminin_G. IPR000034, Laminin_IV. ... IPR009254, Laminin_aI. IPR010307, Laminin_dom_II. IPR002049, Laminin_EGF. IPR001791, Laminin_G. IPR000034, Laminin_IV. ... PF00052, Laminin_B, 1 hit. PF00053, Laminin_EGF, 6 hits. PF00054, Laminin_G_1, 1 hit. PF02210, Laminin_G_2, 4 hits. ...
Ausgesuchte Qualitäts-Hersteller für Laminin Antikörper. Hier bestellen. ... Monoklonale und polyklonale Laminin Antikörper für viele Methoden. ... laminin subunit alpha-2-like , laminin M chain , laminin subunit alpha-2 , laminin-12 subunit alpha , laminin-2 subunit alpha ... laminin alpha 2 subunit , laminin, alpha 2 (merosin, congenital muscular dystrophy) , laminin, alpha 2 , caf , candyfloss , ...

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