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Lactococcus lactisLactococcusNisinCheeseBacterial ProteinsMolecular Sequence DataBacteriophagesBacteriocinsGenes, BacterialSiphoviridaeGene Expression Regulation, BacterialLeuconostocLactobacillusBase SequenceDNA, BacterialFermentationPlasmidsAmino Acid SequenceKluyveromycesCloning, MolecularMilkOperonSequence Analysis, DNALactoseCultured Milk ProductsAcetoinConjugation, GeneticDiacetylOpen Reading FramesStreptococcus thermophilusFood MicrobiologyFood IndustryStreptococcus PhagesGenetic EngineeringCulture MediaPediococcusChromosomes, BacterialDairy ProductsPhosphofructokinasesRestriction MappingEscherichia coliSequence Homology, Amino AcidIndustrial MicrobiologyCholatesBacteriolysisAminopeptidasesLactobacillus plantarumCaseinsDNA Transposable ElementsTransformation, BacterialLactic AcidCell WallFood TechnologyStreptococcusPhosphoglucomutaseBifidobacteriumMicrococcal NucleaseL-Lactate DehydrogenaseLactobacillaceae
Lactococcus lactis: A non-pathogenic species of LACTOCOCCUS found in DAIRY PRODUCTS and responsible for the souring of MILK and the production of LACTIC ACID.Lactococcus: A genus of gram-positive, coccoid bacteria mainly isolated from milk and milk products. These bacteria are also found in plants and nonsterile frozen and dry foods. Previously thought to be a member of the genus STREPTOCOCCUS (group N), it is now recognized as a separate genus.Nisin: A 34-amino acid polypeptide antibiotic produced by Streptococcus lactis. It has been used as a food preservative in canned fruits and vegetables, and cheese.Cheese: A nutritious food consisting primarily of the curd or the semisolid substance formed when milk coagulates.Bacterial Proteins: Proteins found in any species of bacterium.Molecular Sequence Data: Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.Bacteriophages: Viruses whose hosts are bacterial cells.Bacteriocins: Substances elaborated by specific strains of bacteria that are lethal against other strains of the same or related species. They are protein or lipopolysaccharide-protein complexes used in taxonomy studies of bacteria.Genes, Bacterial: The functional hereditary units of BACTERIA.Siphoviridae: A family of BACTERIOPHAGES and ARCHAEAL VIRUSES which are characterized by long, non-contractile tails.Gene Expression Regulation, Bacterial: Any of the processes by which cytoplasmic or intercellular factors influence the differential control of gene action in bacteria.Leuconostoc: A genus of gram-positive, facultatively anaerobic bacteria whose growth is dependent on the presence of a fermentable carbohydrate. It is nonpathogenic to plants and animals, including humans.Lactobacillus: A genus of gram-positive, microaerophilic, rod-shaped bacteria occurring widely in nature. Its species are also part of the many normal flora of the mouth, intestinal tract, and vagina of many mammals, including humans. Pathogenicity from this genus is rare.Base Sequence: The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.DNA, Bacterial: Deoxyribonucleic acid that makes up the genetic material of bacteria.Fermentation: Anaerobic degradation of GLUCOSE or other organic nutrients to gain energy in the form of ATP. End products vary depending on organisms, substrates, and enzymatic pathways. Common fermentation products include ETHANOL and LACTIC ACID.Plasmids: Extrachromosomal, usually CIRCULAR DNA molecules that are self-replicating and transferable from one organism to another. They are found in a variety of bacterial, archaeal, fungal, algal, and plant species. They are used in GENETIC ENGINEERING as CLONING VECTORS.Amino Acid Sequence: The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.Kluyveromyces: An ascomycetous yeast of the fungal family Saccharomycetaceae, order SACCHAROMYCETALES.Cloning, Molecular: The insertion of recombinant DNA molecules from prokaryotic and/or eukaryotic sources into a replicating vehicle, such as a plasmid or virus vector, and the introduction of the resultant hybrid molecules into recipient cells without altering the viability of those cells.Milk: The white liquid secreted by the mammary glands. It contains proteins, sugar, lipids, vitamins, and minerals.Operon: In bacteria, a group of metabolically related genes, with a common promoter, whose transcription into a single polycistronic MESSENGER RNA is under the control of an OPERATOR REGION.Sequence Analysis, DNA: A multistage process that includes cloning, physical mapping, subcloning, determination of the DNA SEQUENCE, and information analysis.Lactose: A disaccharide of GLUCOSE and GALACTOSE in human and cow milk. It is used in pharmacy for tablets, in medicine as a nutrient, and in industry.Cultured Milk Products: Milk modified with controlled FERMENTATION. This should not be confused with KAFFIR LIME or with KAFFIR CORN.Acetoin: A product of fermentation. It is a component of the butanediol cycle in microorganisms. In mammals it is oxidized to carbon dioxide.Conjugation, Genetic: A parasexual process in BACTERIA; ALGAE; FUNGI; and ciliate EUKARYOTA for achieving exchange of chromosome material during fusion of two cells. In bacteria, this is a uni-directional transfer of genetic material; in protozoa it is a bi-directional exchange. In algae and fungi, it is a form of sexual reproduction, with the union of male and female gametes.Diacetyl: Carrier of aroma of butter, vinegar, coffee, and other foods.Open Reading Frames: A sequence of successive nucleotide triplets that are read as CODONS specifying AMINO ACIDS and begin with an INITIATOR CODON and end with a stop codon (CODON, TERMINATOR).Streptococcus thermophilus: A species of thermophilic, gram-positive bacteria found in MILK and milk products.Food Microbiology: The presence of bacteria, viruses, and fungi in food and food products. This term is not restricted to pathogenic organisms: the presence of various non-pathogenic bacteria and fungi in cheeses and wines, for example, is included in this concept.Food Industry: The industry concerned with processing, preparing, preserving, distributing, and serving of foods and beverages.Streptococcus Phages: Viruses whose host is Streptococcus.Genetic Engineering: Directed modification of the gene complement of a living organism by such techniques as altering the DNA, substituting genetic material by means of a virus, transplanting whole nuclei, transplanting cell hybrids, etc.Culture Media: Any liquid or solid preparation made specifically for the growth, storage, or transport of microorganisms or other types of cells. The variety of media that exist allow for the culturing of specific microorganisms and cell types, such as differential media, selective media, test media, and defined media. Solid media consist of liquid media that have been solidified with an agent such as AGAR or GELATIN.Pediococcus: A genus of gram-positive, facultatively anaerobic bacteria whose growth is dependent on the presence of a fermentable carbohydrate. No endospores are produced. Its organisms are found in fermenting plant products and are nonpathogenic to plants and animals, including humans.Chromosomes, Bacterial: Structures within the nucleus of bacterial cells consisting of or containing DNA, which carry genetic information essential to the cell.Dairy Products: Raw and processed or manufactured milk and milk-derived products. These are usually from cows (bovine) but are also from goats, sheep, reindeer, and water buffalo.Phosphofructokinases: Allosteric enzymes that regulate glycolysis and gluconeogenesis. These enzymes catalyze phosphorylation of fructose-6-phosphate to either fructose-1,6-bisphosphate (PHOSPHOFRUCTOKINASE-1 reaction), or to fructose-2,6-bisphosphate (PHOSPHOFRUCTOKINASE-2 reaction).Restriction Mapping: Use of restriction endonucleases to analyze and generate a physical map of genomes, genes, or other segments of DNA.Escherichia coli: A species of gram-negative, facultatively anaerobic, rod-shaped bacteria (GRAM-NEGATIVE FACULTATIVELY ANAEROBIC RODS) commonly found in the lower part of the intestine of warm-blooded animals. It is usually nonpathogenic, but some strains are known to produce DIARRHEA and pyogenic infections. Pathogenic strains (virotypes) are classified by their specific pathogenic mechanisms such as toxins (ENTEROTOXIGENIC ESCHERICHIA COLI), etc.Sequence Homology, Amino Acid: The degree of similarity between sequences of amino acids. This information is useful for the analyzing genetic relatedness of proteins and species.Industrial Microbiology: The study, utilization, and manipulation of those microorganisms capable of economically producing desirable substances or changes in substances, and the control of undesirable microorganisms.Cholates: Salts and esters of CHOLIC ACID.Bacteriolysis: Rupture of bacterial cells due to mechanical force, chemical action, or the lytic growth of BACTERIOPHAGES.Aminopeptidases: A subclass of EXOPEPTIDASES that act on the free N terminus end of a polypeptide liberating a single amino acid residue. EC 3.4.11.Lactobacillus plantarum: A species of rod-shaped, LACTIC ACID bacteria used in PROBIOTICS and SILAGE production.Caseins: A mixture of related phosphoproteins occurring in milk and cheese. The group is characterized as one of the most nutritive milk proteins, containing all of the common amino acids and rich in the essential ones.DNA Transposable Elements: Discrete segments of DNA which can excise and reintegrate to another site in the genome. Most are inactive, i.e., have not been found to exist outside the integrated state. DNA transposable elements include bacterial IS (insertion sequence) elements, Tn elements, the maize controlling elements Ac and Ds, Drosophila P, gypsy, and pogo elements, the human Tigger elements and the Tc and mariner elements which are found throughout the animal kingdom.Transformation, Bacterial: The heritable modification of the properties of a competent bacterium by naked DNA from another source. The uptake of naked DNA is a naturally occuring phenomenon in some bacteria. It is often used as a GENE TRANSFER TECHNIQUE.Lactic Acid: A normal intermediate in the fermentation (oxidation, metabolism) of sugar. The concentrated form is used internally to prevent gastrointestinal fermentation. (From Stedman, 26th ed)Cell Wall: The outermost layer of a cell in most PLANTS; BACTERIA; FUNGI; and ALGAE. The cell wall is usually a rigid structure that lies external to the CELL MEMBRANE, and provides a protective barrier against physical or chemical agents.Food Technology: The application of knowledge to the food industry.Streptococcus: A genus of gram-positive, coccoid bacteria whose organisms occur in pairs or chains. No endospores are produced. Many species exist as commensals or parasites on man or animals with some being highly pathogenic. A few species are saprophytes and occur in the natural environment.Phosphoglucomutase: An enzyme that catalyzes the conversion of alpha D-glucose 1-phosphate to alpha D-glucose 6-phosphate. EC 5.4.2.2.Bifidobacterium: A rod-shaped, gram-positive, non-acid-fast, non-spore-forming, non-motile bacterium that is a genus of the family Bifidobacteriaceae, order Bifidobacteriales, class ACTINOBACTERIA. It inhabits the intestines and feces of humans as well as the human vagina.Micrococcal Nuclease: An enzyme that catalyzes the endonucleolytic cleavage to 3'-phosphomononucleotide and 3'-phospholigonucleotide end-products. It can cause hydrolysis of double- or single-stranded DNA or RNA. (From Enzyme Nomenclature, 1992) EC 3.1.31.1.L-Lactate Dehydrogenase: A tetrameric enzyme that, along with the coenzyme NAD+, catalyzes the interconversion of LACTATE and PYRUVATE. In vertebrates, genes for three different subunits (LDH-A, LDH-B and LDH-C) exist.Lactobacillaceae: A family of gram-positive bacteria found regularly in the mouth and intestinal tract of man and other animals, in food and dairy products, and in fermenting vegetable juices. A few species are highly pathogenic.

Novel characteristic for distinguishing Lactococcus lactis subsp. lactis from subsp. cremoris. (1/1459)

Lactococcus lactis strains were examined for their ability to produce gamma-aminobutyric acid (GABA). Results showed that strains of L. lactis subsp. lactis were able to produce this acid, whereas L. lactis subsp. cremoris were not. GABA production thus represents another effective characteristic for distinguishing L. lactis subsp. lactis from L. lactis subsp. cremoris.  (+info)

Mechanism of citrate metabolism in Lactococcus lactis: resistance against lactate toxicity at low pH. (2/1459)

Measurement of the flux through the citrate fermentation pathway in resting cells of Lactococcus lactis CRL264 grown in a pH-controlled fermentor at different pH values showed that the pathway was constitutively expressed, but its activity was significantly enhanced at low pH. The flux through the citrate-degrading pathway correlated with the magnitude of the membrane potential and pH gradient that were generated when citrate was added to the cells. The citrate degradation rate and proton motive force were significantly higher when glucose was metabolized at the same time, a phenomenon that could be mimicked by the addition of lactate, the end product of glucose metabolism. The results clearly demonstrate that citrate metabolism in L. lactis is a secondary proton motive force-generating pathway. Although the proton motive force generated by citrate in cells grown at low pH was of the same magnitude as that generated by glucose fermentation, citrate metabolism did not affect the growth rate of L. lactis in rich media. However, inhibition of growth by lactate was relieved when citrate also was present in the growth medium. Citrate did not relieve the inhibition by other weak acids, suggesting a specific role of the citrate transporter CitP in the relief of inhibition. The mechanism of citrate metabolism presented here provides an explanation for the resistance to lactate toxicity. It is suggested that the citrate metabolic pathway is induced under the acidic conditions of the late exponential growth phase to make the cells (more) resistant to the inhibitory effects of the fermentation product, lactate, that accumulates under these conditions.  (+info)

A general method for selection of alpha-acetolactate decarboxylase-deficient Lactococcus lactis mutants to improve diacetyl formation. (3/1459)

The enzyme acetolactate decarboxylase (Ald) plays a key role in the regulation of the alpha-acetolactate pool in both pyruvate catabolism and the biosynthesis of the branched-chain amino acids, isoleucine, leucine, and valine (ILV). This dual role of Ald, due to allosteric activation by leucine, was used as a strategy for the isolation of Ald-deficient mutants of Lactococcus lactis subsp. lactis biovar diacetylactis. Such mutants can be selected as leucine-resistant mutants in ILV- or IV-prototrophic strains. Most dairy lactococcus strains are auxotrophic for the three amino acids. Therefore, the plasmid pMC004 containing the ilv genes (encoding the enzymes involved in the biosynthesis of IV) of L. lactis NCDO2118 was constructed. Introduction of pMC004 into ILV-auxotrophic dairy strains resulted in an isoleucine-prototrophic phenotype. By plating the strains on a chemically defined medium supplemented with leucine but not valine and isoleucine, spontaneous leucine-resistant mutants were obtained. These mutants were screened by Western blotting with Ald-specific antibodies for the presence of Ald. Selected mutants lacking Ald were subsequently cured of pMC004. Except for a defect in the expression of Ald, the resulting strain, MC010, was identical to the wild-type strain, as shown by Southern blotting and DNA fingerprinting. The mutation resulting in the lack of Ald in MC010 occurred spontaneously, and the strain does not contain foreign DNA; thus, it can be regarded as food grade. Nevertheless, its application in dairy products depends on the regulation of genetically modified organisms. These results establish a strategy to select spontaneous Ald-deficient mutants from transformable L. lactis strains.  (+info)

Characterization of the divergent sacBK and sacAR operons, involved in sucrose utilization by Lactococcus lactis. (4/1459)

The divergently transcribed sacBK and sacAR operons, which are involved in the utilization of sucrose by Lactococcus lactis NZ9800, were examined by transcriptional and gene inactivation studies. Northern analyses of RNA isolated from cells grown at the expense of different carbon sources revealed three sucrose-inducible transcripts: one of 3.2 kb containing sacB and sacK, a second of 3.4 kb containing sacA and sacR, and a third of 1.8 kb containing only sacR. The inactivation of the sacR gene by replacement recombination resulted in the constitutive transcription of the sacBK and sacAR operons in the presence of different carbon sources, indicating that SacR acts as a repressor of transcription.  (+info)

Membrane topology of the lactococcal bacteriocin ATP-binding cassette transporter protein LcnC. Involvement of LcnC in lactococcin a maturation. (5/1459)

Many non-lantibiotic bacteriocins of lactic acid bacteria are produced as precursors with N-terminal leader peptides different from those present in preproteins exported by the general sec-dependent (type II) secretion pathway. These bacteriocins utilize a dedicated (type I) secretion system for externalization. The secretion apparatus for the lactococcins A, B, and M/N (LcnA, B, and M/N) from Lactococcus lactis is composed of the two membrane proteins LcnC and LcnD. LcnC belongs to the ATP-binding cassette transporters, whereas LcnD is a protein with similarities to other accessory proteins of type I secretion systems. This paper shows that the N-terminal part of LcnC is involved in the processing of the precursor of LcnA. By making translational fusions of LcnC to the reporter proteins beta-galactosidase (LacZ) and alkaline phosphatase (PhoA*), it was shown that both the N- and C-terminal parts of LcnC are located in the cytoplasm. As the N terminus of LcnC is required for LcnA maturation and is localized in the cytoplasm, we conclude that the processing of the bacteriocin LcnA to its mature form takes place at the cytosolic side of the cytoplasmic membrane.  (+info)

Hydrolysis of alphas1- and beta-casein-derived peptides with a broad specificity aminopeptidase and proline specific aminopeptidases from Lactococcus lactis subsp. cremoris AM2. (6/1459)

Aminopeptidase hydrolysis of alpha(s)1 - and beta-casein-derived synthetic peptides containing non-consecutive and consecutive proline residues was characterised. Aminopeptidase P (Pep P) (EC 3.4.11.9) or post-proline dipeptidyl aminopeptidase (PPDA) (EC 3.4.14.5) along with lysine-paranitroanilide hydrolase (KpNA-H) (EC 3.4.11.1) activities are required in the degradation of peptides containing non-consecutive proline residues. However, both Pep P and PPDA along with KpNA-H are required for hydrolysis of peptides containing consecutive proline residues. The results demonstrate the mechanism by which combinations of purified general and proline specific aminopeptidases from Lactococcus lactis subsp. cremoris AM2 hydrolyse peptides containing proline residues.  (+info)

Regulation of expression of the Lactococcus lactis histidine operon. (7/1459)

In Lactococcus lactis, the his operon contains all the genes necessary for histidine biosynthesis. It is transcribed from a unique promoter, localized 300 bp upstream of the first gene. The region corresponding to the untranslated 5' end of the transcript, named the his leader region, displays the typical features of the T box transcriptional attenuation mechanism which is involved in the regulation of many amino acid biosynthetic operons and tRNA synthetase genes in gram-positive bacteria. Here we describe the regulation of transcription of the his operon by the level of histidine in the growth medium. In the absence of histidine, two transcripts are present. One covers the entire operon, while the other stops at a terminator situated about 250 bp downstream of the transcription start point. DNA sequences implicated in regulation of the his operon were identified by transcriptional fusion with luciferase genes and site-directed mutagenesis. In addition to the previously defined sequences necessary for effective T-box-mediated regulation, new essential regions were identified. Eighteen percent of the positions of the his leader region were found to differ in seven distantly related strains of L. lactis. Analysis of the variable positions supports the folding model of the central part of the his leader region. Lastly, in addition to the T-box-mediated regulation, the operon is regulated at the level of initiation of transcription, which is repressed in the presence of histidine. An operator site, necessary for full repression, overlaps the terminator involved in the T box attenuation mechanism. The functionality of the operator is altered on plasmids with low and high copy numbers, suggesting that supercoiling may play a role in the expression of the his operon. The extents of regulation at the levels of initiation and attenuation of transcription are 6- to 8-fold and 14-fold, respectively. Together, the two levels of control allow a 120-fold range of regulation of the L. lactis operon by histidine.  (+info)

Disruption and analysis of the clpB, clpC, and clpE genes in Lactococcus lactis: ClpE, a new Clp family in gram-positive bacteria. (8/1459)

In the genome of the gram-positive bacterium Lactococcus lactis MG1363, we have identified three genes (clpC, clpE, and clpB) which encode Clp proteins containing two conserved ATP binding domains. The proteins encoded by two of the genes belong to the previously described ClpB and ClpC families. The clpE gene, however, encodes a member of a new Clp protein family that is characterized by a short N-terminal domain including a putative zinc binding domain (-CX2CX22CX2C-). Expression of the 83-kDa ClpE protein as well as of the two proteins encoded by clpB was strongly induced by heat shock and, while clpC mRNA synthesis was moderately induced by heat, we were unable to identify the ClpC protein. When we analyzed mutants with disruptions in clpB, clpC, or clpE, we found that although the genes are part of the L. lactis heat shock stimulon, the mutants responded like wild-type cells to heat and salt treatments. However, when exposed to puromycin, a tRNA analogue that results in the synthesis of truncated, randomly folded proteins, clpE mutant cells formed smaller colonies than wild-type cells and clpB and clpC mutant cells. Thus, our data suggest that ClpE, along with ClpP, which recently was shown to participate in the degradation of randomly folded proteins in L. lactis, could be necessary for degrading proteins generated by certain types of stress.  (+info)

Modeling of nisin production by Lactococcus lactis</em>...Modeling of nisin production by Lactococcus lactis</em>...
TY - CONF. T1 - Modeling of nisin production by Lactococcus lactis. AU - Pongtharangkul, Thunyarat. AU - Demirci, Ali. AU - Puri, Virendra. PY - 2007/11/7. Y1 - 2007/11/7. N2 - Nisin, the only FDA approved bacteriocin, is widely used as a natural food preservative against Gram-positive bacteria. It is produced by lactic acid bacteria Lactococcus lactis subsp. lactis during fermentation. Mathematical models of biomass and nisin production during fermentation not only provide information about the kinetic-metabolic nature of nisin, but also facilitate the control and optimization of nisin production. In this study, several models were developed to describe biomass, nisin, and lactic acid production as well as sucrose consumption during nisin fermentation by L. lactis in suspended-cell batch culture. All models were designed so that they contained biologically relevant parameters. The re-modified logistic and Gompertz models proposed in this study adequately described the growth and the validation ...
Isolation and properties of Lactococcus lactis subsp. lactis biovar diacetylactis CNRZ 483 mutants producing diacetyl and...Isolation and properties of Lactococcus lactis subsp. lactis biovar diacetylactis CNRZ 483 mutants producing diacetyl and...
Following treatment with the mutagen N-methyl-N-nitro-N-nitrosoguanidine, three mutants of Lactococcus lactis subsp. lactis biovar diacetylactis CNRZ 483 that produced diacetyl and acetoin from glucose were isolated. The lactate dehydrogenase activity of these mutants was strongly attenuated, and the mutants produced less lactate than the parental strain. The kinetic properties of lactate dehydrogenase of strain CNRZ 483 and the mutants revealed differences in the affinity of the enzyme for pyruvate, NADH, and fructose-1,6-diphosphate. When cultured aerobically, strain CNRZ 483 transformed 2.3% of glucose to acetoin and produced no diacetyl or 2,3-butanediol. Under the same conditions, mutants 483L1, 483L2, and 483L3 transformed 42.0, 78.9, and 75.8%, respectively, of glucose to C4 compounds (diacetyl, acetoin, and 2,3-butanediol). Anaerobically, strain CNRZ 483 produced no C4 compounds, while mutants 483L1, 483L2, and 483L3 transformed 2.0, 37.0, and 25.8% of glucose to acetoin and 2,3-butanediol. In
Draft Genome Sequences of Three Amino Acid-Secreting Lactococcus lactis Strains - Research database - University of GroningenDraft Genome Sequences of Three Amino Acid-Secreting Lactococcus lactis Strains - Research database - University of Groningen
TY - JOUR. T1 - Draft Genome Sequences of Three Amino Acid-Secreting Lactococcus lactis Strains. AU - Hernandez-Valdes, Jhonatan A.. AU - de Jong, Anne. AU - Kok, Jan. AU - Kuipers, Oscar P.. N1 - Copyright © 2020 Hernandez-Valdes et al.. PY - 2020/4/16. Y1 - 2020/4/16. N2 - Three Lactococcus lactis strains with the ability to secrete various amino acids (leucine, isoleucine, methionine, valine, glutamic acid, and histidine) were sequenced in order to identify the mechanisms involved in the secretion. Amino acids contribute to flavor formation; therefore, bacterial strains with this ability are relevant for the food industry.. AB - Three Lactococcus lactis strains with the ability to secrete various amino acids (leucine, isoleucine, methionine, valine, glutamic acid, and histidine) were sequenced in order to identify the mechanisms involved in the secretion. Amino acids contribute to flavor formation; therefore, bacterial strains with this ability are relevant for the food industry.. KW - ...
6-Phosphogluconate dehydrogenase from Lactococcus lactis: a role for arginine residues in binding substrate and coenzyme |...6-Phosphogluconate dehydrogenase from Lactococcus lactis: a role for arginine residues in binding substrate and coenzyme |...
A gene encoding 6-phosphogluconate dehydrogenase (6-PGDH, EC 1.1.1.44) was identified from the homofermentative lactic acid bacterium Lactococcus lactis, by complementation of Escherichia coli mutants. The cloned gene was then expressed to high levels in E. coli and the protein purified for kinetic analysis. The enzyme had a Km for 6-phosphogluconate of 15.4±1.4 µM and for NADP of 1.9±0.2 µM at pH 7.5. Sequence comparison of the L. lactis 6-PGDH with the corresponding enzyme derived from the pathogenic protozoan Trypanosoma brucei and sheep liver revealed the substrate-binding residues to be identical in all three species, although the three coenzyme-binding pockets differed slightly. A totally conserved arginine residue (Arg-447), believed to bind the 6-phosphate of substrate, was mutated to lysine, aspartate, alanine or tryptophan. In each case enzyme activity was lost, confirming an essential role for this residue on activity. A second arginine (Arg-34), believed to be critical in binding ...
Two different pathways for D-xylose metabolism and the effect of xylose concentration on the yield coefficient of L-lactate in...Two different pathways for D-xylose metabolism and the effect of xylose concentration on the yield coefficient of L-lactate in...
TY - JOUR. T1 - Two different pathways for D-xylose metabolism and the effect of xylose concentration on the yield coefficient of L-lactate in mixed-acid fermentation by the lactic acid bacterium Lactococcus lactis IO-1. AU - Tanaka, K.. AU - Komiyama, A.. AU - Sonomoto, K.. AU - Ishizaki, A.. AU - Hall, S.. AU - Stanbury, P.. PY - 2002/10. Y1 - 2002/10. N2 - In lactic acid bacteria, pentoses are metabolized via the phosphoketolase pathway, which catalyzes the cleavage of D-xylulose-5-phosphate to equimolar amounts of glyceraldehyde 3-phosphate and acetylphosphate. Hence the yield coefficient of lactate from pentose does not exceed 1.0 mol/mol, while that of Lactococcus lactis IO-1(JCM7638) at high D-xylose concentrations often exceeds the theoretical value. This suggests that, in addition to the phosphoketolase pathway, L. lactis IO-1 may possess another metabolic pathway that produces only lactic acid from xylose. In the present study, the metabolism of xylose in L. lactis IO-1 was deduced ...
Cloning and expression of a novel lactococcal aggregation factor from Lactococcus lactis subsp. lactis BGKP1 | BMC Microbiology...Cloning and expression of a novel lactococcal aggregation factor from Lactococcus lactis subsp. lactis BGKP1 | BMC Microbiology...
The aggregation phenomenon in lactococci has been studied for more than thirty years. It was found to be directly associated with a sex factor and lactose plasmid co-integration event [1] or duplication of the cell wall spanning (CWS) domain of PrtP proteinase [2]. Lactose plasmid conjugation in Lactococcus lactis 712 and in the related strains C2 and ML3, frequently involves plasmid co-integration with a sex factor. Moreover, this phenomenon is often associated with a cell aggregation phenotype and high frequency transfer ability [3-5]. The lactococcal sex factor exists integrated in the chromosome [6], although it can be excised as a closed circular form and lost from the cell [1]. Deletion and over-expression experiments confirmed that CluA is the only sex factor component responsible for aggregation in L. lactis. This 136 kDa surface-bound protein, encoded by the chromosomally located sex factor of Lactococcus lactis subsp. cremoris MG1363, is associated with cell aggregation linked to ...
Recombinant porcine epidermal growth factor-secreting Lactococcus lactis promotes the growth performance of early-weaned...Recombinant porcine epidermal growth factor-secreting Lactococcus lactis promotes the growth performance of early-weaned...
Epidermal growth factor (EGF) is an important growth factor in regulation of cell proliferation, differentiation, survival and apoptosis. Studies showed that food-grade Lactococcus lactis (L. lactis) and NICE expression system have superior performance in exogenous protein expression. This study aimed to construct and express porcine EGF (pEGF), and use L. lactis as vehicle for producing and delivering pEGF. Furthermore, investigating biological activity of pEGF and exploring applications feasibility of combination effects of L. lactis and pEGF on early weaned piglets production. A recombinant Lactococcus lactis which produced and secreted pEGF at 1000 ng/ml in culture supernatant was generated. Secreted pEGF was a fully biologically active protein, as demonstrated by its capacity to stimulate L929 mouse fibroblast cell line proliferation in vitro. For in vivo study, forty piglets were randomly allocated to control, antibiotic control, empty vector-expressing L. lactis (LL-EV) and pEGF-secreting L.
Immunopathological evaluation of recombinant mycobacterial antigen Hsp65 expressed in Lactococcus lactis as a novel vaccine...Immunopathological evaluation of recombinant mycobacterial antigen Hsp65 expressed in Lactococcus lactis as a novel vaccine...
Bovine tuberculosis (TBB) is a zoonotic disease distributed worldwide and is of great importance for public health and the livestock industry. Several experimental vaccines against this disease have been evaluated in recent years, yielding varying results. An example is the Bacillus Calmette-Guérin (BCG) vaccine, which has been used extensively in humans and tested in cattle showing mixed results related to protection (0-80%) against Mycobacterium bovis. In this study, we used the food-grade bacterium Lactococcus lactis as an expression system for production of mycobacterial protein Hsp65. For this purpose, the construction of a replicable plasmid in strain NZ9000 L. lactis (pVElepr) was conducted, which expressed the Mycobacterium leprae Hsp65 antigen, and was recognized by traded anti-Hsp65 antibodies. The strain NZ9000-pVElepr was applied to calves that were negative to tuberculin test and the immune response was monitored. The results showed that immune response was not significantly increased in
UniProt/SWISS-PROT: KITH LACLAUniProt/SWISS-PROT: KITH LACLA
ID KITH_LACLA Reviewed; 189 AA. AC Q9CHX5; DT 31-OCT-2003, integrated into UniProtKB/Swiss-Prot. DT 01-JUN-2001, sequence version 1. DT 07-JUN-2017, entry version 82. DE RecName: Full=Thymidine kinase {ECO:0000255,HAMAP-Rule:MF_00124}; DE EC=2.7.1.21 {ECO:0000255,HAMAP-Rule:MF_00124}; GN Name=tdk {ECO:0000255,HAMAP-Rule:MF_00124}; OrderedLocusNames=LL0593; GN ORFNames=L184871; OS Lactococcus lactis subsp. lactis (strain IL1403) (Streptococcus OS lactis). OC Bacteria; Firmicutes; Bacilli; Lactobacillales; Streptococcaceae; OC Lactococcus. OX NCBI_TaxID=272623; RN [1] RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=IL1403; RX PubMed=11337471; DOI=10.1101/gr.GR-1697R; RA Bolotin A., Wincker P., Mauger S., Jaillon O., Malarme K., RA Weissenbach J., Ehrlich S.D., Sorokin A.; RT "The complete genome sequence of the lactic acid bacterium Lactococcus RT lactis ssp. lactis IL1403."; RL Genome Res. 11:731-753(2001). CC -!- CATALYTIC ACTIVITY: ATP + thymidine = ADP + thymidine 5- CC phosphate. ...
The Transcriptional and Gene Regulatory Network of Lactococcus lactis MG1363 during Growth in Milk - Danish National Research...The Transcriptional and Gene Regulatory Network of Lactococcus lactis MG1363 during Growth in Milk - Danish National Research...
In the present study we examine the changes in the expression of genes of Lactococcus lactis subspecies cremoris MG1363 during growth in milk. To reveal which specific classes of genes (pathways, operons, regulons, COGs) are important, we performed a transcriptome time series experiment. Global analysis of gene expression over time showed that L. lactis adapted quickly to the environmental changes. Using upstream sequences of genes with correlated gene expression profiles, we uncovered a substantial number of putative DNA binding motifs that may be relevant for L. lactis fermentative growth in milk. All available novel and literature-derived data were integrated into network reconstruction building blocks, which were used to reconstruct and visualize the L. lactis gene regulatory network. This network enables easy mining in the chrono-transcriptomics data. A freely available website at http://milkts.molgenrug.nl gives full access to all transcriptome data, to the reconstructed network and to the ...
Further elucidation of galactose utilization in Lactococcus lactis MG1363Further elucidation of galactose utilization in Lactococcus lactis MG1363
Since the 1970s, galactose metabolism in Lactococcus lactis has been in debate. Different studies led to diverse outcomes making it difficult to conclude whether galactose uptake was PEP- or ATP-dependent and decide what the exact connection was between galactose and lactose uptake and metabolism. It was shown that some Lactococcus strains possess two galactose-specific systems - a permease and a PTS, even if they lack the lactose utilization plasmid, proving that a lactose-independent PTSGal exists. However, the PTSGal transporter was never identified. Here, with the help of transcriptome analyses and genetic knock-out mutants, we reveal the identities of two low-affinity galactose PTSs. A novel plant-niche-related PTS component Llmg_0963 forming a hybrid transporter Llmg_0963PtcBA and a glucose/mannose-specific PTS are shown to be involved in galactose transport in L. lactis MG1363 ...
Genotype-phenotype matching analysis of 38 Lactococcus lactis strains using random forest methodsGenotype-phenotype matching analysis of 38 Lactococcus lactis strains using random forest methods
BACKGROUND: Lactococcus lactis is used in dairy food fermentation and for the efficient production of industrially relevant enzymes. The genome content and different phenotypes have been determined for multiple L. lactis strains in order to understand intra-species genotype and phenotype diversity and annotate gene functions. In this study, we identified relations between gene presence and a collection of 207 phenotypes across 38 L. lactis strains of dairy and plant origin. Gene occurrence and phenotype data were used in an iterative gene selection procedure, based on the Random Forest algorithm, to identify genotype-phenotype relations. RESULTS: A total of 1388 gene-phenotype relations were found, of which some confirmed known gene-phenotype relations, such as the importance of arabinose utilization genes only for strains of plant origin. We also identified a gene cluster related to growth on melibiose, a plant disaccharide; this cluster is present only in melibiose-positive strains and can be ...
The First Clinical Use of a Recombinant Lactococcus lactis Expressing Human Papillomavirus Type 16 E7 Oncogene Oral Vaccine: A...The First Clinical Use of a Recombinant Lactococcus lactis Expressing Human Papillomavirus Type 16 E7 Oncogene Oral Vaccine: A...
Figure 2. HPV-16 E7-specific serum IgG antibody (1, top) and E7-specific vaginal IgA antibody (2, bottom) levels by placebo groups [cohorts 1 (A, left); 1 × 109 CFU/mL dose, cohorts 2 (B, center); 5 × 109 CFU/mL dose, and cohorts 3 (C, right); 1 × 1010 CFU/mL dose] and vaccine group [cohorts 4 (A, left); 1 × 109 CFU/mL dose, cohorts 5 (B, center); 5 × 109 CFU/mL dose, and cohorts 6 (C, right); 1 × 1010 CFU/mL dose] measured by ELISA at a serum dilution of 1:100 using goat anti-human IgG H&L (HRP) antibody and at a vaginal fluid dilution of 1:10 using goat anti-human IgA alpha chain (HRP) antibody, respectively. Results are expressed with 95% CIs for the following time points: prevaccination (day 0), days 30, 60, 90, and 240. The absorbance of each well was measured at 450 nm. Bars represent the mean ± standard error value of each group. Statistically significant differences are denoted by an asterisk between T60 and T30, T90, and T240 of vaccine groups (*, P ≤ 0.0001; **, P ≤ 0.001; ...
Mucosal Immunization with Recombinant Lactococcus lactis | Springer for Research & DevelopmentMucosal Immunization with Recombinant Lactococcus lactis | Springer for Research & Development
The United States National Institutes of Health has recently pointed out that despite many years of intensive vaccine research and the development of sophisticated effective vaccines, thousands of...
Genomic features of Lactococcus lactis IO-1, a lactic acid bacterium that utilizes xylose and produces high levels of L-lactic...Genomic features of Lactococcus lactis IO-1, a lactic acid bacterium that utilizes xylose and produces high levels of L-lactic...
TY - JOUR. T1 - Genomic features of Lactococcus lactis IO-1, a lactic acid bacterium that utilizes xylose and produces high levels of L-lactic acid. AU - Shimizu-Kadota, Mariko. AU - Kato, Hiroaki. AU - Shiwa, Yuh. AU - Oshima, Kenshiro. AU - Machii, Miki. AU - Araya-Kojima, Tomoko. AU - Zendo, Takeshi. AU - Hattori, Masahira. AU - Sonomoto, Kenji. AU - Yoshikawa, Hirofumi. PY - 2013. Y1 - 2013. N2 - Lactococcus lactis IO-1 (JCM7638) produces L-lactic acid predominantly when grown at high xylose concentrations, and its utilization is highly desired in the green plastics industry. Therefore it is worthwhile studying its genomic traits. In this study, we focused on (i) genes of possible horizontal transfer derivation (prophages, the nisin-sucrose transposon, and several restrictionmodification systems), and (ii) genes for the synthetic pathways of amino acids and vitamins in the IO-1 genome. In view of the results of this analysis, we consider their meanings in strain IO-1.. AB - Lactococcus ...
Lactococcus lactis - WikipediaLactococcus lactis - Wikipedia
Lactococcus lactis is a Gram-positive bacterium used extensively in the production of buttermilk and cheese, but has also become famous as the first genetically modified organism to be used alive for the treatment of human disease. L. lactis cells are cocci that group in pairs and short chains, and, depending on growth conditions, appear ovoid with a typical length of 0.5 - 1.5 µm. L. lactis does not produce spores (nonsporulating) and are not motile (nonmotile). They have a homofermentative metabolism, meaning they produce lactic acid from sugars. Theyve aso been reported to produce exclusive L-(+)-lactic acid. However, reported D-(−)-lactic acid can be produced when cultured at low pH. The capability to produce lactic acid is one of the reasons why L. lactis is one of the most important microorganisms in the dairy industry. Based on its history in food fermentation, L. lactis has generally recognized as safe (GRAS) status with few case reports of being an opportunistic pathogen. L. lactis ...
Production, secretion and purification of a correctly folded staphylococcal antigen in Lactococcus lactis | Microbial Cell...Production, secretion and purification of a correctly folded staphylococcal antigen in Lactococcus lactis | Microbial Cell...
Lactococcus lactis, a Gram-positive lactic acid bacterium and a classical starter for the manufacture of cheeses, can be used as a cell factory to produce proteins of interest [1-6]. As a long known innocuous, Generally Recognised As Safe ("GRAS") food-grade species [7], L. lactis is an interesting host to produce proteins of medical interest [2, 4, 5]. Compared to Escherichia coli, the advantage of L. lactis is that it does not produce endotoxin (lipopolysaccharide) [2, 4, 5] which has to be removed from protein preparations before medical use [8]. In contrast to Bacillus subtilis, L. lactis secretes only one major endogenous protein, Usp45, and no proteases [9]: a strategy combining production and secretion of heterologous proteins in L. lactis is thus interesting as it facilitates protein purification from the culture medium [4]. As secreted heterologous proteins can be degraded by the lactococcal surface protease HtrA, protein yield can be improved by the use of a mutant strain devoid of ...
Difference between revisions of IGEM:Caltech/2008/Project/Vitamins - OpenWetWareDifference between revisions of "IGEM:Caltech/2008/Project/Vitamins" - OpenWetWare
Although folate is naturally produced in E.coli, the folate biosynthesis pathway in the bacteria Lactococcus lactis has been more heavily characterized and studied. There are six major enzymes involved in folate synthesis, which, in L.lactis, are contained in five genes: folB, folKE, folP, folC, and folA[1]. The first four, which we have chosen to focus on, are located in a gene cluster approximately 4.4kb long. Weve chosen not to focus on folA for the time being because folA encodes an enzyme which turns one form of folate (dihydrofolate) into another form of folate(tetrahydrofolate). Since our assay would detect both types of folate as part of the total folate production, folA was not a prime target for overexpression of folate. In previous studies, this folate gene cluster has been successfully transformed into the folate-consuming bacteria L.gasseri, turning the bacteria in to folate-producers[3]. Therefore, we have chosen to also use the folate operon from L.lactis, which also offers the ...
Difference between revisions of IGEM:Caltech/2008/Project/Vitamins - OpenWetWareDifference between revisions of "IGEM:Caltech/2008/Project/Vitamins" - OpenWetWare
Although folate is naturally produced in E.coli, the folate biosynthesis pathway in the bacteria Lactococcus lactis has been more heavily characterized and studied. There are six major enzymes involved in folate synthesis, which, in L.lactis, are contained in five genes: folB, folKE, folP, folC, and folA[1]. The first four, which we have chosen to focus on, are located in a gene cluster approximately 4.4kb long. Weve chosen not to focus on folA for the time being because folA encodes an enzyme which turns one form of folate (dihydrofolate) into another form of folate(tetrahydrofolate). Since our assay would detect both types of folate as part of the total folate production, folA was not a prime target for overexpression of folate. In previous studies, this folate gene cluster has been successfully transformed into the folate-consuming bacteria L.gasseri, turning the bacteria in to folate-producers[3]. Therefore, we have chosen to also use the folate operon from L.lactis, which also offers the ...
LactococcusLactococcus
How was I to know?. Lactococcus lactis is part of my "culture and heritage". Does that make me uncultured for not knowing about this cultures importance, or, to be honest, not knowing about it at all? L.lactis is the microbe which changes milk into many varieties of cheese.. The Assembly in my fair state, Wisconsin, has passed a bill designating Lactococcus lactis as our official state microbe. Since our State Senate has yet to vote, Lactococcus is lobbying hard. How many other bacterium have their own home pages? (The Lactococcus lactis Home Page). At the risk of sounding like a disloyal cheesehead, I am a bit upset about the designation. The fact that my state has been made a laughing stock by the media doesnt faze me. Living in a place where people don large wedges of foam cheese on their heads, I dont fret much about the states image.. No, I am upset because the candidate that I am backing for state designation is languishing in some committee over in Madison. How can our state go " ...
Forzea - Probiotic Night CreamForzea - Probiotic Night Cream
Our Probiotic Night Cream is virtually a miracle treatment for damaged skin and has several high-end ingredients that have been developed over years of testing and research. What is Probiotic Technology? Probiotics are bacteria that are in some form beneficial to us. Our Probiotic Night Cream contains a biotechnologically obtained suspension from the bacterium Lactococcus lactis that contains cytoplasm and cell wall fragments which are highly effective in helping the skin to heal and to remain healthy and younger appearing. Some benefits of our Probiotic Technology are: • Induces production of key ingredients of skin quality and differentiation resulting in healthier skin. • Improves keratinocyte cohesion resulting in a more robust skin barrier that protects the skin. • Increases the production of antimicrobial peptides which fight harmful bacteria. • Promotes skin renewal (old cells being replaced with new and healthier cells). In addition to our Probiotic Technology, weve also added ...
IAR Gandhinagar Vaccine Development JRF VacancyIAR Gandhinagar Vaccine Development JRF Vacancy
Applications are invited for the position of Junior Research Fellow on SERB sponsored project entitled "Evaluation of immunogenicity and protective efficacy of bivalent vaccine candidate against Shigella and Salmonella using food grade bacteria Lactococcus lactis" under the supervision of Dr. Priti Desai, Institute of Advanced Research, Koba Institutional Area, Koba, Gandhinagar-382 007. ...
ldhX - L-lactate dehydrogenase 3 - Lactococcus lactis subsp. lactis (strain IL1403) - ldhX gene & proteinldhX - L-lactate dehydrogenase 3 - Lactococcus lactis subsp. lactis (strain IL1403) - ldhX gene & protein
p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.,/p> ,p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.,/p> ,p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).,/p> ,p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x,sup>64,/sup> + x,sup>4,/sup> + x,sup>3,/sup> + x + 1. The algorithm is described in the ISO 3309 standard. ,/p> ,p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.,br /> ,strong>Cyclic redundancy and other checksums,/strong>,br /> ,a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993),/a>),/p> Checksum:i ...
KEGG PATHWAY: Ubiquinone and other terpenoid-quinone biosynthesis - Lactococcus lactis subsp. cremoris SK11KEGG PATHWAY: Ubiquinone and other terpenoid-quinone biosynthesis - Lactococcus lactis subsp. cremoris SK11
Ubiquinone (UQ), also called coenzyme Q, and plastoquinone (PQ) are electron carriers in oxidative phosphorylation and photosynthesis, respectively. The quinoid nucleus of ubiquinone is derived from the shikimate pathway; 4-hydroxybenzoate is directly formed from chorismate in bacteria, while it can be formed from either chorismate or tyrosine in yeast. The following biosynthesis of terpenoid moiety involves reactions of prenylation, decarboxylation, and three hydroxylations alternating with three methylations. The order of these reactions are somewhat different between bacteria and yeast. Phylloquinone (vitamin K1), menaquinone (vitamin K2), and tocopherol (vitamin E) are fat-soluble vitamins. Phylloquinone is a compound present in all photosynthetic plants serving as a cofactor for photosystem I-mediated electron transport. Menaquinone is an obligatory component of the electron-transfer pathway in bacteria ...
Overall control of nitrogen metabolism in Lactococcus lactis by CodY, and possible models for CodY regulation in FirmicutesOverall control of nitrogen metabolism in Lactococcus lactis by CodY, and possible models for CodY regulation in Firmicutes
CodY, a pleiotropic transcriptional regulator conserved in low G+C species of Gram-positive bacteria, was previously described to be the central regulator of proteolysis in Lactococcus lactis. In this study, over 100 potential CodY targets were identified by DNA-microarray analysis. Complementary tr …
A Genome-Scale Integration and Analysis of Lactococcus lactis Translation Data - InfoscienceA Genome-Scale Integration and Analysis of Lactococcus lactis Translation Data - Infoscience
Protein synthesis is a template polymerization process composed by three main steps: initiation, elongation, and termination. During translation, ribosomes are engaged into polysomes whose size is used for the quantitative characterization of translatome. However, simultaneous transcription and translation in the bacterial cytosol complicates the analysis of translatome data. We established a procedure for robust estimation of the ribosomal density in hundreds of genes from Lactococcus lactis polysome size measurements. We used a mechanistic model of translation to integrate the information about the ribosomal density and for the first time we estimated the protein synthesis rate for each gene and identified the rate limiting steps. Contrary to conventional considerations, we find significant number of genes to be elongation limited. This number increases during stress conditions compared to optimal growth and proteins synthesized at maximum rate are predominantly elongation limited. Consistent with
Antibody responses to two new Lactococcus lactis -produced recombinant Pfs48/45 and Pfs230 proteins increase with age in...Antibody responses to two new Lactococcus lactis -produced recombinant Pfs48/45 and Pfs230 proteins increase with age in...
Recent advances in malaria control efforts have led to an increased number of national malaria control programmes implementing pre-elimination measures and demonstrated the need to develop new tools to track and control malaria transmission. Key to understanding transmission is monitoring the prevalence and immune response against the sexual stages of the parasite, known as gametocytes, which are responsible for transmission. Sexual-stage specific antigens, Pfs230 and Pfs48/45, have been identified and shown to be targets for transmission blocking antibodies, but they have been difficult to produce recombinantly in the absence of a fusion partner. Regions of Pfs48/45 and Pfs230 known to contain transmission blocking epitopes, 6C and C0, respectively, were produced in a Lactococcus lactis expression system and used in enzyme linked immunosorbent assays to determine the seroreactivity of 95 malaria patients living in the Central Region of Ghana. Pfs48/45.6C and Pfs230.C0 were successfully produced in L.
Lactococcus Lactis Probiotics - Lyophilized Probiotics SupplementsLactococcus Lactis Probiotics - Lyophilized Probiotics Supplements
Get The Natural Lactococcus Lactis Probiotics Products Online From DBI360.com. We Provide The Best Lyophilized Vitamin Products & Probiotics Ingredients As Per Your Requirement.
Lactococcus lactis - WikipediaLactococcus lactis - Wikipedia
L. lactis is of crucial importance for manufacturing dairy products, such as buttermilk and cheeses. When L. lactis ssp. lactis is added to milk, the bacterium uses enzymes to produce energy molecules (ATP), from lactose. The byproduct of ATP energy production is lactic acid. The lactic acid produced by the bacterium curdles the milk that then separates to form curds, which are used to produce cheese.[11] Other uses that have been reported for this bacterium include the production of pickled vegetables, beer or wine, some breads, and other fermented foodstuffs, such as soymilk kefir, buttermilk, and others.[12] L. lactis is one of the best characterized low GC Gram positive bacteria with detailed knowledge on genetics, metabolism and biodiversity.[13][14]. L. lactis is mainly isolated from either the dairy environment or plant material.[15][16][17] Dairy isolates are suggested to have evolved from plant isolates through a process in which genes without benefit in the rich medium milk were either ...
Citrate Metabolism - Beverage Fermentation - Buffalo Brewing BlogCitrate Metabolism - Beverage Fermentation - Buffalo Brewing Blog
Citrate is present in only low levels 0.15-0.2 in milk and O-type starters do not include the citrate utilizing Lc. lactis subsp. lactis biovar diacetylactis.
xseB - Exodeoxyribonuclease 7 small subunit - Lactococcus lactis subsp. cremoris (strain SK11) - xseB gene & proteinxseB - Exodeoxyribonuclease 7 small subunit - Lactococcus lactis subsp. cremoris (strain SK11) - xseB gene & protein
Bidirectionally degrades single-stranded DNA into large acid-insoluble oligonucleotides, which are then degraded further into small acid-soluble oligonucleotides.
Staff PublicationsStaff Publications
Background: Vitamin K2 (menaquinone, MK-n) is a lipid-soluble vitamin that functions as a carboxylase co-factor for maturation of proteins involved in many vital physiological processes in humans. Notably, long-chain vitamin K2 is produced by bacteria, including some species and strains belonging to the group of lactic acid bacteria (LAB) that play important roles in food fermentation processes. This study was performed to gain insights into the natural long-chain vitamin K2 production capacity of LAB and the factors influencing vitamin K2 production during cultivation, providing a basis for biotechnological production of vitamin K2 and in situ fortification of this vitamin in food products. Results: We observed that six selected Lactococcus lactis strains produced MK-5 to MK-10, with MK-8 and MK-9 as the major MK variant. Significant diversities between strains were observed in terms of specific concentrations and titres of vitamin K2. L. lactis ssp. cremoris MG1363 was selected for more ...
Systems solutions by lactic acid bacteria: from paradigms to practice | Microbial Cell Factories | Full TextSystems solutions by lactic acid bacteria: from paradigms to practice | Microbial Cell Factories | Full Text
One of the great achievements of the omics revolution is the development of high throughput functional genomics approaches. Notably transcriptomics studies have been instrumental in analyzing the response of LAB to different environments and over 100 papers have been published addressing a variety of stresses, growth conditions and culturing regimens. Among the most important practical discoveries was the finding that Lactococcus lactis and other LAB, when provided with the appropriate cofactors, such as heme, could use alternative electron acceptors and hence respire rather than ferment [82]. Following the first description of the use of molecular oxygen by Lactococcus lactis MG1363 [83], a variety of studies have followed that have been reviewed recently [84]. Transcriptional studies allowed to identify the genes involved in the use of molecular oxygen that resulted in a wide range of applications, notably faster growth and higher yield of starter cultures [85]. Biochemical studies confirmed ...
Lactococcus lactis (Streptococcus lactis) - CTValley BioLactococcus lactis (Streptococcus lactis) - CTValley Bio
Gram-positive sphere. Forms short chains. A common contaminant of milk products, causing souring. Excellent for classroom demonstrations. Incubate at 37°C. On brain heart infusion …
Viruses | Free Full-Text | Structural Aspects of the Interaction of Dairy Phages with Their Host BacteriaViruses | Free Full-Text | Structural Aspects of the Interaction of Dairy Phages with Their Host Bacteria
Knowledge of phage-host interactions at a fundamental level is central to the design of rational strategies for the development of phage-resistant strains that may be applied in industrial settings. Phages infecting lactic acid bacteria, in particular Lactococcus lactis and Streptococcus thermophilus, negatively impact on dairy fermentation processes with serious economic implications. In recent years a wealth of information on structural protein assembly and topology has become available relating to phages infecting Escherichia coli, Bacillus subtilis and Lactococcus lactis, which act as models for structural analyses of dairy phages. In this review, we explore the role of model tailed phages, such as T4 and SPP1, in advancing our knowledge regarding interactions between dairy phages and their hosts. Furthermore, the potential of currently investigated dairy phages to in turn serve as model systems for this particular group of phages is discussed.
Viruses | Free Full-Text | Structural Aspects of the Interaction of Dairy Phages with Their Host Bacteria | HTMLViruses | Free Full-Text | Structural Aspects of the Interaction of Dairy Phages with Their Host Bacteria | HTML
Knowledge of phage-host interactions at a fundamental level is central to the design of rational strategies for the development of phage-resistant strains that may be applied in industrial settings. Phages infecting lactic acid bacteria, in particular Lactococcus lactis and Streptococcus thermophilus, negatively impact on dairy fermentation processes with serious economic implications. In recent years a wealth of information on structural protein assembly and topology has become available relating to phages infecting Escherichia coli, Bacillus subtilis and Lactococcus lactis, which act as models for structural analyses of dairy phages. In this review, we explore the role of model tailed phages, such as T4 and SPP1, in advancing our knowledge regarding interactions between dairy phages and their hosts. Furthermore, the potential of currently investigated dairy phages to in turn serve as model systems for this particular group of phages is discussed.
SWISS-MODEL Repository | P96790SWISS-MODEL Repository | P96790
SWISS-MODEL Repository entry for P96790 (KUP1_LACLM), Putative potassium transport system protein kup 1. Lactococcus lactis subsp cremoris (strain MG1363)
SWISS-MODEL Repository | A2RNR2SWISS-MODEL Repository | A2RNR2
SWISS-MODEL Repository entry for A2RNR2 (RL333_LACLM), 50S ribosomal protein L33 3. Lactococcus lactis subsp cremoris (strain MG1363)
Functions of starters in dairy fermentations. The importance and effectiveness of their antimicrobial mechanismsFunctions of starters in dairy fermentations. The importance and effectiveness of their antimicrobial mechanisms
The functions of starters in dairy fermentations and the importance of the antimicrobial agents produced by starters including undissociated lactic acid is discussed
Curr Microbiol 68(2), 254-261, 2014 Publication Passport - StrainInfoCurr Microbiol 68(2), 254-261, 2014 Publication Passport - StrainInfo
Lactococcus lactis subsp. lactis strain Il1403 16S ribosomal RNA gene, partial sequence; tRNA-Ala gene, complete sequence; and 23S ribosomal RNA gene, partial ...
Sensing the State of the Membrane | Science SignalingSensing the State of the Membrane | Science Signaling
The ability to osmoregulate is essential to survival. Van der Heide and Poolman cloned and reconstituted into liposomes the purified components of the osmoregulated ABC transporter (OpuA) from Lactococcus lactis. With an ATP-regenerating system inside the liposomes, the OpuA system was able to transport glycine betaine into the liposomes. The uptake was stimulated by a transmembrane osmotic gradient of either ionic or nonionic solutes. Furthermore, transport activity was stimulated by tetracaine, an amphiphile that alters membrane curvature, mimicking the membrane stress that occurs under hyperosmotic conditions. The data suggest that the two proteins of the OpuA system serve as both the osomosensor and the osmoregulator and that the osmotic gradient is sensed as changes in the membrane bilayer.. van der Heide, T., and Poolman, B. (2000) Osmoregulated ABC-transport system of Lactococcus lactis senses water stress via changes in the physical state of the membrane. Proc. Natl. Acad. Sci. U.S.A. ...
Lactococcus lactisLactococcus lactis
Disclaimer:. The information contained on this web site is provided for your general information only. SuperSizeHealth.com.com does not give medical advice or engage in the practice of medicine. SuperSizeHealth.com.com under no circumstances recommends particular treatment for specific individuals and in all cases recommend that you consult your physician or local treatment center before pursuing any course of treatment.. Please remember that this information is not meant to substitute for a consultation with your physician, or another health care professional. Speak with your doctor if you have questions about primary care, or about any medical problem.. These statements have not been evaluated by the Food and Drug Administration. These products are not intended to diagnose, treat, cure, or prevent any disease, but rather are dietary supplements intended solely for nutritional use.. ...
Kitahara 117 Strain Passport - StrainInfoKitahara 117 Strain Passport - StrainInfo
Lactococcus lactis subsp. lactis (Lactobacillus xylosus) strain NRRL B-4449xylose regulatory protein (xylR), xylose isomerase (xylA), xylulokinase(xylB), mutarotase (xylM), and xyloside transporter (xynT) genes, completecds; and beta-1,4-xylosidase (xynB) gene, partial ...
Phages: BacteriaPhages: Bacteria
Saxelin, M.-L., E.-L. Nurmiaho-Lassila, V. T. Meril�inen, and R. I. Fors�n. 1986. Ultrastructure and Host Specificity of Bacteriophages of Streptococcus cremoris, Streptococcus lactis subsp. diacetylactis, and Leuconostoc cremoris from Finnish Fermented Milk ?Viili?. Appl. Environ. Microbiol. 52:771-777. ...
Objave » Inštitut za mlekarstvo in probiotikeObjave » Inštitut za mlekarstvo in probiotike
SAMELIS, John, LIANOU, Alexandra, PAPPA, Eleni C., BOGOVIČ MATIJAŠIĆ, Bojana, PARAPOULI, Maria, KAKOURI, Athanasia, ROGELJ, Irena. Behavior of Staphylococcus aureus in culture broth, in raw and thermized milk, and during processing and storage of traditional Greek Graviera cheese in the presence or absence of Lactococcus lactis subsp. cremoris M104, a wild, novel nisin A-producing raw milk isolate. Journal of food protection, 2014, vol. 77, no. 10, str. 1703-1714. doi:10.4315/0362-028X.JFP-14-105 ...
Thymidylate synthase gene from Lactococcus lactis as a genetic marker: an alternative to antibiotic resistance genes. | Applied...Thymidylate synthase gene from Lactococcus lactis as a genetic marker: an alternative to antibiotic resistance genes. | Applied...
The potential of the thymidylate synthase thyA gene cloned from Lactococcus lactis subsp. lactis as a possible alternative selectable marker gene to antibiotic resistance markers has been examined. The thyA mutation is a recessive lethal one; thyA mutants cannot survive in environments containing low amounts of thymidine or thymine (such as Luria-Bertani medium) unless complemented by the thyA gene. The cloned thyA gene was strongly expressed in L. lactis subsp. lactis, Escherichia coli, Rhizobium meliloti, and a fluorescent Pseudomonas strain. In addition, when fused to a promoterless enteric lac operon, the thyA gene drove expression of the lac genes in a number of gram-negative bacteria. In transformation experiments with thyA mutants of E. coli and conjugation experiments with thyA mutants of R. meliloti, the lactococcal thyA gene permitted selection of transformants and transconjugants with the same efficiency as did genes for resistance to ampicillin, chloramphenicol, or tetracycline. ...
Nisin - definition of nisin by The Free DictionaryNisin - definition of nisin by The Free Dictionary
Define nisin. nisin synonyms, nisin pronunciation, nisin translation, English dictionary definition of nisin. n. A bacteriocin produced by the bacterium Lactococcus lactis and used as a preservative in dairy products and other foods and in cosmetics
Structure, Adsorption to Host, and Infection Mechanism of Virulent Lactococcal Phage p2 | Journal of VirologyStructure, Adsorption to Host, and Infection Mechanism of Virulent Lactococcal Phage p2 | Journal of Virology
We report here the complete EM structure of a lactococcal phage determined by single-particle analysis. Phage p2 is the flagship of the 936 group, which are the most prevalent lactococcal phages in industrial dairy fermentations worldwide. This phage is highly virulent and requires Ca2+ to infect specific L. lactis cells, which is in contrast with the less prevalent lactococcal phages belonging to the P335 subgroup II (e.g., TP901-1 and Tuc2009) (15).. The shape of p2 icosahedral capsid is comparable to that of other siphophages. The capsid pentons are, however, more prominent than those of phage TP901-1 but akin to those of coliphage HK97. In contrast, the size of the p2 portal protein (ORF-4) is among the smallest, particularly in comparison to those of phage TP901-1 and Bacillus phage SPP1 (Table 3). The two putative head completion proteins of p2 (ORF-8 and ORF-9) are comparable to those of phage SPP1. The fit of the SPP1 portal and head completion protein 1 (gp15) into the p2 connector EM ...
Control analysis as a tool to understand the formation of the las operon in Lactococcus lactis - DTU OrbitControl analysis as a tool to understand the formation of the las operon in Lactococcus lactis - DTU Orbit
In Lactococcus lactis the enzymes phosphofructokinase (PFK), pyruvate kinase (PK) and lactate dehydrogenase (LDH) are uniquely encoded in the las operon and we here apply Metabolic Control Analysis to study the role of this organisation. Earlier work showed that LDH at wildtype level has zero control on glycolysis and growth rate but high negative control on formate production. We find that PFK and PK have zero control on glycolysis and growth rate at the wildtype enzyme level but both enzymes exert strong positive control on the glycolytic flux at reduced activities. PK has high positive control on formate and acetate production, whereas PFK has no control on these fluxes. Decreased expression of the entire las operon resulted in a strong decrease in growth rate and the glycolytic flux; at 53% expression of the las operon the glycolytic flux was reduced to 44% and the flux control coefficient increased towards 3. Increased las expression resulted in a slight decrease in the glycolytic flux. At ...
Production of Human Papillomavirus Type 16 E7 Protein in Lactococcus lactis | Applied and Environmental MicrobiologyProduction of Human Papillomavirus Type 16 E7 Protein in Lactococcus lactis | Applied and Environmental Microbiology
The E7 protein of human papillomavirus type 16 was produced in Lactococcus lactis. Secretion allowed higher production yields than cytoplasmic production. In stationary phase, amounts of cytoplasmic E7 were reduced, while amounts of secreted E7 increased, suggesting a phase-dependent intracellular proteolysis. Fusion of E7 to the staphylococcal nuclease, a stable protein, resulted in a highly stable cytoplasmic protein. This work provides new candidates for development of viral screening systems and for oral vaccine against cervical cancer. ...
Probiotičke kulture Lactobacillus plantarum B i Lactococcus lactis subsp. lactis S1 u poboljšanju funkcionalnih svojstava sira...Probiotičke kulture Lactobacillus plantarum B i Lactococcus lactis subsp. lactis S1 u poboljšanju funkcionalnih svojstava sira...
Cheese in a sack is a specific Croatian indigenous semi hard cheese. The main characteristic of this cheese is ripening and keeping in sheeps clothing (Mišina), which gives it a typical and very appreciated, spicy flavor and aroma. Cheeses obtained by similar technology and ripening are also produced in Turkey (Tulum cheese), Lebanon (darfiyeh), Bosnia and Herzegovina (sack cheese), Montenegro (sack cheese) and eastern Algeria (bouhezza cheese). In Croatia, cheese in a sack has been produced traditionally for many years, without the use of commercial starter cultures, which can result in differences in taste, consistency and microbiological quality.Therefore, there is a growing trend of finding and selecting of new natural indigenous potential starter cultures from traditional products, which will be adapted to the growth in certain specific traditional products, and will have such metabolic capabilities that will significantly affect the quality and safety of products. The hypothesis is that ...
Protein balance of Lactococcus lactis in cheese production - WURProtein balance of Lactococcus lactis in cheese production - WUR
Lactic acid bacteria are used as starter cultures in dairy fermentations. Starter cultures employed in cheese manufacturing encounter many different environmental conditions during their production in starter-producing companies and during their application in the cheese manufacturing industry. To optimally perform, microorganisms have to continuously adapt to these changing conditions. Bacteria adjust their physiology to maximize their fitness to the condition they are in, at the cost of being less fit under different conditions. Microbial fitness under different conditions is determined by rate of adaptation including proteome degradation, production and renewal. Adaptation and turnover of the proteome is constrained by the availability of resources and/or cellular energy levels, which results in trade-offs during optimized growth followed by adaptation: the optimization of one trait usually correlates with decreased performance of another trait. In this project, we investigate, by proteomics, ...
Get PDF - Proteolytic action of lactic streptococci. I. The action of mesophilic lactic streptococci on N constituents of milkGet PDF - Proteolytic action of lactic streptococci. I. The action of mesophilic lactic streptococci on N constituents of milk
Kikuchi, T.; Desmazeaud, M.; Bergere, J.L., 1973: Proteolytic action of lactic streptococci. I. The action of mesophilic lactic streptococci on N constituents of milk
Bifidobacterium lactis - NutriveeBifidobacterium lactis - Nutrivee
B. lactis is a widely used probiotic and is considered a champion among the "good bacteria". It has been reclassified by Masco et al. (2004) and has been found to be related to Bifidobacterium animalis at the sup specie level, B. animalis subsp. Lactis. B. lactis is a common inhabitant of the colon and intestines of humans and animals. The use of B. lactis in diets has shown beneficial effects in the treatment of various gluten-based enteropathies. Celiac disease is an autoimmune disorder which is aggravated by the consumption of gluten-based food products.. According to Lindfors et al. (2008), the oral intake of B. lactis based-probiotics protects the intestinal cells from the toxic effects of gluten enteropathies and inflammatory reactions. The regular consumption of B. lactis aids in controlling abnormal weight gain and diabetes in animal models. Stenman et al. (2014) administered a probiotic based on Bifidobacterium animalis ssp. Lactis 420 to mice, which showed positive results in ...
Disruption and Analysis of the clpB, clpC, and clpE Genes in Lactococcus lactis: ClpE, a New Clp Family in Gram-Positive...Disruption and Analysis of the clpB, clpC, and clpE Genes in Lactococcus lactis: ClpE, a New Clp Family in Gram-Positive...
PubMed Central Canada (PMC Canada) provides free access to a stable and permanent online digital archive of full-text, peer-reviewed health and life sciences research publications. It builds on PubMed Central (PMC), the U.S. National Institutes of Health (NIH) free digital archive of biomedical and life sciences journal literature and is a member of the broader PMC International (PMCI) network of e-repositories.
EC 2.4.1.216EC 2.4.1.216
Accepted name: trehalose 6-phosphate phosphorylase. Reaction: α,α-trehalose 6-phosphate + phosphate = glucose 6-phosphate + β-D-glucose 1-phosphate. Systematic name: α,α-trehalose 6-phosphate:phosphate β-D-glucosyltransferase. Comments: The enzyme from Lactococcus lactis is specific for trehalose 6-phosphate. Differs from EC 2.4.1.64, α,α-trehalose phosphorylase, in that trehalose is not a substrate.. Links to other databases: BRENDA, EXPASY, KEGG, Metacyc, CAS registry number: 403512-51-6. References:. 1. Andersson, U., Levander, F. and Radstrom, P. Trehalose 6-phosphate phosphorylase is part of a novel metabolic pathway for trehalose utilization in Lactococcus lactis. J. Biol. Chem. 276 (2001) 42707-42713. [PMID: 11553642]. ...
Antibody responses to two new Lactococcus lactis-produced recombinant Pfs48/45 and Pfs230 proteins increase with age in malaria...Antibody responses to two new Lactococcus lactis-produced recombinant Pfs48/45 and Pfs230 proteins increase with age in malaria...
Recent advances in malaria control efforts have led to an increased number of national malaria control programmes implementing pre-elimination measures and demonstrated the need to develop new tools to track and control malaria transmission. Key to understanding transmission is monitoring the prevalence and immune response against the sexual stages of the parasite, known as gametocytes, which are responsible for transmission. Sexual-stage specific antigens, Pfs230 and Pfs48/45, have been identified and shown to be targets for transmission blocking antibodies, but they have been difficult to produce recombinantly in the absence of a fusion partner. Regions of Pfs48/45 and Pfs230 known to contain transmission blocking epitopes, 6C and C0, respectively, were produced in a Lactococcus lactis expression system and used in enzyme linked immunosorbent assays to determine the seroreactivity of 95 malaria patients living in the Central Region of Ghana. Pfs48/45.6C and Pfs230.C0 were successfully produced in L.
Enhanced Nutty Flavor Formation in Cheddar Cheese Made with Malty Lact by M. E. Carunchia Whetstine, M. A. Drake et al."Enhanced Nutty Flavor Formation in Cheddar Cheese Made with Malty Lact" by M. E. Carunchia Whetstine, M. A. Drake et al.
Carunchia Whetstine, M. E.; M. A. Drake, J. R Broadbent, and McMahon. 2006. Enhanced nutty flavor formation in Cheddar cheese made with a malty Lactococcus lactis adjunct culture. J. Dairy Sci. 89: 3277-3284. ...
prof.dr. M. (Michiel) Kleerebezem - WURprof.dr. M. (Michiel) Kleerebezem - WUR
His expertise centers around the molecular biology and physiology of bacteria, with a special focus on lactic acid bacteria, probiotics, and the intestinal microbiota. He is strongly involved in the genomics driven work related to understanding the molecular mechanisms of environmental adaptation in Lactobacillus plantarum and Lactococcus lactis, focusing on adaptation to the conditions encountered in the oro-gastrointestinal tract and conditions encountered during (industrial) dairy fermentation, respectively. In recent years he has expanded his field to the (post-genomic) molecular analysis of the mechanisms of communication between intestinal bacteria and the host mucosa. Michiel Kleerebezem has (co-) authored more than 200 peer-reviewed publications and is inventor on more than 25 patent applications ...
Genomic Characterization of Dairy Associated Leuconostoc Species and D by Cyril A. Frantzen, Witold Kot et al."Genomic Characterization of Dairy Associated Leuconostoc Species and D" by Cyril A. Frantzen, Witold Kot et al.
Undefined mesophilic mixed (DL-type) starter cultures are composed of predominantly Lactococcus lactis subspecies and 1-10% Leuconostoc spp. The composition of the Leuconostoc population in the starter culture ultimately affects the characteristics and the quality of the final product. The scientific basis for the taxonomy of dairy relevant leuconostocs can be traced back 50 years, and no documentation on the genomic diversity of leuconostocs in starter cultures exists. We present data on the Leuconostoc population in five DL-type starter cultures commonly used by the dairy industry. The analyses were performed using traditional cultivation methods, and further augmented by next-generation DNA sequencing methods. Bacterial counts for starter cultures cultivated on two different media, MRS and MPCA, revealed large differences in the relative abundance of leuconostocs. Most of the leuconostocs in two of the starter cultures were unable to grow on MRS, emphasizing the limitations of culture-based methods
Munin: Bacterial diversity of aMasi, a South African fermented milk product, determined by clone library and denaturing...Munin: Bacterial diversity of aMasi, a South African fermented milk product, determined by clone library and denaturing...
In the present study, we investigated the bacterial diversity of aMasi, a traditional South African fermented milk product, by 16S rRNA clone library and Denaturing Gradient Gel Electrophoresis (DGGE) analysis. Two hundred and eighty two clones from clone library were isolated and identified from aMasi, prepared from the milk of four cows from one herd in the EkuPindiseni Community, North West of Hluhluwe-iMfolozi Park in KwaZulu-Natal Province. The majority of the identified sequences corresponded to lactic acid bacteria (LAB), with the genus Lactococcus as major representative. The species Lactococcus lactis accounted for 179 of the identified clones. In addition, several species of Lactobacillus, Leuconostoc and Enterococcus were detected. Furthermore, several clones belonging to Acinetobacter, Aeromonas and genera within the Enterobacteriaceae were detected. It is important to note that human pathogens such as Klebsiella pneumoniae were identified in aMasi in the present study. Conversely, ...
Cross-immunity and immune mimicry as mechanisms of resistance to the lantibiotic lacticin 3147. - PubMed - NCBICross-immunity and immune mimicry as mechanisms of resistance to the lantibiotic lacticin 3147. - PubMed - NCBI
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Characterization of the lactococcal conjugative element pRS01 using IS946-mediated mutagenesis | SpringerLinkCharacterization of the lactococcal conjugative element pRS01 using IS946-mediated mutagenesis | SpringerLink
Conjugation is a common mode of genetic transfer among the lactic acid bacteria. In an effort to exploit conjugation as a means of lactococcal strain development, we have characterized the transfer re
Get PDF - Production of proteolytic activity by selected strains of lactic streptococci in skim milk and a whey mediumGet PDF - Production of proteolytic activity by selected strains of lactic streptococci in skim milk and a whey medium
Vahora, G.Y.; Ernstrom, C.A.; Post, F.J., 1973: Production of proteolytic activity by selected strains of lactic streptococci in skim milk and a whey medium
Variability at gene and genome levels revealed different genomic lineages in Lactococcus garvieae | Archivio Istituzionale...Variability at gene and genome levels revealed different genomic lineages in Lactococcus garvieae | Archivio Istituzionale...
Lactococcus garvieae is a pathogen that causes septicemia in fish and serious damage to fish aquaculture worldwide, but recently has been isolated from human, ruminant, water sources and several food matrix. In the present work we monitored the population structure of L. garvieae strains, including isolates of dairy, fish, meat, vegetable and cereal origin. This bacterial collection was explored using molecular polyphasic approach comprising PCR-ribotyping, Rep and RAPD-PCR analyses and multilocus restriction typing (MLRT) carried out on six partial genes. This approach allowed high-resolution cluster analysis in which two major groups were distinguishable: one group included dairy isolates, the other group meat isolates. Unexpectedly, some strains coming from fish grouped with dairy isolates whereas the other with meat isolates. Likewise, strains isolated from vegetables allocated between the two main groups. These findings revealed high variability within the species at both gene and genome ...
Structure and Conformational Dynamics of the Domain 5 RNA Hairpin of a Bacterial Group II Intron Revealed by Solution Nuclear...Structure and Conformational Dynamics of the Domain 5 RNA Hairpin of a Bacterial Group II Intron Revealed by Solution Nuclear...
Structure and Conformational Dynamics of the Domain 5 RNA Hairpin of a Bacterial Group II Intron Revealed by Solution Nuclear Magnetic Resonance and Molecular Dynamics ...
Diamond Publications - Search ResultsDiamond Publications - Search Results
Abstract: Multidrug efflux pumps present a challenge to the treatment of bacterial infections, making it vitally important to understand their mechanism of action. Here, we investigate the nature of substrate binding within Lactococcus lactis LmrP, a prototypical multidrug transporter of the major facilitator superfamily. We determined the crystal structure of LmrP in a ligand-bound outward-open state and observed an embedded lipid in the binding cavity of LmrP, an observation supported by native mass spectrometry analyses. Molecular dynamics simulations suggest that the anionic lipid stabilizes the observed ligand-bound structure. Mutants engineered to disrupt binding of the embedded lipid display reduced transport of some, but not all, antibiotic substrates. Our results suggest that a lipid within the binding cavity could provide a malleable hydrophobic component that allows adaptation to the presence of different substrates, helping to explain the broad specificity of this protein and ...
The FAIRDOMHubThe FAIRDOMHub
Im a modeller, specialized in kinetic modeling of biochemical networks. My focus in the SysMO-LAB consortium is on creating models of Lactococcus lactis glycolysis and couple this to other related lactic acid bacteria like Streptococcus pyogenes and Enterococcus faecalis. Besides kinetic modeling, Im also interested in combining various modeling techniques (genome-scale modeling, qualitative modeling). ...
The FAIRDOMHubThe FAIRDOMHub
Im a modeller, specialized in kinetic modeling of biochemical networks. My focus in the SysMO-LAB consortium is on creating models of Lactococcus lactis glycolysis and couple this to other related lactic acid bacteria like Streptococcus pyogenes and Enterococcus faecalis. Besides kinetic modeling, Im also interested in combining various modeling techniques (genome-scale modeling, qualitative modeling). ...
Artificial Promoters for Metabolic Optimization - DTU OrbitArtificial Promoters for Metabolic Optimization - DTU Orbit
In this article, we review some of the expression systems that are available for Metabolic Control Analysis and Metabolic Engineering, and examine their advantages and disadvantages in different contexts. In a recent approach, artificial promoters for modulating gene expression in micro-organisms were constructed using synthetic degenerated oligonucleotides. From this work, a promoter library was obtained for Lactococcus lactis, containing numerous individual promoters and covering a wide range of promoter activities. Importantly, the range of promoter activities was covered in small steps of activity change. Promoter libraries generated by this approach allow for optimization of gene expression and for experimental control analysis in a wide range of biological systems by choosing from the promoter library promoters giving, e.g., 25%, 50%, 200%, and 400% of the normal expression level of the gene in question. If the relevant variable (e.g., the flux or yield) is then measured with each of these ...
Articles - LISBP Laboratoire dIngénierie des Systèmes Biologiques et des ProcédésArticles - LISBP Laboratoire d'Ingénierie des Systèmes Biologiques et des Procédés
Vermassen A, Dordet-Frisoni E, de LaFoye A, Micheau P, Laroute V, Leroy S, Talon R. 2016. Adaptation of Staphylococcus xylosus to nutrients and osmotic stress in a salted meat model. Front Microbiol. Feb 7;87.. Morin M, Ropers D, Letisse F, Laguerre S, Portais J-C, Enjalbert B, Cocaign-Bousquet M. 2016. The post-transcriptional regulatory system CSR controls the balance of metabolic pools in upper glycolysis of Escherichia coli. Mol Microbiol ; doi: 10.1111/mmi.13343. Castelain M., Duviau M-P, Canette A, Schmitz P, Loubière P, Cocaign-Bousquet M, Piard J-C, Mercier-Bonin M. 2016. The nanomechanical properties of Lactococcus lactis pili are conditioned by the polymerized backbone pilin. PLoS ONE 11(3): e0152053. doi:10.1371/journal.pone.0152053. Esquerré T, Turlan C, Bouvier M, Carpousis AJ, Girbal L, Cocaign-Bousquet M. 2016. The Csr system regulates genome-wide mRNA stability and transcription and thus gene expression in Escherichia coli. Sci Rep. 2016 Apr 26;6:25057. doi: ...
TCDB » SEARCHTCDB » SEARCH
2.A.105 The Mitochondrial Pyruvate Carrier (MPC) Family. The transport of pyruvate, the end product of glycolysis, into mitochondria is an essential process that provides the organelle with a major oxidative fuel. Herzig et al. (2012) reported that MPC is a heterocomplex formed by two members of the MPC family that are conserved from yeast to mammals. Members of the MPC family are in the inner mitochondrial membrane, and yeast mutants lacking MPC proteins show severe defects in mitochondrial pyruvate uptake. Coexpression of mouse MPC1 and MPC2 in Lactococcus lactis promoted transport of pyruvate across the membrane (Herzig et al., 2012). Yeast MPC proteins with an odd number of transmembrane segments and a matrix-exposed N-terminus are imported by the carrier pathway, using the receptor Tom70, small TIM chaperones, and the TIM22 complex. The TIM9.10 complex chaperones MPC proteins through the mitochondrial intermembrane space using conserved hydrophobic motifs that are also required for the ...
The Ordinary Buffet - KbeautyCafe PhilippinesThe Ordinary 'Buffet' - KbeautyCafe Philippines
Introducing The Ordinary "Buffet", a multi-peptide serum that targets multiple signs of aging - dryness, fine lines, wrinkles, dullness, and uneven texture. The formula combines a comprehensive array of studied techonologies to combat aging. The technologies are Matrixyl™ 3000 peptide complex (with palmitoyl tetrapeptide-7), Matrixyl™ synthe6™ peptide complex (with palmitoyl tripeptide-38), SYN™-AKE peptide complex (with dipeptide diaminobutyroyl benzylamide diacetate), Relistase peptide complex (with acetylarginyltryptophyl diphenylglycine), ARGIRELOX™ peptide complex (with acetyl hexapeptide-8, pentapeptide-18), Probiotic complex (with lactococcus lactis lysate), in a base of 11 skin-friendly amino acids and multiple hyaluronic acid complexes. The total concentration of these technologies in the formula by weight is a whopping 25.1%!. Start incorporating anti-aging serums as early as in your 20s!. Note from the The Ordinary: It is best to not use Peptides in the same routine as the ...
The Ordinary | Buffet - 30mlThe Ordinary | "Buffet" - 30ml
This formula combines a comprehensive array of studied technologies to target multiple signs of ageing at once. The technologies are Matrixyl 3,000 peptide complex (with palmitoyl tetrapeptide-7), Matrixyl Synthe6 peptide complex (with palmitoyl tripeptide-38), Syn-Ake peptide complex (with dipeptide diaminobutyroyl benzylamide diacetate), Relistase peptide complex (with acetylarginyltryptophyl diphenylglycine), Argirelox peptide complex (with acetyl hexapeptide-8, pentapeptide-18), Probiotic complex (with lactococcus lactis lysate), in a base of 11 skin-friendly amino acids and multiple hyaluronic acid complexes. The total concentration of these technologies in the formula by weight is 25.1%. (References are made to third-party trademarks in this description. All such trademarks are owned by their respective owners. Neither The Ordinary nor DECIEM is affiliated with these trademarks or their owners.) Note: The name of this formula was inspired by some brands referring to
Search ResultsSearch Results
Numerous significant hits to DNA polymerase I sequences; e.g. residues 3-284 are 38% similar to (X98575) DNA-dependent DNA polymerase of Anaerocellum thermophilum; residues 3-286 are 34% similar to (U78771) DNA polymerase I (PolI) of Lactococcus lactis; and residues 2-285 are 32% similar to DP01_STRPN ...
Viral Movie Provides Clues to Infection Mechanism - Scientific AmericanViral Movie Provides Clues to Infection Mechanism - Scientific American
Latest news and features on science issues that matter including earth, environment, and space. Get your science news from the most trusted source!
Pre GI: GenePre GI: Gene
General Information: Bifidobacterium animalis subsp. lactis (strain DSM 10140 / JCM 10602 / LMG 18314) is an anaerobic Gram-positive lactic acid bacterium commonly found in the guts of healthy humans and has been identified in the infant gut biota, particularly in ileal, fecal, and mucosal samples. Some strains of B. animalis subsp. lactis are able to survive in the GIT, to adhere to human epithelial cells in vitro, to modify fecal flora, to modulate the host immune response, or to prevent microbial gastroenteritis and colitis. ...
Bio-purification of plant proteins - NIZO - consortia - Herwig BachmannBio-purification of plant proteins - NIZO - consortia - Herwig Bachmann
The bio-purification of plant protein consortium led by NIZO, will be aimed to develop strategies to remove off-flavours of plant ingredients.
di sini bermula: Diari Jantung Abid: umur sebulan..simptom heart failure mula kelihatandi sini bermula: Diari Jantung Abid: umur sebulan..simptom 'heart failure' mula kelihatan
semasa abid berusia 2 minggu, kami membawanya ke ppukm untuk 1st appointment di klinik paediatrik (kardiologi)..kali ni kami jumpa dengan doktor bilqis (pakar jantung kanak-kanak)..di awal pertemuan doktor memberitahu ohh inilah parent abid ye..doktor bilqis ni sangat tegas orangnya tapi sangat baik..soalan dia kena kita jawab straight tak boleh bunga-bunga konar sana konar sini..kecut perut sokmo kalu nok jawab soalan dia huhu...dari hasil pemeriksaan terhadap abid, doktor bilqis sahkan abid mempunyai 5 masalah pada jantungnya..terduduk seketika sebaik saja doktor bilqis explain kepada kami..sebelum ni masa dalam kandungan kami diberitahu yang abid hanya mengalami masalah vsd sahaja..doktor bilqis terangkan yang dalam kandungan kita tak dapat nak lihat 100% kerana baby dalam kandungan kita tak boleh lihat dengan jelas keseluruhannya..dari perbincangan tu doktor bagitau plan rawatan untuk abid..memang tak da jalan lain kecuali pembedahan dan sejujurnya kami memang dah bersedia mental fizikal ...
Immunomodulatory Properties of Bacterium-Like Particles Obtained From Immunobiotic Lactobacilli: Prospects for Their Use as...Immunomodulatory Properties of Bacterium-Like Particles Obtained From Immunobiotic Lactobacilli: Prospects for Their Use as...
Non-viable lactic acid bacteria (LAB) have been proposed as antigen delivery platforms called bacterium-like particles (BLPs). Most studies have been performed with Lactococcus lactis-derived BLPs where multiple antigens were attached to the peptidoglycan surface and used to successfully induce specific immune responses. It is well-established that the immunomodulatory properties of LAB are strain...
Introduction to Scoliosis Management : PNZIntroduction to Scoliosis Management : PNZ
Addressed to physiotherapists and any health professional interested in acquiring knowledge on scoliosis and other structural alterations of the spine.. Course objectives:. ...
Australian spectrum management principles | ACMAAustralian spectrum management principles | ACMA
A summary of the key influences, organisations and principles that guide the ACMAs approach to spectrum planning. Click here to learn more.
Shell shocked by what BIL has texted me | MumsnetShell shocked by what BIL has texted me | Mumsnet
This is a weird one I know. I am a regular mumsnetter and recently changed my username but this is genuine. I have only changed a couple of details a
PressReader - Bil: 2017-03-18 - Toyo-ta Pri-usPressReader - Bil: 2017-03-18 - Toyo-ta Pri-us
Ef-ter tio mil-jo-ner till-ver-ka-de hy-brid-bi-lar från Toyo-ta lan-se-ras fjär-de ge-ne-ra-tio-nens Pri-us nu som ladd-hy-brid. Och den nya ver-sio-nen lo-var gott.
Jilid42 Bil 11 November 2013Jilid42 Bil 11 November 2013
Shuhaila Ahmad, Norzilawati Mohd Naim, Harlina Harlizah Siraj, Rohana Jaafar, Shareena Ishak, Roslan Harun, Abd Rahman Abd Jamal, Wan Zurinah Wan Ngah & Syed Zulkifli Syed Zakaria ...
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Lactococcus lactisLactococcusNisinCheeseBacterial ProteinsMolecular Sequence DataBacteriophagesBacteriocinsGenes, BacterialSiphoviridaeGene Expression Regulation, BacterialLeuconostocLactobacillusBase SequenceDNA, BacterialFermentationPlasmidsAmino Acid SequenceKluyveromycesCloning, MolecularMilkOperonSequence Analysis, DNALactoseCultured Milk ProductsAcetoinConjugation, GeneticDiacetylOpen Reading FramesStreptococcus thermophilusFood MicrobiologyFood IndustryStreptococcus PhagesGenetic EngineeringCulture MediaPediococcusChromosomes, BacterialDairy ProductsPhosphofructokinasesRestriction MappingEscherichia coliSequence Homology, Amino AcidIndustrial MicrobiologyCholatesBacteriolysisAminopeptidasesLactobacillus plantarumCaseinsDNA Transposable ElementsTransformation, BacterialLactic AcidCell WallFood TechnologyStreptococcusPhosphoglucomutaseBifidobacteriumMicrococcal NucleaseL-Lactate DehydrogenaseLactobacillaceae
Lactococcus lactis - WikipediaLactococcus lactis - Wikipedia
Lactococcus lactis - WikipediaLactococcus lactis - Wikipedia
KEGG PATHWAY: Terpenoid backbone biosynthesis - Lactococcus lactis subsp. cremoris A76KEGG PATHWAY: Terpenoid backbone biosynthesis - Lactococcus lactis subsp. cremoris A76
Lactococcus lactisLactococcus lactis
Draft Genome Sequences of Three Amino Acid-Secreting Lactococcus lactis Strains - Research database - University of GroningenDraft Genome Sequences of Three Amino Acid-Secreting Lactococcus lactis Strains - Research database - University of Groningen
Mucosal Immunization with Recombinant Lactococcus lactis | Springer for Research & DevelopmentMucosal Immunization with Recombinant Lactococcus lactis | Springer for Research & Development
A Genome-Scale Integration and Analysis of Lactococcus lactis Translation Data - InfoscienceA Genome-Scale Integration and Analysis of Lactococcus lactis Translation Data - Infoscience
The First Clinical Use of a Recombinant Lactococcus lactis Expressing Human Papillomavirus Type 16 E7 Oncogene Oral Vaccine: A...The First Clinical Use of a Recombinant Lactococcus lactis Expressing Human Papillomavirus Type 16 E7 Oncogene Oral Vaccine: A...
6-Phosphogluconate dehydrogenase from Lactococcus lactis: a role for arginine residues in binding substrate and coenzyme |...6-Phosphogluconate dehydrogenase from Lactococcus lactis: a role for arginine residues in binding substrate and coenzyme |...
Modeling of nisin production by Lactococcus lactis</em>...Modeling of nisin production by Lactococcus lactis</em>...
Further elucidation of galactose utilization in Lactococcus lactis MG1363Further elucidation of galactose utilization in Lactococcus lactis MG1363
Lactococcus Lactis Probiotics - Lyophilized Probiotics SupplementsLactococcus Lactis Probiotics - Lyophilized Probiotics Supplements
Cloning and expression of a novel lactococcal aggregation factor from Lactococcus lactis subsp. lactis BGKP1 | BMC Microbiology...Cloning and expression of a novel lactococcal aggregation factor from Lactococcus lactis subsp. lactis BGKP1 | BMC Microbiology...
Recombinant porcine epidermal growth factor-secreting Lactococcus lactis promotes the growth performance of early-weaned...Recombinant porcine epidermal growth factor-secreting Lactococcus lactis promotes the growth performance of early-weaned...
Lactococcus lactis peptidoglycaneLactococcus lactis peptidoglycane
Lactococcus lactis subsp. cremoris IBB477Lactococcus lactis subsp. cremoris IBB477
Heterologous gene expression of bovine plasmin in Lactococcus lactis. - PubMed - NCBIHeterologous gene expression of bovine plasmin in Lactococcus lactis. - PubMed - NCBI
RCSB PDB - 5YHZ: Structure of Lactococcus lactis ZitR, E41A mutantRCSB PDB - 5YHZ: Structure of Lactococcus lactis ZitR, E41A mutant
Steady state growth space study of Lactococcus lactis in D-stat cultures | SpringerLinkSteady state growth space study of Lactococcus lactis in D-stat cultures | SpringerLink
Lactococcus lactis subsp. lactis (Lister) Schleifer et al. ATCC ®Lactococcus lactis subsp. lactis (Lister) Schleifer et al. ATCC ®
Protein balance of Lactococcus lactis in cheese production - WURProtein balance of Lactococcus lactis in cheese production - WUR
hom - Homoserine dehydrogenase - Lactococcus lactis subsp. cremoris - hom gene & proteinhom - Homoserine dehydrogenase - Lactococcus lactis subsp. cremoris - hom gene & protein
Standardized assay medium to measure Lactococcus lactis enzyme activities while mimicking intracellular conditions. - PubMed -...Standardized assay medium to measure Lactococcus lactis enzyme activities while mimicking intracellular conditions. - PubMed -...
Frontiers | Further Elucidation of Galactose Utilization in Lactococcus lactis MG1363 | MicrobiologyFrontiers | Further Elucidation of Galactose Utilization in Lactococcus lactis MG1363 | Microbiology
Treatment of Murine Colitis by Lactococcus lactis Secreting Interleukin-10 | ScienceTreatment of Murine Colitis by Lactococcus lactis Secreting Interleukin-10 | Science
KEGG PATHWAY: Biotin metabolism - Lactococcus lactis subsp. lactis Il1403KEGG PATHWAY: Biotin metabolism - Lactococcus lactis subsp. lactis Il1403
CiteSeerX - Controlled gene expression systems for Lactococcus lactis with the food-grade inducer nisinCiteSeerX - Controlled gene expression systems for Lactococcus lactis with the food-grade inducer nisin
Lactococcus lactis | Harvard Catalyst Profiles | Harvard CatalystLactococcus lactis | Harvard Catalyst Profiles | Harvard Catalyst
Frontiers | Cell Wall Anchoring of the Campylobacter Antigens to Lactococcus lactis | MicrobiologyFrontiers | Cell Wall Anchoring of the Campylobacter Antigens to Lactococcus lactis | Microbiology
Lactococcus lactis bacterium - Stock Image - B236/0055 - Science Photo LibraryLactococcus lactis bacterium - Stock Image - B236/0055 - Science Photo Library
Portal vein thrombosis and liver abscess due to Lactococcus lactis.Portal vein thrombosis and liver abscess due to Lactococcus lactis.
Plasmid Transfer via Transduction from Streptococcus thermophilus to Lactococcus lactis | Journal of BacteriologyPlasmid Transfer via Transduction from Streptococcus thermophilus to Lactococcus lactis | Journal of Bacteriology
Inhibitory effect of <i>Lactococcus lactis</i> on the bioactivity of...Inhibitory effect of <i>Lactococcus lactis</i> on the bioactivity of...
Acoustic Emission Signal of Lactococcus lactis before and after Inhibition with NaN3 and Infection with Bacteriophage c2Acoustic Emission Signal of Lactococcus lactis before and after Inhibition with NaN3 and Infection with Bacteriophage c2
A family of genes encoding cold-shock proteins in Lactococcus lactis MG 1363A family of genes encoding cold-shock proteins in Lactococcus lactis MG 1363
Influence of pH on growth and bacteriocin production by Lactococcus lactis subsp. lactis 14ONWC during batch fermentation |...Influence of pH on growth and bacteriocin production by Lactococcus lactis subsp. lactis 14ONWC during batch fermentation |...
Oral Vaccination with the Porcine Rotavirus VP4 Outer Capsid Protein Expressed by Lactococcus lactis Induces Specific Antibody...Oral Vaccination with the Porcine Rotavirus VP4 Outer Capsid Protein Expressed by Lactococcus lactis Induces Specific Antibody...
Use of Bacteriocin Producing Lactococcus lactis subsp. lactis LABW4 to Prevent Listeria monocytogenes Induced Spoilage of MeatUse of Bacteriocin Producing Lactococcus lactis subsp. lactis LABW4 to Prevent Listeria monocytogenes Induced Spoilage of Meat
Oxidative stress in Lactococcus lactis caused by endogenously produced hydrogen peroxide is relieved by asparagineOxidative stress in Lactococcus lactis caused by endogenously produced hydrogen peroxide is relieved by asparagine
AnatomyOrganismsChemicals and DrugsAnalytical, Diagnostic and Therapeutic Techniques and EquipmentPhenomena and ProcessesDisciplines and OccupationsTechnology, Industry, AgricultureInformation ScienceHealth Care
Lactococcus lactisLactococcusNisinCheeseBacterial ProteinsMolecular Sequence DataBacteriophagesBacteriocinsGenes, BacterialSiphoviridaeGene Expression Regulation, BacterialLeuconostocLactobacillusBase SequenceDNA, BacterialFermentationPlasmidsAmino Acid SequenceKluyveromycesCloning, MolecularMilkOperonSequence Analysis, DNALactoseCultured Milk ProductsAcetoinConjugation, GeneticDiacetylOpen Reading FramesStreptococcus thermophilusFood MicrobiologyFood IndustryStreptococcus PhagesGenetic EngineeringCulture MediaPediococcusChromosomes, BacterialDairy ProductsPhosphofructokinasesRestriction MappingEscherichia coliSequence Homology, Amino AcidIndustrial MicrobiologyCholatesBacteriolysisAminopeptidasesLactobacillus plantarumCaseinsDNA Transposable ElementsTransformation, BacterialLactic AcidCell WallFood TechnologyStreptococcusPhosphoglucomutaseBifidobacteriumMicrococcal NucleaseL-Lactate DehydrogenaseLactobacillaceae
StrainsMG1363BacteriumStrainRecombinant LactococcusPlasmidBacteriaFermentationGenesProteinMucosalSecretionGeneticButtermilkMetabolismXyloseGram positiveIntestinalLactic acidAggregationDairy2000LacticStrainsOrganismIL1403Bacterium Lactococcus lactisRecombinant Lactococcus lactisProduced by Lactococcus lactis subspBacteriocinsPathwayProbioticExtensivelyEnhancing Lactococcus lactis subspGene expressionPlasmidsBacteriocin producAnalysis of LactococcusIsolatesBacterialVaccineAntigensGenusIndustriallyAntimicrobialLactobacillusOxidative stressExpression SystemLactococcalGarvieaeCharacterizationNZ3900GlycolyticEscherichiaTranscriptionalGenomeProductionDairy productsListerInhibitionGlycolysisIsolateHeterologous proteinMembrane
Strains7
MG13632
  • in the genome of L. lactis MG1363. (wikipedia.org)
  • A novel plant-niche-related PTS component Llmg_0963 forming a hybrid transporter Llmg_0963PtcBA and a glucose/mannose-specific PTS are shown to be involved in galactose transport in L. lactis MG1363. (ucc.ie)
Bacterium3
  • Lactococcus lactis is a Gram-positive bacterium used extensively in the production of buttermilk and cheese, but has also become famous as the first genetically modified organism to be used alive for the treatment of human disease. (wikipedia.org)
  • lactis is added to milk, the bacterium uses enzymes to produce energy molecules (ATP), from lactose. (wikipedia.org)
  • A bill to designate Lactococcus lactis , a bacterium used in making cheese, as the state microbe has passed the Wisconsin Assembly and is now going to be voted by the state senate. (wordpress.com)
Strain2
Recombinant Lactococcus3
  • Oral and Intra nasal vaccination with recombinant Lactococcus lactis can induce high level immune responses to tetanus toxin fragment C elicits. (springer.com)
  • A recombinant Lactococcus lactis which produced and secreted pEGF at 1000 ng/ml in culture supernatant was generated. (biomedcentral.com)
  • We have generated a recombinant Lactococcus lactis which produced and secreted fully biologically active porcine EGF. (biomedcentral.com)
Plasmid2
Bacteria6
  • L. lactis is one of the best characterized low GC Gram positive bacteria with detailed knowledge on genetics, metabolism and biodiversity. (wikipedia.org)
  • Bacteriophages specific to L. lactis cause significant economic losses each year by preventing the bacteria from fully metabolizing the milk substrate. (wikipedia.org)
  • In Shuichi Nakamura's, Yusuke V. Marimoto, and Seishi Kudo's study, they seek to prove that some fermentation produced by L.lactis can hinder motility in pathogenic bacteria. (wikipedia.org)
  • It is produced by lactic acid bacteria Lactococcus lactis subsp. (elsevier.com)
  • Applications are invited for the position of Junior Research Fellow on SERB sponsored project entitled "Evaluation of immunogenicity and protective efficacy of bivalent vaccine candidate against Shigella and Salmonella using food grade bacteria Lactococcus lactis " under the supervision of Dr. Priti Desai, Institute of Advanced Research, Koba Institutional Area, Koba, Gandhinagar-382 007. (helpbiotech.co.in)
  • L. lactis makes sense for Wisconsin - the bacteria helps make cheese, and Wisconsin is known as the "Cheese State. (wordpress.com)
Fermentation6
Genes2
  • Consistent with the results from comparative genomics (see references above) this resulted in L. lactis losing or down-regulating genes which are dispensable in milk and the up-regulation of peptide transport. (wikipedia.org)
  • We established a procedure for robust estimation of the ribosomal density in hundreds of genes from Lactococcus lactis polysome size measurements. (epfl.ch)
Protein3
Mucosal1
Secretion1
  • The success of this strain's construction helped to inhibit migration and expansion of cancer cells, showing that the secretion properties of L.lactis of this particular peptide illustrates a hopeful future in Cancer therapy. (wikipedia.org)
Genetic1
  • Genetic marking of Lactococcus lactis shows its survival in the human gastrointestinal tract. (springer.com)
Buttermilk1
Metabolism1
Xylose1
  • Lactococcus lactis IO-1 (JCM7638) produces L-lactic acid predominantly when grown at high xylose concentrations, and its utilization is highly desired in the green plastics industry. (elsevier.com)
Gram positive1
Intestinal1
Lactic acid1
  • The capability to produce lactic acid is one of the reasons why L. lactis is one of the most important microorganisms in the dairy industry. (wikipedia.org)
Aggregation4
Dairy2
20001
  • van der Heide, T., and Poolman, B. (2000) Osmoregulated ABC-transport system of Lactococcus lactis senses water stress via changes in the physical state of the membrane. (sciencemag.org)
Lactic22
Strains22
Organism8
IL14034
Bacterium Lactococcus lactis4
Recombinant Lactococcus lactis4
Produced by Lactococcus lactis subsp2
Bacteriocins1
Pathway4
  • New insights into the complex regulation of the glycolytic pathway in Lactococcus lactis. (harvard.edu)
  • The engineering of glycerol metabolism in L. lactis was initiated from three different perspectives: overexpression of glycerol kinase from L. lactis, introduction of a heterologous glycerol assimilation pathway and construction of a library of NADH oxidase activity. (dtu.dk)
  • Ganesan, B, Dobrowolski, P & Weimer, BC 2006, ' Identification of the leucine-to-2-methylbutyric acid catabolic pathway of Lactococcus lactis ', Applied and Environmental Microbiology , vol. 72, no. 6, pp. 4264-4273. (elsevier.com)
  • SubC signal for extracellular localization in B. licheniformis, was also recognized by L. lactis Sec pathway, although with lower efficiency, as shown by a 3-fold lower protease activity in the medium. (duhnnae.com)
Probiotic3
Extensively3
Enhancing Lactococcus lactis subsp1
Gene expression8
Plasmids5
Bacteriocin produc2
Analysis of Lactococcus1
  • Comparative Genome Analysis of Lactococcus lactis Indicates Niche Adaptation and Resolves Genotype/Phenotype Disparity. (uni-bielefeld.de)
Isolates4
Bacterial2
  • lactis may be a useful tool for preventing infections by multiple bacterial species. (balanceone.com)
  • During industrial processes, L. lactis is often exposed to multiple environmental stresses (low and high temperature, low pH, high osmotic pressure, nutrient starvation and oxidation) that can cause loss or reduction of bacterial viability, reproducibility, as well as organoleptic and/or fermentative qualities. (bvsalud.org)
Vaccine5
Antigens4
Genus1
Industrially2
Antimicrobial4
Lactobacillus1
Oxidative stress2
  • The most cost-effective method for the preservation of L. lactis starter cultures is spray drying, but during this process cultures encounter heat and oxidative stress, typically resulting in low survival rates. (uva.nl)
  • During the last two decades, considerable efforts have been made to improve our knowledge of oxidative stress in L. lactis. (bvsalud.org)
Expression System4
Lactococcal2
Garvieae3
Characterization3
NZ39001
Glycolytic2
Escherichia1
  • Expression of SARS-coronavirus nucleocapsid protein in Escherichia coli and Lactococcus lactis for serodiagnosis and mucosal vaccination. (ebscohost.com)
Transcriptional2
Genome4
Production10
Dairy products1
Lister1
Inhibition4
Glycolysis1
Isolate1
Heterologous protein1
Membrane3
Draft Genome Sequences of Three Amino Acid-Secreting Lactococcus lactis Strains - Research database - University of Groningen
Draft Genome Sequences of Three Amino Acid-Secreting Lactococcus lactis Strains - Research database - University of Groningen (rug.nl)
Mucosal Immunization with Recombinant Lactococcus lactis | Springer for Research & Development
Mucosal Immunization with Recombinant Lactococcus lactis | Springer for Research & Development (rd.springer.com)
SWISS-MODEL Repository | Q030Z9
SWISS-MODEL Repository | Q030Z9 (swissmodel.expasy.org)
RCSB PDB - 5YHZ: Structure of Lactococcus lactis ZitR, E41A mutant
RCSB PDB - 5YHZ: Structure of Lactococcus lactis ZitR, E41A mutant (rcsb.org)
Lactococcus lactis bacterium - Stock Image - B236/0055 - Science Photo Library
Lactococcus lactis bacterium - Stock Image - B236/0055 - Science Photo Library (sciencephoto.com)
Steady state growth space study of Lactococcus lactis in D-stat cultures | SpringerLink
Steady state growth space study of Lactococcus lactis in D-stat cultures | SpringerLink (link.springer.com)
CiteSeerX - Citation Query A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the...
CiteSeerX - Citation Query A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the... (citeseer.ist.psu.edu)
Frontiers | Further Elucidation of Galactose Utilization in Lactococcus lactis MG1363 | Microbiology
Frontiers | Further Elucidation of Galactose Utilization in Lactococcus lactis MG1363 | Microbiology (frontiersin.org)
A metagenomic study of the rumen virome in domestic caprids | SpringerLink
A metagenomic study of the rumen virome in domestic caprids | SpringerLink (link.springer.com)
Research news and articles - DTU
Research news and articles - DTU (dtu.dk)
Treatment of Murine Colitis by Lactococcus lactis Secreting Interleukin-10 | Science
Treatment of Murine Colitis by Lactococcus lactis Secreting Interleukin-10 | Science (science.sciencemag.org)
Plasmid Transfer via Transduction from Streptococcus thermophilus to Lactococcus lactis | Journal of Bacteriology
Plasmid Transfer via Transduction from Streptococcus thermophilus to Lactococcus lactis | Journal of Bacteriology (jb.asm.org)
Introduction of Peptidase Genes from Lactobacillus delbrueckii subsp. lactis into Lactococcus lactis and Controlled Expression ...
Introduction of Peptidase Genes from Lactobacillus delbrueckii subsp. lactis into Lactococcus lactis and Controlled Expression ... (aem.asm.org)
Articles of Significant Interest in This Issue | Applied and Environmental Microbiology
Articles of Significant Interest in This Issue | Applied and Environmental Microbiology (aem.asm.org)
Bridge PB93 Strain Passport - StrainInfo
Bridge PB93 Strain Passport - StrainInfo (straininfo.net)
Volume 7, Number 5-October 2001 - Emerging Infectious Diseases journal - CDC
Volume 7, Number 5-October 2001 - Emerging Infectious Diseases journal - CDC (wwwnc.cdc.gov)
Ripening to convert ferment milk sugars to lactic acid : Butter
Ripening to convert ferment milk sugars to lactic acid : Butter (webexhibits.org)
Spain Articles All Issues - Emerging Infectious Diseases journal - CDC
Spain Articles All Issues - Emerging Infectious Diseases journal - CDC (wwwnc.cdc.gov)
Free D -amino acids produced by commensal bacteria in the colonic lumen | Scientific Reports
Free D -amino acids produced by commensal bacteria in the colonic lumen | Scientific Reports (nature.com)
Redwood Hill Farm & Creamery K - Online Groceries | Safeway
Redwood Hill Farm & Creamery K - Online Groceries | Safeway (safeway.com)
Sequence and analysis of the 60 kb conjugative, bacteriocin...: Ingenta Connect
Sequence and analysis of the 60 kb conjugative, bacteriocin...: Ingenta Connect (ingentaconnect.com)
Identification of the Minimal Replicon of Lactococcus lactis subsp. lactis UC317 Plasmid pCI305 | Applied and Environmental...
Identification of the Minimal Replicon of Lactococcus lactis subsp. lactis UC317 Plasmid pCI305 | Applied and Environmental... (aem.asm.org)
Electrophoretic Determination of Lactococcus lactis Modified by Zinc Ions | Springer for Research & Development
Electrophoretic Determination of Lactococcus lactis Modified by Zinc Ions | Springer for Research & Development (rd.springer.com)
Renew Life, FloraSmart, Advanced Probiotic, 12 Billion, 30 Veggie Caplets - iHerb.com
Renew Life, FloraSmart, Advanced Probiotic, 12 Billion, 30 Veggie Caplets - iHerb.com (iherb.com)
Spr1695 - dITP/XTP pyrophosphatase - Streptococcus pneumoniae (strain ATCC BAA-255 / R6) - spr1695 gene & protein
Spr1695 - dITP/XTP pyrophosphatase - Streptococcus pneumoniae (strain ATCC BAA-255 / R6) - spr1695 gene & protein (uniprot.org)
Frontiers | Use of Wild Type or Recombinant Lactic Acid Bacteria as an Alternative Treatment for Gastrointestinal Inflammatory...
Frontiers | Use of Wild Type or Recombinant Lactic Acid Bacteria as an Alternative Treatment for Gastrointestinal Inflammatory... (frontiersin.org)