Specialized phagocytic cells of the MONONUCLEAR PHAGOCYTE SYSTEM found on the luminal surface of the hepatic sinusoids. They filter bacteria and small foreign proteins out of the blood, and dispose of worn out red blood cells.
A large lobed glandular organ in the abdomen of vertebrates that is responsible for detoxification, metabolism, synthesis and storage of various substances.
Gadolinium. An element of the rare earth family of metals. It has the atomic symbol Gd, atomic number 64, and atomic weight 157.25. Its oxide is used in the control rods of some nuclear reactors.
A diphosphonate which affects calcium metabolism. It inhibits bone resorption and soft tissue calcification.
The main structural component of the LIVER. They are specialized EPITHELIAL CELLS that are organized into interconnected plates called lobules.
A layer of epithelium that lines the heart, blood vessels (ENDOTHELIUM, VASCULAR), lymph vessels (ENDOTHELIUM, LYMPHATIC), and the serous cavities of the body.
Toxins closely associated with the living cytoplasm or cell wall of certain microorganisms, which do not readily diffuse into the culture medium, but are released upon lysis of the cells.
Lipid-containing polysaccharides which are endotoxins and important group-specific antigens. They are often derived from the cell wall of gram-negative bacteria and induce immunoglobulin secretion. The lipopolysaccharide molecule consists of three parts: LIPID A, core polysaccharide, and O-specific chains (O ANTIGENS). When derived from Escherichia coli, lipopolysaccharides serve as polyclonal B-cell mitogens commonly used in laboratory immunology. (From Dorland, 28th ed)
Serum glycoprotein produced by activated MACROPHAGES and other mammalian MONONUCLEAR LEUKOCYTES. It has necrotizing activity against tumor cell lines and increases ability to reject tumor transplants. Also known as TNF-alpha, it is only 30% homologous to TNF-beta (LYMPHOTOXIN), but they share TNF RECEPTORS.
A group of inosine ribonucleotides in which the phosphate residues of each inosine ribonucleotide act as bridges in forming diester linkages between the ribose moieties.
The engulfing and degradation of microorganisms; other cells that are dead, dying, or pathogenic; and foreign particles by phagocytic cells (PHAGOCYTES).
INFLAMMATION of the LIVER.
Liver diseases associated with ALCOHOLISM. It usually refers to the coexistence of two or more subentities, i.e., ALCOHOLIC FATTY LIVER; ALCOHOLIC HEPATITIS; and ALCOHOLIC CIRRHOSIS.
Genetically identical individuals developed from brother and sister matings which have been carried out for twenty or more generations or by parent x offspring matings carried out with certain restrictions. This also includes animals with a long history of closed colony breeding.
A spectrum of clinical liver diseases ranging from mild biochemical abnormalities to ACUTE LIVER FAILURE, caused by drugs, drug metabolites, and chemicals from the environment.
Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others.
Pathological processes of the LIVER.
A strain of albino rat used widely for experimental purposes because of its calmness and ease of handling. It was developed by the Sprague-Dawley Animal Company.
A strain of albino rat developed at the Wistar Institute that has spread widely at other institutions. This has markedly diluted the original strain.
Mononuclear cells with pronounced phagocytic ability that are distributed extensively in lymphoid and other organs. It includes MACROPHAGES and their precursors; PHAGOCYTES; KUPFFER CELLS; HISTIOCYTES; DENDRITIC CELLS; LANGERHANS CELLS; and MICROGLIA. The term mononuclear phagocyte system has replaced the former reticuloendothelial system, which also included less active phagocytic cells such as fibroblasts and endothelial cells. (From Illustrated Dictionary of Immunology, 2d ed.)
The relatively long-lived phagocytic cell of mammalian tissues that are derived from blood MONOCYTES. Main types are PERITONEAL MACROPHAGES; ALVEOLAR MACROPHAGES; HISTIOCYTES; KUPFFER CELLS of the liver; and OSTEOCLASTS. They may further differentiate within chronic inflammatory lesions to EPITHELIOID CELLS or may fuse to form FOREIGN BODY GIANT CELLS or LANGHANS GIANT CELLS. (from The Dictionary of Cell Biology, Lackie and Dow, 3rd ed.)
A class of nongenotoxic CARCINOGENS that induce the production of hepatic PEROXISOMES and induce hepatic neoplasms after long-term administration.
Glycolipid-anchored membrane glycoproteins expressed on cells of the myelomonocyte lineage including monocytes, macrophages, and some granulocytes. They function as receptors for the complex of lipopolysaccharide (LPS) and LPS-binding protein.
A milky, product excreted from the latex canals of a variety of plant species that contain cauotchouc. Latex is composed of 25-35% caoutchouc, 60-75% water, 2% protein, 2% resin, 1.5% sugar & 1% ash. RUBBER is made by the removal of water from latex.(From Concise Encyclopedia Biochemistry and Molecular Biology, 3rd ed). Hevein proteins are responsible for LATEX HYPERSENSITIVITY. Latexes are used as inert vehicles to carry antibodies or antigens in LATEX FIXATION TESTS.
An enzyme that catalyzes the conversion of L-alanine and 2-oxoglutarate to pyruvate and L-glutamate. (From Enzyme Nomenclature, 1992) EC 2.6.1.2.
A large group of structurally diverse cell surface receptors that mediate endocytic uptake of modified LIPOPROTEINS. Scavenger receptors are expressed by MYELOID CELLS and some ENDOTHELIAL CELLS, and were originally characterized based on their ability to bind acetylated LOW-DENSITY LIPOPROTEINS. They can also bind a variety of other polyanionic ligand. Certain scavenger receptors can internalize micro-organisms as well as apoptotic cells.
Experimentally induced chronic injuries to the parenchymal cells in the liver to achieve a model for LIVER CIRRHOSIS.
Adverse functional, metabolic, or structural changes in ischemic tissues resulting from the restoration of blood flow to the tissue (REPERFUSION), including swelling; HEMORRHAGE; NECROSIS; and damage from FREE RADICALS. The most common instance is MYOCARDIAL REPERFUSION INJURY.
Cell surface proteins that bind lipoproteins with high affinity. Lipoprotein receptors in the liver and peripheral tissues mediate the regulation of plasma and cellular cholesterol metabolism and concentration. The receptors generally recognize the apolipoproteins of the lipoprotein complex, and binding is often a trigger for endocytosis.
Cells that can carry out the process of PHAGOCYTOSIS.
The process of altering the morphology and functional activity of macrophages so that they become avidly phagocytic. It is initiated by lymphokines, such as the macrophage activation factor (MAF) and the macrophage migration-inhibitory factor (MMIF), immune complexes, C3b, and various peptides, polysaccharides, and immunologic adjuvants.
Mononuclear phagocytes derived from bone marrow precursors but resident in the peritoneum.
Nitric acid (HNO3). A colorless liquid that is used in the manufacture of inorganic and organic nitrates and nitro compounds for fertilizers, dye intermediates, explosives, and many different organic chemicals. Continued exposure to vapor may cause chronic bronchitis; chemical pneumonitis may occur. (From Merck Index, 11th ed)
Nanometer-sized particles that are nanoscale in three dimensions. They include nanocrystaline materials; NANOCAPSULES; METAL NANOPARTICLES; DENDRIMERS, and QUANTUM DOTS. The uses of nanoparticles include DRUG DELIVERY SYSTEMS and cancer targeting and imaging.
They are oval or bean shaped bodies (1 - 30 mm in diameter) located along the lymphatic system.
Instruments used for injecting or withdrawing fluids. (Stedman, 25th ed)
The tunnel in the lower anterior ABDOMINAL WALL through which the SPERMATIC CORD, in the male; ROUND LIGAMENT, in the female; nerves; and vessels pass. Its internal end is at the deep inguinal ring and its external end is at the superficial inguinal ring.
A worm-like blind tube extension from the CECUM.
A bibliographic database that includes MEDLINE as its primary subset. It is produced by the National Center for Biotechnology Information (NCBI), part of the NATIONAL LIBRARY OF MEDICINE. PubMed, which is searchable through NLM's Web site, also includes access to additional citations to selected life sciences journals not in MEDLINE, and links to other resources such as the full-text of articles at participating publishers' Web sites, NCBI's molecular biology databases, and PubMed Central.
A publication issued at stated, more or less regular, intervals.
"The business or profession of the commercial production and issuance of literature" (Webster's 3d). It includes the publisher, publication processes, editing and editors. Production may be by conventional printing methods or by electronic publishing.
The premier bibliographic database of the NATIONAL LIBRARY OF MEDICINE. MEDLINE® (MEDLARS Online) is the primary subset of PUBMED and can be searched on NLM's Web site in PubMed or the NLM Gateway. MEDLINE references are indexed with MEDICAL SUBJECT HEADINGS (MeSH).
Repair or renewal of hepatic tissue.
The physiological renewal, repair, or replacement of tissue.
Interferon secreted by leukocytes, fibroblasts, or lymphoblasts in response to viruses or interferon inducers other than mitogens, antigens, or allo-antigens. They include alpha- and beta-interferons (INTERFERON-ALPHA and INTERFERON-BETA).
Analgesic antipyretic derivative of acetanilide. It has weak anti-inflammatory properties and is used as a common analgesic, but may cause liver, blood cell, and kidney damage.
Lists of words, usually in alphabetical order, giving information about form, pronunciation, etymology, grammar, and meaning.
Works containing information articles on subjects in every field of knowledge, usually arranged in alphabetical order, or a similar work limited to a special field or subject. (From The ALA Glossary of Library and Information Science, 1983)

Pronase destroys the lipopolysaccharide receptor CD14 on Kupffer cells. (1/1142)

CD14 is a lipopolysaccharide (LPS) receptor distributed largely in macrophages, monocytes, and neutrophils; however, the role of CD14 in activation of Kupffer cells by LPS remains controversial. The purpose of this study was to determine if different methods used to isolate Kupffer cells affect CD14. Kupffer cells were isolated by collagenase (0.025%) or collagenase-Pronase (0.02%) perfusion and differential centrifugation using Percoll gradients and cultured for 24 h before experiments. CD14 mRNA was detected by RT-PCR from Kupffer cell total RNA as well as from peritoneal macrophages. Western blotting showed that Kupffer cells prepared with collagenase possess CD14; however, it was absent in cells obtained by collagenase-Pronase perfusion. Intracellular calcium in Kupffer cells prepared with collagenase was increased transiently to levels around 300 nM by addition of LPS with 5% rat serum, which contains LPS binding protein. This increase in intracellular calcium was totally serum dependent. Moreover, LPS-induced increases in intracellular calcium in Kupffer cells were blunted significantly (40% of controls) when cells were treated with phosphatidylinositol-specific phospholipase C, which cleaves CD14 from the plasma membrane. However, intracellular calcium did not increase when LPS was added to cells prepared by collagenase-Pronase perfusion even in the presence of serum. These cells were viable, however, because ATP increased intracellular calcium to the same levels as cells prepared with collagenase perfusion. Tumor necrosis factor-alpha (TNF-alpha) mRNA was increased in Kupffer cells prepared with collagenase perfusion 1 h after addition of LPS, an effect potentiated over twofold by serum; however, serum did not increase TNF-alpha mRNA in cells isolated via collagenase-Pronase perfusion. Moreover, treatment with Pronase rapidly decreased CD14 on mouse macrophages (RAW 264.7 cells) and Kupffer cells. These findings indicate that Pronase cleaves CD14 from Kupffer cells, whereas collagenase perfusion does not, providing an explanation for why Kupffer cells do not exhibit a CD14-mediated pathway when prepared with procedures using Pronase. It is concluded that Kupffer cells indeed contain a functional CD14 LPS receptor when prepared gently.  (+info)

Effects of Ro 31-8220 on lipopolysaccharides-induced hepatotoxicity and release of tumor necrosis factor from rat Kupffer cells. (2/1142)

AIM: To investigate protein kinase C (PKC) functions on lipopolysaccharide (LPS)-induced hepatotoxicity, a new potent PKC inhibitor Ro 31-8220 (Ro) was used to detect its effect on LPS-induced hepatotoxicity in rat hepatocytes and tumor necrosis factor (TNF) release from rat Kupffer cells (KC). METHODS: Hepatocytes (containing KC) were incubated with LPS (10 mg.L-1) and Ro (0.1-10 mumol.L-1) for 24 h, alanine aminotransferase (AlaA) leakage in the culture as indication of hepatotoxicity. The TNF activity in the supernatant of rat KC culture with LPS in the presence of Ro (0.1-10 mumol.L-1) was monitored by the L929 target cell lytic assay. RESULTS: Ro (0.1-10 mumol.L-1) reduced AlaA leakage in the hepatocyte culture. Ro inhibited dose-dependently the LPS-induced TNF production from rat KC. CONCLUSION: PKC inhibitor Ro protects the hepatocytes from LPS-induced cytotoxicity and inhibits the LPS-induced TNF production from rat KC.  (+info)

Influences of Kupffer cell stimulation and suppression on immunological liver injury in mice. (3/1142)

AIM: To study the possible involvement of Kupffer cells (KC) in immunological liver injury in mice. METHODS: Liver injury was induced by i.v. injection of Bacillus Calmette-Guerin (BCG) 5 x 10(7) viable bacilli followed by i.v. injection of lipopolysaccharides (LPS) 7.5 micrograms to each mouse. Indian ink and silica were i.v. injected to suppress KC and retinol was given po to stimulate KC in these mice. Plasma alanine aminotransferase (AlaAT), aspatate aminotransferase (AspAT), nitric oxide (NO), and liver tissue were examined. RESULTS: Injection of LPS following BCG injection resulted in a remarkable elevation of plasma NO, AlaAT, and AspAT levels, and severe liver damage. The damages were enhanced by the activation of KC with retinol and reduced by suppression of KC with silica and Indian ink. CONCLUSION: The degree of liver injury induced by BCG + LPS is closely correlated with the status of KC, and NO from KC plays an important role in the pathogenesis of the liver damage in mice.  (+info)

Febrile-range temperature modifies early systemic tumor necrosis factor alpha expression in mice challenged with bacterial endotoxin. (4/1142)

Fever improves survival in acute infections, but the effects of increased core temperature on host defenses are poorly understood. Tumor necrosis factor alpha (TNF-alpha) is an early activator of host defenses and a major endogenous pyrogen. TNF-alpha expression is essential for survival in bacterial infections but, if disregulated, can cause tissue injury. In this study, we show that passively increasing core temperature in mice from the basal (36.5 to 37.5 degrees C) to the febrile (39.5 to 40 degrees C) range modifies systemic TNF-alpha expression in response to bacterial endotoxin (lipopolysaccharide). The early TNF-alpha secretion rate is enhanced, but the duration of maximal TNF-alpha production is shortened. We identified Kupffer cells as the predominant source of the excess TNF-alpha production in the warmer animals. The enhanced early TNF-alpha production observed at the higher temperature in vivo could not be demonstrated in isolated Kupffer cells or in precision-cut liver slices in vitro, indicating the participation of indirect pathways. Therefore, expression of the endogenous pyrogen TNF-alpha is regulated by increments in core temperature during fever, generating an enhanced early, self-limited TNF-alpha pulse.  (+info)

Intravenous glycine improves survival in rat liver transplantation. (5/1142)

In situ manipulation by touching, retracting, and moving liver lobes gently during harvest dramatically reduces survival after transplantation (P. Schemmer, R. Schoonhoven, J. A. Swenberg, H. Bunzendahl, and R. G. Thurman. Transplantation 65: 1015-1020, 1998). The development of harvest-dependent graft injury upon reperfusion can be prevented with GdCl3, a rare earth metal and Kupffer cell toxicant, but it cannot be used in clinical liver transplantation because of its potential toxicity. Thus the effect of glycine, which prevents activation of Kupffer cells, was assessed here. Minimal dissection of the liver for 12 min plus 13 min without manipulation had no effect on survival (100%). However, gentle manipulation decreased survival to 46% in the control group. Furthermore, serum transaminases and liver necrosis were elevated 4- to 12-fold 8 h after transplantation. After organ harvest, the rate of entry and exit of fluorescein dextran, a dye confined to the vascular space, was decreased about twofold, indicating disturbances in the hepatic microcirculation. Pimonidazole binding, which detects hypoxia, increased about twofold after organ manipulation, and Kupffer cells isolated from manipulated livers produced threefold more tumor necrosis factor-alpha after lipopolysaccharide than controls. Glycine given intravenously to the donor increased the serum glycine concentration about sevenfold and largely prevented the effect of gentle organ manipulation on all parameters studied. These data indicate for the first time that pretreatment of donors with intravenous glycine minimizes reperfusion injury due to organ manipulation during harvest and after liver transplantation.  (+info)

A comparison of the pharmacological properties of carbohydrate remodeled recombinant and placental-derived beta-glucocerebrosidase: implications for clinical efficacy in treatment of Gaucher disease. (6/1142)

The objective of these studies was to characterize the macrophage mannose receptor binding and pharmacological properties of carbohydrate remodeled human placental-derived and recombinant beta-glucocerebrosidase (pGCR and rGCR, respectively). These are similar but not identical molecules that were developed as enzyme replacement therapies for Gaucher disease. Both undergo oligosaccharide remodeling during purification to expose terminal mannose sugar residues. Competitive binding data indicated carbohydrate remodeling improved targeting to mannose receptors over native enzyme by two orders of magnitude. Mannose receptor dissociation constants (Kd) for pGCR and rGCR were each 13 nmol/L. At 37 degrees C, 95% of the total macrophage binding was mannose receptor specific. In vivo, pGCR and rGCR were cleared from circulation by a saturable pathway. The serum half-life (t1/2) was 3 minutes when less than saturable amounts were injected intravenously (IV) into mice. Twenty minutes postdose, beta-glucocerebrosidase activity increased over endogenous levels in all tissues examined. Fifty percent of the injected activity was recovered. Ninety-five percent of recovered activity was in the liver. Parenchymal cells (PC), Kupffer cells (KC), and liver endothelium cells (LEC) were responsible for 75%, 22%, and 3%, respectively, of the hepatocellular uptake of rGCR and for 76%, 11%, and 12%, respectively, of the hepatocellular uptake of pGCR. Both molecules had poor stability in LEC and relatively long terminal half-lives in PC (t1/2 = 2 days) and KC (t1/2 = 3 days).  (+info)

Infection of primary cultures of human Kupffer cells by Dengue virus: no viral progeny synthesis, but cytokine production is evident. (7/1142)

We investigated the ability of dengue virus to invade human primary Kupffer cells and to complete its life cycle. The virus effectively penetrated Kupffer cells, but the infection did not result in any viral progeny. Dengue virus-replicating Kupffer cells underwent apoptosis and were cleared by phagocytosis. Infected Kupffer cells produced soluble mediators that could intervene in dengue virus pathogenesis.  (+info)

Prevention of Kupffer cell-induced oxidant injury in rat liver by atrial natriuretic peptide. (8/1142)

The generation of reactive oxygen species (ROS) by activated Kupffer cells contributes to liver injury following liver preservation, shock, or endotoxemia. Pharmacological interventions to protect liver cells against this inflammatory response of Kupffer cells have not yet been established. Atrial natriuretic peptide (ANP) protects the liver against ischemia-reperfusion injury, suggesting a possible modulation of Kupffer cell-mediated cytotoxicity. Therefore, we investigated the mechanism of cytoprotection by ANP during Kupffer cell activation in perfused rat livers of male Sprague-Dawley rats. Activation of Kupffer cells by zymosan (150 microgram/ml) resulted in considerable cell damage, as assessed by the sinusoidal release of lactate dehydrogenase and purine nucleoside phosphorylase. Cell damage was almost completely prevented by superoxide dismutase (50 U/ml) and catalase (150 U/ml), indicating ROS-related liver injury. ANP (200 nM) reduced Kupffer cell-induced injury via the guanylyl cyclase-coupled A receptor (GCA receptor) and cGMP: mRNA expression of the GCA receptor was found in hepatocytes, endothelial cells, and Kupffer cells, and the cGMP analog 8-bromo-cGMP (8-BrcGMP; 50 microM) was as potent as ANP in protecting from zymosan-induced cell damage. ANP and 8-BrcGMP significantly attenuated the prolonged increase of hepatic vascular resistance when Kupffer cell activation occurred. Furthermore, both compounds reduced oxidative cell damage following infusion of H2O2 (500 microM). In contrast, superoxide anion formation of isolated Kupffer cells was not affected by ANP and only moderately reduced by 8-BrcGMP. In conclusion, ANP protects the liver against Kupffer cell-related oxidant stress. This hormonal protection is mediated via the GCA receptor and cGMP, suggesting that the cGMP receptor plays a critical role in controlling oxidative cell damage. Thus ANP signaling should be considered as a new pharmacological target for protecting liver cells against the inflammatory response of activated Kupffer cells without eliminating the vital host defense function of these cells.  (+info)

TY - JOUR. T1 - The proinflammatory myeloid cell receptor TREM-1 controls Kupffer cell activation and development of hepatocellular carcinoma. AU - Wu, Juan. AU - Li, Jiaqi. AU - Salcedo, Rosalba. AU - Mivechi, Nahid F.. AU - Trinchieri, Giorgio. AU - Horuzsko, Anatolij. N1 - Copyright: Copyright 2013 Elsevier B.V., All rights reserved.. PY - 2012/8/15. Y1 - 2012/8/15. N2 - Chronic inflammation drives liver cancer pathogenesis, invasion, andmetastasis. Liver Kupffer cells have crucial roles in mediating the inflammatory processes that promote liver cancer, but the mechanistic basis for their contributions are not fully understood. Here we show that expression of the proinflammatory myeloid cell surface receptor TREM-1 expressed by Kupffer cells is a crucial factor in the development and progression of liver cancer. Deletion of the murine homolog Trem1 in mice attenuated hepatocellular carcinogenesis triggered by diethylnitrosamine (DEN). Trem1 deficiency attenuated Kupffer cell activation by ...
Inhibition of tumor necrosis factor release from cultured rat Kupffer cells by agents that reduce graft failure from storage injury.
TY - JOUR. T1 - Cd18/ICAM-1-dependent oxidafive NF-κB activation leading to nitric oxide production in rat Kupffer cells cocultured with syngeneic hepatoma cells. AU - Kurose, Iwao. AU - Saito, Hidetsugu. AU - Miura, Soichiro. AU - Ebinuma, Hirotoshi. AU - Higuchi, Hajime. AU - Watanabe, Naoyuki. AU - Zeki, Shigeyuki. AU - Nakamura, Tetsuya. AU - Takaishi, Masaaki. AU - Ishii, Hiromasa. PY - 1997/3/1. Y1 - 1997/3/1. N2 - Previous studies have indicated that nitric oxide (NO) released from Kupffer cells modulates biological viability of cocultured hepatoma cells. This study was designed to evaluate the mechanisms by which Kupffer cells synthesize and release NO in response to cocultured hepatoma cells. Kupffer cells isolated from male Wistar rats were cocultured with rat hepatoma cell line, AH70 cells. The sum of nitrite and nitrate levels increased in the culture medium of Kupffer cells with AH70 cells as compared with those of Kupffer cells or AH70 cells alone. Increased expressions of iNOS ...
Parasitized erythrocytes are ingested by murine hepatic macrophages during malaria infection. We non-invasively monitored how this altered the motion of intracellular phagosomes in Kupffer cells using magnetometry. Submicrometric γFe2O3 particles were injected prior to malaria infection. They were cleared from the blood, primarily by Kupffer cells, and retained within their phagosomes. The mice were periodically magnetized. After removing this external magnet, the aligned iron particles created a remnant magnetic field (RMF) which then decayed (relaxation), reflecting the motion of particle-containing phagosomes. After baseline measurements of relaxation, the mice were injected intravenously with Plasmodium chabaudi-parasitized or normal murine red blood cells (RBCs). During the next 15 days, relaxation measurements, parasitaemia and haematocrit values were monitored. At 6 days post injection with 3 × 107 parasitized RBCs, relaxation rates had decreased. At this time, all mice had ...
Kupffer cells (KC), the resident liver macrophages, constitute the liver sinusoids together with other cells such as sinusoidal endothelial cells, hepatic stellate cells, liver-specific natural killer cells and dendritic cells. KC account for approximately 10-15% of the total liver cell population and represent 80-90% of tissue macrophages in the reticuloendothelial system. KC represent an important component of innate immunity.1 2 One characteristic of innate immunity is the rapid response to potentially dangerous stimuli. This suggests a central role of the liver in systemic and regional immune response, because KC come in contact with all the microbiological debris from the gastrointestinal tract reaching the liver via the portal vein.3. KC express the scavenger receptor CD163; CD163 is involved in the clearance and endocytosis of the haemoglobin-haptoglobin complex.4 Once erythrocytes or the haemoglobin-haptoglobin complex has been taken up by KC, the heme delivered from haemoglobin is ...
As much as 90% of an intravenously (i.v.) injected dose of adenovirus serotype 5 (Ad5) is absorbed and destroyed by liver Kupffer cells. Viruses that escape these cells can then transduce hepatocytes after binding factor X (FX). Given that interactions with FX and Kupffer cells are thought to occur on the Ad5 hexon protein, we replaced its exposed hypervariable regions (HVR) with those from Ad6. When tested in vivo in BALB/c mice and in hamsters, the Ad5/6 chimera mediated |10 times higher transduction in the liver. This effect was not due to changes in FX binding. Rather, Ad5/6 appeared to escape Kupffer cell uptake as evidenced by producing no Kupffer cell death in vivo, not requiring predosing in vivo, and being phagocytosed less efficiently by macrophages in vitro compared to Ad5. When tested as a helper-dependent adenovirus (Ad) vector, Ad5/6 mediated higher luciferase and factor IX transgene expression than either helper-dependent adenoviral 5 (HD-Ad5) or HD-Ad6 vectors. These data suggest that
Primary human Kupffer cells from healthy donors. Uncultured human Kupffer cells together with optimized Kupffer cell Plating & Maintenance Medium.
TY - JOUR. T1 - Iron activates NF-κB in Kupffer cells. AU - She, Hongyun. AU - Xiong, Shigang. AU - Lin, Min. AU - Zandi, Ebrahim. AU - Giulivi, Cecilia R. AU - Tsukamoto, Hidekazu. PY - 2002/9. Y1 - 2002/9. N2 - Iron exacerbates various types of liver injury in which nuclear factor (NF)-κB-driven genes are implicated. This study tested a hypothesis that iron directly elicits the signaling required for activation of NF-κB and stimulation of tumor necrosis factor (TNF)-α gene expression in Kupffer cells. Addition of Fe2- but not Fe3+ (∼-5-50 μM) to cultured rat Kupffer cells increased TNF-α. release and TNF-α. promoter activity in a NF-κB-dependent manner. Cu+ but not Cu2+ stimulated TNF-α protein release and promoter activity but with less potency. Fe2+ caused a disappearance of the cytosolic inhibitor κBα, a concomitant increase in nuclear p65 protein, and increased DNA binding of p50/p50 and p65/p50 without affecting activator protein-1 binding. Addition of Fe2- to the cells ...
The liver is known to favor the induction of immunological tolerance rather than immunity. Although Kupffer cells (KC) have been indicated to play a role in liver tolerance to allografts and soluble antigens, the mechanisms involved remain unclear. We hypothesized that KC could induce T cell tolerance by acting as ¡°poor¡± antigen presenting cells, as well as an active suppressor of T cell activation. The phenotypes of KC were characterized by flow cytometry. The abilities of KC to activate T cells and to suppress T cell activation induced by spleen dendritic cells (DC) were examined by in vitro proliferation assays using OTII cells. We found that, comparing with DC, KC expressed significantly lower levels of MHCII, B7.1, B7.2, and CD40. This is consistent with our observation that KC were not as potent as DC in eliciting OVA-specific T cell proliferation. More importantly, we found that KC could inhibit DC-induced OVA-specific T cell response. Further investigation of the mechanism involved ...
This study identifies the use of an osmolyte strategy in liver macrophages. Betaine serves as an osmolyte in Kupffer cells, whose transport is induced in response to increases of ambient osmolarity. Regulation of this transporter as well as betaine availability may represent a novel regulation site …
Definition of Kupffer cell in the Legal Dictionary - by Free online English dictionary and encyclopedia. What is Kupffer cell? Meaning of Kupffer cell as a legal term. What does Kupffer cell mean in law?
Although recruited macrophages and Kupffer cells exhibit similarities, they can be distinguished by several markers. Recruited macrophages are CD11bhiF4/80lo/int cells. In contrast, resident Kupffer cells are CD11bloF4/80hi (58). Generally, high expression of CX3CR1 and low expression of the myeloid marker Ly6c characterize patrolling monocytes (59). Kupffer cells are characterized by a lack of CX3CR1 expression (60). Using CX3CR1-GFP transgenic mice, a gene expression analysis of recruited macrophages and Kupffer cells (60) revealed that both Kupffer cells and recruited macrophages highly express TGF-β, suggesting that both cells contribute to liver fibrogenesis. Further, the infiltrating macrophages are Ly6chi and differentiate into Ly6clo macrophages in acetaminophen-induced liver injury (60). Similar trans-differentiation is observed in alcoholic liver injury (58). In NAFLD and alcoholic liver injury, inflammation is predominantly mediated by recruited macrophages, and Kupffer cells exhibit ...
The influence of acute and chronic alcohol exposure on innate immune cells from peripheral blood monocytes to tissue macrophages originating from spleen, lungs, and liver can be diverse. Earlier studies have shown that sterilization of the gut with antibiotics abrogated the in vivo effects of acute alcohol on Kupffer cells, indicating that circulating endotoxin played a key role in the effects of alcohol (32). Furthermore, in vivo acute alcohol exposure of murine Kupffer cells show decreased responsiveness to LPS, and this has been related to decreased IRAK expression, reduced NFκB activity (33), and decreased intracellular Ca2+ concentration (32). Subsequent studies also revealed that acute in vivo ethanol exposure of Kupffer cells increased CD14 expression through a mechanism dependent on AP-1 activation (34). In addition, splenic and alveolar macrophages exposed to a single dose of in vivo alcohol followed by ex vivo stimulation of LPS showed a significant decrease in proinflammatory ...
Approximately 200 million people throughout the world are infected with hepatitis C virus (HCV). One of the most striking features of HCV infection is its high propensity to establish persistence (∼70-80%) and progressive liver injury. Galectins are evolutionarily conserved glycan-binding proteins with diverse roles in innate and adaptive immune responses. Here, we demonstrate that galectin-9, the natural ligand for the T cell immunoglobulin domain and mucin domain protein 3 (Tim-3), circulates at very high levels in the serum and its hepatic expression (particularly on Kupffer cells) is significantly increased in patients with chronic HCV as compared to normal controls. Galectin-9 production from monocytes and macrophages is induced by IFN-γ, which has been shown to be elevated in chronic HCV infection. In turn, galectin-9 induces pro-inflammatory cytokines in liver-derived and peripheral mononuclear cells; galectin-9 also induces anti-inflammatory cytokines from peripheral but not hepatic ...
Kupffer cells are resident macrophages of the liver (brown cells below). Increased numbers of Kupffer cells are associated with acute and chronic responses of the liver to toxic compounds. We assess the number of Kupffer cells per unit area as a marker of toxicity to help you evaluate toxicological responses in the liver. ...
Kupffer cells were isolated from H-2Kb-tsA58 transgenic mice, which stably express a thermolabile mutant of the Simian virus 40 (SV40) large tumor antigen under the control of a histocompatibility gene promoter (H-2Kb). Cells isolated from this mouse grow continuously at the permissive temperature of 33 °C, at which the mutant tsA58 is active, but dont grow, or there is less growth under the normal culture temperature of 37 °C. Growth is initiated by the incubation of cells with interferon-γ, which activates histocompatibility genes.. In the present project, we harvested Kupffer cells by collagenase perfusion of the liver, gradient centrifugation and subsequent counterflow centrifugation. Four lines were generated by culture at 33 C,in a medium containing interferon-γ and conditioned media from a hepatocyte and an endothelial cell line. In the absence of these paracrine growth factors, we observed a gradual loss of phenotype and secretory function.. One out of 4 clones (KC13-2) obtained by ...
Clone REA231 recognizes CD72, a 45 kDa type II membrane protein with a C-type lectin-like domain. Expression of CD72 is found on most B-lineage cells, follicular dendritic cells, mast cells, and liver Kupffer cells. CD72 is recognized as negative regulator of B cell receptor (BCR) signaling, as well as regulating mast cell growth and differentiation via KIT activation. The inhibitory role of CD72 is exerted via the ITIM sequence in the cytoplasmic tail.Additional information: Clone REA231 displays negligible binding to Fc receptors. - Canada
Supravital preparations of blood and haemopoietic tissues have been studied by interference contrast, phase contrast and conventional light microscopy to help identify the different categories of the smaller blood cells of lampreys. These methods were also used in conjunction with an examination of sectioned material and fixed blood films, to investigate macrophage activity by the blood cells and the liver Kupffer cells. The diameter of the smallest living blood cells was 4-5 ,mUm. When these produced extensive clotting fibres, particularly in the presence of foreign substances, they were obviously thrombocytes, whereas others, some of which contained prominent nucleoli, were considered to represent small lymphocytes and stem cells. Slightly larger cells (5-6 ,mUm), in which the nuclearcytoplasmic ratio was slightly lower and granules were being formed, represented the first stage in the formation of neutrophilic and eosinophilic granulocytes. The early members of the erythrocyte lineage could ...
TY - JOUR. T1 - Distinct patterns of nitric oxide production in hepatic macrophages and endothelial cells following acute exposure of rats to endotoxin. AU - Laskin, D. L.. AU - Heck, D. E.. AU - Gardner, C. R.. AU - Feder, L. S.. AU - Laskin, J. D.. PY - 1994/12/1. Y1 - 1994/12/1. N2 - Hepatic macrophages and endothelial cells play an important role in the clearance of endotoxin from the portal circulation. These cells are activated by endotoxin to release reactive mediators including superoxide anion, hydrogen peroxide, and nitric oxide, which have been implicated in hepatic inflammation and tissue injury. In the present studies we analyzed mechanisms regulating the production of nitric oxide by hepatic macrophages and endothelial cells following in vivo exposure to endotoxin. Rats were injected intravenously with Escherichia coli lipopolysaccharide (LPS, 5 mg/kg). Cells were isolated from the animals 48 h later by in situ perfusion of the liver with collagenase and pronase followed by ...
Patricio Godoy, Nicola J. Hewitt, Ute Albrecht, Melvin E. Andersen, Nariman Ansari, Sudin Bhattacharya, Johannes Georg Bode, Jennifer Bolleyn, Christoph Borner, Jan Böttger, Albert Braeuning, Robert A. Budinsky, Britta Burkhardt, Neil R. Cameron, Giovanni Camussi, Chong-Su Cho, Yun-Jaie Choi, J. Craig Rowlands, Uta Dahmen, Georg Damm, Olaf Dirsch, María Teresa Donato, Jian Dong, Steven Dooley, Dirk Drasdo, Rowena Eakins, Karine Sá Ferreira, Valentina Fonsato, Joanna Fraczek, Rolf Gebhardt, Andrew Gibson, Matthias Glanemann, Chris E. P. Goldring, María José Gómez-Lechón, Geny M. M. Groothuis, Lena Gustavsson, Christelle Guyot, David Hallifax, Seddik Hammad, Adam Hayward, Dieter Häussinger, Claus Hellerbrand, Philip Hewitt, Stefan Hoehme, Hermann-Georg Holzhütter, J. Brian Houston, Jens Hrach, Kiyomi Ito, Hartmut Jaeschke, Verena Keitel, Jens M. Kelm, B. Kevin Park, Claus Kordes, Gerd A. Kullak-Ublick, Edward L. LeCluyse, Peng Lu, Jennifer Luebke-Wheeler, Anna Lutz, Daniel J. Maltman, ...
Kupffer cells (KC), the resident macrophages of the liver, represent the largest population of mononuclear phagocytes in the body. Phenotypic, developmental, and functional aspects of these cells in...
The hepatic failure associated with severe sepsis is characterized by specific, progressive, and often irreversible defects in hepatocellular metabolism (1). Although the etiologic microbe can often be identified, the direct causes and mechanisms of the hepatocellular dysfunction are poorly understood. We have hypothesized that Kupffer cells (KC), which interact with ambient septic stimuli, respond by providing signals to adjacent hepatocytes (HC) in sepsis . Furthermore, we have provided evidence (2, 3) that KC activated by LPS from Gram-negative bacteria can induce profound changes in the function of neighboring HC in coculture. In our model, coculture of either KC (2) or peritoneal macrophages (Mφ)(3) with HC normally promotes HC protein synthesis ([(3)H]leucine incorporation). The addition of LPS or killed Escherichia colt to such cocultures induces a profound decrease in HC protein synthesis, as well as qualitative changes ([(35)S]methionine, SDS-gel electrophoresis) in protein synthesis ...
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I need an antibody(ies) to stain canine Kupffer cells (macrophages) in liver. Have no knowledge of what crossreact to dog or a specific antibody for these cells. I am a mouse person, and need an answer for a bigger critter. I also would like a minirerun on using EDTA for antigen retrieval including MW, HIER, molarities, buffers, times. Thanks Gayle Callis ...
The use of monoclonal antibodies (mAbs) as therapeutic tools has increased dramatically in the last decade and is now one of the mainstream strategies to treat cancer. Nonetheless, it is still not completely understood how mAbs mediate tumor cell elimination or the effector cells that are involved. Using intravital microscopy, we found that antibody-dependent phagocytosis (ADPh) by macrophages is a prominent mechanism for removal of tumor cells from the circulation in a murine tumor cell opsonization model. Tumor cells were rapidly recognized and arrested by liver macrophages (Kupffer cells). In the absence of mAbs, Kupffer cells sampled tumor cells; however, this sampling was not sufficient for elimination. By contrast, antitumor mAb treatment resulted in rapid phagocytosis of tumor cells by Kupffer cells that was dependent on the high-affinity IgG-binding Fc receptor (FcγRI) and the low-affinity IgG-binding Fc receptor (FcγRIV). Uptake and intracellular degradation were independent of ...
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Human Hepatic Sinusoidal Endothelial Cell Genomic DNA https://www.sciencepro.com.br/produtos/sc-5009 https://www.sciencepro.com.br/@@site-logo/logo-novo.png ...
They are chemically dismantled, tagged and sent off by the enzyme systems for elimination.. Kupffer cells are large specialized macrophages (white blood cells) which phagocytize (eat) bacteria, endotoxins, antigen-antibody complexes and other liver poisons. This makes the liver an important immune system organ. These cells chew up most of the larger particles that enter the liver. However, they produce dangerous oxidative free radicals as a by-product of this process, and the liver requires a sufficient supply of protective antioxidants to neutralize them.. The livers cytochrome P450 system works on complex chemicals. As substances such as hormones, drugs, alcohol, carcinogens, pesticides and inflammatory chemicals like histamine enter the system, enzymes oxidize and break down the intruders (a process called phase I detoxification). After that, the liver chemically tags and changes the breakdown products so that they can be excreted (called phase II detoxification). This process also results ...
The course of the increased number of liver macrophages and the origin of these cells were studied after intravenous stimulation by zymosan, stilboestrol, or corynebacterium. The macrophages were isolated by digestion of the liver with pronase and DNAase. Zymosan doubled the number of liver macropha …
TY - JOUR. T1 - Characterization of Distinct Subpopulations of Hepatic Macrophages in HFD/Obese Mice. AU - Morinaga, Hidetaka. AU - Mayoral, Rafael. AU - Heinrichsdorff, Jan. AU - Osborn, Olivia. AU - Franck, Niclas. AU - Hah, Nasun. AU - Walenta, Evelyn. AU - Bandyopadhyay, Gautam. AU - Pessentheiner, Ariane. AU - Chi, Tyler. AU - Chung, Heekyung. AU - Bogner-Strauß, Juliane Gertrude. AU - Evans, Ronald M.. AU - Olefsky, Jerrold M.. AU - Oh, Da Young. PY - 2015. Y1 - 2015. M3 - Article. SP - 1120. EP - 1130. JO - Diabetes. JF - Diabetes. SN - 0012-1797. ER - ...
This specimen comes from an animal which was injected intravenously with a suspension of carbon particles. These particles are scavenged by macrophages, most notably by those in the liver which are called Kupffer cells., whose cytoplasm becomes packed with black carbon particles.. In the absence of such experimental demonstration, Kupffer cells can be recognized by their oval nuclei closely associated with sinusoidal spaces.. ...
Principal Investigator:KAMEI Takafumi, Project Period (FY):1995 - 1996, Research Category:Grant-in-Aid for Scientific Research (C), Section:一般, Research Field:General surgery
Kupffer cells have been isolated from transgenic mice carrying a thermolabile SV40 large tumor antigen under the H2Kb promoter (kind gift of D. Kioussis, NIMR, London). The cells grow with Interferon-gamma at 33oC, at which temperature the promoter is turned on and the SV40T Ag is active. They differentiate at 39oC. These cells are now being characterised: cytokine and NO liberation is stimulated, surface receptors are assessed at the mRNA and protein level, and phagocytosis and uptake of bacterial components are being measured. Results will be compared with the functional characteristics of primary Kupffer cells isolated from normal mice (see also 3R project 73-00) Conclusions and Relevance for 3R ...
The Nutshell Putative Gay Genes Identified, Questioned A genomic interrogation of homosexuality turns up speculative links between genetic elements and sexual orientation, but researchers say the study is too small to be significant. ...
Sigma-Aldrich offers abstracts and full-text articles by [Fang Zheng, Nick Devoogdt, Amanda Sparkes, Yannick Morias, Chloé Abels, Benoit Stijlemans, Tony Lahoutte, Serge Muyldermans, Patrick De Baetselier, Steve Schoonooghe, Alain Beschin, Geert Raes].
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Hepatic sinusoids are lined by LSEC BBuy Kupffer cells as well as dendritic cells, which interact either on the luminal or the basolateral side with LSEC (Figure 2. 114.
3D InSight™ Human Liver Toxicology Models Production lead time: 1 week Catalog # Description MT-02-302-04 3D InSight™ Human Liver Microtissues (multi-donor hepatocytes, co-culture with Kupffer cells and liver endothelial cells), 96x Order by Ships on: US Ships on: EU Aug 24 Sep 1 Sep 2 Sep 7 Sep 15 Sep 16 Sep 21 Sep
CELLINKs Liver Tissue Model Kit is your complete solution for generating and analyzing liver tissue models. The kit comes with bioinks that accommodate multiple cell types including hepatocytes, stellate cells, Kupffer cells and endothelial cells, giving you the ability to tailor each model to your needs. Once your model is generated, the kits antibodies enable you to directly analyze cells using key markers of liver functionality. Bioprinted liver tissue models organize cells spatially within a niche environment, providing the physiological mimicry necessary to enable the liver cells natural metabolism. Bioinks encapsulate the cells and mimic in vivo boundaries, enabling authentic dose and uptake responses and facilitating relevant drug screening.
recognizes most macrophages (including resident peritoneal and activated macrophages), Kupffer cells, but only about 35% of alveolar macrophages; also labels dendritic cells extensively, granulocytes and cells with the morphology of microglia in brain. ...
TY - JOUR. T1 - Tumor necrosis factor-α and interleukin-1β production by human fetal Kupffer cells. AU - Kutteh, William H.. AU - Rainey, William E.. AU - Beutler, Bruce. AU - Carr, Bruce R.. PY - 1991/7. Y1 - 1991/7. N2 - This study describes the isolation and characterization of human fetal Kupffer cells. We demonstrated that these cells have the potential to respond to cytokines and lipopolysaccharide with an increased production of tumor necrosis factor-α and interleukin-1β. Kupffer cells were characterized by: (1) morphologic characteristics after adherence to plastic, (2) staining for α-naphthyl acetate esterase, (3) immunofluorescence with monoclonal antibodies, and (4) phagocytosis of latex beads. More than 90% of the adherent cells were identified as macrophages. Kupffer cells cultured with lipopolysaccharide were able to produce interleukin-1β and tumor necrosis factor-α in a time- and dose-dependent fashion and maximal secretion was observed with the use of 10 μg of ...
Purpose: Liver ischemia reperfusion injury (IRI) remains a clinical problem associated with both surgical and non-surgical settings. Innate immune-dominated inflammation drives the pathogenesis of liver injuries. Although the activation of liver inflammation by IR have been studied extensively, few has been focused on the inflammation resolution in the disease process.. *Methods: In a murine liver partial warm ischemia model, we characterized the inflammation resolution during IR at histological, cellular and molecular levels. The role of Kupffer cells (KCs) was determined by clodronate-liposome (CL)-mediated depletion, and their functional mechanisms were explored by the inhibition of KC efferocytosis via TIM-4 blocking Abs, during the recovery stage of liver IRI (three doses at 24h, day 3 and day5 post reperfusion).. *Results: The restoration of liver homeostasis from a 90 min ischemia lasts for 7 days, as defined by: (i) repair of hepatocellular damage, (ii) clearance of infiltrating ...
Platelets play a critical role in atherogenesis and thrombosis-mediated myocardial ischemia, processes that are accelerated in diabetes. Whether hyperglycemia promotes platelet production and whether enhanced platelet production contributes to enhanced atherothrombosis remains unknown. Here we found that in response to hyperglycemia, neutrophil-derived S100 calcium-binding proteins A8/A9 (S100A8/A9) interact with the receptor for advanced glycation end products (RAGE) on hepatic Kupffer cells, resulting in increased production of IL-6, a pleiotropic cytokine that is implicated in inflammatory thrombocytosis. IL-6 acts on hepatocytes to enhance the production of thrombopoietin, which in turn interacts with its cognate receptor c-MPL on megakaryocytes and bone marrow progenitor cells to promote their expansion and proliferation, resulting in reticulated thrombocytosis. Lowering blood glucose using a sodium-glucose cotransporter 2 inhibitor (dapagliflozin), depleting neutrophils or Kupffer cells, ...
Platelets play a critical role in atherogenesis and thrombosis-mediated myocardial ischemia, processes that are accelerated in diabetes. Whether hyperglycemia promotes platelet production and whether enhanced platelet production contributes to enhanced atherothrombosis remains unknown. Here we found that in response to hyperglycemia, neutrophil-derived S100 calcium-binding proteins A8/A9 (S100A8/A9) interact with the receptor for advanced glycation end products (RAGE) on hepatic Kupffer cells, resulting in increased production of IL-6, a pleiotropic cytokine that is implicated in inflammatory thrombocytosis. IL-6 acts on hepatocytes to enhance the production of thrombopoietin, which in turn interacts with its cognate receptor c-MPL on megakaryocytes and bone marrow progenitor cells to promote their expansion and proliferation, resulting in reticulated thrombocytosis. Lowering blood glucose using a sodium-glucose cotransporter 2 inhibitor (dapagliflozin), depleting neutrophils or Kupffer cells, ...
This report concerns the phagocytic behavior of the Kupffer cells in the rat during hemorrhagic studies by quantitative technics. The rate of clearance (measured by colloidal carbon) was suppressed by 50 per cent after 3 hours. Efficiency of clearance (measured by uptake of radioisotope labeled albumin and chromium phosphate) was likewise markedly below normal. This factor was not due to reduced blood flow through the liver, as evidenced by measurements made 1 hour after blood replacement. Histologic evidence suggests two possibilities: a localized impairment of Kupffer cell activity in the area about the central veins of the lobule, or an abnormal circulation through preferential pathways restricted to the periphery of the liver lobule.. ...
TY - JOUR. T1 - Impaired hepatic bacterial clearance is reversed by surgical relief of obstructive jaundice. AU - Katz, Schmuel. AU - Yang, Rong. AU - Rodefeld, Mark. AU - Folkening, Walter J.. AU - Grosfeld, Jay L.. PY - 1991. Y1 - 1991. N2 - Sepsis is a major cause of morbidity and mortality in infants with cholestatic jaundice. Previous studies have shown that biliary obstruction in rats causes a significant decrease in hepatic phagocytosis of viable Escherichia coli. This study tests this hypothesis and further evaluates whether the impaired function of the reticuloendothelial system of the liver (Kupffer cells) can be reversed by the relief of the biliary obstruction. Male Sprague-Dawley rats (weighing 140 to 150 g) were placed in three groups. Group I (n = 10) consisted of sham-operated controls. In Group II (n = 30), ligation and division of distal common bile duct (CDL) was performed. Group III (n = 30) underwent choledochoduodenostomy 2 weeks following ligation and division of common ...
TY - JOUR. T1 - Kupffer cell transplantation in mice for elucidating monocyte/macrophage biology and for potential in cell or gene therapy. AU - Merlin, Simone. AU - Bhargava, Kuldeep K.. AU - Ranaldo, Gabriella. AU - Zanolini, Diego. AU - Palestro, Christopher J.. AU - Santambrogio, Laura. AU - Prat, Maria. AU - Follenzi, Antonia. AU - Gupta, Sanjeev. N1 - Funding Information: Supported in part by NIH grants R01 DK071111, R01 DK088561, P30 DK41296, and P30 CA13330 (S.G.) and Ricerca Sanitaria Finalizzata della Regione Piemonte, Progetti di Rilevante Interesse Nazionale (Project of Significant National Interest; PRIN) 2008 from Italian Ministry of Education, GGP09280 by Telethon Foundation Italy, and European Research Council (ERC) startup grant 261178 (A.F.). Publisher Copyright: © Copyright 2016 American Society for Investigative Pathology. Published by Elsevier Inc. All rights reserved.. PY - 2016/3/1. Y1 - 2016/3/1. N2 - Kupffer cells (KC) play major roles in immunity and tissue injury or ...
The effects of estriol on oxygen uptake, glucose release, lactate and pyruvate production, beta-hydroxybutyrate and acetoacetate production in perfused rat liver as well as, carbon uptake in rat liver and intracellular calcium in isolated Kupffer cells were investigated. Basal oxygen consumption of perfused liver increased significantly in estriol or ethanol-treated rats. But these increased effects were blocked by gadolinium chloride pretreatment. In a metabolic study, pretreatment with estriol resulted in a decrease in glucose production and in glycolysis while an increase in ketogenesis. A more oxidized redox state of the mitochondria was indicated by increased ratios of perfusate [lactate]/[pyruvate] and decreased ratios of perfusate [beta-hydroxybutyrate]/[acetoacetate]. Carbon uptake of Kupffer-cell increased significantly in estriol-treated rats. But these increased uptake were not shown in rats pre-treated by gadolinium chloride blocking phagocytosis. In isolated Kupffer cells from ...
To quantitatively determine the AuNP blood and tumor pharmacokinetic profiles, mice were killed at 2, 6, 12, and 24 h after nanoparticle injection. Blood was collected via cardiac puncture using a 1-mL syringe equipped with a 25-gauge needle. The harvested organs included lungs, heart, liver, spleen, stomach, intestines, kidneys, axillary lymph nodes, brachial lymph nodes, inguinal lymph nodes, and the tumor. A skin sample was also taken from the animals posterior thoracic cavity. Average mass of the tumors resected from each mouse tumor model can be found in SI Appendix, Table S2. Organs and blood samples were weighed and placed into glass culture tubes. In addition to organ samples, a portion of or the total administered AuNP dose was also added to a separate tube. To the samples, 800 µL of concentrated nitric acid [69% (wt/vol)] was added. For regional lymph nodes, this volume was reduced to 200 µL of nitric acid. An acid control sample containing solely nitric acid was also prepared in ...
Background: Numerous studies in rats and a few other mammalian species, including man, have shown that the sinusoidal cells constitute an important part of liver function. In the pig, however, which is frequently used in studies on liver transplantation and liver failure models, our knowledge about the function of hepatic sinusoidal cells is scarce. We have explored the scavenger function of pig liver sinusoidal endothelial cells (LSEC), a cell type that in other mammals performs vital elimination of an array of waste macromolecules from the circulation. Results: 125I-macromolecules known to be cleared in the rat via the scavenger and mannose receptors were rapidly removed from the pig circulation, 50% of the injected dose being removed within the first 2-5 min following injection. Fluorescently labeled microbeads (2 μm in diameter) used to probe phagocytosis accumulated in Kupffer cells only, whereas fluorescently labeled soluble macromolecular ligands for the mannose and scavenger receptors ...
5) Institute of Clinical and Experimental Immunology, Russian Academy of Medical Sciences, Novosibirsk, Russia.. Address for correspondence: T.A. Korolenko, Laboratory of Cellular Biochemistry, Institute of Physiology, Russian Academy of Medical Sciences, 4 Timakov Street, Novosibirsk 630117, Russia.. Summary: A high dose of acetaminophen (AAP; 1000 mg/kg, single, p.o) administered to rats induced acute liver failure with centrolobular degeneration and necrosis. We studied the role of hepatic macrophages (Kupffer cells) in the modulation of the acetaminophen-induced damage to the liver. Ukrain, which is known to possess immunomodulatory characteristics, has been shown to exhibit a positive hepatoprotective effect in human viral hepatitis C, significantly preventing liver cell injury. The selective inhibitor of Kupffer cells, gadolinium chloride (GdCI3, 7.5 mg/kg i.v, administered 24 h before AAP), and the macrophage stimulator carboxymethylated (1 -, 3)-beta-D-glucan (CMG; 25 mg/kg i.p., ...
UC San Diego researchers investigated how a type of immune cell called a macrophage becomes specialized to the liver. Their study sets the stage for understanding how macrophage specialization gets disrupted by - or contributes to - liver disease.
Convert between mass and molar concentrations of Gadolinium chloride using its molecular weight. Materials mass and molar concentrations calculator
Results We demonstrate an expansion of prorestorative MerTK+HLA-DRhigh cells in circulatory/tissue compartments of ALF patients (Fig.A). Compared to WT mice which have an increase of MerTK+MHCIIhigh macrophages during the resolution phase in ALF (Fig.B), APAP-treated Mer−/− mice exhibit persistent liver injury and inflammation, characterised by a decreased proportion of resident Kupffer cells (KC) and increased number of neutrophils (Fig.C). Both in vitro and in APAP-treated mice (Fig.D), SLPI reprograms macrophages towards resolution responses through induction of a MerTK+HLA-DRhigh phenotype that promotes neutrophil apoptosis and their subsequent clearance. ...
Principal Investigator:MATSUMOTO Kazunori, Project Period (FY):1991 - 1992, Research Category:Grant-in-Aid for General Scientific Research (C), Research Field:Gastroenterology
Scientists from TWINCORE have now published new insights on the processes involved in liver inflammation in the Journal of Hepatology: Type I interferons, on the one hand, limit viral replication and thereby help the immune cells to control the viral pathogen. On the other hand, type I interferons delay the regeneration of immune cells, which are important to adjust and maintain the immune balance within the liver during acute inflammation.
TY - JOUR. T1 - Expression and regulation of leukotriene-synthesis enzymes in rat liver cells. AU - Shimada, Kazuo. AU - Navarro, Javier. AU - Goeger, Douglas E.. AU - Mustafa, Shamimunisa B.. AU - Weigel, Paul H.. AU - Weinman, Steven A.. PY - 1998. Y1 - 1998. N2 - The liver plays a major role in metabolism and elimination of leukotrienes (LT). It produces cysteinyl leukotrienes (cLT), and cLT have been implicated in hepatocellular toxicity in several models of lipopolysaccharide (LPS)associated liver injury. However, the liver cell types responsible for cLT production are poorly defined, and the expression of the LT-synthesis enzymes, 5-lipoxygenase (5-LO) and LTC4 synthase (LTC4- S), in liver cells has never been demonstrated. The aim of the present study was to examine the ability of rat liver cells to produce cLT by determining whether hepatocytes, Kupffer cells, and sinusoidal endothelial cells express mRNA and enzyme activities of the LT-synthesis enzymes and whether expression is altered ...
This is a xanthous compound or pigment formed by the dislocation of hemoglobin. This originates from the dislocation of worn out, old or damaged ruddy blood cells. The aging blood cells are taken up and destroyed macrophages ( Kupffer cells ) of the phagocytic system ( which are located chiefly in the lien and in the liver. During the dislocation of hemoglobin into haem and hematohiston, the hematohiston is farther degraded to organize new proteins and the heme portion signifiers bilverdin. In the Kupffer cells, the bilverdin is converted to bilirubin by the usage of enzymes. The hematoidin is so released into the plasma where it binds to albumin and go an unconjugated hematoidin.. The unconjugated or free hematoidin enters the hepatocytes and after blending with glucuronic acid, it becomes a conjugated hematoidin. This conjugated hematoidin is H2O soluble and hence, soluble in gall, hence if the escape of gall is interrupted, conjugated hematoidin will impact the coloring material of the piss, ...
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Sigma-Aldrich offers abstracts and full-text articles by [Simone Merlin, Kuldeep K Bhargava, Gabriella Ranaldo, Diego Zanolini, Christopher J Palestro, Laura Santambrogio, Maria Prat, Antonia Follenzi, Sanjeev Gupta].
Tumor markers play an important role in the identification of human malignancies. It has been shown that the carcinoembryonic antigen (CEA, CEACAM5) is a promoter of metastasis in epithelial cancers that is widely used as a clinical marker. The aim of this study is to elucidate the network of genes that are involved in the CEA-induced liver metastasis. Previously, we have shown that CEA is accumulated in the lungs and livers of rats by interacting with their macrophages. We identified and cloned a new gene (CEAR) for the CEA-binding protein, which is located on the surface of fixed liver macrophages, Kupffer cells (Bajenova et al, 2001). It has been shown that the interaction of CEA and CEAR proteins increases the production of IL-1, IL-10, IL-6, TNF-α cytokines (Thomas et al, 2011). This interaction changes the expression of liver adhesion molecules that enhances the survival of cancer cells to the liver. We also suggested that CEA synthesis by cancer cells may influence the E-cadherin adhesion
Hepatitis C virus (HCV) is the leading cause of liver disease. HCV productively infects hepatocytes to impart liver inflammation and progressive tissue damage leading to fibrosis and cirrhosis. These processes underlie liver dysfunction and are thought to drive the onset of liver cancer. However, the molecular mechanism(s) by which HCV confers hepatic inflammation are not defined. Now, there is growing evidence that liver sinusoidal endothelial cells (LSEC) and Kupffer cells (KC), may play key roles in regulating immune responses and facilitating tolerance induction. These cells are playing a pivotal role in blood-borne virus clearance (>90%), leaving only a small fraction of infectious virus that escapes clearance in a manner peculiar to each individual pathogen. The biology of HCV, specifically regarding non-parenchymal liver cells, has been largely neglected. LSEC account for the 20% and KC for 15% of the hepatic cells, and are a unique organ-resident cell population with diverse functions, ...
Hepatitis C virus (HCV) is the leading cause of liver disease. HCV productively infects hepatocytes to impart liver inflammation and progressive tissue damage leading to fibrosis and cirrhosis. These processes underlie liver dysfunction and are thought to drive the onset of liver cancer. However, the molecular mechanism(s) by which HCV confers hepatic inflammation are not defined. Now, there is growing evidence that liver sinusoidal endothelial cells (LSEC) and Kupffer cells (KC), may play key roles in regulating immune responses and facilitating tolerance induction. These cells are playing a pivotal role in blood-borne virus clearance (>90%), leaving only a small fraction of infectious virus that escapes clearance in a manner peculiar to each individual pathogen. The biology of HCV, specifically regarding non-parenchymal liver cells, has been largely neglected. LSEC account for the 20% and KC for 15% of the hepatic cells, and are a unique organ-resident cell population with diverse functions, ...
Long-lived HIV-1 reservoirs that persist despite antiretroviral therapy (ART) are a major impediment to a cure for HIV-1. We examined whether human liver macrophages (LMs), the largest tissue macrophage population, comprise an HIV-1 reservoir. We purified LMs from liver explants and included treatment with a T cell immunotoxin to reduce T cells to 1% or less. LMs were purified from 9 HIV-1-infected persons, 8 of whom were on ART (range 8-140 months). Purified LMs were stimulated ex vivo and supernatants from 6 of 8 LMs from persons on ART transmitted infection. However, HIV-1 propagation from LMs was not sustained except in LMs from 1 person taking ART for less than 1 year. Bulk liver sequences matched LM-derived HIV-1 in 5 individuals. Additional in vitro experiments undertaken to quantify the decay of HIV-1-infected LMs from 3 healthy controls showed evidence of infection and viral release for prolonged durations (,170 days). Released HIV-1 propagated robustly in target cells, demonstrating ...
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Colour enhanced transmission electron micrograph (TEM) of a section of the liver showing hepatocytes with large nuclei and many mitochondria, small Kupffer cells, and sinusoids full of red blood cells. Magnification: x3,500 at 5 x 6.5 inches. - Stock Image C025/2732
The liver plays a key role in most metabolic processes, especially detoxification. The liver is a filter designed to remove toxic matter such as dead cells, microorganisms, chemicals, drugs and particulate debris from the bloodstream. The liver filter is called the sinusoidal system, and contains specialized cells known as Kupffer cells that are part of the white blood cell immune function. They make up 10% of liver weight, and function to ingest and break down toxic matter. Filtration of toxins is absolutely critical as the blood from the intestines contains high levels of bacterial waste, (endotoxins from the bowels), antigen-antibody complexes, and various toxic pollutants. When working properly, the liver clears 99% of the bacterial toxins during the first pass. However, when the liver is damaged, such as in alcoholics, the passage of toxins increases by over a factor of 10. This is similar if your intestines are too permeable, a condition known as leaky gut. Allergies (especially to ...
Macrophages undergo phenotypic changes in response to various environmental signals that can dramatically alter their function. The clinical relevance of this phenomenon stems in part from accumulating evidence that proinflammatory M1 polarization of macrophages contributes to metabolic impairment that is associated with obesity and diabetes (2,43). Thus, the deletion of M1 (classically activated) tissue macrophages normalizes sensitivity to insulin in obese mice (44,45), while, conversely, the reduction of M2 (alternatively activated) macrophages predisposes lean mice to the development of insulin resistance (13), implying a critical role for M2 polarization of macrophages in metabolic homeostasis. The current work suggests that in Kupffer cells, endothelial NO signaling maintains M2 polarization, whereas the effect of HFD feeding to reduce hepatic NO signaling reduces M2 and favors M1 polarization of these cells. This conclusion offers a feasible explanation for the findings that deficient NO ...
A very large part of intravenously administered nanoparticles are cleared through the liver. Within the liver, most nanoparticles are thought to be sequestered by macrophages (Kupffer cells). To achieve effective cell-specific targeting of drugs and non-viral gene delivery vectors, improved mechanistic…
The suppression of P450 enzymes and other DMEs by certain therapeutic proteins and the rise in the coadministration of therapeutic proteins with small-molecule drugs create the potential for clinical DDIs. We investigated a new in vitro method for evaluating therapeutic proteins as perpetrators of DDIs with small-molecule drugs (Fig. 1). The method consists of adding the therapeutic protein to human blood ex vivo to permit the release of cytokines from PBMCs followed by the preparation of plasma and an assessment of its effects on DME in human hepatocytes cocultured with Kupffer cells. Suppression of DME by certain therapeutic proteins commonly involves the release of cytokines from PBMCs (Morgan et al., 2002, 2008). Current procedures to assess therapeutic proteins as perpetrators of DDIs rely primarily on clinical studies because the predictive value of applying cytokines individually or in a limited combination to hepatocytes in vitro is not well established (Girish et al., 2011; Evers et ...
Oppblomstring av cyanobakterier (blågrønnalger) forekommer i ferskvann, brakkvann og saltvann. Disse organismene kan produsere flere typer hepato-, nevro- og nevrodegenererende toksiner.
... giant cells, Kupffer cells, and osteoclasts. This allows it to be used to distinguish diseases of otherwise similar appearance ... Kupffer cells, osteoclasts, monocytes, and activated microglia in rat tissues. In this species, it is expressed in most ... Kupffer cells, microglia). Human CD68 is a transmembrane glycoprotein, heavily glycosylated in its extracellular domain, with a ... "Tri-iodothyronine differentially induces Kupffer cell ED1/ED2 subpopulations". Molecular Aspects of Medicine. 25 (1-2): 183-90 ...
... from Kupffer Cells). or nitrous oxide (NO) and endothelin-1 (ET-1). ET-1 is the most potent vasoconstrictor under investigation ... The muscular pulmonary arteries become fibrotic and hypertrophy while the smaller arteries lose smooth muscle cells and their ... binds G-protein linked cell surface receptors that activate adenylate cyclase to relax vascular smooth muscle. Prostacyclin - ...
Kupffer cells are scattered between endothelial cells; they are part of the reticuloendothelial system and phagocytose spent ... A hepatocyte is a cell of the main parenchymal tissue of the liver. Hepatocytes make up 80% of the liver's mass. These cells ... In the first step, the liver is placed in an isotonic solution, in which calcium is removed to disrupt cell-cell tight ... The hepatocyte plates are one cell thick in mammals and two cells thick in the chicken. Sinusoids display a discontinuous, ...
They undergo schizogony in the hepatocytes and Kupffer cells. They then invade the nervous tissue of the rodent host, forming ...
Kawai, Y; Smedsrød, B; Elvevold, K; Wake, K (May 1998). "Uptake of lithium carmine by sinusoidal endothelial and Kupffer cells ... Anderson, CL (December 2015). "The liver sinusoidal endothelium reappears after being eclipsed by the Kupffer cell: a 20th ... The studies showed that the cell system that Aschoff described as RES in the liver were liver sinusoidal endothelial cells ( ... During the 1980s and 1990s some laboratories noted that specialized endothelial cells (called scavenger endothelial cells), but ...
... being occupied by Kupffer cells, hepatic stellate cells, endothelial cells and mesothelial cells, which are not exactly ... Main liver cells are hepatocytes, Kupffer cells, and hepatic stellate cells; each one with a specific function. Cytology is the ... The main function of the Kupffer cells is the destruction of old blood cells that go through the liver. In the perisinusoidal ... there are other cells that can be observed in a liver sample such as Kupffer cells (macrophages). The liver is the biggest ...
Andrzej Śródka, Ryszard W. Gryglewski, Wojciech Szczepański: Browicz or Kupffer Cells? Journal of Physiology and Pharmacology, ... and in 1898 was the first to correctly identify the liver's Kupffer cells as specialized macrophages. He also performed ...
Sunman JA, Hawke RL, LeCluyse EL, Kashuba AD (March 2004). "Kupffer cell-mediated IL-2 suppression of CYP3A activity in human ...
Hinglais N, Kazatchkine MD, Mandet C, Appay MD, Bariety J (November 1989). "Human liver Kupffer cells express CR1, CR3, and CR4 ... protects host cells from complement-mediated damage by regulating the activation of C3 convertases on host cell surfaces; ... The gene is expressed principally on erythrocytes, monocytes, neutrophils and B cells but is also present on some T lymphocytes ... Donius LR, Handy JM, Weis JJ, Weis JH (July 2013). "Optimal germinal center B cell activation and T-dependent antibody ...
... which causes osmotic lysis of the target cell. Kupffer cells and other macrophage cell types help clear complement-coated ... Host cells don't accumulate cell surface C3b (and the proteolytic fragment of C3b called iC3b) because this is prevented by the ... the ability of antibodies and phagocytic cells to clear microbes and damaged cells from an organism, promote inflammation, and ... In contrast, when the internal thioester of C3 reacts with a hydroxyl or amino group of a molecule on the surface of a cell or ...
Diminished organelle motion in murine Kupffer cells during the erythrocytic stage of malaria. J R Soc Interface. 2011 May 6;8( ... Am J Respir Cell Mol Biol. 2006; 35:415-23. Trujillo JR, Rogers R, Molina RM, Dangond F, McLane MF, Essex M, Brain JD. Non- ... Repeated mouse lung exposures to Stachybotrys chartarum shift immune response from type 1 to type 2. Am J Respir Cell Mol Biol ...
... is a protein expressed in Kupffer cells. It is a critical receptor for the ... Cell. 124 (5): 915-27. doi:10.1016/j.cell.2005.12.039. PMID 16530040. v t e. ...
"Kupffer cells are depleted with HIV immunodeficiency and partially recovered with antiretroviral immune reconstitution". AIDS. ... "Hepatitis C virus immune escape via exploitation of a hole in the T cell repertoire". Journal of Immunology. 181 (9): 6435-6446 ... "High-programmed death-1 levels on hepatitis C virus-specific T cells during acute infection are associated with viral ...
2001). "Heterogeneous RNA-binding protein M4 is a receptor for carcinoembryonic antigen in Kupffer cells". J. Biol. Chem. 276 ( ... analysis of its promoter indicates a region conveying cell type-specific expression". Mol. Cell. Biol. 10 (6): 2738-48. doi: ... Cell. Biol. 7 (9): 3221-30. doi:10.1128/MCB.7.9.3221. PMC 367958. PMID 3670312. Oikawa S, Nakazato H, Kosaki G (1987). "Primary ... Carcinoembryonic antigen-related cell adhesion molecule 5 (CEACAM5) also known as CD66e (Cluster of Differentiation 66e), is a ...
In the liver, Kupffer cells store excess iron from catabolism of heme from the breakdown of red blood cells. In bone marrow and ... The cells are primarily monocytes and macrophages, and they accumulate in lymph nodes and the spleen. The Kupffer cells of the ... Macrophages are diffusely scattered in the connective tissue and in liver (Kupffer cells), spleen and lymph nodes (sinus ... Formation of new red blood cells (RBCs) and white blood cells (WBCs). Destruction of senescent RBCs. Formation of plasma ...
During late phase the appearance is isoechoic or hypoechoic, due to lack of Kupffer cells.[citation needed] Malignant liver ... Systemic therapies are procedures based on the affinity of certain molecules to inhibit either tumor cell replication or ...
The Kupffer cells of the liver then phagocytose endotoxin, stimulating the release of TNF-α. TNF-α then triggers apoptotic ... Fatty change, or steatosis, is the accumulation of fatty acids in liver cells. These can be seen as fatty globules under the ... Subsequently, the higher levels of fatty acids signal the liver cells to compound it to glycerol to form triglycerides. These ... These factors cause inflammation, apoptosis and eventually fibrosis of liver cells. Why this occurs in only a few individuals ...
"The proinflammatory myeloid cell receptor TREM-1 controls Kupffer cell activation and development of hepatocellular carcinoma ... Collins FS, Rossant J, Wurst W (January 2007). "A mouse for all reasons". Cell. 128 (1): 9-13. doi:10.1016/j.cell.2006.12.018. ... Triggering receptor expressed on myeloid cells 1 (TREM-1) is a protein that in humans is encoded by the TREM1 gene. TREM-1 is a ... In granulocyte cells, C/EBPε was also shown to induce TREM-1 expression independently from inflammatory response. The ...
These individuals are likely to have liver and spleen iron overload, primarily in Kupffer cells and other macrophages. Because ... Iron accumulates preferentially in Kupffer cells, which are located in the liver, and serum ferritin increases; less iron is ... Iron in the bloodstream is then bound by transferrin, which carries the iron to target cells. Iron is stored in cells and blood ... More ferritin is produced to suppress oxidative cell damage, although the amount of ferritin that cells can accumulate is ...
... sinusoidal endothelial cells, and phagocytic Kupffer cells. Hepatic stellate cells are nonparenchymal cells found in the ... Histology, the study of microscopic anatomy, shows two major types of liver cell: parenchymal cells and nonparenchymal cells. ... Nonparenchymal cells constitute 40% of the total number of liver cells but only 6.5% of its volume. The liver sinusoids are ... Adv Anat Embryol Cell Biol. Advances in Anatomy Embryology and Cell Biology. 161. pp. iii-xiii, 1-151. doi:10.1007/978-3-642- ...
"Cell-surface arylsulfatase A and B on sinusoidal endothelial cells, hepatocytes, and Kupffer cells in mammalian livers". ... sinusoidal endothelial cells, and Kupffer cells in the liver, as well as in the apical membranes of normal and malignant ... Measurements in cystic fibrosis cell line IB3 and its derivative cell line C38, which has a functional CFTR, showed increased ... "Cell-bound IL-8 increases in bronchial epithelial cells after arylsulfatase B silencing due to sequestration with chondroitin-4 ...
In 1876, Karl Wilhelm von Kupffer described the properties of some liver cells now called Kupffer cells. In 1883, Hugo ...
... liver histology shows microvesicular steatosis and cholestasis with abundant iron accumulation in hepatocytes and Kupffer cells ...
The liver sinusoidal endothelium reappears after being eclipsed by the Kupffer cell: a 20th century biological delusion ... These cells (LSEC) take up other viruses and other particles in their capacity as the body's garbage dump, clearing small ... Beta 2-microglobulin-, CD8+ T-cell-deficient mice survive inoculation with high doses of vaccinia virus and exhibit altered IgG ... Antibody characterization and induction of superoxide production in a monocyte cell line. J Biol Chem. Sep 25;261(27):12856-64 ...
The protein is most prevalent in the kidney and the liver where it is found in Kupffer cells. STARD5 binds both cholesterol and ... Collins FS, Rossant J, Wurst W (January 2007). "A mouse for all reasons". Cell. 128 (1): 9-13. doi:10.1016/j.cell.2006.12.018. ... Increased levels of StarD5 increase free cholesterol in the cell. Cholesterol homeostasis is regulated, at least in part, by ... "START domain proteins and the intracellular trafficking of cholesterol in steroidogenic cells". Molecular and Cellular ...
Peripheral macrophage as well as liver sinusoidal endothelial cells and Kupffer cells have been implicated in this process, ... is found on many cells, including endothelial cells, smooth muscle, cells of the immune system[which?] from tissue such as lung ... a central cell surface receptor for S100/calgranulin polypeptides". Cell. 97 (7): 889-901. doi:10.1016/S0092-8674(00)80801-6. ... AGEs affect nearly every type of cell and molecule in the body and are thought to be one factor in aging and some age-related ...
2006). "The cachectic mediator proteolysis inducing factor activates NF-kappaB and STAT3 in human Kupffer cells and monocytes ... The N-terminal peptide, also known as diffusible survival evasion peptide, promotes neural cell survival under conditions of ... 1998). "Identification of a survival-promoting peptide in medium conditioned by oxidatively stressed cell lines of nervous ... 2006). "dermicidin expression in hepatic cells improves survival without N-glycosylation, but requires asparagine residues". Br ...
Kupffer cells produce IL-6 in the liver and present it to the hepatocytes. IL-6 is the major mediator for the hepatocytic ... Cortisol can enhance expression of IL-6 receptors in liver cells and induce IL-6-mediated production of APPs. Positive acute- ... In response to injury, local inflammatory cells (neutrophil granulocytes and macrophages) secrete a number of cytokines into ... the innate immune system by their ability to increase vascular permeability and act as chemotactic agents for phagocytic cells ...
I. In vitro and in vivo activation of lymphocytes by liver regeneration and the role of Kupffer cells in stimulation". European ... Antigen-presenting cells in newborns have a reduced capability to activate T cells. Also, T cells of a newborn proliferate ... There is also some evidence that cell surface receptors on B cells and macrophages may detect sex hormones in the system. The ... Suppression of primary M, G, and A plaque-forming cell responses in mouse spleen cell cultures by class-specific antibody to ...
I. In vitro and in vivo activation of lymphocytes by liver regeneration and the role of Kupffer cells in stimulation". European ... Beta cell - Beta cellscells) are a type of cell found in pancreatic islets that synthesize and secrete insulin. Beta cells ... White blood cell - White blood cells (WBCs), also called leukocytes or leucocytes, are the cells of the immune system that are ... All white blood cells are produced and derived from multipotent cells in the bone marrow known as hematopoietic stem cells. ...
當血液中的紅血球死亡,紅血球中血紅蛋白的血紅素會於肝臟的庫佛氏
... probably indirectly via Kupffer cells), which leads to eosinophilic degradation of these cells and to the release of cytokines ... The viruses infect, amongst others, monocytes, macrophages, and dendritic cells. They attach to the cell surfaces via specific ... After entering the host cell, the viral genome is replicated in the rough endoplasmic reticulum (ER) and in the so-called ... After transmission from a mosquito, the viruses replicate in the lymph nodes and infect dendritic cells in particular. From ...
Kaposi's sarcoma (KS) - Karnofsky score - killer T cells - KSHV - Kupffer cells LEdit. Langerhans cells - LAS - lentivirus - ... T suppressor cells - T4 cell - T4 cells (T-helper cells) - T8 cells - Tanner staging - TAT - TB - template - TeachAIDS - ... B-cell lymphoma - B cells - B lymphocytes (B cells) - bactericidal - bacteriostatic - bacterium - baculovirus - baseline - ... cells - CDC National Prevention Information Network (CDC-NPIN) - cell lines - cell-mediated immunity (CMI) - cellular immunity ...
... which become known as Kupffer cells. These cells still serve a role in the immune system. ... T cells: *CD4+ helper T cells: T cells displaying co-receptor CD4 are known as CD4+ T cells. These cells have T-cell receptors ... B cells: releases antibodies and assists activation of T cells. *T cells: *CD4+ Th (T helper) cells: activate and regulate T ... Natural killer cells: virus-infected and tumor cells.. Deeply staining, eccentric. NK-cells and cytotoxic (CD8+) T-cells. Years ...
Kupffer cells, and mast cells. These cells present receptors contained on the surface or within the cell, named pattern ... Rather, NK cells destroy compromised host cells, such as tumor cells or virus-infected cells, recognizing such cells by a ... Mast cells[edit]. Main article: Mast cell. Mast cells are a type of innate immune cell that reside in connective tissue and in ... Natural killer cells[edit]. Main article: Natural killer cell. Natural killer cells (NK cells) are a component of the innate ...
These include the various cells of the gastric glands, taste cells, pancreatic duct cells, enterocytes and microfold cells. ... Another product is iron, which is used in the formation of new blood cells in the bone marrow.[5] Medicine treats the spleen ... The parietal cells in the fundus of the stomach, produce a glycoprotein called intrinsic factor which is essential for the ... The spleen is the largest lymphoid organ in the body but has other functions.[23] It breaks down both red and white blood cells ...
... they regulate other immune cell functions (e.g., CD4+ T cell, dendritic cell, B cell, mast cell, neutrophil, and basophil ... Kupffer cells. *Alveolar macrophage. *Microglia. *Osteoclasts. *Epithelioid cells. *giant cells *Langhans giant cells, Foreign- ... Mast cells[edit]. See article: Mast cell. Mast cells are a type of granulocyte that are present in tissues;[3] they mediate ... Basophils are one of the least abundant cells in bone marrow and blood (occurring at less than two percent of all cells). Like ...
"Cloning of a new cytokine that induces IFN-gamma production by T cells". Department of Bacteriology, Hyogo College of Medicine ... IL-18 umumnya merupakan sekresi makrofaga dan sel Kupffer.[2] Peningkatan ekspresi IL-18 ditemukan pada beberapa jenis kanker, ...
An alternative source of beta cells, such insulin-producing cells derived from adult stem cells or progenitor cells would ... activates beta cells and inhibits alpha cells. *Glycogen/Glucagon: activates alpha cells which activates beta cells and delta ... PP cells (gamma cells or F cells) producing pancreatic polypeptide (,5%). It has been recognized that the cytoarchitecture of ... In rat islets, endocrine cell subsets are distributed as follows:[6] *Alpha cells producing glucagon (20% of total islet cells) ...
Kupffer cells and sinusoidal endothelial cells from rat liver". Hepatology. 11 (2): 199-204. doi:10.1002/hep.1840110207. PMID ... such as cell aggregation, migration, proliferation and activation; cell-cell and cell-substrate adhesion; endocytosis of HA, ... Cell migrationEdit. Cell migration is essential for the formation of granulation tissue.[26] The early stage of granulation ... Cell receptors for hyaluronic acidEdit. So far, cell receptors that have been identified for HA fall into three main groups: ...
This promotes further liver damage.[35] Non-parenchymal cells such as Kupffer cells, fat storing stellate cells, and leukocytes ... such as stem cell-derived hepatocyte-like cells, that are capable of detecting toxicity early in the drug development process.[ ... This is the most common type of drug-induced liver cell necrosis where the injury is largely confined to a particular zone of ... Greenhough S, Hay DC (2012). "Stem Cell-Based Toxicity Screening: Recent Advances in Hepatocyte Generation". Pharm Med. 26 (2 ...
... which causes osmotic lysis of the target cell. Kupffer cells and other macrophage cell types help clear complement-coated ... Host cells don't accumulate cell surface C3b (and the proteolytic fragment of C3b called iC3b) because this is prevented by the ... the ability of antibodies and phagocytic cells to clear microbes and damaged cells from an organism, promotes inflammation, and ... This creates a hole or pore in the membrane that can kill or damage the pathogen or cell.[citation needed] ...
Kupffer cells. *Alveolar macrophage. *Microglia. *Osteoclasts. *Epithelioid cells. *giant cells *Langhans giant cells, Foreign- ... Blood plasma is a yellowish coloured liquid component of blood that normally holds the blood cells in whole blood in suspension ... this makes plasma the extracellular matrix of blood cells. It makes up about 55% of the body's total blood volume.[1] It is the ... When donating whole blood or packed red blood cell (PRBC) transfusions, O- is the most desirable and is considered a "universal ...
當血液中的紅血球死亡,紅血球中血紅蛋白的血紅素會於肝臟的庫佛氏
Histiosit " Sel Kupffer " Alveolar " Pleural " Peritoneal " Langerhans's cell. Radang. DC. Mieloid. Plasmasitoid. ...
Any ANC , 1500 cells / mm3 is considered neutropenia, but ,500 cells / mm3 is considered severe.[46] There is also new research ... HSC=Hematopoietic stem cell, Progenitor=Progenitor cell, L-blast=lymphoblast, Lymphocyte, Mo-blast=Monoblast, Monocyte, ... Whereas basophilic white blood cells stain dark blue and eosinophilic white blood cells stain bright red, neutrophils stain a ... Reference ranges for blood tests of white blood cells, comparing neutrophil amount (shown in pink) with that of other cells ...
... including Kupffer cells), and some dendritic cells.[10] It is composed of two extracellular Ig-like domains, and is a member of ... Langerhans cells Phagocytosis. Degranulation (eosinophils) FcγRIIB1 (CD32) IgG Low (Kd , 10−7 M) B Cells. Mast cells No ... Mast cells. Follicular dendritic cells Induction of microbe killing FcεRI IgE High (Kd ~ 10−10 M) Mast cells. Eosinophils. ... On NK cellsEdit. The Fc receptor on NK cells recognize IgG that is bound to the surface of a pathogen-infected target cell and ...
I. In vitro and in vivo activation of lymphocytes by liver regeneration and the role of Kupffer cells in stimulation". European ... Antigen-presenting cells in newborns have a reduced capability to activate T cells. Also, T cells of a newborn proliferate ... Suppression of primary M, G, and A plaque-forming cell responses in mouse spleen cell cultures by class-specific antibody to ... There is also some evidence that cell surface receptors on B cells and macrophages may detect sex hormones in the system.[27] ...
... such as fat cells, muscle cells, liver cells (and also in the endothelial cells, Kupffer cells and stellate cells of the liver ... cell membrane. • presynaptic membrane. • mitochondrion. • mitochondrial outer membrane. • cell projection. • integral component ... "Cell. 167 (3): 750-762.e14. doi:10.1016/j.cell.2016.10.004. PMC 5322940. PMID 27768894.. ... In the retina, they are expressed in the photoreceptors, inner plexiform, outer plexiform, bipolar cells, ganglion cells, and ...
"Redox regulation of thyroid hormone-induced Kupffer cell-dependent IkappaB-alpha phosphorylation in relation to inducible ... "Hepatic Stellate Cell in Fibrosis: Stellate Cell Activation". Division of Liver Diseases, Mount Sinai School of Medicine; Scott ... "Stem cells, cell transplantation and liver repopulation". Marion Bessin Liver Research Center, Division of Hepatology, ... "Hepatic progenitor cells, stem cells, and AFP expression in models of liver injury". Division of Radiooncology, Deutsches ...
"Redox regulation of thyroid hormone-induced Kupffer cell-dependent IkappaB-alpha phosphorylation in relation to inducible ... "Hepatic Stellate Cell in Fibrosis: Stellate Cell Activation". Division of Liver Diseases, Mount Sinai School of Medicine; Scott ... "Stem cells, cell transplantation and liver repopulation". Marion Bessin Liver Research Center, Division of Hepatology, ... "Hepatic progenitor cells, stem cells, and AFP expression in models of liver injury". Division of Radiooncology, Deutsches ...
Liver biopsy findings will generally show a bright yellow-orange color, enlarged, lipid-laden hepatocytes and Kupffer cells, ... Some children with LAL-D have had an experimental therapy called hematopoietic stem cell transplantation (HSCT), also known as ... and neuropsychological outcome of hematopoietic cell transplantation for Wolman disease". Bone Marrow Transplantation. 43 (1): ... eventually leading to life-threatening problems such as extremely low levels of circulating red blood cells (severe anemia), ...
"Cell. 130 (6): 1005-18. doi:10.1016/j.cell.2007.07.020. PMC 2586609 . PMID 17889646.. ... hspb7 also acts in the downregulation of Kupffer vesicles which is responsible for regulation of left-right asymmetry of heart ... Tumor cells usually express only a few neo-antigens, which can be targeted by immune system and also not all tumor cells ... Also isolated HSPs from tumour cells are able to act as a specific anti-tumour vaccine by themselves.[28][26] Tumour cells ...
It also adversely affects the function of Kupffer cells, which are specialized macrophage cells that assist in preventing ... Bile is formed in the liver by hepatocytes (liver cells) and excreted into the common hepatic duct. Part of the bile is stored ... In the early stages, however, pressure on the liver cells may be the main feature and the tests will resemble those in ... Routine blood tests show features of acute inflammation (raised white blood cell count and elevated C-reactive protein level), ...
Inhibition of Kupffer cell functions as an explanation for the hepatoprotective properties of silibinin. Dehmlow, Carola; ... In vitro protective effects of salvianolic acid B on primary hepatocytes and hepatic stellate cells Journal of ...
Dendritic cells (Langerhans cells, Follicular dendritic cells). Monocytes/Macrophages (Histiocytes, Kupffer cells, Langhans ... T cells: Cytotoxic CD8+, Helper CD4+/Regulatory, γδ, Natural Killer T cell. B cells: Plasma, Memory. Natural killer cells ( ... Natural killer cells: cell a liban dang virus ampong tumor cell.. malalam mikukulayan, e makasentru. bukud mung NK-cells [5]. ... Deng γδ T cell atin lang alternatibung T cell receptor, e anti kareng CD4+ and CD8+ αβ T cell, at kayawig do reng helper T cell ...
Investigations concerning Kupffer cells are hampered because in humans, Kupffer cells are only accessible for ... Once a T cell has recognized its particular antigen on the surface of an aberrant cell, the T cell becomes an activated ... The processed antigen is then presented in MHCII on the surface of the B-cell. T cells that express the T cell receptor which ... Macrophages are a type of repairing cell that devour dead cells and pathogens, and trigger other immune cells to respond to ...
Kupffer cells. *Alveolar macrophage. *Microglia. *Osteoclasts. *Epithelioid cells. *giant cells *Langhans giant cells, Foreign- ... "Distinguishing mast cell and granulocyte differentiation at the single-cell level". Cell Stem Cell. 6 (4): 361-8. doi:10.1016/j ... This article is about a type of blood cell. For the endocrine cell of the anterior pituitary, see basophil cell. ... Both cell types store histamine, a chemical that is secreted by the cells when stimulated. However, they arise from different ...
... that dispose of old blood cells and particulate matter. Kupffer cells, named after Karl Wilhelm von Kupffer (1829-1902), are ... Kupffer cells Specialized macrophages that dispose of old blood cells and particulate matter. Kupffer cells, named after Karl ... Kupffer cells (kuup-fer) pl. n. phagocytic cells that line the sinusoids of the liver (see macrophage). They are particularly ... Kupffer cells A Dictionary of Biology © A Dictionary of Biology 2004, originally published by Oxford University Press 2004. ...
Kupffer cells, also known as stellate macrophages and Kupffer-Browicz cells, are specialized cells localized in liver within ... The amoeboid shaped Kupffer cells are attached to sinusoidal endothelial cells. The surface of Kupffer cells contain microvilli ... The average life of a Kupffer cell is around 3.8 days. The primary function of the Kupffer cell is to remove foreign debris and ... October 2019). "Stellate Cells, Hepatocytes, and Endothelial Cells Imprint the Kupffer Cell Identity on Monocytes Colonizing ...
Kupffer cell definition at Dictionary.com, a free online dictionary with pronunciation, synonyms and translation. Look it up ...
Phenotypic, developmental, and functional aspects of these cells in... ... Kupffer cells (KC), the resident macrophages of the liver, represent the largest population of mononuclear phagocytes in the ... Wan J, Benkdane M, Teixeira-Clerc F, Bonnafous S, Louvet A, Lafdil F et al (2014) M2 Kupffer cells promote M1 Kupffer cell ... Song E, Chen J, Ouyang N, Wang M, Exton MS, Heemann U (2001) Kupffer cells of cirrhotic rat livers sensitize colon cancer cells ...
Depletion and repopulation of Kupffer cells in BALB/c mice. The number of Kupffer cells from fixed areas of tissue were ... that phagocytic Kupffer cells near the portal triads were depleted to a greater extent in comparison with the Kupffer cells ... Kupffer cells and nanoparticle tumor delivery. Anthony J. Tavares, Wilson Poon, Yi-Nan Zhang, Qin Dai, Rickvinder Besla, Ding ... Kupffer cells and nanoparticle tumor delivery. Anthony J. Tavares, Wilson Poon, Yi-Nan Zhang, Qin Dai, Rickvinder Besla, Ding ...
Depletion and repopulation of Kupffer cells in BALB/c mice. The number of Kupffer cells from fixed areas of tissue were ... Effect of removing Kupffer cells on nanoparticle tumor delivery.. Tavares AJ1,2, Poon W1,2, Zhang YN1,2, Dai Q1,2, Besla R3, ... near-complete depletion of Kupffer cells can be seen in the hepatic lobule shown in C. (G) Normalized percentage of Kupffer ... Clodronate liposomes are i.v. injected into the mouse via the tail vein to remove a portion of Kupffer cells within 48 h. Gold ...
Kupffer Cell Cell Organelle Microsomal Membrane Subcellular Organelle Residual Body These keywords were added by machine and ... Glauhann H. (1976) Phagocytosis and Intralysosomal Digestion of Subcellular Organelles by Kupffer Cells of Rat Liver. In: ... Phagocytosis and Intralysosomal Digestion of Subcellular Organelles by Kupffer Cells of Rat Liver. ... an experimental model was used in which cellular organelles were introduced to the lysosomal apparatus of Kupffer cells by ...
Kupffer cells (KC) constitute 80-90% of the tissue macrophages present in the body. They reside within the lumen of the liver ... Role of Kupffer cells in host defense and liver disease Liver Int. 2006 Dec;26(10):1175-86. doi: 10.1111/j.1478-3231.2006.01342 ... stellate cells, endothelial cells and other immune cells that traffic through the liver. Therefore, KC are intimately involved ... Kupffer cells (KC) constitute 80-90% of the tissue macrophages present in the body. They reside within the lumen of the liver ...
Cells were washed after 3 hours to remove dead cells, and fresh culture medium was added to incubate the cells overnight. ... STING-mediated inflammation in Kupffer cells contributes to progression of nonalcoholic steatohepatitis. Yongsheng Yu,1 Yu Liu, ... Depletion of Kupffer cells attenuates systemic insulin resistance, inflammation and improves liver autophagy in high-fat diet ... The mitochondrial DNA (mtDNA) from hepatocytes of HFD-fed mice induced TNF-α and IL-6 expression in cultured Kupffer cells (KCs ...
The mitochondrial DNA (mtDNA) from hepatocytes of HFD-fed mice induced TNF-α and IL-6 expression in cultured Kupffer cells (KCs ... STING-mediated inflammation in Kupffer cells contributes to progression of nonalcoholic steatohepatitis. ... STING-mediated inflammation in Kupffer cells contributes to progression of nonalcoholic steatohepatitis. ...
Taken together, we developed a series of nanobodies targeting multiple distinct recognition epitopes of the Kupffer cell- ... Taken together, we developed a series of nanobodies targeting multiple distinct recognition epitopes of the Kupffer cell- ... by a phage display screening building from lymphocytes isolated from an alpaca immunized with recombinant mouse Kupffer cell ... by a phage display screening building from lymphocytes isolated from an alpaca immunized with recombinant mouse Kupffer cell ...
Kupffer cells explanation free. What is Kupffer cells? Meaning of Kupffer cells medical term. What does Kupffer cells mean? ... Looking for online definition of Kupffer cells in the Medical Dictionary? ... Kupffer, Karl W. von, German anatomist, 1829-1902. Kupffer cell sarcoma. Kupffer cells - phagocytic cells of the mononuclear ... Related to Kupffer cells: Space of Disse. Kupf·fer cells. (kūpfĕr), phagocytic cells of the mononuclear phagocyte series found ...
On the other hand, type I interferons delay the regeneration of immune cells, which are important to adjust and maintain the ... limit viral replication and thereby help the immune cells to control the viral pathogen. ... Kupffer cells disappear after infection." The body replaces those lost Kupffer cells by scavenger cells, which develop from the ... Such cells are not "real" Kupffer cells, but they still take over similar tasks. Interestingly, this process is accelerated if ...
What is Kupffer cell? Meaning of Kupffer cell as a legal term. What does Kupffer cell mean in law? ... Definition of Kupffer cell in the Legal Dictionary - by Free online English dictionary and encyclopedia. ... Kupffer cell legal definition of Kupffer cell https://legal-dictionary.thefreedictionary.com/Kupffer+cell ... These findings confirm that the binding of endotoxin to its receptors on the Kupffer cells and the resulting Kupffer cell ...
Kupffer cells isolated from rats treated with ethanol for 4 weeks exhibit sensitization to LPS. It is likely that increased ... Studies with gut sterilization and Kupffer cell inactivation support the hypothesis that endotoxin and Kupffer cells are ... Kupffer cell sensitization by alcohol involves increased permeability to gut-derived endotoxin Alcohol Clin Exp Res. 2001 Jun; ... In isolated Kupffer cells from rats treated with ethanol for 4 weeks, CD14, LPS-induced intracellular Ca2+, and TNF-alpha all ...
Hepatocyte and Kupffer Cell Interactions. Hepatocyte and Kupffer Cell Interactions. by Ronald D. Curran, Timothy R. Billiar by ... The role of Kupffer cells and hepatoctyes in normal physiology, nonseptic pathological states, and in sepsis is examined. ... In each of the books chapters, the role of the Kupffer cell and hepatocyte interaction is placed in context with information ... Hepatocyte and Kupffer Cell Interactions presents a comprehensive discussion of historical and recent information regarding ...
However, some tumor cells evaded the Kupffer cells as metastases still occurred. Kupffer cells and other macrophages were found ... including tumor cells, from the liver. The role of Kupffer cells in tumor development is very important as Kupffer cells can be ... The Kupffer cells were activated by the presence of the hematogenous tumor cells and were able to lyse and phagocytose them. ... Highly activated Kupffer cells were observed in close contact with tumor cells in sinusoids and also in tumor forming foci ...
We found that Kupffer cells from Plasmodium-infected livers produced high levels of HGF, which trigger apoptosis of infected ... We found that Kupffer cells from Plasmodium-infected livers produced high levels of HGF, which trigger apoptosis of infected ... Using primary hepatocyte cultures, we investigated the role of Kupffer cell-derived hepatocyte growth factor (HGF) in malaria ... Our results raise the hypothesis that paracrine signals derived from Kupffer cell activation are implicated in directing death ...
Kupffer cells (KC), the resident liver macrophages, constitute the liver sinusoids together with other cells such as sinusoidal ... endothelial cells, hepatic stellate cells, liver-specific natural killer cells and dendritic cells. KC account for ... Kupffer cells are activated in cirrhotic portal hypertension and not normalised by TIPS ... approximately 10-15% of the total liver cell population and represent 80-90% of tissue macrophages in the reticuloendothelial ...
... administration is associated with suppression of Kupffer cell functioning in rat liver. Kinetics of Kupffer cell inactivation ... Administration of the Kupffer cell inactivator GdCl3 to euthyroid rats elicited a decrease in the number of ED2(+) cells, with ... Kupffer cells reside in liver sinusoids representing approximately 35% of hepatic nonparenchymal cells. These liver macrophages ... cells, a Kupffer cell subpopulation characterized with a ED2 antibody recognizing a membrane antigen of resident macrophages ...
... ,, Previous Message , Next Message ,, From:. Gayle Callis ,[email protected],. ... I need an antibody(ies) to stain canine Kupffer cells (macrophages) in liver. Have no knowledge of what crossreact to dog or a ... specific antibody for these cells. I am a mouse person, and need an answer for a bigger critter. I also would like a minirerun ...
Role of Hepatic Kupffer Cells in Inducing T cell Tolerance (128.12) Message Subject (Your Name) has forwarded a page to you ... Role of Hepatic Kupffer Cells in Inducing T cell Tolerance (128.12). Qiang You, Linling Cheng and Cynthia Ju ... The abilities of KC to activate T cells and to suppress T cell activation induced by spleen dendritic cells (DC) were examined ... Although Kupffer cells (KC) have been indicated to play a role in liver tolerance to allografts and soluble antigens, the ...
The pathogen receptor liver and lymph node sinusoidal endotelial cell C-type lectin is expressed in human Kupffer cells and ... Cun-Shuan Xu, Yun Jiang, Lian-Xing Zhang, Cui-Fang Chang, Gai-Ping Wang, Rui-Jia Shi, Yan-Jie Yang, The role of kupffer cells ... György Baffy, Kupffer cells in non-alcoholic fatty liver disease: The emerging view, Journal of Hepatology, 2009, 51, 1, 212. ... Because IL-10 is known to shorten the half-life of TNF mRNA, these results suggest that Kupffer cell production of IL-10 is an ...
Myocardial Infarction primes autoreactive T cells through activation of dendritic cells. Cell Reports. 2017. IF 7.870.. - L Van ... This project aimed to address this gap in our knowledge by generating and applying new methods to study Kupffer cells (KCs), ... Periodic Reporting for period 1 - KCs and Gut Antigens (Elucidating the role of liver resident Kupffer cells in the regulation ... CL Scott et al., Bone marrow derived monocytes give rise to self-renewing and fully differentiated Kupffer cells. Nature ...
M2 Kupffer cells promote M1 Kupffer cell apoptosis: A protective mechanism against alcoholic and nonalcoholic fatty liver ... M2 Kupffer cells promote M1 Kupffer cell apoptosis: A protective mechanism against alcoholic and nonalcoholic fatty liver ... Apoptotic cells attenuate fulminant hepatitis by priming Kupffer cells to produce interleukin-10 through membrane-bound TGF- ... Alternative M2 activation of Kupffer cells by PPARdelta ameliorates obesity-induced insulin resistance. Cell Metab 2008;7:496- ...
MRSA count was the highest in the liver, especially within Kupffer cells, which were positive for acid polysaccharides that are ... Kupffer cells; mice MRSA; virulence; biofilm; intracellular persistence; Kupffer cells; mice ... Biofilm-Forming Methicillin-Resistant Staphylococcus aureus Survive in Kupffer Cells and Exhibit High Virulence in Mice by ... MRSA count was the highest in the liver, especially within Kupffer cells, which were positive for acid polysaccharides that are ...
In addition, Kupffer cells generate increased levels of TNF-α. When Kupffer cells were inhibited, the increase in serum TNF-α ... P-selectin expression and Kupffer cell activation in rat acute pancreatitis. Autor: Folch-Puy, Emma ; Prats, Neus; Hotter, ... The generation of TNF-α by Kupffer cells was also measured. Pancreatitis increases the serum concentration of TNF-α, neutrophil ... We conclude that pulmonary inflammation induced by acute pancreatitis is mediated by Kupffer cell activation and that ...
Depletion of Liver Kupffer Cells Prevents the Development of Diet-Induced Hepatic Steatosis and Insulin Resistance. Wan Huang, ...
Benefit of Kupffer cell modulation with glycine versus Kupffer cell depletion after liver transplantation in the rat: effects ... Inhibition or destruction of Kupffer cells (KC) may protect against ischemia-reperfusion (IR) induced primary graft nonfunction ... Glycine appears to preserve cell viability and to TNFalpha/leukocyte dependent organ regeneration capacity, which is related to ... However, profound prevention of intrahepatic cell death and liver graft failure was solely achieved with glycine ...
Kupffer Cell Transplantation in Mice for Elucidating Monocyte/Macrophage Biology and for Potential in Cell or Gene Therapy.. [ ... Kupffer cells (KC) play major roles in immunity and tissue injury or repair. Because recapitulation of KC biology and function ... To promote cell engraftment through impairments in native KC, recipients were preconditioned with gadolinium chloride. The ... and function of KC within their native liver microenvironment and to develop the cell and gene therapy potential of KC ...
  • These factors regulate the phenotype of KC themselves, and the phenotypes of neighboring cells, such as hepatocytes, stellate cells, endothelial cells and other immune cells that traffic through the liver. (nih.gov)
  • The mitochondrial DNA (mtDNA) from hepatocytes of HFD-fed mice induced TNF-α and IL-6 expression in cultured Kupffer cells (KCs), which was attenuated by STING deficiency or pretreatment with BAY11-7082 (an NF-κB inhibitor). (jci.org)
  • Between the cords of hepatocytes the sinusoids were lined with endothe-lial cells having flattened dark nuclei and Kupffer cells with nuclei projecting into the sinusoids which opened into the central vein. (thefreedictionary.com)
  • Morphometric Observations: The details of Volume density of hepatocytes (Vvh) and Numerical density of Kupffer cells on the area (Nak) are summarized in Table 4. (thefreedictionary.com)
  • The researchers used vaccinia virus to infect livers that either could or could not detect type I interferon, or in which only the Kupffer cells or the hepatocytes, the main cell type of the liver, could or could not detect type I interferon. (eurekalert.org)
  • This is surprising, since hepatocytes are the main target cell for type for infection. (eurekalert.org)
  • The presence of several proliferative lesions in exposed mice, including multinucleated hepatocytes and oval and Kupffer cell hyperplasia, in the absence of cellul ar necrosis or fibrosis, indicates an isolated proliferative response, and not a regenerative response following liver injury (Thoolen et al. (thefreedictionary.com)
  • We found that Kupffer cells from Plasmodium -infected livers produced high levels of HGF, which trigger apoptosis of infected hepatocytes through a mitochondrial-independent apoptosis pathway. (frontiersin.org)
  • Our results raise the hypothesis that paracrine signals derived from Kupffer cell activation are implicated in directing death of hepatocytes infected with the malaria parasite. (frontiersin.org)
  • This response decreases liver parasite load possibly because type I IFN signaling in myeloid cells contributes to the recruitment of immune cells to the inflammatory foci around infected hepatocytes ( 6 ). (frontiersin.org)
  • T 3 -induced ROS generation occurs at different subcellular sites of hepatocytes and in the respiratory burst of Kupffer cells, triggering the activation of the transcription factors nuclear factor- κ B (NF- κ B), signal transducer and activator of transcription 3 (STAT3), and activating protein 1 (AP-1). (hindawi.com)
  • Although ICC has been defined as a tumor arising from cholangiocyte transformation, recent evidence from genetic lineage-tracing experiments has indicated that hepatocytes can be a cellular origin of ICC by directly changing their fate to that of biliary lineage cells. (sigmaaldrich.com)
  • Here, using a mouse model of ICC, we found that hepatic macrophages called Kupffer cells transiently congregate around the central veins in the liver and express the Notch ligand Jagged-1 coincident with Notch activation in pericentral hepatocytes. (sigmaaldrich.com)
  • Depletion of Kupffer cells prevents the Notch-mediated cell-fate conversion of hepatocytes to biliary lineage cells, inducing hepatocyte apoptosis and increasing mortality in mice. (sigmaaldrich.com)
  • Kupffer cells are most commonly used in co-culture systems with primary hepatocytes in drug discovery and ADME-Tox studies. (tebu-bio.com)
  • Viruses that escape these cells can then transduce hepatocytes after binding factor X (FX). (unl.edu)
  • The Kupffer cell-conditioned media were than added in separate experiments to freshly prepared isolated rat hepatocytes to determine their cytotoxic effect. (soton.ac.uk)
  • No significant effects on the rate of protein synthesis, as assessed by the incorporation of 14C-leucine on lactate dehydrogenase enzyme release from hepatocytes during 1 h incubation was found as compared with conditioned media from control Kupffer cells. (soton.ac.uk)
  • It is concluded that under the conditions of the present experiments, factors secreted by cultured Kupffer cells have no cytotoxic effects on isolated rat hepatocytes during short-term incubation. (soton.ac.uk)
  • Hepatocytes produce nitrogen oxides from L-arginine in response to inflammatory products of Kupffer cells. (rupress.org)
  • This report demonstrates that transferable products from activated Kupffer cells (KC) induce the conversion of large quantities of L-arg to nitrogen oxides within hepatocytes (HC). (rupress.org)
  • Numbers of F4/80 Kupffer cells, relative to numbers of albumin positive hepatocytes, did not show a significant trend over the first 2 postnatal weeks. (biomedcentral.com)
  • Isolated murine Kupffer cells efficiently engulfed apoptotic bodies generated from UV-treated mouse hepatocytes. (elsevier.com)
  • The increasing general agreement is that the cross-talk between hepatocytes, hepatic stellate cells (HSCs) and macrophages in NAFLD has a main role in the derangement of lipid homeostasis, insulin resistance, danger recognition, immune tolerance response and fibrogenesis. (mdpi.com)
  • They are regulated by apoptosis, as well as being phagocytized by neighbouring Kupffer cells. (wikipedia.org)
  • However, type I interferon seems to be important for Kupffer cells, says Dr. Frenz: "We believe, that type I interferon triggers Kupffer cells to take up infected cells and undergo apoptosis (suicide) afterwards, since surprisingly, Kupffer cells disappear after infection. (eurekalert.org)
  • This apoptosis mechanism is distinct from cell death that is spontaneously induced in infected cultures and is governed by Fas signaling modulation through a mitochondrial-dependent apoptosis pathway. (frontiersin.org)
  • Further investigation of the mechanism involved in such suppression revealed that KC could induce apoptosis of the activated T cells. (jimmunol.org)
  • In summary, our data suggest that KC are ¡°poor¡± stimulators of T cells, and that they can cause tolerance through induction of T cell apoptosis. (jimmunol.org)
  • Galectin-9 results in expansion of CD4+CD25+FoxP3+CD127low regulatory T cells, contraction of CD4+ effector T cells, and apoptosis of HCV-specific CTLs. (harvard.edu)
  • In vitro, PGE2 secreted by MSCs inhibited Kupffer cell apoptosis via TLR4-ERK1/2-caspase3 pathway regulation. (springer.com)
  • The results of the CCK8 assay and flow cytometry showed that, in the OGSD/R group, astrocyte cell viability was downregulated, but astrocyte apoptosis increased. (panelstaff.top)
  • It also regulates cell growth and differentiation, apoptosis, cell migration, and immune cell function in liver fibrosis/cirrhosis. (intechopen.com)
  • Hepatocyte apoptosis and of Kupffer cells (KC) and hepatic stellate cells (HSC) interaction were investigated using confocal microscopy. (biomedcentral.com)
  • In a spontaneously tolerant murine model of liver transplantation, the development of tolerance was associated with a higher rate of apoptosis of cells in the portal inflammatory infiltrate [ 5 ]. (biomedcentral.com)
  • The purpose of this study was to quantify the effects of liver transplantation on KC immunomodulatory function, focusing specifically on Fas ligand (FasL) expression and their role in allo-reactive T cell apoptosis in chronic accepted and acutely rejected hepatic allografts. (biomedcentral.com)
  • inhibition of hepatocyte apoptosis with a caspase inhibitor prevented this Kupffer cell activation. (elsevier.com)
  • LPS stimulation of KC resulted in upstream ROS generation and, subsequently, increased FasL expression and consequent Jurkat cell (Fas-positive) apoptosis. (nutriforce.cn)
  • Exogenous administration of H2O2 stimulated KC FasL expression and subsequent Jurkat cell apoptosis. (nutriforce.cn)
  • Moreover, KC can directly interact with passenger leukocytes and, thus, may play a role in immunomodulation (21), which includes antigen presentation (27) and induction of T cell apoptosis via Fas-FasL interactions (23). (nutriforce.cn)
  • abstract = "Temporal and spatial changes of lipid peroxides in a cultured colon cancer cell line, Colo-205 cells, were investigated after culturing with Kupffer cells by using 2′,7′-dichlorofluorescein diacetate and a digital imaging processor equipped with an inverted microscope. (elsevier.com)
  • abstract = "Kupffer cell function was assessed by using scintigraphy to evaluate the turnover of a metabolizable tracer (99mTc-millimicrosphered albumin). (elsevier.com)
  • Byun JS, Suh YG, Yi HS, Lee YS, Jeong WI (2013) Activation of toll-like receptor 3 attenuates alcoholic liver injury by stimulating Kupffer cells and stellate cells to produce interleukin-10 in mice. (springer.com)
  • Kupffer cells (KC), the resident liver macrophages, constitute the liver sinusoids together with other cells such as sinusoidal endothelial cells, hepatic stellate cells, liver-specific natural killer cells and dendritic cells. (bmj.com)
  • A high-cholesterol diet also induces free cholesterol accumulation in hepatic stellate cells, which are activated by inflammation, and exacerbates hepatic fibrosis induced by bile duct ligation or carbon tetrachloride treatment (12). (deepdyve.com)
  • Hepatic stellate cells and oxidative stress]. (semanticscholar.org)
  • Breiner KM, Schaller H, Knolle PA (2001) Endothelial cell-mediated uptake of a hepatitis B virus: a new concept of liver targeting of hepatotropic microorganisms. (springer.com)
  • This study has been performed to examine which cells are responsible for the hepatic clearance of the new ultrasound contrast agent Sonazoid and to study whether uptake of these gas microbubbles disturbs the function of the cells involved. (biomedsearch.com)
  • Uptake of the Sonazoid perfluorobutane microbubbles by the Kupffer cells following injection of a dose corresponding to 20x the anticipated clinical dose for liver imaging did not result in measurable changes in the uptake and degradation of radioactively labelled albumin microspheres previously shown to be a useful indicator marker for Kupffer cell phagocytosis. (biomedsearch.com)
  • Uptake and killing of Lyme disease and relapsing fever borreliae in the perfused rat liver and by isolated Kupffer cells. (prohealth.com)
  • The in vitro uptake of B. burgdorferi IRS by isolated rat Kupffer cells was rapid, and within 30 min of the infection, large intracellular aggregates of amorphous material were detectable by immunofluorescence with specific anti-B. burgdorferi antibody. (prohealth.com)
  • Rather, Ad5/6 appeared to escape Kupffer cell uptake as evidenced by producing no Kupffer cell death in vivo, not requiring predosing in vivo, and being phagocytosed less efficiently by macrophages in vitro compared to Ad5. (unl.edu)
  • In addition, the uptake of exogenous horseradish peroxidase by Kupffer cells has been investigated. (rupress.org)
  • Cells immunoreactive for F4/80 were identified as early as postnatal day 0, and these cells also displayed uptake of microspheres. (biomedcentral.com)
  • The organ uptake rate of the tracer, and new parameters concerned with the degradative functions of Kupffer cells obtained from analysis of the excretion phase of the hepatic time-uptake rate curve, were measured in rats with two different types of chronic liver damage induced by carbon tetrachloride (fatty liver group and liver cirrhosis group). (elsevier.com)
  • Tracer uptake reaches a plateau in 45 min for RAW cells and in 60 min for murine Kupffer cells. (rug.nl)
  • Hepatocyte and Kupffer Cell Interactions presents a comprehensive discussion of historical and recent information regarding this diverse field of research. (valorebooks.com)
  • In each of the book's chapters, the role of the Kupffer cell and hepatocyte interaction is placed in context with information on particular liver functions or disease states.Hepatocyte and Kupffer Cell Interactions is an essential reference for leukocyte specialists, gastroenterologists, immunologists, and other researchers working in this fascinating field.Ronald D. Curran is the author of 'Hepatocyte and Kupffer Cell Interactions', published 1992 under ISBN 9780849361098 and ISBN 0849361095. (valorebooks.com)
  • Using primary hepatocyte cultures, we investigated the role of Kupffer cell-derived hepatocyte growth factor (HGF) in malaria liver stage infection. (frontiersin.org)
  • Kupffer cells induce Notch-mediated hepatocyte conversion in a common mouse model of intrahepatic cholangiocarcinoma. (sigmaaldrich.com)
  • Notch signaling has been identified as an essential factor for hepatocyte conversion into biliary lineage cells at the onset of ICC. (sigmaaldrich.com)
  • To control the quality and reproducibility of their isolated primary Kupffer cells, Sekisui XenoTech examined 19 freshly plated hepatocyte and Kupffer cell co-cultures for the presence of Kupffer cells. (tebu-bio.com)
  • Sekisui XenoTech's Kupffer cell thawing and culturing as well as hepatocyte isolation and plating protocols assure that plated hepatocyte and Kupffer cell co-cultures contain a representative quantity of liver macrophages. (tebu-bio.com)
  • hepatocyte A specialized epithelial cell that is the most abundant type of cell in the liver . (encyclopedia.com)
  • hepatocyte (hip- at -oh-syt) n. the principal cell type in the liver: a large cell with many metabolic functions, including synthesis, storage, detoxification, and bile production. (encyclopedia.com)
  • Our results demonstrate increased hepatotoxicity and decreased survival after exposure to LPS in both of the obese strains and suggest two mechanisms-altered Kupffer cell function and increased hepatocyte sensitivity to tumor necrosis factor α (TNFα)-that might mediate obesity-related sensitivity to endotoxin. (pnas.org)
  • The role of Kupffer cells and reactive oxygen species in hepatic injury during acute and chronic alcohol intoxication. (thefreedictionary.com)
  • This contention is supported by suppression of the respiratory burst activity of Kupffer cells and the associated reactive oxygen species production by GdCl 3 or apocynin given prior to T 3 , thus hindering Nrf2 activation. (hindawi.com)
  • In addition, activated Kupffer cells produce and release several mediators including cytokines, lipid substances, and reactive oxygen species (ROS), which can function locally or systemically to mediate immune responses [ 1 ]. (hindawi.com)
  • Whereas resident Kupffer cells express F4/80 and CD68 and have high phagocytic activity and reactive oxygen species production, bone marrow-derived Kupffer cells/macrophages express F4/80 and CD11b and produce proinflammatory cytokines efficiently (8). (deepdyve.com)
  • Under these conditions, the production of reactive oxygen species (ROS) derived from activated Kupffer cells was increased, but this increase was suppressed by CO-RBC resuscitation. (elsevier.com)
  • Kupffer cells can produce inflammatory cytokines, TNF-alpha, oxygen radicals, and proteases as well as performing phagocytosis. (wikipedia.org)
  • However, inflammatory conditions allow rapid differentiation of monocytes into mature cells that are indistinguishable from genuine KC. (springer.com)
  • These functional alterations were accompanied by some histological alterations, like congestion of the central vein, some polymorphonuclear inflammatory cells infiltrate, sinusoids walls showing numerous Kupffer cells with swelling lumen by the blood cells (Figure 5). (thefreedictionary.com)
  • Cytotoxicity of Kupffer cells has been implicated in ischemia-reperfusion (IR) injury, an inflammatory model underlying drastic ROS generation [ 5 ], whereas preconditioning strategies against IR injury have been associated with moderate increases in ROS production [ 6 , 7 ]. (hindawi.com)
  • This work studied the activation of hepatic macrophages during acute pancreatitis and the involvement of these cells in the lung inflammatory response. (csic.es)
  • Liver Kupffer cells have crucial roles in mediating the inflammatory processes that promote liver cancer, but the mechanistic basis for their contributions are not fully understood. (aacrjournals.org)
  • Pro-inflammatory cytokines released by activated Kupffer cells, such as TNF-α and IL-6, are associated with up-regulation of acute-phase response proteins and suppression of CYP enzymes. (tebu-bio.com)
  • galectin-9 also induces anti-inflammatory cytokines from peripheral but not hepatic mononuclear cells. (harvard.edu)
  • Thus, dysregulation of lipid metabolism, specifically cholesterol accumulation, activates hepatic immune cells and induces hepatic inflammatory diseases including NASH. (deepdyve.com)
  • Resident macrophages (Kupffer cells, KCs) in the liver can undergo both pro- or anti-inflammatory activation pathway and exert either beneficiary or detrimental effects on liver metabolism. (biomedcentral.com)
  • The second phase is assumed to start about 8 h subsequent to CCl(4) administration and involves the oxidant-induced activation of Kupffer cells, which release various pro-inflammatory mediators such as tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6). (mysciencework.com)
  • Anichkov's cell a plump modified histiocyte in the inflammatory lesions of the heart (Aschoff bodies) characteristic of rheumatic fever. (thefreedictionary.com)
  • The overall objective of this thesis was to investigate whether hydrogen sulfide (H2S), substance P (SP) and Kupffer cells modulate the inflammatory response, organ damage and liver sinusoidal endothelial cells (LSECs) fenestrations in sepsis. (otago.ac.nz)
  • The studies described in this thesis investigated the potential roles of Kupffer cells on liver and lung injury, inflammation and the systemic inflammatory response in sepsis using gadolinium chloride (GdCl3) to inactivate these cells. (otago.ac.nz)
  • Generally, liver fibrosis begins with the stimulation of inflammatory immune cells to secrete cytokines, growth factors, and other activator molecules. (intechopen.com)
  • Hepatic CYP protection after a hemorrhage and CO-RBC resuscitation can be attributed to the inactivation of Kupffer cells, resulting in the suppression of ROS production in the early phase and the suppression of inflammatory cytokine production via the TLR-4/HMGB-1signal pathway in the late phase. (elsevier.com)
  • However, in some cell types it is detectable only when up-regulated, such as activated but not quiescent microglia, and can thus be used as a marker of inflammatory conditions and immune reactions in those instances. (wikipedia.org)
  • Kupffer cells ( kuup -fer) pl. n. phagocytic cells that line the sinusoids of the liver (see macrophage ). (encyclopedia.com)
  • However, the role of stimulating macrophage colony seems to be the most important in the developing of mature Kupffer cells. (wikipedia.org)
  • Fixed MACROPHAGE cells that line the fine blood sinuses (capillaries) of the liver and act as scavengers to remove senescent red blood cells, bacteria and other foreign material. (thefreedictionary.com)
  • Through this fellowship, we have greatly enhanced our understanding of KC biology and more generally macrophage and dendritic cell (DC) biology, the key cell types, present in almost all organs, which acquire and cue the immune system to respond appropriately to foreign and host agents. (europa.eu)
  • Cells were evaluated for expression of CD68, a general macrophage marker, by immunofluorescent staining after 3 days in culture. (tebu-bio.com)
  • Because ER stress is known to induce M2 macrophage polarization, we examined whether Nogo-B regulates M1/M2 polarization of Kupffer cells and alters the pathogenesis of alcoholic liver disease (ALD). (elsevier.com)
  • Kupffer cells (KC), the resident macrophage population in the liver, are found within the sinusoidal lumen, adhering to the liver sinusoidal endothelial cells (LSEC). (biomedcentral.com)
  • The cells showed typical macrophage morphologic features and stained positively for nonspecific esterase and both ED1 and ED2, and phagocytosed 1 micrometer Latex beads. (biomedcentral.com)
  • Kupffer cells of the developing mouse liver appear quite similar to those of other mammalian species, confirming that the mouse presents a useful animal model for studies of liver macrophage developmental structure and function. (biomedcentral.com)
  • Our results showed that AS increased FITC-LPS internalization by peritoneal macrophage and liver Kupffer cell, but enhancement of LPS internalization by AS was not related to the clathrin-dependent pathway. (mdpi.com)
  • Kupffer cells (KC), the resident macrophage population in the liver, comprise one of major populations (20%) of the hepatic nonparenchymal cells. (nutriforce.cn)
  • It is particularly useful as a marker for the various cells of the macrophage lineage, including monocytes, histiocytes, giant cells, Kupffer cells, and osteoclasts. (wikipedia.org)
  • Periportal Kupffer cells tend to be larger and have more lysosomal enzyme and phagocytic activity, whereas centrilobular Kupffer cells create more superoxide radical. (wikipedia.org)
  • Electron microscopy showed latex in Kupffer cells, more abundantly seen in high-parasitemic animals.Total Kupffer cell phagocytic activity of the liver is markedly increased in rats with a high parasitemic load of malarial P. berghei infection. (austin.org.au)
  • The data demonstrated a reduced phagocytic activity of the Kupffer cells in rats with chronic liver injury. (elsevier.com)
  • The data showed that the impairment of Kupffer cell degradative function occurred even earlier in liver damage than impairment of the phagocytic activity, so that the K value and the D 60 value were the more sensitive indicators of Kupffer cell function. (elsevier.com)
  • In isolated Kupffer cells from rats treated with ethanol for 4 weeks, CD14, LPS-induced intracellular Ca2+, and TNF-alpha all were increased. (nih.gov)
  • MRSA count was the highest in the liver, especially within Kupffer cells, which were positive for acid polysaccharides that are associated with intracellular biofilm. (mdpi.com)
  • The present study suggested that the resident Kupffer cell-derived oxidative stress participates in the cytotoxic process against cancer cells by inducing intracellular lipid peroxidation. (elsevier.com)
  • We non-invasively monitored how this altered the motion of intracellular phagosomes in Kupffer cells using magnetometry. (core.ac.uk)
  • Agents that elevated intracellular cAMP levels (e.g. forskolin, dibutyryl cAMP, cholera toxin, and isoproterenol) markedly decreased nitrite production and iNOS protein formation by LPS-stimulated Kupffer cells. (uthscsa.edu)
  • Taken together, this study indicates that the attenuation of LPS- induced iNOS formation in Kupffer cells by elevated intracellular cAMP levels occurs by preventing the degradation of IκBα which suppresses the activation of NF-κB and inhibits the onset of transcription of the iNOS gene. (uthscsa.edu)
  • Cannabinoid CB2 receptors protect against alcoholic liver disease by regulating Kupffer cell polarization in mice. (thefreedictionary.com)
  • An electron microscopy study of Kupffer cells in livers of mice having Friend erythroleukemia hepatic metastases. (nih.gov)
  • We have also developed precise methods to identify and isolate different immune cells which respond to such antigens from mice and men, a process crucial to our better understanding of the specific functions of each of these cell types. (europa.eu)
  • Oyama T, Miyazaki M, Yoshimura M, Takata T, Ohjimi H, Jimi S. Biofilm-Forming Methicillin-Resistant Staphylococcus aureus Survive in Kupffer Cells and Exhibit High Virulence in Mice. (mdpi.com)
  • Adoptive transfer of wild-type Kupffer cells to Trem1 -deficient mice complemented these defects and reversed unresponsiveness to DEN-induced liver injury and malignant development. (aacrjournals.org)
  • In this study, we investigated the role of LXRα in the pathogenesis of steatohepatitis by assessing the effects of a high-fat and high-cholesterol diet (HFCD) on hepatic immune cell proportion and function as well as lipid metabolism in wild-type (WT) and LXRα-KO mice. (deepdyve.com)
  • These mice had higher cholesterol levels in the plasma and the liver and dysregulated expression of LXR target and proinflammatory genes in both whole liver samples and isolated hepatic mononuclear cells. (deepdyve.com)
  • Flow cytometry showed an increase in CD68+CD11b+ Kupffer cells/macrophages and a decrease in invariant natural killer T cells in the liver of HFCD-fed LXRα-KO mice. (deepdyve.com)
  • Mice fed a high-cholesterol diet or a high-fat and high-cholesterol diet (HFCD) have increased numbers of CD11b+ Kupffer cells/macrophages in the liver and are highly susceptible to acute hepatic inflammation induced by cytosine-phosphate-guanine oligonucleotide or α-galactosylceramide (α-GalCer) administration (10, 11). (deepdyve.com)
  • Decreasing CB1 receptor signaling in Kupffer cells improves insulin sensitivity in obese mice. (dbcls.jp)
  • We investigated the role of Kupffer cells during CCl(4) intoxication using Nucling-knockout mice (the KO group), in which the number of Kupffer cells is largely reduced. (mysciencework.com)
  • Kupffer cells isolated from WT and Nogo-B KO mice were assessed for M1 and M2 activation. (elsevier.com)
  • Conclusion: Nogo-B is permissive of M1 polarization of Kupffer cells, thereby accentuating liver injury in ALD in humans and mice. (elsevier.com)
  • Kupffer cells are well known macrophages of the liver, however, the developmental characteristics of Kupffer cells in mice are not well understood. (biomedcentral.com)
  • Although several studies have examined varied aspects of Kupffer cell function in mice, there has not been, to our knowledge, a study of the basic characteristics and the postnatal development of Kupffer cells in mice. (biomedcentral.com)
  • Because of the important role that will be played by mice in future studies of liver function, it is imperative to establish the baseline of normal Kupffer cell composition to serve as a reference for these future studies. (biomedcentral.com)
  • The purpose of this study was to identify and characterize Kupffer cells in the livers of postnatal mice, and to determine the age in mice at which Kupffer cells are phagocytically active. (biomedcentral.com)
  • After baseline measurements of relaxation, the mice were injected intravenously with Plasmodium chabaudi-parasitized or normal murine red blood cells (RBCs). (core.ac.uk)
  • Bieghs V, Walenbergh SM, Hendrikx T, van Gorp PJ, Verheyen F, Olde Damink SW et al (2013) Trapping of oxidized LDL in lysosomes of Kupffer cells is a trigger for hepatic inflammation. (springer.com)
  • Targeting of markers expressed on KCs and inflamed epithelium offers perspectives for monitoring the inflammation development and understanding the type of specific cells and receptors involved ( 7 ). (frontiersin.org)
  • On the other hand, type I interferons delay the regeneration of immune cells, which are important to adjust and maintain the immune balance within the liver during acute inflammation. (eurekalert.org)
  • Some of the highlights include the concept of Kupffer cell activation and the role of these cells in liver inflammation, oxidative stress--related injury, stellate cell activation, and liver fibrosis. (thefreedictionary.com)
  • We conclude that pulmonary inflammation induced by acute pancreatitis is mediated by Kupffer cell activation and that pancreatitis induces the expression of P-selectin on pulmonary endothelial cells but this effect is not mediated by Kupffer cells. (csic.es)
  • Together, our findings offer causal evidence that TREM-1 is a pivotal determinant of Kupffer cell activation in liver carcinogenesis, deepening mechanistic insights into how chronic inflammation underpins the development and progression of liver cancer. (aacrjournals.org)
  • Kupffer primary cells are macrophages endogenous to the liver which have the ability to modulate hepatic inflammation and injury associated with various pathophysiologies and toxicities. (tebu-bio.com)
  • A number of immune cells, including Kupffer cells, natural killer (NK) cells, and natural killer T (NKT) cells, are present in the liver and participate in the induction and progression of hepatic inflammation (7). (deepdyve.com)
  • Kupffer cells are tissue-resident macrophages in the liver that play an important role in inflammation associated with infection. (otago.ac.nz)
  • LSECs are specialised fenestrated endothelial cells in the liver that undergo structural alteration during inflammation and infection. (otago.ac.nz)
  • Although CXCL10 is also known to participate in the pathogenesis of hepatic inflammation , the degree to which it is functionally involved in the crosstalk between immune cells and regulation of immune response is still unclear. (bvsalud.org)
  • Our aim was to ascertain whether engulfment of apoptotic bodies by Kupffer cells promotes hepatic inflammation and fibrosis. (elsevier.com)
  • Consistent with a role for Kupffer cells in liver inflammation and fibrosis, gadolinium chloride attenuated neutrophil infiltration and markers for stellate cell activation. (elsevier.com)
  • In conclusion, these findings support a model of cholestatic liver injury where Kupffer cell engulfment of apoptotic bodies promotes inflammation and fibrogenesis. (elsevier.com)
  • We propose that cFn directly stimulates Kupffer cells (KCs), which are involved in the early response to tissue damage, to produce factors that enhance the progression of alcohol-induced liver injury toward inflammation and fibrosis. (nebraska.edu)
  • 4]administration oxidant induced activation of kupffer cells occur, with the release of proinflammatory cytokines like tumor TNF-[alpha], TGF-[beta] and IL-6. (thefreedictionary.com)
  • At steady state , elimination of KCs results in a reduction of hepatic infiltrating monocytes , T, B, and NK cells and a list of cytokines and chemokines at transcriptional level. (bvsalud.org)
  • In the pathological condition, the KCs elimination rescues Con A-induced acute hepatitis through suppressing proinflammatory immune responses by down-regulation of hepatitis -associated cytokines / chemokines in serum such as CXCL10, and recruitment of infiltrating immune cells ( monocytes , T, B, and NK cells ). (bvsalud.org)
  • The effect of exogenous cFn on KC viability and the secretion of the cytokines, TNF-α and IL-6, as well as of matrix remodeling factors, MMP-2 and TIMP-2, was determined after 20hours of cell culture. (nebraska.edu)
  • These changes were suppressed by GdCl 3 treatment prior to T 3 , an agent-eliciting Kupffer-cell depletion, inhibition of colloidal carbon phagocytosis, and the associated respiratory burst activity, with enhancement in nuclear inhibitor of Nrf2 kelch-like ECH-associated protein 1 (Keap1)/Nrf2 content ratios suggesting Nrf2 degradation. (hindawi.com)
  • Benefit of Kupffer cell modulation with glycine versus Kupffer cell depletion after liver transplantation in the rat: effects on postischemic reperfusion injury, apoptotic cell death graft regeneration and survival. (sigmaaldrich.com)
  • In conclusion, interruption of TNF-alpha signaling or depletion of Kupffer cells improves survival after 30% liver transplantation, reduces liver injury, and enhances regeneration. (uzh.ch)
  • It is possible for the Kupffer cells to take in large particles by phagocytosis and smaller particles via pinocytosis. (wikipedia.org)
  • Glauhann H. (1976) Phagocytosis and Intralysosomal Digestion of Subcellular Organelles by Kupffer Cells of Rat Liver. (springer.com)
  • Both apoptotic body phagocytosis and death ligand generation were attenuated by gadolinium chloride, a Kupffer cell toxicant. (elsevier.com)
  • Monocytes that are in the peripheral circulation and come from precursor cells in the bone marrow, are thought to be immature precursors for tissue macrophages, in adult animals. (wikipedia.org)
  • The presence of F4/80 antigen (which becomes expressed as monocytes mature into tissue macrophages) on sinusoidal liver cells has been used to "define" KCs ( 9 ). (frontiersin.org)
  • Bone marrow derived monocytes give rise to self-renewing and fully differentiated Kupffer cells. (europa.eu)
  • This suggests that CLEC4F is a C-type lectin with diverse binding specificity expressed on residential Kupffer cells and infiltrating monocytes in the liver, and may play an important role to modulate glycolipids presentation on Kupffer cells. (nih.gov)
  • CXCL10 is a chemokine that has been implicated in chemoattraction of monocytes , T cells , NK cells , and dendritic cells , and promotion of T cell adhesion to endothelial cells . (bvsalud.org)
  • monocytes/macrophages are the primary cells of the innate immune system. (mdpi.com)
  • Effect of quinine on chemiluminescence response of Kupffer cells, blood monocytes & peritoneal macrophages of normal guineapigs. (bvsalud.org)
  • ED1 is the most widely used monoclonal antibody clone directed against the rat CD68 protein and is used to identify macrophages, Kupffer cells, osteoclasts, monocytes, and activated microglia in rat tissues. (wikipedia.org)
  • It was concluded that pretranscriptional regulation of TNF gene expression in Kupffer cells follows an orderly activation of p38-MAPK, ERK1/2, and SAPK/JNK that may not converge on NF-kappaB. (unboundmedicine.com)
  • This study tested a hypothesis that iron directly elicits the signaling required for activation of NF-κB and stimulation of tumor necrosis factor (TNF)-α gene expression in Kupffer cells. (elsevier.com)
  • The amoeboid shaped Kupffer cells are attached to sinusoidal endothelial cells. (wikipedia.org)
  • Physically, Kupffer cells (KCs) are interspersed with fenestrated liver sinusoidal endothelial cells in a mosaic fashion to make up the sinusoidal lining. (frontiersin.org)
  • Compared with the normal liver tissues from the control group, in knockouts following LPS treatment significant evidence of injury with severe sinusoidal vascular congestion, mild neutrophilic infiltration and Kupffer cell activation were demonstrated by some previous researchers (Konyalioglu et al. (thefreedictionary.com)
  • In 25-40% of sinusoidal cells, an electron-opaque reaction product is localized in segments of the endoplasmic reticulum, including the perinuclear cisternae, a few Golgi vesicles and saccules and in some large membrane-bounded granules. (rupress.org)
  • Kupffer cells promote T-cell hepatitis by producing CXCL10 and limiting liver sinusoidal endothelial cell permeability. (bvsalud.org)
  • Rationale Kupffer cells (KCs) play a crucial role in liver immune homeostasis through interacting with other immune cells and liver sinusoidal endothelial cells (LSECs). (bvsalud.org)
  • Lipopolysaccharide (LPS) is a bacterial endotoxin which is found in the cell wall gram-negative bacteria, whereas lipoteichoic acid is present in gram-positive bacteria. (wikipedia.org)
  • Research funded by the National Institute on Alcohol Abuse and Alcoholism (NIAAA)/National Institutes of Health (NIH) first identified the importance of Kupffer cells and their response to endotoxin in the progression of liver disease (Thurman et al. (thefreedictionary.com)
  • Thus, it was hypothesized that Kupffer cell activation by endotoxin derived from intestinal bacteria causes those cells to generate superoxide and TNF-[alpha], both of which can lead to tissue damage in the liver (see figure 1). (thefreedictionary.com)
  • These findings confirm that the binding of endotoxin to its receptors on the Kupffer cells and the resulting Kupffer cell activation and production of TNF-[alpha] are key events in the development of alcoholic liver injury. (thefreedictionary.com)
  • Endotoxin binds to Kupffer cell receptors, initiating a cascade of events that lead to the release of a chemical messenger (i. (thefreedictionary.com)
  • Studies with gut sterilization and Kupffer cell inactivation support the hypothesis that endotoxin and Kupffer cells are involved in mechanisms of alcohol-induced liver injury. (nih.gov)
  • Recently, we found that Kupffer cells isolated from rats treated only once with ethanol were sensitized to endotoxin 24 hr later. (nih.gov)
  • The cytokine response of isolated Kupffer cells to stimulation with bacterial endotoxin (LPS) in vitro has been characterized by Sekisui XenoTech's scientists (Lambert et al. (tebu-bio.com)
  • Kupffer cells (KCs), phagocytic liver-resident macrophages, provide a protective barrier against egress of endotoxin from the portal to the systemic circulation. (bmj.com)
  • Activated Kupffer cells may release substances that are involved in liver injury induced by galactosamine and endotoxin. (soton.ac.uk)
  • In the present study Kupffer cells were isolated from rat livers, cultured for 24 hours and incubated with galactosamine, endotoxin (LPS) or tumor necrosis factor-alpha for 4 or 24 hours. (soton.ac.uk)
  • Barsig J, Flesch IE, Kaufmann SH (1998) Macrophages and hepatocytic cells as chemokine producers in murine listeriosis. (springer.com)
  • Mechanism of T cell tolerance induction by murine hepatic Kupffer cells. (semanticscholar.org)
  • The purpose of this study was to determine the mechanisms by which alcohol sensitizes Kupffer cells to lipopolysaccharide (LPS). (nih.gov)
  • The purpose of this study was to examine whether puerarin ameliorates chronic alcoholic liver injury through inhibition of endotxin gut-leakage, the subsequent Kupffer cells (KCs) activation and lipopolysaccharide (LPS) receptors expression. (aspetjournals.org)
  • Suppression of lipopolysaccharide-stimulated release of tumor necrosis factor by adenosine: evidence for A2 receptors on rat Kupffer cells. (duke.edu)
  • To test the hypothesis that these agents act by suppressing cytokine release by activated Kupffer cells, we assessed the effect of each drug on tumor necrosis factor released from cultured rat Kupffer cells stimulated with lipopolysaccharide. (duke.edu)
  • Treatment of cultured rat Kupffer cells with lipopolysaccharide (LPS) resulted in a time-dependent increase in the expression of the inducible isoform of nitric-oxide synthase (iNOS). (uthscsa.edu)
  • Canbay A, Feldstein AE, Higuchi H, Werneburg N, Grambihler A, Bronk SF et al (2003) Kupffer cell engulfment of apoptotic bodies stimulates death ligand and cytokine expression. (springer.com)
  • Inhibition or destruction of Kupffer cells (KC) may protect against ischemia-reperfusion (IR) induced primary graft nonfunction (PNF) in liver transplantation. (sigmaaldrich.com)
  • The Biological Function of Kupffer Cells in Liver Disease, Biology of Myelomonocytic Cells Anirban Ghosh, IntechOpen, DOI: 10.5772/67673. (intechopen.com)
  • Histologic evidence suggests two possibilities: a localized impairment of Kupffer cell activity in the area about the central veins of the lobule, or an abnormal circulation through preferential pathways restricted to the periphery of the liver lobule. (ahajournals.org)
  • They were cleared from the blood, primarily by Kupffer cells, and retained within their phagosomes. (core.ac.uk)
  • In earlier work (5,6,8) an experimental model was used in which cellular organelles were introduced to the lysosomal apparatus of Kupffer cells by means of heterophagocytosis. (springer.com)
  • These results indicated that excess reactions of Kupffer cells, once primed by oxidants, were involved in the exacerbation of oxidative stress and liver damage during the second phase of CCl(4) intoxication. (mysciencework.com)
  • Moreover, several evidences have suggested that hepatic stem/progenitor cell (HPCs) activation is a component of the adaptive response of the liver to oxidative stress in NAFLD. (mdpi.com)
  • A significant positive correlation was observed between Nogo-B positive Kupffer cells and disease severity in ALD patients (n = 30, r = 0.66, P = 0.048). (elsevier.com)
  • Furthermore, Nogo-B-positive Kupffer cells were correlated with M1 activation (inducible nitric oxide synthase) (r = 0.50, P = 0.05) and negatively with markers of M2 status (CD163) (r = −0.48, P = 0.07) in these patients. (elsevier.com)
  • The F4/80 positive Kupffer cells were labelled with both large (0.2 μm) and small (0.02 μm) diameter microspheres, while endothelial cells were labelled only with the smaller diameter microspheres. (biomedcentral.com)
  • 4] for a similar period showed dose- and duration-dependant protective effects, such as diminution of fibrosis and fatty changes, while dilated blood sinusoids, vacuolar degeneration and hypertrophy of Kupffer cells were also seen, when compared to the liver sections of rats intoxicated with [CCl. (thefreedictionary.com)
  • Activation of in vivo Kupffer cell function by oral administration of Cordyceps sinensis in rats. (thefreedictionary.com)
  • Kupffer cells isolated from rats treated with ethanol for 4 weeks exhibit sensitization to LPS. (nih.gov)
  • Sonazoid was injected into rats and perfused fixed livers were studied by electron microscopy, which revealed that the Sonazoid microbubbles were exclusively internalised in Kupffer cells, i.e. by the macrophages located in the liver sinusoids, and not by parenchymal, stellate or endothelial cells. (biomedsearch.com)
  • Role of Kupffer cells in reperfusion injury in fat-loaded livers from ethanol-treated rats. (aspetjournals.org)
  • Pretreatment of rats with gadolinium chloride (GdCl3, 20 mg/kg i.v.), a selective Kupffer cell toxicant, minimized lactate dehydrogenase release during reperfusion to 7 to 8 IU/g/h in livers from both groups. (aspetjournals.org)
  • Inactivation of Kupffer cells minimized reperfusion injury in both control and alcohol-treated rats, most likely by diminishing lipid peroxidation thereby improving hepatic microcirculation. (aspetjournals.org)
  • We have investigated the effect of Kupffer cell blockade GdCl3 on liver function in warm ischemic model in rats. (nii.ac.jp)
  • Austin Health Research Online: Hepatic Kupffer cell phagocytotic function in rats with erythrocytic-stage malaria. (austin.org.au)
  • Hepatic Kupffer cell phagocytotic function in rats with erythrocytic-stage malaria. (austin.org.au)
  • Methods: Sprague-Dawley rats were divided into two groups: one group received a single dose of GdCl (a Kupffer cell-blocking agent, 10 mg/kg i.v.), whereas the other group received saline. (edu.au)
  • Thereafter the pharmacological properties of this radiotracer were explored in a range of pre-clinical models including cells and wistar-rats. (rug.nl)
  • METHODS Kupffer cells isolated from Sprague-Dawley rats were cultured in the presence of LPS added alone or with SST for 12,24 and 48 h,and the production of TNF? (bvsalud.org)
  • The SR-AI/II scavenger receptor is found inside Kupffer cells. (wikipedia.org)
  • In this study, we developed a series of nanobodies by a phage display screening building from lymphocytes isolated from an alpaca immunized with recombinant mouse Kupffer cell receptor Clec4F, which is involved in pathogen recognition by binding to galactose and N-acetylgalactosamine. (frontiersin.org)
  • Taken together, we developed a series of nanobodies targeting multiple distinct recognition epitopes of the Kupffer cell-specific receptor Clec4F which may be useful for its structural and functional investigation as well as for use as molecular imaging and therapeutic agents. (frontiersin.org)
  • KCs express several cell-surface receptors and receptor complexes involved in immune stimulation ( 5 ). (frontiersin.org)
  • In fact, recruitment of natural killer T (NKT) cells in sporozoites-infected livers was shown to be dependent on the expression of interferon-alpha/beta receptor alpha chain ( 5 ). (frontiersin.org)
  • Here we show that expression of the proinflammatory myeloid cell surface receptor TREM-1 expressed by Kupffer cells is a crucial factor in the development and progression of liver cancer. (aacrjournals.org)
  • Role of Kupffer cells and toll-like receptor 4 in acetaminophen-induced acute liver failure. (harvard.edu)
  • The LT-β ligand and receptor were expressed by isolated normal Kupffer cells. (edu.au)
  • This method allows quantitative and non-invasive imaging of liver function by using expression of the Kupffer cell specific CD206 receptor with special interest in liver toxicity and the early events leading to liver fibrosis. (rug.nl)
  • This project aimed to address this gap in our knowledge by generating and applying new methods to study Kupffer cells (KCs), which are not only the most abundant immune cells in the liver but also the first immune cells to be in contact with materials entering the liver from the intestine as they reside in the blood flow in the liver sinusoids. (europa.eu)
  • Here, we hypothesize that the removal of the liver macrophages, cells that have been reported to take up the largest amount of circulating nanoparticles, would lead to a significant increase in the nanoparticle delivery efficiency to solid tumors. (nih.gov)
  • cytokine) called tumor necrosis factor alpha (TNF-[alpha]) from Kupffer cells (Nakamura et al. (thefreedictionary.com)
  • XenoTech offers Kupffer cells to aid in the assessment of cytokine release by biologics and small molecule drugs. (tebu-bio.com)
  • Kupffer cells, named after Karl Wilhelm von Kupffer (1829-1902), are found in the bloodstream and in the liver, attached to the walls of the sinusoids . (encyclopedia.com)
  • Kupffer cells, also known as stellate macrophages and Kupffer-Browicz cells, are specialized cells localized in liver within the lumen of the liver sinusoids and are adhesive to their endothelial cells which make up the blood vessel walls. (wikipedia.org)
  • Highly activated Kupffer cells were observed in close contact with tumor cells in sinusoids and also in tumor forming foci within the hepatic parenchyma. (nih.gov)
  • Kupffer cells and other macrophages were found to leave the sinusoids and migrate to sites of potential tumor development where they interacted with tumor cells and intimately wrapped their processes around fat storing cells. (nih.gov)
  • Kupffer cells reside in liver sinusoids representing approximately 35% of hepatic nonparenchymal cells. (hindawi.com)
  • Kupffer cells are specialized macrophages lining the walls of the sinusoids in the liver. (cellapplications.com)
  • Among the different cells in hepatic sinusoids, the nonphagocytic "fat-storing" cells (39) are always peroxidase negative, whereas the lining cells in process of erythrophagocytosis are consistently peroxidase positive. (rupress.org)
  • In ex vivo study using isolated perfused rat liver, dichlorofluorescin-preloaded cancer cells, which were transportally injected, were found to adhere to hepatic sinusoids and then to enhance their fluorescence. (elsevier.com)
  • Kupffer cells form a partial lining of the liver sinusoids, acting to phagocytose foreign particulate matter from the circulating blood. (biomedcentral.com)
  • There they complete their differentiation into Kupffer cells. (wikipedia.org)
  • BioTime develops and markets research products in the field of stem cells and regenerative medicine, including a wide array of proprietary ACTCellerate™ cell lines, culture media, and differentiation kits. (fiercebiotech.com)
  • CD68 (Cluster of Differentiation 68) is a protein highly expressed by cells in the monocyte lineage (e.g., monocytic phagocytes, osteoclasts), by circulating macrophages, and by tissue macrophages (e.g. (wikipedia.org)
  • We have demonstrated that Kupffer cell-derived tumor necrosis factor (TNF) mediates pancreatitis-associated liver injury. (unboundmedicine.com)
  • Overproduction of tumor necrosis factor by activated Kupffer cells may cause primary graft nonfunction, multiple organ failure and, eventually, death of graft recipients. (duke.edu)
  • Engulfment of the apoptotic bodies, but not latex beads, stimulated Kupffer cell generation of death ligands, including Fas ligand, and tumor necrosis factor α (TNF-α). (elsevier.com)
  • The role of Kupffer cells and hepatoctyes in normal physiology, nonseptic pathological states, and in sepsis is examined. (valorebooks.com)
  • Kupffer cells, which are part of the reticuloendothelial system, play an important role in clearing pathogenic substances, including tumor cells, from the liver. (nih.gov)
  • The role of Kupffer cells in tumor development is very important as Kupffer cells can be manipulated to a tumoricidal state with biological response modifiers to kill tumor cells and thus to decrease tumor burden and extend survival time. (nih.gov)
  • To gain additional information on the role of Kupffer cells and their interaction with tumor cells in hepatic metastases, we studied an established experimental hematogenous metastatic model (Friend erythroleukemia) in mouse livers by light and electron microscopy. (nih.gov)
  • Although Kupffer cells (KC) have been indicated to play a role in liver tolerance to allografts and soluble antigens, the mechanisms involved remain unclear. (jimmunol.org)
  • The aim of this study was to determine the role of p38 mitogen-activated protein kinase (MAPK), extracellular stress-related kinase 1/2 (ERK1/2), stress-activated protein kinase/c-Jun N-terminal kinase (SAPK/JNK), and nuclear factor-kappaB (NF-kappaB) in TNF gene expression within Kupffer cells. (unboundmedicine.com)
  • Mesenchymal stem cells (MSCs) have been proposed as a promising therapy for treatment of certain liver diseases, but the role of MSCs in DCD liver graft function remains elusive. (springer.com)
  • These findings clearly indicate that Kupffer cell activation plays an important role in cold and warm ischemic insult in rat liver. (nii.ac.jp)
  • Role of Kupffer cells in the induction of tolerance after liver transplantation. (semanticscholar.org)
  • The role of Kupffer cells (KC) in the induction of tolerance caused by preoperative donor-specific blood transfusion (DST) was investigated. (semanticscholar.org)
  • The role of Kupffer cells in carbon tetrachloride intoxication. (mysciencework.com)
  • It may be sustained that Kupffer cells play a role in the host defence mechanisms against the liver metastasis of colon cancer cells. (elsevier.com)
  • The role of Kupffer cells (KCs) in the pathophysiology of the liver has been firmly established. (ovid.com)
  • CXCL10 neutralizing antibody and CXCL10 deficient mouse were used to study the role of CXCL10 in immune cell migration and pathogenesis of Con A-induced hepatitis . (bvsalud.org)
  • KC, which comprise one of major populations (20%) of the hepatic nonparenchymal cell fraction (NPC), can directly interact with passenger leukocytes and thus may play a role in immunomodulation and the induction of tolerance [ 7 ]. (biomedcentral.com)
  • Although the Fas-FasL system has been widely studied for its role in tissue growth (1) and elimination of malignant (16) and damaged cells (10), its role is perhaps best characterized in the immune system. (nutriforce.cn)
  • Background and Aims: Kupffer cells are responsible for maintaining liver homeostasis and have a vital role in chronic hepatotoxicity and various liver diseases. (rug.nl)
  • Gaudio, E. Role of Hepatic Progenitor Cells in Nonalcoholic Fatty Liver Disease Development: Cellular Cross-Talks and Molecular Networks. (mdpi.com)
  • ET-3-induced metabolism of phosphoinositides was inhibited completely in Kupffer cells pretreated with ET-3, suggesting homologous ligand-induced desensitization of the ET-3 receptors. (biochemj.org)
  • Treatment of Kupffer cells with LPS induced the translocation of the p65 subunit of nuclear factor κB (NF-κB) into the nucleus, and this process was abolished by forskolin. (uthscsa.edu)
  • C) Coexpression of CLEC4F and F4/80 on Kupffer cells, but not peritoneal macrophages. (nih.gov)
  • The significance of these observations in respect to some recent studies suggesting localization of exogenous peroxidases in the endoplasmic reticulum of Kupffer cells and peritoneal macrophages (22, 23) is briefly discussed. (rupress.org)
  • The abilities of KC to activate T cells and to suppress T cell activation induced by spleen dendritic cells (DC) were examined by in vitro proliferation assays using OTII cells. (jimmunol.org)
  • Neoplastic and in vitro cultured cells apart, endothelium cialis over the counter at walmart reviews is the only nonmyofibroblastic cell type to show well-developed fibronexus junctions. (panelstaff.top)
  • The inclusion of Kupffer cells in in vitro hepatic cultures provides researchers with advanced tools for disease modeling and drug discovery. (fiercebiotech.com)
  • Mustafa, SB & Olson, MS 1998, ' Expression of nitric-oxide synthase in rat Kupffer cells is regulated by cAMP ', Journal of Biological Chemistry , vol. 273, no. 9, pp. 5073-5080. (uthscsa.edu)
  • Here, we demonstrate that galectin-9, the natural ligand for the T cell immunoglobulin domain and mucin domain protein 3 (Tim-3), circulates at very high levels in the serum and its hepatic expression (particularly on Kupffer cells) is significantly increased in patients with chronic HCV as compared to normal controls. (harvard.edu)
  • Removal of a portion of Kupffer cells ensure host immunity is not significantly suppressed and provides improved pharmacokinetics of gold nanoparticles by preventing off-target accumulation in the liver. (nih.gov)
  • Kupffer cells (KC) constitute 80-90% of the tissue macrophages present in the body. (nih.gov)
  • KC account for approximately 10-15% of the total liver cell population and represent 80-90% of tissue macrophages in the reticuloendothelial system. (bmj.com)
  • It is because of this that any change to Kupffer cell functions can be connected to various liver diseases such as alcoholic liver disease, viral hepatitis, intrahepatic cholestasis, steatohepatitis, activation or rejection of the liver during liver transplantation and liver fibrosis. (wikipedia.org)
  • Another line of evidence indicates that activation of Kupffer cells is a key component in the outcome of malaria liver stage infection. (frontiersin.org)
  • We hypothesized that KC could induce T cell tolerance by acting as ¡°poor¡± antigen presenting cells, as well as an active suppressor of T cell activation. (jimmunol.org)
  • Myocardial Infarction primes autoreactive T cells through activation of dendritic cells. (europa.eu)
  • Besides KC activation, PNF is characterized by microvascular perfusion failure, intrahepatic leukocyte accumulation, cell death and hepatocellular dysfunction. (sigmaaldrich.com)
  • TNF, TNF-mRNA, NF-kappaB activation, and phosphorylated p38-MAPK, SAPK/JNK, and ERK1/2 were determined in Kupffer cells treated with elastase. (unboundmedicine.com)
  • Trem1 deficiency attenuated Kupffer cell activation by downregulating transcription and protein expression of interleukin (IL)-6, IL-1β, TNF, CCL2, and CXCL10. (aacrjournals.org)
  • In addition, Trem1 ablation diminished activation of the p38, extracellular regulated kinase 1/2, JNK, mitogen-activated protein kinase, and NF-κB signaling pathways in Kupffer cells, resulting in diminished liver injury after DEN exposure. (aacrjournals.org)
  • For new biological entities, particularly immunomodulators, evaluating the potential for Kupffer cell activation is an emerging concept in preclinical development. (tebu-bio.com)
  • The findings provide evidence for activation of bone marrow-derived Kupffer cells/macrophages and dysfunction of invariant natural killer T cells in LXRα-KO mouse liver. (deepdyve.com)
  • Curcumin reduces Ly6Chi monocyte infiltration to protect against liver fibrosis by inhibiting Kupffer cells activation to reduce chemokines secretion. (dbcls.jp)
  • Addition of Fe 2- to the cells resulted in an increase in electron paramagnetic resonance-detectable ·OH peaking at 15 min, preceding activation of NF-κB but coinciding with activation of inhibitor κB kinase (IKK) but not c-Jun NH 2 -terminal kinase. (elsevier.com)
  • Without any prior activation, Kupffer cells isolated from an intact rat liver caused rapid increase in the intensity of dichlorofluorescein in tumor cells in a time-dependent manner. (elsevier.com)
  • However, profound prevention of intrahepatic cell death and liver graft failure was solely achieved with glycine preconditioning. (sigmaaldrich.com)
  • Glycine appears to preserve cell viability and to TNFalpha/leukocyte dependent organ regeneration capacity, which is related to increase graft survival following liver transplantation. (sigmaaldrich.com)
  • Gut bacteria, bacterial endotoxins, and microbial debris transported to the liver from the gastrointestinal tract via the portal vein will first come in contact with Kupffer cells, the first immune cells in the liver. (wikipedia.org)
  • The identification of tissue-resident macrophages and their unique feature in relation to other immune cells has so far mainly relied upon the detection of their anatomical position ( 2 , 3 ). (frontiersin.org)
  • Type I interferons, on the one hand, limit viral replication and thereby help the immune cells to control the viral pathogen. (eurekalert.org)
  • We verified that therapeutic treatment of acute viral hepatitis with type I interferon is reasonable, since it activates local immune cells and helps to eliminate the virus," concludes institute director Prof. Ulrich Kalinke. (eurekalert.org)
  • We also aimed to assess how KCs influence the other immune cells involved in instructing immune responses to such agents. (europa.eu)
  • The possible pathophysiological roles of hepatic immune cells, such as the two subsets of Kupffer cells/macrophages, in NAFLD/NASH induced by dietary cholesterol overload remain largely unknown. (deepdyve.com)
  • MSCs exert powerful immunosuppressive functions via paracrine effects and cell-cell contact-dependent interactions, and MSCs also inhibit the proliferation of activated lymphocytes, thereby providing survival signals to resting immune cells and subsequently stimulating the induction of regulatory immune cells [ 12 ]. (biomedcentral.com)
  • At certain time points after KCs ablation, we performed flow cytometry to monitor the amount of hepatic infiltrating immune cells . (bvsalud.org)
  • This study supports the notion that KCs actively interact with immune cells and LSECs for maintaining immune response and liver homeostasis . (bvsalud.org)
  • They are also useful in removing apoptotic cells from circulation. (wikipedia.org)
  • An efficient method to isolate Kupffer cells eliminating endothelial cell contamination and selective bias. (dbcls.jp)
  • We previously reported that gadolinium chloride (GdCl), an agent that depletes the liver of phagocytically active Kupffer cells, enhances hepatic expression of TNF messenger RNA (mRNA) and promotes liver regeneration after subsequent PH. (wiley.com)
  • Because IL-10 is known to shorten the half-life of TNF mRNA, these results suggest that Kupffer cell production of IL-10 is an important mechanism that down-regulates TNF production during liver regeneration. (wiley.com)
  • was assessed in culture supernatants by ELISA and that of nitric oxide (NO)by nitrate reductase method .5-LO mRNA expression was assessed by semiquantitative RT-PCR.RESULTS Vehicle-stimulated Kupffer cells produced a basal amount of NO,TNF? (bvsalud.org)
  • production and 5-LO mRNA expression by LPS-stlimulated Kupffer cells . (bvsalud.org)
  • Forskolin, the most potent inhibitor of LPS-induced nitrite formation by Kupffer cells, decreased iNOS mRNA levels in a time-dependent manner. (uthscsa.edu)
  • Time course studies indicated that forskolin was most effective at inhibiting LPS-induced nitrite formation and iNOS mRNA levels by Kupffer cells when added before LPS. (uthscsa.edu)
  • The exogenous tracer protein, which in contrast to endogenous peroxidase of Kupffer cells is not inhibited by prolonged aldehyde fixation, is taken up by micropinocytosis and remains confined to the lysosomal system of Kupffer cells. (rupress.org)
  • There are mainly two types of hepatic macrophages: Kupffer cells, that reside in the liver and originated from yolk sac-derived red bone marrow progenitor cells, and also monocyte-derived macrophages, derived from hematopoietic stem cells from the bone marrow and transported through the blood circulation to the liver. (wikipedia.org)

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