Keratinocytes
Epidermal cells which synthesize keratin and undergo characteristic changes as they move upward from the basal layers of the epidermis to the cornified (horny) layer of the skin. Successive stages of differentiation of the keratinocytes forming the epidermal layers are basal cell, spinous or prickle cell, and the granular cell.
Epidermis
The external, nonvascular layer of the skin. It is made up, from within outward, of five layers of EPITHELIUM: (1) basal layer (stratum basale epidermidis); (2) spinous layer (stratum spinosum epidermidis); (3) granular layer (stratum granulosum epidermidis); (4) clear layer (stratum lucidum epidermidis); and (5) horny layer (stratum corneum epidermidis).
Skin
Cells, Cultured
Keratins
A class of fibrous proteins or scleroproteins that represents the principal constituent of EPIDERMIS; HAIR; NAILS; horny tissues, and the organic matrix of tooth ENAMEL. Two major conformational groups have been characterized, alpha-keratin, whose peptide backbone forms a coiled-coil alpha helical structure consisting of TYPE I KERATIN and a TYPE II KERATIN, and beta-keratin, whose backbone forms a zigzag or pleated sheet structure. alpha-Keratins have been classified into at least 20 subtypes. In addition multiple isoforms of subtypes have been found which may be due to GENE DUPLICATION.
Psoriasis
A common genetically determined, chronic, inflammatory skin disease characterized by rounded erythematous, dry, scaling patches. The lesions have a predilection for nails, scalp, genitalia, extensor surfaces, and the lumbosacral region. Accelerated epidermopoiesis is considered to be the fundamental pathologic feature in psoriasis.
Ultraviolet Rays
That portion of the electromagnetic spectrum immediately below the visible range and extending into the x-ray frequencies. The longer wavelengths (near-UV or biotic or vital rays) are necessary for the endogenous synthesis of vitamin D and are also called antirachitic rays; the shorter, ionizing wavelengths (far-UV or abiotic or extravital rays) are viricidal, bactericidal, mutagenic, and carcinogenic and are used as disinfectants.
Keratin-10
Keratin-14
Cell Differentiation
Skin, Artificial
Synthetic material used for the treatment of burns and other conditions involving large-scale loss of skin. It often consists of an outer (epidermal) layer of silicone and an inner (dermal) layer of collagen and chondroitin 6-sulfate. The dermal layer elicits new growth and vascular invasion and the outer layer is later removed and replaced by a graft.
Hair Follicle
A tube-like invagination of the EPIDERMIS from which the hair shaft develops and into which SEBACEOUS GLANDS open. The hair follicle is lined by a cellular inner and outer root sheath of epidermal origin and is invested with a fibrous sheath derived from the dermis. (Stedman, 26th ed) Follicles of very long hairs extend into the subcutaneous layer of tissue under the SKIN.
Melanocytes
Mammalian pigment cells that produce MELANINS, pigments found mainly in the EPIDERMIS, but also in the eyes and the hair, by a process called melanogenesis. Coloration can be altered by the number of melanocytes or the amount of pigment produced and stored in the organelles called MELANOSOMES. The large non-mammalian melanin-containing cells are called MELANOPHORES.
RNA, Messenger
RNA sequences that serve as templates for protein synthesis. Bacterial mRNAs are generally primary transcripts in that they do not require post-transcriptional processing. Eukaryotic mRNA is synthesized in the nucleus and must be exported to the cytoplasm for translation. Most eukaryotic mRNAs have a sequence of polyadenylic acid at the 3' end, referred to as the poly(A) tail. The function of this tail is not known for certain, but it may play a role in the export of mature mRNA from the nucleus as well as in helping stabilize some mRNA molecules by retarding their degradation in the cytoplasm.
Skin Physiological Phenomena
Oncogene Proteins, Viral
Cell Line, Transformed
Cell Division
Papillomavirus E7 Proteins
ONCOGENE PROTEINS from papillomavirus that deregulate the CELL CYCLE of infected cells and lead to NEOPLASTIC CELL TRANSFORMATION. Papillomavirus E7 proteins have been shown to interact with various regulators of the cell cycle including RETINOBLASTOMA PROTEIN and certain cyclin-dependent kinase inhibitors.
Papillomaviridae
A family of small, non-enveloped DNA viruses infecting birds and most mammals, especially humans. They are grouped into multiple genera, but the viruses are highly host-species specific and tissue-restricted. They are commonly divided into hundreds of papillomavirus "types", each with specific gene function and gene control regions, despite sequence homology. Human papillomaviruses are found in the genera ALPHAPAPILLOMAVIRUS; BETAPAPILLOMAVIRUS; GAMMAPAPILLOMAVIRUS; and MUPAPILLOMAVIRUS.
Mouth Mucosa
Lining of the ORAL CAVITY, including mucosa on the GUMS; the PALATE; the LIP; the CHEEK; floor of the mouth; and other structures. The mucosa is generally a nonkeratinized stratified squamous EPITHELIUM covering muscle, bone, or glands but can show varying degree of keratinization at specific locations.
Fibroblasts
Dermis
Desmosomes
A type of junction that attaches one cell to its neighbor. One of a number of differentiated regions which occur, for example, where the cytoplasmic membranes of adjacent epithelial cells are closely apposed. It consists of a circular region of each membrane together with associated intracellular microfilaments and an intercellular material which may include, for example, mucopolysaccharides. (From Glick, Glossary of Biochemistry and Molecular Biology, 1990; Singleton & Sainsbury, Dictionary of Microbiology and Molecular Biology, 2d ed)
Gene Expression
Gene Expression Regulation
Transglutaminases
Transglutaminases catalyze cross-linking of proteins at a GLUTAMINE in one chain with LYSINE in another chain. They include keratinocyte transglutaminase (TGM1 or TGK), tissue transglutaminase (TGM2 or TGC), plasma transglutaminase involved with coagulation (FACTOR XIII and FACTOR XIIIa), hair follicle transglutaminase, and prostate transglutaminase. Although structures differ, they share an active site (YGQCW) and strict CALCIUM dependence.
Papilloma
Signal Transduction
The intracellular transfer of information (biological activation/inhibition) through a signal pathway. In each signal transduction system, an activation/inhibition signal from a biologically active molecule (hormone, neurotransmitter) is mediated via the coupling of a receptor/enzyme to a second messenger system or to an ion channel. Signal transduction plays an important role in activating cellular functions, cell differentiation, and cell proliferation. Examples of signal transduction systems are the GAMMA-AMINOBUTYRIC ACID-postsynaptic receptor-calcium ion channel system, the receptor-mediated T-cell activation pathway, and the receptor-mediated activation of phospholipases. Those coupled to membrane depolarization or intracellular release of calcium include the receptor-mediated activation of cytotoxic functions in granulocytes and the synaptic potentiation of protein kinase activation. Some signal transduction pathways may be part of larger signal transduction pathways; for example, protein kinase activation is part of the platelet activation signal pathway.
Pemphigus
Apoptosis
One of the mechanisms by which CELL DEATH occurs (compare with NECROSIS and AUTOPHAGOCYTOSIS). Apoptosis is the mechanism responsible for the physiological deletion of cells and appears to be intrinsically programmed. It is characterized by distinctive morphologic changes in the nucleus and cytoplasm, chromatin cleavage at regularly spaced sites, and the endonucleolytic cleavage of genomic DNA; (DNA FRAGMENTATION); at internucleosomal sites. This mode of cell death serves as a balance to mitosis in regulating the size of animal tissues and in mediating pathologic processes associated with tumor growth.
Cell Movement
Fibroblast Growth Factor 7
Desmoglein 3
Hemidesmosomes
An anchoring junction of the cell to a non-cellular substrate, similar in morphology to halves of DESMOSOMES. They are composed of specialized areas of the plasma membrane where INTERMEDIATE FILAMENTS bind on the cytoplasmic face to the transmembrane linkers, INTEGRINS, via intracellular attachment proteins, while the extracellular domain of the integrins binds to EXTRACELLULAR MATRIX PROTEINS.
Hair
Up-Regulation
Dermatitis, Atopic
A chronic inflammatory genetically determined disease of the skin marked by increased ability to form reagin (IgE), with increased susceptibility to allergic rhinitis and asthma, and hereditary disposition to a lowered threshold for pruritus. It is manifested by lichenification, excoriation, and crusting, mainly on the flexural surfaces of the elbow and knee. In infants it is known as infantile eczema.
Intermediate Filament Proteins
Keratolytic Agents
Keratin-1
Sebaceous Glands
Small, sacculated organs found within the DERMIS. Each gland has a single duct that emerges from a cluster of oval alveoli. Each alveolus consists of a transparent BASEMENT MEMBRANE enclosing epithelial cells. The ducts from most sebaceous glands open into a HAIR FOLLICLE, but some open on the general surface of the SKIN. Sebaceous glands secrete SEBUM.
Immunohistochemistry
Non-Fibrillar Collagens
Skin Transplantation
Integrin beta4
Carcinoma, Squamous Cell
Acantholysis
Mice, Transgenic
Tetradecanoylphorbol Acetate
Promoter Regions, Genetic
Transfection
Calcium
A basic element found in nearly all organized tissues. It is a member of the alkaline earth family of metals with the atomic symbol Ca, atomic number 20, and atomic weight 40. Calcium is the most abundant mineral in the body and combines with phosphorus to form calcium phosphate in the bones and teeth. It is essential for the normal functioning of nerves and muscles and plays a role in blood coagulation (as factor IV) and in many enzymatic processes.
Langerhans Cells
Recirculating, dendritic, antigen-presenting cells containing characteristic racket-shaped granules (Birbeck granules). They are found principally in the stratum spinosum of the EPIDERMIS and are rich in Class II MAJOR HISTOCOMPATIBILITY COMPLEX molecules. Langerhans cells were the first dendritic cell to be described and have been a model of study for other dendritic cells (DCs), especially other migrating DCs such as dermal DCs and INTERSTITIAL DENDRITIC CELLS.
Cell Transformation, Neoplastic
Epidermolysis Bullosa Simplex
Human papillomavirus 16
Epithelium
Caspase 14
Coculture Techniques
Reverse Transcriptase Polymerase Chain Reaction
9,10-Dimethyl-1,2-benzanthracene
Epidermal Growth Factor
A 6-kDa polypeptide growth factor initially discovered in mouse submaxillary glands. Human epidermal growth factor was originally isolated from urine based on its ability to inhibit gastric secretion and called urogastrone. Epidermal growth factor exerts a wide variety of biological effects including the promotion of proliferation and differentiation of mesenchymal and EPITHELIAL CELLS. It is synthesized as a transmembrane protein which can be cleaved to release a soluble active form.
Blotting, Western
Pemphigus, Benign Familial
An autosomal dominantly inherited skin disorder characterized by recurrent eruptions of vesicles and BULLAE mainly on the neck, axillae, and groin. Mutations in the ATP2C1 gene (encoding the secretory pathway Ca2++/Mn2++ ATPase 1 (SPCA1)) cause this disease. It is clinically and histologically similar to DARIER DISEASE - both have abnormal, unstable DESMOSOMES between KERATINOCYTES and defective CALCIUM-TRANSPORTING ATPASES. It is unrelated to PEMPHIGUS VULGARIS though it closely resembles that disease.
Desmoplakins
Cell Survival
DNA-Binding Proteins
Transcription Factors
Keratin-15
Keratin-17
Epidermolysis Bullosa, Junctional
Form of epidermolysis bullosa having onset at birth or during the neonatal period and transmitted through autosomal recessive inheritance. It is characterized by generalized blister formation, extensive denudation, and separation and cleavage of the basal cell plasma membranes from the basement membrane.
Keratin-5
Base Sequence
Transcription, Genetic
Molecular Sequence Data
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
Down-Regulation
Phosphorylation
Integrin alpha6
An integrin alpha subunit that primarily associates with INTEGRIN BETA1 or INTEGRIN BETA4 to form laminin-binding heterodimers. Integrin alpha6 has two alternatively spliced isoforms: integrin alpha6A and integrin alpha6B, which differ in their cytoplasmic domains and are regulated in a tissue-specific and developmental stage-specific manner.
Ichthyosis, Lamellar
A chronic, congenital ichthyosis inherited as an autosomal recessive trait. Infants are usually born encased in a collodion membrane which sheds within a few weeks. Scaling is generalized and marked with grayish-brown quadrilateral scales, adherent at their centers and free at the edges. In some cases, scales are so thick that they resemble armored plate.
Repressor Proteins
Desmogleins
Skin Aging
Interleukin-8
Epithelial Cells
Cells that line the inner and outer surfaces of the body by forming cellular layers (EPITHELIUM) or masses. Epithelial cells lining the SKIN; the MOUTH; the NOSE; and the ANAL CANAL derive from ectoderm; those lining the RESPIRATORY SYSTEM and the DIGESTIVE SYSTEM derive from endoderm; others (CARDIOVASCULAR SYSTEM and LYMPHATIC SYSTEM) derive from mesoderm. Epithelial cells can be classified mainly by cell shape and function into squamous, glandular and transitional epithelial cells.
Tumor Suppressor Protein p53
Dermatitis, Allergic Contact
Mice, Knockout
Strains of mice in which certain GENES of their GENOMES have been disrupted, or "knocked-out". To produce knockouts, using RECOMBINANT DNA technology, the normal DNA sequence of the gene being studied is altered to prevent synthesis of a normal gene product. Cloned cells in which this DNA alteration is successful are then injected into mouse EMBRYOS to produce chimeric mice. The chimeric mice are then bred to yield a strain in which all the cells of the mouse contain the disrupted gene. Knockout mice are used as EXPERIMENTAL ANIMAL MODELS for diseases (DISEASE MODELS, ANIMAL) and to clarify the functions of the genes.
Enzyme Activation
Fluorescent Antibody Technique
Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
Differential regulation of the human nidogen gene promoter region by a novel cell-type-specific silencer element. (1/6758)
Transfection analyses of the human nidogen promoter region in nidogen-producing fibroblasts from adult skin revealed multiple positive and negative cis-acting elements controlling nidogen gene expression. Characterization of the positive regulatory domains by gel mobility-shift assays and co-transfection studies in Drosophila SL2 cells unequivocally demonstrated that Sp1-like transcription factors are essential for a high expression of the human nidogen gene. Analysis of the negative regulatory domains identified a novel silencer element between nt -1333 and -1322, which is bound by a distinct nuclear factor, by using extracts from adult but not from embryonal fibroblasts. In embryonal fibroblasts, which express significantly higher amounts of nidogen mRNA as compared with adult fibroblasts, this inhibitory nidogen promoter region did not affect nidogen and SV40 promoter activities. The silencer element seems to be active only in nidogen-producing cells. Therefore this regulatory element might function in vivo to limit nidogen gene expression in response to external stimuli. However, none of the identified regulatory elements, including the silencer, contribute significantly to cell-specific expression of the human nidogen gene. Instead we provide evidence that gene expression in epidermal keratinocytes that are not producing nidogen is repressed by methylation-specific and chromatin-dependent mechanisms. (+info)The integrin alpha v beta 6 binds and activates latent TGF beta 1: a mechanism for regulating pulmonary inflammation and fibrosis. (2/6758)
Transforming growth factor beta (TGF beta) family members are secreted in inactive complexes with a latency-associated peptide (LAP), a protein derived from the N-terminal region of the TGF beta gene product. Extracellular activation of these complexes is a critical but incompletely understood step in regulation of TGF beta function in vivo. We show that TGF beta 1 LAP is a ligand for the integrin alpha v beta 6 and that alpha v beta 6-expressing cells induce spatially restricted activation of TGF beta 1. This finding explains why mice lacking this integrin develop exaggerated inflammation and, as we show, are protected from pulmonary fibrosis. These data identify a novel mechanism for locally regulating TGF beta 1 function in vivo by regulating expression of the alpha v beta 6 integrin. (+info)Murine matrix metalloproteinase 9 gene. 5'-upstream region contains cis-acting elements for expression in osteoclasts and migrating keratinocytes in transgenic mice. (3/6758)
Knowledge about the regulation of cell lineage-specific expression of extracellular matrix metalloproteinases is limited. In the present work, the murine matrix metalloproteinase 9 (MMP-9) gene was shown to contain 13 exons, and the 2.8-kilobase pair upstream region was found to contain several common promoter elements including a TATA box-like motif, three GC boxes, four AP-1-like binding sites, an AP-2 site, and three PEA3 consensus sequences that may be important for basic activity of the gene. In order to identify cell-specific regulatory elements, constructs containing varying lengths of the upstream region in front of a LacZ reporter gene were made and studied for expression in transgenic mice generated by microinjection into fertilized oocytes. Analyses of the mice revealed that the presence of sequences between -2722 and -7745 allowed for expression in osteoclasts and migrating keratinocytes, i. e. cells that have been shown to normally express the enzyme in vivo. The results represent the first in vivo demonstration of the location of cell-specific control elements in a matrix metalloproteinase gene and show that element(s) regulating most cell-specific activities of 92-kDa type collagenase are located in the -2722 to -7745 base pair region. (+info)The L1 major capsid protein of human papillomavirus type 11 recombinant virus-like particles interacts with heparin and cell-surface glycosaminoglycans on human keratinocytes. (4/6758)
The L1 major capsid protein of human papillomavirus (HPV) type 11, a 55-kDa polypeptide, forms particulate structures resembling native virus with an average particle diameter of 50-60 nm when expressed in the yeast Saccharomyces cerevisiae. We show in this report that these virus-like particles (VLPs) interact with heparin and with cell-surface glycosaminoglycans (GAGs) resembling heparin on keratinocytes and Chinese hamster ovary cells. The binding of VLPs to heparin is shown to exhibit an affinity comparable to that of other identified heparin-binding proteins. Immobilized heparin chromatography and surface plasmon resonance were used to show that this interaction can be specifically inhibited by free heparin and dextran sulfate and that the effectiveness of the inhibitor is related to its molecular weight and charge density. Sequence comparison of nine human L1 types revealed a conserved region of the carboxyl terminus containing clustered basic amino acids that bear resemblance to proposed heparin-binding motifs in unrelated proteins. Specific enzymatic cleavage of this region eliminated binding to both immobilized heparin and human keratinocyte (HaCaT) cells. Removal of heparan sulfate GAGs on keratinocytes by treatment with heparinase or heparitinase resulted in an 80-90% reduction of VLP binding, whereas treatment of cells with laminin, a substrate for alpha6 integrin receptors, provided minimal inhibition. Cells treated with chlorate or substituted beta-D-xylosides, resulting in undersulfation or secretion of GAG chains, also showed a reduced affinity for VLPs. Similarly, binding of VLPs to a Chinese hamster ovary cell mutant deficient in GAG synthesis was shown to be only 10% that observed for wild type cells. This report establishes for the first time that the carboxyl-terminal portion of HPV L1 interacts with heparin, and that this region appears to be crucial for interaction with the cell surface. (+info)C5a receptor and interleukin-6 are expressed in tissue macrophages and stimulated keratinocytes but not in pulmonary and intestinal epithelial cells. (5/6758)
The anaphylatoxin derived from the fifth component of the human complement system (C5a) mediates its effects by binding to a single high-affinity receptor (C5aR/CD88), the expression of which has been traditionally thought to be restricted to granulocytes, monocytes, macrophages (Mphi), and cell lines of myeloid origin. Recent immunohistochemical data suggested that human bronchial and alveolar cells express C5aR as well. To reexamine the tissue distribution of human C5aR expression, transcription of the C5aR gene was investigated in normal and pathologically affected human lung (bronchopneumonia, tuberculosis), large intestine (acute appendicitis, Crohn's disease), and skin (pyogenic granuloma, lichen planus) using in situ hybridization. In contrast to previous evidence, C5aR mRNA could not be detected in pulmonary or intestinal epithelial cells, whereas keratinocytes in inflamed but not in normal skin revealed detectable levels of C5aR transcripts. Additionally, it could be documented that only migrating Mphi express C5aR mRNA, whereas sessile Mphi in normal tissues and epithelioid/multinucleated Mphi found in granulomatous lesions do not. Because C5a has been demonstrated to upregulate the expression of interleukin (IL)-6 in human monocytes, we also studied IL-6 gene transcription in parallel to the C5aR. IL-6 mRNA was detectable in many tissue Mphi. Surprisingly, a tight co-expression of C5aR and IL-6 mRNA was observed in keratinocytes from lesions of pyogenic granuloma and lichen planus. These results point to an as yet unknown role for C5a in the pathogenesis of skin disorders beyond its well-defined function as a chemoattractant and activator of leukocytes. (+info)CCAAT/enhancer-binding proteins. A role in regulation of human involucrin promoter response to phorbol ester. (6/6758)
The phorbol ester 12-O-tetradecanoylphorbol-13-acetate (TPA) is a potent inducer of keratinocyte differentiation and of involucrin gene expression. In the present study we show that a CCAAT/enhancer-binding protein (C/EBP) site in the proximal regulatory region is required for the phorbol ester response. Mutation of the C/EBP site results in the loss of basal and TPA-responsive activity. Gel mobility supershift analysis shows that C/EBPalpha binding to this site is increased by TPA treatment. Moreover, cotransfection of the human involucrin reporter plasmid with C/EBPalpha increases promoter activity to an extent comparable with TPA treatment. Mutation of the C/EBP-binding site eliminates these responses. Transfection experiments using GADD153 to create C/EBP-null conditions confirm that C/EBP factors are absolutely required for promoter activity and TPA responsiveness. C/EBPbeta and C/EBPdelta inhibit both TPA- and C/EBPalpha-dependent promoter activation, indicating functional differences among C/EBP family members. These results suggest that C/EBP transcription factor activity is necessary for basal promoter activity and TPA response of the involucrin gene. (+info)UV-A-induced decrease in nuclear factor-kappaB activity in human keratinocytes. (7/6758)
Previous reports have demonstrated an increase in nuclear factor-kappaB (NF-kappaB) activity in response to UV radiation. These studies have essentially focused on the DNA-damaging fraction of solar UV radiation (UV-B and UV-C). In contrast, the effects of UV-A radiation (320-400 nm) on NF-kappaB are not well known. In this study, we present evidence that UV-A radiation induces a marked decrease in NF-kappaB DNA-binding activity in NCTC 2544 human keratinocytes. In addition, NCTC 2544 keratinocytes pretreated with UV-A fail to respond to NF-kappaB inducers. Moreover, UV-A radiation induces a decrease in NF-kappaB-driven luciferase reporter gene expression in NCTC 2544 keratinocytes. The expression of the gene encoding IkappaBalpha (IkappaB is the NF-kappaB inhibitor), which is closely associated with NF-kappaB activity, is also reduced (3-fold) upon UV-A treatment. Our results indicate that the UV-A-induced decrease in NF-kappaB DNA-binding activity is associated with a decrease in the levels of the p50 and p65 protein subunits. This is the first evidence that an oxidative stress, such as UV-A radiation, may induce a specific decrease in NF-kappaB activity in mammalian cells, probably through degradation of NF-kappaB protein subunits. These findings suggest that UV-A could modulate the NF-kappaB-dependent gene expression. (+info)Psoriatic keratinocytes show reduced IRF-1 and STAT-1alpha activation in response to gamma-IFN. (8/6758)
Psoriasis is a chronic inflammatory dermatosis characterized by hyperproliferative keratinocytes (KC). The skin lesions are infiltrated by T cells, which secrete gamma interferon (gamma-IFN) and are believed to be necessary to maintain the psoriatic phenotype. In normal KC, gamma-IFN is a potent inhibitor of proliferation, but proliferation of KC persists in psoriatic plaques despite the presence of gamma-IFN. Immunostaining of interferon regulatory factor-1 (IRF-1) revealed that IRF-1 was localized to the basal cells of the epidermis in normal and in nonlesional psoriatic skin, but was suprabasal or completely absent in lesional psoriatic skin. This finding led to the hypothesis that abnormal signaling in the gamma-IFN pathway may occur in psoriatic KC. To test this hypothesis, we measured activation of IRF-1 and signal transducer and activator of transcription (STAT)-1alpha transcription factors in KC after stimulation with gamma-IFN. Primary cultures of KC from normal and nonlesional psoriatic skin were stimulated with gamma-IFN and subsequent transcription factor activation was measured by electrophoretic mobility shift assay. Psoriatic KC showed a reduced induction of IRF-1 and STAT-1alpha activation after stimulation with gamma-IFN, compared with normal KC. Reduced activation of IRF-1 and STAT-1alpha in response to gamma-IFN indicates a fundamental defect in the growth and differentiation control of psoriatic KC in the absence of the influence of other cell types. (+info)
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Keratinocyte differentiation markers: involucrin, transglutaminase, and toxicity.
- UC Davis
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The activation of cultured keratinocytes by cholesterol depletion during reconstruction of a human epidermis is reminiscent of...
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Ca<sup>2+</sup> waves in keratinocytes are transmitted to sensory neurons: The involvement of extracellular ATP and P2Y<sub>2<...
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C/EBPδ gene targets in human keratinocytes. - Science Exchange
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FGF receptors 1 and 2 are key regulators of keratinocyte migration in vitro and in wounded skin | Journal of Cell Science
Epidermal keratinocytes play a vital function in restoration from the unchanged skin barrier during wound therapeutic |...
Inhibition of mTORC2 enhances UVB-induced apoptosis in keratinocytes through a mechanism dependent on the FOXO3a...
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Concordant Induction of Cyclin E and p21cip1 in Differentiated Keratinocytes by the Human Papillomavirus E7 Protein Inhibits...
Regulation der Freisetzung von SCF aus proliferierenden versus differenzierenden Keratinozyten/HaCaT
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An immunohistopathological and functional investigation of β3 integrin antagonism as a therapeutic strategy in cancer....
Cooling-mediated protection from chemotherapy drug-induced cytotoxicity in human keratinocytes by inhibition of cellular drug...
Robust HPV-18 production in organotypic cultures of primary human keratinocytes. - NextBio article
Overexpression of bcl-2 protein inhibits terminal differentiation of oral keratinocytes in vitro<...
Melanoma cells influence the differentiation pattern of human epidermal keratinocytes | MEDIC FINDER
Identification of novel tumour-associated genes differentially expressed in the process of squamous cell cancer development |...
Mechanism underlying ATP release in human epidermal keratinocytes<...
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JCI -
Pemphigus autoantibodies generated through somatic mutations target the desmoglein-3 cis-interface
Organized skin structure is regenerated in vivo from collagen-GAG matrices seeded with autologous keratinocytes<...
Ligand-activated PPARδ inhibits UVB-induced senescence of human keratinocytes via PTEN-mediated inhibition of superoxide...
Autologous cultured keratinocytes on porcine gelatin microbeads effectively heal chronic venous leg ulcers - Zurich Open...
Regulation of epidermal growth factor binding in a human keratinocyte cell line by 2,3,7,8-tetrachlorodibenzo-p-dioxin<...
HPV8 Field Cancerization in a Transgenic Mouse Model Is due to Lrig1+ Keratinocyte Stem Cell Expansion. - Medawar
Ecto-nucleoside triphosphate diphosphohydrolase 2 modulates local ATP-induced calcium signaling in human HaCaT keratinocytes. |...
Human lactoferrin stimulates skin keratinocyte function and wound re-epithelialization<...
Organotypic 3D Skin Models: Human Epidermal Equivalent Cultures from Primary Keratinocytes and Immortalized Keratinocyte Cell...
The expression of desmoglein isoforms in cultured human keratinocytes is regulated by calcium, serum, and protein kinase C<...
Involvement of c-JUN in the regulation of terminal differentiation genes in normal and malignant keratinocytes<...
Yokukansan, a Traditional Japanese Medicine, Adjusts Glutamate Signaling in Cultured Keratinocytes
JCI -
Expression, topography, and function of integrin receptors are severely altered in keratinocytes from involved and...
Untargeted metabolomics unveil alterations of biomembranes permeability in human HaCaT keratinocytes upon 60 GHz millimeter...
Melanin Uptake Reduces Cell Proliferation of Human Epidermal Keratinocytes
Immortalization of human foreskin keratinocytes by various human papillomavirus DNAs corresponds to their association with...
Hyaluronan minimizes effects of UV irradiation on human keratinocytes
EGF upregulates, whereas TGF-beta downregulates, the hyaluronan synthases Has2 and Has3 in organotypic keratinocyte cultures:...
CD8<sup>+</sup> T cells mediate RAS-induced psoriasis-like skin inflammation through...
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Identification of an epidermal keratinocyte AMPA glutamate receptor involved in dermatopathies associated with sensory...
Carbonic anhydrases in human keratinocytes and their regulation by all-trans retinoic acid and 1α,25-dihydroxyvitamin D3 -...
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RePub, Erasmus University Repository:
Molecular pathophysiology of psoriasis and molecular targets of antipsoriatic therapy
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Staphylococcus aureus Susceptibility to Innate Antimicrobial Peptides, β-Defensins and CAP18, Expressed by Human Keratinocytes ...
Role of nerve growth factor in rantes expression by keratinocytes<...
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A distal region of the human TGM1 promoter is required for expression in transgenic mice and cultured keratinocytes.
Reconstitution of human epidermis in vitro is accompanied by transient activation of basal keratinocyte spreading<...
Pertussis toxin-sensitive secretory phospholipase A<sub>2</sub> expression and motility in activated primary human...
Modulation of the IL-1 cytokine network in keratinocytes by intracellular IL-1 alpha and IL-1 receptor antagonist. - Nuffield...
Role of Integrin-linked kinase in epidermal integrity and barrier function by Samar Sayedyahossein
CorpusUL: Characterization of wound reepithelialization using a new human tissue-engineered corneal wound healing model
The p63 Gene Is Regulated by Grainyhead-like 2 (GRHL2) through Reciprocal Feedback and Determines the Epithelial Phenotype in...
Interferon-gamma enhances cytotoxic T lymphocyte recognition of endogenous peptide in keratinocytes without lowering the...
DSpace at KOASAS: Exposure of keratinocytes to non-thermal dielectric barrier discharge plasma increases the level of 8...
Browsing School, Graduate by Subject keratinocyte differentiation
Olaf Joseph Franciscus Schreurs
- Institutt for oral biologi
MMP-13 plays a role in keratinocyte migration, angiogenesis, and contraction in mouse skin wound healing<...
Immunopathogenesis of psoriasis - Sabat - 2007 - Experimental Dermatology - Wiley Online Library
Plus it
Intracellular IL-1 Receptor Antagonist Isoform 1 Released from Keratinocytes upon Cell Death Acts as an Inhibitor for the...
THE IDENTIFICATION OF CELLULAR GENES ALTERED BY HTERT AND HPV-16 IN THE IMMORTALIZATION OF HUMAN KERATINOCYTES
Junichi Hachisuka, MD, PhD - Pittsburgh Center for Pain Research
Aging | The life in a gradient: calcium, the lncRNA SPRR2C and mir542/mir196a meet in the epidermis to regulate the aging...
Formyl peptide receptor 1
... skin keratinocytes; and virtually all types of multicellular tissues. Formyl peptide receptor GRCh38: Ensembl release 89: ...
Papillomaviridae
The differentiation of keratinocytes can be mimicked in vitro by exposing cultured keratinocytes to an air/liquid interface. ... Papillomaviruses replicate exclusively in keratinocytes. Keratinocytes form the outermost layers of the skin, as well as some ... Less-differentiated keratinocyte stem cells, replenished on the surface layer, are thought to be the initial target of ... Keratinocyte stem cells in the epithelial basement layer can maintain papillomavirus genomes for decades. The expression of the ...
Wound healing
As keratinocytes continue migrating, new epithelial cells must be formed at the wound edges to replace them and to provide more ... Migration of keratinocytes over the wound site is stimulated by lack of contact inhibition and by chemicals such as nitric ... Keratinocytes continue migrating across the wound bed until cells from either side meet in the middle, at which point contact ... Proliferation behind migrating keratinocytes normally begins a few days after wounding and occurs at a rate that is 17 times ...
Topical steroid withdrawal
Recent research has shown that keratinocytes in human skin also produce cortisol. Prolonged TS application changes the ... Cirillo, N; Prime, S (2011). "Keratinocytes synthesize and activate cortisol". Journal of Cellular Biochemistry. 112 (6): 1499- ...
PTPRK
TGFβ1 is a growth inhibitor in human keratinocytes. Stimulation of the cultured human keratinocyte cell line, HaCaT, with TGFβ1 ... Yang Y, Gil M, Byun SM, Choi I, Pyun KH, Ha H (1996). "Transforming growth factor-beta1 inhibits human keratinocyte ... UV-irradiation of primary human keratinocytes yields the same results, namely a reduction of PTPkappa tyrosine phosphatase ... protein-tyrosine phosphatase kappa by ultraviolet irradiation activates epidermal growth factor receptor in human keratinocytes ...
FGF7
Keratinocyte growth factor is a protein that in humans is encoded by the FGF7 gene. The protein encoded by this gene is a ... Aaronson SA, Bottaro DP, Miki T, Ron D, Finch PW, Fleming TP, Ahn J, Taylor WG, Rubin JS (1992). "Keratinocyte growth factor. A ... Post M, Souza P, Liu J, Tseu I, Wang J, Kuliszewski M, Tanswell AK (Oct 1996). "Keratinocyte growth factor and its receptor are ... Guo L, Degenstein L, Fuchs E (Jan 1996). "Keratinocyte growth factor is required for hair development but not for wound healing ...
Collagen, type XVII, alpha 1
1998). "Two forms of collagen XVII in keratinocytes. A full-length transmembrane protein and a soluble ectodomain". J. Biol. ... Collagen XVII is constitutively shed from the keratinocyte surface within NC16A domain by TACE (TNF-Alpha Converting Enzyme), ... "Two forms of collagen XVII in keratinocytes. A full-length transmembrane protein and a soluble ectodomain". J. Biol. Chem. 273 ... multiprotein complexes at the dermal-epidermal basement membrane zone that mediate adhesion of keratinocytes to the underlying ...
Cannabinoid receptor
They also have a function in keratinocytes. They are also expressed on peripheral nerve terminals. These receptors play a role ...
Toxic epidermal necrolysis
Keratinocytes are the cells found lower in the epidermis and specialize in holding the surrounding skin cells together. It is ... It appears that a certain type of immune cell (cytotoxic CD8+ T cell) is primarily responsible for keratinocyte death and ... CD8+ T cells then mediate keratinocyte cell death through release of a number of molecules, including perforin, granzyme B, and ... Histologically, early TEN shows scattered necrotic keratinocytes. In more advanced TEN, full thickness epidermal necrosis is ...
Stratum spinosum
The tonofibrils go on to form the desmosomes, which allow for strong connections to form between adjacent keratinocytes. The ... This layer is composed of polyhedral keratinocytes. These are joined together with desmosomes. Their spiny (Latin, spinosum) ... although the actual keratinocytes begin in the stratum basale. They have large pale-staining nuclei as they are active in ...
Skin whitening
Keratinocytes carry the melanosomes with them as they move towards the surface. Keratinocytes contribute to skin pigmentation ... Melanosomes along with the melanin they contain are transferred from melanocytes to keratinocytes when keratinocytes are low in ... 2010). "Keratinocytes in culture accumulate phagocytosed melanosomes in the perinuclear area". Pigment Cell Melanoma Res. 23 (1 ... The transfer of melanosomes to keratinocytes is a necessary condition for the visible pigmentation of the skin. Blocking this ...
Growth factor
Keratinocyte growth factor (KGF). *Migration-stimulating factor (MSF). *Macrophage-stimulating protein (MSP), also known as ...
Methylparaben
October 2006). "Methylparaben potentiates UV-induced damage of skin keratinocytes". Toxicology. 227 (1-2): 62-72. doi:10.1016/j ...
Collagen, type XXIII, alpha 1
Both proteins co-localize on basal keratinocytes surface. Collagen XXIII plays a role as a biomarker for detection and ...
TRPV3
The TRPV3 channel has wide tissue expression that is especially high in the skin (keratinocytes) but also in the brain. It ... June 2002). "A heat-sensitive TRP channel expressed in keratinocytes". Science. 296 (5575): 2046-9. doi:10.1126/science.1073140 ... Receptor Potential Vanilloid TRPV3 Channel by Natural Forsythoside B Attenuates Pruritus and Cytotoxicity of Keratinocytes". ...
S100A11
... was localized in the cytoplasm of resting human keratinocytes in vitro. It has been shown to interact with the RAGE ... November 2003). "S100C/A11 is a key mediator of Ca(2+)-induced growth inhibition of human epidermal keratinocytes". The Journal ... January 2008). "S100A11, an dual mediator for growth regulation of human keratinocytes". Molecular Biology of the Cell. 19 (1 ... Sakaguchi M, Huh NH (October 2011). "S100A11, a dual growth regulator of epidermal keratinocytes". Amino Acids. 41 (4): 797-807 ...
SLURP1
Arredondo J, Chernyavsky AI, Webber RJ, Grando SA (December 2005). "Biological effects of SLURP-1 on human keratinocytes". The ...
Apolipoprotein D
"Senescence-associated genes in normal human oral keratinocytes". Experimental Cell Research. 287 (2): 272-81. doi:10.1016/S0014 ...
Tissue engineering
They demonstrated growing a coherent layer of keratinocytes. A persistent problem within tissue engineering is mass transport ...
Fiona Watt
Adams, JC; Watt, FM (1989). "Fibronectin inhibits the terminal differentiation of human keratinocytes". Nature. 340 (6231): 307 ... Zhu, AJ; Watt, FM (1999). "beta-catenin signalling modulates proliferative potential of human epidermal keratinocytes ... where she served as Head of the Keratinocyte Laboratory. From 2007 to 2012 she worked in Cambridge, where she helped to ...
Paracytophagy
Scott, G.; Leopardi, S.; Printup, S.; Madden, B. (2002). "Filopodia are conduits for melanosome transfer to keratinocytes". ... Melanosomes are also transferred by filopodia from melanocytes to keratinocytes. This transfer involves a classic filopodial ...
Corneocyte
... s are keratinocytes in their last stage of differentiation. Keratinocytes in the stratum basale of the epidermis will ... Skin Epidermis (skin) Keratinocyte Elias PM (April 2007). "The skin barrier as an innate immune element". Seminars in ... During that migration keratinocytes will undergo multiple stages of differentiation to finally become corneocytes once they ... Corneocytes are keratinocytes without nuclei and cytoplasmic organelles. They contain a highly insoluble cornified envelope ...
CD47
Only keratinocytes expressed significant amounts of form 1. Little is known about the functional significance of this ...
Oral pigmentation
Melanin is then transferred to keratinocytes in melanosomes. Nevus cells in the skin and oral mucosa also produce melanin. Oral ...
Interleukin-1 family
IL-36γ is most produced by keratinocytes. It activates NF-κB via interleukin 1 receptor-like 2 (IL-1Rrp2) and is specifically ... IL-36ra is highly expressed by keratinocytes, in psoriatic skin, placenta, uterus, brain, kidneys, monocytes, B-lymphocytes and ... It is produced by monocytes, macrophages, osteoblasts, keratinocytes. It is synthesized as an inactive precursor that is ... The intracellular form was found in fibroblasts, monocytes, neutrophils, keratinocytes and bronchial epithelial cells. IL-1ra ...
Platinum nanoparticle
In another study, researcher tested the effects of differently sized platinum nanoparticles on primary human keratinocytes. The ... "Effects triggered by platinum nanoparticles on primary keratinocytes". International Journal of Nanomedicine. 8: 3963-75. doi: ...
Bioelectricity
Nishimura, K. Y; Isseroff, R. R; Nuccitelli, R (1996). "Human keratinocytes migrate to the negative pole in direct current ... Gruler, Hans; Nuccitelli, Richard (2000). "The Galvanotaxis Response Mechanism of Keratinocytes Can Be Modeled as a ... "Kindlin-1 Regulates Keratinocyte Electrotaxis". Journal of Investigative Dermatology. 136 (11): 2229-2239. doi:10.1016/j.jid. ... "The epithelial sodium channel mediates the directionality of galvanotaxis in human keratinocytes". Journal of Cell Science. 126 ...
Cord lining
Pellegrini, G. (2001). p63 identifies keratinocyte stem cells. PNAS, 98(6), 3156-3161. Reza, H. M. (2011). Characterization of ... Ruetze, M. (2008). Common features of umbilical cord epithelial cells and epidermal keratinocytes. Journal of Dermatological ... due to their similarity in terms of phenotypic expression of keratins compared to normal human epidermal keratinocytes, cord ...
Keratin 16
Wu C, Li C, Wei L, Zheng Z (2008). "Innate immune modulation of keratinocytes by antikeratin 16 antibodies". Exp. Dermatol. 17 ... 2008). "Distribution of dipeptidyl-peptidase IV on keratinocytes in the margin zone of a psoriatic lesion: a comparison with ...
C-c motif chemokine ligand 27
... production in keratinocytes is controlled by nuclear factor kappaB". Cytokine. 29 (2): 49-55. doi:10.1016/j.cyto.2004.09.008. ... "TARC augments TNF-alpha-induced CTACK production in keratinocytes". Experimental Dermatology. 13 (9): 551-7. doi:10.1111/j.0906 ...
Keratinocyte growth factor - Wikipedia
The keratinocyte growth factor (KGF), also known as FGF7, is a growth factor present in the epithelialization-phase of wound ... In this phase, keratinocytes are covering the wound, forming the epithelium. KGF is a small signaling molecule that binds to ... "Silencing of Keratinocyte Growth Factor Receptor Restores 5-Fluorouracil and Tamoxifen Efficacy on Responsive Cancer Cells" ... Retrieved from "https://en.wikipedia.org/w/index.php?title=Keratinocyte_growth_factor&oldid=941029836" ...
Keratinocyte | cell | Britannica.com
... with a group of neighbouring keratinocytes (keratin-synthesizing epidermal cells) into which its dendrites transfer pigment. ... The keratinocytes slowly move outward through the epidermis as they mature, and they eventually die and are… ... contains layers of cells called keratinocytes. Only the basal layer, next to the dermis, contains cells that divide. A number ... with a group of neighbouring keratinocytes (keratin-synthesizing epidermal cells) into which its dendrites transfer pigment. ...
Keratinocyte - Wikipedia
Since keratinocyte differentiation inhibits keratinocyte proliferation, factors that promote keratinocyte proliferation should ... Functional keratinocytes are needed for tympanic perforation healing. A sunburn cell is a keratinocyte with a pyknotic nucleus ... Within the epidermis keratinocytes are associated with other cell types such as melanocytes and Langerhans cells. Keratinocytes ... to the developing keratinocytes. Houben E, De Paepe K, Rogiers V (2007). "A keratinocytes course of life". Skin Pharmacology ...
Antigen presentation by keratinocytes directs autoimmune skin disease | PNAS
Keratinocytes as APCs.. The keratinocytes in polyclonal K14 mice are I-A-negative and should be incapable of activating ... Antigen presentation by keratinocytes directs autoimmune skin disease. Lian Fan, Brian W. Busser, Traci Q. Lifsted, David Lo, ... Antigen presentation by keratinocytes directs autoimmune skin disease. Lian Fan, Brian W. Busser, Traci Q. Lifsted, David Lo, ... and these keratinocytes stimulate autoreactive CD4+ cells to produce Th1 cytokines, which drive further keratinocyte ...
p63 identifies keratinocyte stem cells | PNAS
Our findings have major implications for the study of keratinocytes in two different fields. First, cultured keratinocytes ... in K45-AS keratinocytes (H, blue column). (B) Cultured epidermal sheets prepared from antisense-σ-transduced keratinocytes were ... p63 identifies keratinocyte stem cells. Graziella Pellegrini, Elena Dellambra, Osvaldo Golisano, Enrica Martinelli, Ivana ... p63 identifies keratinocyte stem cells. Graziella Pellegrini, Elena Dellambra, Osvaldo Golisano, Enrica Martinelli, Ivana ...
Keratinocyte transglutaminase - Wikipedia
Keratinocyte transglutaminase enzymes serve to specifically catalyze the development of the cornified cell envelope, a defining ... The specific cross linkages formed by keratinocyte transglutaminase are between n^ε-(γ-glutamyl)lysine residues which develop ... A study on the mutation of keratinocyte transglutaminase (TGK) came to conclude that those affected with ichthyosis lamellaris ... Keratinocyte ENSG00000285348 GRCh38: Ensembl release 89: ENSG00000092295, ENSG00000285348 - Ensembl, May 2017 GRCm38: Ensembl ...
Human Keratinocyte Cell Lines | SpringerLink
... immortalization of human epidermal keratinocytes has been generally considered to have a massive impact on their ... HaCaT Cell Human Keratinocytes Normal Keratinocytes Human Epidermal Cell Human Keratinocyte Cell Line These keywords were added ... 1991) Human Keratinocyte Cell Lines. In: Wilson G., Davis S.S., Illum L., Zweibaum A. (eds) Pharmaceutical Applications of Cell ... Fuchs E, Green H (1981) Regulation of terminal differentiation of cultured human keratinocytes by vitamin A. Cell 25: 617-625 ...
Melanoma cells talk to keratinocytes | Science
keratinocyte cell culture
... Courtland Yockey courtland.yockey at mindspring.com Thu Jan 13 15:18:42 EST 2000 *Previous message: ... When last I interacted with them 2 years ago, their labs were doing primary keratinocyte cultures from human newborn foreskins ... I am interested in generating primary keratinocyte cell cultures and would appreciate any advice, references, protocols, and ...
Keratinocytes have the metastatic touch | Science Signaling
Sequencing of the small RNAs in melanoma cells cultured on DLL1-coated plates or cocultured with keratinocytes revealed that ... Compared with mice injected with melanoma cells and either basal keratinocytes or fibroblasts, mice injected with melanoma ... found that contact with differentiated keratinocytes in the upper epidermis triggered vertical invasion by melanoma cells. ... Noninvasive melanoma cells became invasive in Matrigel when cocultured with differentiated keratinocytes but not with basal ...
Escherichia coli ghosts promote innate immune responses in human keratinocytes. - PubMed - NCBI
Primary Epidermal Keratinocytes; Normal, Human, Adult (HEKa) ATCC ®
... when grown in Dermal Cell Basal Media supplemented with Keratinocyte Growth Kit components, provide an ideal cell system to ... propagate keratinocytes in serum-free (not animal free) conditions. The cells are cryopreserved in the first passage to ensure ... Primary Epidermal Keratinocytes; Normal, Human, Adult (HEKa) (ATCC® PCS-200-011™) Organism: Homo sapiens, human / Tissue: skin ... keratinocyte Morphology Cobblestone appearance; cells are rounded, not flat; cells display a high mitotic index; at near 80% ...
Skin cell (keratinocyte)
HPV16 E6 and E7 proteins cooperate to immortalize human foreskin keratinocytes. - PubMed - NCBI
HPV16 E6 and E7 proteins cooperate to immortalize human foreskin keratinocytes.. Hawley-Nelson P1, Vousden KH, Hubbert NL, Lowy ... Therefore, we conclude that HPV16 E6 and E7 cooperative to immortalize human keratinocytes in vitro. Changes in cellular gene ... Co-transfection of a plasmid with an intact E6 ORF and a second plasmid with an intact E7 ORF generated keratinocyte lines with ... induced an indefinite life-span in the keratinocytes with an efficiency similar to that of the entire early region of the viral ...
Yokukansan, a Traditional Japanese Medicine, Adjusts Glutamate Signaling in Cultured Keratinocytes
The aim of this study was to assess the effect of YKS on skin and cultured human keratinocytes. Glutamate concentrations in ... Our findings indicate that YKS affects peripheral glutamate signaling in keratinocytes. Glutamine is essential as a transmitter ... Then, glutamate release from cultured keratinocytes was measured, and extracellular glutamate concentrations in YKS-stimulated ... Glutamate concentrations in cell supernatants of cultured keratinocytes increased proportionally to the cell density. However, ...
Dihydroceramide Desaturase Inhibition by a Cyclopropanated Dihydroceramide Analog in Cultured Keratinocytes
Browsing by Subject "Keratinocytes"
Expression of CD90 on keratinocyte stem/progenitor cells: Ingenta Connect
To investigate the biological function of CD90+ and CD90− keratinocytes. Methods CD90+ and CD90− keratinocytes were purified ... while most of the keratinocytes maintained expression of α6 integrin. Purified CD90+ keratinocytes demonstrated a sixfold ... Here, we report the detection of CD90+ cells in cultured normal human epidermal keratinocytes and adult skin. Objectives ... The identification and purification of keratinocyte stem cells (KSCs) that are capable of self-renewal and maintenance of ...
shRNA to primary keratinocytes - Molecular Cloning
primary keratinocytes - HeKn - Cell Biology - BioForum
primary keratinocytes - HeKn - posted in Cell Biology: Hello! Somebody knows how cultre these cells? We are using original, ... We add 10 ng/mL KGF (from Epoch Biolabs) in keratinocyte growth medium that help a lot. But be careful, KGF from other vendors ... primary keratinocytes - HeKn. Started by bionick, Dec 10 2004 02:53 AM ...
Kv7/M-type potassium channels in rat skin keratinocytes. | Sigma-Aldrich
Kv7/M-type potassium channels in rat skin keratinocytes.. [Joanne M Reilly, Vsevolod Telezhkin, Gayle M Passmore, Stephen J ... Skin keratinocytes fulfil important signalling and protective functions. Immunocytochemical experiments revealed the unexpected ... Application of the M-channel enhancer retigabine (3-10 μM) to isolated cultured rat keratinocytes: (a) increased outward ... We conclude that rat skin keratinocytes possess M-channels that, when activated, can modify their physiological properties, ...
increased keratinocyte apoptosis Mammalian Phenotype Term (MP:0009580)
The Mammalian Phenotype (MP) Ontology is a community effort to provide standard terms for annotating phenotypic data. You can use this browser to view terms, definitions, and term relationships in a hierarchical display. Links to summary annotated phenotype data at MGI are provided in Term Detail reports.
Keratinocytes drive melanoma invasion in a reconstructed skin model.
Keratinocytes / pathology, physiology*. Melanoma / pathology*. Models, Biological. Neoplasm Invasiveness. Neoplasm Metastasis. ... The dynamic interplay between keratinocytes and melanoma cells is further shown by an altered growth pattern of melanoma cells ... but that the interplay between surrounding keratinocytes and the melanoma cells plays an important role in melanoma invasion.. ... is secreted by the keratinocytes and subsequently activated by an unknown soluble factor secreted by the melanoma cells. ...
Aldefluor protocol to sort keratinocytes stem cells from skin
After keratinocytes isolation and culture, when the cell confluence reached 80%, the keratinocytes were transferred to new ... which are well known for other cell lines but not for keratinocytes. Since no specific protocol for epidermal keratinocytes was ... the definition of a protocol for isolated keratinocyte stem cells was pivotal. The importance of flow cytometry in keratinocyte ... to design a protocol to sort keratinocyte stem cells from cultured keratinocytes from burned patients. ...
Dynamic and Physical Clustering of Gene Expression during Epidermal Barrier Formation in Differentiating Keratinocytes
The keratinocyte is its principal cell. Keratinocyte proteins form a physical epithelial barrier, protect against microbial ... We measured global gene expression in triplicate at five times over the ten days that the keratinocytes took to fully ... The results provide multiple novel insights into keratinocyte biology, in particular providing a comprehensive list of known ... Gene Ontology analyses showed that undifferentiated keratinocytes were characterised by genes for motility and the adaptive ...
Experts and Doctors on keratinocytes in Germany
Species about Experts and Doctors on keratinocytes in Germany ... Crosstalk between keratinocytes and adaptive immune cells in an ... HaCaT keratinocytes overexpressing the S100 proteins S100A8 and S100A9 show increased NADPH oxidase and NF-kappaB activities. J ... Keratinocytes determine Th1 immunity during early experimental leishmaniasis. PLoS Pathog. 2010;6:e1000871 pubmed publisher ... Qu H, Wen T, Pesch M, Aumailley M. Partial loss of epithelial phenotype in kindlin-1-deficient keratinocytes. Am J Pathol. 2012 ...
17beta-estradiol Inhibits the Production of RANTES in Human Keratinocytes
The production of RANTES is enhanced in keratinocytes of psoriatic skin lesions, which may contribute to the inflammatory ... 17beta-estradiol Inhibits the Production of RANTES in Human Keratinocytes J Invest Dermatol. 2003 Mar;120(3):420-7. doi: ... The production of RANTES is enhanced in keratinocytes of psoriatic skin lesions, which may contribute to the inflammatory ... We examined the in vitro effects of 17beta-estradiol on RANTES production by human keratinocytes. 17beta-estradiol inhibited ...
Oxidative Damage in Keratinocytes Exposed to Cigarette Smoke and Aldehydes
... Toxicol In Vitro. 2014 Jun;28(4):485-91. doi: ... Intracellular protein carbonyl modification under CS, acrolein and acetaldehyde exposure in the HaCaT keratinocyte cell line, ... Keywords: Cigarette smoke; Glutathione; Keratinocytes; Oxidative stress; Protein carbonylation; α,β-Unsaturated aldehydes. ... representing oral keratinocytes was examined by Western blot. Possible intracellular enzymatic dysfunction under the above ...
Untargeted metabolomics unveil alterations of biomembranes permeability in human HaCaT keratinocytes upon 60 GHz millimeter...
For this purpose, HaCaT human keratinocytes were exposed at 60.4 GHz with an incident power density of 20 mW/cm², this value ... HaCaT keratinocytes cell viability was formerly reported to be of 100% for pH values spanning across the 7.0-8.2 range41. ... A human keratinocyte cell line (HaCaT) was cultured as described elsewhere22. In an attempt to circumvent any senescence or ... For this purpose, HaCaT human keratinocytes were exposed at 60.4 GHz with an incident power density of 20 mW/cm², this value ...
Testing Tactics in Skin: Keratinocytes as Sensory Nociceptors
It is these keratinocytes that form a small barrier between the nociceptive nerve fibers and the outside. Interestingly, these ... Rather, it can be harmful to the keratinocyte and/or to the sensory neurons associated with it. It is the counter-action of ... Both epidermal keratinocytes and epidermal nociceptive sensory nerves use a common family of proteins to detect noxious stimuli ... Hesselink, J.M.K., Kopsky, D.J. and Bhaskar, A.K. (2017). Skin matters! The role of keratinocytes in nociception: a rational ...
HaCaTDifferentiationEpidermisOxidativeCellsMelanocytesMouse keratinocytesProtects keratinocytesVitroCellHumansApoptosis in epidermalMiceCytokinesEpithelialIsolated culturedInhibitionFibroblastsOral keratinocytesProteinsPsoriasisLesionsSkin and culturedPrimary keratinocyteCorneal keratinocytesGrowthTransplantationModulationInflammationTransglutaminase activityExpressionCutaneousPathogenesisCancersNeonatal humanSupernatantsRegulationPsoriatic skinNormalToxicityCytotoxicityTransfectionCultures
HaCaT17
- These HaCaT cells also respond in vitro to external signals (e.g. levels of calcium, retinoids) similarly as normal keratinocytes. (springer.com)
- Thus, HaCaT and derived cell lines provide readily available cell culture models and might offer useful alternatives to primary or low-passaged normal keratinocytes. (springer.com)
- Intracellular protein carbonyl modification under CS, acrolein and acetaldehyde exposure in the HaCaT keratinocyte cell line, representing oral keratinocytes was examined by Western blot. (nih.gov)
- For this purpose, HaCaT human keratinocytes were exposed at 60.4 GHz with an incident power density of 20 mW/cm², this value corresponding to the upper local exposure limit for general public in the context of a wide scale deployment of MMW technologies and devices. (nature.com)
- The present study investigated cytotoxic effects of Fe-TAML using human keratinocytes (HaCaT). (cdc.gov)
- In relation, these authors examined CBD's protective effects against H 2 O 2 -induced oxidative stress in human keratinocyte HaCaT cells. (cosmeticsandtoiletries.com)
- In human keratinocytes (HEKa and HaCaT) treated with albendazole, cell cycle and proliferation, keratins and cell cycle-associated factors were evaluated by flow cytometry, colorimetric assay and Western blotting respectively. (portlandpress.com)
- Accordingly, human HaCaT keratinocytes were irradiated with a single dose of 20 Gy. (irsn.fr)
- To investigate the biological effects of MA in human skin keratinocytes, we investigated the potential cytotoxicity and apoptotic effects of MA in human keratinocyte cell lines (HaCaT). (mdpi.com)
- The data showed that MA induced apoptosis based on the observations of DAPI staining, DNA fragmentation, and sub-G1 phase in HaCaT cells and normal human epidermal keratinocytes (NHEKs). (mdpi.com)
- Considering the skin contact as one of the most feasible exposure routes to GBNs, the mechanism of toxicity of two GBNs (few-layer-graphene, FLG, and graphene oxide, GO) towards human HaCaT skin keratinocytes was investigated. (rsc.org)
- In this view, we recently demonstrated the ability of a few layer graphene (FLG) and different GOs to induce a sustained cytotoxicity related to a reduced mitochondrial activity in human skin HaCaT keratinocytes, a preliminary in vitro model to investigate skin toxicity. (rsc.org)
- The data confirmed that EECF greatly protected human HaCaT keratinocytes from PM 2.5 -induced oxidative stress. (medsci.org)
- Despite the altered and unlimited growth potential, HaCaT cells, similar to normal keratinocytes, reform an orderly structured and differentiated epidermal tissue when transplanted onto nude mice. (rupress.org)
- The characteristics of the HaCaT cell line clearly document that spontaneous transformation of human adult keratinocytes can occur in vitro and is associated with sequential chromosomal alterations, though not obligatorily linked to major defects in differentiation. (rupress.org)
- It was shown that LRRC8A is essential for VRAC activity and also contributes to regulatory volume decrease of HaCaT cells and primary keratinocytes. (logos-verlag.de)
- We found that DsrA was a keratinocyte-specific adhesin as it was necessary and sufficient for attachment to HaCaT cells, a keratinocyte cell line, but was not required for attachment to HS27 cells, a fibroblast cell line. (asm.org)
Differentiation26
- Keratinization is part of the physical barrier formation (cornification), in which the keratinocytes produce more and more keratin and undergo terminal differentiation. (wikipedia.org)
- During this differentiation process, keratinocytes permanently withdraw from the cell cycle, initiate expression of epidermal differentiation markers, and move suprabasally as they become part of the stratum spinosum, stratum granulosum, and eventually corneocytes in the stratum corneum. (wikipedia.org)
- Corneocytes are keratinocytes that have completed their differentiation program and have lost their nucleus and cytoplasmic organelles. (wikipedia.org)
- Factors promoting keratinocyte differentiation are: A calcium gradient, with the lowest concentration in the stratum basale and increasing concentrations until the outer stratum granulosum, where it reaches its maximum. (wikipedia.org)
- Vitamin D3 (cholecalciferol) regulates keratinocyte proliferation and differentiation mostly by modulating calcium concentrations and regulating the expression of genes involved in keratinocyte differentiation. (wikipedia.org)
- Since keratinocyte differentiation inhibits keratinocyte proliferation, factors that promote keratinocyte proliferation should be considered as preventing differentiation. (wikipedia.org)
- Keratinocyte transglutaminase enzymes serve to specifically catalyze the development of the cornified cell envelope, a defining characteristic of epidermal keratinocytes that have undergone the termination of differentiation. (wikipedia.org)
- In the past, immortalization of human epidermal keratinocytes has been generally considered to have a massive impact on their differentiation properties. (springer.com)
- Adenosine triphosphate binding cassette (ABC) transporters are expressed and regulated during terminal keratinocyte differentiation: a potential role for ABCA7 in epidermal lipid reorganization. (labome.org)
- Our results suggest specific biologic functions for members of the ABC transporter family in epidermal lipid reorganization during terminal keratinocyte differentiation. (labome.org)
- CLIC4 is multifunctional, traffics between the cytoplasm and nucleus, and participates in cell cycle control and differentiation in keratinocytes and other cell types. (aacrjournals.org)
- EGCG elicited cell differentiation with associated induction of p57/KIP2 within 24 h in growing keratinocytes, measured by the expression of keratin 1, filaggrin, and transglutaminase activity. (aspetjournals.org)
- MyD88 −/− or IL-1R −/− keratinocytes expressing oncogenic RAS are hyperproliferative and fail to up-regulate proinflammatory genes or down-regulate differentiation markers characteristic of RAS-expressing WT keratinocytes. (pubmedcentralcanada.ca)
- Using both genetic and pharmacological approaches, we find that the differentiation and proinflammatory effects of oncogenic RAS in keratinocytes require the establishment of an autocrine loop through IL-1α, IL-1R, and MyD88 leading to phosphorylation of IκBα and NF-κB activation. (pubmedcentralcanada.ca)
- Blocking IL-1α-mediated NF-κB activation in RAS-expressing WT keratinocytes reverses the differentiation defect and inhibits proinflammatory gene expression. (pubmedcentralcanada.ca)
- GR(EKO) mice treated with a low dose of 12-dimethylbenz(a) anthracene (DMBA) followed by phorbol 12-myristate 13-acetate (PMA) promotion exhibited earlier papilloma formation with higher incidence and multiplicity relative to control littermates (CO). Augmented proliferation and inflammation and defective differentiation of GR(EKO) keratinocytes contributed to the phenotype, likely through increased AKT and STAT3 (signal transducer and activator of transcription 3) activities. (csic.es)
- A previous report based on the cellular response to NAC treatment showed that NAC induced a 10-fold more rapid differentiation in normal primary keratinocytes as well as a reversion of a colon carcinoma cell line from neoplastic proliferation to apical-basolateral differentiation. (diva-portal.org)
- In order to investigate molecular events underlying the changes in proliferation and differentiation induced by NAC treatment, we performed global gene expression analysis of normal human epidermal keratinocytes in a time series. (diva-portal.org)
- Psoriasis vulgaris is a common chronic inflammatory skin disease characterized by the hyperproliferation and abnormal differentiation of keratinocytes. (spandidos-publications.com)
- Psoriasis vulgaris is a chronic inflammatory disorder characterized by the hyperproliferation and abnormal differentiation of keratinocytes and the infiltration of inflammatory cells into the dermis and epidermis. (spandidos-publications.com)
- therefore, C/EBPα may regulate the proliferation and differentiation of keratinocytes ( 8 , 9 ). (spandidos-publications.com)
- When keratinocytes were isolated from neonatal mouse skin and separated by Percoll density-gradient centrifugation according to their stage of differentiation, PGHS-1 mRNA expression and protein were found to be highest in the differentiated cells compared with those from the proliferative compartment. (portlandpress.com)
- The human epidermis is constantly self-renewing by proliferation and differentiation of keratinocytes and forms an important barrier, which is frequently challenged by various environmental influences including osmotic perturbations. (logos-verlag.de)
- Here, a CRISPR-Cas9-mediated LRRC8A gene knock-out was generated in human keratinocytes to investigate the function of LRRC8A during hypotonic stress response and differentiation. (logos-verlag.de)
- Taken together, LRRC8A is important in hypotonic stress response as well as keratinocyte proliferation and differentiation. (logos-verlag.de)
- It can be speculated that LRRC8A is another regulator for the transition from keratinocyte proliferation to differentiation and therefore important for epidermal homeostasis. (logos-verlag.de)
Epidermis21
- Keratinocytes are the primary type of cell found in the epidermis, the outermost layer of the skin. (wikipedia.org)
- Pathogens invading the upper layers of the epidermis can cause keratinocytes to produce proinflammatory mediators, particularly chemokines such as CXCL10 and CCL2 (MCP-1) which attract monocytes, natural killer cells, T-lymphocytes, and dendritic cells to the site of pathogen invasion. (wikipedia.org)
- Within the epidermis keratinocytes are associated with other cell types such as melanocytes and Langerhans cells. (wikipedia.org)
- Keratinocytes form tight junctions with the nerves of the skin and hold the Langerhans cells and intra-dermal lymphocytes in position within the epidermis. (wikipedia.org)
- found that contact with differentiated keratinocytes in the upper epidermis triggered vertical invasion by melanoma cells. (sciencemag.org)
- The dynamic interplay between keratinocytes and melanoma cells is further shown by an altered growth pattern of melanoma cells and the finding that a reconstructed epidermis induces invasion. (biomedsearch.com)
- Keratinocyte is the predominant cell type in the epidermis, the outermost layer of the skin. (scielo.br)
- Keratinocyte stem cells are found in the basal layer of the epidermis, which is the innermost layer of the epithelium, and has a basic proliferative function. (scielo.br)
- Within the epidermis, the nociceptive nerve fibers are surrounded by, and are in contact with, the epidermal keratinocytes. (cosmeticsandtoiletries.com)
- Interestingly, these nerve fibers are unmyelinated in the epidermis (C-fibers are unmyelinated while Ad-fibers lose their myelination when they enter the epidermis), allowing them to come into direct contact with the epidermal keratinocytes. (cosmeticsandtoiletries.com)
- As epidermis is continuously exposed to UV irradiation, we hypothesized that Cer hydrolysis protects keratinocytes from UVB-induced apoptosis by attenuating Cer levels. (biomedsearch.com)
- The keratinocyte is the major cell type of the epidermis, making up about 90% of epidermal cells. (genlantis.com)
- Primary Human Keratinocytes isolated from the epidermis of juvenile foreskin or adult skin from single or pooled donors. (promocell.com)
- Primary Normal Human Epidermal Keratinocytes (NHEK) are available from single or from pooled donors isolated from the epidermis of juvenile foreskin or adult skin from different locations like the face, the breasts, the abdomen, and the thighs. (promocell.com)
- Epidermal keratinocytes originate in the stratum basale and move up through the layers of the epidermis. (promocell.com)
- Serum-free cell culture medium for keratinocytes from the epidermis. (promocell.com)
- Our Primary Human Keratinocyte Cell Cultures are optimized to synthesize keratinocyte cells, the predominant cell type in the epidermis. (thermofisher.com)
- Characteristic histologic features of the disease include epidermal intercellular edema (spongiosis), keratinocyte damage, and the presence of an infiltrate of T cells, monocytes, and dendritic cells in the dermis and epidermis. (jimmunol.org)
- A KGF-immunoglobulin G fusion protein (KGF-HFc), which specifically and sensitively detects the KGFR, localized the receptor to differentiating keratinocytes of control epidermis, but revealed a striking decrease in receptor protein expression during the intermediate period of reepithelization. (rupress.org)
- CCATT/enhancer binding protein α (C/EBPα) is abundant in the epidermis and is associated with the proliferation of keratinocytes. (spandidos-publications.com)
- Transfer the suspension to a sterile 10 ml Falcon tube, and pump up and down to release keratinocytes from the epidermis. (openwetware.org)
Oxidative5
- To characterize oxidative stress in phospholipids of normal human epidermal keratinocytes we metabolically labeled their membrane phospholipids with a natural oxidation-sensitive fluorescent fatty acid, cis-parinaric acid, and exposed the cells to two different sources of oxidants--a lipid-soluble azo-initiator of peroxyl radicals, 2,2'-azobis(2,4-dimethyl-valeronitrile), AMVN, and a superoxide generator, xanthine oxidase/xanthine. (cdc.gov)
- Since viability of normal human epidermal keratinocytes was not changed either by labeling or exposure to oxidants the labeling protocol and oxidative stress employed are compatible with the quantitative analysis of phospholipid peroxidation in viable cells. (cdc.gov)
- α,β-Unsaturated aldehydes from CS are capable of intracellular protein carbonylation and have a role in intracellular oxidative stress elevation in keratinocytes, probably due to the reduction in GSH levels. (nih.gov)
- Green chemistry catalyst causes depletion of GSH, oxidative stress and cytotoxicity in keratinocytes in the presence of H2O2. (cdc.gov)
- Hyperproliferation of keratinocytes in psoriasis requires oxidative phosphorylation, in which the reduced form of nicotinamide adenine dinucleotide (NADH) is an electron donor. (termedia.pl)
Cells61
- with a group of neighbouring keratinocytes (keratin-synthesizing epidermal cells) into which its dendrites transfer pigment. (britannica.com)
- contains layers of cells called keratinocytes. (britannica.com)
- Basal cells in the basal layer (stratum basale) of the skin are sometimes referred to as basal keratinocytes. (wikipedia.org)
- The fully cornified keratinocytes that form the outermost layer are constantly shed off and replaced by new cells. (wikipedia.org)
- In humans, it is estimated that keratinocytes turn over from stem cells to desquamation every 40-56 days, whereas in mice the estimated turnover time is 8-10 days. (wikipedia.org)
- Keratinocytes are the only cells in the body with the entire vitamin D metabolic pathway from vitamin D production to catabolism and vitamin D receptor expression. (wikipedia.org)
- These factors include: The transcription factor p63, which prevents epidermal stem cells from differentiating into keratinocytes. (wikipedia.org)
- We now describe a new T cell antigen receptor (TCR) transgenic (Tg) model of inflammatory skin disease in which keratinocytes activate and are the primary target of autoreactive CD4 + T cells. (pnas.org)
- Thus, cutaneous immunopathology can be directed through antigen presentation by tissue-resident keratinocytes to autoreactive TCR Tg CD4 + cells. (pnas.org)
- Interestingly, the mice spontaneously develop autoimmune skin disease in which keratinocytes activate and are the primary targets of autoreactive CD4 + T cells. (pnas.org)
- Here we show that the p63 transcription factor, a p53 homologue essential for regenerative proliferation in epithelial development, distinguishes human keratinocyte stem cells from their TA progeny. (pnas.org)
- Human keratinocyte stem and TA cells when isolated in culture give rise to holoclones and paraclones, respectively. (pnas.org)
- A population of keratinocyte stem cells in defined locations governs the renewal of mammalian stratified epithelia ( 1 - 3 ). (pnas.org)
- In vivo , keratinocyte stem cells are usually slow-cycling and retain labeled DNA precursors, whereas TA cells divide rapidly and dilute their label quickly ( 4 - 8 ). (pnas.org)
- Epidermal keratinocytes were obtained from skin biopsies of healthy donors and cultivated on a feeder-layer of lethally irradiated 3T3-J2 cells as described ( 16 ). (pnas.org)
- Noninvasive melanoma cells became invasive in Matrigel when cocultured with differentiated keratinocytes but not with basal keratinocytes or fibroblasts, and transfection with an mCherry Notch reporter revealed that coculture with keratinocytes induced Notch signaling in melanoma cells. (sciencemag.org)
- Compared with mice injected with melanoma cells and either basal keratinocytes or fibroblasts, mice injected with melanoma cells and differentiated keratinocytes had greater numbers of lung metastases. (sciencemag.org)
- Sequencing of the small RNAs in melanoma cells cultured on DLL1-coated plates or cocultured with keratinocytes revealed that Notch signaling induced the expression of the miR-222 and miR-221 ( miR-222/221 ) cluster, the expression of which was observed only in invasive melanoma samples. (sciencemag.org)
- Although it has been previously assumed that glutamate receptors exist predominantly in the nervous system, growing evidence in recent years has suggested that they also exist in nonneuronal cells, such as keratinocytes and melanocytes [ 1 , 5 , 11 - 17 ]. (hindawi.com)
- The identification and purification of keratinocyte stem cells (KSCs) that are capable of self-renewal and maintenance of differentiating cell populations could contribute both to our understanding of the biology of these cells, and to significant clinical applications, such as the culturing of keratinocytes for transplantation to severe burn wounds. (ingentaconnect.com)
- Here, we report the detection of CD90 + cells in cultured normal human epidermal keratinocytes and adult skin. (ingentaconnect.com)
- Purified CD90 + keratinocytes demonstrated a sixfold higher cell growth rate than CD90 − cells and the ability to form large (over 3 mm in diameter) colonies. (ingentaconnect.com)
- In the reconstructed skin model, matrix metalloproteinase-9, a protease activated early in melanoma development, is secreted by the keratinocytes and subsequently activated by an unknown soluble factor secreted by the melanoma cells. (biomedsearch.com)
- Overall, our findings show that the invasive behavior of melanoma cells is determined by the melanoma cells themselves, but that the interplay between surrounding keratinocytes and the melanoma cells plays an important role in melanoma invasion. (biomedsearch.com)
- To investigate the use Aldefluor ® and N, N - Dimethylaminobenzaldehyde (DEAB) to design a protocol to sort keratinocyte stem cells from cultured keratinocytes from burned patients. (scielo.br)
- Flow cytometer range for keratinocyte stem cells separation was evaluated. (scielo.br)
- There were 14.8% of stem cells separated in one sample of keratinocyte culture used to pattern the protocol. (scielo.br)
- Aldefluor ® has been shown as a favorable marking of epidermal keratinocyte stem cells for subsequent separation on a flow cytometer, with detection of 10.8% of epidermal keratinocyte stem cells, in this protocol. (scielo.br)
- When keratinocyte culture is performed, the cells that adhere to the flask proliferate to form large colonies that at confluence fuse to form a sheet of keratinocytes 3 . (scielo.br)
- The objectives of the current study were to examine the effect of aldehydes originating from CS on intracellular proteins of oral keratinocytes and to observe the antioxidant response in these cells. (nih.gov)
- Keratinocyte proliferation and keratin (K) 6 and K16 expression were evaluated by immunohistochemistry and Western blotting and inflammatory cells/mediators were analysed by immunohistochemistry and real-time PCR. (portlandpress.com)
- Treatment of keratinocytes with albendazole reduced K6/K16 expression and reversibly inhibited cell growth by promoting accumulation of cells in S-phase. (portlandpress.com)
- Given our recent discovery that it is possible to separate human epidermal stem cells of the skin from their more committed progeny (i.e., transit-amplifying cells and early differentiating cells) using FACS techniques, we sought to determine the comparative tissue regeneration ability of these keratinocyte progenitors. (jci.org)
- Early differentiating keratinocytes exhibited limited short-term tissue regeneration under specific experimental conditions in this assay, although significant improvement was obtained by manipulating microenvironmental factors, that is, coculture with minimally passaged dermal cells or exogenous supply of the ECM protein laminin-10/11. (jci.org)
- These data illustrate that significant proliferative and tissue-regenerative capacity resides not only in keratinocyte stem cells as expected, but also in their more committed progeny, including early differentiating cells. (jci.org)
- KSCs and TA cells regenerate epithelial tissue better than differentiating keratinocytes when cocultured with p7 dermal cells. (jci.org)
- Note the relatively poor tissue-regenerative ability of the differentiating α 6 dim cells ( j ), which was further decreased by reducing the number of keratinocytes plated to 10 4 ( l ). (jci.org)
- A confocal image of dividing keratinocyte cells labelled with mitotracker (red), tubulin (green) and nuclei (Dapi-blue). (cellimagelibrary.org)
- Cells were part of a study of the effect of Papilloma viruses in keratinocytes. (cellimagelibrary.org)
- CFSE-stained keratinocytes and CFSE-stained melanocytes were transplanted to human full thickness in vitro wounds either as cell suspension for keratinocytes, or with the aid of macroporous gelatin microcarriers for both cells types in single and co-culture. (diva-portal.org)
- Viability and migration of CFSE-stained keratinocytes and melanocytes were investigated, and proliferation of the cells cultured on microcarriers was measured with flow cytometry. (diva-portal.org)
- The CFSE-staining of keratinocytes and melanocytes did not affect the viability, migration or proliferation of the cells. (diva-portal.org)
- The extracellular matrix provides a structural context for the resident cells and the epidermal keratinocytes, and a functioning interplay between the two tissue compartments is crucial for successful wound healing to take place. (diva-portal.org)
- Therefore, we examined the in vitro modulation of the production and activity of the components of the PA/plasmin system in squamous carcinoma cells (SCC-4) and normal human keratinocytes in relation to cell density and the presence or absence of fibroblasts (3T3 cells). (wur.nl)
- There was an inverse relation between cell density and mRNA expression for urokinase-type plasminogen activator (u-PA) and u-PA receptor in both SCC-4 cells and keratinocytes. (wur.nl)
- In contrast to monocultures, variation of cell density did not affect the mRNA expression of the components of the PA/plasmin system in cocultures of SCC-4 cells or keratinocytes with 3T3 cells. (wur.nl)
- The results of the present study reveal that the production and synthesis of various components of the PA/plasmin system in keratinocytes and SCC-4 cells depend on the density of epithelial cells and are modulated by fibroblasts, probably through a direct cell-cell or cell-matrix contact. (wur.nl)
- Fibroblast-induced modulations are similar in keratinocytes and SCC-4 cells except for the regulation of PAI-1, which is markedly enhanced only in keratinocytes. (wur.nl)
- Type I interferon (IFN)-response signatures in tubular cells and keratinocytes distinguished patients with LN from healthy control subjects. (nature.com)
- HEKa cells grown in EpiLife ( M-EPI-500-CA ) with the addition of Human Keratinocyte Growth Supplement ( HKGS ). (thermofisher.com)
- Addgene: Efficient and rapid generation of induced pluripotent stem cells from human keratinocytes. (addgene.org)
- Here we studied the capacity of distinct subsets of hapten-specific T cells to induce apoptosis in autologous keratinocytes. (jimmunol.org)
- Both Tc1 and Tc2 clones, but not CD4 + T cells, displayed a significant cytotoxic activity against resting nickel-modified keratinocytes. (jimmunol.org)
- Early ultrastructural studies of ACD revealed damaged keratinocytes in close contact to mononuclear cells ( 5 ), suggesting a role for T cell-mediated cytotoxicity in the expression of the disease, in line with other investigations on murine contact hypersensitivity (CH) ( 6 , 7 ). (jimmunol.org)
- PromoCell Keratinocyte Growth Medium 2 is a serum-free medium developed for the cultivation of epidermal keratinocytes without feeder cells. (promocell.com)
- Although all PromoCell media are optimized for use with primary human cells, we have received feedback from customers that this particular medium can also be used for horse and porcine keratinocytes, as well as mouse and rat keratinocytes after adapting the CaCl2 concentration. (promocell.com)
- Ideal for cells including NIKS (near-diploid immortalised keratinocytes) and primary keratinocytes, Trans IT-Keratinocyte is suitable for both stable and transient transfections. (bioscience.co.uk)
- A high DMBA dose followed by PMA promotion generated sebaceous adenomas and melanocytic foci in GR(EKO) and CO. Importantly, the number, growth kinetics, and extent of both tumor types increased in GR(EKO) mice, suggesting that in addition to regulating tumorigenesis from epidermal lineages, GR in keratinocytes is important for cross-talk with other skin cells. (csic.es)
- In contrast to mouse epidermal cells, human skin keratinocytes are rather resistant to transformation in vitro. (rupress.org)
- Supernatant from peripheral blood mononuclear cells (PBMCs) that had been exposed to the causative drug from patients with SJS/TEN induced the death of SJS/TEN keratinocytes, whereas supernatant from PBMCs of patients with ordinary drug skin reactions (ODSRs) exposed to the same drug did not. (sciencemag.org)
- A) The percent viability of infected keratinocytes was significantly higher in cells that were preexposed to L. rhamnosus GG (LGG+SA), lysate (LGG LYS+SA), or spent culture fluid (LGG CM+ SA) than that of S. aureus (SA)-infected cells. (asm.org)
Melanocytes4
- Burn treatment and conditions of hypopigmentation may require autologous transplantation of keratinocytes and melanocytes. (diva-portal.org)
- Sections from wounds with transplanted co-cultured keratinocytes and melanocytes were stained for pancytokeratin to distinguish double stained keratinocytes. (diva-portal.org)
- We propose a novel application of CFSE-staining in transplantation studies here presented with primary human keratinocytes and melanocytes. (diva-portal.org)
- Beta-carotene uptake and bioconversion to retinol differ between human melanocytes and keratinocytes. (diva-portal.org)
Mouse keratinocytes3
- Loss of RNF31 in mouse keratinocytes results in severe skin inflammation. (jimmunol.org)
- DIGITAL.CSIC: Selective ablation of glucocorticoid receptor in mouse keratinocytes increases susceptibility to skin tumorigenesis. (csic.es)
- Culture mouse keratinocytes at 33-34°C, 8% CO 2 for five to seven days before use in experiments. (openwetware.org)
Protects keratinocytes1
- Blockade of sphingosine kinase 1 (SPHK1) (but not SPHK2) by siRNA also increased apoptosis in L-UVB keratinocytes, revealing that conversion of sphingosine to sphingosine-1-phosphate (S1P) further protects keratinocytes from UVB-induced cell death. (biomedsearch.com)
Vitro7
- The human papillomavirus types (HPVs) most often associated with cancer of the cervix, such as HPV16, have been reported previously to immortalize normal human foreskin keratinocytes in vitro, while the types that are primarily associated with benign cervical lesions failed to do so. (nih.gov)
- Therefore, we conclude that HPV16 E6 and E7 cooperative to immortalize human keratinocytes in vitro. (nih.gov)
- CD90 + and CD90 − keratinocytes were purified from adult skin and cultured keratinocytes using fluorescent activated cell sorting, and their biological abilities were analysed using both in vitro and in vivo assays. (ingentaconnect.com)
- Immunocytochemical experiments revealed the unexpected presence of immunoreactivity for the M-type potassium channel subunit Kv7.2 in the keratinocyte layer of intact rat paw skin and in keratinocytes isolated from the skin of 1-day-old rats and cultured in vitro for 3-10 days. (sigmaaldrich.com)
- We examined the in vitro effects of 17beta-estradiol on RANTES production by human keratinocytes. (nih.gov)
- Work included in this thesis has applied viable human full thickness skin in vitro to investigate the re-epithelialisation process and barrier function of intact skin.The use of full thickness skin in vitro can take into account the contextual aspect of the process where the epidermal keratinocytes are activated and obtain a migratory phenotype, and are continuously dependent on the cues from the extracellular matrix and support of the dermis. (diva-portal.org)
- As an in vitro model system, patient-derived epidermolysis bullosa simplex keratinocytes have had an immense impact on what we know today about keratin filament function and their role in disease development. (diva-portal.org)
Cell22
- TA keratinocytes, immediately after their withdrawal from the stem cell compartment (meroclones), have greatly reduced p63, even though they possess very appreciable proliferative capacity. (pnas.org)
- Keratinocytes whose 14-3-3σ has been down-regulated remain in the stem cell compartment and maintain p63 during serial cultivation. (pnas.org)
- The identification of p63 as a keratinocyte stem cell marker will be of practical importance for the clinical application of epithelial cultures in cell therapy as well as for studies on epithelial tumorigenesis. (pnas.org)
- The specific cross linkages formed by keratinocyte transglutaminase are between n^ε-(γ-glutamyl)lysine residues which develop into isopeptide protein-protein linkages that adds to the stabilization of the cornified cell envelope. (wikipedia.org)
- cgb at biomail.ucsd.edu (Cindy Gustafson-Brown) wrote: I am interested in generating primary keratinocyte cell cultures and would appreciate any advice, references, protocols, and sources of reagents. (bio.net)
- Glutamate concentrations in cell supernatants of cultured keratinocytes increased proportionally to the cell density. (hindawi.com)
- 5 These proteins form channels in the plasma membrane that can react to thermal, mechanical and chemical stimuli and when activated, they allow for the transient entry of calcium into the cell in keratinocytes or membrane depolarization in sensory neurons. (cosmeticsandtoiletries.com)
- 6 In keratinocytes, this influx of calcium via activation of the TRPs activates a signaling cascade within the cell to promote the release of a variety of neuroactive substances, including: ATP, calcitonin gene-related peptide (CGRP), epinephrine, acetylcholine and cytokines such as IL-8, IL-1B and PGE2. (cosmeticsandtoiletries.com)
- Aim: To investigate whether albendazole, an anti-helmintic drug that regulates epithelial cell function in various systems, inhibits keratinocyte proliferation in models of psoriasis. (portlandpress.com)
- C ) Total cell number of survival primary keratinocytes that isolated from skin of 1-d-old WT and RNF31 E-KO mice ( n = 8 per group). (jimmunol.org)
- Keratinocytes in eight nodular basal cell carcinomas, one morpheic basal cell carcinoma, and one squamous cell carcinoma demonstrated intense membranous immunoperoxidase staining with mAb A1.5. (rupress.org)
- For most of the components of the PA/plasmin system, a decrease in mRNA expression and u-PA receptor protein was observed at most cell densities, whereas for PAI-1 only in keratinocytes a marked increase was documented. (wur.nl)
- Objectives: To assess the incidence and multiplicity of keratinocyte cancers (basal cell carcinoma [BCC] and squamous cell carcinoma [SCC]) excised in Australia, and to examine variations by age, sex, state, and prior skin cancer history. (apo.org.au)
- Normal Human Epidermal Keratinocyte Cell Culture in phase contrast. (promocell.com)
- Despite both being keratinocyte carcinomas mainly caused by ultraviolet radiation, cutaneous squamous cell carcinomas (SCCs) and basal cell carcinomas (BCCs) have different clinical behaviors. (ssrn.com)
- Collectively, these results demonstrate that MyD88 exerts a cell-intrinsic function in RAS-mediated transformation of keratinocytes. (pubmedcentralcanada.ca)
- Keratinocyte cancers are classified as either squamous cell carcinomas (SCC) or basal cell carcinomas (BCC) . (dermnetnz.org)
- Using fluorescence microscopy, we found that persistent SA, surviving intracellularly in keratinocytes, caused activation of the complement system with formation of the terminal complement complex (TCC) at the cell surface. (frontiersin.org)
- Normal keratinization in a spontaneously immortalized aneuploid human keratinocyte cell line. (rupress.org)
- Chronic exposure to tobacco resulted in increased cell scattering and invasiveness in immortalized oral keratinocytes. (amrita.edu)
- Stevens-Johnson syndrome (SJS) and toxic epidermal necrolysis (TEN) are life-threatening, cutaneous adverse drug reactions that are accompanied by keratinocyte cell death. (sciencemag.org)
- A similar distribution of PGHS-1 mRNA was found in keratinocytes from adult mice, whereas PGHS-1 protein was equally distributed in all cell types. (portlandpress.com)
Humans1
- Keratinocyte cancer (KC) is the most common form of cancer in humans. (onmedica.com)
Apoptosis in epidermal2
- Although ceramides (Cers) are key constituents of the epidermal permeability barrier, they also function as apoptogenic signals for UVB irradiation-induced apoptosis in epidermal keratinocytes. (biomedsearch.com)
- Im, Michelle, "ILK modulates stress-induced apoptosis in epidermal keratinocytes" (2014). (uwo.ca)
Mice10
- Interestingly, a significant number of mice that express both the K14-A β b transgene and the autoreactive 2-2-3 TCR spontaneously develop inflammatory skin disease with mononuclear infiltrates, induction of MHC class II expression on keratinocytes, and T helper 1 cytokines. (pnas.org)
- Enhanced green fluorescent protein (EGFP)-labelled CD90 + or CD90 − keratinocytes were subcutaneously injected into nonobese diabetic/severe combined immunodeficient (NOD/SCID) mice. (ingentaconnect.com)
- Results: Aldara-treated mice given albendazole exhibited reduced epidermal thickness, decreased number of proliferating keratinocytes and K6/K16 expression. (portlandpress.com)
- C ) Western blotting detection of the expression level of RNF31 in lysates of control and RNF31 E-KO primary keratinocytes from 1-d-old littermate mice (mouse genotyping from B). ( D ) Representative macroscopic images of 4-d-old WT and RNF31 E-KO littermate mice. (jimmunol.org)
- B ) The brief flow diagram of isolating primary keratinocytes from 1-d-old mice. (jimmunol.org)
- D ) Western blotting analysis of the indicated proteins in lysates of WT and RNF31 KO primary keratinocytes from 1-d-old littermate mice treated with TNF-α (20 ng/ml) and CHX (10 μg/ml). (jimmunol.org)
- F ) RT-PCR results of WT and RNF31 KO primary keratinocytes isolated from 1-d-old littermate mice treated with TNF-α (20 ng/ml) for 6 h. (jimmunol.org)
- TNFR1 deficiency in keratinocytes prevents skin inflammation in RNF31 E-KO mice. (jimmunol.org)
- Here, we generated mice with conditional deletion of TTP in keratinocytes (Zfp36fl/flK14-Cre mice, referred to herein as Zfp36ΔEP mice). (jci.org)
- Isolation of epidermal keratinocytes from neonatal mice is based on the protocol of Dlugosz et al. (openwetware.org)
Cytokines5
- However, activated keratinocytes produce inflammatory cytokines, including tumor necrosis factor α (TNF-α) and IL-1 ( 5 - 7 ) and clearly express both MHC class II molecules and the intercellular adhesion molecule-1 (ICAM-1). (pnas.org)
- Bacterial ghosts (BGs) as non-living bacterial envelopes devoid of cytoplasmic content with preserved and intact inner and outer membrane structures of their living counterparts have been used to study the ability of their surface components for the induction of antimicrobial peptides and pro-inflammatory cytokines in human primary keratinocytes (KCs). (nih.gov)
- Keratinocytes are also able to produce a variety of cytokines, growth factors, interleukins and complement factors. (promocell.com)
- Gene silencing of STAT2 by siRNA in human keratinocytes revealed that upon IFNα stimulation CXCL11 and CCL5 were the only two cytokines, among 102 analyzed, found to be regulated through a STAT2-dependent mechanism. (sigmaaldrich.com)
- Hematopoietic, lymphopoietic, and proinflammatory cytokines produced by human and murine keratinocytes. (harvard.edu)
Epithelial2
- The great proliferative potential of holoclones ( 9 - 12 ), the capacity of a single holoclone to generate a mature epithelium in vivo ( 13 ) and to differentiate into distinct cellular lineages ( 11 ), and the permanent epithelial regeneration achieved in burn victims by means of grafts of autologous cultured keratinocytes ( 14 - 16 ), provide compelling evidence that keratinocyte "stem-ness" can be preserved in culture. (pnas.org)
- Cultured GR(EKO) keratinocytes were spindle like, with loss of E-cadherin and upregulation of smooth muscle actin (SMA) and Snail, suggesting partial epithelial-mesenchymal transition. (csic.es)
Isolated cultured1
- Application of the M-channel enhancer retigabine (3-10 μM) to isolated cultured rat keratinocytes: (a) increased outward membrane currents recorded under voltage clamp, (b) produced ~3 mV hyperpolarization at rest, (c) enhanced ~3-fold the release of ATP induced by the TRPV3 agonist carvacrol (1 mM) and (d) increased the amplitude of the carvacrol-induced intracellular Ca(2+) transient measured with Fura-2. (sigmaaldrich.com)
Inhibition3
- Susanne Brodesser and Thomas Kolter, "Dihydroceramide Desaturase Inhibition by a Cyclopropanated Dihydroceramide Analog in Cultured Keratinocytes," Journal of Lipids , vol. 2011, Article ID 724015, 9 pages, 2011. (hindawi.com)
- Inhibition of CDase using either a CDase inhibitor, N-oleoylethanolamine, or small interfering RNA (siRNA) (either to a- and/or n-CDase(s)) sensitized keratinocytes to L-UVB-induced apoptosis in parallel with further Cer accumulation. (biomedsearch.com)
- All the data for Therapeutic inhibition of keratinocyte TRPV3 sensory channel by local anesthetic dyclonine have been deposited in Dyrad with DOI https://doi.org/10.5061/dryad.7d7wm37sq. (elifesciences.org)
Fibroblasts4
- Wölfle U, Heinemann A, Esser P, Haarhaus B, Martin S, Schempp C. Luteolin prevents solar radiation-induced matrix metalloproteinase-1 activation in human fibroblasts: a role for p38 mitogen-activated protein kinase and interleukin-20 released from keratinocytes. (labome.org)
- Our results suggest that SSR-mediated production of soluble factors in keratinocytes is modulated by luteolin and may attenuate photoaging in dermal fibroblasts. (labome.org)
- The progression from haemostasis and inflammation to proliferation of epidermal keratinocytes and dermal fibroblasts, and final scar maturation can be halted and result in a chronic wound that fails to re-epithelialise. (diva-portal.org)
- Our laboratory has previously identified that an environmentally relevant dose of UVA is able to induce the effect in human keratinocytes and fibroblasts, seen as reduced clonogenic survival. (lancs.ac.uk)
Oral keratinocytes2
- Cigarette smoke and chewing tobacco alter expression of different sets of miRNAs in oral keratinocytes. (amrita.edu)
- We developed cellular models of chronic exposure to chewing tobacco and cigarette smoke using immortalized oral keratinocytes. (amrita.edu)
Proteins5
- HPV16 E6 and E7 proteins cooperate to immortalize human foreskin keratinocytes. (nih.gov)
- As it turns out, epidermal keratinocytes express a broad range of sensory proteins. (cosmeticsandtoiletries.com)
- Both epidermal keratinocytes and epidermal nociceptive sensory nerves use a common family of proteins to detect noxious stimuli. (cosmeticsandtoiletries.com)
- Recently, we reported that PM 2.5 induced cellular macromolecule disruption of lipids, proteins, and DNA, via reactive oxygen species, eventually causing cellular apoptosis of human keratinocytes. (medsci.org)
- The complexity of bacterium-host interactions was further illustrated when various M proteins were expressed in an isogenic group A streptococcal background and their adherence to transformed human keratinocytes was compared ( 8 ). (asm.org)
Psoriasis6
- Conclusions: Data show that albendazole inhibits keratinocyte proliferation and exerts therapeutic effect in a murine model of psoriasis. (portlandpress.com)
- TGF-alpha expression in keratinocytes from normal individuals, patients with psoriasis, and patients with malignant skin diseases was investigated using an mAb raised against synthetic human TGF-alpha. (rupress.org)
- Keratinocytes in plaques from 18 psoriasis patients were more intensely stained than those from normal skin. (rupress.org)
- STAT2 is involved in the pathogenesis of psoriasis by promoting CXCL11 and CCL5 production by keratinocytes. (sigmaaldrich.com)
- In summary, these findings expand current views on the initiation of psoriasis and related arthritis by revealing the keratinocyte-intrinsic role of TTP. (jci.org)
- However, the role of C/EBPα in the proliferation of keratinocytes and the pathogenesis of psoriasis vulgaris are yet to be elucidated. (spandidos-publications.com)
Lesions3
- The production of RANTES is enhanced in keratinocytes of psoriatic skin lesions, which may contribute to the inflammatory infiltrate. (nih.gov)
- In this study, we detected abundant cytosolic DNA and increased AIM2 expression in keratinocytes in psoriatic lesions but not in healthy skin. (sciencemag.org)
- Dead keratinocytes from SJS/TEN lesions exhibited necrosis, by morphological criteria. (sciencemag.org)
Skin and cultured1
- The aim of this study was to assess the effect of YKS on skin and cultured human keratinocytes. (hindawi.com)
Primary keratinocyte2
Corneal keratinocytes2
- Limbal/corneal keratinocytes were obtained from ocular biopsies taken from organ donors and cultivated as described ( 11 ). (pnas.org)
- In addition, it is suited for rat corneal keratinocytes. (promocell.com)
Growth12
- The keratinocyte growth factor ( KGF ), also known as FGF7 , is a growth factor present in the epithelialization -phase of wound healing . (wikipedia.org)
- By itself, E6 exhibited no activity, Co-transfection of a plasmid with an intact E6 ORF and a second plasmid with an intact E7 ORF generated keratinocyte lines with indefinite growth potential. (nih.gov)
- We add 10 ng/mL KGF (from Epoch Biolabs) in keratinocyte growth medium that help a lot. (protocol-online.org)
- Werner S, Breeden M, Hübner G, Greenhalgh D, Longaker M. Induction of keratinocyte growth factor expression is reduced and delayed during wound healing in the genetically diabetic mouse. (labome.org)
- KC was originally identified by overexpression in murine keratinocytes, monocytes, and macrophages following stimulation by platelet-derived growth factor (PDGF) and macrophage colony-stimulating factor (M-CSF). (clontech.com)
- Canguilhem B, Pradines A, Baudouin C, Boby C, Lajoie-Mazenc I, Charveron M, Favre G (2005) RhoB protects human keratinocytes from UVB-induced apoptosis through epidermal growth factor receptor signaling. (springer.com)
- Detection of transforming growth factor alpha in normal, malignant, and hyperproliferative human keratinocytes. (rupress.org)
- The demonstration of TGF-alpha in normal keratinocytes suggests that it plays a role in normal keratinocyte growth, wound healing, and in the pathogenesis of acanthosis. (rupress.org)
- Aged keratinocytes, which exhibited low basal cellular activities after culturing in growth medium for up to 25 days, renewed DNA synthesis and activated succinate dehydrogenase up to 37-fold upon exposure to either EGCG or the polyphenols. (aspetjournals.org)
- Modulation of keratinocyte growth factor and its receptor in reepithelializing human skin. (rupress.org)
- We investigated the expression and distribution of keratinocyte growth factor (KGF) (FGF-7) and its receptor (KGFR) during reepithelialization of human skin. (rupress.org)
- We now show that ILK-deficient keratinocytes exhibit lower proliferative capacity and increased apoptosis in the absence or presence of growth factors. (uwo.ca)
Transplantation3
- Importantly, transplantation of all classes of keratinocyte progenitors into an in vivo setting demonstrated that tissue regeneration can be elicited from stem, transit-amplifying, and early differentiating keratinocytes for up to 10 weeks. (jci.org)
- Outcomes of autologous non-cultured melanocyte keratinocyte transplantation in vitiligo and nevus depigmentosus. (medworm.com)
- In particular, noncultured melanocyte-keratinocyte transplantation (MKTP) has recently gained popularit y because it can treat a large area at once.2 However, this procedure does not always achieve complete repigmentation after a single session.3 If the remaining lesion is small and scattered, it may not be practical to repeatedly apply noncultured MKTP, considering the time and effort required for th e surgery. (medworm.com)
Modulation1
- We conclude that (i) bacterial M protein and keratinocyte CD46 do not mediate adherence of M49 skin-associated Streptococcus pyogenes to epidermal keratinocytes, (ii) hyaluronic acid capsule impedes the interaction of bacterial adhesins with keratinocyte receptors, (iii) modulation of capsule expression may be important in the pathogenesis of skin infections, and (iv) the molecular interactions in attachment of skin strains of S. pyogenes to keratinocytes are unique and remain unidentified. (asm.org)
Inflammation3
- RNF31 deficiency in keratinocytes triggers skin inflammation. (jimmunol.org)
- Therefore keratinocytes are important for wound healing, inflammation, and immune response. (promocell.com)
- The multimodal sensory channel transient receptor potential vanilloid-3 (TRPV3) is expressed in epidermal keratinocytes and implicated in chronic pruritus, allergy, and inflammation-related skin disorders. (elifesciences.org)
Transglutaminase activity1
- A study on the mutation of keratinocyte transglutaminase (TGK) came to conclude that those affected with ichthyosis lamellaris, present a substantial deficit in keratinocyte transglutaminase activity. (wikipedia.org)
Expression9
- Changes in cellular gene expression are probably also required for immortalization since all of the keratinocyte lines examined were aneuploid. (nih.gov)
- The mRNA expression levels of NMDA receptor 2D (NMDAR2D) and glutamate aspartate transporter (GLAST) were also determined in YKS-stimulated cultured keratinocytes. (hindawi.com)
- However, during expansion of the culture, the expression level of CD90 rapidly decreased to about 2·5% at passage 10, while most of the keratinocytes maintained expression of α 6 integrin. (ingentaconnect.com)
- 17beta-estradiol inhibited tumor necrosis factor-alpha or interleukin-1beta-induced RANTES secretion, mRNA expression, and promoter activity in keratinocytes, and these effects of 17beta-estradiol were counteracted by estrogen receptor antagonist ICI 182 780. (nih.gov)
- Expression of α 6 integrin ( b , e , h , and k ) in the cultures corresponding to a , d , g , and j revealed that an integrin-positive basal layer (most polarized in the KSC sheet) was present in the epithelium generated from all fractions as well as total UF keratinocytes. (jci.org)
- Elevated levels of CLIC4 in primary keratinocytes lead to the expression of a truncated form of Smad7 (Smad7Δ), which is detectable as a PCR product. (aacrjournals.org)
- Gene expression of carbonyl-metabolizing enzymes (CMEs) was investigated in normal buccal keratinocytes (NBK) and the transformed buccal keratinocyte lines SVpgC2a and SqCC/Y1. (diva-portal.org)
- reported UVB irradiation-induced secretion of TNF-α from keratinocytes, which could be inhibited by tacrolimus by downregulation of nuclear factor kappa B (NF-κB) expression. (frontiersin.org)
- None of the mutants, including the M-protein-deficient ( emm mutant) strain, displayed reduced adherence to early-passage cultured human keratinocytes, but adherence of the mutant lacking hyaluronic acid capsule expression ( has mutant) was increased 13-fold. (asm.org)
Cutaneous2
- These characteristics suggest that keratinocytes may directly participate in cutaneous immunologic diseases. (pnas.org)
- Adams S, Valchanova R, Munz B. RIP2: a novel player in the regulation of keratinocyte proliferation and cutaneous wound repair? (labome.org)
Pathogenesis2
- Background: Increased keratinocyte proliferation occurs in the skin of psoriatic patients and is supposed to play a role in the pathogenesis of this disorder. (portlandpress.com)
- Finally, we wanted to examine the importance of keratinocyte adhesion in chancroid pathogenesis so we tested the wild-type and dsrA mutant strains of H. ducreyi in our swine models of chancroid pathogenesis. (asm.org)
Cancers4
- There is strong evidence for a role of environmental risk factors involved in susceptibility to develop multiple keratinocyte cancers (mKCs), but whether genes are also involved in mKCs susceptibility has not been thoroughly investigated. (harvard.edu)
- What are keratinocyte cancers? (dermnetnz.org)
- Keratinocyte cancers are considered low risk or high risk, depending on patient factors such as age and immune suppression status, and tumour factors including tumour site and histological subtype. (dermnetnz.org)
- What are the high-risk features of keratinocyte cancers? (dermnetnz.org)
Neonatal human1
- Normal neonatal human keratinocytes (HEKn) ( C-001-5C ) were grown in EpiLife medium and in a keratinocyte medium from a leading competitor. (thermofisher.com)
Supernatants1
- Zymography of supernatants revealed that the levels of both free u-PA and PA-PAI were increased in SCC-4/3T3 co-cultures, whereas in keratinocyte/3T3 co-cultures, only levels of the PA-PAI complex were increased, while the amount of free u-PA activity decreased. (wur.nl)
Regulation2
- We conclude that Rac1 is functionally required for normal epidermal wound healing and, in this context, exerts a dual function - namely the regulation of keratinocyte proliferation and migration. (labome.org)
- Topographical regulation of keratinocyte migration. (cellimagelibrary.org)
Psoriatic skin1
- Moreover, the antimicrobial cathelicidin peptide LL-37, which can interact with DNA in psoriatic skin, neutralized cytosolic DNA in keratinocytes and blocked AIM2 inflammasome activation. (sciencemag.org)
Normal4
- keratinocytes are nonfunctional, tolerizing APCs in normal skin. (pnas.org)
- We demonstrated that both oxidants induced pronounced oxidation of four major classes of cis-parinaric acid-labeled phospholipids-phosphatidylcholine, phosphatidylethanolamine, phosphatidylserine, and phosphatidylinositol-in normal human epidermal keratinocytes that was not detectable as any significant change of their phospholipid composition. (cdc.gov)
- Cultured normal keratinocytes demonstrated membranous staining with mAb A1.5. (rupress.org)
- Our findings underline a distinct, novel role for ILK in promoting keratinocyte survival and a normal redox state. (uwo.ca)
Toxicity1
- Trans IT®-Keratinocyte from Mirus Bio is a high efficiency, low toxicity, plasmid DNA transfection reagent, specifically optimised for keratinocyte transfection. (bioscience.co.uk)
Cytotoxicity1
- Following IFN-γ treatment, keratinocytes expressed MHC class II and ICAM-1 and became susceptible to Th1-mediated, but not Th2-mediated, cytotoxicity. (jimmunol.org)
Transfection1
- Transfection with a plasmid in which E6 and E7 were the only intact open reading frames (ORFs) induced an indefinite life-span in the keratinocytes with an efficiency similar to that of the entire early region of the viral DNA. (nih.gov)
Cultures2
- Phototoxic effects of Hypericum extract in cultures of human keratinocytes compared with those of psoralen. (labome.org)
- Robust HPV-18 production in organotypic cultures of primary human keratinocytes. (nextbio.com)