Substances that are recognized by the immune system and induce an immune reaction.
Substances elaborated by bacteria that have antigenic activity.
Proteins, glycoprotein, or lipoprotein moieties on surfaces of tumor cells that are usually identified by monoclonal antibodies. Many of these are of either embryonic or viral origin.
Antigens on surfaces of cells, including infectious or foreign cells or viruses. They are usually protein-containing groups on cell membranes or walls and may be isolated.
Substances elaborated by viruses that have antigenic activity.
Any part or derivative of any protozoan that elicits immunity; malaria (Plasmodium) and trypanosome antigens are presently the most frequently encountered.
Polyomavirus antigens which cause infection and cellular transformation. The large T antigen is necessary for the initiation of viral DNA synthesis, repression of transcription of the early region and is responsible in conjunction with the middle T antigen for the transformation of primary cells. Small T antigen is necessary for the completion of the productive infection cycle.
Antigens determined by leukocyte loci found on chromosome 6, the major histocompatibility loci in humans. They are polypeptides or glycoproteins found on most nucleated cells and platelets, determine tissue types for transplantation, and are associated with certain diseases.
Substances of fungal origin that have antigenic activity.
Differentiation antigens residing on mammalian leukocytes. CD stands for cluster of differentiation, which refers to groups of monoclonal antibodies that show similar reactivity with certain subpopulations of antigens of a particular lineage or differentiation stage. The subpopulations of antigens are also known by the same CD designation.
Any part or derivative of a helminth that elicits an immune reaction. The most commonly seen helminth antigens are those of the schistosomes.
The major group of transplantation antigens in the mouse.
A glycoprotein that is secreted into the luminal surface of the epithelia in the gastrointestinal tract. It is found in the feces and pancreaticobiliary secretions and is used to monitor the response to colon cancer treatment.
Those proteins recognized by antibodies from serum of animals bearing tumors induced by viruses; these proteins are presumably coded for by the nucleic acids of the same viruses that caused the neoplastic transformation.
A subclass of HLA-D antigens that consist of alpha and beta chains. The inheritance of HLA-DR antigens differs from that of the HLA-DQ ANTIGENS and HLA-DP ANTIGENS.
Molecules on the surface of T-lymphocytes that recognize and combine with antigens. The receptors are non-covalently associated with a complex of several polypeptides collectively called CD3 antigens (ANTIGENS, CD3). Recognition of foreign antigen and the major histocompatibility complex is accomplished by a single heterodimeric antigen-receptor structure, composed of either alpha-beta (RECEPTORS, ANTIGEN, T-CELL, ALPHA-BETA) or gamma-delta (RECEPTORS, ANTIGEN, T-CELL, GAMMA-DELTA) chains.
Antibodies produced by a single clone of cells.
Nuclear antigen with a role in DNA synthesis, DNA repair, and cell cycle progression. PCNA is required for the coordinated synthesis of both leading and lagging strands at the replication fork during DNA replication. PCNA expression correlates with the proliferation activity of several malignant and non-malignant cell types.
A group of antigens that includes both the major and minor histocompatibility antigens. The former are genetically determined by the major histocompatibility complex. They determine tissue type for transplantation and cause allograft rejections. The latter are systems of allelic alloantigens that can cause weak transplant rejection.
Large, transmembrane, non-covalently linked glycoproteins (alpha and beta). Both chains can be polymorphic although there is more structural variation in the beta chains. The class II antigens in humans are called HLA-D ANTIGENS and are coded by a gene on chromosome 6. In mice, two genes named IA and IE on chromosome 17 code for the H-2 antigens. The antigens are found on B-lymphocytes, macrophages, epidermal cells, and sperm and are thought to mediate the competence of and cellular cooperation in the immune response. The term IA antigens used to refer only to the proteins encoded by the IA genes in the mouse, but is now used as a generic term for any class II histocompatibility antigen.
A glycoprotein that is a kallikrein-like serine proteinase and an esterase, produced by epithelial cells of both normal and malignant prostate tissue. It is an important marker for the diagnosis of prostate cancer.
The lipopolysaccharide-protein somatic antigens, usually from gram-negative bacteria, important in the serological classification of enteric bacilli. The O-specific chains determine the specificity of the O antigens of a given serotype. O antigens are the immunodominant part of the lipopolysaccharide molecule in the intact bacterial cell. (From Singleton & Sainsbury, Dictionary of Microbiology and Molecular Biology, 2d ed)
IMMUNOGLOBULINS on the surface of B-LYMPHOCYTES. Their MESSENGER RNA contains an EXON with a membrane spanning sequence, producing immunoglobulins in the form of type I transmembrane proteins as opposed to secreted immunoglobulins (ANTIBODIES) which do not contain the membrane spanning segment.
Sites on an antigen that interact with specific antibodies.
A trisaccharide antigen expressed on glycolipids and many cell-surface glycoproteins. In the blood the antigen is found on the surface of NEUTROPHILS; EOSINOPHILS; and MONOCYTES. In addition, CD15 antigen is a stage-specific embryonic antigen.
Carbohydrate antigens expressed by malignant tissue. They are useful as tumor markers and are measured in the serum by means of a radioimmunoassay employing monoclonal antibodies.
A specific HLA-A surface antigen subtype. Members of this subtype contain alpha chains that are encoded by the HLA-A*02 allele family.
Lymphocytes responsible for cell-mediated immunity. Two types have been identified - cytotoxic (T-LYMPHOCYTES, CYTOTOXIC) and helper T-lymphocytes (T-LYMPHOCYTES, HELPER-INDUCER). They are formed when lymphocytes circulate through the THYMUS GLAND and differentiate to thymocytes. When exposed to an antigen, they divide rapidly and produce large numbers of new T cells sensitized to that antigen.
Differentiation antigens found on thymocytes and on cytotoxic and suppressor T-lymphocytes. CD8 antigens are members of the immunoglobulin supergene family and are associative recognition elements in MHC (Major Histocompatibility Complex) Class I-restricted interactions.
An immunoassay utilizing an antibody labeled with an enzyme marker such as horseradish peroxidase. While either the enzyme or the antibody is bound to an immunosorbent substrate, they both retain their biologic activity; the change in enzyme activity as a result of the enzyme-antibody-antigen reaction is proportional to the concentration of the antigen and can be measured spectrophotometrically or with the naked eye. Many variations of the method have been developed.
The major immunoglobulin isotype class in normal human serum. There are several isotype subclasses of IgG, for example, IgG1, IgG2A, and IgG2B.
Sets of cell surface antigens located on BLOOD CELLS. They are usually membrane GLYCOPROTEINS or GLYCOLIPIDS that are antigenically distinguished by their carbohydrate moieties.
Those hepatitis B antigens found on the surface of the Dane particle and on the 20 nm spherical and tubular particles. Several subspecificities of the surface antigen are known. These were formerly called the Australia antigen.
Complex of at least five membrane-bound polypeptides in mature T-lymphocytes that are non-covalently associated with one another and with the T-cell receptor (RECEPTORS, ANTIGEN, T-CELL). The CD3 complex includes the gamma, delta, epsilon, zeta, and eta chains (subunits). When antigen binds to the T-cell receptor, the CD3 complex transduces the activating signals to the cytoplasm of the T-cell. The CD3 gamma and delta chains (subunits) are separate from and not related to the gamma/delta chains of the T-cell receptor (RECEPTORS, ANTIGEN, T-CELL, GAMMA-DELTA).
Serological reactions in which an antiserum against one antigen reacts with a non-identical but closely related antigen.
Polymorphic class I human histocompatibility (HLA) surface antigens present on almost all nucleated cells. At least 20 antigens have been identified which are encoded by the A locus of multiple alleles on chromosome 6. They serve as targets for T-cell cytolytic responses and are involved with acceptance or rejection of tissue/organ grafts.
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
A CELL CYCLE and tumor growth marker which can be readily detected using IMMUNOCYTOCHEMISTRY methods. Ki-67 is a nuclear antigen present only in the nuclei of cycling cells.
Membrane glycoproteins consisting of an alpha subunit and a BETA 2-MICROGLOBULIN beta subunit. In humans, highly polymorphic genes on CHROMOSOME 6 encode the alpha subunits of class I antigens and play an important role in determining the serological specificity of the surface antigen. Class I antigens are found on most nucleated cells and are generally detected by their reactivity with alloantisera. These antigens are recognized during GRAFT REJECTION and restrict cell-mediated lysis of virus-infected cells.
Morphologic alteration of small B LYMPHOCYTES or T LYMPHOCYTES in culture into large blast-like cells able to synthesize DNA and RNA and to divide mitotically. It is induced by INTERLEUKINS; MITOGENS such as PHYTOHEMAGGLUTININS, and by specific ANTIGENS. It may also occur in vivo as in GRAFT REJECTION.
Human immune-response or Class II antigens found mainly, but not exclusively, on B-lymphocytes and produced from genes of the HLA-D locus. They are extremely polymorphic families of glycopeptides, each consisting of two chains, alpha and beta. This group of antigens includes the -DR, -DQ and -DP designations, of which HLA-DR is most studied; some of these glycoproteins are associated with certain diseases, possibly of immune etiology.
The property of antibodies which enables them to react with some ANTIGENIC DETERMINANTS and not with others. Specificity is dependent on chemical composition, physical forces, and molecular structure at the binding site.
Molecules on the surface of B- and T-lymphocytes that recognize and combine with specific antigens.
High-molecular weight glycoproteins uniquely expressed on the surface of LEUKOCYTES and their hemopoietic progenitors. They contain a cytoplasmic protein tyrosine phosphatase activity which plays a role in intracellular signaling from the CELL SURFACE RECEPTORS. The CD45 antigens occur as multiple isoforms that result from alternative mRNA splicing and differential usage of three exons.
Antigens of the virion of the HEPATITIS B VIRUS or the Dane particle, its surface (HEPATITIS B SURFACE ANTIGENS), core (HEPATITIS B CORE ANTIGENS), and other associated antigens, including the HEPATITIS B E ANTIGENS.
55-kDa antigens found on HELPER-INDUCER T-LYMPHOCYTES and on a variety of other immune cell types. CD4 antigens are members of the immunoglobulin supergene family and are implicated as associative recognition elements in MAJOR HISTOCOMPATIBILITY COMPLEX class II-restricted immune responses. On T-lymphocytes they define the helper/inducer subset. CD4 antigens also serve as INTERLEUKIN-15 receptors and bind to the HIV receptors, binding directly to the HIV ENVELOPE PROTEIN GP120.
Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
The processes triggered by interactions of ANTIBODIES with their ANTIGENS.
Glycoproteins expressed on cortical thymocytes and on some dendritic cells and B-cells. Their structure is similar to that of MHC Class I and their function has been postulated as similar also. CD1 antigens are highly specific markers for human LANGERHANS CELLS.
Serum that contains antibodies. It is obtained from an animal that has been immunized either by ANTIGEN injection or infection with microorganisms containing the antigen.
The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.
Immunoglobulins produced in a response to BACTERIAL ANTIGENS.
The production of ANTIBODIES by proliferating and differentiated B-LYMPHOCYTES under stimulation by ANTIGENS.
Established cell cultures that have the potential to propagate indefinitely.
Class I human histocompatibility (HLA) surface antigens encoded by more than 30 detectable alleles on locus B of the HLA complex, the most polymorphic of all the HLA specificities. Several of these antigens (e.g., HLA-B27, -B7, -B8) are strongly associated with predisposition to rheumatoid and other autoimmune disorders. Like other class I HLA determinants, they are involved in the cellular immune reactivity of cytolytic T lymphocytes.
Antigens expressed primarily on the membranes of living cells during sequential stages of maturation and differentiation. As immunologic markers they have high organ and tissue specificity and are useful as probes in studies of normal cell development as well as neoplastic transformation.
Deliberate stimulation of the host's immune response. ACTIVE IMMUNIZATION involves administration of ANTIGENS or IMMUNOLOGIC ADJUVANTS. PASSIVE IMMUNIZATION involves administration of IMMUNE SERA or LYMPHOCYTES or their extracts (e.g., transfer factor, immune RNA) or transplantation of immunocompetent cell producing tissue (thymus or bone marrow).
Lymphoid cells concerned with humoral immunity. They are short-lived cells resembling bursa-derived lymphocytes of birds in their production of immunoglobulin upon appropriate stimulation.
A melanosome-specific protein that plays a role in the expression, stability, trafficking, and processing of GP100 MELANOMA ANTIGEN, which is critical to the formation of Stage II MELANOSOMES. The protein is used as an antigen marker for MELANOMA cells.
Antigens associated with specific proteins of the human adult T-cell immunodeficiency virus (HIV); also called HTLV-III-associated and lymphadenopathy-associated virus (LAV) antigens.
A costimulatory ligand expressed by ANTIGEN-PRESENTING CELLS that binds to CTLA-4 ANTIGEN with high specificity and to CD28 ANTIGEN with low specificity. The interaction of CD80 with CD28 ANTIGEN provides a costimulatory signal to T-LYMPHOCYTES, while its interaction with CTLA-4 ANTIGEN may play a role in inducing PERIPHERAL TOLERANCE.
Immunologic techniques based on the use of: (1) enzyme-antibody conjugates; (2) enzyme-antigen conjugates; (3) antienzyme antibody followed by its homologous enzyme; or (4) enzyme-antienzyme complexes. These are used histologically for visualizing or labeling tissue specimens.
Nuclear antigens encoded by VIRAL GENES found in HUMAN HERPESVIRUS 4. At least six nuclear antigens have been identified.
Differentiation antigens expressed on B-lymphocytes and B-cell precursors. They are involved in regulation of B-cell proliferation.
Antigens stimulating the formation of, or combining with heterophile antibodies. They are cross-reacting antigens found in phylogenetically unrelated species.
The sum of the weight of all the atoms in a molecule.
The hepatitis B antigen within the core of the Dane particle, the infectious hepatitis virion.
An encapsulated lymphatic organ through which venous blood filters.
Technique involving the diffusion of antigen or antibody through a semisolid medium, usually agar or agarose gel, with the result being a precipitin reaction.
Immunoglobulin molecules having a specific amino acid sequence by virtue of which they interact only with the ANTIGEN (or a very similar shape) that induced their synthesis in cells of the lymphoid series (especially PLASMA CELLS).
A member of the tumor necrosis factor receptor superfamily with specificity for CD40 LIGAND. It is found on mature B-LYMPHOCYTES and some EPITHELIAL CELLS, lymphoid DENDRITIC CELLS. Evidence suggests that CD40-dependent activation of B-cells is important for generation of memory B-cells within the germinal centers. Mutations of the gene for CD40 antigen result in HYPER-IGM IMMUNODEFICIENCY SYNDROME, TYPE 3. Signaling of the receptor occurs through its association with TNF RECEPTOR-ASSOCIATED FACTORS.
Endogenous tissue constituents that have the ability to interact with AUTOANTIBODIES and cause an immune response.
The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.
A class of immunoglobulin bearing mu chains (IMMUNOGLOBULIN MU-CHAINS). IgM can fix COMPLEMENT. The name comes from its high molecular weight and originally being called a macroglobulin.
Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others.
A group of differentiation surface antigens, among the first to be discovered on thymocytes and T-lymphocytes. Originally identified in the mouse, they are also found in other species including humans, and are expressed on brain neurons and other cells.
A glycolipid, cross-species antigen that induces production of antisheep hemolysin. It is present on the tissue cells of many species but absent in humans. It is found in many infectious agents.
The complex formed by the binding of antigen and antibody molecules. The deposition of large antigen-antibody complexes leading to tissue damage causes IMMUNE COMPLEX DISEASES.
A sex-specific cell surface antigen produced by the sex-determining gene of the Y chromosome in mammals. It causes syngeneic grafts from males to females to be rejected and interacts with somatic elements of the embryologic undifferentiated gonad to produce testicular organogenesis.
Technique using an instrument system for making, processing, and displaying one or more measurements on individual cells obtained from a cell suspension. Cells are usually stained with one or more fluorescent dyes specific to cell components of interest, e.g., DNA, and fluorescence of each cell is measured as it rapidly transverses the excitation beam (laser or mercury arc lamp). Fluorescence provides a quantitative measure of various biochemical and biophysical properties of the cell, as well as a basis for cell sorting. Other measurable optical parameters include light absorption and light scattering, the latter being applicable to the measurement of cell size, shape, density, granularity, and stain uptake.
A heterogeneous group of immunocompetent cells that mediate the cellular immune response by processing and presenting antigens to the T-cells. Traditional antigen-presenting cells include MACROPHAGES; DENDRITIC CELLS; LANGERHANS CELLS; and B-LYMPHOCYTES. FOLLICULAR DENDRITIC CELLS are not traditional antigen-presenting cells, but because they hold antigen on their cell surface in the form of IMMUNE COMPLEXES for B-cell recognition they are considered so by some authors.
The species Oryctolagus cuniculus, in the family Leporidae, order LAGOMORPHA. Rabbits are born in burrows, furless, and with eyes and ears closed. In contrast with HARES, rabbits have 22 chromosome pairs.
Specialized cells of the hematopoietic system that have branch-like extensions. They are found throughout the lymphatic system, and in non-lymphoid tissues such as SKIN and the epithelia of the intestinal, respiratory, and reproductive tracts. They trap and process ANTIGENS, and present them to T-CELLS, thereby stimulating CELL-MEDIATED IMMUNITY. They are different from the non-hematopoietic FOLLICULAR DENDRITIC CELLS, which have a similar morphology and immune system function, but with respect to humoral immunity (ANTIBODY PRODUCTION).
Serologic tests based on inactivation of complement by the antigen-antibody complex (stage 1). Binding of free complement can be visualized by addition of a second antigen-antibody system such as red cells and appropriate red cell antibody (hemolysin) requiring complement for its completion (stage 2). Failure of the red cells to lyse indicates that a specific antigen-antibody reaction has taken place in stage 1. If red cells lyse, free complement is present indicating no antigen-antibody reaction occurred in stage 1.
A group of the D-related HLA antigens found to differ from the DR antigens in genetic locus and therefore inheritance. These antigens are polymorphic glycoproteins comprising alpha and beta chains and are found on lymphoid and other cells, often associated with certain diseases.
An albumin obtained from the white of eggs. It is a member of the serpin superfamily.
A costimulatory ligand expressed by ANTIGEN-PRESENTING CELLS that binds to CD28 ANTIGEN with high specificity and to CTLA-4 ANTIGEN with low specificity. The interaction of CD86 with CD28 ANTIGEN provides a stimulatory signal to T-LYMPHOCYTES, while its interaction with CTLA-4 ANTIGEN may play a role in inducing PERIPHERAL TOLERANCE.
A species of POLYOMAVIRUS originally isolated from Rhesus monkey kidney tissue. It produces malignancy in human and newborn hamster kidney cell cultures.
Immunized T-lymphocytes which can directly destroy appropriate target cells. These cytotoxic lymphocytes may be generated in vitro in mixed lymphocyte cultures (MLC), in vivo during a graft-versus-host (GVH) reaction, or after immunization with an allograft, tumor cell or virally transformed or chemically modified target cell. The lytic phenomenon is sometimes referred to as cell-mediated lympholysis (CML). These CD8-positive cells are distinct from NATURAL KILLER CELLS and NATURAL KILLER T-CELLS. There are two effector phenotypes: TC1 and TC2.
Proteins prepared by recombinant DNA technology.
T-cell receptors composed of CD3-associated alpha and beta polypeptide chains and expressed primarily in CD4+ or CD8+ T-cells. Unlike immunoglobulins, the alpha-beta T-cell receptors recognize antigens only when presented in association with major histocompatibility (MHC) molecules.
Histochemical localization of immunoreactive substances using labeled antibodies as reagents.
Immunoglobulins produced in response to VIRAL ANTIGENS.
A technique that combines protein electrophoresis and double immunodiffusion. In this procedure proteins are first separated by gel electrophoresis (usually agarose), then made visible by immunodiffusion of specific antibodies. A distinct elliptical precipitin arc results for each protein detectable by the antisera.
Manifestations of the immune response which are mediated by antigen-sensitized T-lymphocytes via lymphokines or direct cytotoxicity. This takes place in the absence of circulating antibody or where antibody plays a subordinate role.
An inhibitory T CELL receptor that is closely related to CD28 ANTIGEN. It has specificity for CD80 ANTIGEN and CD86 ANTIGEN and acts as a negative regulator of peripheral T cell function. CTLA-4 antigen is believed to play role in inducing PERIPHERAL TOLERANCE.
White blood cells formed in the body's lymphoid tissue. The nucleus is round or ovoid with coarse, irregularly clumped chromatin while the cytoplasm is typically pale blue with azurophilic (if any) granules. Most lymphocytes can be classified as either T or B (with subpopulations of each), or NATURAL KILLER CELLS.
The major interferon produced by mitogenically or antigenically stimulated LYMPHOCYTES. It is structurally different from TYPE I INTERFERON and its major activity is immunoregulation. It has been implicated in the expression of CLASS II HISTOCOMPATIBILITY ANTIGENS in cells that do not normally produce them, leading to AUTOIMMUNE DISEASES.
A component of the B-cell antigen receptor that is involved in B-cell antigen receptor heavy chain transport to the PLASMA MEMBRANE. It is expressed almost exclusively in B-LYMPHOCYTES and serves as a useful marker for B-cell NEOPLASMS.
Immunoglobulins produced in a response to PROTOZOAN ANTIGENS.
Electrophoresis in which a polyacrylamide gel is used as the diffusion medium.
Sialylated Lewis blood group carbohydrate antigen found in many adenocarcinomas of the digestive tract, especially pancreatic tumors.
A critical subpopulation of T-lymphocytes involved in the induction of most immunological functions. The HIV virus has selective tropism for the T4 cell which expresses the CD4 phenotypic marker, a receptor for HIV. In fact, the key element in the profound immunosuppression seen in HIV infection is the depletion of this subset of T-lymphocytes.
A critical subpopulation of regulatory T-lymphocytes involved in MHC Class I-restricted interactions. They include both cytotoxic T-lymphocytes (T-LYMPHOCYTES, CYTOTOXIC) and CD8+ suppressor T-lymphocytes.
Sensitive tests to measure certain antigens, antibodies, or viruses, using their ability to agglutinate certain erythrocytes. (From Stedman, 26th ed)
A melanosome-associated protein that plays a role in the maturation of the MELANOSOME.
Diagnostic procedures involving immunoglobulin reactions.
A group of dominantly and independently inherited antigens associated with the ABO blood factors. They are glycolipids present in plasma and secretions that may adhere to the erythrocytes. The phenotype Le(b) is the result of the interaction of the Le gene Le(a) with the genes for the ABO blood groups.
Genetically identical individuals developed from brother and sister matings which have been carried out for twenty or more generations, or by parent x offspring matings carried out with certain restrictions. All animals within an inbred strain trace back to a common ancestor in the twentieth generation.
Binary classification measures to assess test results. Sensitivity or recall rate is the proportion of true positives. Specificity is the probability of correctly determining the absence of a condition. (From Last, Dictionary of Epidemiology, 2d ed)
Immunoglobulins produced in a response to HELMINTH ANTIGENS.
Antigens which may directly stimulate B lymphocytes without the cooperation of T lymphocytes.
The rate dynamics in chemical or physical systems.
Glycoprotein members of the immunoglobulin superfamily which participate in T-cell adhesion and activation. They are expressed on most peripheral T-lymphocytes, natural killer cells, and thymocytes, and function as co-receptors or accessory molecules in the T-cell receptor complex.
The specific failure of a normally responsive individual to make an immune response to a known antigen. It results from previous contact with the antigen by an immunologically immature individual (fetus or neonate) or by an adult exposed to extreme high-dose or low-dose antigen, or by exposure to radiation, antimetabolites, antilymphocytic serum, etc.
A closely related group of antigens found in the plasma only during the infective phase of hepatitis B or in virulent chronic hepatitis B, probably indicating active virus replication; there are three subtypes which may exist in a complex with immunoglobulins G.
An increased reactivity to specific antigens mediated not by antibodies but by cells.
The phenomenon of target cell destruction by immunologically active effector cells. It may be brought about directly by sensitized T-lymphocytes or by lymphoid or myeloid "killer" cells, or it may be mediated by cytotoxic antibody, cytotoxic factor released by lymphoid cells, or complement.
Costimulatory T-LYMPHOCYTE receptors that have specificity for CD80 ANTIGEN and CD86 ANTIGEN. Activation of this receptor results in increased T-cell proliferation, cytokine production and promotion of T-cell survival.
A tumor necrosis factor receptor subtype found in a variety of tissues and on activated LYMPHOCYTES. It has specificity for FAS LIGAND and plays a role in regulation of peripheral immune responses and APOPTOSIS. Multiple isoforms of the protein exist due to multiple ALTERNATIVE SPLICING. The activated receptor signals via a conserved death domain that associates with specific TNF RECEPTOR-ASSOCIATED FACTORS in the CYTOPLASM.
Carbohydrate antigen most commonly seen in tumors of the ovary and occasionally seen in breast, kidney, and gastrointestinal tract tumors and normal tissue. CA 125 is clearly tumor-associated but not tumor-specific.
Antibodies that react with self-antigens (AUTOANTIGENS) of the organism that produced them.
Immunologically detectable substances found in the CELL NUCLEUS.
Allelic alloantigens often responsible for weak graft rejection in cases when (major) histocompatibility has been established by standard tests. In the mouse they are coded by more than 500 genes at up to 30 minor histocompatibility loci. The most well-known minor histocompatibility antigen in mammals is the H-Y antigen.
Members of the class of compounds composed of AMINO ACIDS joined together by peptide bonds between adjacent amino acids into linear, branched or cyclical structures. OLIGOPEPTIDES are composed of approximately 2-12 amino acids. Polypeptides are composed of approximately 13 or more amino acids. PROTEINS are linear polypeptides that are normally synthesized on RIBOSOMES.
A group of genetically identical cells all descended from a single common ancestral cell by mitosis in eukaryotes or by binary fission in prokaryotes. Clone cells also include populations of recombinant DNA molecules all carrying the same inserted sequence. (From King & Stansfield, Dictionary of Genetics, 4th ed)
Cells artificially created by fusion of activated lymphocytes with neoplastic cells. The resulting hybrid cells are cloned and produce pure MONOCLONAL ANTIBODIES or T-cell products, identical to those produced by the immunologically competent parent cell.
A specific HLA-B surface antigen subtype. Members of this subtype contain alpha chains that are encoded by the HLA-B*27 allele family.
The genetic region which contains the loci of genes which determine the structure of the serologically defined (SD) and lymphocyte-defined (LD) TRANSPLANTATION ANTIGENS, genes which control the structure of the IMMUNE RESPONSE-ASSOCIATED ANTIGENS, HUMAN; the IMMUNE RESPONSE GENES which control the ability of an animal to respond immunologically to antigenic stimuli, and genes which determine the structure and/or level of the first four components of complement.
A technique using antibodies for identifying or quantifying a substance. Usually the substance being studied serves as antigen both in antibody production and in measurement of antibody by the test substance.
Antigens produced by various strains of HEPATITIS D VIRUS.
Class I human histocompatibility (HLA) antigens encoded by a small cluster of structural genes at the C locus on chromosome 6. They have significantly lower immunogenicity than the HLA-A and -B determinants and are therefore of minor importance in donor/recipient crossmatching. Their primary role is their high-risk association with certain disease manifestations (e.g., spondylarthritis, psoriasis, multiple myeloma).
Glycoproteins with a wide distribution on hematopoietic and non-hematopoietic cells and strongly expressed on macrophages. CD58 mediates cell adhesion by binding to CD2; (ANTIGENS, CD2); and this enhances antigen-specific T-cell activation.
A major histocompatibily complex class I-like protein that plays a unique role in the presentation of lipid ANTIGENS to NATURAL KILLER T-CELLS.
A specific HLA-A surface antigen subtype. Members of this subtype contain alpha chains that are encoded by the HLA-A*01 allele family.
The insertion of recombinant DNA molecules from prokaryotic and/or eukaryotic sources into a replicating vehicle, such as a plasmid or virus vector, and the introduction of the resultant hybrid molecules into recipient cells without altering the viability of those cells.
Elements of limited time intervals, contributing to particular results or situations.
A specific HLA-B surface antigen subtype. Members of this subtype contain alpha chains that are encoded by the HLA-B*07 allele family.
Polysaccharides found in bacteria and in capsules thereof.
An HLA-DR antigen which is associated with HLA-DRB1 CHAINS encoded by DRB1*04 alleles.
Glycoproteins found on the membrane or surface of cells.
Laboratory mice that have been produced from a genetically manipulated EGG or EMBRYO, MAMMALIAN.
Tumors or cancer of the PROSTATE.
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
An HLA-DR antigen which is associated with HLA-DRB1 CHAINS encoded by DRB1*03 alleles.
The major human blood type system which depends on the presence or absence of two antigens A and B. Type O occurs when neither A nor B is present and AB when both are present. A and B are genetic factors that determine the presence of enzymes for the synthesis of certain glycoproteins mainly in the red cell membrane.
Tests that are dependent on the clumping of cells, microorganisms, or particles when mixed with specific antiserum. (From Stedman, 26th ed)
Cells grown in vitro from neoplastic tissue. If they can be established as a TUMOR CELL LINE, they can be propagated in cell culture indefinitely.
Small synthetic peptides that mimic surface antigens of pathogens and are immunogenic, or vaccines manufactured with the aid of recombinant DNA techniques. The latter vaccines may also be whole viruses whose nucleic acids have been modified.
Glycoproteins expressed on all mature T-cells, thymocytes, and a subset of mature B-cells. Antibodies specific for CD5 can enhance T-cell receptor-mediated T-cell activation. The B-cell-specific molecule CD72 is a natural ligand for CD5. (From Abbas et al., Cellular and Molecular Immunology, 2d ed, p156)
Glycoproteins found on immature hematopoietic cells and endothelial cells. They are the only molecules to date whose expression within the blood system is restricted to a small number of progenitor cells in the bone marrow.
Unglycosylated phosphoproteins expressed only on B-cells. They are regulators of transmembrane Ca2+ conductance and thought to play a role in B-cell activation and proliferation.
A member of the tumor necrosis factor receptor superfamily found on most T-LYMPHOCYTES. Activation of the receptor by CD70 ANTIGEN results in the increased proliferation of CD4-POSITIVE T-LYMPHOCYTES and CD8-POSITIVE T-LYMPHOCYTES. Signaling by the activated receptor occurs through its association with TNF RECEPTOR-ASSOCIATED FACTORS.
A specific HLA-A surface antigen subtype. Members of this subtype contain alpha chains that are encoded by the HLA-A*24 allele family.
Antigens that exist in alternative (allelic) forms in a single species. When an isoantigen is encountered by species members who lack it, an immune response is induced. Typical isoantigens are the BLOOD GROUP ANTIGENS.
Conjugated protein-carbohydrate compounds including mucins, mucoid, and amyloid glycoproteins.
Local surface sites on antibodies which react with antigen determinant sites on antigens (EPITOPES.) They are formed from parts of the variable regions of FAB FRAGMENTS.
Proteins found in any species of bacterium.
Vaccines or candidate vaccines designed to prevent or treat cancer. Vaccines are produced using the patient's own whole tumor cells as the source of antigens, or using tumor-specific antigens, often recombinantly produced.
The restriction of a characteristic behavior, anatomical structure or physical system, such as immune response; metabolic response, or gene or gene variant to the members of one species. It refers to that property which differentiates one species from another but it is also used for phylogenetic levels higher or lower than the species.
Administration of vaccines to stimulate the host's immune response. This includes any preparation intended for active immunological prophylaxis.
Substances that augment, stimulate, activate, potentiate, or modulate the immune response at either the cellular or humoral level. The classical agents (Freund's adjuvant, BCG, Corynebacterium parvum, et al.) contain bacterial antigens. Some are endogenous (e.g., histamine, interferon, transfer factor, tuftsin, interleukin-1). Their mode of action is either non-specific, resulting in increased immune responsiveness to a wide variety of antigens, or antigen-specific, i.e., affecting a restricted type of immune response to a narrow group of antigens. The therapeutic efficacy of many biological response modifiers is related to their antigen-specific immunoadjuvanticity.
Immunologic method used for detecting or quantifying immunoreactive substances. The substance is identified by first immobilizing it by blotting onto a membrane and then tagging it with labeled antibodies.
A specific immune response elicited by a specific dose of an immunologically active substance or cell in an organism, tissue, or cell.
They are oval or bean shaped bodies (1 - 30 mm in diameter) located along the lymphatic system.
Red blood cells. Mature erythrocytes are non-nucleated, biconcave disks containing HEMOGLOBIN whose function is to transport OXYGEN.
Classic quantitative assay for detection of antigen-antibody reactions using a radioactively labeled substance (radioligand) either directly or indirectly to measure the binding of the unlabeled substance to a specific antibody or other receptor system. Non-immunogenic substances (e.g., haptens) can be measured if coupled to larger carrier proteins (e.g., bovine gamma-globulin or human serum albumin) capable of inducing antibody formation.
Recombinant proteins produced by the GENETIC TRANSLATION of fused genes formed by the combination of NUCLEIC ACID REGULATORY SEQUENCES of one or more genes with the protein coding sequences of one or more genes.
The fission of a CELL. It includes CYTOKINESIS, when the CYTOPLASM of a cell is divided, and CELL NUCLEUS DIVISION.
Represents 15-20% of the human serum immunoglobulins, mostly as the 4-chain polymer in humans or dimer in other mammals. Secretory IgA (IMMUNOGLOBULIN A, SECRETORY) is the main immunoglobulin in secretions.
T-cell receptors composed of CD3-associated gamma and delta polypeptide chains and expressed primarily in CD4-/CD8- T-cells. The receptors appear to be preferentially located in epithelial sites and probably play a role in the recognition of bacterial antigens. The T-cell receptor gamma/delta chains are separate and not related to the gamma and delta chains which are subunits of CD3 (see ANTIGENS, CD3).
Differentiation antigens expressed on pluripotential hematopoietic cells, most human thymocytes, and a major subset of peripheral blood T-lymphocytes. They have been implicated in integrin-mediated cellular adhesion and as signalling receptors on T-cells.
Suspensions of attenuated or killed bacteria administered for the prevention or treatment of infectious bacterial disease.
Partial proteins formed by partial hydrolysis of complete proteins or generated through PROTEIN ENGINEERING techniques.
Immunoelectrophoresis in which a second electrophoretic transport is performed on the initially separated antigen fragments into an antibody-containing medium in a direction perpendicular to the first electrophoresis.
A HLA-DR antigen that is associated with HLA-DRB1 CHAINS encoded by DRB1*07 alleles.
Molecular products metabolized and secreted by neoplastic tissue and characterized biochemically in cells or body fluids. They are indicators of tumor stage and grade as well as useful for monitoring responses to treatment and predicting recurrence. Many chemical groups are represented including hormones, antigens, amino and nucleic acids, enzymes, polyamines, and specific cell membrane proteins and lipids.
In vitro method for producing large amounts of specific DNA or RNA fragments of defined length and sequence from small amounts of short oligonucleotide flanking sequences (primers). The essential steps include thermal denaturation of the double-stranded target molecules, annealing of the primers to their complementary sequences, and extension of the annealed primers by enzymatic synthesis with DNA polymerase. The reaction is efficient, specific, and extremely sensitive. Uses for the reaction include disease diagnosis, detection of difficult-to-isolate pathogens, mutation analysis, genetic testing, DNA sequencing, and analyzing evolutionary relationships.
Antigens from any of the hepatitis viruses including surface, core, and other associated antigens.
Proteins whose abnormal expression (gain or loss) are associated with the development, growth, or progression of NEOPLASMS. Some neoplasm proteins are tumor antigens (ANTIGENS, NEOPLASM), i.e. they induce an immune reaction to their tumor. Many neoplasm proteins have been characterized and are used as tumor markers (BIOMARKERS, TUMOR) when they are detectable in cells and body fluids as monitors for the presence or growth of tumors. Abnormal expression of ONCOGENE PROTEINS is involved in neoplastic transformation, whereas the loss of expression of TUMOR SUPPRESSOR PROTEINS is involved with the loss of growth control and progression of the neoplasm.
A malignant neoplasm derived from cells that are capable of forming melanin, which may occur in the skin of any part of the body, in the eye, or, rarely, in the mucous membranes of the genitalia, anus, oral cavity, or other sites. It occurs mostly in adults and may originate de novo or from a pigmented nevus or malignant lentigo. Melanomas frequently metastasize widely, and the regional lymph nodes, liver, lungs, and brain are likely to be involved. The incidence of malignant skin melanomas is rising rapidly in all parts of the world. (Stedman, 25th ed; from Rook et al., Textbook of Dermatology, 4th ed, p2445)
Domesticated bovine animals of the genus Bos, usually kept on a farm or ranch and used for the production of meat or dairy products or for heavy labor.
Multi-subunit proteins which function in IMMUNITY. They are produced by B LYMPHOCYTES from the IMMUNOGLOBULIN GENES. They are comprised of two heavy (IMMUNOGLOBULIN HEAVY CHAINS) and two light chains (IMMUNOGLOBULIN LIGHT CHAINS) with additional ancillary polypeptide chains depending on their isoforms. The variety of isoforms include monomeric or polymeric forms, and transmembrane forms (B-CELL ANTIGEN RECEPTORS) or secreted forms (ANTIBODIES). They are divided by the amino acid sequence of their heavy chains into five classes (IMMUNOGLOBULIN A; IMMUNOGLOBULIN D; IMMUNOGLOBULIN E; IMMUNOGLOBULIN G; IMMUNOGLOBULIN M) and various subclasses.
The lipid- and protein-containing, selectively permeable membrane that surrounds the cytoplasm in prokaryotic and eukaryotic cells.
Proteins which are found in membranes including cellular and intracellular membranes. They consist of two types, peripheral and integral proteins. They include most membrane-associated enzymes, antigenic proteins, transport proteins, and drug, hormone, and lectin receptors.
A species of gram-negative, facultatively anaerobic, rod-shaped bacteria (GRAM-NEGATIVE FACULTATIVELY ANAEROBIC RODS) commonly found in the lower part of the intestine of warm-blooded animals. It is usually nonpathogenic, but some strains are known to produce DIARRHEA and pyogenic infections. Pathogenic strains (virotypes) are classified by their specific pathogenic mechanisms such as toxins (ENTEROTOXIGENIC ESCHERICHIA COLI), etc.
Antibodies from an individual that react with ISOANTIGENS of another individual of the same species.
A specific HLA-A surface antigen subtype. Members of this subtype contain alpha chains that are encoded by the HLA-A*03 allele family.
The altered state of immunologic responsiveness resulting from initial contact with antigen, which enables the individual to produce antibodies more rapidly and in greater quantity in response to secondary antigenic stimulus.
An integrin alpha subunit of approximately 150-kDa molecular weight. It is expressed at high levels on monocytes and combines with CD18 ANTIGEN to form the cell surface receptor INTEGRIN ALPHAXBETA2. The subunit contains a conserved I-domain which is characteristic of several of alpha integrins.
The type species of LYMPHOCRYPTOVIRUS, subfamily GAMMAHERPESVIRINAE, infecting B-cells in humans. It is thought to be the causative agent of INFECTIOUS MONONUCLEOSIS and is strongly associated with oral hairy leukoplakia (LEUKOPLAKIA, HAIRY;), BURKITT LYMPHOMA; and other malignancies.
A measure of the binding strength between antibody and a simple hapten or antigen determinant. It depends on the closeness of stereochemical fit between antibody combining sites and antigen determinants, on the size of the area of contact between them, and on the distribution of charged and hydrophobic groups. It includes the concept of "avidity," which refers to the strength of the antigen-antibody bond after formation of reversible complexes.
The outward appearance of the individual. It is the product of interactions between genes, and between the GENOTYPE and the environment.
The uptake of naked or purified DNA by CELLS, usually meaning the process as it occurs in eukaryotic cells. It is analogous to bacterial transformation (TRANSFORMATION, BACTERIAL) and both are routinely employed in GENE TRANSFER TECHNIQUES.
The demonstration of the cytotoxic effect on a target cell of a lymphocyte, a mediator released by a sensitized lymphocyte, an antibody, or complement.
A single, unpaired primary lymphoid organ situated in the MEDIASTINUM, extending superiorly into the neck to the lower edge of the THYROID GLAND and inferiorly to the fourth costal cartilage. It is necessary for normal development of immunologic function early in life. By puberty, it begins to involute and much of the tissue is replaced by fat.
Genetic loci in the vertebrate major histocompatibility complex that encode polymorphic products which control the immune response to specific antigens. The genes are found in the HLA-D region in humans and in the I region in mice.
The process in which substances, either endogenous or exogenous, bind to proteins, peptides, enzymes, protein precursors, or allied compounds. Specific protein-binding measures are often used as assays in diagnostic assessments.

Epithelial hyperproliferation and transglutaminase 1 gene expression in Stevens-Johnson syndrome conjunctiva. (1/3039)

In Stevens-Johnson syndrome, pathological keratinization of the ordinarily nonkeratinized corneal and conjunctival mucosal epithelia results in severe visual loss. We examined conjunctiva covering cornea in five eyes in the chronic cicatricial phase of Stevens-Johnson syndrome. Normal conjunctiva from five age-matched individuals was studied also. The number of epithelial cells in Stevens-Johnson syndrome conjunctiva that were immunoreactive with a monoclonal antibody, Ki-67, to a nuclear antigen found only in proliferating cells was greater than normal (93.8+/-19.8 cells above 100 basal cells versus 12.8+/-0.5 cells above 100 basal cells; P = 0.009). In addition, although clinical inflammation was mild, massive lymphocytic infiltration was seen in the substantia propria of conjunctiva covering cornea. In situ hybridization documented transglutaminase 1 (keratinocyte transglutaminase) mRNA in suprabasal cells of the abnormally thickened conjunctival epithelium in all Stevens-Johnson syndrome patients. In contrast, no message was detected in normal conjunctival or corneal epithelia. Transglutaminase 1 is expressed during the terminal differentiation of keratinocytes where it helps synthesize cornified cell envelopes. We speculate that in Stevens-Johnson syndrome, epithelial hyperproliferation, and transglutaminase 1 gene expression lead to the pathological keratinization of ocular surface mucosal epithelia.  (+info)

Circadian variation in the expression of cell-cycle proteins in human oral epithelium. (2/3039)

At the tissue level, there is experimental and clinical data to suggest a cytokinetic coordination of the cell cycle with a greater proportion of cycling cells entering S-phase and mitosis at specific times of the day. The association of certain cell-cycle proteins with defined events in the cell cycle is well established and may be used to study the timing of cell-cycle phases over 24 hours. In this study oral mucosal biopsies were obtained from six normal human volunteers at 4-hour intervals, six times over 24 hours. Using immunohistochemistry, the number of positive cells expressing the proteins p53, cyclin-E, cyclin-A, cyclin-B1, and Ki-67 was determined for each biopsy and expressed as the number of positive cells per mm of basement membrane. We found a statistically significant circadian variation in the nuclear expression of all of these proteins with the high point of expression for p53 at 10:56 hours, cyclin-E at 14:59 hours, cyclin-A at 16:09 hours, cyclin-B1 at 21:13 hours, and Ki-67 at 02:50 hours. The circadian variation in the nuclear expression of cyclins-E (G1/S phase), -A (G2-phase), and -B1 (M-phase) with a normal physiological progression over time suggests a statistically significant circadian variation in oral epithelial cell proliferation. The finding of a circadian variation in the nuclear expression of p53 protein corresponding to late G1 is novel. This information has clinical implications regarding the timing of chemotherapy and radiotherapy.  (+info)

Immunologic proliferation marker Ki-S2 as prognostic indicator for lymph node-negative breast cancer. (3/3039)

BACKGROUND: Proper treatment of lymph node-negative breast cancer depends on an accurate prognosis. To improve prognostic models for this disease, we evaluated whether an immunohistochemical marker for proliferating cells, Ki-S2 (a monoclonal antibody that binds to a 100-kd nuclear protein expressed in S, G2, and M phases of the cell cycle), is an accurate indicator of prognosis. METHODS: We studied 371 Swedish women with lymph node-negative breast cancer; the median follow-up time was 95 months. The fraction of tumor cells in S phase was assessed by flow cytometry, and tumor cell proliferation was measured immunohistochemically with the monoclonal antibodies Ki-S2 and Ki-S5 (directed against the nuclear antigen Ki-67). A combined prognostic index was calculated on the basis of the S-phase fraction, progesterone receptor content, and tumor size. RESULTS: In multivariate analyses that did or did not (263 and 332 observations, respectively) include the S-phase fraction and the combined prognostic index, the Ki-S2 labeling index (percentage of antibody-stained tumor cell nuclei) emerged as the most statistically significant predictor of overall survival, disease-specific survival, and disease-free survival (all two-sided P<.0001). In the risk group defined by a Ki-S2 labeling index of 10% or less, life expectancy was not statistically significantly different from that of age-matched women without breast cancer, whereas the group with a high Ki-S2 labeling index had an increased risk of mortality of up to 20-fold. CONCLUSIONS: Cellular proliferation is a major determinant of the biologic behavior of breast cancer. Prognosis is apparently best indicated by the percentage of cells in S through M phases of the cell cycle. Measurement of the Ki-S2 labeling index of a tumor sample may improve a clinician's ability to make an accurate prognosis and to identify patients with a low risk of recurrence who may not need adjuvant therapy.  (+info)

Comparison of the Ki-67 score and S-phase fraction as prognostic variables in soft-tissue sarcoma. (4/3039)

Immunohistochemically determined Ki-67 scores and flow cytometrically determined S-phase fractions were successfully evaluated from the primary tumours of 123 patients with soft-tissue sarcoma. All patients had either limb or superficial trunk tumours. Ki-67 score correlated strongly with ploidy, S-phase fraction and grade. Ki-67 did not correlate with the size of the primary tumour. When analysed as a continuous variable, Ki-67 was a stronger predictor of both metastasis-free survival and disease-specific overall survival (P = 0.003 and 0.04 respectively) than was the S-phase fraction (P = 0.06 and 0.07 respectively). We tested the relevance of different cut-point values by dividing the whole material into two parts at every 10% (e.g. 10% of patients vs. the remaining 90%, 20% vs. 80%, etc.). We counted the relative risk and confidence interval at all these cut-off points. Ki-67 had good prognostic discriminating power irrespective of the cut-point value, but S-phase fraction lost its prognostic power at higher cut-point values. In conclusion, we found that Ki-67 is a useful prognostic tool in the treatment of soft-tissue sarcoma patients irrespective of the cut-point value. S-phase fraction can be used at lower cut-point values.  (+info)

Androgen receptor expression in male breast carcinoma: lack of clinicopathological association. (5/3039)

Androgen receptor (AR) expression was retrospectively analysed in 47 primary male breast carcinomas (MBCs) using a monoclonal antibody on formalin-fixed, paraffin-embedded tissues. AR immunopositivity was detected in 16 out of 47 (34%) cases. No association was found with patient age, tumour stage, progesterone receptor (PGR) or p53 protein expression. Well-differentiated MBCs tended to be AR positive more often than poorly differentiated ones (P = 0.08). A negative association was found between ARs and cell proliferative activity: MIB-1 scores were higher (25.4%) in AR-negative than in AR-positive cases (21.11%; P = 0.04). A strong positive association (P = 0.0001) was found between ARs and oestrogen receptors (ERs). In univariate analysis, ARs (as well as ERs and PGRs) were not correlated with overall survival; tumour histological grade (P = 0.02), size (P = 0.01), p53 expression (P = 0.0008) and MIB-1 scores (P = 0.0003) had strong prognostic value. In multivariate survival analysis, only p53 expression (P = 0.002) and histological grade (P = 0.02) retained independent prognostic significance. In conclusion, the lack of association between AR and most clinicopathological features and survival, together with the absence of prognostic value for ER/PGR status, suggest that MBCs are biologically different from female breast carcinomas and make it questionable to use antihormonal therapy for patients with MBC.  (+info)

Continuous perivascular L-arginine delivery increases total vessel area and reduces neointimal thickening after experimental balloon dilatation. (6/3039)

The aim of this study was to evaluate whether vascular remodeling and neointimal thickening occur after balloon dilatation of the nonatherosclerotic rabbit carotid artery, and whether both processes are influenced by continuous perivascular delivery of L-arginine or the nitric oxide synthase inhibitor NG-nitro-L-arginine methyl ester (L-NAME). In the first experiment, histological and morphometric evaluation of arteries was performed at different time points after balloon dilatation: 10 minutes (n=7), and 1 (n=7), 2 (n=9), 3 (n=20), or 10 (n=5) weeks. Neointimal thickening progressively contributed to luminal narrowing for at least 10 weeks after angioplasty. During the first 2 weeks after dilatation, a significant decrease of the total vessel area was measured. Ten weeks after dilatation, both the neointimal and total vessel area were increased without further changing of the luminal area. In the second experiment, endothelial injured rabbits were randomly assigned to receive 2 weeks of continuous local perivascular physiological salt solution (n=6), L-arginine (n=8), or L-NAME (n=7), starting immediately after balloon dilatation (ie, local drug delivery during the first phase of the biphasic vascular remodeling process). Perivascular L-arginine delivery significantly reduced the neointimal area, despite an increased number of neointimal Ki-67-positive smooth muscle cells. Both the luminal area and total vessel area were significantly increased. Serum L-arginine levels remained unchanged. L-NAME administration had no effect on the neointimal area, nor on the luminal and total vessel area. Neointimal formation and biphasic vascular remodeling occur after experimental balloon dilatation of the nonatherosclerotic rabbit carotid artery, and can be influenced by continuous local perivascular delivery of L-arginine.  (+info)

Tumor extension and cell proliferation in adenocarcinomas of the lung. (7/3039)

To elucidate the mechanism of tumor extension in human pulmonary adenocarcinoma, we immunohistochemically investigated the expression of cell cycle regulator proteins in 54 small adenocarcinomas less than 3 cm in diameter. The Ki-67-labeling index was significantly higher in the periphery of the tumor nodule than in the center. This proliferative potential correlated well with coexpression of cdk2 and cyclin A. p27, one of the cdk inhibitors, was highly expressed in normal bronchial epithelial cells. Peripherally located tumor cells expressed p27 at an intermediate level, but at a higher frequency and level than those in the center. Expression of p21 was also predominant in the periphery. Furthermore, the expression patterns of p21 and p27 were reciprocal. In vitro kinase assays further demonstrated higher cdk2 kinase activity in the periphery. These results suggest that: (i) within an emerging extension made up of peripherally located tumor cells, their high proliferative potential gradually wanes as their relative topographical position becomes more central in the expanding tumor; (ii) peripherally located tumor cells maintain their proliferative potential by higher cyclin A-cdk2 complex activity; and (iii) intermediate expression of p21/p27 in the peripherally located cells promotes higher cyclin A-cdk2 kinase activity, whereas high p21/p27 expression in nonneoplastic cells inhibits kinase activity.  (+info)

Comparison of mitotic index, in vitro bromodeoxyuridine labeling, and MIB-1 assays to quantitate proliferation in breast cancer. (8/3039)

PURPOSE: To investigate the hypothesis that in vitro bromodeoxyuridine (BrDu) labeling might be superior to MIB-1 immunostaining for prognostic value, because it more selectively labels cells during the S phase. METHODS: Four hundred eighty-six patients with breast cancers (59% lymph node-negative, 41% lymph node-positive) surgically excised between 1988 and 1993 (median follow-up, 62 months) were evaluated for cellular proliferation using prospective in vitro BrDu uptake assays, retrospective mitotic indices, and MIB-1 labeling. RESULTS: MIB-1, BrDu labeling, and mitotic index-derived proliferation data were highly correlated. Each was similarly associated with most other markers of prognosis, although these relationships were not identical. By univariate analysis, nodal status was the most significant prognostic variable for all patients. Higher BrDu labeling index, MIB-1 immunolabeling, and mitotic index were also associated with shortened disease-free survival (DFS) and disease-specific survival for the entire patient group, as well as for node-negative patients. The association between cellular proliferation and survival was much weaker for node-positive patients. Multivariate models confirmed that nodal status, tumor size, and proliferation data predicted survival in all patients as well as those with node-negative disease, although MIB-1 was somewhat more closely associated with outcome than mitotic index or in vitro BrDu data. For patients with T1NOMO disease (n = 172), the only significant predictors of DFS were proliferation rate (mitotic index or MIB-1) and tumor grade. CONCLUSIONS: Proliferation rate predicts recurrence and survival in breast cancer. This effect is more pronounced in node-negative patients. In vitro BrDu data are not superior to MIB-1 and mitotic counting.  (+info)

TY - JOUR. T1 - Independent prognostic significance of a nuclear proliferation antigen in diffuse large cell lymphomas as determined by the monoclonal antibody Ki-67. AU - Grogan, T. M.. AU - Lippman, S. M.. AU - Spier, C. M.. AU - Slymen, D. J.. AU - Rybski, J. A.. AU - Rangel, C. S.. AU - Richter, L. C.. AU - Miller, T. P.. PY - 1988. Y1 - 1988. N2 - To assess the prognostic significance of the growth fraction in diffuse large cell lymphoma (DLCL), we studied 105 DLCL patients with the monoclonal antibody Ki-67 applied to frozen tissue sections. Ki-67 detects a nuclear antigen associated with cell proliferation not found in resting cells. Ki-67 findings and other clinical prognostic factors were correlated with outcome using univariate and multivariate analyses in the proportional hazards model. High proliferative activity, defined as nuclear Ki-67 expression in , 60% of malignant cells (Ki-67 , 60), was found to be a strong predictor of poor survival among these patients (P = .003, log-rank). ...
The growth fractions of 160 mammary carcinomas and 30 benign mammary lesions were determined in situ by immunostaining with the monoclonal antibody Ki-67. Benign lesions had a mean value of 3% Ki-67 positive cells, whereas the mean value of mammary carcinomas was 16.6%. A comparison of the mean values of Ki-67 positive cells with the histological grade of the tumours showed a correlation between these two variables--that is, histological grade 1 showed 9%, grade 2 16%, and grade 3 26% proliferating cells. Considering the individual Ki-67 values in the different histological grades, it was evident that there was considerable scatter in the number of proliferating cells, so that the proliferation rates of grades 1, 2, and 3 overlapped each other. This indicates a dissociation between histological grade of malignancy and size of the growth fraction in most breast cancers. Follow up studies are needed to establish which of the two variables--that is, morphological degree of malignancy, or the ...
The expression of the cell proliferation marker, Id2, after the depletion of the GSH.The cells were plated as usual and after attaching, 100 µM DEM, or 10 mM B
PerCP/Cyanine5.5 anti-human Ki-67 Antibody - Antigen Ki-67 is a nuclear protein expressed as two isoforms with molecular weights of 395 and 345 kD.
Objective: To investigate the spatial and temporal expression of proliferation marker Ki-67 during early human tooth development, and to associate the process of cell proliferation with the advancement of different stages in tooth development. Design: Analysis of spatial and temporal appearance of Ki-67 proliferation marker in the tooth germ of human embryos and fetuses, and follow up of changes in percentages of proliferating cells in relation to different developmental stages or parts of the tooth germ. Place of investigation: Department of anatomy, histology and embryology, School of Medicine in Split, Laboratory for investigation of early human development. Materials and methods: In serial paraffin sections of 8 normal developing human teeth 5-10 postovulatory weeks old, immunohistochemical technique was performed using Ki-67 marker as primary antibody and diaminobenzidine (DAB) as secondary antibody. Number of Ki-67 positive cells was analyzed by using statistical tests (Mann-Whitney, ...
Ki67 antibody (antigen identified by monoclonal antibody Ki-67) for ICC/IF, IHC, IHC-P. Anti-Ki67 pAb (GTX20833) is tested in Human, Mouse, Rat samples. 100% Ab-Assurance.
3 months: 1mL. 3-24 months: 3mL. 25 months-18 years: 5mL. ,18 years: 20mL. Samples must be kept at room temperature. Specimen must be collected Mon-Thurs between 10am-2pm and received in laboratory by 3pm.. ...
Recent studies showed preliminary data on associations of MRI-based imaging phenotypes of breast tumours with breast cancer molecular, genomic, and related characteristics. In this study, we present a comprehensive analysis of this relationship. We analysed a set of 922 patients with invasive breast cancer and pre-operative MRI. The MRIs were analysed by a computer algorithm to extract 529 features of the tumour and the surrounding tissue. Machine-learning-based models based on the imaging features were trained using a portion of the data (461 patients) to predict the following molecular, genomic, and proliferation characteristics: tumour surrogate molecular subtype, oestrogen receptor, progesterone receptor and human epidermal growth factor status, as well as a tumour proliferation marker (Ki-67). Trained models were evaluated on the set of the remaining 461 patients. Multivariate models were predictive of Luminal A subtype with AUC = 0.697 (95% CI: 0.647-0.746, p | .0001), triple negative breast
Background. CTLA4 blocking monoclonal antibodies provide durable clinical benefit in a subset of patients with advanced melanoma mediated by intratumoral lymphocytic infiltrates. A key question is defining if the intratumoral infiltration is a differentiating factor between patients with and without tumor responses. Methods. Paired baseline and post-dosing tumor biopsies from 19 subjects, including three patients with an objective tumor response, were prospectively collected from patients with metastatic melanoma receiving the anti-CTLA4 antibody tremelimumab within a clinical trial with primary endpoint of quantitating CD8+ cytotoxic T lymphocyte (CTL) infiltration in tumors. Samples were analyzed for cell density using automated imaging capture, and further characterized for functional lymphocyte properties by assessing the cell activation markers HLA-DR and CD45RO, the cell proliferation marker Ki67 and the T regulatory cell marker FOXP3. Results. There was a highly significant increase in ...
The results of the analysis reported herein suggest that the Ki67 assay is able to significantly discriminate the long-term prognosis of patients with primary resected ILC. Indeed, Ki67 emerges as a significant prognostic factor in multivariate analysis and the best cut-offs able to discriminate between very good prognosis and poor prognosis seems to be 6 and 21 %. If very good performers (i.e. very low proliferation, Ki67 , 6 %) are excluded, the cut-off of 21 % is confirmed (and internal validated) as an independent predictor for longer DFS and OS.. The prognostic significance of Ki67, a well-established cell proliferation marker in cancer, have been extensively investigated in studies mainly conducted in IDC cases in order to provide early and accurate information upon both outcome and prediction of response to treatment. These studies have shown an independent significant association between high Ki67 expression and increased risk of breast cancer relapse and benefit of the addition of ...
H&E staining showed spindle-shaped tumor cells, many of which had mitotic figures and were positive for the proliferation marker Ki67 (Figure 3E and Supplemental Figure 7). Tumors contained many microvessels, slit-like spaces, and infiltrates of immune cells such as CD45R+ mouse B cells, which were reminiscent of CD8+ T cells and CD20+ B cells in KS tumors (Supplemental Figure 8). These results indicated that KMM tumors consisted of proliferating spindle cells with vast inflammatory infiltrates and neoangiogenesis. All tumor cells strongly expressed LANA protein, with 1%-3% of them expressing ORF65 protein (Figure 3E). Tumors had robust expression of latent LANA and vCyclin transcripts but heterogeneous expression of lytic transcripts (Supplemental Figure 9). RTA was at levels close to those in uninduced KMM cells in 2 of 14 tumors; ORF57 and ORF59 were at levels higher than in uninduced cells in 12 of 14 tumors; and ORF65 was detected in 5 of 14 tumors, of which only 1 was at a high level while ...
The expression of the human Ki-67 protein is strictly associated with cell proliferation. During interphase, the antigen can be exclusively detected within the nucleus, whereas in mitosis most of the protein is relocated to the surface of the chromosomes. The fact that the Ki-67 protein is present d …
Results: This study reports the eventual outcome of the 97 patients after 12 years. There were no significant associations between proliferation data of the index tumours and patient outcome. No adverse events were identified which could be attributed to the use of the halogenated pyrimidine label in vivo ...
Anti-Ki67 antibody [B126.1] (ab8191) has been cited in 19 publications. References for Human, Mouse in IF, IHC, IHC (Methanol fixed), IHC-Fr, IHC-P, WB
PA2G4 (proliferation-associated 2G4, 38kDa), Authors: Anne Hamburger, Arundhati Ghosh, Smita Awasthi. Published in: Atlas Genet Cytogenet Oncol Haematol.
The mean patient age was 64 years (range 49-86 years), and 50% of patients (7/14) were female. The average tumor volume was 8.07 cm3 (range 0.21-27.89 cm3). The median Ki-67 in the cohort was 15%. When stratified by median Ki-67, patients with Ki-67 greater than 15% had lower median v p (0.02 vs 0.10, p = 0.002), and lower median wash-in rate (1.27 vs 4.08 sec−1, p = 0.04) than patients with Ki-67 of 15% or below. Logistic regression analysis demonstrated a statistically significant, moderate positive correlation between v e and time to progression (r = 0.49, p , 0.05). Furthermore, there was a moderate positive correlation between K trans and time to progression, which approached, but did not reach, statistical significance (r = 0.48, p = 0.05). ...
If you know of any papers that use this antibody, please contact us at antibodies [at] alzforum [dot] org for consideration in the References section.. ...
DI-fusion, le Dépôt institutionnel numérique de lULB, est loutil de référencementde la production scientifique de lULB.Linterface de recherche DI-fusion permet de consulter les publications des chercheurs de lULB et les thèses qui y ont été défendues.
高い抗原親和性、特異性と安定した品質を兼ね備えたアブカムのウサギ・モノクローナル抗体 RabMAb® ab21700 交差種: Ms,Rat,Hu 適用: IHC-P,IHC-Fr
Summarizes information relating to this test including clinical use and background, individuals suitable for testing, specimen requirements, methodology, test interpretation, and peer-reviewed references.
If you know of any papers that use this antibody, please contact us at antibodies [at] alzforum [dot] org for consideration in the References section.. ...
产品名称:Anti-Mouse/RatKi-67(cloneSolA15)Purified规格:100ug货号:14-5698-82厂商:eBioscience产品介绍:HostRatIsotypeIgG2a,kappaReactivityCanine,Human,Mouse,RatConjugatePurifiedReportedApplicationsImmunohistochemicalSt
फरीदाबाद 24 सितम्बर। बल्लबगढ़ निवासी पंडित वेद वशिष्ठ को श्रीराम मंदिर निर्माण प्रयास ट्रस्ट का प्रदेश अध्यक्ष, हरियाणा नियुक्त किया है। उन्हें प्रदेश अध्यक्ष का दायित्व राष्ट्रीय संयोजक विनोद मुदगिल ने प्रदेश प्रभारी आनंद सिंह के अनुमोदन पर नियुक्त किया है। वेद वशिष्ठ ने प्रदेश अध्यक्ष बनने पर ट्रस
फरीदाबाद। कोरोना वायरस की वजह से देश भर में 21 दिन का लॉक डाउन है. जरूरी सामान की दुकानें और सेवाओं को छोडक़र सब कुछ बंद है. इसका सबसे ज्यादा असर रोजाना कमाने वाले गरीब देहाड़ी मजदूरों पर पड़ा है. कई लोग और परिवार ऐसे हैं जिनके सामने रोजी-रोटी का संकट खड़ा हो गया है। जिस कारण कई लोग भोजन व राशन की कि
Background: Dentigerous cyst is the most common type of developmental odontogenic cyst which originates from REE. It has the capacity for transformation to ameloblastoma, mucoepidermoid carcinoma and squamous cell carcinoma. ki-67 and PCNA are two cell proliferation markers which can be both detected in cysts and tumors of epithelial origin. ...
The present pharmacokinetic study is part of a clinical trial that investigated the effects of normal dose or low-dose tamoxifen regimens on the expression of the proliferation antigen Ki-67 and several circulating surrogate end point biomarkers (24) . Patients were randomly allocated to either the conventional dose of 20 mg/day, or 5 mg/day or 1 mg/day for 28 days before surgery. The post-treatment levels of Ki-67 were significantly lower in the tamoxifen-treated groups compared with the control groups, but there was no evidence of a dose-response relationship (24) .. A main finding of the present study is that the tamoxifen and metabolite concentrations in breast cancer tissue, normal breast tissue, and serum were inter-related. The average concentrations of tamoxifen in serum approximated to the estimated values, indicating linear pharmacokinetics, even in the low-dose range. However, a ,10-fold variation of tamoxifen concentrations in serum was observed among subjects in each of the dosing ...
Primitive neuroectodermal tumor of the posterior fossa: medulloblastoma: evaluation of the proliferative index by means of monoclonal antibody Mib-1, its prognostic correlation and therapeutics implications ...
Today, it is time for the first image of the week from the Cancer Atlas!. Lung cancer is one of the deadliest and most common forms of human cancer. Different forms of lung cancer exist and non-small cell lung cancer is the most common form. The cancer image this week is selected from such a lung cancer that has been immunohistochemically stained for the proliferation marker Ki-67 (MKI67). Ki-67 is the most commonly used immunohistochemical marker in rutin cancer diagnostics. The Ki-67 antigen is expressed in all cell nuclei that are active in the cell cycle and thus positive staining in a tumor cell population reflects the level of proliferation in that particular cancer...Read more. ...
A growth inhibition endpoint action refers to growth reduction of a predefined stimulus. The unit of measure is always percent, when normalized to the dynamic range of the assay. Cell viability or proliferation can be measured in a variety of ways, e.g. 1) protein content by sulforhodamine B staining followed by absorbance measurement, 2) mitochondrial dehydrogenase activity by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrasodium bromide, MTT staining, followed by absorbance measurement, 3) expression of proliferation-associated antigens by immunostaining for Ki-67, and 4) ATP content by using CellTiter-Glo reagent (Promega) followed by luminescence measurement ...
Background: Growth hormone (GH) producing adenomas, frequently express several hormones. This condition could confer them a higher proliferative capacity. Ki-67 is a nuclear protein antigen that is a marker for proliferative activity. Aim: To measure the immunohistochemical hormone expression in pituitary adenomas, excised from patients with acromegaly. To determine if the pluríhormonal condition of these adenomas is associated with a higher proliferative capacity, assessed through the expression of Ki-67. Material and methods: Forty one paraffin embedded surgical samples of pituitary adenomas from patients with acromegalia were studied. Immunohistochemistry for GH, prolactin (PRL), follicle stimulating hormone (FSH), luteinizing hormone (LH), thyroid stimulating hormone (TSH), adrenocorticotropin (ACTH) and for the expression of Ki-67 was carried out. Results: All samples were positive for GH. Twenty seven had positive staining for PRL, 12 had positive staining for glycoproteic hormones and 11 ...
Aims: The aim was to demonstrate a method for automated image analysis of immunohistochemically stained tissue samples for extracting features that correlate with patient disease. We address the problem of quantifying tumor tissue and segmenting and counting cell nuclei. Materials and Methods: Our method utilizes a flexible segmentation method based on sparse coding trained from representative image samples. Nuclei counting is based on a nucleus model that takes size, shape, and nucleus probability into account. Nuclei clustering and overlays are resolved using a gray-weighted distance transform. We obtain a probability measure for pixels belonging to a nucleus from our segmentation procedure. Experiments are carried out on two sets of immunohistochemically stained images - one set based on the estrogen receptor (ER) and the other on antigen KI-67. For the nuclei separation we have selected 207 ER image samples from 58 tissue micro array-cores corresponding to 58 patients and 136 KI-67 image ...
In frozen breast cancers samples (n = 56), three distinct CDK4 modification profiles are observed using 2D‐gel electrophoresis: profile H, preponderant presence of T172‐phosphorylated CDK4 (most luminal B and HER2‐positive cancers); profile L, minor presence of phosphorylated CDK4 (90% of lower risk luminal A tumors); profile A, no phosphorylated CDK4 despite high proliferation index (60% of triple‐negative cancers). ...
Ki-67 nuclear antigen is associated with cell proliferation and is found throughout the cell cycle (G1, S, G2, M phases). It is never expressed by resting (G0) cells. The Ki-67 antigen is a human nuclear protein defined by its reactivity with monoclonal antibody from the Ki-67 clone. Two isoforms of 345 and 395 kDa have been identified by cDNA sequence coding. Deduced amino acid sequence analysis of the Ki-67 antigen confirmed that the cDNA encodes for a nuclear and short-lived protein without any significant homology to known sequences. The Ki-67 antigen is expressed at active phases of the cell cycle (G1, S, G2 and M phases), but it is absent in resting cells (G0 phase). The level of Ki-67 antigen expression varies during cell cycle and it has been correlated to several cell pathways: The Ki-67 decrease pathway is characterized by a declining Ki-67 staining and leads eventually to the exit from the active cell cycle (G0). If cells on this pathway get stimulated by growth factors, they can ...
The primary objective of the study was to evaluate the association between the minimum apparent diffusion coefficient (ADCmin) and Ki-67, an index for cellular proliferation, in non-small cell lung cancers. Also, we aimed to assess whether ADCmin values differ between tumour subtypes and tissue sampling method.. The patients who had diffusion weighted magnetic resonance imaging (DW-MRI) were enrolled retrospectively. The correlation between ADCmin and the Ki-67 index was evaluated.. Ninety three patients, with a mean age 65 ± 11 years, with histopathologically proven adenocarcinoma and squamous cell carcinoma of the lungs and had technically successful DW-MRI were included in the study. The numbers of tumour subtypes were 47 for adenocarcinoma and 46 for squamous cell carcinoma. There was a good negative correlation between ADCmin values and the Ki-67 proliferation index (r = -0.837, p , 0.001). The mean ADCmin value was higher and the mean Ki-67 index was lower in adenocarcinomas compared to ...
Ki-67, Biotin, clone: SolA15, eBioscience™ 25μg; Biotin Ki-67, Biotin, clone: SolA15, eBioscience™ Primary Antibodies K
pS2 content was not correlated with menopausal status, tumor size, nodal involvement or tumor proliferative activity expressed as labeling index (tritiated thymidine LI). The differential diagnosis of intracardiac masses (ICM) is wide. High risk of severe liver disease among the few who are infected by their mothers underlines the generic cialis reviews importance of interrupting perinatal transmission in sub-Saharan Africa. Cancer immunotherapy was deemed the medical breakthrough of 2013, in part because it can induce a rapid, durable, self-propagating and adaptable immune response. We find that after familiarizing subjects with a spatial sequence, flashing only the starting point of the sequence triggers an activity wave in V1 that resembles the full stimulus sequence. The incidence and mortality rates of cutaneous melanoma (CM) are increasing among fair-skinned populations worldwide.. Extraction and radicals scavenging activity generic cialis price comparison of polysaccharides with microwave ...
Proliferative index and effect of blueberry diet with or withoutestrogen (E2) treatment. (A) Immunohistochemical stainingfor proliferating cell nuclear antigen
Novel antibodies have been generated by immunizing with bacterially expressed fragments of the repetitive motif of the Ki-67 gene. One such antibody, MIB1, recognizes a fixation and embedding resistant epitope on the Ki-67 protein if sections are previously microwaved in a citrate buffer. We have in …
Baisch, H., Gerdes, J. Simultaneous staining of exponentially growing versus plateau phase cells with the proliferation-associated antibody Ki-67 and propidium iodide: analysis by flow cytometry. Cell and Tissue Kinetics, 1987 Jul, 20(4):387-91 ...
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Cell proliferation markers at the invasive tumor front of oral squamous cell carcinoma: comparative analysis in relation to clinicopathological parameters of ...
AIMS: Nerve growth factor (NGF) has been reported to prevent neuronal damage and contributes to the functional recovery in animal brain injury models and human ischemic disease as well. We aimed to investigate a potential therapeutic effect of NGF gene treatment in ischemic stroke and to estimate the functional recovery both at the cellular and cognitive levels in an ischemia rat model. METHODS: After microinjection of pseudolentivirus-delivered β-NGF into an established ischemic stroke model in rats (tMCAO), we estimated neuronal cell apoptosis with TUNEL labeling and neurogenesis by cell proliferation marker Ki67 staining in both ischemic core and penumbra of striatum ...
Ki67 Immunohistochemistry. Uncontrolled proliferation is a hallmark of cancer. In breast cancer, the most widely practiced method for comparing proliferation between tumor samples involves the immunohistochemical assessment of Ki67 antigen (also known as antigen identified by monoclonal antibody Ki-67 [MKI67]), a nuclear marker expressed in all phases of the cell cycle other than the G0 phase. Potential uses include prognosis, prediction of relative responsiveness or resistance to chemotherapy or endocrine therapy, estimation of residual risk in patients on standard therapy and as a dynamic biomarker of treatment efficacy in samples taken before, during, and after neoadjuvant therapy, particularly neoadjuvant endocrine therapy. Increasingly, Ki67 is measured in these scenarios for clinical research, including as a primary efficacy endpoint for clinical trials, and sometimes for clinical management.. Reference: Dowsett M, Nielsen TO, AHern R et al. Assessment of Ki67 in breast cancer: ...
TY - JOUR. T1 - Immunohistochemical status of the p53 protein and Ki-67 antigen using biopsied specimens can predict a sensitivity to neoadjuvant therapy in patients with esophageal cancer. AU - Kitamura, Kaoru. AU - Saeki, Hiroshi. AU - Kawaguchi, Hidetoshi. AU - Araki, Koshi. AU - Ohno, Shinji. AU - Kuwano, Hiroyuki. AU - Maehara, Yoshihiko. AU - Sugimachi, Keizo. PY - 2000/5/3. Y1 - 2000/5/3. N2 - Background/aims: The prediction of an effective adjuvant therapy is essential in order to increase the number of esophageal cancer cases demonstrating an excellent response. Correlation of the immunohistochemical expression of p53 protein and Ki-67 antigen were studied using biopsied specimens of advanced esophageal cancer. The combined staining status of both can help predict the efficacy of neoadjuvant therapy in patients with esophageal cancer. Methodology: The overexpression of p53 protein and the Ki-67 labeling percentage were immunohistochemically investigated in 95 biopsied specimens of ...
Hormone treatment with an estrogen plus a progestagen increases the risk of breast cancer. Both hormone activities reside also in tibolone. In order to assess the breast safety of tibolone the compound was evaluated in various preclinical models. In breast cancer cell lines the effects of tibolone were inconclusive, but in various in vivo models it did not stimulate the breast. In the DMBA model tibolone clearly inhibited the growth of breast tumors and when tibolone is given in a prophylactic design far less tumors develop. Ovariectomized monkeys treated for two years with tibolone show no increase in the expression of the proliferation marker Ki67. Several investigations were performed in order to unravel the mode of action of tibolone in breast tissue. The effects of tibolone and its metabolites on the various steroid metabolizing enzymes in breast tissues were therefore investigated. Tibolone or its metabolites did not inhibit the enzyme aromatase, but sulfatase was profoundly inhibited. The ...
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Background: Ki67 is a nuclear and nuclear protein antigen present in all proliferating cells during the active part of the cell cycle: G1, S, G2, and mitosis. The aim of study is to evaluate survival based on Ki67 index in NHL patients in the west of Iran for the first time. Patients and Methods: Between of 2002 to 2014, fifty-six patients with NHL referred to Our Clinic. We checked age, sex, type of NHL, Ki67 index and survival for them. We divided Ki67 index to two groups: low Ki67 (Ki67|65%) and high Ki67 (Ki67≥65%). Results: The mean age at diagnosis for patients was 47.33±166.50 years (range, 13-77 years) that 27 patients (48.2%) had age≤ 50 years and 33 patients (58.9%) were male. Thirty-eight patients (67.9%) had Ki67|60% and 18 patients (32.1%) had Ki67≥65%. The mean Ki67 for Nodal patients was 48.1% and for extra nodal was 54.5%, but there was no significant correlation between them (P=0.360). Conclusion: Ki67 in future studies should be divided based on a fix percent until we can have a
Cinthia B Drachenberg MD, Remy Blanchaert MD DDS, Olga B Ioffe MD, Robert A Ord MD DDS, John C Papadimitriou MD PhD. In verrucous carcinoma (VC) the apoptotic index consistently ranged from 0.1 to 1%, Ki-67 positive nuclei were confined to the basal epithelial layer, bcl-2 was expressed only in the cytoplasm of few scattered cells and p53 mutations were present in 50% of the lesions. In squamous cell carcinoma the apoptotic index ranged from 3 to 8 %, K-67 positive nuclei were randomly distributed, bcl-2 cytoplasmic and nuclear expression was more widespread and higher than in VC. p53 positive nuclei were identified in 62.5% of the lesions. ,,,. ...
Glioblastoma multiforme is the most frequent, aggressive and fatal type of brain tumor. Glioblastomas are characterized by their infiltrating nature, high proliferation rate and resistance to chemotherapy and radiation. Recently, oncologic therapy experienced a rapid evolution towards
We recently had a Cyno monkey study which required evaluation of cell proliferation in formalin fixed, paraffin embedded liver sections. During the review of the slides we noticed that neutrophils were also positive in addition to proliferating hepatocytes using DAB as the chromagen. When I switched to Vector VIP the neutrophils were negative. Has anyone else observed neutrophil labeling with Ki-67 using DAB? Could the DAB be reacting with the neutrophils? David L. Krull Scientist, Molecular & Ultrastructural Pathology GSK Safety Assessment 9.2103 5 Moore Drive RTP, NC 27709-3398 Phone: 919-315-2457 [email protected] _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet ...
Invitrogen Anti-Ki-67 Monoclonal (20Raj1), eBioscience™, Catalog # 69-5699-42. Tested in Flow Cytometry (Flow) applications. This antibody reacts with Canine, Human samples. Supplied as 100 Tests purified antibody (5 µL/Test).
Rabbit recombinant monoclonal Ki67 antibody [SP6] validated in Knockout cell lines, WB, IHC, ICC/IF and tested in Human, Mouse and Rat. Referenced in 83…
Plays a critical role in epithelial cell morphogenesis, polarity, adhesion and cytoskeletal organization in the lens (PubMed:26231217).
This is a set of high-quality photo-etched metal detail parts from Eduard, as shown here for the application described in the title. This item is a photo-etched detail parts set for use with aircraft kits.
आमचे सर्व लेख मिळवण्यासाठी फेसबूक, इंस्टाग्राम मनामध्ये जर काही इच्छा असतील कुणा विषयी काही करण्याची आवड…. ...
2002). "The Ki-67 protein interacts with members of the heterochromatin protein 1 (HP1) family: a potential role in the ... CBX3 has been shown to interact with PIM1, Ki-67, Lamin B receptor, CBX5 and CBX1. Heterochromatin protein 1 GRCh38: Ensembl ... 2003). "Interaction of the chromatin compaction-inducing domain (LR domain) of Ki-67 antigen with HP1 proteins". Genes Cells. 7 ... of Ki-67 antigen with HP1 proteins". Genes Cells. 7 (12): 1231-42. doi:10.1046/j.1365-2443.2002.00596.x. ISSN 1356-9597. PMID ...
Li H, Byeon IJ, Ju Y, Tsai MD (2004). "Structure of human Ki67 FHA domain and its binding to a phosphoprotein fragment from ... There are two conserved sequence motifs within the FHA Ki67 binding domain: TPVCTP and LERRKS, this domain binds to the ... interacts with the forkhead associated domain of Ki-67 antigen in mitosis". J Biol Chem. 276 (27): 25386-91. doi:10.1074/jbc. ... near to the N-terminus and a FHA Ki67 binding domain near to the C-terminus. ...
Prostate specific membrane antigen is a transmembrane carboxypeptidase and exhibits folate hydrolase activity.[75] This protein ... The expression of Ki-67 by immunohistochemistry may be a significant predictor of patient outcome for men with prostate cancer. ... Prostate cancer screening is controversial.[1][3] Prostate-specific antigen (PSA) testing increases cancer detection but does ... Although the widespread use of prostate-specific antigen (PSA) screening in the US has resulted in diagnosis at earlier age and ...
It was shown that two proliferation markers, Ki-67 and proliferating cell nuclear antigen (PCNA), are regulated by these ...
Prostate specific membrane antigen is a transmembrane carboxypeptidase and exhibits folate hydrolase activity.[72] This protein ... The expression of Ki-67 by immunohistochemistry may be a significant predictor of patient outcome for men with prostate cancer. ... Options include the digital rectal exam (DRE) and the prostate-specific antigen (PSA) blood test.[90] Such screening is ... Prostate cancer screening is controversial.[3][10] Prostate-specific antigen (PSA) testing increases cancer detection, but it ...
"Assessment of Ki67 in Breast Cancer: Recommendations from the International Ki67 in Breast Cancer Working Group". JNCI Journal ... Similarly, proliferating cell nuclear antigen (PCNA) is a protein associated with cell proliferation that is upregulated in ... Ki-67 is a nuclear antigen expressed in proliferating cells that is coded by the MKI67 gene on chromosome 10, and is expressed ... The Ki-67 score closely correlates with other proliferation markers, and has been shown to have prognostic and predictive value ...
... tissue polypeptide antigen MeSH D23.050.290.249 - epstein-barr virus nuclear antigens MeSH D23.050.290.500 - ki-67 antigen MeSH ... antigens, cd15 MeSH D23.101.100.900.131 - antigens, cd31 MeSH D23.101.100.920 - antigens, ly MeSH D23.101.100.930 - antigens, ... forssman antigen MeSH D23.050.285.018 - antigens, cd24 MeSH D23.050.285.025 - antigens, cd30 MeSH D23.050.285.040 - antigens, ... hla-a antigens MeSH D23.050.301.500.450.370.372 - hla-a1 antigen MeSH D23.050.301.500.450.370.374 - hla-a2 antigen MeSH D23.050 ...
Antibodies against proliferating cell nuclear antigen (PCNA) or monoclonal antibody termed Ki-67 can be used for grading of ... Proliferating cell nuclear antigen (PCNA) is a DNA clamp that acts as a processivity factor for DNA polymerase δ in eukaryotic ... PCNA was originally identified as an antigen that is expressed in the nuclei of cells during the DNA synthesis phase of the ... "Entrez Gene: PCNA proliferating cell nuclear antigen". Leonardi E, Girlando S, Serio G, Mauri FA, Perrone G, Scampini S, Dalla ...
Antigen KI-67 also known as Ki-67 or MKI67 (Marker Of Proliferation Ki-67) is a protein that in humans is encoded by the MKI67 ... Inactivation of antigen KI-67 leads to inhibition of ribosomal RNA synthesis. The Ki-67 protein (also known as MKI67) is a ... "Entrez Gene: Antigen identified by monoclonal antibody Ki-67". Schonk DM, Kuijpers HJ, van Drunen E, van Dalen CH, Geurts van ... Antigen KI-67 is a nuclear protein that is associated with cellular proliferation. Altering Ki-67 expression levels did not ...
They are negative for androgen receptor and prostate-specific antigen (PSA) and are positive for prostate acid phosphatase. ... Neuroendocrine tumor cells are also negative for the proliferation marker Ki-67; however, adjacent non-neuroendocrine tumor ... androgen receptor and prostate-specific antigen. It is commonly believed that prostatic adenocarcinoma exhibiting significant ... cells appear to display an increased expression of Ki-67. As opposed to their normal neuroendocrine counterparts, tumor ...
Bunce M, Barnardo MC, Procter J, Marsh SG, Vilches C, Welsh KI (December 1996). "High resolution HLA-C typing by PCR-SSP: ... February 2004). "Human leukocyte antigen-A, -B, and -Cw polymorphism in a Berber population from North Morocco using sequence- ... March 2000). "HLA genes in Arabic-speaking Moroccans: close relatedness to Berbers and Iberians". Tissue Antigens. 55 (3): 239- ... Tissue Antigens. 50 (6): 593-601. doi:10.1111/j.1399-0039.1997.tb02917.x. PMID 9458112. Torimiro JN, Carr JK, Wolfe ND, et al ...
Prostate-specific antigen-based prostate cancer screening: Past and future»։ International Journal of Urology 22 (6): 524-32։ ... Ki-67- ի էքսպրեսիան իմունոհիստոքիմիայի միջոցով կարող է զգալիորեն կանխատեսել շագանակագեղձի քաղցկեղի ելքը[80]։ ... Prostate cancer screening with prostate-specific antigen: A guide to the guidelines»։ Prostate International 4 (4): 125-29։ ... Expression of prostate-specific membrane antigen (PSMA), increases cell folate uptake and proliferation and suggests a novel ...
Similarly, Ki-67 was seen less in the central MEC compared to the other lesions, though this discovery is not essential to the ... Proliferating cell nuclear antigen readings were established to have no role in the differentiation process. The TGF-beta ... Past studies observed Ki-67, p53, and PCNA expression in common jaw cysts that shared similar characteristics. There was a lack ...
SGc tumor cells stain positive with epithelial membrane antigen (EMA), cytokeratin -7 (CK-7), Ber-EP4, adipophilin, perilipin, ... Ki-67 positivity, and PD-1 expression. Poorly or undifferentiated tumors are more likely to have nodal involvement and are ... and androgen receptor (AR). Meanwhile, SGc cells are negative for carcinoembryonic antigen (CEA), gross cystic disease fluid ... The mean age of diagnosis for periocular and extraocular SGc is around 67 years. Periocular SGc tends to be more common in ...
Imai Y, Soda M, Hatakeyama S, Akagi T, Hashikawa T, Nakayama KI, Takahashi R (Jul 2002). "CHIP is associated with Parkin, a ... "Identification of tumor-associated antigens in chronic lymphocytic leukemia by SEREX". Blood. 100 (6): 2123-31. doi:10.1182/ ... Imai Y, Soda M, Hatakeyama S, Akagi T, Hashikawa T, Nakayama KI, Takahashi R (Jul 2002). "CHIP is associated with Parkin, a ... "Characterization of human colon cancer antigens recognized by autologous antibodies". International Journal of Cancer. 76 (5): ...
"MACROPHAGE ANTIGEN CD68; CD68". omim.org. Retrieved 16 September 2017. Leong, Anthony S-Y; Cooper, Kumarason; Leong, F Joel W-M ... Monoclonals that react with humans include, Ki-M7, PG-M1, 514H12, ABM53F5, 3F7C6, 3F7D3, Y1/82A, EPR20545, CDLA68-1, LAMP4-824 ... Other names or aliases for this gene in humans and other animals include: CD68 Molecule, CD68 Antigen, GP110, Macrosialin, ... and Ki-67 proliferative index with clinical correlation". Archives of Pathology & Laboratory Medicine. 128 (7): 771-5. doi: ...
... the nonspecific cross-reacting antigen of carcinoembryonic antigen". Cancer Res. 57 (24): 5460-5464. PMID 9407950. Kawaharata H ... The expression of H19 in these cells is also independent of the tumor suppressor protein p53 and the cell cycle marker Ki-67. ... p95, or NCA-90, is related to carcinoembryonic antigens, which have been found to reduce drug toxicity by Kawaharata et al. NCI ... Hinoda Y, Itoh F, Endo T, Oikawa S, Nakazato H, Imai K (July 1997). "Decreased sensitivity of carcinoembryonic antigen cDNA- ...
The same name is also used to refer to the gene which codes for the antigen. The MART-1/melan-A antigen is specific for the ... 2005). "Expression of Melan-A and Ki-67 in desmoplastic melanoma and desmoplastic nevi". The American Journal of ... of Belgium called the gene melan-A, presumably an abbreviation for "melanocyte antigen." MART-1/melan-A is a protein antigen ... Protein melan-A also known as melanoma antigen recognized by T cells 1 or MART-1 is a protein that in humans is encoded by the ...
... and CD79A and have a very low proliferation rate as defined by the intensity of their expression of Ki-67. They also ... develops as a consequence of chronic inflammation and specific antigen stimulation. In support of this possibility, there have ... and associations with chronic inflammatory diseases and chronic antigen stimulation; Mantle cell lymphoma differs from DFL by ...
"Strong association between IgA nephropathy and HLA-DR4 antigen". Kidney Int. 22 (4): 377-82. doi:10.1038/ki.1982.185. PMID ... Kaibe M, Takakuwa K, Murakawa H, Ishii K, Tamura M, Tanaka K (2006). "Studies on the human leukocyte antigens in patients with ... Matsuyama M, Hashimoto K, Yamasaki Y, Shirakura R, Higuchi R, Miyajima T, Amemiya H (1981). "HLA-DR antigens in pemphigus among ... Tissue Antigens. 41 (2): 57-64. doi:10.1111/j.1399-0039.1993.tb01980.x. PMID 8475491. Undlien D, Friede T, Rammensee H, Joner G ...
The highest number of patients identified was obtained by combination of thymidine kinase and Ki-67 staining. The technique has ... February 2012). "Exposed proliferation antigen 210 (XPA-210) in renal cell carcinoma (RCC) and oncocytoma: clinical utility and ... carcinoembryonic antigen) and AFP (alpha fetoprotein). The genes for these tumor markers may be used as promoter genes for ... cytosolic thymidine kinase as compared to proliferating cell nuclear antigen in patients with colorectal carcinoma". Anticancer ...
2016) Woroniecka KI, Chongsathidkiet P, Rhodin KE, Kemeny HR, Dechant CA, Farber SH, Elsamadicy AA, Cui X, Koyama S, Jackson CC ... Rescuing imperfect antigens for immune-oncology. Nat Biotech. Epub ahead of print. Aug 2019. "Peter Edward Fecci". "Leadership ... 13 (7): 2158-67. doi:10.1158/1078-0432.CCR-06-2070. PMID 17404100. "Regulatory T cells move in when gliomas say "I-DO"". PMC ... 13 (7): 2158-67. doi:10.1158/1078-0432.CCR-06-2070. PMID 17404100. "Peter Edward Fecci". "Fecci Appointed Associate Deputy ...
Notably, the afflicted T cells have an extremely low rate of proliferation as determined by examining their KI-67 protein using ... Unlike celiac disease, ITCLD-GT: a) is unresponsive to gluten-free diets: b) is negative for antigens detected in standard ... has infiltrating clonal T cells which do not express HLA-DQ2 or HLA-DQ8 human leukocyte antigens; and d) have lesions populated ... fusion between interleukin-2 and B-cell maturation antigen genes, loses in the 4p26 and 16p13 chromosomal areas involved in ...
Ki Choi, Young; Goyal, Sagar M.; Soo Joo, Han (2007). "Serologic and genetic characterization of North American H3N2 swine ... like virus a B/Brisbane 60/2008-like antigens A separate vaccine was available for pandemic H1N1 influenza using the A/ ... like virus a B/Brisbane/60/2008-like antigens The vaccines produced for the 2011-2012 season used: an A/California/07/2009 ( ... A/Wisconsin/67/2005 (H3N2) and A/Hiroshima/52/2005 were used at the time) a B/Malaysia/2506/2004-like virus "A/H3N2 has become ...
Arnemann J, Spurr NK, Buxton RS (May 1992). "The human gene (DSG3) coding for the pemphigus vulgaris antigen is, like the genes ... Czerwenka KF, Manavi M, Hosmann J, Jelincic D, Pischinger KI, Battistutti WB, Behnam M, Kubista E (2001). "Comparative analysis ... Kárpáti S, Amagai M, Prussick R, Cehrs K, Stanley JR (July 1993). "Pemphigus vulgaris antigen, a desmoglein type of cadherin, ... Desmoglein List of target antigens in pemphigus List of conditions caused by problems with junctional proteins GRCh38: Ensembl ...
They are rapidly proliferating cells as determined by testing for Ki-67 staining. In at least seventy-five percent of cases, ... Immune privileged sites are sanctuaries where abnormal antigens that occur in them, such as those that are expressed on cancer ... 52 (1): 53-67. doi:10.1016/j.pathol.2019.08.013. PMID 31735345. Twa DD, Mottok A, Savage KJ, Steidl C (May 2018). "The ...
They are often found within tissues where they can be activated by antigen-presenting cells upon infection. They are present in ... Akhtar M, al-Dayel F, Siegrist K, Ezzat A (1996). "Neutrophil-rich Ki-1-positive anaplastic large cell lymphoma presenting as a ... Pöllänen P, Maddocks S (1988). "Macrophages, lymphocytes and MHC II antigen in the ram and the rat testis". Journal of ... Macrophages are directly involved in the fight against invading micro-organisms as well as being antigen-presenting cells which ...
... analysis of their Ki-67 protein levels), lack evidence of Epstein-Barr virus infection, gene mutations, or chromosome ... and T-cell intracellular antigen-1 cytotoxic proteins within their cytoplasmic granules. This pattern of protein expression ...
Greiner, Hansel; Leach, James L.; Lee, Ki-Hyeong; Krueger, Darcy A. (April 2011). "Anti-NMDA receptor encephalitis presenting ... The recruitment of the complement cascade via the classical pathway (antibody-antigen interaction). Membrane attack complex ( ... 133 (6): 1655-67. doi:10.1093/brain/awq113. PMC 2877907. PMID 20511282. Suh-Lailam BB, Haven TR, Copple SS, Knapp D, Jaskowski ... 67 (1): 141-142. doi:10.1002/ana.21928. ISSN 0364-5134. PMID 20186949. S2CID 2366741. "A YoungReporter Chronicles Her 'Brain On ...
OspA antigens, shed by live Borrelia bacteria into urine, are a promising technique being studied.[117] The use of nanotrap ... Swanson KI, Norris DE (2007). "Detection of Borrelia burgdorferi DNA in lizards from Southern Maryland". Vector Borne and ... The CDC does not recommend urine antigen tests, PCR tests on urine, immunofluorescent staining for cell-wall-deficient forms of ... burgdorferi sensu stricto antigens in people have been identified in Colombia,[237] and Bolivia.[citation needed] ...
a B/Brisbane 60/2008-like antigens[23]. A separate vaccine was available for pandemic H1N1 influenza using the A/California/7/ ... René Gramer, Marie; Hoon Lee, Jee; Ki Choi, Young; Goyal, Sagar M.; Soo Joo, Han (2007). "Serologic and genetic ... an A/Wisconsin/67/2005 (H3N2)-like virus (A/Wisconsin/67/2005 (H3N2) and A/Hiroshima/52/2005 were used at the time) ... an A/Wisconsin/67/2005 (H3N2)-like virus (A/Wisconsin/67/2005 and A/Hiroshima/52/2005 strains) ...
HLA-DQ is part of the MHC class II antigen-presenting receptor (also called the human leukocyte antigen) system and ... Morley KI, Mulder CJ, Murray JA, Núñez C, Oosterom E, Ophoff RA, Polanco I, Peltonen L, Platteel M, Rybak A, Salomaa V, ... Modern anti-tTG assays rely on a human recombinant protein as an antigen.[89] tTG testing should be done first as it is an ... Tissue Antigens. 47 (2): 127-33. doi:10.1111/j.1399-0039.1996.tb02525.x. PMID 8851726.. ...
Mells GF, Floyd JA, Morley KI, et al. (April 2011). "Genome-Wide Association study Identifies 12 New Susceptibility Loci for ... March 2006). "Increased expression of Nuclear Envelope gp210 Antigen in Small Bile Ducts in Primary Biliary Cirrhosis". Journal ... 67] It has supported the initiative for a change in name.[9] ...
Griffith GL, Maull KI, Sachatello CR.1977.Septic pulmonary embolization.Surg Gynecol Obstet.1977 Jan;144(1):105-8.PMID: 318771 ... വൈറസുകളുടെ കോശസ്തരത്തിൽ നിന്ന് പൊഴിയുന്ന പ്രതിജനകങ്ങളെ (antigen) രോഗിയുടെ രക്തത്തിൽ അളന്നെടുക്കുന്നതാണ്‌ മറ്റൊരു രീതി. വൈറസുകൾ ... Jeong YJ, Kim KI, Seo IJ, et al.Eosinophilic lung diseases: a clinical, radiologic, and pathologic overview. Radiographics. ... antigen testing) ചെയ്യുകയും ആവാം.ആവശ്യമെങ്കിൽ ശ്വാസനാളിയിൽ ക്യാമറഘടിപ്പിച്ച കുഴൽ കടത്തി ചെയ്യുന്ന എൻഡോസ്കോപ്പി പരിശോധനയും ഫലം ...
Wang H, Bauzon F, Ji P, Xu X, Sun D, Locker J, Sellers RS, Nakayama K, Nakayama KI, Cobrinik D, Zhu L (January 2010). "Skp2 is ... and a molecule called proliferating cell nuclear antigen, or PCNA, which speeds DNA replication and repair by helping to attach ... 67 (12): 7641-7. PMC 238233. PMID 8230483.. *^ Wu CL, Zukerberg LR, Ngwu C, Harlow E, Lees JA (May 1995). "In vivo association ...
However, this antigen disappears rapidly ex vivo. Recently, however, the surface antigen CD319 (SLAMF7) was discovered to be ... doi:10.1038/ki.2012.414. PMID 23302715.. *^ Castillo JJ (2016). "Plasma Cell Disorders". Primary Care. 43 (4): 677-691. doi: ... belantamab mafodotin - a monoclonal antibody against B-cell maturation antigen (BCMA), also known as CD269, indicated for the ... 67. Switzerland: S. Karger AG. p. 105. ISBN 978-3-8055-6460-1. . Archived from the original on 2016-05-27.. ...
Kompleks antigen-PHK prepoznajo tudi koreceptorji CD4, ki novačijo molekule iz T-celice (npr. Lck tirozinsko kinazo), ki so ... Funkcije celic pomagalk: antigen predstavitvene celice (APC) predstavljajo antigene na MHC II molekulah, ki jih pomagalke tudi ... Delujejo tudi kot lovilci, ki izločajo izčrpane celice in druge ostanke iz telesa. Poleg tega so tudi antigen prestavitvene ... Po drugi strani so za antigen specifični receptorji na površini B-celic molekule protiteles, ki prepoznajo celotne patogene ...
doi:10.1038/ki.2015.178. PMID 26108068.. *^ Mreich E, Chen XM, Zaky A, Pollock CA, Saad S (June 2015). "The role of Krüppel- ... "Transcriptional regulation of A33 antigen expression by gut-enriched Krüppel-like factor". Oncogene. 22 (28): 4434-43. doi ...
Hahn JY, Cho HJ, Bae JW, Yuk HS, Kim KI, Park KW, Koo BK, Chae IH, Shin CS, Oh BH, Choi YS, Park YB, Kim HS (Oct 2006). "Beta- ... Ren J, Li Y, Kufe D (May 2002). "Protein kinase C delta regulates function of the DF3/MUC1 carcinoma antigen in beta-catenin ... Yamamoto M, Bharti A, Li Y, Kufe D (May 1997). "Interaction of the DF3/MUC1 breast carcinoma-associated antigen and beta- ... "The c-Src tyrosine kinase regulates signaling of the human DF3/MUC1 carcinoma-associated antigen with GSK3 beta and beta- ...
"Megszavazta az Országgyűlés a koronavírus-törvényt, Áder pedig ki is hirdette". 30 March 2020. "Áder János már alá is írta a ... were tested positive for COVID-19 from a rapid antigen test after being exposed to people who were not wearing a mask while ... 67-85, doi:10.3998/mpub.11927713.6, ISBN 978-0-472-90246-0, JSTOR 10.3998/mpub.11927713.6 "China is winning the coronavirus ...
doi:10.1038/sj.ki.5002514. PMID 17805240. Vanhoutte D, Schellings MW, Götte M, Swinnen M, Herias V, Wild MK, et al. (January ... which is linked to increased resistance to chemotherapy and radiation therapy It is a specific antigen on multiple myeloma ... 153 (2): 456-67. doi:10.1006/cimm.1994.1042. PMID 8118875. Maruyama K, Sugano S (January 1994). "Oligo-capping: a simple method ...
Izbicki G, Chavko R, Banauch GI, Weiden MD, Berger KI, Aldrich TK, Hall C, Kelly KJ, Prezant DJ (May 2007). "World Trade Center ... Grunewald J, Eklund A, Olerup O (March 2004). "Human leukocyte antigen class I alleles and the disease course in sarcoidosis ... their immune system rebounds and the result is that it starts to attack the antigens of opportunistic infections caught prior ... 67 (3): 263-76. doi:10.1016/j.crad.2011.04.018. PMID 22094184. Tavee J, Culver D (June 2011). "Sarcoidosis and small-fiber ...
doi:10.1038/ki.2012.414. PMID 23302715. Elsaman AM, Radwan AR, Akmatov MK, Della Beffa C, Walker A, Mayer CT, Dai L, Nativ S, ... processing of these antigens. As they are stimulated to become plasma cells, B cells refashion parts of their genome in efforts ... and the immunoglobulin light chain antigen binding locus on the q arm of chromosome 22 at position 11.2 (i.e. 22 q11.2) by ... and recombine various genes at the immunoglobulin heavy chain antigen-binding locus on the long (i.e. "q") arm of human ...
Istimonius ki furunt a ssegari su saltu de pari (et) a poniri sus treminis, donnu Cumita de Lacun, ki fut curatori de Canpitanu ... Arnaiz-Villena A, Rodriguez de Córdoba S, Vela F, Pascual JC, Cerveró J, Bootello A. - HLA antigens in a sample of the Spanish ... Et apirus cummentu in su campaniu, ki fegir(us), d'arari issus sas terras ipsoru ki sunt in su saltu miu et (ll)u castiari s(u ... fazzu mi carta pro kertu ki fegi cun isus de Maara pro su saltu ubi si ( ... )ari zizimi ( ... ) Maara, ki est de sanctu ...
"Entrez Gene: ALK anaplastic lymphoma kinase (Ki-1)". Shiota M, Fujimoto J, Semba T, Satoh H, Yamamoto T, Mori S (June 1994). " ... March 2002). "ALK as a novel lymphoma-associated tumor antigen: identification of 2 HLA-A2.1-restricted CD8+ T-cell epitopes". ... Yaakup H, Sagap I, Fadilah SA (October 2008). "Primary oesophageal Ki (CD30)-positive ALK+ anaplastic large cell lymphoma of T- ... "Hyperphosphorylation of a novel 80 kDa protein-tyrosine kinase similar to Ltk in a human Ki-1 lymphoma cell line, AMS3". ...
Relationship between AgNOR Proteins, Ki-67 Antigen, p53 Immunophenotype and Differentiation Markers in Archival Breast ...
K67LI is defined as Ki-67 antigen-labeling index rarely. ... How is Ki-67 antigen-labeling index abbreviated? K67LI stands ... Serum SCC antigen may be a useful biologic marker in patients with sinonasal IP [205-207]. Proliferating cell nuclear antigen ... n.d.) Acronym Attic. (2019). Retrieved August 24 2019 from https://www.acronymattic.com/Ki_67-antigen_labeling-index-(K67LI). ... S.v. "K67LI." Retrieved August 24 2019 from https://www.acronymattic.com/Ki_67-antigen_labeling-index-(K67LI).html ...
One such antibody, MIB1, recognizes a fixation and embedding resistant epitope on the Ki-67 protein if sections are previously ... Novel antibodies have been generated by immunizing with bacterially expressed fragments of the repetitive motif of the Ki-67 ... Detection of the Ki-67 antigen in fixed and wax-embedded sections with the monoclonal antibody MIB1 Histopathology. 1993 Apr;22 ... Thus, MIB1 represents a new anti-Ki-67 antibody which appears to be a robust marker of cell proliferation easily applicable to ...
... and Ki67 proliferation-associated antigen increased during the progression of human colon, gastric, and breast carcinomas. ... Augmentation of type IV collagenase, laminin receptor, and Ki67 proliferation antigen associated with human colon, gastric, and ... and Ki67 proliferation-associated antigen increased during the progression of human colon, gastric, and breast carcinomas. ... A positive correlation (P less than 0.001) was found for the immunoreactivity of all three antigens in the invasive carcinomas ...
Chmadrin: a novel Ki-67 antigen-related perichromosomal protein possibly implicated in higher order chromatin structure ... Chmadrin: a novel Ki-67 antigen-related perichromosomal protein possibly implicated in higher order chromatin structure ... Chmadrin: a novel Ki-67 antigen-related perichromosomal protein possibly implicated in higher order chromatin structure ... Chmadrin: a novel Ki-67 antigen-related perichromosomal protein possibly implicated in higher order chromatin structure ...
The antibodies MIB 1 and MIB 3 required the five amino acid sequence FKELF for binding, whereas Ki-67, JG-67-2a, MIB 5 and IND. ... CONCLUSIONS--It is concluded that the amino acid sequence FKELF represents an immunodominant area of the Ki-67 protein and that ... analysis of antibodies recognising the cell proliferation associated nuclear antigen previously defined by the antibody Ki-67 ( ... analysis of antibodies recognising the cell proliferation associated nuclear antigen previously defined by the antibody Ki-67 ( ...
Ki-67 antigen overexpression is associated with the metaplasia-adenocarcinoma sequence in Barretts esophagus. ... The Ki-67 index averaged 10 ± 4% in patients with normal gastric mucosa (n = 17), 21 ± 15% in patients with Barretts esophagus ... Ki-67 antigen has increased expression along the metaplasiaadenocarcinoma sequence. There is a strong linear correlation ... The objective of this study was to evaluate Ki-67 antigen expression in patients with Barretts esophagus and esophageal ...
Antigen Identified by Monoclonal Antibody Ki-67, KIA, MKI67, Proliferation Related Ki-67, item number: K1700-11A.100 from ... Ki67 antigen is the prototypic cell cycle related nuclear protein, expressed by proliferating cells in all phases of the active ... Ki67 antigen is the prototypic cell cycle related nuclear protein, expressed by proliferating... more ... The correlation between low Ki67 index and histologically low-grade tumors is strong. Ki67 is routinely used as a neuronal ...
The antigen defined by mAb Ki-67 is a human nuclear protein the expression of which is strictly associated with cell ... The cell proliferation-associated antigen of antibody Ki-67: a very large, ubiquitous nuclear protein with numerous repeated ... The cell proliferation-associated antigen of antibody Ki-67: a very large, ubiquitous nuclear protein with numerous repeated ... Ki-67 detects a double band with apparent molecular weights of 395 and 345 kD in immunoblots of proteins from proliferating ...
... antibody/antibodies for many antigens and hosts, IgA, IgD, IgE, IgG, IgM. Antibody application for WB, ELISA, IHC-P, IF, FCM. ... You need info about Mouse Antigen KI-67 (MKI67) ELISA Kit or any other Gentaur produtct? Contact us on Live Chat Our specialist ...
Who would have thought a single Ki67 measurement would predict long-term outcome?  Dowsett, M; AHern, R; Salter, J; Zabaglo, ... Development of a Ki-67-based clinical trial assay for neoadjuvant endocrine therapy response monitoring in breast cancer.  ... Prognostic value of automated KI67 scoring in breast cancer: a centralised evaluation of 8088 patients from 10 study groups.  ... Ki67 Is an Independent Predictor of Recurrence in the Largest Randomized Trial of 3 Radiation Fractionation Schedules in ...
4.3 Surface antigen/KI-67 (Phenotype of Proliferating Cells). Reagents. *3.54 g Na2HPO4 in 100 mL DDW (buffer is 0.2M) ... 4. Proliferation Antigens. 4.1 PCNA (Proliferating Cell Nuclear Antigen). Reagents and Antibodies. *PBS ... Add 10 µL KI-67 FITC diluted with 40 µL PBS/BSA (1%). Prepare isotypic control. Incubate for 30 minutes on ice. Wash twice with ... 2.2 Two surface antigens/DNA (counterstaining with DAPI). *2 x 106 cells are stained with two monoclonal antibodies labeled ...
Antigen KI-67 also known as Ki-67 or MKI67 is a protein that in humans is encoded by the MKI67 gene (antigen identified by ... Inactivation of antigen KI-67 leads to inhibition of ribosomal RNA synthesis. Ki-67 is an excellent marker to determine the ... The antigen KI-67 is a nuclear protein that is associated with and may be necessary for cellular proliferation. ... KI 67 Antigen immunohistochemistry in intracranial meningioma among sudanese patients. Author: Alsadig Gassoum, Mohamed A. ...
Ki-67 antigen; RRID:AB_2109815; RRID:AB_2216104; RRID:AB_2532342; RRID:AB_442102; RRID:AB_514500; RRID:AB_840257; RRID:SCR_ ... a) Ki-67 immunofluorescence demonstrates a highly proliferative zone in the α1 tanycyte domain (boxed area). (b1-4) Higher ... c) The number of Ki-67 cells shows large variations among 6 male and 6 female rats in tanycytic domains, the median eminence ( ... Ki-67 in Prss56-expressing cells (white arrows), including an α1 tanycyte (d, e), parenchymal cells (f, g) and β1 tanycytes (f ...
Ki-67 Rat anti-Canine, Cynomolgus Monkey, Human, Mouse, Non-human primate, Rat, eFluor(T) 506, Clone: SolA15, eBioscience 100 ... antigen identified by monoclonal antibody Ki-67; antigen KI-67; KIA; proliferation-related Ki-67 antigen; protein phosphatase 1 ... Ki-67 is expressed in all cell types and is detectable during active phases of the cell cycle (G1, S, G2, and mitosis) but is ... Ki-67 is a nuclear protein that is expressed during various stages in the cell cycle, particularly during late G1, S, G2, and M ...
mRNA levels of Ki67 and PCNA were determined by real-time PCR. (d) Protein levels of PCNA were determined by Western blot. Data ... S. E. Johnson and R. E. Allen, "Proliferating cell nuclear antigen (PCNA) is expressed in activated rat skeletal muscle ... The results were shown in Figures 2(b) and 2(c); mRNA levels of Ki-67 and PCNA were obviously upregulated by treatment with 2-8 ... Effect of γ-tocotrienol on cell cycle progression, relative mRNA, and protein levels of Ki67 and PCNA in MC3T3-E1 cells. (a) ...
PROLIFERATION INDICES OF VESTIBULAR SCHWANNOMAS BY Ki-67 AND PROLIFERATING CELL NUCLEAR ANTIGEN. Szeremeta, Wasyl; Monsell, ...
Both HPV-positive tumors had a high Ki-67 labeling index. Two of the four cases with a good response to therapy were hrHPV- ... Both HPV-positive tumors had a high Ki-67 labeling index. Two of the four cases with a good response to therapy were hrHPV- ... Both HPV-positive tumors had a high Ki-67 labeling index. Two of the four cases with a good response to therapy were hrHPV- ... Immunohistochemical staining was performed for Ki-67, p16(INK4A), and p53 expression. All cases were negative for the low-risk ...
... and Proliferating Cell Nuclear Antigen Expression in C Clinical Implication of p16, Ki-67, and Proliferating Cell Nuclear ... Ki-67 immunostaining did not increase the accuracy of HSIL diagnosis when combined with p16 immunostaining compared with p16 ... The primary objective was to evaluate clinical utility of Ki-67 and PCNA in combination with p16 in diagnosing HSIL. Also, we ... Subgroup analysis for conization specimen with CIN 2 and CIN 3 showed that positive Ki-67 immunostaining was an independent ...
Low Power Laser Irradiation Stimulates Cell Proliferation via Proliferating Cell Nuclear Antigen and Ki-67 Expression during ... Low Power Laser Irradiation Stimulates Cell Proliferation via Proliferating Cell Nuclear Antigen and Ki-67 Expression during ... The percentages of Ki-67 or PCNA positive cells were determined by counting positive nuclei (Ki-67/PCNA) and total nuclei in ... Animal wounds treated with single exposure of the 2 Jcm-2 indicated significant elevation in PCNA (P,0.01) and Ki-67 (P,0.05 ...
Fingerprint Dive into the research topics of Immunohistochemical status of the p53 protein and Ki-67 antigen using biopsied ... title = "Immunohistochemical status of the p53 protein and Ki-67 antigen using biopsied specimens can predict a sensitivity to ... T1 - Immunohistochemical status of the p53 protein and Ki-67 antigen using biopsied specimens can predict a sensitivity to ... Immunohistochemical status of the p53 protein and Ki-67 antigen using biopsied specimens can predict a sensitivity to ...
Low power laser irradiation stimulates cell proliferation via proliferating cell nuclear antigen and Ki-67 expression during ... Low power laser irradiation stimulates cell proliferation via proliferating cell nuclear antigen and Ki-67 expression during ...
MIB-1 identifies the Ki67 antigen present in nuclei of cells in all phases of the cell cycle except G0. Methods: We ... MIB-1 identifies the Ki67 antigen present in nuclei of cells in all phases of the cell cycle except G0. Methods: We ... MIB-1 identifies the Ki67 antigen present in nuclei of cells in all phases of the cell cycle except G0. Methods: We ... MIB-1 identifies the Ki67 antigen present in nuclei of cells in all phases of the cell cycle except G0. Methods: We ...
The purpose of this study was to evaluate the clinical utility of the Ki-67 MoAb for predicting survival. Using a prospective ... The purpose of this study was to evaluate the clinical utility of the Ki-67 MoAb for predicting survival. Using a prospective ... The purpose of this study was to evaluate the clinical utility of the Ki-67 MoAb for predicting survival. Using a prospective ... The purpose of this study was to evaluate the clinical utility of the Ki-67 MoAb for predicting survival. Using a prospective ...
Ki-67 proliferative index was 2.80% in the original tumor, 4.40% in the relapse, and 4.45% in the metastasis. The figure in the ... O índice proliferativo Ki-67 foi 2,80% no tumor original, 4,40% na recidiva e 4,45% na metástase. O valor obtido na recidiva é ... Amostras do tumor foram submetidas à análise imuno-histoquímica para os antígenos p53 e Ki-67. A coloração para p53 foi ... A high Ki-67 proliferative index in a pituitary adenoma might indicate a more aggressive behavior. ...
Antígeno Ki-67. Corticosteróides. Doenças da vulva. Líquen escleroso e atrófico. Marcadores biológicos. Proteína p53. ... "Líquen escleroso vulvar: estudo dos marcadores Ki-67 e p53 antes e após o tratamento com clobetasol a 0,05%, testosterona a 2% ... Vulvar lichen sclerosus: a study of markers Ki-67 and p53 before and after the treatment with clobetasol at 0.05%, testosterone ... Ki-67 Antigen. Lichen sclerosus et atrophicus. Protein p53. Testosterone. Vulvar diseases. ...
ACC, FAS, and Ki-67 were coexpressed with little SREBP in lung and cytotrophoblast. SREBP, ACC, and FAS were coexpressed ... Serial 5-microm sections were stained with antibodies to ACC, FAS, SREBP, and Ki-67 and were compared. ACC, FAS, SREBP, and Ki- ... Ki-67 Antigen / metabolism*. Sterol Regulatory Element Binding Protein 1. Transcription Factors*. ... The expression patterns of ACC, FAS, SREBP, and Ki-67 in fetal tissues were compared to determine whether SREBP is likely to ...
Antigen Ki-67, which is widely used as a cell proliferation marker, controls heterochromatin organisation and gene expression. ... The cell proliferation antigen Ki-67 organises heterochromatin Michal Sobecki et al. ...
immunogen = recombinant peptide representing one antigenic site of Ki-67 cDNA sequence ...
antigen identified by monoclonal antibody Ki-67. −1.79318. Cell proliferation. ASPM. asp (abnormal spindle) homolog. −1.98094. ... sperm associated antigen 5. −1.72396. Mitotic sister chromatic segregation. PRC1. protein regulator of cytokinesis 1. −1.89456 ...
  • Proliferating cell nuclear antigen labeling index . (acronymattic.com)
  • Other markers of proliferation (e.g. proliferating cell nuclear antigen) have been shown to be expressed in DNA repair, thus we investigated expression of MIB1 immunoreactivity in situations of DNA repair in vivo--ultraviolet irradiated human skin. (nih.gov)
  • Although the use of p16INK4a (p16) staining has been proven to improve diagnostic accuracy for high-grade squamous intraepithelial lesion (HSIL), the clinical evidence for use of Ki-67 and proliferating cell nuclear antigen ( PCNA ) is insufficient to make an independent recommendation for use, alone or in combination. (bvsalud.org)
  • Subsequently, tissue regeneration potential of these laser doses under study were evaluated by monitoring proliferating cell nuclear antigen and Ki-67 following the laser treatment and comparing it with the un-illuminated controls. (manipal.edu)
  • PCNA - Proliferating Cell Nuclear Antigen, expressed during the DNA synthesis. (wikipedia.org)
  • You need info about Mouse Antigen KI-67 (MKI67) ELISA Kit or any other Gentaur produtct? (gentaurshop.com)
  • Antigen KI-67 also known as Ki-67 or MKI67 is a protein that in humans is encoded by the MKI67 gene (antigen identified by monoclonal antibody Ki-67). (journalcra.com)
  • There are currently no images for Ki-67/MKI67 Antibody (NB110-90592F). (novusbio.com)
  • MKI67 is the human homologue of the product recognized by the monoclonal antibody Ki67, which is used as a tumor proliferation marker. (atlasgeneticsoncology.org)
  • There is one related pseudogene of MKI67 on chromosome X, named MKI67P1 (marker of proliferation Ki-67 pseudogene 1) with Gene ID 100271918. (atlasgeneticsoncology.org)
  • The Ki-67 protein (also known as MKI67) is a cellular marker for proliferation, and can be used in immunohistochemistry. (wikipedia.org)
  • Thus, MIB1 represents a new anti-Ki-67 antibody which appears to be a robust marker of cell proliferation easily applicable to archival material. (nih.gov)
  • Immunocytochemistry was performed with anti-BrdUrd (Beckton Dickinson), anti-SHH (Santa Cruz Biotechnology), and anti-Ki-67 (DAKO), using FITC- or horseradish peroxidase (HRP)-conjugated secondary antibodies (Boehringer Mannheim) as described ( 7 ). (pnas.org)
  • No. 554656) and stained with PerCP-Cy™5.5 Mouse anti-Ki-67 antibody (Cat. (bdbiosciences.com)
  • Epitope analysis of antibodies recognising the cell proliferation associated nuclear antigen previously defined by the antibody Ki-67 (Ki-67 protein). (bmj.com)
  • The cell proliferation-associated antigen of antibody Ki-67: a very large, ubiquitous nuclear protein with numerous repeated elements, representing a new kind of cell cycle-maintaining proteins. (rupress.org)
  • The present study targeted 30 patients diagnosed Vulvar Lichen Sclerosus, divided in three groups, and who made topical use of clobetasol propionate at 0.05%, testosterone at 2% and a placebo, so as to assess the evolution of the symptoms and the behavior of the cells marked with antibody Ki-67 and protein p53, before and after the treatments. (usp.br)
  • The Ki-67 protein was originally defined by the prototype monoclonal antibody Ki-67, which was generated by immunizing mice with nuclei of the Hodgkin lymphoma cell line L428. (wikipedia.org)
  • Using immunohistochemistry we evaluated the Ki-67 index in patients with Barrett's esophagus, esophageal adenocarcinoma, and controls.We included patients with endoscopically visible columnar mucosa of the distal esophagus (whose biopsies revealed specialized intestinal-type metaplasia), patients with esophageal and esophagogastric tumors types I and II, and patients with histologically normal gastric mucosa (control). (ufrgs.br)
  • For immunohistochemistry, all meningioma tumors at Elhassan Medical Laboratory for histopathology and cytology during the above mentioned period were processed for ki 67 antigen. (journalcra.com)
  • This Ki67 antibody is useful for Immunohistochemistry-paraffin embedded sections, Immunocytochemistry/immunofluorescence. (novusbio.com)
  • Applications Tested: This 20Raj1 antibody has been tested by immunocytochemistry on formaldehyde-fixed and permeabilized HeLa cells, and immunohistochemistry on FFPE human skin using low pH antigen retrieval. (thermofisher.com)
  • The percentages of Ki-67 or PCNA positive cells were determined by counting positive nuclei (Ki-67/PCNA) and total nuclei in five random fields per tissue sections. (manipal.edu)
  • MIB-1 identifies the Ki67 antigen present in nuclei of cells in all phases of the cell cycle except G0. (elsevier.com)
  • The ratio was converted to a percent proliferating cells by taking the number of Ki-67 positive stained nuclei in a given field and dividing by the total number of nuclei in that field and multiplying by 100. (clinicaltrials.gov)
  • High Ki-67 expression in an invasive breast cancer, with cancer nuclei being stained (brown). (wikipedia.org)
  • There is tumor cell positivity in 70% of the cells: Ki-67 labelling index = 70% Counting positive versus negative nuclei with Ki-67 labeling, in this case in a neuroendocrine tumor of the small intestine. (wikipedia.org)
  • Dividing cells show strong Ki-67 staining in cell nuclei while all cells contain large amounts of tubulin, the major component of microtubules. (wikipedia.org)
  • The antigen KI-67 is a nuclear protein that is associated with and may be necessary for cellular proliferation. (journalcra.com)
  • Due to its association with cell divison process, Ki-67 is routinely used as cellular proliferation marker of solid tumors as well as certain hematological malignancies, and a correlation has been demonstrated between Ki-67 index and the histopathological grade of cancers. (novusbio.com)
  • Finally, the morphological and cell cycle distribution of MIB1 expression is identical to that of Ki-67. (nih.gov)
  • The objective of this study was to evaluate Ki-67 antigen expression in patients with Barrett's esophagus and esophageal adenocarcinoma and to assess its correlation with the metaplasia-esophageal adenocarcinoma progression. (ufrgs.br)
  • Ki-67 antigen has increased expression along the metaplasiaadenocarcinoma sequence. (ufrgs.br)
  • The antigen defined by mAb Ki-67 is a human nuclear protein the expression of which is strictly associated with cell proliferation and which is widely used in routine pathology as a "proliferation marker" to measure the growth fraction of cells in human tumors. (rupress.org)
  • During interphase, Ki-67 expression is localized to the nucleus but redistributes to the chromosomes during mitosis and has specifically been found to associate with heterochromatin-bound proteins such as chromobox protein homolog 3 (CBX3). (fishersci.com)
  • In studies of tumor cells, Ki-67 expression has been used as a marker for determining the fraction of proliferating cells within a given population of tumor cells. (fishersci.com)
  • Ki-67 is shown to have a cell cycle dependent topographical distribution with perinucleolar expression at G1, expression in the nuclear matrix at G2, and expression on the chromosomes during M phase. (fishersci.com)
  • Immunohistochemical staining was performed for Ki-67, p16(INK4A), and p53 expression. (rug.nl)
  • Correlation of the immunohistochemical expression of p53 protein and Ki-67 antigen were studied using biopsied specimens of advanced esophageal cancer. (elsevier.com)
  • Conclusions: We conclude that in our patient population, nuclear proliferation in pancreatic cancer, as determined by expression of Ki67 nuclear antigen, does not appear to correlate with survival and is not a useful prognostic marker. (elsevier.com)
  • Despite intuitive thoughts to the contrary, there is no correlation between cell cycle analysis as determined by flow cytometry and Ki67 expression in pancreas cancer. (elsevier.com)
  • Antigen Ki-67, which is widely used as a cell proliferation marker, controls heterochromatin organisation and gene expression. (elifesciences.org)
  • The expression patterns of ACC, FAS, SREBP, and Ki-67 in fetal tissues were compared to determine whether SREBP is likely to participate in the regulation of proliferation-associated fatty acid synthesis during fetal growth. (biomedsearch.com)
  • Its expression occurs specially during late G1, S, G2 and M phases of the cell cycle, while in cells undergoing G0 phase, Ki67 remains undetectable. (novusbio.com)
  • Ki67 undergoes phosphorylation/dephosphorylation during mitosis, is susceptible to proteases and its structure implies that its expression is regulated by proteolytic pathways. (novusbio.com)
  • Expression occurs preferentially during late G1, S, G2 and M phases of the cell cycle, while in cells in G0 phase the antigen cannot be detected (at protein level) (PubMed:6206131). (abcam.com)
  • Ki67 expression was significantly higher in high-grade DCIS than in hyperplasia or low-grade DCIS. (aacrjournals.org)
  • Ki-67 is required for cell proliferation and its expression is restricted to the phases G 1 , S, G 2 , and M of the cell cycle. (biolegend.com)
  • There was also a marked reduction in immunohistochemical expression of Ki-67, a marker of cell proliferation. (pnas.org)
  • Moreover, TRAF6 expression was positively correlated with Ki-67 but inversely correlated with miR-146b-5p expression. (portlandpress.com)
  • Two-color flow cytometric analysis of Ki-67 expression by proliferating MOLT-4 and noncycling human peripheral blood mononuclear cells. (bdbiosciences.com)
  • Two-color flow cytometric dot plots showing the correlated expression patterns of DAPI (DNA) staining versus Ki-67 were derived from gated events with the forward and side light-scatter characteristics of intact MOLT-4 cells (Left Panel) or PBMC (Right Panel). (bdbiosciences.com)
  • Altering Ki-67 expression levels did not significantly affect cell proliferation in vivo. (wikipedia.org)
  • Expression of p53 protein and Ki-67 antigen in phyllodes tumor of the breast. (semanticscholar.org)
  • The prognostic value and its relationship with clinicopathological characteristics and Ki-67 (a proliferation-associated antigen) expression were analyzed. (springer.com)
  • In addition, TIN was positively correlated with Ki-67 expression ( p = 0.008). (springer.com)
  • Relationship between Ki-67 Antigen Expression and Histological Degree of Malignancy in Squamous Cell Carcinoma of Tongue. (nii.ac.jp)
  • Lastly, we performed cell cycle array analysis of Cl-amidine treated and control MCF10DCIS cells, and found that the PADI inhibitor strongly affects the expression of several cell cycle genes implicated in tumor progression, including p21 , GADD45α , and Ki67 . (biomedcentral.com)
  • Ki-67 detects a double band with apparent molecular weights of 395 and 345 kD in immunoblots of proteins from proliferating cells. (rupress.org)
  • We conclude that the Ki-67 antigen defines a new category of cell cycle-associated nuclear nonhistone proteins. (rupress.org)
  • Higher molecular weight proteins like ki67 may be more difficult to detect in WB. (abcam.com)
  • The N-terminal portion of Ki-67 contains a forkhead associated (FHA)1 domain, which is involved in interaction with proteins such as Hklp2 and NIFK. (miltenyibiotec.com)
  • Two histologic grading systems (Patnaik and Kiupel), special staining of toluidine blue, and IHC for KIT and Ki67 proteins were applied in both studies. (scielo.br)
  • Overall survival was significantly reduced in these patients with a high Ki-67- associated proliferative index compared with those with a low proliferative index (P = .001). (elsevier.com)
  • Both HPV-positive tumors had a high Ki-67 labeling index. (rug.nl)
  • The recommended ELISA Kit will likely detect the antigen better in the approved sample types than the available alternatives. (antibodies-online.com)
  • The recommended ELISA Kit will likely detect the antigen in question with higher specificity in approved samples than the available alternatives. (antibodies-online.com)
  • Perform heat mediated antigen retrieval before commencing with IHC staining protocol. (abcam.com)
  • One of its primary advantages over the original Ki-67 antibody (and the reason why it has essentially supplanted the original antibody for clinical use) is that it can be used on formalin-fixed paraffin-embedded sections, after heat-mediated antigen retrieval (see next section below). (wikipedia.org)
  • The proportion of neoplastic cells immunocytochemically positive for type IV collagenase (IVase), laminin receptor (LR), and Ki67 proliferation-associated antigen increased during the progression of human colon, gastric, and breast carcinomas. (nih.gov)
  • METHODS--Different parts of the Ki-67 protein cDNA were expressed in Escherichia coli. (bmj.com)
  • New mAbs against a bacterially expressed part and a synthetic polypeptide deduced from the isolated cDNA react with the native Ki-67 antigen, thus providing a circle of evidence that we have cloned the authentic Ki-67 antigen cDNA. (rupress.org)
  • The central part of the Ki-67 antigen cDNA contains a large 6,845-bp exon with 16 tandemly repeated 366-bp elements, the "Ki-67 repeats", each including a highly conserved new motif of 66 bp, the "Ki-67 motif", which encodes for the epitope detected by Ki-67. (rupress.org)
  • Computer analysis of the nucleic acid and the deduced amino acid sequence of the Ki-67 antigen confirmed that the cDNA encodes for a nuclear and short-lived protein without any significant homology to known sequences. (rupress.org)
  • Novel antibodies have been generated by immunizing with bacterially expressed fragments of the repetitive motif of the Ki-67 gene. (nih.gov)
  • AIMS--To elucidate the fine specificities of the antibodies MIB 1 and MIB 3 and of additional monoclonal antibodies which also recognise the Ki-67 protein (MIB 5, IND.64, JG-67-2a). (bmj.com)
  • The antibodies MIB 1 and MIB 3 required the five amino acid sequence FKELF for binding, whereas Ki-67, JG-67-2a, MIB 5 and IND.64 detected the sequence FKEL. (bmj.com)
  • CONCLUSIONS--It is concluded that the amino acid sequence FKELF represents an immunodominant area of the Ki-67 protein and that there is no correlation between the ability to detect the Ki-67 protein in paraffin wax sections irradiated with microwaves and the epitopes recognised by the antibodies. (bmj.com)
  • Ki67 antibodies are useful in establishing the cell growing fraction in neoplasms. (biomol.com)
  • Serial 5-microm sections were stained with antibodies to ACC, FAS, SREBP, and Ki-67 and were compared. (biomedsearch.com)
  • Ki-67 and MIB-1 monoclonal antibodies are directed against different epitopes of the same proliferation-related antigen. (wikipedia.org)
  • Ki67 antibodies are useful in establishing the cell growing fraction in neoplasms (immunohistochemically quantified by determining the number of Ki67-positive cells among the total number of resting cells = Ki67 index). (genetex.com)
  • One such antibody, MIB1, recognizes a fixation and embedding resistant epitope on the Ki-67 protein if sections are previously microwaved in a citrate buffer. (nih.gov)
  • Both isoforms contain one forkhead-associated domain and 16 concatenated "Ki-67 repeats," each containing the epitope recognized by the mAb Ki-67. (biolegend.com)
  • Antigen retrieval: Boil tissue section in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at room temperature for 20 min. (abcam.com)
  • Alternative Antigen retrieval techniques? (ihcworld.com)
  • The primary objective was to evaluate clinical utility of Ki-67 and PCNA in combination with p16 in diagnosing HSIL. (bvsalud.org)
  • The expressions of p16, Ki-67, and PCNA were evaluated by immunohistochemical methods in 149 cervical tissues encompassing 17 negative lesion, 31 CIN 1, 25 CIN 2, 41 CIN 3, and 35 invasive squamous cell carcinoma . (bvsalud.org)
  • To understand the mechanisms by which charged particle irradiation induce damage to uterine tissues, markers of DNA double strand breaks (ƴH2AX), apoptosis (activated Caspase-3), oxidative damage for protein (NTY) and Lipid (4-HNE), cell proliferation (PCNA) and Ki-67 will be analyzed. (nsbri.org)
  • In a wide range of normal tissues the distribution and number of MIB1 immunoreactive cells matched that of cryostat sections stained with Ki-67. (nih.gov)
  • The percentage of positive cells increased significantly (P less than 0.001) for all three antigens comparing carcinomas with adenomas and Dukes' C stage compared with Dukes' A/B stage. (nih.gov)
  • Gastric carcinomas classified as well-differentiated advanced stage contained a significantly higher proportion of tumor cells positive for IVase (P less than 0.001), LR (P less than 0.001), and Ki67 (P less than 0.001) compared with well-differentiated superficial tumors. (nih.gov)
  • Gastric carcinomas classified as poorly differentiated superficial had a significantly higher proportion of cells positive for Ki67 (P less than 0.016), but not IVase (P less than 0.069) or LR (P less than 0.075), compared with poorly differentiated advanced tumors. (nih.gov)
  • Ki67 antigen is the prototypic cell cycle related nuclear protein, expressed by proliferating cells in all phases of the active cell cycle (G1, S, G2 and M phase). (biomol.com)
  • Ki-67 antigen-specific antisense oligonucleotides inhibit the proliferation of IM-9 cell line cells, indicating that the Ki-67 antigen may be an absolute requirement for maintaining cell proliferation. (rupress.org)
  • Ki-67 is an excellent marker to determine the growth fraction of a given cell population, the fraction of Ki-67-positive tumor cells (the Ki-67 labeling index) is often correlated with the clinical course of cancer. (journalcra.com)
  • Two isoforms of Ki-67 exist, a 345 and 395 kDa form that are expressed in dividing cells. (fishersci.com)
  • Ki-67 is expressed in all cell types and is detectable during active phases of the cell cycle (G1, S, G2, and mitosis) but is absent from resting cells (G0). (fishersci.com)
  • Ki-67 is commonly used as a proliferation marker because it is not detected in G0 cells, but increases steadily from G1 through mitosis. (fishersci.com)
  • The growth fraction can be evaluated using immunohistochemical techniques employing the Ki-67 monoclonal antibody (MoAb) that marks a nuclear protein present in cycling cells. (elsevier.com)
  • The proportion of Ki-67- positive cells was determined on snap-frozen thin tissue sections. (elsevier.com)
  • Proliferating endometrial cells express the Ki-67 antigen. (clinicaltrials.gov)
  • At least 5 high power fields were scored in this manner and an aggregate percent Ki-67 positive cells was reported. (clinicaltrials.gov)
  • This characteristic makes Ki-67 an excellent marker for proliferating cells and is commonly used as one of the prognostic factors in cancer studies. (biolegend.com)
  • Due to strict association of Ki-67 with proliferating cells, Ki-67 is often used as an indicator of the "growth fraction" of a given cell population. (miltenyibiotec.com)
  • It measures the number of cells with Ki-67 antigen in the nucleus . (cancer.ca)
  • Ki-67 is particularly interesting not only because of its use as a clinical tumor marker, but because we find that it links cell cycle progression and heterochromatin maintenance in p21 checkpoint-proficient human cells (Sun et al. (umassmed.edu)
  • Ki-67 mutant mice developed normally and cells lacking Ki-67 proliferated efficiently. (wikipedia.org)
  • The fraction of Ki-67-positive tumor cells (the Ki-67 labeling index) is often correlated with the clinical course of cancer. (wikipedia.org)
  • Protein Ki-67 in human MCF-7 cells. (wikipedia.org)
  • Ki-67 protein (red), tubulin (green) and DNA (blue) in HeLa cells. (wikipedia.org)
  • In situ staining demonstrated virus capsid antigen in koilocytotic cells and surrounding cells in the hyperplastic epithelial layer. (iospress.com)
  • Immunohistochemical analysis of formalin-fixed paraffin-embedded human tonsil using Ki-67 antibody. (genetex.com)
  • The subcellular localization of chmadrin was found to be similar to that of pKi-67 throughout the cell cycle, that is, predominantly nucleolar during interphase and perichromosomal in the mitotic phase. (biologists.org)
  • During interphase, the Ki-67 antigen can be exclusively detected within the cell nucleus, whereas in mitosis most of the protein is relocated to the surface of the chromosomes. (wikipedia.org)
  • Ki-67 proliferative index was 2.80% in the original tumor , 4.40% in the relapse , and 4.45% in the metastasis . (bvsalud.org)
  • A high Ki-67 proliferative index in a pituitary adenoma might indicate a more aggressive behavior . (bvsalud.org)
  • NETs with a low Ki-67 index have a more favourable prognosis and longer survival than those with a high Ki-67 index. (cancer.ca)
  • Each lot of this antibody is quality control tested by our Ki-67 staining protocol below. (biolegend.com)
  • No. 561284) according to the BD Biosciences support protocol, Flow Cytometry Staining Protocol for Detection of Ki-67. (bdbiosciences.com)
  • BACKGROUND: The immunohistochemical (IHC) 4+C score is a cost-effective prognostic tool that uses clinicopathologic factors and four standard IHC assays: oestrogen receptor (ER), PR, HER2 and Ki67. (icr.ac.uk)
  • Prognostic value of automated KI67 scoring in breast cancer: a centralised evaluation of 8088 patients from 10 study groups. (icr.ac.uk)
  • Chapter 3 Prognostic value of cell proliferation (Ki-67 antigen) and nuclear DNA content in clinically resectable, distal bile duct carcinoma. (uva.nl)
  • Tumour cell proliferation (Ki-67) in non-small cell lung cancer: a critical reappraisal of its prognostic role. (springer.com)
  • The same pattern of antigen correlation with progression was found with 40 human gastric carcinomas. (nih.gov)
  • A positive correlation (P less than 0.001) was found for the immunoreactivity of all three antigens in the invasive carcinomas compared with the normal epithelium. (nih.gov)
  • There is a strong linear correlation between Ki-67 proliferative activity and Barrett's carcinogenesis. (ufrgs.br)
  • The correlation between low Ki67 index and histologically low-grade tumors is strong. (biomol.com)
  • Ki67 antigen is the prototypic cell cycle related nuclear protein, expressed by proliferating. (biomol.com)
  • Ki-67 is a nuclear protein that is expressed during various stages in the cell cycle, particularly during late G1, S, G2, and M phases. (fishersci.com)
  • Antigen Ki-67 is a nuclear protein expressed as two isoforms with molecular weights of 395 and 345 kD. (biolegend.com)
  • Neural antigens in oligodendrogliomas and dysembryoplastic neuroepithelial tumors. (nih.gov)
  • In patients with cancer, Ki-67 was not associated with clinical or surgical staging. (ufrgs.br)
  • Development of a Ki-67-based clinical trial assay for neoadjuvant endocrine therapy response monitoring in breast cancer. (icr.ac.uk)
  • The purpose of this study was to evaluate the clinical utility of the Ki-67 MoAb for predicting survival. (elsevier.com)
  • MIB-1 is used in clinical applications to determine the Ki-67 labelling index. (wikipedia.org)
  • Within Ki67 repeats there is a highly conserved new motif of 66 bp named the "Ki67 motif" (Schlüter et al. (atlasgeneticsoncology.org)
  • The deduced amino acid sequence revealed structural homologies in several limited regions to the Ki-67 antigen (pKi-67). (biologists.org)
  • BACKGROUND: A variety of markers, including Ki67, estrogen receptors (ER), and progesterone receptors (PgR), are frequently measured in fine needle aspirates from human breast carcinomas. (icr.ac.uk)
  • Description: The monoclonal antibody 20Raj1 recognizes the human Ki-67 protein. (fishersci.com)
  • Synthetic peptide made to an internal portion of the human Ki67 protein (within residues 1200-1300). (novusbio.com)
  • Synthetic peptide conjugated to KLH derived from within residues 1200 - 1300 of Human Ki67. (abcam.com)
  • Synthetic peptide within Human Ki67 aa 2300-2400 (C terminal). (abcam.com)
  • Human T leukemia cell line, Jurkat, fixed and permeabilized with 70% ethanol, then intracellularly stained with Ki-67 PE and counterstained with 7-AAD (Cat No. 420404) for DNA staining. (biolegend.com)
  • Resting (dashed line) or PHA-activated human peripheral blood lymphocytes (day-3, solid line) fixed and permeabilized with 70% ethanol, then intracellularly stained with Ki-67 PE. (biolegend.com)
  • The antiserum was produced against synthesized peptide derived between 3207-3256 amino acids from the C-Terminal region of human Ki67. (avivasysbio.com)
  • Synthetic peptide from the human Ki-67 allantigen. (genetex.com)
  • This monoclonal antibody 20Raj1 recognizes canine Ki-67. (fishersci.com)
  • Clone REA183 recognizes the Ki-67 antigen, a nuclear and nucleolar protein, which is strictly associated with cell proliferation. (miltenyibiotec.com)
  • Ki-67 (protein) has been shown to interact with CBX3. (wikipedia.org)
  • CBX3 has been shown to interact with PIM1, Ki-67, Lamin B receptor, CBX5 and CBX1. (wikipedia.org)
  • Originally discovered employing mouse monoclonal antibody against a nuclear antigen from Hodgkin's lymphoma-derived cell line, this non-histone protein was named Ki67 after researcher's location (Gerdes and colleagues), Ki for Kiel University in Germany and 67 referring to the clone number on the 96-well plate. (novusbio.com)
  • The 6845 bp 13th exon contains the characteristic sixteen homologous "Ki67 repeats", each of them having length of 366 bp. (atlasgeneticsoncology.org)