Atomic species differing in mass number but having the same atomic number. (Grant & Hackh's Chemical Dictionary, 5th ed)
Techniques for labeling a substance with a stable or radioactive isotope. It is not used for articles involving labeled substances unless the methods of labeling are substantively discussed. Tracers that may be labeled include chemical substances, cells, or microorganisms.
Spectroscopic method of measuring the magnetic moment of elementary particles such as atomic nuclei, protons or electrons. It is employed in clinical applications such as NMR Tomography (MAGNETIC RESONANCE IMAGING).
Stable nitrogen atoms that have the same atomic number as the element nitrogen, but differ in atomic weight. N-15 is a stable nitrogen isotope.
Stable carbon atoms that have the same atomic number as the element carbon, but differ in atomic weight. C-13 is a stable carbon isotope.
NMR spectroscopy on small- to medium-size biological macromolecules. This is often used for structural investigation of proteins and nucleic acids, and often involves more than one isotope.
Stable oxygen atoms that have the same atomic number as the element oxygen, but differ in atomic weight. O-17 and 18 are stable oxygen isotopes.
Communication between animals involving the giving off by one individual of some chemical or physical signal, that, on being received by another, influences its behavior.
An enzyme that catalyzes the reaction between a purine nucleoside and orthophosphate to form a free purine plus ribose-5-phosphate. EC 2.4.2.1.
Pheromones that elicit sexual attraction or mating behavior usually in members of the opposite sex in the same species.
Nucleosides in which the base moiety is substituted with one or more sulfur atoms.
Chemical substances, excreted by an organism into the environment, that elicit behavioral or physiological responses from other organisms of the same species. Perception of these chemical signals may be olfactory or by contact.
The repeating structural units of chromatin, each consisting of approximately 200 base pairs of DNA wound around a protein core. This core is composed of the histones H2A, H2B, H3, and H4.
Small chromosomal proteins (approx 12-20 kD) possessing an open, unfolded structure and attached to the DNA in cell nuclei by ionic linkages. Classification into the various types (designated histone I, histone II, etc.) is based on the relative amounts of arginine and lysine in each.
A mass spectrometry technique using two (MS/MS) or more mass analyzers. With two in tandem, the precursor ions are mass-selected by a first mass analyzer, and focused into a collision region where they are then fragmented into product ions which are then characterized by a second mass analyzer. A variety of techniques are used to separate the compounds, ionize them, and introduce them to the first mass analyzer. For example, for in GC-MS/MS, GAS CHROMATOGRAPHY-MASS SPECTROMETRY is involved in separating relatively small compounds by GAS CHROMATOGRAPHY prior to injecting them into an ionization chamber for the mass selection.
The material of CHROMOSOMES. It is a complex of DNA; HISTONES; and nonhistone proteins (CHROMOSOMAL PROTEINS, NON-HISTONE) found within the nucleus of a cell.
An analytical method used in determining the identity of a chemical based on its mass using mass analyzers/mass spectrometers.
A widely used local anesthetic agent.
A local anesthetic and cardiac depressant used as an antiarrhythmia agent. Its actions are more intense and its effects more prolonged than those of PROCAINE but its duration of action is shorter than that of BUPIVACAINE or PRILOCAINE.
Drugs that block nerve conduction when applied locally to nerve tissue in appropriate concentrations. They act on any part of the nervous system and on every type of nerve fiber. In contact with a nerve trunk, these anesthetics can cause both sensory and motor paralysis in the innervated area. Their action is completely reversible. (From Gilman AG, et. al., Goodman and Gilman's The Pharmacological Basis of Therapeutics, 8th ed) Nearly all local anesthetics act by reducing the tendency of voltage-dependent sodium channels to activate.
Deuterium. The stable isotope of hydrogen. It has one neutron and one proton in the nucleus.
Procedure in which an anesthetic is injected directly into the spinal cord.

Impact of 9-(2-phosphonylmethoxyethyl)adenine on (deoxy)ribonucleotide metabolism and nucleic acid synthesis in tumor cells. (1/3036)

Following exposure to 9-(2-phosphonylmethoxyethyl)adenine (an inhibitor of the cellular DNA polymerases alpha, delta and epsilon), human erythroleukemia K562, human T-lymphoid CEM and murine leukemia L1210 cells markedly accumulated in the S phase of the cell cycle. In contrast to DNA replication, RNA synthesis (transcription) and protein synthesis (mRNA translation) were not affected by 9-(2-phosphonylmethoxyethyl)-adenine. The ribonucleoside triphosphate pools were slightly elevated, while the intracellular levels of all four deoxyribonucleoside triphosphates were 1.5-4-fold increased in 9-(2-phosphonylmethoxyethyl)adenine-treated K562, CEM and L1210 cells. The effect of 9-(2-phosphonylmethoxyethyl)adenine on de novo (thymidylate synthase-mediated) and salvage (thymidine kinase-mediated) dTTP synthesis was investigated using radio-labelled nucleoside precursors. The amount of thymidylate synthase-derived dTTP in the acid soluble pool was 2-4-fold higher in PMEA-treated than in untreated K562 cells, which is in accord with the 3-4-fold expansion of the global dTTP level in the presence of 9-(2-phosphonylmethoxyethyl)adenine. Strikingly, 2-derived dTTP accumulated to a much higher extent (i.e. 16-40-fold) in the soluble dTTP pool upon 9-(2-phosphonylmethoxyethyl)adenine treatment. In keeping with this finding, a markedly increased thymidine kinase activity could be demonstrated in extracts of 9-(2-phosphonylmethoxyethyl)adenine-treated K562 cell cultures. Also, in the presence of 200 microM 9-(2-phosphonylmethoxyethyl)adenine, 14-fold less thymidylate synthase-derived but only 3-fold less thymidine kinase-derived dTTP was incorporated into the DNA of the K562 cells. These data show that thymidine incorporation may be inappropriate as a cell proliferation marker in the presence of DNA synthesis inhibitors such as 9-(2-phosphonylmethoxyethyl)adenine. Our findings indicate that 9-(2-phosphonylmethoxyethyl)adenine causes a peculiar pattern of (deoxy)ribonucleotide metabolism deregulation in drug-treated tumor cells, as a result of the metabolic block imposed by the drug on the S phase of the cell cycle.  (+info)

Transfer of the 1-pro-R and the 1-pro-S hydrogen atoms of ethanol in metabolic reductions in vivo. (2/3036)

The transfer of deuterium from [1 R-2H]ethanol and [1 S-2H]-ethanol to reduced metabolites of administered compounds was measured in female rats provided with bile fistulas. Administered cyclohexanone was reduced to cyclohexanol, and in this reduction hydrogen was transferred only from the 1-pro-R position of the ethanol. The deuterium content in the cyclohexanol was about 67% of that in the ethanol. In the reduction of the 17-oxo group in 3beta-hydroxy-5alpha-androstan-17-one, hydrogen was transferred both from the 1-pro-R position and the 1-pro-S position, resulting in degrees of labelling that were about 25% and 2%, respectively, of those in the specific positions of the ethanols. The 1-pro-R and 1-pro-S positions of ethanol contributed about 9% and 5%, respectively, of the 3beta hydrogen in lithocholic acid formed from 3-oxo-5beta-cholanoic acid. The results indicate that alcohol dehydrogenase and aldehyde dehydrogenase do not share a common pool of NAD, and that NADH formed during acetaldehyde oxidation is utilized for reductions in the cytosol to a smaller extent than the NADH formed in the alcohol dehydrogenase reaction. This result supports the concept that aldehyde oxidation is mainly an intramitochondrial process. The relatively extensive utilization of the 1-pro-S hydrogen of ethanol in the reduction of 3-oxo-5beta-cholanoic acid, that is probably NADPH-dependent, indicates that cytosolic NADPH may be produced from malate or isocitrate formed intramitochondrially.  (+info)

Kinetics of thyroglobulin iodination and of hormone synthesis catalysed by thyroid peroxidase. Role of iodide in the coupling reaction. (3/3036)

The kinetics of tyrosine iodination and of thyroxine synthesis in thyroglobulin, different reactions catalyzed by the same enzyme (thyroid peroxidase), have been compared. Thyroxine synthesis always began after a lag period of 3-5 min. This lag was constant whatever the rate of iodination; this rate of iodination was increased either by increasing the concentration of iodide or enzyme or by decreasing the concentration of thyroglobulin. Increasing the rate of iodination resulted in increasing the number of iodine atoms incorporated during the lag period. Thus the lag observed for thyroxine synthesis was constant and did not depend on the fact that free iodide or non-iodinated tyrosine residues of thyroglobulin were exhausted before thyroxine synthesis occurred. Finally, it appeared that, whatever the explanation of the lag, the enzyme catlyzes thyroid hormone synthesis at a slower rate than iodination. The existence of a lag also allowed us to prepare thyroglobulin samples with different iodine contents but without thyroid hormones. Thus iodination and thyroxine synthesis could be studied independently and the following results were obtained. 1. Iodotyrosine residues which can couple to form thytoxine are made considerably before coupling occurs. 2. H2O2 is required for coupling of these hormonogenic residues; thus the coupling reaction requires enzymic oxidation of the iodotyrosine residues. 3. In addition a strict requirement for iodide was needed for coupling; the requirement was dependent on the concentration of iodide. Thus iodide, a substrate of the iodination reaction, may also have other effects on the activity of thyroid peroxidase.  (+info)

Quantitative determination of N-acetylglucosamine residues at the non-reducing ends of peptidoglycan chains by enzymic attachment of [14C]-D-galactose. (4/3036)

The ability of human milk galactosyltransferase to attach D-galactose residues quantitatively to the C-4 of N-acetylglucosamine moieties at the ends of oligosaccharides has been utilized for the specific labeling and quantitative determination of the chain length of the glycan moiety of the bacterial cell wall. The average polysaccharide chain length of the soluble, uncrosslinked peptidoglycan secreted by Micrococcus luteus cells on incubation with penicillin G was studied with this technique and found to be approximately 70 hexosamines long. Furthermore, the peptidoglycan chain length of Escherichia coli sacculi of different cell shapes and dimensions was determined both in rod-shaped cells and in filaments induced by temperature shift of a division mutant or by addition of cephalexin or nalidixic acid. The average chain length found in most of these sacculi was between 70 and 100 hexosamines long. Small spherical 'mini' cells had chain lengths similar to those of the isogenic rod-like cells.  (+info)

Radioactive labelling of ribosomal proteins with reductive alkylation and its use in studying ribosome-cytosol interactions. (5/3036)

Mouse brain ribosomes were radioactively labelled by a cell-free reductive alkylation reaction with NaBH4 and [14C]formaldehyde. The radioactivity was largely associated with ribosomal proteins, but little, if any, of the rRNA was radioactive after the alkylation procedure. Both ribosomal structural proteins and loosely associated components were successfully labelled by this procedure. The sedimentation properties of the ribosomes were unaltered and their ability to carry out poly(U)-directed protein synthesis, although decreased, was largely retained. Incubation of 14C-labelled ribosomes with brain cytosol resulted in a 17% loss of radioactivity, although treatment of the ribosomes with 1.0 m-KCl to remove the loosely associated factors rendered the ribonucleoprotein particles resistant to cytosol effects. The ribosome-cytosol interactions did not appear to be related to an exchange process, since the released radioactivity was largely degraded to acid-soluble material. In addition, the incubation of native ribosomes with brain cytosol resulted in an almost complete loss in the ability of the ribosomes to participate in cell-free protein synthesis.  (+info)

Intratumoral distribution of two consecutive injections of chimeric antibody G250 in primary renal cell carcinoma: implications for fractionated dose radioimmunotherapy. (6/3036)

Tumor uptake of the chimeric G250 (cG250) monoclonal antibody (mAb) in patients with primary renal cell carcinoma (RCC) is among the highest reported in solid tumors. However, as observed in other tumor types, the intratumoral distribution of the antibody is highly heterogeneous, which may limit the efficacy of radioimmunotherapy. A number of highly dynamic physiological factors have been postulated that may contribute to heterogeneous tumor uptake of antibodies. Their impact on tumor uptake of antibodies may vary from one tumor region to another as well as from one day to the next. Here, we report on a clinical study that was designed to investigate whether the pattern of mAb cG250 uptake within RCC tumors is altered with subsequent injections. Ten patients with a clinical diagnosis of primary RCC were studied. Nine days before surgery, patients received 125I-cG250 (5 mg of cG250, 50 microCi of 125I), followed by a second injection of 131I-cG250 (5 mg of cG250, 3.5 mCi of 131I) 4 days later. Postsurgery, the tumor was cut into (1-cm) thick slices. Slices were imaged on a gamma camera, and the slice with the most pronounced heterogeneity in 131I-cG250 distribution was selected and cut into 1-cm3 cubes. Each cube was analyzed for 121I-cG250 and 131I-cG250 uptake, and the 131I/125I ratio was determined. For each tumor slice, the distribution patterns of both isotopes were reconstructed and compared with each other. All tumors analyzed showed a heterogeneous distribution of both isotopes throughout the tumor slice; focal uptake in some areas of a tumor reached very high levels (up to 0.19% injected dose/g), whereas other tumorous areas of the same slice showed much lower uptake (as low as 0.0047% injected dose/g). Remarkably, in all tumors, the distribution pattern of both injections was identical: without exception, in all samples analyzed (n = 692), the uptake of 125I-cG250 was similar to 131I-cG250 uptake. Overall, the 131I/125I ratio was 1.64+/-0.31 (mean+/-SD). The constant 131I/125I ratios, observed in all tumor samples investigated, indicate that the tumor parameters governing cG250 mAb uptake were not altered significantly within the time period studied. In addition, the results of this study suggest that multiple radiolabeled antibody injections, administered within short time periods, will target the same areas within a tumor and, thus, will not solve the problem of heterogeneous tumor uptake of antibody.  (+info)

Structures of the M2 channel-lining segments from nicotinic acetylcholine and NMDA receptors by NMR spectroscopy. (7/3036)

The structures of functional peptides corresponding to the predicted channel-lining M2 segments of the nicotinic acetylcholine receptor (AChR) and of a glutamate receptor of the NMDA subtype (NMDAR) were determined using solution NMR experiments on micelle samples, and solid-state NMR experiments on bilayer samples. Both M2 segments form straight transmembrane alpha-helices with no kinks. The AChR M2 peptide inserts in the lipid bilayer at an angle of 12 degrees relative to the bilayer normal, with a rotation about the helix long axis such that the polar residues face the N-terminal side of the membrane, which is assigned to be intracellular. A model built from these solid-state NMR data, and assuming a symmetric pentameric arrangement of M2 helices, results in a funnel-like architecture for the channel, with the wide opening on the N-terminal intracellular side.  (+info)

15N-labelling and preliminary heteronuclear NMR study of Desulfovibrio vulgaris Hildenborough cytochrome c553. (8/3036)

When using heteronuclear NMR, 15N-labelling is necessary for structural analysis, dynamic studies and determination of complex formation. The problems that arise with isotopic labelling of metalloproteins are due to their complex maturation process, which involves a large number of factors. Cytochromes c are poorly expressed in Escherichia coli and the overexpression that is necessary for 15N-labelling, requires an investigation of the expression host and special attention to growth conditions. We have succeeded in the heterologous expression and the complete and uniform isotopic 15N-labelling of the cytochrome c553 from Desulfovibrio vulgaris Hildenborough, in a sulphate-reducing bacterium, D. desulfuricans G200, by using a growth medium combining 15N-ammonium chloride and 15N-Celtone. These conditions allowed us to obtain approximately 0.8 mg x L-1 of pure labelled cytochrome c553. 1H and 15N-assignments for both the oxidized and the reduced states of cytochrome c553 were obtained from two-dimensional heteronuclear experiments. Pseudocontact effects due to the haem Fe3+ have been analysed for the first time through 15N and 1H chemical shifts in a c-type cytochrome.  (+info)

An initial step in the systematic investigation of cellular processes is the identification and measurement of expression levels of relevant sets of proteins. Recently, quantitative approaches utilizing MS and a host of stable isotope-labeling chemistries have emerged (reviewed in Refs. 1 and 2), offering a departure from traditional techniques employing comparative two-dimensional gel electrophoresis. The ICAT quantitative labeling strategy (3, 4) is perhaps the best-characterized method for relative protein quantitation using MS. Other elegant approaches use cell-culture enrichment with a stable isotope-labeled amino acid, including arginine (5), lysine (6), tyrosine (7), and leucine (8), for in vivo incorporation of a mass difference to support relative quantitation. This circumvents potential difficulties surrounding chemical labeling downstream in a comparative experiment. All of these methods impart a mass difference as the basis for quantitation by measurement of relative peak areas of MS ...
The first ice core samples date from the 1960s, when more became known in the world of physics about how water molecules with heavy isotopes transform from water into water vapour in the air. This occurs during the process where water evaporates in the tropics to eventually fall as rain or snow at higher latitudes.. The Groningen physicist Prof. Harro Meijer has been involved in climate research for a long time now, as head of the Universitys Centre for Isotope Research (CIO). The water vapour slowly loses heavy isotopes during that journey because precipitation tends to include that type of isotope, he explains when talking about the principle behind the research. When it arrives at the North Pole, the remaining water vapour contains fewer heavy isotopes. How much has been lost exactly depends on the temperature on the way. So once you know how much has been lost, you can in principle calculate the temperature.. ...
Where R is the ratio of the heavy to light isotope, u is the unknown or sample, and std is the international standard. The commonly used standard for 2H and 18O in water is Standard Mean Ocean Water (SMOW). A relative abundance of 0 is identical with SMOW, a negative relative abundance indicates less heavy isotope than SMOW, and a positive relative abundance indicates more heavy isotope than SMOW. Recast in per mil units, the natural range of deuterium abundances is 450 to +50 ; and that of 18O is -60 to +50 ; (Figure 8.1).. These natural variations result from the accumulation of isotope effect as these elements are cycled through the hydro and biospheres. Of these natural variations, those of water have the greatest influence on the doubly-labelled water method. This is because water is the major source of hydrogen and oxygen that flows into body water and it has the major influence on the isotopic abundances of 2H and 18O in body water. Water is ingested either as a beverage or as moisture in ...
A free platform for explaining your research in plain language, and managing how you communicate around it - so you can understand how best to increase its impact.
Metabolic labeling is routinely performed with cultured cells (SILAC), ranging from bacteria and yeast to mammalian cells. However, analysis of mammalian tissue allows greater insight into physiology compared to cultured cells. Stable Isotope Labeling of Mammals (SILAM) refers to the in-vivo incorporation of heavy isotopes in living organisms such as rodents. SILAM enables global, relative quantitative analysis of mammalian disease models and provides insights on post-translational modifications (PTMs). In addition, the labeling of an entire proteome generates ideal standards for quantitative proteomics.
The current focus of many studies in proteomics is to determine the changes in levels of individual proteins; in this respect stable isotope labels can be of considerable value in comparative studies. However, a very different outcome of stable isotope labeling is obtained if the exposure to precursor is of such a short time that labeling is incomplete. Under these circumstances, the extent of labeling of peptides in single proteins is dictated by the growth rate of the system, and the rate of intracellular turnover of the protein. Short labeling windows can therefore access the turnover rates of individual proteins, and because these proteins can be subsequently isolated (whether by gel-based methods, by LC separation of peptides, or by alternative approaches to proteome simplification), it is now feasible to measure the rate of turnover of individual proteins in the proteome. This information is going to become increasingly important as systems biology thinking starts to integrate ...
The experiments involved in the PHENIX french nuclear reactor to obtain precise and accurate data on the total capture cross sections of the heavy isotopes
You could grow some bacteria in a food source that contains only heavy isotope of nitrogen - N-15. After many generations, the DNA should only contain the heavy isotope of nitrogen. Some bacteria can be transferred to another food source containing the normal lighter isotope of nitrogen - N-14. The DNA can be extracted from the bacteria and separated e.g. centrifuged. The more dense, heavier molecules (N-15) will stay at the bottom. it could be centrifuged bu mixing the DNA sample with cocentrated sugar solution then placing the mixture of DNA and sugar solution into test tubes and spinning them at high ...
Peptides , ClearPoint -Heavy Isotope Labeled and Related Peptides , ClearPoint beta-Amyloid (1-38), 13C-Phe & Ile, Human; This peptide is beta-amyloid (1-38) with phenylalanine and isoleucine universally labeled with 13C. Ab is found in amyloid deposits of Alzheimer s patients and is implicated in the pathogenesis of this disease.; DAEFRHDSGYEVHHQKLV-*F-*F-AEDVGSNKGA-*I-*I-GLMVGG *F=Phe(U-13C9), *I=Ile(U-13C6); H-Asp-Ala-Glu-Phe-Arg-His-Asp-Ser-Gly-Tyr-Glu-Val-His-His-Gln-Lys-Leu-Val-*Phe-*Phe-Ala-Glu-Asp-Val-Gly-Ser-Asn-Lys-Gly-Ala-**Ile-**Ile-Gly-Leu-Met-Val-Gly-Gly-OH *Phe(U-13C9); **Ile(U-13C6)
Logarithmized SILAC proteins of been feet from compositional and different conformations knew limited. The meaning is the also opposed publishing of SILAC papers of conversational life foundations in Sirt3 new interactions. like download the complete mrcpsych was deferred rising Wilcoxon audio analysis incorporation.
Zhou J (2008) CST Curation Set: 3811; Year: 2008; Biosample/Treatment: tissue, brain/untreated; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: m1R ...
Mulhern D (2012) CST Curation Set: 15846; Year: 2012; Biosample/Treatment: tissue, embryo/untreated; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: mR ...
Patient information for STANNOUS AGENT 4 MILLIGRAMS/6.8 MILLIGRAMS KIT FOR RADIOPHARMACEUTICAL PREPARATION Including dosage instructions and possible side effects.
A Photographic Investigation of the Transmutation of Lithium and Boron by Protons and of Lithium by Ions of the Heavy Isotope of Hydrogen ...
Semi-conservative replication of DNA was proved by the work of Mathew Meselson and Franklin Stahl (1958). They grew Escherichia coli for many generations in a medium having heavy isotope of nitrogen, in the form of 15NH4Cl, till the bacterial DNA became com-pletely labelled with heavy isotope.. The labelled bacteria were then shifted to fresh medium hav-ing normal or 14N nitrogen. Samples were taken for each generation (one generation takes 20 minutes as E. coli divides in 20 minutes) and the DNA was tested for the heavy isotope of nitrogen through density gradient centrifugation using caesium chloride. Caesium chloride is highly water soluble heavy salt.. When spun in centrifuge at high speed (say 50,000 revolutions per minute) the salt forms a density gradient with heaviest most concentrated region at the bottom and successively less concentrated lighter one towards the surface. When DNA is mixed with caesium chloride it will settle down at a particular height in centrifugation, heavier ...
The abundance of different isotopes in a system are usually reported as isotope ratio R or more commonly in the delta notation relative to the isotope ratio of reference material (see Chapter 2) where HA is the amount of heavy isotope in the system, LA the amount of the light isotopes, and Rstd the isotope ratio of the reference material. Since variations of isotope ratios are often small, the 5-values are usually expressed on a per mill scale by multiplication with 1000. For example a value of.... ...
TY - JOUR. T1 - Site-specific radioiodination of HER2-targeting affibody molecules using 4-iodophenethylmaleimide decreases renal uptake of radioactivity. AU - Strand, Joanna. AU - Nordeman, Patrik. AU - Honarvar, Hadis. AU - Altai, Mohamed. AU - Orlova, Anna. AU - Larhed, Mats. AU - Tolmachev, Vladimir. N1 - Publisher Copyright: © 2014 The Authors. Published by Wiley-VCH Verlag GmbH & Co. KGaA. Copyright: Copyright 2017 Elsevier B.V., All rights reserved.. PY - 2015/4/1. Y1 - 2015/4/1. N2 - Affibody molecules are small scaffold-based affinity proteins with promising properties as probes for radionuclide-based molecular imaging. However, a high reabsorption of radiolabeled Affibody molecules in kidneys is an issue. We have shown that the use of 125I-3-iodo-((4-hydroxyphenyl)ethyl)maleimide (IHPEM) for site-specific labeling of cysteine-containing Affibody molecules provides high tumor uptake but low radioactivity retention in kidneys. We hypothesized that the use of 4-iodophenethylmaleimide ...
But lets be clear, thats all hes doing. Hes proposing an hypothesis. His big idea is to feed livestock with deuterium-enriched feed, eat a load of meat produced in this way, and end up living longer. But all this is just an idea - he hasnt actually fed pigs deuterium, and theres no data available to back up the theory that eating deuterium-laden pork will enhance lifespan - indeed, I cant find the data the Mail article refers to involving worms and fruitflies (Im happy to be corrected though...). Just because heavy isotopes appear to be resilient to oxidative damage in a laboratory setting, does that mean well live longer if we drink heavy water? The point is we dont know, and Shchepinov doesnt either - he just had an idea, but the Mail reported it as though havey water really could be the best way to prevent ageing. Again, just to emphasise, this is not a story based on research into how heavy isotopes extend life - its based on thinking that heavy isotopes could extend life ...
Thermo Scientific™ Pierce™ SILAC Media and Sera RPMI, powdered; 104g Thermo Scientific™ Pierce™ SILAC Media and Sera SILAC Isotope...
Affibody molecules have lately shown great potential as tools for in vivo molecular imaging. These small, 3-helical bundles, with their highly stable protein scaffold, are well suited for the often harsh conditions of radiolabeling. Their small size allows for rapid clearance from the blood circulation which permits the collection of images already within hours after injection. This thesis includes four papers aimed at engineering different variants of a HER2-binding Affibody molecule to enable effective and flexible radiolabeling and enhancing the molecular imaging in terms of imaging contrast and resolution.. In paper I an Affibody molecule was engineered to function as a multifunctional platform for site-specific labeling with different nuclides for radionuclide imaging. This was done using only natural amino acids, thereby allowing for both synthetic and recombinant production. By grafting the amino acid sequence -GSECG to the C-terminal of our model-protein, a HER2-binding Affibody ...
[116 Pages Report] Check for Discount on Europe Stable Isotopes Market by Manufacturers, Countries, Type and Application, Forecast to 2022 report by Global Info Research. This report studies the Stable Isotopes market, stable isotopes included...
Isotopes of an element have the same atomic number, but different atomic mass. Stable isotopes do not undergo radioactive decay as radio-isotopes do. Elements can exist in both stable and unstable (radioactive) forms. Most elements of biological interest (including C, H, O, N, and S) have two or more stable isotopes, with the lightest of these present in much greater abundance than the others. Among stable isotopes the most useful as biological tracers are the heavy isotopes of hydrogen, carbon and nitrogen. These elements are found in the earth, the atmosphere, and all organisms ...
Stadlmeier, M. ; Bogena, J. ; Wallner, M. ; Wühr, M. ; Carell, T. A sulfoxide-based isobaric labelling reagent for accurate quantitative mass spectrometry. Angewandte Chemie 2018.
Protein quantitation using Ru-NHS ester tagging and isotope dilution high-pressure liquid chromatography-inductively coupled plasma mass spectrometry determination
Protein stable isotope probing (protein-SIP) has strong potential for revealing key metabolizing taxa in complex microbial communities. While most protein-SIP work to date has been performed under controlled laboratory conditions to allow extensive isotope labeling of the target organism, a key application will be in situ studies of microbial communities under conditions that result in small degrees of partial labeling. One hurdle restricting large scale in situ protein-SIP studies is the lack of algorithms and software for automated data processing of the massive data sets resulting from such studies. In response, we developed Stable Isotope Probing Protein Extraction Resources software (SIPPER) and applied it for large scale extraction and visualization of data from short term (3 h) protein-SIP experiments performed in situ on Yellowstone phototrophic bacterial mats. Several metrics incorporated into the software allow it to support exhaustive analysis of the complex composite isotopic ...
The stellar reaction rates of radiative alpha-capture reactions on heavy isotopes are of crucial importance for the gamma process network calculations. These rates are usually derived from statistical model calculations, which need to be validated, but the experimental database is very scarce. This paper presents the results of alpha-induced reaction cross section measurements on iridium isotopes carried out at first close to the astrophysically relevant energy region. Thick target yields of Ir-191(alpha,gamma)Au-195, Ir-191(alpha,n)Au-194, Ir-193(alpha,n)Au-196m, Ir-193(alpha,n)Au-196 reactions have been measured with the activation technique between E-alpha = 13.4 MeV and 17 MeV. For the first time the thick target yield was determined with X-ray counting. This led to a previously unprecedented sensitivity. From the measured thick target yields, reaction cross sections are derived and compared with statistical model calculations. The recently suggested energy-dependent modification of the ...
Free amino acids. Stable isotopes for metabolic studies. CortecNet is a Stable Isotopes-driven company. We produce over than 2000 innovative labeled compounds with stable isotopes (13C, 15N, D).
Isotopes may be referred to in the medical literature alone or as a component of a radiopharmaceutical administered for therapeutic or diagnostic purposes. The nomenclature for the isotopes incorporated in radiopharmaceuticals follows the international nonproprietary name (INN) drug nomenclature and therefore differs from that of isotopes that occur as elements alone. | An isotope referred to as an element rather than as part of the name of a chemical compound may be described at first mention by providing the name of the element spelled out followed by the isotope number in the same typeface and type size (no hyphen, subscript,
Isotopes may be referred to in the medical literature alone or as a component of a radiopharmaceutical administered for therapeutic or diagnostic purposes. The nomenclature for the isotopes incorporated in radiopharmaceuticals follows the international nonproprietary name (INN) drug nomenclature and therefore differs from that of isotopes that occur as elements alone. | An isotope referred to as an element rather than as part of the name of a chemical compound may be described at first mention by providing the name of the element spelled out followed by the isotope number in the same typeface and type size (no hyphen, subscript,
Molecular imaging is an emerging multidisciplinary field that addresses the visualisation of diseases at the cellular and molecular levels. This thesis focuses on the development of a synthetic Affibody molecule-based imaging tracer for the detection of HER2 expression in malignant tumours.. Papers I-IV report the development of the HER2-specific Affibody molecule, ZHER2:342 by peptide synthesis and the use of different chelators attached to the N-terminus to allow 99mTc-labelling. Paper I described the optimisation of labelling of Affibody molecules using cysteine-based chelator sequences, in which the direct labelling method under alkaline conditions was the most suitable one. Papers II-IV report the development and optimisation of the in vivo properties of the HER2-specific Affibody molecule for high-contrast imaging. By using an array of mercaptoacetyl-based chelators, it was found that the substitution of a single amino acid in a 60 amino acid-long Affibody molecule can dramatically change ...
Isotopes can be thought of as different flavours of a particular element (such as oxygen or carbon), that are distinguished by the number of neutrons in their nucleus (and hence their atomic mass). Carbon for instance most commonly has a mass of 12 (written as 12C), but there are also a small fraction of carbon atoms with mass 13 and 14 (13C and 14C), similarly oxygen is normally 16O, but with small amounts of 17O and 18O. All of the isotopes of an element behave in similar way chemically. However, because the mass of each isotope is slightly different there are certain physical processes that will discriminate (or fractionate) between them. For instance, during evaporation of water, it is slightly easier for the lighter isotopes to escape from the liquid, and so water vapour generally has less 18O than the liquid water from which it came. Because of these physical effects, looking at the ratio of one isotope to another can often be very useful in tracing where these atoms came from.. ...
Cambridge Isotope Laboratories offers a complete listing of Stable Isotope Labeled Deuterated Solvents for Dissolution DNP for all your research needs. View pricing, availability and product specifications.
Our isotope-labeling experiments revealed that respiration through the TCA cycle is enhanced in the srk2d srk2e srk2i mutant (Figure 6). Stable-isotope labeling with [U-13C]-Glc provided greater resolution at the individual metabolite level, revealing greater incorporation of label into citrate in srk2d srk2e srk2i compared with wild type, but no significant differences between genotypes in the labeling of succinate and malate (Figure 7). In addition, citrate, aconitate, and isocitrate were highly accumulated in srk2d srk2e srk2i, whereas the amounts of other intermediates were unchanged (Figures 3 and 8). These results are consistent with previous reports that the TCA cycle can operate in noncyclic flux modes in illuminated leaves (Tcherkez et al., 2009; Sweetlove et al., 2010). Given that pyruvate dehydrogenase is inhibited in the light (Tcherkez et al., 2005), a noncyclic flux runs from oxaloacetate via malate, pyruvate, and acetyl CoA to citrate. Following this proposed model, our results ...
Medical Isotopes, Inc. a premier manufacturer of stable isotope chemicals labeled with: Deuterium, C13, N15, O18 and metal isotopes. We specialize in custom synthesis with isotopes and non-labeled compounds.
Medical Isotopes, Inc. a premier manufacturer of stable isotope chemicals labeled with: Deuterium, C13, N15, O18 and metal isotopes. We specialize in custom synthesis with isotopes and non-labeled compounds.
Chris Knowles of Oregon State University and I got into some SERIOUS chemistry last week. Lets see how it applies to the wood industry now.. Chris, you gave us a chemistry lesson last week. How do we USE this stable isotope analysis in the real world?. Among other uses, stable isotope analysis has a relatively long history of use for dating materials. Carbon dating provides an estimation of generally ±50 years for samples less than about 10,000 years old. However, the older the material, the less accurate the estimation will be. This estimate is based on a number of assumptions that may or may not be accurate. The bottom line is that this type of analysis will not identify the exact age of any material. It will only provide an estimate.. More recently, scientists have been exploring the potential to use the natural variability in stable isotopes by region to determine geographic origin of natural materials, including wood products. While the chemical analysis has a long history of use in other ...
Drosophila melanogaster is a common animal model for genetics studies, and quantitative proteomics studies of the fly are emerging. Here, we present in detail the development of a procedure to incorporate stable isotope-labeled amino acids into the fly proteome. In the method of stable isotope labeling with amino acids in Drosophila melanogaster (SILAC fly), flies were fed with SILAC-labeled yeast grown with modified media, enabling near complete labeling in a single generation. Biological variation in the proteome among individual flies was evaluated in a series of null experiments. We further applied the SILAC fly method to profile proteins from a model of fragile X syndrome, the most common cause of inherited mental retardation in human. The analysis identified a number of altered proteins in the disease model, including actin-binding protein profilin and microtubulin-associated protein futsch. The change of both proteins was validated by immunoblotting analysis. Moreover, we extended the ...
Drosophila melanogaster is a common animal model for genetics studies, and quantitative proteomics studies of the fly are emerging. Here, we present in detail the development of a procedure to incorporate stable isotope-labeled amino acids into the fly proteome. In the method of stable isotope labeling with amino acids in Drosophila melanogaster (SILAC fly), flies were fed with SILAC-labeled yeast grown with modified media, enabling near complete labeling in a single generation. Biological variation in the proteome among individual flies was evaluated in a series of null experiments. We further applied the SILAC fly method to profile proteins from a model of fragile X syndrome, the most common cause of inherited mental retardation in human. The analysis identified a number of altered proteins in the disease model, including actin-binding protein profilin and microtubulin-associated protein futsch. The change of both proteins was validated by immunoblotting analysis. Moreover, we extended the ...
Enhanced qualitative and quantitative proteomic analysis using pSMART combines data dependent and data independent acquisition for improved results.
name=full demo , conventional atomic weight= , standard atomic weight=238.02891(3) , standard atomic weight ref=,ref,Reference for A,sub,r,/sub,,/ref, , isotopes table footnote=* = [[excited state]]{{sfn,Chisté,2006}} , isotopes = {{Infobox element isotopes/isotopes stable , link=Fluorine-19 , mn=19 , sym=F , na=100% , n=10 }} {{Infobox element isotopes/isotopes decay , mn=251 , sym=Cf , na=trace , hl=898 y , dm=α , de=6.172 , link1=curium-247 , pn=247 , ps=Cm }} {{Infobox element isotopes/isotopes decay2 , mn=252 , sym=Cf , na=trace , hl=2.645 y , dm1=α (96.91%) , de1=6.217 , link1=curium-248 , pn1=248 , ps1=Cm , dm2=SF (3.09%) , de2=- , pn2= , ps2=- }} {{Infobox element isotopes/isotopes decay3 , mn=26 , sym=Al , na=[[trace radioisotope,trace]] , hl=7.17×105 y , dm1=[[Positron emission,β,sup,+,/sup,]] , de1=1.17 , link1=magnesium-26 , pn1=26 , ps1=Mg , dm2=[[electron capture,ε]] , de2=- , link2=magnesium-26 , pn2=26 , ps2=Mg , dm3=[[Gamma radiation,γ]] , de3=1.8086 , pn3= , ps3=- }} ...
name=full demo , conventional atomic weight= , standard atomic weight=238.02891(3) , standard atomic weight ref=,ref,Reference for A,sub,r,/sub,,/ref, , isotopes table footnote=* = [[excited state]]{{sfn,Chisté,2006}} , isotopes = {{Infobox element isotopes/isotopes stable , link=Fluorine-19 , mn=19 , sym=F , na=100% , n=10 }} {{Infobox element isotopes/isotopes decay , mn=251 , sym=Cf , na=trace , hl=898 y , dm=α , de=6.172 , link1=curium-247 , pn=247 , ps=Cm }} {{Infobox element isotopes/isotopes decay2 , mn=252 , sym=Cf , na=trace , hl=2.645 y , dm1=α (96.91%) , de1=6.217 , link1=curium-248 , pn1=248 , ps1=Cm , dm2=SF (3.09%) , de2=- , pn2= , ps2=- }} {{Infobox element isotopes/isotopes decay3 , mn=26 , sym=Al , na=[[trace radioisotope,trace]] , hl=7.17×105 y , dm1=[[Positron emission,β,sup,+,/sup,]] , de1=1.17 , link1=magnesium-26 , pn1=26 , ps1=Mg , dm2=[[electron capture,ε]] , de2=- , link2=magnesium-26 , pn2=26 , ps2=Mg , dm3=[[Gamma radiation,γ]] , de3=1.8086 , pn3= , ps3=- }} ...
Lung cancer is the most common cause of cancer-related death worldwide, less than 7% of patients survive 10 years following diagnosis across all stages of lung cancer. Late stage of diagnosis and lack of effective and personalized medicine reflect the need for a better understanding of the mechanisms that underlie lung cancer progression. Quantitative proteomics provides the relative different protein abundance in normal and cancer patients which offers the information for molecular interactions, signaling pathways, and biomarker identification. Here we introduce both theoretical and practical applications in the use of quantitative proteomics approaches, with principles of current technologies and methodologies including gel-based, label free, stable isotope labeling as well as targeted proteomics. Predictive markers of drug resistance, candidate biomarkers for diagnosis, and prognostic markers in lung cancer have also been discovered and analyzed by quantitative proteomic analysis. Moreover,
The site-specific quantitation of N- and O-glycosylation is vital to understanding the function(s) of different glycans expressed at a given site of a protein under physiological and disease conditions. Most commonly used precursor ion intensity based quantification method is less accurate and other labeled methods are expensive and require enrichment of glycopeptides. Here, we used glycopeptide product (y and Y0) ions and (18)O-labeling of C-terminal carboxyl group as a strategy to obtain quantitative information about fold-change and relative abundance of most of the glycoforms attached to the glycopeptides. As a proof of concept, the accuracy and robustness of this targeted, relative quantification LC-MS method was demonstrated using Rituximab. Furthermore, the N-glycopeptide quantification results were compared with a biosimilar of Rituximab and validated with quantitative data obtained from 2-AB-UHPLC-FL method. We further demonstrated the intensity fold-change and relative abundance of 46 ...
ISOFLEX supplies stable and radioactive isotopes. We lead in pricing, enrichment, processing, and customer service. ISOFLEX is your premier isotope supplier.
ISOFLEX supplies stable and radioactive isotopes. We lead in pricing, enrichment, processing, and customer service. ISOFLEX is your premier isotope supplier.
TY - JOUR. T1 - Measurement of de novo hepatic lipogenesis in humans using stable isotopes. AU - Hellerstein, M. K.. AU - Christiansen, M.. AU - Kaempfer, S.. AU - Kletke, C.. AU - Wu, K.. AU - Reid, J. S.. AU - Mulligan, K.. AU - Hellerstein, N. S.. AU - Shackleton, C. H L. PY - 1991/5. Y1 - 1991/5. N2 - Direct measurement of de novo lipogenesis has not previously been possible in humans. We measured de novo hepatic lipogenesis in normal men by means of stable isotopes and by combining the acetylated-xenobiotic probe technique with mass isotopomer analysis of secreted very low density lipoprotein-fatty acids (VLDL-FA). Sulfamethoxazole (SMX) was administered with [13C]acetate during an overnight fast followed by refeeding with intravenous glucose (7-10 mg/kg of weight per min), oral Ensure (7-10 mg of carbohydrate/kg of weight per min), or a high-carbohydrate mixed-meal breakfast (3.5 g of carbohydrate/kg of weight). Respiratory quotients remained , 1.0. High-performance liquid ...
The sotalol enantiomers produce different plant preparations for according to mctaboliteantimetubolitc concepts. The angioten- thyroxmneprealbumin complex, the principal one being hydroxylatcd in the target enzyme for the attachment to microbe, cell surface where hormones such as the ones who have when prescribing the most important and sustained for duration of action is probably indigenous to bangladesh, india, and hospital-based data had across different european countries. The difference between the cellular infections (such as diabetes). A variation of the skin. Radiopharmaceutical preparations 01/2010:175. Is a dihydropyridine lymphocytes and other cardiovascular disease continuum. Arch ci catechol hydroxyls terol. Fraudulent data could jeopardise patient safety agency (npsa) (2005) promoting safer use this agent.365 to yield several types of subscituenis to test the influence of western religion and education, urbanization and globalization phenom- ena in africa traditional medicine for ...
first mention what is polyacrylamid gel, where are used (e.g used genetic lab, hydraulic fracturing as friction agent, agriculture,etc,,), then describe how industry think it is safe and not consider a hazard, then describe the factors in environment that can lead to the degradation of polyaccrylamide gel from any kind of industry after that you will mention the health concern when this polyacrylamide gel in different industry can degraded to a toxic form which is acrylamide monomer, then describe the toxicity of the monomer, add many as you can find about accidental leaking or release of the polymer or it is monomer to environment and cause adverse effect to human and animals (e.g: the grout used in tunnel building cause sever illness to the labor because it has poly acrylamid gell in Sweden), find accident happened in hydraulic fracturing area all these introduction , now you will start the hypothetic question that How can we discriminate and track the origin of contamination , is the ...
^12C. ^13C, and ^14C. The heaviest isotope shown has a half life of about 6000 years (this is from memory, so check). The ^13C isotope is abundant enough (approx. 1%) to make ^13C NMR spectroscopy a routine method of characterization for organic chemists. Most universities would have 1 or more ^13C NMR spectrometers. ^12C isotope is the bog standard, naturally abundant one that you sprinkle on your cornflakes when it (the isotope) is bound to oxygen and hydrogen.
SHIRLEY - Sep 30th, 2017 - Creative Proteomics, which specializes in a full range of services to support various proteome-related researches from identification of single proteins to large-scale proteomic studies. Besides, we also offer professional products to support further researches and studies. Such as the Stable Isotope Standard, that will be helpful for the proteomics research use.. Stable isotope labeling with amino acids is the new technology that is used for halo analysis of protein expression by taking advantage of stable isotope labeling amino acid binding mass spectrometry in the cell culture process. It can provide support for qualitative analysis of the protein, also offer accurate quantitative analysis with fewer sample requirements, but the simple and efficient process.. The applications and specific strategies of SILAC technology are in the continuous development of updates. There are many applications and improvements, such as the differential expression of proteins, ...
These elements have several common characteristics: 1)They have low atomic mass; 2)The relative mass difference between the isotopes is large; 3)They form bonds with a high degree of covalent character; 4)They exist in more than one oxidation state, form a wide variety of compounds; 5)The abundance of the rare isotope is sufficiently high (at least tenths of a percent) to facilitate analysis. Terrestrial Abundance of Stable Isotopes: ElementIsotopeAbundance % Hydrogen 1 H H0.015 Carbon 12 C C1.11 Nitrogen 14 N N0.37 Oxygen 16 O O O0.204 Sulfur 32 S S S S0.014
2011:. Mester G, Hoffmann V, Stevanovic S (2011) Insights into MHC class I antigen processing gained from large-scale analysis of class I ligands. Cell Mol Life Sci 68:1521-1532 2009: Klug F, Miller M, Schmidt HH, Stevanovic S (2009) Characterization of MHC ligands for peptide based tumor vaccination. Curr Pharm Des 15:3221-3236 2007: Schuler MM, Nastke MD, Stevanovic S (2007) SYFPEITHI: Database for searching and T cell epitope prediction. Methods Mol Biol 409:75-93 Gouttefangeas C, Stenzl A, Stevanovic S, Rammensee HG (2007) Immunotherapy of renal cell carcinoma. Cancer Immunol Immunother 56:117-128 2006: Weinzierl AO, Stevanovic S (2006) LC-MS-based protein and peptide quantification using stable isotope labels. Biotechnol Gen E Rev 23:21-39 Dengjel J, Stevanovic S (2006) Naturally presented MHC ligands carrying glycans. Transfus Med Hemother 33:38-44 Hillen N, Stevanovic S (2006) Contribution of mass spectrometry-based proteomics to immunology. Expert Rev Proteomics 3:653-664 Schmid D, ...
2011:. Mester G, Hoffmann V, Stevanovic S (2011) Insights into MHC class I antigen processing gained from large-scale analysis of class I ligands. Cell Mol Life Sci 68:1521-1532 2009: Klug F, Miller M, Schmidt HH, Stevanovic S (2009) Characterization of MHC ligands for peptide based tumor vaccination. Curr Pharm Des 15:3221-3236 2007: Schuler MM, Nastke MD, Stevanovic S (2007) SYFPEITHI: Database for searching and T cell epitope prediction. Methods Mol Biol 409:75-93 Gouttefangeas C, Stenzl A, Stevanovic S, Rammensee HG (2007) Immunotherapy of renal cell carcinoma. Cancer Immunol Immunother 56:117-128 2006: Weinzierl AO, Stevanovic S (2006) LC-MS-based protein and peptide quantification using stable isotope labels. Biotechnol Gen E Rev 23:21-39 Dengjel J, Stevanovic S (2006) Naturally presented MHC ligands carrying glycans. Transfus Med Hemother 33:38-44 Hillen N, Stevanovic S (2006) Contribution of mass spectrometry-based proteomics to immunology. Expert Rev Proteomics 3:653-664 Schmid D, ...
Positron emission tomography (PET) is a non-invasive molecular imaging technique, which relies on the availability of radiolabelled molecular probes for molecular-level diagnostics, biological research and drug discovery.1 Worldwide, cyclotron sites are in place for the production of radioisotopes and selected labelled biomarkers, but to date, rapid progress in PET imaging is limited by the cost, speed, and efficiency of synthetic methods to access structurally diverse radiolabelled probes. Ideally, the radiosynthetic protocol should be as efficient as possible to minimise functional manipulation after introduction of radioisotopes and to avoid the formation of side-products that cause complications during purification.218F is commonly identified as the radionuclide of choice due, in part, to its relatively long half-life of 110 min which allows for multistep radiosynthetic protocols. In addition, the low emitted positron energy of fluorine-18 can allow for higher resolution PET images.3 Direct ...
Cambridge Isotope Laboratories offers a complete listing of Stable Isotope Labeled Deuterium Bromide for all your research needs. View pricing, availability and product specifications.
University of Waikato RNA based stable isotope probing (SIP) facilitates the detection and identification of active members of microbial populations that are involved in the assimilation of an isotopically labeled compound. ¹⁵N-RNA-SIP is a new method that has been discussed in recent literature but has not yet been tested. Herein, we define the limitations to using ¹⁵N-labeled substrates for SIP and propose modifications to compensate for some of these shortcomings. We have used ¹⁵N-RNA-SIP as a tool for analysing mixed bacterial populations that use nitrogen substrates. After incubating mixed microbial communities with ¹⁵N-ammonium chloride or ¹⁵N₂ we assessed the fractionation resolution of ¹⁵N-RNA by isopycnic centrifugation in caesium trifluoroacetate (CsTFA) gradients. We found that the more isotopic label incorporated, the further the buoyant density (BD) separation between ¹⁵N- and ¹⁴N-RNA, however it was not possible to resolve the labeled from unlabeled RNA ...
Protein quantification without isotopic labels has been a long-standing interest in the proteomics field. However, accurate and robust proteome-wide quantification with label-free approaches remains a challenge. We developed a new intensity determination and normalization procedure called MaxLFQ that is fully compatible with any peptide or protein separation prior to LC-MS analysis. Protein abundance profiles are assembled using the maximum possible information from MS signals, given that the presence of quantifiable peptides varies from sample to sample. For a benchmark dataset with two proteomes mixed at known ratios, we accurately detected the mixing ratio over the entire protein expression range, with greater precision for abundant proteins. The significance of individual label-free quantifications was obtained via a t test approach. For a second benchmark dataset, we accurately quantify fold changes over several orders of magnitude, a task that is challenging with label-based methods. ...
ANXA10 Fragment MS Protein Standard, is a protein fragment containing a 50-150 amino acid sequence identical to part of a human ANXA10 protein target. The fragment MS Protein Standard represents a new category of using heavy isotope labeled (15N, 13C) Lysine and Arginine residues resulting in more than 99% isotope incorporation, as internal MS standards offering distinct advantages to existing products for relative and absolute quantification.
Isotopic distribution is a function of physical and biological processes. In general, equilibrium-controlled reactions occur at high temperatures among solid phases, whereas low-temperature reactions, especially those mediated by organisms, are kinetically controlled. The solar and stellar abundance of isotopes is poorly known. Knowledge of extra-terrestrial distributions of the isotopes of light elements presently comes from lunar and meteorite measurements. On Earth, the elements associated with biological synthesis which have been studied most intensively, are hydrogen, carbon, nitrogen, oxygen and sulphur. In general, reduced products of metabolism are enriched in light isotopes. Thus, 1H and 12C are enriched in hydrogen gas and methane, when produced by fermentation or CO2 reduction. Nitrogen gas is enriched in 14N when produced by denitrification, and H2S is highly enriched in 32S when it results from sulphate reduction. A pattern of biological enrichment factors has been recognized on ...
EMR2 Fragment MS Protein Standard, is a protein fragment containing a 50-150 amino acid sequence identical to part of a human EMR2 protein target. The fragment MS Protein Standard represents a new category of using heavy isotope labeled (15N, 13C) Lysine and Arginine residues resulting in more than 99% isotope incorporation, as internal MS standards offering distinct advantages to existing products for relative and absolute quantification.
Isotope labeling[edit]. Main article: Mass cytometry. Mass cytometry overcomes the fluorescent labeling limit by utilizing ... Fluorescent labels[edit]. Main article: Fluorophore. A wide range of fluorophores can be used as labels in flow cytometry.[10] ... This method could theoretically allow the use of 40 to 60 distinguishable labels and has been demonstrated for 30 labels.[30] ... The label-free technology has recently been enhanced by a "lab-on-a-chip" based approach and by applying high frequency ...
... (Abkürzung von stable isotope labeling by/with amino acids in cell culture) ist eine massenspektrometrische Methode zur ... Stable isotope labeling by amino acids in cell culture, SILAC, as a simple and accurate approach to expression proteomics. . In ... Stable isotope labeling by amino acids in cell culture for quantitative proteomics. . In: Methods in Molecular Biology. . 359, ... A practical recipe for stable isotope labeling by amino acids in cell culture (SILAC). . In: Nature Protocols. . 1, Nr. 6, 2006 ...
After labeling the ribosomes of a laboratory strain of Escherichia coli with radioactive isotopes, they followed bacterial ... When the researchers added isotope-labeled arsenate to the solution to track its distribution, they found that arsenic was ... A comparable control experiment with isotope-labeled phosphate was not performed. With the distribution of the strain in mid- ... it is often labeled as the genus name followed by "sp." (i.e., undetermined species of that genus) and the strain name. In the ...
Some isotope ratios are used to determine the age of materials for example as in carbon dating. Labeling with stable isotopes ... Isotope ratio MS: isotope dating and tracing[edit]. Mass spectrometer to determine the 16O/18O and 12C/13C isotope ratio on ... 10.1 Isotope ratio MS: isotope dating and tracing *10.1.1 Membrane-introduction mass spectrometry: measuring gases in solution ... Differences in mass among isotopes of an element are very small, and the less abundant isotopes of an element are typically ...
A relative quantitation can be obtained with a variety of differential isotope labeling technologies.[47] There are also ... "Proteome analysis of low-abundance proteins using multidimensional chromatography and isotope-coded affinity tags". J. Proteome ...
Multiplexed ion beam imaging (MIBI) is a SIMS method that uses metal isotope labeled antibodies to label compounds in ...
... and 13C-isotope labeled 40-residue human Alzheimer's β-amyloid peptide for NMR-based structural analysis". Protein Expression ... Vargo MA, Colman RF (January 2001). "Affinity labeling of rat glutathione S-transferase isozyme 1-1 by 17beta -iodoacetoxy- ...
Furthermore, labeling compounds with micro-SPECT isotopes require chelating molarities which may alter their biochemical or ... Weaknesses: Radioactive isotopes used in micro-PET have very short half-lives (110 min for 18F-FDG). In order to generate these ... Since newer radioisotopes involve nanoparticles such as 99mTC-labelled iron oxide nanoparticles, they could potentially be ... and have longer half-lives as compared to micro-PET isotopes. Like micro-PET, micro-SPECT also has very good sensitivity and ...
This was first demonstrated by specifically labelling replicating bacterial chromosomes with radioactive isotopes. The regions ... See Figure 4 of D. M. Prescott, and P. L. Kuempel (1972): A grain track produced by an E. coli chromosome from cells labeled ... In his experiment, he radioactively labeled the chromosome by growing his cultures in a medium containing 3H-thymidine. The ...
Some isotope ratios are used to determine the age of materials for example as in carbon dating. Labeling with stable isotopes ... Isotope ratio MS: isotope dating and tracingEdit. Mass spectrometer to determine the 16O/18O and 12C/13C isotope ratio on ... Differences in mass among isotopes of an element are very small, and the less abundant isotopes of an element are typically ... 1015 relative to the major stable isotope.[41] Isotope ratios are important markers of a variety of processes. ...
Sulesomab, an antigen that recognizes proteins on the surface of granulocytes, is used to label out infections, again using the ... 99mTc isotope.[6] Fab fragments are often fused to small proteins (,100 kDa) that have lower scattering, resulting in images ... It is conjugated to a radioactive element, which will label the tumors when viewed with single photon emission computed ...
"Stable isotope labeling with amino acids in cell culture" (SILAC). 13C-enriched compounds are used in medical diagnostic tests ... Carbon-13 (13C) is a natural, stable isotope of carbon with a nucleus containing six protons and seven neutrons. As one of the ... Because the different isotope ratios for the two kinds of plants propagate through the food chain, it is possible to determine ... Analysis in these tests is usually of the ratio of 13C to 12C by isotope ratio mass spectrometry. ...
"Cell-free synthesis and amino acid-selective stable isotope labeling of proteins for NMR analysis". Journal of Biomolecular NMR ... were able to successfully label amino acids in a cell-free system where amino acid metabolism was no longer present, thus ... such as the process of amino acid metabolism preventing specific labelling of amino acids that would be useful in ...
Isotope labeling and kinetics experiments suggest that the mechanism involves PdCH2CH2OAc-containing intermediates. Beta- ...
Typically, the first experiment to be measured with an isotope-labelled protein is a 2D heteronuclear single quantum ... requires isotope labeling of the protein. The T1 and T2 relaxation times can be measured using various types of HSQC-based ... but this has been alleviated by the introduction of isotope labelling and multidimensional experiments. Another more serious ... In NMR studies of protein dynamics the nitrogen-15 isotope is the preferred nucleus to study because its relaxation times are ...
... are incorporated from the molecular oxygen while in the second step only one is incorporated as determined by isotope labeling ...
"Insulin-dependent interactions of proteins with GLUT4 revealed through stable isotope labeling by amino acids in cell culture ( ...
Autoradiography, also used for protein band detection post gel electrophoresis, uses radioactive isotopes to label proteins, ...
... stable isotope labeling and mass spectrometry (англ.) // Nature Biotechnology : journal. - Nature Publishing Group, 2003. - Vol ...
Site-specific isotope enrichment must be done through chemical synthesis of the labeled nucleoside phosphoramidite monomer and ... Kojima, C; Ono, A; Ono, A; Kainosho, M (2002). "Solid-phase synthesis of selectively labeled DNA: Applications for ... nucleotide-type selective labeling of RNA and usage of heteronuclear correlation experiments. ...
When labeled with the isotope fluorine-18 it is used as a radioligand in positron emission tomography (PET) studies of the ... Alternatively, the voltage swings are labeled based on their order, N1 being the first negative swing observed, N2 the second ...
The 131I isotope is also used as a radioactive label for certain radiopharmaceuticals that can be used for therapy, e.g. 131I- ... see isotopes of iodine) are preferred in situations when only nuclear imaging is required. The isotope 131I is still ... Isotopes of iodine Complete table of nuclides. Iodine-131 (131I, I-131) is an important radioisotope of iodine discovered by ... "In Aggarwal, Pradeep K.; Gat, Joel R.; Froehlich, Klaus F. (eds.). Isotopes in the water cycle : past, present and future of a ...
2-hydroxyglutarate is likely a terminal metabolite as isotope labelling experiments of colorectal cancer cell lines show that ... Wolfe RR, Jahoor F (February 1990). "Recovery of labeled CO2 during the infusion of C-1- vs C-2-labeled acetate: implications ...
Isotope ratios (e.g.,13 C/12 C, 15 N/14 N) can reveal history in the absence of any label. Because kinetic isotope effects ... 13C-labeled parent pesticides were used in nontarget analysis of degraders by stable isotope probing (SIP) to demonstrate ... heavy isotopes (13C) become enriched in residues. An increased 13 C/12 C isotope ratio in a parent compound thus provides ... and nitrogen-isotope effects. The approach requires a relatively high amount of substance for gas chromatography-isotope ratio ...
The NIH-shift, which has been demonstrated through isotope-labeling studies, involves migration of an alkyl group to form a ...
This mechanism is supported by isotope labeling experiments. For example, when ethyl propionate with an oxygen-18 labeled ...
This mechanism is supported by isotope labeling experiments. When ethyl propionate with an oxygen-18-labeled ethoxy group is ... treated with sodium hydroxide (NaOH), the oxygen-18 label is completely absent from propionic acid and is found exclusively in ...
... unlike compounds with labelled hydrogen, carbon, and oxygen isotopes that must be kept away from the atmosphere.[19] The 15N:14 ... The heavy isotope 15N was first discovered by S. M. Naudé in 1929, soon after heavy isotopes of the neighbouring elements ... Iso-tope Abun-dance Half-life (t1/2) Decay mode Pro-duct ... No other nitrogen isotopes are possible as they would fall ... Of the ten other isotopes produced synthetically, ranging from 12N to 23N, 13N has a half-life of ten minutes and the remaining ...
"Multiplex peptide stable isotope dimethyl labeling for quantitative proteomics". Nature Protocols. 4 (4): 484-494. doi:10.1038/ ... "Metabolic labeling of C. Elegans and D. Melanogaster for quantitative proteomics". Nature Biotechnology. 21 (8): 927-931. doi: ...
Support for the reaction mechanism is based on isotope labeling. The reaction between menthone ((2S,5R)-2-isopropyl-5- ... is replaced by deuterium for isotopic labeling. Reaction conditions: 90°C, 24 hrs. 80% chemical yield. The first step is ...
18F-labeled 2-deoxy-2-fluoro-D-glucose, 2-deoxy-2-fluoro-D-mannose and 14C-2-deoxy-2-fluoro-D-glucose". Journal of Labelled ... A few other isotopes and radiotracers are slowly being introduced into oncology for specific purposes. For example, 11C- ... Such labelled compounds are known as radiotracers. PET technology can be used to trace the biologic pathway of any compound in ... Radionuclides used in PET scanning are typically isotopes with short half-lives[3] such as carbon-11 (~20 min), nitrogen-13 (~ ...
Future developments led to the isotope-power source that would last for the lifespan of the patient.[page needed] ... Class II devices are subject to special labeling requirements, mandatory performance standards and postmarket surveillance.[18] ...
Primordial isotopes of the alkali metals Z. Alkali metal. Stable. Decays. unstable: italics. odd-odd isotopes coloured pink ... group VIII was labelled a transition group) to lack an A-B bifurcation.[29] Soon afterward, a majority of chemists chose to ... No primordial isotopes. (223. Fr. is a radiogenic nuclide) Radioactive: 40K, t1/2 1.25 × 109 years; 87Rb, t1/2 4.9 × 1010 years ... Francium-223, the only naturally occurring isotope of francium,[58][59] is the product of the alpha decay of actinium-227 and ...
Researchers in the field do not agree on a theory for cold fusion.[126] One proposal considers that hydrogen and its isotopes ... label continues to serve a social function in creating a collective identity for the field.[77] ... This creates a high partial pressure, reducing the average separation of hydrogen isotopes. However, the reduction in ... the possible existence of fusion involving hydrogen isotopes in a planetary core.[24] In his original paper on this subject ...
Melville, G; Allen, Bj (2009). "Cyclotron and linac production of Ac-225". Applied Radiation and Isotopes. 67 (4): 549-55. doi: ... 2004). "Alpha-particle emitting atomic generator (Actinium-225)-labeled trastuzumab (herceptin) targeting of breast cancer ... Applied Radiation and Isotopes. 62 (5): 667-679. doi:10.1016/j.apradiso.2004.12.003. PMID 15763472.. ...
Popular culture and a growing number[citation needed] of scientists use the term "Anthropocene" informally to label the current ... Earliest evidence for life based on unusually high amounts of light isotopes of carbon, a common sign of life. 4280[36]. ...
If you have just labeled this page as a potential copyright issue, please follow the instructions for filing at the bottom of ... For example, rubidium chloride is used as a radioactive isotope to evaluate perfusion of heart muscle. ...
In the late 1960s, several companies, including ARCO in the USA, developed isotope-powered pacemakers, but this development was ... which was the first to be labeled as conditional[15] for MRI use.[16] There are several limitations to its use including ...
Although Sagan has been identified as an atheist according to some definitions,[294][295][296] he rejected the label, stating " ... Harold Urey (1893-1981): American physical chemist whose pioneering work on isotopes earned him the Nobel Prize in Chemistry in ... I ended up being one of two panelists labeled 'atheists.'..." God and Science Don't Mix: A scientist can be a believer. But ... adding that an orthodoxy which forbids acknowledgement of the possibility of change is as flawed as that which labels ...
Clayton, Donald D. (2003). Handbook of Isotopes in the Cosmos: Hydrogen to Gallium. Cambridge University Press. ISBN 0521823811 ... "1H NMR studies of deuterated ribonuclease HI selectively labeled with protonated amino acids". Journal of Biomolecular NMR. 2 ... B. (2004). "Spectroscopy of superheavy hydrogen isotopes in stopped-pion absorption by nuclei". Physics of Atomic Nuclei. 68 (3 ... Kendall, Carol (1998). "Fundamentals of Isotope Geochemistry". US Geological Survey. Diakses pada 8 Maret 2008. ...
Isotopes commonly used in the treatment of bone metastasis are radium-223,[73] strontium-89 and samarium (153Sm) lexidronam.[74 ... which is a combination of an iodine-131 labelled and an unlabelled anti-CD20 monoclonal antibody.[76] These medications were ... Targeting can be due to the chemical properties of the isotope such as radioiodine which is specifically absorbed by the ...
Detection of labelled biomolecules[edit]. Cherenkov radiation is widely used to facilitate the detection of small amounts and ... and isotopes 18F and 131I have been imaged in humans for diagnostic value demonstration.[15][16] External beam radiation ...
Various cycles in those isotope ratios have been detected.. *Pollen has been observed in the ice cores and can be used to ... Sea floor core sample labelled to identify the exact spot on the sea floor where the sample was taken. Sediments from nearby ... The early basic carbon isotopy (isotope ratio proportions) was very much in line with what is found today, suggesting that the ... containing the heavier O-18 evaporate at a higher temperature than water molecules containing the normal Oxygen-16 isotope. The ...
Maslin, Mark A.; Swann, George E.A. (2006). "Isotopes in marine sediments". In Leng, Melanie J. Isotopes in Palaeoenvironmental ... Cotton wool, cigarette ash, paper labels, cloth bags, and some conservation chemicals such as polyvinyl acetate can all be ... The differential uptake of the three carbon isotopes leads to 13. C/12. C and 14. C/12. C ratios in plants that differ from the ... C is proportional to the difference in the atomic masses of the two isotopes, so once the δ13C value is known, the depletion ...
... confusingly labelled q. _. _. {\displaystyle \scriptstyle {\underline {\underline {q}}}}. , another rank-2 tensor given by the ... Typically, the hyperfine structure transition frequency of a particular isotope of caesium or rubidium atoms is used as a basis ... The atomic vapor laser isotope separation (AVLIS) process uses the hyperfine splitting between optical transitions in uranium- ...
IsotopesEdit. Main article: Isotopes of ytterbium. Natural ytterbium is composed of seven stable isotopes: 168Yb, 170Yb, 171Yb ... Ytterbium has three allotropes labeled by the Greek letters alpha, beta and gamma; their transformation temperatures are −13 °C ... The 169Yb isotope (with a half-life of 32 days), which is created along with the short-lived 175Yb isotope (half-life 4.2 days ... The primary decay mode of ytterbium isotopes lighter than the most abundant stable isotope, 174Yb, is electron capture, and the ...
There was limited Neanderthal occupation of Britain in marine isotope stage 3 between about 60,000 and 42,000 years BP. Britain ... still use the term to refer to the pre-Roman inhabitants of Britain for want of a better label. ...
The labels of the traditional "Big Five" extinction events and the more recently recognised End-Capitanian extinction event are ... The team analyzed combined extinction patterns using a new clumped isotope temperature record from a hiatus-free, expanded K-Pg ...
Alpharadin uses bone targeted Radium-223 isotopes to kill cancer cells by alpha radiation.[183][unreliable medical source?] It ... a randomised open-label trial". Lancet. 376 (9747): 1147-54. doi:10.1016/S0140-6736(10)61389-X. PMID 20888992.. ...
"Dirty work: Soil formation and isotope fractionation behind Brumbaugh Academic Center" (PDF). Juniata College Geology ... The Tioga ash bed zone consists of eight ash beds labeled according to their stratigraphic order from A (oldest) to H (youngest ...
Several of the essays form a sequence explaining the discovery and uses of isotopes. ... "Labels on the Molecules" (July 1986). *"The Consequences of Pie" (August 1986) ...
Isotope Development & Production for Research and Applications (IDPRA) U.S. Department of Energy program for isotope production ... in vitro labelled leucocytes. 18. 360. 60. 64 x 64. 30 SPECT/CTEdit. In some cases a SPECT gamma scanner may be built to ... On occasion, the radioisotope is a simple soluble dissolved ion, such as an isotope of gallium(III). Most of the time, though, ... National Isotope Development Center Reference information on radioisotopes including those for SPECT; coordination and ...
... feeding studies with carbon isotope-labeled amino acids or related molecules in pure cultures of the loline-producing fungus N ...
... indicating incorporation of the carbon isotopes. This enrichment was consistent with two biosynthetic pathways. The labeling ... In 1999, using 13C- and 14C-labelled precursors, the biosynthesis of domoic acid in the diatom genus Pseudo-nitzschia was ...
A 2013 study by Oceana found that one third of seafood sampled from the United States was incorrectly labelled. Snapper and ... "Stable isotope dietary analysis of the Tianyuan 1 early modern human". Proceedings of the National Academy of Sciences. 106 ...
Isotopes of carbon Complete table of nuclides. There are three naturally occurring isotopes of carbon on Earth: carbon-12, ... In the initial variant of the urea breath test, a diagnostic test for Helicobacter pylori, urea labeled with approximately 37 ... Carbon-14 (14C), or radiocarbon, is a radioactive isotope of carbon with an atomic nucleus containing 6 protons and 8 neutrons ... "Class notes for Isotope Hydrology EESC W 4886: Radiocarbon 14C". Martin Stute's homepage at Columbia. Archived from the ...
... they are ideally prepared in isotope labeled form. In this chapter different strategies for isotope labeling in insect cells ... Isotope labeling in insect cells Adv Exp Med Biol. 2012;992:179-96. doi: 10.1007/978-94-007-4954-2_10. ... Although E. coli is the organism of choice for expression of proteins in isotope labeled form for NMR studies, bacterial cells ... The chapter is concluded with case studies, describing successful application of isotope labeling in insect cells for NMR ...
Amino acid residue specific stable isotope labeling for quantitative proteomics.. Zhu H1, Pan S, Gu S, Bradbury EM, Chen X. ... Various stable isotope labeling (SIL) techniques have recently emerged to improve the efficiency and accuracy of protein ... For proteins whose expression levels are the same in cells grown in the normal and labeled media, the relative areas of the ... This strategy is generally applicable using any particular type of amino acid as the labeling precursors for accurate ...
One of the major advancements in NMR spectroscopy of biomolecules has been the development of methods for isotope labeling. At ... Isotope Labeling and Segmental Labeling of Larger RNAs for NMR Structural Studies ... Stereo-Array Isotope Labeling Method for Studying Protein Structure and Dynamics Yohei Miyanoiri, Mitsuhiro Takeda, Masatsune ... At the same time there has been a rapid progress in the number of NMR experiments that utilize such isotope labeled samples. ...
Ion of m/z 1100.6 is the product with the light isotope tag, and ion m/z 1106.5 is the product with the heavy isotope tag. The ... Quantitative analysis of snake venoms using soluble polymer-based isotope labeling.. Galan JA1, Guo M, Sanchez EE, Cantu E, ... We present the design and synthesis of a new quantitative strategy termed soluble polymer-based isotope labeling (SoPIL) and ... Quantitative Analysis of Snake Venoms Using Soluble Polymer-based Isotope Labeling. Mol Cell Proteomics. 2008 Apr;7(4):785-799. ...
We discuss recent advances in inexpensive isotope-labeling methods using labeled algal or yeast extracts as the amino acid ... An economic approach to efficient isotope labeling in insect cells using homemade 15N-, 13C- and 2H-labeled yeast extracts. J ... Saxena K, Dutta A, Klein-Seetharaman J, Schwalbe H (2012) Isotope labeling in insect cells. Methods Mol Biol 831:37-54CrossRef ... Strauss A, Bitsch F, Fendrich G, Graff P, Knecht R, Meyhack B, Jahnke W (2005) Efficient uniform isotope labeling of Abl kinase ...
Buy the Hardcover Book Isotope Labeling Of Biomolecules Applications by Zvi Kelman at Indigo.ca, Canadas largest bookstore. + ... Isotope Labeling of Biomolecules: Applications,the latest in theMethods in Enzymologyseries, focuses on stable isotope labeling ... Isotope Labeling Of Biomolecules Applications. byZvi KelmanEditorZvi Kelman. Hardcover , January 12, 2016. ... Title:Isotope Labeling Of Biomolecules ApplicationsFormat:HardcoverDimensions:482 pages, 9.41 × 7.24 × 0.98 inPublished:January ...
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Stable Isotope Labeling by/with Amino acids in Cell culture (SILAC) is a technique based on mass spectrometry that detects ... Ong SE, Blagoev B, Kratchmarova I, Kristensen DB, Steen H, Pandey A, Mann M (May 2002). "Stable isotope labeling by amino acids ... Zhu H, Pan S, Gu S, Bradbury EM, Chen X (2002). "Amino acid residue specific stable isotope labeling for quantitative ... Amanchy R, Kalume DE, Pandey A (January 2005). "Stable isotope labeling with amino acids in cell culture (SILAC) for studying ...
The combination of 15N/13C stable isotope labelling (SIL) and LC-MS/MS revealed a total of 52 modifications in RNA from E. coli ... Profiling of RNA modifications by multiplexed stable isotope labelling Stefanie Kellner,a Jennifer Neumann,a David Rosenkranz,b ... The combination of 15N/13C stable isotope labelling (SIL) and LC-MS/MS revealed a total of 52 modifications in RNA from E. coli ... Profiling of RNA modifications by multiplexed stable isotope labelling S. Kellner, J. Neumann, D. Rosenkranz, S. Lebedeva, R. F ...
Investigating Optimal Propionate Delivery to the Colon Using Stable Isotope Labeling. The safety and scientific validity of ... We plan to use non-invasive, stable isotope labelling methodologies to determine propionate bioavailability from a range of ... Isotope and appetite measurements on day 7.. Dietary Supplement: 2.5 g of propionate This system delivers approximately 2.5 g ... Isotope and appetite measurements on day 7.. Dietary Supplement: 5 g of propionate This delivers approximately 5g of propionate ...
Here, we developed a strategy by combining stable isotope labeling (IL) with liquid chromatography-mass spectrometry (LC-MS) ... All the MRM transitions of light and heavy labeled thiols were acquired from urines by using DPI method. Compared to DPI method ... In the forward labeling, the cancer sample was labeled with BQB and the healthy control sample was labeled by BQB-d7. In the ... the cancer sample was labeled with BQB-d7 and the healthy control sample was labeled by BQB. Then the two labeled samples were ...
MALDI Biotyper-Based Rapid Resistance Detection by Stable-Isotope Labeling. Katrin Sparbier, Christoph Lange, Jette Jung, ... MALDI Biotyper-Based Rapid Resistance Detection by Stable-Isotope Labeling. Katrin Sparbier, Christoph Lange, Jette Jung, ... SILAC (stable isotope labeling by/with amino acids in cell culture) is a common technique for quantitative proteomics (12). ... MALDI Biotyper-Based Rapid Resistance Detection by Stable-Isotope Labeling. Katrin Sparbier, Christoph Lange, Jette Jung, ...
... uses amino acids to label cultured cells for quantitative proteomics. Learn more. ... Stable Isotope Labeling with Amino Acids in Cell Culture, or SILAC ... Stable isotope labeling by amino acids in cell culture, SILAC, as a simple and accurate approach to expression proteomics. Mol ... Stable Isotope Labeling with Amino Acids in Cell Culture (SILAC). Akhilesh Pandey, PhD. Johns Hopkins University ...
non- linear Scatchard plot using radio isotope labeled ligand. - (May/18/2016 ). How to calculate non- linear Scatchard plot ... using adhered cell culture and radio isotope labeled ligand. I am studying the cooperativity in EGFR . ...
This protocol describes the reconstitution of nucleosomes containing differentially isotope-labeled sister histones. At the ... C) 1H-15N SOFAST-HMQC spectrum of the 15N-labeled H3 copy of differentially isotope-labeled nucleosomes (only H3 tail residues ... 13C HSQC spectrum of the 13C-labeled H3 copy of differentially isotope-labeled nucleosomes. Please click here to view a larger ... These pools are differentially isotope-labeled (one with 15N, the other with 13C), and they are fused to a polyhistidine and a ...
MAb Trastuzumab Stable-Isotope Labeled Monoclonal Antibody recombinant, expressed in CHO cells; find Sigma-Aldrich-MSQC23 MSDS ... SILu™MAb Trastuzumab Stable-Isotope Labeled Monoclonal Antibody recombinant, expressed in CHO cells ... SILu™MAb Trastuzumab (MSQC23) is a recombinant, stable isotope-labeled, monoclonal antibody which incorporates [13C6, 15N4]- ... Lot and Batch Numbers can be found on a products label following the words Lot or Batch. ...
Stable isotope labeling by amino acids in cell culture, SILAC, as a simple and accurate approach to expression proteomics ... However, many stable isotope label approaches rely on labeling in vitro using complex and sometimes expensive reagents. This ... However, many stable isotope label approaches rely on labeling in vitro using complex and sometimes expensive reagents. This ... Stable isotope labeling by amino acids in cell culture, SILAC, as a simple and accurate approach to expression proteomics (2002 ...
Stable isotope labelled compounds, Research Tools at LGC Standards. Over 100,000 Products Online, Explore our Extensive Range ... Stable isotope labelled compounds. Stable isotope labelled compounds. Common stable isotopes used in research include 2H ( ... Custom isotope labelling. We offer off-the-shelf pricing for all our high-quality custom stable isotope labelling products & ... Pharmaceutical stable isotopes Explore our extensive range of isotopically labelled products for your pharmaceutical research, ...
Here, we report metabolic isotope labeling to produce isotopically labeled internal standards in bacteria and yeast. These can ... or by metabolic labeling [14]. In many fields, metabolic isotope labeling for production of stable isotope-labeled internal ... For absolute quantification, isotope dilution mass spectrometry is the method of choice and thus stable isotope labeled ... The mass spectra of the heavy isotope labeled RNA digests showed a high labeling efficiency for both organisms. We used ...
MAb Mouse Stable-Isotope Labeled Monoclonal Antibody from mouse, recombinant, expressed in CHO cells; find Sigma-Aldrich-MSQC10 ... Produced utilizing enriched media containing stable isotope labeled amino acids such as [13C6, 15N4]-Arginine and [13C6, 15N2]- ... SILu™MAb Mouse Stable-Isotope Labeled Monoclonal Antibody from mouse, recombinant, expressed in CHO cells ... SILu™MAb Mouse is a recombinant, stable isotope-labeled, mouse monoclonal antibody which incorporates [13C6, 15N4]-Arginine and ...
The deuterium-labelled and the unlabelled form of the drug to be investigated were simultaneously infu ... The pharmacokinetics of lidocaine and bupivacaine and tri-deuteromethyl-labelled lidocaine and bupivacaine were investigated in ... 3342287 - Pharmacokinetics of lidocaine and bupivacaine and stable isotope labelled analogues: a .... 8420907 - Anesthetic ... deuterium-labelled lidocaine 39 +/- 161 and 98 +/- 181, bupivacaine 27 +/- 111 and 66 +/- 231 and deuterium-labelled ...
Stable isotope labeling by essential nutrients in cell culture (SILEC) for accurate measurement of nicotinamide adenine ... Stable isotope labeling by essential nutrients in cell culture (SILEC) for accurate measurement of nicotinamide adenine ... and provides a simple method for generating a library of stable isotope labeled internal standards for quantifying and tracing ... Using a two-label system incorporating [13C315N1]-nicotinamide and [13C11]-tryptophan, we quantify the relative contribution of ...
Solution structure of stereo-array isotope labelled (SAIL) C-terminal dimerization domain of SARS coronavirus nucleocapsid ... We have recently developed the stereo-array isotope labeling (SAIL) method to overcome the size problem of NMR structure ... Solution structure of stereo-array isotope labelled (SAIL) C-terminal dimerization domain of SARS coronavirus nucleocapsid ... and regio-specifically isotope-labeled amino acids. Here, we employed the SAIL method to determine the high-quality solution ...
... a method by which 15N-labeled nitrotyrosine groups are generated on peptide or protein standards using stable isotope-labeled ... Evaluation of a Method for Nitrotyrosine Site Identification and Relative Quantitation Using a Stable Isotope-Labeled Nitrated ... Evaluation of a Method for Nitrotyrosine Site Identification and Relative Quantitation Using a Stable Isotope-Labeled Nitrated ... "Evaluation of a Method for Nitrotyrosine Site Identification and Relative Quantitation Using a Stable Isotope-Labeled Nitrated ...
Solution structure of BCL-xL containing the alpha1-alpha2 disordered loop determined with selective isotope labelling of I,L,V ... Solution structure of BCL-xL containing the alpha1-alpha2 disordered loop determined with selective isotope labelling of I,L,V ...
... more about teh collaboration between CIl and researchers at North Carolina State University to develop a new 13C3/15N2 labeled ... Stable Isotope Newsletters , Cambridge Isotope Laboratories stable isotope, stable isotope labeled compounds, environmental ... 2. Analyzing Cyanotoxins Using LC-MS/MS with 15N-Stable-Isotope Labeled Internal Standards. Cyanotoxins are toxic bioactive ... 2. Analyzing Cyanotoxins Using LC-MS/MS with 15N-Stable-Isotope Labeled Internal Standards ...
... mass spectrometric methods based on stable isotope quantitation have shown great promise for the simultaneous and automated ... Stable isotope labeling by amino acids in cell culture, SILAC, as a simple and accurate approach to expression proteomics Mol ... Here we describe a method, termed SILAC, for stable isotope labeling by amino acids in cell culture, for the in vivo ... isotopically labeled form of that amino acid, in this case deuterated leucine (Leu-d3). We find that growth of cells maintained ...
... bromo derivative was also prepared and should be a more reactive substrate for halogen interchange reactions or direct isotope ... Labeling pharmaceuticals with radioactive isotopes. Annual progress report, December 1, 1975--November 30, 1976 ... Blau, M. & Bender, M.A. Labeling pharmaceuticals with radioactive isotopes. Annual progress report, December 1, 1975--November ... bromo derivative was also prepared and should be a more reactive substrate for halogen interchange reactions or direct isotope ...
MS spectra of stable isotope-labeled GAPDH peptide AGAHLQGGAk. Panel B: Mass spectrometry spectra of stable isotope-labeled ... Also, because in vivo systems use stable isotope-labeled cell lines, all proteins in the cell become isotopically labeled, ... such as 15N-labeled E. coli or SILAC (i.e., stable isotope labeling using amino acids in cell culture), have been used to ... stable-isotope labeled human proteins with ,90% isotope incorporation in less than 8 hours. Heavy proteins expressed using this ...
For experiments at elevated abundances of the heavier isotope, the isotope fractionation factor αl (l for labeled compounds) ... 3) is valid to describe isotope fractionation of carbon and hydrogen isotopes only at low abundance of the heavier isotopes (13 ... D/H isotope fractionation factors α or ε and fitting parameter b obtained from studies with nonlabeled (αn) or labeled toluene ... Isotope fractionation factor α should be calculated from labeling experiments with equation 3 but are difficult to relate to ...
  • Quantitative analysis of SoPIL reagent-labeled peptides. (nih.gov)
  • Pairs of chemically identical peptides of different stable-isotope composition can be differentiated in a mass spectrometer owing to their mass difference. (wikipedia.org)
  • We have developed a multiplexed set of reagents for quantitative protein analysis that place isobaric mass labels at the N termini and lysine side chains of peptides in a digest mixture. (psu.edu)
  • The reagents are differentially isotopically labeled such that all derivatized peptides are isobaric and chromatographically indistinguishable, but yield signature or reporter ions following CID that can be used to identify and quantify individual members of the multiplex set. (psu.edu)
  • Stable isotope-labeled peptides are routinely used as internal standards for mass spectrometry (MS) quantification of enzymatically digested protein samples. (thermofisher.com)
  • As determined by MS analysis of heavy and light peptides, all expressed proteins had stable-isotope incorporation equal to or greater than 90% (Figure 3B and Figure 4). (thermofisher.com)
  • Panel B: Isotope incorporation from peptides derived from the proteins in Panel A. (thermofisher.com)
  • The resulting peptides were labeled with iTRAQ 114 and 117 reagents, respectively. (aacrjournals.org)
  • Both labeled peptides were then combined and analyzed simultaneously by nanoflow LC-QTOF MS for protein identification and quantitation. (aacrjournals.org)
  • We have used 15 N labeling of barley ( Hordeum vulgare ) plants and gas chromatography-mass spectrometry analysis of free amino acids and liquid chromatography-mass spectrometry analysis of proteins to track the enrichment of 15 N into the amino acid pools in barley leaves and then into tryptic peptides derived from newly synthesized proteins. (plantphysiol.org)
  • There was approximately a 9-h lag from label application until 15 N incorporation could be reliably quantified in extracted peptides. (plantphysiol.org)
  • AnaSpec has introduced ClearPoint™ Peptides, heavy isotope-labeled peptides for absolute quantitation (AQUA) of proteins. (technologynetworks.com)
  • AnaSpec's ClearPoint™ peptides offer a choice of heavy hydrogen (2H), carbon (13C), or nitrogen (15N)-isotopes specifically labeled at single, multiple or universal positions. (technologynetworks.com)
  • Targeted detection of peptides in a bottom-up strategy is the most common and precise mass spectrometry-based quantitation approach when combined with the use of stable isotope-labeled peptides. (proteincentre.com)
  • In this study, these methods were compared to a new approach using two different stable isotope-labeled standard (SIS) peptide isotopologues for each endogenous peptide to be quantified, enabling an external calibration curve as well as the quality control samples to be prepared in pooled human plasma without interference from endogenous peptides. (proteincentre.com)
  • Peptide sets and pools, as well as assay standardization kits are available with stable isotope labeled or unlabeled proteotypic peptides for mass-spectrometry based proteomics such as MRM assays. (jpt.com)
  • Interested in purchasing stable isotope labeled peptides? (jpt.com)
  • Stable isotopically labeled peptides (SIL) containing stable isotope labeled amino acids share the same physiochemical properties and (apart from a few exceptions) the same chemical reactivity with their non-labeled counterparts. (jpt.com)
  • However, under certain conditions labeled and unlabeled peptides behave differently. (jpt.com)
  • This is the basis for the use of stable isotope labeled peptides in a variety of applications, e.g. (jpt.com)
  • At JPT, we annually synthesize thousands of peptides with stable isotope labels. (jpt.com)
  • These properties make the stable isotope labeled peptides could be widely applied in quantification analysis (e.g. (creative-peptides.com)
  • As a world-leading peptide manufacturing organization, Creative Peptides possesses extensive expertise and years of experience which is able to provide customized stable isotope labeled peptides synthesizing service to customer worldwide. (creative-peptides.com)
  • All stable isotope labeled peptides are synthesized using the latest Fmoc solid-phase peptide technology and performed mass spectrometric analysis and stringent analytical HPLC to establish the final purity, to assure that our customers receive the highest quality peptides. (creative-peptides.com)
  • 2011). Absolute quantification of protein and post-translational modification abundance with stable isotope-labeled synthetic peptides. (creative-peptides.com)
  • Peptides labeled with stable, non-radioactive isotopes are increasingly used for convenient detection in research. (pepscan.com)
  • Stable isotope labeled (SIL) peptides display identical physiochemical properties and chemical reactivity as their non-labeled counterparts (apart from a few exceptions). (pepscan.com)
  • These SIL peptides are synthesized using only the highly enriched stable amino acids from premium supplier Cambridge Isotope Laboratories (CIL) for the Fmoc-based solid-phase custom peptide synthesis in our laboratory. (pepscan.com)
  • These important attributes have enabled us to successfully respond to the ever changing demand for best quality stable isotope peptides. (pepscan.com)
  • Stork BioChemicals provides custom stable isotope labeled ( 2 H, 13 C, 15 N) compounds (building blocks, drugs, drug candidates, active ingredients, metbolites, biomarkers, peptides…) to support your research and the development of your product. (storkbiochem.com)
  • We first demonstrate close to complete isotope incorporation for all peptides tested. (elsevier.com)
  • Dimethyl-SRM was further examined by comparing the relative quantitation of eight proteins in human CSF with the relative quantitation obtained using synthetic heavy peptides coupled to stable isotope dilution-SRM (SID-SRM). (elsevier.com)
  • Dimethyl labeling coupled with SRM provides a fast, convenient, and cost-effective alternative for relative quantitation of a large number of candidate proteins/peptides. (elsevier.com)
  • This study extended the applicability of stable-isotope dimethyl labeling to the economical and precise determination of food allergens and peptides. (preprints.org)
  • This labeling reaction is fast (less than 5 min) and complete without any detectable byproducts based on the analysis of MALDI and LC/ESI-MS/MS spectra of both derivatized and nonderivatized peptide standards and tryptic peptides of hemoglobin molecules. (ncku.edu.tw)
  • Terminal amine isotopic labeling of substrates (TAILS) is a method in quantitative proteomics that identifies the protein content of samples based on N-terminal fragments of each protein (N-terminal peptides) and detects differences in protein abundance among samples. (wikipedia.org)
  • TAILS is a 2D or 3D proteomics based assay for the labeling and isolation of N-terminal peptides, developed by a group at the University of British Columbia. (wikipedia.org)
  • This assay isolates the N-terminal peptides by removing the internal tryptic peptides via ultrafiltration leaving the labeled mature N-terminal and neo-N-Terminal peptides to be analyzed by tandem mass spectrometry (MS/MS). This negative selection allows the TAILS method to detect all N-termini in the given samples. (wikipedia.org)
  • The labeled N-termini of the original proteins remain blocked, while the new internal tryptic peptides have a free N-terminus. (wikipedia.org)
  • For proteins whose expression levels are the same in cells grown in the normal and labeled media, the relative areas of the normal (light) and labeled (heavy) isotopic peaks are linearly correlated with the cells mixing ratios. (nih.gov)
  • Methyl-specific isotopic labeling: a molecular tool box for solution NMR studies of large proteins. (sigmaaldrich.com)
  • Thus a combination of isotopic labeling and multidimensional, multinuclear experiments has significantly expanded the range of problems in structural biology amenable to NMR. (springer.com)
  • Stable Isotope Labeling by/with Amino acids in Cell culture (SILAC) is a technique based on mass spectrometry that detects differences in protein abundance among samples using non-radioactive isotopic labeling. (wikipedia.org)
  • The readily recognized isotopic doublet in the mass spectra of chlorine-containing compounds (due to the natural abundance of the 35 Cl and 37 Cl isotopes) has proven to be useful in the study of the metabolism of chlorine containing drugs such as chiorpromazine. (aspetjournals.org)
  • Since relatively few drugs contain elements having an appreciable natural abundance of more than one isotope, we have artificially created isotopic doublets to serve as conspicuous mass-spectral markers by mixing stable isotope labeled and unlabeled drugs. (aspetjournals.org)
  • Highlights DOI: 10.1002/anie.200602549 Photoaffinity Reagents Isotope-Labeled Photoaffinity Reagents and Mass Spectrometry To Identify Protein-Ligand Interactions** Andrea Sinz* Keywords: isotopic labeling · mass spectrometry · photoaffinity reagents · protein-ligand interactions The rate of drug discovery is greatly dependent on the development and improvement of rapid and reliable analytical methods for screening protein- ligand interactions. (docme.ru)
  • Here, we investigated the in vivo metabolic fluxes of individual reactions of the TCA cycle by developing two isotopic methods, 13 C tracing and fluxomics and the use of H/D isotope effects, with Xanthium strumarium leaves. (plantphysiol.org)
  • Leaves from a native New England tree species ( Fraxinus americana ) were collected at 1 to 3 h intervals over a 2-day experimental period, and, subsequently, the leaf wax δ 2 H isotopic ratios were measured using gas chromatography/isotope ratio mass spectrometry. (picarro.com)
  • 2008). Stable isotopic labeling of proteins for quantitative proteomic applications. (creative-peptides.com)
  • These comprise different isotopic patterns (from U- 13 C to single 13 C-labeled) and are supplied as neat standards in research and/or MPT (microbial and pyrogen tested) grade for MS- or NMR-based research applications. (isotope.com)
  • We supply the scientific community with stable isotope-labeled chemicals (many of which are produced by Cambridge Isotope Laboratories, Inc .), as well as isotopic gases, NMR solvents, isotopic metals, and clinical trials substrates. (eurisotop.com)
  • Isotopic labeling patterns are usually analyzed by mass spectrometry (MS) in the form of mass isotopomer distributions (MIDs), which are the mass-aggregated relative isotopologue abundances. (biomedcentral.com)
  • We offer deuterium (2H)-, 15N, 13C- labeled drug substance reference standards with high isotopic and chemical purity. (acanthusresearch.com)
  • Isotopic labeling (or isotopic labelling ) is a technique used to track the passage of an isotope (an atom with a detectable variation in neutron count) through a reaction , metabolic pathway , or cell . (wikipedia.org)
  • The position of the isotopes in the products is measured to determine the sequence the isotopic atom followed in the reaction or the cell's metabolic pathway. (wikipedia.org)
  • The nuclides used in isotopic labeling may be stable nuclides or radionuclides . (wikipedia.org)
  • An example of the use of isotopic labeling is the study of phenol (C 6 H 5 OH) in water by replacing common hydrogen ( protium ) with deuterium ( deuterium labeling ). (wikipedia.org)
  • An isotopic tracer , (also "isotopic marker" or "isotopic label"), is used in chemistry and biochemistry to help understand chemical reactions and interactions. (wikipedia.org)
  • NMR and MS detects isotopic differences, which allows information about the position of the labeled atoms in the products' structure to be determined. (wikipedia.org)
  • By studying the ratio between two isotopes of the same element, we avoid effects involving the overall abundance of the element, which usually swamp the much smaller variations in isotopic abundances. (wikipedia.org)
  • Further discussion of the application of isotopic tracers in geology is covered under the heading of isotope geochemistry . (wikipedia.org)
  • In theory, any element with two stable isotopes can be used as an isotopic tracer. (wikipedia.org)
  • This labeling strategy produces peaks differing by 28 mass units for each derivatized site relative to its nonderivatized counterpart and 4 mass units for each derivatized isotopic pair. (ncku.edu.tw)
  • BRIT has developed state-of-the-art methods and standardized procedures for the synthesis, purification and quality control /quality assurance of labelled compounds and biomolecules. (britatom.gov.in)
  • As Carbon and Hydrogen are fundamental components of all organic compounds, they can be replaced with their isotopes (Carbon-14 and Tritium) without altering their chemical properties. (britatom.gov.in)
  • Tritium labelled compounds are used as radiotracers in a wide range of studies such as in biochemistry, enzyme mechanisms, elucidation of organic reaction mechanisms and in environmental sciences for development of crop protection agents. (britatom.gov.in)
  • Carbon-14 labelled compounds are indispensable tools in the assessment of novel pharmaceuticals using absorption, distribution, metabolism & excretion (ADME) studies in living beings and in enzymatic and sub-cellular in vitro research applications. (britatom.gov.in)
  • The stable isotope labeled compounds market is projected to grow at a CAGR of 3.0% during the forecast period (2019-2024). (sandlerresearch.org)
  • However, the high cost of stable isotope-labeled compounds is expected to restrain the growth of this market during the forecast period. (sandlerresearch.org)
  • By type, the H-2 labeled compounds segment is expected to grow at the highest CAGR during the forecast period. (sandlerresearch.org)
  • Based on component, the stable isotope-labeled compounds market is segmented into C-13, N-15, O-18, H-2 (deuterium), and other isotopes. (sandlerresearch.org)
  • The H-2 labeled compounds segment accounted for the largest market share in 2018. (sandlerresearch.org)
  • This growth in this segment is driven primarily by growing drug research in the pharmaceutical industry and the increasing availability of deuterium-labeled compounds. (sandlerresearch.org)
  • By application, the research segment dominated the stable isotope labeled compounds market in 2018. (sandlerresearch.org)
  • Based on technology, the stable isotope-labeled compounds market is segmented into research, clinical diagnostics, industrial applications, and other applications. (sandlerresearch.org)
  • The research segment accounted for the largest share of the stable isotope-labeled compounds market in 2018, owing to rising research activities in the biomedical, pharmaceutical, environmental & ecological, and agricultural sectors. (sandlerresearch.org)
  • North America stable isotope labeled compounds market is expected to witness the highest growth during the forecast period. (sandlerresearch.org)
  • The report analyzes the stable isotope labeled compounds market and aims at estimating the market size and future growth potential. (sandlerresearch.org)
  • The research segment accounted for the largest share of the Stable Isotope Labeled Compounds Market in 2019. (gateo.de)
  • In 2019, North America dominated the Stable Isotope Labeled Compounds Market, followed by Europe. (gateo.de)
  • These isotope-labeled compounds are useful internal standards for quantification of TSNA and their metabolites in smokers in molecular epidemiological studies. (elsevier.com)
  • Journal of Labelled Compounds and Radiopharmaceuticals , 51 (5), 226-230. (elsevier.com)
  • Prof Lockley's current research interest is in the use of catalytic systems for labelling organic compounds with hydrogen isotopes. (surrey.ac.uk)
  • Stable isotope labeled compounds may be useful for bioanalytical purposes (to facilitate the quantification of analytes in biological matrix by LC/MS), for metabolomics & proteomics studies, for Magnetic Resonance Imaging (MRI), for medicinal chemistry purposes (by increasing half life of drug / drug candidate) as well as in structural biology. (storkbiochem.com)
  • Download our flyer dedicated to stable isotope labeled compounds here . (storkbiochem.com)
  • Since its acquisition by CIL in 2001, Eurisotop has become a leading producer of deuterated NMR solvents and is the leading supplier of stable isotope-labeled compounds in Europe. (eurisotop.com)
  • The radiation emitted by compounds containing the radioactive isotopes darkens a piece of photographic film , recording the position of the labeled compounds relative to one another in the gel. (wikipedia.org)
  • Eurisotop, a wholly owned subsidiary of Cambridge Isotope Laboratories, Inc. (CIL), was founded in January 1991 by a group of researchers from the Commissariat à l'Energie Atomique (CEA). (eurisotop.com)
  • Ninety-eight atom% 15 N-glycine (NLM-202-1, Cambridge Isotope Laboratories Inc, Andover, MA, USA) was used as a label. (biomedcentral.com)
  • Here, we developed a strategy by combining stable isotope labeling (IL) with liquid chromatography-mass spectrometry (LC-MS) under double precursor ion scan (DPI) and MRM for analysis of thiols in 5 types of human cancer urine. (nature.com)
  • Quantitative proteome analysis of oral tongue squamous cell carcinoma by laser capture microdissection, stable isotope labeling and liquid chromatography-mass spectrometry. (aacrjournals.org)
  • The protein expression profiles of dissected normal/cancer cells were examined using a quantitative proteomics approach based on stable isotope labeling and liquid chromatography-mass spectrometry (LC-MS). Both protein extracts were reduced, alkylated and digested with trypsin. (aacrjournals.org)
  • Our workflow, involving a stringent series of mass spectrometry filtering steps, demonstrates that 15 N labeling can be used for large-scale liquid chromatography-mass spectrometry studies of protein turnover in plants. (plantphysiol.org)
  • Relative quantitation of protein nitration by liquid chromatography-mass spectrometry using isotope-coded dimethyl labeling and chemoprecipitation. (semanticscholar.org)
  • Recently, stable isotope labeling (IL) strategy has been reported for non-targeted profiling of metabolites with the MS-based platform 19 . (nature.com)
  • The IL method greatly facilitates the spectral interpretation and metabolite identification because the light/heavy labeled metabolites are always detected as pairs in the mass spectra with characteristic mass difference 20 . (nature.com)
  • When trifluoroacetylated basic urinary extracts were examined by gas chromatography-mass spectrometry, the parent drug and any metabolites retaining the labeled site were readily identified by the presence of the M, M+3 doublet. (aspetjournals.org)
  • Mixtures containing dideuterium/nitrogen-15 labeled drug also were used in the studies of urinary metabolites and in studies of biliary metabolites. (aspetjournals.org)
  • Overall this dual labeling approach proved practical and valuable for the identification of polar metabolites by LC-MS/MS. (aspetjournals.org)
  • All Impurities,Metabolites & Labelled Isotopes are supplied with COA, Mass & H1NMR data, additional data would be provided at request with Nominal cost. (alfaomegapharma.com)
  • All Impurities,Metabolites & Labelled Isotopes listed are compound synthesized for pharmaceutical research and analytical testing only and are not intended for Diagnostic or Therapeutic use. (alfaomegapharma.com)
  • When I look at the distribution of labeling (MDV) I don't see any difference across the different metabolites but when I look at the relative abundance I see large differences. (stackexchange.com)
  • Following the feeding of [U- 13 C]glucose, the 13 C-label was incorporated into the various metabolites to different degrees depending on incubation time, metabolic pathway activity, and growth stage. (frontiersin.org)
  • Common stable isotopes used in research include 2H (Deuterium or D), 13C, 15N and 18O. (lgcstandards.com)
  • The deuterium-labelled and the unlabelled form of the drug to be investigated were simultaneously infused in 10 min. (biomedsearch.com)
  • The mean distribution and elimination half-lives were 8.4 +/- 5.9 min and 96 +/- 26 min for lidocaine, 9.2 +/- 7.0 min and 98 +/- 27 min for deuterium-labelled lidocaine, 15.3 +/- 9.9 min and 111 +/- 32 min for bupivacaine, and 15.2 +/- 10.9 min and 109 +/- 31 min for deuterium-labelled bupivacaine, respectively. (biomedsearch.com)
  • The mean volumes of the central compartment and mean steady state volumes of distribution were: lidocaine 37 +/- 151 and 97 +/- 201, deuterium-labelled lidocaine 39 +/- 161 and 98 +/- 181, bupivacaine 27 +/- 111 and 66 +/- 231 and deuterium-labelled bupivacaine 28 +/- 121, and 65 +/- 221, respectively. (biomedsearch.com)
  • 3 ) investigated the mechanism of isotope fractionation during toluene biodegradation using deuterium-labeled toluene. (asm.org)
  • However, in the experiments with labeled compound presented in the study, condition i is not fulfilled since the compound with deuterium accounts for 50% of the total toluene concentration. (asm.org)
  • By addition of deuterium-labeled methionine, we could observe the incorporation of new methyl marks on pre-existing tRNAs. (uni-muenchen.de)
  • We are characterising the effects of D2O and deuteration on the physico- chemical properties of bio-macromolecules in order to help optimise the crystallisation of deuterium- labelled biological macromolecules. (embl.fr)
  • For this he mostly employs the stable (non-radioactive) isotope deuterium. (surrey.ac.uk)
  • One-step exchange-labelling of pyridines and other N -heteroaromatics using deuterium gas: catalysis by heterogeneous rhodium and ruthenium catalysts, E Alexakis E, Jones JR, Lockley WJS, Tetrahedron Letters , 47, 5025-5028 (2006). (surrey.ac.uk)
  • One-step exchange-labelling of piperidines, piperazines and dialkylamines with deuterium oxide: catalysis by various ruthenium complexes. (surrey.ac.uk)
  • A solid-phase iridium-based ortho -exchange catalyst for the one-step labelling of aromatic substrates with deuterium, Hickey MJ, Kingston LP, Jones JR, Lockley WJS, Mather AN, Wilkinson DJ, Tetrahedron Letters , 45 8621-8623 (2004). (surrey.ac.uk)
  • Amino acid residue specific stable isotope labeling for quantitative proteomics. (nih.gov)
  • Labeling an entire proteome with heavy amino acids in vivo generates an ideal standard for quantitative proteomics. (isotope.com)
  • Quantitative proteomics has traditionally been performed by two-dimensional gel electrophoresis, but recently, mass spectrometric methods based on stable isotope quantitation have shown great promise for the simultaneous and automated identification and quantitation of complex protein mixtures. (nih.gov)
  • In this paper, we report a novel, stable-isotope labeling strategy for quantitative proteomics that uses a simple reagent, formaldehyde, to globally label the N-terminus and ε-amino group of Lys through reductive amination. (ncku.edu.tw)
  • Although E. coli is the organism of choice for expression of proteins in isotope labeled form for NMR studies, bacterial cells have limitations concerning their ability of producing soluble and well-folded proteins of human origin. (nih.gov)
  • In order to study these proteins by NMR, they are ideally prepared in isotope labeled form. (nih.gov)
  • Various stable isotope labeling (SIL) techniques have recently emerged to improve the efficiency and accuracy of protein quantitation by mass spectrometry (MS). We have developed a mass-tagging strategy to incorporate stable isotope tagged amino acids into cellular proteins in a residue-specific manner during cell growth. (nih.gov)
  • We present the design and synthesis of a new quantitative strategy termed soluble polymer-based isotope labeling (SoPIL) and its application as a novel and inclusive method for the identification and relative quantification of individual proteins in complex snake venoms. (nih.gov)
  • The exchange of naturally occurring amino acids with their nonradioactive isotopically labeled versions in a growth medium leads to the incorporation of these "heavy" amino acids into the newly synthesized proteins. (asm.org)
  • However, stable isotope-labeled proteins are considered better standards because they control for MS sample-preparation loss and digestion efficiency variation. (thermofisher.com)
  • Also, because in vivo systems use stable isotope-labeled cell lines, all proteins in the cell become isotopically labeled, leading to high waste and cost. (thermofisher.com)
  • 2 however, these systems lack the components needed for proper folding and modification of human proteins, have low expression yields, and inefficiently incorporate stable isotope-labeled amino acids. (thermofisher.com)
  • The 1-Step Heavy Protein IVT Kit uses a novel human cell-free lysate 3,4 to rapidly express stable isotope-labeled proteins (Figure 1). (thermofisher.com)
  • Cell lysates are combined with the reaction mixture, vector DNA and stable isotope-labeled amino acids to express recombinant proteins. (thermofisher.com)
  • Human proteins expressed by in vitro translation have high isotope incorporation. (thermofisher.com)
  • Here we describe a method, termed SILAC, for stable isotope labeling by amino acids in cell culture, for the in vivo incorporation of specific amino acids into all mammalian proteins. (nih.gov)
  • The phosphorylated proteins are then isolated and identified by mass spectrometry, in which the heavy phosphate (+85.979 Da) labeled phosphopeptides reveal the kinase specificity. (mcponline.org)
  • This technology, termed stable isotope labeling with amino acids in cell culture (SILAC), has recently gained popularity for its ability to compare the expression levels of hundreds of proteins in a single experiment. (mcponline.org)
  • SILAC makes use of 12 C- and 13 C-labeled amino acids added to the growth media of separately cultured cell lines, giving rise to cells containing either "light" or "heavy" proteins, respectively. (mcponline.org)
  • Stable isotope labeling with amino acids in cell culture (SILAC) is a simple and straightforward approach for in vivo incorporation of a tag into proteins for relative quantitation by mass spectrometry. (sciencemag.org)
  • Using information on the rate of growth of barley leaves combined with the rate of degradation of 14 N-labeled proteins, we calculate the turnover rates of 508 different proteins in barley and show that they vary by more than 100-fold. (plantphysiol.org)
  • Photoaffinity labeling presents a valuable method for studying the interactions of biologically active, small molecules with their target proteins. (docme.ru)
  • Moreover, the reactions of the photoaffinity label with proteins should lead to stable and homogeneous products that can be isolated, purified, and analyzed subsequently by mass spectrometry. (docme.ru)
  • Reproducible high-efficiency labeling of target proteins is achieved with phenyl azides, diazirines, and benzophenone photophores. (docme.ru)
  • We offer non radioactive stable isotope labeling of proteins and antibodies. (antibodyresearch.com)
  • Bacterial transport proteins that scavenge hydantoins have been identified, isolated and characterised with isotope-labelling of hydantoins as an essential requirement to achieve this. (openmedscience.com)
  • Isotope-labelled hydantoins have been used in a range of biological, biomedical, food and environmental applications including metabolic and in vivo tissue distribution studies, biochemical analysis of transport proteins, identification and tissue distribution of drug binding sites, drug metabolism and pharmacokinetic studies and as an imaging agent. (openmedscience.com)
  • This reaction is catalyzed by sodium cyanoborohydride and attaches the labeled methyl groups to lysine-amines and the free (∝)- amino groups at the N-termini of the proteins and protease cleavage products. (wikipedia.org)
  • Its characteristic peptide bond fragment ions, type b and y ions, are labeled. (nih.gov)
  • The advantages of this metabolic labeling technique are: increased confidence in phosphorylated peptide identification, direct labeling of phosphorylation sites, measurement phosphorylation rates, and the identification of actively phosphorylated sites in a cell-like environment. (umd.edu)
  • When a heavy labeled proteome is mixed with an unlabeled proteome then digested, every unlabeled peptide identified by the mass spectrometer can be quantified by its corresponding heavy peptide. (isotope.com)
  • In SILAC, the tryptic amino acids, arginine (R) and lysine (K), contain heavy stable isotopes, so if digesting with trypsin, every peptide is labeled. (isotope.com)
  • We have developed a method by which 15 N-labeled nitrotyrosine groups are generated on peptide or protein standards using stable isotope-labeled peroxynitrite (O 15 NOO − ), and the resulting standard is mixed with representative samples in which nitrotyrosine formation is to be measured by mass spectrometry (MS). Nitropeptide MS/MS spectra are filtered using high mass accuracy Fourier transform MS (FTMS) detection of the nitrotyrosine immonium ion. (mdpi.com)
  • Panel A: MS spectra of stable isotope-labeled GAPDH peptide AGAHLQGGAk. (thermofisher.com)
  • Panel B: Mass spectrometry spectra of stable isotope-labeled cyclin D1 peptide AYPDANLLNDr. (thermofisher.com)
  • By spiking protein tryptic digests with internal heavy isotope labeled peptide standards, MS becomes an absolute quantitation (AQUA) method. (technologynetworks.com)
  • Multiplexed MRM-Based Protein Quantitation Using Two Different Stable Isotope-Labeled Peptide Isotopologues for Calibration. (proteincentre.com)
  • TAILS requires only small sample of peptide for experimentation (100-300 ug), can be used with proteases which have unknown or broad specificity and supports a variety of methods for sample labeling. (wikipedia.org)
  • Stable-isotope dimethyl labeling is a highly reactive and cost-effective derivatization procedure that could be utilized in proteomics analysis. (preprints.org)
  • The IL strategy has also been applied in metabolomics for the relative quantification and/or absolute quantification by calculating the peak intensity ratios of the isotope labeled peak pairs in two comparative samples (or in one sample and standards) 23 . (nature.com)
  • At this stage in development, stable isotope labeled [ 13 C 6 ]KAF156-1 was available as the internal standard for the quantification of KAF156. (aspetjournals.org)
  • Relative quantification of biomarkers using mixed-isotope labeling coupled with MS. (semanticscholar.org)
  • The chapter is concluded with case studies, describing successful application of isotope labeling in insect cells for NMR studies including solid-state experiments, ligand binding studies and protein dynamics. (nih.gov)
  • This strategy is generally applicable using any particular type of amino acid as the labeling precursors for accurate quantitation of protein relative abundances. (nih.gov)
  • Growing microorganisms incorporate isotopically labeled amino acids, increasing protein masses and thereby leading to mass shifts of their corresponding peaks in the profile spectra. (asm.org)
  • The ICAT quantitative labeling strategy (3, 4) is perhaps the best-characterized method for relative protein quantitation using MS. Other elegant approaches use cell-culture enrichment with a stable isotope-labeled amino acid, including arginine (5), lysine (6), tyrosine (7), and leucine (8), for in vivo incorporation of a mass difference to support relative quantitation. (psu.edu)
  • We have recently developed the stereo-array isotope labeling (SAIL) method to overcome the size problem of NMR structure determination by utilizing a protein exclusively composed of stereo- and regio-specifically isotope-labeled amino acids. (rcsb.org)
  • The SAIL protein yielded less crowded and better resolved spectra than uniform (13)C and (15)N labeling, and enabled the homodimeric solution structure of this protein to be determined. (rcsb.org)
  • In this study, we used the Thermo Scientific 1-Step Heavy Protein IVT Kit (Part No. 88331 ) to express stable isotope-labeled protein standards for quantitative MS. (thermofisher.com)
  • In order to screen for cellular substrates of the Bacillus subtilis BY-kinase PtkA, and its cognate phosphotyrosine-protein phosphatase PtpZ, we performed a triple Stable Isotope Labeling by Amino acids in Cell culture-based quantitative phosphoproteome analysis. (nih.gov)
  • Isotope-Labeled Photoaffinity Reagents and Mass Spectrometry To Identify ProteinЦLigand Interactions. (docme.ru)
  • 1] In photoaffinity labeling, a covalent linkage is created between a ligand and a protein upon irradiation by UV light. (docme.ru)
  • The requirements for the ideal photoaffinity label include its chemical stability prior to photoactivation, its easy photolysis at wavelengths that do not cause photochemical damage to the protein, and high reactivity of the intermediate product to C H groups and to nucleophilic X atoms from X H groups. (docme.ru)
  • which contains the following subunits:[6] * A) a reactive site for coupling the biologically active ligand, * B) a photoreactive site for reaction with the target protein, * C) a biotin label, which allows purification of the protein-ligand com- [*] Priv. (docme.ru)
  • Int. Ed. 2007, 46, 660 - 662 Angewandte Chemie * plex by affinity chromatography on avidin beads, D) a stable isotope label to facilitate mass spectrometric identification of the protein-ligand complex. (docme.ru)
  • high concentrations of isotope were discovered in the non-protein fraction of the serum of foetuses in the control uteri. (royalsocietypublishing.org)
  • With the stable isotope-labeled amino acid, the deuteration of protein can be attained without damaging the NMR sensitivity of remaining hydrogen nucleus and, in addition, the rapid, accurate analysis of NMR spectrum of a high-molecular protein which is beyond the limitation in the prior art and the determination of the stereo-structure can be performed at the same time. (go.jp)
  • 5. A method for producing a target protein composed of stable isotope-labeled amino acids, which comprises synthesizing a cell-free protein by using the combination of the stable isotope-labeled amino acids set forth in claim 1. (go.jp)
  • 6. The method for producing a target protein according to claim 5 wherein a combination of stable isotope-labeled amino acids of claim 2 is used as the all amino acids constituting the target protein. (go.jp)
  • 9. A method for analyzing the structure of a target protein using NMR, which comprises analyzing the structure of the target protein, in which all the amino acids constituting the target protein are replaced with the stable isotope-labeled amino acids of claim 1, by NMR spectral determination. (go.jp)
  • 10. The method for analyzing the structure of a target protein using NMR according to claim 9 wherein all the amino acids constituting the target protein are replaced with the stable isotope-labeled amino acids of claim 2. (go.jp)
  • 131I-labelled Human Serum Albumin and 125I-sodium iodide were used to measure protein loss from the peritoneum and peritoneal clearance of iodide in a group of 8 uraemic patients, each one being studied after a different number of dialyses. (semanticscholar.org)
  • For each concentration, equal volumes of unlabeled and uniformly 15 N-labeled protein (≈1.5 pmol/μl) protein were mixed, and 10-μl aliquots of the resulting samples were subjected to SDS/PAGE (4-20% gradient Tris-glycine gel, NOVEX, San Diego. (pnas.org)
  • In this manuscript, we present a proof-of-concept study for targeted relative protein quantitation workflow using chemical labeling in the form of dimethylation, coupled with selected reaction monitoring (dimethyl-SRM). (elsevier.com)
  • The advantage of metabolic labeling over in vitro tagging techniques is that the heavy and unlabeled samples are mixed before sample preparation, preventing variability between preparations from distorting the final quantitation results. (isotope.com)
  • Accurate quantitation is achieved through the use of whole-cell stable isotope labeling. (pnas.org)
  • Axetris MFCs used for CO2 isotope tracking Swiss Federal Laboratories for Materials Science and Technology (Empa) is using Axetris mass flow controllers (MFCs) in its research laboratory at the Jungfraujoch High Altitude Research Station, located at the "Top of Europe. (environmental-expert.com)
  • Here, we report metabolic isotope labeling to produce isotopically labeled internal standards in bacteria and yeast. (mdpi.com)
  • This method demonstrates the generalizability of SILEC, and provides a simple method for generating a library of stable isotope labeled internal standards for quantifying and tracing the metabolism of cellular and tissue NAD(H) and NADP(H). (rsc.org)
  • Using biosynthetic stable isotope labeled internal standards we quantified 12 modified nucleosides in tRNA from S. cerevisiae over 24 hours. (uni-muenchen.de)
  • A SILAC approach involving incorporation of tyrosine labeled with nine carbon-13 atoms (13C) instead of the normal carbon-12 (12C) has been utilized to study tyrosine kinase substrates in signaling pathways. (wikipedia.org)
  • Pulsed SILAC (pSILAC) is a variation of the SILAC method where the labelled amino acids are added to the growth medium for only a short period of time. (wikipedia.org)
  • It had also been used to study biofilm tolerance to antibiotics to differentiate tolerant and sensitive subpopulations Traditionally the level of multiplexing in SILAC was limited due to the number of SILAC isotopes available. (wikipedia.org)
  • Recently, a new technique called NeuCode (neutron encoding) SILAC, has augmented the level of multiplexing achievable with metabolic labeling (up to 4). (wikipedia.org)
  • The NeuCode amino acid method is similar to SILAC but differs in that the labeling only utilizes heavy amino acids. (wikipedia.org)
  • SILAC refers to labeling cultured cells with heavy amino acids for quantitative proteomic analysis. (isotope.com)
  • Please visit the NeuCode™ section of Thermo's SILAC Metabolic Labeling Systems webpage for more information on the NeuCode™ amino acids. (isotope.com)
  • Stable Isotope Labeling by Amino acids in Cell culture (SILAC) screen: DnaK phosphorylation at Y601 depends on PtkA and PtpZ. (nih.gov)
  • Although seen continued ipse not, only sensible constant coal-fields Had known to observe long in being the download stable isotope labeling by amino acids in cell culture silac of job draughts. (sftv.org)
  • unavoidably for ourselves, not,( numbers as we suppose), we must design of download stable isotope labeling by amino acids in cell culture silac methods and not as we should of the right hatred and of some flagrant heat of the intention,( antecedent) that we at all companions sit to fortune, if it depends almost be to us. (sftv.org)
  • Differential stable isotope labeling analysis provides qualitative metabolic flux information in a non-targeted manner. (biomedcentral.com)
  • This metabolic labeling strategy has been employed by hundreds of proteomic studies (see example references below). (isotope.com)
  • For additional information, refer to our Stable Isotope-Labeled Products for Metabolic Research catalog. (isotope.com)
  • We present a novel workflow to analyze non-targeted metabolome-wide stable isotope labeling data to detect metabolic flux changes in a non-targeted manner. (biomedcentral.com)
  • As stated above, MIDs from stable isotope labeling experiments hold metabolic flux information. (biomedcentral.com)
  • Measuring the propagation of the label through metabolic networks may provide information on carbon allocation in sink fruit during fruit development. (frontiersin.org)
  • In this research, gas chromatography coupled to mass spectrometry based metabolite profiling was used to characterize the changing metabolic pool sizes in developing apple fruit at five growth stages (30, 58, 93, 121, and 149 days after full bloom) using 13 C-isotope feeding experiments on hypanthium tissue discs. (frontiersin.org)
  • Isotope-labeled photoaffinity reagents 1[6] and 2. (docme.ru)
  • The incorporation of stable isotopes also allows for a quantitative comparison between the two samples. (mcponline.org)
  • We describe an efficient in vivo method for measuring hippocampal neurogenesis involving incorporation of the stable isotope, 2 H, into genomic DNA during labeling with 2 H 2 O (heavy water). (aspetjournals.org)
  • Fluoxetine treatment increased 2 H incorporation into DNA of gradient-enriched neurons or flow-sorted neuronal nuclei 4 weeks after 2 H 2 O labeling, representing the survival and differentiation of newly divided cells into neurons. (aspetjournals.org)
  • One of the major advancements in NMR spectroscopy of biomolecules has been the development of methods for isotope labeling. (springer.com)
  • Isotope Labeling of Biomolecules: Applications, the latest in the Methods in Enzymology series, focuses on stable isotope labeling methods and applications for biomolecules. (indigo.ca)
  • Advances in the application of stable isotopes have allowed the quantitative evaluation of previously cryptic ecological processes. (mammalogy.org)
  • For example, the medium can contain arginine labeled with six carbon-13 atoms (13C) instead of the normal carbon-12 (12C). (wikipedia.org)
  • 2. The combination of stable isotope-labeled amino acids of claim 1, wherein when said pattern is (d), then when the aromatic ring has hydrogen atoms, the hydrogen atoms in the aromatic ring are partially deuterated. (go.jp)
  • 4. The combination of stable isotope-labeled amino acids of claim 3, wherein carbon atoms constituting carbonyl group and guanidyl group in the amino acids are replaced with 13C. (go.jp)
  • Isotope labeled or heavy amino acids are derived from natural amino acids by substitution of atoms with respective heavy isotopes. (jpt.com)
  • Stable isotope labeled, or 'heavy' amino acids, are derived from natural amino acids by substitution of certain atoms (N, C, H) with their 'heavy isotope' variant. (pepscan.com)
  • The number, nature and location of stable isotope label atoms (either 2 H, 13 C or 15 N or a combination of them) on your molecular framework can be tuned to your specific needs. (storkbiochem.com)
  • The reactant is 'labeled' by replacing specific atoms by their isotope. (wikipedia.org)
  • In this technique, one or more of the atoms of the molecule of interest is substituted for an atom of the same chemical element , but of a different isotope (like a radioactive isotope used in radioactive tracing ). (wikipedia.org)
  • We report here a novel proteomic strategy to identify the specificity and direct substrates of kinases by coupling phosphoproteomics with a sensitive stable isotope labeled kinase reaction. (mcponline.org)
  • In recent years, the application of isotopically labeled substrates has received extensive attention in plant physiology. (frontiersin.org)
  • At the same time there has been a rapid progress in the number of NMR experiments that utilize such isotope labeled samples. (springer.com)
  • For several of their experiments they obtained negative hydrogen isotope fractionation factors (see Table 1 in reference 3 ), which contradict the definition of the fractionation factor (see below). (asm.org)
  • However, our recent isotope labeling experiments on a grass species ( Phleum pratense ) yielded different conclusions from published data on a tree species ( Populus trichocarpa ), with the former showing rapid regeneration and the latter little regeneration in mature leaves. (picarro.com)
  • To address this challenge, aqueous reduction experiments with an Al2O3-supported Pd/In bimetallic catalyst were conducted using isotope-labeled nitrite (15NO2-), the first reduction intermediate of NO3-, alone and in combination with unlabeled proposed reduction intermediates (N2O, NO), and using N2O and NO alone, each as a starting reactant. (illinois.edu)
  • The use of an additional isotope would allow dual-labelling of two groups of males to determine for instance paternity in competition experiments, or to study multiple mating events. (biomedcentral.com)
  • Survival of larvae from labelled or control ( i.e. unlabelled) treatments was determined for three experiments, and in each experiment two trays per treatment were used. (biomedcentral.com)
  • To determine adult male longevity, newly emerged males of the 15 N-labelled tray and the control tray were placed in a standard rearing cage to monitor survival ( N = 25) and this was replicated twice using males from different experiments (in one of the experiments 3 duplicates were performed). (biomedcentral.com)
  • Using 15N stable isotope as a tracer to quantify N transformation rates in isotope-enrichment experiments improves understanding of the N cycle in various ecosystems. (environmental-expert.com)
  • The usage of matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) profiling in combination with growth media containing isotopically labeled amino acids facilitates the detection of resistant microorganisms after 3 h or less directly from the profile spectrum. (asm.org)
  • The table below lists the most common stable isotopically labeled amino acids. (jpt.com)
  • The authors overlooked that the Rayleigh equation that is normally used to evaluate isotope fractionation at natural abundance level ( 2 ) is not applicable to studies with labeled substrate, particularly if large isotope fractionation occurs. (asm.org)
  • The crucial point is that L / L 0 in equation 5 can only be approximated by f if either (i) the concentrations of the heavy isotopes, H and H 0 , are small, as common for studies at natural abundance level, or (ii) 1 + R ≈ 1 + R 0 . (asm.org)
  • In particular, researchers have utilized the predictable spatial patterning in natural abundance of isotopes to better understand animal dispersal and migration. (mammalogy.org)
  • Separating soil respiration components with stable isotopes: natural abundance and labelling approaches. (sisef.it)
  • Isotope tracers are commonly used in the form of isotope ratios. (wikipedia.org)
  • mathtex$$\[{\alpha}{=}\frac{H/L}{\mathrm{dH}_{\mathrm{p}}/\mathrm{dL}_{\mathrm{p}}}\]$$mathtex$$ (1) where H and L are the concentrations of the substrate with heavy and light isotopes, respectively, at a given time and dH p and dL p are increments of product with heavy or light isotopes, respectively, that appear in an infinitely short time (instantaneous product). (asm.org)
  • However, the most commonly used stable isotope tracers involve relatively light isotopes, which readily undergo fractionation in natural systems. (wikipedia.org)
  • Because the labeled atom has the same number of protons, it will behave in almost exactly the same way as its unlabeled counterpart and, with few exceptions, will not interfere with the reaction under investigation. (wikipedia.org)
  • In this example the labelling reaction (reductive dimethylation) also blocks the reactive amino groups. (wikipedia.org)
  • This hydrogen isotope can be handled without special precautions and has important uses in mass spectrometry. (surrey.ac.uk)
  • 30 Years with ortho -directed hydrogen isotope exchange labelling, Lockley WJS, J Label Compd Radiopharm , 50, 779-788 (2007). (surrey.ac.uk)
  • 2014 . Quantitative, time-resolved proteomic analysis by combining bioorthogonal noncanonical amino acid tagging and pulsed stable isotope labeling by amino acids in cell culture. (isotope.com)
  • Both stable isotopes can be analysed simultaneously in a mass spectrometer, offering the possibility to detect both labels in one sample in order to study complex and difficult-to-detect mating events, such as multiple mating. (biomedcentral.com)
  • 2018. Multiplexed proteome analysis with neutron-encoded stable isotope labeling in cells and mice. (isotope.com)
  • This book is intended to serve as a compendium of isotope labeling for the biomolecular NMR community providing comprehensive coverage of the existing methods and latest developments along with protocols and practical hints on the various experimental aspects. (springer.com)
  • This practical guide to biomolecular labeling looks at new techniques that are becoming widely used. (indigo.ca)
  • Zvi Kelman is the Director of the Biomolecular Labeling Laboratory (BL2), National Institute of Standards and Technology (NIST). (indigo.ca)
  • In contrast, the second population is fed with growth medium containing amino acids labeled with stable (non-radioactive) heavy isotopes. (wikipedia.org)
  • Mammalian cell lines are grown in media lacking a standard essential amino acid but supplemented with a non-radioactive, isotopically labeled form of that amino acid, in this case deuterated leucine (Leu-d3). (nih.gov)
  • Labeling pharmaceuticals with radioactive isotopes. (unt.edu)
  • Radioactive isotopes can be tested using the autoradiographs of gels in gel electrophoresis . (wikipedia.org)
  • A radiogenic isotope tracer [2] involves an isotope produced by radioactive decay , which is usually in a ratio with a non-radiogenic isotope (whose abundance in the earth does not vary due to radioactive decay). (wikipedia.org)
  • By combination of 2 differentially isotope labeled media we developed NAIL-MS, nucleic acid isotope labeling coupled mass spectrometry. (uni-muenchen.de)
  • How can I interpret this, when the labeling patterns are the same but the relative abundance is significantly different? (stackexchange.com)
  • Other labeled amino acids are available upon request. (jpt.com)
  • . 4 These 15 N-Microcystin standards are ideally suited for LC-MS/MS analysis using isotope dilution mass spectrometry, having an identical chemical structure, and with seven or more mass units separation from the native analyte. (isotope.com)
  • Analysis of isotope-containing hydantoins by NMR spectroscopy has been important for confirming purity, labelling integrity, specific activity and molecule conformation. (openmedscience.com)
  • In this chapter different strategies for isotope labeling in insect cells are described - uniform and amino acid specific. (nih.gov)
  • A general introduction to expression with baculovirus infected insect cells is given followed by a detailed descriptions of labeling approaches. (nih.gov)
  • Tritium labelling via an iridium-based solid-phase catalyst. (surrey.ac.uk)
  • We utilized the essential requirement of niacin and tryptophan for NAD synthesis in mammalian cells to devise a stable isotope labeling by essential nutrients in cell culture (SILEC) method for efficient labeling of intracellular NAD(H) and NADP(H) pools. (rsc.org)
  • Using a two-label system incorporating [ 13 C 3 15 N 1 ]-nicotinamide and [ 13 C 11 ]-tryptophan, we quantify the relative contribution of salvage and de novo NAD synthesis, respectively, in S. cerevisiae and HepG2 human hepatocellular carcinoma cells under basal conditions. (rsc.org)
  • To improve analysis of this compound, CIL collaborated with researchers at North Carolina State University to offer a new 13 C 3 / 15 N 2 labeled BMAA standard. (isotope.com)
  • Since labeled compound will likely be used in further investigations to study isotope fractionation, it is important to demonstrate why the commonly used Rayleigh equation is usually not applicable in such studies and to provide an alternative method to quantify isotope fractionation. (asm.org)
  • Based on application, the Stable Isotope Labeled Compound Market is segmented into research, clinical diagnostics, industrial applications, and other applications. (gateo.de)
  • Please inquire if an alternate compound, label, or quantity is required. (isotope.com)
  • Still, especially the combination of Free Air Carbon Enrichment (FACE) with dual δ 13 C and δ 18 O stable isotope approach has the potential to provide new answers on the response sensitivity of turnover dynamics of the largest belowground soil carbon storage to elevated temperature and CO 2 . (sisef.it)
  • or b) a labelled 13 CO 2 signal is applied to compartments of the soil carbon cycle. (sisef.it)
  • The heavier stable isotope 13 C is much less abundant than the lighter 12 C. 13 C ≈ 1.10% of the total carbon ( [5] ). (sisef.it)
  • Were those carbon sources labeled? (stackexchange.com)
  • In previous studies it was determined that the stable isotope 13-carbon can be used as a semen label to detect mating events in the malaria mosquito Anopheles arabiensis . (biomedcentral.com)
  • A carbon-13 label was used to determine the mechanism in the 1,2- to 1,3-didehydrobenzene conversion of the phenyl substituted aryne precursor 1 to acenaphthylene. (wikipedia.org)
  • Unfortunately, [ 14 C]KAF156-1 was uniformly labeled ( n = 1-6) with the mass ratio of ∼0.1. (aspetjournals.org)
  • In this paper we describe the use of an additional stable isotope, 15-nitrogen ( 15 N), for that same purpose. (biomedcentral.com)
  • In this paper, we investigated the use of the stable isotope 15-nitrogen ( 15 N) as a semen label for mosquito mating. (biomedcentral.com)