A non-pathogenic species of LACTOCOCCUS found in DAIRY PRODUCTS and responsible for the souring of MILK and the production of LACTIC ACID.
A genus of gram-positive, coccoid bacteria mainly isolated from milk and milk products. These bacteria are also found in plants and nonsterile frozen and dry foods. Previously thought to be a member of the genus STREPTOCOCCUS (group N), it is now recognized as a separate genus.
A 34-amino acid polypeptide antibiotic produced by Streptococcus lactis. It has been used as a food preservative in canned fruits and vegetables, and cheese.
A nutritious food consisting primarily of the curd or the semisolid substance formed when milk coagulates.
Proteins found in any species of bacterium.
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
Viruses whose hosts are bacterial cells.
Substances elaborated by specific strains of bacteria that are lethal against other strains of the same or related species. They are protein or lipopolysaccharide-protein complexes used in taxonomy studies of bacteria.
The functional hereditary units of BACTERIA.
A family of BACTERIOPHAGES and ARCHAEAL VIRUSES which are characterized by long, non-contractile tails.
Any of the processes by which cytoplasmic or intercellular factors influence the differential control of gene action in bacteria.
A genus of gram-positive, facultatively anaerobic bacteria whose growth is dependent on the presence of a fermentable carbohydrate. It is nonpathogenic to plants and animals, including humans.
A genus of gram-positive, microaerophilic, rod-shaped bacteria occurring widely in nature. Its species are also part of the many normal flora of the mouth, intestinal tract, and vagina of many mammals, including humans. Pathogenicity from this genus is rare.
The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.
Deoxyribonucleic acid that makes up the genetic material of bacteria.
Anaerobic degradation of GLUCOSE or other organic nutrients to gain energy in the form of ATP. End products vary depending on organisms, substrates, and enzymatic pathways. Common fermentation products include ETHANOL and LACTIC ACID.
Extrachromosomal, usually CIRCULAR DNA molecules that are self-replicating and transferable from one organism to another. They are found in a variety of bacterial, archaeal, fungal, algal, and plant species. They are used in GENETIC ENGINEERING as CLONING VECTORS.
The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.
An ascomycetous yeast of the fungal family Saccharomycetaceae, order SACCHAROMYCETALES.
The insertion of recombinant DNA molecules from prokaryotic and/or eukaryotic sources into a replicating vehicle, such as a plasmid or virus vector, and the introduction of the resultant hybrid molecules into recipient cells without altering the viability of those cells.
The white liquid secreted by the mammary glands. It contains proteins, sugar, lipids, vitamins, and minerals.
In bacteria, a group of metabolically related genes, with a common promoter, whose transcription into a single polycistronic MESSENGER RNA is under the control of an OPERATOR REGION.
A multistage process that includes cloning, physical mapping, subcloning, determination of the DNA SEQUENCE, and information analysis.
A disaccharide of GLUCOSE and GALACTOSE in human and cow milk. It is used in pharmacy for tablets, in medicine as a nutrient, and in industry.
Milk modified with controlled FERMENTATION. This should not be confused with KAFFIR LIME or with KAFFIR CORN.
A product of fermentation. It is a component of the butanediol cycle in microorganisms. In mammals it is oxidized to carbon dioxide.
A parasexual process in BACTERIA; ALGAE; FUNGI; and ciliate EUKARYOTA for achieving exchange of chromosome material during fusion of two cells. In bacteria, this is a uni-directional transfer of genetic material; in protozoa it is a bi-directional exchange. In algae and fungi, it is a form of sexual reproduction, with the union of male and female gametes.
Carrier of aroma of butter, vinegar, coffee, and other foods.
A sequence of successive nucleotide triplets that are read as CODONS specifying AMINO ACIDS and begin with an INITIATOR CODON and end with a stop codon (CODON, TERMINATOR).
A species of thermophilic, gram-positive bacteria found in MILK and milk products.
The presence of bacteria, viruses, and fungi in food and food products. This term is not restricted to pathogenic organisms: the presence of various non-pathogenic bacteria and fungi in cheeses and wines, for example, is included in this concept.
The industry concerned with processing, preparing, preserving, distributing, and serving of foods and beverages.
Viruses whose host is Streptococcus.
Directed modification of the gene complement of a living organism by such techniques as altering the DNA, substituting genetic material by means of a virus, transplanting whole nuclei, transplanting cell hybrids, etc.
Any liquid or solid preparation made specifically for the growth, storage, or transport of microorganisms or other types of cells. The variety of media that exist allow for the culturing of specific microorganisms and cell types, such as differential media, selective media, test media, and defined media. Solid media consist of liquid media that have been solidified with an agent such as AGAR or GELATIN.
A genus of gram-positive, facultatively anaerobic bacteria whose growth is dependent on the presence of a fermentable carbohydrate. No endospores are produced. Its organisms are found in fermenting plant products and are nonpathogenic to plants and animals, including humans.
Structures within the nucleus of bacterial cells consisting of or containing DNA, which carry genetic information essential to the cell.
Raw and processed or manufactured milk and milk-derived products. These are usually from cows (bovine) but are also from goats, sheep, reindeer, and water buffalo.
Allosteric enzymes that regulate glycolysis and gluconeogenesis. These enzymes catalyze phosphorylation of fructose-6-phosphate to either fructose-1,6-bisphosphate (PHOSPHOFRUCTOKINASE-1 reaction), or to fructose-2,6-bisphosphate (PHOSPHOFRUCTOKINASE-2 reaction).
Use of restriction endonucleases to analyze and generate a physical map of genomes, genes, or other segments of DNA.
A species of gram-negative, facultatively anaerobic, rod-shaped bacteria (GRAM-NEGATIVE FACULTATIVELY ANAEROBIC RODS) commonly found in the lower part of the intestine of warm-blooded animals. It is usually nonpathogenic, but some strains are known to produce DIARRHEA and pyogenic infections. Pathogenic strains (virotypes) are classified by their specific pathogenic mechanisms such as toxins (ENTEROTOXIGENIC ESCHERICHIA COLI), etc.
The degree of similarity between sequences of amino acids. This information is useful for the analyzing genetic relatedness of proteins and species.
The study, utilization, and manipulation of those microorganisms capable of economically producing desirable substances or changes in substances, and the control of undesirable microorganisms.
Salts and esters of CHOLIC ACID.
Rupture of bacterial cells due to mechanical force, chemical action, or the lytic growth of BACTERIOPHAGES.
A subclass of EXOPEPTIDASES that act on the free N terminus end of a polypeptide liberating a single amino acid residue. EC 3.4.11.
A species of rod-shaped, LACTIC ACID bacteria used in PROBIOTICS and SILAGE production.
A mixture of related phosphoproteins occurring in milk and cheese. The group is characterized as one of the most nutritive milk proteins, containing all of the common amino acids and rich in the essential ones.
Discrete segments of DNA which can excise and reintegrate to another site in the genome. Most are inactive, i.e., have not been found to exist outside the integrated state. DNA transposable elements include bacterial IS (insertion sequence) elements, Tn elements, the maize controlling elements Ac and Ds, Drosophila P, gypsy, and pogo elements, the human Tigger elements and the Tc and mariner elements which are found throughout the animal kingdom.
The heritable modification of the properties of a competent bacterium by naked DNA from another source. The uptake of naked DNA is a naturally occuring phenomenon in some bacteria. It is often used as a GENE TRANSFER TECHNIQUE.
A normal intermediate in the fermentation (oxidation, metabolism) of sugar. The concentrated form is used internally to prevent gastrointestinal fermentation. (From Stedman, 26th ed)
The outermost layer of a cell in most PLANTS; BACTERIA; FUNGI; and ALGAE. The cell wall is usually a rigid structure that lies external to the CELL MEMBRANE, and provides a protective barrier against physical or chemical agents.
The application of knowledge to the food industry.
A genus of gram-positive, coccoid bacteria whose organisms occur in pairs or chains. No endospores are produced. Many species exist as commensals or parasites on man or animals with some being highly pathogenic. A few species are saprophytes and occur in the natural environment.
An enzyme that catalyzes the conversion of alpha D-glucose 1-phosphate to alpha D-glucose 6-phosphate. EC 5.4.2.2.
A rod-shaped, gram-positive, non-acid-fast, non-spore-forming, non-motile bacterium that is a genus of the family Bifidobacteriaceae, order Bifidobacteriales, class ACTINOBACTERIA. It inhabits the intestines and feces of humans as well as the human vagina.
An enzyme that catalyzes the endonucleolytic cleavage to 3'-phosphomononucleotide and 3'-phospholigonucleotide end-products. It can cause hydrolysis of double- or single-stranded DNA or RNA. (From Enzyme Nomenclature, 1992) EC 3.1.31.1.
A tetrameric enzyme that, along with the coenzyme NAD+, catalyzes the interconversion of LACTATE and PYRUVATE. In vertebrates, genes for three different subunits (LDH-A, LDH-B and LDH-C) exist.
A family of gram-positive bacteria found regularly in the mouth and intestinal tract of man and other animals, in food and dairy products, and in fermenting vegetable juices. A few species are highly pathogenic.

Novel characteristic for distinguishing Lactococcus lactis subsp. lactis from subsp. cremoris. (1/1459)

Lactococcus lactis strains were examined for their ability to produce gamma-aminobutyric acid (GABA). Results showed that strains of L. lactis subsp. lactis were able to produce this acid, whereas L. lactis subsp. cremoris were not. GABA production thus represents another effective characteristic for distinguishing L. lactis subsp. lactis from L. lactis subsp. cremoris.  (+info)

Mechanism of citrate metabolism in Lactococcus lactis: resistance against lactate toxicity at low pH. (2/1459)

Measurement of the flux through the citrate fermentation pathway in resting cells of Lactococcus lactis CRL264 grown in a pH-controlled fermentor at different pH values showed that the pathway was constitutively expressed, but its activity was significantly enhanced at low pH. The flux through the citrate-degrading pathway correlated with the magnitude of the membrane potential and pH gradient that were generated when citrate was added to the cells. The citrate degradation rate and proton motive force were significantly higher when glucose was metabolized at the same time, a phenomenon that could be mimicked by the addition of lactate, the end product of glucose metabolism. The results clearly demonstrate that citrate metabolism in L. lactis is a secondary proton motive force-generating pathway. Although the proton motive force generated by citrate in cells grown at low pH was of the same magnitude as that generated by glucose fermentation, citrate metabolism did not affect the growth rate of L. lactis in rich media. However, inhibition of growth by lactate was relieved when citrate also was present in the growth medium. Citrate did not relieve the inhibition by other weak acids, suggesting a specific role of the citrate transporter CitP in the relief of inhibition. The mechanism of citrate metabolism presented here provides an explanation for the resistance to lactate toxicity. It is suggested that the citrate metabolic pathway is induced under the acidic conditions of the late exponential growth phase to make the cells (more) resistant to the inhibitory effects of the fermentation product, lactate, that accumulates under these conditions.  (+info)

A general method for selection of alpha-acetolactate decarboxylase-deficient Lactococcus lactis mutants to improve diacetyl formation. (3/1459)

The enzyme acetolactate decarboxylase (Ald) plays a key role in the regulation of the alpha-acetolactate pool in both pyruvate catabolism and the biosynthesis of the branched-chain amino acids, isoleucine, leucine, and valine (ILV). This dual role of Ald, due to allosteric activation by leucine, was used as a strategy for the isolation of Ald-deficient mutants of Lactococcus lactis subsp. lactis biovar diacetylactis. Such mutants can be selected as leucine-resistant mutants in ILV- or IV-prototrophic strains. Most dairy lactococcus strains are auxotrophic for the three amino acids. Therefore, the plasmid pMC004 containing the ilv genes (encoding the enzymes involved in the biosynthesis of IV) of L. lactis NCDO2118 was constructed. Introduction of pMC004 into ILV-auxotrophic dairy strains resulted in an isoleucine-prototrophic phenotype. By plating the strains on a chemically defined medium supplemented with leucine but not valine and isoleucine, spontaneous leucine-resistant mutants were obtained. These mutants were screened by Western blotting with Ald-specific antibodies for the presence of Ald. Selected mutants lacking Ald were subsequently cured of pMC004. Except for a defect in the expression of Ald, the resulting strain, MC010, was identical to the wild-type strain, as shown by Southern blotting and DNA fingerprinting. The mutation resulting in the lack of Ald in MC010 occurred spontaneously, and the strain does not contain foreign DNA; thus, it can be regarded as food grade. Nevertheless, its application in dairy products depends on the regulation of genetically modified organisms. These results establish a strategy to select spontaneous Ald-deficient mutants from transformable L. lactis strains.  (+info)

Characterization of the divergent sacBK and sacAR operons, involved in sucrose utilization by Lactococcus lactis. (4/1459)

The divergently transcribed sacBK and sacAR operons, which are involved in the utilization of sucrose by Lactococcus lactis NZ9800, were examined by transcriptional and gene inactivation studies. Northern analyses of RNA isolated from cells grown at the expense of different carbon sources revealed three sucrose-inducible transcripts: one of 3.2 kb containing sacB and sacK, a second of 3.4 kb containing sacA and sacR, and a third of 1.8 kb containing only sacR. The inactivation of the sacR gene by replacement recombination resulted in the constitutive transcription of the sacBK and sacAR operons in the presence of different carbon sources, indicating that SacR acts as a repressor of transcription.  (+info)

Membrane topology of the lactococcal bacteriocin ATP-binding cassette transporter protein LcnC. Involvement of LcnC in lactococcin a maturation. (5/1459)

Many non-lantibiotic bacteriocins of lactic acid bacteria are produced as precursors with N-terminal leader peptides different from those present in preproteins exported by the general sec-dependent (type II) secretion pathway. These bacteriocins utilize a dedicated (type I) secretion system for externalization. The secretion apparatus for the lactococcins A, B, and M/N (LcnA, B, and M/N) from Lactococcus lactis is composed of the two membrane proteins LcnC and LcnD. LcnC belongs to the ATP-binding cassette transporters, whereas LcnD is a protein with similarities to other accessory proteins of type I secretion systems. This paper shows that the N-terminal part of LcnC is involved in the processing of the precursor of LcnA. By making translational fusions of LcnC to the reporter proteins beta-galactosidase (LacZ) and alkaline phosphatase (PhoA*), it was shown that both the N- and C-terminal parts of LcnC are located in the cytoplasm. As the N terminus of LcnC is required for LcnA maturation and is localized in the cytoplasm, we conclude that the processing of the bacteriocin LcnA to its mature form takes place at the cytosolic side of the cytoplasmic membrane.  (+info)

Hydrolysis of alphas1- and beta-casein-derived peptides with a broad specificity aminopeptidase and proline specific aminopeptidases from Lactococcus lactis subsp. cremoris AM2. (6/1459)

Aminopeptidase hydrolysis of alpha(s)1 - and beta-casein-derived synthetic peptides containing non-consecutive and consecutive proline residues was characterised. Aminopeptidase P (Pep P) (EC 3.4.11.9) or post-proline dipeptidyl aminopeptidase (PPDA) (EC 3.4.14.5) along with lysine-paranitroanilide hydrolase (KpNA-H) (EC 3.4.11.1) activities are required in the degradation of peptides containing non-consecutive proline residues. However, both Pep P and PPDA along with KpNA-H are required for hydrolysis of peptides containing consecutive proline residues. The results demonstrate the mechanism by which combinations of purified general and proline specific aminopeptidases from Lactococcus lactis subsp. cremoris AM2 hydrolyse peptides containing proline residues.  (+info)

Regulation of expression of the Lactococcus lactis histidine operon. (7/1459)

In Lactococcus lactis, the his operon contains all the genes necessary for histidine biosynthesis. It is transcribed from a unique promoter, localized 300 bp upstream of the first gene. The region corresponding to the untranslated 5' end of the transcript, named the his leader region, displays the typical features of the T box transcriptional attenuation mechanism which is involved in the regulation of many amino acid biosynthetic operons and tRNA synthetase genes in gram-positive bacteria. Here we describe the regulation of transcription of the his operon by the level of histidine in the growth medium. In the absence of histidine, two transcripts are present. One covers the entire operon, while the other stops at a terminator situated about 250 bp downstream of the transcription start point. DNA sequences implicated in regulation of the his operon were identified by transcriptional fusion with luciferase genes and site-directed mutagenesis. In addition to the previously defined sequences necessary for effective T-box-mediated regulation, new essential regions were identified. Eighteen percent of the positions of the his leader region were found to differ in seven distantly related strains of L. lactis. Analysis of the variable positions supports the folding model of the central part of the his leader region. Lastly, in addition to the T-box-mediated regulation, the operon is regulated at the level of initiation of transcription, which is repressed in the presence of histidine. An operator site, necessary for full repression, overlaps the terminator involved in the T box attenuation mechanism. The functionality of the operator is altered on plasmids with low and high copy numbers, suggesting that supercoiling may play a role in the expression of the his operon. The extents of regulation at the levels of initiation and attenuation of transcription are 6- to 8-fold and 14-fold, respectively. Together, the two levels of control allow a 120-fold range of regulation of the L. lactis operon by histidine.  (+info)

Disruption and analysis of the clpB, clpC, and clpE genes in Lactococcus lactis: ClpE, a new Clp family in gram-positive bacteria. (8/1459)

In the genome of the gram-positive bacterium Lactococcus lactis MG1363, we have identified three genes (clpC, clpE, and clpB) which encode Clp proteins containing two conserved ATP binding domains. The proteins encoded by two of the genes belong to the previously described ClpB and ClpC families. The clpE gene, however, encodes a member of a new Clp protein family that is characterized by a short N-terminal domain including a putative zinc binding domain (-CX2CX22CX2C-). Expression of the 83-kDa ClpE protein as well as of the two proteins encoded by clpB was strongly induced by heat shock and, while clpC mRNA synthesis was moderately induced by heat, we were unable to identify the ClpC protein. When we analyzed mutants with disruptions in clpB, clpC, or clpE, we found that although the genes are part of the L. lactis heat shock stimulon, the mutants responded like wild-type cells to heat and salt treatments. However, when exposed to puromycin, a tRNA analogue that results in the synthesis of truncated, randomly folded proteins, clpE mutant cells formed smaller colonies than wild-type cells and clpB and clpC mutant cells. Thus, our data suggest that ClpE, along with ClpP, which recently was shown to participate in the degradation of randomly folded proteins in L. lactis, could be necessary for degrading proteins generated by certain types of stress.  (+info)

TY - CONF. T1 - Modeling of nisin production by Lactococcus lactis. AU - Pongtharangkul, Thunyarat. AU - Demirci, Ali. AU - Puri, Virendra. PY - 2007/11/7. Y1 - 2007/11/7. N2 - Nisin, the only FDA approved bacteriocin, is widely used as a natural food preservative against Gram-positive bacteria. It is produced by lactic acid bacteria Lactococcus lactis subsp. lactis during fermentation. Mathematical models of biomass and nisin production during fermentation not only provide information about the kinetic-metabolic nature of nisin, but also facilitate the control and optimization of nisin production. In this study, several models were developed to describe biomass, nisin, and lactic acid production as well as sucrose consumption during nisin fermentation by L. lactis in suspended-cell batch culture. All models were designed so that they contained biologically relevant parameters. The re-modified logistic and Gompertz models proposed in this study adequately described the growth and the validation ...
TY - JOUR. T1 - Metabolic characterization and transformation of the non-dairy Lactococcus lactis strain KF147, for production of ethanol from xylose. AU - Petersen, Kia Vest. AU - Liu, Jianming. AU - Chen, Jun. AU - Martinussen, Jan. AU - Jensen, Peter Ruhdal. AU - Solem, Christian. PY - 2017. Y1 - 2017. N2 - The non-dairy lactic acid bacterium Lactococcus lactis KF147 can utilize xylose as the sole energy source. To assess whether KF147 could serve as a platform organism for converting second generation sugars into useful chemicals, we characterized growth and product formation for KF147 when grown on xylose. In a defined medium KF147 was found to co-metabolize xylose and arginine, resulting in bi-phasic growth. Especially at low xylose concentrations, arginine significantly improved growth rate. To facilitate further studies of the xylose metabolism, we eliminated arginine catabolism by deleting the arcA gene encoding the arginine deiminase. The fermentation product profile suggested two ...
Following treatment with the mutagen N-methyl-N-nitro-N-nitrosoguanidine, three mutants of Lactococcus lactis subsp. lactis biovar diacetylactis CNRZ 483 that produced diacetyl and acetoin from glucose were isolated. The lactate dehydrogenase activity of these mutants was strongly attenuated, and the mutants produced less lactate than the parental strain. The kinetic properties of lactate dehydrogenase of strain CNRZ 483 and the mutants revealed differences in the affinity of the enzyme for pyruvate, NADH, and fructose-1,6-diphosphate. When cultured aerobically, strain CNRZ 483 transformed 2.3% of glucose to acetoin and produced no diacetyl or 2,3-butanediol. Under the same conditions, mutants 483L1, 483L2, and 483L3 transformed 42.0, 78.9, and 75.8%, respectively, of glucose to C4 compounds (diacetyl, acetoin, and 2,3-butanediol). Anaerobically, strain CNRZ 483 produced no C4 compounds, while mutants 483L1, 483L2, and 483L3 transformed 2.0, 37.0, and 25.8% of glucose to acetoin and 2,3-butanediol. In
TY - JOUR. T1 - Draft Genome Sequences of Three Amino Acid-Secreting Lactococcus lactis Strains. AU - Hernandez-Valdes, Jhonatan A.. AU - de Jong, Anne. AU - Kok, Jan. AU - Kuipers, Oscar P.. N1 - Copyright © 2020 Hernandez-Valdes et al.. PY - 2020/4/16. Y1 - 2020/4/16. N2 - Three Lactococcus lactis strains with the ability to secrete various amino acids (leucine, isoleucine, methionine, valine, glutamic acid, and histidine) were sequenced in order to identify the mechanisms involved in the secretion. Amino acids contribute to flavor formation; therefore, bacterial strains with this ability are relevant for the food industry.. AB - Three Lactococcus lactis strains with the ability to secrete various amino acids (leucine, isoleucine, methionine, valine, glutamic acid, and histidine) were sequenced in order to identify the mechanisms involved in the secretion. Amino acids contribute to flavor formation; therefore, bacterial strains with this ability are relevant for the food industry.. KW - ...
A gene encoding 6-phosphogluconate dehydrogenase (6-PGDH, EC 1.1.1.44) was identified from the homofermentative lactic acid bacterium Lactococcus lactis, by complementation of Escherichia coli mutants. The cloned gene was then expressed to high levels in E. coli and the protein purified for kinetic analysis. The enzyme had a Km for 6-phosphogluconate of 15.4±1.4 µM and for NADP of 1.9±0.2 µM at pH 7.5. Sequence comparison of the L. lactis 6-PGDH with the corresponding enzyme derived from the pathogenic protozoan Trypanosoma brucei and sheep liver revealed the substrate-binding residues to be identical in all three species, although the three coenzyme-binding pockets differed slightly. A totally conserved arginine residue (Arg-447), believed to bind the 6-phosphate of substrate, was mutated to lysine, aspartate, alanine or tryptophan. In each case enzyme activity was lost, confirming an essential role for this residue on activity. A second arginine (Arg-34), believed to be critical in binding ...
TY - JOUR. T1 - Two different pathways for D-xylose metabolism and the effect of xylose concentration on the yield coefficient of L-lactate in mixed-acid fermentation by the lactic acid bacterium Lactococcus lactis IO-1. AU - Tanaka, K.. AU - Komiyama, A.. AU - Sonomoto, K.. AU - Ishizaki, A.. AU - Hall, S.. AU - Stanbury, P.. PY - 2002/10. Y1 - 2002/10. N2 - In lactic acid bacteria, pentoses are metabolized via the phosphoketolase pathway, which catalyzes the cleavage of D-xylulose-5-phosphate to equimolar amounts of glyceraldehyde 3-phosphate and acetylphosphate. Hence the yield coefficient of lactate from pentose does not exceed 1.0 mol/mol, while that of Lactococcus lactis IO-1(JCM7638) at high D-xylose concentrations often exceeds the theoretical value. This suggests that, in addition to the phosphoketolase pathway, L. lactis IO-1 may possess another metabolic pathway that produces only lactic acid from xylose. In the present study, the metabolism of xylose in L. lactis IO-1 was deduced ...
The aggregation phenomenon in lactococci has been studied for more than thirty years. It was found to be directly associated with a sex factor and lactose plasmid co-integration event [1] or duplication of the cell wall spanning (CWS) domain of PrtP proteinase [2]. Lactose plasmid conjugation in Lactococcus lactis 712 and in the related strains C2 and ML3, frequently involves plasmid co-integration with a sex factor. Moreover, this phenomenon is often associated with a cell aggregation phenotype and high frequency transfer ability [3-5]. The lactococcal sex factor exists integrated in the chromosome [6], although it can be excised as a closed circular form and lost from the cell [1]. Deletion and over-expression experiments confirmed that CluA is the only sex factor component responsible for aggregation in L. lactis. This 136 kDa surface-bound protein, encoded by the chromosomally located sex factor of Lactococcus lactis subsp. cremoris MG1363, is associated with cell aggregation linked to ...
Epidermal growth factor (EGF) is an important growth factor in regulation of cell proliferation, differentiation, survival and apoptosis. Studies showed that food-grade Lactococcus lactis (L. lactis) and NICE expression system have superior performance in exogenous protein expression. This study aimed to construct and express porcine EGF (pEGF), and use L. lactis as vehicle for producing and delivering pEGF. Furthermore, investigating biological activity of pEGF and exploring applications feasibility of combination effects of L. lactis and pEGF on early weaned piglets production. A recombinant Lactococcus lactis which produced and secreted pEGF at 1000 ng/ml in culture supernatant was generated. Secreted pEGF was a fully biologically active protein, as demonstrated by its capacity to stimulate L929 mouse fibroblast cell line proliferation in vitro. For in vivo study, forty piglets were randomly allocated to control, antibiotic control, empty vector-expressing L. lactis (LL-EV) and pEGF-secreting L.
Listeria monocytogenes is a food-borne intracellular pathogen that mainly infects pregnant and immunocompromised individuals. The pore-forming haemolysin listeriolysin O (LLO), the main virulence factor of Listeria monocytogenes, allows bacteria to escape from the harsh environment of the phagosome to the cytoplasm of the infected cell. This leads to processing of bacterial antigens predominantly through the cytosolic MHC class I presentation pathway. We previously engineered the food-grade bacterium Lactococcus lactis to express LLO and demonstrated an LLO-specific CD8+ response upon immunization of mice with the engineered L. lactis vaccine strains. In the present work, we examined the immune response and protective efficacy of an L. lactis strain co-expressing LLO and a truncated form of the listerial P60 antigen (tP60). Oral immunization revealed no significant protection against listeriosis with L. lactis expressing LLO, tP60 or the combined LLO/tP60. In contrast, intraperitoneal vaccination
Bovine tuberculosis (TBB) is a zoonotic disease distributed worldwide and is of great importance for public health and the livestock industry. Several experimental vaccines against this disease have been evaluated in recent years, yielding varying results. An example is the Bacillus Calmette-Guérin (BCG) vaccine, which has been used extensively in humans and tested in cattle showing mixed results related to protection (0-80%) against Mycobacterium bovis. In this study, we used the food-grade bacterium Lactococcus lactis as an expression system for production of mycobacterial protein Hsp65. For this purpose, the construction of a replicable plasmid in strain NZ9000 L. lactis (pVElepr) was conducted, which expressed the Mycobacterium leprae Hsp65 antigen, and was recognized by traded anti-Hsp65 antibodies. The strain NZ9000-pVElepr was applied to calves that were negative to tuberculin test and the immune response was monitored. The results showed that immune response was not significantly increased in
ID KITH_LACLA Reviewed; 189 AA. AC Q9CHX5; DT 31-OCT-2003, integrated into UniProtKB/Swiss-Prot. DT 01-JUN-2001, sequence version 1. DT 07-JUN-2017, entry version 82. DE RecName: Full=Thymidine kinase {ECO:0000255,HAMAP-Rule:MF_00124}; DE EC=2.7.1.21 {ECO:0000255,HAMAP-Rule:MF_00124}; GN Name=tdk {ECO:0000255,HAMAP-Rule:MF_00124}; OrderedLocusNames=LL0593; GN ORFNames=L184871; OS Lactococcus lactis subsp. lactis (strain IL1403) (Streptococcus OS lactis). OC Bacteria; Firmicutes; Bacilli; Lactobacillales; Streptococcaceae; OC Lactococcus. OX NCBI_TaxID=272623; RN [1] RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=IL1403; RX PubMed=11337471; DOI=10.1101/gr.GR-1697R; RA Bolotin A., Wincker P., Mauger S., Jaillon O., Malarme K., RA Weissenbach J., Ehrlich S.D., Sorokin A.; RT The complete genome sequence of the lactic acid bacterium Lactococcus RT lactis ssp. lactis IL1403.; RL Genome Res. 11:731-753(2001). CC -!- CATALYTIC ACTIVITY: ATP + thymidine = ADP + thymidine 5- CC phosphate. ...
In the present study we examine the changes in the expression of genes of Lactococcus lactis subspecies cremoris MG1363 during growth in milk. To reveal which specific classes of genes (pathways, operons, regulons, COGs) are important, we performed a transcriptome time series experiment. Global analysis of gene expression over time showed that L. lactis adapted quickly to the environmental changes. Using upstream sequences of genes with correlated gene expression profiles, we uncovered a substantial number of putative DNA binding motifs that may be relevant for L. lactis fermentative growth in milk. All available novel and literature-derived data were integrated into network reconstruction building blocks, which were used to reconstruct and visualize the L. lactis gene regulatory network. This network enables easy mining in the chrono-transcriptomics data. A freely available website at http://milkts.molgenrug.nl gives full access to all transcriptome data, to the reconstructed network and to the ...
Since the 1970s, galactose metabolism in Lactococcus lactis has been in debate. Different studies led to diverse outcomes making it difficult to conclude whether galactose uptake was PEP- or ATP-dependent and decide what the exact connection was between galactose and lactose uptake and metabolism. It was shown that some Lactococcus strains possess two galactose-specific systems - a permease and a PTS, even if they lack the lactose utilization plasmid, proving that a lactose-independent PTSGal exists. However, the PTSGal transporter was never identified. Here, with the help of transcriptome analyses and genetic knock-out mutants, we reveal the identities of two low-affinity galactose PTSs. A novel plant-niche-related PTS component Llmg_0963 forming a hybrid transporter Llmg_0963PtcBA and a glucose/mannose-specific PTS are shown to be involved in galactose transport in L. lactis MG1363 ...
BACKGROUND: Lactococcus lactis is used in dairy food fermentation and for the efficient production of industrially relevant enzymes. The genome content and different phenotypes have been determined for multiple L. lactis strains in order to understand intra-species genotype and phenotype diversity and annotate gene functions. In this study, we identified relations between gene presence and a collection of 207 phenotypes across 38 L. lactis strains of dairy and plant origin. Gene occurrence and phenotype data were used in an iterative gene selection procedure, based on the Random Forest algorithm, to identify genotype-phenotype relations. RESULTS: A total of 1388 gene-phenotype relations were found, of which some confirmed known gene-phenotype relations, such as the importance of arabinose utilization genes only for strains of plant origin. We also identified a gene cluster related to growth on melibiose, a plant disaccharide; this cluster is present only in melibiose-positive strains and can be ...
Figure 2. HPV-16 E7-specific serum IgG antibody (1, top) and E7-specific vaginal IgA antibody (2, bottom) levels by placebo groups [cohorts 1 (A, left); 1 × 109 CFU/mL dose, cohorts 2 (B, center); 5 × 109 CFU/mL dose, and cohorts 3 (C, right); 1 × 1010 CFU/mL dose] and vaccine group [cohorts 4 (A, left); 1 × 109 CFU/mL dose, cohorts 5 (B, center); 5 × 109 CFU/mL dose, and cohorts 6 (C, right); 1 × 1010 CFU/mL dose] measured by ELISA at a serum dilution of 1:100 using goat anti-human IgG H&L (HRP) antibody and at a vaginal fluid dilution of 1:10 using goat anti-human IgA alpha chain (HRP) antibody, respectively. Results are expressed with 95% CIs for the following time points: prevaccination (day 0), days 30, 60, 90, and 240. The absorbance of each well was measured at 450 nm. Bars represent the mean ± standard error value of each group. Statistically significant differences are denoted by an asterisk between T60 and T30, T90, and T240 of vaccine groups (*, P ≤ 0.0001; **, P ≤ 0.001; ...
The United States National Institutes of Health has recently pointed out that despite many years of intensive vaccine research and the development of sophisticated effective vaccines, thousands of...
The aminopeptidase P (PepP, EC 3.4.11.9) gene from Lactococcus lactis ssp. lactis DSM 20481 was cloned, sequenced and expressed recombinantly in E. coli BL21 (DE3) for the first time. PepP is involved in the hydrolysis of proline-rich proteins and, thus, is important for the debittering of protein hydrolysates. For accurate determination of PepP activity, a novel gas chromatographic assay was established. The release of L-leucine during the hydrolysis of L-leucine-L-proline-L-proline (LPP) was examined for determination of PepP activity. Sufficient recombinant PepP production was achieved via bioreactor cultivation at 16 °C, resulting in PepP activity of 90 μkatLPP Lculture-1. After automated chromatographic purification by His-tag affinity chromatography followed by desalting, PepP activity of 73.8 μkatLPP Lculture-1 was achieved. This was approximately 700-fold higher compared to the purified native PepP produced by Lactococcus lactis ssp. lactis NCDO 763 as described in literature. The molecular
The main purpose of this study was to evaluate the antimicrobial activity of twenty-one bacteriocinogenic lactic acid bacteria (12 strains of Lactococcus lactis subsp. lactis, 4 strains of Lactobacillus gasseri, 3 strains of Lb. helveticus and 2 strains of Lb. acidophilus, LAB) against 28 Staphylococcus and 33 Enterococcus strains able to produce tyramine, putrescine, 2-phenylethylamine and cadaverine. The antimicrobial activity of cell-free supernatants (CFS) from tested LAB was examined by an agar-well diffusion assay. Nine out of twenty-one strains (33%) showed the inhibitory effect on tested enterococci and staphylococci, namely 9 strains of Lactococcus lactis subsp. lactis. The diameters of inhibition zones ranged between 7 mm and 14 mm. The biggest diameter of 14 mm inhibition was obtained with the CFSs from strains CCDM 670 and CCDM 731 on Enterococcus sp. E16 and E28. The cell-free supernatants from Lactococcus lactis subsp. lactis CCDM 71 and from Lactococcus lactis subsp. lactis CCDM ...
TY - JOUR. T1 - Genomic features of Lactococcus lactis IO-1, a lactic acid bacterium that utilizes xylose and produces high levels of L-lactic acid. AU - Shimizu-Kadota, Mariko. AU - Kato, Hiroaki. AU - Shiwa, Yuh. AU - Oshima, Kenshiro. AU - Machii, Miki. AU - Araya-Kojima, Tomoko. AU - Zendo, Takeshi. AU - Hattori, Masahira. AU - Sonomoto, Kenji. AU - Yoshikawa, Hirofumi. PY - 2013. Y1 - 2013. N2 - Lactococcus lactis IO-1 (JCM7638) produces L-lactic acid predominantly when grown at high xylose concentrations, and its utilization is highly desired in the green plastics industry. Therefore it is worthwhile studying its genomic traits. In this study, we focused on (i) genes of possible horizontal transfer derivation (prophages, the nisin-sucrose transposon, and several restrictionmodification systems), and (ii) genes for the synthetic pathways of amino acids and vitamins in the IO-1 genome. In view of the results of this analysis, we consider their meanings in strain IO-1.. AB - Lactococcus ...
Lactococcus lactis is a Gram-positive bacterium used extensively in the production of buttermilk and cheese, but has also become famous as the first genetically modified organism to be used alive for the treatment of human disease. L. lactis cells are cocci that group in pairs and short chains, and, depending on growth conditions, appear ovoid with a typical length of 0.5 - 1.5 µm. L. lactis does not produce spores (nonsporulating) and are not motile (nonmotile). They have a homofermentative metabolism, meaning they produce lactic acid from sugars. Theyve aso been reported to produce exclusive L-(+)-lactic acid. However, reported D-(−)-lactic acid can be produced when cultured at low pH. The capability to produce lactic acid is one of the reasons why L. lactis is one of the most important microorganisms in the dairy industry. Based on its history in food fermentation, L. lactis has generally recognized as safe (GRAS) status with few case reports of being an opportunistic pathogen. L. lactis ...
Lactococcus lactis, a Gram-positive lactic acid bacterium and a classical starter for the manufacture of cheeses, can be used as a cell factory to produce proteins of interest [1-6]. As a long known innocuous, Generally Recognised As Safe (GRAS) food-grade species [7], L. lactis is an interesting host to produce proteins of medical interest [2, 4, 5]. Compared to Escherichia coli, the advantage of L. lactis is that it does not produce endotoxin (lipopolysaccharide) [2, 4, 5] which has to be removed from protein preparations before medical use [8]. In contrast to Bacillus subtilis, L. lactis secretes only one major endogenous protein, Usp45, and no proteases [9]: a strategy combining production and secretion of heterologous proteins in L. lactis is thus interesting as it facilitates protein purification from the culture medium [4]. As secreted heterologous proteins can be degraded by the lactococcal surface protease HtrA, protein yield can be improved by the use of a mutant strain devoid of ...
Although folate is naturally produced in E.coli, the folate biosynthesis pathway in the bacteria Lactococcus lactis has been more heavily characterized and studied. There are six major enzymes involved in folate synthesis, which, in L.lactis, are contained in five genes: folB, folKE, folP, folC, and folA[1]. The first four, which we have chosen to focus on, are located in a gene cluster approximately 4.4kb long. Weve chosen not to focus on folA for the time being because folA encodes an enzyme which turns one form of folate (dihydrofolate) into another form of folate(tetrahydrofolate). Since our assay would detect both types of folate as part of the total folate production, folA was not a prime target for overexpression of folate. In previous studies, this folate gene cluster has been successfully transformed into the folate-consuming bacteria L.gasseri, turning the bacteria in to folate-producers[3]. Therefore, we have chosen to also use the folate operon from L.lactis, which also offers the ...
Although folate is naturally produced in E.coli, the folate biosynthesis pathway in the bacteria Lactococcus lactis has been more heavily characterized and studied. There are six major enzymes involved in folate synthesis, which, in L.lactis, are contained in five genes: folB, folKE, folP, folC, and folA[1]. The first four, which we have chosen to focus on, are located in a gene cluster approximately 4.4kb long. Weve chosen not to focus on folA for the time being because folA encodes an enzyme which turns one form of folate (dihydrofolate) into another form of folate(tetrahydrofolate). Since our assay would detect both types of folate as part of the total folate production, folA was not a prime target for overexpression of folate. In previous studies, this folate gene cluster has been successfully transformed into the folate-consuming bacteria L.gasseri, turning the bacteria in to folate-producers[3]. Therefore, we have chosen to also use the folate operon from L.lactis, which also offers the ...
How was I to know?. Lactococcus lactis is part of my culture and heritage. Does that make me uncultured for not knowing about this cultures importance, or, to be honest, not knowing about it at all? L.lactis is the microbe which changes milk into many varieties of cheese.. The Assembly in my fair state, Wisconsin, has passed a bill designating Lactococcus lactis as our official state microbe. Since our State Senate has yet to vote, Lactococcus is lobbying hard. How many other bacterium have their own home pages? (The Lactococcus lactis Home Page). At the risk of sounding like a disloyal cheesehead, I am a bit upset about the designation. The fact that my state has been made a laughing stock by the media doesnt faze me. Living in a place where people don large wedges of foam cheese on their heads, I dont fret much about the states image.. No, I am upset because the candidate that I am backing for state designation is languishing in some committee over in Madison. How can our state go ...
Our Probiotic Night Cream is virtually a miracle treatment for damaged skin and has several high-end ingredients that have been developed over years of testing and research. What is Probiotic Technology? Probiotics are bacteria that are in some form beneficial to us. Our Probiotic Night Cream contains a biotechnologically obtained suspension from the bacterium Lactococcus lactis that contains cytoplasm and cell wall fragments which are highly effective in helping the skin to heal and to remain healthy and younger appearing. Some benefits of our Probiotic Technology are: • Induces production of key ingredients of skin quality and differentiation resulting in healthier skin. • Improves keratinocyte cohesion resulting in a more robust skin barrier that protects the skin. • Increases the production of antimicrobial peptides which fight harmful bacteria. • Promotes skin renewal (old cells being replaced with new and healthier cells). In addition to our Probiotic Technology, weve also added ...
Until now, data on HMA1, HMA6 and HMA8 functions were based on indirect measures; the absence of biochemical data can be explained by the difficulty to produce high amounts of these membrane proteins in an active form. The CEA-PM project dedicated to the screening of alternative expression system for membrane proteins showed that the bacteria Lactococcus lactis is particularly suitable for the production of plant membrane proteins, including the HMAs [2, 3] ...
Applications are invited for the position of Junior Research Fellow on SERB sponsored project entitled Evaluation of immunogenicity and protective efficacy of bivalent vaccine candidate against Shigella and Salmonella using food grade bacteria Lactococcus lactis under the supervision of Dr. Priti Desai, Institute of Advanced Research, Koba Institutional Area, Koba, Gandhinagar-382 007. ...
p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.,/p> ,p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.,/p> ,p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).,/p> ,p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x,sup>64,/sup> + x,sup>4,/sup> + x,sup>3,/sup> + x + 1. The algorithm is described in the ISO 3309 standard. ,/p> ,p class=publication>Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.,br /> ,strong>Cyclic redundancy and other checksums,/strong>,br /> ,a href=http://www.nrbook.com/b/bookcpdf.php>Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993),/a>),/p> Checksum:i ...
Ubiquinone (UQ), also called coenzyme Q, and plastoquinone (PQ) are electron carriers in oxidative phosphorylation and photosynthesis, respectively. The quinoid nucleus of ubiquinone is derived from the shikimate pathway; 4-hydroxybenzoate is directly formed from chorismate in bacteria, while it can be formed from either chorismate or tyrosine in yeast. The following biosynthesis of terpenoid moiety involves reactions of prenylation, decarboxylation, and three hydroxylations alternating with three methylations. The order of these reactions are somewhat different between bacteria and yeast. Phylloquinone (vitamin K1), menaquinone (vitamin K2), and tocopherol (vitamin E) are fat-soluble vitamins. Phylloquinone is a compound present in all photosynthetic plants serving as a cofactor for photosystem I-mediated electron transport. Menaquinone is an obligatory component of the electron-transfer pathway in bacteria ...
CodY, a pleiotropic transcriptional regulator conserved in low G+C species of Gram-positive bacteria, was previously described to be the central regulator of proteolysis in Lactococcus lactis. In this study, over 100 potential CodY targets were identified by DNA-microarray analysis. Complementary tr …
Protein synthesis is a template polymerization process composed by three main steps: initiation, elongation, and termination. During translation, ribosomes are engaged into polysomes whose size is used for the quantitative characterization of translatome. However, simultaneous transcription and translation in the bacterial cytosol complicates the analysis of translatome data. We established a procedure for robust estimation of the ribosomal density in hundreds of genes from Lactococcus lactis polysome size measurements. We used a mechanistic model of translation to integrate the information about the ribosomal density and for the first time we estimated the protein synthesis rate for each gene and identified the rate limiting steps. Contrary to conventional considerations, we find significant number of genes to be elongation limited. This number increases during stress conditions compared to optimal growth and proteins synthesized at maximum rate are predominantly elongation limited. Consistent with
Recent advances in malaria control efforts have led to an increased number of national malaria control programmes implementing pre-elimination measures and demonstrated the need to develop new tools to track and control malaria transmission. Key to understanding transmission is monitoring the prevalence and immune response against the sexual stages of the parasite, known as gametocytes, which are responsible for transmission. Sexual-stage specific antigens, Pfs230 and Pfs48/45, have been identified and shown to be targets for transmission blocking antibodies, but they have been difficult to produce recombinantly in the absence of a fusion partner. Regions of Pfs48/45 and Pfs230 known to contain transmission blocking epitopes, 6C and C0, respectively, were produced in a Lactococcus lactis expression system and used in enzyme linked immunosorbent assays to determine the seroreactivity of 95 malaria patients living in the Central Region of Ghana. Pfs48/45.6C and Pfs230.C0 were successfully produced in L.
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L. lactis is of crucial importance for manufacturing dairy products, such as buttermilk and cheeses. When L. lactis ssp. lactis is added to milk, the bacterium uses enzymes to produce energy molecules (ATP), from lactose. The byproduct of ATP energy production is lactic acid. The lactic acid produced by the bacterium curdles the milk that then separates to form curds, which are used to produce cheese.[11] Other uses that have been reported for this bacterium include the production of pickled vegetables, beer or wine, some breads, and other fermented foodstuffs, such as soymilk kefir, buttermilk, and others.[12] L. lactis is one of the best characterized low GC Gram positive bacteria with detailed knowledge on genetics, metabolism and biodiversity.[13][14]. L. lactis is mainly isolated from either the dairy environment or plant material.[15][16][17] Dairy isolates are suggested to have evolved from plant isolates through a process in which genes without benefit in the rich medium milk were either ...
Citrate is present in only low levels 0.15-0.2 in milk and O-type starters do not include the citrate utilizing Lc. lactis subsp. lactis biovar diacetylactis.
Bidirectionally degrades single-stranded DNA into large acid-insoluble oligonucleotides, which are then degraded further into small acid-soluble oligonucleotides.
Raw milk is often described as a major source of lactic acid bacteria for indigenous lactic starter. These indigenous starters contribute to the sensorial quality of cheese. Raw milk, rich in Lactoccocus lactis may therefore be very interesting for the cheese making. Currently, the most commonly used methods to differentiate lactic acid bacteria, and particularly the closely related phenotypes Lactococcus and Enterococcus, are based on DNA sequencing, but the cost and time required for these analytical methods hinder their use for rapid screening of raw material. The present study therefore proposes a simple alternative method to identify and discriminate against Lactococcus and Enterococcus, at the genus, but also at the species level, that is based on collecting near infrared spectra directly from bacterial colonies in Petri dishes. The infrared spectra of 280 strains of Lactococcus and Enterococcus cultured on solid media were collected by using a spectrometer with a wavelength range of 908 ...
Background: Vitamin K2 (menaquinone, MK-n) is a lipid-soluble vitamin that functions as a carboxylase co-factor for maturation of proteins involved in many vital physiological processes in humans. Notably, long-chain vitamin K2 is produced by bacteria, including some species and strains belonging to the group of lactic acid bacteria (LAB) that play important roles in food fermentation processes. This study was performed to gain insights into the natural long-chain vitamin K2 production capacity of LAB and the factors influencing vitamin K2 production during cultivation, providing a basis for biotechnological production of vitamin K2 and in situ fortification of this vitamin in food products. Results: We observed that six selected Lactococcus lactis strains produced MK-5 to MK-10, with MK-8 and MK-9 as the major MK variant. Significant diversities between strains were observed in terms of specific concentrations and titres of vitamin K2. L. lactis ssp. cremoris MG1363 was selected for more ...
One of the great achievements of the omics revolution is the development of high throughput functional genomics approaches. Notably transcriptomics studies have been instrumental in analyzing the response of LAB to different environments and over 100 papers have been published addressing a variety of stresses, growth conditions and culturing regimens. Among the most important practical discoveries was the finding that Lactococcus lactis and other LAB, when provided with the appropriate cofactors, such as heme, could use alternative electron acceptors and hence respire rather than ferment [82]. Following the first description of the use of molecular oxygen by Lactococcus lactis MG1363 [83], a variety of studies have followed that have been reviewed recently [84]. Transcriptional studies allowed to identify the genes involved in the use of molecular oxygen that resulted in a wide range of applications, notably faster growth and higher yield of starter cultures [85]. Biochemical studies confirmed ...
Gram-positive sphere. Forms short chains. A common contaminant of milk products, causing souring. Excellent for classroom demonstrations. Incubate at 37°C. On brain heart infusion …
Knowledge of phage-host interactions at a fundamental level is central to the design of rational strategies for the development of phage-resistant strains that may be applied in industrial settings. Phages infecting lactic acid bacteria, in particular Lactococcus lactis and Streptococcus thermophilus, negatively impact on dairy fermentation processes with serious economic implications. In recent years a wealth of information on structural protein assembly and topology has become available relating to phages infecting Escherichia coli, Bacillus subtilis and Lactococcus lactis, which act as models for structural analyses of dairy phages. In this review, we explore the role of model tailed phages, such as T4 and SPP1, in advancing our knowledge regarding interactions between dairy phages and their hosts. Furthermore, the potential of currently investigated dairy phages to in turn serve as model systems for this particular group of phages is discussed.
Knowledge of phage-host interactions at a fundamental level is central to the design of rational strategies for the development of phage-resistant strains that may be applied in industrial settings. Phages infecting lactic acid bacteria, in particular Lactococcus lactis and Streptococcus thermophilus, negatively impact on dairy fermentation processes with serious economic implications. In recent years a wealth of information on structural protein assembly and topology has become available relating to phages infecting Escherichia coli, Bacillus subtilis and Lactococcus lactis, which act as models for structural analyses of dairy phages. In this review, we explore the role of model tailed phages, such as T4 and SPP1, in advancing our knowledge regarding interactions between dairy phages and their hosts. Furthermore, the potential of currently investigated dairy phages to in turn serve as model systems for this particular group of phages is discussed.
SWISS-MODEL Repository entry for P96790 (KUP1_LACLM), Putative potassium transport system protein kup 1. Lactococcus lactis subsp cremoris (strain MG1363)
SWISS-MODEL Repository entry for A2RNR2 (RL333_LACLM), 50S ribosomal protein L33 3. Lactococcus lactis subsp cremoris (strain MG1363)
The functions of starters in dairy fermentations and the importance of the antimicrobial agents produced by starters including undissociated lactic acid is discussed
Construction of specific erythromycin resistance mutations in the temperate lactococcal bacteriophage TP901-1 and their use in studies of phage biology ...
The potential of the thymidylate synthase thyA gene cloned from Lactococcus lactis subsp. lactis as a possible alternative selectable marker gene to antibiotic resistance markers has been examined. The thyA mutation is a recessive lethal one; thyA mutants cannot survive in environments containing low amounts of thymidine or thymine (such as Luria-Bertani medium) unless complemented by the thyA gene. The cloned thyA gene was strongly expressed in L. lactis subsp. lactis, Escherichia coli, Rhizobium meliloti, and a fluorescent Pseudomonas strain. In addition, when fused to a promoterless enteric lac operon, the thyA gene drove expression of the lac genes in a number of gram-negative bacteria. In transformation experiments with thyA mutants of E. coli and conjugation experiments with thyA mutants of R. meliloti, the lactococcal thyA gene permitted selection of transformants and transconjugants with the same efficiency as did genes for resistance to ampicillin, chloramphenicol, or tetracycline. ...
Define nisin. nisin synonyms, nisin pronunciation, nisin translation, English dictionary definition of nisin. n. A bacteriocin produced by the bacterium Lactococcus lactis and used as a preservative in dairy products and other foods and in cosmetics
We report here the complete EM structure of a lactococcal phage determined by single-particle analysis. Phage p2 is the flagship of the 936 group, which are the most prevalent lactococcal phages in industrial dairy fermentations worldwide. This phage is highly virulent and requires Ca2+ to infect specific L. lactis cells, which is in contrast with the less prevalent lactococcal phages belonging to the P335 subgroup II (e.g., TP901-1 and Tuc2009) (15).. The shape of p2 icosahedral capsid is comparable to that of other siphophages. The capsid pentons are, however, more prominent than those of phage TP901-1 but akin to those of coliphage HK97. In contrast, the size of the p2 portal protein (ORF-4) is among the smallest, particularly in comparison to those of phage TP901-1 and Bacillus phage SPP1 (Table 3). The two putative head completion proteins of p2 (ORF-8 and ORF-9) are comparable to those of phage SPP1. The fit of the SPP1 portal and head completion protein 1 (gp15) into the p2 connector EM ...
In Lactococcus lactis the enzymes phosphofructokinase (PFK), pyruvate kinase (PK) and lactate dehydrogenase (LDH) are uniquely encoded in the las operon and we here apply Metabolic Control Analysis to study the role of this organisation. Earlier work showed that LDH at wildtype level has zero control on glycolysis and growth rate but high negative control on formate production. We find that PFK and PK have zero control on glycolysis and growth rate at the wildtype enzyme level but both enzymes exert strong positive control on the glycolytic flux at reduced activities. PK has high positive control on formate and acetate production, whereas PFK has no control on these fluxes. Decreased expression of the entire las operon resulted in a strong decrease in growth rate and the glycolytic flux; at 53% expression of the las operon the glycolytic flux was reduced to 44% and the flux control coefficient increased towards 3. Increased las expression resulted in a slight decrease in the glycolytic flux. At ...
The E7 protein of human papillomavirus type 16 was produced in Lactococcus lactis. Secretion allowed higher production yields than cytoplasmic production. In stationary phase, amounts of cytoplasmic E7 were reduced, while amounts of secreted E7 increased, suggesting a phase-dependent intracellular proteolysis. Fusion of E7 to the staphylococcal nuclease, a stable protein, resulted in a highly stable cytoplasmic protein. This work provides new candidates for development of viral screening systems and for oral vaccine against cervical cancer. ...
Cheese in a sack is a specific Croatian indigenous semi hard cheese. The main characteristic of this cheese is ripening and keeping in sheeps clothing (Mišina), which gives it a typical and very appreciated, spicy flavor and aroma. Cheeses obtained by similar technology and ripening are also produced in Turkey (Tulum cheese), Lebanon (darfiyeh), Bosnia and Herzegovina (sack cheese), Montenegro (sack cheese) and eastern Algeria (bouhezza cheese). In Croatia, cheese in a sack has been produced traditionally for many years, without the use of commercial starter cultures, which can result in differences in taste, consistency and microbiological quality.Therefore, there is a growing trend of finding and selecting of new natural indigenous potential starter cultures from traditional products, which will be adapted to the growth in certain specific traditional products, and will have such metabolic capabilities that will significantly affect the quality and safety of products. The hypothesis is that ...
Lactic streptococci, classically regarded as homolactic fermenters of glucose and lactose, became heterolactic when grown with limiting carbohydrate concentrations in a chemostat. At high dilution rates (D) with excess glucose present, about 95% of the fermented sugar was converted to l-lactate. However, as D was lowered and glucose became limiting, five of the six strains tested changed to a heterolactic fermentation such that at D = 0.1 h(-1) as little as 1% of the glucose was converted to l-lactate. The products formed after this phenotypic change in fermentation pattern were formate, acetate, and ethanol. The level of lactate dehydrogenase, which is dependent upon ketohexose diphosphate for activity, decreased as fermentation became heterolactic with Streptococcus lactis ML(3). Transfer of heterolactic cells from the chemostat to buffer containing glucose resulted in the nongrowing cells converting nearly 80% of the glucose to l-lactate, indicating that fine control of enzyme activity is an ...
Lactic acid bacteria are used as starter cultures in dairy fermentations. Starter cultures employed in cheese manufacturing encounter many different environmental conditions during their production in starter-producing companies and during their application in the cheese manufacturing industry. To optimally perform, microorganisms have to continuously adapt to these changing conditions. Bacteria adjust their physiology to maximize their fitness to the condition they are in, at the cost of being less fit under different conditions. Microbial fitness under different conditions is determined by rate of adaptation including proteome degradation, production and renewal. Adaptation and turnover of the proteome is constrained by the availability of resources and/or cellular energy levels, which results in trade-offs during optimized growth followed by adaptation: the optimization of one trait usually correlates with decreased performance of another trait. In this project, we investigate, by proteomics, ...
Kikuchi, T.; Desmazeaud, M.; Bergere, J.L., 1973: Proteolytic action of lactic streptococci. I. The action of mesophilic lactic streptococci on N constituents of milk
Staphylococcus aureus is an important pathogen that causes various infections in medical facilities. However, resistance to multiple drugs has made this infection difficult to manage. Thus, new therapeutic strategies are urgently needed to solve this worldwide public health problem. The Streptococcus lactis L16 strain was isolated from the fermented hot chili sauce. To explore whether it can be used as a protective agent against S. aureus infection, we designed a mouse model of S. aureus infection to evaluate the therapeutic potency of S. lactis. Mice were grouped into pre-(P) and post-(T) S. aureus infection groups following oral administration of S. lactis L16. The protection and treatment effects were assessed by examining body weight, internal organ weight, serum cytokines and intestinal secretory IgA alternations. Oral administration of the S. lactis L16 strain reduced the loss of body weight in mice post-infection and alleviated infection-induced hepatomegaly. In
B. lactis is a widely used probiotic and is considered a champion among the good bacteria. It has been reclassified by Masco et al. (2004) and has been found to be related to Bifidobacterium animalis at the sup specie level, B. animalis subsp. Lactis. B. lactis is a common inhabitant of the colon and intestines of humans and animals. The use of B. lactis in diets has shown beneficial effects in the treatment of various gluten-based enteropathies. Celiac disease is an autoimmune disorder which is aggravated by the consumption of gluten-based food products.. According to Lindfors et al. (2008), the oral intake of B. lactis based-probiotics protects the intestinal cells from the toxic effects of gluten enteropathies and inflammatory reactions. The regular consumption of B. lactis aids in controlling abnormal weight gain and diabetes in animal models. Stenman et al. (2014) administered a probiotic based on Bifidobacterium animalis ssp. Lactis 420 to mice, which showed positive results in ...
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Accepted name: trehalose 6-phosphate phosphorylase. Reaction: α,α-trehalose 6-phosphate + phosphate = glucose 6-phosphate + β-D-glucose 1-phosphate. Systematic name: α,α-trehalose 6-phosphate:phosphate β-D-glucosyltransferase. Comments: The enzyme from Lactococcus lactis is specific for trehalose 6-phosphate. Differs from EC 2.4.1.64, α,α-trehalose phosphorylase, in that trehalose is not a substrate.. Links to other databases: BRENDA, EXPASY, KEGG, Metacyc, CAS registry number: 403512-51-6. References:. 1. Andersson, U., Levander, F. and Radstrom, P. Trehalose 6-phosphate phosphorylase is part of a novel metabolic pathway for trehalose utilization in Lactococcus lactis. J. Biol. Chem. 276 (2001) 42707-42713. [PMID: 11553642]. ...
Recent advances in malaria control efforts have led to an increased number of national malaria control programmes implementing pre-elimination measures and demonstrated the need to develop new tools to track and control malaria transmission. Key to understanding transmission is monitoring the prevalence and immune response against the sexual stages of the parasite, known as gametocytes, which are responsible for transmission. Sexual-stage specific antigens, Pfs230 and Pfs48/45, have been identified and shown to be targets for transmission blocking antibodies, but they have been difficult to produce recombinantly in the absence of a fusion partner. Regions of Pfs48/45 and Pfs230 known to contain transmission blocking epitopes, 6C and C0, respectively, were produced in a Lactococcus lactis expression system and used in enzyme linked immunosorbent assays to determine the seroreactivity of 95 malaria patients living in the Central Region of Ghana. Pfs48/45.6C and Pfs230.C0 were successfully produced in L.
The expression of vaccine antigens in lactic acid bacteria (LAB) is a safe and cost-effective alternative to traditional expression systems. In this study, we investigated i) the expression of Human papillomavirus type 16 (HPV-16) L1 major capsid protein in the model LAB Lactococcus lactis and ii) the ability of the resulting recombinant strain to produce either capsomer-or virus-like particles (VLPs). HPV-16 L1 gene was cloned into two vectors, pCYT and pSEC, designed for controlled intra- or extracellular heterologous expression in L. lactis, respectively. The capacity of L. lactis harboring either pCYT:L1 or pSEC:L1 plasmid to accumulate L1 in the cytoplasm and supernatant samples was confirmed by Western blot assays. Electron microscopy analysis suggests that, L1 protein produced by recombinant lactococci can self-assemble into structures morphologically similar to VLPs intracellularly. The presence of conformational epitopes on the L. lactis-derived VLPs was confirmed by ELISA using an anti-HPV16
Carunchia Whetstine, M. E.; M. A. Drake, J. R Broadbent, and McMahon. 2006. Enhanced nutty flavor formation in Cheddar cheese made with a malty Lactococcus lactis adjunct culture. J. Dairy Sci. 89: 3277-3284. ...
His expertise centers around the molecular biology and physiology of bacteria, with a special focus on lactic acid bacteria, probiotics, and the intestinal microbiota. He is strongly involved in the genomics driven work related to understanding the molecular mechanisms of environmental adaptation in Lactobacillus plantarum and Lactococcus lactis, focusing on adaptation to the conditions encountered in the oro-gastrointestinal tract and conditions encountered during (industrial) dairy fermentation, respectively. In recent years he has expanded his field to the (post-genomic) molecular analysis of the mechanisms of communication between intestinal bacteria and the host mucosa. Michiel Kleerebezem has (co-) authored more than 200 peer-reviewed publications and is inventor on more than 25 patent applications ...
Undefined mesophilic mixed (DL-type) starter cultures are composed of predominantly Lactococcus lactis subspecies and 1-10% Leuconostoc spp. The composition of the Leuconostoc population in the starter culture ultimately affects the characteristics and the quality of the final product. The scientific basis for the taxonomy of dairy relevant leuconostocs can be traced back 50 years, and no documentation on the genomic diversity of leuconostocs in starter cultures exists. We present data on the Leuconostoc population in five DL-type starter cultures commonly used by the dairy industry. The analyses were performed using traditional cultivation methods, and further augmented by next-generation DNA sequencing methods. Bacterial counts for starter cultures cultivated on two different media, MRS and MPCA, revealed large differences in the relative abundance of leuconostocs. Most of the leuconostocs in two of the starter cultures were unable to grow on MRS, emphasizing the limitations of culture-based methods
In the present study, we investigated the bacterial diversity of aMasi, a traditional South African fermented milk product, by 16S rRNA clone library and Denaturing Gradient Gel Electrophoresis (DGGE) analysis. Two hundred and eighty two clones from clone library were isolated and identified from aMasi, prepared from the milk of four cows from one herd in the EkuPindiseni Community, North West of Hluhluwe-iMfolozi Park in KwaZulu-Natal Province. The majority of the identified sequences corresponded to lactic acid bacteria (LAB), with the genus Lactococcus as major representative. The species Lactococcus lactis accounted for 179 of the identified clones. In addition, several species of Lactobacillus, Leuconostoc and Enterococcus were detected. Furthermore, several clones belonging to Acinetobacter, Aeromonas and genera within the Enterobacteriaceae were detected. It is important to note that human pathogens such as Klebsiella pneumoniae were identified in aMasi in the present study. Conversely, ...
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Conjugation is a common mode of genetic transfer among the lactic acid bacteria. In an effort to exploit conjugation as a means of lactococcal strain development, we have characterized the transfer re
Vahora, G.Y.; Ernstrom, C.A.; Post, F.J., 1973: Production of proteolytic activity by selected strains of lactic streptococci in skim milk and a whey medium
Lactococcus garvieae is a pathogen that causes septicemia in fish and serious damage to fish aquaculture worldwide, but recently has been isolated from human, ruminant, water sources and several food matrix. In the present work we monitored the population structure of L. garvieae strains, including isolates of dairy, fish, meat, vegetable and cereal origin. This bacterial collection was explored using molecular polyphasic approach comprising PCR-ribotyping, Rep and RAPD-PCR analyses and multilocus restriction typing (MLRT) carried out on six partial genes. This approach allowed high-resolution cluster analysis in which two major groups were distinguishable: one group included dairy isolates, the other group meat isolates. Unexpectedly, some strains coming from fish grouped with dairy isolates whereas the other with meat isolates. Likewise, strains isolated from vegetables allocated between the two main groups. These findings revealed high variability within the species at both gene and genome ...
Structure and Conformational Dynamics of the Domain 5 RNA Hairpin of a Bacterial Group II Intron Revealed by Solution Nuclear Magnetic Resonance and Molecular Dynamics ...
Abstract: Multidrug efflux pumps present a challenge to the treatment of bacterial infections, making it vitally important to understand their mechanism of action. Here, we investigate the nature of substrate binding within Lactococcus lactis LmrP, a prototypical multidrug transporter of the major facilitator superfamily. We determined the crystal structure of LmrP in a ligand-bound outward-open state and observed an embedded lipid in the binding cavity of LmrP, an observation supported by native mass spectrometry analyses. Molecular dynamics simulations suggest that the anionic lipid stabilizes the observed ligand-bound structure. Mutants engineered to disrupt binding of the embedded lipid display reduced transport of some, but not all, antibiotic substrates. Our results suggest that a lipid within the binding cavity could provide a malleable hydrophobic component that allows adaptation to the presence of different substrates, helping to explain the broad specificity of this protein and ...
Im a modeller, specialized in kinetic modeling of biochemical networks. My focus in the SysMO-LAB consortium is on creating models of Lactococcus lactis glycolysis and couple this to other related lactic acid bacteria like Streptococcus pyogenes and Enterococcus faecalis. Besides kinetic modeling, Im also interested in combining various modeling techniques (genome-scale modeling, qualitative modeling). ...
Im a modeller, specialized in kinetic modeling of biochemical networks. My focus in the SysMO-LAB consortium is on creating models of Lactococcus lactis glycolysis and couple this to other related lactic acid bacteria like Streptococcus pyogenes and Enterococcus faecalis. Besides kinetic modeling, Im also interested in combining various modeling techniques (genome-scale modeling, qualitative modeling). ...
Excerpt from my article for Fermentation Magazine, access the full article here. One of the raw milk cheese I used to make for Pholia Farm. A Romano style goat, aged 1 year. Long before packets of freeze-dried cultures for milk existed, there were dairy ferments. Cheese, yogurt, and kefir are probably second only to beer…
Vermassen A, Dordet-Frisoni E, de LaFoye A, Micheau P, Laroute V, Leroy S, Talon R. 2016. Adaptation of Staphylococcus xylosus to nutrients and osmotic stress in a salted meat model. Front Microbiol. Feb 7;87.. Morin M, Ropers D, Letisse F, Laguerre S, Portais J-C, Enjalbert B, Cocaign-Bousquet M. 2016. The post-transcriptional regulatory system CSR controls the balance of metabolic pools in upper glycolysis of Escherichia coli. Mol Microbiol ; doi: 10.1111/mmi.13343. Castelain M., Duviau M-P, Canette A, Schmitz P, Loubière P, Cocaign-Bousquet M, Piard J-C, Mercier-Bonin M. 2016. The nanomechanical properties of Lactococcus lactis pili are conditioned by the polymerized backbone pilin. PLoS ONE 11(3): e0152053. doi:10.1371/journal.pone.0152053. Esquerré T, Turlan C, Bouvier M, Carpousis AJ, Girbal L, Cocaign-Bousquet M. 2016. The Csr system regulates genome-wide mRNA stability and transcription and thus gene expression in Escherichia coli. Sci Rep. 2016 Apr 26;6:25057. doi: ...
2.A.105 The Mitochondrial Pyruvate Carrier (MPC) Family. The transport of pyruvate, the end product of glycolysis, into mitochondria is an essential process that provides the organelle with a major oxidative fuel. Herzig et al. (2012) reported that MPC is a heterocomplex formed by two members of the MPC family that are conserved from yeast to mammals. Members of the MPC family are in the inner mitochondrial membrane, and yeast mutants lacking MPC proteins show severe defects in mitochondrial pyruvate uptake. Coexpression of mouse MPC1 and MPC2 in Lactococcus lactis promoted transport of pyruvate across the membrane (Herzig et al., 2012). Yeast MPC proteins with an odd number of transmembrane segments and a matrix-exposed N-terminus are imported by the carrier pathway, using the receptor Tom70, small TIM chaperones, and the TIM22 complex. The TIM9.10 complex chaperones MPC proteins through the mitochondrial intermembrane space using conserved hydrophobic motifs that are also required for the ...
Introducing The Ordinary Buffet, a multi-peptide serum that targets multiple signs of aging - dryness, fine lines, wrinkles, dullness, and uneven texture. The formula combines a comprehensive array of studied techonologies to combat aging. The technologies are Matrixyl™ 3000 peptide complex (with palmitoyl tetrapeptide-7), Matrixyl™ synthe6™ peptide complex (with palmitoyl tripeptide-38), SYN™-AKE peptide complex (with dipeptide diaminobutyroyl benzylamide diacetate), Relistase peptide complex (with acetylarginyltryptophyl diphenylglycine), ARGIRELOX™ peptide complex (with acetyl hexapeptide-8, pentapeptide-18), Probiotic complex (with lactococcus lactis lysate), in a base of 11 skin-friendly amino acids and multiple hyaluronic acid complexes. The total concentration of these technologies in the formula by weight is a whopping 25.1%!. Start incorporating anti-aging serums as early as in your 20s!. Note from the The Ordinary: It is best to not use Peptides in the same routine as the ...
This formula combines a comprehensive array of studied technologies to target multiple signs of ageing at once. The technologies are Matrixyl 3,000 peptide complex (with palmitoyl tetrapeptide-7), Matrixyl Synthe6 peptide complex (with palmitoyl tripeptide-38), Syn-Ake peptide complex (with dipeptide diaminobutyroyl benzylamide diacetate), Relistase peptide complex (with acetylarginyltryptophyl diphenylglycine), Argirelox peptide complex (with acetyl hexapeptide-8, pentapeptide-18), Probiotic complex (with lactococcus lactis lysate), in a base of 11 skin-friendly amino acids and multiple hyaluronic acid complexes. The total concentration of these technologies in the formula by weight is 25.1%. (References are made to third-party trademarks in this description. All such trademarks are owned by their respective owners. Neither The Ordinary nor DECIEM is affiliated with these trademarks or their owners.) Note: The name of this formula was inspired by some brands referring to
Numerous significant hits to DNA polymerase I sequences; e.g. residues 3-284 are 38% similar to (X98575) DNA-dependent DNA polymerase of Anaerocellum thermophilum; residues 3-286 are 34% similar to (U78771) DNA polymerase I (PolI) of Lactococcus lactis; and residues 2-285 are 32% similar to DP01_STRPN ...
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General Information: Bifidobacterium animalis subsp. lactis (strain DSM 10140 / JCM 10602 / LMG 18314) is an anaerobic Gram-positive lactic acid bacterium commonly found in the guts of healthy humans and has been identified in the infant gut biota, particularly in ileal, fecal, and mucosal samples. Some strains of B. animalis subsp. lactis are able to survive in the GIT, to adhere to human epithelial cells in vitro, to modify fecal flora, to modulate the host immune response, or to prevent microbial gastroenteritis and colitis. ...
The bio-purification of plant protein consortium led by NIZO, will be aimed to develop strategies to remove off-flavours of plant ingredients.
semasa abid berusia 2 minggu, kami membawanya ke ppukm untuk 1st appointment di klinik paediatrik (kardiologi)..kali ni kami jumpa dengan doktor bilqis (pakar jantung kanak-kanak)..di awal pertemuan doktor memberitahu ohh inilah parent abid ye..doktor bilqis ni sangat tegas orangnya tapi sangat baik..soalan dia kena kita jawab straight tak boleh bunga-bunga konar sana konar sini..kecut perut sokmo kalu nok jawab soalan dia huhu...dari hasil pemeriksaan terhadap abid, doktor bilqis sahkan abid mempunyai 5 masalah pada jantungnya..terduduk seketika sebaik saja doktor bilqis explain kepada kami..sebelum ni masa dalam kandungan kami diberitahu yang abid hanya mengalami masalah vsd sahaja..doktor bilqis terangkan yang dalam kandungan kita tak dapat nak lihat 100% kerana baby dalam kandungan kita tak boleh lihat dengan jelas keseluruhannya..dari perbincangan tu doktor bagitau plan rawatan untuk abid..memang tak da jalan lain kecuali pembedahan dan sejujurnya kami memang dah bersedia mental fizikal ...
Non-viable lactic acid bacteria (LAB) have been proposed as antigen delivery platforms called bacterium-like particles (BLPs). Most studies have been performed with Lactococcus lactis-derived BLPs where multiple antigens were attached to the peptidoglycan surface and used to successfully induce specific immune responses. It is well-established that the immunomodulatory properties of LAB are strain...
The exopolysaccharides of some strains of lactic acid bacteria, e.g., Lactococcus lactis subsp. cremoris, contribute a ...
Lactococcus lactis was proposed as the State Microbe because of its crucial contribution to the cheese industry in Wisconsin. ... The proposed AB 556 simply stated that Lactococcus lactis is the State Microbe and should be included in the Wisconsin Blue ... "2009 Assembly Bill 556" (PDF). "2017 Wisconsin Dairy Data" (PDF). "Lactococcus lactis Wisconsin State Microbe". advanced.bact. ... Lactococcus lactis is vital for manufacturing cheeses such as Cheddar, Colby, cottage cheese, cream cheese, Camembert, ...
Steidler, Lothar; Rottiers, Pieter (2006-08-01). "Therapeutic Drug Delivery by Genetically Modified Lactococcus lactis". Annals ... Lactococcus lactis and Bacteroides ovatus. The usage of recombinant microbes (i.e. microorganisms designed to contain DNA from ... The L. lactis mechanism of microbial drug delivery described in the 2006 study of Braat et al. uses a form of recombinant L. ... L. lactis is considered by the Food and Drug Administration (FDA) to be generally recognized as safe (GRAS), as it is commonly ...
... an abortive infection mechanism from Lactococcus lactis". Applied and Environmental Microbiology. 64 (12): 4748-56. doi:10.1128 ...
Lactococcus lactis, and Leuconostoc species. Buttermilk contains either Lactococcus lactis or L. bulgaricus. Other acidic ... Lactococcus lactis LA 103 and Streptococcus thermophillus LA 104 and reducing intestinal discomfort pursuant to Article 13(5) ... Pao cai contains L. pentosus, L. plantarum , Leuconostoc mesenteroides , L. brevis, L. lactis, and L. fermentum. A list of many ...
Beasley SS, Saris PE (August 2004). "Nisin-producing Lactococcus lactis strains isolated from human milk". Applied and ... and Lactococcus species, and later composed primarily of Veillonella, Prevotella, Leptotrichia, Lactobacillus, Streptococcus, ...
"Recombinant expression of Laceyella sacchari thermitase in Lactococcus lactis". Protein Expression and Purification. 92 (2): ...
Zevaco C, Monnet V, Gripon JC (1990). "Intracellular X-prolyl dipeptidyl peptidase from Lactococcus lactis spp. lactis: ... "Identification of the active site serine of the X-prolyl dipeptidyl aminopeptidase from Lactococcus lactis". FEBS Letters. 314 ... Ile The intracellular enzyme from Lactococcus lactis (190-kDa) is the type example of peptidase family S15. ... lactis DSM7290". Applied Microbiology and Biotechnology. 40 (1): 82-9. doi:10.1007/bf00170433. PMID 7765315. Habibi-Najafi MB, ...
Streptococcus lactis has been renamed to Lactococcus lactis subsp. lactis Food portal List of dairy products List of yogurt- ... "Transfer of Streptococcus lactis and related streptococci to the. genus Lactococcus gen. nov". Syst. Appl. Microbiol. 6 (2): ... Lactococcus, and Leuconostoc. The fermentation process increases the shelf life of the product while enhancing its taste and ...
A bacterial culture that produces lactic acid (Lactococcus lactis ssp. lactis or L. lactis ssp. cremoris strains such as are ... a small amount of low CO 2-producing citrate-fermenting lactococci or leuconostoc bacterial strains are added to the starter ...
Jørgensen CM, Hammer K, Martinussen J (2003). "CTP limitation increases expression of CTP synthase in Lactococcus lactis". J ...
"Secretion of Streptomyces tendae antifungal protein 1 by Lactococcus lactis". Brazilian Journal of Medical and Biological ...
"Lactococcus lactis YfiA is necessary and sufficient for ribosome dimerization". Molecular Microbiology. 91 (2): 394-407. doi: ... Well-known coding regions that do not have AUG initiation codons are those of lacI (GUG) and lacA (UUG) in the E. coli lac ...
bulgaricus, Lactobacillus helveticus, Lactobacillus kefiranofaciens, Lactococcus lactis, and Leuconostoc species. Lactobacilli ... Kluyveromyces lactis, and Saccharomyces fragilis, as well as strains of yeast that do not metabolize lactose, including ...
"Characterization of a Lactococcus lactis promoter for heterologous protein production". Biotechnology Reports. 17: 86-92. doi: ...
A hypothetical protein from the pepX region of Lactococcus lactis. MIP family channels consist of homotetramers (e.g., GlpF of ...
The Putative Lactococcus lactis Holin (LLHol) Family (TC# 1.E.44) consists of just a few proteins from Lactococcus lactis ... The Putative Lactococcus lactis Holin (LLHol) Family", which is licensed in a way that permits reuse under the Creative Commons ... "1.E.44 The Putative Lactococcus lactis Holin (LLHol) Family". TCDB. Retrieved 2016-03-29. Biology portal As of this edit, this ...
These genes possibly originated from Lactococcus lactis and S. gallolyticus, respectively. Another instance of lateral gene ...
bulgaricus (Lactobacillus bulgaricus), Lactobacillus helveticus, Lactococcus lactis, and Streptococcus salivarius subsp. ... homofermentative bacteria like Lactobacillus casei and Lactococcus lactis, producing two moles of lactate from one mole of ... The name reflects the lact- combining form derived from the Latin word lac, which means milk. In 1808, Jöns Jacob Berzelius ...
The Lactococcus lactis Phage r1t Holin (r1t Holin) Family (TC# 1.E.18) is a family of putative pore-forming proteins that ... The Lactococcus lactis phage r1t genome includes two adjacent genes, orf48 and orf49, which encode Orf48 (TC# 1.E.18.1.1; 75 ... Biology portal As of this edit, this article uses content from "1.E.18 The Lactococcus lactis Phage r1t Holin (r1t Holin) ... "1.E.18 The Lactococcus lactis Phage r1t Holin (r1t Holin) Family". Transporter Classification Database. Retrieved 2016-03-28. ...
The problem of choosing the correct acceleration of dilution has been studied with Escherichia coli and Lactococcus lactis ... "Multi-omics approach to study the growth efficiency and amino acid metabolism in Lactococcus lactis at various specific growth ... "Steady state growth space study of Lactococcus lactis in D-stat cultures". Antonie van Leeuwenhoek. 96 (4): 487-96. doi:10.1007 ...
"Cell surface of Lactococcus lactis is covered by a protective polysaccharide pellicle". Journal of Biological Chemistry. 285 ( ...
... and nucleotide sequence of the tagatose 6-phosphate pathway gene cluster of the lactose operon of Lactococcus lactis". J. Biol ... "Characterization of the lactose-specific enzymes of the phosphotransferase system in Lactococcus lactis". J. Biol. Chem. 265 ( ...
Visser S, Robben AJ, Slangen CJ (1991). "Specificity of a cell-envelope-located proteinase (PIII-type) from Lactococcus lactis ... "Diversity of cell envelope proteinase specificity among strains of Lactococcus lactis and its relationship to charge ... to hydrolyse hemoglobin and oxidized insulin B chain This enzyme is associated with the cell envelope of Lactococcus lactis and ...
2001). "The complete genome sequence of the lactic acid bacterium Lactococcus lactis ssp. lactis IL1403". Genome Res. 11 (5): ...
"Use of Lactococcus lactis to enrich sourdough bread with g-aminobutyric acid" (PDF). International Journal of Food Sciences and ...
It is made by fermenting whole milk with the bacterial culture Lactococcus lactis. When producing fermented milk products such ...
Nørager S, Arent S, Björnberg O, Ottosen M, Lo Leggio L, Jensen KF, Larsen S (August 2003). "Lactococcus lactis dihydroorotate ... Rowland P, Björnberg O, Nielsen FS, Jensen KF, Larsen S (June 1998). "The crystal structure of Lactococcus lactis ... "Active site of dihydroorotate dehydrogenase A from Lactococcus lactis investigated by chemical modification and mutagenesis". ...
... and functional characterization of secondary amino acid transporters of Lactococcus lactis". Journal of Bacteriology 195 (2): ...
Siia rühma kuulub näiteks juustu valmistamiseks kasutatavas piimhappebakteris Lactococcus lactis leiduv ja juustu valmimist ...
X-gal plates are differential for lac operon mutants.. Transport media[edit]. Transport media should fulfill these criteria: * ...
Lactococcus lactis, and Leuconostoc species.[32][33] Buttermilk contains either Lactococcus lactis or L. bulgaricus. ... Lactococcus lactis LA 103 and Streptococcus thermophillus LA 104 and reducing intestinal discomfort pursuant to Article 13(5) ... lactis, and L. fermentum. A list of many other bacteria found in several Asian fermented fruits and vegetables is also ...
Soured milk obtained by fermentation with mesophilic bacteria, mainly Lactococcus lactis and other bacterial cultures and ... and possible addition of the bacteria Streptococcus lactis and Leuconostoc citrovorum. Milk can be broken down into several ...
Lactococcus lactis. *Legionella adelaidensis. *Legionella cardiaca. *Lisozim. M. *Microcystis. *Microcystis aeruginosa. * ...
The LAB that are known for producing the aromas in sour cream are Lactococcus lactis ssp. lactis biovar diacetyllactis. ... that are utilized to allow the fermentation of sour cream production are Lactococcus lactis subsp latic or Lactococcus lactis ...
Lactococcus lactis bacterium dairy buttermilk [2] Lactococcus lactis bacterium chocolate [1] Lactococcus lactis ssp. cremoris ... Lactococcus lactis ssp. lactis bacterium cheese [5][6] Lactococcus raffinolactis bacterium cheese [2] ...
... protein sequence homology and has overlapping substrate specificities with the MDR-ABC transporter LmrA from Lactococcus lactis ...
Quark is possibly described by Tacitus in his book Germania as lac concretum ("thick milk"), eaten by Germanic peoples.[1][2] ... The lactic acid bacteria are introduced in the form of mesophilic Lactococcus starter cultures.[3][25][26] In the dairy ...
Lactobacillus bulgaricus dan Streptococcus lactis Beberapa spesies bakteri pengurai dan patogen dapat tumbuh di dalam makanan.[ ... Lactococcus, Streptococcus). Clostridia. Clostridiales (Clostridium, Peptostreptococcus, Selenomonas) · Halanaerobiales · ...
thermophilus (Streptococcus thermophilus) and Lactococcus lactis.. As a starting material for industrial production of lactic ... homofermentative bacteria like Lactobacillus casei and Lactococcus lactis, producing two moles of lactate from one mole of ...
thermophilus (Streptococcus thermophilus) dan Lactococcus lactis. Hampir sebarang sumber karbohidrat sumber yang mengandungi ... bakteri homofermentatif seperti Lactobacillus casei dan Lactococcus lactis yang menghasilkan dua mol laktat dari satu mol ... nama asid ini berasal daripada lac, perkataan Latin bagi susu. Pada 1808, Jöns Jacob Berzelius mendapati bahawa asid laktik ...
Lactococcus lactis) சுப்ச்ப். (subsp.) க்றேமொரிஸ் (cremoris) மற்றும் அசெடோபக்ட்டர் ஒரிண்டலிஸ் (Acetobacter orientalis) வகை ...
Another milk with Lactococcus lactis bacteria cultures ("cultured buttermilk") often is used in cooking to replace the ... which also kills the naturally occurring Lactococcus bacteria.[153] ...
The exopolysaccharides of some strains of lactic acid bacteria, e.g., Lactococcus lactis subsp. cremoris, contribute a ...
Smalla, Pamela LC; Watermanb, Scott R (June 1998). "Acid stress, anaerobiosis and gadCB: lessons from Lactococcus lactis and ...
... u pogledu proteinskih sekvenci i ima srodnu supstratnu specifičnost sa MDR-ABC transporterom LmrA iz Lactococcus lactis.[71] ...
Lactococcus species L. cremoris, L. lactis, and L. lactis biovar diacetylactis. These bacteria give it the taste that ...
Lactococcus dan lain-lain[sunting , sunting sumber]. Lactococcus lactis subsp. lactis, Lactococcus lactis subsp. cremoris, ... Starter kombinasi yang dapat digunakan terdiri atas lactobacillus acidophillus, l bulgaricus, l lactis, bifidobacterium longum ... Kluyveromyces lactis, Kluyveromyces marxianus, dan Pichia fermentans. ...
... is a Gram-positive bacterium used extensively in the production of buttermilk and cheese ... L. lactis subsp. lactis (formerly Streptococcus lactis)[23] is used in the early stages for the production of many cheeses, ... L. lactis is of crucial importance for manufacturing dairy products, such as buttermilk and cheeses. When L. lactis ssp. lactis ... Lactococcus lactis uses. *^ Kok, J., Buist, G., Zomer, A. L., van Hijum, S. a F. T., & Kuipers, O. P. (2005). "Comparative and ...
Shearman C, Godon JJ, Gasson M (July 1996). "Splicing of a group II intron in a functional transfer gene of Lactococcus lactis ... "Splicing of a group II intron involved in the conjugative transfer of pRS01 in lactococci". J Bacteriol. 178 (12): 3531-8. doi: ...
... and Lactococcus lactis. Some experts say that there is evidence that probiotic strains reduce diarrhea, irritable bowel ... While indicating Bifidobacterium lactis, the package does not list that Activia also contains strains commonly found in Yogurt ... Bifidus Digestivum and Bifidobacterium Lactis. Danone launched Activia in France in 1987 under the "Bio" brand name. The claims ... Bifidus Digestivum and Bifidobacterium Lactis. 1987: France 1988: Belgium, Spain, and the United Kingdom 1989: Italy 2002: ...
For example, when grown in the presence of both glucose and maltose, Lactococcus lactis will produce enzymes to metabolize ... Monod later put aside his work on diauxic growth and focused on the lac operon model of gene expression, which led to a Nobel ... During his work on the lac operon of E. coli, Joshua Lederberg isolated β-galactosidase and found it in greater quantities in ... Thus, if glucose is present, cAMP levels remain low, so CAP is unable to activate transcription of the lac operon, regardless ...
... and then inoculated with a culture of Lactococcus lactis or Lactobacillus bulgaricus plus Leuconostoc citrovorum to simulate ...
... and may include Lactococcus lactis, Streptococcus diacetylactis, Streptococcus cremoris, Lactobacillus delbrueckii subsp. ...
Lactococcus_lactis Lactococcus lactis uses Kok J, Buist G, Zomer AL, van Hijum SA, Kuipers OP (2005). "Comparative and ... Nakamura S, Morimoto YV, Kudo S (2015). "A lactose fermentation product produced by Lactococcus lactis subsp. lactis, acetate, ... L. lactis is of crucial importance for manufacturing dairy products, such as buttermilk and cheeses. When L. lactis ssp. lactis ... L. lactis subsp. lactis (formerly Streptococcus lactis) is used in the early stages for the production of many cheeses, ...
... origin of replication and modular structure of the Lactococcus lactis lytic bacteriophage sk1". Molecular Microbiology 26 (1): ...
Lactococcus lactis, Streptomyces coelicolor, Aspergillus oryzae, Aspergillus niger, Penicilium chrysogenum and Pichia pastoris ...
Lactococcus_lactis Lactococcus lactis uses Kok J, Buist G, Zomer AL, van Hijum SA, Kuipers OP (2005). "Comparative and ... Nakamura S, Morimoto YV, Kudo S (2015). "A lactose fermentation product produced by Lactococcus lactis subsp. lactis, acetate, ... L. lactis is of crucial importance for manufacturing dairy products, such as buttermilk and cheeses. When L. lactis ssp. lactis ... L. lactis subsp. lactis (formerly Streptococcus lactis) is used in the early stages for the production of many cheeses, ...
Complete Genome Sequence of Lactococcus lactis subsp. lactis KF147, a Plant-Associated Lactic Acid Bacterium Roland J. Siezen, ... The MG1363 and IL1403 Laboratory Strains of Lactococcus lactis and Several Dairy Strains Are Diploid Ole Michelsen, Flemming G ... Genome Sequences of Lactococcus lactis MG1363 (Revised) and NZ9000 and Comparative Physiological Studies Daniel M. Linares, Jan ... Crystal Structure of ORF12 from Lactococcus lactis Phage p2 Identifies a Tape Measure Protein Chaperone Marina Siponen, ...
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Lactococcus lactis. Lactococcus lactis is a Gram-positive bacterium used extensively in the production of buttermilk and cheese ... L. lactis subsp. lactis (formerly Streptococcus lactis)[23] is used in the early stages for the production of many cheeses, ... L. lactis is of crucial importance for manufacturing dairy products, such as buttermilk and cheeses. When L. lactis ssp. lactis ... Lactococcus lactis uses. *^ Kok, J., Buist, G., Zomer, A. L., van Hijum, S. a F. T., & Kuipers, O. P. (2005). "Comparative and ...
Heterologous gene expression of bovine plasmin in Lactococcus lactis.. Arnau J1, Hjerl-Hansen E, Israelsen H. ... Heterologous production of bovine plasmin was studied in the industrially relevant bacterium Lactococcus lactis. Two sets of ...
lactis Il1403. Mutation(s): 1 Gene Names: zitR, L168265. Find proteins for Q9CDU5 (Lactococcus lactis subsp. lactis (strain ...
This topic has 4 study abstracts on Lactococcus lactis indicating that they may have therapeutic value in the treatment of ... Lactococcus lactis KA-FF 1-4 reduces vancomycin-resistant enterococci and impacts the human gut microbiome. Jun 30, 2020. ... Milk fermented with Lactococcus lactis ssp. cremoris JFR1 is effective in vitro in the reduction of Salmonella invasion into ... 14 Abstracts with Lactococcus lactis Research. Filter by Study Type. Animal Study. ...
lactis ATCC ® 19435D-5™ Designation: Genomic DNA from Lactococcus lactis subsp. lactis strain NCTC 6681 TypeStrain=True ... Lactococcus lactis subsp. lactis (Lister) Schleifer et al. (ATCC® 19435D-5™) Click here to learn about our Enhanced ... Lactococcus lactis subsp. lactis (Lister) Schleifer et al. (ATCC® 19435™) Add to ... Strain Designations: Genomic DNA from Lactococcus lactis subsp. lactis strain NCTC 6681 [ATCC® 19435™] / Type Strain: yes / ...
Protein balance of Lactococcus lactis in cheese production. Status: Completed Start project:. Oct 1, 2016. End project:. Oct 1 ... Protein balance of Lactococcus lactis in cheese production Lactic acid bacteria are used as starter cultures in dairy ... The overall aim is to gain knowledge on the trade-offs occurring in Lactococcus lactis under industrially relevant conditions ... In this project, we investigate, by proteomics, regulation of protein synthesis and turnover in Lactococcus lactis under ...
"Cloning and transcriptional analysis of two threonine biosynthetic genes from Lactococcus lactis MG1614.". Madsen S.M., ... sp,P52985,DHOM_LACLC Homoserine dehydrogenase OS=Lactococcus lactis subsp. cremoris OX=1359 GN=hom PE=3 SV=1 ... Lactococcus lactis subsp. cremoris (Streptococcus cremoris). ,p>This subsection of the ,a href="http://www.uniprot.org/help/ ... cellular organisms › Bacteria › Terrabacteria group › Firmicutes › Bacilli › Lactobacillales › Streptococcaceae › Lactococcus ...
Standardized assay medium to measure Lactococcus lactis enzyme activities while mimicking intracellular conditions.. Goel A1, ... Current kinetic parameters for Lactococcus lactis are scattered through different studies and performed under different assay ... Standardized Assay Medium To Measure Lactococcus lactis Enzyme Activities while Mimicking Intracellular Conditions ... Standardized Assay Medium To Measure Lactococcus lactis Enzyme Activities while Mimicking Intracellular Conditions ...
It was shown that some Lactococcus strains possess two galactose-specific systems - a permease and a PTS, even if they lack the ... It was shown that some Lactococcus strains possess two galactose-specific systems - a permease and a PTS, even if they lack the ... galactose metabolism in Lactococcus lactis has been in debate. Different studies led to diverse outcomes making it difficult to ... galactose metabolism in Lactococcus lactis has been in debate. Different studies led to diverse outcomes making it difficult to ...
Treatment of Murine Colitis by Lactococcus lactis Secreting Interleukin-10. By Lothar Steidler, Wolfgang Hans, Lieven Schotte, ... Treatment of Murine Colitis by Lactococcus lactis Secreting Interleukin-10. By Lothar Steidler, Wolfgang Hans, Lieven Schotte, ... Treatment of Murine Colitis by Lactococcus lactis Secreting Interleukin-10 Message Subject. (Your Name) has forwarded a page to ... Intragastric administration of IL-10-secretingLactococcus lactis caused a 50% reduction in colitis in mice treated with dextran ...
Biotin metabolism - Lactococcus lactis subsp. lactis Il1403 [ Pathway menu , Organism menu , Pathway entry , Download KGML , ...
... and efficiency of nisin-induced expression directed by the nisA promoter region were studied in Lactococcus lactis with ... Expression of the gusA gene was also studied in L. lactis NZ9800, an NZ9700 derivative carrying a deletion in the structural ... In the nisin-producing L. lactis strain NZ9700, the specific b-glucuronidase activity increased very rapidly after mid- ... nisA gene that abolishes nisin production, and in L. lactis NZ3900, an MG1363 derivative containing the regulatory nisRK genes ...
"Lactococcus lactis" by people in Harvard Catalyst Profiles by year, and whether "Lactococcus lactis" was a major or minor topic ... "Lactococcus lactis" is a descriptor in the National Library of Medicines controlled vocabulary thesaurus, MeSH (Medical ... Lactococcus lactis*Lactococcus lactis. *Streptococcus lactis. Lactococcus lactis subsp. lactis*Lactococcus lactis subsp. lactis ... New insights into the complex regulation of the glycolytic pathway in Lactococcus lactis. II. Inference of the precisely timed ...
In this work we tested the usefulness of Lactococcus lactis, the most extensively studied lactic acid bacterium, as a delivery ... lactis harboring the surface-exposed rCjaAD antigen afforded greater protection than the L. lactis producing cytoplasm-located ... Next, rCjaAD and CjaA were fused to the C-terminus of the L. lactis YndF containing the LPTXG motif. The genes expressing these ... Next, rCjaAD and CjaA were fused to the C-terminus of the L. lactis YndF containing the LPTXG motif. The genes expressing these ...
Terpenoid backbone biosynthesis - Lactococcus lactis subsp. cremoris A76 [ Pathway menu , Organism menu , Pathway entry , ...
... showing a Lactococcus lactis bacterium. It belongs to the Streptococcus genus and is usually arranged in chains formed by ... Lactococcus lactis is one of the bacteria involved in the production of lactic acid during milk fermentation. Lactic acid is a ... Lactococcus lactis has a diameter of approximately 1 micrometer; the green areas within the bacterium show regions where the ... Coloured transmission electron micrograph (TEM) showing a Lactococcus lactis bacterium. It belongs to the Streptococcus genus ...
Lactococcus lactis was isolated from a culture of th ... Lactococcus lactis was isolated from a culture of the abscess ... Lactococcus lactis / isolation & purification*. Liver Abscess / microbiology*, therapy, ultrasonography. Male. Microbial ... This is the first published description of portal vein thrombosis and pyogenic liver abscess due to L. lactis.. ...
Lactobacillus plantarum, Lactobacillus rhamnosus, Lactobacillus salivarius, Lactococcus lactis, Streptococcus thermophilus. ... Tags: Bifidobacterium lactis, Bifidobacterium longum, Lactbaacillus acidophilus, Lactobacillus brevis, Lactobacillus bulgaricus ... Bifidobacterium lactis, Bifidobacterium longum, Lactbaacillus acidophilus, Lactobacillus brevis, Lactobacillus bulgaricus, ...
Draft Genome Sequences of Three Amino Acid-Secreting Lactococcus lactis Strains. Hernandez-Valdes, J. A., de Jong, A., Kok, J. ... Draft Genome Sequences of Three Amino Acid-Secreting Lactococcus lactis Strains. / Hernandez-Valdes, Jhonatan A.; de Jong, Anne ... N2 - Three Lactococcus lactis strains with the ability to secrete various amino acids (leucine, isoleucine, methionine, valine ... AB - Three Lactococcus lactis strains with the ability to secrete various amino acids (leucine, isoleucine, methionine, valine ...
Lactococcus lactis is a probiotic bacterium that produces various bacteriocins. Periodontopathogens induce inflammation and ... To investigate the antimicrobial peptide of L. lactis, the spent culture medium (SCM) of L. lactis was treated with or without ... To evaluate the neutralizing effect of L. lactis on halitosis, SCM of periodontopathogens was mixed with an L. lactis ... lactis inhibited the production of IL-6 and TNF-α induced by the LPS. These results suggest that L. lactis may be a useful ...
... ... J. E. Garneau, D. M. Tremblay, and S. Moineau, "Characterization of 1706, a virulent phage from Lactococcus lactis with ...
... Osmoregulated ABC-transport system of Lactococcus lactis senses water stress via changes in the physical state of the membrane ... Osmoregulated ABC-transport system of Lactococcus lactis senses water stress via changes in the physical state of the membrane ... Osmoregulated ABC-transport system of Lactococcus lactis senses water stress via changes in the physical state of the membrane ...
... and lactic acid and bacteriocin production byLactococcus lactis subsp.lactis 140 NWC was studied during batch fermentation i ... Influence of pH on growth and bacteriocin production byLactococcus lactis subsp.lactis 14ONWC during batch fermentation. *E. ... The influence of pH on growth, and lactic acid and bacteriocin production byLactococcus lactis subsp.lactis 140 NWC was studied ... De Vuyst L, Vandamme EJ (1992) Influence of the carbon source on nisin production inLactococcus lactis subsplactis batch ...
Oral Vaccination with the Porcine Rotavirus VP4 Outer Capsid Protein Expressed by Lactococcus lactis Induces Specific Antibody ... "Oral Vaccination with the Porcine Rotavirus VP4 Outer Capsid Protein Expressed by Lactococcus lactis Induces Specific Antibody ...
lactis LABW4 was isolated from naturally fermented milk product which exhibited strong antibacterial activity against Listeria ... A bacteriocin producing strain of Lactococcus lactis subsp. ... Use of Bacteriocin Producing Lactococcus lactis subsp. lactis ... Barman, S. , Ghosh, R. and Mandal, N. (2014) Use of Bacteriocin Producing Lactococcus lactis subsp. lactis LABW4 to Prevent ... A bacteriocin producing strain of Lactococcus lactis subsp. lactis LABW4 was isolated from naturally fermented milk product ...
  • L. lactis subsp. (wikipedia.org)
  • Complete Genome Sequence of Lactococcus lactis subsp. (asm.org)
  • Lactococcus lactis subsp. (uniprot.org)
  • Fermented camel milk by lactococcus lactis subsp creemoris could reduce carbon tetrachloride induced heart oxidative damages. (greenmedinfo.com)
  • A17, the first sequenced strain of Lactococcus lactis subsp. (greenmedinfo.com)
  • Growth space of Lactococcus lactis subsp. (springer.com)
  • Even S, Lindley ND, Cocaign-Bousquet M (2003) Transcriptional, translational and metabolic regulation of glycolysis in Lactococcus lactis subsp. (springer.com)
  • Genomic DNA from Lactococcus lactis subsp. (atcc.org)
  • DHOM_LACLC Homoserine dehydrogenase OS=Lactococcus lactis subsp. (uniprot.org)
  • To establish uniform assay conditions that resemble intracellular conditions, we analyzed the intracellular composition of anaerobic glucose-limited chemostat cultures of L. lactis subsp. (nih.gov)
  • Microencapsulation of probiotics in hydrogel particles: enhancing Lactococcus lactis subsp. (harvard.edu)
  • Terpenoid backbone biosynthesis - Lactococcus lactis subsp. (genome.jp)
  • Influence of pH on growth and bacteriocin production by Lactococcus lactis subsp. (springer.com)
  • De Vuyst L, Vandamme EJ (1992) Influence of the carbon source on nisin production in Lactococcus lactis subsp lactis batch fermentations. (springer.com)
  • Use of Bacteriocin Producing Lactococcus lactis subsp. (scirp.org)
  • We report the first description, confirmed by bacteriologic and molecular (polymerase chain reaction and pulsed-field gel electrophoresis) analysis, of an infection in animals caused by Lactococcus lactis subsp. (cdc.gov)
  • In humans, L. garvieae and L. lactis subsp. (cdc.gov)
  • However, infection by L. lactis subsp. (cdc.gov)
  • We present the first microbiologic and molecular evidence for infection produced by L. lactis subsp. (cdc.gov)
  • All 11 clinical isolates had an identical biochemical profile, which was identified as L. lactis subsp. (cdc.gov)
  • Specific primers LLF 5'-GCAATTGCATCACTCAAAGA and LLR 5'-ACAGAGAACTTATAGCTCCC were designed from diagnostic regions of the L. lactis subsp. (cdc.gov)
  • 4GC3: Crystal structure of L-HISTIDINOL PHOSPHATE PHOSPHATASE (HISK) from Lactococcus lactis subsp. (rcsb.org)
  • Six Putative Two-Component Regulatory Systems Isolated From Lactococcus Lactis Subsp. (nih.gov)
  • Large plasmidome of dairy Lactococcus lactis subsp. (biomedcentral.com)
  • The dairy isolate L. lactis subsp. (biomedcentral.com)
  • In this study, the complete nucleotide sequences of twelve plasmids of the dairy isolate of L. lactis subsp. (biomedcentral.com)
  • It is produced by lactic acid bacteria Lactococcus lactis subsp. (elsevier.com)
  • Characterization of a bacteriocin produced by Lactococcus lactis subsp. (semanticscholar.org)
  • Nisin Z Production by Lactococcus lactis subsp. (semanticscholar.org)
  • 7] studies on use of a genetically engineered Lactococcus lactis subsp. (scirp.org)
  • Characterization of the structural gene encoding nisin F, a new lantibiotic produced by a Lactococcus lactis subsp. (sun.ac.za)
  • paracasei ST11BR, Lactobacillus plantarum ST13BR, Lactobacillus pentosus ST151BR and Lactococcus lactis subsp. (sun.ac.za)
  • The lantibiotic nisin is produced by several strains of Lactococcus lactis subsp. (microbiologyresearch.org)
  • Eighty-eight contigs larger than 100 bp were mapped to the published genome of L. lactis subsp. (asm.org)
  • The genome sequence and annotation of Lactococcus lactis subsp. (asm.org)
  • Cloning and expression of a novel lactococcal aggregation factor from Lactococcus lactis subsp. (biomedcentral.com)
  • This 136 kDa surface-bound protein, encoded by the chromosomally located sex factor of Lactococcus lactis subsp. (biomedcentral.com)
  • Furthermore, the aggregation ability of L. lactis subsp. (biomedcentral.com)
  • We report here the genome sequence of IL6288, a prophage-free derivative of Lactococcus lactis subsp. (inrae.fr)
  • Antimicrobial interaction of Lactococcus lactis subsp. (journaldatabase.info)
  • A research was conducted to determine the interaction of antimicrobial compound produced by Lactococcus lactis subsp. (journaldatabase.info)
  • Salmonella exhibited the highest inhibition zone of 20.00±1.23mm at selected Lactococcus lactis subsp. (journaldatabase.info)
  • Staphylococcus aureus showed the highest inhibition zone of 20.00±2.13mm at initial substrate pH 7.0, with Lactococcus lactis subsp. (journaldatabase.info)
  • The motilities of Pseudomonas, Vibrio, and Leptospira strains were also severely disrupted by lactose utilization by L. lactis. (wikipedia.org)
  • It was shown that some Lactococcus strains possess two galactose-specific systems - a permease and a PTS, even if they lack the lactose utilization plasmid, proving that a lactose-independent PTS Gal exists. (frontiersin.org)
  • Bacteriophages of Streptococcus thermophilus and Lactococcus lactis , two important starter strains in the dairy industry ( 5 , 25 ), share significant sequence homology ( 3 , 6 ). (asm.org)
  • For transduction assays with plasmid-free strains S. thermophilus a10 and L. lactis Bu2-60 ( 20 ), cultures grown in thM17 (strain a10) or in GM17 ( 28 ) (strain Bu2-60) were harvested in the logarithmic phase (OD 620 , 0.5) and resuspended in 0.2 volume of 10 mM ice-cold MgSO 4 (approximately 1 × 10 9 CFU/ml). (asm.org)
  • abstract = "Three Lactococcus lactis strains with the ability to secrete various amino acids (leucine, isoleucine, methionine, valine, glutamic acid, and histidine) were sequenced in order to identify the mechanisms involved in the secretion. (rug.nl)
  • Study of gene transfer in vitro and in the digestive tract of gnotobiotic mice from Lactococcus lactis strains to various strains belonging to human intestinal flora. (springer.com)
  • The autolysis and proteolysis are important features in the strains of L. lactis used in the manufacture of cheese. (scirp.org)
  • The objective of this work was to assess the autolytic and proteolytic activities of 21 strains of L. lactis iso lated from diverse sources. (scirp.org)
  • C. Nájera-Domínguez and N. Gutiérrez-Méndez, "Autolytic and Proteolytic Properties of Strains of Lactococcus lactis Isolated from Different Vegetables, Raw-Milk Cheeses and Commercial Starter Cultures," Food and Nutrition Sciences , Vol. 4 No. 11A, 2013, pp. 21-26. (scirp.org)
  • J. Ramírez-Nunez, R. Romero-Medrano, G. V. NevárezMoorillon and N. Gutiérrez-Méndez, "Effect of pH and Salt Gradient on the Autolysis of Lactococcus lactis Strains," Brazilian Journal of Microbiology, Vol. 42, No. 4, 2011, pp. 1495-1499. (scirp.org)
  • At the same time, it has been demonstrated that certain L. lactis strains (e.g. (thefreelibrary.com)
  • These have been included in the appendix (section A). The screening did not reveal any L. lactis strains capable of assimilating glycerol nor did it reveal any conditions favorable to glycerol dissimilation in L. lactis. (dtu.dk)
  • Cheddar cheese was made in duplicate using two strains of lactis and cremoris . (usu.edu)
  • lactis biovar diacetylactis FM03P was sequenced revealing the biggest plasmidome of all completely sequenced and published L. lactis strains up till now. (biomedcentral.com)
  • Thus the review can be a guide for the appropriate selection of strains and site for the expression system in L.lactis. (alliedacademies.org)
  • Monitoring transfer of recombinant and nonrecombinant plasmids between Lactococcus lactis strains and members of the human gastrointestinal microbiota in vivo--impact of donor cell number and diet. (semanticscholar.org)
  • Plasmid transfer between Lactococcus lactis strains and between donor strains and human gut bacteria was monitored. (semanticscholar.org)
  • METHODS AND RESULTS Transfer of the recombinant plasmid pCK1 and/or the promiscuous nonrecombinant plasmid pAMbeta1 between L. lactis strains was monitored in vivo in HBA rats. (semanticscholar.org)
  • Like many probiotic strains, Lactococcus lactis has been used for hundreds of years to ferment foods such as cheese, yoghurt, sauerkraut. (balanceone.com)
  • Because nisin cannot be synthesized chemically, Lactococcus lactis strains are commonly used for this purpose. (balanceone.com)
  • Because L. lactis can survive the harsh conditions of the gastrointestinal tract but doesn't colonize the gut (like other Lactobacillus strains), it can be used as a "vehicle" to deliver therapeutics such as cytokines into the human body. (balanceone.com)
  • Lactose plasmid conjugation in Lactococcus lactis 712 and in the related strains C2 and ML3, frequently involves plasmid co-integration with a sex factor. (biomedcentral.com)
  • It is used for cloning in Lactococcus strains and in strains of other lactic acid bacteria genera that do not have the regulatory genes integrated into the chromosome. (bocascientific.com)
  • The genome content and different phenotypes have been determined for multiple L. lactis strains in order to understand intra-species genotype and phenotype diversity and annotate gene functions. (ru.nl)
  • In this study, we identified relations between gene presence and a collection of 207 phenotypes across 38 L. lactis strains of dairy and plant origin. (ru.nl)
  • In addition to all gene-phenotype relations, we also provide coherent phenotype data for 38 Lactococcus strains assessed in 207 different phenotyping experiments, which to our knowledge is the largest to date for the Lactococcus lactis species. (ru.nl)
  • The unique lactic acid bacteria, Lactococcus lactis strain plasma (LC-Plasma), stimulates plasmacytoid dendritic cells, which play an important role in viral infection. (humankinetics.com)
  • Cheddar cheese is manufactured by fermenting milk with lactic acid bacteria added as Lactococcus lactis . (usu.edu)
  • Lactic acid bacteria (LAB) are gram positive bacteria that include Lactococci, streptococci , and lactobacilli, all of which have long been used as starters for food fermentations [ 1 ]. (alliedacademies.org)
  • Although Lactococcus lactis is related to other lactic acid bacteria (such as Lactobacillus acidophilus ) that colonize the intestines and mouth, L. lactis doesn't usually colonize human tissues . (balanceone.com)
  • 1997). ' Controlled gene expression systems for lactic acid bacteria: transferable nisin-inducible expression cassettes for Lactococcus, Leuconostoc, and Lactobacillus spp . (bocascientific.com)
  • in the genome of L. lactis MG1363. (wikipedia.org)
  • Jensen NBS, Melchiorsen CR, Jokumsen KV, Villadsen J (2001) Metabolic behavior of Lactococcus lactis MG1363 in microaerobic continuous cultivation at a low dilution rate. (springer.com)
  • A novel plant-niche-related PTS component Llmg_0963 forming a hybrid transporter Llmg_0963PtcBA and a glucose/mannose-specific PTS are shown to be involved in galactose transport in L. lactis MG1363. (frontiersin.org)
  • Expression of the gusA gene was also studied in L. lactis NZ9800, an NZ9700 derivative carrying a deletion in the structural nisA gene that abolishes nisin production, and in L. lactis NZ3900, an MG1363 derivative containing the regulatory nisRK genes integrated in the chromosome. (psu.edu)
  • The genetic elements specifying six putative two-component regulatory systems (2CSs) were identified on the chromosome of Lactococcus lactis MG1363. (nih.gov)
  • AcmA, the major autolysin of Lactococcus lactis MG1363 is a modular protein consisting of an N-terminal active site domain and a C-terminal peptidoglycan-binding domain. (nih.gov)
  • Results: This study describes a transcriptome-phenotype matching approach in which the starter L. lactis MG1363 was fermented under a variety of conditions that differed in the levels of oxygen and/or salt, as well as the fermentation pH and temperature. (uva.nl)
  • To validate this transcriptome-phenotype association we grew L. lactis MG1363 in the absence of cysteine which led to enhanced robustness towards oxidative stress. (uva.nl)
  • In the genome of the gram-positive bacterium Lactococcus lactis MG1363, we have identified three genes ( clpC , clpE , and clpB ) which encode Clp proteins containing two conserved ATP binding domains. (pubmedcentralcanada.ca)
  • Transfer of Streptococcus lactis and related streptococci to the genus Lactococcus gen. nov. (atcc.org)
  • M58837 Streptococcus lactis 16S ribosomal RNA. (atcc.org)
  • Using Streptococcus thermophilus phages, plasmid transduction in Lactococcus lactis was demonstrated. (asm.org)
  • In order to develop a new system for the analysis of capsular biosynthetic pathways we have explored the possibility of expressing type 3 capsular polysaccharide (CPS) from the pathogen Streptococcus pneumoniae in Lactococcus lactis , an unencapsulated lactic acid bacterium being developed as a vaccine delivery vehicle for mucosal immunization. (asm.org)
  • To establish this system we used as an initial model the expression in Lactococcus lactis of the capsule biosynthesis genes of Streptococcus pneumoniae type 3 (strain WU2). (asm.org)
  • Plasmid complements of Streptococcus lactis NCDO 712 and other lactic streptococci after protoplast-induced curing. (bocascientific.com)
  • Construction of a vector plasmid family and its use for molecular cloning in Streptococcus lactis. (bocascientific.com)
  • 1995). In the presence of both SSOs, no ss plasmid DNA was observed in L. lactis. (rti.org)
  • In the presence of both SSOs, the plasmid was stably maintained in L. lactis under nonselective growth conditions. (rti.org)
  • The pXYCYT:Hsp65 plasmid was transformed into the L. lactis NCDO2118 FnBPA+ strain, resulting in the L. lactis NCDO2118 FnBPA+ (pXYCYT:Hsp65) strain. (physiciansweekly.com)
  • The replicons of the vectors pNZ8008, pNZ8148, pNZ8149 and pNZ8150 are identical and came originally from the Lactococcus lactis plasmid pSH71. (mobitec.com)
  • A recombinant Lactococcus lactis strain NZ9000-401-kiss1 harboring a plasmid containing the gene of the tumor metastasis-inhibiting peptide KiSS1 was constructed. (biomedcentral.com)
  • Plasmids constitute the main toolbox for L. lactis genetic engineering and most rely on antibiotic resistant markers for plasmid selection and maintenance. (biomedcentral.com)
  • Lcn972 is a non-lantibiotic bacteriocin encoded by the 11-kbp plasmid pBL1 with a potent antimicrobial activity against Lactococcus . (biomedcentral.com)
  • The aggregation phenomenon in lactococci is directly associated with the sex factor and lactose plasmid co-integration event or duplication of the cell wall spanning (CWS) domain of PrtP proteinase. (biomedcentral.com)
  • The gene involved in aggregation ( aggL ) was mapped on plasmid pKP1 (16.2 kb), cloned and expressed in homologous and heterologous lactococci and enterococci. (biomedcentral.com)
  • The NICE pNZ9530 Lactococcus lactis nisRnisK vector , part of the NICE expression system, is a low copy plasmid with pAMΒ1 origin of replication that carries the nisR and nisK genes. (bocascientific.com)
  • Penelitian ini terdiri dari beberapa tahap yaitu analisis sekuen gen HBcAg yang tersisip di plasmid pNZ8148, optimasi waktu induksi terhadap L. lactis yang mengandung plasmid rekombinan pNZ8148-HBcAg, dan ekspresi gen HBcAg pada tingkat transkripsi dan tingkat translasi. (ipb.ac.id)
  • Hasil analisis sekuen gen HBcAg yang tersisip di dalam plasmid pNZ8148 dan telah diintroduksikan ke dalam bakteri L. lactis menunjukkan tidak terdapat mutasi dan 100% identik dengan sekuen HBcAg dari HBV subgenotip B3 (Gene bank nomor aksesi 1839 Java). (ipb.ac.id)
  • L. lactis is one of the best characterized low GC Gram positive bacteria with detailed knowledge on genetics, metabolism and biodiversity. (wikipedia.org)
  • Bacteriophages specific to L. lactis cause significant economic losses each year by preventing the bacteria from fully metabolizing the milk substrate. (wikipedia.org)
  • Lactose fermentation In Shuichi Nakamura's, Yusuke V. Marimoto, and Seishi Kudo's study, they sought to prove that some fermentation produced by L. lactis can hinder motility in pathogenic bacteria. (wikipedia.org)
  • A probiotic bacteria found within kimchi known as Lactococcus lactis produces a nisin-like bacteriocin with significant antimicrobial activity. (greenmedinfo.com)
  • Lactococcus lactis is one of the bacteria involved in the production of lactic acid during milk fermentation. (sciencephoto.com)
  • Phage lysates (150 μl) were added at an MOI of 0.01 ( S. thermophilus ) or 0.1 ( L. lactis ) to 300 μl of bacteria. (asm.org)
  • Lactococcus lactis are noninvasive, nonpathogenic bacteria, used widely in manufacturing of milk fermentation products. (thefreelibrary.com)
  • Additionally, the general inability of L. lactis as noncommensal, noncolonizing bacteria to grow or replicate in vivo limits its potential to evoke oral tolerance [8, 16]. (thefreelibrary.com)
  • By using the bacteria Salmonella Flagellar , researchers were able to use L. lactis to produce acetate (through lactose fermentation) and subsequently interrupt the rotation of the bacteria's flagella - thus hindering the ability of the Salmonella to move through the body. (balanceone.com)
  • Total levels of Immuno-globulin A and Immunoglobulin E, Lac+ and Lac- bacteria and Lactobacillus were measured. (proteinpeptideletters.com)
  • Results: The main results indicate that the association between resveratrol and probiotics was able to decrease mice body weight, as compared to the other groups, in addition to decrease the number of Lac- bacteria and increasing the number of Lac+ bacteria. (proteinpeptideletters.com)
  • Lactococcus lactis is a Gram-positive bacterium used extensively in the production of buttermilk and cheese, but has also become famous as the first genetically modified organism to be used alive for the treatment of human disease. (wikipedia.org)
  • The osmoregulated transporter for quaternary compounds (OpuA) in L. lactis constitutes the main system to protect the organism against hyperosmotic stress ( 2 , 3 ), and equivalent activities have been described for other organisms ( 1 , 4 ). (pnas.org)
  • lactis LABW4 was isolated from naturally fermented milk product which exhibited strong antibacterial activity against Listeria monocytogenes MTCC657, a food spoilage psychrophilic organism. (scirp.org)
  • Specifically, the goal was to adapt the model organism Lactococcus lactis to convert crude glycerol, to value-added fuels or chemicals. (dtu.dk)
  • This organism is easy to handle and, with improved understanding on the genetic level, the last couple of decades has resulted in the development of a number of gene expression systems based on L.lactis [ 2 , 3 ]. (alliedacademies.org)
  • Lactococcus lactis cremoris is commonly considered to be a non-pathogenic organism in humans. (biomedcentral.com)
  • Lactococcus lactis is a cocci, Gram-positive bacterium used extensively in the production of buttermilk and cheese as well as being the first genetically modified organism used live to treat human disease. (ximbio.com)
  • A specific characteristic of L. lactis is that cells are grouped in pairs and short chains and can appear ovoid in morphology, L. lactis is also a nonsporulating and non-motile organism. (ximbio.com)
  • Prenatal exposure to the probiotic Lactococcus lactis decreases anxiety-like behaviour. (greenmedinfo.com)
  • Lactococcus lactis is a probiotic bacterium that produces various bacteriocins. (go.jp)
  • These results suggest that L. lactis may be a useful probiotic to prevent and treat periodontitis and halitosis. (go.jp)
  • Lactococcus lactis (L. lactis) is an important probiotic in intestine of human and animal. (thefreelibrary.com)
  • Confocal fluorescence microscopy confirmed that Lactococcus lactis were trapped within calcium alginate beads formed by dripping a probiotic-alginate mixture into a calcium solution. (rsc.org)
  • Probiotic properties of Lactococcus lactis ssp. (sun.ac.za)
  • Objective: In this study, our motivation was to evaluate the effects of the association between Resveratrol and Lactococcus lactis, a probiotic, on the composition of the gastrointestinal microbiota and body weight of mice. (proteinpeptideletters.com)
  • Oral and Intra nasal vaccination with recombinant Lactococcus lactis can induce high level immune responses to tetanus toxin fragment C elicits. (springer.com)
  • For efficient oral delivery of sRANKL, we constructed recombinant Lactococcus lactis (L. lactis) IL1403 secreting sRANKL (sRANKL-LAB). (sigmaaldrich.com)
  • Recombinant Lactococcus lactis Expressing Haemagglutinin from a Polish Avian H5N1 Isolate and Its Immunological Effect in Preliminary Animal Trials. (thefreelibrary.com)
  • A recombinant Lactococcus lactis which produced and secreted pEGF at 1000 ng/ml in culture supernatant was generated. (biomedcentral.com)
  • We have generated a recombinant Lactococcus lactis which produced and secreted fully biologically active porcine EGF. (biomedcentral.com)
  • Since the 1970s, galactose metabolism in Lactococcus lactis has been in debate. (frontiersin.org)
  • Lahtvee PJ, Adamberg K, Arike L, Nahku R, Aller K, Vilu R. Multi-omics approach to study the growth efficiency and amino acid metabolism in Lactococcus lactis at various specific growth rates. (harvard.edu)
  • for glycerol utilization, engineering of glycerol metabolism in L. lactis and finally an investigation into perturbation of energy metabolism in L. lactis. (dtu.dk)
  • The engineering of glycerol metabolism in L. lactis was initiated from three different perspectives: overexpression of glycerol kinase from L. lactis, introduction of a heterologous glycerol assimilation pathway and construction of a library of NADH oxidase activity. (dtu.dk)
  • Based on the acquired knowledge on its physiology and metabolism, new applications have been envisaged and there is an increasing interest of using L. lactis as a cell factory. (biomedcentral.com)
  • Among them, it is worth mentioning several metabolic engineering strategies to re-route L. lactis metabolism to the production of metabolites of interest for both food and pharmaceutical industries. (biomedcentral.com)
  • lactis IL1403 was studied at constant growth rate using D-stat cultivation technique. (springer.com)
  • Even S, Lindley ND, Cocaign-Bousquet M (2001) Molecular physiology of sugar catabolism in Lactococcus lactis IL1403. (springer.com)
  • The positive effects were observed from cultivation of L. lactis IL1403 with trehalose as a substrate under aerated conditions. (dtu.dk)
  • lactis strain IL1403, and confirm precise deletion of all prophages. (inrae.fr)
  • Consistent with the results from comparative genomics (see references above) this resulted in L. lactis losing or down-regulating genes which are dispensable in milk and the up-regulation of peptide transport. (wikipedia.org)
  • Dressaire C, Redon E, Milhem H, Besse P, Loubière P, Cocaign-Bousquet M (2008) Growth rate regulated genes and their wide involvement in the Lactococcus lactis stress responses. (springer.com)
  • The genes expressing these proteins were transcribed under control of the L. lactis Usp45 promoter and their products contain the Usp45 signal sequences. (frontiersin.org)
  • We established a procedure for robust estimation of the ribosomal density in hundreds of genes from Lactococcus lactis polysome size measurements. (epfl.ch)
  • The system allows efficient but controlled expression of recombinant genes in L. lactis cells, including toxic proteins, on lab and industrial scales [19,20]. (thefreelibrary.com)
  • Only three of the four type 3 CPS biosynthesis genes were found to be necessary for the abundant formation (120 mg liter −1 ) of an extracellular type 3 CPS in L. lactis , implying a role for the type 3-specific synthase in the extracellular transport of the CPS or implying the existence of an alternative export system in L. lactis . (asm.org)
  • These findings show that L. lactis is a useful host in which to study the role and function of genes involved in the production of bacterial capsules. (asm.org)
  • When we analyzed mutants with disruptions in clpB , clpC , or clpE , we found that although the genes are part of the L. lactis heat shock stimulon, the mutants responded like wild-type cells to heat and salt treatments. (pubmedcentralcanada.ca)
  • Examination of the twelve plasmids of L. lactis FM03P showed that 10 of the plasmids carry putative genes known to be important for growth and survival in the dairy environment. (biomedcentral.com)
  • Analysis of genomes of L. lactis has shown that these traits are often encoded by genes located on plasmids [ 11 ]. (biomedcentral.com)
  • Southern hybridization analysis indicated that genes homologous to ftsH of L. lactis were also present in Bacillus subtilis , and several Lactobacillus and Leuconostoc species, suggesting high conservation of ftsH in bacterial species. (microbiologyresearch.org)
  • Lactococcus lactis is a commonly used starter strain that can be converted from a vitamin B2 consumer into a vitamin B2 'factory' by over-expressing its riboflavin biosynthesis genes. (ucc.ie)
  • Heterologous gene expression of bovine plasmin in Lactococcus lactis. (nih.gov)
  • In our study, we cloned the codon-optimized gene encoding haemagglutinin of the H5N1 influenza virus and expressed it intracellularly using the nisin-controlled gene expression (NICE[R]) system in L. lactis NZ9000 strain. (thefreelibrary.com)
  • To achieve the food-grade expression of bile salt hydrolase (BSH, EC 3.5.1.24) from Lactobacillus plantarum BBE7, the nisin controlled gene expression system (NICE), food-grade selection maker and signal peptide of Lactococcus lactis were used in this study. (eurekaselect.com)
  • Many heterologous proteins have been produced in L.lactis but very few report the whole system of the gene expression machinery and its application. (alliedacademies.org)
  • Here, we review the complete gene expression system for the L.lactis right from the promoters, roll of signal peptides, site of expression and specific protein targeting system. (alliedacademies.org)
  • In recent years, scientists have developed a means of using L. lactis for nisin-controlled gene expression (NICE). (balanceone.com)
  • The Nisin controlled gene expression system (NICE ), developed at NIZO Food Research, NL, is a fully food-grade system used for controlled gene expression in Lactococcus Lactis . (bocascientific.com)
  • 2005). ' Industrial-scale production and purification of a heterologous protein in Lactococcus lactis using the nisin-controlled gene expression system NICE: the case of lysostaphin . (bocascientific.com)
  • Many different expression systems of L. lactis have been developed and used for heterologous protein expression. (wikipedia.org)
  • In this project, we investigate, by proteomics, regulation of protein synthesis and turnover in Lactococcus lactis under several different conditions. (wur.nl)
  • Oral Vaccination with the Porcine Rotavirus VP4 Outer Capsid Protein Expressed by Lactococcus lactis Induces Specific Antibody Production," Journal of Biomedicine and Biotechnology , vol. 2010, Article ID 708460, 9 pages, 2010. (hindawi.com)
  • Expression of SARS-coronavirus nucleocapsid protein in Escherichia coli and Lactococcus lactis for serodiagnosis and mucosal vaccination. (ebscohost.com)
  • Zixing Dong, Juan Zhang, Huazhong Li, Guocheng Du, Jian Chen and Byonghoon Lee, "Codon and Propeptide Optimizations to Improve the Food-grade Expression of Bile Salt Hydrolase in Lactococcus lactis ", Protein & Peptide Letters (2015) 22: 727. (eurekaselect.com)
  • This study, which used L. lactis for the production and delivery of Hsp65, has scientific relevance because it shows the efficacy of this new strategy based on therapeutic protein delivery into mammalian enterocytes. (physiciansweekly.com)
  • Lactococcus lactis, a model lactic acid bacterium that is widely used in the dairy industry, which proves beneficial due to its easy protein secretion and purification. (alliedacademies.org)
  • Lactococcus lactis , becoming the most suitable expression cell factory these days for the heterologous protein secretion. (alliedacademies.org)
  • In particular, use of an interleukin-10-secreting L. lactis to treat Crohn's disease has passed phase I clinical trials [ 13 ], indicating that L. lactis is indeed a safe mucosal vector for functional protein delivery. (biomedcentral.com)
  • A gene, encoding a protein homologous to an essential Escherichia coli protein, FtsH, was identified adjacent to the hpt gene and the trnA operon in the Gram-positive bacterium Lactococcus lactis. (microbiologyresearch.org)
  • In-frame fusions of L. lactis ftsH and phoA1 in E. coli , and immunodetection of the L. lactis FtsH protein in cell fractions using anti- E. coli FtsH serum showed that L. lactis ftsH was expressed and encodes a membrane protein. (microbiologyresearch.org)
  • In this study, the glycosylated murine Tyrosinase related protein-2 (TRP-2) with the ability to anchor onto the L. lactis cell wall was produced in suspension adapted Chinese Hamster Ovary (CHO-S) cells by expressing TRP-2 fused with cell wall anchoring LysM motif (cA) at the C-terminus. (biomedcentral.com)
  • The purified TRP-2-cA protein was shown to be N-glycosylated and successfully anchored to the L. lactis cell wall. (biomedcentral.com)
  • AggL is a new protein belonging to the collagen-binding superfamily of proteins and is sufficient for cell aggregation in lactococci. (biomedcentral.com)
  • 2015). ' Lactococcus lactis as an oral vector for cloning of heat shock protein A from Helicobacter pylori . (bocascientific.com)
  • Membrane protein expression in Lactococcus lactis. (bocascientific.com)
  • 2012). 'Heterologous protein expression by Lactococcus lactis. (bocascientific.com)
  • Studies showed that food-grade Lactococcus lactis ( L. lactis ) and NICE expression system have superior performance in exogenous protein expression. (biomedcentral.com)
  • Ekspresi dan Purifikasi Protein Inti Virus Hepatitis B (HBcAg) pada Lactococcus lactis. (ipb.ac.id)
  • lactis may be a useful tool for preventing infections by multiple bacterial species. (balanceone.com)
  • Heterologous production of bovine plasmin was studied in the industrially relevant bacterium Lactococcus lactis. (nih.gov)
  • The food-grade bacterium Lactococcus lactis has been extensively investigated during the last two decades as a delivery vector for therapeutic proteins, DNA and vaccine antigens. (ebscohost.com)
  • A gene encoding 6-phosphogluconate dehydrogenase (6-PGDH, EC 1.1.1.44) was identified from the homofermentative lactic acid bacterium Lactococcus lactis , by complementation of Escherichia coli mutants. (biochemj.org)
  • With this work, we have developed and extensively validated standard protocols for enzyme activity measurements for L. lactis. (nih.gov)
  • In this work we tested the usefulness of Lactococcus lactis , the most extensively studied lactic acid bacterium, as a delivery vector for Campylobacter antigens. (frontiersin.org)
  • Lactococcus lactis is a lactic acid bacterium which is extensively used in food fermentation processes. (biomedcentral.com)
  • Lactococcus lactis is widely used as a dairy starter and has been extensively studied. (biomedcentral.com)
  • Due to its main role in food fermentation, L. lactis has been extensively studied and has become a model lactic acid bacterium. (biomedcentral.com)
  • Lactococcus lactis is a spherical-shaped, Gram-positive bacterium extensively used for industrial production of fermented dairy products such as milk, cheese, and yogurt. (meteoricbiopharma.com)
  • In this work, we have assessed the ability of the bacteriocin Lactococcin 972 (Lcn972) gene cluster to behave as a food-grade post-segregational killing system to stabilize recombinant plasmids in L. lactis in the absence of antibiotics. (biomedcentral.com)
  • The effects were observed when cultivating plant isolates of L. lactis on xylose. (dtu.dk)
  • Based on the partial 16S rRNA sequences, the selected LAB isolates belonged to a member of Lactococcus lactis with 98% DNA similarity. (ajol.info)
  • Some of the plasmids were easily lost demonstrating that propagation outside the dairy environment should be minimised when studying dairy isolates of L. lactis . (biomedcentral.com)
  • From the four isolates that showed inhibitory activity against Lactococcus garvieae 03/8460, strain TW34 was selected because it exerted the strongest inhibition. (semanticscholar.org)
  • L. lactis strain NZ9000 ( 6 ) was cultivated semianaerobically at 30°C in M17 broth, pH 6.5, supplemented with 1.0% (wt/vol) glucose (GM17 medium) and 5 μg/ml chloramphenicol when carrying pNZopuAhis or derivatives. (pnas.org)
  • A recombinant L. lactis NZ9000-401-kiss1 successfully expressing the human kiss1 was constructed. (biomedcentral.com)
  • Soluble RANKL expression in Lactococcus lactis and investigation of its potential as an oral vaccine adjuvant. (harvard.edu)
  • In order to evaluate the impact of antigen location on vaccine prototype efficacy, a L. lactis strain producing cytoplasm-located rCjaAD was also generated. (frontiersin.org)
  • Additionally, L. lactis shows potential as a host for the safe production of capsule antigens and as a vaccine delivery vehicle for polysaccharide antigens. (asm.org)
  • The Generally Regarded as Safe (GRAS) status of the Lactococcus lactis coupled with a non-recombinant strategy of in-trans surface display, provide a safe platform for therapeutic drug and vaccine development. (biomedcentral.com)
  • Besides the production of fermented dairy products, Lactococcus lactis is now being utlitized for biotechnological applications in genetic engineering for the production of various recombinant proteins and metabolites, particularly in vaccine delivery systems. (balanceone.com)
  • Biosynthetic characterization and biochemical features of the third natural nisin variant, nisin Q, produced by Lactococcus lactis 61-14. (biomedsearch.com)
  • Characterization of the nisin gene cluster nisABTCIPR of Lactococcus lactis. (bocascientific.com)
  • Based on its history in food fermentation, L. lactis has generally recognized as safe (GRAS) status, with few case reports of it being an opportunistic pathogen. (wikipedia.org)
  • lactis 140 NWC was studied during batch fermentation in a lactose-based complex medium. (springer.com)
  • In this study, several models were developed to describe biomass, nisin, and lactic acid production as well as sucrose consumption during nisin fermentation by L. lactis in suspended-cell batch culture. (elsevier.com)
  • Lactococcus lactis is manufactured through a fermentation process and freeze-dried at GMP certified plant. (dbi360.com)
  • BACKGROUND: Lactococcus lactis is used in dairy food fermentation and for the efficient production of industrially relevant enzymes. (ru.nl)
  • Important industrial traits have been linked to plasmids in Lactococcus lactis . (biomedcentral.com)
  • Lactococcus lactis FM03P carries 12 plasmids important for its adaptation to the dairy environment. (biomedcentral.com)
  • Efficient production of secreted staphylococcal antigens in a non-lysing and proteolytically reduced Lactococcus lactis strain. (ebscohost.com)
  • Mucosally administered vaccines based on recombinant L. lactis cells producing various antigens were shown to evoke specific immune responses of humoral and cell-mediated type [7-10]. (thefreelibrary.com)
  • Furthermore, L. lactis-produced antigens are presented to the immune system in particulate form, which is postulated to evoke stronger mucosal response than soluble proteins [17, 18]. (thefreelibrary.com)
  • L. lactis has been shown to be particularly effective in delivering antigens that stimulate mucosal immunity to pathogens of the respiratory tract. (balanceone.com)
  • Until recently, members of the genus Lactococcus were considered opportunistic pathogens ( 1 , 2 ). (cdc.gov)
  • New insights into the complex regulation of the glycolytic pathway in Lactococcus lactis. (harvard.edu)
  • The overall aim is to gain knowledge on the trade-offs occurring in Lactococcus lactis under industrially relevant conditions and transitions. (wur.nl)
  • Background: Lactococcus lactis is industrially employed to manufacture various fermented dairy products. (uva.nl)
  • These results are the first evidence that there is phage-mediated direct transfer of DNA from S. thermophilus to L. lactis . (asm.org)
  • Phage adsorption was performed for 10 min at 42°C ( S. thermophilus ) or for 30 min at 30°C ( L. lactis ). (asm.org)
  • Phage adsorption was stopped on ice by addition of 1 ml of ice-cold thM17 or GM17 broth containing 20 mM ( S. thermophilus ) or 200 mM ( L. lactis ) trisodium citrate. (asm.org)
  • Kunming mice were immunized orally with these two recombinant L. lactis vaccines, and then antibodies of serum and intestinal mucosa, proliferation and cytokines of spleen lymphocytes were determined at different time points of postvaccination. (thefreelibrary.com)
  • Invasive Lactococcus lactis producing mycobacterial Hsp65 ameliorates intestinal inflammation in acute TNBS-induced colitis in mice by increasing the levels of the cytokine IL-10 and secretory IgA. (physiciansweekly.com)
  • Conclusion: We observed potential synergism between Resveratrol and Lactococcus lactis mainly in modulating the stom-ach and intestinal microbiota. (proteinpeptideletters.com)
  • Oral administration of pEGF-secreting L. lactis had beneficial effects on intestinal health and performance of early-weaned piglets. (biomedcentral.com)
  • Antimicrobial susceptibilities of Lactococcus lactis and Lactococcus garvieae and a proposed method to discriminate between them. (atcc.org)
  • lactis , L. piscium , and L. garvieae are recognized as the species with clinical significance for human and veterinary medicine ( 2 , 8 ). (cdc.gov)
  • lactis and L. garvieae are the two species more frequently found in human and animal infections ( 2 ). (cdc.gov)
  • The SCM of L. lactis exhibited antimicrobial activity against the periodontopathogens, whereas the proteinase K-treated SCM showed little antimicrobial activity. (go.jp)
  • To the best of our knowledge, this is the first report on the intracellular and extracellular expression of BSH using food-grade expression system, which would lay a solid foundation for large-scale production of BSH and other heterologous proteins in L. lactis. (eurekaselect.com)
  • Regions of Pfs 48/45 and Pfs 230 known to contain transmission blocking epitopes, 6C and C0, respectively, were produced in a Lactococcus lactis expression system and used in enzyme linked immunosorbent assays to determine the seroreactivity of 95 malaria patients living in the Central Region of Ghana. (biomedcentral.com)
  • The NICE Lactococcus lactis expression Strain NZ3900 , part of the NICE expression system, is a standard strain for food-grade selection based upon the ability to grow on lactose. (bocascientific.com)
  • Penelitian ini bertujuan untuk mengekspresikan gen penyandi HBcAg di dalam sel L. lactis NZ3900. (ipb.ac.id)
  • Genetic marking of Lactococcus lactis shows its survival in the human gastrointestinal tract. (springer.com)
  • Owing to the easy genetic accessibility of L. lactis the Ni sin C ontrolled gene E xpression (NICE ® ) system developed at NIZO has played a key role in this development. (mobitec.com)
  • Ribosomal binding sites from glycolytic promoters in L. lactis were placed in front of each gene. (dtu.dk)
  • An investigation into the control mechanisms of glycolytic flux in Lactococcus lactis. (sysmo-db.org)
  • A non-pathogenic species of LACTOCOCCUS found in DAIRY PRODUCTS and responsible for the souring of MILK and the production of LACTIC ACID. (harvard.edu)
  • Sequence comparison of the L. lactis 6-PGDH with the corresponding enzyme derived from the pathogenic protozoan Trypanosoma brucei and sheep liver revealed the substrate-binding residues to be identical in all three species, although the three coenzyme-binding pockets differed slightly. (biochemj.org)
  • Although Lactococcus species is mostly known as nonpathogenic, it should be kept in mind as a potential pathogen, especially in patients with gastrointestinal disorders. (jidc.org)
  • lactis JCM 5805 T , a synonym of ATCC 19435 T , is a type strain of this species. (asm.org)
  • The kinetics, control, and efficiency of nisin-induced expression directed by the nisA promoter region were studied in Lactococcus lactis with transcriptional and translational fusions to the gusA reporter gene. (psu.edu)
  • E. H. E. Ayad, A. Verheul, J. T. M. Wouters and G. Smit, "Antimicrobial-Producing Wild Lactococci Isolated from Artisanal and Non-Dairy Origins," International Dairy Journal, Vol. 12, No. 2-3, 2002, pp. 145-150. (scirp.org)
  • lactis BGKP1 was isolated from artisanal semi-hard homemade cheese and selected due to its strong auto-aggregation phenotype. (biomedcentral.com)
  • High level production of heterologous proteins in L.lactis has been obtained using lactococcal constitutive or inducible promoters. (alliedacademies.org)
  • This was first done in a study involving mice with colitis, where engineered secretion of interleukin-10 (IL-10) in L. lactis was used to treat inflammatory bowel disease . (balanceone.com)
  • Genetically modified Lactococcus lactis- A potential microbial therapeutic for acute colitis? (faisafrica.com)
  • In summary, the authors showed that the genetically modified Lactococcus lactis can facilitate the efficient delivery of IL-1Ra resulting in reduced inflammation and associated symptoms in a mouse model of ulcerative colitis. (faisafrica.com)
  • L. lactis is of crucial importance for manufacturing dairy products, such as buttermilk and cheeses. (wikipedia.org)
  • lactis (Lister) Schleifer et al. (atcc.org)
  • In addition, the L. lactis suspension had a neutralizing effect on the VSCs produced by periodontopathogens, and UV-killed L. lactis inhibited the production of IL-6 and TNF-α induced by the LPS. (go.jp)
  • Acid production rates during cheesemaking (with cook temperature of 38¬∞C) was more dependent on the individual strain than whether it was a lactis or cremoris strain. (usu.edu)
  • Next to the wealth of traditional food applications, L. lactis is increasingly used for modern biotechnological applications such as the production of recombinant proteins for food, feed, pharmacological and biocatalysis applications. (mobitec.com)
  • Furthermore, investigating biological activity of pEGF and exploring applications feasibility of combination effects of L. lactis and pEGF on early weaned piglets' production. (biomedcentral.com)
  • lactis is added to milk, the bacterium uses enzymes to produce energy molecules (ATP), from lactose. (wikipedia.org)
  • L. lactis has adapted to the dairy environment by the acquisition of important traits required for the growth on milk, such as lactose catabolism, proteinase activity, citrate utilisation and bacteriophage resistance. (biomedcentral.com)
  • In vitro inhibition of Citrobacter freundii, a red-leg syndrome associated pathogen in raniculture, by indigenous Lactococcus lactis CRL 1584. (semanticscholar.org)
  • Inhibition of human stomach cancer cell proliferation and resulting into cancer cell death can also be carried out by L. lactis. (meteoricbiopharma.com)
  • Progress in the development of mucosal vaccines based on Lactococcus lactis . (springer.com)
  • Compared with the wild-type L. lactis group, the sRANKL-LAB group showed significantly increased systemic and mucosal immune responses specific to M-BmpB. (sigmaaldrich.com)
  • Mutational analysis of the remaining four systems revealed that they are implicated in susceptibility to extreme pH, osmotic or oxidative conditions, or the regulation of phosphatase activity in L. lactis. (nih.gov)
  • The most cost-effective method for the preservation of L. lactis starter cultures is spray drying, but during this process cultures encounter heat and oxidative stress, typically resulting in low survival rates. (uva.nl)
  • Detailed kinetic model for glycolysis of L. lactis. (sysmo-db.org)
  • This report provides corroboration of evidence that Lactococcus lactis cremoris is a potential pathogen in immunocompetent adults. (biomedcentral.com)
  • Lactococcus Lactis Strain Plasma (Immuse) is a clinically researched strain of lactic acid bacterium. (nootropicsdepot.com)
  • This lactic acid bacterium is called Lactococcus Lactis Strain Plasma, the 'plasma' at the end of the name referring to the ability of this lactic acid bacterium's ability to activate PLASMAcytoid dendritic cells! (nootropicsdepot.com)