Thyroxine-Binding Proteins
Serum Globulins
Thyroxine-Binding Globulin
Click Chemistry
Thyroxine
Differential expression and phosphorylation of CTCF, a c-myc transcriptional regulator, during differentiation of human myeloid cells. (1/3619)
CTCF is a transcriptional repressor of the c-myc gene. Although CTCF has been characterized in some detail, there is very little information about the regulation of CTCF activity. Therefore we investigated CTCF expression and phosphorylation during induced differentiation of human myeloid leukemia cells. We found that: (i) both CTCF mRNA and protein are down-regulated during terminal differentiation in most cell lines tested; (ii) CTCF down-regulation is retarded and less pronounced than that of c-myc; (iii) CTCF protein is differentially phosphorylated and the phosphorylation profiles depend on the differentiation pathway. We concluded that CTCF expression and activity is controlled at transcriptional and post-transcriptional levels. (+info)Human triclonal anti-IgG gammopathy. I. Iso-electric focusing characteristics of the IgG, IgA and IgM anti-IgG and their heavy and light chains. (2/3619)
Human IgG, IgA and IgM anti-IgG autoantibodies have been isolated from the serum of an individual with Felty's syndrome. These were initially noted as soluble circulating serum complexes by analytical ultracentrifugation. Isolation was accomplished by solid phase immunoadsorption and each of the three antibody populations obtained was shown to be of restricted heterogeneity by liquid and polyacrylamide gel electrofocussing methods. Type kappa light chains were obtained from each protein. Co-isoelectric focusing experiments of all possible pairs of these light chains showed them to have identical net charge characteristics. Heavy chains obtained from each protein were also monoclonal and of differing isoelectric point. The availability of this serum provides a human model with which to study the changes which may occur in autoantibodies during the autoimmune response. (+info)Molecular and biochemical characterization of VEB-1, a novel class A extended-spectrum beta-lactamase encoded by an Escherichia coli integron gene. (3/3619)
A clinical isolate, Escherichia coli MG-1, isolated from a 4-month-old Vietnamese orphan child, produced a beta-lactamase conferring resistance to extended-spectrum cephalosporins and aztreonam. In a disk diffusion test, a typical synergistic effect between ceftazidime or aztreonam and clavulanic acid was observed along with an unusual synergy between cefoxitin and cefuroxime. The gene for VEB-1 (Vietnamese extended-spectrum beta-lactamase) was cloned and expressed in E. coli JM109. The recombinant plasmid pRLT1 produced a beta-lactamase with a pI of 5.35 and conferred high-level resistance to extended-spectrum (or oxyimino) cephalosporins and to aztreonam. Vmax values for extended-spectrum cephalosporins were uncommonly high, while the affinity of the enzyme for ceftazidime and aztreonam was relatively low. blaVEB-1 showed significant homology at the DNA level with only blaPER-1 and blaPER-2. Analysis of the deduced protein sequence showed that VEB-1 is a class A penicillinase having very low levels of homology with any other known beta-lactamases. The highest percentage of amino acid identity was 38% with PER-1 or PER-2, two uncommon class A extended-spectrum enzymes. Exploration of the genetic environment of blaVEB-1 revealed the presence of gene cassette features, i.e., (i) a 59-base element associated with blaVEB-1; (ii) a second 59-base element just upstream of blaVEB-1, likely belonging to the aacA1-orfG gene cassette; (iii) two core sites (GTTRRRY) on both sides of blaVEB-1; and (iv) a second antibiotic resistance gene 3' of blaVEB-1, aadB. blaVEB-1 may therefore be the first class A extended-spectrum beta-lactamase that is part of a gene cassette, which itself is likely to be located on a class 1 integron, as sulfamide resistance may indicate. Furthermore, blaVEB-1 is encoded on a large (> 100-kb) transferable plasmid found in a Klebsiella pneumoniae MG-2 isolated at the same time from the same patient, indicating a horizontal gene transfer. (+info)Mechanisms of beta-lactam resistance amongst Pseudomonas aeruginosa isolated in an Italian survey. (4/3619)
The mechanisms of resistance to beta-lactam antibiotics in 325 isolates of Pseudomonas aeruginosa were examined. These isolates were selected because of their resistance to meropenem and imipenem (breakpoint, >4 mg/L), carbenicillin (>128 mg/L), ceftazidime (>8 mg/L), piperacillin and ticarcillin/clavulanate (>64 mg/L). The most frequent mechanism of resistance was beta-lactamase-independent, so called 'intrinsic resistance', which was found in 183 isolates and was probably due to impermeability and/or efflux mechanisms. beta-Lactamase-mediated resistance was demonstrated in 111 strains (11.1%). Derepression of Ambler Class C chromosomal beta-lactamase was detected in 64 isolates, most of which were resistant to ceftazidime and piperacillin but susceptible to meropenem, whereas secondary plasmid-encoded beta-lactamases were found in 34 isolates, all of them resistant to carboxypenicillins and ureidopenicillins and susceptible to carbapenems. Twelve strains showed more than one plasmid-encoded beta-lactamase plus derepression of chromosomal Class C enzyme. Resistance to carbapenems was independent of resistance to other beta-lactam antibiotics, indicating a different mechanism of resistance, probably due to the loss of the D2 porin. In total, 32 strains were resistant to carbapenems: 24 only to imipenem and eight to both imipenem and meropenem. (+info)Differential serodiagnosis for cystic and alveolar echinococcosis using fractions of Echinococcus granulosus cyst fluid (antigen B) and E. multilocularis protoscolex (EM18). (5/3619)
Echinococcus granulosus cyst fluid and E. multilocularis protoscolex extract were fractionated by a single step of preparative isoelectric focusing, resulting in an antigen B-rich fraction (8-kD) and an Em18-rich fraction, respectively. The usefulness of both fractions for differential serodiagnosis of cystic (CE) and alveolar (AE) echinococcosis was evaluated by a large-scale immunoblot analysis on a battery of 354 serum samples. These included 66 from AE patients originating from four different endemic areas, 173 from CE patients originating from seven different endemic areas, 71 from patients with other parasitic diseases, 15 from patients with hepatomas, and 29 from healthy individuals. In an immunoblot with the antigen B-rich fraction, 92% (158 of 173) of the CE sera as well as 79% (52 of 66) of the AE sera reacted with the 8-kD subunit. No cross-reactivity occurred with any sera from patients with cysticercosis, other parasitic diseases, or with hepatomas, or from healthy controls. In an immunoblot with the Em18-rich fraction, all but two sera from AE patients (64 of 66, 97%) recognized Em18, and only nine of 34 CE sera from China reacted with it. All other (139) CE sera from six other countries were negative as were all (115) other non-echinococcosis sera. These findings indicate that antigen B (8-kD) is not species-specific for E. granulosus but is genus-specific for Echinococcus, and that the Em18 antigen is a reliable serologic marker for species-specific differentiation of AE from CE. (+info)The relationship of glycosylation and isoelectric point with tumor accumulation of avidin. (6/3619)
Radiolabeled avidin markedly accumulated in intraperitoneal tumors and was cleared rapidly from circulation when given intraperitoneally. This study investigated the mechanisms of the tumor localization of avidin. METHODS: Avidin was deglycosylated through endoglycosydase-H digestion and/or neutralized by acetylation of its lysine amino acids with acetic acid N-hydroxysuccinimide ester. Avidin and modified avidins were analyzed using sodium dodecylsulfate-polyacrylamide gel electrophoresis (SDS/PAGE) and isoelectric focusing. A tumor model was established by intraperitoneal injection of human colon cancer cells, LS180, in nude mice. Avidin and modified avidins were labeled with 111In using diethyleneamine pentaacetic acid-biotin and were administered intraperitoneally into the tumor-bearing mice. The biodistribution of radioactivity was examined 2 and 24 h postinjection. RESULTS: Deglycosylated avidins revealed a major band of smaller molecules on SDS/PAGE. The isoelectric point of neutralized avidins was reduced to less than 5, whereas that of unneutralized avidins was more than 9.5. Biodistribution study demonstrated that liver uptake was decreased by deglycosylation and kidney accumulation was decreased by neutralization, respectively. The blood clearance was remarkably slowed by combined modification of deglycosylation and neutralization. The tumor uptake of radioactivity was reduced by either deglycosylation or neutralization and was further decreased with combined modification. CONCLUSION: Both high glycosylation and positive charge of avidin contributed to its accumulation in tumor. This study may facilitate development of a new vehicle for the delivery of therapeutic agents to intraperitoneal tumors. (+info)Isolation of pigment-binding early light-inducible proteins from pea. (7/3619)
The early light-inducible proteins (ELIPs) in chloroplasts possess a high sequence homology with the chlorophyll a/b-binding proteins but differ from those proteins by their substoichiometric and transient appearance. In the present study ELIPs of pea were isolated by a two-step purification strategy: perfusion chromatography in combination with preparative isoelectric focussing. Two heterogeneous populations of ELIPs were obtained after chromatographic separation of solubilized thylakoid membranes using a weak anion exchange column. One of these populations contained ELIPs in a free form providing the first isolation of these proteins. To prove whether the isolated and pure forms of ELIP bind pigments, spectroscopic and chromatographic analysis were performed. Absorption spectra and TLC revealed the presence of chlorophyll a and lutein. Measurements of steady-state fluorescence emission spectra at 77 K exhibited a major peak at 674 nm typical for chlorophyll a bound to the protein matrix. The action spectrum of the fluorescence emission measured at 674 nm showed several peaks originating mainly from chlorophyll a. It is proposed that ELIPs are transient chlorophyll-binding proteins not involved in light-harvesting but functioning as scavengers for chlorophyll molecules during turnover of pigment-binding proteins. (+info)Low-temperature sensitivity and enhanced Bohr effect in red deer (Cervus elaphus) haemoglobin: a molecular adaptive strategy to life at high altitude and low temperature. (8/3619)
A study of the functional properties of haemoglobin from red deer (Cervus elaphus) whose habitat varies over a wide range of latitude, was performed. The oxygen-binding properties of the most common haemoglobin phenotype from the species living in Sardinia were examined with particular attention to the effect of pH, chloride, 2, 3-bisphosphoglycerate and temperature. Results indicate that red deer haemoglobin, like all haemoglobins from ruminants so far examined, is characterized by a low intrinsic oxygen affinity, with chloride being its main physiological modulator in vivo. The functional results and the low temperature sensitivity of the oxygen affinity are discussed in the light of the amino acid sequence of closely related ruminant haemoglobins. (+info)Thyroxine-binding proteins (TBPs) are specialized transport proteins in the blood that bind and carry thyroid hormones, primarily Thyroxine (T4), but also Triiodothyronine (T3) to a lesser extent. The majority of T4 and T3 in the blood are bound to these proteins, while only a small fraction (0.03% of T4 and 0.3% of T3) remains unbound or free, which is the biologically active form that can enter cells and tissues to exert its physiological effects.
There are three main types of thyroxine-binding proteins:
1. Thyroxine-binding globulin (TBG): This is the major thyroid hormone transport protein, synthesized in the liver and accounting for approximately 70-80% of T4 and T3 binding. TBG has a high affinity but low capacity for thyroid hormones.
2. Transthyretin (TTR), also known as prealbumin: This protein accounts for around 10-20% of T4 and T3 binding. It has a lower affinity but higher capacity for thyroid hormones compared to TBG.
3. Albumin: This is the most abundant protein in the blood and binds approximately 15-20% of T4 and a smaller fraction of T3. Although albumin has a low affinity for thyroid hormones, its high concentration allows it to contribute significantly to their transport.
The binding of thyroid hormones to these proteins helps maintain stable levels in the blood and ensures a steady supply to tissues. Additionally, TBPs protect thyroid hormones from degradation and rapid clearance by the kidneys, thereby extending their half-life in the circulation.
Serum globulins are a group of proteins present in the liquid portion of blood, known as serum. They are produced by the immune system in response to foreign substances such as bacteria, viruses, and allergens. Serum globulins include several types of immunoglobulins (antibodies), complement components, and other proteins involved in the immune response.
The serum globulin level is often measured as part of a complete blood count (CBC) or a protein electrophoresis test. An elevated serum globulin level may indicate an ongoing infection, inflammation, or an autoimmune disorder. Conversely, a decreased level may suggest a liver or kidney disease, or a malnutrition condition. It is important to note that the interpretation of serum globulin levels should be done in conjunction with other laboratory and clinical findings.
Blood protein electrophoresis (BPE) is a laboratory test that separates and measures the different proteins in the blood, such as albumin, alpha-1 globulins, alpha-2 globulins, beta globulins, and gamma globulins. This test is often used to help diagnose or monitor conditions related to abnormal protein levels, such as multiple myeloma, macroglobulinemia, and other plasma cell disorders.
In this test, a sample of the patient's blood is placed on a special gel and an electric current is applied. The proteins in the blood migrate through the gel based on their electrical charge and size, creating bands that can be visualized and measured. By comparing the band patterns to reference ranges, doctors can identify any abnormal protein levels or ratios, which may indicate underlying medical conditions.
It's important to note that while BPE is a useful diagnostic tool, it should be interpreted in conjunction with other clinical findings and laboratory tests for accurate diagnosis and management of the patient's condition.
Thyroxine-binding globulin (TBG) is a glycoprotein found in human plasma that has a high affinity for binding thyroid hormones, specifically Thyroxine (T4) and Triiodothyronine (T3). It is produced by the liver and plays a crucial role in maintaining the balance of these hormones in the body. TBG binds to approximately 70-80% of circulating T4 and about 55% of circulating T3, acting as a transport protein that carries these hormones throughout the body. The amount of TBG in the blood can vary due to factors such as genetics, sex hormones, and certain medications, which can affect the levels of free (unbound) thyroid hormones and contribute to various thyroid-related disorders.
Click chemistry is a term used to describe a group of chemical reactions that are fast, high-yielding, and highly selective. These reactions typically involve the formation of covalent bonds between two molecules in a simple and efficient manner, often through the use of a catalyst. The concept of click chemistry was first introduced by K. B. Sharpless, who won the Nobel Prize in Chemistry in 2001 for his work on chiral catalysis.
In the context of medical research and drug development, click chemistry has emerged as a valuable tool for rapidly synthesizing and optimizing small molecule compounds with therapeutic potential. By using click chemistry reactions to quickly and efficiently link different chemical building blocks together, researchers can rapidly generate large libraries of potential drug candidates and then screen them for biological activity. This approach has been used to discover new drugs for a variety of diseases, including cancer, infectious diseases, and neurological disorders.
One common type of click chemistry reaction is the copper-catalyzed azide-alkyne cycloaddition (CuAAC) reaction, which involves the reaction between an azide and an alkyne to form a triazole ring. This reaction is highly selective and can be carried out under mild conditions, making it a popular choice for chemical synthesis in the life sciences. Other types of click chemistry reactions include the Diels-Alder cycloaddition, the thiol-ene reaction, and the Staudinger ligation.
Overall, click chemistry has had a significant impact on medical research and drug development by enabling the rapid and efficient synthesis of complex small molecule compounds with therapeutic potential. Its versatility and selectivity make it a powerful tool for researchers seeking to discover new drugs and better understand the molecular mechanisms underlying human disease.
Genetic variation refers to the differences in DNA sequences among individuals and populations. These variations can result from mutations, genetic recombination, or gene flow between populations. Genetic variation is essential for evolution by providing the raw material upon which natural selection acts. It can occur within a single gene, between different genes, or at larger scales, such as differences in the number of chromosomes or entire sets of chromosomes. The study of genetic variation is crucial in understanding the genetic basis of diseases and traits, as well as the evolutionary history and relationships among species.
Thyroxine (T4) is a type of hormone produced and released by the thyroid gland, a small butterfly-shaped endocrine gland located in the front of your neck. It is one of two major hormones produced by the thyroid gland, with the other being triiodothyronine (T3).
Thyroxine plays a crucial role in regulating various metabolic processes in the body, including growth, development, and energy expenditure. Specifically, T4 helps to control the rate at which your body burns calories for energy, regulates protein, fat, and carbohydrate metabolism, and influences the body's sensitivity to other hormones.
T4 is produced by combining iodine and tyrosine, an amino acid found in many foods. Once produced, T4 circulates in the bloodstream and gets converted into its active form, T3, in various tissues throughout the body. Thyroxine has a longer half-life than T3, which means it remains active in the body for a more extended period.
Abnormal levels of thyroxine can lead to various medical conditions, such as hypothyroidism (underactive thyroid) or hyperthyroidism (overactive thyroid). These conditions can cause a range of symptoms, including weight gain or loss, fatigue, mood changes, and changes in heart rate and blood pressure.
Isoelectric focusing
Pier Giorgio Righetti
QPNC-PAGE
Acid dissociation constant
Isoelectric point
Eastern blot
Immobilized pH gradient
BBE-like enzymes
Protein mass spectrometry
Protein
Shotgun proteomics
Core-shell semiconductor nanocrystal
Zingibain
Droplet-based microfluidics
ACES (buffer)
Two-dimensional gel electrophoresis
Tubulin
Staphylococcus aureus beta toxin
Alpha-1 antitrypsin
Amy Herr
Metaproteomics
Amyloidosis
Amphoterism
Tetrasodium tris(bathophenanthroline disulfonate)ruthenium(II)
Bio-MEMS
ESD (gene)
SDS-PAGE
Siamese fighting fish
Gliadin
Luschan's salamander
Isoelectric focusing - Wikipedia
Carbonic Anhydrase I Isoelectric focusing marker, pI 6.6 9001-03-0
Identification of |i|Oreochromis niloticus|/i| (Linnaeus, 1758) and |i|Tilapia zilli|/i| (Gervais, 1848) using Isoelectric...
IsoGel® Isoelectric-Focusing Agarose, Lonza | VWR
Isoelectric Focusing System Components
Isoelectric focusing studies of human serum and tissue isoamylases - Fingerprint - Experts@Minnesota
Splicing of platelet resident pre-mRNAs upon activation by physiological stimuli results in functionally relevant proteome...
isoelectric focusing QuickView - Correlation Engine
Native Capillary Isoelectric Focusing for the Separation of Protein Complex Isoforms and Subcomplexes
System and method for detecting components of a mixture including a valving scheme for competition assays (Patent) | DOE Patents
Download Techniques Of Sample Preraration For Liquid Scintillation Counting Isoelectric Focusing
Analysis of aqueous humor immunoglobulin G in uveitis by enzyme-linked immunosorbent assay, isoelectric focusing, and...
Preparative denaturing isoelectric focusing for enhancing sensitivity of proteomic studies // Bond Life Sciences Center
Emergence of Ceftriaxone-Resistant Salmonella Isolates and Rapid Spread of Plasmid-Encoded CMY-2-Like Cephalosporinase, Taiwan ...
Optimized two-dimensional gel electrophoresis in an alkaline pH range improves the identification of intracellular CFDA...
The prospect of direct coupling of immunoaffinity capture and capillary isoelectric focusing in analysis of protein drugs |...
Separation and direct detection of raw and gelatinized starch hydrolyzing activities of glucoamylase on isoelectric focusing...
Coupling of Solid-Phase Microextraction and Capillary Isoelectric Focusing with Laser-Induced Fluorescence Whole Column Imaging...
Characterization of genetic variants of human serum transferrin by isoelectric focusing: Comparison between conventional and...
Membrane proteome analysis of microdissected ovarian tumor tissues using capillary isoelectric focusing/reversed-phase liquid...
Membrane proteome analysis of microdissected ovarian tumor tissues using capillary isoelectric focusing/reversed-phase liquid...
Analysis of genetic variation in two human thyroxine-binding plasma proteins by immunodetection after isoelectric focusing -...
Cerebrospinal fluid oligoclonal banding & IgG/Albumin - Immunology Laboratory
Biotechnology | Esslingen University
IJNS | Free Full-Text | Newborn Screening for Sickle Cell Disease and Other Hemoglobinopathies: A Short Review on Classical...
5) Electrophoresis.pptx
Global Hemoglobinopathies Market Share to hit at a CAGR of 10.1% by 2027 - PharmiWeb.com
PRIME PubMed | Characterization of genomes and chromosomes in partial amphiploids of the hybrid Triticum aestivum x Thinopyrum...
Publications | Borkholder Lab | RIT
'Electrophoresis in Practice' von 'Reiner Westermeier' - 'Gebundene Ausgabe' - '978-3-527-31181...
Electrophoresis5
- Isoelectric focusing is the first step in two-dimensional gel electrophoresis, in which proteins are first separated by their pI value and then further separated by molecular weight through SDS-PAGE. (wikipedia.org)
- Isoelectric focusing is the only step in preparative one-dimensional gel electrophoresis. (wikipedia.org)
- Carbonic Anhydrase I from human erythrocytes has been used as a pI (isoelectric point) marker in two-dimensional gel electrophoresis. (sigmaaldrich.com)
- Both lipids and glycogen are known to interfere with the first step in the two-dimensional polyacrylamide gel electrophoresis (2D-PAGE), the isoelectric focusing. (hindawi.com)
- Electrophoresis in which a pH gradient is established in a gel medium and proteins migrate until they reach the site (or focus) at which the pH is equal to their isoelectric point. (bvsalud.org)
Form a unique fingerprint1
- Dive into the research topics of 'Membrane proteome analysis of microdissected ovarian tumor tissues using capillary isoelectric focusing/reversed-phase liquid chromatography-tandem MS'. Together they form a unique fingerprint. (elsevierpure.com)
Separation6
- 2DE analysis of alkaline proteins is challenging due to the difficult separation of basic proteins during the isoelectric focusing (IEF). (nih.gov)
- My colleagues and I recently developed a method to couple immunoaffinity capture and separation by isoelectric focusing in a single capillary. (nii.ac.jp)
- The method allows sample pretreatment, i.e., purification and concentration, in a single capillary, in which subsequent separation by isoelectric focusing is achieved. (nii.ac.jp)
- Evaluation of protein loading techniques and improved separation in OFFGEL isoelectric focusing. (mpg.de)
- Salts, ionic molecules and ionic detergents interfere with isoelectric focusing and compromise protein separation. (thermofisher.com)
- Inactivation of the catalytic activity with organophosphate pesticides did not change the isoelectric point of the isozymes, and therefore separation of native from organophosphate inhibited AChE did not occur. (cdc.gov)
Preparative1
- 3.7 Preparative isoelectric focusing. (osiander.de)
Serum transferrin1
- Isoelectric focusing (IEF) of serum transferrin (Tf) is still the method of choice for diagnosis of congenital disorders of glycosylation (CDG). (nih.gov)
Proteins5
- Isoelectric focusing can resolve proteins that differ in pI value by as little as 0.01. (wikipedia.org)
- According to some opinions, living eukaryotic cells perform isoelectric focusing of proteins in their interior to overcome a limitation of the rate of metabolic reaction by diffusion of enzymes and their reactants, and to regulate the rate of particular biochemical processes. (wikipedia.org)
- The detergent-based membrane protein preparation protocol not only extracts proteins effectively from cell pellets but also is compatible with subsequent proteome analysis using combined capillary isoelctric focusing/nano reversed-phase liquid chromatography separations coupled with nano electrospray ionization mass spectrometry. (arizona.edu)
- In the first-dimension, proteins are separated according to their isoelectric point (pI)-the pH at which the protein net charge is zero-in a process known as isoelectric focusing (IEF). (thermofisher.com)
- During conventional IEF, amphoteric molecules, like proteins, migrate until they reach a region where the pH of the matrix matches their isoelectric point and they "focus" into sharp bands. (thermofisher.com)
Capillary1
- Capillary isoelectric focusing efficiently analyze heterogeneity of protein drugs. (nii.ac.jp)
Oligoclonal2
- Isoelectric focusing and immunoblotting studies revealed oligoclonal IgG bands in the aqueous of 13 of 23 (57%) patients with FHC, most being of the IgG1 subclass. (birmingham.ac.uk)
- 3. Positive findings in a cerebrospinal fluid (CSF) specimen (isoelectric focusing evidence of oligoclonal bands and/or elevated immunoglobulin (Ig) G index) [If a recent CSF sample or results from a previous CSF test from each patient is not available, this data will be considered missing information]. (who.int)
Immobilized pH gradients3
- The polymorphism of transferrin (Tf) is currently being studied by isoelectric focusing in carrier ampholyte-generated pH gradients, carrier ampholyte-separator pH gradients or in immobilized pH gradients. (unicatt.it)
- Tf subtype resolution was only achieved by using isoelectric focusing in immobilized pH gradients with pH slopes reliably reproducible from one experiment to another. (unicatt.it)
- A system using immobilized pH gradients for isoelectric focusing (IEF) was also developed providing greater reproducibility of 2-dimensional patterns. (europa.eu)
Gels1
- A procedure to detect raw and gelatinized starch activities of glucoamylase on isoelectric focusing (IEF) gels by using 2, 3, 5-triphenyltetrazolium chloride is described. (uni-mysore.ac.in)
Methodology2
Gradients1
- Isoelectric focusing in carrier ampholyte-separator pH gradients cannot be recommended as a standard typing procedure because the results strongly depend on the batch of carrier ampholytes. (unicatt.it)
Molecules3
- Isoelectric focusing (IEF), also known as electrofocusing, is a technique for separating different molecules by differences in their isoelectric point (pI). (wikipedia.org)
- As a particle moves toward the pole opposite of its charge it moves through the changing pH gradient until it reaches a point in which the pH of that molecules isoelectric point is reached. (wikipedia.org)
- By modification of the isoelectric point (pI) of molecules of an enzyme by, e.g., phosphorylation or dephosphorylation, the cell can transfer molecules of the enzyme between different parts of its interior, to switch on or switch off particular biochemical processes. (wikipedia.org)
Experiments1
- Carbonic Anhydrase I from human erythrocytes is a protein which is used as a standard pI (isoelectric point) marker for isoelectric focussing experiments. (sigmaaldrich.com)
Point2
- A protein that is in a pH region below its isoelectric point (pI) will be positively charged and so will migrate toward the cathode (negatively charged electrode). (wikipedia.org)
- Small changes in the protein mass or isoelectric point (pI) translate into a detectable protein shift. (thermofisher.com)
Method1
- The method consisted of isoelectric focusing (IEF) of dog serum, followed by immunoblotting. (lu.se)
Handbook Of Isoelectric Focusing And Proteomics2
- Otto not are an British download handbook of isoelectric focusing and proteomics 2005 of what Description jeweils somatically Then. (erzebet.com.ar)
- The Regent Taktse Shabdrung and little songs n't was a download handbook of isoelectric focusing and proteomics 2005 to the clinical political pp. that they was Kelsang Gyatso as the Dalai Lama. (erzebet.com.ar)
HPLC2
- Isoelectric focusing nonporous RP HPLC: a two-dimensional liquid-phase separation method for mapping of cellular proteins with identification using MALDI-TOF mass spectrometry. (nih.gov)
- The proteins are separated by pI using isoelectric focusing in the first dimension and by hydrophobicity using nonporous reversed-phase HPLC in the second dimension (IEF-NP RP HPLC). (nih.gov)
Proteomics1
- A proteomics platform combining depletion, multi-lectin affinity chromatography (M-LAC), and isoelectric focusing to study the breast cancer proteome. (nih.gov)
Protein3
- A protein that is in a pH region below its isoelectric point (pI) will be positively charged and so will migrate toward the cathode (negatively charged electrode). (wikipedia.org)
- A universal indicator dye demonstrates the generation of stable, reversible pH gradients (3-10) in ampholyte buffers, and these gradients lead to protein focusing within the drop length. (elsevierpure.com)
- The reduced protein had a pI of 5.1 as judged by isoelectric focusing. (nih.gov)
Polyacrylamide1
- All three preparations were fractionated by membrane or Sephadex ultrafiltration and iso-electric focusing in polyacrylamide gels, as well as being examined by immunoelectrophoretic techniques. (gla.ac.uk)
Agarose gels2
- and in isoelectric focusing to minimize endoosmotic flow in agarose gels. (sigmaaldrich.com)
- There was a complete agreement that isoelectric focusing on agarose gels followed by immunoblotting should be the "gold standard" for detecting the presence of oligoclonal bands. (sebia.com)
Separation2
- Since beta-lactamases may be universally produced by bacteria, separation of the enzymes by analytical isoelectric focusing could be used in bacterial taxonomy. (nih.gov)
- The collected glycoproteomes were then subjected to isoelectric focusing (IEF) separation by a digital ProteomeChip (dPC), followed by in-gel digestion and LC-MS analysis using an Orbitrap mass spectrometer. (nih.gov)
Recombinant1
- Detection of isoforms of recombinant human erythropoietin by various plant lectins after isoelectric focusing. (tuwien.at)
Detection1
- The use of analytical isoelectric focusing for detection and identification of beta-lactamases. (nih.gov)
Migration2
- They were both acidic based on their migration on an acrylamide isoelectric focusing (IEF) gel. (usda.gov)
- With arginines at both positions, ApoE4 is the most basic of the three species and was designated 4 based on its migration upon isoelectric focusing (see Nomenclature Notes below). (alzforum.org)
Purification1
- Chromatofocusing purification of CD1b-antigen complexes and their analysis by isoelectric focusing. (illumina.com)
Analysis2
- Currently his research is focusing on the elimination of organic solvents from the sample preparation step to facilitate on-site monitoring and in-vivo analysis. (uwaterloo.ca)
- Calf lens homogenates were incubated at temperatures ranging from 37 to 45 degrees-C. Isoelectric focused gel analysis of the lenses incubated for 30 minutes at 45 degrees showed a definite loss in the lowest points of the alpha crystallin components. (cdc.gov)
Molecular2
- A novel approach to molecular separations is investigated using a technique termed droplet-based isoelectric focusing. (elsevierpure.com)
- The loss of alpha crystallin from the isoelectric focusing pattern was due to aggregation to higher molecular weight particles which could not enter the gel. (cdc.gov)
Results1
- We review the results of these comparisons, focusing on yeast. (biomedcentral.com)