AnatomyOrganismsChemicals and DrugsAnalytical, Diagnostic and Therapeutic Techniques and EquipmentPhenomena and ProcessesDisciplines and OccupationsTechnology, Industry, AgricultureInformation ScienceHealth Care
Lactococcus lactisLactococcusNisinCheeseBacterial ProteinsMolecular Sequence DataBacteriophagesBacteriocinsGenes, BacterialSiphoviridaeGene Expression Regulation, BacterialLeuconostocLactobacillusBase SequenceDNA, BacterialFermentationPlasmidsAmino Acid SequenceKluyveromycesCloning, MolecularMilkOperonSequence Analysis, DNALactoseCultured Milk ProductsAcetoinConjugation, GeneticDiacetylOpen Reading FramesStreptococcus thermophilusFood MicrobiologyFood IndustryStreptococcus PhagesGenetic EngineeringCulture MediaPediococcusChromosomes, BacterialDairy ProductsPhosphofructokinasesRestriction MappingEscherichia coliSequence Homology, Amino AcidIndustrial MicrobiologyCholatesBacteriolysisAminopeptidasesLactobacillus plantarumCaseinsDNA Transposable ElementsTransformation, BacterialLactic AcidCell WallFood TechnologyStreptococcusPhosphoglucomutaseBifidobacteriumMicrococcal NucleaseL-Lactate DehydrogenaseLactobacillaceae
Lactococcus lactis
A non-pathogenic species of LACTOCOCCUS found in DAIRY PRODUCTS and responsible for the souring of MILK and the production of LACTIC ACID.
Lactococcus
A genus of gram-positive, coccoid bacteria mainly isolated from milk and milk products. These bacteria are also found in plants and nonsterile frozen and dry foods. Previously thought to be a member of the genus STREPTOCOCCUS (group N), it is now recognized as a separate genus.
Nisin
A 34-amino acid polypeptide antibiotic produced by Streptococcus lactis. It has been used as a food preservative in canned fruits and vegetables, and cheese.
Cheese
A nutritious food consisting primarily of the curd or the semisolid substance formed when milk coagulates.
Bacterial Proteins
Proteins found in any species of bacterium.
Molecular Sequence Data
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
Bacteriophages
Viruses whose hosts are bacterial cells.
Bacteriocins
Substances elaborated by specific strains of bacteria that are lethal against other strains of the same or related species. They are protein or lipopolysaccharide-protein complexes used in taxonomy studies of bacteria.
Genes, Bacterial
The functional hereditary units of BACTERIA.
Siphoviridae
A family of BACTERIOPHAGES and ARCHAEAL VIRUSES which are characterized by long, non-contractile tails.
Gene Expression Regulation, Bacterial
Any of the processes by which cytoplasmic or intercellular factors influence the differential control of gene action in bacteria.
Leuconostoc
A genus of gram-positive, facultatively anaerobic bacteria whose growth is dependent on the presence of a fermentable carbohydrate. It is nonpathogenic to plants and animals, including humans.
Lactobacillus
A genus of gram-positive, microaerophilic, rod-shaped bacteria occurring widely in nature. Its species are also part of the many normal flora of the mouth, intestinal tract, and vagina of many mammals, including humans. Pathogenicity from this genus is rare.
Base Sequence
The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.
DNA, Bacterial
Deoxyribonucleic acid that makes up the genetic material of bacteria.
Fermentation
Anaerobic degradation of GLUCOSE or other organic nutrients to gain energy in the form of ATP. End products vary depending on organisms, substrates, and enzymatic pathways. Common fermentation products include ETHANOL and LACTIC ACID.
Plasmids
Extrachromosomal, usually CIRCULAR DNA molecules that are self-replicating and transferable from one organism to another. They are found in a variety of bacterial, archaeal, fungal, algal, and plant species. They are used in GENETIC ENGINEERING as CLONING VECTORS.
Amino Acid Sequence
The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.
Kluyveromyces
An ascomycetous yeast of the fungal family Saccharomycetaceae, order SACCHAROMYCETALES.
Cloning, Molecular
The insertion of recombinant DNA molecules from prokaryotic and/or eukaryotic sources into a replicating vehicle, such as a plasmid or virus vector, and the introduction of the resultant hybrid molecules into recipient cells without altering the viability of those cells.
Milk
The white liquid secreted by the mammary glands. It contains proteins, sugar, lipids, vitamins, and minerals.
Operon
In bacteria, a group of metabolically related genes, with a common promoter, whose transcription into a single polycistronic MESSENGER RNA is under the control of an OPERATOR REGION.
Sequence Analysis, DNA
A multistage process that includes cloning, physical mapping, subcloning, determination of the DNA SEQUENCE, and information analysis.
Lactose
A disaccharide of GLUCOSE and GALACTOSE in human and cow milk. It is used in pharmacy for tablets, in medicine as a nutrient, and in industry.
Cultured Milk Products
Milk modified with controlled FERMENTATION. This should not be confused with KAFFIR LIME or with KAFFIR CORN.
Acetoin
A product of fermentation. It is a component of the butanediol cycle in microorganisms. In mammals it is oxidized to carbon dioxide.
Conjugation, Genetic
A parasexual process in BACTERIA; ALGAE; FUNGI; and ciliate EUKARYOTA for achieving exchange of chromosome material during fusion of two cells. In bacteria, this is a uni-directional transfer of genetic material; in protozoa it is a bi-directional exchange. In algae and fungi, it is a form of sexual reproduction, with the union of male and female gametes.
Diacetyl
Carrier of aroma of butter, vinegar, coffee, and other foods.
Open Reading Frames
A sequence of successive nucleotide triplets that are read as CODONS specifying AMINO ACIDS and begin with an INITIATOR CODON and end with a stop codon (CODON, TERMINATOR).
Streptococcus thermophilus
A species of thermophilic, gram-positive bacteria found in MILK and milk products.
Food Microbiology
The presence of bacteria, viruses, and fungi in food and food products. This term is not restricted to pathogenic organisms: the presence of various non-pathogenic bacteria and fungi in cheeses and wines, for example, is included in this concept.
Food Industry
The industry concerned with processing, preparing, preserving, distributing, and serving of foods and beverages.
Streptococcus Phages
Viruses whose host is Streptococcus.
Genetic Engineering
Directed modification of the gene complement of a living organism by such techniques as altering the DNA, substituting genetic material by means of a virus, transplanting whole nuclei, transplanting cell hybrids, etc.
Culture Media
Any liquid or solid preparation made specifically for the growth, storage, or transport of microorganisms or other types of cells. The variety of media that exist allow for the culturing of specific microorganisms and cell types, such as differential media, selective media, test media, and defined media. Solid media consist of liquid media that have been solidified with an agent such as AGAR or GELATIN.
Pediococcus
A genus of gram-positive, facultatively anaerobic bacteria whose growth is dependent on the presence of a fermentable carbohydrate. No endospores are produced. Its organisms are found in fermenting plant products and are nonpathogenic to plants and animals, including humans.
Chromosomes, Bacterial
Structures within the nucleus of bacterial cells consisting of or containing DNA, which carry genetic information essential to the cell.
Dairy Products
Raw and processed or manufactured milk and milk-derived products. These are usually from cows (bovine) but are also from goats, sheep, reindeer, and water buffalo.
Phosphofructokinases
Allosteric enzymes that regulate glycolysis and gluconeogenesis. These enzymes catalyze phosphorylation of fructose-6-phosphate to either fructose-1,6-bisphosphate (PHOSPHOFRUCTOKINASE-1 reaction), or to fructose-2,6-bisphosphate (PHOSPHOFRUCTOKINASE-2 reaction).
Restriction Mapping
Use of restriction endonucleases to analyze and generate a physical map of genomes, genes, or other segments of DNA.
Escherichia coli
A species of gram-negative, facultatively anaerobic, rod-shaped bacteria (GRAM-NEGATIVE FACULTATIVELY ANAEROBIC RODS) commonly found in the lower part of the intestine of warm-blooded animals. It is usually nonpathogenic, but some strains are known to produce DIARRHEA and pyogenic infections. Pathogenic strains (virotypes) are classified by their specific pathogenic mechanisms such as toxins (ENTEROTOXIGENIC ESCHERICHIA COLI), etc.
Sequence Homology, Amino Acid
The degree of similarity between sequences of amino acids. This information is useful for the analyzing genetic relatedness of proteins and species.
Industrial Microbiology
The study, utilization, and manipulation of those microorganisms capable of economically producing desirable substances or changes in substances, and the control of undesirable microorganisms.
Cholates
Salts and esters of CHOLIC ACID.
Bacteriolysis
Rupture of bacterial cells due to mechanical force, chemical action, or the lytic growth of BACTERIOPHAGES.
Aminopeptidases
A subclass of EXOPEPTIDASES that act on the free N terminus end of a polypeptide liberating a single amino acid residue. EC 3.4.11.
Lactobacillus plantarum
A species of rod-shaped, LACTIC ACID bacteria used in PROBIOTICS and SILAGE production.
Caseins
A mixture of related phosphoproteins occurring in milk and cheese. The group is characterized as one of the most nutritive milk proteins, containing all of the common amino acids and rich in the essential ones.
DNA Transposable Elements
Discrete segments of DNA which can excise and reintegrate to another site in the genome. Most are inactive, i.e., have not been found to exist outside the integrated state. DNA transposable elements include bacterial IS (insertion sequence) elements, Tn elements, the maize controlling elements Ac and Ds, Drosophila P, gypsy, and pogo elements, the human Tigger elements and the Tc and mariner elements which are found throughout the animal kingdom.
Transformation, Bacterial
The heritable modification of the properties of a competent bacterium by naked DNA from another source. The uptake of naked DNA is a naturally occuring phenomenon in some bacteria. It is often used as a GENE TRANSFER TECHNIQUE.
Lactic Acid
A normal intermediate in the fermentation (oxidation, metabolism) of sugar. The concentrated form is used internally to prevent gastrointestinal fermentation. (From Stedman, 26th ed)
Cell Wall
The outermost layer of a cell in most PLANTS; BACTERIA; FUNGI; and ALGAE. The cell wall is usually a rigid structure that lies external to the CELL MEMBRANE, and provides a protective barrier against physical or chemical agents.
Food Technology
The application of knowledge to the food industry.
Streptococcus
A genus of gram-positive, coccoid bacteria whose organisms occur in pairs or chains. No endospores are produced. Many species exist as commensals or parasites on man or animals with some being highly pathogenic. A few species are saprophytes and occur in the natural environment.
Phosphoglucomutase
An enzyme that catalyzes the conversion of alpha D-glucose 1-phosphate to alpha D-glucose 6-phosphate. EC 5.4.2.2.
Bifidobacterium
A rod-shaped, gram-positive, non-acid-fast, non-spore-forming, non-motile bacterium that is a genus of the family Bifidobacteriaceae, order Bifidobacteriales, class ACTINOBACTERIA. It inhabits the intestines and feces of humans as well as the human vagina.
Micrococcal Nuclease
An enzyme that catalyzes the endonucleolytic cleavage to 3'-phosphomononucleotide and 3'-phospholigonucleotide end-products. It can cause hydrolysis of double- or single-stranded DNA or RNA. (From Enzyme Nomenclature, 1992) EC 3.1.31.1.
L-Lactate Dehydrogenase
A tetrameric enzyme that, along with the coenzyme NAD+, catalyzes the interconversion of LACTATE and PYRUVATE. In vertebrates, genes for three different subunits (LDH-A, LDH-B and LDH-C) exist.
Lactobacillaceae
A family of gram-positive bacteria found regularly in the mouth and intestinal tract of man and other animals, in food and dairy products, and in fermenting vegetable juices. A few species are highly pathogenic.

Novel characteristic for distinguishing Lactococcus lactis subsp. lactis from subsp. cremoris. (1/1459)

Lactococcus lactis strains were examined for their ability to produce gamma-aminobutyric acid (GABA). Results showed that strains of L. lactis subsp. lactis were able to produce this acid, whereas L. lactis subsp. cremoris were not. GABA production thus represents another effective characteristic for distinguishing L. lactis subsp. lactis from L. lactis subsp. cremoris.  (+info)

Mechanism of citrate metabolism in Lactococcus lactis: resistance against lactate toxicity at low pH. (2/1459)

Measurement of the flux through the citrate fermentation pathway in resting cells of Lactococcus lactis CRL264 grown in a pH-controlled fermentor at different pH values showed that the pathway was constitutively expressed, but its activity was significantly enhanced at low pH. The flux through the citrate-degrading pathway correlated with the magnitude of the membrane potential and pH gradient that were generated when citrate was added to the cells. The citrate degradation rate and proton motive force were significantly higher when glucose was metabolized at the same time, a phenomenon that could be mimicked by the addition of lactate, the end product of glucose metabolism. The results clearly demonstrate that citrate metabolism in L. lactis is a secondary proton motive force-generating pathway. Although the proton motive force generated by citrate in cells grown at low pH was of the same magnitude as that generated by glucose fermentation, citrate metabolism did not affect the growth rate of L. lactis in rich media. However, inhibition of growth by lactate was relieved when citrate also was present in the growth medium. Citrate did not relieve the inhibition by other weak acids, suggesting a specific role of the citrate transporter CitP in the relief of inhibition. The mechanism of citrate metabolism presented here provides an explanation for the resistance to lactate toxicity. It is suggested that the citrate metabolic pathway is induced under the acidic conditions of the late exponential growth phase to make the cells (more) resistant to the inhibitory effects of the fermentation product, lactate, that accumulates under these conditions.  (+info)

A general method for selection of alpha-acetolactate decarboxylase-deficient Lactococcus lactis mutants to improve diacetyl formation. (3/1459)

The enzyme acetolactate decarboxylase (Ald) plays a key role in the regulation of the alpha-acetolactate pool in both pyruvate catabolism and the biosynthesis of the branched-chain amino acids, isoleucine, leucine, and valine (ILV). This dual role of Ald, due to allosteric activation by leucine, was used as a strategy for the isolation of Ald-deficient mutants of Lactococcus lactis subsp. lactis biovar diacetylactis. Such mutants can be selected as leucine-resistant mutants in ILV- or IV-prototrophic strains. Most dairy lactococcus strains are auxotrophic for the three amino acids. Therefore, the plasmid pMC004 containing the ilv genes (encoding the enzymes involved in the biosynthesis of IV) of L. lactis NCDO2118 was constructed. Introduction of pMC004 into ILV-auxotrophic dairy strains resulted in an isoleucine-prototrophic phenotype. By plating the strains on a chemically defined medium supplemented with leucine but not valine and isoleucine, spontaneous leucine-resistant mutants were obtained. These mutants were screened by Western blotting with Ald-specific antibodies for the presence of Ald. Selected mutants lacking Ald were subsequently cured of pMC004. Except for a defect in the expression of Ald, the resulting strain, MC010, was identical to the wild-type strain, as shown by Southern blotting and DNA fingerprinting. The mutation resulting in the lack of Ald in MC010 occurred spontaneously, and the strain does not contain foreign DNA; thus, it can be regarded as food grade. Nevertheless, its application in dairy products depends on the regulation of genetically modified organisms. These results establish a strategy to select spontaneous Ald-deficient mutants from transformable L. lactis strains.  (+info)

Characterization of the divergent sacBK and sacAR operons, involved in sucrose utilization by Lactococcus lactis. (4/1459)

The divergently transcribed sacBK and sacAR operons, which are involved in the utilization of sucrose by Lactococcus lactis NZ9800, were examined by transcriptional and gene inactivation studies. Northern analyses of RNA isolated from cells grown at the expense of different carbon sources revealed three sucrose-inducible transcripts: one of 3.2 kb containing sacB and sacK, a second of 3.4 kb containing sacA and sacR, and a third of 1.8 kb containing only sacR. The inactivation of the sacR gene by replacement recombination resulted in the constitutive transcription of the sacBK and sacAR operons in the presence of different carbon sources, indicating that SacR acts as a repressor of transcription.  (+info)

Membrane topology of the lactococcal bacteriocin ATP-binding cassette transporter protein LcnC. Involvement of LcnC in lactococcin a maturation. (5/1459)

Many non-lantibiotic bacteriocins of lactic acid bacteria are produced as precursors with N-terminal leader peptides different from those present in preproteins exported by the general sec-dependent (type II) secretion pathway. These bacteriocins utilize a dedicated (type I) secretion system for externalization. The secretion apparatus for the lactococcins A, B, and M/N (LcnA, B, and M/N) from Lactococcus lactis is composed of the two membrane proteins LcnC and LcnD. LcnC belongs to the ATP-binding cassette transporters, whereas LcnD is a protein with similarities to other accessory proteins of type I secretion systems. This paper shows that the N-terminal part of LcnC is involved in the processing of the precursor of LcnA. By making translational fusions of LcnC to the reporter proteins beta-galactosidase (LacZ) and alkaline phosphatase (PhoA*), it was shown that both the N- and C-terminal parts of LcnC are located in the cytoplasm. As the N terminus of LcnC is required for LcnA maturation and is localized in the cytoplasm, we conclude that the processing of the bacteriocin LcnA to its mature form takes place at the cytosolic side of the cytoplasmic membrane.  (+info)

Hydrolysis of alphas1- and beta-casein-derived peptides with a broad specificity aminopeptidase and proline specific aminopeptidases from Lactococcus lactis subsp. cremoris AM2. (6/1459)

Aminopeptidase hydrolysis of alpha(s)1 - and beta-casein-derived synthetic peptides containing non-consecutive and consecutive proline residues was characterised. Aminopeptidase P (Pep P) (EC 3.4.11.9) or post-proline dipeptidyl aminopeptidase (PPDA) (EC 3.4.14.5) along with lysine-paranitroanilide hydrolase (KpNA-H) (EC 3.4.11.1) activities are required in the degradation of peptides containing non-consecutive proline residues. However, both Pep P and PPDA along with KpNA-H are required for hydrolysis of peptides containing consecutive proline residues. The results demonstrate the mechanism by which combinations of purified general and proline specific aminopeptidases from Lactococcus lactis subsp. cremoris AM2 hydrolyse peptides containing proline residues.  (+info)

Regulation of expression of the Lactococcus lactis histidine operon. (7/1459)

In Lactococcus lactis, the his operon contains all the genes necessary for histidine biosynthesis. It is transcribed from a unique promoter, localized 300 bp upstream of the first gene. The region corresponding to the untranslated 5' end of the transcript, named the his leader region, displays the typical features of the T box transcriptional attenuation mechanism which is involved in the regulation of many amino acid biosynthetic operons and tRNA synthetase genes in gram-positive bacteria. Here we describe the regulation of transcription of the his operon by the level of histidine in the growth medium. In the absence of histidine, two transcripts are present. One covers the entire operon, while the other stops at a terminator situated about 250 bp downstream of the transcription start point. DNA sequences implicated in regulation of the his operon were identified by transcriptional fusion with luciferase genes and site-directed mutagenesis. In addition to the previously defined sequences necessary for effective T-box-mediated regulation, new essential regions were identified. Eighteen percent of the positions of the his leader region were found to differ in seven distantly related strains of L. lactis. Analysis of the variable positions supports the folding model of the central part of the his leader region. Lastly, in addition to the T-box-mediated regulation, the operon is regulated at the level of initiation of transcription, which is repressed in the presence of histidine. An operator site, necessary for full repression, overlaps the terminator involved in the T box attenuation mechanism. The functionality of the operator is altered on plasmids with low and high copy numbers, suggesting that supercoiling may play a role in the expression of the his operon. The extents of regulation at the levels of initiation and attenuation of transcription are 6- to 8-fold and 14-fold, respectively. Together, the two levels of control allow a 120-fold range of regulation of the L. lactis operon by histidine.  (+info)

Disruption and analysis of the clpB, clpC, and clpE genes in Lactococcus lactis: ClpE, a new Clp family in gram-positive bacteria. (8/1459)

In the genome of the gram-positive bacterium Lactococcus lactis MG1363, we have identified three genes (clpC, clpE, and clpB) which encode Clp proteins containing two conserved ATP binding domains. The proteins encoded by two of the genes belong to the previously described ClpB and ClpC families. The clpE gene, however, encodes a member of a new Clp protein family that is characterized by a short N-terminal domain including a putative zinc binding domain (-CX2CX22CX2C-). Expression of the 83-kDa ClpE protein as well as of the two proteins encoded by clpB was strongly induced by heat shock and, while clpC mRNA synthesis was moderately induced by heat, we were unable to identify the ClpC protein. When we analyzed mutants with disruptions in clpB, clpC, or clpE, we found that although the genes are part of the L. lactis heat shock stimulon, the mutants responded like wild-type cells to heat and salt treatments. However, when exposed to puromycin, a tRNA analogue that results in the synthesis of truncated, randomly folded proteins, clpE mutant cells formed smaller colonies than wild-type cells and clpB and clpC mutant cells. Thus, our data suggest that ClpE, along with ClpP, which recently was shown to participate in the degradation of randomly folded proteins in L. lactis, could be necessary for degrading proteins generated by certain types of stress.  (+info)

Modeling of nisin production by Lactococcus lactis</em>...
TY - CONF. T1 - Modeling of nisin production by Lactococcus lactis. AU - Pongtharangkul, Thunyarat. AU - Demirci, Ali. AU - Puri, Virendra. PY - 2007/11/7. Y1 - 2007/11/7. N2 - Nisin, the only FDA approved bacteriocin, is widely used as a natural food preservative against Gram-positive bacteria. It is produced by lactic acid bacteria Lactococcus lactis subsp. lactis during fermentation. Mathematical models of biomass and nisin production during fermentation not only provide information about the kinetic-metabolic nature of nisin, but also facilitate the control and optimization of nisin production. In this study, several models were developed to describe biomass, nisin, and lactic acid production as well as sucrose consumption during nisin fermentation by L. lactis in suspended-cell batch culture. All models were designed so that they contained biologically relevant parameters. The re-modified logistic and Gompertz models proposed in this study adequately described the growth and the validation ...
https://pennstate.pure.elsevier.com/en/publications/modeling-of-nisin-production-by-lactococcus-lactis
Metabolic characterization and transformation of the non-dairy Lactococcus lactis strain KF147, for production of ethanol from...Metabolic characterization and transformation of the non-dairy Lactococcus lactis strain KF147, for production of ethanol from...
TY - JOUR. T1 - Metabolic characterization and transformation of the non-dairy Lactococcus lactis strain KF147, for production of ethanol from xylose. AU - Petersen, Kia Vest. AU - Liu, Jianming. AU - Chen, Jun. AU - Martinussen, Jan. AU - Jensen, Peter Ruhdal. AU - Solem, Christian. PY - 2017. Y1 - 2017. N2 - The non-dairy lactic acid bacterium Lactococcus lactis KF147 can utilize xylose as the sole energy source. To assess whether KF147 could serve as a platform organism for converting second generation sugars into useful chemicals, we characterized growth and product formation for KF147 when grown on xylose. In a defined medium KF147 was found to co-metabolize xylose and arginine, resulting in bi-phasic growth. Especially at low xylose concentrations, arginine significantly improved growth rate. To facilitate further studies of the xylose metabolism, we eliminated arginine catabolism by deleting the arcA gene encoding the arginine deiminase. The fermentation product profile suggested two ...
https://orbit.dtu.dk/en/publications/metabolic-characterization-and-transformation-of-the-non-dairy-la
Isolation and properties of Lactococcus lactis subsp. lactis biovar diacetylactis CNRZ 483 mutants producing diacetyl and...Isolation and properties of Lactococcus lactis subsp. lactis biovar diacetylactis CNRZ 483 mutants producing diacetyl and...
Following treatment with the mutagen N-methyl-N-nitro-N-nitrosoguanidine, three mutants of Lactococcus lactis subsp. lactis biovar diacetylactis CNRZ 483 that produced diacetyl and acetoin from glucose were isolated. The lactate dehydrogenase activity of these mutants was strongly attenuated, and the mutants produced less lactate than the parental strain. The kinetic properties of lactate dehydrogenase of strain CNRZ 483 and the mutants revealed differences in the affinity of the enzyme for pyruvate, NADH, and fructose-1,6-diphosphate. When cultured aerobically, strain CNRZ 483 transformed 2.3% of glucose to acetoin and produced no diacetyl or 2,3-butanediol. Under the same conditions, mutants 483L1, 483L2, and 483L3 transformed 42.0, 78.9, and 75.8%, respectively, of glucose to C4 compounds (diacetyl, acetoin, and 2,3-butanediol). Anaerobically, strain CNRZ 483 produced no C4 compounds, while mutants 483L1, 483L2, and 483L3 transformed 2.0, 37.0, and 25.8% of glucose to acetoin and 2,3-butanediol. In
https://www.semanticscholar.org/paper/Isolation-and-properties-of-Lactococcus-lactis-sub-Boumerdassi-Monnet/897bdc7ed0ff2d2e37f20f7bb1195ee9c9c89532
Draft Genome Sequences of Three Amino Acid-Secreting Lactococcus lactis Strains - Research database - University of GroningenDraft Genome Sequences of Three Amino Acid-Secreting Lactococcus lactis Strains - Research database - University of Groningen
TY - JOUR. T1 - Draft Genome Sequences of Three Amino Acid-Secreting Lactococcus lactis Strains. AU - Hernandez-Valdes, Jhonatan A.. AU - de Jong, Anne. AU - Kok, Jan. AU - Kuipers, Oscar P.. N1 - Copyright © 2020 Hernandez-Valdes et al.. PY - 2020/4/16. Y1 - 2020/4/16. N2 - Three Lactococcus lactis strains with the ability to secrete various amino acids (leucine, isoleucine, methionine, valine, glutamic acid, and histidine) were sequenced in order to identify the mechanisms involved in the secretion. Amino acids contribute to flavor formation; therefore, bacterial strains with this ability are relevant for the food industry.. AB - Three Lactococcus lactis strains with the ability to secrete various amino acids (leucine, isoleucine, methionine, valine, glutamic acid, and histidine) were sequenced in order to identify the mechanisms involved in the secretion. Amino acids contribute to flavor formation; therefore, bacterial strains with this ability are relevant for the food industry.. KW - ...
https://www.rug.nl/research/portal/publications/draft-genome-sequences-of-three-amino-acidsecreting-lactococcus-lactis-strains
6-Phosphogluconate dehydrogenase from Lactococcus lactis: a role for arginine residues in binding substrate and coenzyme |...6-Phosphogluconate dehydrogenase from Lactococcus lactis: a role for arginine residues in binding substrate and coenzyme |...
A gene encoding 6-phosphogluconate dehydrogenase (6-PGDH, EC 1.1.1.44) was identified from the homofermentative lactic acid bacterium Lactococcus lactis, by complementation of Escherichia coli mutants. The cloned gene was then expressed to high levels in E. coli and the protein purified for kinetic analysis. The enzyme had a Km for 6-phosphogluconate of 15.4±1.4 µM and for NADP of 1.9±0.2 µM at pH 7.5. Sequence comparison of the L. lactis 6-PGDH with the corresponding enzyme derived from the pathogenic protozoan Trypanosoma brucei and sheep liver revealed the substrate-binding residues to be identical in all three species, although the three coenzyme-binding pockets differed slightly. A totally conserved arginine residue (Arg-447), believed to bind the 6-phosphate of substrate, was mutated to lysine, aspartate, alanine or tryptophan. In each case enzyme activity was lost, confirming an essential role for this residue on activity. A second arginine (Arg-34), believed to be critical in binding ...
http://www.biochemj.org/content/338/1/55
Two different pathways for D-xylose metabolism and the effect of xylose concentration on the yield coefficient of L-lactate in...
TY - JOUR. T1 - Two different pathways for D-xylose metabolism and the effect of xylose concentration on the yield coefficient of L-lactate in mixed-acid fermentation by the lactic acid bacterium Lactococcus lactis IO-1. AU - Tanaka, K.. AU - Komiyama, A.. AU - Sonomoto, K.. AU - Ishizaki, A.. AU - Hall, S.. AU - Stanbury, P.. PY - 2002/10. Y1 - 2002/10. N2 - In lactic acid bacteria, pentoses are metabolized via the phosphoketolase pathway, which catalyzes the cleavage of D-xylulose-5-phosphate to equimolar amounts of glyceraldehyde 3-phosphate and acetylphosphate. Hence the yield coefficient of lactate from pentose does not exceed 1.0 mol/mol, while that of Lactococcus lactis IO-1(JCM7638) at high D-xylose concentrations often exceeds the theoretical value. This suggests that, in addition to the phosphoketolase pathway, L. lactis IO-1 may possess another metabolic pathway that produces only lactic acid from xylose. In the present study, the metabolism of xylose in L. lactis IO-1 was deduced ...
https://kyushu-u.pure.elsevier.com/en/publications/two-different-pathways-for-d-xylose-metabolism-and-the-effect-of-
Cloning and expression of a novel lactococcal aggregation factor from Lactococcus lactis subsp. lactis BGKP1 | BMC Microbiology...Cloning and expression of a novel lactococcal aggregation factor from Lactococcus lactis subsp. lactis BGKP1 | BMC Microbiology...
The aggregation phenomenon in lactococci has been studied for more than thirty years. It was found to be directly associated with a sex factor and lactose plasmid co-integration event [1] or duplication of the cell wall spanning (CWS) domain of PrtP proteinase [2]. Lactose plasmid conjugation in Lactococcus lactis 712 and in the related strains C2 and ML3, frequently involves plasmid co-integration with a sex factor. Moreover, this phenomenon is often associated with a cell aggregation phenotype and high frequency transfer ability [3-5]. The lactococcal sex factor exists integrated in the chromosome [6], although it can be excised as a closed circular form and lost from the cell [1]. Deletion and over-expression experiments confirmed that CluA is the only sex factor component responsible for aggregation in L. lactis. This 136 kDa surface-bound protein, encoded by the chromosomally located sex factor of Lactococcus lactis subsp. cremoris MG1363, is associated with cell aggregation linked to ...
https://bmcmicrobiol.biomedcentral.com/articles/10.1186/1471-2180-11-265
Recombinant porcine epidermal growth factor-secreting Lactococcus lactis promotes the growth performance of early-weaned...Recombinant porcine epidermal growth factor-secreting Lactococcus lactis promotes the growth performance of early-weaned...
Epidermal growth factor (EGF) is an important growth factor in regulation of cell proliferation, differentiation, survival and apoptosis. Studies showed that food-grade Lactococcus lactis (L. lactis) and NICE expression system have superior performance in exogenous protein expression. This study aimed to construct and express porcine EGF (pEGF), and use L. lactis as vehicle for producing and delivering pEGF. Furthermore, investigating biological activity of pEGF and exploring applications feasibility of combination effects of L. lactis and pEGF on early weaned piglets production. A recombinant Lactococcus lactis which produced and secreted pEGF at 1000 ng/ml in culture supernatant was generated. Secreted pEGF was a fully biologically active protein, as demonstrated by its capacity to stimulate L929 mouse fibroblast cell line proliferation in vitro. For in vivo study, forty piglets were randomly allocated to control, antibiotic control, empty vector-expressing L. lactis (LL-EV) and pEGF-secreting L.
https://bmcvetres.biomedcentral.com/articles/10.1186/s12917-014-0171-1
Expression of two Listeria monocytogenes antigens (P60 and LLO) in Lactococcus lactis and examination for use as live vaccine...Expression of two Listeria monocytogenes antigens (P60 and LLO) in Lactococcus lactis and examination for use as live vaccine...
Listeria monocytogenes is a food-borne intracellular pathogen that mainly infects pregnant and immunocompromised individuals. The pore-forming haemolysin listeriolysin O (LLO), the main virulence factor of Listeria monocytogenes, allows bacteria to escape from the harsh environment of the phagosome to the cytoplasm of the infected cell. This leads to processing of bacterial antigens predominantly through the cytosolic MHC class I presentation pathway. We previously engineered the food-grade bacterium Lactococcus lactis to express LLO and demonstrated an LLO-specific CD8+ response upon immunization of mice with the engineered L. lactis vaccine strains. In the present work, we examined the immune response and protective efficacy of an L. lactis strain co-expressing LLO and a truncated form of the listerial P60 antigen (tP60). Oral immunization revealed no significant protection against listeriosis with L. lactis expressing LLO, tP60 or the combined LLO/tP60. In contrast, intraperitoneal vaccination
https://www.microbiologyresearch.org/content/journal/jmm/10.1099/jmm.0.018770-0
Immunopathological evaluation of recombinant mycobacterial antigen Hsp65 expressed in Lactococcus lactis as a novel vaccine...Immunopathological evaluation of recombinant mycobacterial antigen Hsp65 expressed in Lactococcus lactis as a novel vaccine...
Bovine tuberculosis (TBB) is a zoonotic disease distributed worldwide and is of great importance for public health and the livestock industry. Several experimental vaccines against this disease have been evaluated in recent years, yielding varying results. An example is the Bacillus Calmette-Guérin (BCG) vaccine, which has been used extensively in humans and tested in cattle showing mixed results related to protection (0-80%) against Mycobacterium bovis. In this study, we used the food-grade bacterium Lactococcus lactis as an expression system for production of mycobacterial protein Hsp65. For this purpose, the construction of a replicable plasmid in strain NZ9000 L. lactis (pVElepr) was conducted, which expressed the Mycobacterium leprae Hsp65 antigen, and was recognized by traded anti-Hsp65 antibodies. The strain NZ9000-pVElepr was applied to calves that were negative to tuberculin test and the immune response was monitored. The results showed that immune response was not significantly increased in
http://ijvr.shirazu.ac.ir/article_4222.html
UniProt/SWISS-PROT: KITH LACLA
ID KITH_LACLA Reviewed; 189 AA. AC Q9CHX5; DT 31-OCT-2003, integrated into UniProtKB/Swiss-Prot. DT 01-JUN-2001, sequence version 1. DT 07-JUN-2017, entry version 82. DE RecName: Full=Thymidine kinase {ECO:0000255,HAMAP-Rule:MF_00124}; DE EC=2.7.1.21 {ECO:0000255,HAMAP-Rule:MF_00124}; GN Name=tdk {ECO:0000255,HAMAP-Rule:MF_00124}; OrderedLocusNames=LL0593; GN ORFNames=L184871; OS Lactococcus lactis subsp. lactis (strain IL1403) (Streptococcus OS lactis). OC Bacteria; Firmicutes; Bacilli; Lactobacillales; Streptococcaceae; OC Lactococcus. OX NCBI_TaxID=272623; RN [1] RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=IL1403; RX PubMed=11337471; DOI=10.1101/gr.GR-1697R; RA Bolotin A., Wincker P., Mauger S., Jaillon O., Malarme K., RA Weissenbach J., Ehrlich S.D., Sorokin A.; RT The complete genome sequence of the lactic acid bacterium Lactococcus RT lactis ssp. lactis IL1403.; RL Genome Res. 11:731-753(2001). CC -!- CATALYTIC ACTIVITY: ATP + thymidine = ADP + thymidine 5- CC phosphate. ...
http://www.genome.jp/dbget-bin/www_bget?sp:KITH_LACLA
The Transcriptional and Gene Regulatory Network of Lactococcus lactis MG1363 during Growth in Milk - Danish National Research...The Transcriptional and Gene Regulatory Network of Lactococcus lactis MG1363 during Growth in Milk - Danish National Research...
In the present study we examine the changes in the expression of genes of Lactococcus lactis subspecies cremoris MG1363 during growth in milk. To reveal which specific classes of genes (pathways, operons, regulons, COGs) are important, we performed a transcriptome time series experiment. Global analysis of gene expression over time showed that L. lactis adapted quickly to the environmental changes. Using upstream sequences of genes with correlated gene expression profiles, we uncovered a substantial number of putative DNA binding motifs that may be relevant for L. lactis fermentative growth in milk. All available novel and literature-derived data were integrated into network reconstruction building blocks, which were used to reconstruct and visualize the L. lactis gene regulatory network. This network enables easy mining in the chrono-transcriptomics data. A freely available website at http://milkts.molgenrug.nl gives full access to all transcriptome data, to the reconstructed network and to the ...
http://www.forskningsdatabasen.dk/catalog/236422795
Further elucidation of galactose utilization in Lactococcus lactis MG1363Further elucidation of galactose utilization in Lactococcus lactis MG1363
Since the 1970s, galactose metabolism in Lactococcus lactis has been in debate. Different studies led to diverse outcomes making it difficult to conclude whether galactose uptake was PEP- or ATP-dependent and decide what the exact connection was between galactose and lactose uptake and metabolism. It was shown that some Lactococcus strains possess two galactose-specific systems - a permease and a PTS, even if they lack the lactose utilization plasmid, proving that a lactose-independent PTSGal exists. However, the PTSGal transporter was never identified. Here, with the help of transcriptome analyses and genetic knock-out mutants, we reveal the identities of two low-affinity galactose PTSs. A novel plant-niche-related PTS component Llmg_0963 forming a hybrid transporter Llmg_0963PtcBA and a glucose/mannose-specific PTS are shown to be involved in galactose transport in L. lactis MG1363 ...
https://cora.ucc.ie/handle/10468/6656?show=full
Genotype-phenotype matching analysis of 38 Lactococcus lactis strains using random forest methodsGenotype-phenotype matching analysis of 38 Lactococcus lactis strains using random forest methods
BACKGROUND: Lactococcus lactis is used in dairy food fermentation and for the efficient production of industrially relevant enzymes. The genome content and different phenotypes have been determined for multiple L. lactis strains in order to understand intra-species genotype and phenotype diversity and annotate gene functions. In this study, we identified relations between gene presence and a collection of 207 phenotypes across 38 L. lactis strains of dairy and plant origin. Gene occurrence and phenotype data were used in an iterative gene selection procedure, based on the Random Forest algorithm, to identify genotype-phenotype relations. RESULTS: A total of 1388 gene-phenotype relations were found, of which some confirmed known gene-phenotype relations, such as the importance of arabinose utilization genes only for strains of plant origin. We also identified a gene cluster related to growth on melibiose, a plant disaccharide; this cluster is present only in melibiose-positive strains and can be ...
https://repository.ubn.ru.nl/handle/2066/118486
The First Clinical Use of a Recombinant Lactococcus lactis Expressing Human Papillomavirus Type 16 E7 Oncogene Oral Vaccine: A...The First Clinical Use of a Recombinant Lactococcus lactis Expressing Human Papillomavirus Type 16 E7 Oncogene Oral Vaccine: A...
Figure 2. HPV-16 E7-specific serum IgG antibody (1, top) and E7-specific vaginal IgA antibody (2, bottom) levels by placebo groups [cohorts 1 (A, left); 1 × 109 CFU/mL dose, cohorts 2 (B, center); 5 × 109 CFU/mL dose, and cohorts 3 (C, right); 1 × 1010 CFU/mL dose] and vaccine group [cohorts 4 (A, left); 1 × 109 CFU/mL dose, cohorts 5 (B, center); 5 × 109 CFU/mL dose, and cohorts 6 (C, right); 1 × 1010 CFU/mL dose] measured by ELISA at a serum dilution of 1:100 using goat anti-human IgG H&L (HRP) antibody and at a vaginal fluid dilution of 1:10 using goat anti-human IgA alpha chain (HRP) antibody, respectively. Results are expressed with 95% CIs for the following time points: prevaccination (day 0), days 30, 60, 90, and 240. The absorbance of each well was measured at 450 nm. Bars represent the mean ± standard error value of each group. Statistically significant differences are denoted by an asterisk between T60 and T30, T90, and T240 of vaccine groups (*, P ≤ 0.0001; **, P ≤ 0.001; ...
https://mct.aacrjournals.org/content/19/2/717.figures-only
Mucosal Immunization with Recombinant Lactococcus lactis | Springer for Research & DevelopmentMucosal Immunization with Recombinant Lactococcus lactis | Springer for Research & Development
The United States National Institutes of Health has recently pointed out that despite many years of intensive vaccine research and the development of sophisticated effective vaccines, thousands of...
https://rd.springer.com/chapter/10.1007/978-3-662-07548-7_5
Production, active staining and gas chromatography assay analysis of recombinant aminopeptidase P from Lactococcus lactis ssp....Production, active staining and gas chromatography assay analysis of recombinant aminopeptidase P from Lactococcus lactis ssp....
The aminopeptidase P (PepP, EC 3.4.11.9) gene from Lactococcus lactis ssp. lactis DSM 20481 was cloned, sequenced and expressed recombinantly in E. coli BL21 (DE3) for the first time. PepP is involved in the hydrolysis of proline-rich proteins and, thus, is important for the debittering of protein hydrolysates. For accurate determination of PepP activity, a novel gas chromatographic assay was established. The release of L-leucine during the hydrolysis of L-leucine-L-proline-L-proline (LPP) was examined for determination of PepP activity. Sufficient recombinant PepP production was achieved via bioreactor cultivation at 16 °C, resulting in PepP activity of 90 μkatLPP Lculture-1. After automated chromatographic purification by His-tag affinity chromatography followed by desalting, PepP activity of 73.8 μkatLPP Lculture-1 was achieved. This was approximately 700-fold higher compared to the purified native PepP produced by Lactococcus lactis ssp. lactis NCDO 763 as described in literature. The molecular
https://amb-express.springeropen.com/articles/10.1186/2191-0855-2-39/figures/6
Antimicrobial effect of selected lactic acid bacteria against microorganisms with decarboxylase activityAntimicrobial effect of selected lactic acid bacteria against microorganisms with decarboxylase activity
The main purpose of this study was to evaluate the antimicrobial activity of twenty-one bacteriocinogenic lactic acid bacteria (12 strains of Lactococcus lactis subsp. lactis, 4 strains of Lactobacillus gasseri, 3 strains of Lb. helveticus and 2 strains of Lb. acidophilus, LAB) against 28 Staphylococcus and 33 Enterococcus strains able to produce tyramine, putrescine, 2-phenylethylamine and cadaverine. The antimicrobial activity of cell-free supernatants (CFS) from tested LAB was examined by an agar-well diffusion assay. Nine out of twenty-one strains (33%) showed the inhibitory effect on tested enterococci and staphylococci, namely 9 strains of Lactococcus lactis subsp. lactis. The diameters of inhibition zones ranged between 7 mm and 14 mm. The biggest diameter of 14 mm inhibition was obtained with the CFSs from strains CCDM 670 and CCDM 731 on Enterococcus sp. E16 and E28. The cell-free supernatants from Lactococcus lactis subsp. lactis CCDM 71 and from Lactococcus lactis subsp. lactis CCDM ...
https://publikace.k.utb.cz/handle/10563/1007434?locale-attribute=en
Genomic features of Lactococcus lactis IO-1, a lactic acid bacterium that utilizes xylose and produces high levels of L-lactic...
TY - JOUR. T1 - Genomic features of Lactococcus lactis IO-1, a lactic acid bacterium that utilizes xylose and produces high levels of L-lactic acid. AU - Shimizu-Kadota, Mariko. AU - Kato, Hiroaki. AU - Shiwa, Yuh. AU - Oshima, Kenshiro. AU - Machii, Miki. AU - Araya-Kojima, Tomoko. AU - Zendo, Takeshi. AU - Hattori, Masahira. AU - Sonomoto, Kenji. AU - Yoshikawa, Hirofumi. PY - 2013. Y1 - 2013. N2 - Lactococcus lactis IO-1 (JCM7638) produces L-lactic acid predominantly when grown at high xylose concentrations, and its utilization is highly desired in the green plastics industry. Therefore it is worthwhile studying its genomic traits. In this study, we focused on (i) genes of possible horizontal transfer derivation (prophages, the nisin-sucrose transposon, and several restrictionmodification systems), and (ii) genes for the synthetic pathways of amino acids and vitamins in the IO-1 genome. In view of the results of this analysis, we consider their meanings in strain IO-1.. AB - Lactococcus ...
https://waseda.pure.elsevier.com/en/publications/genomic-features-of-lactococcus-lactis-io-1-a-lactic-acid-bacteri
Lactococcus lactis - WikipediaLactococcus lactis - Wikipedia
Lactococcus lactis is a Gram-positive bacterium used extensively in the production of buttermilk and cheese, but has also become famous as the first genetically modified organism to be used alive for the treatment of human disease. L. lactis cells are cocci that group in pairs and short chains, and, depending on growth conditions, appear ovoid with a typical length of 0.5 - 1.5 µm. L. lactis does not produce spores (nonsporulating) and are not motile (nonmotile). They have a homofermentative metabolism, meaning they produce lactic acid from sugars. Theyve aso been reported to produce exclusive L-(+)-lactic acid. However, reported D-(−)-lactic acid can be produced when cultured at low pH. The capability to produce lactic acid is one of the reasons why L. lactis is one of the most important microorganisms in the dairy industry. Based on its history in food fermentation, L. lactis has generally recognized as safe (GRAS) status with few case reports of being an opportunistic pathogen. L. lactis ...
https://en.wikipedia.org/wiki/Lactococcus_lactis
Production, secretion and purification of a correctly folded staphylococcal antigen in Lactococcus lactis | Microbial Cell...Production, secretion and purification of a correctly folded staphylococcal antigen in Lactococcus lactis | Microbial Cell...
Lactococcus lactis, a Gram-positive lactic acid bacterium and a classical starter for the manufacture of cheeses, can be used as a cell factory to produce proteins of interest [1-6]. As a long known innocuous, Generally Recognised As Safe (GRAS) food-grade species [7], L. lactis is an interesting host to produce proteins of medical interest [2, 4, 5]. Compared to Escherichia coli, the advantage of L. lactis is that it does not produce endotoxin (lipopolysaccharide) [2, 4, 5] which has to be removed from protein preparations before medical use [8]. In contrast to Bacillus subtilis, L. lactis secretes only one major endogenous protein, Usp45, and no proteases [9]: a strategy combining production and secretion of heterologous proteins in L. lactis is thus interesting as it facilitates protein purification from the culture medium [4]. As secreted heterologous proteins can be degraded by the lactococcal surface protease HtrA, protein yield can be improved by the use of a mutant strain devoid of ...
https://microbialcellfactories.biomedcentral.com/articles/10.1186/s12934-015-0271-z
Difference between revisions of IGEM:Caltech/2008/Project/Vitamins - OpenWetWareDifference between revisions of IGEM:Caltech/2008/Project/Vitamins - OpenWetWare
Although folate is naturally produced in E.coli, the folate biosynthesis pathway in the bacteria Lactococcus lactis has been more heavily characterized and studied. There are six major enzymes involved in folate synthesis, which, in L.lactis, are contained in five genes: folB, folKE, folP, folC, and folA[1]. The first four, which we have chosen to focus on, are located in a gene cluster approximately 4.4kb long. Weve chosen not to focus on folA for the time being because folA encodes an enzyme which turns one form of folate (dihydrofolate) into another form of folate(tetrahydrofolate). Since our assay would detect both types of folate as part of the total folate production, folA was not a prime target for overexpression of folate. In previous studies, this folate gene cluster has been successfully transformed into the folate-consuming bacteria L.gasseri, turning the bacteria in to folate-producers[3]. Therefore, we have chosen to also use the folate operon from L.lactis, which also offers the ...
https://openwetware.org/wiki/?title=IGEM:Caltech/2008/Project/Vitamins&diff=227150&oldid=225678
Difference between revisions of IGEM:Caltech/2008/Project/Vitamins - OpenWetWareDifference between revisions of IGEM:Caltech/2008/Project/Vitamins - OpenWetWare
Although folate is naturally produced in E.coli, the folate biosynthesis pathway in the bacteria Lactococcus lactis has been more heavily characterized and studied. There are six major enzymes involved in folate synthesis, which, in L.lactis, are contained in five genes: folB, folKE, folP, folC, and folA[1]. The first four, which we have chosen to focus on, are located in a gene cluster approximately 4.4kb long. Weve chosen not to focus on folA for the time being because folA encodes an enzyme which turns one form of folate (dihydrofolate) into another form of folate(tetrahydrofolate). Since our assay would detect both types of folate as part of the total folate production, folA was not a prime target for overexpression of folate. In previous studies, this folate gene cluster has been successfully transformed into the folate-consuming bacteria L.gasseri, turning the bacteria in to folate-producers[3]. Therefore, we have chosen to also use the folate operon from L.lactis, which also offers the ...
https://openwetware.org/wiki/?title=IGEM:Caltech/2008/Project/Vitamins&diff=227150&oldid=227143
LactococcusLactococcus
How was I to know?. Lactococcus lactis is part of my culture and heritage. Does that make me uncultured for not knowing about this cultures importance, or, to be honest, not knowing about it at all? L.lactis is the microbe which changes milk into many varieties of cheese.. The Assembly in my fair state, Wisconsin, has passed a bill designating Lactococcus lactis as our official state microbe. Since our State Senate has yet to vote, Lactococcus is lobbying hard. How many other bacterium have their own home pages? (The Lactococcus lactis Home Page). At the risk of sounding like a disloyal cheesehead, I am a bit upset about the designation. The fact that my state has been made a laughing stock by the media doesnt faze me. Living in a place where people don large wedges of foam cheese on their heads, I dont fret much about the states image.. No, I am upset because the candidate that I am backing for state designation is languishing in some committee over in Madison. How can our state go ...
http://www.thesuitcaselady.com/lactococcus/
Forzea - Probiotic Night Cream
Our Probiotic Night Cream is virtually a miracle treatment for damaged skin and has several high-end ingredients that have been developed over years of testing and research. What is Probiotic Technology? Probiotics are bacteria that are in some form beneficial to us. Our Probiotic Night Cream contains a biotechnologically obtained suspension from the bacterium Lactococcus lactis that contains cytoplasm and cell wall fragments which are highly effective in helping the skin to heal and to remain healthy and younger appearing. Some benefits of our Probiotic Technology are: • Induces production of key ingredients of skin quality and differentiation resulting in healthier skin. • Improves keratinocyte cohesion resulting in a more robust skin barrier that protects the skin. • Increases the production of antimicrobial peptides which fight harmful bacteria. • Promotes skin renewal (old cells being replaced with new and healthier cells). In addition to our Probiotic Technology, weve also added ...
http://forzea.com/probicr.htm
Cell & Plant Physiology Laboratory - First biochemical characterization of a Cu+-ATPase from plant
Until now, data on HMA1, HMA6 and HMA8 functions were based on indirect measures; the absence of biochemical data can be explained by the difficulty to produce high amounts of these membrane proteins in an active form. The CEA-PM project dedicated to the screening of alternative expression system for membrane proteins showed that the bacteria Lactococcus lactis is particularly suitable for the production of plant membrane proteins, including the HMAs [2, 3] ...
https://www.lpcv.fr/en/Pages/News/2012/Daphne-Seigneurin-Berny.aspx
IAR Gandhinagar Vaccine Development JRF Vacancy
Applications are invited for the position of Junior Research Fellow on SERB sponsored project entitled Evaluation of immunogenicity and protective efficacy of bivalent vaccine candidate against Shigella and Salmonella using food grade bacteria Lactococcus lactis under the supervision of Dr. Priti Desai, Institute of Advanced Research, Koba Institutional Area, Koba, Gandhinagar-382 007. ...
https://www.helpbiotech.co.in/2018/12/iar-gandhinagar-vaccine-development-jrf.html
ldhX - L-lactate dehydrogenase 3 - Lactococcus lactis subsp. lactis (strain IL1403) - ldhX gene & proteinldhX - L-lactate dehydrogenase 3 - Lactococcus lactis subsp. lactis (strain IL1403) - ldhX gene & protein
p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.,/p> ,p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.,/p> ,p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).,/p> ,p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x,sup>64,/sup> + x,sup>4,/sup> + x,sup>3,/sup> + x + 1. The algorithm is described in the ISO 3309 standard. ,/p> ,p class=publication>Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.,br /> ,strong>Cyclic redundancy and other checksums,/strong>,br /> ,a href=http://www.nrbook.com/b/bookcpdf.php>Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993),/a>),/p> Checksum:i ...
http://www.uniprot.org/uniprot/Q9CGG8
KEGG PATHWAY: Ubiquinone and other terpenoid-quinone biosynthesis - Lactococcus lactis subsp. cremoris SK11
Ubiquinone (UQ), also called coenzyme Q, and plastoquinone (PQ) are electron carriers in oxidative phosphorylation and photosynthesis, respectively. The quinoid nucleus of ubiquinone is derived from the shikimate pathway; 4-hydroxybenzoate is directly formed from chorismate in bacteria, while it can be formed from either chorismate or tyrosine in yeast. The following biosynthesis of terpenoid moiety involves reactions of prenylation, decarboxylation, and three hydroxylations alternating with three methylations. The order of these reactions are somewhat different between bacteria and yeast. Phylloquinone (vitamin K1), menaquinone (vitamin K2), and tocopherol (vitamin E) are fat-soluble vitamins. Phylloquinone is a compound present in all photosynthetic plants serving as a cofactor for photosystem I-mediated electron transport. Menaquinone is an obligatory component of the electron-transfer pathway in bacteria ...
http://www.genome.jp/kegg-bin/show_pathway?llc00130
Overall control of nitrogen metabolism in Lactococcus lactis by CodY, and possible models for CodY regulation in FirmicutesOverall control of nitrogen metabolism in Lactococcus lactis by CodY, and possible models for CodY regulation in Firmicutes
CodY, a pleiotropic transcriptional regulator conserved in low G+C species of Gram-positive bacteria, was previously described to be the central regulator of proteolysis in Lactococcus lactis. In this study, over 100 potential CodY targets were identified by DNA-microarray analysis. Complementary tr …
https://pubmed.ncbi.nlm.nih.gov/16339935/
A Genome-Scale Integration and Analysis of Lactococcus lactis Translation Data - InfoscienceA Genome-Scale Integration and Analysis of Lactococcus lactis Translation Data - Infoscience
Protein synthesis is a template polymerization process composed by three main steps: initiation, elongation, and termination. During translation, ribosomes are engaged into polysomes whose size is used for the quantitative characterization of translatome. However, simultaneous transcription and translation in the bacterial cytosol complicates the analysis of translatome data. We established a procedure for robust estimation of the ribosomal density in hundreds of genes from Lactococcus lactis polysome size measurements. We used a mechanistic model of translation to integrate the information about the ribosomal density and for the first time we estimated the protein synthesis rate for each gene and identified the rate limiting steps. Contrary to conventional considerations, we find significant number of genes to be elongation limited. This number increases during stress conditions compared to optimal growth and proteins synthesized at maximum rate are predominantly elongation limited. Consistent with
https://infoscience.epfl.ch/record/189837
Antibody responses to two new Lactococcus lactis -produced recombinant Pfs48/45 and Pfs230 proteins increase with age in...Antibody responses to two new Lactococcus lactis -produced recombinant Pfs48/45 and Pfs230 proteins increase with age in...
Recent advances in malaria control efforts have led to an increased number of national malaria control programmes implementing pre-elimination measures and demonstrated the need to develop new tools to track and control malaria transmission. Key to understanding transmission is monitoring the prevalence and immune response against the sexual stages of the parasite, known as gametocytes, which are responsible for transmission. Sexual-stage specific antigens, Pfs230 and Pfs48/45, have been identified and shown to be targets for transmission blocking antibodies, but they have been difficult to produce recombinantly in the absence of a fusion partner. Regions of Pfs48/45 and Pfs230 known to contain transmission blocking epitopes, 6C and C0, respectively, were produced in a Lactococcus lactis expression system and used in enzyme linked immunosorbent assays to determine the seroreactivity of 95 malaria patients living in the Central Region of Ghana. Pfs48/45.6C and Pfs230.C0 were successfully produced in L.
https://malariajournal.biomedcentral.com/articles/10.1186/s12936-017-1955-0/figures/3
Lactococcus Lactis Probiotics - Lyophilized Probiotics SupplementsLactococcus Lactis Probiotics - Lyophilized Probiotics Supplements
Get The Natural Lactococcus Lactis Probiotics Products Online From DBI360.com. We Provide The Best Lyophilized Vitamin Products & Probiotics Ingredients As Per Your Requirement.
https://www.dbi360.com/lactococcus-lactis-probiotics-lyophilized.html
Lactococcus lactis - WikipediaLactococcus lactis - Wikipedia
L. lactis is of crucial importance for manufacturing dairy products, such as buttermilk and cheeses. When L. lactis ssp. lactis is added to milk, the bacterium uses enzymes to produce energy molecules (ATP), from lactose. The byproduct of ATP energy production is lactic acid. The lactic acid produced by the bacterium curdles the milk that then separates to form curds, which are used to produce cheese.[11] Other uses that have been reported for this bacterium include the production of pickled vegetables, beer or wine, some breads, and other fermented foodstuffs, such as soymilk kefir, buttermilk, and others.[12] L. lactis is one of the best characterized low GC Gram positive bacteria with detailed knowledge on genetics, metabolism and biodiversity.[13][14]. L. lactis is mainly isolated from either the dairy environment or plant material.[15][16][17] Dairy isolates are suggested to have evolved from plant isolates through a process in which genes without benefit in the rich medium milk were either ...
https://en.m.wikipedia.org/wiki/Lactococcus_lactis
Citrate Metabolism - Beverage Fermentation - Buffalo Brewing BlogCitrate Metabolism - Beverage Fermentation - Buffalo Brewing Blog
Citrate is present in only low levels 0.15-0.2 in milk and O-type starters do not include the citrate utilizing Lc. lactis subsp. lactis biovar diacetylactis.
https://www.buffalobrewingstl.com/beverage-fermentation/citrate-metabolism.html
xseB - Exodeoxyribonuclease 7 small subunit - Lactococcus lactis subsp. cremoris (strain SK11) - xseB gene & proteinxseB - Exodeoxyribonuclease 7 small subunit - Lactococcus lactis subsp. cremoris (strain SK11) - xseB gene & protein
Bidirectionally degrades single-stranded DNA into large acid-insoluble oligonucleotides, which are then degraded further into small acid-soluble oligonucleotides.
http://www.uniprot.org/uniprot/Q030A1
Discrimination of lactic acid bacteria Enterococcus and Lactococcus by infrared spectroscopy and multivariate techniques -...Discrimination of lactic acid bacteria Enterococcus and Lactococcus by infrared spectroscopy and multivariate techniques -...
Raw milk is often described as a major source of lactic acid bacteria for indigenous lactic starter. These indigenous starters contribute to the sensorial quality of cheese. Raw milk, rich in Lactoccocus lactis may therefore be very interesting for the cheese making. Currently, the most commonly used methods to differentiate lactic acid bacteria, and particularly the closely related phenotypes Lactococcus and Enterococcus, are based on DNA sequencing, but the cost and time required for these analytical methods hinder their use for rapid screening of raw material. The present study therefore proposes a simple alternative method to identify and discriminate against Lactococcus and Enterococcus, at the genus, but also at the species level, that is based on collecting near infrared spectra directly from bacterial colonies in Petri dishes. The infrared spectra of 280 strains of Lactococcus and Enterococcus cultured on solid media were collected by using a spectrometer with a wavelength range of 908 ...
https://oatao.univ-toulouse.fr/25137/
Staff PublicationsStaff Publications
Background: Vitamin K2 (menaquinone, MK-n) is a lipid-soluble vitamin that functions as a carboxylase co-factor for maturation of proteins involved in many vital physiological processes in humans. Notably, long-chain vitamin K2 is produced by bacteria, including some species and strains belonging to the group of lactic acid bacteria (LAB) that play important roles in food fermentation processes. This study was performed to gain insights into the natural long-chain vitamin K2 production capacity of LAB and the factors influencing vitamin K2 production during cultivation, providing a basis for biotechnological production of vitamin K2 and in situ fortification of this vitamin in food products. Results: We observed that six selected Lactococcus lactis strains produced MK-5 to MK-10, with MK-8 and MK-9 as the major MK variant. Significant diversities between strains were observed in terms of specific concentrations and titres of vitamin K2. L. lactis ssp. cremoris MG1363 was selected for more ...
https://library.wur.nl/WebQuery/wurpubs?A320==Respiration
Systems solutions by lactic acid bacteria: from paradigms to practice | Microbial Cell Factories | Full TextSystems solutions by lactic acid bacteria: from paradigms to practice | Microbial Cell Factories | Full Text
One of the great achievements of the omics revolution is the development of high throughput functional genomics approaches. Notably transcriptomics studies have been instrumental in analyzing the response of LAB to different environments and over 100 papers have been published addressing a variety of stresses, growth conditions and culturing regimens. Among the most important practical discoveries was the finding that Lactococcus lactis and other LAB, when provided with the appropriate cofactors, such as heme, could use alternative electron acceptors and hence respire rather than ferment [82]. Following the first description of the use of molecular oxygen by Lactococcus lactis MG1363 [83], a variety of studies have followed that have been reviewed recently [84]. Transcriptional studies allowed to identify the genes involved in the use of molecular oxygen that resulted in a wide range of applications, notably faster growth and higher yield of starter cultures [85]. Biochemical studies confirmed ...
https://microbialcellfactories.biomedcentral.com/articles/10.1186/1475-2859-10-S1-S2
Lactococcus lactis (Streptococcus lactis) - CTValley Bio
Gram-positive sphere. Forms short chains. A common contaminant of milk products, causing souring. Excellent for classroom demonstrations. Incubate at 37°C. On brain heart infusion …
https://www.connecticutvalleybiological.com/individual-cultures/lactococcus-lactis-streptococcus-lactis.html
Viruses | Free Full-Text | Structural Aspects of the Interaction of Dairy Phages with Their Host BacteriaViruses | Free Full-Text | Structural Aspects of the Interaction of Dairy Phages with Their Host Bacteria
Knowledge of phage-host interactions at a fundamental level is central to the design of rational strategies for the development of phage-resistant strains that may be applied in industrial settings. Phages infecting lactic acid bacteria, in particular Lactococcus lactis and Streptococcus thermophilus, negatively impact on dairy fermentation processes with serious economic implications. In recent years a wealth of information on structural protein assembly and topology has become available relating to phages infecting Escherichia coli, Bacillus subtilis and Lactococcus lactis, which act as models for structural analyses of dairy phages. In this review, we explore the role of model tailed phages, such as T4 and SPP1, in advancing our knowledge regarding interactions between dairy phages and their hosts. Furthermore, the potential of currently investigated dairy phages to in turn serve as model systems for this particular group of phages is discussed.
http://mdpi.com/1999-4915/4/9/1410
Viruses | Free Full-Text | Structural Aspects of the Interaction of Dairy Phages with Their Host Bacteria | HTMLViruses | Free Full-Text | Structural Aspects of the Interaction of Dairy Phages with Their Host Bacteria | HTML
Knowledge of phage-host interactions at a fundamental level is central to the design of rational strategies for the development of phage-resistant strains that may be applied in industrial settings. Phages infecting lactic acid bacteria, in particular Lactococcus lactis and Streptococcus thermophilus, negatively impact on dairy fermentation processes with serious economic implications. In recent years a wealth of information on structural protein assembly and topology has become available relating to phages infecting Escherichia coli, Bacillus subtilis and Lactococcus lactis, which act as models for structural analyses of dairy phages. In this review, we explore the role of model tailed phages, such as T4 and SPP1, in advancing our knowledge regarding interactions between dairy phages and their hosts. Furthermore, the potential of currently investigated dairy phages to in turn serve as model systems for this particular group of phages is discussed.
http://www.mdpi.com/1999-4915/4/9/1410/htm
SWISS-MODEL Repository | P96790
SWISS-MODEL Repository entry for P96790 (KUP1_LACLM), Putative potassium transport system protein kup 1. Lactococcus lactis subsp cremoris (strain MG1363)
https://swissmodel.expasy.org/repository/uniprot/P96790
SWISS-MODEL Repository | A2RNR2
SWISS-MODEL Repository entry for A2RNR2 (RL333_LACLM), 50S ribosomal protein L33 3. Lactococcus lactis subsp cremoris (strain MG1363)
https://swissmodel.expasy.org/repository/uniprot/A2RNR2
Functions of starters in dairy fermentations. The importance and effectiveness of their antimicrobial mechanismsFunctions of starters in dairy fermentations. The importance and effectiveness of their antimicrobial mechanisms
The functions of starters in dairy fermentations and the importance of the antimicrobial agents produced by starters including undissociated lactic acid is discussed
https://www.dairyscience.info/index.php/cheese-starters/225-role-of-starters.html
Construction of specific erythromycin resistance mutations in the temperate lactococcal bacteriophage TP901-1 and their use in...Construction of specific erythromycin resistance mutations in the temperate lactococcal bacteriophage TP901-1 and their use in...
Construction of specific erythromycin resistance mutations in the temperate lactococcal bacteriophage TP901-1 and their use in studies of phage biology ...
https://food.ku.dk/english/staff/?pure=en%2Fpublications%2Fconstruction-of-specific-erythromycin-resistance-mutations-in-the-temperate-lactococcal-bacteriophage-tp9011-and-their-use-in-studies-of-phage-biology
Thymidylate synthase gene from Lactococcus lactis as a genetic marker: an alternative to antibiotic resistance genes. | Applied...Thymidylate synthase gene from Lactococcus lactis as a genetic marker: an alternative to antibiotic resistance genes. | Applied...
The potential of the thymidylate synthase thyA gene cloned from Lactococcus lactis subsp. lactis as a possible alternative selectable marker gene to antibiotic resistance markers has been examined. The thyA mutation is a recessive lethal one; thyA mutants cannot survive in environments containing low amounts of thymidine or thymine (such as Luria-Bertani medium) unless complemented by the thyA gene. The cloned thyA gene was strongly expressed in L. lactis subsp. lactis, Escherichia coli, Rhizobium meliloti, and a fluorescent Pseudomonas strain. In addition, when fused to a promoterless enteric lac operon, the thyA gene drove expression of the lac genes in a number of gram-negative bacteria. In transformation experiments with thyA mutants of E. coli and conjugation experiments with thyA mutants of R. meliloti, the lactococcal thyA gene permitted selection of transformants and transconjugants with the same efficiency as did genes for resistance to ampicillin, chloramphenicol, or tetracycline. ...
https://aem.asm.org/content/56/7/2164
Nisin - definition of nisin by The Free DictionaryNisin - definition of nisin by The Free Dictionary
Define nisin. nisin synonyms, nisin pronunciation, nisin translation, English dictionary definition of nisin. n. A bacteriocin produced by the bacterium Lactococcus lactis and used as a preservative in dairy products and other foods and in cosmetics
https://www.thefreedictionary.com/Nisin
Structure, Adsorption to Host, and Infection Mechanism of Virulent Lactococcal Phage p2 | Journal of VirologyStructure, Adsorption to Host, and Infection Mechanism of Virulent Lactococcal Phage p2 | Journal of Virology
We report here the complete EM structure of a lactococcal phage determined by single-particle analysis. Phage p2 is the flagship of the 936 group, which are the most prevalent lactococcal phages in industrial dairy fermentations worldwide. This phage is highly virulent and requires Ca2+ to infect specific L. lactis cells, which is in contrast with the less prevalent lactococcal phages belonging to the P335 subgroup II (e.g., TP901-1 and Tuc2009) (15).. The shape of p2 icosahedral capsid is comparable to that of other siphophages. The capsid pentons are, however, more prominent than those of phage TP901-1 but akin to those of coliphage HK97. In contrast, the size of the p2 portal protein (ORF-4) is among the smallest, particularly in comparison to those of phage TP901-1 and Bacillus phage SPP1 (Table 3). The two putative head completion proteins of p2 (ORF-8 and ORF-9) are comparable to those of phage SPP1. The fit of the SPP1 portal and head completion protein 1 (gp15) into the p2 connector EM ...
https://jvi.asm.org/content/87/22/12302?ijkey=5a467b90e4a35df20bbed5b914cccafdb088a509&keytype2=tf_ipsecsha
Control analysis as a tool to understand the formation of the las operon in Lactococcus lactis - DTU OrbitControl analysis as a tool to understand the formation of the las operon in Lactococcus lactis - DTU Orbit
In Lactococcus lactis the enzymes phosphofructokinase (PFK), pyruvate kinase (PK) and lactate dehydrogenase (LDH) are uniquely encoded in the las operon and we here apply Metabolic Control Analysis to study the role of this organisation. Earlier work showed that LDH at wildtype level has zero control on glycolysis and growth rate but high negative control on formate production. We find that PFK and PK have zero control on glycolysis and growth rate at the wildtype enzyme level but both enzymes exert strong positive control on the glycolytic flux at reduced activities. PK has high positive control on formate and acetate production, whereas PFK has no control on these fluxes. Decreased expression of the entire las operon resulted in a strong decrease in growth rate and the glycolytic flux; at 53% expression of the las operon the glycolytic flux was reduced to 44% and the flux control coefficient increased towards 3. Increased las expression resulted in a slight decrease in the glycolytic flux. At ...
http://orbit.dtu.dk/en/publications/control-analysis-as-a-tool-to-understand-the-formation-of-the-las-operon-in-lactococcus-lactis
Production of Human Papillomavirus Type 16 E7 Protein in Lactococcus lactis | Applied and Environmental MicrobiologyProduction of Human Papillomavirus Type 16 E7 Protein in Lactococcus lactis | Applied and Environmental Microbiology
The E7 protein of human papillomavirus type 16 was produced in Lactococcus lactis. Secretion allowed higher production yields than cytoplasmic production. In stationary phase, amounts of cytoplasmic E7 were reduced, while amounts of secreted E7 increased, suggesting a phase-dependent intracellular proteolysis. Fusion of E7 to the staphylococcal nuclease, a stable protein, resulted in a highly stable cytoplasmic protein. This work provides new candidates for development of viral screening systems and for oral vaccine against cervical cancer. ...
https://aem.asm.org/content/68/2/917.abstract
Probiotičke kulture Lactobacillus plantarum B i Lactococcus lactis subsp. lactis S1 u poboljšanju funkcionalnih svojstava sira...Probiotičke kulture Lactobacillus plantarum B i Lactococcus lactis subsp. lactis S1 u poboljšanju funkcionalnih svojstava sira...
Cheese in a sack is a specific Croatian indigenous semi hard cheese. The main characteristic of this cheese is ripening and keeping in sheeps clothing (Mišina), which gives it a typical and very appreciated, spicy flavor and aroma. Cheeses obtained by similar technology and ripening are also produced in Turkey (Tulum cheese), Lebanon (darfiyeh), Bosnia and Herzegovina (sack cheese), Montenegro (sack cheese) and eastern Algeria (bouhezza cheese). In Croatia, cheese in a sack has been produced traditionally for many years, without the use of commercial starter cultures, which can result in differences in taste, consistency and microbiological quality.Therefore, there is a growing trend of finding and selecting of new natural indigenous potential starter cultures from traditional products, which will be adapted to the growth in certain specific traditional products, and will have such metabolic capabilities that will significantly affect the quality and safety of products. The hypothesis is that ...
https://dr.nsk.hr/islandora/object/agr%3A472
Change from homo- to heterolactic fermentation by Streptococcus lactis resulting from glucose limitation in anaerobic chemostat...
Lactic streptococci, classically regarded as homolactic fermenters of glucose and lactose, became heterolactic when grown with limiting carbohydrate concentrations in a chemostat. At high dilution rates (D) with excess glucose present, about 95% of the fermented sugar was converted to l-lactate. However, as D was lowered and glucose became limiting, five of the six strains tested changed to a heterolactic fermentation such that at D = 0.1 h(-1) as little as 1% of the glucose was converted to l-lactate. The products formed after this phenotypic change in fermentation pattern were formate, acetate, and ethanol. The level of lactate dehydrogenase, which is dependent upon ketohexose diphosphate for activity, decreased as fermentation became heterolactic with Streptococcus lactis ML(3). Transfer of heterolactic cells from the chemostat to buffer containing glucose resulted in the nongrowing cells converting nearly 80% of the glucose to l-lactate, indicating that fine control of enzyme activity is an ...
https://www.meta.org/papers/change-from-homo--to-heterolactic-fermentation-by/108249
Protein balance of Lactococcus lactis in cheese production - WURProtein balance of Lactococcus lactis in cheese production - WUR
Lactic acid bacteria are used as starter cultures in dairy fermentations. Starter cultures employed in cheese manufacturing encounter many different environmental conditions during their production in starter-producing companies and during their application in the cheese manufacturing industry. To optimally perform, microorganisms have to continuously adapt to these changing conditions. Bacteria adjust their physiology to maximize their fitness to the condition they are in, at the cost of being less fit under different conditions. Microbial fitness under different conditions is determined by rate of adaptation including proteome degradation, production and renewal. Adaptation and turnover of the proteome is constrained by the availability of resources and/or cellular energy levels, which results in trade-offs during optimized growth followed by adaptation: the optimization of one trait usually correlates with decreased performance of another trait. In this project, we investigate, by proteomics, ...
https://www.wur.nl/en/project/Protein-balance-of-Lactococcus-lactis-in-cheese-production-1.htm
Get PDF - Proteolytic action of lactic streptococci. I. The action of mesophilic lactic streptococci on N constituents of milkGet PDF - Proteolytic action of lactic streptococci. I. The action of mesophilic lactic streptococci on N constituents of milk
Kikuchi, T.; Desmazeaud, M.; Bergere, J.L., 1973: Proteolytic action of lactic streptococci. I. The action of mesophilic lactic streptococci on N constituents of milk
https://eurekamag.com/research/000/173/000173024.php
The effect of immunoregulation of Streptococcus lactis L16 strain upon Staphylococcus aureus infection | BMC Microbiology |...The effect of immunoregulation of Streptococcus lactis L16 strain upon Staphylococcus aureus infection | BMC Microbiology |...
Staphylococcus aureus is an important pathogen that causes various infections in medical facilities. However, resistance to multiple drugs has made this infection difficult to manage. Thus, new therapeutic strategies are urgently needed to solve this worldwide public health problem. The Streptococcus lactis L16 strain was isolated from the fermented hot chili sauce. To explore whether it can be used as a protective agent against S. aureus infection, we designed a mouse model of S. aureus infection to evaluate the therapeutic potency of S. lactis. Mice were grouped into pre-(P) and post-(T) S. aureus infection groups following oral administration of S. lactis L16. The protection and treatment effects were assessed by examining body weight, internal organ weight, serum cytokines and intestinal secretory IgA alternations. Oral administration of the S. lactis L16 strain reduced the loss of body weight in mice post-infection and alleviated infection-induced hepatomegaly. In
https://bmcmicrobiol.biomedcentral.com/articles/10.1186/s12866-017-1038-y/figures/1
Bifidobacterium lactis - Nutrivee
B. lactis is a widely used probiotic and is considered a champion among the good bacteria. It has been reclassified by Masco et al. (2004) and has been found to be related to Bifidobacterium animalis at the sup specie level, B. animalis subsp. Lactis. B. lactis is a common inhabitant of the colon and intestines of humans and animals. The use of B. lactis in diets has shown beneficial effects in the treatment of various gluten-based enteropathies. Celiac disease is an autoimmune disorder which is aggravated by the consumption of gluten-based food products.. According to Lindfors et al. (2008), the oral intake of B. lactis based-probiotics protects the intestinal cells from the toxic effects of gluten enteropathies and inflammatory reactions. The regular consumption of B. lactis aids in controlling abnormal weight gain and diabetes in animal models. Stenman et al. (2014) administered a probiotic based on Bifidobacterium animalis ssp. Lactis 420 to mice, which showed positive results in ...
https://nutrivee.com/bifidobacterium-lactis/
Disruption and Analysis of the clpB, clpC, and clpE Genes in Lactococcus lactis: ClpE, a New Clp Family in Gram-Positive...
PubMed Central Canada (PMC Canada) provides free access to a stable and permanent online digital archive of full-text, peer-reviewed health and life sciences research publications. It builds on PubMed Central (PMC), the U.S. National Institutes of Health (NIH) free digital archive of biomedical and life sciences journal literature and is a member of the broader PMC International (PMCI) network of e-repositories.
http://pubmedcentralcanada.ca/pmcc/articles/PMC93619/
EC 2.4.1.216EC 2.4.1.216
Accepted name: trehalose 6-phosphate phosphorylase. Reaction: α,α-trehalose 6-phosphate + phosphate = glucose 6-phosphate + β-D-glucose 1-phosphate. Systematic name: α,α-trehalose 6-phosphate:phosphate β-D-glucosyltransferase. Comments: The enzyme from Lactococcus lactis is specific for trehalose 6-phosphate. Differs from EC 2.4.1.64, α,α-trehalose phosphorylase, in that trehalose is not a substrate.. Links to other databases: BRENDA, EXPASY, KEGG, Metacyc, CAS registry number: 403512-51-6. References:. 1. Andersson, U., Levander, F. and Radstrom, P. Trehalose 6-phosphate phosphorylase is part of a novel metabolic pathway for trehalose utilization in Lactococcus lactis. J. Biol. Chem. 276 (2001) 42707-42713. [PMID: 11553642]. ...
http://www.sbcs.qmul.ac.uk/iubmb/enzyme/EC2/4/1/216.html
Antibody responses to two new Lactococcus lactis-produced recombinant Pfs48/45 and Pfs230 proteins increase with age in malaria...Antibody responses to two new Lactococcus lactis-produced recombinant Pfs48/45 and Pfs230 proteins increase with age in malaria...
Recent advances in malaria control efforts have led to an increased number of national malaria control programmes implementing pre-elimination measures and demonstrated the need to develop new tools to track and control malaria transmission. Key to understanding transmission is monitoring the prevalence and immune response against the sexual stages of the parasite, known as gametocytes, which are responsible for transmission. Sexual-stage specific antigens, Pfs230 and Pfs48/45, have been identified and shown to be targets for transmission blocking antibodies, but they have been difficult to produce recombinantly in the absence of a fusion partner. Regions of Pfs48/45 and Pfs230 known to contain transmission blocking epitopes, 6C and C0, respectively, were produced in a Lactococcus lactis expression system and used in enzyme linked immunosorbent assays to determine the seroreactivity of 95 malaria patients living in the Central Region of Ghana. Pfs48/45.6C and Pfs230.C0 were successfully produced in L.
https://malariajournal.biomedcentral.com/articles/10.1186/s12936-017-1955-0
Heterologous production of human papillomavirus type-16 L1 protein by a lactic acid bacterium | BMC Research Notes | Full TextHeterologous production of human papillomavirus type-16 L1 protein by a lactic acid bacterium | BMC Research Notes | Full Text
The expression of vaccine antigens in lactic acid bacteria (LAB) is a safe and cost-effective alternative to traditional expression systems. In this study, we investigated i) the expression of Human papillomavirus type 16 (HPV-16) L1 major capsid protein in the model LAB Lactococcus lactis and ii) the ability of the resulting recombinant strain to produce either capsomer-or virus-like particles (VLPs). HPV-16 L1 gene was cloned into two vectors, pCYT and pSEC, designed for controlled intra- or extracellular heterologous expression in L. lactis, respectively. The capacity of L. lactis harboring either pCYT:L1 or pSEC:L1 plasmid to accumulate L1 in the cytoplasm and supernatant samples was confirmed by Western blot assays. Electron microscopy analysis suggests that, L1 protein produced by recombinant lactococci can self-assemble into structures morphologically similar to VLPs intracellularly. The presence of conformational epitopes on the L. lactis-derived VLPs was confirmed by ELISA using an anti-HPV16
https://bmcresnotes.biomedcentral.com/articles/10.1186/1756-0500-2-167/tables/1
Enhanced Nutty Flavor Formation in Cheddar Cheese Made with Malty Lact by M. E. Carunchia Whetstine, M. A. Drake et al.Enhanced Nutty Flavor Formation in Cheddar Cheese Made with Malty Lact by M. E. Carunchia Whetstine, M. A. Drake et al.
Carunchia Whetstine, M. E.; M. A. Drake, J. R Broadbent, and McMahon. 2006. Enhanced nutty flavor formation in Cheddar cheese made with a malty Lactococcus lactis adjunct culture. J. Dairy Sci. 89: 3277-3284. ...
https://digitalcommons.usu.edu/nfs_facpub/137/
prof.dr. M. (Michiel) Kleerebezem - WURprof.dr. M. (Michiel) Kleerebezem - WUR
His expertise centers around the molecular biology and physiology of bacteria, with a special focus on lactic acid bacteria, probiotics, and the intestinal microbiota. He is strongly involved in the genomics driven work related to understanding the molecular mechanisms of environmental adaptation in Lactobacillus plantarum and Lactococcus lactis, focusing on adaptation to the conditions encountered in the oro-gastrointestinal tract and conditions encountered during (industrial) dairy fermentation, respectively. In recent years he has expanded his field to the (post-genomic) molecular analysis of the mechanisms of communication between intestinal bacteria and the host mucosa. Michiel Kleerebezem has (co-) authored more than 200 peer-reviewed publications and is inventor on more than 25 patent applications ...
https://www.wur.nl/web/show/id=3614912/langid=13167723
Genomic Characterization of Dairy Associated Leuconostoc Species and D by Cyril A. Frantzen, Witold Kot et al.Genomic Characterization of Dairy Associated Leuconostoc Species and D by Cyril A. Frantzen, Witold Kot et al.
Undefined mesophilic mixed (DL-type) starter cultures are composed of predominantly Lactococcus lactis subspecies and 1-10% Leuconostoc spp. The composition of the Leuconostoc population in the starter culture ultimately affects the characteristics and the quality of the final product. The scientific basis for the taxonomy of dairy relevant leuconostocs can be traced back 50 years, and no documentation on the genomic diversity of leuconostocs in starter cultures exists. We present data on the Leuconostoc population in five DL-type starter cultures commonly used by the dairy industry. The analyses were performed using traditional cultivation methods, and further augmented by next-generation DNA sequencing methods. Bacterial counts for starter cultures cultivated on two different media, MRS and MPCA, revealed large differences in the relative abundance of leuconostocs. Most of the leuconostocs in two of the starter cultures were unable to grow on MRS, emphasizing the limitations of culture-based methods
https://digitalcommons.usu.edu/nfs_facpub/860/
Munin: Bacterial diversity of aMasi, a South African fermented milk product, determined by clone library and denaturing...Munin: Bacterial diversity of aMasi, a South African fermented milk product, determined by clone library and denaturing...
In the present study, we investigated the bacterial diversity of aMasi, a traditional South African fermented milk product, by 16S rRNA clone library and Denaturing Gradient Gel Electrophoresis (DGGE) analysis. Two hundred and eighty two clones from clone library were isolated and identified from aMasi, prepared from the milk of four cows from one herd in the EkuPindiseni Community, North West of Hluhluwe-iMfolozi Park in KwaZulu-Natal Province. The majority of the identified sequences corresponded to lactic acid bacteria (LAB), with the genus Lactococcus as major representative. The species Lactococcus lactis accounted for 179 of the identified clones. In addition, several species of Lactobacillus, Leuconostoc and Enterococcus were detected. Furthermore, several clones belonging to Acinetobacter, Aeromonas and genera within the Enterobacteriaceae were detected. It is important to note that human pathogens such as Klebsiella pneumoniae were identified in aMasi in the present study. Conversely, ...
https://munin.uit.no/handle/10037/5613
Cross-immunity and immune mimicry as mechanisms of resistance to the lantibiotic lacticin 3147. - PubMed - NCBICross-immunity and immune mimicry as mechanisms of resistance to the lantibiotic lacticin 3147. - PubMed - NCBI
PubMed comprises more than 30 million citations for biomedical literature from MEDLINE, life science journals, and online books. Citations may include links to full-text content from PubMed Central and publisher web sites.
https://www.ncbi.nlm.nih.gov/pubmed/19183281?dopt=Abstract
Characterization of the lactococcal conjugative element pRS01 using IS946-mediated mutagenesis | SpringerLinkCharacterization of the lactococcal conjugative element pRS01 using IS946-mediated mutagenesis | SpringerLink
Conjugation is a common mode of genetic transfer among the lactic acid bacteria. In an effort to exploit conjugation as a means of lactococcal strain development, we have characterized the transfer re
https://link.springer.com/article/10.1023%2FA%3A1009759525576
Get PDF - Production of proteolytic activity by selected strains of lactic streptococci in skim milk and a whey mediumGet PDF - Production of proteolytic activity by selected strains of lactic streptococci in skim milk and a whey medium
Vahora, G.Y.; Ernstrom, C.A.; Post, F.J., 1973: Production of proteolytic activity by selected strains of lactic streptococci in skim milk and a whey medium
https://eurekamag.com/research/027/215/027215339.php
Variability at gene and genome levels revealed different genomic lineages in Lactococcus garvieae | Archivio Istituzionale...Variability at gene and genome levels revealed different genomic lineages in Lactococcus garvieae | Archivio Istituzionale...
Lactococcus garvieae is a pathogen that causes septicemia in fish and serious damage to fish aquaculture worldwide, but recently has been isolated from human, ruminant, water sources and several food matrix. In the present work we monitored the population structure of L. garvieae strains, including isolates of dairy, fish, meat, vegetable and cereal origin. This bacterial collection was explored using molecular polyphasic approach comprising PCR-ribotyping, Rep and RAPD-PCR analyses and multilocus restriction typing (MLRT) carried out on six partial genes. This approach allowed high-resolution cluster analysis in which two major groups were distinguishable: one group included dairy isolates, the other group meat isolates. Unexpectedly, some strains coming from fish grouped with dairy isolates whereas the other with meat isolates. Likewise, strains isolated from vegetables allocated between the two main groups. These findings revealed high variability within the species at both gene and genome ...
https://air.unimi.it/handle/2434/204642
Structure and Conformational Dynamics of the Domain 5 RNA Hairpin of a Bacterial Group II Intron Revealed by Solution Nuclear...Structure and Conformational Dynamics of the Domain 5 RNA Hairpin of a Bacterial Group II Intron Revealed by Solution Nuclear...
Structure and Conformational Dynamics of the Domain 5 RNA Hairpin of a Bacterial Group II Intron Revealed by Solution Nuclear Magnetic Resonance and Molecular Dynamics ...
https://www.research-collection.ethz.ch/handle/20.500.11850/74686
Diamond Publications - Search Results
Abstract: Multidrug efflux pumps present a challenge to the treatment of bacterial infections, making it vitally important to understand their mechanism of action. Here, we investigate the nature of substrate binding within Lactococcus lactis LmrP, a prototypical multidrug transporter of the major facilitator superfamily. We determined the crystal structure of LmrP in a ligand-bound outward-open state and observed an embedded lipid in the binding cavity of LmrP, an observation supported by native mass spectrometry analyses. Molecular dynamics simulations suggest that the anionic lipid stabilizes the observed ligand-bound structure. Mutants engineered to disrupt binding of the embedded lipid display reduced transport of some, but not all, antibiotic substrates. Our results suggest that a lipid within the binding cavity could provide a malleable hydrophobic component that allows adaptation to the presence of different substrates, helping to explain the broad specificity of this protein and ...
https://publications.diamond.ac.uk/pubman/searchpublicationsadvanced?advancedsearch=1&authorId=98377&publicationStates%5B4%5D.selected=true
The FAIRDOMHubThe FAIRDOMHub
Im a modeller, specialized in kinetic modeling of biochemical networks. My focus in the SysMO-LAB consortium is on creating models of Lactococcus lactis glycolysis and couple this to other related lactic acid bacteria like Streptococcus pyogenes and Enterococcus faecalis. Besides kinetic modeling, Im also interested in combining various modeling techniques (genome-scale modeling, qualitative modeling). ...
https://fairdomhub.org/tags/32
The FAIRDOMHubThe FAIRDOMHub
Im a modeller, specialized in kinetic modeling of biochemical networks. My focus in the SysMO-LAB consortium is on creating models of Lactococcus lactis glycolysis and couple this to other related lactic acid bacteria like Streptococcus pyogenes and Enterococcus faecalis. Besides kinetic modeling, Im also interested in combining various modeling techniques (genome-scale modeling, qualitative modeling). ...
https://seek.sysmo-db.org/tags/32
Wild Dairy Fermentation - Gianaclis CaldwellWild Dairy Fermentation - Gianaclis Caldwell
Excerpt from my article for Fermentation Magazine, access the full article here. One of the raw milk cheese I used to make for Pholia Farm. A Romano style goat, aged 1 year. Long before packets of freeze-dried cultures for milk existed, there were dairy ferments. Cheese, yogurt, and kefir are probably second only to beer…
https://gianacliscaldwell.com/2021/01/20/wild-dairy-fermentation/?shared=email&msg=fail
Articles - LISBP Laboratoire dIngénierie des Systèmes Biologiques et des Procédés
Vermassen A, Dordet-Frisoni E, de LaFoye A, Micheau P, Laroute V, Leroy S, Talon R. 2016. Adaptation of Staphylococcus xylosus to nutrients and osmotic stress in a salted meat model. Front Microbiol. Feb 7;87.. Morin M, Ropers D, Letisse F, Laguerre S, Portais J-C, Enjalbert B, Cocaign-Bousquet M. 2016. The post-transcriptional regulatory system CSR controls the balance of metabolic pools in upper glycolysis of Escherichia coli. Mol Microbiol ; doi: 10.1111/mmi.13343. Castelain M., Duviau M-P, Canette A, Schmitz P, Loubière P, Cocaign-Bousquet M, Piard J-C, Mercier-Bonin M. 2016. The nanomechanical properties of Lactococcus lactis pili are conditioned by the polymerized backbone pilin. PLoS ONE 11(3): e0152053. doi:10.1371/journal.pone.0152053. Esquerré T, Turlan C, Bouvier M, Carpousis AJ, Girbal L, Cocaign-Bousquet M. 2016. The Csr system regulates genome-wide mRNA stability and transcription and thus gene expression in Escherichia coli. Sci Rep. 2016 Apr 26;6:25057. doi: ...
http://www.lisbp.fr/fr/la_recherche/equipe_ead4/production_scientifique/articles.html
TCDB » SEARCHTCDB » SEARCH
2.A.105 The Mitochondrial Pyruvate Carrier (MPC) Family. The transport of pyruvate, the end product of glycolysis, into mitochondria is an essential process that provides the organelle with a major oxidative fuel. Herzig et al. (2012) reported that MPC is a heterocomplex formed by two members of the MPC family that are conserved from yeast to mammals. Members of the MPC family are in the inner mitochondrial membrane, and yeast mutants lacking MPC proteins show severe defects in mitochondrial pyruvate uptake. Coexpression of mouse MPC1 and MPC2 in Lactococcus lactis promoted transport of pyruvate across the membrane (Herzig et al., 2012). Yeast MPC proteins with an odd number of transmembrane segments and a matrix-exposed N-terminus are imported by the carrier pathway, using the receptor Tom70, small TIM chaperones, and the TIM22 complex. The TIM9.10 complex chaperones MPC proteins through the mitochondrial intermembrane space using conserved hydrophobic motifs that are also required for the ...
http://www.tcdb.org/search/result.php?tc=2.A.105
The Ordinary Buffet - KbeautyCafe Philippines
Introducing The Ordinary Buffet, a multi-peptide serum that targets multiple signs of aging - dryness, fine lines, wrinkles, dullness, and uneven texture. The formula combines a comprehensive array of studied techonologies to combat aging. The technologies are Matrixyl™ 3000 peptide complex (with palmitoyl tetrapeptide-7), Matrixyl™ synthe6™ peptide complex (with palmitoyl tripeptide-38), SYN™-AKE peptide complex (with dipeptide diaminobutyroyl benzylamide diacetate), Relistase peptide complex (with acetylarginyltryptophyl diphenylglycine), ARGIRELOX™ peptide complex (with acetyl hexapeptide-8, pentapeptide-18), Probiotic complex (with lactococcus lactis lysate), in a base of 11 skin-friendly amino acids and multiple hyaluronic acid complexes. The total concentration of these technologies in the formula by weight is a whopping 25.1%!. Start incorporating anti-aging serums as early as in your 20s!. Note from the The Ordinary: It is best to not use Peptides in the same routine as the ...
https://kbeautycafe.com.ph/product/the-ordinary-buffet/?page&product=the-ordinary-buffet&post_type=product&add_to_wishlist=15575
The Ordinary | Buffet - 30mlThe Ordinary | Buffet - 30ml
This formula combines a comprehensive array of studied technologies to target multiple signs of ageing at once. The technologies are Matrixyl 3,000 peptide complex (with palmitoyl tetrapeptide-7), Matrixyl Synthe6 peptide complex (with palmitoyl tripeptide-38), Syn-Ake peptide complex (with dipeptide diaminobutyroyl benzylamide diacetate), Relistase peptide complex (with acetylarginyltryptophyl diphenylglycine), Argirelox peptide complex (with acetyl hexapeptide-8, pentapeptide-18), Probiotic complex (with lactococcus lactis lysate), in a base of 11 skin-friendly amino acids and multiple hyaluronic acid complexes. The total concentration of these technologies in the formula by weight is 25.1%. (References are made to third-party trademarks in this description. All such trademarks are owned by their respective owners. Neither The Ordinary nor DECIEM is affiliated with these trademarks or their owners.) Note: The name of this formula was inspired by some brands referring to
http://theordinary.com/product/rdn-buffet-30ml
Search Results
Numerous significant hits to DNA polymerase I sequences; e.g. residues 3-284 are 38% similar to (X98575) DNA-dependent DNA polymerase of Anaerocellum thermophilum; residues 3-286 are 34% similar to (U78771) DNA polymerase I (PolI) of Lactococcus lactis; and residues 2-285 are 32% similar to DP01_STRPN ...
http://stdgen.northwestern.edu/cgi-bin/gene_id_search.cgi?dbname=uure&gene_id=UU414
Viral Movie Provides Clues to Infection Mechanism - Scientific AmericanViral Movie Provides Clues to Infection Mechanism - Scientific American
Latest news and features on science issues that matter including earth, environment, and space. Get your science news from the most trusted source!
https://www.scientificamerican.com/article/viral-movie-provides-clue/
Pre GI: Gene
General Information: Bifidobacterium animalis subsp. lactis (strain DSM 10140 / JCM 10602 / LMG 18314) is an anaerobic Gram-positive lactic acid bacterium commonly found in the guts of healthy humans and has been identified in the infant gut biota, particularly in ileal, fecal, and mucosal samples. Some strains of B. animalis subsp. lactis are able to survive in the GIT, to adhere to human epithelial cells in vitro, to modify fecal flora, to modulate the host immune response, or to prevent microbial gastroenteritis and colitis. ...
http://pregi.bi.up.ac.za/pre_gi_gene.php?source_accession=NC_012815&gi_accession=NC_012815:23000
Bio-purification of plant proteins - NIZO - consortia - Herwig BachmannBio-purification of plant proteins - NIZO - consortia - Herwig Bachmann
The bio-purification of plant protein consortium led by NIZO, will be aimed to develop strategies to remove off-flavours of plant ingredients.
https://www.nizo.com/bio-purification/
di sini bermula: Diari Jantung Abid: umur sebulan..simptom heart failure mula kelihatandi sini bermula: Diari Jantung Abid: umur sebulan..simptom heart failure mula kelihatan
semasa abid berusia 2 minggu, kami membawanya ke ppukm untuk 1st appointment di klinik paediatrik (kardiologi)..kali ni kami jumpa dengan doktor bilqis (pakar jantung kanak-kanak)..di awal pertemuan doktor memberitahu ohh inilah parent abid ye..doktor bilqis ni sangat tegas orangnya tapi sangat baik..soalan dia kena kita jawab straight tak boleh bunga-bunga konar sana konar sini..kecut perut sokmo kalu nok jawab soalan dia huhu...dari hasil pemeriksaan terhadap abid, doktor bilqis sahkan abid mempunyai 5 masalah pada jantungnya..terduduk seketika sebaik saja doktor bilqis explain kepada kami..sebelum ni masa dalam kandungan kami diberitahu yang abid hanya mengalami masalah vsd sahaja..doktor bilqis terangkan yang dalam kandungan kita tak dapat nak lihat 100% kerana baby dalam kandungan kita tak boleh lihat dengan jelas keseluruhannya..dari perbincangan tu doktor bagitau plan rawatan untuk abid..memang tak da jalan lain kecuali pembedahan dan sejujurnya kami memang dah bersedia mental fizikal ...
http://az-ila.blogspot.com/2016/03/diari-jantung-abid-umur-sebulansimptom.html
Immunomodulatory Properties of Bacterium-Like Particles Obtained From Immunobiotic Lactobacilli: Prospects for Their Use as...Immunomodulatory Properties of Bacterium-Like Particles Obtained From Immunobiotic Lactobacilli: Prospects for Their Use as...
Non-viable lactic acid bacteria (LAB) have been proposed as antigen delivery platforms called bacterium-like particles (BLPs). Most studies have been performed with Lactococcus lactis-derived BLPs where multiple antigens were attached to the peptidoglycan surface and used to successfully induce specific immune responses. It is well-established that the immunomodulatory properties of LAB are strain...
https://medworm.com/761473387/immunomodulatory-properties-of-bacterium-like-particles-obtained-from-immunobiotic-lactobacilli-pro/
Lactococcus lactisLactococcus lactis
Lactococcus_lactis Lactococcus lactis uses Kok J, Buist G, Zomer AL, van Hijum SA, Kuipers OP (2005). "Comparative and ... Lactococcus lactis is of crucial importance for manufacturing dairy products, such as buttermilk and cheeses. When L. lactis ... Nakamura S, Morimoto YV, Kudo S (2015). "A lactose fermentation product produced by Lactococcus lactis subsp. lactis, acetate, ... L. lactis subsp. lactis (formerly Streptococcus lactis) is used in the early stages for the production of many cheeses, ...
https://en.wikipedia.org/wiki/Lactococcus_lactis
MatzoonMatzoon
Lactococcus lactis ssp. cremoris was found to be a dominant bacterial strain producing polysaccharides that impart the ...
https://en.wikipedia.org/wiki/Matzoon
Extracellular polymeric substanceExtracellular polymeric substance
The exopolysaccharides of some strains of lactic acid bacteria, e.g., Lactococcus lactis subsp. cremoris, contribute a ...
https://en.wikipedia.org/wiki/Extracellular_polymeric_substance
BiovarBiovar
jugurti Lactococcus lactis subsp. lactis bv. diacetylactis Marinilabilia salmonicolor biovar Agarovorans Mesorhizobium ciceri ...
https://en.wikipedia.org/wiki/Biovar
State microbeState microbe
Lactococcus lactis was proposed as the State Microbe because of its crucial contribution to the cheese industry in Wisconsin. ... The proposed AB 556 simply stated that Lactococcus lactis is the State Microbe and should be included in the Wisconsin Blue ... "2009 Assembly Bill 556" (PDF). "2017 Wisconsin Dairy Data" (PDF). "Lactococcus lactis Wisconsin State Microbe". advanced.bact. ... Lactococcus lactis is vital for manufacturing cheeses such as Cheddar, Colby, cottage cheese, cream cheese, Camembert, ...
https://en.wikipedia.org/wiki/State_microbe
Microbial drug deliveryMicrobial drug delivery
Steidler, Lothar; Rottiers, Pieter (2006-08-01). "Therapeutic Drug Delivery by Genetically Modified Lactococcus lactis". Annals ... Lactococcus lactis and Bacteroides ovatus. The usage of recombinant microbes (i.e. microorganisms designed to contain DNA from ... The L. lactis mechanism of microbial drug delivery described in the 2006 study of Braat et al. uses a form of recombinant L. ... L. lactis is considered by the Food and Drug Administration (FDA) to be generally recognized as safe (GRAS), as it is commonly ...
https://en.wikipedia.org/wiki/Microbial_drug_delivery
Toxin-antitoxin systemToxin-antitoxin system
... an abortive infection mechanism from Lactococcus lactis". Applied and Environmental Microbiology. 64 (12): 4748-56. doi:10.1128 ...
https://en.wikipedia.org/wiki/Toxin-antitoxin_system
LactococcusLactococcus
bovis Lactococcus hircilactis Lactococcus lactis L. lactis subsp. cremoris L. lactis subsp. hordniae L. lactis subsp. lactis L ... lactis subsp. tructae Lactococcus laudensis Lactococcus nasutitermitis Lactococcus piscium Lactococcus plantarum Lactococcus ... They are: Lactococcus chungangensis Lactococcus formosensis Lactococcus fujiensis Lactococcus garvieae L. garvieae subsp. ... Special interest is placed on the study of L. lactis subsp. lactis and L. lactis subsp. cremoris, as they are the strains used ...
https://en.wikipedia.org/wiki/Lactococcus
Genetically modified bacteriaGenetically modified bacteria
"Recombinant Lactococcus lactis fails to secrete bovine chymosine". Bioengineered. 5 (6): 363-370. doi:10.4161/bioe.36327. PMC ...
https://en.wikipedia.org/wiki/Genetically_modified_bacteria
ProbioticProbiotic
Lactococcus lactis, and Leuconostoc species. Buttermilk contains either Lactococcus lactis or L. bulgaricus. Other acidic ... Lactococcus lactis LA 103 and Streptococcus thermophillus LA 104 and reducing intestinal discomfort pursuant to Article 13(5) ... Pao cai contains L. pentosus, L. plantarum , Leuconostoc mesenteroides , L. brevis, L. lactis, and L. fermentum. A list of many ...
https://en.wikipedia.org/wiki/Probiotic
Human milk microbiomeHuman milk microbiome
Beasley SS, Saris PE (August 2004). "Nisin-producing Lactococcus lactis strains isolated from human milk". Applied and ... and Lactococcus species, and later composed primarily of Veillonella, Prevotella, Leptotrichia, Lactobacillus, Streptococcus, ...
https://en.wikipedia.org/wiki/Human_milk_microbiome
Laceyella sacchariLaceyella sacchari
"Recombinant expression of Laceyella sacchari thermitase in Lactococcus lactis". Protein Expression and Purification. 92 (2): ...
https://en.wikipedia.org/wiki/Laceyella_sacchari
Xaa-Pro dipeptidyl-peptidaseXaa-Pro dipeptidyl-peptidase
Zevaco C, Monnet V, Gripon JC (1990). "Intracellular X-prolyl dipeptidyl peptidase from Lactococcus lactis spp. lactis: ... "Identification of the active site serine of the X-prolyl dipeptidyl aminopeptidase from Lactococcus lactis". FEBS Letters. 314 ... Ile The intracellular enzyme from Lactococcus lactis (190-kDa) is the type example of peptidase family S15. ... lactis DSM7290". Applied Microbiology and Biotechnology. 40 (1): 82-9. doi:10.1007/bf00170433. PMID 7765315. Habibi-Najafi MB, ...
https://en.wikipedia.org/wiki/Xaa-Pro_dipeptidyl-peptidase
Fermented milk productsFermented milk products
Streptococcus lactis has been renamed to Lactococcus lactis subsp. lactis Food portal List of dairy products List of yogurt- ... "Transfer of Streptococcus lactis and related streptococci to the. genus Lactococcus gen. nov". Syst. Appl. Microbiol. 6 (2): ... Lactococcus, and Leuconostoc. The fermentation process increases the shelf life of the product while enhancing its taste and ...
https://en.wikipedia.org/wiki/Fermented_milk_products
Cottage cheeseCottage cheese
A bacterial culture that produces lactic acid (Lactococcus lactis ssp. lactis or L. lactis ssp. cremoris strains such as are ... a small amount of low CO2-producing citrate-fermenting lactococci or leuconostoc bacterial strains are added to the starter mix ...
https://en.wikipedia.org/wiki/Cottage_cheese
PyrG leaderPyrG leader
Jørgensen CM, Hammer K, Martinussen J (2003). "CTP limitation increases expression of CTP synthase in Lactococcus lactis". J ...
https://en.wikipedia.org/wiki/PyrG_leader
Streptomyces tendaeStreptomyces tendae
"Secretion of Streptomyces tendae antifungal protein 1 by Lactococcus lactis". Brazilian Journal of Medical and Biological ...
https://en.wikipedia.org/wiki/Streptomyces_tendae
Bacterial translationBacterial translation
"Lactococcus lactis YfiA is necessary and sufficient for ribosome dimerization". Molecular Microbiology. 91 (2): 394-407. doi: ... Well-known coding regions that do not have AUG initiation codons are those of lacI (GUG) and lacA (UUG) in the E. coli lac ...
https://en.wikipedia.org/wiki/Bacterial_translation
KefirKefir
bulgaricus, Lactobacillus helveticus, Lactobacillus kefiranofaciens, Lactococcus lactis, and Leuconostoc species. Lactobacilli ... Kluyveromyces lactis, and Saccharomyces fragilis, as well as strains of yeast that do not metabolize lactose, including ...
https://en.wikipedia.org/wiki/Kefir
Major intrinsic proteinsMajor intrinsic proteins
A hypothetical protein from the pepX region of Lactococcus lactis. MIP family channels consist of homotetramers (e.g., GlpF of ...
https://en.wikipedia.org/wiki/Major_intrinsic_proteins
LLHol familyLLHol family
The Putative Lactococcus lactis Holin (LLHol) Family (TC# 1.E.44) consists of just a few proteins from Lactococcus lactis ... The Putative Lactococcus lactis Holin (LLHol) Family", which is licensed in a way that permits reuse under the Creative Commons ... "1.E.44 The Putative Lactococcus lactis Holin (LLHol) Family". TCDB. Retrieved 2016-03-29. Portal: Biology As of this edit, this ...
https://en.wikipedia.org/wiki/LLHol_family
Streptococcus mutansStreptococcus mutans
These genes possibly originated from Lactococcus lactis and S. gallolyticus, respectively. Another instance of lateral gene ...
https://en.wikipedia.org/wiki/Streptococcus_mutans
Branched-chain amino acid aminotransferaseBranched-chain amino acid aminotransferase
In contrast, Lactococcus lactis BCAT is a homodimer similar to the mammalian isoforms. Each subunit of the L. lactis BCAT is ... A study of BCAT from Lactococcus lactis by Yvon, Chambellon et al., found the ideal conditions for the bacterial isozyme as ... García-Cayuela T, Gómez de Cadiñanos LP, Peláez C, Requena T (October 2012). "Expression in Lactococcus lactis of functional ... isolated from Lactococcus lactis subsp. cremoris NCDO 763". Applied and Environmental Microbiology. 66 (2): 571-7. Bibcode: ...
https://en.wikipedia.org/wiki/Branched-chain_amino_acid_aminotransferase
Goat cheeseGoat cheese
Some examples of starters are Lactococcus Lactis, Lactis, Lactococcus Lactis subsp. cremoris, and Streptococcus thermophilus. ... "Effects of added Lactobacillus acidophilus and Bifidobacterium lactis probiotics on the quality characteristics of goat ricotta ...
https://en.wikipedia.org/wiki/Goat_cheese
Phage r1t holin familyPhage r1t holin family
The Lactococcus lactis Phage r1t Holin (r1t Holin) Family (TC# 1.E.18) is a family of putative pore-forming proteins that ... The Lactococcus lactis phage r1t genome includes two adjacent genes, orf48 and orf49, which encode Orf48 (TC# 1.E.18.1.1; 75 ... Portal: Biology As of this edit, this article uses content from "1.E.18 The Lactococcus lactis Phage r1t Holin (r1t Holin) ... "1.E.18 The Lactococcus lactis Phage r1t Holin (r1t Holin) Family". Transporter Classification Database. Retrieved 2016-03-28. ...
https://en.wikipedia.org/wiki/Phage_r1t_holin_family
ChangestatChangestat
The problem of choosing the correct acceleration of dilution has been studied with Escherichia coli and Lactococcus lactis ... "Multi-omics approach to study the growth efficiency and amino acid metabolism in Lactococcus lactis at various specific growth ... "Steady state growth space study of Lactococcus lactis in D-stat cultures". Antonie van Leeuwenhoek. 96 (4): 487-96. doi:10.1007 ...
https://en.wikipedia.org/wiki/Changestat
Carbohydrate Structure DatabaseCarbohydrate Structure Database
"Cell surface of Lactococcus lactis is covered by a protective polysaccharide pellicle". Journal of Biological Chemistry. 285 ( ...
https://en.wikipedia.org/wiki/Carbohydrate_Structure_Database
BhaturaBhatura
"Use of Lactococcus lactis to enrich sourdough bread with g-aminobutyric acid". International Journal of Food Sciences and ...
https://en.wikipedia.org/wiki/Bhatura
Galactose-6-phosphate isomeraseGalactose-6-phosphate isomerase
... and nucleotide sequence of the tagatose 6-phosphate pathway gene cluster of the lactose operon of Lactococcus lactis". J. Biol ... "Characterization of the lactose-specific enzymes of the phosphotransferase system in Lactococcus lactis". J. Biol. Chem. 265 ( ...
https://en.wikipedia.org/wiki/Galactose-6-phosphate_isomerase
LactocepinLactocepin
Visser S, Robben AJ, Slangen CJ (1991). "Specificity of a cell-envelope-located proteinase (PIII-type) from Lactococcus lactis ... "Diversity of cell envelope proteinase specificity among strains of Lactococcus lactis and its relationship to charge ... to hydrolyse hemoglobin and oxidized insulin B chain This enzyme is associated with the cell envelope of Lactococcus lactis and ...
https://en.wikipedia.org/wiki/Lactocepin
List of homing endonuclease cutting sitesList of homing endonuclease cutting sites
Shearman C, Godon JJ, Gasson M (July 1996). "Splicing of a group II intron in a functional transfer gene of Lactococcus lactis ... "Splicing of a group II intron involved in the conjugative transfer of pRS01 in lactococci". J Bacteriol. 178 (12): 3531-8. doi: ...
https://en.wikipedia.org/wiki/List_of_homing_endonuclease_cutting_sites
ActiviaActivia
... and Lactococcus lactis. Some experts say that there is evidence that probiotic strains reduce diarrhea, irritable bowel ... While indicating Bifidobacterium lactis, the package does not list that Activia also contains strains commonly found in Yogurt ... Bifidus Digestivum and Bifidobacterium Lactis. Danone launched Activia in France in 1987 under the "Bio" brand name. The claims ...
https://en.wikipedia.org/wiki/Activia
ButtermilkButtermilk
... and then inoculated with a culture of Lactococcus lactis or Lactobacillus bulgaricus plus Leuconostoc citrovorum to simulate ...
https://en.wikipedia.org/wiki/Buttermilk
Curd (India)Curd (India)
... and may include Lactococcus lactis, Streptococcus diacetylactis, Streptococcus cremoris, Lactobacillus delbrueckii subsp. ...
https://en.wikipedia.org/wiki/Curd_(India)
PhiU53 holin familyPhiU53 holin family
... origin of replication and modular structure of the Lactococcus lactis lytic bacteriophage sk1". Molecular Microbiology 26 (1): ...
https://en.wikipedia.org/wiki/PhiU53_holin_family
Dairy productDairy product
... mainly Lactococcus lactis and other bacterial cultures and yeasts Soured cream and crème fraîche Cultured buttermilk resembling ... and possible addition of the bacteria Streptococcus lactis and Leuconostoc citrovorum. Milk can be broken down into several ...
https://en.wikipedia.org/wiki/Dairy_product
Sour creamSour cream
The LAB that are known for producing the aromas in sour cream are Lactococcus lactis ssp. lactis biovar diacetyllactis. ... that are utilized to allow the fermentation of sour cream production are Lactococcus lactis subsp latic or Lactococcus lactis ...
https://en.wikipedia.org/wiki/Sour_cream
Jens NielsenJens Nielsen
Lactococcus lactis, Streptomyces coelicolor, Aspergillus oryzae, Aspergillus niger, Penicillium chrysogenum, and Pichia ...
https://en.wikipedia.org/wiki/Jens_Nielsen
Cyclic di-AMPCyclic di-AMP
... synthesis is inhibited by the GImM I154F mutation in the lactococcus lactis bacterium. GImM is the ... AMP synthesis by the diadenylate cyclase CdaA is modulated by the peptidoglycan biosynthesis enzyme GlmM in Lactococcus lactis ...
https://en.wikipedia.org/wiki/Cyclic_di-AMP
MilkMilk
Another milk with Lactococcus lactis bacteria cultures ("cultured buttermilk") often is used in cooking to replace the ... which also kills the naturally occurring Lactococcus bacteria. Commercially sold milk commonly has vitamin D added to it to ...
https://en.wikipedia.org/wiki/Milk
Crème fraîcheCrème fraîche
The culture is made up of a mix of bacteria including, Lactococcus species L. cremoris, L. lactis, and L. lactis biovar ...
https://en.wikipedia.org/wiki/Crème_fraîche
Tagatose-bisphosphate aldolaseTagatose-bisphosphate aldolase
... and nucleotide sequence of the tagatose 6-phosphate pathway gene cluster of the lactose operon of Lactococcus lactis". J. Biol ...
https://en.wikipedia.org/wiki/Tagatose-bisphosphate_aldolase
PHOSIDAPHOSIDA
... various organisms ranging from eukaryotes such as human and yeast to bacteria such as Escherichia coli and Lactococcus lactis. ...
https://en.wikipedia.org/wiki/PHOSIDA
Trehalose 6-phosphate phosphorylaseTrehalose 6-phosphate phosphorylase
"Trehalose-6-phosphate phosphorylase is part of a novel metabolic pathway for trehalose utilization in Lactococcus lactis". J. ...
https://en.wikipedia.org/wiki/Trehalose_6-phosphate_phosphorylase
GranadaeneGranadaene
The cyl genes has been cloned in Lactococcus lactis (a non-hemolytic non-pigmented Gram-positive bacterium) and the expression ... and cytoxicity to Lactococcus lactis. Proving that the expression of the genes of the cyl operon is sufficient for Granadaene ... "Feed Insects as a Reservoir of Granadaene-Producing Lactococci". Front. Microbiol. 13: Art 848490. doi:10.3389/fmicb. ...
https://en.wikipedia.org/wiki/Granadaene
CodY protein familyCodY protein family
The CodY protein also has a repressor effect on many genes in Lactococcus lactis during growth in milk. Slack FJ, Serror P, ... "Pleiotropic transcriptional repressor CodY senses the intracellular pool of branched-chain amino acids in Lactococcus lactis". ...
https://en.wikipedia.org/wiki/CodY_protein_family
ATP-binding cassette transporterATP-binding cassette transporter
... protein sequence homology and has overlapping substrate specificities with the MDR-ABC transporter LmrA from Lactococcus lactis ...
https://en.wikipedia.org/wiki/ATP-binding_cassette_transporter
KimchiKimchi
spicheri, Lactococcus carnosum, Lc. gelidum, Lc. lactis, Leuconostoc carnosum, Ln. citreum, Ln. gasicomitatum, Ln. gelidum, Ln ... lactis, Ln. mesenteroides, Serratia marcescens, Weissella cibaria, W. confusa, W. kandleri, W. kimchii. W. koreensis, and W. ...
https://en.wikipedia.org/wiki/Kimchi
Streptococcus thermophilusStreptococcus thermophilus
... a strain of Lactococcus lactis and a strain of S. thermophilus. These bacteria are chosen because they produce ... L. lactis produces cheese with higher moisture levels compared to other reduced-fat cheeses; S. thermophilus produces cheese ... It was concluded that applying both L. lactis and S. thermophilus strains would create higher-quality reduced-fat cheese with ...
https://en.wikipedia.org/wiki/Streptococcus_thermophilus
Microbial symbiosis and immunityMicrobial symbiosis and immunity
... produced by Lactococcus lactis. Nisin A inhibits methicillin-resistant S. aureus by binding to the precursor to bacterial cell ...
https://en.wikipedia.org/wiki/Microbial_symbiosis_and_immunity
ŽinčicaŽinčica
... is fermented by the following Lactic acid bacteria: Lactobacillus casei, Lactobacillus plantarum, Lactococcus lactis ...
https://en.wikipedia.org/wiki/Žinčica
Blue cheeseBlue cheese
... research has shown that other microbiota which are relatives of Lactococcus lactis, Enterococcus faecalis, Lactobacillus ... Then, acidification occurs: a starter culture, such as Streptococcus lactis, is added in order to change lactose to lactic acid ... and Kluyveromyces lactis and its non-sporulating form Candida sphaerica. Similarly to other kinds of blue cheeses, Roquefort's ...
https://en.wikipedia.org/wiki/Blue_cheese
FilmjölkFilmjölk
Arla's fil culture contains Lactococcus lactis subsp. lactis, Lactococcus lactis subsp. cremoris, Lactococcus lactis biovar. ... It is made by fermenting cow's milk with a variety of bacteria from the species Lactococcus lactis and Leuconostoc ... Fil culture is a variety of bacterium from the species Lactococcus lactis and Leuconostoc mesenteroides, e.g., ... and Verum Hälsofil which contains Lactococcus lactis L1A in quantities of at least 10 billion live bacteria per deciliter. ...
https://en.wikipedia.org/wiki/Filmjölk
Lactis-leu-phe leader RNA motifLactis-leu-phe leader RNA motif
Leu/phe leaders of this structure are known only in the species Lactococcus lactis, and is essentially the same as a previously ... Page for leu/phe leader RNA from Lactococcus lactis at Rfam v t e (Articles lacking reliable references from November 2021, All ... The leu/phe-leader RNA motif (also the lactis-leu/phe-leader motif) is a conserved RNA structure identified by bioinformatics. ...
https://en.wikipedia.org/wiki/Lactis-leu-phe_leader_RNA_motif
Resistance-nodulation-cell division superfamilyResistance-nodulation-cell division superfamily
... a new efflux protein conferring multiple antibiotic resistance in Lactococcus lactis and Escherichia coli". Antimicrobial ... It is expressed in L. lactis, E. coli and various other bacteria. Unlike other RND proteins Mdt(A) contains a putative ATP- ...
https://en.wikipedia.org/wiki/Resistance-nodulation-cell_division_superfamily
SCOP 1.65: Species: Lactococcus lactisSCOP 1.65: Species: Lactococcus lactis
Timeline for Species Lactococcus lactis [TaxId:1358] from b.113.1.1 DNA repair protein MutM (Fpg): *Species Lactococcus lactis ... Species Lactococcus lactis [TaxId:1358] from b.113.1.1 DNA repair protein MutM (Fpg) appears in SCOP 1.67. *Species Lactococcus ... PDB entries in Species: Lactococcus lactis:. *Domain(s) for 1kfv: *. Domain d1kfva2: 1kfv A:1-131 [75887]. Other proteins in ... Lineage for Species: Lactococcus lactis. *Root: SCOP 1.65 *. Class b: All beta proteins [48724] (126 folds). ...
http://scop.berkeley.edu/sunid=81617&ver=1.65
Assessment of the feed additive consisting of Lactococcus lactis NCIMB 30117 for all animal species for the renewal of its...Assessment of the feed additive consisting of Lactococcus lactis NCIMB 30117 for all animal species for the renewal of its...
EFSA was asked to deliver a scientific opinion on the assessment of the application for renewal of Lactococcus lactis NCIMB ... Assessment of the feed additive consisting of Lactococcus lactis NCIMB 30117 for all animal species for the renewal of its ... Assessment of the feed additive consisting of Lactococcus lactis NCIMB 30117 for all animal species… ... Technological additives, silage additive, Lactococcus lactis NCIMB 30117, safety, efficacy, QPS, renewal ...
https://www.efsa.europa.eu/pl/efsajournal/pub/7243
DailyMed - PLEO POLY OM- lactococcus lactis immunoserum rabbit, streptococcus pyogenes immunoserum rabbit, streptococcus...DailyMed - PLEO POLY OM- lactococcus lactis immunoserum rabbit, streptococcus pyogenes immunoserum rabbit, streptococcus...
lactococcus lactis immunoserum rabbit (UNII: AST1Y049U1) (lactococcus lactis immunoserum rabbit - UNII:AST1Y049U1) lactococcus ... PLEO POLY OM- lactococcus lactis immunoserum rabbit, streptococcus pyogenes immunoserum rabbit, streptococcus viridans group ... PLEO POLY OM- lactococcus lactis immunoserum rabbit, streptococcus pyogenes immunoserum rabbit, streptococcus viridans group ... PLEO POLY OM- lactococcus lactis immunoserum rabbit, streptococcus pyogenes immunoserum rabbit, streptococcus viridans group ...
https://dailymed.nlm.nih.gov/dailymed/drugInfo.cfm?setid=68835302-9345-413f-b843-88ec4541e9aa&audience=consumer
Heterologous production of bisin by Lactococcus lactis - Experts@MinnesotaHeterologous production of bisin by Lactococcus lactis - [email protected]
Powered by Pure, Scopus & Elsevier Fingerprint Engine™ © 2022 Elsevier B.V We use cookies to help provide and enhance our service and tailor content. By continuing you agree to the use of cookies. Log in to Pure. ...
https://experts.umn.edu/en/projects/heterologous-production-of-bisin-by-lactococcus-lactis-4
Minimal generation time on milk or M17 medium - Bacteria Lactococcus lactis - BNID 100464Minimal generation time on milk or M17 medium - Bacteria Lactococcus lactis - BNID 100464
Lactococcus lactis subsp. cremoris MG1614 (Gasson 1983) and its derivatives were grown at 30°C in M17 medium (Oxoid, UK) or in ... Bacteria Lactococcus lactis. Reference. Holubová J, Josephsen J. Potential of AbiS as defence mechanism determined by ...
https://bionumbers.hms.harvard.edu/bionumber.aspx?s=n&v=6&id=100464
Structure-guided engineering of Lactococcus lactis alcohol dehydrogenase LlAdhA for improved conversion of isobutyraldehyde to...Structure-guided engineering of Lactococcus lactis alcohol dehydrogenase LlAdhA for improved conversion of isobutyraldehyde to...
We have determined the X-ray crystal structures of the NADH-dependent alcohol dehydrogenase LlAdhA from Lactococcus lactis and ... Structure-guided engineering of Lactococcus lactis alcohol dehydrogenase LlAdhA for improved conversion of isobutyraldehyde to ... Structure-guided engineering of Lactococcus lactis alcohol dehydrogenase LlAdhA for improved conversion of isobutyraldehyde to ... Structure-guided engineering of Lactococcus lactis alcohol dehydrogenase LlAdhA for improved conversion of isobutyraldehyde to ...
https://authors.library.caltech.edu/38883/
Neutralizing-antibody-mediated protection of chickens against infectious bursal disease via one-time vaccination with...Neutralizing-antibody-mediated protection of chickens against infectious bursal disease via one-time vaccination with...
Lactococcus lactis NZ3900 strain and its matching plasmid pNZ8149 could express the recombinant fusion protein VP2-RCK in a ... The protective efficacy of r-L. lactis-OptiVP2-RCK (100%) was better than r-L. lactis-OptiVP2 (0%) which prove RCK protein ... fusion protein VP2-RCK was expressed in a soluble and stable form in the cytoplasm of the recombinant Lactococcus lactis. ... Here, we construct a recombinant Lactococcus co-expressing the major IBDV antigens VP2 and RCK protein of Salmonella enterica ...
https://microbialcellfactories.biomedcentral.com/articles/10.1186/s12934-019-1061-9
Presence of galactose in precultures induces lacS and leads to short lag phase in lactose-grown Lactococcus lactis culturesPresence of galactose in precultures induces lacS and leads to short lag phase in lactose-grown Lactococcus lactis cultures
We observed that Lactococcus lactis using the high-affinity lactose-phosphotransferase system excreted galactose towards the ... We observed that Lactococcus lactis using the high-affinity lactose-phosphotransferase system excreted galactose towards the ... Presence of galactose in precultures induces lacS and leads to short lag phase in lactose-grown Lactococcus lactis cultures ... Characterization of metabolism and robustness of Lactococcuccus lactis under different fermentation conditions for production ...
https://research.chalmers.se/en/publication/6d5b0868-c719-4474-a2e9-555bca220eca
Lactococcus lactis
Is the glycolytic flux in Lactococcus lactis primarily controlled by the redox charge? Kinetics of NAD(+) and NADH pools ... Time dependent responses of glycolytic intermediates in a detailed glycolytic model of Lactococcus lactis during glucose run- ... The involvement of nicotinamide adenine nucleotides (NAD(+), NADH) in the regulation of glycolysis in Lactococcus lactis was ... We interpret the data as showing that the glycolytic flux in wild type L. lactis is not primarily controlled at the level of ...
https://demo.fairdomhub.org/organisms/1348724285
Intramammary infusion of a live culture of Lactococcus lactis in ewes to treat staphylococcal mastitis<...
Intramammary infusion of a live culture of Lactococcus lactis in ewes to treat staphylococcal mastitis. In: Journal of Medical ... We present an evaluation, in two trials, of the efficacy of an intramammary infusion of a live culture of Lactococcus lactis ... We present an evaluation, in two trials, of the efficacy of an intramammary infusion of a live culture of Lactococcus lactis ... We present an evaluation, in two trials, of the efficacy of an intramammary infusion of a live culture of Lactococcus lactis ...
https://moh-it.pure.elsevier.com/en/publications/intramammary-infusion-of-a-live-culture-of-lactococcus-lactis-in-
Lactococcus lactis ssp. lactis
... is unique because it can grow both aerobically and anaerobically (both in the presence or ... Because of this, L. lactis ssp. lactis can continue to work in volatile conditions. ... In silage inoculants, L. lactis significantly reduces the growth of yeasts and molds and improves dry matter recover in the top ...
https://zh.microsynergies.com/microbial-cultures/Lactococcus-lactis-ssp.-lactis
MeSH | Lactococcus lactis (D013294)MeSH | Lactococcus lactis (D013294)
Organisms [B] » Bacteria [B03] » Gram-Positive Bacteria » Lactobacillales » Streptococcaceae » Lactococcus » Lactococcus lactis ... Organisms [B] » Bacteria [B03] » Gram-Positive Bacteria » Gram-Positive Cocci » Streptococcaceae » Lactococcus » Lactococcus ... A non-pathogenic species of LACTOCOCCUS found in DAIRY PRODUCTS and responsible for the souring of MILK and the production of ...
https://www.cdek.liu.edu/mesh/D013294/
Nutrients | Free Full-Text | Lactose Maldigestion, Malabsorption, and Intolerance: A Comprehensive Review with a Focus on...Nutrients | Free Full-Text | Lactose Maldigestion, Malabsorption, and Intolerance: A Comprehensive Review with a Focus on...
... composed of Bifidobacterium lactis W51, Lactobacillus acidophilus W22, Lactobacillus plantarum W21, Lactococcus lactis W19, and ... Li, J.; Zhang, W.; Wang, C.; Yu, Q.; Dai, R.; Pei, X. Lactococcus lactis expressing food-grade β-galactosidase alleviates ...
https://www.mdpi.com/2072-6643/10/11/1599/htm
Functional and Morphological Adaptation to Peptidoglycan Precursor Alteration in <em>Lactococcus lactis</em> - ePrints -...Functional and Morphological Adaptation to Peptidoglycan Precursor Alteration in <em>Lactococcus lactis</em> - ePrints -...
Functional and Morphological Adaptation to Peptidoglycan Precursor Alteration in Lactococcus lactis. Lookup NU author(s): Dr ... A set of L. lactis mutant strains in which the normal D-Ala-ended precursors were partially or totally replaced by D-Lac-ended ... Peptidoglycan substrate alteration was investigated in the Gram-positive bacterium Lactococcus lactis by substituting the ... of this altered pathway was necessary to increase the level of vancomycin resistance conferred by the utilization of D-Lac- ...
https://eprints.ncl.ac.uk/157650
IMSEAR at SEARO: Risk Assessment of Lactococcus lactis W58 Used as "Other Substances"
Risk Assessment of Lactococcus lactis W58 Used as Other Substances. Authors: Yazdankhah, Siamak. Grahek-Ogden, Danica. Eckner ... Risk Assessment of Lactococcus lactis W58 Used as Other Substances. European Journal of Nutrition and Food Safety. 2018 Oct; ... The present report is a risk assessment of Lactococcus lactis W58, and it is based on previous risk assessments and articles ... Other sources of L. lactis W58, such as foods, have not been included in the present risk assessment. VKM concludes that it is ...
https://imsear.searo.who.int/handle/123456789/189573
Aortic valve replacement due to lactococcus lactis infective endocarditis | EJCMAortic valve replacement due to lactococcus lactis infective endocarditis | EJCM
Four blood cultures were taken from patient and lactococcus lactis was seen in one of them. 1,5*2,1 cm sized vegetations on the ... Lactococcus lactis is a mesophilic and microaerophilic fermenting bacteria, used for fermented food products production. It can ... C.Rostagno,P.Pecile,P.L.Stefano.Early Lactococcus lactis endocarditis after mitral valve repair:a case report and literature ... In this study we presented a 34 year old male patient underwent surgery, diagnosed with lactococcus lactis infective ...
https://www.ejcvsmed.org/articles/case-report/aortic-valve-replacement-due-lactococcus-lactis-infective-endocarditis/
Production of fermented yogurt by using of Lactococcus lactis ssp. - AnandpubProduction of fermented yogurt by using of Lactococcus lactis ssp. - Anandpub
Title: Production of fermented yogurt by using of Lactococcus lactis ssp. cremoris and fortified with jujube (Ziziphus jujuba) ... Production of fermented yogurt by using of Lactococcus lactis ssp. cremoris and fortified with jujube (Ziziphus jujuba) syrup. ... Production of fermented yogurt by using of Lactococcus lactis ssp. cremoris and fortified with jujube (Ziziphus jujuba) syrup. ... W/W basis incorporated in the buffalo milk fermented with Lactococcus lactis ssp. cremoris and then fermented buffalo milk ...
https://www.anandpub.com/tag/production-of-fermented-yogurt-by-using-of-lactococcus-lactis-ssp/
Site information for: CcpA in species Lactococcus lactis; genome accession: NC 009004.1Site information for: CcpA in species Lactococcus lactis; genome accession: NC 009004.1
Lactococcus lactis - NC_009004.1 TF. CcpA [UniProtKB:Q9CF33, view regulon] Supporting Evidence. Binding site. Location. ...
http://collectf.org/EXPSITE_000244b0
Chemokine-Based ImmunotherapyChemokine-Based Immunotherapy
Lactococcus lactis (CXCL9-CXCL10). -. Production of a biologically active genetic fusion of CXCL9-CXCL10 by L. lactis [62]. ... L. lactis expressing (CXCL9-CXCL10). -. Production of a biologically active genetic fusion of CXCL9-CXCL10 by L. lactis [62]. ... Lactococcus lactis expressing CXCL10. -. Expression and secretion of mature biologically active CXCL10. [58,61]. ... L. lactis expressing XCL1. -. Production of feasible well-tolerated vector to deliver biological active XCL1 at a mucosal level ...
https://www.medscape.com/viewarticle/772651
Water Spotlight - Emerging Infectious Diseases journal - CDC
Lactococcus lactis subsp. lactis Infection in Waterfowl: First Confirmation in Animals Joaquín Goyache et al. Volume 7, Number ...
https://wwwnc.cdc.gov/eid/spotlight/water
Recombinant Lactococcus lactis subsp. cremoris Energy-coupling factor transporter ATP-binding protein EcfA1(ecfA1) - CusabioRecombinant Lactococcus lactis subsp. cremoris Energy-coupling factor transporter ATP-binding protein EcfA1(ecfA1) - Cusabio
Purchase Recombinant Lactococcus lactis subsp. cremoris Energy-coupling factor transporter ATP-binding protein EcfA1(ecfA1). It ... Recombinant Lactococcus lactis subsp. cremoris Energy-coupling factor transporter ATP-binding protein EcfA1(ecfA1). ... Recombinant Lactococcus lactis subsp. cremoris Energy-coupling factor transporter ATP-binding protein EcfA1(ecfA1) ...
https://www.cusabio.com/Recombinant-Protein/Recombinant-Lactococcus-lactis-subsp-cremoris-Energy-coupling-factor-transporter-ATP-binding-protein-EcfA1-ecfA1--376903.html
Zinc in PDB 6fi9: Crystal Structure of A Zinc-Responsive Marr Family Member, Lactococcus Lactis Zitr
Lactococcus Lactis Zitr, PDB code: 6fi9:. Jump to Zinc binding site number: 1; 2; 3; 4; 5; 6; 7; 8; 9; 10; ... Zinc binding site 1 out of 16 in the Crystal Structure of A Zinc-Responsive Marr Family Member, Lactococcus Lactis Zitr. Mono ... Zinc binding site 2 out of 16 in the Crystal Structure of A Zinc-Responsive Marr Family Member, Lactococcus Lactis Zitr. Mono ... Zinc binding site 3 out of 16 in the Crystal Structure of A Zinc-Responsive Marr Family Member, Lactococcus Lactis Zitr. Mono ...
http://zinc.atomistry.com/pdb6fi9.html
Lactose and β-Glucosides Metabolism and Its Regulation in Lactococcus lactis: A Review. - IBB PAS RepositoryLactose and β-Glucosides Metabolism and Its Regulation in Lactococcus lactis: A Review. - IBB PAS Repository
Aleksandrzak-Piekarczyk, Tamara (2013) Lactose and β-Glucosides Metabolism and Its Regulation in Lactococcus lactis: A Review. ... Lactose and β-Glucosides Metabolism and Its Regulation in Lactococcus lactis: A Review. ...
http://eprints.ibb.waw.pl/594/
Biogenic amine production by Lactococcus lactis subsp. cremoris strains in the model system of Dutch-type cheeseBiogenic amine production by Lactococcus lactis subsp. cremoris strains in the model system of Dutch-type cheese
The aim of this study was to compare the biogenic amine production of two starter strains of Lactococcus lactis subsp. cremoris ... Biogenic amine production by Lactococcus lactis subsp. cremoris strains in the model system of Dutch-type cheese. Food ... Biogenic amine production by Lactococcus lactis subsp. cremoris strains in the model system of Dutch-type cheese. DSpace ... Biogenic amine production by Lactococcus lactis subsp. cremoris strains in the model system of Dutch-type cheese. ...
https://publikace.k.utb.cz/handle/10563/1005285
Evaluation of Technological Attributes of Lactococcus lactis Cultures for Preparation of Dahi and Misti Dahi (Sweetened Dahi)Evaluation of Technological Attributes of Lactococcus lactis Cultures for Preparation of Dahi and Misti Dahi (Sweetened Dahi)
Alomar, J., Loubire, P., Delbes, C., Nouailee, S. and Montel, M.C. (2008). Effect of Lactococcus garivieae, Lactococcus lactis ... Methods: Six Lactococcus lactis were collected and tested for acidification profile as well as growth rate was also determined ... Acidification activity Antimicrobial activity Growth rate Lactococcus lactis Sensory evaluation. References. * ... 2003). Genetics of Proteolysis in Lactococcus lactis. In: Genetics of Lactic acid Bacteria. [B.J.B. Wood and W.M. de Vos (ed ...
https://arccjournals.com/journal/asian-journal-of-dairy-and-food-research/DR-1632
Lectin Affinity Chromatography | Semantic ScholarLectin Affinity Chromatography | Semantic Scholar
Lectin-Mediated Binding of Engineered Lactococcus lactis to Cancer Cells. *T. V. Plavec, Abida Zahirović, Petra Zadravec, J. ... are employed for surface display on the lactic acid bacterium Lactococcus lactis to see if they might serve as a carrier in ...
https://www.semanticscholar.org/paper/Lectin-Affinity-Chromatography-Freeze/a6b9de6ac88a88a76e103102aef32c647e05bc35
Ripening to convert ferment milk sugars to lactic acid : ButterRipening to convert ferment milk sugars to lactic acid : Butter
Lactococcus lactis subsp. lactis *Lactococcus lactis subsp. cremoris *Lactococcus lactis subsp. lactis biovar. diacetylactis * ... called Lactococcus), and Leuconostoc (at right). The culture commonly used mixture for butter is:. * ...
https://www.webexhibits.org/butter/culturing.html
Biomolecules | June 2015 - Browse ArticlesBiomolecules | June 2015 - Browse Articles
curvatus Beef3; Pediococcus acidilactici Bac3; Lactococcus lactis FLS1; Enterococcus faecium FS56-1; and Enterococcus ... faecium,/italic, FS56-1, ,italic,Pediococcus acidilactici,/italic, Bac3, ,italic,Lactococcus lactis,/italic, FS91-1, ,italic,En ...
https://www.mdpi.com/2218-273X/5/2
Optimasi Konsentrasi Nisin dan Lama Inkubasi untuk Induksi Ekspresi Protein Spike HCR pada Karier Lactococcus lactis -...Optimasi Konsentrasi Nisin dan Lama Inkubasi untuk Induksi Ekspresi Protein Spike HCR pada Karier Lactococcus lactis -...
Optimasi Konsentrasi Nisin dan Lama Inkubasi untuk Induksi Ekspresi Protein Spike HCR pada Karier Lactococcus lactis ... Optimasi Konsentrasi Nisin dan Lama Inkubasi untuk Induksi Ekspresi Protein Spike HCR pada Karier Lactococcus lactis. Sarjana ... Bakteri L. lactis yang telah dimodifikasi secara genetik untuk membawa protein HCR dari SARS-CoV-2 menjadi kandidat vaksin ... COVID-19, vaksin mukosal, nisin, L. lactis, live bacterial vector. Subjects:. 600 Technology (Applied sciences) , 615 ...
http://repository.ub.ac.id/id/eprint/194329/
Complete genome sequence of Lactococcus lactis IO-1, a lactic acid bacterium that utilizes xylose and produces high levels of l...
Complete genome sequence of Lactococcus lactis IO-1, a lactic acid bacterium that utilizes xylose and produces high levels of l ... Dive into the research topics of Complete genome sequence of Lactococcus lactis IO-1, a lactic acid bacterium that utilizes ...
https://waseda.pure.elsevier.com/en/publications/complete-genome-sequence-of-lactococcus-lactis-io-1-a-lactic-acid/fingerprints/
SubspLactobacillusStreptococcusCremorisStrainsMG1363BacteriumNisinEnterococcusStrainBacteriaFermentationGenomeSpeciesBiovarMicroorganismsRecombinantSilageProteinMetabolismHeterologousStaphylococcusCheeseRegulationYogurtAffinityEfficacyGrowthProductionCulturesMicrobeHumansStableSurfaceSearchCulturePresenceAssessmentLiveFoundPresent
Subsp8
  • Lactococcus lactis subsp. (harvard.edu)
  • Biogenic amine production by Lactococcus lactis subsp. (utb.cz)
  • was first identified in L. lactis subsp. (dairy-journal.org)
  • Nous avons d'abord identifié dans la souche IL1403 de L. lactis subsp. (dairy-journal.org)
  • To test if a single vector, nisin-controlled expression (NICE) system could be used to regulate expression of the pediocin operon in Streptococcus thermophilus, Lactococcus lactis subsp. (usda.gov)
  • thermophilus ST128, L. lactis subsp. (usda.gov)
  • Under carbohydrate starvation stress, we investigated the generation of non-culturable forms of three bacteriocin (nisin) producing Lactococcus lactis subsp. (2pressrelease.net)
  • Q9CDF7 2.A.7.5.3 Glucose:H+ symporter, GlcU (YxfA) (high specificity, low affinity) Lactococcus lactis subsp. (lbl.gov)
Lactobacillus7
  • Peptidoglycan substrate alteration was investigated in the Gram-positive bacterium Lactococcus lactis by substituting the peptidoglycan precursor biosynthesis genes of this bacterium for those of the vancomycin-resistant bacterium Lactobacillus plantarum. (ncl.ac.uk)
  • In this study, we developed a way to introduce a bacteriocin, which inhibits the growth of Listeria, into dairy LAB such as Streptococcus thermophilus, Lactococcus lactis and Lactobacillus casei. (usda.gov)
  • lactis and Lactobacillus casei, the intact pediocin operon was cloned into pMSP3535 immediately down stream of the nisA promoter (PnisA). (usda.gov)
  • Jarro-Dophilus® EPS helps support the health of the entire intestinal tract with a mixture of eight probiotic species from four different genera: Lactobacillus, Bifidobacterium, Lactococcus, and Pediococcus. (herbspro.com)
  • This protection is provided by a human protein, Elafin, which is artificially introduced into dairy produce bacteria ( Lactococcus lactis and Lactobacillus casei) . (aviesan.fr)
  • To this end, the human Elafin gene, isolated in collaboration with a team from the Institut Pasteau, was introduced in Lactococcus lactis and Lactobacillus casei, two food-grade bacteria found in dairy products. (aviesan.fr)
  • Simfort is a mix of 5 different probiotic strains Lactobacillus acidophillus, Lactobacillus casei, Lactococcus lactis, Bifidobacterium lactis and Bifidobacterium bifidum, which provide several health benefits of the intestine and helping to balance the intestinal microbiota. (vitaforeurope.com)
Streptococcus5
  • Acetoin production and citrate metabolism in Streptococcus lactis subspdiacetylactis. (arccjournals.com)
  • The bacteria are from the Bacilli type of Lactobacillales which includes Streptococcaceae (parent of Streptococcus and lactic Streptococcus , called Lactococcus ), and Leuconostoc (at right). (webexhibits.org)
  • In any case, it would be an interesting battle - the one between the Lactococcus and the Streptococcus. (smdp.com)
  • As contagens de bactérias láticas do queijo de coalho dos produtores A e B foram na ordem de 106 e as maiores contagens (109 UFC/g) foram observadas no queijo do produtor C. Foram selecionadas 49 bactérias láticas típicas das três propriedades e os gêneros predominantes foram: Enterococcus, Lactococcus, Streptococcus e Leuconostoc. (bvsalud.org)
  • The lactic acid bacteria counts of coalho cheese from producers A and B were 106 and the highest counts (109 UFC/g) were found in cheese samples from producer C. Forty-nine lactic acid bacteria from three rural properties were selected and predominant genera was Enterococcus, Lactococcus, Streptococcus and Leuconostoc. (bvsalud.org)
Cremoris1
  • Often, hard cheese is manufactured using the subspecies lactis and the subspecies cremoris is used to manufacture soft cheese. (micropia.nl)
Strains4
  • A set of L. lactis mutant strains in which the normal D-Ala-ended precursors were partially or totally replaced by D-Lac-ended precursors was generated. (ncl.ac.uk)
  • Optimization of this altered pathway was necessary to increase the level of vancomycin resistance conferred by the utilization of D-Lac-ended peptidoglycan precursors in the mutant strains. (ncl.ac.uk)
  • The AccuProbe- Enterococcus test is used to aid in the identification of atypical enterococci and to help differentiate between Enterococcus and Lactococcus strains. (cdc.gov)
  • lactis strains: MSU, 729, and F-116. (2pressrelease.net)
MG13631
  • The capacity of L. lactis MG1363 to regenerate NAD(+) was manipulated either by turning on NADH oxidase activity or by knocking out the gene encoding lactate dehydrogenase (LDH). (fairdomhub.org)
Bacterium2
  • Two lectins, B subunit of bacterial Shiga holotoxin and fungal Clitocybe nebularis lectin, are employed for surface display on the lactic acid bacterium Lactococcus lactis to see if they might serve as a carrier in targeted drug delivery when coupled to a therapeutic moiety. (semanticscholar.org)
  • She pursued a PhD on the subject of prophage-encoded bacteriophage resistance mechanisms in the dairy bacterium Lactococcus lactis . (ucc.ie)
Nisin2
  • Penelitian ini bertujuan untuk menentukan konsentrasi nisin dan lama inkubasi yang optimal untuk menginduksi ekspresi protein HCR pada karier bakteri rekombinan L. lactis NZ3900/pNZ8149-HCR. (ub.ac.id)
  • Penggunaan nisin untuk menginduksi ekspresi protein HCR secara optimal pada bakteri rekombinan L. lactis NZ3900/pNZ8149-HCR didapatkan dengan menggunakan konsentrasi 40 ng/ mL dan inkubasi selama 9 jam. (ub.ac.id)
Enterococcus1
  • Effect of Lactococcus garivieae , Lactococcus lactis and Enterococcus faecalis on the behavior of Staphylococcus aureus in microfiltered milk. (arccjournals.com)
Strain6
  • Lactococcus lactis NZ3900 strain and its matching plasmid pNZ8149 could express the recombinant fusion protein VP2-RCK in a soluble form in the cytoplasm. (biomedcentral.com)
  • We constructed a Lactococcus lactis mutant strain in which recombinant or heterologous exported proteins are stable. (dairy-journal.org)
  • lactis strain IL1403. (dairy-journal.org)
  • In the future, the mutant htrA strain should constitute an efficient tool to improve yields of heterologous exported proteins in L. lactis . (dairy-journal.org)
  • From the phylogenetic analysis based on 16S rDNA sequence, the strain W-44 was identified as Lactococcus lactis. (kribb.re.kr)
  • They modified a strain of Lactococcus lactis , which is safe for humans to eat and is often used in cheese production, to deliver an enzyme that can break down beta-lactam antibiotics. (medscape.com)
Bacteria3
  • Lactococcus lactis is a mesophilic and microaerophilic fermenting bacteria, used for fermented food products production. (ejcvsmed.org)
  • A lysate produced by fermentation of probiotic bacteria Lactococcus lactis . (zelens.com)
  • Of 20 bacteria isolated, 19 showed inhibition halos on the three pathogenic bacteria with diameter of 2 to 15 mm, and the largest halos were formed by Lactococcus lactis ssp lactis on S. enterica and S. aureus. (bvsalud.org)
Fermentation1
  • After the fermentation of citrus fruit with L. lactis W-44, the contents of naringenin and besperetin, bioactive flavonoid aglycones, were increased about ten-fold and six-fold, respectively. (kribb.re.kr)
Genome1
  • is the sole extra-cellular housekeeping protease in L. lactis , in agreement with the analysis of the complete IL1403 genome sequence. (dairy-journal.org)
Species4
  • Assessment of the feed additive consisting of Lactococcus lactis NCIMB 30117 for all animal species for the renewal of its authorisation (Chr. (europa.eu)
  • Following a request from the European Commission, EFSA was asked to deliver a scientific opinion on the assessment of the application for renewal of Lactococcus lactis NCIMB 30117 as a technological additive for use in forage for all animal species. (europa.eu)
  • A non-pathogenic species of LACTOCOCCUS found in DAIRY PRODUCTS and responsible for the souring of MILK and the production of LACTIC ACID . (liu.edu)
  • Although common species causing infective endocarditis include streptococci, staphylococci, enterococci and fastidious gram negative coccobacilli, aortic valve replacement due to lactococcus lactis infective endocarditis seen in the literature, even if rarely. (ejcvsmed.org)
Biovar1
Microorganisms1
  • We offer a huge selection of easy-to-handle tools for efficient gene expression in different microorganisms like Bacillus megaterium , Bacillus subtilis, Lactococcus lactis, Escherichia coli , and yeast. (mobitec.com)
Recombinant2
  • Here, we construct a recombinant Lactococcus co-expressing the major IBDV antigens VP2 and RCK protein of Salmonella enterica to prevent IBD. (biomedcentral.com)
  • The recombinant fusion protein VP2-RCK was expressed in a soluble and stable form in the cytoplasm of the recombinant Lactococcus lactis . (biomedcentral.com)
Silage1
  • In silage inoculants, L. lactis significantly reduces the growth of yeasts and molds and improves dry matter recover in the top layer of forage. (microsynergies.com)
Protein2
  • The protective efficacy of r- L. lactis -OptiVP2-RCK (100%) was better than r- L. lactis -OptiVP2 (0%) which prove RCK protein played its unique role. (biomedcentral.com)
  • Bakteri L. lactis yang telah dimodifikasi secara genetik untuk membawa protein HCR dari SARS-CoV-2 menjadi kandidat vaksin mukosal untuk COVID-19. (ub.ac.id)
Metabolism1
  • Lactose and β-Glucosides Metabolism and Its Regulation in Lactococcus lactis: A Review. (waw.pl)
Heterologous1
  • Optimising the production of heterologous exported proteins in Lactococcus lactis by inactivation of HtrA, the unique housekeeping surface protease. (dairy-journal.org)
Staphylococcus1
  • queijo, Lactococcus, Staphylococcus aureus. (bvsalud.org)
Cheese2
  • On a daily basis, L. lactis is used for manufacturing dairy products, like buttermilk and cheese. (micropia.nl)
  • L. lactis is also used for the manufacture of both hard and soft cheese. (micropia.nl)
Regulation1
  • The involvement of nicotinamide adenine nucleotides (NAD(+), NADH) in the regulation of glycolysis in Lactococcus lactis was investigated by using (13)C and (31)P NMR to monitor in vivo the kinetics … of the pools of NAD(+), NADH, ATP, inorganic phosphate (P(i)), glycolytic intermediates, and end products derived from a pulse of glucose. (fairdomhub.org)
Yogurt1
  • Production of fermented yogurt by using of Lactococcus lactis ssp. (anandpub.com)
Affinity1
  • We observed that Lactococcus lactis using the high-affinity lactose-phosphotransferase system excreted galactose towards the end of the lactose consumption phase. (chalmers.se)
Efficacy1
  • We present an evaluation, in two trials, of the efficacy of an intramammary infusion of a live culture of Lactococcus lactis for the treatment of subclinical and clinical mastitis in ewes. (elsevier.com)
Growth1
  • Six Lactococcus lactis were collected and tested for acidification profile as well as growth rate was also determined in milk with (15%) and without sucrose. (arccjournals.com)
Production1
  • à l'avenir, la souche mutante htrA de L. lactis devrait donc constituer un outil performant pour optimiser les rendements de production de protéines hétérologues exportées. (dairy-journal.org)
Cultures3
  • Four blood cultures were taken from patient and lactococcus lactis was seen in one of them. (ejcvsmed.org)
  • The present study was aimed to evaluate the technological attributes of selected six Lactococcus lactis cultures namely NCDC 193, 125, 128, 94, 97 and 314 for preparation of Dahi and Misti dahi . (arccjournals.com)
  • Among six cultures three Lactococcus lactis cultures NCDC 314, 94 and 97 have shown zone of inhibition against all four test organisms. (arccjournals.com)
Microbe2
  • The microbe is called the Lactococcus Lactis. (smdp.com)
  • When a herbivore, for example a cow, eats grass with L. lactis on its surface, the cow ingests the microbe. (micropia.nl)
Humans1
  • Lactococcus lactis is an uncommon cause of invasive disease in humans. (austin.org.au)
Stable1
  • Chez Lactococcus lactis , nous avons construit une souche mutante qui permet d'exporter des protéines recombinantes ou hétérologues sous une forme stable. (dairy-journal.org)
Surface1
  • L'inactivation de son gène a ensuite montré qu'elle assure, à la surface cellulaire, une fonction de ménage en éliminant des protéines anormales et/ou mal repliées, et qu'elle est aussi responsable de la maturation de protéines exportées naturelles, comme la bactériolysine AcmA de L. lactis . (dairy-journal.org)
Search1
  • The present report is a risk assessment of Lactococcus lactis W58, and it is based on previous risk assessments and articles retrieved from a literature search. (who.int)
Culture1
  • We present a case of L. lactis liver abscess in an immunocompetent adult, apparently related to consumption of live culture yoghurt. (austin.org.au)
Presence1
  • Samples were then analyzed for the presence of two Lactococcus lactis phage groups (936 and c2) and quantification was done by qPCR. (cdc.gov)
Assessment1
  • Other sources of L. lactis W58, such as foods, have not been included in the present risk assessment. (who.int)
Live1
  • De milliarder af bakterier i kapslerne, det løse pulver eller poserne er blevet sat i en slags "dvaletilstand" under produktionen, så de kan opbevares i længere tid, og de har brug for væske for at komme til live igen. (nutritioncompany.eu)
Found1
  • L. lactis can be found in a dormant state on plants. (micropia.nl)
Present1
  • When no oxygen is present, L. lactis produces lactic acid with the help of an enzyme, called lactate-dehydrogenase. (micropia.nl)
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Omni Biotic 6 | Symptome, Ursachen von Krankheiten (symptome.ch)
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Antifungal Activity of Bacteriocins of Lactic Acid Bacteria from Some Nigerian Fermented Foods (scialert.net)
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Ver Autor MARIA ELENA FATIMA NADER
Ver Autor MARIA ELENA FATIMA NADER (ri.conicet.gov.ar)
Publicações
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