A non-metabolizable galactose analog that induces expression of the LAC OPERON.
Galactosides in which the oxygen atom linking the sugar and aglycone is replaced by a sulfur atom.
A disaccharide of GLUCOSE and GALACTOSE in human and cow milk. It is used in pharmacy for tablets, in medicine as a nutrient, and in industry.
A family of galactoside hydrolases that hydrolyze compounds with an O-galactosyl linkage. EC 3.2.1.-.
An isomer of 1-PROPANOL. It is a colorless liquid having disinfectant properties. It is used in the manufacture of acetone and its derivatives and as a solvent. Topically, it is used as an antiseptic.
Enzymes catalyzing the transfer of an acetyl group, usually from acetyl coenzyme A, to another compound. EC 2.3.1.
Salts and esters of the 14-carbon saturated monocarboxylic acid--myristic acid.
A species of gram-negative, facultatively anaerobic, rod-shaped bacteria (GRAM-NEGATIVE FACULTATIVELY ANAEROBIC RODS) commonly found in the lower part of the intestine of warm-blooded animals. It is usually nonpathogenic, but some strains are known to produce DIARRHEA and pyogenic infections. Pathogenic strains (virotypes) are classified by their specific pathogenic mechanisms such as toxins (ENTEROTOXIGENIC ESCHERICHIA COLI), etc.
A colorless liquid made by oxidation of aliphatic hydrocarbons that is used as a solvent and chemical intermediate.
Proteins found in any species of bacterium.
A serotype of Salmonella enterica that is a frequent agent of Salmonella gastroenteritis in humans. It also causes PARATYPHOID FEVER.
In GRAM NEGATIVE BACTERIA, multiprotein complexes that function to translocate pathogen protein effector molecules across the bacterial cell envelope, often directly into the host. These effectors are involved in producing surface structures for adhesion, bacterial motility, manipulation of host functions, modulation of host defense responses, and other functions involved in facilitating survival of the pathogen. Several of the systems have homologous components functioning similarly in GRAM POSITIVE BACTERIA.
A genus of gram-negative, facultatively anaerobic, rod-shaped bacteria that utilizes citrate as a sole carbon source. It is pathogenic for humans, causing enteric fevers, gastroenteritis, and bacteremia. Food poisoning is the most common clinical manifestation. Organisms within this genus are separated on the basis of antigenic characteristics, sugar fermentation patterns, and bacteriophage susceptibility.
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
Infections with bacteria of the genus SALMONELLA.
A genus of gram-negative, aerobic, rod-shaped bacteria that activate PLANT ROOT NODULATION in leguminous plants. Members of this genus are nitrogen-fixing and common soil inhabitants.
Techniques for labeling a substance with a stable or radioactive isotope. It is not used for articles involving labeled substances unless the methods of labeling are substantively discussed. Tracers that may be labeled include chemical substances, cells, or microorganisms.
Chromatographic techniques in which the mobile phase is a liquid.
The protein complement of an organism coded for by its genome.
The systematic study of the complete complement of proteins (PROTEOME) of organisms.
Comprehensive, methodical analysis of complex biological systems by monitoring responses to perturbations of biological processes. Large scale, computerized collection and analysis of the data are used to develop and test models of biological systems.
Atomic species differing in mass number but having the same atomic number. (Grant & Hackh's Chemical Dictionary, 5th ed)
Members of the class of compounds composed of AMINO ACIDS joined together by peptide bonds between adjacent amino acids into linear, branched or cyclical structures. OLIGOPEPTIDES are composed of approximately 2-12 amino acids. Polypeptides are composed of approximately 13 or more amino acids. PROTEINS are linear polypeptides that are normally synthesized on RIBOSOMES.
A portion of the animal phylum Chordata comprised of the subphyla CEPHALOCHORDATA; UROCHORDATA, and HYPEROTRETI, but not including the Vertebrata (VERTEBRATES). It includes nonvertebrate animals having a NOTOCHORD during some developmental stage.
Phylum in the domain Eukarya, comprised of animals either with fully developed backbones (VERTEBRATES), or those with notochords only during some developmental stage (CHORDATA, NONVERTEBRATE).
Triple-looped protein domains linked by disulfide bonds. These common structural domains, so-named for their resemblance to Danish pastries known as kringlers, play a role in binding membranes, proteins, and phospholipids as well as in regulating proteolysis. Kringles are also present in coagulation-related and fibrinolytic proteins and other plasma proteinases.
The only species of a cosmopolitan ascidian.
Small fish-like marine creatures often used in phylogenetic comparative studies of CHORDATES.
The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.
Granulated cells that are found in almost all tissues, most abundantly in the skin and the gastrointestinal tract. Like the BASOPHILS, mast cells contain large amounts of HISTAMINE and HEPARIN. Unlike basophils, mast cells normally remain in the tissues and do not circulate in the blood. Mast cells, derived from the bone marrow stem cells, are regulated by the STEM CELL FACTOR.
Exclusive legal rights or privileges applied to inventions, plants, etc.
Toxins produced, especially by bacterial or fungal cells, and released into the culture medium or environment.
A species of gram-negative, aerobic, rod-shaped bacteria commonly isolated from clinical specimens (wound, burn, and urinary tract infections). It is also found widely distributed in soil and water. P. aeruginosa is a major agent of nosocomial infection.
Enzymes that transfer the ADP-RIBOSE group of NAD or NADP to proteins or other small molecules. Transfer of ADP-ribose to water (i.e., hydrolysis) is catalyzed by the NADASES. The mono(ADP-ribose)transferases transfer a single ADP-ribose. POLY(ADP-RIBOSE) POLYMERASES transfer multiple units of ADP-ribose to protein targets, building POLY ADENOSINE DIPHOSPHATE RIBOSE in linear or branched chains.
An immunoglobulin associated with MAST CELLS. Overexpression has been associated with allergic hypersensitivity (HYPERSENSITIVITY, IMMEDIATE).
Molecules found on the surface of some, but not all, B-lymphocytes, T-lymphocytes, and macrophages, which recognize and combine with the Fc (crystallizable) portion of immunoglobulin molecules.
An enzyme of the hydrolase class that catalyzes the reaction of triacylglycerol and water to yield diacylglycerol and a fatty acid anion. It is produced by glands on the tongue and by the pancreas and initiates the digestion of dietary fats. (From Dorland, 27th ed) EC 3.1.1.3.
A characteristic feature of enzyme activity in relation to the kind of substrate on which the enzyme or catalytic molecule reacts.
An enzyme of the hydrolase class that catalyzes the reaction of triacylglycerol and water to yield diacylglycerol and a fatty acid anion. The enzyme hydrolyzes triacylglycerols in chylomicrons, very-low-density lipoproteins, low-density lipoproteins, and diacylglycerols. It occurs on capillary endothelial surfaces, especially in mammary, muscle, and adipose tissue. Genetic deficiency of the enzyme causes familial hyperlipoproteinemia Type I. (Dorland, 27th ed) EC 3.1.1.34.
A phospholipase that hydrolyzes the acyl group attached to the 1-position of PHOSPHOGLYCERIDES.
The rate dynamics in chemical or physical systems.
Education centers authorized by the Comprehensive Health Manpower Training Act, 1971, for the training of health personnel in areas where health needs are the greatest. May be used for centers other than those established by the United States act.
Individuals enrolled in a school or formal educational program.
Schools which offer training in the area of health.
Individuals enrolled in a school of medicine or a formal educational program in medicine.
Time period from 1901 through 2000 of the common era.
Hospital department responsible for administering educational and training activities pertaining to health for patients and staff.
The mucous membrane lining of the uterine cavity that is hormonally responsive during the MENSTRUAL CYCLE and PREGNANCY. The endometrium undergoes cyclic changes that characterize MENSTRUATION. After successful FERTILIZATION, it serves to sustain the developing embryo.
Cells contained in the bone marrow including fat cells (see ADIPOCYTES); STROMAL CELLS; MEGAKARYOCYTES; and the immediate precursors of most blood cells.
Relatively undifferentiated cells that retain the ability to divide and proliferate throughout postnatal life to provide progenitor cells that can differentiate into specialized cells.
The transference of BONE MARROW from one human or animal to another for a variety of purposes including HEMATOPOIETIC STEM CELL TRANSPLANTATION or MESENCHYMAL STEM CELL TRANSPLANTATION.
The transfer of STEM CELLS from one individual to another within the same species (TRANSPLANTATION, HOMOLOGOUS) or between species (XENOTRANSPLANTATION), or transfer within the same individual (TRANSPLANTATION, AUTOLOGOUS). The source and location of the stem cells determines their potency or pluripotency to differentiate into various cell types.
Progenitor cells from which all blood cells derive.
A condition in which functional endometrial tissue is present outside the UTERUS. It is often confined to the PELVIS involving the OVARY, the ligaments, cul-de-sac, and the uterovesical peritoneum.

Metabolic engineering of a 1,2-propanediol pathway in Escherichia coli. (1/345)

1,2-Propanediol (1,2-PD) is a major commodity chemical that is currently derived from propylene, a nonrenewable resource. A goal of our research is to develop fermentation routes to 1,2-PD from renewable resources. Here we report the production of enantiomerically pure R-1,2-PD from glucose in Escherichia coli expressing NADH-linked glycerol dehydrogenase genes (E. coli gldA or Klebsiella pneumoniae dhaD). We also show that E. coli overexpressing the E. coli methylglyoxal synthase gene (mgs) produced 1,2-PD. The expression of either glycerol dehydrogenase or methylglyoxal synthase resulted in the anaerobic production of approximately 0.25 g of 1,2-PD per liter. R-1,2-PD production was further improved to 0.7 g of 1,2-PD per liter when methylglyoxal synthase and glycerol dehydrogenase (gldA) were coexpressed. In vitro studies indicated that the route to R-1,2-PD involved the reduction of methylglyoxal to R-lactaldehyde by the recombinant glycerol dehydrogenase and the reduction of R-lactaldehyde to R-1, 2-PD by a native E. coli activity. We expect that R-1,2-PD production can be significantly improved through further metabolic and bioprocess engineering.  (+info)

The pro-phenoloxidase of coleopteran insect, Tenebrio molitor, larvae was activated during cell clump/cell adhesion of insect cellular defense reactions. (2/345)

To characterize the proteins involved in cell clump/cell adhesion of insect cellular defense reactions, we induced the cell clump/cell adhesion reaction in vitro with the hemolymph of larvae of the coleopteran insect, Tenebrio molitor. The 72 kDa protein was specifically enriched in the residues of cell clump/cell adhesion and was purified to homogeneity. A cDNA clone for the 72 kDa protein was isolated. We found that the 72 kDa protein was an activated phenoloxidase from Tenebrio pro-phenoloxidase. We suggest that activated phenoloxidase is involved in the cell clump/cell adhesion reaction as well as in the synthesis of melanin.  (+info)

Effects of varying the expression level of recombinant human mGlu1alpha receptors on the pharmacological properties of agonists and antagonists. (3/345)

1. Different expression levels of the human type 1alpha metabotropic glutamate (mGlu1alpha) receptor were obtained in transfected Chinese hamster ovary cells using an isopropyl beta-D-thiogalactopyranoside (IPTG) inducible system. Expression of mGlu1alpha receptors could not be detected using immunoblotting or immunocytochemical approaches in non-induced cells, however, controlled expression could be induced following IPTG addition in a time- and concentration-dependent manner. 2. In induced cells (100 microM IPTG, 20 h) the agonists L-quisqualate or 1-aminocyclopentane-1S,3R-dicarboxylic acid stimulated large increases in [3H]-inositol (poly)phosphate (in the presence of Li+) and inositol, 1,4,5-trisphosphate levels. 3. Induction with 1-100 microM IPTG allowed the receptor density to be increased incrementally and this not only resulted in an increase in the maximum response to L-quisqualate, 1-aminocyclopentane-1S,3R-dicarboxylic acid and (S)-3,5-dihydroxy-phenylglycine, but also in an increase in the respective potencies of each agent to activate phosphoinositide hydrolysis. 4. The intrinsic activity of the partial agonist 1-aminocyclopentane-1S,3R-dicarboxylic acid dramatically increased with increasing receptor expression. 5. The activities of the competitive mGlu1alpha receptor antagonists (S)-alpha-methyl-4-carboxyphenylglycine and (S)-4-carboxy-3-hydroxyphenylglycine for inhibition of the effects of L-quisqualate or (S)-3,5-dihydroxyphenylglycine were found to be independent of the receptor expression level. 6. When the mGlu1alpha receptor was expressed at very high levels, no evidence for receptor constitutive activity could be detected, and none of the antagonists tested revealed either any intrinsic activity or negative efficacy. 7. These data demonstrate that both the potency and efficacy of mGlu1alpha receptor agonists are influenced by expression level, whilst mGlu1alpha receptor antagonist activities are independent of expression level.  (+info)

The induction of apoptosis in HeLa cells by the loss of LBP-p40. (4/345)

To analyze the function of the laminin-binding protein precursor p40 (LBP-p40) in higher eukaryotic cells, plasmid DNA expressing antisense or sense cDNA for p40 under the control of the LacSwitch system was introduced into HeLa cells. Stable transformants were isolated, and the expression of p40 was assayed by Western and Northern blotting. The expression level of p40 was not affected in HeLa cell transformants cultured in 10% serum-supplemented media with the induction of antisense (AS)-p40 with 5 mM IPTG. However, both the protein and message for endogenous p40 in serum-depleted media with 5 mM IPTG were reduced to about 30 - 10% of the expression level in serum-free media without 5 mM IPTG. Colony formation was inhibited with the suppression of p40. AS-p40 clones died in 7 days when cultured in serum-depleted media with 5 mM IPTG, while clones without 5 mM IPTG AS-p40 clones never died, even in serum-depleted media. Additionally, sense (S)-p40 clones and control CAT clones survived more than 2 weeks in serum-free media with 5 mM IPTG. DNA fragmentation assay revealed that cell death induced by the reduction of AS-p40 resulted from apoptosis. Both the inhibition of cell growth and apoptotic cell death were partially rescued by the transfer of the p40 cDNA expression vector to AS-p40 clones. Moreover, the introduction of a synthetic hammerhead ribozyme for LBP-p40 using a fusigenic viral liposome suppressed the message for LBP-p40 even in the presence of 10% serum, and it also induced apoptosis.  (+info)

Catabolite control of Escherichia coli regulatory protein BglG activity by antagonistically acting phosphorylations. (5/345)

In bacteria various sugars are taken up and concomitantly phosphorylated by sugar-specific enzymes II (EII) of the phosphoenolpyruvate:sugar phosphotransferase system (PTS). The phosphoryl groups are donated by the phosphocarrier protein HPr. BglG, the positively acting regulatory protein of the Escherichia coli bgl (beta-glucoside utilization) operon, is known to be negatively regulated by reversible phosphorylation catalyzed by the membrane spanning beta-glucoside-specific EIIBgl. Here we present evidence that in addition BglG must be phosphorylated by HPr at a distinct site to gain activity. Our data suggest that this second, shortcut route of phosphorylation is used to monitor the state of the various PTS sugar availabilities in order to hierarchically tune expression of the bgl operon in a physiologically meaningful way. Thus, the PTS may represent a highly integrated signal transduction network in carbon catabolite control.  (+info)

Escherichia coli gene ydeA encodes a major facilitator pump which exports L-arabinose and isopropyl-beta-D-thiogalactopyranoside. (6/345)

Inactivation of the Escherichia coli gene ydeA, which encodes a member of the major facilitator superfamily, decreased the efflux of L-arabinose, thereby affecting the expression of AraC-regulated genes. In addition, overexpression of ydeA decreased the expression of genes regulated by isopropyl-beta-D-thiogalactopyranoside.  (+info)

Coupling efficiencies of beta 1- and beta 2-adrenergic receptors expressed alone or together in transfected GH3 pituitary cells. (7/345)

The relationship between rat beta(1)- and beta(2)-adrenergic receptors (ARs) and cyclic AMP (cAMP) responses was examined by inducible expression of each subtype in transfected GH(3) pituitary cells. Increasing expression of beta(1)- or beta(2)-ARs in stably transfected subclones increased basal cAMP, increased the potency of isoproterenol in stimulating cAMP formation, but did not change the maximal response. A linear relationship was observed between log B(max) and -log EC(50) for isoproterenol, with no significant differences between beta(1)- and beta(2)-ARs. When both subtypes were coexpressed at different densities and ratios, pharmacological analysis showed that both selective and nonselective agonists exerted their effects at least partially through both subtypes. Either subtype alone activated a maximal response when the other subtype was blocked, indicating a complete redundancy in coupling. Agonists could activate responses through either subtype, with responses mediated primarily through the subtype where the agonist was most potent. The nonselective agonist isoproterenol had similar potencies for activating both subtypes; however, the density and ratio of subtypes affected the relative potencies of the selective agonists norepinephrine and zinterol. We conclude that beta(1)- and beta(2)-ARs have similar coupling efficiencies in GH(3) cells, these efficiencies are not altered by coexpression of another subtype, they couple redundantly to cAMP formation, and the relative densities of beta(1)- and beta(2)-ARs control the potencies of selective agonists.  (+info)

Genome-wide expression profiling in Escherichia coli K-12. (8/345)

We have established high resolution methods for global monitoring of gene expression in Escherichia coli. Hybridization of radiolabeled cDNA to spot blots on nylon membranes was compared to hybridization of fluorescently-labeled cDNA to glass microarrays for efficiency and reproducibility. A complete set of PCR primers was created for all 4290 annotated open reading frames (ORFs) from the complete genome sequence of E.coli K-12 (MG1655). Glass- and nylon-based arrays of PCR products were prepared and used to assess global changes in gene expression. Full-length coding sequences for array printing were generated by two-step PCR amplification. In this study we measured changes in RNA levels after exposure to heat shock and following treatment with isopropyl-beta-D-thiogalactopyranoside (IPTG). Both radioactive and fluorescence-based methods showed comparable results. Treatment with IPTG resulted in high level induction of the lacZYA and melAB operons. Following heat shock treatment 119 genes were shown to have significantly altered expression levels, including 35 previously uncharacterized ORFs and most genes of the heat shock stimulon. Analysis of spot intensities from hybridization to replicate arrays identified sets of genes with signals consistently above background suggesting that at least 25% of genes were expressed at detectable levels during growth in rich media.  (+info)

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Dominic-Luc Webb molmed wrote: , On Thu, 20 Dec 2001, Duncan Clark wrote: , , , ,In most of the places varying concentration of IPTG has been suggested , , ,for induction depending upon the expression system and other things. , , ,Most of these are for an low ODs like 0.5-1. , , , , , ,In case we need to work on high ODs like 50-100 or 150 would one , , , You will never get that far for the simple reason that , spectrophotometers peak out at about 3. They will give , values up to about 4, which are meaninglessly beyond , the linear range of the spectrophotometer. OD600 are , absorbance values. Yes and no. I also think bugs cannot grow at so high OD600 (at least E. coli or similar gram negative bacteria). However, its very common to get OD600 near 6 or 7, depending on the media. To measure it, the sample must be diluted in fresh media to avoid the saturation of the spectrophotometer. Usually, we dilute the culture to get a OD 600nm between 0.1 and 1.5 Your bugs will already be dying , at about ...
Author(s): Ruegg, Thomas L; Pereira, Jose H; Chen, Joseph C; DeGiovanni, Andy; Novichkov, Pavel; Mutalik, Vivek K; Tomaleri, Giovani P; Singer, Steven W; Hillson, Nathan J; Simmons, Blake A; Adams, Paul D; Thelen, Michael P | Abstract: In the original version of this Article, an incorrect URL was provided in the Data Availability Statement regarding the deposition of plasmids listed in Supplementary Table 4. The correct URL is https://public-registry.jbei.org/folders/378 . This error has been corrected in both the PDF and HTML versions of the Article.
im not sure what you mean exactly when you say question in cell disruption and making sure youre protein is as soluble as possible. usually you need to play around with IPTG concentration and try to lower the induction temp a little to try and maximise the yeild of soluble protein. i am assuming you are doing iptg induction as there arent any details ...
This page contains information on the chemical 1,3-Propanediol, 2-(hydroxymethyl)-2-isopropyl-1-methyl-, cyclic phosphate (1:1) including: 5 synonyms/identifiers.
1-(tert-butyl)-2-isopropyl-4-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)-1H-imidazole; CAS Number: 2223040-48-8; find CombiPhos Catalysts Inc-CO1H324A64E0 MSDS, related peer-reviewed papers, technical documents, similar products & more at Sigma-Aldrich
You are viewing an interactive 3D depiction of the molecule 1-isopropyl-6-methylergoline-8-carboxylic acid (C19H24N2O2) from the PQR.
6,7-dihydro-4-hydroxy-7-isopropyl-6-oxo-N-(3-(piperidin-1-yl)propyl)thieno(2,3-b)pyridine-5-carboxamide: VRX-03011 is the potassium salt; structure in first source
Structure, properties, spectra, suppliers and links for: (3S,5R,6E)-7-[3-(4-Fluorophenyl)-1-isopropyl-1H-indol-2-yl]-3,5-dihydroxy-6-heptenoate.
Structure, properties, spectra, suppliers and links for: N-[(10S,13S,20R)-3,35-Dichloro-18,21-dihydroxy-10-isopropyl-12-oxo-8,22,39-trioxa-4,11,34,38-tet.
To determine whether the mutation of Tyr515 could affect to the activity of AtSSIV, we cloned the two mutated versions into the pDEST17 vector, which allow the IPTG-inducible expression of the SSIV versions (AtSSIV-Y515F-WCTP, AtSSIV-Y515E-WCTP). In addition, this vector allows the tagging of the polypeptides expressed with a His5x tail tag at the N-terminal end of the proteins. The two AtSSIV versions were transformed into the E. coli strain BL21 (DE3) ΔglgCAP, which lacks the endogenous glycogen synthase activity. Selected clones containing the plasmids were cultivated and the expression of the AtSSIV versions were induced by IPTG. The levels of expression of the different versions were checked by immunoblot. One mL of each culture after the IPTG induction was collected, spun down in a microfuge and cells were resuspended in SDS-PAGE loading buffer and boiled for 10 min. The different samples were loaded into an SDS-PAGE gel; bands 45678were separated by electrophoresis and transferred to a ...
The old tricks could also be useful: lower the induction temperature and IPTG concentration, and shorten the induction time.... Does the degradation occur during induction or purification? Pete On Wed, 3 May 2000, Rick Thorne wrote: , Dear Eva , , All GST-fusions are different but I can offer you some guidelines which , might help you. , , First step might be to alter the strain of bacteria you are using for a , more specialized strain eg: BL21-DE3 from Stratagene (look at the web , site to learn more about these). Alternatives are available from other , companies (but I never used them). , , If this does not help, if at all practicable, make new construct/s with , smaller bits of the protein you want to use. This sometimes removes , parts of the molecule that encourage its wholesale destruction. , , regards, , , Rick , , Eva Chen wrote: , , , Is there anybody having experience on the purification of GST fusion , , proteins? , , Our lab doesnt have much experience and now there has been a ...
A model system has been developed to explore the relationship between cell cycle arrest and chemotherapeutic toxicity. An isopropyl-1-thio-β-d-galactopyranoside-inducible P16 construct was introduced stably into a melanoma cell line and used to promote G0-G1 arrest in the recipient cells. The state of arrest was reversible and did not compromise cell viability over a period of at least 7 days. Isopropyl-1-thio-β-d-galactopyranoside-treated, arrested cells were significantly more resistant to the chemotherapeutic agents methotrexate (∼50 times), vinblastine (,100 times), and cisplatin (∼10 times) compared to controls. This strategy of protection from chemotherapy exploits one of the basic genotypic differences between normal cells and tumor cells: the integrity of genetic pathways that regulate growth.. ...
Two polymorphic forms of bis[(E)-7-[4-(4-fluorophenyl)-6-isopropyl-2-[methyl(methylsulfonyl)amino]- pyrimidin-5-yl](3R,5S)-3,5-dihydroxyhept-6-enoic acid] calcium salt, processes for making them and t
This paper present ultraviolet-visible absorption spectra of imazamethabenz-methyl (IMBM) (mixture of the isomers methyl 6-[(RS)-4-isopropyl-4-methyl-5-oxo-2-imidazolin-2-yl]-m-toluate, m-imazamethabenz, and methyl 2-[(RS)-4-isopropyl-4-methyl-5-oxo-2-imidazolin-2-yl]-p-toluate, p-imazamethabenz) and the corresponding carboxylic acid, imazamethabenz-acid (IMBA). The spectral characteristics are determined as functions of the pH. The appreciable absorbance in the visible (or near-ultraviolet) region of the spectra indicates ...
The National Institute of Standards and Technology (NIST) uses its best efforts to deliver a high quality copy of the Database and to verify that the data contained therein have been selected on the basis of sound scientific judgment. However, NIST makes no warranties to that effect, and NIST shall not be liable for any damage that may result from errors or omissions in the Database ...
The National Institute of Standards and Technology (NIST) uses its best efforts to deliver a high quality copy of the Database and to verify that the data contained therein have been selected on the basis of sound scientific judgment. However, NIST makes no warranties to that effect, and NIST shall not be liable for any damage that may result from errors or omissions in the Database ...
小分子量熱休克蛋白質是植物體內種類最豐富的一群蛋白質,能與變性蛋白質結合,防止其沉澱而對細胞造成傷害。目前已知OsHSP16.9A與OsHSP18.0皆為水稻第一族群小分子量熱休克蛋白質的成員,分別位於第一和第三對染色體上,皆會在高溫41℃ ...
Gentaur molecular products has all kinds of products like :search , PhytoTechnology Laboratories \ IPTG \ I373-1G for more molecular products just contact us
polb cloned in pET not expressed - posted in Protein Expression and Purification: I have cloned a mutant human polb sequence in a pET28a vector. I have sequenced the clone, the sequence is in reading frame. When I try to expresses the protein in BL21 with IPTG, get no product. I have tried for several time, also in different concentration of IPTG, time period and temperature. Please help me.
Magenta-Gal is an X-Gal alternative that is used with IPTG to select successfully transformed cells. Magenta-Gal with IPTG is used in red-white screening.
TY - JOUR. T1 - Composite nanomaterials by self-assembly and controlled crystallization of poly(2-isopropyl-2-oxazoline)-grafted polysaccharides. AU - Morimoto, Nobuyuki. AU - Obeid, Rodolphe. AU - Yamane, Setsuko. AU - Winnik, Franoise M.. AU - Akiyoshi, Kazunari. PY - 2009/4/20. Y1 - 2009/4/20. N2 - We report here new composite nanomaterials by self-assembly and control of crystallization of thermoresponsive poly(2-isopropyl-2-oxazoline) (PIPOZ)-grafted pullulan.. AB - We report here new composite nanomaterials by self-assembly and control of crystallization of thermoresponsive poly(2-isopropyl-2-oxazoline) (PIPOZ)-grafted pullulan.. UR - http://www.scopus.com/inward/record.url?scp=64549101215&partnerID=8YFLogxK. UR - http://www.scopus.com/inward/citedby.url?scp=64549101215&partnerID=8YFLogxK. U2 - 10.1039/b817603e. DO - 10.1039/b817603e. M3 - Article. AN - SCOPUS:64549101215. VL - 5. SP - 1597. EP - 1600. JO - Soft Matter. JF - Soft Matter. SN - 1744-683X. IS - 8. ER - ...
It is shown that the 3-benzoyl-2-isopropyl-4-alkyloxazolidin-5-ones (12), derived from isobutyraldehyde and ?-amino acids are as efficient as, but more economical than, the corresponding tert-butyl compounds (9), as sources of enantiopure ?,?-dialkylated ?-amino acids (e.g., 21-23). In the absence of alkylating agents, the anions of (2R,4S)-12 and its enantiomer undergo a fragmentation-recombination process to generate (1S,2R,4S)-N-[1-(3-benzoyl- 2-isopropyl-4-methyl-5-oxo-oxazolidin-4-yl)-2-methylpropyl]benzamide ((1S, 2R,4S)-20), and its enantiomer. Acidic methanolysis of these condensation products provides access to ?,?-dialkylated ?,?-diaminopropionic acids [e.g., (2S,3S)-2,3-bisbenzoylamino-2,4-dimethylpentanoic acid methyl ester ((2S,3S)-24)). ...
Results. Metabolizable permissive glycan supports outer segment assembly: quantification of effect. Figure 1 illustrates examples of intact retinas that were removed from stage 33/34 Xenopus laevis tadpoles and placed into culture in Niu-Twitty medium for three days. Using this paradigm, all outer segment material is elaborated while in culture [29]. In retinas that were maintained with a normally apposed RPE, the outer segments are tightly stacked, properly folded and contain discs of equal diameter (Figure 1A). This morphology is identical to retinas maturing in vivo [29]. In RPE-deprived retinas that were otherwise similarly maintained, photoreceptor outer segment membranes are markedly disorganized, with little evidence of normal disc stacking (Figure 1B). The addition of 5x10-3 M mannose did not influence favorably the folding of outer segments in RPE-deprived retinas (Figure 1C), whereas the addition of 5x10-3 M lactose (Figure 1D) supported nicely the formation of nascent outer segments ...
Results show that in this condition both BBa_R0010 and BBa_R0011 produce different amounts of RFP as a function of the IPTG concentration. The amplitude of the two curves show that the promoters are very strong when induced with IPTG ,= 10 uM. Although the experiments were carried out in the same conditions, the variability between experiments was high, especially for BBa_R0010 (mean coefficient of variaton of about 37% for BBa_R0010 and 15% for BBa_R0011), while the RPU variability between three wells in the same experiment is much lower (mean coefficient of variaton of bout 3.5% for both promoters). The above figure shows that BBa_R0011 is stronger than the BBa_R0010 wild type promoter in low copy plasmid. This result is unexpected because the same promoters in high copy vectors behaved differently (BBa_R0010 was stronger than the BBa_R0011, see above). In the uninduced state, BBa_R0011 has about the same strength as the BBa_J23101 reference standard promoter. This static characteristic shows ...
2-isopropyl-5,5-dimethyl-5,6-dihydro-2H-1,3-oxazine - chemical structural formula, chemical names, chemical properties, synthesis references
4-chloro-2-isopropyl-6-methylpyrimidine 1-oxide - chemical structural formula, chemical names, chemical properties, synthesis references
6-{[2-(2-Isopropyl-4-methylphenoxy)ethyl]thio}-9H-purine | C17H20N4OS | CID 2257073 - structure, chemical names, physical and chemical properties, classification, patents, literature, biological activities, safety/hazards/toxicity information, supplier lists, and more.
40853-56-3 - AOWLXZIJUIFJPM-KPKJPENVSA-N - Acetic acid, 2-isopropyl-5-methyl-2-hexen-1-yl ester - Similar structures search, synonyms, formulas, resource links, and other chemical information.
10-isopropyl-2,2,6-trimethyl-2,3,4,5-tetrahydronaphtha(1,8-bc)oxocine-5,11-diol: from the roots and rhizomes of Nardostachys chinensis; structure in first source
The use of λ repressor fusions to study protein-protein interactions in E. coli was first described by Hu and others (1). Since then, the repressor system has been employed by several laboratories to...
Global transcription responses of Escherichia coli to various stimuli or genetic defects were studied by measuring mRNA levels in about 400 segments of the genome. Measuring mRNA levels was done by analyzing hybridization to DNA dot blots made with overlapping lambda clones spanning the genome of E. coli K-12. Conditions examined included isopropyl-beta-D-thiogalactopyranoside (IPTG) induction, heat shock, osmotic shock, starvation for various nutrients, entrance of cells into the stationary phase of growth, anaerobic growth in a tube, growth in the gnotobiotic mouse gut, and effects of pleiotropic mutations rpoH, himA, topA, and crp. Most mapped genes known to be regulated by a particular situation were successfully detected. In addition, many chromosomal regions containing no previously known regulated genes were discovered that responded to various stimuli. This new method for studying globally regulated genetic systems in E. coli combines detection, cloning, and physical mapping of a battery ...
Inducible expression systems are frequently used for the production of heterologous proteins. Achieving maximum product concentrations requires induction profiling, namely the optimization of induction time and inducer concentration. However, the respective experiments can be very laborious and time-consuming. In this work, a new approach for induction profiling is presented where induction in a microtiter plate based cultivation system (BioLector) is achieved by light using photocaged isopropyl β-d-1-thiogalactopyranoside (cIPTG). A flavin mononucleotide-based fluorescent reporter protein (FbFP) was expressed using a T7-RNA-polymerase dependent E. coli expression system which required IPTG as inducer. High power UV-A irradiation was directed into a microtiter plate by light-emitting diodes placed above each well of a 48-well plate. Upon UV irradiation, IPTG is released (uncaged) and induces product formation. IPTG uncaging, formation of the fluorescent reporter protein and biomass growth were
For years I have been teaching my students that a gene is a segment of DNA that codes for a single RNA molecule with a complementary sequence, regardless of whether that RNA molecule is translated or not. This definition takes into account the genes for the various rRNAs and tRNAs, which are not translated, and also other forms of non-translated RNA that have recently been discovered. By this definition, genes that code for mRNAs that are actually translated are distinguished as structural genes, using terminology that was first developed to describe the Jacob-Monod model of the lactose operon. Using this same terminology, the gene that codes for the lactose repressor protein is a regulatory gene, insofar as the repressor does not function in an extrinsic biochemical pathway, but rather participates in the regulation of other structural genes. However, the distinction between structural and regulatory genes outlined above is insufficient to describe the various kinds of genetically ...
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice ...
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Mouse polyclonal antibody raised against a partial recombinant CHERP. CHERP (AAH21294.1, 795 a.a. ~ 883 a.a) partial recombinant protein with GST tag. (H00010523-A01) - Products - Abnova
Nitrogen › N-[(1R,2R)-2-(Dimethylamino)-1,2-diphenylethyl]-N-[[(1R,4aS,10aR)-1,2,3,4,4a,9,10,10a-octahydro-1,4a-dimethyl-7-isopropyl-1-phenanthrenyl]methyl]thiourea, 98%, (99% ee) ...
5-(4-ethylpiperazin-1-ylmethyl)pyridin-2-yl)-(5-fluoro-4-(7-fluoro-3-isopropyl-2-methyl-3H-benzimidazol-5-yl)pyrimidin-2-yl)amine ...
Molecular Cloning, also known as Maniatis, has served as the foundation of technical expertise in labs worldwide for 30 years. No other manual has been so popular, or so influential.
Molecular Cloning, also known as Maniatis, has served as the foundation of technical expertise in labs worldwide for 30 years. No other manual has been so popular, or so influential.
4-ISOPROPYL-2-PHENYL-2-OXAZOLINE-5-ONE (CAS 5839-93-0) Market Research Report 2018 aims at providing comprehensive data on 4-isopropyl-2-phenyl-2-oxazoline-5-one
This page contains information on the chemical Barbituric acid, 5-(2-bromoallyl)-5-isopropyl-1-methyl-, sodium salt including: 11 synonyms/identifiers.
64038-35-3 - ZOKRAMSWASSLAJ-IPZCTEOASA-M - Barbituric acid, 5-isopropyl-5-(2-pentenyl)-, sodium salt - Similar structures search, synonyms, formulas, resource links, and other chemical information.
View Notes - 351 E2p mc from CHEM 351 at BYU. 351 E2 Practice 1. How many stereoisomers are there of 1-isopropyl-4-methylcyclohexane? A. only 1 structure possible - no stereoisomers B. two C. three
This research project attempted to grow and purify soluble Pseudomonas putida HMGR (PpHMGR) enzyme for the purpose of experimentation on its hypothesized morpheein protein behavior. Inclusion body formation has been the primary limiting factor in the growth and purification of soluble PpHMGR. Variation on incubation temperature, IPTG concentration, and growth time were explored in the growth phase, and imidazole concentration, presence of DTT, and presence of TCEP were explored in the purification phase. PpHMGR was grown in an E. coli host, cell lysis was performed by sonication, and subsequent purification was performed by nickel column elution FPLC. The purified product was characterized by FPLC, gel electrophoresis, and enzyme kinetics to determine the reduction of inclusion body formation and presence of soluble PpHMGR.
Supplementary MaterialsAdditional file 1: Figure S1. expression of the TetR family protein when induced with different inducer concentrations. Lane1: marker, lane2: cells with uninduced TetR family protein, lane3-6: cells with induced TetR family protein (0.2?mM, 0.5?mM, 1?mM and 2?mM IPTG concentration). 13568_2019_801_MOESM1_ESM.pdf (426K) GUID:?E4D189AF-6495-44A5-9CF1-3575D2773CCA Data Availability StatementAll data analyzed throughout this Mouse monoclonal to EhpB1 study is shown in the article. Abstract Biodesulfurization helps in removal of sulfur from organosulfur present in petroleum fractions. All microorganisms isolated to date harbor a desulfurization operon consisting of three genes and -which encode for monooxygenases (DszA & C) and desulfinase (DszB). Most of the studies have been carried out using dibenzothiophene as the model organosulfur compound, which is converted into 2 hydroxybiphenyl by a 4S pathway which maintains the calorific value of fuel. There are few studies reported ...
A putative operon encoding a probable zinc-responsive regulatory element (zur) and components of an ABC-type transporter (mreA mreB) have been characterized in Staphylococcus aureus. The zur gene was inactivated but apparently this did not alter Zn2+ uptake. Expression of mreAB zur is at a low level under a range of ion conditions. To allow inducible expression of the operon, a construct was made placing it under the control of the IPTG-inducible Pspac promoter. Using this approach, it was shown that zur is able to repress expression of the entire operon in a Zn2+-dependent manner, and that mreA and mreB are likely to be involved in high-affinity ion uptake. zur has no apparent role in pathogenicity in a lesion model of S. aureus infection.
The Argonaute protein of Thermus thermophilus (TtAgo) has recently been studied in detail. For its in vitro characterization, TtAgo was purified after heterologous expression in Escherichia coli (E. coli). As TtAgo expression is toxic, a tightly controlled system was used for protein expression. The expression strain E. coli KRX carries a chromosomal T7 RNA polymerase gene under control of a rhamnose promoter. The ago gene is expressed via an IPTG-inducible T7 promoter. This allows for tightly (double) controlled expression of (toxic) TtAgo. Here, we describe the steps required for controlled expression and purification of this toxic protein.
More specifically: The bacterial cells of strain HTll5(DE3) contain the T7 RNA polymerase gene (contained within a stable insertion of a modified lambda prophage λ DE3) under the control of lac operon regulatory elements. This allows expression of T7 polymerase to be controlled by isopropyl-β-D-thiogalactopyranoside (IPTG), a lactose analogue that induces expression of genes under the control of the lac operon o gene. When IPTG is added, the cells will begin to synthesize lots of T7 RNA polymerase. This T7 RNA polymerase can then bind to the T7 promoter sites on the plasmid and begin to synthesize RNA from both T7 RNA polymerase sites. Because the two single strands of RNA are complementary to each other they will form double stranded RNA within the bacterial cell. The IPTG induction allows us to turn on and express the plasmid gene of interest only when we want to and it allow us to make much higher levels of RNA for RNA interference than would be made without this induction. Another useful ...
More specifically: The bacterial cells of strain HTll5(DE3) contain the T7 RNA polymerase gene (contained within a stable insertion of a modified lambda prophage λ DE3) under the control of lac operon regulatory elements. This allows expression of T7 polymerase to be controlled by isopropyl-β-D-thiogalactopyranoside (IPTG), a lactose analogue that induces expression of genes under the control of the lac operon o gene. When IPTG is added, the cells will begin to synthesize lots of T7 RNA polymerase. This T7 RNA polymerase can then bind to the T7 promoter sites on the plasmid and begin to synthesize RNA from both T7 RNA polymerase sites. Because the two single strands of RNA are complementary to each other they will form double stranded RNA within the bacterial cell. The IPTG induction allows us to turn on and express the plasmid gene of interest only when we want to and it allow us to make much higher levels of RNA for RNA interference than would be made without this induction. Another useful ...
When using this server please cite the following paper:. Zsila F, Bikadi Z, Malik D, Hari P, Pechan I, Berces A, Hazai E.. Evaluation of drug-human serum albumin binding interactions with support vector machine aided online automated docking.. Bioinformatics. 2011 May 18. ...
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Chemical Entities of Biological Interest (ChEBI) is a freely available dictionary of molecular entities focused on small chemical compounds.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice ...
The RE family based on the Silicon on Thin Buried Oxide (SOTB™ ) process technology realizes both ultra-low current consumption in both active and standby mode and high speed CPU operation (64MHz) at low voltage (1.62V) , which is impossible to achieve wi...
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The present invention relates to 4-{(1R,3R)-1-(3,5-difluorophenyl)-3-[4-(3-ethyl-5-isopropyl-4H-1,2,4-triazol-4-yl)piperidin-1-yl]butyl}-1-(methylsulfonyl)piperidine (I): |p||chemistry id=CHEM-US-000
Torsemide is a diuretic of the pyridine-sulfonylurea class. Its chemical name is 1-isopropyl-3-[(4-m-toluidino-3-pyridyl) sulfonyl] urea. Torsemide acts from within the lumen of the thick ascending portion of the loop of Henle, where it inhibits the Na/K+/2CI- carrier system. ...
[QUOTE=robal]OMG Elvish, what was this, this is so hot...how hot is it going to be on their wedding night, cant wait to read that. I have been waiting impatiently to read the next chapter of soulmates and let me tell you, I thoroughly enjoyed it. It was worth the wait. I hope you update the ... | Page: 38 | 4610380 | Meri Aashiqui Tum Se Hi Forum
Both hybrid promoters can be repressed by the lac repressor and both can be derepressed with isopropyl-beta-D-thiogalactoside. ... Isopropyl β-D-1-thiogalactopyranoside), whilst also allowing higher maximum gene expression than either the lac or trp ...
... recombinant is then transformed into XL1-Blue cells and expression is induced by the addition of isopropyl β-D-thiogalactoside ...
... thiogalactosides MeSH D09.408.320.820.500 - isopropyl thiogalactoside MeSH D09.408.348.050 - amygdalin MeSH D09.408.348.075 - ... thiogalactosides MeSH D09.408.903.600.500 - isopropyl thiogalactoside MeSH D09.408.903.703 - thioglucosides MeSH D09.408. ...
Example of a substrate analog that is also a gratuitous inducer: IPTG (isopropyl β-thiogalactoside: substrate analog and ...
Beta-galactosidase inducer.Isopropyl-β-D-thiogalactoside is used as a reagent in molecular biology. It is used as an effective ...
IPTG is primarily used in protein induction. IPTG permits the synthesis of ß-galactosidase, and its used with X-Gal or Bluo-Gal for colony screening.
Isopropyl β-D-Thiogalactoside. LB. Luria-Bertani. MHB. Müller-Hinton broth. Mxe. Mxe GyrA intein ... E. coli BL21(DE3) cells harboring plasmid pET21a-AM-His were cultured at 37 °C and isopropyl β-D-1-thiogalactopyranoside (IPTG ...
The pGFP-EpsL plasmid was constructed by cloning the gfp fragment into the low-copy isopropyl β-d-thiogalactoside (IPTG)- ...
... the tac promoter was induced by the addition of 1 mM isopropyl-β-d-thiogalactoside (IPTG) 30 min after the temperature shift. ...
isopropyl β-d-thiogalactoside. HPLC. high performance liquid chromatography. PPP. pentose phosphate pathway ... all the recombinant proteins expression were induced by 100 μM isopropyl-β-d-thiogalactopyranoside (IPTG) for T7 promoter and ...
Lysates were obtained from E. coli (DE3) cells with pET30a-FIP-fve induced by 1 mM Isopropyl β-d-thiogalactoside (IPTG) and ... Lysates were obtained from E. coli (DE3) cells with pET30a-FIP-fve induced by 1 mM Isopropyl β-d-thiogalactoside (IPTG) and ... The converted E. coli cells of DE3 cells with pET30a-FIP-fve plasmid were incubated and induced with 1 mM isopropyl β-d- ... thiogalactoside (IPTG; TaKaRa) upon reaching 0.8 OD600. The applied time course was chosen at 1.5, 3.0, and 4.5 h after IPTG ...
Isopropyl Thiogalactoside / pharmacology * Models, Genetic * Nucleotides / metabolism * Promoter Regions, Genetic* * Rifampin ...
Isopropyl Thiogalactoside * calyculin A * Protein-Serine-Threonine Kinases * rho-Associated Kinases * Phosphoprotein ...
Isopropyl thiogalactoside View Synonyms. View Structure. Description:. A non-metabolizable galactose analog that induces ...
Isopropyl β-D-thiogalactoside; Linear Formula: C9H18O5S; find Sigma-Aldrich-I5502 MSDS, related peer-reviewed papers, technical ... Isopropyl β-D-1-thiogalactopyranoside ≥99% (TLC); CAS Number: 367-93-1; EC Number: 206-703-0; Synonym: IPTG, ... Isopropyl β-. D. -1-thiogalactopyranoside ≥99% (TLC) Synonym: IPTG, Isopropyl β-. D. -thiogalactoside ...
... isopropyl-β-d-thiogalactoside; GPE1, G-CSF gene promoter element 1; BCL, B cell leukemia. ... The cells were split into two equal parts posttransfection and cultured in the presence (1 mM) or absence of isopropyl-β-d- ... thiogalactoside (IPTG). NF-IL6 M1 cells inducibly expressed human NF-IL6 protein within 4 h after IPTG addition as described ...
... isopropyl β-d-thiogalactoside; TCA; trichloroacetic acid; MIDA, mass isotopomer distribution analysis; CIEF, capillary ...
IPTG (Isopropyl-β-D-thiogalactoside). (Catalog Number I6758). 15ml polypropylene culture tubes (sterile). ... IPTG (Isopropyl-β-D-thiogalactoside) (Catalog Number I6758). 15ml polypropylene culture tubes (sterile). ...
... transformed Escherichia coli that were stimulated with isopropyl β-d-thiogalactoside (IPTG) (14). The ICAM-1 derivative ...
The expression of BbPlgl was induced by addition of IPTG (isopropyl β-D-thiogalactoside) to the culture at a final ...
Isopropyl β-D-1-thiogalactopyranoside, Isopropyl beta-D-1-thiogalactopyranoside, 367-93-1. ... IPTG(Isopropyl ?-D-thiogalactoside) is a molecular mimic of allolactose, a lactose metabolite that triggers transcription of ... actoside; isopropyl. -??-D-thiogalactopy. ranoside; isopropyl. -??-D-thiogalactosi. de isopropyl ??-d-1-th. iogalactopyranoside ... Isopropyl-β-D-thiog. alactopyranoside, &. lt;5ppm dioxane Isopropyl-β-D-thiog. alactopyranoside, &. lt;5ppm dioxane, pl. ant ...
Isopropyl β-D-thiogalactoside (IPTG; EMD Millipore, Billerica, MA; 1 mM) was added to induce protein expression, and after 4 h ...
Isopropyl beta-D-thiogalactoside(IPTG)是异乳 ... Isopropyl beta-D-thiogalactoside,CAS:367-93-1,分子式:C9H18O5S,分子量: ... Isopropyl beta-D-thiogalactoside(IPTG)是异乳糖模拟物,能够引起乳糖操纵子的转录过程,因此能够诱导乳糖操纵子下游基因对应蛋白的表达。 ... 英文名
in the presence of isopropyl β-D-thiogalactoside (IPTG, 1 mM final concentration). A pellet expressing cells was suspended in ...
Isopropyl-beta-D-thiogalactoside, dioxane-free, 99%. * 10210. Holmium foil, 0.3mm (0.01in) thick, 99.9% (REO) ...
isopropyl-β-D-thiogalactoside (IPTG). Invitrogen. 15529-019. Bacterial growth. Diethanolamine. Sigma. D-8885. Enzymatic assays ... Follow growth of cultures and at an OD620 = 0.3, add isopropyl-β-D-thiogalactoside (IPTG) to a final concentration of 100 µM, ...
isopropyl-β-D-thiogalactoside (IPTG). Invitrogen. 15529-019. Bacterial growth. Diethanolamine. Sigma. D-8885. Enzymatic assays ...
Isopropyl‐β‐D‐thiogalactoside (IPTG), a genetic switch. (a) When IPTG is not present in the growth medium of the cell culture ...
Isopropyl thiogalactoside is. a) An analog of lactose. b) Gratuitous inducer. c) Both the above √ ...
These data were confirmed with isopropyl β-d-thiogalactoside staining as demonstrated in Figure 4. After ten weeks, ...
On the Enzymic Acetylation of Isopropyl-β-D-Thiogalactoside and its Association with Galactoside-Permease. Lopéron: Groupe de ... Thiogalactoside Transacetylase. Sur la Nature du Répresseur Assurant limmunité des Bactéries Lysogènes. [On the Nature of the ...
isopropyl-β-D-thiogalactoside. Footnotes. Conflict of Interest statement: The authors declare that there are no conflicts of ...
... and cells at the exponential stage were induced with 0.5 mM isopropyl β-D-thiogalactoside (IPTG). The Trx-tagged proteins were ...
Both hybrid promoters can be repressed by the lac repressor and both can be derepressed with isopropyl-beta-D-thiogalactoside. ... Isopropyl β-D-1-thiogalactopyranoside), whilst also allowing higher maximum gene expression than either the lac or trp ...
  • The isopropyl-AY-D-thiogalactopyranoside ( IPTG ) is commonly used inducer to regulate the expression of target proteins under the influence this promoter in BL21 (DE3) strain. (thefreedictionary.com)
  • Later, the transformed cell culture was plated on LB medium supplemented (100 g/mL) ampicillin, X-gal 5-bromo-4- chloro-3-indolyl-AY-D-galactosidase (40 ng/mL) and IPTG isopropyl-AY-Dthiogalactopy-ranoside (0.5 mM) and incubated over night at 37C. (thefreedictionary.com)
  • expression was then induced with different concentrations of isopropyl β-D-thiogalactoside (IPTG). (amrita.edu)
  • The tac promoter is, therefore, inducible by IPTG (Isopropyl β-D-1-thiogalactopyranoside), whilst also allowing higher maximum gene expression than either the lac or trp promoters. (wikipedia.org)
  • The vectors are isopropyl-β- d -thiogalactoside (IPTG)-inducible, allowing for the conditional silencing of target genes. (asm.org)
  • Isopropyl-β-D-thiogalactoside (IPTG ) - is an inductor but not a substrate for β-galactosidase. (biology-online.org)
  • The minimum quantity of (isopropyl--D-thiogalactoside) IPTG for optimal induction was estimated in 18-20 ºmol IPTG gDCW-1. (csic.es)
  • In cells coinfected with VV recombinants and VSV, induction with isopropyl-B-D-thiogalactoside (IPTG) of wild-type p68 kinase or a mutant lacking the dsRNA binding domain resulted in inhibition of both VV and VSV protein synthesis. (nih.gov)
  • If we take a small sample from our mixed soup of bacteria and spread it out on IPTG (isopropyl beta-D-thiogalactoside) Agar plates (round plates on which bacteria like to grow), this will spread out the bacteria over a large area. (google.com)
  • We determined the three-dimensional structures of the intact lac repressor, the lac repressor bound to the gratuitous inducer 1-isopropyl-b-D-thiogalactoside (IPTG) and the lac repressor complexed with a 21 base-pair symmetric operator DNA. (upenn.edu)
  • Both hybrid promoters can be repressed by the lac repressor and both can be derepressed with isopropyl-beta-D-thiogalactoside. (wikipedia.org)
  • Escherichia coli was transformed with the expression vectors and enzymatically active soluble proteins were obtained after induction with isopropyl thio-beta-d-thiogalactoside. (lu.se)
  • Beta-galactosidase inducer.Isopropyl-β-D-thiogalactoside is used as a reagent in molecular biology. (alfa.com)
  • Lactose could be used as an alternative inducer to isopropyl-β-D-thiogalactoside, thus reducing the production cost. (avhandlingar.se)
  • The reporter gene can be specifically activated by administration of the lactose analogue isopropyl β- d -thiogalactoside. (aacrjournals.org)
  • Inhibition de l'adaptation Enzymatique chez B. Coli en Présence de 2-4 Dinitrophénol. (elsevier.com)