A CELL LINE derived from human T-CELL LEUKEMIA and used to determine the mechanism of differential susceptibility to anti-cancer drugs and radiation.
Lymphocytes responsible for cell-mediated immunity. Two types have been identified - cytotoxic (T-LYMPHOCYTES, CYTOTOXIC) and helper T-lymphocytes (T-LYMPHOCYTES, HELPER-INDUCER). They are formed when lymphocytes circulate through the THYMUS GLAND and differentiate to thymocytes. When exposed to an antigen, they divide rapidly and produce large numbers of new T cells sensitized to that antigen.
A tumor necrosis factor receptor subtype found in a variety of tissues and on activated LYMPHOCYTES. It has specificity for FAS LIGAND and plays a role in regulation of peripheral immune responses and APOPTOSIS. Multiple isoforms of the protein exist due to multiple ALTERNATIVE SPLICING. The activated receptor signals via a conserved death domain that associates with specific TNF RECEPTOR-ASSOCIATED FACTORS in the CYTOPLASM.
A malignant disease of the T-LYMPHOCYTES in the bone marrow, thymus, and/or blood.
One of the mechanisms by which CELL DEATH occurs (compare with NECROSIS and AUTOPHAGOCYTOSIS). Apoptosis is the mechanism responsible for the physiological deletion of cells and appears to be intrinsically programmed. It is characterized by distinctive morphologic changes in the nucleus and cytoplasm, chromatin cleavage at regularly spaced sites, and the endonucleolytic cleavage of genomic DNA; (DNA FRAGMENTATION); at internucleosomal sites. This mode of cell death serves as a balance to mitosis in regulating the size of animal tissues and in mediating pathologic processes associated with tumor growth.
A family of intracellular CYSTEINE ENDOPEPTIDASES that play a role in regulating INFLAMMATION and APOPTOSIS. They specifically cleave peptides at a CYSTEINE amino acid that follows an ASPARTIC ACID residue. Caspases are activated by proteolytic cleavage of a precursor form to yield large and small subunits that form the enzyme. Since the cleavage site within precursors matches the specificity of caspases, sequential activation of precursors by activated caspases can occur.
A transmembrane protein belonging to the tumor necrosis factor superfamily that was originally discovered on cells of the lymphoid-myeloid lineage, including activated T-LYMPHOCYTES and NATURAL KILLER CELLS. It plays an important role in immune homeostasis and cell-mediated toxicity by binding to the FAS RECEPTOR and triggering APOPTOSIS.
A short pro-domain caspase that plays an effector role in APOPTOSIS. It is activated by INITIATOR CASPASES such as CASPASE 9. Isoforms of this protein exist due to multiple alternative splicing of its MESSENGER RNA.
Established cell cultures that have the potential to propagate indefinitely.
Molecules on the surface of T-lymphocytes that recognize and combine with antigens. The receptors are non-covalently associated with a complex of several polypeptides collectively called CD3 antigens (ANTIGENS, CD3). Recognition of foreign antigen and the major histocompatibility complex is accomplished by a single heterodimeric antigen-receptor structure, composed of either alpha-beta (RECEPTORS, ANTIGEN, T-CELL, ALPHA-BETA) or gamma-delta (RECEPTORS, ANTIGEN, T-CELL, GAMMA-DELTA) chains.
A soluble substance elaborated by antigen- or mitogen-stimulated T-LYMPHOCYTES which induces DNA synthesis in naive lymphocytes.
A long pro-domain caspase that contains a death effector domain in its pro-domain region. Caspase 8 plays a role in APOPTOSIS by cleaving and activating EFFECTOR CASPASES. Activation of this enzyme can occur via the interaction of its N-terminal death effector domain with DEATH DOMAIN RECEPTOR SIGNALING ADAPTOR PROTEINS.
This enzyme is a lymphoid-specific src family tyrosine kinase that is critical for T-cell development and activation. Lck is associated with the cytoplasmic domains of CD4, CD8 and the beta-chain of the IL-2 receptor, and is thought to be involved in the earliest steps of TCR-mediated T-cell activation.
The uptake of naked or purified DNA by CELLS, usually meaning the process as it occurs in eukaryotic cells. It is analogous to bacterial transformation (TRANSFORMATION, BACTERIAL) and both are routinely employed in GENE TRANSFER TECHNIQUES.
A long pro-domain caspase that contains a caspase recruitment domain in its pro-domain region. Caspase 9 is activated during cell stress by mitochondria-derived proapoptotic factors and by CARD SIGNALING ADAPTOR PROTEINS such as APOPTOTIC PROTEASE-ACTIVATING FACTOR 1. It activates APOPTOSIS by cleaving and activating EFFECTOR CASPASES.
The intracellular transfer of information (biological activation/inhibition) through a signal pathway. In each signal transduction system, an activation/inhibition signal from a biologically active molecule (hormone, neurotransmitter) is mediated via the coupling of a receptor/enzyme to a second messenger system or to an ion channel. Signal transduction plays an important role in activating cellular functions, cell differentiation, and cell proliferation. Examples of signal transduction systems are the GAMMA-AMINOBUTYRIC ACID-postsynaptic receptor-calcium ion channel system, the receptor-mediated T-cell activation pathway, and the receptor-mediated activation of phospholipases. Those coupled to membrane depolarization or intracellular release of calcium include the receptor-mediated activation of cytotoxic functions in granulocytes and the synaptic potentiation of protein kinase activation. Some signal transduction pathways may be part of larger signal transduction pathways; for example, protein kinase activation is part of the platelet activation signal pathway.
Cells grown in vitro from neoplastic tissue. If they can be established as a TUMOR CELL LINE, they can be propagated in cell culture indefinitely.
A phorbol ester found in CROTON OIL with very effective tumor promoting activity. It stimulates the synthesis of both DNA and RNA.
Morphologic alteration of small B LYMPHOCYTES or T LYMPHOCYTES in culture into large blast-like cells able to synthesize DNA and RNA and to divide mitotically. It is induced by INTERLEUKINS; MITOGENS such as PHYTOHEMAGGLUTININS, and by specific ANTIGENS. It may also occur in vivo as in GRAFT REJECTION.
A family of transcription factors characterized by the presence of highly conserved calcineurin- and DNA-binding domains. NFAT proteins are activated in the CYTOPLASM by the calcium-dependent phosphatase CALCINEURIN. They transduce calcium signals to the nucleus where they can interact with TRANSCRIPTION FACTOR AP-1 or NF-KAPPA B and initiate GENETIC TRANSCRIPTION of GENES involved in CELL DIFFERENTIATION and development. NFAT proteins stimulate T-CELL activation through the induction of IMMEDIATE-EARLY GENES such as INTERLEUKIN-2.
Conversion of an inactive form of an enzyme to one possessing metabolic activity. It includes 1, activation by ions (activators); 2, activation by cofactors (coenzymes); and 3, conversion of an enzyme precursor (proenzyme or zymogen) to an active enzyme.
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
Endogenous and exogenous compounds and that either inhibit CASPASES or prevent their activation.
Mucoproteins isolated from the kidney bean (Phaseolus vulgaris); some of them are mitogenic to lymphocytes, others agglutinate all or certain types of erythrocytes or lymphocytes. They are used mainly in the study of immune mechanisms and in cell culture.
Inhibitors of SERINE ENDOPEPTIDASES and sulfhydryl group-containing enzymes. They act as alkylating agents and are known to interfere in the translation process.
A signal-transducing adaptor protein that associates with TNF RECEPTOR complexes. It contains a death effector domain that can interact with death effector domains found on INITIATOR CASPASES such as CASPASE 8 and CASPASE 10. Activation of CASPASES via interaction with this protein plays a role in the signaling cascade that leads to APOPTOSIS.
Exogenous and endogenous compounds which inhibit CYSTEINE ENDOPEPTIDASES.
Technique using an instrument system for making, processing, and displaying one or more measurements on individual cells obtained from a cell suspension. Cells are usually stained with one or more fluorescent dyes specific to cell components of interest, e.g., DNA, and fluorescence of each cell is measured as it rapidly transverses the excitation beam (laser or mercury arc lamp). Fluorescence provides a quantitative measure of various biochemical and biophysical properties of the cell, as well as a basis for cell sorting. Other measurable optical parameters include light absorption and light scattering, the latter being applicable to the measurement of cell size, shape, density, granularity, and stain uptake.
Anti-CD3 monoclonal antibody that exerts immunosuppressive effects by inducing peripheral T-cell depletion and modulation of the T-cell receptor complex (CD3/Ti).
Complex of at least five membrane-bound polypeptides in mature T-lymphocytes that are non-covalently associated with one another and with the T-cell receptor (RECEPTORS, ANTIGEN, T-CELL). The CD3 complex includes the gamma, delta, epsilon, zeta, and eta chains (subunits). When antigen binds to the T-cell receptor, the CD3 complex transduces the activating signals to the cytoplasm of the T-cell. The CD3 gamma and delta chains (subunits) are separate from and not related to the gamma/delta chains of the T-cell receptor (RECEPTORS, ANTIGEN, T-CELL, GAMMA-DELTA).
Glycoproteins found on the membrane or surface of cells.
The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.
The introduction of a phosphoryl group into a compound through the formation of an ester bond between the compound and a phosphorus moiety.
Antigens expressed on the cell membrane of T-lymphocytes during differentiation, activation, and normal and neoplastic transformation. Their phenotypic characterization is important in differential diagnosis and studies of thymic ontogeny and T-cell function.
A sialic acid-rich protein and an integral cell membrane mucin. It plays an important role in activation of T-LYMPHOCYTES.
Glycoprotein members of the immunoglobulin superfamily which participate in T-cell adhesion and activation. They are expressed on most peripheral T-lymphocytes, natural killer cells, and thymocytes, and function as co-receptors or accessory molecules in the T-cell receptor complex.
A human cell line established from a diffuse histiocytic lymphoma (HISTIOCYTIC LYMPHOMA, DIFFUSE) and displaying many monocytic characteristics. It serves as an in vitro model for MONOCYTE and MACROPHAGE differentiation.
Compounds or agents that combine with an enzyme in such a manner as to prevent the normal substrate-enzyme combination and the catalytic reaction.
Ubiquitous, inducible, nuclear transcriptional activator that binds to enhancer elements in many different cell types and is activated by pathogenic stimuli. The NF-kappa B complex is a heterodimer composed of two DNA-binding subunits: NF-kappa B1 and relA.
A broad category of carrier proteins that play a role in SIGNAL TRANSDUCTION. They generally contain several modular domains, each of which having its own binding activity, and act by forming complexes with other intracellular-signaling molecules. Signal-transducing adaptor proteins lack enzyme activity, however their activity can be modulated by other signal-transducing enzymes
A promyelocytic cell line derived from a patient with ACUTE PROMYELOCYTIC LEUKEMIA. HL-60 cells lack specific markers for LYMPHOID CELLS but express surface receptors for FC FRAGMENTS and COMPLEMENT SYSTEM PROTEINS. They also exhibit phagocytic activity and responsiveness to chemotactic stimuli. (From Hay et al., American Type Culture Collection, 7th ed, pp127-8)
Semiautonomous, self-reproducing organelles that occur in the cytoplasm of all cells of most, but not all, eukaryotes. Each mitochondrion is surrounded by a double limiting membrane. The inner membrane is highly invaginated, and its projections are called cristae. Mitochondria are the sites of the reactions of oxidative phosphorylation, which result in the formation of ATP. They contain distinctive RIBOSOMES, transfer RNAs (RNA, TRANSFER); AMINO ACYL T RNA SYNTHETASES; and elongation and termination factors. Mitochondria depend upon genes within the nucleus of the cells in which they reside for many essential messenger RNAs (RNA, MESSENGER). Mitochondria are believed to have arisen from aerobic bacteria that established a symbiotic relationship with primitive protoeukaryotes. (King & Stansfield, A Dictionary of Genetics, 4th ed)
Membrane proteins encoded by the BCL-2 GENES and serving as potent inhibitors of cell death by APOPTOSIS. The proteins are found on mitochondrial, microsomal, and NUCLEAR MEMBRANE sites within many cell types. Overexpression of bcl-2 proteins, due to a translocation of the gene, is associated with follicular lymphoma.
Any of the processes by which nuclear, cytoplasmic, or intercellular factors influence the differential control (induction or repression) of gene action at the level of transcription or translation.
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
A basic element found in nearly all organized tissues. It is a member of the alkaline earth family of metals with the atomic symbol Ca, atomic number 20, and atomic weight 40. Calcium is the most abundant mineral in the body and combines with phosphorus to form calcium phosphate in the bones and teeth. It is essential for the normal functioning of nerves and muscles and plays a role in blood coagulation (as factor IV) and in many enzymatic processes.
Differentiation antigens residing on mammalian leukocytes. CD stands for cluster of differentiation, which refers to groups of monoclonal antibodies that show similar reactivity with certain subpopulations of antigens of a particular lineage or differentiation stage. The subpopulations of antigens are also known by the same CD designation.
RNA sequences that serve as templates for protein synthesis. Bacterial mRNAs are generally primary transcripts in that they do not require post-transcriptional processing. Eukaryotic mRNA is synthesized in the nucleus and must be exported to the cytoplasm for translation. Most eukaryotic mRNAs have a sequence of polyadenylic acid at the 3' end, referred to as the poly(A) tail. The function of this tail is not known for certain, but it may play a role in the export of mature mRNA from the nucleus as well as in helping stabilize some mRNA molecules by retarding their degradation in the cytoplasm.
Costimulatory T-LYMPHOCYTE receptors that have specificity for CD80 ANTIGEN and CD86 ANTIGEN. Activation of this receptor results in increased T-cell proliferation, cytokine production and promotion of T-cell survival.
Splitting the DNA into shorter pieces by endonucleolytic DNA CLEAVAGE at multiple sites. It includes the internucleosomal DNA fragmentation, which along with chromatin condensation, are considered to be the hallmarks of APOPTOSIS.
DNA sequences which are recognized (directly or indirectly) and bound by a DNA-dependent RNA polymerase during the initiation of transcription. Highly conserved sequences within the promoter include the Pribnow box in bacteria and the TATA BOX in eukaryotes.
A non-essential amino acid. In animals it is synthesized from PHENYLALANINE. It is also the precursor of EPINEPHRINE; THYROID HORMONES; and melanin.
Proteins which bind to DNA. The family includes proteins which bind to both double- and single-stranded DNA and also includes specific DNA binding proteins in serum which can be used as markers for malignant diseases.
The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.
Molecule composed of the non-covalent association of the T-cell antigen receptor (RECEPTORS, ANTIGEN, T-CELL) with the CD3 complex (ANTIGENS, CD3). This association is required for the surface expression and function of both components. The molecule consists of up to seven chains: either the alpha/beta or gamma/delta chains of the T-cell receptor, and four or five chains in the CD3 complex.
Derivatives of phosphatidic acids in which the phosphoric acid is bound in ester linkage to a serine moiety. Complete hydrolysis yields 1 mole of glycerol, phosphoric acid and serine and 2 moles of fatty acids.
Antibodies produced by a single clone of cells.
A progressive, malignant disease of the blood-forming organs, characterized by distorted proliferation and development of leukocytes and their precursors in the blood and bone marrow. Leukemias were originally termed acute or chronic based on life expectancy but now are classified according to cellular maturity. Acute leukemias consist of predominately immature cells; chronic leukemias are composed of more mature cells. (From The Merck Manual, 2006)
The biosynthesis of RNA carried out on a template of DNA. The biosynthesis of DNA from an RNA template is called REVERSE TRANSCRIPTION.
Transport proteins that carry specific substances in the blood or across cell membranes.
A group of heterogeneous lymphoid tumors representing malignant transformations of T-lymphocytes.
The relationship between the dose of an administered drug and the response of the organism to the drug.
Products of proto-oncogenes. Normally they do not have oncogenic or transforming properties, but are involved in the regulation or differentiation of cell growth. They often have protein kinase activity.
The process in which substances, either endogenous or exogenous, bind to proteins, peptides, enzymes, protein precursors, or allied compounds. Specific protein-binding measures are often used as assays in diagnostic assessments.
A protein of the annexin family isolated from human PLACENTA and other tissues. It inhibits cytosolic PHOSPHOLIPASE A2, and displays anticoagulant activity.
Proteins found in the nucleus of a cell. Do not confuse with NUCLEOPROTEINS which are proteins conjugated with nucleic acids, that are not necessarily present in the nucleus.
Members of the class of neutral glycosphingolipids. They are the basic units of SPHINGOLIPIDS. They are sphingoids attached via their amino groups to a long chain fatty acyl group. They abnormally accumulate in FABRY DISEASE.
Endogenous substances, usually proteins, which are effective in the initiation, stimulation, or termination of the genetic transcription process.
Regulatory sequences important for viral replication that are located on each end of the HIV genome. The LTR includes the HIV ENHANCER, promoter, and other sequences. Specific regions in the LTR include the negative regulatory element (NRE), NF-kappa B binding sites , Sp1 binding sites, TATA BOX, and trans-acting responsive element (TAR). The binding of both cellular and viral proteins to these regions regulates HIV transcription.
The type species of LENTIVIRUS and the etiologic agent of AIDS. It is characterized by its cytopathic effect and affinity for the T4-lymphocyte.
White blood cells formed in the body's lymphoid tissue. The nucleus is round or ovoid with coarse, irregularly clumped chromatin while the cytoplasm is typically pale blue with azurophilic (if any) granules. Most lymphocytes can be classified as either T or B (with subpopulations of each), or NATURAL KILLER CELLS.
Glycoproteins with a wide distribution on hematopoietic and non-hematopoietic cells and strongly expressed on macrophages. CD58 mediates cell adhesion by binding to CD2; (ANTIGENS, CD2); and this enhances antigen-specific T-cell activation.
A negative regulatory effect on physiological processes at the molecular, cellular, or systemic level. At the molecular level, the major regulatory sites include membrane receptors, genes (GENE EXPRESSION REGULATION), mRNAs (RNA, MESSENGER), and proteins.
The span of viability of a cell characterized by the capacity to perform certain functions such as metabolism, growth, reproduction, some form of responsiveness, and adaptability.
A semisynthetic derivative of PODOPHYLLOTOXIN that exhibits antitumor activity. Etoposide inhibits DNA synthesis by forming a complex with topoisomerase II and DNA. This complex induces breaks in double stranded DNA and prevents repair by topoisomerase II binding. Accumulated breaks in DNA prevent entry into the mitotic phase of cell division, and lead to cell death. Etoposide acts primarily in the G2 and S phases of the cell cycle.
A member of the Bcl-2 protein family that reversibly binds MEMBRANES. It is a pro-apoptotic protein that is activated by caspase cleavage.
The parts of a macromolecule that directly participate in its specific combination with another molecule.
Aggressive T-Cell malignancy with adult onset, caused by HUMAN T-LYMPHOTROPIC VIRUS 1. It is endemic in Japan, the Caribbean basin, Southeastern United States, Hawaii, and parts of Central and South America and sub-Saharan Africa.
Recombinant proteins produced by the GENETIC TRANSLATION of fused genes formed by the combination of NUCLEIC ACID REGULATORY SEQUENCES of one or more genes with the protein coding sequences of one or more genes.
An indolocarbazole that is a potent PROTEIN KINASE C inhibitor which enhances cAMP-mediated responses in human neuroblastoma cells. (Biochem Biophys Res Commun 1995;214(3):1114-20)
Processes that stimulate the GENETIC TRANSCRIPTION of a gene or set of genes.
An eph family receptor found primarily in BRAIN and THYMUS. The EphB6 receptor is unusual in that its tyrosine kinase domain shares little homology with other members of this class. The unusual tyrosine kinase domain of this receptor appears to result in its lack of tyrosine kinase activity.
A superfamily of PROTEIN-SERINE-THREONINE KINASES that are activated by diverse stimuli via protein kinase cascades. They are the final components of the cascades, activated by phosphorylation by MITOGEN-ACTIVATED PROTEIN KINASE KINASES, which in turn are activated by mitogen-activated protein kinase kinase kinases (MAP KINASE KINASE KINASES).
The rate dynamics in chemical or physical systems.
The first continuously cultured human malignant CELL LINE, derived from the cervical carcinoma of Henrietta Lacks. These cells are used for VIRUS CULTIVATION and antitumor drug screening assays.
A divalent calcium ionophore that is widely used as a tool to investigate the role of intracellular calcium in cellular processes.
Proto-oncogene proteins that are guanine nucleotide exchange factors for RHO GTPASES. They also function as signal transducing adaptor proteins.
A protein tyrosine kinase that is required for T-CELL development and T-CELL ANTIGEN RECEPTOR function.
A strain of PRIMATE T-LYMPHOTROPIC VIRUS 1 isolated from mature T4 cells in patients with T-lymphoproliferation malignancies. It causes adult T-cell leukemia (LEUKEMIA-LYMPHOMA, T-CELL, ACUTE, HTLV-I-ASSOCIATED), T-cell lymphoma (LYMPHOMA, T-CELL), and is involved in mycosis fungoides, SEZARY SYNDROME and tropical spastic paraparesis (PARAPARESIS, TROPICAL SPASTIC).
The lipid- and protein-containing, selectively permeable membrane that surrounds the cytoplasm in prokaryotic and eukaryotic cells.
An alkaloid obtained from the betel nut (Areca catechu), fruit of a palm tree. It is an agonist at both muscarinic and nicotinic acetylcholine receptors. It is used in the form of various salts as a ganglionic stimulant, a parasympathomimetic, and a vermifuge, especially in veterinary practice. It has been used as a euphoriant in the Pacific Islands.
A large group of proteins that control APOPTOSIS. This family of proteins includes many ONCOGENE PROTEINS as well as a wide variety of classes of INTRACELLULAR SIGNALING PEPTIDES AND PROTEINS such as CASPASES.
A water-soluble, enzyme co-factor present in minute amounts in every living cell. It occurs mainly bound to proteins or polypeptides and is abundant in liver, kidney, pancreas, yeast, and milk.
An enzyme that catalyzes the acetylation of chloramphenicol to yield chloramphenicol 3-acetate. Since chloramphenicol 3-acetate does not bind to bacterial ribosomes and is not an inhibitor of peptidyltransferase, the enzyme is responsible for the naturally occurring chloramphenicol resistance in bacteria. The enzyme, for which variants are known, is found in both gram-negative and gram-positive bacteria. EC
Proteins encoded by the TAT GENES of the HUMAN IMMUNODEFICIENCY VIRUS.
Cytochromes of the c type that are found in eukaryotic MITOCHONDRIA. They serve as redox intermediates that accept electrons from MITOCHONDRIAL ELECTRON TRANSPORT COMPLEX III and transfer them to MITOCHONDRIAL ELECTRON TRANSPORT COMPLEX IV.
Transcriptional trans-acting proteins of the promoter elements found in the long terminal repeats (LTR) of HUMAN T-LYMPHOTROPIC VIRUS 1 and HUMAN T-LYMPHOTROPIC VIRUS 2. The tax (trans-activator x; x is undefined) proteins act by binding to enhancer elements in the LTR.
An serine-threonine protein kinase that requires the presence of physiological concentrations of CALCIUM and membrane PHOSPHOLIPIDS. The additional presence of DIACYLGLYCEROLS markedly increases its sensitivity to both calcium and phospholipids. The sensitivity of the enzyme can also be increased by PHORBOL ESTERS and it is believed that protein kinase C is the receptor protein of tumor-promoting phorbol esters.
A subgroup of mitogen-activated protein kinases that activate TRANSCRIPTION FACTOR AP-1 via the phosphorylation of C-JUN PROTEINS. They are components of intracellular signaling pathways that regulate CELL PROLIFERATION; APOPTOSIS; and CELL DIFFERENTIATION.
Proteins which are found in membranes including cellular and intracellular membranes. They consist of two types, peripheral and integral proteins. They include most membrane-associated enzymes, antigenic proteins, transport proteins, and drug, hormone, and lectin receptors.
Proteins prepared by recombinant DNA technology.
A sesquiterpene lactone found in roots of THAPSIA. It inhibits CA(2+)-TRANSPORTING ATPASE mediated uptake of CALCIUM into SARCOPLASMIC RETICULUM.
Trans-acting transcription factors produced by retroviruses such as HIV. They are nuclear proteins whose expression is required for viral replication. The tat protein stimulates LONG TERMINAL REPEAT-driven RNA synthesis for both viral regulatory and viral structural proteins. tat stands for trans-activation of transcription.
A long pro-domain caspase that contains a caspase recruitment domain in its pro-domain region. Activation of this enzyme can occur via the interaction of its caspase recruitment domain with CARD SIGNALING ADAPTOR PROTEINS. Caspase 2 plays a role in APOPTOSIS by cleaving and activating effector pro-caspases. Several isoforms of this protein exist due to multiple alternative splicing of its MESSENGER RNA.
A group of cytochromes with covalent thioether linkages between either or both of the vinyl side chains of protoheme and the protein. (Enzyme Nomenclature, 1992, p539)
A multiprotein complex composed of the products of c-jun and c-fos proto-oncogenes. These proteins must dimerize in order to bind to the AP-1 recognition site, also known as the TPA-responsive element (TRE). AP-1 controls both basal and inducible transcription of several genes.
A class of compounds composed of repeating 5-carbon units of HEMITERPENES.
Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others.
A plant genus of the family CELASTRACEAE that is a source of triterpenoids and diterpene epoxides such as triptolide.
The fluid inside CELLS.
The termination of the cell's ability to carry out vital functions such as metabolism, growth, reproduction, responsiveness, and adaptability.
A phosphoinositide phospholipase C subtype that is primarily regulated by PROTEIN-TYROSINE KINASES. It is structurally related to PHOSPHOLIPASE C DELTA with the addition of SRC HOMOLOGY DOMAINS and pleckstrin homology domains located between two halves of the CATALYTIC DOMAIN.
Serum glycoprotein produced by activated MACROPHAGES and other mammalian MONONUCLEAR LEUKOCYTES. It has necrotizing activity against tumor cell lines and increases ability to reject tumor transplants. Also known as TNF-alpha, it is only 30% homologous to TNF-beta (LYMPHOTOXIN), but they share TNF RECEPTORS.
A critical subpopulation of T-lymphocytes involved in the induction of most immunological functions. The HIV virus has selective tropism for the T4 cell which expresses the CD4 phenotypic marker, a receptor for HIV. In fact, the key element in the profound immunosuppression seen in HIV infection is the depletion of this subset of T-lymphocytes.
Within a eukaryotic cell, a membrane-limited body which contains chromosomes and one or more nucleoli (CELL NUCLEOLUS). The nuclear membrane consists of a double unit-type membrane which is perforated by a number of pores; the outermost membrane is continuous with the ENDOPLASMIC RETICULUM. A cell may contain more than one nucleus. (From Singleton & Sainsbury, Dictionary of Microbiology and Molecular Biology, 2d ed)

The hematopoietic-specific adaptor protein gads functions in T-cell signaling via interactions with the SLP-76 and LAT adaptors. (1/6443)

BACKGROUND: The adaptor protein Gads is a Grb2-related protein originally identified on the basis of its interaction with the tyrosine-phosphorylated form of the docking protein Shc. Gads protein expression is restricted to hematopoietic tissues and cell lines. Gads contains a Src homology 2 (SH2) domain, which has previously been shown to have a similar binding specificity to that of Grb2. Gads also possesses two SH3 domains, but these have a distinct binding specificity to those of Grb2, as Gads does not bind to known Grb2 SH3 domain targets. Here, we investigated whether Gads is involved in T-cell signaling. RESULTS: We found that Gads is highly expressed in T cells and that the SLP-76 adaptor protein is a major Gads-associated protein in vivo. The constitutive interaction between Gads and SLP-76 was mediated by the carboxy-terminal SH3 domain of Gads and a 20 amino-acid proline-rich region in SLP-76. Gads also coimmunoprecipitated the tyrosine-phosphorylated form of the linker for activated T cells (LAT) adaptor protein following cross-linking of the T-cell receptor; this interaction was mediated by the Gads SH2 domain. Overexpression of Gads and SLP-76 resulted in a synergistic augmentation of T-cell signaling, as measured by activation of nuclear factor of activated T cells (NFAT), and this cooperation required a functional Gads SH2 domain. CONCLUSIONS: These results demonstrate that Gads plays an important role in T-cell signaling via its association with SLP-76 and LAT. Gads may promote cross-talk between the LAT and SLP-76 signaling complexes, thereby coupling membrane-proximal events to downstream signaling pathways.  (+info)

Tyrosine phosphorylation and complex formation of Cbl-b upon T cell receptor stimulation. (2/6443)

Cbl-b, a mammalian homolog of Cbl, consists of an N-terminal region (Cbl-b-N) highly homologous to oncogenic v-Cbl, a Ring finger, and a C-terminal region containing multiple proline-rich stretches and potential tyrosine phosphorylation sites. In the present study, we demonstrate that upon engagement of the T cell receptor (TCR), endogenous Cbl-b becomes rapidly tyrosine-phosphorylated. In heterogeneous COS-1 cells, Cbl-b was phosphorylated on tyrosine residues by both Syk- (Syk/Zap-70) and Src- (Fyn/Lck) family kinases, with Syk kinase inducing the most prominent effect. Syk associates and phosphorylates Cbl-b in Jurkat T cells. A Tyr-316 Cbl-binding site in Syk was required for the association with and for the maximal tyrosine phosphorylation of Cbl-b. Mutation at a loss-of-function site (Gly-298) in Cbl-b-N disrupts its interaction with Syk. Cbl-b constitutively binds Grb2 and becomes associated with Crk-L upon TCR stimulation. The Grb2- and the Crk-L-binding regions were mapped to the C-terminus of Cbl-b. The Crk-L-binding sites were further determined to be Y655DVP and Y709KIP, with the latter being the primary binding site. Taken together, these results implicate that Cbl-b is involved in TCR-mediated intracellular signaling pathways.  (+info)

Reactive oxygen intermediate-dependent NF-kappaB activation by interleukin-1beta requires 5-lipoxygenase or NADPH oxidase activity. (3/6443)

We previously reported that the role of reactive oxygen intermediates (ROIs) in NF-kappaB activation by proinflammatory cytokines was cell specific. However, the sources for ROIs in various cell types are yet to be determined and might include 5-lipoxygenase (5-LOX) and NADPH oxidase. 5-LOX and 5-LOX activating protein (FLAP) are coexpressed in lymphoid cells but not in monocytic or epithelial cells. Stimulation of lymphoid cells with interleukin-1beta (IL-1beta) led to ROI production and NF-kappaB activation, which could both be blocked by antioxidants or FLAP inhibitors, confirming that 5-LOX was the source of ROIs and was required for NF-kappaB activation in these cells. IL-1beta stimulation of epithelial cells did not generate any ROIs and NF-kappaB induction was not influenced by 5-LOX inhibitors. However, reintroduction of a functional 5-LOX system in these cells allowed ROI production and 5-LOX-dependent NF-kappaB activation. In monocytic cells, IL-1beta treatment led to a production of ROIs which is independent of the 5-LOX enzyme but requires the NADPH oxidase activity. This pathway involves the Rac1 and Cdc42 GTPases, two enzymes which are not required for NF-kappaB activation by IL-1beta in epithelial cells. In conclusion, three different cell-specific pathways lead to NF-kappaB activation by IL-1beta: a pathway dependent on ROI production by 5-LOX in lymphoid cells, an ROI- and 5-LOX-independent pathway in epithelial cells, and a pathway requiring ROI production by NADPH oxidase in monocytic cells.  (+info)

Jun kinase phosphorylates and regulates the DNA binding activity of an octamer binding protein, T-cell factor beta1. (4/6443)

POU domain proteins have been implicated as key regulators during development and lymphocyte activation. The POU domain protein T-cell factor beta1 (TCFbeta1), which binds octamer and octamer-related sequences, is a potent transactivator. In this study, we showed that TCFbeta1 is phosphorylated following activation via the T-cell receptor or by stress-induced signals. Phosphorylation of TCFbeta1 occurred predominantly at serine and threonine residues. Signals which upregulate Jun kinase (JNK)/stress-activated protein kinase activity also lead to association of JNK with TCFbeta1. JNK associates with the activation domain of TCFbeta1 and phosphorylates its DNA binding domain. The phosphorylation of recombinant TCFbeta1 by recombinant JNK enhances the ability of TCFbeta1 to bind to a consensus octamer motif. Consistent with this conclusion, TCFbeta1 upregulates reporter gene transcription in an activation- and JNK-dependent manner. In addition, inhibition of JNK activity by catalytically inactive MEKK (in which methionine was substituted for the lysine at position 432) also inhibits the ability of TCFbeta1 to drive inducible transcription from the interleukin-2 promoter. These results suggest that stress-induced signals and T-cell activation induce JNK, which then acts on multiple cis sequences by modulating distinct transactivators like c-Jun and TCFbeta1. This demonstrates a coupling between the JNK activation pathway and POU domain proteins and implicates TCFbeta1 as a physiological target in the JNK signal transduction pathway leading to coordinated biological responses.  (+info)

Activation-dependent transcriptional regulation of the human Fas promoter requires NF-kappaB p50-p65 recruitment. (5/6443)

Fas (CD95) and Fas ligand (CD95L) are an interacting receptor-ligand pair required for immune homeostasis. Lymphocyte activation results in the upregulation of Fas expression and the acquisition of sensitivity to FasL-mediated apoptosis. Although Fas upregulation is central to the preservation of immunologic tolerance, little is known about the molecular machinery underlying this process. To investigate the events involved in activation-induced Fas upregulation, we have examined mRNA accumulation, fas promoter activity, and protein expression in the Jurkat T-cell line treated with phorbol myristate acetate and ionomycin (P/I), pharmacological mimics of T-cell receptor activation. Although resting Jurkat cells express Fas, Fas mRNA was induced approximately 10-fold in 2 h upon P/I stimulation. Using sequential deletion mutants of the human fas promoter in transient transfection assays, we identified a 47-bp sequence (positions -306 to -260 relative to the ATG) required for activation-driven fas upregulation. Sequence analysis revealed the presence of a previously unrecognized composite binding site for both the Sp1 and NF-kappaB transcription factors at positions -295 to -286. Electrophoretic mobility shift assay (EMSA) and supershift analyses of this region documented constitutive binding of Sp1 in unactivated nuclear extracts and inducible binding of p50-p65 NF-kappaB heterodimers after P/I activation. Sp1 and NF-kappaB transcription factor binding was shown to be mutually exclusive by EMSA displacement studies with purified recombinant Sp1 and recombinant p50. The functional contribution of the kappaB-Sp1 composite site in P/I-inducible fas promoter activation was verified by using kappaB-Sp1 concatamers (-295 to -286) in a thymidine kinase promoter-driven reporter construct and native promoter constructs in Jurkat cells overexpressing IkappaB-alpha. Site-directed mutagenesis of the critical guanine nucleotides in the kappaB-Sp1 element documented the essential role of this site in activation-dependent fas promoter induction.  (+info)

Requirement for transcription factor NFAT in interleukin-2 expression. (6/6443)

The nuclear factor of activated T cells (NFAT) transcription factor is implicated in expression of the cytokine interleukin-2 (IL-2). Binding sites for NFAT are located in the IL-2 promoter. Furthermore, pharmacological studies demonstrate that the drug cyclosporin A inhibits both NFAT activation and IL-2 expression. However, targeted disruption of the NFAT1 and NFAT2 genes in mice does not cause decreased IL-2 secretion. The role of NFAT in IL-2 gene expression is therefore unclear. Here we report the construction of a dominant-negative NFAT mutant (dnNFAT) that selectively inhibits NFAT-mediated gene expression. The inhibitory effect of dnNFAT is mediated by suppression of activation-induced nuclear translocation of NFAT. Expression of dnNFAT in cultured T cells caused inhibition of IL-2 promoter activity and decreased expression of IL-2 protein. Similarly, expression of dnNFAT in transgenic mice also caused decreased IL-2 gene expression. These data demonstrate that NFAT is a critical component of the signaling pathway that regulates IL-2 expression.  (+info)

Physical interaction of the bHLH LYL1 protein and NF-kappaB1 p105. (7/6443)

The LYL1 gene was first identified upon the molecular characterization of the t(7;9)(q35;p13) translocation associated with some human T-cell acute leukemias (T-ALLs). In adult tissues, LYL1 expression is restricted to hematopoietic cells with the notable exclusion of the T cell lineage. LYL1 encodes a basic helix-loop-helix (bHLH) protein highly related to TAL-1, whose activation is also associated with a high proportion of human T-ALLs. A yeast two-hybrid system was used to identify proteins that specifically interact with LYL1 and might mediate its activities. We found that p105, the precursor of NF-kappaB1 p50, was the major LYL1-interacting protein in this system. The association between LYL1 and p105 was confirmed both in vitro and in vivo in mammalian cells. Biochemical studies indicated that the interaction was mediated by the bHLH motif of LYL1 and the ankyrin-like motifs of p105. Ectopic expression of LYL1 in a human T cell line caused a significant decrease in NF-kappaB-dependent transcription, associated with a reduced level of NF-kappaB1 proteins.  (+info)

Proteolytic processing of the Alzheimer's disease amyloid precursor protein within its cytoplasmic domain by caspase-like proteases. (8/6443)

Alzheimer's disease is characterized by neurodegeneration and deposition of betaA4, a peptide that is proteolytically released from the amyloid precursor protein (APP). Missense mutations in the genes coding for APP and for the polytopic membrane proteins presenilin (PS) 1 and PS2 have been linked to familial forms of early-onset Alzheimer's disease. Overexpression of presenilins, especially that of PS2, induces increased susceptibility for apoptosis that is even more pronounced in cells expressing presenilin mutants. Additionally, presenilins themselves are targets for activated caspases in apoptotic cells. When we analyzed APP in COS-7 cells overexpressing PS2, we observed proteolytic processing close to the APP carboxyl terminus. Proteolytic conversion was increased in the presence of PS2-I, which encodes one of the known PS2 pathogenic mutations. The same proteolytic processing occurred in cells treated with chemical inducers of apoptosis, suggesting a participation of activated caspases in the carboxyl-terminal truncation of APP. This was confirmed by showing that specific caspase inhibitors blocked the apoptotic conversion of APP. Sequence analysis of the APP cytosolic domain revealed a consensus motif for group III caspases ((IVL)ExD). Mutation of the corresponding Asp664 residue abolished cleavage, thereby identifying APP as a target molecule for caspase-like proteases in the pathways of programmed cellular death.  (+info)

BioAssay record AID 1270344 submitted by ChEMBL: Induction of cell proliferation in human Jurkat T cells at 200 uM after 24 hrs by tryphan blue assay.
A growing body of evidence strongly indicates that both simulated and authentic weightlessness exert a broad range of effects on mammalian tissues and cells, including impairment of immune cell function and increased apoptotic death. We previously reported that microgravity-dependent activation of 5-lipoxygenase (5-LOX) might play a central role in the initiation of apoptosis in human T lymphocytes, suggesting that the upregulation of this enzyme might be (at least in part) responsible for immunodepression observed in astronauts during space flights. Herein, we supplement novel information about the molecular mechanisms underlying microgravity-triggered apoptotic cell death and immune system deregulation, demonstrating that under simulated microgravity human Jurkat T cells increase the content of cytosolic DNA fragments and cytochrome c (typical hallmarks of apoptosis) and have an upregulated expression and activity of |i|µ|/i|-calpain. These events were paralleled by the unbalance of interleukin-
Citation: Macho, A., Blanco-Molina, M., Spagliardi, P., Appendino, G., Bremner, P., Heinrich, M., Fiebich, B. L. and Munoz, E. (2004) Calcium ionophoretic and apoptotic effects of ferutinin in the human Jurkat T-cell line. Biochemical Pharmacology, 68 (5), pp. 875-883. ...
Citation : Macho, A., Blanco-Molina, M., Spagliardi, P., Appendino, G., Bremner, P., Heinrich, M., Fiebich, B. L. and Munoz, E. (2004) Calcium ionophoretic and apoptotic effects of ferutinin in the human Jurkat T-cell line. Biochemical Pharmacology, 68 (5), pp. 875-883. ...
BioAssay record AID 1077914 submitted by ChEMBL: Antiviral activity against HIV1 infected in human Jurkat cells assessed as inhibition of viral replication by CAT gene-based transcomplementation assay.
The Western diet is high in omega-6 fatty acids and low in omega-3 fatty acids. Canola oil contains a healthier omega 3 to omega 6 ratio than corn oil. Jurkat T leukemia cells were treated with free fatty acids mixtures in ratios mimicking that found in commercially available canola oil (7% α-linolenic, 30% linoleic, 54% oleic) or corn oil (59% linoleic, 24% oleic) to determine the cell survival or cell death and changes in expression levels of inflammatory cytokines and receptors following oil treatment. Fatty acid uptake was assessed by gas chromatography. Cell survival and cell death were evaluated by cell cycle analyses, propidium-iodide staining, trypan blue exclusion and phosphatidylserine externalization. mRNA levels of inflammatory cytokines and receptors were assessed by RT-PCR. There was a significant difference in the lipid profiles of the cells after treatment. Differential action of the oils on inflammatory molecules, following treatment at non-cytotoxic levels, indicated that canola oil
Apoptotic cells release the nucleotides ATP and UTP to attract phagocytic cells, which in turn clear the apoptotic cells. Chekeni et al. found that carbenoxolone (CBX), which inhibits connexins (which form gap junctions) and pannexins (which form plasma membrane channels), and probenicid, which is thought to be more specific for pannexins, reduced ATP release from apoptotic Jurkat cells to a similar extent as the caspase inhibitor zVAD (which blocks the release of ATP from apoptotic cells). Small interfering RNAs (siRNAs) directed against pannexin 1 (PANX1) decreased the release of ATP and UTP from apoptotic Jurkat cells but did not prevent apoptosis. Supernatant from PANX1 siRNA-transfected apoptotic cells recruited fewer monocytes in an in vitro assay of cell migration and when placed in a subcutaneous air pouch in mice. In contrast, Jurkat cells stably overexpressing PANX1 released more ATP and UTP, and supernatants from these cells (after apoptosis had been induced) recruited more monocytes ...
Certain clones of Jurkat cells are susceptible to necroptosis, but not all of them. In my experience, the FADD-deficient clone 5C3 is highly susceptible to necroptotic death induced by TNFa. The wild type jurkat cell line A3 is not and the caspase-8 deficient line I9.2 is only mildly susceptible by itself, but, surprisingly, becomes more susceptible upon addition of z-VAD-fmk, suggesting that caspase-8 is not essential to prevent necroptosis in these cells. The RIPK1-deficient jurkat cell line is not susceptible to necroptosis unless reconstituted with RIPK1 harbouring a cleavage site mutation, The parental clone doesnt undergo necroptosis upon TNFa stimulation in the presence of z-VAD-fmk. However, the RIPK1 deficient cells are extremely susceptible to all forms of apoptosis, suggesting either an important role of RIPK1 in preventing apoptosis or that these cells lack another anti-apoptotic factor in addition to RIPK1. These cells were initially generated by selecting randomly mutated jurkat ...
Grape pomace is a rich source of phenolic compounds commonly employed for elaboration of dietary supplements. The aim of the present study was to investigate the anticancer effect of a purified white grape pomace extract (PWGPE) in acute lymphoblastic leukemia Jurkat cells and to characterize the underlying
Creative Biolabs provides Anti-MCC peptide (ADLIAYLKQATK) T Cell Receptor (clone 226), Jurkat Cell Line product for Biopharmaceutical research,preclinical and clinical trials.
Creative Biolabs provides Anti-RAC1 (FSGEHPTV) T Cell Receptor (clone T3), Jurkat Cell Line product for Biopharmaceutical research,preclinical and clinical trials.
The Neo Jurkat cell line was derived by transfecting human Jurkat T cells with the empty pSFFV mammalian expression vector carrying a neomycin-resistant gene.  Stable neomycin (Neo)-resistant single-cell-originated clones were isolated and expanded by selection in medium containing 1 mg/mL G418 for 14 days.
ABSTRACT: RNA interference (RNAi) is a potent gene delivery system for studying the regulation of gene expression in a wide variety of eukaryotic cells. In the present study, different RNAi approaches, namely, synthetic small interfering RNA (siRNA) and plasmid- and lentivirus-based short hairpin RNA (shRNA) were investigated to assess the down-regulation of Nck1 protein efficiency in the Jurkat T cell line. Jurkat T cells treated with these three different systems substantially and specifically reduced the expression of the Nck1 protein but not that of the Nck2 protein. Although the three systems showed a similar Nck1 knockdown efficiency, they led to different T cell activation outcomes. After stimulation, CD69 expression and IL-2 production were impaired in Nck1-siRNA and plasmid-based Nck1-shRNA transfected Jurkat cells. However, these T cell activation outcomes were increased in lentiviral vector based Nck1-shRNA transfected cells. These data suggest that the outcomes from transfection with ...
Show moreBACKGROUND: Kallikrein 6 (KLK6) is a newly identified member of the kallikrein family of secreted serine proteases that prior studies indicate is elevated at sites of central nervous system (CNS) inflammation and which shows regulated expression with T cell activation. Notably, KLK6 is also elevated in the serum of multiple sclerosis (MS) patients however its potential roles in immune function are unknown. Herein we specifically examine whether KLK6 alters immune cell survival and the possible mechanism by which this may occur. METHODOLOGY/PRINCIPAL FINDINGS: Using murine whole splenocyte preparations and the human Jurkat T cell line we demonstrate that KLK6 robustly supports cell survival across a range of cell death paradigms. Recombinant KLK6 was shown to significantly reduce cell death under resting conditions and in response to camptothecin, dexamethasone, staurosporine and Fas-ligand. Moreover, KLK6-over expression in Jurkat T cells was shown to generate parallel pro-survival ...
CD2-mediated T lymphocyte activation requires surface expression of CD3-Ti, the T cell receptor (TCR) for antigen major histocompatibility complex protein. Given the importance of CD3 zeta in TCR signaling, we have directly examined the ability of the CD3 zeta cytoplasmic domain to couple CD2 to intracellular signal transduction pathways. A cDNA encoding a chimeric protein consisting of the human CD3 zeta cytoplasmic domain (amino acid residues 31-142) fused to the CD8 alpha extracellular and transmembrane domains (amino acid residues 1-187) was transfected into a CD2+CD3-CD8- variant of the human T cell line Jurkat. The resulting transfectants expressed the CD8 alpha/CD3 zeta chimeric receptor at the cell surface in the absence of other TCR subunits. Stimulation of these transfectants with anti-T11(2) + anti-T11(3) monoclonal antibodies (mAbs) initiated both a prompt cytosolic free calcium ([Ca2+]i) rise and protein tyrosine kinase activation. Stimulation with either intact anti-T11(2) + ...
Out of the entire population of cells, only the apoptotic Daudi cells immediately decreased CD19 expression via capping, while only the apoptotic Jurkat cells increased CD3 receptor expression 24 h post-exposure. Both receptor changes occurred in a UVA1 dose-dependent manner. We also examined other T-cell receptors, such as CD4, CD25, and CD69, but they did not change for up to 24 h following exposure. During UVA1-triggered immediate apoptosis of Jurkat T cells, IFN- levels increased in a dose-dependent manner at 4 h, but returned to baseline levels at 24 h post-exposure, whereas, there was no significant change in IL-2 at 4 or 24 h. Conclusion ...
Jurkat cell - posted in Tissue and Cell Culture: Hi How can I do to separate live and dead Jurkat cells.? I recently thawed this cell line. I counted with trypan blue and saw many dead cells. Please, help me !!! I do not want to lose these cells.
human Jurkat T cells were treated with increasing concentrations of PDTI and SBTI at different incubation times and the result was assessed employing a old-fashioned tetrazolium centered colorimetric cell proliferation assay. After 24 h incubation at 37 C, 25 cell viability was decreased by uM PDTI in a 30_4%. On another hand, SBTI had a impact, Celecoxib since at 25 uM attention it caused 45_6% cell stability diminution, and even at 2. 5 uM cell viability lowered in a 23_4%. Already after 6 h incubation, 25 uM SBTI caused significant reduction in cell viability, while PDTI expected longer incubation time to make a significant impact. After 24 h of culture, the reduction in cell viability was optimum for both trypsin inhibitors. Longer periods of incubation did not produce significant differences with respect to 24 h. For subsequent studies, designed to comprehend the mechanism through which these trypsin inhibitors decrease stability of Jurkat cells, the PDTI and SBTI levels picked Gene ...
Transmission electron micrograph of a Jurkat T-cell in the early stages of apoptosis, with apoptotic bodies forming. The Jurkat cell line is derived from human T-cell leukemia and used to determine the mechanism of differential susceptibility to anti-cancer drugs and radiation.
The Jurkat cell line was established from the peripheral blood of a 14 year old boy by Schneider et al., and was originally designated JM. The line was cloned from cells obtained from Dr. Kendall Smith and are mycoplasma free. This is a clone of the Jurkat-FHCRC cell line, a derivative of the Jurkat cell line.
The T cell surface molecule CD28 can provide costimulatory signals that permit the full activation of T cells. Here we demonstrate that stimulation of CD28, either by B7, its natural ligand, or by the anti-CD28 monoclonal antibody 9.3, induces an association between CD28 and phosphatidylinositol 3-kinase (PI3-K) in Jurkat T cells, raising the possibility that an interaction with PI3-K contributes to CD28-mediated signaling. To examine the mechanism of the association, we synthesized tyrosine-phosphorylated oligopeptides corresponding to each of the four tyrosines in the CD28 cytoplasmic domain. When added to lysates of B7-stimulated Jurkat cells, the oligopeptide corresponding to Tyr 173 inhibits the coimmunoprecipitation of PI3-K with CD28; the other oligopeptides have no effect. Tyr 173 is contained within the sequence YMNM, a motif that is also found in the platelet-derived growth factor receptor and that, when phosphorylated, forms a high affinity binding site for the p85 subunit of PI3-K. ...
Fig. 2 Functional analysis of TCRs encoded by peripheral blood TFH cells.. (A) Sorting and gating strategy for follicular helper CD4+ T cells. CD3+CD4+CXCR5+CD45RA− PD1++ TFH cells were isolated from PBMCs at day 7 post vaccination from a donor vaccinated with the Fluzone vaccine. (B) Histogram showing expression of the CD69 activation marker on Jurkat cells expressing exogenous TCRs from the vaccinated donor after 24 hours incubation in the presence or absence of autologous DCs and/or Fluzone 2011-2012 vaccine. Numbers of transfected cells are shown. HT-T-1 and HT-T-2, Jurkat cells transfected with TCRβ:α from peripheral TFH cells; RA14, CMV-specific TCRβ:α as a negative control. (C) CDR3 sequences and gene usages of the HT-T-1 clone. ...
Jurkat cells are an immortalized T-lymphocyte cell line, treated with serial dilutions of staurosporine, camptothecin, and etoposide and then assay using Early Tox Live/Dead Assay Kit.
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Western blot analysis of CRNKL1 on human Jurkat cells. The sample was probed with a CRNKL1 polyclonal antibody (Product # PA5-70817) using a primary antibody dilution of 1.0 µg/mL ...
Buy our Jurkat (human T cell lymphoblast-like cell line) whole cell lysate, tumor cell line. ab30128 has been validated in western blot. Abcam now offers…
Store-operated Ca2+ release-activated Ca2+ channels (CRAC) are very high Ca2+ selective channels composed of two protein subunits: STIM1 Ca2+sensor protein placed in the ER and ORAI1 Ca2+ channel protein in plasma membrane. ...
In this report, we describe that T cells can be modified to produce retrovirus after pharmacological induction with a rapalog dimerizer (Fig. 1) ⇓ . Furthermore, these T cells were able to deliver systemically a reporter transgene into a very significant fraction of metastatic tumor cells in the lung and liver (Fig. 2, A and B) ⇓ . When the retrovirus was modified to encode a therapeutic transgene, HSV-tk, we were able to show a significant increase in survival in mice receiving both rapalog to induce viral production and GCV as the cytotoxic prodrug in comparison with animals in which either or both drugs were withheld (Fig. 3A) ⇓ . Interestingly, for achieving a therapeutic effect, the retroviral carrier Jurkat cells had to be tumor-specific. This raises the possibility that CIR signaling results in a benefit over and above that which is produced by nonspecific cells trafficking to tumors and releasing virus. We showed in vivo that tumor specific Jurkat.CEA cells recruit parental Jurkat ...
Chemotherapy, Iron, Administration, Cell, Cell Culture, Cell Viability, Cells, Concentrations, Culture, Drug Targeting, Drugs, Flow Cytometry, Immune System, Jurkat Cell, Jurkat Cells, Leukocytes, Magnetic, Magnetic Field, Mitoxantrone, Nanoparticles
G proteins are membrane-bound molecules involved in coupling of surface receptors with signal transduction effector systems in multiple cell types including T
Z-VAD-FMK is a cell-permeable, irreversible pan-caspase inhibitor, blocks all features of apoptosis in THP.1 and Jurkat T-cells.
Jurkat nuclear extract (1 day growth) for use in WB and EMSA. For studies related to T cells, viral infection (e.g HIV), cytokines, differentiation, and cancer.
IL-2 production by JurkatEGFP and JurkatαCEA-CIR-EGFP cells stimulated either with plastic immobilized anti-CD3 mAb or target cells (E∶T = 1∶1; HeLa or
Protein C Inhibitor (PCI) is a secreted serine protease inhibitor, belonging to the family of serpins. In addition to activated protein C PCI inactivates several other proteases of the coagulation and fibrinolytic systems, suggesting a regulatory role in hemostasis. Glycosaminoglycans and certain negatively charged phospholipids, like phosphatidylserine, bind to PCI and modulate its activity. Phosphatidylerine (PS) is exposed on the surface of apoptotic cells and known as a phagocytosis marker. We hypothesized that PCI might bind to PS exposed on apoptotic cells and thereby influence their removal by phagocytosis. Using Jurkat T-lymphocytes and U937 myeloid cells, we show here that PCI binds to apoptotic cells to a similar extent at the same sites as Annexin V, but in a different manner as compared to live cells (defined spots on ∼10-30% of cells). PCI dose dependently decreased phagocytosis of apoptotic Jurkat cells by U937 macrophages. Moreover, the phagocytosis of PS exposing, activated platelets
Deficient expression of Bak in a clonal Jurkat cell line. (A) Wild-type or the variant Jurkat cell line, Bak−, were incubated in 1% NP-40 lysis buffer for 30
We have demonstrated previously the potent activation of PLD by the chemokine IL-8 in T lymphocytes (18). We have now extended our findings to include the C-C chemokine RANTES in demonstrating that in the Jurkat T cell line, the activation of this enzyme occurs at subnanomolar concentrations and is dependent on the activation of small GTP-binding protein cofactors. RANTES-induced PLD activation is consistently maximal at 1 nM, a concentration corresponding to the optimal chemotaxis-inducing dose in normal T lymphocytes. Interestingly, PLD activation in T lymphocytes and Jurkat T cells appears to be an important biologic consequence of chemokine action and more readily measurable (at nanomolar concentrations) than readouts of receptor activation such as calcium flux. It was also apparent that RANTES is the only chemokine tested to date (RANTES, MIP-1α, MIP-1β, MCP-1, MCP-3, lymphotactin) that induces as robust a response as seen in this study, although the others listed were capable of low ...
Annexin A6 (AnxA6) is a Ca2+-dependent membrane-binding protein involved in vesicular traffic. The likely participation of AnxA6 in the response of lymphocytes to Ca2+ signals has not been investigated yet. The present study focuses on intracellular relocation of AnxA6 in human Jurkat T lymphoblasts upon stimulation followed by transient increase of intracellular [Ca2+] and exocytosis of interleukin-2 (IL-2). Stimulation of the cells under different experimental conditions (by lowering pH and/or by rising extracellular [Ca2+] in the presence of ionomycin) induced time-dependent transients of intracellular [Ca2+] and concomitant changes in AnxA6 intracellular localization and in IL-2 secretion, with only minor effects on cell viability and apoptosis. In resting conditions (in the presence of EGTA or with no ionophore) AnxA6 was localized uniformly in the cytosol, whereas it translocated to vesicular structures beneath the plasma membrane within 5 min following stimulation of Jurkat T cells and ...
Necroptosis is a form of regulated necrotic cell death mediated by receptor-interacting serine/threonine-protein kinase 1 (RIPK1) and RIPK3. Necroptotic cell death contributes to the pathophysiology of several disorders involving tissue damage, including myocardial infarction, stroke and ischemia-reperfusion injury. However, no inhibitors of necroptosis are currently in clinical use. Here we performed a phenotypic screen for small-molecule inhibitors of tumor necrosis factor-alpha (TNF-α)-induced necroptosis in Fas-associated protein with death domain (FADD)-deficient Jurkat cells using a representative panel of Food and Drug Administration (FDA)-approved drugs. We identified two anti-cancer agents, ponatinib and pazopanib, as submicromolar inhibitors of necroptosis. Both compounds inhibited necroptotic cell death induced by various cell death receptor ligands in human cells, while not protecting from apoptosis. Ponatinib and pazopanib abrogated phosphorylation of mixed lineage kinase domain-like
TY - JOUR. T1 - Assessment of secondary necrosis of Jurkat cells using a new microscopic system and double staining method with annexin V and propidium iodide.. AU - Honda, O.. AU - Kuroda, Masahiro. AU - Joja, I.. AU - Asaumi, Jun-Ichi. AU - Takeda, Yoshihiro. AU - Akaki, S.. AU - Togami, I.. AU - Kanazawa, Susumu. AU - Kawasaki, S.. AU - Hiraki, Y.. PY - 2000/2. Y1 - 2000/2. N2 - Using a new system developed by us for acquiring microscopic images automatically, we compared the morphological changes that apoptotic cells undergo with changes in the staining pattern of annexin V-enhanced green fluorescent protein (AV-EGFP) and propidium iodide (PI) in individual cells. Jurkat cells were treated with 5 mM CaCl2 alone, anti-Fas antibody and heating at 42 degrees C for 30 min or 46 degrees C for 60 min, and then were incubated in medium with 5 mM CaCl2. Time-lapse DNA fragmentation analysis and morphological observation revealed that the anti-Fas antibody and heating at 42 degrees C for 30 min ...
The ς binding site present in the Jurkat human T lymphocyte cell line was investigated. Jurkat cell membranes were found to have a single saturable binding site for [3H]haloperidol, a ς ligand (dissociation constant, 3.9 ± 0.3 nM). The binding of [3H]haloperidol was inhibited by several ς ligands. Northern analysis and reverse transcription-polymerase chain reaction provided evidence for the expression of the recently cloned type 1 ς-receptor (ς-R1) in Jurkat cells. The ς-R1 cDNA cloned from these cells was functional in heterologous expression systems. When expressed in mammalian cells, the cDNA-induced binding was saturable with dissociation constants of 1.9 ± 0.3 nM for [3H]haloperidol and 12 ± 2 nM for (+)-pentazocine. The binding of [3H]progesterone, a putative endogenous ligand to ς-R1, to the Jurkat cell ς-receptor could be directly demonstrated by using heterologously expressed ς-R1 cDNA. The binding of [3H]progesterone was saturable, with a dissociation constant of 88 ± 7 ...
TY - JOUR. T1 - Signaling through T lymphocyte surface proteins, TCR/CD3 and CD28, activates the HIV-1 long terminal repeat. AU - Tong-Starksen, S. E.. AU - Luciw, Paul A. AU - Peterlin, B. M.. PY - 1989. Y1 - 1989. N2 - The state of T cell activation and proliferation controls HIV-1 replication and gene expression. Previously, we demonstrated that the administration of PHA and PMA to the human T cell line Jurkat activates the HIV-1 enhancer, which is composed of two nuclear factor κB (NFκB) binding sites. Here, we show that PMA alone is sufficient for this effect. In addition, activation of T cells through the surface proteins TCR/CD3 and CD28 increased gene expression directed by the HIV-1 long terminal repeat (LTR) to the same extent as PMA. Analysis of 5 deletions in the LTR revealed that the NFκB binding sites and sequences in the upstream U3 region are required for this response. Whereas cyclosporin A did not inhibit the effect of PMA, it reduced the effects of agonists to TCR/CD3 and ...
TY - JOUR. T1 - Lysophosphatidylcholine is a regulator of tyrosine kinase activity and intracellular Ca2+ level in Jurkat T cell line. AU - Légrádi, Ádám. AU - Chitu, Violeta. AU - Szukacsov, Valéria. AU - Fajka-Boja, Roberta. AU - Szücs, Kinga Székely. AU - Monostori, Éva. PY - 2004/1/30. Y1 - 2004/1/30. N2 - Lysophospholipids, particularly lysophosphatidylcholine (lyso-PC), have been implicated in modulating T cell functions at the sites of inflammation and atherosclerosis. Although the chemotactic and immunomodulatory effects are well documented, the exact signaling pathway of lyso-PC action is poorly defined. In this work, we studied the earliest biochemical events in T cells triggered by lyso-PC. A marked and immediate tyrosine phosphorylation was induced in the leukemic T cell line, Jurkat. Phosphorylation of cellular substrates included src family kinase, p56lck and syk family kinase, ZAP70. The lyso-PC induced tyrosine phosphorylation was largely dependent on the presence of ...
Jurkat E6.1 Cell Line human from human blood(leukemic T-cell lymphoblast), 88042803; find null-null MSDS, related peer-reviewed papers, technical documents, similar products & more at Sigma-Aldrich.
Calcium release-activated calcium channels (CRAC) control influx of calcium in human T lymphocytes. Hour-long calcium elevations are necessary for efficient gene expression during T cell activation and proliferation. We report here that, the time course for store-operated Ca2+ entry is short-lived (3-4 min) and therefore, cannot account for the prolonged Ca2+ elevations necessary for NFAT translocation into nucleus. Previous findings strongly suggest that T cell activation is accompanied by cytosolic alkalinization. Here, we show that pH changes in Jurkat T cells following activation with mitogenic lectin, phytohemagglutinin (PHA), depends on the length of time of exposure and the concentration (potency) of the mitogen. For full understanding of ion fluxes involved in this process, it is important to distinguish CRAC channel subtype functions in these cells during activation as well as elucidate the pH mediated changes in Ca2+. In some experiments we show low pH with high concentrations of PHA. We also
Ca2+ release from intracellular stores is one of the major events transducing extracellular signals into living cells. Recently, a metabolite of nicotinamide adenine dinucleotide+ (NAD+), termed cyclic adenosine diphosphate-ribose (cADPr), has been described to release Ca2+ from caffeine-sensitive internal stores of cells. Jurkat T cells possess intracellular Ca2+ stores sensitive to caffeine, so a potential involvement of cADPr in Ca2+ signaling was investigated. cADPr released Ca2+ in a dose-dependent manner from intracellular stores of permeabilized Jurkat T cells. Half maximal release was obtained at 2.25 microM cADPr. Prior addition of D-myo-inositol 1,4,5-trisphosphate (Ins(1,4,5)P3) or thapsigargin did not influence cADPr-induced Ca2+ release, indicating the presence of different Ca2+ pools sensitive to Ins(1,4,5)P3 and cADPr. The specificity of the response was confirmed using the inhibitors ruthenium red, 8-NH2-cADPr, and 8-Br-cADPr. All three compounds blocked cADPr-induced, but not Ins(1,4
© 2015 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim. T lymphocytes need to detect rare cognate foreign peptides among numerous foreign and self-peptides. This discrimination seems to be based on the kinetics of TCRs binding to their peptide-MHC (pMHC) ligands, but there is little direct information on the minimum time required for processing elementary signaling events and deciding to initiate activation. Here, we used interference reflection microscopy to study the early interaction between transfected human Jurkat T cells expressing the 1G4 TCR and surfaces coated with five different pMHC ligands of 1G4. The pMHC concentration required for inducing 50% maximal IFN-γ production by T cells, and 1G4-pMHC dissociation rates measured in soluble phase or on surface-bound molecules, displayed six- to sevenfold variation among pMHCs. When T cells were dropped onto pMHC-coated surfaces, rapid spreading occurred after a 2-min lag. The initial spreading rate measured during the first 45 s, and the contact area
Our previous experiments have shown that MMTV Rem functions in nuclear export of unspliced viral RNA in rodent cells [5]. In this manuscript, we have shown that Rem functions in human cell lines. Our results also indicate that Rev and Rex can increase reporter gene expression by interaction with the MMTV RmRE in human Jurkat T cells (Figures 2 and 4). Rex also could function on the RmRE in 293T HEK cells. Prior data indicate that some retroviral export proteins function on heterologous retroviral RNAs. For example, Rex can bind and function on both the RRE and the RxRE [43, 44]. However, the interaction is not reciprocal since Rev cannot act on the RxRE [43]. In this respect, the RmRE is quite permissive since it is required for enhancement of luciferase activity by Rem, Rev and Rex in human T cells. No effect of Rem was observed on the HIV and HTLV response elements (Figure 8). Surprisingly, the Rec export protein from the human retrovirus most closely related to MMTV, HERV-K/HML, had no effect ...
HIV-1 is transcriptionally active in activated T helper (Th)-cells and inactive in naive or resting memory Th-cells. Ets-2 is a preinduction transcriptional repressor of the IL-2 gene in naive Th-cells and a candidate transcriptional repressor of HIV-1 in the same cells, because the −279 to −250 upstream region of HIV-1-LTR [repressor-activator target sequence (RATS)], that participates in HIV-1-LTR transcriptional silencing, encompasses the AAGGAG Ets-2 binding site. In this proof of concept study, we investigated whether Ets-2 represses the expression of HIV-1. To assess whether Ets-2 can repress HIV-1 transcriptional activation acting through RATS, we transfected Jurkat cells with an Ets-2 overexpression plasmid (pCDNA3-ets-2) or Ets-2 silencing plasmids (ets-2-shRNA) and, as target genes, plasmids carrying the whole HIV-1-LTR sequence (HIV-1-LTR-CAT) or two copies of the RATS sequence (2× RATS-CAT) or a point mutation in the Ets-2 binding site (2× mutantRATS-CAT) or CMV-CAT (control). Ets-2
TY - JOUR. T1 - Determination of cytokine regulated glycan expression by using molecularly imprinted polymers targeting sialic acid. AU - Shinde, Sudhirkumar. AU - El-Schich, Zahra PY - 2019/7/11. Y1 - 2019/7/11. N2 - Cancer cells often have an increased amount of glycans, such as sialic acid (SA), on the cell surface, which normallyplay an important role in cell growth, proliferation and differentiation. In this study, SA expression is determinedby fluorescent nanoprobes, molecularly imprinted polymers, SA-MIPs. The nanoprobes are synthesized with animprinting approach to produce tailor-made fluorescent core-shell particles with high affinity for cell surface SA.Inflammation and cytokine production are well known tumor promoters, modulating the cellular microenvironment,including an aberrant cell surface glycan pattern. The recombinant cytokines IL-4, IL-6, IL-8 and a cocktail ofcytokines collected from stimulated T leukemia Jurkat cells were used to induce in vitro inflammation in two ...
The mechanism through which CD28 costimulation potentiates TCR-driven gene expression is still not clearly defined. Vav-1, an exchange factor for Rho GTPases thought to regulate, mainly through Rac-1, various signaling components leading to cytokine gene expression, is tyrosine phosphorylated upon CD28 engagement. Here, we provide evidence for a key role of Vav-1 in CD28-mediated signaling. Overexpression of Vav-1 in Jurkat cells in combination with CD28 ligation strongly reduced the concentration of staphylococcus enterotoxin E/MHC required for TCR-induced NF-AT activation. Surprisingly, upon Vav-1 overexpression CD28 ligation sufficed to activate NF-AT in the absence of TCR engagement. This effect was not mediated by overexpression of ZAP-70 nor of SLP-76 but necessitated the intracellular tail of CD28, the intactness of the TCR-proximal signaling cascade, the Src-homology domain 2 (SH2) domain of Vav-1, and SLP-76 phosphorylation, an event which was favored by Vav-1 itself. Cells overexpressing Vav-1
Chang PY, Draheim K, Kelliher MA, Miyamoto S. NFKB1 is a direct target of the TAL1 oncoprotein in human T leukemia cells. Cancer Res. 2006 Jun 15; 66(12):6008-13 ...
hi! is anyone out there tried electroporating Jurkat cells? If so, can you post the electroporation conditions please. cheers venkat -- Venkat Pisupati Lab -44-01223-334131 Wellcome/CRC Institute Fax -44-01223-334134 Dept. of Genetics E.mail. vnp at mole.bio.cam.ac.uk University of Cambridge UK CB2 1QR ...
For three years, I worked at NIH, in the NICHD. I took part in the research being conducted in the lab of Dr. Brant Weinstein, working with vascular morphogenesis in embryonic zebrafish. From Jan 2008-Jan 2009, I worked with Dr. Andreas Herrlich in the Lodish lab at the Whitehead. We performed a large-scale high-throughput screen to test the effects a set of shRNAs designed to knock-down most human kinases and phophatases has on ectodomain cleavage. Specifically, we used osmotic stress as a cleavage stimulus to the EGF ligand TGF-alpha. We worked in human Jurkat cells, and mouse Baf3 cell lines. Previous 20.109 labwork [1] Currently, I work in the lab of Dr. Ed Boyden. I am involved in the Epilepsy research group, an attempt to cause and then silence seizure activity in mice using optically controlled neurons. Over the summer, I am also working with the molecular biology group, to clone multiple new mutants of rhodopsins into usable backbones, which will then be used to generate virus capable of ...
Fingerprint Dive into the research topics of Biotin requirements are lower in human Jurkat lymphoid cells but homeostatic mechanisms are similar to those of HepG2 liver cells. Together they form a unique fingerprint. ...
Anti-Cancer Properties. A team of Iranian researchers at the Vaccine and Serum Research Institute in Mashhad conducted in vitro tests to evaluate the ability of dried jujube extract to inhibit growth or induce cell death in a variety of human tumor cell lines. Scientists used the MTT colorimetric assay to measure the degree to which water extract of dried jujube reduced the proliferation of tumor cells. Although the jujube extract inhibited tumor cell proliferation in all lines, it displayed the greatest effect against Jurkat leukemia cells. In a report in the February 2008 issue of Cytotechnology, researchers said their findings confirm dried jujubes cytotoxic properties against a variety of cancer cell lines and urged further study to identify the mechanisms through which jujube works.. Anti-Inflammatory. Diabetics can benefit from jujube fruit when it is used as a medicinal rub for cuts and sores. Many diabetics suffer from poor circulation and neuropathy, which causes nerve damage, ...
One cryopreserved vial of immortalised human T lymphocyte cells stably expressing a nuclear restricted red fluorescent protein (1.5 x 106 cell/vial) Jurkat Incucyte® NucLight Red Cells are supplied as 1 mL cryopreserved vials (1.5 x 106 cells/mL in 90% FBS and 10% DMSO) containing a stable population of human T lymphoc
Plasmid NFAT/AP-1 3x luciferase from Dr. Anjana Raos lab contains the insert NFAT/AP-1 3x and is published in EMBO J. 2000 Sep 1. 19(17):4783-95. This plasmid is available through Addgene.
Thank you for your interest in spreading the word about The Journal of Immunology.. NOTE: We only request your email address so that the person you are recommending the page to knows that you wanted them to see it, and that it is not junk mail. We do not capture any email address.. ...
DOK3 Antibody 15749-1-AP has been identified with ELISA, WB, IHC. 15749-1-AP detected 53kd band in Jurkat cells with 1:500-1:1000 dilution...
NOD2 Antibody 20980-1-AP has been identified with ELISA, IF, IHC, WB. 20980-1-AP detected 100-110 kDa band in Jurkat cells with 1:500-1:1000 dilution...
WMF: please dont send me spam-mails again. Dont ever ask me again to translate articles into a language I do not speak. Especially if I have no clue what they are about (en:Cell migration, en:Subject-matter jurisdiction, en:Jurkat cells) and I am totally not interest in (en:Connie Sawyer, en:House of Kamehameha). This is not the kind of mail I left my e-mail-address for. I am interested in pigeons and poultry. If I ever felt like translating something, this would be the very good czech-pigeon-articles into de-wikipedia. ...
... lung adenocarcinoma A549 cells; human colorectal cancer cells; corneal epithelial cells; and Jurkat T-cell leukemia cells. The ... human cancer colon cells, human hepatocellular HepG2 and SMMC7721 cancer cells; mouse 3T3 cells (a fibroblast cell line); rat ... These species trigger cells to activate their death programs, i.e. apoptosis, and/or are openly toxic to the cells. 15(S)-HpETE ... The initially formed 15(S)-HpETE may be further metabolized by its parent cell or pass it to nearby cell by a process termed ...
... regulation by Ets in Jurkat T cells". J Cell Biochem. 72 (4): 492-506. doi:10.1002/(SICI)1097-4644(19990315)72:4. 3.0.CO;2-H. ... Cell. Endocrinol. 133 (2): 177-82. doi:10.1016/S0303-7207(97)00148-2. PMID 9406864. S2CID 43782586. Asa SL, Ramyar L, Murphy PR ... 2001). "The endogenous fibroblast growth factor-2 antisense gene product regulates pituitary cell growth and hormone production ... application for identifying ubiquitinated proteins in human cells". J. Proteome Res. 6 (1): 298-305. CiteSeerX ...
Némorin JG, Laporte P, Bérubé G, Duplay P (2001). "p62dok negatively regulates CD2 signaling in Jurkat cells". J. Immunol. 166 ... Cell. Biol. 23 (8): 2658-68. doi:10.1128/MCB.23.8.2658-2668.2003. PMC 152553. PMID 12665569. Master Z, Tran J, Bishnoi A, et al ... Cell. Biol. 25 (9): 3831-41. doi:10.1128/MCB.25.9.3831-3841.2005. PMC 1084282. PMID 15831486. v t e (Articles with short ... Master Z, Jones N, Tran J, Jones J, Kerbel RS, Dumont DJ (2001). "Dok-R plays a pivotal role in angiopoietin-1-dependent cell ...
"ICBP90 expression is downregulated in apoptosis-induced Jurkat cells". Annals of the New York Academy of Sciences. 1010 (1): ... in tumor cell growth". Molecular Biology of the Cell. 16 (12): 5621-9. doi:10.1091/mbc.E05-03-0194. PMC 1289407. PMID 16195352 ... Its expression peaks at late G1 phase and continues during G2 and M phases of the cell cycle. It plays a major role in the G1/S ... Cancer Cell. 25 (2): 196-209. doi:10.1016/j.ccr.2014.01.003. PMC 3951208. PMID 24486181. Ku DH, Chang CD, Koniecki J, ...
kamtschaticus has shown potent cytotoxicity against Jurkat T cells. Native Americans in the Northwest used the plant ...
Ballester A, Tobeña R, Lisbona C, Calvo V, Alemany S (Aug 1997). "Cot kinase regulation of IL-2 production in Jurkat T cells". ... The gene was identified by its oncogenic transforming activity in cells. The encoded protein is a member of the serine/ ... Molecular Cell. 11 (3): 685-94. doi:10.1016/S1097-2765(03)00070-4. PMID 12667451. Channavajhala PL, Wu L, Cuozzo JW, Hall JP, ... Nature Cell Biology. 6 (2): 97-105. doi:10.1038/ncb1086. PMID 14743216. S2CID 11683986. Belich MP, Salmerón A, Johnston LH, Ley ...
"Caspase-mediated cleavage of TRAF3 in FasL-stimulated Jurkat-T cells". Journal of Leukocyte Biology. 69 (3): 490-6. doi:10.1189 ... "The cytoplasmic domain of the lymphotoxin-beta receptor mediates cell death in HeLa cells". The Journal of Biological Chemistry ... Saha SK, Cheng G (April 2006). "TRAF3: a new regulator of type I interferons". Cell Cycle. 5 (8): 804-7. doi:10.4161/cc.5.8. ... Cell. 84 (2): 299-308. doi:10.1016/S0092-8674(00)80984-8. PMID 8565075. Hsu H, Huang J, Shu HB, Baichwal V, Goeddel DV (April ...
2007). "Cell-cycle-dependent regulation of Ca2+-activated K+ channel in Jurkat T-lymphocyte". J. Pharmacol. Sci. 104 (1): 94-8 ... 2001). "Ca2+-activated K+ channels in human leukemic Jurkat T cells. Molecular cloning, biochemical and functional ... "SK2 encodes the apamin-sensitive Ca2+-activated K+ channels in the human leukemic T cell line, Jurkat". FEBS Lett. 469 (2-3): ... 2006). "Ca2+-activated K+ channels in human melanoma cells are up-regulated by hypoxia involving hypoxia-inducible factor-1α ...
... induces apoptosis and caspase-dependent degradation of haematopoietic lineage cell-specific protein 1 (HS1) in Jurkat cells". ... Abdul-Ghani M, Megeney LA (June 2008). "Rehabilitation of a contract killer: caspase-3 directs stem cell differentiation". Cell ... Hsp60 and Hsp10 in the mitochondrial fraction of jurkat cells". The EMBO Journal. 18 (8): 2040-8. doi:10.1093/emboj/18.8.2040. ... "Caspase-mediated cleavage of TRAF3 in FasL-stimulated Jurkat-T cells". Journal of Leukocyte Biology. 69 (3): 490-6. doi:10.1189 ...
"Elongation factor-1alpha as a homologous complement activator of Jurkat cells". International Journal of Molecular Medicine. 6 ...
"Deficiency of ADAP/Fyb/SLAP-130 destabilizes SKAP55 in Jurkat T cells". The Journal of Biological Chemistry. 280 (25): 23576-83 ... Wang H, Liu H, Lu Y, Lovatt M, Wei, B, Rudd CE Functional defects of SKAP-55 deficient T-cells identify a regulatory role for ... Jo EK, Wang H, Rudd CE (June 2005). "An essential role for SKAP-55 in LFA-1 clustering on T cells that cannot be substituted by ... Cell. Biol. 2007 27, 6863-75. * Kosco KA, Cerignoli F, Williams S, Abraham RT, Mustelin T (January 2008). "SKAP55 modulates T ...
"Deficiency of ADAP/Fyb/SLAP-130 destabilizes SKAP55 in Jurkat T cells". J. Biol. Chem. 280 (25): 23576-83. doi:10.1074/jbc. ... proteins and the Arp2/3 complex link T cell receptor (TCR) signaling to the actin cytoskeleton". J. Cell Biol. 149 (1): 181-94 ... da Silva AJ, Li Z, de Vera C, Canto E, Findell P, Rudd CE (1997). "Cloning of a novel T-cell protein FYB that binds FYN and SH2 ... da Silva AJ, Janssen O, Rudd CE (1993). "T cell receptor zeta/CD3-p59fyn(T)-associated p120/130 binds to the SH2 domain of ...
Possible human expression of TMEM143 protein occurs in Jurkat cells (T lymphocyte). Organelle association puts TMEM143 in the ... This indicates the possibility of TMEM143 participation in lipid metabolic pathways and lipid cell differentiation "TMEM143 ... indicating the location of the protein inside the cell. Orthologs have been identified in more than 85 vertebrate species. No ...
Abraham, RT; Weiss, A (April 2004). "Jurkat T cells and development of the T-cell receptor signalling paradigm". Nature Reviews ... LL-100 panel cell lines cover the full spectrum of human leukemia and lymphoma including T-cell, B-cell and myeloid ... "Establishment of the T-cell large granular lymphocyte leukemia cell line MOTN-1 carrying natural killer-cell antigens". ... NCI-60, 60 human cancer cell lines used by the NCI List of breast cancer cell lines Quentmeier, H; Pommerenke, C; Dirks, WG; ...
Luo H, Wan X, Wu Y, Wu J (2001). "Cross-linking of EphB6 resulting in signal transduction and apoptosis in Jurkat cells". J. ... 2000). "T-cell-specific expression of kinase-defective Eph-family receptor protein, EphB6 in normal as well as transformed ... Eph Nomenclature Committee". Cell. 90 (3): 403-4. doi:10.1016/S0092-8674(00)80500-0. PMID 9267020. S2CID 26773768. Hock B, ... hematopoietic cells". Growth Factors. 18 (1): 63-78. doi:10.3109/08977190009003234. PMID 10831073. S2CID 9397812. Tang XX, Zhao ...
2005). "Extracellular pH modifies mitochondrial control of capacitative calcium entry in Jurkat cells". J. Biol. Chem. 280 (5 ... Discovery of the pH sensitivity of calcium entry into mammalian cells (the calcium concentration within the cell serves as a ... He specialises in various issues related to biochemistry, including bioenergetics, the role of mitochondria in cell physiology ... features which produce abnormal diffusion of metabolites within the cell as well as influence transport across the plasma ...
"Caspase-dependent activation of cyclin-dependent kinases during Fas-induced apoptosis in Jurkat cells". Proceedings of the ... "Rapid microtubule-independent dynamics of Cdc20 at kinetochores and centrosomes in mammalian cells". The Journal of Cell ... "Rapid microtubule-independent dynamics of Cdc20 at kinetochores and centrosomes in mammalian cells". The Journal of Cell ... Cell division cycle protein 27 homolog is a protein that in humans is encoded by the CDC27 gene. The protein encoded by this ...
In 2013, Runne et al., showed that PLEKHG2 is elevated in several leukemia cell lines, including Jurkat T cells. In addition, ... B cell and T cell leukemia at high frequency. In 2002, Himmel et al., used this model of acute myelogenous leukemia and showed ... by epidermal growth factor receptor signaling regulates cell morphology of Neuro-2a cells". The Journal of Biological Chemistry ... In this cell the Gβγ subunit of the trimeric G protein were interacted with PLEKHG2 directly. Ueda and colleagues also showed ...
Lindholm CK, Henriksson ML, Hallberg B, Welsh M (July 2002). "Shb links SLP-76 and Vav with the CD3 complex in Jurkat T cells ... The protein is important in hematopoiesis, playing a role in T-cell and B-cell development and activation. This particular GEF ... linker for activation of T cells) required for recruitment and activation of signalling proteins in T cells". Biochem. J. 356 ( ... Cell. Biol. 16 (1): 37-44. doi:10.1128/mcb.16.1.37. PMC 230976. PMID 8524317. Paz PE, Wang S, Clarke H, Lu X, Stokoe D, Abo A ( ...
"Increased expression of Lewis X and Y antigens on the cell surface and FUT 4 mRNA during granzyme B-induced Jurkat cell ... "Expression of cell surface Lewis X and Y antigens and FUT4 mRNA is increased in Jurkat cells undergoing apoptosis". Biochim. ... "Overexpression of fucosyltransferase IV in A431 cell line increases cell proliferation". Int. J. Biochem. Cell Biol. 39 (9): ... "Induction of FucT-VII by the Ras/MAP kinase cascade in Jurkat T cells". Blood. 102 (5): 1771-8. doi:10.1182/blood-2002-11-3551 ...
Lindholm CK, Henriksson ML, Hallberg B, Welsh M (July 2002). "Shb links SLP-76 and Vav with the CD3 complex in Jurkat T cells ... is a signal-transducing adaptor protein expressed in T cells and myeloid cells and is important in the signaling of T-cell ... SLP-76 is expressed in T-cells and related lymphocytes like natural killer cells. The amino acid sequence of the protein has a ... SLP-76 is also important in natural killer (NK) cells, in the signaling pathways of the NK cell receptors (NKRs). The SH2 ...
Lindholm CK, Henriksson ML, Hallberg B, Welsh M (Jul 2002). "Shb links SLP-76 and Vav with the CD3 complex in Jurkat T cells". ... "Expression of the Grb2-related protein of the lymphoid system in B cell subsets enhances B cell antigen receptor signaling ... GRAP2 has been shown to interact with: CCNDBP1, CD28, Linker of activated T cells, Lymphocyte cytosolic protein 2 MAP4K1, and ... Liu SK, Fang N, Koretzky GA, McGlade CJ (Jan 1999). "The hematopoietic-specific adaptor protein gads functions in T-cell ...
"Caspase-dependent activation of cyclin-dependent kinases during Fas-induced apoptosis in Jurkat cells". Proc. Natl. Acad. Sci. ... Zhao RY, Elder RT (2005). "Viral infections and cell cycle G2/M regulation". Cell Res. 15 (3): 143-9. doi:10.1038/sj.cr.7290279 ... Baldin V, Ducommun B (1995). "Subcellular localisation of human wee1 kinase is regulated during the cell cycle". J. Cell Sci. ... and G2-M cell population". Cell Growth Differ. UNITED STATES. 11 (4): 211-9. ISSN 1044-9523. PMID 10775038. Shen, M; Stukenberg ...
... cell receptor-dependent activation of the interleukin-2 gene nuclear factor for activation of T cells element in Jurkat T cells ... cell receptor-dependent activation of the interleukin-2 gene nuclear factor for activation of T cells element in Jurkat T cells ... Lindholm CK, Henriksson ML, Hallberg B, Welsh M (Jul 2002). "Shb links SLP-76 and Vav with the CD3 complex in Jurkat T cells". ... Lindholm CK, Henriksson ML, Hallberg B, Welsh M (Jul 2002). "Shb links SLP-76 and Vav with the CD3 complex in Jurkat T cells". ...
"The lymphocyte-specific tyrosine protein kinase p56lck is endocytosed in Jurkat cells stimulated via CD2". J. Immunol. 148 (12 ... is a cell adhesion molecule found on the surface of T cells and natural killer (NK) cells. It has also been called T-cell ... CD2 is a specific marker for T cells and NK cells, and can therefore be used in immunohistochemistry to identify the presence ... The great majority of T cell lymphomas and leukaemias also express CD2, making it possible to use the presence of the antigen ...
This kinase was shown to be activated rapidly during Fas-mediated apoptosis in Jurkat cells. In response to Fas receptor ... Mol Cell Biol. 24 (24): 10718-32. doi:10.1128/MCB.24.24.10718-10732.2004. PMC 533970. PMID 15572676. "Entrez Gene: FASTK Fas- ...
"Type II phosphatidylinositol 4-kinase beta associates with TCR-CD3 zeta chain in Jurkat cells". Mol. Immunol. 43 (5): 454-63. ... Cell. 23 (5): 685-95. doi:10.1016/j.molcel.2006.07.014. PMID 16949365. Srivastava R, Sinha RK, Subrahmanyam G (2006). " ...
Akao Y, Nakagawa Y, Iio A, Naoe T (2009). "Role of microRNA-143 in Fas-mediated apoptosis in human T-cell leukemia Jurkat cells ... in combination with miR-506 has shown to be effective in blocking cell cycle progression of lung cancer cell lines. Moreover, ... 145 alters smooth muscle cell maintenance and vascular homeostasis in mice: correlates with human disease". Cell Death Differ. ... mir-143 was found to be the most enriched miRNA in mouse embryonic stem cells that were differentiating into cardiac progenitor ...
... regulates cell elasticity possibly by interaction plasma membrane and cortical actin in Jurkat T-cells. MYO1G is a plasma ... Its expression is highly restricted to hematopoietic tissues and cells. It localises exclusively to the plasma membrane and is ... is a haematopoietic specific myosin that localises to the plasma membrane and regulates cell elasticity". FEBS Letters. 584 (3 ... frequency and application in hematopoietic stem cell transplantation". Clin. Chem. Lab. Med. 48 (9): 1287-93. doi:10.1515/CCLM. ...
"Stimulation of CD28 triggers an association between CD28 and phosphatidylinositol 3-kinase in Jurkat T cells". The Journal of ... Mazerolles F, Barbat C, Fischer A (September 1997). "Down-regulation of LFA-1-mediated T cell adhesion induced by the HIV ... "The involvement of the proto-oncogene p120 c-Cbl and ZAP-70 in CD2-mediated T cell activation". International Immunology. 13 (1 ... "Formation of signal transfer complexes between stem cell and platelet-derived growth factor receptors and SH2 domain proteins ...
Exposure to UV light causes increased biotinylation of histones in Jurkat cells. American Journal of Physiology - Cell ... Biotinylation of histones depends on the cell cycle in NCI-H69 small cell lung cancer cells. FASEB Journal, 19:A55 ... Stanley J. S., Griffin J. B., Zempleni J. 2001 Biotinylation of histones in human cells: effects of cell proliferation. ... Increase in histone poly(ADP-ribosylation) in mitogenactivated lymphoid cells. Experimental Cell Research, 187:77-84 ...
... prove that theory was based on the introduction of mutated form of this protein inside both TF-1 human cells and Jurkat cells, ... Apoptosis is a cell self-destruct process that removes toxic and/or useless cells during mammalian development and other life ... The cell diversity is originated by cell differentiation, which has been attributed to the activation of specific transcription ... Despite this gene being present in every cell, this protein is only expressed in different tissues and cell variety such as ...
Additionally, NADA has been observed to suppress inflammatory activation of human Jurkat T cells and to inhibit the release of ... Finally, NADA can prevent the degranulation and release of TNF from RBL- 2H3 mast cells treated with an IgE-antigen complex. ... "Inhibitory effect of N-Acyl dopamines on IgE-mediated allergic response in RBL-2H3 cells". Lipids. 48 (4): 383-393. doi:10.1007 ... NADA also promotes the inflammatory resolution of human endothelial cells activated by both endogenous (i.e. TNF) and exogenous ...
... in reticulocyte lysates and affects the expression of nuclear proteins upon stable transfection into Jurkat T-lymphoma cells". ... The protein can inhibit cell-free translation of mRNAs, suggesting that it plays a regulatory role in the translation apparatus ... Neumann F, Hemmerich P, von Mikecz A, Peter HH, Krawinkel U (January 1995). "Human ribosomal protein L7 inhibits cell-free ... Wool IG, Chan YL, Glück A (1996). "Structure and evolution of mammalian ribosomal proteins". Biochemistry and Cell Biology. 73 ...
This has been observed in Jurkat cells (primary thymocytes), MCF-7 cells, and lung epithelial cells. Release is dependent upon ... Identified in breast cancer cells, this find-me signals is released by MCF-7 cells to attract the THP-1 monocytes. Other cells ... it is best to remove dying cells before this occurs. One cell is capable of releasing multiple find-me signals. Should a cell ... There are many reasons as to why the body needs to get rid of non diseased and diseased cells. As a part of the cell's natural ...
... flow and actomyosin II arc contraction drive receptor cluster dynamics at the immunological synapse in Jurkat T cells". Mol ... F-Tractin is one of a number of F-actin probes useful in live cell imaging. Schell MJ, Erneux C, Irvine RF (2001). "Inositol 1, ... Initial studies determined that amino acids 9-52 from the rat ITPKA were useful as a live-cell reporter for actin filaments. ... Melak M, Plessner M, Grosse R (2017). "Actin visualization at a glance". J Cell Sci. 130 (3): 525-530. doi:10.1242/jcs.189068. ...
... of four members of the POU family of proteins in activated and phorbol 12-myristate 13-acetate-treated Jurkat T cells". ... retinal neurons called ganglion cells, and in cells of the B- and T-lymphocytic lineages. Brn3a was initially discovered in ... "Regulation of Hsp27 expression and cell survival by the POU transcription factor Brn3a". Cell Death and Differentiation. 11 (11 ... Cytogenetics and Cell Genetics. 74 (3): 225-6. doi:10.1159/000134422. PMID 8941380. Smith MD, Dawson SJ, Latchman DS (Jan 1997 ...
... product of the c-cbl protooncogene is the 120-kDa tyrosine-phosphorylated protein in Jurkat cells activated via the T cell ... 1995). "Reconstitution of the B cell antigen receptor signaling components in COS cells". J. Biol. Chem. 270 (45): 27072-8. doi ... Cell. Biol. 13 (9): 5877-87. doi:10.1128/MCB.13.9.5877. PMC 360336. PMID 8395016. Malek SN, Yang CH, Earnshaw WC, et al. (1996 ... Cell. Biol. 16 (9): 4735-43. doi:10.1128/MCB.16.9.4735. PMC 231474. PMID 8756631. Saouaf SJ, Wolven A, Resh MD, Bolen JB (1997 ...
Hsp60 and Hsp10 in the mitochondrial fraction of jurkat cells". EMBO J. 18 (8): 2040-8. doi:10.1093/emboj/18.8.2040. PMC ... Infection and disease are extremely stressful on the cell. When a cell is under stress, it naturally increases the production ... HSP60 has been shown to be released from specific cells like peripheral blood mononuclear cells (PBMCs) when there are ... March 2007). "Heat shock protein 60: regulatory role on innate immune cells". Cell. Mol. Life Sci. 64 (6): 742-51. doi:10.1007/ ...
... type-1 Tat protein selectively stimulates a phosphatidylinositol-specific phospholipase C nuclear pathway in the Jurkat T cell ... doi:10.1016/j.cell.2006.09.026. PMID 17081983. S2CID 7827573. Portal: Biology v t e (Articles with short description, Short ... 1991). "Human immunodeficiency virus-1 glycoproteins gp120 and gp160 specifically inhibit the CD3/T cell-antigen receptor ... 2006). "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks". Cell. 127 (3): 635-48. ...
Cells that expressed no signs of cytopathy from SFV were the Jurkat and Hut-78 T-cell lines. The phylogenetic tree analysis of ... SFV can infect a wide range of cells, with in vitro experiments confirming that fibroblasts, epithelial cells, and neural cells ... SFV causes cells to fuse with each other to form syncytia, whereby the cell becomes multi-nucleated and many vacuoles form, ... As the cell transcribes the integrated proviral genome, glycoproteins are produced and displayed at the surface of the cell. If ...
"Protein-tyrosine kinase Pyk2 is involved in interleukin-2 production by Jurkat T cells via its tyrosine 402". J. Biol. Chem. ... interacts with nephrocystin and both proteins localize to cell-cell contacts of polarized epithelial cells". Exp. Cell Res. 256 ... Fyn's normal role in cell migration and adhesion enables it to utilize the normal cell biology of integrin and FAK for cancer ... Normal integrin is a cell surface receptor that interacts with the extracellular matrix to send signals influencing cell shape ...
... expression of DHX32 in Jurkat T cells is specific and involves calcium and nuclear factor of activated T cells". Cell. Immunol ... Liu J, Yuan Y, Huan J, Shen Z (2001). "Inhibition of breast and brain cancer cell growth by BCCIPalpha, an evolutionarily ... 2007). "A role for DHX32 in regulating T-cell apoptosis". Anticancer Res. 27 (1A): 373-7. PMID 17352256. Chen Y, Alli Z, ...
... induce apoptosis and cell cycle arrest in Jurkat-T cells". BMC Complementary and Alternative Medicine. 14 (1): 65. doi:10.1186/ ... 69 Vita-Thion cell culture media and supplements Sabax Intravenous Fluids Gambro Haemodialysis Colleague Infusion Pumps Adco - ...
Hsp60 and Hsp10 in the mitochondrial fraction of jurkat cells". EMBO J. 18 (8): 2040-8. doi:10.1093/emboj/18.8.2040. PMC ... Hsp60 and Hsp10 in the mitochondrial fraction of jurkat cells". EMBO J. 18 (8): 2040-8. doi:10.1093/emboj/18.8.2040. PMC ... Cell. Cardiol. 35 (9): 1135-43. doi:10.1016/S0022-2828(03)00229-3. PMID 12967636. Shan YX, Yang TL, Mestril R, Wang PH (2003 ... Cell Mol. Genet. 24 (6): 315-26. doi:10.1023/A:1024488422990. PMID 10763410. S2CID 39860709. Richardson A, Schwager F, Landry ...
... kb cells MeSH A11. - hl-60 cells MeSH A11. - ht29 cells MeSH A11. - jurkat cells ... kb cells MeSH A11.251.860.180.465 - hl-60 cells MeSH A11.251.860.180.475 - ht29 cells MeSH A11.251.860.180.495 - jurkat cells ... cho cells MeSH A11.251.210.505 - l cells (cell line) MeSH A11.251.210.520 - llc-pk1 cells MeSH A11.251.210.700 - 3t3 cells MeSH ... l cells MeSH A11.329.228.900 - 3t3 cells MeSH A11.329.228.900.080 - balb 3t3 cells MeSH A11.329.228.900.550 - nih 3t3 cells ...
... down-regulation of gene expression by PMA and calcium ionophore in Jurkat T lymphoma cells". Biochemical and Biophysical ... December 2003). "A novel cell-cell junction system: the cortex adhaerens mosaic of lens fiber cells". Journal of Cell Science. ... These cell-cell adhesion complexes are necessary for the creation and maintenance of epithelial cell layers and barriers. As a ... "Ksp-cadherin is a functional cell-cell adhesion molecule related to LI-cadherin". Experimental Cell Research. 294 (2): 345-355 ...
... jurkat cells MeSH A15.382.490.555.567.569.500 - t-lymphocyte subsets MeSH A15.382.490.555.567.622 - lymphocytes, null MeSH ... foam cells MeSH A15.382.680.397.376 - giant cells, foreign-body MeSH A15.382.680.397.380 - giant cells, langhans MeSH A15.382. ... foam cells MeSH A15.382.812.522.376 - giant cells, foreign-body MeSH A15.382.812.522.380 - giant cells, langhans MeSH A15.382. ... killer cells MeSH A15.145.229.637.555.567.537 - killer cells, natural MeSH A15.145.229.637.555.567.537.500 - killer cells, ...
HEK 293 cells - derived from human fetal cells. Jurkat cells - a human T lymphocyte cell line isolated from a case of leukemia ... A549 cells - derived from a cancer patient lung tumor. HeLa cells - a widely used human cell line isolated from cervical cancer ... Immortalised cell lines have also found uses in biotechnology. An immortalised cell line should not be confused with stem cells ... OK cells - derived from female North American opossum kidney cells Ptk2 cells - derived from male long-nosed potoroo epithelial ...
"CD45-associated kinase activity requires lck but not T cell receptor expression in the Jurkat T cell line". The Journal of ... There are two main pools of T cells which mediate adaptive immune responses: CD4+ T cells (or helper T cells), and CD8+ T-cells ... Cytotoxic T cells are directly involved in the individuation and in the removal of infected cells, whereas helper T cells ... it is important for the activation of the T-cell receptor signaling in both naive T cells and effector T cells. The role of the ...
2002). "CCR6 colocalizes with CD18 and enhances adhesion to activated endothelial cells in CCR6-transduced Jurkat T cells". J. ... CCR6 is expressed on B-cells, immature dendritic cells (DC), T-cells (Th1, Th2, Th17, Treg), natural killer T cells (NKT cells ... 1997). "A somatic cell hybrid panel for distal 17q: GDIA1 maps to 17q25.3". Cytogenet. Cell Genet. 76 (3-4): 172-5. doi:10.1159 ... Colorectal carcinoma cells express CCR6 and CCL20. High level of CCL20 in liver chemoattract colorectal carcinoma cells and ...
The muscle cell is damaged by the depletion of ATP and possibly the high temperatures, and cellular constituents "leak" into ... Jurkat-Rott K, McCarthy T, Lehmann-Horn F (January 2000). "Genetics and pathogenesis of malignant hyperthermia". Muscle & Nerve ... Furthermore, cells expressing these channels have an increased basal cytosolic Ca2+ concentration. As these channels interact ... Cell Physiology. 287 (4): C1094-102. doi:10.1152/ajpcell.00173.2004. PMID 15201141. S2CID 18219662. Litman R, Rosenberg H; ...
... and enhances the adhesion of Jurkat cells on these cells. GRCh38: Ensembl release 89: ENSG00000172889 - Ensembl, May 2017 ... Endothelial cell lines naturally express egfl7, on the contrary to non-endothelial cells. In endothelial cells, expression is ... Cell. 125 (4): 801-14. doi:10.1016/j.cell.2006.03.032. PMID 16713569. S2CID 13709685. Parker LH, Schmidt M, Jin SW, Gray AM, ... the Egfl7 protein inhibits human aortic smooth muscle cells migration stimulated by PDGF-BB but has no effects on cell ...
Jurkat-Rott, K.; Lehmann-Horn, F. (2004). "The Patch Clamp Technique in Ion Channel Research". Current Pharmaceutical ... Preston GM, Carroll TP, Guggino WB, Agre P (1992). Appearance of water channels in Xenopus oocytes expressing red cell CHIP28 ... doi:10.3389/fphys.2010.00135 Lehmann-Horn, F.; Jurkat-Rott, K. (2003). "Nanotechnology for neuronal ion channels". Journal of ... "Properties of voltage-gated Na+ channels in the human rhabdomyosarcoma cell-line SJ-RH30: conventional and automated patch ...
These edges define a permutation matrix whose non-zero cells correspond to non-zero cells in X. ∎ There is a simple ... W. B. Jurkat and H. J. Ryser, "Term Ranks and Permanents of Nonnegative Matrices" (1967). van der Waerden, B. L. (1926), " ... and all cells are non-negative (the sum of the row sums being equal to the number of columns). Any matrix in this form can be ... λP will be a scalar multiple of a doubly stochastic matrix and will have at least one more zero cell than X. Accordingly we may ...
Cell Extract Kit for studying Cell Extracts in the research area. ... Jurkat Untreated): Total cell extracts from Jurkat cells serve ... Apoptosis Cell Extracts (Jurkat + Etoposide): Total cell extracts from Jurkat cells treated with 25 μM etoposide for 5 hours ... Jurkat Apoptosis Cell Extracts (etoposide) 2043. Toggle Between Dark and Light Modes Filter: *WB ... Western blot analysis of Jurkat Apoptosis Cell Extracts #2043 using Caspase-3 (8G10) Rabbit mAb, #9665.. Show Less Show More ...
... interacts with lck tyrosine kinase in Jurkat lymphoma cells and normal T cells. ... its function in these cells is not clearly defined. Using the Jurkat T cell lymphoma cell line and normal human T cells, we ... Upon TCR-mediated activation of Jurkat cells, MUC1 is found in the low-density membrane fractions, where linker of T cell ... MUC1 (CD227) interacts with lck tyrosine kinase in Jurkat lymphoma cells and normal T cells. ...
Pramanicin induces apoptosis in Jurkat leukemia cells: A role for JNK, p38 and caspase activation Academic Article ...
... its function in these cells is not clearly defined. Using the Jurkat T cell lymphoma cell line and normal human T cells, we ... its function in these cells is not clearly defined. Using the Jurkat T cell lymphoma cell line and normal human T cells, we ... its function in these cells is not clearly defined. Using the Jurkat T cell lymphoma cell line and normal human T cells, we ... its function in these cells is not clearly defined. Using the Jurkat T cell lymphoma cell line and normal human T cells, we ...
Retama monosperma L. (Boiss.) or Genista monosperma L. (Lam.), locally named as
深入研究「Glycogen synthase kinase-3β is critical for Interferon-α-induced serotonin uptake in human Jurkat T cells」主題。共同形成了獨特的指紋。 ... Glycogen synthase kinase-3β is critical for Interferon-α-induced serotonin uptake in human Jurkat T cells. / Tsao, Chiung Wen; ... Glycogen synthase kinase-3β is critical for Interferon-α-induced serotonin uptake in human Jurkat T cells. Journal of Cellular ... Glycogen synthase kinase-3β is critical for Interferon-α-induced serotonin uptake in human Jurkat T cells. 於:
Inhibition of PMA-induced ICAM-1 and VCAM-1 expression as well as PMA- induced adhesion of Jurkat T-cells to ECV cells by α- ... Inhibition of PMA-induced ICAM-1 and VCAM-1 expression as well as PMA- induced adhesion of Jurkat T-cells to ECV cells by α- ... Inhibition of PMA-induced ICAM-1 and VCAM-1 expression as well as PMA- induced adhesion of Jurkat T-cells to ECV cells by α- ... Inhibition of PMA-induced ICAM-1 and VCAM-1 expression as well as PMA- induced adhesion of Jurkat T-cells to ECV cells by α- ...
We investigated apoptosis and delayed luminescence (DL) of human leukemia Jurkat cells treated with the mitochondrial ... We investigated apoptosis and delayed luminescence (DL) of human leukemia Jurkat cells treated with the mitochondrial ... clonogenic survival and delayed luminescence of human leukemia jurkat T-cells. Baran I;Ganea C;Privitera S;Scordino A;BARRESI, ... Apoptosis evolved slowly, with a maximum effect observed at 48 h, when the apoptotic cell fraction was strongly correlated with ...
To approach this issue, the heterodimer and its adaptor (DAP12) were expressed in the human Jurkat leukemia T cell line; ... To approach this issue, the heterodimer and its adaptor (DAP12) were expressed in the human Jurkat leukemia T cell line; ... Moreover, reporter activation was detectable upon interaction with HLA-E+ .221-AEH cells, as well as with .221 cells incubated ... On the other hand, infection with two clinical isolates or with the endotheliotropic TB40/E strain triggered Jurkat-NKG2C+ ...
The large TFIIA subunit paralogues alphabeta and tau are largely produced in unsynchronized cell lines, yet only TFIIA ... IIA were examined during mammalian brain development and in rat embryo fibroblasts and transformed cell lines. ... Stem Cells Dev. 2006 Apr;15(2):175-90. doi: 10.1089/scd.2006.15.175. ... ATRA-treated NT2-ec cells required replating to induce a neuronal phenotype and loss of detectable TFIIA tau and gamma proteins ...
Oxidative stress induces PKR-dependent apoptosis via IFN-γ activation signaling in Jurkat T cells. / Pyo, Chul Woong; Lee, Shin ... Oxidative stress induces PKR-dependent apoptosis via IFN-γ activation signaling in Jurkat T cells. Biochemical and biophysical ... Oxidative stress induces PKR-dependent apoptosis via IFN-γ activation signaling in Jurkat T cells. In: Biochemical and ... Here, we analyzed the implication of oxidative stress in the induction of PKR-dependent apoptosis in Jurkat cells. Our results ...
Human Normal Lymphocyte Cell Line from BIOCHAIN. Cat Number: R1254148-1. UK & Europe Distribution. Order Online or Request a ... Total RNA - Human Tumor Cell Line: Jurkat , R1255815-50 , BiochainCategory: RNA / Total RNASpecies: Human Tumor Cell LineSize: ... Total RNA - Human Normal Lymphocyte Cell Line , R1254148-1 Biochain Total RNA - Human Normal Lymphocyte Cell Line , R1254148-1 ... Total RNA - Human Tumor Cell Line: Jurkat , R1255815-50 Biochain Total RNA ...
Human Jurkat Whole Cell Lysate , MBS1750982 , MybiosourceProduct Short Name: [Jurkat]Product Name Synonyme: N/AOther Names: N/ ... Rat PC12 Whole Cell Lysate , MBS1750992 , MybiosourceProduct Short Name: [PC12]Product Name Synonyme: N/AOther Names: N/ ... Human Smmc-7721 Whole Cell Lysate , MBS1750985 , MybiosourceProduct Short Name: [Smmc-7721]Product Name Synonyme: N/AOther ...
Paraquat, a herbicide linked to Parkinsons disease, generates reactive oxygen species (ROS), which causes cell death. Because ... uncovers three genes mediating PQ-induced cell death: POR is the source of PQ-mediated reactive oxygen species (ROS) generation ... we conducted a CRISPR-based positive-selection screen to identify metabolic genes essential for paraquat-induced cell death. ... required for paraquat-induced cell death. Furthermore, our results revealed POR as the source of paraquat-induced ROS ...
The catalytic subuntt of dna-dependent protein kinase is cleaved during fas-mediated apoptosis in jurkat cells. Journal of ... The catalytic subuntt of dna-dependent protein kinase is cleaved during fas-mediated apoptosis in jurkat cells. / McConnell, K ... Since apoptosis is associated with formation of dsDNA breaks, we examined in Jurkat cells the behavior of the catalytic subunit ... Since apoptosis is associated with formation of dsDNA breaks, we examined in Jurkat cells the behavior of the catalytic subunit ...
Jurkat E6.1 cells have many of the hallmarks of standard T cell transcriptional responses to activation, but lack most of the ... These data indicate that Jurkat E6.1 cells hence represent only a highly simplified model of early T cell transcriptional ... perform a basic comparison between observed transcriptional responses in Jurkat E6.1 cells and those in primary human T cells ... We present whole-transcriptome RNA-Sequencing data for Jurkat E6.1 cells in the resting state and two hours post-activation via ...
In the Jurkat cells treated with a combination of melatonin and radiation, both Annexin V-FITC(+)/PI(-) and Annexin V-FITC(+) ... The Jurkat cells were divided into four groups of control, 1 mm melatonin alone, 4 Gy irradiation-only and melatonin ... The irradiation or melatonin did not influence the JNK1 expression in Jurkat cells. The present results suggest that melatonin ... Melatonin exerts differential actions on X-ray radiation-induced apoptosis in normal mice splenocytes and Jurkat leukemia cells ...
Jurkat Cells Just-Noticeable Difference use Differential Threshold Justice Administration System Justicia adhatoda use Adhatoda ...
Inhibition of cell growth.. 2 × 105 Jurkat cells grown in presence or absence of antibody.. *p , 0.001.. Reproduced with ... Inhibition of cell growth.. 2 × 105 Jurkat cells grown in presence or absence of antibody.. *p , 0.001.. Reproduced with ... described a homophilic antibody that induced apoptosis in human tumor cells without the help of immune effector cells.[31] ...
Jurkat cells were identified as being negative for CD19 (a B cell marker, present at the cell surface of BJAB cells). ... BJAB cells were preincubated with Abs 30 minutes before addition of Jurkat cells and SEE. (. b. ) Jurkat cells were ... Jurkat cells were left unstimulated, stimulated with PMA/iono, or stimulated with BJAB cells and SEE. After 16 hours, cells ... Jurkat cells were stimulated by incubation with BJAB cells and SEE. Jurkat activation was quantified by luciferase assays 16 ...
All lanes : Jurkat cell lysate. Lysates/proteins at 15 µg per lane.. Predicted band size: 115 kDa. Observed band size: 95 kDa ... Cell lines and Lysates. Multiplex miRNA assays. Multiplex Assays. By research area. Cancer. Cardiovascular. Cell Biology. ... Cell and tissue imaging tools. Cellular and biochemical assays. Proteins and Peptides. By product type. Proteomics tools. ... Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines ...
... in Jurkat T cells. Exp Cell Res 312:374-386. CARLSSON S, CARLSSON MC, LEFFLER H (2007) Intracellular sorting of galectin-8 ... Cell nuclei were stained with Hoechst. C) Cell adhesion was quantified by counting cell nuclei in three independent experiments ... The discrepancies between the results of Yamamoto and ours are likely due to variations in sub-lines of Jurkat cells. However, ... Cell adhesion assays. Gal-8 released from GST by proteolytic treatment was used in cell adhesion assays (Cárcamo et al., 2006 ...
HIV-1 Tat upregulates BIM expression in Tat-infected Jurkat T cells. PubMed ... miR-24-3p down-regulates the expression of the apoptotic factors FasL and BIM in human natural killer cells. Title: miR-24-3p ... Melatonin induces apoptotic cell death through Bim stabilization by Sp1-mediated OTUD1 upregulation. Title: Melatonin induces ... involved_in B cell homeostasis IEA Inferred from Electronic Annotation. more info ...
Cell Lines. Assay Type. Concentration. Incubation Time. Formulation. Activity Description. PMID. human Jurkat cells. ... Cell Biology. Cell Counting Kit-8 (CCK-8). Animal Experiment. Mouse Direct PCR Kit (For Genotyping). Mouse CD3+ T-Cell ... Cell Cycle TGF-beta/Smad DNA Damage/DNA Repair Stem Cells & Wnt Hippo Ubiquitin Neuronal Signaling NF-κB GPCR & G Protein ... Stem Cell Differentiation Compound LibraryNew Stem Cell Signaling Compound Library TGF-beta/Smad compound library Traditional ...
... expressed in HEK 293 cells; find Sigma-Aldrich-ZRB1295 MSDS, related peer-reviewed papers, technical documents, similar ... Western Blotting Analysis: A 1:1,000 dilution of this antibody detected CD3d in Jurkat cell lysate.. Tested applications. ... When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell ... Flow Cytometry Analysis: 1 μg from a representative lot detected CD3d in one million Jurkat cells.. Note: Actual optimal ...
4.9. Inhibition of Intracellular ROS in a T Cell Line. Jurkat human leukemic T cell line was used for the measurement of ... in the Jurkat human leukemia cell line was higher for both powder teas compared to the same amount ... ROS intensity in Jurkat cell line added with green teas (1/500 dilution). * and # indicate differences ... ROS inhibition effect in the Jurkat human leukemia cell line was higher for both powder teas ...
Effect of glutathione depletion on caspase-3 independent apoptosis pathway induced by curcumin in Jurkat cells.. *K. Piwocka, E ... Cell cycle-specific effects of lovastatin.. *M. Jakóbisiak, S. Bruno, J. Skierski, Z. Darżynkiewicz ... Cellular quiescence induced by contact inhibition or serum withdrawal in C3H10T1/2 cells. *M. Gos, J. Miłoszewska, P. Swoboda, ... Transcriptomic signature of cell lines isolated from canine mammary adenocarcinoma metastases to lungs. *M. Król, J. Polańska, ...
... root extract induces cell death via mitochondrial-mediated caspase-dependent apoptosis in Jurkat human leukemic T cells.Jan 31 ... Fermented Arctium lappa fruit extract had an inhibitory effect on the IgE-mediated allergic response in RBL‑2H3 cells.Dec 27, ... Pubmed Data : Cell Mol Neurobiol. 2015 Apr ;35(3):335-44. Epub 2014 Oct 29. PMID: 25352420 ... Enhancement of the apoptotic effects of Arctii fructus extracts on cancer cells by the enzymatic bioconversion of lignans.Apr ...
span style=font-family:Times,serif;font-size:9pt;>The 8D3 antibody recognizes the ζ chain of the human and mouse T cell ... span style=font-family:Times,serif;font-size:9pt;>The 8D3 antibody recognizes the ζ chain of the human and mouse T cell ... Lysate from Jurkat cells was probed with anti-CD3ζ (clone 8D3, Comp. No. 51-6527GR) at concentrations of 0.5 (lane 1), 0.25 ( ... Lysate from Jurkat cells was probed with anti-CD3ζ (clone 8D3, Comp. No. 51-6527GR) at concentrations of 0.5 (lane 1), 0.25 ( ...
  • Enhances radiation-induced apoptosis in Jurkat leukemia cells, while reducing radiation-induced apoptosis in normal mice splenocytes. (aianmodena.org)
  • We have previously described that Gal-8 induces apoptosis in activated T cells interacting with certain β1 integrins and this effect is counteracted by the anti-Gal-8 autoantibodies. (scielo.cl)
  • Jurkat cells and cell lysate. (abcam.com)
  • Evaluated by Western Blotting in Jurkat cell lysate. (sigmaaldrich.com)
  • Western Blotting Analysis: A 1:1,000 dilution of this antibody detected CD3d in Jurkat cell lysate. (sigmaaldrich.com)
  • Lysate from Jurkat cells was probed with anti-CD3ζ (clone 8D3, Comp. (bdbiosciences.com)
  • Jurkat control lysate [50 µg (1 µg/µl) is provided as a Western blot positive control (Comp. (bdbiosciences.com)
  • Western blot analysis of Jurkat Apoptosis Cell Extracts #2043 using Caspase-7 Antibody, #9492. (cellsignal.com)
  • Western blot analysis of Jurkat Apoptosis Cell Extracts #2043 using PARP Antibody #9542 (left), and Cleaved PARP (Asp214) (D64E10) XP ® Rabbit mAb #5625 (right). (cellsignal.com)
  • described a homophilic antibody that induced apoptosis in human tumor cells without the help of immune effector cells. (medscape.com)
  • 2 × 10 5 Jurkat cells grown in presence or absence of antibody. (medscape.com)
  • Clone 1H2 is a ZooMAb ® rabbit recombinant monoclonal antibody that specifically detects human T-cell surface glycoprotein CD3 delta chain (CD3d). (sigmaaldrich.com)
  • The 8D3 antibody recognizes the ζ chain of the human and mouse T cell antigen receptor-associated CD3 complex. (bdbiosciences.com)
  • WB analysis of Jurkat cell lysates using GTX87071 Pyruvate Carboxylase antibody. (genetex.com)
  • K-562 cells were subjected to SDS PAGE followed by western blot with 13332-1-AP (CD94 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours. (ptglab.com)
  • An important and desirable biological activity of most antibody drugs, especially for oncology targets, is antibody-dependent cell-mediated cytotoxicity (ADCC). (genengnews.com)
  • In ADCC, circulating natural killer (NK) cells perform lytic killing of antigen-bearing target cells through specific antibody cross-linking of the two cell types. (genengnews.com)
  • Non-Ab dependent or spontaneous lysis of cells increases background and decreases the dynamic antibody-specific signal range. (genengnews.com)
  • We have exploited activation of the NFAT signaling pathway, which is intact in Jurkat cells and is activated in NK cells upon cross linking of FcγRIIIa receptor with target cell-bound specific antibody. (genengnews.com)
  • Specificity of the reporter ADCC bioassay: Serial dilutions of Rituximab (anti-CD20 chimeric monoclonal antibody drug), Trastuzumab (anti-Her2 humanized monoclonal antibody drug), or assay medium control (no antibody) were incubated for 6 hours of induction at 37°C with engineered Jurkat effector cells, with or without WIL2-S target cells, as indicated. (genengnews.com)
  • Paraquat increases cyanide-insensitive respiration in murine lung epithelial cells by activating an NAD(P)H:paraquat oxidoreductase: identification of the enzyme as thioredoxin reductase. (nature.com)
  • Burdock complex could attenuate H. pylori infection by inhibiting adhesion and subsequent inflammatory response on the gastric epithelial cells. (greenmedinfo.com)
  • Jurkat and human normal prostate epithelial cells were treated with benzo[a]pyrene to ascertain the epigenetic effects of this type of agent. (cdc.gov)
  • Benzo[a]pyrene reduced promoter methylation and increased gene expression of the same gene in Jurkat and normal prostate epithelial cells. (cdc.gov)
  • Using the Jurkat T cell lymphoma cell line and normal human T cells, we demonstrate that MUC1 is not only expressed in these cells but is also phosphorylated upon T cell receptor (TCR) ligation and associates with the Src-related T cell tyrosine kinase, p56lck. (drugbank.com)
  • The effects of clinically safe antioxidants in the regulation of adhesion molecule expression in human endothelial cells (ECV), and adherence of human Jurkat T cells to ECV cells were investigated. (elsevier.com)
  • We investigated apoptosis and delayed luminescence (DL) of human leukemia Jurkat cells treated with the mitochondrial respiration inhibitor, rotenone (ROT). (unict.it)
  • Human cytomegalovirus (HCMV) infection promotes the differentiation and persistent expansion of a mature NK cell subset, which displays high surface levels of the activating CD94/NKG2C NK cell receptor, together with additional distinctive phenotypic and functional features. (frontiersin.org)
  • By contrast, activation of Jurkat-NKG2C+ was undetectable upon interaction with Human Fetal Foreskin Fibroblasts (HFFF) infected with HCMV laboratory strains (i.e. (frontiersin.org)
  • This function is mainly fulfilled by members of the human killer-cell immunoglobulin-like receptor (KIR) family, which recognize sets of classical HLA class I (HLA-I) molecules, and by the CD94/NKG2A lectin-like heterodimer specific for HLA-E. Conversely, other KIRs and CD94/NKG2C, which display a lower affinity for HLA-I ligands trigger protein tyrosine kinase pathways through DAP12, an adaptor with immunoreceptor tyrosine-based activation motifs. (frontiersin.org)
  • In this regard, human cytomegalovirus (HCMV) infection has been shown to promote the differentiation and persistent expansion of a mature NK cell subset, which displays high surface levels of the activating CD94/NKG2C NKR (NKG2C bright ), together with additional distinctive phenotypic and functional features ( 7 - 12 ). (frontiersin.org)
  • Steady-state protein levels of the TFIIA tau, alphabeta, and gamma subunits were significantly reduced when human embryonal (ec) and hepatic carcinoma cell lines were stimulated to differentiate with either all-trans-retinoic acid (ATRA) or sodium butyrate. (nih.gov)
  • We compare early transcriptional responses in the presence and absence of the chemokines CXCL12 and CCL19, and perform a basic comparison between observed transcriptional responses in Jurkat E6.1 cells and those in primary human T cells using publicly deposited data. (ox.ac.uk)
  • 1998. Protection of human upper respiratory tract cell lines against sulphur mustard toxicity by hexamethylenetetramine (HMT). (cdc.gov)
  • KLH-conjugated linear peptide corresponding to 15 amino acids from the extracellular domain of human T-cell surface glycoprotein CD3 delta chain (CD3d). (sigmaaldrich.com)
  • Tetrabromobenzotriazole (TBBt) and tetrabromobenzimidazole (TBBz) as selective inhibitors of protein kinase CK2: evaluation of their effects on cells and different molecular forms of human CK2. (semanticscholar.org)
  • Cytostatic and cytotoxic activity of synthetic genistein glycosides against human cancer cell lines. (semanticscholar.org)
  • Our aim was to examine the involvement of G1 cell-cycle regulators in cell growth dysregulation induced by HTLV-I. Compared to uninfected cells, higher expression levels of cyclin D1 and D2 mRNA were detected in HTLV-I-infected T-cell lines, which were at least in part mediated by the viral transforming protein Tax since Tax activated both cyclin D1 and D2 promoters in the human T-cell line Jurkat. (mdc-berlin.de)
  • Our data suggest that induction of cyclins D1 and D2 by Tax is involved in IL-2-independent cell-cycle progression as well as IL-2-independent transformation of primary human T cells by HTLV-I. High expression levels of cyclin D1 and D2 mRNAs were also detected in some patients with ATL. (mdc-berlin.de)
  • On the other hand, human leukemic Jurkat cells deficient in Bax showed dramatically reduced apoptosis in response to As 2 O 3 . (elsevier.com)
  • Human immunodeficiency virus (HIV) is the causative agent of the deadly disease AIDS, which is characterized by the progressive decline of CD4 + T-cells. (microbiologyresearch.org)
  • Human immunodeficiency virus induces a dual regulation of Bcl-2, resulting in persistent infection of CD4 + T- or monocytic cell lines. (microbiologyresearch.org)
  • It has been previously found that hTERT activity is down-regulated by the human T cell leukaemia virus type 1 (HTLV-1) Tax protein in HTLV-1 transformed T lymphocytes. (biomedcentral.com)
  • In human cells, telomere length is maintained by telomerase (hTERT), a human reverse transcriptase that adds TTAGGG repeats onto the 3' ends of telomeres [ 4 ]. (biomedcentral.com)
  • Recently, involvement of cell cycle proteins was demonstrated for phorbol myristate acetate (PMA)-triggered human PMN-derived NETs. (bvsalud.org)
  • Jurkat, human T-Iymphocyte cells were exposed to 1763 MHz RF radiation at an average specific absorption rate (SAR) of 10 W/kg for one hour and harvested immediately (R0) or after five hours (R5). (bvsalud.org)
  • 2012) reported on human skin penetration of cobalt nanoparticles through intact and damaged skin suggesting that Co applied as NPs is able to penetrate the human skin in an in-vitro diffusion cell system. (malvernpanalytical.com)
  • The hypothesis that MHC-specific activating NKR may contribute to the innate response against pathogens was supported by the evidence that Ly49H specifically interacts with the MHC class I-related murine cytomegalovirus glycoprotein m157, triggering NK cell effector functions and the development of a memory-like response that confers resistance against the viral infection in some mice strains ( 3 - 5 ). (frontiersin.org)
  • Several signaling pathways are activated in the NK effector cells as a result of multiple FcγRIIIa receptors being engaged by the target cell-bound antibodies. (genengnews.com)
  • These lead to lysis of target cells and induction of specific cytokines (e.g., through NFAT, or nuclear factor activator of T-cells) in the effector cells. (genengnews.com)
  • Extra controls are required to subtract spontaneous lysis from both effector and target cells. (genengnews.com)
  • Promega's reporter-based ADCC bioassay is a new cell-based genetic reporter assay that uses an engineered Jurkat cell line as the effector cell population, avoiding the requirement of purified NK cells from blood donors and avoiding the variability in effector function. (genengnews.com)
  • We also engineered the same Jurkat cell line to co-express the FcγRIIIa receptor (high affinity variant), thus completing the engineering needed to generate an effector cell line able to quantify Fc effector function of therapeutic antibodies in ADCC. (genengnews.com)
  • In our ADCC reporter bioassay, effector cells are essentially assay reagents. (genengnews.com)
  • The Jurkat effector cells are provided in frozen, thaw-and-use format. (genengnews.com)
  • Paraquat, a herbicide linked to Parkinson's disease, generates reactive oxygen species (ROS), which causes cell death. (nature.com)
  • Recently, melatonin was shown to possess proapoptotic action by increasing reactive oxygen species in certain cancer cells. (aianmodena.org)
  • Flow cytometry was used for cell cycle, apoptosis, and reactive oxygen species (ROS) accumulation analysis. (aacrjournals.org)
  • SMEZ expression was also analyzed by using the more sensitive Jurkat cell assay, which has a threshold of approximately 10 pg/mL. (cdc.gov)
  • c Jurkat assay. (cdc.gov)
  • With the improved assay design and procedure, and cells as reagents, the new reporter-based bioassay format outperforms classic ADCC in many key parameters: low variability, improved accuracy and precision, ease of assay procedure, and low background (sensitivity). (genengnews.com)
  • IMSEAR at SEARO: Drug sensitivity assay for leukaemic cells by flow cytometry. (who.int)
  • Gupta M, Naik S, Pandey CM, Dabadghao S. Drug sensitivity assay for leukaemic cells by flow cytometry. (who.int)
  • Here we report a simplified flow cytometry based assay for evaluating the in vitro drug sensitivity of leukaemic cells. (who.int)
  • For comparison MTT assay was also performed using prednisolone on Jurkat and daunorubicin on HL-60. (who.int)
  • Mean LD50 of prednisolone for Jurkat cells by flow cytometry was 0.805 +/- 0.058 mg/ml and by MTT assay 0.866 +/- 0.115 mg/ml. (who.int)
  • The inter-assay variation for the LD50 by flow cytometry based assay was found to be 6, 14 and 10 per cent for Jurkat, HL-60 and K 562 respectively. (who.int)
  • INTERPRETATION & CONCLUSION: We report a flow cytometry based drug-sensitivity assay for leukaemic cells, which uses a single dye staining and is rapid, technically simple and reproducible. (who.int)
  • The modification of radiation-induced apoptosis by melatonin and the expression of apoptosis-associated upstream regulators were studied in normal mice splenocytes and Jurkat T leukemia cells. (aianmodena.org)
  • 5-Aza-2′-deoxycytidine (DAC) induced cell-cycle arrest and apoptosis in leukemia cells. (aacrjournals.org)
  • DAC induced delayed ROS accumulation in leukemia cells but not in solid tumor cells and p53 expression was dispensable for ROS increase. (aacrjournals.org)
  • Thymoquinone Inhibits Growth of Acute Myeloid Leukemia Cells through Reversal SHP-1 and SOCS-3 Hypermethylation: In Vitro and In Silico Evaluation. (cdc.gov)
  • Western blot analysis of Jurkat Apoptosis Cell Extracts #2043 using Caspase-3 (8G10) Rabbit mAb, #9665. (cellsignal.com)
  • Western Blot of MCF-7, PC3, Jurkat, and A375 cell lysates using RM228, showed a band of Her2 expressed only in MCF-7 cells. (revmab.com)
  • Similar inhibitory and activating NK cell receptors (NKR) have been identified among the murine Ly49 and NKG2 lectin-like receptor families ( 1 , 2 ). (frontiersin.org)
  • The leukaemia-derived Jurkat E6.1 cell line has been used as a model T cell in the study of many aspects of T cell biology, most notably activation in response to T cell receptor (TCR) engagement. (ox.ac.uk)
  • The T-cell receptor (TCR), expressed by thymus-derived lymphocytes, is a multi-component complex responsible for recognizing antigen in the context of MHC molecules. (bdbiosciences.com)
  • 2012). Cellular processes that can be modulated by galectin cell surface interactions include cell adhesion, spreading, migration, proliferation, differentiation and apoptosis (Dennis et al. (scielo.cl)
  • Aim: Leukemia is characterized by uncontrolled marrow cell proliferation and metastatic foci. (drrathresearch.org)
  • In acute leukemias PI3K/Akt signaling activity was demonstrated to be correlated with an inferior prognosis via contribution to proliferation, survival and drug resistance in acute myeloid leukemia 3,10-12 , in T-cell acute lymphoblastic leukemia (T-ALL) 13 and B-cell precursor acute lymphoblastic leukemia (BCP-ALL). (researchsquare.com)
  • Our group was the first to characterize ion channels in lymphocytes and the first to demonstrate a specific requirement for ion channel activity in T cell functions including: membrane potential, cell volume, calcium signaling, cellular motility, gene expression, and cell proliferation. (uci.edu)
  • When transfecting with high levels of hY3 mimic molecules, cell proliferation rate decreased while IL13 mRNA levels increased upon stimulation compared to stimulated control cells. (bvsalud.org)
  • Our results show the effect of increased hY3 levels on cell proliferation and the levels of IL13 mRNA in Jurkat cells. (bvsalud.org)
  • Upon TCR-mediated activation of Jurkat cells, MUC1 is found in the low-density membrane fractions, where linker of T cell activation is contained. (drugbank.com)
  • CD94, also known as KLRD1, is a membrane protein that is preferentially expressed on NK cells. (ptglab.com)
  • Oxidative stress induced by engineered NP is due to acellular factors such as particle surface, size, composition, and presence of metals, while cellular responses such as mitochondrial respiration, NP-cell interaction, and immune cell activation are responsible for ROS-mediated damage. (cdc.gov)
  • Different effects of staurosporine, an inhibitor of protein kinases, on the cell cycle and chromatin structure of normal and leukemic lymphocytes. (semanticscholar.org)
  • The data indicate that the kinase(s) involved in the regulation of cell exit from G1 and G2, respectively, in normal and leukemic lymphocytes may have different sensitivities to staurosporine, which suggests that the mechanisms controlling exit fromG1 in these cells may be different. (semanticscholar.org)
  • Effect of glutathione depletion on caspase-3 independent apoptosis pathway induced by curcumin in Jurkat cells. (semanticscholar.org)
  • These data suggest that As 2 O 3 might exert the cell killing in part by inducing Bax activation through a Bcl-2-suppressible pathway in hematopoietic cells that is caspase independent and intracellular ROS regulated. (elsevier.com)
  • In particular, the study, which is detailed in a paper published in the current edition of Cell , found that caspase cleavage can expose new sites for phosphorylation and that phosphorylation, which has typically been thought to protect proteins from cleavage, can, in fact, promote caspase proteolysis. (genomeweb.com)
  • The researchers used the new system to again investigate the intrinsic apoptotic pathway, looking to better understand how caspase protealysis and phosphorylation interact during cell death. (genomeweb.com)
  • Studying Jurkat T-cells, they identified more than 500 apoptosis-specific phosphorylation events, showing they were enriched on cleaved proteins and around sites of caspase cleavage. (genomeweb.com)
  • To further elucidate the involvement of caspases in neuronal cell death induced by focal stroke we developed a panel of antibodies and investigated the spatial and temporal pattern of both caspase-8 and caspase-3 expression. (jneurosci.org)
  • These data indicate that the pattern of caspase expression occurring during delayed neuronal cell death after focal stroke will vary depending on the neuronal phenotype. (jneurosci.org)
  • We investigated in vitro and in vivo synergistic effects of a nutrient mixture (NM) containing ascorbic acid, lysine, proline, and green tea extract, on acute promyelocytic leukemia HL-60 cells. (drrathresearch.org)
  • Redundant and non-redundant functions of different galectins are accomplished based on the glycan selectivity of their CRDs, subsets of cell surface receptors they recognize and consequential modulation of the corresponding signaling networks (Bi et al. (scielo.cl)
  • Several previous studies have reported the role of HIV-encoded proteins in apoptosis modulation, but the molecular basis for apoptosis evasion of some chronically HIV-infected cells and reactivated latently HIV-infected cells still needs to be elucidated. (microbiologyresearch.org)
  • The current review summarizes our present understanding of apoptosis modulation in HIV-infected cells, uninfected bystander cells and latently infected cells, with a focus on highlighting strategies to activate the apoptotic pathway to kill latently infected cells. (microbiologyresearch.org)
  • Ionizing Radiation Induces Morphological Changes and Immunological Modulation of Jurkat Cells. (tu-darmstadt.de)
  • Our findings link HTLV-I infection to changes in cellular D-type cyclin gene expression, transformation of T cells and subsequent development of T-cell leukemia. (mdc-berlin.de)
  • The phosphatidylinositol 3-kinase (PI3K) / Akt / mammalian target of rapamycin (mTOR) signaling axis plays an important physiologic role in protein synthesis, gene transcription, cell growth and apoptosis. (researchsquare.com)
  • Finally, ectopic expression of hTERT in one HTLV-1 T cell line induces a marked decrease in the transcription of the POT1 gene. (biomedcentral.com)
  • Inhibits IL-2 gene expression (IC 50 = 73 nM) in Jurkat cells and shows immunosuppressive activity in a mouse model. (emdmillipore.com)
  • Treatment of ECV cells with PMA induced generation of intracellular oxidants. (elsevier.com)
  • In cell adhesion assays, Gal-8 precluded the interaction of LFA-1 with its ligand Intracellular Adhesion Molecule-1 (ICAM-1). (scielo.cl)
  • Intracellular uptake of reporter vector encoded with EGFP at 24 hr following transfection of Jurkat cells with Neon Transfection System. (thermofisher.com)
  • Blocks cell cycle progression at the G1 phase in HeLa cells and induces a 12-fold increase in intracellular levels of gelsolin. (emdmillipore.com)
  • Apoptosis Cell Extracts (Jurkat + Etoposide): Total cell extracts from Jurkat cells treated with 25 μM etoposide for 5 hours serve as a positive control for activated apoptotic cascades. (cellsignal.com)
  • Apoptosis evolved slowly, with a maximum effect observed at 48 h, when the apoptotic cell fraction was strongly correlated with clonogenic survival. (unict.it)
  • Finally, siRNA-mediated depletion of IFN-γ or pkr efficiently downregulated H 2 O 2 -mediated apoptotic cell death. (elsevier.com)
  • Depending on the stage of the virus life cycle and host cell type, these viral proteins act as mediators of pro- or anti-apoptotic signals. (microbiologyresearch.org)
  • Dix, Simon, and Cravatt introduced the original PROTOMAP system in a 2008 Cell paper in which they described how they used it to generate a proteome-wide profile of proteolytic events induced by the intrinsic apoptotic pathway. (genomeweb.com)
  • And so it's likely that by manipulating phosphorylation you might be able to alter the response of cells to apoptotic stimuli. (genomeweb.com)
  • an erythroleukaemic cell line, K 562 and a myelomonocytic cell line HL-60) was investigated by flow cytometry. (who.int)
  • THP-1 cell line monocytes were differentiated into MQs by phorbol 12-myristate 13-acetate (PMA). (bvsalud.org)
  • We investigated the potential role of RNY3 RNA (hY3) in the TH2 cell inflammatory response using the Jurkat cell line as a model. (bvsalud.org)
  • ATRA-treated NT2-ec cells required replating to induce a neuronal phenotype and loss of detectable TFIIA tau and gamma proteins. (nih.gov)
  • MUC1 (CD227) interacts with lck tyrosine kinase in Jurkat lymphoma cells and normal T cells. (drugbank.com)
  • b ) Jurkat cells were transfected with NFAT, AP-1, or NF-κB reporter plasmid and stimulated as in a . (jci.org)
  • Inhibitory receptors specific for MHC class I molecules with immunoreceptor tyrosine-based inhibitory motifs play a key role in preventing NK cell responses against normal autologous cells. (frontiersin.org)
  • Jurkat E6.1 cells have many of the hallmarks of standard T cell transcriptional responses to activation, but lack most of the depth of responses in primary cells. (ox.ac.uk)
  • These data indicate that Jurkat E6.1 cells hence represent only a highly simplified model of early T cell transcriptional responses. (ox.ac.uk)
  • We propose that its expression reduces T cell responses to KSHV-infected B cells early in infection, thereby diminishing antiviral cytokine release and the production of stimulatory signals for CTL generation. (jci.org)
  • NP-induced oxidative stress responses are torch bearers for further pathophysiological effects including genotoxicity, inflammation, and fibrosis as demonstrated by activation of associated cell signaling pathways. (cdc.gov)
  • Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected CD3d in Hut78 and MOLT-4 cell lysates. (sigmaaldrich.com)
  • Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected CD3d in Jurkat cells. (sigmaaldrich.com)
  • Abrogation of MUC1 expression in Jurkat cells by MUC1-specific small interfering RNA resulted in defects in TCR-mediated downstream signaling events associated with T cell activation. (drugbank.com)
  • Our previous studies have found that interferon-alpha (IFN-α) up-regulates serotonin transporter (5-HTT) expression and induces 5-HT uptake in T cells. (elsevier.com)
  • The thiol antioxidant, α- lipoate, at clinically relevant doses down-regulated phorbol 12-myristate 13- acetate (PMA)-induced adhesion molecule expression and cell-cell adhesion. (elsevier.com)
  • Inhibition of PMA-induced ICAM-1 and VCAM-1 expression as well as PMA- induced adhesion of Jurkat T-cells to ECV cells by α-lipoate was dose dependent (50-250 μM). (elsevier.com)
  • 01) expression in cells pretreated with 100 μM α-lipoate compared to PMA-activated untreated cells. (elsevier.com)
  • Inhibition of PMA-induced adhesion molecule expression and cell-cell adhesion was more pronounced when a combination of antioxidants, α-lipoate and α-tocopherol, were used compared to the use of either of these antioxidant alone. (elsevier.com)
  • The treatment of cells with the specific JAK-STAT inhibitor, AG490, reduced the PKR expression, and suppressed PKR-dependent cell death. (elsevier.com)
  • These results indicated that oxidative stress induces PKR expression essentially via the IFN-γ activation signal, and causes apoptosis in Jurkat T cells. (elsevier.com)
  • The irradiation or melatonin did not influence the JNK1 expression in Jurkat cells. (aianmodena.org)
  • Histograms represent CD69 expression on Jurkat cells. (jci.org)
  • Mechanism of activation-induced cell death of T cells and regulation of FasL expression. (microbiologyresearch.org)
  • We evaluated the effect of hY3 over cell stimulation and the expression of the TH2 cytokine IL13. (bvsalud.org)
  • For instance, CNT-induced oxidative stress triggers cell signaling pathways resulting in increased expression of proinflammatory and fibrotic cytokines [ 12 ]. (cdc.gov)
  • This is the first report describing the transcriptomic signature of canine cancer metastatic cells and suggests that this pathway may be essential for mammary cancer cells to have a metastatic potential. (semanticscholar.org)
  • However, 5-azanucleosides exerted long-lasting effects, reducing cell viability, changing cell morphology, and affecting phosphoinositide 3-kinase (PI3-kinase)/Akt signaling pathway. (biomedcentral.com)
  • a ) Twenty-four hours after transfection with a RE/AP reporter plasmid, Jurkat cells were stimulated by incubation with BJAB cells and SEE. (jci.org)
  • The combined effects of these two drug classes on cell viability, apoptosis, signaling pathways, and colony formation were investigated. (biomedcentral.com)
  • HIV-1-encoded proteins such as envelope gp120 (glycoprotein gp120), Tat (trans-activator of transcription), Nef (negative regulatory factor), Vpr (viral protein R), Vpu (viral protein unique) and protease are known to be effective in modulating host cell signalling pathways that lead to an alteration in apoptosis of both HIV-infected and uninfected bystander cells. (microbiologyresearch.org)
  • Altering cell death pathways as an approach to cure HIV infection. (microbiologyresearch.org)
  • associated signal transduction pathways and cell biological coupling mechanisms. (stanford.edu)
  • However, the specific cell death pathways and key upstream sensors activated in the context of Candida and Aspergillus infections are unknown. (jbc.org)
  • A role of Gal-8 in the immune system has been proposed based on its effects in immune cells, including T and B lymphocytes, as well as the presence of anti-Gal-8 autoantibodies in the prototypic autoimmune disease systemic lupus erythematosus (SLE). (scielo.cl)
  • Tanshinone I, an active principle isolated from Salvia miltiorrhiza (Danshen), is structurally similar to tanshinone IIA and may possess similar cytotoxic effects on tumor cells. (selleckchem.com)
  • Erastin is a ferroptosis activator by acting on mitochondrial VDAC, exhibiting selectivity for tumor cells bearing oncogenic RAS. (selleckchem.com)
  • Flow cytometry was used to determine LD50 (50% inhibitory concentration) for prednisolone on Jurkat and daunorubicin on HL 60 and K 562 cell lines respectively. (who.int)
  • Jurkat activation was quantified by luciferase assays 16 hours after stimulation. (jci.org)
  • The Neon Transfection System offers an innovative electroporation transfection method that utilizes a proprietary biologically compatible pipette tip chamber to generate a more uniform electric field for a significant increase in transfection efficiency and cell viability. (thermofisher.com)
  • Over 140 cell types have been successfully transfected with the Neon Transfection System. (thermofisher.com)
  • High transfection efficiency of Jurkat cells with Neon Transfection System. (thermofisher.com)
  • We have simplified your work with just one transfection kit that is compatible with all cell types. (thermofisher.com)
  • Start your transfection with our optimized protocols for popular cell types, or follow our standard simple optimization procedure for all new cell types. (thermofisher.com)
  • The result is less toxicity to the cells and higher transfection efficiencies. (thermofisher.com)
  • Access cell culture & transfection application resources for more success as you plan and execute your experiments. (thermofisher.com)
  • Find tips, troubleshooting help, and resources for your cell culture & transfection workflows. (thermofisher.com)
  • In Jurkat cells, hY3 levels increased upon stimulation with phorbol 12-myristate 13-acetate (PMA) and ionomycin. (bvsalud.org)
  • Emerging data indicate that 5-azanucleosides are able to sensitize cancer cells to the standard chemotherapeutic agents and contribute to overcoming intrinsic or acquired chemoresistance. (biomedcentral.com)
  • We conclude that TFIIA tau is associated with undifferentiated cells during development, yet is down-regulated at the chromatin level upon cellular differentiation. (nih.gov)
  • Consequently, it is necessary to investigate so-called off-target effects and their impact on cell survival and differentiation. (aacrjournals.org)
  • Inhibition of cell growth. (medscape.com)
  • Some of the paradigms for NP-mediated toxicity include oxidative stress, inflammation, genetic damage, and the inhibition of cell division and cell death [ 8 - 11 ]. (cdc.gov)
  • These results suggest that Gal-8 can exert immunosuppressive action not only by inducing apoptosis in activated T cells but also by negatively modulating the crucial function of LFA-1 in the immune system, while function-blocking autoantibodies counteract these effects. (scielo.cl)
  • We continue to develop and apply single-cell approaches to investigate the immune system. (uci.edu)
  • Innate immune-mediated programmed cell death (pyroptosis, apoptosis, necroptosis) is an integral part of host defense against pathogens. (jbc.org)
  • M1/M2 polarization of immune cells including microglia has been well characterized. (bvsalud.org)
  • The DNA-dependent protein kinase (DNA-PK) is a serine/threonine kinase linked to DNA repair, cell cycle progression and V(D)J recombination. (elsevier.com)
  • McConnell, KR & Hardin, JA 1996, ' The catalytic subuntt of dna-dependent protein kinase is cleaved during fas-mediated apoptosis in jurkat cells ', Journal of Investigative Medicine , vol. 44, no. 3, pp. 236a. (elsevier.com)
  • The research was performed using a modified version of the Protein Topography and Migration Analysis Platform, or PROTOMAP, system, a technology developed several years ago in the lab of Benjamin Cravatt, a Scripps professor and leader of the Cell study. (genomeweb.com)
  • Load 10 µl of untreated and etoposide treated Jurkat Apoptosis Control Cell Extracts (etoposide) per lane. (cellsignal.com)
  • Here, we demonstrate that pretreatment with DNA demethylating agents, 5-aza-2′-deoxycytidine and 5-azacytidine, sensitizes CRC cells to topoisomerase inhibitors (irinotecan, etoposide, doxorubicin, mitoxantrone), reducing cell viability and clonogenicity and increasing programmed cell death more effectively than individual compounds at the same or even higher concentrations. (biomedcentral.com)
  • The combinatorial, but not separate, treatment with low doses of 5-aza-2′-deoxycytidine (0.1 μM) and etoposide (0.5 μM) caused a long-lasting (almost 70 days) reduction in clonogenic/replating ability of DLD-1 cells. (biomedcentral.com)
  • 50-Hertz magnetic field and calcium transients in Jurkat cells: results of a research and public information dissemination (RAPID) program study. (cdc.gov)
  • Cancer cells are characterized by genome-wide hypomethylation compared to normal cells leading to chromatin architecture reorganization, genomic instability, loss of imprinting, and activation of oncogenes. (biomedcentral.com)
  • Western blotting is an important technique used in cell and molecular biology. (delos.info)
  • The results are discussed in the framework of previous observations supporting the hypothetical existence of specific ligand(s) for CD94/NKG2C in HCMV-infected cells. (frontiersin.org)
  • As a consequence, loss of telomeric DNA results in replicative senescence through chromosome damage and decrease in cell viability [ 5 ]. (biomedcentral.com)
  • Ample cell banks enable consistent supply and consistent results. (genengnews.com)
  • over 140 cell lines were tested with optimized ready-to-use conditions, efficiency and viability. (thermofisher.com)
  • 5-Azanucleosides did not cause considerable immediate toxic effects as evaluated by analysis of cell viability, apoptosis, DNA damage (γH2A.X), and endoplasmic reticulum (ER) stress (CHOP). (biomedcentral.com)
  • Flow Cytometry Analysis: 1 μg from a representative lot detected CD3d in one million Jurkat cells. (sigmaaldrich.com)
  • Avoid the hassle of determining which proprietary buffer kit will work with your favorite cell type. (thermofisher.com)
  • The large TFIIA subunit paralogues alphabeta and tau are largely produced in unsynchronized cell lines, yet only TFIIA alphabeta is observed in a number of differentiated tissue extracts. (nih.gov)
  • Since apoptosis is associated with formation of dsDNA breaks, we examined in Jurkat cells the behavior of the catalytic subunit during Fas (Apo-1, CD95) mediated apoptosis. (elsevier.com)
  • Oxidants are known to be involved in the regulation of cell adhesion processes. (elsevier.com)
  • Apoptosis is a regulated physiological process leading to cell death. (cellsignal.com)
  • Because the source of paraquat-induced ROS production remains unknown, we conducted a CRISPR-based positive-selection screen to identify metabolic genes essential for paraquat-induced cell death. (nature.com)
  • Our screen uncovered three genes, POR (cytochrome P450 oxidoreductase), ATP7A (copper transporter), and SLC45A4 (sucrose transporter), required for paraquat-induced cell death. (nature.com)
  • Figure 1: POR, ATP7A, and SLC45A4 are essential for paraquat-induced cell death. (nature.com)
  • Figure 3: Mitochondrial complex I is not necessary for paraquat-induced cell death. (nature.com)
  • We surmise that cleavage of DNA-PK cs may represent a mechanism for regulating the function of DNA-PK during programmed cell death. (elsevier.com)
  • To cure HIV infections completely, it is crucial to reactivate latent HIV from cellular pools and to drive these apoptosis-resistant cells towards death. (microbiologyresearch.org)
  • A number of studies have provided evidence that neuronal cell loss after stroke involves programmed cell death or apoptosis. (jneurosci.org)
  • These distinct changes in cellular structure differentiate apoptosis from necrosis, an alternative form of cell death. (jneurosci.org)
  • Per cent cell death could directly be assessed on a flow cytometer by measuring the fluorescence after staining with propidium iodide (PI). (who.int)
  • Here, we analyzed the implication of oxidative stress in the induction of PKR-dependent apoptosis in Jurkat cells. (elsevier.com)
  • Pyo, CW, Lee, SH & Choi, SY 2008, ' Oxidative stress induces PKR-dependent apoptosis via IFN-γ activation signaling in Jurkat T cells ', Biochemical and biophysical research communications , vol. 377, no. 3, pp. 1001-1006. (elsevier.com)
  • Head and neck squamous cell carcinoma and myeloid leukemia are the major causes of cancer related morbidity and mortality in Fanconi anemia patients. (drrathresearch.org)
  • BACKGROUND & OBJECTIVES: Drug sensitivity assays are useful in oncology practice for evaluating the sensitivity of malignant cells to anti-cancer drugs. (who.int)
  • Evidence for the cure of HIV infection by CCR5Δ32/Δ32 stem cell transplantation. (microbiologyresearch.org)
  • Stem cell transplantation in the context of HIV-how can we cure HIV infection? (microbiologyresearch.org)
  • hTERT is normally expressed in stem cells and in germ cells, but is present at much reduced levels in many adult somatic cells. (biomedcentral.com)
  • The mechanisms underlying the development of adaptive NK cells remain uncertain but some observations support the involvement of a cognate interaction of CD94/NKG2C with ligand(s) displayed by HCMV-infected cells. (frontiersin.org)
  • Induces reversion of oncogenic ras-tranformed NIH 3T3 cells to a normal morphology. (emdmillipore.com)