A CELL LINE derived from human T-CELL LEUKEMIA and used to determine the mechanism of differential susceptibility to anti-cancer drugs and radiation.
Lymphocytes responsible for cell-mediated immunity. Two types have been identified - cytotoxic (T-LYMPHOCYTES, CYTOTOXIC) and helper T-lymphocytes (T-LYMPHOCYTES, HELPER-INDUCER). They are formed when lymphocytes circulate through the THYMUS GLAND and differentiate to thymocytes. When exposed to an antigen, they divide rapidly and produce large numbers of new T cells sensitized to that antigen.
A tumor necrosis factor receptor subtype found in a variety of tissues and on activated LYMPHOCYTES. It has specificity for FAS LIGAND and plays a role in regulation of peripheral immune responses and APOPTOSIS. Multiple isoforms of the protein exist due to multiple ALTERNATIVE SPLICING. The activated receptor signals via a conserved death domain that associates with specific TNF RECEPTOR-ASSOCIATED FACTORS in the CYTOPLASM.
A malignant disease of the T-LYMPHOCYTES in the bone marrow, thymus, and/or blood.
One of the mechanisms by which CELL DEATH occurs (compare with NECROSIS and AUTOPHAGOCYTOSIS). Apoptosis is the mechanism responsible for the physiological deletion of cells and appears to be intrinsically programmed. It is characterized by distinctive morphologic changes in the nucleus and cytoplasm, chromatin cleavage at regularly spaced sites, and the endonucleolytic cleavage of genomic DNA; (DNA FRAGMENTATION); at internucleosomal sites. This mode of cell death serves as a balance to mitosis in regulating the size of animal tissues and in mediating pathologic processes associated with tumor growth.
A family of intracellular CYSTEINE ENDOPEPTIDASES that play a role in regulating INFLAMMATION and APOPTOSIS. They specifically cleave peptides at a CYSTEINE amino acid that follows an ASPARTIC ACID residue. Caspases are activated by proteolytic cleavage of a precursor form to yield large and small subunits that form the enzyme. Since the cleavage site within precursors matches the specificity of caspases, sequential activation of precursors by activated caspases can occur.
A transmembrane protein belonging to the tumor necrosis factor superfamily that was originally discovered on cells of the lymphoid-myeloid lineage, including activated T-LYMPHOCYTES and NATURAL KILLER CELLS. It plays an important role in immune homeostasis and cell-mediated toxicity by binding to the FAS RECEPTOR and triggering APOPTOSIS.
A short pro-domain caspase that plays an effector role in APOPTOSIS. It is activated by INITIATOR CASPASES such as CASPASE 9. Isoforms of this protein exist due to multiple alternative splicing of its MESSENGER RNA.
Established cell cultures that have the potential to propagate indefinitely.
Molecules on the surface of T-lymphocytes that recognize and combine with antigens. The receptors are non-covalently associated with a complex of several polypeptides collectively called CD3 antigens (ANTIGENS, CD3). Recognition of foreign antigen and the major histocompatibility complex is accomplished by a single heterodimeric antigen-receptor structure, composed of either alpha-beta (RECEPTORS, ANTIGEN, T-CELL, ALPHA-BETA) or gamma-delta (RECEPTORS, ANTIGEN, T-CELL, GAMMA-DELTA) chains.
A soluble substance elaborated by antigen- or mitogen-stimulated T-LYMPHOCYTES which induces DNA synthesis in naive lymphocytes.
A long pro-domain caspase that contains a death effector domain in its pro-domain region. Caspase 8 plays a role in APOPTOSIS by cleaving and activating EFFECTOR CASPASES. Activation of this enzyme can occur via the interaction of its N-terminal death effector domain with DEATH DOMAIN RECEPTOR SIGNALING ADAPTOR PROTEINS.
This enzyme is a lymphoid-specific src family tyrosine kinase that is critical for T-cell development and activation. Lck is associated with the cytoplasmic domains of CD4, CD8 and the beta-chain of the IL-2 receptor, and is thought to be involved in the earliest steps of TCR-mediated T-cell activation.
The uptake of naked or purified DNA by CELLS, usually meaning the process as it occurs in eukaryotic cells. It is analogous to bacterial transformation (TRANSFORMATION, BACTERIAL) and both are routinely employed in GENE TRANSFER TECHNIQUES.
A long pro-domain caspase that contains a caspase recruitment domain in its pro-domain region. Caspase 9 is activated during cell stress by mitochondria-derived proapoptotic factors and by CARD SIGNALING ADAPTOR PROTEINS such as APOPTOTIC PROTEASE-ACTIVATING FACTOR 1. It activates APOPTOSIS by cleaving and activating EFFECTOR CASPASES.
The intracellular transfer of information (biological activation/inhibition) through a signal pathway. In each signal transduction system, an activation/inhibition signal from a biologically active molecule (hormone, neurotransmitter) is mediated via the coupling of a receptor/enzyme to a second messenger system or to an ion channel. Signal transduction plays an important role in activating cellular functions, cell differentiation, and cell proliferation. Examples of signal transduction systems are the GAMMA-AMINOBUTYRIC ACID-postsynaptic receptor-calcium ion channel system, the receptor-mediated T-cell activation pathway, and the receptor-mediated activation of phospholipases. Those coupled to membrane depolarization or intracellular release of calcium include the receptor-mediated activation of cytotoxic functions in granulocytes and the synaptic potentiation of protein kinase activation. Some signal transduction pathways may be part of larger signal transduction pathways; for example, protein kinase activation is part of the platelet activation signal pathway.
Cells grown in vitro from neoplastic tissue. If they can be established as a TUMOR CELL LINE, they can be propagated in cell culture indefinitely.
A phorbol ester found in CROTON OIL with very effective tumor promoting activity. It stimulates the synthesis of both DNA and RNA.
Morphologic alteration of small B LYMPHOCYTES or T LYMPHOCYTES in culture into large blast-like cells able to synthesize DNA and RNA and to divide mitotically. It is induced by INTERLEUKINS; MITOGENS such as PHYTOHEMAGGLUTININS, and by specific ANTIGENS. It may also occur in vivo as in GRAFT REJECTION.
A family of transcription factors characterized by the presence of highly conserved calcineurin- and DNA-binding domains. NFAT proteins are activated in the CYTOPLASM by the calcium-dependent phosphatase CALCINEURIN. They transduce calcium signals to the nucleus where they can interact with TRANSCRIPTION FACTOR AP-1 or NF-KAPPA B and initiate GENETIC TRANSCRIPTION of GENES involved in CELL DIFFERENTIATION and development. NFAT proteins stimulate T-CELL activation through the induction of IMMEDIATE-EARLY GENES such as INTERLEUKIN-2.
Conversion of an inactive form of an enzyme to one possessing metabolic activity. It includes 1, activation by ions (activators); 2, activation by cofactors (coenzymes); and 3, conversion of an enzyme precursor (proenzyme or zymogen) to an active enzyme.
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
Endogenous and exogenous compounds and that either inhibit CASPASES or prevent their activation.
Mucoproteins isolated from the kidney bean (Phaseolus vulgaris); some of them are mitogenic to lymphocytes, others agglutinate all or certain types of erythrocytes or lymphocytes. They are used mainly in the study of immune mechanisms and in cell culture.
Inhibitors of SERINE ENDOPEPTIDASES and sulfhydryl group-containing enzymes. They act as alkylating agents and are known to interfere in the translation process.
A signal-transducing adaptor protein that associates with TNF RECEPTOR complexes. It contains a death effector domain that can interact with death effector domains found on INITIATOR CASPASES such as CASPASE 8 and CASPASE 10. Activation of CASPASES via interaction with this protein plays a role in the signaling cascade that leads to APOPTOSIS.
Exogenous and endogenous compounds which inhibit CYSTEINE ENDOPEPTIDASES.
Technique using an instrument system for making, processing, and displaying one or more measurements on individual cells obtained from a cell suspension. Cells are usually stained with one or more fluorescent dyes specific to cell components of interest, e.g., DNA, and fluorescence of each cell is measured as it rapidly transverses the excitation beam (laser or mercury arc lamp). Fluorescence provides a quantitative measure of various biochemical and biophysical properties of the cell, as well as a basis for cell sorting. Other measurable optical parameters include light absorption and light scattering, the latter being applicable to the measurement of cell size, shape, density, granularity, and stain uptake.
Anti-CD3 monoclonal antibody that exerts immunosuppressive effects by inducing peripheral T-cell depletion and modulation of the T-cell receptor complex (CD3/Ti).
Complex of at least five membrane-bound polypeptides in mature T-lymphocytes that are non-covalently associated with one another and with the T-cell receptor (RECEPTORS, ANTIGEN, T-CELL). The CD3 complex includes the gamma, delta, epsilon, zeta, and eta chains (subunits). When antigen binds to the T-cell receptor, the CD3 complex transduces the activating signals to the cytoplasm of the T-cell. The CD3 gamma and delta chains (subunits) are separate from and not related to the gamma/delta chains of the T-cell receptor (RECEPTORS, ANTIGEN, T-CELL, GAMMA-DELTA).
Glycoproteins found on the membrane or surface of cells.
The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.
The introduction of a phosphoryl group into a compound through the formation of an ester bond between the compound and a phosphorus moiety.
Antigens expressed on the cell membrane of T-lymphocytes during differentiation, activation, and normal and neoplastic transformation. Their phenotypic characterization is important in differential diagnosis and studies of thymic ontogeny and T-cell function.
A sialic acid-rich protein and an integral cell membrane mucin. It plays an important role in activation of T-LYMPHOCYTES.
Glycoprotein members of the immunoglobulin superfamily which participate in T-cell adhesion and activation. They are expressed on most peripheral T-lymphocytes, natural killer cells, and thymocytes, and function as co-receptors or accessory molecules in the T-cell receptor complex.
A human cell line established from a diffuse histiocytic lymphoma (HISTIOCYTIC LYMPHOMA, DIFFUSE) and displaying many monocytic characteristics. It serves as an in vitro model for MONOCYTE and MACROPHAGE differentiation.
Compounds or agents that combine with an enzyme in such a manner as to prevent the normal substrate-enzyme combination and the catalytic reaction.
Ubiquitous, inducible, nuclear transcriptional activator that binds to enhancer elements in many different cell types and is activated by pathogenic stimuli. The NF-kappa B complex is a heterodimer composed of two DNA-binding subunits: NF-kappa B1 and relA.
A broad category of carrier proteins that play a role in SIGNAL TRANSDUCTION. They generally contain several modular domains, each of which having its own binding activity, and act by forming complexes with other intracellular-signaling molecules. Signal-transducing adaptor proteins lack enzyme activity, however their activity can be modulated by other signal-transducing enzymes
A promyelocytic cell line derived from a patient with ACUTE PROMYELOCYTIC LEUKEMIA. HL-60 cells lack specific markers for LYMPHOID CELLS but express surface receptors for FC FRAGMENTS and COMPLEMENT SYSTEM PROTEINS. They also exhibit phagocytic activity and responsiveness to chemotactic stimuli. (From Hay et al., American Type Culture Collection, 7th ed, pp127-8)
Semiautonomous, self-reproducing organelles that occur in the cytoplasm of all cells of most, but not all, eukaryotes. Each mitochondrion is surrounded by a double limiting membrane. The inner membrane is highly invaginated, and its projections are called cristae. Mitochondria are the sites of the reactions of oxidative phosphorylation, which result in the formation of ATP. They contain distinctive RIBOSOMES, transfer RNAs (RNA, TRANSFER); AMINO ACYL T RNA SYNTHETASES; and elongation and termination factors. Mitochondria depend upon genes within the nucleus of the cells in which they reside for many essential messenger RNAs (RNA, MESSENGER). Mitochondria are believed to have arisen from aerobic bacteria that established a symbiotic relationship with primitive protoeukaryotes. (King & Stansfield, A Dictionary of Genetics, 4th ed)
Membrane proteins encoded by the BCL-2 GENES and serving as potent inhibitors of cell death by APOPTOSIS. The proteins are found on mitochondrial, microsomal, and NUCLEAR MEMBRANE sites within many cell types. Overexpression of bcl-2 proteins, due to a translocation of the gene, is associated with follicular lymphoma.
Any of the processes by which nuclear, cytoplasmic, or intercellular factors influence the differential control (induction or repression) of gene action at the level of transcription or translation.
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
A basic element found in nearly all organized tissues. It is a member of the alkaline earth family of metals with the atomic symbol Ca, atomic number 20, and atomic weight 40. Calcium is the most abundant mineral in the body and combines with phosphorus to form calcium phosphate in the bones and teeth. It is essential for the normal functioning of nerves and muscles and plays a role in blood coagulation (as factor IV) and in many enzymatic processes.
Differentiation antigens residing on mammalian leukocytes. CD stands for cluster of differentiation, which refers to groups of monoclonal antibodies that show similar reactivity with certain subpopulations of antigens of a particular lineage or differentiation stage. The subpopulations of antigens are also known by the same CD designation.
RNA sequences that serve as templates for protein synthesis. Bacterial mRNAs are generally primary transcripts in that they do not require post-transcriptional processing. Eukaryotic mRNA is synthesized in the nucleus and must be exported to the cytoplasm for translation. Most eukaryotic mRNAs have a sequence of polyadenylic acid at the 3' end, referred to as the poly(A) tail. The function of this tail is not known for certain, but it may play a role in the export of mature mRNA from the nucleus as well as in helping stabilize some mRNA molecules by retarding their degradation in the cytoplasm.
Costimulatory T-LYMPHOCYTE receptors that have specificity for CD80 ANTIGEN and CD86 ANTIGEN. Activation of this receptor results in increased T-cell proliferation, cytokine production and promotion of T-cell survival.
Splitting the DNA into shorter pieces by endonucleolytic DNA CLEAVAGE at multiple sites. It includes the internucleosomal DNA fragmentation, which along with chromatin condensation, are considered to be the hallmarks of APOPTOSIS.
DNA sequences which are recognized (directly or indirectly) and bound by a DNA-dependent RNA polymerase during the initiation of transcription. Highly conserved sequences within the promoter include the Pribnow box in bacteria and the TATA BOX in eukaryotes.
A non-essential amino acid. In animals it is synthesized from PHENYLALANINE. It is also the precursor of EPINEPHRINE; THYROID HORMONES; and melanin.
Proteins which bind to DNA. The family includes proteins which bind to both double- and single-stranded DNA and also includes specific DNA binding proteins in serum which can be used as markers for malignant diseases.
The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.
Molecule composed of the non-covalent association of the T-cell antigen receptor (RECEPTORS, ANTIGEN, T-CELL) with the CD3 complex (ANTIGENS, CD3). This association is required for the surface expression and function of both components. The molecule consists of up to seven chains: either the alpha/beta or gamma/delta chains of the T-cell receptor, and four or five chains in the CD3 complex.
Derivatives of phosphatidic acids in which the phosphoric acid is bound in ester linkage to a serine moiety. Complete hydrolysis yields 1 mole of glycerol, phosphoric acid and serine and 2 moles of fatty acids.
Antibodies produced by a single clone of cells.
A progressive, malignant disease of the blood-forming organs, characterized by distorted proliferation and development of leukocytes and their precursors in the blood and bone marrow. Leukemias were originally termed acute or chronic based on life expectancy but now are classified according to cellular maturity. Acute leukemias consist of predominately immature cells; chronic leukemias are composed of more mature cells. (From The Merck Manual, 2006)
An ERYTHROLEUKEMIA cell line derived from a CHRONIC MYELOID LEUKEMIA patient in BLAST CRISIS.
The biosynthesis of RNA carried out on a template of DNA. The biosynthesis of DNA from an RNA template is called REVERSE TRANSCRIPTION.
Transport proteins that carry specific substances in the blood or across cell membranes.
A group of heterogeneous lymphoid tumors representing malignant transformations of T-lymphocytes.
The relationship between the dose of an administered drug and the response of the organism to the drug.
Products of proto-oncogenes. Normally they do not have oncogenic or transforming properties, but are involved in the regulation or differentiation of cell growth. They often have protein kinase activity.
The process in which substances, either endogenous or exogenous, bind to proteins, peptides, enzymes, protein precursors, or allied compounds. Specific protein-binding measures are often used as assays in diagnostic assessments.
A protein of the annexin family isolated from human PLACENTA and other tissues. It inhibits cytosolic PHOSPHOLIPASE A2, and displays anticoagulant activity.
Proteins found in the nucleus of a cell. Do not confuse with NUCLEOPROTEINS which are proteins conjugated with nucleic acids, that are not necessarily present in the nucleus.
Members of the class of neutral glycosphingolipids. They are the basic units of SPHINGOLIPIDS. They are sphingoids attached via their amino groups to a long chain fatty acyl group. They abnormally accumulate in FABRY DISEASE.
Endogenous substances, usually proteins, which are effective in the initiation, stimulation, or termination of the genetic transcription process.
Regulatory sequences important for viral replication that are located on each end of the HIV genome. The LTR includes the HIV ENHANCER, promoter, and other sequences. Specific regions in the LTR include the negative regulatory element (NRE), NF-kappa B binding sites , Sp1 binding sites, TATA BOX, and trans-acting responsive element (TAR). The binding of both cellular and viral proteins to these regions regulates HIV transcription.
The type species of LENTIVIRUS and the etiologic agent of AIDS. It is characterized by its cytopathic effect and affinity for the T4-lymphocyte.
White blood cells formed in the body's lymphoid tissue. The nucleus is round or ovoid with coarse, irregularly clumped chromatin while the cytoplasm is typically pale blue with azurophilic (if any) granules. Most lymphocytes can be classified as either T or B (with subpopulations of each), or NATURAL KILLER CELLS.
Glycoproteins with a wide distribution on hematopoietic and non-hematopoietic cells and strongly expressed on macrophages. CD58 mediates cell adhesion by binding to CD2; (ANTIGENS, CD2); and this enhances antigen-specific T-cell activation.
A negative regulatory effect on physiological processes at the molecular, cellular, or systemic level. At the molecular level, the major regulatory sites include membrane receptors, genes (GENE EXPRESSION REGULATION), mRNAs (RNA, MESSENGER), and proteins.
The span of viability of a cell characterized by the capacity to perform certain functions such as metabolism, growth, reproduction, some form of responsiveness, and adaptability.
A semisynthetic derivative of PODOPHYLLOTOXIN that exhibits antitumor activity. Etoposide inhibits DNA synthesis by forming a complex with topoisomerase II and DNA. This complex induces breaks in double stranded DNA and prevents repair by topoisomerase II binding. Accumulated breaks in DNA prevent entry into the mitotic phase of cell division, and lead to cell death. Etoposide acts primarily in the G2 and S phases of the cell cycle.
A member of the Bcl-2 protein family that reversibly binds MEMBRANES. It is a pro-apoptotic protein that is activated by caspase cleavage.
The parts of a macromolecule that directly participate in its specific combination with another molecule.
Aggressive T-Cell malignancy with adult onset, caused by HUMAN T-LYMPHOTROPIC VIRUS 1. It is endemic in Japan, the Caribbean basin, Southeastern United States, Hawaii, and parts of Central and South America and sub-Saharan Africa.
Recombinant proteins produced by the GENETIC TRANSLATION of fused genes formed by the combination of NUCLEIC ACID REGULATORY SEQUENCES of one or more genes with the protein coding sequences of one or more genes.
An indolocarbazole that is a potent PROTEIN KINASE C inhibitor which enhances cAMP-mediated responses in human neuroblastoma cells. (Biochem Biophys Res Commun 1995;214(3):1114-20)
Processes that stimulate the GENETIC TRANSCRIPTION of a gene or set of genes.
An eph family receptor found primarily in BRAIN and THYMUS. The EphB6 receptor is unusual in that its tyrosine kinase domain shares little homology with other members of this class. The unusual tyrosine kinase domain of this receptor appears to result in its lack of tyrosine kinase activity.
A superfamily of PROTEIN-SERINE-THREONINE KINASES that are activated by diverse stimuli via protein kinase cascades. They are the final components of the cascades, activated by phosphorylation by MITOGEN-ACTIVATED PROTEIN KINASE KINASES, which in turn are activated by mitogen-activated protein kinase kinase kinases (MAP KINASE KINASE KINASES).
The rate dynamics in chemical or physical systems.
The first continuously cultured human malignant CELL LINE, derived from the cervical carcinoma of Henrietta Lacks. These cells are used for VIRUS CULTIVATION and antitumor drug screening assays.
A divalent calcium ionophore that is widely used as a tool to investigate the role of intracellular calcium in cellular processes.
Proto-oncogene proteins that are guanine nucleotide exchange factors for RHO GTPASES. They also function as signal transducing adaptor proteins.
A protein tyrosine kinase that is required for T-CELL development and T-CELL ANTIGEN RECEPTOR function.
A strain of PRIMATE T-LYMPHOTROPIC VIRUS 1 isolated from mature T4 cells in patients with T-lymphoproliferation malignancies. It causes adult T-cell leukemia (LEUKEMIA-LYMPHOMA, T-CELL, ACUTE, HTLV-I-ASSOCIATED), T-cell lymphoma (LYMPHOMA, T-CELL), and is involved in mycosis fungoides, SEZARY SYNDROME and tropical spastic paraparesis (PARAPARESIS, TROPICAL SPASTIC).
The lipid- and protein-containing, selectively permeable membrane that surrounds the cytoplasm in prokaryotic and eukaryotic cells.
An alkaloid obtained from the betel nut (Areca catechu), fruit of a palm tree. It is an agonist at both muscarinic and nicotinic acetylcholine receptors. It is used in the form of various salts as a ganglionic stimulant, a parasympathomimetic, and a vermifuge, especially in veterinary practice. It has been used as a euphoriant in the Pacific Islands.
A large group of proteins that control APOPTOSIS. This family of proteins includes many ONCOGENE PROTEINS as well as a wide variety of classes of INTRACELLULAR SIGNALING PEPTIDES AND PROTEINS such as CASPASES.
A water-soluble, enzyme co-factor present in minute amounts in every living cell. It occurs mainly bound to proteins or polypeptides and is abundant in liver, kidney, pancreas, yeast, and milk.
An enzyme that catalyzes the acetylation of chloramphenicol to yield chloramphenicol 3-acetate. Since chloramphenicol 3-acetate does not bind to bacterial ribosomes and is not an inhibitor of peptidyltransferase, the enzyme is responsible for the naturally occurring chloramphenicol resistance in bacteria. The enzyme, for which variants are known, is found in both gram-negative and gram-positive bacteria. EC 2.3.1.28.
Proteins encoded by the TAT GENES of the HUMAN IMMUNODEFICIENCY VIRUS.
Cytochromes of the c type that are found in eukaryotic MITOCHONDRIA. They serve as redox intermediates that accept electrons from MITOCHONDRIAL ELECTRON TRANSPORT COMPLEX III and transfer them to MITOCHONDRIAL ELECTRON TRANSPORT COMPLEX IV.
Transcriptional trans-acting proteins of the promoter elements found in the long terminal repeats (LTR) of HUMAN T-LYMPHOTROPIC VIRUS 1 and HUMAN T-LYMPHOTROPIC VIRUS 2. The tax (trans-activator x; x is undefined) proteins act by binding to enhancer elements in the LTR.
An serine-threonine protein kinase that requires the presence of physiological concentrations of CALCIUM and membrane PHOSPHOLIPIDS. The additional presence of DIACYLGLYCEROLS markedly increases its sensitivity to both calcium and phospholipids. The sensitivity of the enzyme can also be increased by PHORBOL ESTERS and it is believed that protein kinase C is the receptor protein of tumor-promoting phorbol esters.
A subgroup of mitogen-activated protein kinases that activate TRANSCRIPTION FACTOR AP-1 via the phosphorylation of C-JUN PROTEINS. They are components of intracellular signaling pathways that regulate CELL PROLIFERATION; APOPTOSIS; and CELL DIFFERENTIATION.
Proteins which are found in membranes including cellular and intracellular membranes. They consist of two types, peripheral and integral proteins. They include most membrane-associated enzymes, antigenic proteins, transport proteins, and drug, hormone, and lectin receptors.
Proteins prepared by recombinant DNA technology.
A sesquiterpene lactone found in roots of THAPSIA. It inhibits CA(2+)-TRANSPORTING ATPASE mediated uptake of CALCIUM into SARCOPLASMIC RETICULUM.
Trans-acting transcription factors produced by retroviruses such as HIV. They are nuclear proteins whose expression is required for viral replication. The tat protein stimulates LONG TERMINAL REPEAT-driven RNA synthesis for both viral regulatory and viral structural proteins. tat stands for trans-activation of transcription.
A long pro-domain caspase that contains a caspase recruitment domain in its pro-domain region. Activation of this enzyme can occur via the interaction of its caspase recruitment domain with CARD SIGNALING ADAPTOR PROTEINS. Caspase 2 plays a role in APOPTOSIS by cleaving and activating effector pro-caspases. Several isoforms of this protein exist due to multiple alternative splicing of its MESSENGER RNA.
A group of cytochromes with covalent thioether linkages between either or both of the vinyl side chains of protoheme and the protein. (Enzyme Nomenclature, 1992, p539)
A multiprotein complex composed of the products of c-jun and c-fos proto-oncogenes. These proteins must dimerize in order to bind to the AP-1 recognition site, also known as the TPA-responsive element (TRE). AP-1 controls both basal and inducible transcription of several genes.
A class of compounds composed of repeating 5-carbon units of HEMITERPENES.
Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others.
A plant genus of the family CELASTRACEAE that is a source of triterpenoids and diterpene epoxides such as triptolide.
The fluid inside CELLS.
The termination of the cell's ability to carry out vital functions such as metabolism, growth, reproduction, responsiveness, and adaptability.
A phosphoinositide phospholipase C subtype that is primarily regulated by PROTEIN-TYROSINE KINASES. It is structurally related to PHOSPHOLIPASE C DELTA with the addition of SRC HOMOLOGY DOMAINS and pleckstrin homology domains located between two halves of the CATALYTIC DOMAIN.
Serum glycoprotein produced by activated MACROPHAGES and other mammalian MONONUCLEAR LEUKOCYTES. It has necrotizing activity against tumor cell lines and increases ability to reject tumor transplants. Also known as TNF-alpha, it is only 30% homologous to TNF-beta (LYMPHOTOXIN), but they share TNF RECEPTORS.
A critical subpopulation of T-lymphocytes involved in the induction of most immunological functions. The HIV virus has selective tropism for the T4 cell which expresses the CD4 phenotypic marker, a receptor for HIV. In fact, the key element in the profound immunosuppression seen in HIV infection is the depletion of this subset of T-lymphocytes.
Within a eukaryotic cell, a membrane-limited body which contains chromosomes and one or more nucleoli (CELL NUCLEOLUS). The nuclear membrane consists of a double unit-type membrane which is perforated by a number of pores; the outermost membrane is continuous with the ENDOPLASMIC RETICULUM. A cell may contain more than one nucleus. (From Singleton & Sainsbury, Dictionary of Microbiology and Molecular Biology, 2d ed)

The hematopoietic-specific adaptor protein gads functions in T-cell signaling via interactions with the SLP-76 and LAT adaptors. (1/6443)

BACKGROUND: The adaptor protein Gads is a Grb2-related protein originally identified on the basis of its interaction with the tyrosine-phosphorylated form of the docking protein Shc. Gads protein expression is restricted to hematopoietic tissues and cell lines. Gads contains a Src homology 2 (SH2) domain, which has previously been shown to have a similar binding specificity to that of Grb2. Gads also possesses two SH3 domains, but these have a distinct binding specificity to those of Grb2, as Gads does not bind to known Grb2 SH3 domain targets. Here, we investigated whether Gads is involved in T-cell signaling. RESULTS: We found that Gads is highly expressed in T cells and that the SLP-76 adaptor protein is a major Gads-associated protein in vivo. The constitutive interaction between Gads and SLP-76 was mediated by the carboxy-terminal SH3 domain of Gads and a 20 amino-acid proline-rich region in SLP-76. Gads also coimmunoprecipitated the tyrosine-phosphorylated form of the linker for activated T cells (LAT) adaptor protein following cross-linking of the T-cell receptor; this interaction was mediated by the Gads SH2 domain. Overexpression of Gads and SLP-76 resulted in a synergistic augmentation of T-cell signaling, as measured by activation of nuclear factor of activated T cells (NFAT), and this cooperation required a functional Gads SH2 domain. CONCLUSIONS: These results demonstrate that Gads plays an important role in T-cell signaling via its association with SLP-76 and LAT. Gads may promote cross-talk between the LAT and SLP-76 signaling complexes, thereby coupling membrane-proximal events to downstream signaling pathways.  (+info)

Tyrosine phosphorylation and complex formation of Cbl-b upon T cell receptor stimulation. (2/6443)

Cbl-b, a mammalian homolog of Cbl, consists of an N-terminal region (Cbl-b-N) highly homologous to oncogenic v-Cbl, a Ring finger, and a C-terminal region containing multiple proline-rich stretches and potential tyrosine phosphorylation sites. In the present study, we demonstrate that upon engagement of the T cell receptor (TCR), endogenous Cbl-b becomes rapidly tyrosine-phosphorylated. In heterogeneous COS-1 cells, Cbl-b was phosphorylated on tyrosine residues by both Syk- (Syk/Zap-70) and Src- (Fyn/Lck) family kinases, with Syk kinase inducing the most prominent effect. Syk associates and phosphorylates Cbl-b in Jurkat T cells. A Tyr-316 Cbl-binding site in Syk was required for the association with and for the maximal tyrosine phosphorylation of Cbl-b. Mutation at a loss-of-function site (Gly-298) in Cbl-b-N disrupts its interaction with Syk. Cbl-b constitutively binds Grb2 and becomes associated with Crk-L upon TCR stimulation. The Grb2- and the Crk-L-binding regions were mapped to the C-terminus of Cbl-b. The Crk-L-binding sites were further determined to be Y655DVP and Y709KIP, with the latter being the primary binding site. Taken together, these results implicate that Cbl-b is involved in TCR-mediated intracellular signaling pathways.  (+info)

Reactive oxygen intermediate-dependent NF-kappaB activation by interleukin-1beta requires 5-lipoxygenase or NADPH oxidase activity. (3/6443)

We previously reported that the role of reactive oxygen intermediates (ROIs) in NF-kappaB activation by proinflammatory cytokines was cell specific. However, the sources for ROIs in various cell types are yet to be determined and might include 5-lipoxygenase (5-LOX) and NADPH oxidase. 5-LOX and 5-LOX activating protein (FLAP) are coexpressed in lymphoid cells but not in monocytic or epithelial cells. Stimulation of lymphoid cells with interleukin-1beta (IL-1beta) led to ROI production and NF-kappaB activation, which could both be blocked by antioxidants or FLAP inhibitors, confirming that 5-LOX was the source of ROIs and was required for NF-kappaB activation in these cells. IL-1beta stimulation of epithelial cells did not generate any ROIs and NF-kappaB induction was not influenced by 5-LOX inhibitors. However, reintroduction of a functional 5-LOX system in these cells allowed ROI production and 5-LOX-dependent NF-kappaB activation. In monocytic cells, IL-1beta treatment led to a production of ROIs which is independent of the 5-LOX enzyme but requires the NADPH oxidase activity. This pathway involves the Rac1 and Cdc42 GTPases, two enzymes which are not required for NF-kappaB activation by IL-1beta in epithelial cells. In conclusion, three different cell-specific pathways lead to NF-kappaB activation by IL-1beta: a pathway dependent on ROI production by 5-LOX in lymphoid cells, an ROI- and 5-LOX-independent pathway in epithelial cells, and a pathway requiring ROI production by NADPH oxidase in monocytic cells.  (+info)

Jun kinase phosphorylates and regulates the DNA binding activity of an octamer binding protein, T-cell factor beta1. (4/6443)

POU domain proteins have been implicated as key regulators during development and lymphocyte activation. The POU domain protein T-cell factor beta1 (TCFbeta1), which binds octamer and octamer-related sequences, is a potent transactivator. In this study, we showed that TCFbeta1 is phosphorylated following activation via the T-cell receptor or by stress-induced signals. Phosphorylation of TCFbeta1 occurred predominantly at serine and threonine residues. Signals which upregulate Jun kinase (JNK)/stress-activated protein kinase activity also lead to association of JNK with TCFbeta1. JNK associates with the activation domain of TCFbeta1 and phosphorylates its DNA binding domain. The phosphorylation of recombinant TCFbeta1 by recombinant JNK enhances the ability of TCFbeta1 to bind to a consensus octamer motif. Consistent with this conclusion, TCFbeta1 upregulates reporter gene transcription in an activation- and JNK-dependent manner. In addition, inhibition of JNK activity by catalytically inactive MEKK (in which methionine was substituted for the lysine at position 432) also inhibits the ability of TCFbeta1 to drive inducible transcription from the interleukin-2 promoter. These results suggest that stress-induced signals and T-cell activation induce JNK, which then acts on multiple cis sequences by modulating distinct transactivators like c-Jun and TCFbeta1. This demonstrates a coupling between the JNK activation pathway and POU domain proteins and implicates TCFbeta1 as a physiological target in the JNK signal transduction pathway leading to coordinated biological responses.  (+info)

Activation-dependent transcriptional regulation of the human Fas promoter requires NF-kappaB p50-p65 recruitment. (5/6443)

Fas (CD95) and Fas ligand (CD95L) are an interacting receptor-ligand pair required for immune homeostasis. Lymphocyte activation results in the upregulation of Fas expression and the acquisition of sensitivity to FasL-mediated apoptosis. Although Fas upregulation is central to the preservation of immunologic tolerance, little is known about the molecular machinery underlying this process. To investigate the events involved in activation-induced Fas upregulation, we have examined mRNA accumulation, fas promoter activity, and protein expression in the Jurkat T-cell line treated with phorbol myristate acetate and ionomycin (P/I), pharmacological mimics of T-cell receptor activation. Although resting Jurkat cells express Fas, Fas mRNA was induced approximately 10-fold in 2 h upon P/I stimulation. Using sequential deletion mutants of the human fas promoter in transient transfection assays, we identified a 47-bp sequence (positions -306 to -260 relative to the ATG) required for activation-driven fas upregulation. Sequence analysis revealed the presence of a previously unrecognized composite binding site for both the Sp1 and NF-kappaB transcription factors at positions -295 to -286. Electrophoretic mobility shift assay (EMSA) and supershift analyses of this region documented constitutive binding of Sp1 in unactivated nuclear extracts and inducible binding of p50-p65 NF-kappaB heterodimers after P/I activation. Sp1 and NF-kappaB transcription factor binding was shown to be mutually exclusive by EMSA displacement studies with purified recombinant Sp1 and recombinant p50. The functional contribution of the kappaB-Sp1 composite site in P/I-inducible fas promoter activation was verified by using kappaB-Sp1 concatamers (-295 to -286) in a thymidine kinase promoter-driven reporter construct and native promoter constructs in Jurkat cells overexpressing IkappaB-alpha. Site-directed mutagenesis of the critical guanine nucleotides in the kappaB-Sp1 element documented the essential role of this site in activation-dependent fas promoter induction.  (+info)

Requirement for transcription factor NFAT in interleukin-2 expression. (6/6443)

The nuclear factor of activated T cells (NFAT) transcription factor is implicated in expression of the cytokine interleukin-2 (IL-2). Binding sites for NFAT are located in the IL-2 promoter. Furthermore, pharmacological studies demonstrate that the drug cyclosporin A inhibits both NFAT activation and IL-2 expression. However, targeted disruption of the NFAT1 and NFAT2 genes in mice does not cause decreased IL-2 secretion. The role of NFAT in IL-2 gene expression is therefore unclear. Here we report the construction of a dominant-negative NFAT mutant (dnNFAT) that selectively inhibits NFAT-mediated gene expression. The inhibitory effect of dnNFAT is mediated by suppression of activation-induced nuclear translocation of NFAT. Expression of dnNFAT in cultured T cells caused inhibition of IL-2 promoter activity and decreased expression of IL-2 protein. Similarly, expression of dnNFAT in transgenic mice also caused decreased IL-2 gene expression. These data demonstrate that NFAT is a critical component of the signaling pathway that regulates IL-2 expression.  (+info)

Physical interaction of the bHLH LYL1 protein and NF-kappaB1 p105. (7/6443)

The LYL1 gene was first identified upon the molecular characterization of the t(7;9)(q35;p13) translocation associated with some human T-cell acute leukemias (T-ALLs). In adult tissues, LYL1 expression is restricted to hematopoietic cells with the notable exclusion of the T cell lineage. LYL1 encodes a basic helix-loop-helix (bHLH) protein highly related to TAL-1, whose activation is also associated with a high proportion of human T-ALLs. A yeast two-hybrid system was used to identify proteins that specifically interact with LYL1 and might mediate its activities. We found that p105, the precursor of NF-kappaB1 p50, was the major LYL1-interacting protein in this system. The association between LYL1 and p105 was confirmed both in vitro and in vivo in mammalian cells. Biochemical studies indicated that the interaction was mediated by the bHLH motif of LYL1 and the ankyrin-like motifs of p105. Ectopic expression of LYL1 in a human T cell line caused a significant decrease in NF-kappaB-dependent transcription, associated with a reduced level of NF-kappaB1 proteins.  (+info)

Proteolytic processing of the Alzheimer's disease amyloid precursor protein within its cytoplasmic domain by caspase-like proteases. (8/6443)

Alzheimer's disease is characterized by neurodegeneration and deposition of betaA4, a peptide that is proteolytically released from the amyloid precursor protein (APP). Missense mutations in the genes coding for APP and for the polytopic membrane proteins presenilin (PS) 1 and PS2 have been linked to familial forms of early-onset Alzheimer's disease. Overexpression of presenilins, especially that of PS2, induces increased susceptibility for apoptosis that is even more pronounced in cells expressing presenilin mutants. Additionally, presenilins themselves are targets for activated caspases in apoptotic cells. When we analyzed APP in COS-7 cells overexpressing PS2, we observed proteolytic processing close to the APP carboxyl terminus. Proteolytic conversion was increased in the presence of PS2-I, which encodes one of the known PS2 pathogenic mutations. The same proteolytic processing occurred in cells treated with chemical inducers of apoptosis, suggesting a participation of activated caspases in the carboxyl-terminal truncation of APP. This was confirmed by showing that specific caspase inhibitors blocked the apoptotic conversion of APP. Sequence analysis of the APP cytosolic domain revealed a consensus motif for group III caspases ((IVL)ExD). Mutation of the corresponding Asp664 residue abolished cleavage, thereby identifying APP as a target molecule for caspase-like proteases in the pathways of programmed cellular death.  (+info)

BioAssay record AID 1270344 submitted by ChEMBL: Induction of cell proliferation in human Jurkat T cells at 200 uM after 24 hrs by tryphan blue assay.
A growing body of evidence strongly indicates that both simulated and authentic weightlessness exert a broad range of effects on mammalian tissues and cells, including impairment of immune cell function and increased apoptotic death. We previously reported that microgravity-dependent activation of 5-lipoxygenase (5-LOX) might play a central role in the initiation of apoptosis in human T lymphocytes, suggesting that the upregulation of this enzyme might be (at least in part) responsible for immunodepression observed in astronauts during space flights. Herein, we supplement novel information about the molecular mechanisms underlying microgravity-triggered apoptotic cell death and immune system deregulation, demonstrating that under simulated microgravity human Jurkat T cells increase the content of cytosolic DNA fragments and cytochrome c (typical hallmarks of apoptosis) and have an upregulated expression and activity of |i|µ|/i|-calpain. These events were paralleled by the unbalance of interleukin-
Citation: Macho, A., Blanco-Molina, M., Spagliardi, P., Appendino, G., Bremner, P., Heinrich, M., Fiebich, B. L. and Munoz, E. (2004) Calcium ionophoretic and apoptotic effects of ferutinin in the human Jurkat T-cell line. Biochemical Pharmacology, 68 (5), pp. 875-883. ...
Citation : Macho, A., Blanco-Molina, M., Spagliardi, P., Appendino, G., Bremner, P., Heinrich, M., Fiebich, B. L. and Munoz, E. (2004) Calcium ionophoretic and apoptotic effects of ferutinin in the human Jurkat T-cell line. Biochemical Pharmacology, 68 (5), pp. 875-883. ...
BioAssay record AID 1077914 submitted by ChEMBL: Antiviral activity against HIV1 infected in human Jurkat cells assessed as inhibition of viral replication by CAT gene-based transcomplementation assay.
The Western diet is high in omega-6 fatty acids and low in omega-3 fatty acids. Canola oil contains a healthier omega 3 to omega 6 ratio than corn oil. Jurkat T leukemia cells were treated with free fatty acids mixtures in ratios mimicking that found in commercially available canola oil (7% α-linolenic, 30% linoleic, 54% oleic) or corn oil (59% linoleic, 24% oleic) to determine the cell survival or cell death and changes in expression levels of inflammatory cytokines and receptors following oil treatment. Fatty acid uptake was assessed by gas chromatography. Cell survival and cell death were evaluated by cell cycle analyses, propidium-iodide staining, trypan blue exclusion and phosphatidylserine externalization. mRNA levels of inflammatory cytokines and receptors were assessed by RT-PCR. There was a significant difference in the lipid profiles of the cells after treatment. Differential action of the oils on inflammatory molecules, following treatment at non-cytotoxic levels, indicated that canola oil
Apoptotic cells release the nucleotides ATP and UTP to attract phagocytic cells, which in turn clear the apoptotic cells. Chekeni et al. found that carbenoxolone (CBX), which inhibits connexins (which form gap junctions) and pannexins (which form plasma membrane channels), and probenicid, which is thought to be more specific for pannexins, reduced ATP release from apoptotic Jurkat cells to a similar extent as the caspase inhibitor zVAD (which blocks the release of ATP from apoptotic cells). Small interfering RNAs (siRNAs) directed against pannexin 1 (PANX1) decreased the release of ATP and UTP from apoptotic Jurkat cells but did not prevent apoptosis. Supernatant from PANX1 siRNA-transfected apoptotic cells recruited fewer monocytes in an in vitro assay of cell migration and when placed in a subcutaneous air pouch in mice. In contrast, Jurkat cells stably overexpressing PANX1 released more ATP and UTP, and supernatants from these cells (after apoptosis had been induced) recruited more monocytes ...
Certain clones of Jurkat cells are susceptible to necroptosis, but not all of them. In my experience, the FADD-deficient clone 5C3 is highly susceptible to necroptotic death induced by TNFa. The wild type jurkat cell line A3 is not and the caspase-8 deficient line I9.2 is only mildly susceptible by itself, but, surprisingly, becomes more susceptible upon addition of z-VAD-fmk, suggesting that caspase-8 is not essential to prevent necroptosis in these cells. The RIPK1-deficient jurkat cell line is not susceptible to necroptosis unless reconstituted with RIPK1 harbouring a cleavage site mutation, The parental clone doesnt undergo necroptosis upon TNFa stimulation in the presence of z-VAD-fmk. However, the RIPK1 deficient cells are extremely susceptible to all forms of apoptosis, suggesting either an important role of RIPK1 in preventing apoptosis or that these cells lack another anti-apoptotic factor in addition to RIPK1. These cells were initially generated by selecting randomly mutated jurkat ...
Grape pomace is a rich source of phenolic compounds commonly employed for elaboration of dietary supplements. The aim of the present study was to investigate the anticancer effect of a purified white grape pomace extract (PWGPE) in acute lymphoblastic leukemia Jurkat cells and to characterize the underlying
Creative Biolabs provides Anti-MCC peptide (ADLIAYLKQATK) T Cell Receptor (clone 226), Jurkat Cell Line product for Biopharmaceutical research,preclinical and clinical trials.
Creative Biolabs provides Anti-RAC1 (FSGEHPTV) T Cell Receptor (clone T3), Jurkat Cell Line product for Biopharmaceutical research,preclinical and clinical trials.
The Neo Jurkat cell line was derived by transfecting human Jurkat T cells with the empty pSFFV mammalian expression vector carrying a neomycin-resistant gene.  Stable neomycin (Neo)-resistant single-cell-originated clones were isolated and expanded by selection in medium containing 1 mg/mL G418 for 14 days.
ABSTRACT: RNA interference (RNAi) is a potent gene delivery system for studying the regulation of gene expression in a wide variety of eukaryotic cells. In the present study, different RNAi approaches, namely, synthetic small interfering RNA (siRNA) and plasmid- and lentivirus-based short hairpin RNA (shRNA) were investigated to assess the down-regulation of Nck1 protein efficiency in the Jurkat T cell line. Jurkat T cells treated with these three different systems substantially and specifically reduced the expression of the Nck1 protein but not that of the Nck2 protein. Although the three systems showed a similar Nck1 knockdown efficiency, they led to different T cell activation outcomes. After stimulation, CD69 expression and IL-2 production were impaired in Nck1-siRNA and plasmid-based Nck1-shRNA transfected Jurkat cells. However, these T cell activation outcomes were increased in lentiviral vector based Nck1-shRNA transfected cells. These data suggest that the outcomes from transfection with ...
Show moreBACKGROUND: Kallikrein 6 (KLK6) is a newly identified member of the kallikrein family of secreted serine proteases that prior studies indicate is elevated at sites of central nervous system (CNS) inflammation and which shows regulated expression with T cell activation. Notably, KLK6 is also elevated in the serum of multiple sclerosis (MS) patients however its potential roles in immune function are unknown. Herein we specifically examine whether KLK6 alters immune cell survival and the possible mechanism by which this may occur. METHODOLOGY/PRINCIPAL FINDINGS: Using murine whole splenocyte preparations and the human Jurkat T cell line we demonstrate that KLK6 robustly supports cell survival across a range of cell death paradigms. Recombinant KLK6 was shown to significantly reduce cell death under resting conditions and in response to camptothecin, dexamethasone, staurosporine and Fas-ligand. Moreover, KLK6-over expression in Jurkat T cells was shown to generate parallel pro-survival ...
CD2-mediated T lymphocyte activation requires surface expression of CD3-Ti, the T cell receptor (TCR) for antigen major histocompatibility complex protein. Given the importance of CD3 zeta in TCR signaling, we have directly examined the ability of the CD3 zeta cytoplasmic domain to couple CD2 to intracellular signal transduction pathways. A cDNA encoding a chimeric protein consisting of the human CD3 zeta cytoplasmic domain (amino acid residues 31-142) fused to the CD8 alpha extracellular and transmembrane domains (amino acid residues 1-187) was transfected into a CD2+CD3-CD8- variant of the human T cell line Jurkat. The resulting transfectants expressed the CD8 alpha/CD3 zeta chimeric receptor at the cell surface in the absence of other TCR subunits. Stimulation of these transfectants with anti-T11(2) + anti-T11(3) monoclonal antibodies (mAbs) initiated both a prompt cytosolic free calcium ([Ca2+]i) rise and protein tyrosine kinase activation. Stimulation with either intact anti-T11(2) + ...
Out of the entire population of cells, only the apoptotic Daudi cells immediately decreased CD19 expression via capping, while only the apoptotic Jurkat cells increased CD3 receptor expression 24 h post-exposure. Both receptor changes occurred in a UVA1 dose-dependent manner. We also examined other T-cell receptors, such as CD4, CD25, and CD69, but they did not change for up to 24 h following exposure. During UVA1-triggered immediate apoptosis of Jurkat T cells, IFN- levels increased in a dose-dependent manner at 4 h, but returned to baseline levels at 24 h post-exposure, whereas, there was no significant change in IL-2 at 4 or 24 h. Conclusion ...
Jurkat cell - posted in Tissue and Cell Culture: Hi How can I do to separate live and dead Jurkat cells.? I recently thawed this cell line. I counted with trypan blue and saw many dead cells. Please, help me !!! I do not want to lose these cells.
human Jurkat T cells were treated with increasing concentrations of PDTI and SBTI at different incubation times and the result was assessed employing a old-fashioned tetrazolium centered colorimetric cell proliferation assay. After 24 h incubation at 37 C, 25 cell viability was decreased by uM PDTI in a 30_4%. On another hand, SBTI had a impact, Celecoxib since at 25 uM attention it caused 45_6% cell stability diminution, and even at 2. 5 uM cell viability lowered in a 23_4%. Already after 6 h incubation, 25 uM SBTI caused significant reduction in cell viability, while PDTI expected longer incubation time to make a significant impact. After 24 h of culture, the reduction in cell viability was optimum for both trypsin inhibitors. Longer periods of incubation did not produce significant differences with respect to 24 h. For subsequent studies, designed to comprehend the mechanism through which these trypsin inhibitors decrease stability of Jurkat cells, the PDTI and SBTI levels picked Gene ...
Transmission electron micrograph of a Jurkat T-cell in the early stages of apoptosis, with apoptotic bodies forming. The Jurkat cell line is derived from human T-cell leukemia and used to determine the mechanism of differential susceptibility to anti-cancer drugs and radiation.
The Jurkat cell line was established from the peripheral blood of a 14 year old boy by Schneider et al., and was originally designated JM. The line was cloned from cells obtained from Dr. Kendall Smith and are mycoplasma free. This is a clone of the Jurkat-FHCRC cell line, a derivative of the Jurkat cell line.
The T cell surface molecule CD28 can provide costimulatory signals that permit the full activation of T cells. Here we demonstrate that stimulation of CD28, either by B7, its natural ligand, or by the anti-CD28 monoclonal antibody 9.3, induces an association between CD28 and phosphatidylinositol 3-kinase (PI3-K) in Jurkat T cells, raising the possibility that an interaction with PI3-K contributes to CD28-mediated signaling. To examine the mechanism of the association, we synthesized tyrosine-phosphorylated oligopeptides corresponding to each of the four tyrosines in the CD28 cytoplasmic domain. When added to lysates of B7-stimulated Jurkat cells, the oligopeptide corresponding to Tyr 173 inhibits the coimmunoprecipitation of PI3-K with CD28; the other oligopeptides have no effect. Tyr 173 is contained within the sequence YMNM, a motif that is also found in the platelet-derived growth factor receptor and that, when phosphorylated, forms a high affinity binding site for the p85 subunit of PI3-K. ...
Fig. 2 Functional analysis of TCRs encoded by peripheral blood TFH cells.. (A) Sorting and gating strategy for follicular helper CD4+ T cells. CD3+CD4+CXCR5+CD45RA− PD1++ TFH cells were isolated from PBMCs at day 7 post vaccination from a donor vaccinated with the Fluzone vaccine. (B) Histogram showing expression of the CD69 activation marker on Jurkat cells expressing exogenous TCRs from the vaccinated donor after 24 hours incubation in the presence or absence of autologous DCs and/or Fluzone 2011-2012 vaccine. Numbers of transfected cells are shown. HT-T-1 and HT-T-2, Jurkat cells transfected with TCRβ:α from peripheral TFH cells; RA14, CMV-specific TCRβ:α as a negative control. (C) CDR3 sequences and gene usages of the HT-T-1 clone. ...
Jurkat cells are an immortalized T-lymphocyte cell line, treated with serial dilutions of staurosporine, camptothecin, and etoposide and then assay using Early Tox Live/Dead Assay Kit.
DataMed is a prototype biomedical data search engine. Its goal is to discover data sets across data repositories or data aggregators. In the future it will allow searching outside these boundaries. DataMed supports the NIH-endorsed FAIR principles of Findability, Accessibility, Interoperability and Reusability of datasets with current functionality assisting in finding datasets and providing access information about them.
Western blot analysis of CRNKL1 on human Jurkat cells. The sample was probed with a CRNKL1 polyclonal antibody (Product # PA5-70817) using a primary antibody dilution of 1.0 µg/mL ...
Buy our Jurkat (human T cell lymphoblast-like cell line) whole cell lysate, tumor cell line. ab30128 has been validated in western blot. Abcam now offers…
Store-operated Ca2+ release-activated Ca2+ channels (CRAC) are very high Ca2+ selective channels composed of two protein subunits: STIM1 Ca2+sensor protein placed in the ER and ORAI1 Ca2+ channel protein in plasma membrane. ...
In this report, we describe that T cells can be modified to produce retrovirus after pharmacological induction with a rapalog dimerizer (Fig. 1) ⇓ . Furthermore, these T cells were able to deliver systemically a reporter transgene into a very significant fraction of metastatic tumor cells in the lung and liver (Fig. 2, A and B) ⇓ . When the retrovirus was modified to encode a therapeutic transgene, HSV-tk, we were able to show a significant increase in survival in mice receiving both rapalog to induce viral production and GCV as the cytotoxic prodrug in comparison with animals in which either or both drugs were withheld (Fig. 3A) ⇓ . Interestingly, for achieving a therapeutic effect, the retroviral carrier Jurkat cells had to be tumor-specific. This raises the possibility that CIR signaling results in a benefit over and above that which is produced by nonspecific cells trafficking to tumors and releasing virus. We showed in vivo that tumor specific Jurkat.CEA cells recruit parental Jurkat ...
Chemotherapy, Iron, Administration, Cell, Cell Culture, Cell Viability, Cells, Concentrations, Culture, Drug Targeting, Drugs, Flow Cytometry, Immune System, Jurkat Cell, Jurkat Cells, Leukocytes, Magnetic, Magnetic Field, Mitoxantrone, Nanoparticles
G proteins are membrane-bound molecules involved in coupling of surface receptors with signal transduction effector systems in multiple cell types including T
Z-VAD-FMK is a cell-permeable, irreversible pan-caspase inhibitor, blocks all features of apoptosis in THP.1 and Jurkat T-cells.
Jurkat nuclear extract (1 day growth) for use in WB and EMSA. For studies related to T cells, viral infection (e.g HIV), cytokines, differentiation, and cancer.
IL-2 production by JurkatEGFP and JurkatαCEA-CIR-EGFP cells stimulated either with plastic immobilized anti-CD3 mAb or target cells (E∶T = 1∶1; HeLa or
Protein C Inhibitor (PCI) is a secreted serine protease inhibitor, belonging to the family of serpins. In addition to activated protein C PCI inactivates several other proteases of the coagulation and fibrinolytic systems, suggesting a regulatory role in hemostasis. Glycosaminoglycans and certain negatively charged phospholipids, like phosphatidylserine, bind to PCI and modulate its activity. Phosphatidylerine (PS) is exposed on the surface of apoptotic cells and known as a phagocytosis marker. We hypothesized that PCI might bind to PS exposed on apoptotic cells and thereby influence their removal by phagocytosis. Using Jurkat T-lymphocytes and U937 myeloid cells, we show here that PCI binds to apoptotic cells to a similar extent at the same sites as Annexin V, but in a different manner as compared to live cells (defined spots on ∼10-30% of cells). PCI dose dependently decreased phagocytosis of apoptotic Jurkat cells by U937 macrophages. Moreover, the phagocytosis of PS exposing, activated platelets
Deficient expression of Bak in a clonal Jurkat cell line. (A) Wild-type or the variant Jurkat cell line, Bak−, were incubated in 1% NP-40 lysis buffer for 30
We have demonstrated previously the potent activation of PLD by the chemokine IL-8 in T lymphocytes (18). We have now extended our findings to include the C-C chemokine RANTES in demonstrating that in the Jurkat T cell line, the activation of this enzyme occurs at subnanomolar concentrations and is dependent on the activation of small GTP-binding protein cofactors. RANTES-induced PLD activation is consistently maximal at 1 nM, a concentration corresponding to the optimal chemotaxis-inducing dose in normal T lymphocytes. Interestingly, PLD activation in T lymphocytes and Jurkat T cells appears to be an important biologic consequence of chemokine action and more readily measurable (at nanomolar concentrations) than readouts of receptor activation such as calcium flux. It was also apparent that RANTES is the only chemokine tested to date (RANTES, MIP-1α, MIP-1β, MCP-1, MCP-3, lymphotactin) that induces as robust a response as seen in this study, although the others listed were capable of low ...
Annexin A6 (AnxA6) is a Ca2+-dependent membrane-binding protein involved in vesicular traffic. The likely participation of AnxA6 in the response of lymphocytes to Ca2+ signals has not been investigated yet. The present study focuses on intracellular relocation of AnxA6 in human Jurkat T lymphoblasts upon stimulation followed by transient increase of intracellular [Ca2+] and exocytosis of interleukin-2 (IL-2). Stimulation of the cells under different experimental conditions (by lowering pH and/or by rising extracellular [Ca2+] in the presence of ionomycin) induced time-dependent transients of intracellular [Ca2+] and concomitant changes in AnxA6 intracellular localization and in IL-2 secretion, with only minor effects on cell viability and apoptosis. In resting conditions (in the presence of EGTA or with no ionophore) AnxA6 was localized uniformly in the cytosol, whereas it translocated to vesicular structures beneath the plasma membrane within 5 min following stimulation of Jurkat T cells and ...
Necroptosis is a form of regulated necrotic cell death mediated by receptor-interacting serine/threonine-protein kinase 1 (RIPK1) and RIPK3. Necroptotic cell death contributes to the pathophysiology of several disorders involving tissue damage, including myocardial infarction, stroke and ischemia-reperfusion injury. However, no inhibitors of necroptosis are currently in clinical use. Here we performed a phenotypic screen for small-molecule inhibitors of tumor necrosis factor-alpha (TNF-α)-induced necroptosis in Fas-associated protein with death domain (FADD)-deficient Jurkat cells using a representative panel of Food and Drug Administration (FDA)-approved drugs. We identified two anti-cancer agents, ponatinib and pazopanib, as submicromolar inhibitors of necroptosis. Both compounds inhibited necroptotic cell death induced by various cell death receptor ligands in human cells, while not protecting from apoptosis. Ponatinib and pazopanib abrogated phosphorylation of mixed lineage kinase domain-like
TY - JOUR. T1 - Assessment of secondary necrosis of Jurkat cells using a new microscopic system and double staining method with annexin V and propidium iodide.. AU - Honda, O.. AU - Kuroda, Masahiro. AU - Joja, I.. AU - Asaumi, Jun-Ichi. AU - Takeda, Yoshihiro. AU - Akaki, S.. AU - Togami, I.. AU - Kanazawa, Susumu. AU - Kawasaki, S.. AU - Hiraki, Y.. PY - 2000/2. Y1 - 2000/2. N2 - Using a new system developed by us for acquiring microscopic images automatically, we compared the morphological changes that apoptotic cells undergo with changes in the staining pattern of annexin V-enhanced green fluorescent protein (AV-EGFP) and propidium iodide (PI) in individual cells. Jurkat cells were treated with 5 mM CaCl2 alone, anti-Fas antibody and heating at 42 degrees C for 30 min or 46 degrees C for 60 min, and then were incubated in medium with 5 mM CaCl2. Time-lapse DNA fragmentation analysis and morphological observation revealed that the anti-Fas antibody and heating at 42 degrees C for 30 min ...
The ς binding site present in the Jurkat human T lymphocyte cell line was investigated. Jurkat cell membranes were found to have a single saturable binding site for [3H]haloperidol, a ς ligand (dissociation constant, 3.9 ± 0.3 nM). The binding of [3H]haloperidol was inhibited by several ς ligands. Northern analysis and reverse transcription-polymerase chain reaction provided evidence for the expression of the recently cloned type 1 ς-receptor (ς-R1) in Jurkat cells. The ς-R1 cDNA cloned from these cells was functional in heterologous expression systems. When expressed in mammalian cells, the cDNA-induced binding was saturable with dissociation constants of 1.9 ± 0.3 nM for [3H]haloperidol and 12 ± 2 nM for (+)-pentazocine. The binding of [3H]progesterone, a putative endogenous ligand to ς-R1, to the Jurkat cell ς-receptor could be directly demonstrated by using heterologously expressed ς-R1 cDNA. The binding of [3H]progesterone was saturable, with a dissociation constant of 88 ± 7 ...
TY - JOUR. T1 - Signaling through T lymphocyte surface proteins, TCR/CD3 and CD28, activates the HIV-1 long terminal repeat. AU - Tong-Starksen, S. E.. AU - Luciw, Paul A. AU - Peterlin, B. M.. PY - 1989. Y1 - 1989. N2 - The state of T cell activation and proliferation controls HIV-1 replication and gene expression. Previously, we demonstrated that the administration of PHA and PMA to the human T cell line Jurkat activates the HIV-1 enhancer, which is composed of two nuclear factor κB (NFκB) binding sites. Here, we show that PMA alone is sufficient for this effect. In addition, activation of T cells through the surface proteins TCR/CD3 and CD28 increased gene expression directed by the HIV-1 long terminal repeat (LTR) to the same extent as PMA. Analysis of 5 deletions in the LTR revealed that the NFκB binding sites and sequences in the upstream U3 region are required for this response. Whereas cyclosporin A did not inhibit the effect of PMA, it reduced the effects of agonists to TCR/CD3 and ...
TY - JOUR. T1 - Lysophosphatidylcholine is a regulator of tyrosine kinase activity and intracellular Ca2+ level in Jurkat T cell line. AU - Légrádi, Ádám. AU - Chitu, Violeta. AU - Szukacsov, Valéria. AU - Fajka-Boja, Roberta. AU - Szücs, Kinga Székely. AU - Monostori, Éva. PY - 2004/1/30. Y1 - 2004/1/30. N2 - Lysophospholipids, particularly lysophosphatidylcholine (lyso-PC), have been implicated in modulating T cell functions at the sites of inflammation and atherosclerosis. Although the chemotactic and immunomodulatory effects are well documented, the exact signaling pathway of lyso-PC action is poorly defined. In this work, we studied the earliest biochemical events in T cells triggered by lyso-PC. A marked and immediate tyrosine phosphorylation was induced in the leukemic T cell line, Jurkat. Phosphorylation of cellular substrates included src family kinase, p56lck and syk family kinase, ZAP70. The lyso-PC induced tyrosine phosphorylation was largely dependent on the presence of ...
Jurkat E6.1 Cell Line human from human blood(leukemic T-cell lymphoblast), 88042803; find null-null MSDS, related peer-reviewed papers, technical documents, similar products & more at Sigma-Aldrich.
Calcium release-activated calcium channels (CRAC) control influx of calcium in human T lymphocytes. Hour-long calcium elevations are necessary for efficient gene expression during T cell activation and proliferation. We report here that, the time course for store-operated Ca2+ entry is short-lived (3-4 min) and therefore, cannot account for the prolonged Ca2+ elevations necessary for NFAT translocation into nucleus. Previous findings strongly suggest that T cell activation is accompanied by cytosolic alkalinization. Here, we show that pH changes in Jurkat T cells following activation with mitogenic lectin, phytohemagglutinin (PHA), depends on the length of time of exposure and the concentration (potency) of the mitogen. For full understanding of ion fluxes involved in this process, it is important to distinguish CRAC channel subtype functions in these cells during activation as well as elucidate the pH mediated changes in Ca2+. In some experiments we show low pH with high concentrations of PHA. We also
Ca2+ release from intracellular stores is one of the major events transducing extracellular signals into living cells. Recently, a metabolite of nicotinamide adenine dinucleotide+ (NAD+), termed cyclic adenosine diphosphate-ribose (cADPr), has been described to release Ca2+ from caffeine-sensitive internal stores of cells. Jurkat T cells possess intracellular Ca2+ stores sensitive to caffeine, so a potential involvement of cADPr in Ca2+ signaling was investigated. cADPr released Ca2+ in a dose-dependent manner from intracellular stores of permeabilized Jurkat T cells. Half maximal release was obtained at 2.25 microM cADPr. Prior addition of D-myo-inositol 1,4,5-trisphosphate (Ins(1,4,5)P3) or thapsigargin did not influence cADPr-induced Ca2+ release, indicating the presence of different Ca2+ pools sensitive to Ins(1,4,5)P3 and cADPr. The specificity of the response was confirmed using the inhibitors ruthenium red, 8-NH2-cADPr, and 8-Br-cADPr. All three compounds blocked cADPr-induced, but not Ins(1,4
© 2015 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim. T lymphocytes need to detect rare cognate foreign peptides among numerous foreign and self-peptides. This discrimination seems to be based on the kinetics of TCRs binding to their peptide-MHC (pMHC) ligands, but there is little direct information on the minimum time required for processing elementary signaling events and deciding to initiate activation. Here, we used interference reflection microscopy to study the early interaction between transfected human Jurkat T cells expressing the 1G4 TCR and surfaces coated with five different pMHC ligands of 1G4. The pMHC concentration required for inducing 50% maximal IFN-γ production by T cells, and 1G4-pMHC dissociation rates measured in soluble phase or on surface-bound molecules, displayed six- to sevenfold variation among pMHCs. When T cells were dropped onto pMHC-coated surfaces, rapid spreading occurred after a 2-min lag. The initial spreading rate measured during the first 45 s, and the contact area
Our previous experiments have shown that MMTV Rem functions in nuclear export of unspliced viral RNA in rodent cells [5]. In this manuscript, we have shown that Rem functions in human cell lines. Our results also indicate that Rev and Rex can increase reporter gene expression by interaction with the MMTV RmRE in human Jurkat T cells (Figures 2 and 4). Rex also could function on the RmRE in 293T HEK cells. Prior data indicate that some retroviral export proteins function on heterologous retroviral RNAs. For example, Rex can bind and function on both the RRE and the RxRE [43, 44]. However, the interaction is not reciprocal since Rev cannot act on the RxRE [43]. In this respect, the RmRE is quite permissive since it is required for enhancement of luciferase activity by Rem, Rev and Rex in human T cells. No effect of Rem was observed on the HIV and HTLV response elements (Figure 8). Surprisingly, the Rec export protein from the human retrovirus most closely related to MMTV, HERV-K/HML, had no effect ...
HIV-1 is transcriptionally active in activated T helper (Th)-cells and inactive in naive or resting memory Th-cells. Ets-2 is a preinduction transcriptional repressor of the IL-2 gene in naive Th-cells and a candidate transcriptional repressor of HIV-1 in the same cells, because the −279 to −250 upstream region of HIV-1-LTR [repressor-activator target sequence (RATS)], that participates in HIV-1-LTR transcriptional silencing, encompasses the AAGGAG Ets-2 binding site. In this proof of concept study, we investigated whether Ets-2 represses the expression of HIV-1. To assess whether Ets-2 can repress HIV-1 transcriptional activation acting through RATS, we transfected Jurkat cells with an Ets-2 overexpression plasmid (pCDNA3-ets-2) or Ets-2 silencing plasmids (ets-2-shRNA) and, as target genes, plasmids carrying the whole HIV-1-LTR sequence (HIV-1-LTR-CAT) or two copies of the RATS sequence (2× RATS-CAT) or a point mutation in the Ets-2 binding site (2× mutantRATS-CAT) or CMV-CAT (control). Ets-2
TY - JOUR. T1 - Determination of cytokine regulated glycan expression by using molecularly imprinted polymers targeting sialic acid. AU - Shinde, Sudhirkumar. AU - El-Schich, Zahra PY - 2019/7/11. Y1 - 2019/7/11. N2 - Cancer cells often have an increased amount of glycans, such as sialic acid (SA), on the cell surface, which normallyplay an important role in cell growth, proliferation and differentiation. In this study, SA expression is determinedby fluorescent nanoprobes, molecularly imprinted polymers, SA-MIPs. The nanoprobes are synthesized with animprinting approach to produce tailor-made fluorescent core-shell particles with high affinity for cell surface SA.Inflammation and cytokine production are well known tumor promoters, modulating the cellular microenvironment,including an aberrant cell surface glycan pattern. The recombinant cytokines IL-4, IL-6, IL-8 and a cocktail ofcytokines collected from stimulated T leukemia Jurkat cells were used to induce in vitro inflammation in two ...
The mechanism through which CD28 costimulation potentiates TCR-driven gene expression is still not clearly defined. Vav-1, an exchange factor for Rho GTPases thought to regulate, mainly through Rac-1, various signaling components leading to cytokine gene expression, is tyrosine phosphorylated upon CD28 engagement. Here, we provide evidence for a key role of Vav-1 in CD28-mediated signaling. Overexpression of Vav-1 in Jurkat cells in combination with CD28 ligation strongly reduced the concentration of staphylococcus enterotoxin E/MHC required for TCR-induced NF-AT activation. Surprisingly, upon Vav-1 overexpression CD28 ligation sufficed to activate NF-AT in the absence of TCR engagement. This effect was not mediated by overexpression of ZAP-70 nor of SLP-76 but necessitated the intracellular tail of CD28, the intactness of the TCR-proximal signaling cascade, the Src-homology domain 2 (SH2) domain of Vav-1, and SLP-76 phosphorylation, an event which was favored by Vav-1 itself. Cells overexpressing Vav-1
Chang PY, Draheim K, Kelliher MA, Miyamoto S. NFKB1 is a direct target of the TAL1 oncoprotein in human T leukemia cells. Cancer Res. 2006 Jun 15; 66(12):6008-13 ...
hi! is anyone out there tried electroporating Jurkat cells? If so, can you post the electroporation conditions please. cheers venkat -- Venkat Pisupati Lab -44-01223-334131 Wellcome/CRC Institute Fax -44-01223-334134 Dept. of Genetics E.mail. vnp at mole.bio.cam.ac.uk University of Cambridge UK CB2 1QR ...
For three years, I worked at NIH, in the NICHD. I took part in the research being conducted in the lab of Dr. Brant Weinstein, working with vascular morphogenesis in embryonic zebrafish. From Jan 2008-Jan 2009, I worked with Dr. Andreas Herrlich in the Lodish lab at the Whitehead. We performed a large-scale high-throughput screen to test the effects a set of shRNAs designed to knock-down most human kinases and phophatases has on ectodomain cleavage. Specifically, we used osmotic stress as a cleavage stimulus to the EGF ligand TGF-alpha. We worked in human Jurkat cells, and mouse Baf3 cell lines. Previous 20.109 labwork [1] Currently, I work in the lab of Dr. Ed Boyden. I am involved in the Epilepsy research group, an attempt to cause and then silence seizure activity in mice using optically controlled neurons. Over the summer, I am also working with the molecular biology group, to clone multiple new mutants of rhodopsins into usable backbones, which will then be used to generate virus capable of ...
Fingerprint Dive into the research topics of Biotin requirements are lower in human Jurkat lymphoid cells but homeostatic mechanisms are similar to those of HepG2 liver cells. Together they form a unique fingerprint. ...
Anti-Cancer Properties. A team of Iranian researchers at the Vaccine and Serum Research Institute in Mashhad conducted in vitro tests to evaluate the ability of dried jujube extract to inhibit growth or induce cell death in a variety of human tumor cell lines. Scientists used the MTT colorimetric assay to measure the degree to which water extract of dried jujube reduced the proliferation of tumor cells. Although the jujube extract inhibited tumor cell proliferation in all lines, it displayed the greatest effect against Jurkat leukemia cells. In a report in the February 2008 issue of Cytotechnology, researchers said their findings confirm dried jujubes cytotoxic properties against a variety of cancer cell lines and urged further study to identify the mechanisms through which jujube works.. Anti-Inflammatory. Diabetics can benefit from jujube fruit when it is used as a medicinal rub for cuts and sores. Many diabetics suffer from poor circulation and neuropathy, which causes nerve damage, ...
One cryopreserved vial of immortalised human T lymphocyte cells stably expressing a nuclear restricted red fluorescent protein (1.5 x 106 cell/vial) Jurkat Incucyte® NucLight Red Cells are supplied as 1 mL cryopreserved vials (1.5 x 106 cells/mL in 90% FBS and 10% DMSO) containing a stable population of human T lymphoc
Plasmid NFAT/AP-1 3x luciferase from Dr. Anjana Raos lab contains the insert NFAT/AP-1 3x and is published in EMBO J. 2000 Sep 1. 19(17):4783-95. This plasmid is available through Addgene.
Thank you for your interest in spreading the word about The Journal of Immunology.. NOTE: We only request your email address so that the person you are recommending the page to knows that you wanted them to see it, and that it is not junk mail. We do not capture any email address.. ...
DOK3 Antibody 15749-1-AP has been identified with ELISA, WB, IHC. 15749-1-AP detected 53kd band in Jurkat cells with 1:500-1:1000 dilution...
NOD2 Antibody 20980-1-AP has been identified with ELISA, IF, IHC, WB. 20980-1-AP detected 100-110 kDa band in Jurkat cells with 1:500-1:1000 dilution...
WMF: please dont send me spam-mails again. Dont ever ask me again to translate articles into a language I do not speak. Especially if I have no clue what they are about (en:Cell migration, en:Subject-matter jurisdiction, en:Jurkat cells) and I am totally not interest in (en:Connie Sawyer, en:House of Kamehameha). This is not the kind of mail I left my e-mail-address for. I am interested in pigeons and poultry. If I ever felt like translating something, this would be the very good czech-pigeon-articles into de-wikipedia. ...
... lung adenocarcinoma A549 cells; human colorectal cancer cells; corneal epithelial cells; and Jurkat T-cell leukemia cells. The ... human cancer colon cells, human hepatocellular HepG2 and SMMC7721 cancer cells; mouse 3T3 cells (a fibroblast cell line); rat ... These species trigger cells to activate their death programs, i.e. apoptosis, and/or are openly toxic to the cells. 15(S)-HpETE ... The initially formed 15(S)-HpETE may be further metabolized by its parent cell or pass it to nearby cell by a process termed ...
... regulation by Ets in Jurkat T cells". J Cell Biochem. 72 (4): 492-506. doi:10.1002/(SICI)1097-4644(19990315)72:4. 3.0.CO;2-H. ... Cell. Endocrinol. 133 (2): 177-82. doi:10.1016/S0303-7207(97)00148-2. PMID 9406864. S2CID 43782586. Asa SL, Ramyar L, Murphy PR ... 2001). "The endogenous fibroblast growth factor-2 antisense gene product regulates pituitary cell growth and hormone production ... application for identifying ubiquitinated proteins in human cells". J. Proteome Res. 6 (1): 298-305. CiteSeerX 10.1.1.401.4220 ...
Némorin JG, Laporte P, Bérubé G, Duplay P (2001). "p62dok negatively regulates CD2 signaling in Jurkat cells". J. Immunol. 166 ... Cell. Biol. 23 (8): 2658-68. doi:10.1128/MCB.23.8.2658-2668.2003. PMC 152553. PMID 12665569. Master Z, Tran J, Bishnoi A, et al ... Master Z, Jones N, Tran J, Jones J, Kerbel RS, Dumont DJ (2001). "Dok-R plays a pivotal role in angiopoietin-1-dependent cell ... This encoded protein binds p120 (RasGAP) from CML cells. DOK2 has been shown to interact with INPP5D and TEK tyrosine kinase. ...
"ICBP90 expression is downregulated in apoptosis-induced Jurkat cells". Annals of the New York Academy of Sciences. 1010: 300-3 ... in tumor cell growth". Molecular Biology of the Cell. 16 (12): 5621-9. doi:10.1091/mbc.E05-03-0194. PMC 1289407. PMID 16195352 ... Its expression peaks at late G1 phase and continues during G2 and M phases of the cell cycle. It plays a major role in the G1/S ... Cancer Cell. 25 (2): 196-209. doi:10.1016/j.ccr.2014.01.003. PMC 3951208. PMID 24486181. Ku DH, Chang CD, Koniecki J, ...
kamtschaticus has shown potent cytotoxicity against Jurkat T cells. Aruncus dioicus var. aethusifolius (H.Lév.) H.Hara - Korean ...
"Caspase-mediated cleavage of TRAF3 in FasL-stimulated Jurkat-T cells". Journal of Leukocyte Biology. 69 (3): 490-6. PMID ... "The cytoplasmic domain of the lymphotoxin-beta receptor mediates cell death in HeLa cells". The Journal of Biological Chemistry ... Saha SK, Cheng G (April 2006). "TRAF3: a new regulator of type I interferons". Cell Cycle. 5 (8): 804-7. doi:10.4161/cc.5.8. ... Cell. 84 (2): 299-308. doi:10.1016/S0092-8674(00)80984-8. PMID 8565075. Hsu H, Huang J, Shu HB, Baichwal V, Goeddel DV (April ...
2007). "Cell-cycle-dependent regulation of Ca2+-activated K+ channel in Jurkat T-lymphocyte". J. Pharmacol. Sci. 104 (1): 94-8 ... 2001). "Ca2+-activated K+ channels in human leukemic Jurkat T cells. Molecular cloning, biochemical and functional ... "SK2 encodes the apamin-sensitive Ca2+-activated K+ channels in the human leukemic T cell line, Jurkat". FEBS Lett. 469 (2-3): ... 2006). "Ca2+-activated K+ channels in human melanoma cells are up-regulated by hypoxia involving hypoxia-inducible factor-1α ...
... induces apoptosis and caspase-dependent degradation of haematopoietic lineage cell-specific protein 1 (HS1) in Jurkat cells". ... Abdul-Ghani M, Megeney LA (June 2008). "Rehabilitation of a contract killer: caspase-3 directs stem cell differentiation". Cell ... Hsp60 and Hsp10 in the mitochondrial fraction of jurkat cells". The EMBO Journal. 18 (8): 2040-8. doi:10.1093/emboj/18.8.2040. ... "Caspase-mediated cleavage of TRAF3 in FasL-stimulated Jurkat-T cells". Journal of Leukocyte Biology. 69 (3): 490-6. PMID ...
"Elongation factor-1alpha as a homologous complement activator of Jurkat cells". International Journal of Molecular Medicine. 6 ...
"Deficiency of ADAP/Fyb/SLAP-130 destabilizes SKAP55 in Jurkat T cells". The Journal of Biological Chemistry. 280 (25): 23576-83 ... Wang H, Liu H, Lu Y, Lovatt M, Wei, B, Rudd CE Functional defects of SKAP-55 deficient T-cells identify a regulatory role for ... Jo EK, Wang H, Rudd CE (June 2005). "An essential role for SKAP-55 in LFA-1 clustering on T cells that cannot be substituted by ... Cell. Biol. 2007 27, 6863-75. * Kosco KA, Cerignoli F, Williams S, Abraham RT, Mustelin T (January 2008). "SKAP55 modulates T ...
"Deficiency of ADAP/Fyb/SLAP-130 destabilizes SKAP55 in Jurkat T cells". J. Biol. Chem. 280 (25): 23576-83. doi:10.1074/jbc. ... proteins and the Arp2/3 complex link T cell receptor (TCR) signaling to the actin cytoskeleton". J. Cell Biol. 149 (1): 181-94 ... da Silva AJ, Li Z, de Vera C, Canto E, Findell P, Rudd CE (1997). "Cloning of a novel T-cell protein FYB that binds FYN and SH2 ... da Silva AJ, Janssen O, Rudd CE (1993). "T cell receptor zeta/CD3-p59fyn(T)-associated p120/130 binds to the SH2 domain of ...
Possible human expression of TMEM143 protein occurs in Jurkat cells (T lymphocyte). Organelle association puts TMEM143 in the ... This indicates the possibility of TMEM143 participation in lipid metabolic pathways and lipid cell differentiation "TMEM143 ... indicating the location of the protein inside the cell. Orthologs have been identified in more than 85 vertebrate species. No ...
Luo H, Wan X, Wu Y, Wu J (2001). "Cross-linking of EphB6 resulting in signal transduction and apoptosis in Jurkat cells". J. ... 2000). "T-cell-specific expression of kinase-defective Eph-family receptor protein, EphB6 in normal as well as transformed ... Eph Nomenclature Committee". Cell. 90 (3): 403-4. doi:10.1016/S0092-8674(00)80500-0. PMID 9267020. S2CID 26773768. Hock B, ... hematopoietic cells". Growth Factors. 18 (1): 63-78. doi:10.3109/08977190009003234. PMID 10831073. S2CID 9397812. Tang XX, Zhao ...
2005). "Extracellular pH modifies mitochondrial control of capacitative calcium entry in Jurkat cells". J. Biol. Chem. 280 (5 ... Discovery of the pH sensitivity of calcium entry into mammalian cells (the calcium concentration within the cell serves as a ... He specialises in various issues related to biochemistry, including bioenergetics, the role of mitochondria in cell physiology ... features which produce abnormal diffusion of metabolites within the cell as well as influence transport across the plasma ...
"Caspase-dependent activation of cyclin-dependent kinases during Fas-induced apoptosis in Jurkat cells". Proceedings of the ... "Rapid microtubule-independent dynamics of Cdc20 at kinetochores and centrosomes in mammalian cells". The Journal of Cell ... "Rapid microtubule-independent dynamics of Cdc20 at kinetochores and centrosomes in mammalian cells". The Journal of Cell ... Cell division cycle protein 27 homolog is a protein that in humans is encoded by the CDC27 gene. The protein encoded by this ...
In 2013, Runne et al., showed that PLEKHG2 is elevated in several leukemia cell lines, including Jurkat T cells. In addition, ... B cell and T cell leukemia at high frequency. In 2002, Himmel et al., used this model of acute myelogenous leukemia and showed ... by epidermal growth factor receptor signaling regulates cell morphology of Neuro-2a cells". The Journal of Biological Chemistry ... In this cell the Gβγ subunit of the trimeric G protein were interacted with PLEKHG2 directly. Ueda and colleagues also showed ...
Lindholm CK, Henriksson ML, Hallberg B, Welsh M (July 2002). "Shb links SLP-76 and Vav with the CD3 complex in Jurkat T cells ... The protein is important in hematopoiesis, playing a role in T-cell and B-cell development and activation. This particular GEF ... linker for activation of T cells) required for recruitment and activation of signalling proteins in T cells". Biochem. J. 356 ( ... Cell. Biol. 16 (1): 37-44. doi:10.1128/mcb.16.1.37. PMC 230976. PMID 8524317. Paz PE, Wang S, Clarke H, Lu X, Stokoe D, Abo A ( ...
Nov 2001). "Hypoxia induces apoptosis via two independent pathways in Jurkat cells: differential regulation by glucose". ... Brain cells are very sensitive to reduced oxygen levels. Once deprived of oxygen they will begin to die off within five minutes ... During oxygen deprivation, cells die due to an increasing acidity in the brain tissue (acidosis). Additionally, during the ... Cell Physiology. 281 (5): C1596-603. doi:10.1152/ajpcell.2001.281.5.c1596. PMID 11600423. Mattiesen W. R.; et al. (May 2009). " ...
"Increased expression of Lewis X and Y antigens on the cell surface and FUT 4 mRNA during granzyme B-induced Jurkat cell ... "Expression of cell surface Lewis X and Y antigens and FUT4 mRNA is increased in Jurkat cells undergoing apoptosis". Biochim. ... "Overexpression of fucosyltransferase IV in A431 cell line increases cell proliferation". Int. J. Biochem. Cell Biol. 39 (9): ... "Induction of FucT-VII by the Ras/MAP kinase cascade in Jurkat T cells". Blood. 102 (5): 1771-8. doi:10.1182/blood-2002-11-3551 ...
Lindholm CK, Henriksson ML, Hallberg B, Welsh M (July 2002). "Shb links SLP-76 and Vav with the CD3 complex in Jurkat T cells ... SLP-76 might serve as an integration point for signals by activating NK cell receptors. In NK cells, SLP-76 can be ... ligation in the leukemic T cell line Jurkat. The SLP-76 locus has been localized to human chromosome 5q33 and the gene ... SH3 domain-binding site in SLP-76 required for T-cell receptor-mediated activation of PLC-gamma1 and NFAT". Mol. Cell. Biol. 21 ...
Lindholm CK, Henriksson ML, Hallberg B, Welsh M (Jul 2002). "Shb links SLP-76 and Vav with the CD3 complex in Jurkat T cells". ... "Expression of the Grb2-related protein of the lymphoid system in B cell subsets enhances B cell antigen receptor signaling ... GRAP2 has been shown to interact with: CCNDBP1, CD28, Linker of activated T cells, Lymphocyte cytosolic protein 2 MAP4K1, and ... Liu SK, Fang N, Koretzky GA, McGlade CJ (Jan 1999). "The hematopoietic-specific adaptor protein gads functions in T-cell ...
"Caspase-dependent activation of cyclin-dependent kinases during Fas-induced apoptosis in Jurkat cells". Proc. Natl. Acad. Sci. ... Zhao RY, Elder RT (2005). "Viral infections and cell cycle G2/M regulation". Cell Res. 15 (3): 143-9. doi:10.1038/sj.cr.7290279 ... Baldin V, Ducommun B (1995). "Subcellular localisation of human wee1 kinase is regulated during the cell cycle". J. Cell Sci. ... and G2-M cell population". Cell Growth Differ. UNITED STATES. 11 (4): 211-9. ISSN 1044-9523. PMID 10775038. Shen, M; Stukenberg ...
... cell receptor-dependent activation of the interleukin-2 gene nuclear factor for activation of T cells element in Jurkat T cells ... cell receptor-dependent activation of the interleukin-2 gene nuclear factor for activation of T cells element in Jurkat T cells ... Lindholm CK, Henriksson ML, Hallberg B, Welsh M (Jul 2002). "Shb links SLP-76 and Vav with the CD3 complex in Jurkat T cells". ... Lindholm CK, Henriksson ML, Hallberg B, Welsh M (Jul 2002). "Shb links SLP-76 and Vav with the CD3 complex in Jurkat T cells". ...
"The lymphocyte-specific tyrosine protein kinase p56lck is endocytosed in Jurkat cells stimulated via CD2". J. Immunol. 148 (12 ... is a cell adhesion molecule found on the surface of T cells and natural killer (NK) cells. It has also been called T-cell ... CD2 is a specific marker for T cells and NK cells, and can therefore be used in immunohistochemistry to identify the presence ... The great majority of T cell lymphomas and leukaemias also express CD2, making it possible to use the presence of the antigen ...
This kinase was shown to be activated rapidly during Fas-mediated apoptosis in Jurkat cells. In response to Fas receptor ... Mol Cell Biol. 24 (24): 10718-32. doi:10.1128/MCB.24.24.10718-10732.2004. PMC 533970. PMID 15572676. "Entrez Gene: FASTK Fas- ...
This choice was made as TGN1112 showed antibody-dependent cellular cytotoxicity on CD28+ Jurkat cells. Thus the function of ... Thus, attempts to induce FOXP3+ T cells might also induce effector cells capable of causing tissue damage. Other cells ... However, cell opsonisation by antibody leads normally to phagocytosis of the labeled cells, as seen in the case of HIV. The ... However, eventually most of these cells downregulate their regulatory capabilities and become effector cells. ...
"Type II phosphatidylinositol 4-kinase beta associates with TCR-CD3 zeta chain in Jurkat cells". Mol. Immunol. 43 (5): 454-63. ... Cell. 23 (5): 685-95. doi:10.1016/j.molcel.2006.07.014. PMID 16949365. Srivastava R, Sinha RK, Subrahmanyam G (2006). " ...
Akao Y, Nakagawa Y, Iio A, Naoe T (2009). "Role of microRNA-143 in Fas-mediated apoptosis in human T-cell leukemia Jurkat cells ... in combination with miR-506 has shown to be effective in blocking cell cycle progression of lung cancer cell lines. Moreover, ... 145 alters smooth muscle cell maintenance and vascular homeostasis in mice: correlates with human disease". Cell Death Differ. ... mir-143 was found to be the most enriched miRNA in mouse embryonic stem cells that were differentiating into cardiac progenitor ...
Effect of knocking down eEF1A1 gene on proliferation and apoptosis in Jurkat cells and its mechanisms]". Zhongguo Shi Yan Xue ... Though it has not been observed to localize to the cell membrane, it can be found in the outer cell surface. Its role in ... knocking down the eEF1A1 gene produces inhibited proliferation and induced apoptosis of Jurkat cells. These effects may be ... eEF1A1 is expressed in most cells while eEF1A2 is only expressed in adult neuronal and muscle cells, and only eEF1A1 induces ...
Similarly, FICZ has been identified and quantified in Jurkat cells grown in L-Trp enriched medium. FICZ was first identified in ... Various effects of the AHR and FICZ in cancer cells and cancer stem cells (CSCs) have also been described. The AHR is involved ... there seems to be no intrinsic difference in the effects of FICZ and TCDD on T cell differentiation and T cell-mediated ... such as intra-thymic progenitor cells, as well as hematopoietic, pulmonary, and neuro-epithelial stem cells. The AHR seems to ...
1992). "The lymphocyte-specific tyrosine protein kinase p56lck is endocytosed in Jurkat cells stimulated via CD2.". J. Immunol. ... 1997). "CD2 induced apoptosis of peripheral T cells.". Transplant. Proc. 29 (5): 2377-8. PMID 9270771. doi:10.1016/S0041-1345( ... Peterson A, Seed B (1987). "Monoclonal antibody and ligand binding sites of the T cell erythrocyte receptor (CD2).". Nature 329 ... Yang J, Ye Y, Carroll A, Yang W, Lee H (2001). "Structural biology of the cell adhesion protein CD2: alternatively folded ...
Nov 2001). "Hypoxia induces apoptosis via two independent pathways in Jurkat cells: differential regulation by glucose". ... Cell Physiology. 281 (5): C1596-603. doi:10.1152/ajpcell.2001.281.5.c1596. PMID 11600423.. ... Brain cells are very sensitive to reduced oxygen levels. Once deprived of oxygen they will begin to die off within five minutes ... During oxygen deprivation, cells die due to an increasing acidity in the brain tissue (acidosis). Additionally, during the ...
1995). «Spatial association of HIV-1 tat protein and the nucleolar transport protein B23 in stably transfected Jurkat T-cells ... cell volume homeostasis. • nucleocytoplasmic transport. • protein localization. • positive regulation of cell proliferation. • ... J Cell Biol. 183 (4): 589-95. PMC 2582899. . PMID 19015314. doi:10.1083/jcb.200807185. !CS1 manut: Uso explícito de et al. ( ... Cell. Biol. 11 (5): 2567-75. PMC 360026. . PMID 2017166. !CS1 manut: Uso explícito de et al. (link) !CS1 manut: Nomes múltiplos ...
2002). "CCR6 colocalizes with CD18 and enhances adhesion to activated endothelial cells in CCR6-transduced Jurkat T cells". J. ... 1997). "A somatic cell hybrid panel for distal 17q: GDIA1 maps to 17q25.3". Cytogenet. Cell Genet. 76 (3-4): 172-5. doi:10.1159 ... positive regulation of dendritic cell chemotaxis. • signal transduction. • calcium-mediated signaling. • chemotaxis. • cell ... positive regulation of epithelial cell migration. • dendritic cell chemotaxis. • cellular defense response. • humoral immune ...
p66SHC inhibits ERK1/2 activity and antagonize mitogenic and survival abilities of T-lymphoma Jurkat cell lines.[8] A rise in ... cell-cell adhesion. • interleukin-15-mediated signaling pathway. • negative regulation of angiogenesis. • cytokine-mediated ... Wrighton KH (Aug 2013). "Cell signalling: EGF signalling--it's all in SHC1's timing". Nature Reviews Molecular Cell Biology. 14 ... When SgK269 is overexpressed in mammary epithelial cells it promotes the cell growth and might contribute to the progression of ...
IMMUNOCAL on growth of mammary carcinoma cells and Jurkat T cells in comparison to normal peripheral blood mononuclear cells. ... The spleen cells immune response to sheep red blood cells of C3H/HeJ mice fed a 20 g whey protein/100 g diet is substantially ... The plaque-forming cell response to sheep red blood cells was found to be enhanced in mice fed a formula diet containing 20 g ... 4. Differential Effect Of Dietary Protein Type On The B-Cell And T-Cell Immune Responses In Mice Bounous G., Kongshavn P.A. J ...
"Altered expression of glycoproteins on the cell surface of Jurkat cells during etoposide-induced apoptosis: shedding and ... 2010). "Lysyl tRNA synthetase is required for the translocation of calreticulin to the cell surface in immunogenic death". Cell ... 2010). "A mechanism of release of calreticulin from cells during apoptosis". J. Mol. Biol. 401 (5): 799-812. PMID 20624402. doi ... Cell Biol. 37 (2): 260-6. PMID 15474971. doi:10.1016/j.biocel.2004.02.030.. ...
Ruegg CL, Strand M (1990). "Inhibition of protein kinase C and anti-CD3-induced Ca2+ influx in Jurkat T cells by a synthetic ... regulation of cell growth. • positive regulation of T cell proliferation. • positive regulation of telomerase activity. • cell ... Cell. Biol. 16 (10): 5782-91. PMC 231579 . PMID 8816492.. *. Holmes AM (1996). "In vitro phosphorylation of human ... T cell receptor signaling pathway. • regulation of platelet aggregation. • execution phase of apoptosis. • regulation of ...
Hedin KE, Bell MP, Kalli KR, Huntoon CJ, Sharp BM, McKean DJ (Dec 1997). "Delta-opioid receptors expressed by Jurkat T cells ... in cells that coexpress both receptors compared to those in cells that express them individually. In addition, work by Fan and ... The human delta -opioid receptor displays constitutive oligomerization at the cell surface, which is not regulated by receptor ... hybrid cells". The Journal of Biological Chemistry. 266 (6): 3365-8. PMID 1671672.. ...
Lühikokkuvõte., Cell Calcium. 2013 apr; 53 (4): 241-255. doi: 10.1016/j.ceca.2013.01.001. Epub 26. jaanuar 2013 ... Frank Lehmann-Horn, Karin Jurkat-Rott, Voltage-Gated Ion Channels and Hereditary Disease, Physiological Reviews, 10. jaanuar ... Cell, 158(5), 977-979.http://www.sciencedirect.com/science/article/pii/S0092867414010344 ... Roles of IP3R and RyR Ca2+ channels in endoplasmic reticulum stress and β-cell death. Diabetes, 58(2), 422-432.http://diabetes. ...
Jurkat cells, (T lymphocye cell line), Hut78 cells (T cell lymphoma cell line), HEK 293 cells (primary embryonic kidney cell ... Further studies in animal models suggest that the 12S-HETE made by pancreatic beta cells (or possibly alpha cells or other cell ... GPR31 mRNA is expressed at low levels in several human cell lines including K562 cells (human myelogenous leukemia cell line), ... line), MCF7 cells (mammary adenocarcinoma cell line), and EJ cells (bladder carcinoma cell line). This mRNA appears to be more ...
... type-1 Tat protein selectively stimulates a phosphatidylinositol-specific phospholipase C nuclear pathway in the Jurkat T cell ... Cell. Signal. 16 (8): 921-8. PMID 15157671. doi:10.1016/j.cellsig.2004.01.009.. ... 5-trisphosphate-regulated stores of intracellular calcium in calcium dysregulation and neuron cell death caused by HIV-1 ... "Activation of CD4 T cells by Raf-independent effectors of Ras". Proc. Natl. Acad. Sci. U.S.A. 100 (10): 6003-8. PMC 156316 ...
... down-regulation of gene expression by PMA and calcium ionophore in Jurkat T lymphoma cells". Biochemical and Biophysical ... "A novel cell-cell junction system: the cortex adhaerens mosaic of lens fiber cells". Journal of Cell Science 116 (Pt 24): 4985- ... "Ksp-cadherin is a functional cell-cell adhesion molecule related to LI-cadherin". Experimental Cell Research 294 (2): 345-55. ... Dobrosotskaya IY, James GL (Apr 2000). "MAGI-1 interacts with beta-catenin and is associated with cell-cell adhesion structures ...
... prove that theory was based on the introduction of mutated form of this protein inside both TF-1 human cells and Jurkat cells, ... Apoptosis is a cell self-destruct process that removes toxic and/or useless cells during mammalian development and other life ... The cell diversity is originated by cell differentiation, which has been attributed to the activation of specific transcription ... Despite this gene being present in every cell, this protein is only expressed in different tissues and cell variety such as ...
Additionally, NADA has been observed to suppress inflammatory activation of human Jurkat T cells and to inhibit the release of ... Finally, NADA can prevent the degranulation and release of TNF from RBL- 2H3 mast cells treated with an IgE-antigen complex. ... "Inhibitory effect of N-Acyl dopamines on IgE-mediated allergic response in RBL-2H3 cells". Lipids. 48 (4): 383-393. doi:10.1007 ... NADA also promotes the inflammatory resolution of human endothelial cells activated by both endogenous (i.e. TNF) and exogenous ...
... in reticulocyte lysates and affects the expression of nuclear proteins upon stable transfection into Jurkat T-lymphoma cells". ... The protein can inhibit cell-free translation of mRNAs, suggesting that it plays a regulatory role in the translation apparatus ... Neumann F, Hemmerich P, von Mikecz A, Peter HH, Krawinkel U (January 1995). "Human ribosomal protein L7 inhibits cell-free ... Wool IG, Chan YL, Glück A (1996). "Structure and evolution of mammalian ribosomal proteins". Biochemistry and Cell Biology. 73 ...
... flow and actomyosin II arc contraction drive receptor cluster dynamics at the immunological synapse in Jurkat T cells". Mol ... F-Tractin is one of a number of F-actin probes useful in live cell imaging. Schell MJ, Erneux C, Irvine RF (2001). "Inositol 1, ... Initial studies determined that amino acids 9-52 from the rat ITPKA were useful as a live-cell reporter for actin filaments. ... Melak M, Plessner M, Grosse R (2017). "Actin visualization at a glance". J Cell Sci. 130 (3): 525-530. doi:10.1242/jcs.189068. ...
... of four members of the POU family of proteins in activated and phorbol 12-myristate 13-acetate-treated Jurkat T cells". ... retinal neurons called ganglion cells, and in cells of the B- and T-lymphocytic lineages. Brn3a was initially discovered in ... "Regulation of Hsp27 expression and cell survival by the POU transcription factor Brn3a". Cell Death and Differentiation. 11 (11 ... Cytogenetics and Cell Genetics. 74 (3): 225-6. doi:10.1159/000134422. PMID 8941380. Smith MD, Dawson SJ, Latchman DS (Jan 1997 ...
... product of the c-cbl protooncogene is the 120-kDa tyrosine-phosphorylated protein in Jurkat cells activated via the T cell ... 1995). "Reconstitution of the B cell antigen receptor signaling components in COS cells". J. Biol. Chem. 270 (45): 27072-8. doi ... Cell. Biol. 13 (9): 5877-87. doi:10.1128/MCB.13.9.5877. PMC 360336. PMID 8395016. Malek SN, Yang CH, Earnshaw WC, et al. (1996 ... Cell. Biol. 16 (9): 4735-43. doi:10.1128/MCB.16.9.4735. PMC 231474. PMID 8756631. Saouaf SJ, Wolven A, Resh MD, Bolen JB (1997 ...
Hsp60 and Hsp10 in the mitochondrial fraction of jurkat cells". EMBO J. 18 (8): 2040-8. doi:10.1093/emboj/18.8.2040. PMC ... Infection and disease are extremely stressful on the cell. When a cell is under stress, it naturally increases the production ... HSP60 has been shown to be released from specific cells like peripheral blood mononuclear cells (PBMCs) when there are ... March 2007). "Heat shock protein 60: regulatory role on innate immune cells". Cell. Mol. Life Sci. 64 (6): 742-51. doi:10.1007/ ...
... type-1 Tat protein selectively stimulates a phosphatidylinositol-specific phospholipase C nuclear pathway in the Jurkat T cell ... doi:10.1016/j.cell.2006.09.026. PMID 17081983. S2CID 7827573. Biology portal v t e. ... 1991). "Human immunodeficiency virus-1 glycoproteins gp120 and gp160 specifically inhibit the CD3/T cell-antigen receptor ... 2006). "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks". Cell. 127 (3): 635-48. ...
Cells that expressed no signs of cytopathy from SFV were the Jurkat and Hut-78 T-cell lines. The phylogenetic tree analysis of ... SFV can infect a wide range of cells, with in vitro experiments confirming that fibroblasts, epithelial cells, and neural cells ... SFV causes cells to fuse with each other to form syncytia, whereby the cell becomes multi-nucleated and many vacuoles form, ... As the cell transcribes the integrated proviral genome, glycoproteins are produced and displayed at the surface of the cell. If ...
"Protein-tyrosine kinase Pyk2 is involved in interleukin-2 production by Jurkat T cells via its tyrosine 402". J. Biol. Chem. ... interacts with nephrocystin and both proteins localize to cell-cell contacts of polarized epithelial cells". Exp. Cell Res. 256 ... Fyn's normal role in cell migration and adhesion enables it to utilize the normal cell biology of integrin and FAK for cancer ... Normal integrin is a cell surface receptor that interacts with the extracellular matrix to send signals influencing cell shape ...
... expression of DHX32 in Jurkat T cells is specific and involves calcium and nuclear factor of activated T cells". Cell. Immunol ... Liu J, Yuan Y, Huan J, Shen Z (2001). "Inhibition of breast and brain cancer cell growth by BCCIPalpha, an evolutionarily ... 2007). "A role for DHX32 in regulating T-cell apoptosis". Anticancer Res. 27 (1A): 373-7. PMID 17352256. Chen Y, Alli Z, ...
Hsp60 and Hsp10 in the mitochondrial fraction of jurkat cells". EMBO J. 18 (8): 2040-8. doi:10.1093/emboj/18.8.2040. PMC ... Hsp60 and Hsp10 in the mitochondrial fraction of jurkat cells". EMBO J. 18 (8): 2040-8. doi:10.1093/emboj/18.8.2040. PMC ... Cell. Cardiol. 35 (9): 1135-43. doi:10.1016/S0022-2828(03)00229-3. PMID 12967636. Shan YX, Yang TL, Mestril R, Wang PH (2003 ... Cell Mol. Genet. 24 (6): 315-26. doi:10.1023/A:1024488422990. PMID 10763410. S2CID 39860709. Richardson A, Schwager F, Landry ...
... kb cells MeSH A11.251.210.190.465 - hl-60 cells MeSH A11.251.210.190.475 - ht29 cells MeSH A11.251.210.190.495 - jurkat cells ... kb cells MeSH A11.251.860.180.465 - hl-60 cells MeSH A11.251.860.180.475 - ht29 cells MeSH A11.251.860.180.495 - jurkat cells ... cho cells MeSH A11.251.210.505 - l cells (cell line) MeSH A11.251.210.520 - llc-pk1 cells MeSH A11.251.210.700 - 3t3 cells MeSH ... l cells MeSH A11.329.228.900 - 3t3 cells MeSH A11.329.228.900.080 - balb 3t3 cells MeSH A11.329.228.900.550 - nih 3t3 cells ...
... jurkat cells MeSH A15.382.490.555.567.569.500 - t-lymphocyte subsets MeSH A15.382.490.555.567.622 - lymphocytes, null MeSH ... foam cells MeSH A15.382.680.397.376 - giant cells, foreign-body MeSH A15.382.680.397.380 - giant cells, langhans MeSH A15.382. ... foam cells MeSH A15.382.812.522.376 - giant cells, foreign-body MeSH A15.382.812.522.380 - giant cells, langhans MeSH A15.382. ... killer cells MeSH A15.145.229.637.555.567.537 - killer cells, natural MeSH A15.145.229.637.555.567.537.500 - killer cells, ...
HEK 293 cells - derived from human fetal cells. Jurkat cells - a human T lymphocyte cell line isolated from a case of leukemia ... A549 cells - derived from a cancer patient lung tumor. HeLa cells - a widely used human cell line isolated from cervical cancer ... Vero cells - a monkey kidney cell line that arose by spontaneous immortalisation. List of breast cancer cell lines Skloot R ( ... Immortalised cell lines have also found uses in biotechnology. An immortalised cell line should not be confused with stem cells ...
Jurkat cells are an immortalized line of human T lymphocyte cells that are used to study acute T cell leukemia, T cell ... Jurkat Cells at the US National Library of Medicine Medical Subject Headings (MeSH) Cellosaurus entry for Jurkat. ... Different derivatives of the Jurkat cell line that have been mutated to lack certain genes can now be obtained from cell ... Abraham, Robert; Weiss, Arthur (2004). "Jurkat T cells and development of the T-cell receptor signalling paradigm". Nature. 4 ( ...
... jurkat cells include A Chromatin Immunoprecipitation Assay to Identify Novel NFAT2 Target Genes in Chronic Lymphocytic ... Visualizing Cell-to-cell Transfer of HIV using Fluorescent Clones of HIV and Live Confocal Microscopy, Methods to Assess Beta ... Cell Death Mediated by Cytotoxic T Lymphocytes, Assessing Autophagic Flux by Measuring LC3, p62, and LAMP1 Co-localization ... Leukemia, Automated Counterflow Centrifugal System for Small-Scale Cell Processing, Imaging the Human Immunological Synapse ...
Human Acute T Cell Leukemia) whole blood lymph cell lysate 2mg/mL supplied in SDS sample buffer. Shop Novus Biologicals™ Jurkat ... Cell Culture & Analysis Cell Culture & Analysis * Cell Culture Dishes, Plates and Flasks ... Jurkat, clone E6-1 (Human Acute T Cell Leukemia) whole blood lymph cell lysate 2mg/mL supplied in SDS sample buffer containing ... The Jurkat Whole Cell Lysate is derived from human. It has been validated for the following applications: Western Blot. ...
... posted in Tissue and Cell Culture: Hi How can I do to separate live and dead Jurkat cells.? I recently thawed this cell line. I ... counted with trypan blue and saw many dead cells. Please, help me !!! I do not want to lose these cells. ... LPS-stimulation of Jurkat T-cells to induce TNF-alpha Started by TZ89, 11 Jun 2013 LPS, Jurkat, TNF, T-cell * 0 replies ... Stimulating Jurkat T Cells with PMA: Clumping Cells? Started by chuggleschuggles, 23 Oct 2014 jurkat, stimulation, pma and 1 ...
Lysophospholipids have recently been demonstrated to induce activation and proliferation of fibroblasts and other cell lineages ... by interacting with high affinity cell surface receptors leading to specific intracellular signaling events. Platelet ... Effect of lysophospholipids on signaling in the human Jurkat T cell line J Cell Physiol. 1995 Jun;163(3):441-50. doi: 10.1002/ ... In addition, LPA also increased the production of the T cell growth factor, interleukin 2 (IL-2), by Jurkat cells treated with ...
... constituents present in ethanolic extracts of Echinacea species exert direct immunomodulatory effects on human Jurkat T cells. ... Echinacea alkylamides inhibit interleukin-2 production by Jurkat T cells Alkylamides present in Echinacea species have reported ... Thus, alkylamides present in E. purpurea suppress the ability of activated Jurkat T cells to produce IL-2 independently of ... Modulation of IL-2 production by submaximally stimulated Jurkat cells was determined in response to treatment with extracts ...
The Jurkat cell line is derived from human T-cell leukemia and used to determine the mechanism of differential susceptibility ... Transmission electron micrograph of a Jurkat T-cell in the early stages of apoptosis, with apoptotic bodies forming. ... The Jurkat cell line is derived from human T-cell leukemia and used to determine the mechanism of differential susceptibility ... Credit: TEM Jurkat T cell early apoptopsis. Credit: Dr Jeremy Skepper. Attribution 4.0 International (CC BY 4.0) ...
The Jurkat T lymphoma line has certain advantage over primary cells for studying basic cell biological processes such as IS ... Cells and Reagents.. Jurkat T cells were maintained and transfected as described previously (16). All lipids were purchased ... Mechanisms for segregating T cell receptor and adhesion molecules during immunological synapse formation in Jurkat T cells. ... Mechanisms for segregating T cell receptor and adhesion molecules during immunological synapse formation in Jurkat T cells ...
The incorporation properties of ceramide analogues for click chemistry in Jurkat T cells were investigated. The analogues ... Incorporation studies of clickable ceramides in Jurkat cell plasma membranes T. Walter, J. Schlegel, A. Burgert, A. Kurz, J. ... The incorporation properties of ceramide analogues for click chemistry in Jurkat T cells were investigated. The analogues ...
The images of the Jurkat cell under osmotic change were processed to obtain a relationship between cell volume change and time ... using a novel microfluidic controlled single cell-trapping system. The osmotic behavior of an individual Jurkat cell to water ... The experimental results were fitted using a two-parameter transport numeric model to calculate the Jurkat cell membrane ... In this study we propose a comprehensive process for determination of membrane transport properties of Jurkat cell. ...
Jurkat cells are an immortalized T-lymphocyte cell line, treated with serial dilutions of staurosporine, camptothecin, and ... Jurkat cells are an immortalized T-lymphocyte cell line. They have been used to study T-cell leukemia, T-cell signaling, and ... Jurkat cells seeded at densities ranging from 390 to 50,000 cells per well were counted using StainFree technology (blue dots ... Jurkat cells were imaged using the SpectraMax MiniMax 300 Imaging Cytometer, and cells were identified using the predefined ...
... posted in Tissue and Cell Culture: Hello I have transfected Jurkat cells with a plasmid contating my gene and resistance to ... I want to perform limiting dilution in order to obtain a cell population from a single clone. Does somebody has a protocol, ... advice or tips? I mean things like: G418 concentration, cell concentration or any other special conditions. Thank you, Roman ... Protocols and Techniques Forums → Tissue and Cell Culture → Jurkat cell Started by carolfrozza, 09 Apr 2014 jurkat * 4 replies ...
... leukemic T-cell lymphoblast), 88042803; find null-null MSDS, related peer-reviewed papers, technical documents, similar ... Jurkat E6.1 Cell Line human from human blood( ... Jurkat E6.1 Cell Line human from human blood(leukemic T-cell ... Cell Line Origin Human leukaemic T cell lymphoblast Cell Line Description Derived from Jurkat FHCRC. An IL-2 producing cell ... Jurkat E6.1 cell line has been used to:. • study cell-independent/vector-f. ree delivery of bovine serum albumin-fluorescein ...
NFAT-CD16 cells are human reporter cells for the early nuclear translocation of NFAT upon antibody-dependent cellular ... ADCC reporter T-cell line. Jurkat-Lucia™ NFAT-CD16 cells were engineered from the human T-Lymphocyte Jurkat cell line. Jurkat ... Raji-hCTLA4 Cells Human lymphoblast cells - ADCC CTLA-4 Target Cells Raji-hPD-1 Cells Human lymphoblast cells - ADCC PD-1 ... Jurkat-Lucia™ NFAT-CD16 Cells. Jurkat-Lucia™ NFAT-CD16 Cells. Unit size. Cat. code. Docs. Qty. Price. ...
Jurkat cells transfected with IL-8R1 or IL-8R2 migrate in response to IL-8, GRO alpha and NAP-2.. Loetscher P1, Seitz M, Clark- ... By cDNA transfection Jurkat cell lines were generated that stably express either IL-8R1 or IL-8R2 (J-IL8R1 and J-IL8R2). J- ... Neutrophil leukocytes, the target cells for interleukin-8 and related CXC chemokines, bear high numbers of two types of IL-8 ...
... human acute T cell leukemia induced with Calyculin A) Whole Cell Lysate is provided as a Western blotting positive control ... Jurkat + Calyculin A Cell Lysate whole cell lysate provided as Western blotting positive control *Home. ... human whole cell lysate; acute T cell leukemia cells induced with Calyculin A ... Jurkat + Calyculin A Cell Lysate: sc-2277 *bvseo_sdk, java_sdk, bvseo-3.2.0 ...
... we conducted a genome-wide functional knockout screen using CRISPR-Cas9 in a HIV-1 latency cell line model. This screen ... CRISPR-based gene knockout screens reveal deubiquitinases involved in HIV-1 latency in two Jurkat cell models Sci Rep. 2020 Mar ... we conducted a genome-wide functional knockout screen using CRISPR-Cas9 in a HIV-1 latency cell line model. This screen ...
Antiproliferative and apoptotic effects of pterostilbene were examined in combination with L-asparaginase in Jurkat cell line. ... Cell culture:. Jurkat cells were grown in RPMI 1640 supplemented with 0.3 mg/ml L-glutamine, 10 % fetal calf serum, 100 IU/ml ... Jurkat cells were seeded in a concentration of 1×104 cells per well in a 96 well plate and incubated with different ... Figure 1: Effect of pterostilbene on Jurkat cell viability. a period of 24, 48 and 72 h. Controls are the cells incubated with ...
Detailed Analysis of Apoptosis and Delayed Luminescence of Human Leukemia Jurkat T Cells after Proton Irradiation and ... "Detailed Analysis of Apoptosis and Delayed Luminescence of Human Leukemia Jurkat T Cells after Proton Irradiation and ...
The present study examined the effects of Embelin on the proliferation of human acute T cell lymphoma Jurkat cells. Jurkat ... Effect of Embelin on the proliferation of Jurkat cells. Viability represents the percentage of viable Jurkat cells measured ... Inhibitory effect of Embelin on human acute T cell lymphoma Jurkat cells through activation of the apoptotic pathway.. Zhu XL1 ... The results showed that Embelin significantly inhibited the growth of human acute T cell lymphoma Jurkat cells. Following ...
Detailed Analysis of Apoptosis and Delayed Luminescence of Human Leukemia Jurkat T Cells after Proton Irradiation and ... 62 MeV proton beams were used to irradiate cells under a uniform dose of 2 or 10 Gy, respectively. We assessed apoptosis, cell ... in human leukemia Jurkat T cells under a wide variety of treatments. We used menadione and hydrogen peroxide to induce ...
Temporal changes in cell shape and F-actin morphology were characterized in Jurkat cells attached by immobilized anti-CD3 ... Accordingly, we have characterized changes in cell shape and F-actin morphology occurring in the Jurkat T cell leukemia ... Actin polymerization and pseudopod reorganization accompany anti-CD3-induced growth arrest in Jurkat T cells.. M V Parsey and G ... Jurkat cells attached by either stimulatory or nonstimulatory antibodies reorganized their actin similarly after the first ...
Actin polymerization and pseudopod reorganization accompany anti-CD3-induced growth arrest in Jurkat T cells.. M V Parsey and G ... Actin polymerization and pseudopod reorganization accompany anti-CD3-induced growth arrest in Jurkat T cells. ... Actin polymerization and pseudopod reorganization accompany anti-CD3-induced growth arrest in Jurkat T cells. ... Actin polymerization and pseudopod reorganization accompany anti-CD3-induced growth arrest in Jurkat T cells. ...
Stimulation of CD28 triggers an association between CD28 and phosphatidylinositol 3-kinase in Jurkat T cells.. K E Truitt, C M ... The T cell surface molecule CD28 can provide costimulatory signals that permit the full activation of T cells. Here we ... When added to lysates of B7-stimulated Jurkat cells, the oligopeptide corresponding to Tyr 173 inhibits the ... Stimulation of CD28 triggers an association between CD28 and phosphatidylinositol 3-kinase in Jurkat T cells. ...
HNRNPD is strongly supported by BioGPS gene expression data to be expressed in Human Jurkat cells , Alias: P37; AUF1; AUF1A; ... 293/Jurkat/3T3/COLO205 cells. HNRNPD Antibody (OAAF01114). Application: WB, IHC, IF, ELISA. Species: Human, Mouse. ... Product Protocols: HNRPD antibody tested with Human Jurkat Cells (ARP40238_T100). Product Protocols: HNRPD antibody tested by ... and GRHL2 in human oral squamous cell carcinoma cells. Oncogene 28, 565-74 (2009). IHC, WB, Cow, Dog, Guinea Pig, Horse, Human ...
ENO1 is strongly supported by BioGPS gene expression data to be expressed in Human Jurkat cells , Alias: NNE; PPH; MPB1; ENO1L1 ... Find tissues and cell lines supported by DNA array analysis to express ENO1. ... Find tissues and cell lines supported by RNA-seq analysis to express ENO1. ... ENO1 is strongly supported by BioGPS gene expression data to be expressed in Jurkat ...
... in acute lymphoblastic leukemia Jurkat cells and to characterize the underlying ... Role of epigenetic regulation on the induction of apoptosis in Jurkat leukemia cells by white grape pomace rich in phenolic ... Role of epigenetic regulation on the induction of apoptosis in Jurkat leukemia cells by white grape pomace rich in phenolic ... PWGPE induced apoptosis in Jurkat cells in a time- and concentration-dependent manner. The anticancer effect was associated ...
... Samali A. ... In the present study, we demonstrate that pro-caspase-3 is present in the mitochondrial fraction of Jurkat T cells in a complex ... finding suggests that the release of mitochondrial Hsps may also accelerate caspase activation in the cytoplasm of intact cells ...
Jurkat cells (2 × 106 cells/ml) were tethered onto different GlyCAM-1/IgG chimer ... Comparison of Jurkat cell rolling velocities on various GlyCAM-1/IgG chimeras. ... Figure 3: Comparison of Jurkat cell rolling velocities on various GlyCAM-1/IgG chimeras. Jurkat cells (2 × 106 cells/ml) were ... Figure 3: Comparison of Jurkat cell rolling velocities on various GlyCAM-1/IgG chimeras. Jurkat cells (2 × 106 cells/ml) were ...
Regulation of biochemical and morphological features of chemical- and receptor-mediated apoptosis in Jurkat T cells ... Using the MCF-7 cell line, it was found that caspase-3 and DFF40/45 were dispensable for the formation of HMW DNA fragments. ...
  • Transmission electron micrograph of a Jurkat T-cell in the early stages of apoptosis, with apoptotic bodies forming. (wellcomecollection.org)
  • Antiproliferative and apoptotic effects of pterostilbene were examined in combination with L-asparaginase in Jurkat cell line. (ijpsonline.com)
  • Pterostilbene increased antiproliferative and apoptotic effects of L-asparaginase in Jurkat cells. (ijpsonline.com)
  • Inhibitory effect of Embelin on human acute T cell lymphoma Jurkat cells through activation of the apoptotic pathway. (nih.gov)
  • It was also shown that the apoptotic rate of cells treated with Embelin was significantly elevated. (nih.gov)
  • These results showed that Embelin inhibited growth and induced apoptosis of Jurkat cells in vitro , by activating the endogenous caspase-dependent apoptotic pathway through inhibition of XIAP and proapoptotic Bcl-2 family members. (nih.gov)
  • Left upper, right upper, left lower and right lower quadrants represent necrotic cells, late apoptotic cells, healthy cells and early apoptotic cells, respectively. (nih.gov)
  • Presence of a pre-apoptotic complex of pro-caspase-3, Hsp60 and Hsp10 in the mitochondrial fraction of jurkat cells. (uniprot.org)
  • Clear decreases of the B-CI correlated with the presence of a large number of apoptotic cells as observed by microscopy (data not shown). (nih.gov)
  • 2-Amino-2-[2-(4-octylphenyl)ethyl] propane-1,3-diol hydrochloride (FTY720), a synthetic product derived from a metabolite of Isaria sinclairii , has been demonstrated to have a potent immunosuppressive activity that induces apoptotic cell death in T cells and several other cell lines. (aspetjournals.org)
  • In this study, using the human T-lymphoma cell line, Jurkat cells, we investigated the apoptotic signal transduction mediated by FTY720, in particular comparing its role on the cleavage of caspases, with that mediated by etoposide or anti-Fas antibody. (aspetjournals.org)
  • Pretreatment with a broad caspase inhibitor [benzyloxycarbonyl-Val-Ala-Asp-(Ome) fluoromethyl ketone] markedly decreased the incidence of apoptotic cells induced by FTY720, etoposide, and anti-Fas antibody, through the abrogation of cleavage of Bid, poly(ADP-ribose) polymerase, and caspases 3, 8, and 9. (aspetjournals.org)
  • These agents enhanced cell shrinkage and apoptotic bodies formation induced by FasL, as well as the cleavage of the executor caspases 3 and 7 and of its substrates PARP and α-fodrin. (aacrjournals.org)
  • This chemical selectivity, together with the lack of apoptotic activity against rat Leydig cells, argues against a general cell poisoning effect. (aspetjournals.org)
  • The apoptotic induction seems independent of the mitochondrial peripheral-type benzodiazepine receptor (PBR) that binds these pyrrolobenzoxazepines with high affinity, due to the lack of correlation between their affinities for the receptor and their apoptotic potencies, their high apoptotic activity in PBR-deficient cells such as Jurkats, and their lack of apoptotic induction in PBR-rich rat Leydig cells. (aspetjournals.org)
  • Although the mechanisms by which MG triggers apoptotic events are not fully understood, our previous studies have suggested that intense activation of the JNK pathway triggered by MG contributes to mitochondrial dysfunction and subsequent cell death (Du et al. (aspetjournals.org)
  • To study the effect of 1 h intermittent exposure to 50 Hz electromagnetic field ( power transmission line signal ) on Jurkat cells by evaluating the reactive oxygen species production and apoptosis , both spontaneous and induced by the well kown pro- apoptotic agent, anti-Fas (50 ng/ml). (emf-portal.org)
  • Jurkat cells were employed because it has been reported that treatment with anti-Fas resulted in proteolytic cleavage of precursors of caspase -3, thus triggering an apoptotic process. (emf-portal.org)
  • Mitochondrial ROS Release and Subsequent Akt Activation Potentially Mediated the Anti-Apoptotic Effect of a 50-Hz Magnetic Field on FL Cells. (emf-portal.org)
  • To characterize the mechanisms involved in human fetal retinal pigment epithelium (HFRPE) induced apoptosis in human T-cells, we analyzed the caspase cascade in apoptotic Jurkat T-cells (Jkt) which were incubated with supernatant of HFRPE cells. (arvojournals.org)
  • These results suggest TAL-1 influences expression of proteins involved in the NF-kB signaling pathway, thus inducing an anti-apoptotic response in the cell. (bsu.edu)
  • The antiproliferative effect of ZER on Jurkat cells was through the apoptotic intrinsic pathway via the activation of caspase-3 and -9. (upm.edu.my)
  • Apoptotic cell death induced by the ethanolic extracts at 1 × IC 50 and 2 × IC 50 concentrations was demonstrated by DAPI staining, gel electrophoresis, flow cytometry with Annexin V/propidium iodide staining, and caspase-3, -8, and -9 enzyme activities. (biomedcentral.com)
  • The six active extracts preferentially induced apoptotic cell death in a concentration-dependent manner with DNA fragmentation (2 × IC 50 ). (biomedcentral.com)
  • Simultaneous measurement of secreted IL-2 by ELISA and cell viability by the XTT assay showed that the 95:5 ethanol/water extract of E. purpurea was both IL-2 suppressive and cytotoxic at 50 and 100 microg/mL. (bastyr.edu)
  • Jurkat cells seeded at densities ranging from 390 to 50,000 cells per well were counted using StainFree technology (blue dots), or they were stained with EarlyTox™ Live Cell Assay dye and green fluorescent cells were counted (green dots). (moleculardevices.com)
  • Jurkat cells were treated with serial dilutions of staurosporine (red plot), camptothecin (green plot), and etoposide (blue plot) for 24 hours and then assayed using Molecular Devices EarlyTox Live/Dead Assay Kit. (moleculardevices.com)
  • Jurkat cells were treated with staurosporine (red plot), camptothecin (green plot), or etoposide (blue plot) for 28 hours and then assayed for apoptosis using the EarlyTox Caspase-3/7 NucView 488 Assay Kit. (moleculardevices.com)
  • Jurkat-Lucia™ NFAT-CD16 cells have been designed as effector reporter cells for InvivoGen's ADCC assay. (invivogen.com)
  • Cell viability was evaluated using 3-(4,5-dimethylthiazol-2-yl)-5-3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2htetrazolium assay. (ijpsonline.com)
  • Viability represents the percentage of viable Jurkat cells measured using an MTS assay. (nih.gov)
  • Drug cytotoxicity monitoring of Jurkat/L1236 cells in the presence/absence of Fibronectin: RTCA vs MTT assay.Notes: (A) We incubated Jurkat/L1236 cells (treatment point [+]) in the presence of trabectedin (T) (10 nM) + FasL (50 ng/mL), bendamustine (200 μM), oxaliplatin (100 μM), trabectedin (10 nM) + FasL (50 ng/mL), or bendamustine + oxaliplatin (up to 24 hours). (nih.gov)
  • Trypan blue exclusion assay, MTS assay and FACS analysis were carried out to analyze cell viability, cell proliferation and apoptosis, respectively. (academicjournals.org)
  • Frozen cells should be thawed immediately upon receipt and grown according to handling procedure to ensure cell viability and proper assay performance. (creative-biolabs.com)
  • Ca2+ -flux inhibition fluorescence assay in Jurkat cells is useful 384-plate format HTS screening assay for quick identification of STIM1/ORAI1 inhibitory compounds what is representative for CRAC inhibition in T cells. (fidelta.eu)
  • This investigation determined the anticancer properties of zerumbone (ZER) on the human T-cell (Jurkat) line using the MTT assay, microscopic evaluations, flow cytometric analyses, and caspase activity estimations. (upm.edu.my)
  • Cell viability was determined by trypan blue assay. (exp-oncology.com.ua)
  • Conventional methods for evaluating cell culturing techniques and assay design consist of manual inspection of a small subset of the cell population at random locations and time points. (biotekinstruments.ru)
  • Diverse assay formats and reagents have been developed that measure specific aspects of cell viability corresponding to particular cellular response pathways and mechanisms of injury. (biotekinstruments.ru)
  • Glucose uptake was evaluated in HeLa cells using BioVision's GluTracker™ Assay Kit (A) or BioVision's AGTracker™ Assay Kit (B). Cells were treated with or without 1X phloretin for 45 minutes. (news-medical.net)
  • Additionally, the proliferation and apoptosis of Hut-78 cells exposed to different concentration of Adriamycin (ADM) in normoxia and hypoxia were evaluated by MTT and Annexin-V FITC/PI staining assay. (springer.com)
  • Finally, the effects of AEG-1 on Hut-78 cells exposed to ADM in hypoxia were assessed by MTT and Annexin-V FITC/PI staining assay, and 3-MA (autophagy inhibitor) was further used to determine the underlying mechanism. (springer.com)
  • J.RT3-T3.5 cells have a mutation in the T cell receptor beta chain locus precluding expression of this chain. (wikipedia.org)
  • The TCR-peptide-major histocompatibility complex (MHC), cytoplasmic signaling proteins, and adhesion molecules [e.g., the integrin leukocyte function-associated antigen (LFA)-1 on the T cell and its ligand, intercellular adhesion molecule (ICAM)-1, on the APC] concentrate at the cell-cell interface and segregate into a distinctive structure known as the immunological synapse (IS) ( 1 , 2 ). (pnas.org)
  • Jurkat cells are an immortalized line of human T lymphocyte cells that are used to study acute T cell leukemia, T cell signaling, and the expression of various chemokine receptors susceptible to viral entry, particularly HIV. (wikipedia.org)
  • The Jurkat cell line (originally called JM) was established in the late 1970s from the peripheral blood of a 14-year-old boy with T cell leukemia. (wikipedia.org)
  • Different derivatives of the Jurkat cell line that have been mutated to lack certain genes can now be obtained from cell culture banks. (wikipedia.org)
  • The JCaM1.6 cell line is deficient in Lck activity due to the deletion of part of the LCK gene (exon 7) from the LCK transcript. (wikipedia.org)
  • The I 2.1 cell line is functionally defective for FADD and the I 9.2 cell line is functionally defective for caspase-8, both defective molecules being essential to apoptosis or necroptosis of cells. (wikipedia.org)
  • The D1.1 cell line does not express the CD4 molecule, an important co-receptor in the activation pathway of helper T cells. (wikipedia.org)
  • a clone of the Jurkat-FHCRC cell line. (thermofisher.com)
  • I recently thawed this cell line. (protocol-online.org)
  • The Jurkat cell line is derived from human T-cell leukemia and used to determine the mechanism of differential susceptibility to anti-cancer drugs and radiation. (wellcomecollection.org)
  • The Jurkat cell is an immortalized line of human acute lymphocyte leukemia cells that is widely used in the study of adoptive cell therapy, a novel treatment of several advanced forms of cancer. (mdpi.com)
  • Established in the late 1970s from the peripheral blood of a young boy with T-cell leukemia, Jurkat cells are an immortalized T-lymphocyte cell line. (moleculardevices.com)
  • An IL-2 producing cell line, derived by incubating the cells at 41°C for 48 hours followed by a limiting dilution cloning over macrophages. (sigmaaldrich.com)
  • Jurkat-Lucia™ NFAT-CD16 cells were engineered from the human T-Lymphocyte Jurkat cell line. (invivogen.com)
  • Induction of antibody-dependent cellular cytotoxicity (ADCC) has been validated using InvivoGen's anti-hCD20-hIgG1 antibody and Raji-Null target cell line. (invivogen.com)
  • To investigate host factors which promote HIV-1 latency, we conducted a genome-wide functional knockout screen using CRISPR-Cas9 in a HIV-1 latency cell line model. (nih.gov)
  • Unravelling the mechanism of pterostilbene-induced cell apoptosis in this cell line could help in the development of a targeted therapy. (ijpsonline.com)
  • Using the MCF-7 cell line, it was found that caspase-3 and DFF40/45 were dispensable for the formation of HMW DNA fragments. (bl.uk)
  • In this work, we studied the relationship between the reduction of intracellular GSH concentration, intracellular K + loss and the progression of apoptosis induced by Fas ligand (FasL) in the Jurkat leukemia cell line. (aacrjournals.org)
  • Assuming that the Jurkat cell line is representative of normal cycling human T lymphocytes, we conclude that the presence of the CD2 molecule on the plasma membrane is not in itself a requirement for an operational CD3-Ti-alpha/beta receptor. (rupress.org)
  • Extracts were added to the cultured cells of selected cell line in various concentrations (10, 25, 50, 75 and 100 µg/ml) and incubated for 24 h. (academicjournals.org)
  • Results of this study demonstrate that n-hexane extract of C. intybus has potent anti-proliferative and cytotoxic activity against Jurkat cells, a human leukemia cell line. (academicjournals.org)
  • The anti-MAGEA3 (EVDPIGHLY) TCR (clone MAG-IC3) Jurkat cell line is a stable cell line made from the anti-MAGEA3 TCR lentivirus. (creative-biolabs.com)
  • The Jurkat cell line was established from the peripheral blood of human T lymphocyte cells. (creative-biolabs.com)
  • The cell line has been screened using the luciferase based mycoplasma detection kit to confirm the absence of mycoplasma species. (creative-biolabs.com)
  • Note: Do not freeze the cells upon receipt as it may result in irreversible damage to the cell line. (creative-biolabs.com)
  • The cell line has been screened using the membrane filtration testing methods to confirm the absence of aerobic, anaerobic and fungi microorganisms. (creative-biolabs.com)
  • Isoenzyme analysis is used to confirm the identity of the species of a cell line. (creative-biolabs.com)
  • The anti-MCC peptide (ADLIAYLKQATK) TCR (clone 226) Jurkat cell line is a stable cell line made from the anti-MCC peptide TCR lentivirus. (creative-biolabs.com)
  • A novel aspect of T-cell membrane activation studied with a Jurkat tumour cell line. (harvard.edu)
  • Using an in vivo cross-linking strategy, specialized genomic sequences (0.1-1.1 kbp) that bind to SATB1 in human lymphoblastic cell line Jurkat cells were individually isolated and characterized. (ovid.com)
  • In the wild type Jurkat cell line as well as in CD3- and CD2- variants, AlF4- can activate the G protein(s) presumably associated with phospholipase C to generate polyphosphoinositide turnover as well as an increase in cytoplasmic free calcium ions. (pasteur.fr)
  • Hence, the data indicate that periplasmic PP14 can mediate IL-2 suppression and that the suppressive effect can be monitored in a T cell line. (elsevier.com)
  • Extremely low-frequency electromagnetic field induces a change in proliferative capacity and redox homeostasis of human lung fibroblast cell line MRC-5. (emf-portal.org)
  • Effect of extremely low-frequency electromagnetic fields on antioxidant activity in the human keratinocyte cell line NCTC 2544. (emf-portal.org)
  • The untreated, Tat-expressing cell lines had lower levels of peroxiredoxin compared to the parental Jurkat E6.1 T cell line. (biomedcentral.com)
  • The HIV Tat-expressing cell lines showed greater levels of oxidative stress than the Jurkat E6.1 cell line when treated with SMX-HA. (biomedcentral.com)
  • Ectopic expression of Cdc42 GTPase robustly rescued the CE cell movement defects in both def6aqmc811/qmc811 and swap70bqmc809/qmc809 homozygous mutant lines whereas ectopic expression of RacI robustly rescued CE cell movement defects only in swap70bqmc809/qmc809 homozygous mutant line, suggesting that Def6a and Swap70b acts upstream of Cdc42 and Cdc42/RacI respectively. (nottingham.ac.uk)
  • Jurkats are an IL-2 producing T lymphocyte cell line, commonly used to study T cell signaling. (tgrbio.com)
  • This cell line has been screened using the MycoAlert™ Mycoplasma Detection Kit (Lonza, #LT07-118) to confirm the absence of Mycoplasma contamination. (bpsbioscience.com)
  • Purchase of this cell line grants you with a 10-year license to use this cell line in your immediate laboratory, for research use only. (bpsbioscience.com)
  • The license does not permit the use of this cell line in humans or for therapeutic or drug use. (bpsbioscience.com)
  • The license does not permit modification of the cell line in any way. (bpsbioscience.com)
  • Inappropriate use or distribution of this cell line will result in revocation of the license and result in an immediate cease of sales and distribution of BPS products to your laboratory. (bpsbioscience.com)
  • BPS does not warrant the suitability of the cell line for any particular use, and does not accept any liability in connection with the handling or use of the cell line. (bpsbioscience.com)
  • Publications using this cell line should reference BPS Bioscience, Inc., San Diego. (bpsbioscience.com)
  • Following selection the Jukat NucLight Red Cells were validated by comparing morphology and chemotactic migration profiles to the parental cell line. (essenbioscience.com)
  • Cell line: Jurkat (Human T cell lymphoblast-like). (abcam.com)
  • Often this cell line is called 'JM' (Jurkat and JM are derived from the same patient and are sister clones), occasionally JM may be a subclone with somewhat divergent features. (abcam.com)
  • Normal and interferon (IFN)-γ-activated HFRPE from early and late in vitro passages were incubated with cells from the human T-cell leukemia line Jurkat (Jkt). (umn.edu)
  • The role of Fas ligand (FasL) molecule in HFRPE-mediated apoptosis was assessed by using a mutant Jkt cell line (DD3), which is deficient in Fas-mediated signaling. (umn.edu)
  • A marked and immediate tyrosine phosphorylation was induced in the leukemic T cell line, Jurkat. (elsevier.com)
  • The purpose of this study was to determine the role of radiation-induced expression of c-jun and c-fos in radiation-induced apoptosis of cells of the Jurkat T-cell line. (allenpress.com)
  • To measure nitric oxide (NO) production in the form of nitrite derivative in relation to cell viability and apoptosis development in human peripheral blood mononuclear cells compared to that processes in human leukemic Jurkat T-cell line. (exp-oncology.com.ua)
  • Their effect was better expressed in Jurkat T-cell line. (exp-oncology.com.ua)
  • Strong positive correlation was demonstrated between NO production and apoptosis development in both studied cell types, however leukemic Jurkat T-cell line responses were better expressed than such responses in normal mononuclear cells of peripheral blood. (exp-oncology.com.ua)
  • Human Acute T-Cell Leukemic Cell Line (Clone E61). (bpsbioscience.com)
  • This cell line has been screened using the MycoAlert™ Mycoplasma Detection Kit (Cat. (bpsbioscience.com)
  • The NF-ĸB-luciferase/Jurkat cell line is suitable for monitoring the activity of NF-ĸB signaling in response to stimulants, and establishing cell-based screens for inhibitors that target specific NF- ĸB-stimulating molecules. (bpsbioscience.com)
  • Therefore, we examined the effects of 1α,25(OH) 2 D 3 on the NF-κB pathway in the Jurkat cell line, a human T cell line that constitutively expresses endogenous vitamin D receptor. (elsevier.com)
  • For further information on this cell line and other parameters, including different strains, vendors, implant type and location and/or standards of care, please contact Covance. (covance.com)
  • To provide a more rational basis for clinical combinations with another commonly used drug, cisplatin, we assessed the modulation of MGMT protein and mRNA levels in the human leukemic cell line Jurkat after treatment with these agents. (openrepository.com)
  • Chemoresistance to temozolomide in human glioma cell line U251 is associated with increased activity of O6-methylguanine-DNA methyltransferase and can be overcome by metronomic temozolomide regimen. (openrepository.com)
  • Using a third-generation lentiviral system to express HCV-core in CD4 + Jurkat T-cells, we describe that HCV-core-expressing Jurkat cells show an up-regulation of FOXP3 (forkhead box P3) and CTLA-4 (cytotoxic T-lymphocyte antigen-4). (portlandpress.com)
  • Scanning electron microscopy showing a conjugate formed between a T lymphocyte and an antigen presenting cell. (pasteur.fr)
  • It is worth noting the long shape of the T cell (Tc) polarized towards the antigen presenting cell (APC) and the membrane protrusions that adhere the T lymphocyte to the antigen presenting cell. (pasteur.fr)
  • Slide for ICC-Jurkat Cell Slide (Human (14yrs, Male) T lymphocyte acute T cell leukemia) (5 slides/pk)-Alpha Diagnostic International Inc. (4adi.com)
  • Jurkat Incucyte® NucLight Red Cells are supplied as 1 mL cryopreserved vials (1.5 x 10 6 cells/mL in 90% FBS and 10% DMSO) containing a stable population of human T lymphocyte cells expressing the Incucyte® NucLight Red fluorescent protein, restricted to the nucleus. (essenbioscience.com)
  • In T cells, activation of CRAC leads to short term effects (reduced lymphocyte motility) and long term effects (altered gene expression & production of cytokines, ex. (fidelta.eu)
  • Imparting these modifications to GlyCAM-1, together with appropriate fucosylation, yields enhanced rolling ligands for both peripheral blood lymphocytes and Jurkat cells in flow chamber assays as compared with those generated with exogenous fucosyltransferase. (nih.gov)
  • Either sulfation modification results in an increased number of tethered and rolling lymphocytes, a reduction in overall rolling velocity associated with more frequent pausing of the cells, and an enhanced resistance of rolling cells to detachment by shear. (nih.gov)
  • To investigate the requirement for CD2 expression in activation of T lymphocytes via the CD3-Ti antigen/MHC receptor complex, we produced and characterized a series of CD2- Jurkat variants. (rupress.org)
  • Cui J, Bian JS, Kagan A, McDonald TV (2002) CaT1 contributes to the stores-operated calcium current in Jurkat T-lymphocytes. (springer.com)
  • G proteins are membrane-bound molecules involved in coupling of surface receptors with signal transduction effector systems in multiple cell types including T lymphocytes. (pasteur.fr)
  • Results were again significantly better than for l-PEI, although further research into the response of individual T cells to the transfection agent will be necessary, before either method can be used to routinely transfect primary T lymphocytes. (mdpi.com)
  • The Jurkat Whole Cell Lysate is derived from human. (fishersci.com)
  • Protein lysates from HIV infected Jurkat T cells and Jurkat T cells stably transfected with HIV Tat and Tat deletion mutants were subjected to quantitative slot blot analysis, western blot analysis and redox 2 dimensional (2D) gel electrophoresis to analyze the effects of SMX-HA on the thiol proteome. (biomedcentral.com)
  • The activation of procaspase-3, procaspase-7, procaspase-8, and procaspase-10 as well as cleavage of the caspase-3 substrate poly (ADP-ribose) polymerase (PARP), was evaluated with Western-blott of whole cell lysates. (arvojournals.org)
  • Cell pellets, lysates, nuclear extracts, cytoplasm / S100 extracts are available from a broad range of mammalian cell lines. (ipracell.be)
  • Developed by Sharpless and coworkers ( 8 ) and Meldal and colleagues ( 9 ), this transformation forms a triazole from an azide and a terminal alkyne, which is activated in the reaction by a Cu catalyst ( Fig. 1 A ). Click chemistry has prevailed in applications where toxicity is irrelevant, such as in probing enzyme activities in cell lysates ( 11 ) or visualizing biomolecules in fixed cells ( 12 , 13 ). (pnas.org)
  • Induction of apoptosis was measured by annexin-V fluorescein isothiocyanate and the level of active caspase 3 positive cells by intracellular staining and flowcytometry. (ijpsonline.com)
  • FasL (10ng/ml) induced a reduction in the total intracellular GSH concentration of Jurkat cells that was inhibited by the pan-caspase inhibitor Z-VAD-FMK, the caspase 8 inhibitor Z-IETD-FMK, and the caspase 9 inhibitor Z-LEHD-FMK. (aacrjournals.org)
  • Amina S, Hashii M, Ma WJ, Yokoyama S, Lopatina O, Liu HX, Islam MS, Higashida H (2010) Intracellular calcium elevation induced by extracellular application of cyclic-ADP-ribose or oxytocin is temperature-sensitive in rodent NG108-15 neuronal cells with or without exogenous expression of human oxytocin receptors. (springer.com)
  • A 60 Hz uniform electromagnetic field promotes human cell proliferation by decreasing intracellular reactive oxygen species levels. (emf-portal.org)
  • Intracellular Ca(2+) levels in rat ventricle cells exposed to extremely low frequency magnetic field. (emf-portal.org)
  • The original Th paradigm contrasted IFNγ-producing Th1 cells, important for host defense against intracellular pathogens, with Th2 cells, which produce IL-4, IL-5, IL-13 and are involved in the protection against parasitic infections. (nature.com)
  • The magnitude of the mobilization of the intracellular Ca 2+ was similar in the absence of the p56 lck activity in JCaM1.6 cells as in Jurkat cells, however, it was slightly but reproducibly delayed compared to that in the wild type cells. (elsevier.com)
  • they do not express surface CD3 or produce the T cell receptor alpha/beta heterodimer. (wikipedia.org)
  • Since they are deficient in the TCR complex, these cells are a useful tool for transfection studies using T cell receptor alpha and beta chain genes and are widely used in labs in which T cell receptor gene transfer technologies are studied. (wikipedia.org)
  • The J.gamma1 subline contains no detectable phospholipase C-gamma1 (PLC-γ1) protein and therefore has profound defects in T cell receptor (TCR) calcium mobilization and activation of nuclear factor of activated T cells (NFAT, an important transcription factor in T cells). (wikipedia.org)
  • The IS consists of a central supramolecular activation cluster (cSMAC) that is highly enriched in the T cell receptor (TCR) and its associated peptide-MHC (pMHC) and is surrounded by a peripheral ring [peripheral (p)SMAC] of the LFA-1-ICAM-1 adhesion proteins ( 1 , 2 ). (pnas.org)
  • ADCC is an immune mechanism through which Fc receptor-bearing effector cells can recognize and kill antibody (Ab)-coated target cells expressing antigens on their surface. (invivogen.com)
  • ADCC is triggered by the cross-linking between antigen-bound Abs and the Fc receptor CD16A at the surface of immune effector cells. (invivogen.com)
  • Activation was not seen when cells were attached to the substrates using antibodies directed to other cell surface proteins including CD71 (transferrin receptor), CD7, and CD11a (LFA-1), demonstrating the specificity of activation for immobilized anti-CD3 antibodies. (jimmunol.org)
  • Sustained increase in [Ca 2+ ] c (Δ[Ca 2+ ] c ) is a critical early signal from T-cell receptor (TCR/CD3). (springer.com)
  • The PTEN-deficient cells were also hyperresponsive to T-cell receptor (TCR) stimulation, as measured by Itk kinase activity, tyrosine phosphorylation of phospholipase C-γ1, and activation of Erk compared to those in PTEN-replete cells. (asm.org)
  • The CB1 receptor is mainly expressed in the central nervous system, whereas the CB2 receptor is predominantly expressed in immune cells ( 4 ). (aacrjournals.org)
  • Given that mature T cells which lack antigen receptors (CDl-Ti) are refractory to stimulation through CD2 or other accessory molecules, T cell receptor components likely play a critical role in coupling surface receptors with signal transduction effectors. (pasteur.fr)
  • It has recently been proposed that modulation of T cell receptor components with MAbs results in a physical loss or functional inactivation of G protein(s). (pasteur.fr)
  • 43- and 41-kDa G protein alpha chains are ADP ribosylated with cholera (CTX) and pertussis (PTX) toxins, respectively, in wild type and receptor minus cell populations. (pasteur.fr)
  • Importantly, AlF4- can also induce polyphosphoinositide turnover in Jurkat cells whose T cell receptor proteins have been modulated with anti-CD3 MAb. (pasteur.fr)
  • DEF6 is highly expressed in T cells and plays an immunoregulatory role in cell polarity-induced immunological synapse (IS) formation, T cell receptor (TCR) signalling, T cell activation, differentiation and inflammatory responses. (nottingham.ac.uk)
  • Human fetal retinal pigment epithelium induced apoptosis in Jurkat cells involves caspase-3 and -7 activation as well as PARP cleavage, but not activation of the death receptor-associated initiator caspases: caspase-8 and -10. (arvojournals.org)
  • Mechanistically, T cell receptor stimulation induces cyclosporine A-sensitive histone modifications and P300/CBP acetylase recruitment at these elements in activated CD4 + T cells. (nature.com)
  • Differentiation of Th subsets requires the integration of signals generated by the engagement of the T cell receptor (TCR) and by cytokines present at the time of stimulation: Th1 cells are generated in the presence of IL-12 or IFNγ, while IL-4 promotes Th2 differentiation 1 . (nature.com)
  • CD27 is a member of the tumor necrosis factor (TNF) receptor superfamily, which includes the T cell co-stimulatory receptors OX40, 4-1BB and herpesvirus entry mediator (HVEM). (bpsbioscience.com)
  • Here we show that capsaicin, the pungent ingredient of hot chili pepper, blocks receptor-stimulated Ca 2+ entry in Jurkat T cells. (elsevier.com)
  • Block of Ca 2+ entry by capsaicin is identical whether CCE is evoked by T-cell receptor (TCR) stimulation, heterologous muscarinic M1 receptor stimulation, or via thapsigargin depletion of internal Ca 2+ stores. (elsevier.com)
  • Block of Ca2+ entry by capsaicin is identical whether CCE is evoked by T-cell receptor (TCR) stimulation, heterologous muscarinic M1 receptor stimulation, or via thapsigargin depletion of internal Ca2+ stores. (elsevier.com)
  • The interleukin (IL) 13 receptor α2 (IL13Rα2) is a glioma-restricted cell-surface epitope not otherwise detected within the central nervous system. (aacrjournals.org)
  • Here, we describe a novel approach for targeting glioblastoma multiforme (GBM) with IL13Rα2-specific cytolytic T cells (CTLs) by their genetic modification to express a membrane-tethered IL13 cytokine chimeric T-cell antigen receptor, or zetakine. (aacrjournals.org)
  • Effect of Embelin on the activity of the caspase pathway and effect of the caspase inhibitors, z-DEVD-fmk and Ac-LEHD-CHO, on Embelin-treated Jurkat cells. (nih.gov)
  • This finding suggests that the ERK pathway plays an important role in the maintenance and promotion of survival of T cells under the condition of stimulation by Fas. (aspetjournals.org)
  • In the present study, we tested the hypothesis that rapid activation of the ERK pathway after PMA treatment protects Jurkat cells from MG-induced apoptosis. (aspetjournals.org)
  • In contrast, it was shown that cannabinoids were cytotoxic in leukemic cells and that they inhibited neuronal progenitor cell differentiation through attenuation of the ERK pathway ( 15 ). (aacrjournals.org)
  • In vertebrates, convergence and extension (CE) cell movements are regulated by the non-canonical Wnt/Planar cell polarity (PCP) signalling pathway which requires the activation of its downstream effectors, Rho GTPases. (nottingham.ac.uk)
  • Although it has been shown that the zebrafish orthologous, Def6a and Swap70b, act downstream of Wnt5b or Wnt11 signalling pathway, respectively, regulating the CE cell movements during gastrulation, little is known about the underlying molecular mechanisms and direct downstream targets of Def6a and Swap70b. (nottingham.ac.uk)
  • HFRPE cells inhibited the proliferation of Jkt cells by inducing apoptosis through a FasL-independent pathway. (umn.edu)
  • This research sought to determine if TAL-1 influences expression of proteins involved in the NF-kB signaling pathway and thus, resistance to cell death. (bsu.edu)
  • One of the signaling pathways reported to be targeted by vitamin D is the NF-κB pathway, which is highly active in most immune cell types, including T cells. (elsevier.com)
  • However, the effects of vitamin D on the NF-κB pathway in T cells are not fully understood. (elsevier.com)
  • Therefore, we conclude that vitamin D does not modulate the activity of the NF-κB pathway in Jurkat cells. (elsevier.com)
  • Children with T-lineage leukaemia are more resistant to a variety of drugs in vitro than B-cell and acute myeloid leukaemia [ 4 ]. (ijpsonline.com)
  • HFRPE cells induced a stronger inhibitory effect on Jkt cells at higher in vitro passages. (umn.edu)
  • Thus, although these biomolecules have been studied in vitro and in static systems, their dynamic behavior in living cells is not well defined. (pnas.org)
  • A single cycle of treatment with temozolomide, alone or combined with O(6)-benzylguanine, induces strong chemoresistance in melanoma cell clones in vitro: role of O(6)-methylguanine-DNA methyltransferase and the mismatch repair system. (openrepository.com)
  • Thus, alkylamides present in E. purpurea suppress the ability of activated Jurkat T cells to produce IL-2 independently of direct, cytotoxic effects. (bastyr.edu)
  • Ultimately, the effector cells release cytotoxic granules which kill the target cells [3]. (invivogen.com)
  • In order to check if fibronectin allowed the real-time monitoring of the cytotoxic action of several compounds, we incubated Jurkat and L1236 cells with different drugs, such as FasL (50 ng/mL), oxaliplatin (100 μM), bendamustine (200 μM), or trabectedin (10 nM). (nih.gov)
  • As seen in Figure 4A, the cell growth and the cytotoxic response were only monitored in the presence of fibronectin. (nih.gov)
  • Lymphoblastic leukemia cells (Jurkat cells) were used to evaluate the cytotoxic effects of n-hexane extract of C. intybus . (academicjournals.org)
  • Flunarizine is a Ca2+ channel blocker that can be either cytoprotective or cytotoxic, depending on the cell type that is being examined. (semanticscholar.org)
  • We show here that flunarizine was cytotoxic for Jurkat T-leukemia cells, as well as for other hematological maligancies, but not for breast or colon carcinoma cells. (semanticscholar.org)
  • The results showed that ZER is selectively cytotoxic to Jurkat cells in a dose and time-dependent manner with IC50 of 11.9 ± 0.2, 8.6 ± 0.5 and 5.4 ± 0.4 μg/mL at 24, 48 and 72 hours of treatment, respectively. (upm.edu.my)
  • ZER is not as cytotoxic as doxorubicin, which imposed an inhibitory effect on Jurkat cells with IC50 of 2.1 ± 0.2, 1.8 ± 0.15, 1.5 ± 0.07 μg/mL after 24, 48 and 72 hours treatment, respectively. (upm.edu.my)
  • Freies Calcium im Zytosol wurde während der Exposition bei einem extrem niederfrequenten Magnetfeld in Jurkat E6.1- Zellen gemessen, die bei verschiedenen Phasen des Zellzyklus synchronisiert waren (G 0 /G 1 , S und G 2 -M-Phasen). (emf-portal.org)
  • The study of the epigenetic modifications of the Ifng locus in Th1 cells and Il4 locus in Th2 cells showed that these genes are associated with permissive histone marks in the relevant lineage, while they are enriched with repressive modifications in the lineages that do not express the cytokine 8 . (nature.com)
  • Our findings provide biological evidence that EGCG induces Th1/Th2 cytokine mRNA expression via H 2 O 2 production followed by activation of ERK or JNK in Jurkat T cells. (elsevier.com)
  • Our findings provide biological evidence that EGCG induces Th1/Th2 cytokine mRNA expression via H2O2 production followed by activation of ERK or JNK in Jurkat T cells. (elsevier.com)
  • Human IL13-zetakine + CD8 + CTL transfectants display IL13Rα2-specific antitumor effector function including tumor cell cytolysis, T C 1 cytokine production, and zetakine-regulated autocrine proliferation. (aacrjournals.org)
  • Our IL13-zetakine is a prototype of a new class of chimeric immunoreceptors that signal through an engineered immune synapse composed of membrane-tethered cytokine muteins bound to cell-surface cytokine receptors on tumors. (aacrjournals.org)
  • The ImageXpress® Pico system does more than imaging-it offers unparalleled analysis capabilities that simplifies image analysis for cell-based assays. (moleculardevices.com)
  • These small, round cells grow readily in suspension and have also been used as a model system for various cell viability and apoptosis assays as demonstrated here. (moleculardevices.com)
  • Functionally tested in ADCC assays with various target cells from our expanding collection of Raji-derived target cells (e.g. (invivogen.com)
  • Data in this poster is presented for the transient transfection of a variety of difficult-to-transfect cells and their use in downstream assays. (selectscience.net)
  • Specifically, the transfection of Jurkat,CHO, human skeletal muscle cells and primary neuronal cells using MaxCyte's scalable electroporation technology and their performance in a variety of cell-based assays is described. (selectscience.net)
  • These cells are fully validated for use with the IncuCyte® live-cell imaging and analysis system and are ideal for use in real-time cell counting studies, co-cultures and chemotaxis assays. (essenbioscience.com)
  • Automated imaging tools can provide valuable information for improving routine cell culturing techniques and increasing the effectiveness and reproducibility of downstream cell-based assays. (biotekinstruments.ru)
  • Agilent cell metabolism assays detect discrete changes in cell bioenergetics in real time, providing a window into the critical functions that provide ATP, the energy that cells need for activation, signaling, proliferation, and biosynthesis. (biotekinstruments.ru)
  • Effect of Embelin on the proliferation of Jurkat cells. (nih.gov)
  • Lower concentrations from 6.25 to 25 microg/mL significantly decreased IL-2 production but not cell viability. (bastyr.edu)
  • Two Echinacea-derived alkylamides significantly depressed IL-2 production but not cell viability in a dose-dependent manner. (bastyr.edu)
  • All compounds used in this experiment caused a dramatic reduction in cell viability within 24 hours. (moleculardevices.com)
  • Decline of cell viability to 50 % was observed at 67.78±3.88, 60.97±3.36 and 52.11±2.50 µM concentration after 24, 48 and 72 h incubation with pterostilbene, respectively. (ijpsonline.com)
  • xCELLigence system for real-time label-free monitoring of growth and viability of cell lines from hematological malignancies. (nih.gov)
  • With a fibronectin substrate, nonadherent cells deposited in a monolayer configuration, and consequently, the cell growth and viability were robustly monitored. (nih.gov)
  • Disclaimer: We cannot guarantee cell viability if the cells are not thawed immediately upon receipt and grown according to handling procedure. (creative-biolabs.com)
  • The results of cell viability showed that both the dexamethazone and NaNO 2 significantly increased the percentage of dead cells. (exp-oncology.com.ua)
  • Cell health and viability measurements provide essential insight into a broad range of biological processes and treatment responses. (biotekinstruments.ru)
  • I have transfected Jurkat cells with a plasmid contating my gene and resistance to G418. (protocol-online.org)
  • These cells stably express the Lucia luciferase reporter gene under the control of an ISG54 minimal promoter fused to six NFAT response elements. (invivogen.com)
  • These findings suggest that PWGPE-induced apoptosis in Jurkat human leukemia cells, is mediated by mitochondrial depolarization and caspase-3 cleavage, and depends on ROS generation and regulation of epigenetic gene silencing. (rsc.org)
  • When Bcl-2 gene was overexpressed, Jurkat cells were resistant to the drug. (aspetjournals.org)
  • The abundance of 3-methylcrotonyl-CoA carboxylase mRNA was lower in cells in medium D than cells in media N and S. The enrichment of biotinylated histones was higher at the SMVT promoter 1 in HepG2 and Jurkat cells in medium S compared with the corresponding cells in media D and N, presumably repressing the SMVT gene. (biomedsearch.com)
  • These findings provide the first evidence that a cell type-specific factor such as SATB1 binds to the base of chromatin loops in vivo and suggests that a specific chromatin loop domain structure is involved in T cell-specific gene regulation. (ovid.com)
  • The aims of this study were to investigate the effects of P. gingivalis on PAR-2 gene expression in human GF and Jurkat T cells, using quantitative real-time PCR, and to evaluate the involvement of gingipains. (uzh.ch)
  • Effect of extremely low frequency magnetic fields on cell proliferation and gene expression. (emf-portal.org)
  • Differentiation is maintained by a network of subset-specific transcription factors and is stabilized through multiple cell divisions by epigenetic processes that regulate accessibility of regulatory chromatin regions, and promote heritable gene expression patterns. (nature.com)
  • Post-translational modifications of histone tails are key in influencing gene expression 7 , and were shown to be important for T helper cell differentiation. (nature.com)
  • The involvement of the antiapoptotic human gene bcl-x(L) was determined by using Jkt cells that were stably transfected with bcl-x(L). The role of cell-cell contact in the induction of apoptosis was evaluated in a transwell system in the presence or absence of neutralizing antibodies against IFN-γ and tumor necrosis factor (TNF)-α. (umn.edu)
  • The bcl-x(L) gene may play a role in preventing HFRPE cell-induced apoptosis in Jkt cells. (umn.edu)
  • Alternative splicing is a key mechanism for generating protein diversity and regulating gene expression and, therefore, plays an important role in cell function and development. (frontiersin.org)
  • Hour-long calcium elevations are necessary for efficient gene expression during T cell activation and proliferation. (wright.edu)
  • We found that 1α,25(OH) 2 D 3 does not inhibit the induction of IκBα degradation and the expression of an NF-κB-dependent reporter gene in Jurkat cells following treatment with PMA/ionomycin. (elsevier.com)
  • Furthermore, we demonstrate that 1α,25(OH) 2 D 3 does not block the induction of CD69, which is an NF-κB target gene and an early T cell activation marker. (elsevier.com)
  • This study was to examine the link between astrocyte elevated gene-1 (AEG-1) and hypoxia induced-chemoresistance in T-cell non-Hodgkin's lymphoma (T-NHL), as well as the underlying molecular mechanisms. (springer.com)
  • Extremely Low Frequency Magnetic Fields Do Not Elicit Oxidative Stress in MCF10A Cells. (emf-portal.org)
  • Mechanisms of cell death in oxidative stress. (semanticscholar.org)
  • Taken together, these results suggest that PMA-induced ERK activation can protect Jurkat cells from methylglyoxal-induced apoptosis and that activated ERK exerts its antiapoptotic effects on mitochondria by inhibiting activated JNK-induced permeabilization of the outer mitochondrial membrane. (aspetjournals.org)
  • jurkat , stimulation , pma and 1 more. (protocol-online.org)
  • Similarly, little is known about the relationship between F-actin and changes in cell shape or other parameters of activation, such as the appearance of proteins newly phosphorylated on tyrosine, that occur after stimulation via the CD3/Ti complex. (jimmunol.org)
  • Stimulation of CD28 triggers an association between CD28 and phosphatidylinositol 3-kinase in Jurkat T cells. (rupress.org)
  • Here we demonstrate that stimulation of CD28, either by B7, its natural ligand, or by the anti-CD28 monoclonal antibody 9.3, induces an association between CD28 and phosphatidylinositol 3-kinase (PI3-K) in Jurkat T cells, raising the possibility that an interaction with PI3-K contributes to CD28-mediated signaling. (rupress.org)
  • Moreover, we show that HCV-core-transduced Jurkat cells are able to suppress CD4 + and CD8 + T-cell responses to anti-CD3 plus anti-CD28 stimulation. (portlandpress.com)
  • These data support the idea that PH domain-mediated association with the plasma membrane is required for Itk activation, provide evidence for a negative regulatory role of PTEN in TCR stimulation, and suggest that signaling models based on results from Jurkat T-cell lines may underestimate the role of PI3K in TCR signaling. (asm.org)
  • Upon application of capsaicin to Jurkat cells in culture we observed an inhibition of interleukin-2 (IL-2) production in response to TCR stimulation. (elsevier.com)
  • In comparison, stimulation of the Jurkat cells with PMA resulted in rapid induction of c-jun and c-fos within 1 h. (allenpress.com)
  • Purpose: Activation of T cells by direct stimulation with phorbol 12-myristate 13-acetate (PMA) and ionomycin (Io) results in numerous downstream signals that activate pathways enabling T cells to proliferate and produce cytokines. (ac.rs)
  • Some, but not all, of a series of novel pyrrolo-1,5-benzoxazepines (PBOXs) induce apoptosis as shown by cell shrinkage, chromatin condensation, and DNA fragmentation in three human cell lines, HL-60 promyelocytic, Jurkat T lymphoma, and Hut-78 s.c. lymphoma cells. (aspetjournals.org)
  • We pretreated Jurkat cells for 1 h with glucosamine (GlcN), PUGNAc (O-(2-acetamido-2-deoxy-d-glucopyranosylidene)-amino-N-phenylcarbamate) an inhibitor of O-GlcNAcase, or a high level of glucose to induce elevated levels of O-GlcNAc. (eur.nl)
  • Heat inactivation or chemical inhibition of cysteine proteases abolished the capacity of P. gingivalis to induce PAR-2 expression in Jurkat T cells. (uzh.ch)
  • In conclusion, P. gingivalis can induce PAR-2 expression in GF and Jurkat T cells, potentially attributed to its gingipains. (uzh.ch)
  • Plant-derived cannabinoids, including Δ9-tetrahydrocannabinol (THC), induce apoptosis in leukemic cells, although the precise mechanism remains unclear. (aacrjournals.org)
  • More recently, we reported that the immunosuppressive property of THC can be attributed, at least in part, to its ability to induce apoptosis in T cells and dendritic cells through ligation of CB2 receptors and that the latter was regulated by activation of nuclear factor-κB ( 8 ), recruiting both intrinsic and extrinsic pathways of apoptosis. (aacrjournals.org)
  • Interestingly, we also found that THC and other cannabinoids could induce apoptosis in transformed murine and human T cells ( 9 ), including primary acute lymphoblastic human leukemia cells, and furthermore that the treatment of mice bearing a T-cell leukemia with THC could cure ∼25% of the mice ( 10 ). (aacrjournals.org)
  • These findings are consistent with studies showing that THC and other cannabinoids can induce apoptosis in a variety of tumor cell lines, thereby raising the possibility of the use of cannabinoids as novel anticancer agents ( 11 ). (aacrjournals.org)
  • The precise mechanism through which cannabinoids induce apoptosis is under active investigation and may vary based on cell type. (aacrjournals.org)
  • In normal and transfected neural cells, vascular endothelial cells, and Chinese hamster ovary cells, cannabinoid treatment was shown to induce activation of ERK ( 12 ), c-Jun NH 2 -terminal kinase (JNK), and p38 ( 13 , 14 ). (aacrjournals.org)
  • In glioma cells, THC was shown to induce apoptosis via ceramide generation ( 16 ). (aacrjournals.org)
  • It has previously been shown that Embelin inhibits proliferation, promotes apoptosis, and increases sensitivity and reduces resistance to chemotherapy drugs, in various types of tumor cells. (nih.gov)
  • Midazolam induces cellular apoptosis in human cancer cells and inhibits tumor growth in xenograft mice. (semanticscholar.org)
  • 18β-Glycyrrhetinic acid potently inhibits Kv1.3 potassium channels and T cell activation in human Jurkat T cells. (semanticscholar.org)
  • 2002) Bay 11-7082 inhibits transcription factor NF-kB and induces apoptosis of HTLV-1- infected T cell lines and primary adult T-cell leukemia cells. (bpsbioscience.com)
  • In the present study, we demonstrate that pro-caspase-3 is present in the mitochondrial fraction of Jurkat T cells in a complex with the chaperone proteins Hsp60 and Hsp10. (uniprot.org)
  • This finding suggests that the release of mitochondrial Hsps may also accelerate caspase activation in the cytoplasm of intact cells. (uniprot.org)
  • The protection of cells against MG-induced apoptosis by PMA involves inhibition of mitochondrial depolarization and of caspase cleavage. (aspetjournals.org)
  • These data are in agreement with our previous published report indicating HFRPE cell-induced loss of mitochondrial membrane potential in Jkt cells and the absence of FasL and TRAIL involvement in HFRPE cell-mediated apoptosis. (arvojournals.org)
  • Albumin prevents mitochondrial depolarization and apoptosis elicited by endoplasmic reticulum calcium depletion of neuroblastoma cells. (semanticscholar.org)
  • Thanks to their rounded morphology and uniform size, Jurkat cells are fairly easy to image and count. (moleculardevices.com)
  • Jurkat, clone E6-1 (Human Acute T Cell Leukemia) whole blood lymph cell lysate 2mg/mL supplied in SDS sample buffer containing 5% Beta-mercaptoethanol. (fishersci.com)
  • Jurkat (Human Acute T cell Leukemia) cell membrane protein lysate was prepared by isolating the membrane protein from whole tissue homogenates using a proprietary technique. (genetex.com)
  • In addition to constitutive membrane association of Itk, unstimulated JTAg T cells also exhibited constitutive phosphorylation of Akt on Ser-473, an indication of elevated basal levels of the phosphatidylinositol 3-kinase (PI3K) products PI-3,4-P 2 and PI-3,4,5-P 3 in the plasma membrane. (asm.org)
  • However, no significant association of Bad translocation with phosphatidylinositol 3-kinase/Akt and protein kinase A signaling pathways was noted when treated cells were examined in relation to phosphorylation status of Bad by Western blot and localization of Bad to mitochondria by confocal analysis. (aacrjournals.org)
  • Jurkat cells were treated with various concentrations of Embelin and the effects of Embelin on the inhibition of growth of Jurkat cells were evaluated. (nih.gov)
  • Therefore, inhibition of CRAC (Icrac) is expected to modulate T cell activation and channels are potential target for new anti-inflammatory drugs as RA and psoriasis. (fidelta.eu)
  • 1. Borner C and Kraus J (2013) Inhibition of NF-kB by Opioids in T cells. (bpsbioscience.com)
  • These combined results suggest that the HFRPE-mediated suppression of primary T cells may also be mediated by the induction of apoptosis. (umn.edu)
  • Synergistic induction of apoptosis was also observed in other human leukemia cell types (eg, Jurkat, NB4). (bloodjournal.org)
  • I want to perform limiting dilution in order to obtain a cell population from a single clone. (protocol-online.org)
  • Detailed protocols for single-cell clone generation, screening, and correct targeting verification are provided. (jove.com)
  • The environment around cells in humans and other animals is different to that found around laboratory-grown cells, and so more research will be needed to check whether this difference affects which method of infection the virus uses. (elifesciences.org)
  • By cDNA transfection Jurkat cell lines were generated that stably express either IL-8R1 or IL-8R2 (J-IL8R1 and J-IL8R2). (nih.gov)
  • High Throughput Transfection of Stem Cells, Primary Cells and Difficult-to-Transfect Cell Lines: Jurkat, CHO, Human Skeletal Muscle Cells & Primary Neuronal Cell Transfection using a Scalable, Electroporation-Based Technology. (selectscience.net)
  • This demonstrates how large-scale electroporation can be used to eliminate the reliance on stable cell lines and costly transfection reagents, by producing large numbers of quality transfected cells for use in high throughput cellular screening and profiling. (selectscience.net)
  • Linear polyethylenimine (l-PEI), one of the most popular non-viral transfection agents for mammalian cells in general, only achieves transfection rates in the single digit percentage range for these cells. (mdpi.com)
  • Baba Y, Kurosaki T (2009) Physiological function and molecular basis of STIM1-mediated calcium entry in immune cells. (springer.com)
  • Real-time measurement of cytosolic free calcium concentration in Jurkat cells during ELF magnetic field exposure and evaluation of the role of cell cycle. (emf-portal.org)
  • Echtzeit-Messung der freien zytosolischen Calcium-Konzentration in Jurkat-Zellen während ELF-Magnetfeld-Exposition und Bewertung der Zellzyklus-Rolle]. (emf-portal.org)
  • Calcium signalling in human neutrophil cell lines is not affected by low-frequency electromagnetic fields. (emf-portal.org)
  • Finally, we showed that in agreement with a previous study, PIP3 specifically, can elicit calcium elevations in Jurkat T cells. (wright.edu)
  • Jurkat cells naturally express a functional NFAT (nuclear factor of activated T cells) transcription factor, which is involved in the early signaling events in antibody-dependent cellular cytotoxicity (ADCC) [1, 2]. (invivogen.com)
  • Jurkat-Lucia™ NFAT-CD16 effector cells were then co-incubated with targets cells for 6 hours. (invivogen.com)
  • Increased ADCC activity mediated by IgG1 compared to IgG1fut (non-fucosylated): Raji-hCTLA4, Raji-hPD-1, and Raji-hPD-L1 cells were incubated with Jurkat-Lucia™ NFAT-CD16 effector cells and corresponding IgG1 or IgG1fut specific mAbs. (invivogen.com)
  • Jurkat-Lucia™ NFAT-CD16 cells are guaranteed mycoplasma-free. (invivogen.com)
  • In these experiments, Jurkat cells were incubated with antibody-coated substrates for 1 to 30 min at 37 degrees C and actin rearrangements were visualized on fixed, detergent-permeabilized cells using rhodamine-conjugated phalloidin. (jimmunol.org)
  • The isolated Jurkat (Human Acute T cell Leukemia) cell membrane protein is then Western analyzed by either GAPDH or beta-actin antibody to confirm there is no signal or very weak signal. (genetex.com)
  • In view of the importance of the T cell activation process, we herein examined G proteins in untreated or antibody-modulated Jurkat T cells as well as in genetic variants lacking either CD3-Ti or CD2 surface receptors. (pasteur.fr)
  • Comparison of ADCC potency for native and engineered anti-human CD20 isotypes: Raji-Null cells were incubated with gradient concentrations of Anti-hCD20 or Anti-β-galactosidase (β-gal) mAbs for 1 hour. (invivogen.com)
  • Jurkat cells were incubated with different concentrations of pterostilbene alone or in combination with L-asparaginase for 24, 48 and 72 h. (ijpsonline.com)
  • After 6 h of challenge with ascending concentrations of P. gingivalis, PAR-2 expression was up-regulated in both cell types by approximately 5-fold, compared with the control. (uzh.ch)
  • We are a global provider of human and animal biospecimens: including frozen & FFPE tissue, DNA, RNA, total proteins, blood products and primary cells. (amsbio.com)
  • The aim of the present study was to investigate the anticancer effect of a purified white grape pomace extract (PWGPE) in acute lymphoblastic leukemia Jurkat cells and to characterize the underlying mechanism. (rsc.org)
  • TAL-1, ectopically expressed in 60% of T-cell acute lymphoblastic leukemia (T-ALL) patients, may contribute to poor chemotherapy response. (bsu.edu)
  • T cells interacting with antigen-presenting cells (APCs) form an "immunological synapse" (IS), a bull's-eye pattern composed of a central supramolecular activation cluster enriched with T cell receptors (TCRs) surrounded by a ring of adhesion molecules (a peripheral supramolecular activation cluster). (pnas.org)
  • The activation of T cells during an immune response begins with contact and the formation of a stable junction between the T cell and an APC. (pnas.org)
  • Because of its high concentration of the TCR and other signaling molecules, it was originally thought the IS might be needed for sustained signaling, which is required for T cell activation ( 2 ). (pnas.org)
  • Identification of a putative regulator of early T cell activation genes. (invivogen.com)
  • T cell activation via CD3/Ti linked pathways results in the polymerization and reorganization of actin. (jimmunol.org)
  • Analysis of cell shape and F-actin morphology during the first 30 min of activation revealed a unique pattern that was observed only when cells were stimulated with anti-CD3 antibodies. (jimmunol.org)
  • The T cell surface molecule CD28 can provide costimulatory signals that permit the full activation of T cells. (rupress.org)
  • Apoptosis is characterized by cell shrinkage along with the activation of biochemical pathways including caspases and endonucleases. (aacrjournals.org)
  • Potassium loss associated with cell volume decrease has been shown to be an initial regulator in the activation of these enzymes and in the further progression of the cell death program, but the mechanisms involved in the modulation and activation of K + efflux have not been clearly defined. (aacrjournals.org)
  • The importance of PH domain-mediated interactions with the plasma membrane is well illustrated by the mechanisms of activation of the ubiquitous serine/threonine kinase Akt (also known as protein kinase B) and the B-cell and mast cell protein tyrosine kinase (PTK) Btk. (asm.org)
  • We showed previously that Jurkat cells treated with MG rapidly undergo apoptosis via c-Jun N-terminal kinase (JNK) activation. (aspetjournals.org)
  • Extremely low frequency electromagnetic field sensitizes cisplatin-resistant human ovarian adenocarcinoma cells via P53 activation. (emf-portal.org)
  • DEF6 and SWAP70 are guanine nucleotide exchange factors (GEFs) catalysing the activation of Rho GTPases to regulate the re-arrangement of actin cytoskeleton, cell polarity and cell movements. (nottingham.ac.uk)
  • Passaging and IFN-γ activation strengthened the inhibitory effect of HFRPE cells on the proliferation of Jkt cells. (umn.edu)
  • Thus, capsaicin and its numerous analogs may have potential use as immunomodulatory drugs and should be further investigated in models of inflammation and T-cell activation. (elsevier.com)
  • Previous findings strongly suggest that T cell activation is accompanied by cytosolic alkalinization. (wright.edu)
  • Here, we show that pH changes in Jurkat T cells following activation with mitogenic lectin, phytohemagglutinin (PHA), depends on the length of time of exposure and the concentration (potency) of the mitogen. (wright.edu)
  • For full understanding of ion fluxes involved in this process, it is important to distinguish CRAC channel subtype functions in these cells during activation as well as elucidate the pH mediated changes in Ca2+. (wright.edu)
  • In Discussion, published data contradicting the described findings on down regulation of Notch signaling upon T cell activation should be discussed [Sierra RA, Thevenot P, Raber PL et al. (sciencematters.io)
  • Inducible T cell activation is regulated predominantly at the transcriptional level. (ac.rs)
  • Neither c-kit nor the antioxidative system were activated, excluding their role in Jurkat T-cell activation in the absence of exogenous c-kit ligand SCF. (ac.rs)
  • While many immunologically relevant genes undergo alternative splicing, the role of regulated splicing in T cell immune responses is largely unexplored, and the signaling pathways and splicing factors that regulate alternative splicing in T cells are poorly defined. (frontiersin.org)
  • We show that SC35 colocalizes with RNA polymerase II in activated T cells and spatially overlaps with H3K27ac and H3K4me3, which mark transcriptionally active genes. (frontiersin.org)
  • Several T cell genes, such as CD44 and CD45 , undergo alternative splicing to produce distinct protein isoforms ( 7 , 8 ). (frontiersin.org)
  • Here, we show using a combination of Jurkat T cells, human primary T cells, and ex vivo naïve and effector virus-specific T cells isolated after influenza A virus infection that SC35 phosphorylation is induced in response to stimulatory signals. (frontiersin.org)
  • Here, we show that a prototype zetakine immunoreceptor with a membrane-tethered IL13 E13Y mutein can redirect the antigen specificity and antitumor effector mechanisms of cytolytic T cells (CTLs) to IL13Rα2 + gliomas. (aacrjournals.org)
  • RT-PCR and microarray analysis of Jurkat cells showed the expression of the GSH-efflux pumps multidrug resistance protein 1 (MRP-1) and the organic anion polypeptide transporters SLCO1B1, SLCO3A1 and SLCO4A1. (aacrjournals.org)
  • In the present work we aim to study the effect of HCV-core protein in the development of T-cells with regulatory-like function. (portlandpress.com)
  • In conclusion, we demonstrate for the first time that protein O-GlcNAc could modulate cell volume regulation. (eur.nl)
  • For quality control purposes, the isolated Jurkat (Human Acute T cell Leukemia) cell membrane protein pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. (genetex.com)
  • In the current study, we investigated the effect of THC on the upstream and downstream events that modulate the extracellular signal-regulated kinase (ERK) module of mitogen-activated protein kinase pathways primarily in human Jurkat leukemia T cells. (aacrjournals.org)
  • Placental protein 14 (PP14) is a glycoprotein produced by cells of the reproductive and hematopoietic systems that possesses antiinflammatory activity. (elsevier.com)
  • Conversely, incubation with SMX-HA led to a 2- to 3-fold increase in thiol protein oxidation as well as a significant reduction in the level of peroxiredoxin in all the cell lines, particularly in the Tat-expressing cell lines. (biomedcentral.com)
  • Hence, a novel method, BioID, which enables the promiscuous biotinylation of proximal and interacting proteins of a target protein in mammalian cells, was adapted to identify DEF6 interactome in Jurkat T cells. (nottingham.ac.uk)
  • Co-localisation of DEF6 with Coronin1A (CORO1A), an actin cytoskeleton regulator during IS formation, in resting and activated cells provided proof of principle for the interactome analysis suggesting that DEF6 is a multifunctional protein involved in the regulation of cytoskeletal organisation, transcription, mRNA splicing, protein folding/processing and metabolic processes. (nottingham.ac.uk)
  • Interactions between the protein kinase C (PKC) and Chk1 inhibitor UCN-01 and the heat shock protein 90 (Hsp90) antagonist 17-AAG have been examined in human leukemia cells in relation to effects on signal transduction pathways and apoptosis. (bloodjournal.org)
  • 13 Although the mechanism by which heat shock protein antagonists such as 17-AAG kills cells is uncertain, and may vary with the cell type, recent evidence suggests that down-regulation of Akt may play an important role in this process. (bloodjournal.org)
  • Effect of Embelin on the rate of apoptosis rate of Jurkat cells. (nih.gov)
  • abstract = "Lysophospholipids, particularly lysophosphatidylcholine (lyso-PC), have been implicated in modulating T cell functions at the sites of inflammation and atherosclerosis. (elsevier.com)
  • Based on more than 20 years of expertise gained with 4CBiotech and CILBiotech, Ipracell is offering a broad range of biologicals based on mammalian cells. (ipracell.be)
  • Glucose is a principal carbon source for ATP production and biosynthesis in the multiplication of mammalian cells by either tricarboxylic acid (TCA) or glycolytic cycle pathways. (news-medical.net)
  • Bypassing the complexities of peripheral blood mononuclear cell populations, we further showed that the recombinant PP14 could suppress IL-2 production by human Jurkat T cells stimulated with PMA plus anti-CD3 mAb. (elsevier.com)
  • ZER did not produce an adverse effect on normal human peripheral blood mononuclear cells (PBMC). (upm.edu.my)
  • It activates NF-κB and MAPK/JNK signaling upon interaction with its TNF-like ligand, CD70, which is expressed by numerous tumor cells. (bpsbioscience.com)
  • Coexposure of human leukemia cells to 17-AAG and the PKC inhibitor bisindolylmaleimide (GFX) did not result in enhanced lethality, arguing against the possibility that the PKC inhibitory actions of UCN-01 are responsible for synergistic interactions. (bloodjournal.org)
  • 18 By interfering with Cdc25C degradation, UCN-01 opposes inhibitory phosphorylation of p34 cdc2 on Thr14 and Tyr15 sites, and in so doing, abrogates checkpoint control in cells exposed to various DNA-damaging agents. (bloodjournal.org)
  • The present study examined the effects of Embelin on the proliferation of human acute T cell lymphoma Jurkat cells. (nih.gov)
  • The results showed that Embelin significantly inhibited the growth of human acute T cell lymphoma Jurkat cells. (nih.gov)
  • Pre-coating of the culture plates with fibronectin, compared to laminin, collagen, or gelatin, significantly induced the adhesion of most of the leukemia/lymphoma cells assayed (Jurkat, L1236, KMH2, and K562). (nih.gov)
  • 2007 ). T-cell lymphoma (T-NHL) accounts for approximately 15% of NHL in the United States (Tian et al. (springer.com)
  • I mean things like: G418 concentration, cell concentration or any other special conditions. (protocol-online.org)
  • Pterostilbene at a concentration of 30, 50 and 70 µM in combination with 0.5 and 0.7 IU/ml L-asparaginase reduced relative cell growth to a significant level. (ijpsonline.com)
  • The level of caspase 3 positive cells was significantly higher than control at 80 µM concentration of pterostilbene. (ijpsonline.com)
  • PWGPE induced apoptosis in Jurkat cells in a time- and concentration-dependent manner. (rsc.org)
  • The hypotonia induced cell-swelling was augmented in both GlcN and PUGNAc-treated cells and, to a lesser extent, in high glucose concentration-treated cells. (eur.nl)
  • The P. gingivalis concentration required for maximal PAR-2 induction was 4-fold greater in GF than Jurkat T cells. (uzh.ch)
  • A) The glucose uptake in Jurkat Cells is measured by using different concentration of GluTracker™: 0(Purple filled), 12.5 µM (Green), and 100 µM (Red). (news-medical.net)
  • B) After treatment, cells were washed and incubated with 50 µM AGTracker reagent and the same concentration of phloretin for another 30 minutes, according to protocol. (news-medical.net)
  • Neutrophil leukocytes, the target cells for interleukin-8 and related CXC chemokines, bear high numbers of two types of IL-8 receptors (IL-8R1 and IL-8R2). (nih.gov)
  • Jurkat cells are used to study acute T cell leukemia, T cell signaling, and the expression of various chemokine receptors. (creative-biolabs.com)
  • Thus, a variety of cell-surface receptors that have a direct bearing on tumorgenicity constitute potential targets for therapeutic intervention. (aacrjournals.org)
  • The I 9.2 and I 2.1 cell lines. (wikipedia.org)
  • Find tissues and cell lines supported by DNA array analysis to express HNRPD . (avivasysbio.com)
  • It has been known for various pharmacological activities and anticancer effects against various cell lines including breast cancer MCF-7, prostate cancer LNCaP, amelanotic melanoma C32 and renal adenocarcinoma ACHN. (academicjournals.org)
  • Identity testing is required for newly established cell lines. (creative-biolabs.com)
  • A broad range of viruses is susceptible to affecting human cell lines. (creative-biolabs.com)
  • Affordable & consistent controls from 50 tumor cell lines, ideal for IHC, in situ hybridization & NGS applications. (amsbio.com)
  • In the current study, Jurkat T cell lines expressing Tat and its deletion mutants were used to determine the effect of Tat on the thiol proteome in the presence and absence of SMX-HA revealing drug-dependent changes in the disulfide proteome in HIV infected cells. (biomedcentral.com)
  • Please note that cell lines are not for sale and unavailable for purchase from Covance. (covance.com)
  • Although the generation of single-cell clones and verification of correct reporter sequence integration is time-consuming, the resulting clonal lines are powerful tools to functionally analyze proviral integration site choice. (jove.com)
  • Broadly used cell culture models for HIV latency like JLat cell lines do not reflect clinically relevant recurrent integration sites 9 . (jove.com)
  • The workflow described herein can be used to generate T cell-derived reporter cell lines that model HIV infection, carrying a genomically integrated proviral reporter at a chosen integration site. (jove.com)
  • Apoptosis was induced by dexamethazone (1 m g/ml) or NaNO2 (7 m g/ml) added in the presence or absence of NO-synthase inhibitor N w -nitro-L-arginine methyl ester (L-NAME) (27 m g/ml) during cell culturing. (exp-oncology.com.ua)
  • 16 UCN-01 is also a cyclin-dependent kinase (CDK) inhibitor that arrests cells in G 1 . (bloodjournal.org)
  • Jurkat cells can produce interleukin 2, and are used in research involving the susceptibility of cancers to drugs and radiation. (wikipedia.org)
  • The expected increased expression of interleukin (IL)-2 mRNA, together with moderate Ki-67 upregulation, indicate the proliferation of PMA/Io treated Jurkat cells. (ac.rs)
  • Previously we found that the T-cell-specific PH domain-containing kinase Itk is constitutively membrane associated in Jurkat T cells. (asm.org)
  • Jurkat cells expressing a constitutively active MEK construct were found to be resistant to THC-mediated apoptosis and failed to exhibit decreased phospho-Bad on Ser 112 as well as Bad translocation to mitochondria. (aacrjournals.org)
  • The antiproliferative effect of MTX was significantly enhanced by the knockdown of DHFR expression by siRNA in Jurkat cells. (nih.gov)
  • Sulfation on C-6 of GlcNAc or Gal significantly reduced the overall rolling velocity of Jurkat cells relative to that measured on fucosylated GlyCAM-1/IgG over a wide range of sheer stresses (Fig. 3). (nih.gov)
  • The non polar n-hexane extract significantly reduced the number of viable cells and cell proliferation percentage but induced the apoptosis haphazardly. (academicjournals.org)
  • We found that the response of Jurkat cells to hypotonic stress was significantly altered. (eur.nl)
  • Although incubation of Jkt cells with supernatant from non-activated HFRPE cells also showed similar results, the kinetics of procaspase and PARP cleavage showed significantly higher activity in IFN-g activated supernatant. (arvojournals.org)
  • Moreover, overexpression of AEG-1 significantly inversed 3-MA induced-changes in cell proliferation and apoptosis of Hut-78 cells exposed to ADM in hypoxia. (springer.com)
  • In the present study, we found that (-)-epigallocatechin-3-gallate (EGCG) significantly up-regulated the mRNA expression of the Th1/Th2 cytokines including IL-2, IFN-γ, IL-5 and IL-13 in Jurkat T cells. (elsevier.com)
  • Since MTX prevents tumor cells from proliferating by inhibiting dihydrofolate reductase (DHFR), DHFR expression is a key determinant of resistance to MTX in malignant hematological tumor cells. (nih.gov)
  • The xCELLigence system is a new technological approach that allows the real-time cell analysis of adherent tumor cells. (nih.gov)
  • We hypothesize that IL13Rα2-specific CTL clones, delivered in a locoregional fashion to the CNS, will be uniquely suited to eradicate a dispersed population of invasive glioma tumor cells. (aacrjournals.org)
  • The experimental results were fitted using a two-parameter transport numeric model to calculate the Jurkat cell membrane permeability to water and DMSO at room temperature (22 °C). This model and the calculated parameters can help scientists optimize the cryopreservation protocol for any cell type with optimal cryoprotective agents and cooling rate for future experiments. (mdpi.com)