The systematic study of the complete complement of proteins (PROTEOME) of organisms.
Narrow pieces of material impregnated or covered with a substance used to produce a chemical reaction. The strips are used in detecting, measuring, producing, etc., other substances. (From Dorland, 28th ed)
The protein complement of an organism coded for by its genome.
Use of a metal casting, usually with a post in the pulp or root canal, designed to support and retain an artificial crown.
An analytical method used in determining the identity of a chemical based on its mass using mass analyzers/mass spectrometers.
A mass spectrometry technique using two (MS/MS) or more mass analyzers. With two in tandem, the precursor ions are mass-selected by a first mass analyzer, and focused into a collision region where they are then fragmented into product ions which are then characterized by a second mass analyzer. A variety of techniques are used to separate the compounds, ionize them, and introduce them to the first mass analyzer. For example, for in GC-MS/MS, GAS CHROMATOGRAPHY-MASS SPECTROMETRY is involved in separating relatively small compounds by GAS CHROMATOGRAPHY prior to injecting them into an ionization chamber for the mass selection.
Electrophoresis in which a second perpendicular electrophoretic transport is performed on the separate components resulting from the first electrophoresis. This technique is usually performed on polyacrylamide gels.
Water swollen, rigid, 3-dimensional network of cross-linked, hydrophilic macromolecules, 20-95% water. They are used in paints, printing inks, foodstuffs, pharmaceuticals, and cosmetics. (Grant & Hackh's Chemical Dictionary, 5th ed)
Societies whose membership is limited to physicians.
The minute vessels that connect the arterioles and venules.
Sequential operating programs and data which instruct the functioning of a digital computer.
Directions or principles presenting current or future rules of policy for assisting health care practitioners in patient care decisions regarding diagnosis, therapy, or related clinical circumstances. The guidelines may be developed by government agencies at any level, institutions, professional societies, governing boards, or by the convening of expert panels. The guidelines form a basis for the evaluation of all aspects of health care and delivery.
Silicone polymers which consist of silicon atoms substituted with methyl groups and linked by oxygen atoms. They comprise a series of biocompatible materials used as liquids, gels or solids; as film for artificial membranes, gels for implants, and liquids for drug vehicles; and as antifoaming agents.
Occasions to commemorate an event or occasions designated for a specific purpose.
Time period from 1901 through 2000 of the common era.
Apparatus, devices, or supplies intended for one-time or temporary use.
Time period from 2001 through 2100 of the common era.
Such mixtures of amphoteric electrolytes or buffers that provide a continuous range of pH in an electric field; used for separating proteins by their isoelectric points, i.e., by isoelectric focusing.
Management control systems for structuring health care delivery strategies around case types, as in DRGs, or specific clinical services.
Electrophoresis in which a pH gradient is established in a gel medium and proteins migrate until they reach the site (or focus) at which the pH is equal to their isoelectric point.
The pH in solutions of proteins and related compounds at which the dipolar ions are at a maximum.
Electrophoresis in which a polyacrylamide gel is used as the diffusion medium.
The sum of the weight of all the atoms in a molecule.
A contagious disease caused by canine adenovirus (ADENOVIRUSES, CANINE) infecting the LIVER, the EYE, the KIDNEY, and other organs in dogs, other canids, and bears. Symptoms include FEVER; EDEMA; VOMITING; and DIARRHEA.
Diseases of the domestic dog (Canis familiaris). This term does not include diseases of wild dogs, WOLVES; FOXES; and other Canidae for which the heading CARNIVORA is used.
Diseases of plants.
A compound obtained from the bark of the white willow and wintergreen leaves. It has bacteriostatic, fungicidal, and keratolytic actions.
Proteins found in plants (flowers, herbs, shrubs, trees, etc.). The concept does not include proteins found in vegetables for which VEGETABLE PROTEINS is available.
The oxygen-carrying proteins of ERYTHROCYTES. They are found in all vertebrates and some invertebrates. The number of globin subunits in the hemoglobin quaternary structure differs between species. Structures range from monomeric to a variety of multimeric arrangements.
Hemoglobins characterized by structural alterations within the molecule. The alteration can be either absence, addition or substitution of one or more amino acids in the globin part of the molecule at selected positions in the polypeptide chains.
A compound formed by the combination of hemoglobin and oxygen. It is a complex in which the oxygen is bound directly to the iron without causing a change from the ferrous to the ferric state.
Normal adult human hemoglobin. The globin moiety consists of two alpha and two beta chains.
An element with atomic symbol O, atomic number 8, and atomic weight [15.99903; 15.99977]. It is the most abundant element on earth and essential for respiration.
The largest order of CRUSTACEA, comprising over 10,000 species. They are characterized by three pairs of thoracic appendages modified as maxillipeds, and five pairs of thoracic legs. The order includes the familiar shrimps, crayfish (ASTACOIDEA), true crabs (BRACHYURA), and lobsters (NEPHROPIDAE and PALINURIDAE), among others.
A compound formed in the liver from ammonia produced by the deamination of amino acids. It is the principal end product of protein catabolism and constitutes about one half of the total urinary solids.
A family of CRUSTACEA, order DECAPODA, comprising the penaeid shrimp. Species of the genus Penaeus are the most important commercial shrimp throughout the world.
Techniques for standardizing and expediting taxonomic identification or classification of organisms that are based on deciphering the sequence of one or a few regions of DNA known as the "DNA barcode".
Colloids with a solid continuous phase and liquid as the dispersed phase; gels may be unstable when, due to temperature or other cause, the solid phase liquefies; the resulting colloid is called a sol.

Differential expression and phosphorylation of CTCF, a c-myc transcriptional regulator, during differentiation of human myeloid cells. (1/3619)

CTCF is a transcriptional repressor of the c-myc gene. Although CTCF has been characterized in some detail, there is very little information about the regulation of CTCF activity. Therefore we investigated CTCF expression and phosphorylation during induced differentiation of human myeloid leukemia cells. We found that: (i) both CTCF mRNA and protein are down-regulated during terminal differentiation in most cell lines tested; (ii) CTCF down-regulation is retarded and less pronounced than that of c-myc; (iii) CTCF protein is differentially phosphorylated and the phosphorylation profiles depend on the differentiation pathway. We concluded that CTCF expression and activity is controlled at transcriptional and post-transcriptional levels.  (+info)

Human triclonal anti-IgG gammopathy. I. Iso-electric focusing characteristics of the IgG, IgA and IgM anti-IgG and their heavy and light chains. (2/3619)

Human IgG, IgA and IgM anti-IgG autoantibodies have been isolated from the serum of an individual with Felty's syndrome. These were initially noted as soluble circulating serum complexes by analytical ultracentrifugation. Isolation was accomplished by solid phase immunoadsorption and each of the three antibody populations obtained was shown to be of restricted heterogeneity by liquid and polyacrylamide gel electrofocussing methods. Type kappa light chains were obtained from each protein. Co-isoelectric focusing experiments of all possible pairs of these light chains showed them to have identical net charge characteristics. Heavy chains obtained from each protein were also monoclonal and of differing isoelectric point. The availability of this serum provides a human model with which to study the changes which may occur in autoantibodies during the autoimmune response.  (+info)

Molecular and biochemical characterization of VEB-1, a novel class A extended-spectrum beta-lactamase encoded by an Escherichia coli integron gene. (3/3619)

A clinical isolate, Escherichia coli MG-1, isolated from a 4-month-old Vietnamese orphan child, produced a beta-lactamase conferring resistance to extended-spectrum cephalosporins and aztreonam. In a disk diffusion test, a typical synergistic effect between ceftazidime or aztreonam and clavulanic acid was observed along with an unusual synergy between cefoxitin and cefuroxime. The gene for VEB-1 (Vietnamese extended-spectrum beta-lactamase) was cloned and expressed in E. coli JM109. The recombinant plasmid pRLT1 produced a beta-lactamase with a pI of 5.35 and conferred high-level resistance to extended-spectrum (or oxyimino) cephalosporins and to aztreonam. Vmax values for extended-spectrum cephalosporins were uncommonly high, while the affinity of the enzyme for ceftazidime and aztreonam was relatively low. blaVEB-1 showed significant homology at the DNA level with only blaPER-1 and blaPER-2. Analysis of the deduced protein sequence showed that VEB-1 is a class A penicillinase having very low levels of homology with any other known beta-lactamases. The highest percentage of amino acid identity was 38% with PER-1 or PER-2, two uncommon class A extended-spectrum enzymes. Exploration of the genetic environment of blaVEB-1 revealed the presence of gene cassette features, i.e., (i) a 59-base element associated with blaVEB-1; (ii) a second 59-base element just upstream of blaVEB-1, likely belonging to the aacA1-orfG gene cassette; (iii) two core sites (GTTRRRY) on both sides of blaVEB-1; and (iv) a second antibiotic resistance gene 3' of blaVEB-1, aadB. blaVEB-1 may therefore be the first class A extended-spectrum beta-lactamase that is part of a gene cassette, which itself is likely to be located on a class 1 integron, as sulfamide resistance may indicate. Furthermore, blaVEB-1 is encoded on a large (> 100-kb) transferable plasmid found in a Klebsiella pneumoniae MG-2 isolated at the same time from the same patient, indicating a horizontal gene transfer.  (+info)

Mechanisms of beta-lactam resistance amongst Pseudomonas aeruginosa isolated in an Italian survey. (4/3619)

The mechanisms of resistance to beta-lactam antibiotics in 325 isolates of Pseudomonas aeruginosa were examined. These isolates were selected because of their resistance to meropenem and imipenem (breakpoint, >4 mg/L), carbenicillin (>128 mg/L), ceftazidime (>8 mg/L), piperacillin and ticarcillin/clavulanate (>64 mg/L). The most frequent mechanism of resistance was beta-lactamase-independent, so called 'intrinsic resistance', which was found in 183 isolates and was probably due to impermeability and/or efflux mechanisms. beta-Lactamase-mediated resistance was demonstrated in 111 strains (11.1%). Derepression of Ambler Class C chromosomal beta-lactamase was detected in 64 isolates, most of which were resistant to ceftazidime and piperacillin but susceptible to meropenem, whereas secondary plasmid-encoded beta-lactamases were found in 34 isolates, all of them resistant to carboxypenicillins and ureidopenicillins and susceptible to carbapenems. Twelve strains showed more than one plasmid-encoded beta-lactamase plus derepression of chromosomal Class C enzyme. Resistance to carbapenems was independent of resistance to other beta-lactam antibiotics, indicating a different mechanism of resistance, probably due to the loss of the D2 porin. In total, 32 strains were resistant to carbapenems: 24 only to imipenem and eight to both imipenem and meropenem.  (+info)

Differential serodiagnosis for cystic and alveolar echinococcosis using fractions of Echinococcus granulosus cyst fluid (antigen B) and E. multilocularis protoscolex (EM18). (5/3619)

Echinococcus granulosus cyst fluid and E. multilocularis protoscolex extract were fractionated by a single step of preparative isoelectric focusing, resulting in an antigen B-rich fraction (8-kD) and an Em18-rich fraction, respectively. The usefulness of both fractions for differential serodiagnosis of cystic (CE) and alveolar (AE) echinococcosis was evaluated by a large-scale immunoblot analysis on a battery of 354 serum samples. These included 66 from AE patients originating from four different endemic areas, 173 from CE patients originating from seven different endemic areas, 71 from patients with other parasitic diseases, 15 from patients with hepatomas, and 29 from healthy individuals. In an immunoblot with the antigen B-rich fraction, 92% (158 of 173) of the CE sera as well as 79% (52 of 66) of the AE sera reacted with the 8-kD subunit. No cross-reactivity occurred with any sera from patients with cysticercosis, other parasitic diseases, or with hepatomas, or from healthy controls. In an immunoblot with the Em18-rich fraction, all but two sera from AE patients (64 of 66, 97%) recognized Em18, and only nine of 34 CE sera from China reacted with it. All other (139) CE sera from six other countries were negative as were all (115) other non-echinococcosis sera. These findings indicate that antigen B (8-kD) is not species-specific for E. granulosus but is genus-specific for Echinococcus, and that the Em18 antigen is a reliable serologic marker for species-specific differentiation of AE from CE.  (+info)

The relationship of glycosylation and isoelectric point with tumor accumulation of avidin. (6/3619)

Radiolabeled avidin markedly accumulated in intraperitoneal tumors and was cleared rapidly from circulation when given intraperitoneally. This study investigated the mechanisms of the tumor localization of avidin. METHODS: Avidin was deglycosylated through endoglycosydase-H digestion and/or neutralized by acetylation of its lysine amino acids with acetic acid N-hydroxysuccinimide ester. Avidin and modified avidins were analyzed using sodium dodecylsulfate-polyacrylamide gel electrophoresis (SDS/PAGE) and isoelectric focusing. A tumor model was established by intraperitoneal injection of human colon cancer cells, LS180, in nude mice. Avidin and modified avidins were labeled with 111In using diethyleneamine pentaacetic acid-biotin and were administered intraperitoneally into the tumor-bearing mice. The biodistribution of radioactivity was examined 2 and 24 h postinjection. RESULTS: Deglycosylated avidins revealed a major band of smaller molecules on SDS/PAGE. The isoelectric point of neutralized avidins was reduced to less than 5, whereas that of unneutralized avidins was more than 9.5. Biodistribution study demonstrated that liver uptake was decreased by deglycosylation and kidney accumulation was decreased by neutralization, respectively. The blood clearance was remarkably slowed by combined modification of deglycosylation and neutralization. The tumor uptake of radioactivity was reduced by either deglycosylation or neutralization and was further decreased with combined modification. CONCLUSION: Both high glycosylation and positive charge of avidin contributed to its accumulation in tumor. This study may facilitate development of a new vehicle for the delivery of therapeutic agents to intraperitoneal tumors.  (+info)

Isolation of pigment-binding early light-inducible proteins from pea. (7/3619)

The early light-inducible proteins (ELIPs) in chloroplasts possess a high sequence homology with the chlorophyll a/b-binding proteins but differ from those proteins by their substoichiometric and transient appearance. In the present study ELIPs of pea were isolated by a two-step purification strategy: perfusion chromatography in combination with preparative isoelectric focussing. Two heterogeneous populations of ELIPs were obtained after chromatographic separation of solubilized thylakoid membranes using a weak anion exchange column. One of these populations contained ELIPs in a free form providing the first isolation of these proteins. To prove whether the isolated and pure forms of ELIP bind pigments, spectroscopic and chromatographic analysis were performed. Absorption spectra and TLC revealed the presence of chlorophyll a and lutein. Measurements of steady-state fluorescence emission spectra at 77 K exhibited a major peak at 674 nm typical for chlorophyll a bound to the protein matrix. The action spectrum of the fluorescence emission measured at 674 nm showed several peaks originating mainly from chlorophyll a. It is proposed that ELIPs are transient chlorophyll-binding proteins not involved in light-harvesting but functioning as scavengers for chlorophyll molecules during turnover of pigment-binding proteins.  (+info)

Low-temperature sensitivity and enhanced Bohr effect in red deer (Cervus elaphus) haemoglobin: a molecular adaptive strategy to life at high altitude and low temperature. (8/3619)

A study of the functional properties of haemoglobin from red deer (Cervus elaphus) whose habitat varies over a wide range of latitude, was performed. The oxygen-binding properties of the most common haemoglobin phenotype from the species living in Sardinia were examined with particular attention to the effect of pH, chloride, 2, 3-bisphosphoglycerate and temperature. Results indicate that red deer haemoglobin, like all haemoglobins from ruminants so far examined, is characterized by a low intrinsic oxygen affinity, with chloride being its main physiological modulator in vivo. The functional results and the low temperature sensitivity of the oxygen affinity are discussed in the light of the amino acid sequence of closely related ruminant haemoglobins.  (+info)

The nuclear proteins of regenerating and fetal rat liver, slow-growing Morris hepatoma 9618A, and fast-growing Morris hepatoma 3924A, sequentially extracted from nuclei with (a) 0.075 m NaCl/0.025 m EDTA, (b) 10 mm Tris, (c) 0.35 m NaCl, (d) 0.6 m NaCl, and (e) 3 m NaCl/7 m urea, were analyzed by two-dimensional, isoelectric-focusing sodium dodecyl sulfate gel electrophoresis. Many of the protein spots were common to these tissues as well as to the Novikoff hepatoma and normal liver. The protein patterns of the regenerating liver and slow-growing 9618A hepatoma were more similar to that of normal liver than to those of other tissues. Many similarities were found between the fetal liver and the fast-growing 3924A and Novikoff hepatomas. Four protein spots, 64/5.9, 60/6.3, 51/5.3, and 38/7.3 (M.W. × 10-3/pl), were only found in the fast-growing 3924A and Novikoff hepatomas. Proteins 79/6.4 and 61/7.2 were only found in the three hepatomas. Protein 37/6.3 was dense in the three hepatomas and much ...
R. L. Prestidge, W. J. Koopman, J. C. Bennett, M. T. W. Hearn; Buffer electrofocusing of Interleukin I. Biosci Rep 1 April 1982; 2 (4): 241-246. doi: https://doi.org/10.1007/BF01136723. Download citation file:. ...
Charge heterogeneity of monoclonal antibodies is an important critical quality attribute that requires close monitoring due to its potential impact on antibody efficacy and immunogenicity. Since the heterogeneity is mostly caused by post translational modifications such as C-terminal lysine clipping, deamidation, glycation, sialic acid or adduct formation, these modifications can pose significant challenges to the analytical scientists.. In a recently published article by Jaag et al., scientists from the Institute of Pharmaceutical Sciences at University at Tübingen, Germany, present a new approach to charge variant analysis at the intact and sub-unit level by 2D-LC separation. In this workflow, the first-dimension separation is based on strong-cation chromatography (SCX) and the second-dimension separation is based on desalting reversed phase liquid chromatography (RP-HPLC) which enables combination with the mass analysis by mass spectrometric (MS) detection.. The analysis at the subunit level ...
TY - JOUR. T1 - An investigation of the surface properties of atomized FexSi powders. AU - Williams, Richard Andrew. AU - Kelsall, Geoffrey Howard. PY - 1989/10/1. Y1 - 1989/10/1. N2 - The results of XPS and electrokinetic measurements on spherical ferrosilicide particles (15 wt% Si) produced by atomization show that the surface consists of a mixed silica and Fe(II, III) oxide layer of at least 50 nm thickness. Although the surface is dominated by the silica phase (up to 70 at %), the electrophoretic mobility response in aqueous KCl electrolytes is complex and exhibits three points of charge reversal over the pH range 3-11. These phenomena correspond to an apparent point of zero charge of the surface at about pH 5 (which varies with surface composition), a point of charge reversal (which is independent of surface composition) at pH 7 due to the adsorption of FeOH+ ions originating from the substrate, and an isoelectric point at pH 7.6 due to transformation of an essentially siliceous surface to ...
Plasminogen, the proenzyme of the fibrinolytically active enzyme plasmin, is a single chain glycoprotein with a molecular weight of about 90,000-94,000 Da (2). Various isoelectric forms exist and can be separated by means of isoelectric focusing (IEF).
A Poly-CCC (Poly Capillary Conic Collimator) is an X-ray poly capillary optics with a conically shaped basic form. Divergent X-rays are collimated and guided.
An inbred strain can produce several hundred different anti-group A carbohydrate (GAC) antibodies, as analyzed by isoelectric focusing. However, each individual mouse produces the bulk of its anti-GAC antibody as only one or two different spectrotypes, which appear to be randomly chosen. By using adoptive transfer techniques, we have observed that clonal commitment occurs very early in immunization, sometimes even before immunization, and thus does not result from competition among B cells for antigen ...
* found in: Pharmalyte, Pharmalyte, narrow range pH 4.2-4.9 Pharmalyte, narrow range pH 4.2-4.9 Pharmalyte carrier ampholytes, prepared by the co..
This summer, follow these six savvy rules of healthy eating: Eat Whole Foods, not processed Any food in its most essential, pure and basic form is nutrient dense and must …. ...
This summer, follow these six savvy rules of healthy eating: Eat Whole Foods, not processed Any food in its most essential, pure and basic form is nutrient dense and must …. ...
Two nanofiltration membranes, a Dow NF 270 polyamide thin film and a TriSep TS 80 polyamide thin film, were investigated for their retention of ionic species when filtering mine influenced water streams at a range of acidic pH values. The functional iso-electric point of the membranes, characterized by changes in retention over a small pH range, were examined by filtering solutions of sodium sulphate. Both membranes showed changes in retention at pH 3, suggesting a zero net charge on the membranes at this pH. Copper mine drainage and synthetic solutions of mine influenced water were filtered using the same membranes. These solutions were characterized by pH values within 2 and 5, thus crossing the iso-electric point of both membranes. Retention of cations was maximized when the feed solution pH was less than the iso-electric point of the membrane. In these conditions, the membrane has a net positive charge, reducing the transmission rate of cations. From the recoveries of a range of cations, the
Two nanofiltration membranes, a Dow NF 270 polyamide thin film and a TriSep TS 80 polyamide thin film, were investigated for their retention of ionic species when filtering mine influenced water streams at a range of acidic pH values. The functional iso-electric point of the membranes, characterized by changes in retention over a small pH range, were examined by filtering solutions of sodium sulphate. Both membranes showed changes in retention at pH 3, suggesting a zero net charge on the membranes at this pH. Copper mine drainage and synthetic solutions of mine influenced water were filtered using the same membranes. These solutions were characterized by pH values within 2 and 5, thus crossing the iso-electric point of both membranes. Retention of cations was maximized when the feed solution pH was less than the iso-electric point of the membrane. In these conditions, the membrane has a net positive charge, reducing the transmission rate of cations. From the recoveries of a range of cations, the ...
Brown, R.K., Caspers, M.L., Lull, J.M., Vinogradov, S.N., Felgenhauer, K. and Nekic, M. (1977). Carrier Ampholyte Distribution in Isoelectric Focusing. J. Chromatogr. 131, 223-232.. Brown, R.K., Caspers, M.L. and Vinogradov, S.N. (1977). Carrier Ampholyte Distribution. In Electrofocusing and Isotachophoresis (Radola, B.J. and Graesslin, D. Eds.) Walter DeGrutyer and Co., Berlin-New York, pp 87-96.. Caspers, M.L., Posey, Y. and Brown, R.K. (1977). Separator Isoelectric Focusing - An Improved Method of Protein Analysis and Purification. Anal. Biochem. 79, 166-180.. Caspers, M.L. and Chrambach, A. (1977). Natural pH Gradients Formed by Amino Acids: Ampholyte Distribution, Time Course, Use in Electrofocusing of Protein, Relation to pH Gradients in Isotachophoresis, Separator Effects. Anal. Biochem. 81, 23-39.. Caspers, M.L. and Siegel, G.J. (1980). Inhibition by Lead of Human Erythrocyte (Na+ + K+)-Adenosine Triphosphatase Associated with Binding of 210Pb to Membrane Fragments. Biochim. et. Biophy. ...
Isoelectric Focusing (IEF) can be described as an ingenious process for simultaneous concentration and separation of proteins. IEF employs a pH gradient formed by small amphoteric molecules (called ampholytes) to resolve proteins according to their different pI values (pI = isoelectric point). IEF is an end point method - when the electrophoretic run is completed proteins will appear as separate, sharp zones, in the order of their isoelectric points.. For IEF, SERVA offers a comprehensive product line: ...
We theoretically study the electrokinetic problem of a pressure-induced liquid flow through a narrow long channel with charged walls, going beyond the classical Helmholtz-Schmolukowski picture by considering the surprisingly strong combined effect of (i) Stern-layer conductance and (ii) dynamic chargeregulating rather than fixed surface charges. We find that the water flow induces, apart from the well-known streaming potential, also a strongly heterogeneous surface charge and zeta potential on chemically homogeneous channel walls. Moreover, we identify a novel steady state with a nontrivial 3D electric flux with 2D surface charges acting as sources and sinks. For a pulsed pressure drop our findings also provide a first-principles explanation for ill-understood experiments on the effect of flow on interfacial chemistry [D. Lis et al., Science 344, 1138 (2014 ...
Browse our electrophoresis products: carrier ampholytes, electrophoresis chamber, power supply, acrylamide gel support sheets and seed purity testing kits.
Two hundred twenty-two patients (168 women, 54 men) were included. The mean age was 52.2 ± 19.3 years (range 18-84 years). The mean follow-up was 28.8 ± 8.2 months (range 24-62 months). There was a significant correlation between PI − LL and PI (r = 0.441, p , 0.001), threatening the use of PI − LL to quantify spinopelvic mismatch for different PI values. RLL was not correlated with PI (r = −0.093, p , 0.05); therefore, it was able to quantify divergence from ideal lordosis for all PI values. Compared with PI − LL, RLL had stronger correlations with HRQOL scores (p , 0.05). Discrimination performance was better for the model with RLL than for PI − LL. The agreement between RLL and PI − LL was high (κ = 0.943, p , 0.001), moderate (κ = 0.455, p , 0.001), and poor (κ = −0.154, p = 0.343), respectively, for large, average, and small PI sizes. When analyzed by RLL, each PI − LL category was further divided into distinct groups of patients who had different mechanical ...
Two hundred twenty-two patients (168 women, 54 men) were included. The mean age was 52.2 ± 19.3 years (range 18-84 years). The mean follow-up was 28.8 ± 8.2 months (range 24-62 months). There was a significant correlation between PI − LL and PI (r = 0.441, p , 0.001), threatening the use of PI − LL to quantify spinopelvic mismatch for different PI values. RLL was not correlated with PI (r = −0.093, p , 0.05); therefore, it was able to quantify divergence from ideal lordosis for all PI values. Compared with PI − LL, RLL had stronger correlations with HRQOL scores (p , 0.05). Discrimination performance was better for the model with RLL than for PI − LL. The agreement between RLL and PI − LL was high (κ = 0.943, p , 0.001), moderate (κ = 0.455, p , 0.001), and poor (κ = −0.154, p = 0.343), respectively, for large, average, and small PI sizes. When analyzed by RLL, each PI − LL category was further divided into distinct groups of patients who had different mechanical ...
Results. At PUH 298 -patients -were oper-ated and 312 -patients at PI. -There -were 136 low -risk (EuroS-CORE 0-2) and 474 -medium and -high--risk -patients (EuroS-CORE ≥3). -There was no sig-nif-i-cantly ele-vated mor-tality or -learning -curve in low -risk sur-gery -either at PUH (57 -patients -with 1 -death) or PI (79 -patients 1 -death). In -medium and -high--risk sur-gery at PI -there was no mor-tality in 68 -patients oper-ated by the -senior sur-geon and no -learning -curve in all 233 -such -patients. In 240 -medium and -high--risk -patients at PUH -there was a -learning -curve -despite the involve-ment of the -same -senior sur-geon. In 1999 and 2000 the -observed mor-tality (OM) in 150 -patients was 15.33%, EuroS-CORE 5.98, and in 2001 in 91 -patients OM 3.29%, EuroS-CORE 5.95 -with p=0.0038 -when expe-ri-enced ICU -staff was -employed. LOS was sig-nif-i-cantly -reduced in 97 -patients in 2001 at PUH (8.7 d ± 2.81 vs 11.07-days ± 7.9 in 1999 and 2000, p=0.046) con-firming the ...
• Features an acidic form of betaine to aid digestion • Includes the digestive enzyme pepsin Betaine HCl and pepsin are naturally occurring gastric-juice components that render nutrients available for absorption and biological activity. Betaine HCl is an acidic form of betaine, which promotes optimal gastric lumen acid
native page separates based on charge (with size constraints due to limiting pore size of the gel). if you have two proteins of the same size but different pI then you should be able to resolve them on native or urea or ief page ...
Animal organs and organ systems constantly adjust to internal and external changes through a process called homeostasis (steady state). These changes might be in the level of glucose or calcium in blood or in external temperatures. Homeostasis means to maintain dynamic equilibrium in the body. It is dynamic because it is constantly adjusting to the changes that the bodys systems encounter. It is equilibrium because body functions are kept within specific ranges. Even an animal that is apparently inactive is maintaining this homeostatic equilibrium.. ...
AUBURN UNIVERSITY, Ala. - For farmers in Alabama, fall is one of the busiest times of the year. They are working around the clock to harvest and transport the crops they worked hard to grow. During this time, farmers use many different pi…. ...
UroToday - GU OncToday brings coverage of the clinically relevant content needed to stay at the forefront of the dynamic field of GU oncology and urology.
UroToday - GU OncToday brings coverage of the clinically relevant content needed to stay at the forefront of the dynamic field of GU oncology and urology.
compute the isoelectric point pi of each of the given amino acidsa glycineb glutamic acidc, Hire Biology Expert, Ask Academics Expert, Assignment Help, Homework Help, Textbooks Solutions
The PROTEAN i12 IEF System, from Bio-Rad Laboratories, is used for isoelectric focusing (IEF) of immobilized pH gradient (IPG) strips. The PROTEAN i12 IEF System can simultaneously run up to 12 IPG strips in 12 independently programmed lanes. Watch this video to learn about more unique features and benefits of this system, which can be used for in-gel peptide fractionation prior to LC-MS.
Buy plastic sheets as laser cut parts online at low prices. Numerous basic forms ✓ Various configuration possibilities ✓ polished laser edge ✓
Do animals have a basic form of telepathy? From incredible journeys of cats and dogs, to Jim the Wonder Dog, there are true stories that will make you wonder if the animal world has hidden secrets.
In its most basic form, factoring is very easy to understand. A company is growing and needs extra capital to make sure that bills are being paid on time. This includes operating bills or payroll and taxes. Factoring accounts receivable is where the finance company uses loans, in the form of invoices, to provide the outside capital companies need to keep growing.
Fucosyltransferase (FT) activity of normal lymphocytes, normal granulocytes, and various types of human leukemic cells and electrofocusing pattern of FT activity in human leukemic cells and normal lymphocytes were examined using asialofetuin as an acceptor. Levels of FT activity in normal lymphocytes were higher than those of normal granulocytes in which FT activity was almost undetectable. The FT activity was higher in blast cells of acute myeloblastic leukemia and chronic myelogenous leukemia in blast crisis than in blast cells of acute lymphoblastic leukemia and the chronic phase of chronic myelogenous leukemia. The level of FT activity was lower in cells of chronic lymphocytic leukemia than that of normal lymphocytes, but it was higher than that of normal granulocytes. Three main isoelectric forms of FT in leukemic blast cells were identified by isoelectrofocusing, and they each had a characteristic focusing point: around pH 4.5 (peak 1); pH 4.9 (peak 2); and pH 5.2 (peak 3). In blast cells ...
OBJECTIVES. Isoelectric focusing (IEF) is a laboratory technique that allows to separate proteic molecules based on their isoelectric point. This technique, applied to cerebro spinal fluid (CSF) IgG, has been used to study human neurological diseases. CSF IgG concentration increases either with neurological diseases that alter the blood-brain barrier (BBB), increasing IgG access from the blood, or with neurological diseases that determine local activation of the immune system with intrathecal synthesis (IS) of IgG. To date, no studies on IEF of canine CSF have been performed. The purpose of this study was to investigate IgG patterns of distribution in CSF of dogs with several neurological diseases and to compare these data to CSF total protein concentrations for each dog.. MATERIALS. Twenty-four dogs have been included in the study. Sixteen dogs had spinal cord compression (SCC), 3 dogs had brain neoplasia (BN), 2 dogs had cerebellar abiotrophy (CA), 1 dog had steroid-responsive meningitis ...
Suspended basket system for freezers and portable coolers - The present invention suspended basket system for freezers and portable coolers, includes: (a) an insulating member selected from the group consisting of freezers and portable coolers, the insulating member including a bottom, a back, an open top, and a cover connectable to an outer perimeter of the open top through cover connectable means; (b) a basket hanging member including an open frame nested to the insulating member through open frame nesting means, at least one hinge point rod connected to the open frame, and a suspension mechanism connected to the at least one hinge point rod through suspension mechanism connecting means, the suspension mechanism further connected to at least one basket through at least one basket connecting means wherein the at least one basket is adapted for holding food and beverages normally stored in the insulating member; such that as the open frame is increasingly opened by lifting the open frame ...
ampholyte answers are found in the Tabers Medical Dictionary powered by Unbound Medicine. Available for iPhone, iPad, Android, and Web.
SERVA IEF sample buffer is suited to all vertical and horizontal IEF applications and systems. The sample buffer is supplied as 2x concentrate. It is sterile filtered, beneficial to long shelf life and absence of contaminants. Simply mix the liquid sample 1:1 with the buffer or dissolve a solid sample in the buffer first and dilute with water 1:1. When performing IEF in the presence of urea mix the sample with the buffer and add solid urea or use concentrated urea solution.The buffer contains 4 % SERVALYT™ 4 - 9 T, 30 % glycerol and 0.005 % phenol red.
Such mixtures of amphoteric Electrolytes or Buffers that provide a continuous range of pH in an electric field; used for separating Proteins by their Isoelectric Points, i.e., by Isoelectric Focusing ...
Reactivity: Chicken, Cow, Dog and more. Compare different PI3K p85a ELISA Kits & buy the right one directly at antibodies-online.com!
Ultrasound Quarterly, the official journal of the Society of Radiologists in Ultrasound, provides coverage of the newest, most sophisticated ultrasound techniques as well as in-depth analysis of important developments in this dynamic field.
The data on approximate values of isoelectric point (pI) of peptides obtained during their fractionation by isoelectric focusing can be successfully used for the creation of the pKa scale for amino ac
bes ,bes at pi5246.physik.uni-erlangen.de, wrote in message news:94oi30$eg5 at smc.vnet.net... , Hallo, , is it possible to use fractions for a label? , Show[Plot[x,{x,-1,1},DisplayFunction-,Identity], Ticks-,{ {-1,-3/4,-1/2,-1/4,1/4,1/2,3/4,1}, {-1,-3/4,-1/2,-1/4,1/4,1/2,3/4,1} } ,DisplayFunction-,$DisplayFunction] -- Paul Lutus www.arachnoid.com ...
You have been walking for years, but chances are, you havent been utilising all of your muscles. Power walking, also known as speed walking, is a cross between regular walking and jogging. An extreme form of power walking-also called race walking-involves rigorous rules and requirements during timed events. Research suggests that power walking or brisk walking can help reduce the risk of heart disease, stroke, type 2 diabetes, cancer and depression. Although it is a very basic form of exercise, it does require some amount of preparation. Here are a few effective ways to maximise the potential of your power walk. ...
BTW, on a side note. I visited a couple of faucet factories in GD recently. Basically the same process. Brass casting the basic form, machining to form flat surfaces for seals, tapping the threads for assembly, polishing the exterior for a smooth finish. Its not that complicated (at least when you are chroming brass ...
CryoBioPhysica, creators of pISep buffers & pISep Software for separation of proteins by chromatofocusing with controllable external pH gradients.
pI profiles across HC CDR3 regions of NZM2410-derived ANAs and non-ANAs. The mean pI value (isoelectric point) at each HC CDR3 position (from H95 to H100a) was
Both SDS-PAGE (sodium dodecyl sulphate polyacrylamide gel electrophoresis) and Western blotting are vital and very common methodology for protein extraction and ...
The female reproductive system of Ceratitis capitata has a pair of accessory glands whose structure has been recently studied. Their epithelium consists of a flattened layer of cells, secretory cells and duct-forming cells, lined by a thin cuticle. Analysis of the secretion reveals the presence of proteins and polysaccharides with a range of 0.5 to 1 µg total protein per gland. SDS-PAGE electrophoresis of the secretion shows several major bands comprised between 13 and 38 kDa. Two-dimensional electrophoresis gives several isoelectric forms concentrated within two main groups: one focalizing between pH 4.5-5.5 and the other between pH 5-5-6.5. Monodimensional isoelectric focusing and anion exchange chromatography of native proteins reveal two classes of proteins distributed at acidic and neutral pH ranges, respectively. Alkaline phosphatases and N-acetyl-ß-D-galactosaminidases have been detected in the secretion. The results relative to the latter enzyme are in line with previous evidence that ...
Mouse lymphocyte H-2 and Ia glycoproteins have been analyzed with a two-dimensional (2-D) acrylamide gel electrophoresis technique, in which proteins are separated first according to their charge in isoelectrofocusing gels and then according to their size in sodium dodecyl sulfate gels. Individual polypeptide chains from radiolabeled cells are resolved as discrete spots on autoradiograms of the gels, forming patterns which are characteristic of the proteins in the sample. 2-D gels of H-2K, H-2D, and Ia glycoproteins immunoprecipitated from 35S-methionine-labeled cells reveal that these proteins exist in the cells as complex arrays of molecules heterogeneous in both size and charge. Lactoperoxidase-catalyzed radioiodination of lymphocyte surfaces labels only subsets of the total H-2 and Ia molecules with 125I, indicating that some of the molecules may represent cytoplasmic precursors of the cell surface proteins. This theory is supported by the kinetics of labeling of various spots in ...
Study participants. Acute infection studies: 5 healthy, HIV-negative individuals received live-attenuated YFV-17D vaccine (YF-Vax, Sanofi Pasteur) and 7 different healthy, HIV-negative individuals received live vaccinia smallpox vaccine (Dryvax, Wyeth Laboratories). Peripheral blood mononuclear cell (PBMC) samples were obtained on or near day of vaccination and at several subsequent time points (see Table 1 for donor information). Seven acutely infected HIV+ donors were enrolled in the RV217 Early Capture HIV Cohort run by the US Military HIV Research Program. PBMC sample timing was calculated relative to first positive HIV RNA test (Abbot Real-Time HIV-1 assay, Abbot Laboratories). Samples included a preinfection time point (ranging 478-41 days previous to first positive HIV RNA test), an early acute infection time point (ranging 1-21 days after first positive test; these were collected during or shortly after peak acute viremia and are considered to represent an early acute infection time ...
Two-dimensional gel electrophoresis of nuclear phosphoproteins of Novikoff hepatoma and regenerating liver.: Two-dimensional polyacrylamide gel electrophoresis
If you have an antibody against the protein you investigate you can perform 1D IEF gels followed by western blots (see my current post). The blotting effiency is not very high because the proteins in the gel are not charged but I try to optimize (washing with SDS). With this method you can detect different phosphorylated forms of that protein. And if you have a suggestion about the respective kinase and are able to construct a mutant (Im working with bacteria...) the phosphorylated band should disapear if your suggestion is right ...
Where are the most interesting and rewarding jobs in Neuroscience, outside of the clinic?. Neuroscience, being a dynamic field and a hot topic at the moment, probably already creates interesting career prospects which do not immediately come to mind when thinking about the field. It would be great to hear from neuroscientists who work in the…[Read more] ...
When you are 3 weeks pregnant, your 100-cell blastocyst would be the highlight of your show. This is the basic form of life which would later form a baby.
hithankyou for your reply. great article.this is as you said a bit high tech and would involve sensors and stuff and accurate calibrationsi am currently thinking of making the most basic form of ventillator there can be. essentially a pump with stroke volume of around 450/stroke, and a veriable pressere valve with range around 10-20mmhg thats it. also it needs constant +4mmhg of pressure in tubes.as one says that something is better than nothing. so i am thinking of excluding the fancy modes.my rationelle is that the patient will be completely sedated with medicines so will only be dependent on machine (no breaths of his own) so that this machine can be used temporarily. does this makes sense.thankyou for your help ...
How to Slide Tackle in Soccer. There are a number of tackling options in soccer and the slide tackle is considered the riskiest and flashiest, but it can make for an incredible play if performed properly. The basic form includes sliding on...
Entrepreneurial Ecosystems around the World By Zoltan Acs Over the past dozen years we have witnessed a new approach to improving the worlds economy.The story goes something like this. If we could get more cooperation and integration between economies then the economy might improve. The answer seems to be found in something called an entrepreneurial…
Entrepreneurial Ecosystems around the World By Zoltan Acs Over the past dozen years we have witnessed a new approach to improving the worlds economy.The story goes something like this. If we could get more cooperation and integration between economies then the economy might improve. The answer seems to be found in something called an entrepreneurial…
"Chapter 2 Evolution and development of isoelectric focusing". In Garfin, David; Ahuja, Satinder (eds.). Handbook of isoelectric ... Righetti, P. G.; Drysdale, J. W. (1976). Isoelectric focusing. New York: American Elsevier Pub. Co. 2014, AES Award, American ... They developed techniques for isoelectric focusing (IEF) using soluble carrier ampholytes and polyacrylamide gels. In 1971, ... Righetti, P. G. (1983). Isoelectric focusing : theory, methodology, and applications. 1st ed. Laboratory techniques in ...
The aim of isoelectric focusing (IEF), for example, is to separate proteins according to their isoelectric point (pI), thus, ... Garfin DE (1990). Isoelectric focusing. Methods in Enzymology. 182. pp. 459-77. doi:10.1016/0076-6879(90)82037-3. ISBN ... Chapters on 1-D / 2-D Gel Electrophoresis, and Isoelectric Focusing are provided by AES Electrophoresis Society Learning Center ... Under standard conditions (69 hr), metalloproteins with different molecular mass ranges and isoelectric points have been ...
Reinhart and Malamud; Malamud, D (1982). "Protein transfer from isoelectric focusing gels: the native blot". Analytical ...
IPG increased reproducibility of Isoelectric focusing and 2D-Gel Electrophoresis. Other advantages are increased resolution, ... of protein spots in two-dimensional polyacrylamide gel electrophoresis using immobilised pH gradient isoelectric focusing in ... The gel is then washed to remove catalysts and unpolymerized monomers, which interfere with isoelectric separation. ...
After that, the resulting solution was fractionated using standard procedures and generated, after isoelectric focusing, a ... This value was obtained with isoelectric focusing and chromatofocusing techniques. The enzyme broad temperature range is ... The BBE-like enzyme's optimum pH is 8.9, this means it works in an alkaline medium, but the isoelectric point of the ...
The first-dimension of 2D gel is isoelectric focusing (IEF). In this dimension, the protein is separated by its isoelectric ...
"Fractionation of complex protein mixtures by liquid-phase isoelectric focusing". 2D PAGE: Sample Preparation and Fractionation ... ISBN 978-0-19-963789-8. Kozlowski LP (January 2017). "Proteome-pI: proteome isoelectric point database". Nucleic Acids Research ... can be monitored using various types of gel electrophoresis if the desired protein's molecular weight and isoelectric point are ...
Klose J (1975). "Protein mapping by combined isoelectric focusing and electrophoresis of mouse tissues. A novel approach to ... It can be used in projects ranging from large-scale whole proteome to focusing on a single protein family. It can be done in ...
1995). "Purification of ginger proteases by DEAE-Sepharose and isoelectric focusing". Biochim. Biophys. Acta. 1243 (2): 181-4. ...
Wang S, Chen S, Wang J, Xu P, Luo Y, Nie Z, Du W (September 2014). "Interface solution isoelectric focusing with in situ MALDI- ... Flow focusing can also be an active method with the constraint point being adjustable using pneumatic side chambers controlled ... Using specific flow-focusing multiphase designs that control flow rate and temperature, the size of nanoparticle formation can ... Hatch AC, Patel A, Beer NR, Lee AP (April 2013). "Passive droplet sorting using viscoelastic flow focusing". Lab on a Chip. 13 ...
ACES use in isoelectric focusing of proteins has also been documented. Use of ACES has been published in a protocol for the ... Alonso, A., "Human α-1-antitrypsin subtyping by hybrid isoelectric focusing in miniaturized polyacrylamide gel." " ...
Separation of the proteins by isoelectric point is called isoelectric focusing (IEF). Thereby, a pH gradient is applied to a ... In addition, proteins will not migrate when they have no charge (a result of the isoelectric focusing step) therefore the ... See Isoelectric focusing In quantitative proteomics, these tools primarily analyze bio-markers by quantifying individual ... Klose, J (1975). "Protein mapping by combined isoelectric focusing and electrophoresis of mouse tissues. A novel approach to ...
Williams RC, Shah C, Sackett D (November 1999). "Separation of tubulin isoforms by isoelectric focusing in immobilized pH ... These subunits are slightly acidic with an isoelectric point between 5.2 and 5.8. Each has a molecular weight of approximately ...
... comparison of three isoelectric focusing methods". Protein Expression and Purification. 9 (1): 76-82. doi:10.1006/prep. ...
As protein electrophoresis is imprecise, the A1AT phenotype is analysed by isoelectric focusing (IEF) in the pH range 4.5-5.5, ... this causes the heterogeneity observed on normal A1AT when analysed by isoelectric focusing. Also, the fucosylated triantennary ... where the protein migrates in a gel according to its isoelectric point or charge in a pH gradient. Normal A1AT is termed M, as ...
Her thesis is titled "Isoelectric Focusing for Multi-Dimensional Separations in Microfluidic Devices."[citation needed] After ...
250, 4007-4021 (1974). Klose, J. Protein mapping by combined isoelectric focusing and electrophoresis of mouse tissues. A novel ...
ATTR can be identified using isoelectric focusing which separates mutated forms of transthyretin. Findings can be corroborated ...
... so different molecules have different isoelectric points. This permits a technique called isoelectric focusing,[74] which is ... Isoelectric point[edit]. Main article: isoelectric point. For substances in solution, the isoelectric point (pI) is defined as ... 2005). Handbook of Isoelectric Focusing and Proteomics. 7. Elsevier. ISBN 0-12-088752-5. .. ... At the isoelectric point the concentration of the positively charged species, AH2+, is equal to the concentration of the ...
Ampholytes are used to establish a stable pH gradient for use in isoelectric focusing. Amphoteric is derived from the Greek ... The pH at which the average charge is zero is known as the molecule's isoelectric point. ... Zwitterion Isoelectric point Ate complex IUPAC, Compendium of Chemical Terminology, 2nd ed. (the "Gold Book") (1997). Online ...
Isoelectric focusing is the separation of proteins, organelles, and cells with different isoelectric points. Isoelectric ... Examples of continuous-flow separation techniques include continuous-flow electrophoresis, isoelectric focusing, continuous- ... Sorting and focusing of the species of interest is achieved because an electrophoretic force causes perpendicular migration ... Thus, companies focusing on tropical diseases must often combine their research objectives for tropical disease with research ...
1987). "Genetic study of red cell esterase D polymorphism by ultrathin layer isoelectric focusing. Distribution in the Veneto ...
Two-dimensional gel electrophoresis sequentially combines isoelectric focusing or BAC-PAGE with a SDS-PAGE. Native PAGE is used ...
... s are prolamins and are separated on the basis of electrophoretic mobility and isoelectric focusing. Gliadin peptides ...
"Characterization of human exocrine pancreatic proteins by two-dimensional isoelectric focusing/sodium dodecyl sulfate gel ...
"Fractionation of complex protein mixtures by liquid-phase isoelectric focusing". Methods in Molecular Biology. Methods in ... "Proteome-pI: proteome isoelectric point database". Nucleic Acids Research. 45 (D1): D1112-D1116. ISSN 0305-1048. PMC 5210655 ... can be monitored using various types of gel electrophoresis if the desired protein's molecular weight and isoelectric point are ...
New techniques like "capillary isoelectric focusing immunoassay" are able to detect IgG OCBs in more than 95% of multiple ... the combination of isoelectric focusing/silver staining. The latter is more sensitive. For the analysis of cerebrospinal fluid ... "Detection of intrathecal immunoglobulin G synthesis by capillary isoelectric focusing immunoassay in oligoclonal band negative ... of virus-specific oligoclonal bands in subacute sclerosing panencephalitis by immunofixation after isoelectric focusing and ...
After separation, an additional separation method may then be used, such as isoelectric focusing or SDS-PAGE. The gel will then ... 2D gel analysis software History of electrophoresis Electrophoretic mobility shift assay Gel extraction Isoelectric focusing ... isoelectric focusing then SDS gel electrophoresis 1977 - sequencing gels 1983 - pulsed field gel electrophoresis enables ... isoelectric histidine, pK matched goods buffers, etc.; in most cases the purported rationale is lower current (less heat) ...
Two parameters were used: isoelectric focusing in the first dimension sodium dodecyl sulfate electrophoresis in the second ... This permitted the simultaneous determination of molecular weight and isoelectric point for the proteins. Because the two ...
It started with an image of an IPG-IEF (isoelectric focusing) gel selection that was then analyzed by MALDI. Top-down ... Study Two: Combining high-throughput MALDI-TOF mass spectrometry and isoelectric focusing gel electrophoresis for virtual 2D ... "Combining high-throughput MALDI-TOF mass spectrometry and isoelectric focusing gel electrophoresis for virtual 2D gel-based ... MALDI is a computer software that generates the intact masses of the proteins at each isoelectric point. ...
Fractionation of complex protein mixtures by liquid-phase isoelectric focusing. Methods in Molecular Biology. Methods in ... "Proteome-pI: proteome isoelectric point database". Nucleic Acids Research. 45 (D1): D1112-D1116. doi:10.1093/nar/gkw978. PMC ... can be monitored using various types of gel electrophoresis if the desired protein's molecular weight and isoelectric point are ...
Although the phenomenon we now call dielectrophoresis was described in passing as far back as the early 20th century, it was only subject to serious study, named and first understood by Herbert Pohl in the 1950s.[9][10] Recently, dielectrophoresis has been revived due to its potential in the manipulation of microparticles,[2][4][5][11]nanoparticles and cells. Dielectrophoresis occurs when a polarizable particle is suspended in a non-uniform electric field. The electric field polarizes the particle, and the poles then experience a force along the field lines, which can be either attractive or repulsive according to the orientation on the dipole. Since the field is non-uniform, the pole experiencing the greatest electric field will dominate over the other, and the particle will move. The orientation of the dipole is dependent on the relative polarizability of the particle and medium, in accordance with Maxwell-Wagner-Sillars polarization. Since the direction of the force is dependent on field ...
... field amplified sample stacking and isoelectric focusing), multidimensional assays, and systems with poorly specified sample ...
In the first dimension, the proteins are separated by isoelectric focusing, which resolves proteins on the basis of charge. In ... "Proteome-pI: proteome isoelectric point database". Nucleic Acids Research. 45 (D1): D1112-D1116. doi:10.1093/nar/gkw978. PMC ...
Ampholytes are used to establish a stable pH gradient for use in isoelectric focusing. ... The pH at which the average charge is zero is known as the molecule's isoelectric point. ...
... so different molecules have different isoelectric points. This permits a technique called isoelectric focusing,[68] which is ... Isoelectric pointEdit. Main article: isoelectric point. For substances in solution the isoelectric point (pI) is defined as the ... 2005). Handbook of Isoelectric Focusing and Proteomics. 7. Elsevier. ISBN 0-12-088752-5.. ... At the isoelectric point the concentration of the positively charged species, AH2+, is equal to the concentration of the ...
By purifying urine using salt precipitation, isoelectric focusing, and size-exclusion chromatography, some researchers have ...
The immunoglobulins or antibodies are generally the only proteins present in the normal gamma region. Of note, any protein migrating in the gamma region will be stained and appear on the gel, which may include protein contaminants, artifacts, or certain medications. Depending on whether an agarose or capillary method is used, interferences vary. Immunoglobulins consist of heavy chains (IgA, IgM, IgG, IgE and IgD) and light chains (kappa and lambda). A normal gamma zone should appear as a smooth 'blush', or smear, with no asymmetry or sharp peaks.[12] The gamma globulins may be elevated (hypergammaglobulinemia), decreased (hypogammaglobulinaemia), or have an abnormal peak or peaks. Note that immunoglobulins may also be found in other zones; IgA typically migrates in the beta-gamma zone, and in particular, pathogenic immunoglobulins may migrate anywhere, including the alpha regions. Hypogammaglobulinaemia is easily identifiable as a "slump" or decrease in the gamma zone. It is normal in infants. ...
Isoelectric focusing. *Isotachophoresis. *Native gel electrophoresis. *Northern blotting. *Protein electrophoresis. *Southern ... as each protein has an isoelectric point and molecular weight particular to its primary structure. This is known as native PAGE ...
Studies focusing on the accumulation and loss of subunits by microfilaments are carried out in vitro (that is, in the ... has a molecular mass of 41,785 Da and an estimated isoelectric point of 4.8. Its net charge at pH = 7 is -7.[15][31] ... according to their isoelectric points. In general, alpha actins are found in muscle (α-skeletal, α-aortic smooth, α-cardiac), ...
... and for isoelectric focusing in immobilized pH gradients. Its effects on dog kidney Na+/K+-ATPase and rat brain GABA receptors ...
C1orf112 has a determined precursor molecular weight of 96.6 kDa and an isoelectric point of 5.62. C1orf112 has been ... "The focusing positions of polypeptides in immobilized pH gradients can be predicted from their amino acid sequences". ...
The most commonly used screening method for CDG, analysis of transferrin glycosylation status by isoelectric focusing, ESI-MS, ... isoelectric focusing), the new syndrome was named carbohydrate-deficient glycoprotein syndrome (CDGS) Its "classical" phenotype ...
The binary shifts might be left to right (change in isoelectric point), vertical (change in size) or diagonal (change in both ... The three samples are mixed and loaded onto IEF (isolectric focusing chromatography) for first dimension and the strip is ... truncations and any modification that might change the size or isoelectric point of proteins. ... size and isoelectric point). Reciprocal Labeling is done to make sure the changes seen are not due to dye-dependent ...
... including capillary isotachophoresis and capillary isoelectric focusing. There are two common techniques to load the sample ...
Kobayashi T, Yang X, Walker LA, Van Breemen RB, Solaro RJ (Jan 2005). "A non-equilibrium isoelectric focusing method to ...
MATRICE OF Spirulina platensis Determination of the pI of Human Rhinovirus Serotype 2 by Capillary Isoelectric Focusing The ...
July 2000 Isoelectric Focusing. 1975. The Determinants of Bacterial and Viral Pathogenicity. 1983. Foodborne Illness: a Lancet ...
... for a large number of hemoglobinopathies is done by detecting abnormal patterns using isoelectric focusing, ...
In the first dimension, the proteins are separated by isoelectric focusing, which resolves proteins on the basis of charge. In ... Kozlowski, LP (26 October 2016). "Proteome-pI: proteome isoelectric point database". Nucleic Acids Research. 45 (D1): D1112- ...
Isoelectric focusing can resolve proteins that differ in pI value by as little as 0.01. Isoelectric focusing is the first step ... "Multijunction Capillary Isoelectric Focusing Device Combined with Online Membrane-Assisted Buffer Exchanger Enables Isoelectric ... Isoelectric focusing (IEF), also known as electrofocusing, is a technique for separating different molecules by differences in ... According to some opinions, living eukaryotic cells perform isoelectric focusing of proteins in their interior to overcome a ...
... obtained with an analytical isoelectric focusing technique, in... ... Isoelectric focusing of PGM1 (E.C.2.7.5.1) on agarose. ... Isoelectric focusing of red cell phosphoglucomutase (E.C.:2.7.5.1) at the PGM1 locus in a French-Canadian population. Human ... Isoelectric focusing studies of human red cell PGM1 in Japanese, with special reference to the characterization of PGM1*7. ... Electrophoretic and isoelectric focusing studies in Brazilian Indians: data on four systems. Human Biology, 61: 427-438.Google ...
Associations of Isoelectric Focusing with chemical compounds. *Isoelectric focusing in agarose, and two-dimensional ... Gene context of Isoelectric Focusing. *We confirmed the presence of mutant GH in the serum using isoelectric focusing analysis ... Disease relevance of Isoelectric Focusing. *We have now used isoelectric focusing (IEF) to compare IgG eluted from control ... Isoelectric focusing of IgG eluted from multiple sclerosis and subacute sclerosing panencephalitis brains. Mattson, D.H., Roos ...
R factor-mediated enzymes gave identical isoelectric focusing patterns irrespective of the host strain. Isoelectric focusing ... The use of analytical isoelectric focusing for detection and identification of beta-lactamases.. Mathew A, Harris AM, Marshall ... 3.5.2.6) from strains of Gram-negative bacteria have been studied using analytical isoelectric focusing. This permits a visual ... Conversely, the enzymes specified by the R factors RTEM, R1 and RGN14, with identical isoelectric focusing patterns have the ...
The 3100 OFFGEL Fractionator is used for the separation and fractionation of proteins and peptides by isoelectric focusing (IEF ... The 3100 OFFGEL Fractionator is used for the separation and fractionation of proteins and peptides by isoelectric focusing (IEF ... separate proteins by isoelectric focusing as the first dimension in classical 2-dimesnional gel electrophoresis. It can be ...
Is there anybody to help me for my Isoelectic focusing stage. Ive ran 5 strips (7cm, 3-10) but the voltage didnt go up to 500 ... Isoelectric focusing - posted in Protein and Proteomics: Hi, ... Isoelectric focusing. Started by Etemadi, Mar 03 2013 04:18 AM ... Is there anybody to help me for my Isoelectic focusing stage. Ive ran 5 strips (7cm, 3-10) but the voltage didnt go up to 500 ...
It has now been over a decade since isoelectric focusing became estab- lished as a valuable addition... ... Biological and Biomedical Applications of Isoelectric Focusing by Catsimpoolas, Nicholas available in Trade Paperback on ... It has now been over a decade since isoelectric focusing became estab- lished as a valuable addition to our arsenal of physical ... Conse- quently, we considered it desirable to review some of the important appli- cations of the isoelectric focusing technique ...
IEF Standards, Isoelectric Focusing and Two Dimensional Electrophoresis, Molecular Biology, Molecular Weight Markers, Protein ... Isoelectric focusing marker, pI (1) 6.8, (2) 7.2 Synonym: Myoglobin from horse heart ... Trypsin inhibitor from Glycine max (soybean), Isoelectric focusing marker, pI 4.6 pricing ... Myoglobin from equine heart is a protein which is used as a standard pI marker for isoelectric focussing experiments. ...
chips for isoelectric focusing and zone electrophoresis in the free- flow mode Yi Xu, Chao-Xuan Zhang, Andreas Manz Imperial ... chips for isoelectric focusing and zone electrophoresis in the free-flow mode. ... talk given at MSB 2003, New Orleans. ... chips for isoelectric focusing and zone electrophoresis in the free-flow mode ... isoelectric focusing (IEF) • establish pH gradient • establish electric field • charge of protein depends on pH • mobility is ...
3.5.2.6) from strains of Gram-negative bacteria have been studied using analytical isoelectric focusing. This permits a visual ... R factor-mediated enzymes gave identical isoelectric focusing patterns irrespective of the host strain. Isoelectric focusing ... The use of analytical isoelectric focusing for detection and identification of beta-lactamases J Gen Microbiol. 1975 May;88(1): ... 3.5.2.6) from strains of Gram-negative bacteria have been studied using analytical isoelectric focusing. This permits a visual ...
J C Waldrep, R L Noe, R D Stulting; Analysis of human corneal IgG by isoelectric focusing.. Invest. Ophthalmol. Vis. Sci. 1988; ... Isoelectric focusing (IEF) and blotting analysis of IgG revealed a restricted pI profile for both central and peripheral ... Analysis of human corneal IgG by isoelectric focusing. You will receive an email whenever this article is corrected, updated, ... Analysis of aqueous humor immunoglobulin G in uveitis by enzyme-linked immunosorbent assay, isoelectric focusing, and ...
The prepared reagent-release hydrogels showed some advantages for isoelectric focusing, e.g., high durability for at least five ... The migration behavior of ions from the hydrogels into the capillary and the formation of a pH gradient in isoelectric focusing ... In this study, reagent-release hydrogels containing an acid or a base solution were developed for isoelectric focusing using ... In conventional isoelectric focusing techniques using microfluidic channels, complicated liquid manipulations are required, ...
Free-flow isoelectric focusing. To realize the isoelectric focusing in a microfluidic chip, syringe pumps (cetoni neMESYS low ... Continuous on-chip fluorescence labelling, free-flow isoelectric focusing and marker-free isoelectric point determination of ... their free-flow isoelectric focusing, observation of the pH gradient and assignment of isoelectric points (pI) based on an ... their separation via free-flow isoelectric focusing and the determination of the isoelectric point (pI) of the separated ...
G-Biosciences offer a large selection of 2D gel electrophoresis (2DGE) and IEF optimized reagents for the crucial preparation of native protein samples. We provide 2DGE sample clean up to ensure high quality spot resolution. We offer mass spectrometry co
This methodology employs isoelectric focusing of peptides on immobilized, pH gradient (IPG) gels in the first dimension, ... This methodology employs isoelectric focusing of peptides on immobilized, pH gradient (IPG) gels in the first dimension, ... A comparison of immobilized pH gradient isoelectric focusing and strong-cation-exchange chromatography as a first dimension in ... Essader, A., Cargile, B., Bundy, J., & Stephenson, J. (2005). A comparison of immobilized pH gradient isoelectric focusing and ...
This report describes the fabrication and characterization of a simple and disposable capillary isoelectric focusing (cIEF) ... isoelectric focusing. device as a rapid screening device for IEF. condition optimization M. Kataoka, H. Yokoyama, T. G. Henares ... capillary isoelectric focusing. (. cIEF. ) device containing a reagent-release capillary (RRC) array and poly(dimethylsiloxane ... isoelectric focusing. device as a rapid screening device for IEF. condition optimization ...
Isoelectric Focusing (IEF) can be described as an ingenious process for simultaneous concentration and separation of proteins. ... in the order of their isoelectric points.. For IEF, SERVA offers a comprehensive product line:* SERVALYT™ Carrier Ampholytes* ... isoelectric point). IEF is an end point method - when the electrophoretic run is completed proteins will appear as separate, ...
This chapter describes the use of preparative denaturing isoelectric focusing to enrich for proteins of specific isoelectric ... Proteomics 2D gel preparative isoelectric focusing phosphoproteomics This is a preview of subscription content, log in to check ... Serna-Sanz A., Rairdan G., Peck S.C. (2007) Preparative Denaturing Isoelectric Focusing for Enhancing Sensitivity of Proteomic ...
Looking for abbreviations of AGIEF? It is Agarose Gel Isoelectric Focusing. Agarose Gel Isoelectric Focusing listed as AGIEF ... Agarose Gel Isoelectric Focusing - How is Agarose Gel Isoelectric Focusing abbreviated? https://acronyms.thefreedictionary.com/ ... a href=https://acronyms.thefreedictionary.com/Agarose+Gel+Isoelectric+Focusing,AGIEF,/a,. *Facebook ... redirected from Agarose Gel Isoelectric Focusing) Acronym. Definition. AGIEF. Agarose Gel Isoelectric Focusing. ...
Looking for abbreviations of TLIF? It is Thin Layer Isoelectric Focusing. Thin Layer Isoelectric Focusing listed as TLIF ... Thin Layer Isoelectric Focusing - How is Thin Layer Isoelectric Focusing abbreviated? https://acronyms.thefreedictionary.com/ ... a href=https://acronyms.thefreedictionary.com/Thin+Layer+Isoelectric+Focusing,TLIF,/a,. *Facebook ...
Detection of IgG Oligoclonal Bands in Unconcentrated CSF by Isoelectric Focusing in Ultrathin Polyacrylamide Gel, Direct ...
ready-to-use protein markers for isoelectric focusing* one standard applicable to all IEF gels (vertical/horizontal)* purified ... To determine the isoelectric points of unknown proteins the pH of focused bands may be measured on the gel using a surface ...
The measurement of the intrinsic alkaline Bohr effect of various human haemoglobins by isoelectric focusing. Claude F. Poyart, ... We have used isoelectric focusing to measure the differences between the pI values of various normal and mutant human ... The variation of the ΔpH(deox.-ox.) curve as a function of pH (ox.) was similar to the isoelectric-focusing curve relating the ... The measurement of the intrinsic alkaline Bohr effect of various human haemoglobins by isoelectric focusing ...
Isoelectric focusing. es_ES. dc.title. Specific enzyme detection following isoelectric focusing as a complimentary tool for the ... enzymes in the sarcoplasmic protein fraction of eight gadoid fish species has been investigated following isoelectric focusing ...
Preparative liquid isoelectric focusing (Rotofor IEF) based Se-speciation of Se-enriched Agaricus bisporus. Publication: ... Preparative isoelectric focusing ( Rotofor) was introduced to carry out the prefractionation of a selenised edible mushroom ...
For all samples analyzed, data obtained by capillary isoelectric focusing could be confirmed with gel isoelectric focusing. The ... For all samples analyzed, data obtained by capillary isoelectric focusing could be confirmed with gel isoelectric focusing. The ... a double peak in capillary isoelectric focusing only and a double peak in capillary zone electrophoresis only. ... a double peak in capillary isoelectric focusing only and a double peak in capillary zone electrophoresis only. ...
"USE OF A MODIFIED UREA GEL ISOELECTRIC FOCUSING METHOD FOR SPECIES IDENTIFICATION OF RAW OR BOILED WHITE, PINK, AND ROCK SHRIMP ... USE OF A MODIFIED UREA GEL ISOELECTRIC FOCUSING METHOD FOR SPECIES IDENTIFICATION OF RAW OR... WEI, CHENG‐I; AN, HAEJUNG; CHEN ... ABSTRACT Isoelectric focusing (IEF) polyacrylamide gel containing an 80% pH 4-6.5 and 20% pH 3-10 ampholyte mixture greatly ... USE OF A MODIFIED UREA GEL ISOELECTRIC FOCUSING METHOD FOR SPECIES IDENTIFICATION OF RAW OR BOILED WHITE, PINK, AND ROCK SHRIMP ...
mmunoglobulin A, isoelectric focusing, cerebrospinal fluid Resumen. Motivation: Multiple sclerosis (MS) is characterized by ... Methods: Isoelectric focusing is one of the most powerful techniques used to solve complex mixtures of proteins [2]. ... Sidén, and E. Ösby, "Isoelectric focusing of monoclonal immunoglobulin G, A and M followed by detection with the avidin‐biotin ... 4] K. B. Elkon, "Isoelectric focusing of human IgA and secretory proteins using thin layer agarose gels and nitrocellulose ...
Isoelectric Focusing Separation of AFP. The mixed dialysed protein with ampholytes was subjected to an isoelectric focusing ... Optimization of AFP Purification from A. giganteus by Isoelectric Focusing Technique. The isoelectric focusing (IEF) is a ... Comparing to mentioned purification protocol by [2] [5] -[8] [34] , the isoelectric focusing could be 5 days shorter in time ... A liquid isoelectric focusing using BioRads Rotofor cell (Figure 1), which separate the sample into 20 fractions was used. To ...
Anion-exchange chromatography versus isoelectric focusing of transferrin in diagnosing the carbohydrate-deficient glycoprotein ...
  • Isoelectric focusing is the first step in two-dimensional gel electrophoresis, in which proteins are first separated by their pI value and then further separated by molecular weight through SDS-PAGE. (wikipedia.org)
  • The instrument can be used to (i) fractionate proteins and peptides prior to downstream analytical methods such as liquid chromatography coupled mass spectrometry (LC-MS), (ii) purify peptides, proteins or protein complexes and (iii) separate proteins by isoelectric focusing as the first dimension in classical 2-dimesnional gel electrophoresis. (otago.ac.nz)
  • Asialo-transferrin forms are analyzed by capillary zone electrophoresis using assay conditions as for the monitoring of carbohydrate-deficient transferrin or by capillary isoelectric focusing in a pH 5-8 gradient which requires immunoextraction of transferrin prior to analysis. (uzh.ch)
  • Monitoring of CC patterns revealed three cases, namely those producing double peaks in both methods, a double peak in capillary isoelectric focusing only and a double peak in capillary zone electrophoresis only. (uzh.ch)
  • Isoelectric focusing (IEF) has been traditionally used as a first-dimension protein separation technique in two-dimensional gel electrophoresis-based proteomics. (scilifelab.se)
  • multicompartment electrolyzers for isoelectric membranes and membrane‐trapped enzyme reactors, and temperature‐programmed capillary zone electrophoresis (CZE). (wikipedia.org)
  • Isoelectric focusing is also the first step in 2-D gel polyacrylamide gel electrophoresis . (princeton.edu)
  • We increased the number of peptide and protein ion signals observed in the MALDI-TOF mass spectra analysis of human serum by using a prefractionation protocol based on liquid phase isoelectric focusing electrophoresis. (duke.edu)
  • The labeled peptide was isolated by carboxymethylcellulose chromatography or by isoelectric focusing (IEF) or electrophoresis in polyacrylamide gel, and its radioactivity measured. (naver.com)
  • We fractionated the SILAC-labeled ES cell proteome by one-dimensional gel electrophoresis and by isoelectric focusing of peptides. (mcponline.org)
  • This methodology employs isoelectric focusing of peptides on immobilized, pH gradient (IPG) gels in the first dimension, followed by reversed-phase chromatography in the second dimension, and subsequent tandem mass spectrometry analysis. (rti.org)
  • This chapter describes the use of preparative denaturing isoelectric focusing to enrich for proteins of specific isoelectric points before separation by 2D gels. (springer.com)
  • 4] K. B. Elkon, "Isoelectric focusing of human IgA and secretory proteins using thin layer agarose gels and nitrocellulose capillary blotting," J. Immunol. (upo.es)
  • The IEF-SYS horizontal isoelectric focusing system is optimized for IEF applications using IPG Strips and precast horizontal IEF gels . (biophoretics.com)
  • Mouse brain adenylate cyclase has been solubilized with Lubrol PX and separated by isoelectric focusing on polyacrylamide gels. (nyu.edu)
  • SDS-PAGE gels were used to assess the quality of the method which was subsequently tested during isoelectric focusing of 2-DE gels taken from samples of patients with and without the disease. (scielo.br)
  • The new protein extraction protocol does not interfere in isoeletric focusing of 2-DE gels. (scielo.br)
  • The isoelectric points were determined and compared with the values obtained in gels. (microbiologyresearch.org)
  • They developed techniques for isoelectric focusing (IEF) using soluble carrier ampholytes and polyacrylamide gels. (wikipedia.org)
  • This provides a powerful separation of proteins using either of these two gels only, which are isoelectric focusing or SDS-PAGE. (news-medical.net)
  • Mattson, DH , Roos, RP & Arnason, BGW 1980, ' Immunoperoxidase staining of cerebrospinal fluid IgG in isoelectric focusing gels: A sensitive new technique ', Journal of Neuroscience Methods , vol. 3, no. 1, pp. 67-75. (elsevier.com)
  • The pI of purified enzyme was found to be 5.2 by isoelectric focusing on ultrathin polyacrylamide gels. (madsci.org)
  • The genetic types of orosomucoid (ORM) were analyzed by isoelectric focusing (IEF) on polyacrylamide gels and subsequent print lectinofixation with a lectin from the beetle, allo A. In this paper, the newly devised print lectinofixation for ORM typing is described. (elsevier.com)
  • A protein that is in a pH region below its isoelectric point (pI) will be positively charged and so will migrate toward the cathode (negatively charged electrode). (wikipedia.org)
  • Myoglobin from equine heart is a protein which is used as a standard pI marker for isoelectric focussing experiments. (sigmaaldrich.com)
  • Using this assembly, labelling of protein and peptide mixtures, their separation via free-flow isoelectric focusing and the determination of the isoelectric point (pI) of the separated products via the integrated sensor layer could be carried out within typically around 5 minutes. (rsc.org)
  • The presence of specific enzymes in the sarcoplasmic protein fraction of eight gadoid fish species has been investigated following isoelectric focusing (IEF) in the 3.5-9.5 pH range. (csic.es)
  • MARSHALL, MAURICE R. 1990-04-01 00:00:00 ABSTRACT Isoelectric focusing (IEF) polyacrylamide gel containing an 80% pH 4-6.5 and 20% pH 3-10 ampholyte mixture greatly improved protein banding pattern for species identification of water extracts of raw pink, white and rock shrimp compared with the system using only the pH 3-10 range ampholyte. (deepdyve.com)
  • Interestingly, antifungal protein AFP was purified from Aspergillus giganteus supernatants with modified isoelectric focusing procedure after adaptation of the secretion conditions. (scirp.org)
  • Learn how to perform isoelectric focusing and discover protein isoforms you may be missing using only SDS-PAGE. (biophoretics.com)
  • Isoelectric Focusing can provide high resolution of differentially phosphorylated forms of a protein. (bio-protocol.org)
  • Model of generation of an electric field in the cell interior and of an isoelectric focusing of a protein, pH 6.8. (flegr.com)
  • Isoelectric focusing revealed the presence of the mutant apo A-I Fin protein in both serum and HDL of the affected subjects. (ahajournals.org)
  • Photochemical immobilization of proteins onto capillary walls following isoelectric focusing improves protein detection sensitivity. (sciencemag.org)
  • Isoelectric fractionation in protein chemistry]. (naver.com)
  • Description:Isoelectric focusing (IEF) is a high-resolution, stand-alone technique that can be used as an analytical method or tool for protein purification. (pdf-drive.com)
  • Separation of protein by this method is dependent upon balance between the negative charged (acidic) and positive charged (basic) constituent amino acids, which defines a property called protein isoelectric (PI) point. (news-medical.net)
  • In this method, first the protein is passed in a narrow path of isoelectric targeting gel. (news-medical.net)
  • Based on the isoelectric point the protein can be separated. (news-medical.net)
  • The results obtained from three replicate fractionation experiments on the same serum sample indicated that 148 different peptide and protein ion signals were reproducibly detected using our isoelectric focusing and MALDI-TOF MS protocol. (duke.edu)
  • We compared two different preparation approaches, cell fractionation followed by one-dimensional gel separation and in-solution digestion of total cell lysate combined with isoelectric focusing, and found comparable proteome coverage with no apparent bias for any functional protein classes for either approach. (mcponline.org)
  • The 3100 OFFGEL Fractionator is used for the separation and fractionation of proteins and peptides by isoelectric focusing (IEF). (otago.ac.nz)
  • Chp F peptides were found to self-assemble into amyloid fibrils at acidic pH (3.0 or the isoelectric point (pI) of 4.2), with ~99% of peptides converted into insoluble fibrils. (mdpi.com)
  • According to some opinions, living eukaryotic cells perform isoelectric focusing of proteins in their interior to overcome a limitation of the rate of metabolic reaction by diffusion of enzymes and their reactants, and to regulate the rate of particular biochemical processes. (wikipedia.org)
  • Does a cell perform isoelectric focusing? (flegr.com)
  • This paper reports an exhaustive and updated compilation of phenotype and allele frequency data for phosphoglucomutase locus 1 (PGM1), obtained with an analytical isoelectric focusing technique, in human populations. (springer.com)
  • The use of analytical isoelectric focusing for detection and identification of beta-lactamases. (nih.gov)
  • 3.5.2.6) from strains of Gram-negative bacteria have been studied using analytical isoelectric focusing. (nih.gov)
  • In conventional isoelectric focusing techniques using microfluidic channels, complicated liquid manipulations are required, which can decrease analytical performance such as the reproducibility of a focusing position. (go.jp)
  • 3.5.2.6) can be directly compared by analytical isoelectric focusing. (microbiologyresearch.org)
  • Since β -lactamases may be universally produced by bacteria, separation of the enzymes by analytical isoelectric focusing could be used in bacterial taxonomy. (microbiologyresearch.org)
  • Herein, we developed a novel recycling free-flow isoelectric focusing (RFFIEF) with post-pH gradient sample injection (post-PGSI) for preparative separation of mitochondria. (northwestern.edu)
  • Preparative isoelectric focusing ( Rotofor) was introduced to carry out the prefractionation of a selenised edible mushroom Agaricus bisporus sample containing 1037 +/- 31 ( SD) mug Se g(-1). (dtu.dk)
  • We therefore fractionated eggs of the almond moth, Ephestia kuehniella Zeller by preparative isoelectric focusing and bioassayed the resultant fractions in test diets. (bioone.org)
  • To investigate the predictive accuracy of using a combination of the high pressure liquid chromatography [ HPLC ] retention time and the relative isoelectric focusing [IEF] position to diagnose rare hemoglobin variants. (bvsalud.org)
  • Separation Science and Technology 7Handbook of Isoelectric Focusing and Proteomics [1ed. (pdf-drive.com)
  • The only current book on the market, the Handbook of Isoelectric Focusing and Proteomics is the ideal 'one-stop' source for germane information in this discipline. (pdf-drive.com)
  • Complete with the history of IEF focusing to authors' insights and practical tips, this book is a must for anyone working in proteomics. (pdf-drive.com)
  • Summary of cases evaluated by serum transferrin isoelectric focusing at the Laboratory of Inborn Errors of Metabolism of Hospital de Clínicas de Porto Alegre, Brazil (2008-2017). (elsevier.es)
  • To characterize cases of suspected congenital disorders of glycosylation (CDG) investigated in a laboratory in southern Brazil using the transferrin isoelectric focusing TfIEF test from 2008 to 2017. (elsevier.es)
  • We compared the performance of two highly resolving methods, chromatofocusing (CRF) and isoelectric focusing in immobilized pH gradients (IPGF), for the separation of human hemoglobin variants. (elsevier.com)
  • On isoelectric focusing in immobilized pH gradients (IPG) a preparation of recombinant hirudin from Hirudinaria manillensis, purified to homogeneity, was found to still contain a total of 5% minor components: three with higher pI values (pIs 4.10, 4.25 and 4.31), one with a lower pI value (pI 3.98) as compared with the main form (pI 4.03). (univr.it)
  • It has now been over a decade since isoelectric focusing became estab- lished as a valuable addition to our arsenal of physical methods for separa- tion of proteins and other amphoteric substances. (powells.com)
  • Isoelectric Focusing (IEF) can be described as an ingenious process for simultaneous concentration and separation of proteins. (serva.de)
  • Isoelectric focusing in urea using small density gradient columns for dissociated poliovirus resulted in a complete separation of the four virus polypeptides. (microbiologyresearch.org)
  • The isoelectric properties of serum and urinary albumin from normal subjects and patients with nephrotic syndrome have been investigated in various conditions of denaturation, obtained by using urea (0-8 M) as a support in isoelectric focusing. (elsevier.com)
  • Investigation on minor degraded derivatives of the recombinant hirudin variant HM2 from Hirudinaria Manillensis isolated by isoelectric focusing in multicompartment electrolyzers. (univr.it)
  • Multicompartment electrolyzers with isoelectric membranes and micropreparative IPG gel slabs allowed the recovery of pure fractions of such minor components, which were further characterized by electrospray mass spectra, limited proteolysis, and sequence analysis. (univr.it)
  • Isoelectric focusing (IEF), also known as electrofocusing, is a technique for separating different molecules by differences in their isoelectric point (pI). (wikipedia.org)
  • Conse- quently, we considered it desirable to review some of the important appli- cations of the isoelectric focusing technique. (powells.com)
  • This was confirmed by measuring, by the direct-titration technique, the difference in pH of deoxy and fully liganded haemoglobin A 0 (α 2 β 2 ) solutions in conditions approximating those of the isoelectric focusing, i.e. at 5°C and very low concentration of KCl. (biochemj.org)
  • Isoelectric Focusing (IEF) is a technique which is used to separate ampholytic components, such as proteins, based on their isoelectric point (pI). (bio-protocol.org)
  • Here we provide a detailed protocol for determining phosphorylated rhodopsin species using the Isoelectric Focusing technique. (bio-protocol.org)
  • An isoelectric-focusing technique followed by Western blot (immunoblot) analysis was used to investigate the immunoglobulin G response of tuberculous patients against each of the three components of the Mycobacterium bovis BCG antigen 85 complex. (asm.org)
  • Proteins can thus be separated according to their isoelectric point (overall charge) on a polyacrylamide gel using a technique called isoelectric focusing , which uses a pH gradient to separate proteins. (princeton.edu)
  • An isoelectric focusing (IEF) technique using direct peroxidase or peroxidase-antiperoxidase (PAP) overlay staining for detection of cerebrospinal fluid (CSF) oligoclonal IgG is described. (elsevier.com)
  • Orosomucoid (ORM) typing by print lectinofixation: a new technique for isoelectric focusing. (elsevier.com)
  • T2 - a new technique for isoelectric focusing. (elsevier.com)
  • IEF is an end point method - when the electrophoretic run is completed proteins will appear as separate, sharp zones, in the order of their isoelectric points. (serva.de)
  • Bier, M. / Recycling electrophoretic separations : Modeling of isotachophoresis and isoelectric focusing . (elsevier.com)
  • As a particle moves toward the pole opposite of its charge it moves through the changing pH gradient until it reaches a point in which the pH of that molecules isoelectric point is reached. (wikipedia.org)
  • The migration behavior of ions from the hydrogels into the capillary and the formation of a pH gradient in isoelectric focusing using the reagent-release hydrogels were investigated, which confirmed that the prepared reagent-release hydrogels could form a similar pH gradient to that obtained with conventional capillary isoelectric focusing using the solutions. (go.jp)
  • IEF employs a pH gradient formed by small amphoteric molecules (called ampholytes) to resolve proteins according to their different pI values (pI = isoelectric point). (serva.de)
  • The focusing is suggested to take place over a centrally symmetric pH gradient which in the cytosol of the yeast Saccharomyces cerevisiae is known to be 7.2-6.4. (flegr.com)
  • It is obvious that if both an electric field and a pH gradient exist in the interior of the cell, then cytosolic ampholytes (e.g. proteins) are sorted according to their isoelectric points (pI) to occupy stable positions in distinct regions within the cell. (flegr.com)
  • If such a gradient were to be utilised for isoelectric focusing of cell components, a centrally symmetric electric field with a negative potential in the cell centre would have to exist. (flegr.com)
  • Sperm-lysates from 264 unrelated Japanese males were tested for the polymorphism of the human diaphorase DIA 3 by isoelectric focusing in thin-layer polyacrylamide gel over pH range 3.5-9.5 gradient. (nature.com)
  • Indeed, much progress has been made [3] [4] in sample application with the use of immobilized pH gradient strips (IPG) for isoelectric focusing (IEF). (scirp.org)
  • This report describes the fabrication and characterization of a simple and disposable capillary isoelectric focusing ( cIEF ) device containing a reagent-release capillary (RRC) array and poly(dimethylsiloxane) (PDMS) platform, which allows rapid (within 10 min) screening of cIEF conditions by introducing a sample solution into plural RRCs by capillary action followed by electric field application. (rsc.org)
  • A reproducible and automatable method for coupling capillary isoelectric focusing (cIEF) and matrix assisted laser desorption/ionization mass spectrometry (MALDI-MS) based on syringe pump mobilization is found. (asu.edu)
  • Fundamental studies of MALDI-MS on novel superhydrophobic substrates yield unique insights towards an optimal interface between cIEF and MALDI-MS. Finally, the fundamentals of isoelectric focusing in an open drop are explored. (asu.edu)
  • Capillary isoelectric focusing (cIEF) was online coupled to a Q-TOF MS by a flow-through microvial interface for the analysis of therapeutic mAb. (orphiz.be)
  • Nicholas Catsimpoolas James W. Drysdale vii Contents Chapter J Isoelectric Focusing of Human Saliva, Cerebrospinal Fluid, and Urine Josie A. Beeley I. Introduction I II. (powells.com)
  • 5. The detection system of claim 1 further comprising a means for focusing light. (google.ca)
  • 6. The detection system of claim 5 wherein the focusing means is a lens for focusing fluorescent light emitted from the target species in the capillaries to the means for detecting fluorescent light. (google.ca)
  • Narrow-range peptide isoelectric focusing as peptide prefractionation method prior to tandem mass spectrometry analysis. (scilifelab.se)
  • The rationale behind using narrow-range peptide isoelectric focusing as a prefractionation method prior to ms/ms is to reduce the complexity induced by tryptic digestion. (scilifelab.se)
  • By modification of the isoelectric point (pI) of molecules of an enzyme by, e.g., phosphorylation or dephosphorylation, the cell can transfer molecules of the enzyme between different parts of its interior, to switch on or switch off particular biochemical processes. (wikipedia.org)
  • Analysis of four therapeutic monoclonal antibodies by online capillary isoelectric focusing directly coupled to quadrupole time-of-flight mass spectrometry. (orphiz.be)
  • Reagent-release hydrogels containing an acid or a base solution were prepared and employed as ion suppliers in isoelectric focusing using short capillaries. (go.jp)
  • To develop a rapid and sensitive assay based on microfluidic isoelectric focusing, we previously prepared reagent-release capillaries retaining ampholytes by physical adsorption on the inner surface of a short capillary, which were then applied to isoelectric focusing. (go.jp)
  • In this study, reagent-release hydrogels containing an acid or a base solution were developed for isoelectric focusing using short capillaries in place of the acid and base solutions used in conventional microfluidic isoelectric focusing. (go.jp)
  • The prepared reagent-release hydrogels showed some advantages for isoelectric focusing, e.g. , high durability for at least five weeks of storage, easy handling as compared to the conventional solutions, and suppression of hydrodynamic flow in capillaries, which often decreases the reproducibility in capillary isoelectric focusing. (go.jp)
  • Egg production significantly increased only in a fraction with an isoelectric point of pH 5. (bioone.org)
  • In this paper, the possibility of intracellular isoelectric focusing operating in the S. cerevisiae cell is considered from an energetic point of view. (flegr.com)
  • Isoelectric Point" is a descriptor in the National Library of Medicine's controlled vocabulary thesaurus, MeSH (Medical Subject Headings) . (harvard.edu)
  • This graph shows the total number of publications written about "Isoelectric Point" by people in Harvard Catalyst Profiles by year, and whether "Isoelectric Point" was a major or minor topic of these publication. (harvard.edu)
  • Below are the most recent publications written about "Isoelectric Point" by people in Profiles. (harvard.edu)
  • In 2017, researchers stated "The work of P.G. Righetti has been paramount in the ability to separate biomolecules electrophoretically, particularly according to isoelectric point. (wikipedia.org)
  • The isoelectric point ( pI ), sometimes abbreviated to IEP , is the pH at which a particular molecule or surface carries no net electrical charge . (princeton.edu)
  • Conversely, the enzymes specified by the R factors RTEM, R1 and RGN14, with identical isoelectric focusing patterns have the same biochemical properties. (nih.gov)
  • R factor-mediated enzymes gave identical isoelectric focusing patterns irrespective of the host strain. (nih.gov)
  • A model of intracellular electrical sorting of enzymes and organelles in the cytosol, based on isoelectric focusing, is proposed. (flegr.com)
  • Analysis of human corneal IgG by isoelectric focusing. (arvojournals.org)
  • We have used isoelectric focusing to measure the differences between the pI values of various normal and mutant human haemoglobins when completely deoxygenated and when fully liganded with CO. It was assumed that the ΔpI(deox. (biochemj.org)
  • Analysis of human serum proteins by liquid phase isoelectric focusing and matrix-assisted laser desorption/ionization-mass spectrometry. (duke.edu)
  • We used robotized capillary isoelectric focusing as well as conventional immunoblotting for the comprehensive analysis of epidermal growth factor receptor signaling pathways converging on extracellular regulated kinase 1/2 (ERK1/2), AKT, phospholipase Cγ1 (PLCγ1) and c-SRC in normal mucosa compared with CRC stage II and IV. (biomedcentral.com)
  • Abnormal CSF immunoglobulin components detected by isoelectric focusing. (naver.com)
  • The use of both HPLC retention time and isoelectric focusing position provides an accurate presumed diagnosis of a rare hemoglobin variant in the majority of cases. (bvsalud.org)
  • Isoelectric focusing can resolve proteins that differ in pI value by as little as 0.01. (wikipedia.org)
  • Serna-Sanz A., Rairdan G., Peck S.C. (2007) Preparative Denaturing Isoelectric Focusing for Enhancing Sensitivity of Proteomic Studies. (springer.com)
  • Immunoblotting of the different signal transducers, run in parallel to capillary isoelectric focusing, showed higher variability and lower sensitivity and resolution. (biomedcentral.com)
  • was similar to the isoelectric-focusing curve relating the variation of ΔpI(deox. (biochemj.org)
  • To determine the isoelectric points of unknown proteins the pH of focused bands may be measured on the gel using a surface electrode. (serva.de)
  • The isoelectric points ranged from 3·9 to 8·7 and were not related to the substrate profiles or other biochemical properties. (microbiologyresearch.org)
  • The results showed that the strength of a centrally symmetric intracytosolic electric field could be as high as 90 mV/cm, which is sufficient to account for sorting of cytosolic proteins according to their isoelectric points. (flegr.com)
  • By separating radiolabeled EPS via isoelectric focusing, we found that isoelectric points are radionuclide specific, suggesting that each radionuclide binds to specific biopolymeric functional groups, with the most efficient binding sites likely occurring in acid polysaccharides, iron hydroxides, and proteins. (bco-dmo.org)
  • Petersen, John R. / Clinical application of capillary isoelectric focusing on fused silica capillary for determination of hemoglobin variants . (utmb.edu)
  • IPG strips are placed into a focusing tray, covered with mineral oil, and the tray is placed into the IEF cell for 5-6 hrs. (psu.edu)