OrganismsChemicals and DrugsPhenomena and ProcessesAnthropology, Education, Sociology and Social PhenomenaHealth Care
LeuconostocLactic AcidLactobacillusUnited States Food and Drug AdministrationLactobacillaceaePediococcusFermentation
Leuconostoc
A genus of gram-positive, facultatively anaerobic bacteria whose growth is dependent on the presence of a fermentable carbohydrate. It is nonpathogenic to plants and animals, including humans.
Lactic Acid
A normal intermediate in the fermentation (oxidation, metabolism) of sugar. The concentrated form is used internally to prevent gastrointestinal fermentation. (From Stedman, 26th ed)
Lactobacillus
A genus of gram-positive, microaerophilic, rod-shaped bacteria occurring widely in nature. Its species are also part of the many normal flora of the mouth, intestinal tract, and vagina of many mammals, including humans. Pathogenicity from this genus is rare.
United States Food and Drug Administration
An agency of the PUBLIC HEALTH SERVICE concerned with the overall planning, promoting, and administering of programs pertaining to maintaining standards of quality of foods, drugs, therapeutic devices, etc.
Lactobacillaceae
A family of gram-positive bacteria found regularly in the mouth and intestinal tract of man and other animals, in food and dairy products, and in fermenting vegetable juices. A few species are highly pathogenic.
Pediococcus
A genus of gram-positive, facultatively anaerobic bacteria whose growth is dependent on the presence of a fermentable carbohydrate. No endospores are produced. Its organisms are found in fermenting plant products and are nonpathogenic to plants and animals, including humans.
Fermentation
Anaerobic degradation of GLUCOSE or other organic nutrients to gain energy in the form of ATP. End products vary depending on organisms, substrates, and enzymatic pathways. Common fermentation products include ETHANOL and LACTIC ACID.

Differential expression and phosphorylation of CTCF, a c-myc transcriptional regulator, during differentiation of human myeloid cells. (1/3619)

CTCF is a transcriptional repressor of the c-myc gene. Although CTCF has been characterized in some detail, there is very little information about the regulation of CTCF activity. Therefore we investigated CTCF expression and phosphorylation during induced differentiation of human myeloid leukemia cells. We found that: (i) both CTCF mRNA and protein are down-regulated during terminal differentiation in most cell lines tested; (ii) CTCF down-regulation is retarded and less pronounced than that of c-myc; (iii) CTCF protein is differentially phosphorylated and the phosphorylation profiles depend on the differentiation pathway. We concluded that CTCF expression and activity is controlled at transcriptional and post-transcriptional levels.  (+info)

Human triclonal anti-IgG gammopathy. I. Iso-electric focusing characteristics of the IgG, IgA and IgM anti-IgG and their heavy and light chains. (2/3619)

Human IgG, IgA and IgM anti-IgG autoantibodies have been isolated from the serum of an individual with Felty's syndrome. These were initially noted as soluble circulating serum complexes by analytical ultracentrifugation. Isolation was accomplished by solid phase immunoadsorption and each of the three antibody populations obtained was shown to be of restricted heterogeneity by liquid and polyacrylamide gel electrofocussing methods. Type kappa light chains were obtained from each protein. Co-isoelectric focusing experiments of all possible pairs of these light chains showed them to have identical net charge characteristics. Heavy chains obtained from each protein were also monoclonal and of differing isoelectric point. The availability of this serum provides a human model with which to study the changes which may occur in autoantibodies during the autoimmune response.  (+info)

Molecular and biochemical characterization of VEB-1, a novel class A extended-spectrum beta-lactamase encoded by an Escherichia coli integron gene. (3/3619)

A clinical isolate, Escherichia coli MG-1, isolated from a 4-month-old Vietnamese orphan child, produced a beta-lactamase conferring resistance to extended-spectrum cephalosporins and aztreonam. In a disk diffusion test, a typical synergistic effect between ceftazidime or aztreonam and clavulanic acid was observed along with an unusual synergy between cefoxitin and cefuroxime. The gene for VEB-1 (Vietnamese extended-spectrum beta-lactamase) was cloned and expressed in E. coli JM109. The recombinant plasmid pRLT1 produced a beta-lactamase with a pI of 5.35 and conferred high-level resistance to extended-spectrum (or oxyimino) cephalosporins and to aztreonam. Vmax values for extended-spectrum cephalosporins were uncommonly high, while the affinity of the enzyme for ceftazidime and aztreonam was relatively low. blaVEB-1 showed significant homology at the DNA level with only blaPER-1 and blaPER-2. Analysis of the deduced protein sequence showed that VEB-1 is a class A penicillinase having very low levels of homology with any other known beta-lactamases. The highest percentage of amino acid identity was 38% with PER-1 or PER-2, two uncommon class A extended-spectrum enzymes. Exploration of the genetic environment of blaVEB-1 revealed the presence of gene cassette features, i.e., (i) a 59-base element associated with blaVEB-1; (ii) a second 59-base element just upstream of blaVEB-1, likely belonging to the aacA1-orfG gene cassette; (iii) two core sites (GTTRRRY) on both sides of blaVEB-1; and (iv) a second antibiotic resistance gene 3' of blaVEB-1, aadB. blaVEB-1 may therefore be the first class A extended-spectrum beta-lactamase that is part of a gene cassette, which itself is likely to be located on a class 1 integron, as sulfamide resistance may indicate. Furthermore, blaVEB-1 is encoded on a large (> 100-kb) transferable plasmid found in a Klebsiella pneumoniae MG-2 isolated at the same time from the same patient, indicating a horizontal gene transfer.  (+info)

Mechanisms of beta-lactam resistance amongst Pseudomonas aeruginosa isolated in an Italian survey. (4/3619)

The mechanisms of resistance to beta-lactam antibiotics in 325 isolates of Pseudomonas aeruginosa were examined. These isolates were selected because of their resistance to meropenem and imipenem (breakpoint, >4 mg/L), carbenicillin (>128 mg/L), ceftazidime (>8 mg/L), piperacillin and ticarcillin/clavulanate (>64 mg/L). The most frequent mechanism of resistance was beta-lactamase-independent, so called 'intrinsic resistance', which was found in 183 isolates and was probably due to impermeability and/or efflux mechanisms. beta-Lactamase-mediated resistance was demonstrated in 111 strains (11.1%). Derepression of Ambler Class C chromosomal beta-lactamase was detected in 64 isolates, most of which were resistant to ceftazidime and piperacillin but susceptible to meropenem, whereas secondary plasmid-encoded beta-lactamases were found in 34 isolates, all of them resistant to carboxypenicillins and ureidopenicillins and susceptible to carbapenems. Twelve strains showed more than one plasmid-encoded beta-lactamase plus derepression of chromosomal Class C enzyme. Resistance to carbapenems was independent of resistance to other beta-lactam antibiotics, indicating a different mechanism of resistance, probably due to the loss of the D2 porin. In total, 32 strains were resistant to carbapenems: 24 only to imipenem and eight to both imipenem and meropenem.  (+info)

Differential serodiagnosis for cystic and alveolar echinococcosis using fractions of Echinococcus granulosus cyst fluid (antigen B) and E. multilocularis protoscolex (EM18). (5/3619)

Echinococcus granulosus cyst fluid and E. multilocularis protoscolex extract were fractionated by a single step of preparative isoelectric focusing, resulting in an antigen B-rich fraction (8-kD) and an Em18-rich fraction, respectively. The usefulness of both fractions for differential serodiagnosis of cystic (CE) and alveolar (AE) echinococcosis was evaluated by a large-scale immunoblot analysis on a battery of 354 serum samples. These included 66 from AE patients originating from four different endemic areas, 173 from CE patients originating from seven different endemic areas, 71 from patients with other parasitic diseases, 15 from patients with hepatomas, and 29 from healthy individuals. In an immunoblot with the antigen B-rich fraction, 92% (158 of 173) of the CE sera as well as 79% (52 of 66) of the AE sera reacted with the 8-kD subunit. No cross-reactivity occurred with any sera from patients with cysticercosis, other parasitic diseases, or with hepatomas, or from healthy controls. In an immunoblot with the Em18-rich fraction, all but two sera from AE patients (64 of 66, 97%) recognized Em18, and only nine of 34 CE sera from China reacted with it. All other (139) CE sera from six other countries were negative as were all (115) other non-echinococcosis sera. These findings indicate that antigen B (8-kD) is not species-specific for E. granulosus but is genus-specific for Echinococcus, and that the Em18 antigen is a reliable serologic marker for species-specific differentiation of AE from CE.  (+info)

The relationship of glycosylation and isoelectric point with tumor accumulation of avidin. (6/3619)

Radiolabeled avidin markedly accumulated in intraperitoneal tumors and was cleared rapidly from circulation when given intraperitoneally. This study investigated the mechanisms of the tumor localization of avidin. METHODS: Avidin was deglycosylated through endoglycosydase-H digestion and/or neutralized by acetylation of its lysine amino acids with acetic acid N-hydroxysuccinimide ester. Avidin and modified avidins were analyzed using sodium dodecylsulfate-polyacrylamide gel electrophoresis (SDS/PAGE) and isoelectric focusing. A tumor model was established by intraperitoneal injection of human colon cancer cells, LS180, in nude mice. Avidin and modified avidins were labeled with 111In using diethyleneamine pentaacetic acid-biotin and were administered intraperitoneally into the tumor-bearing mice. The biodistribution of radioactivity was examined 2 and 24 h postinjection. RESULTS: Deglycosylated avidins revealed a major band of smaller molecules on SDS/PAGE. The isoelectric point of neutralized avidins was reduced to less than 5, whereas that of unneutralized avidins was more than 9.5. Biodistribution study demonstrated that liver uptake was decreased by deglycosylation and kidney accumulation was decreased by neutralization, respectively. The blood clearance was remarkably slowed by combined modification of deglycosylation and neutralization. The tumor uptake of radioactivity was reduced by either deglycosylation or neutralization and was further decreased with combined modification. CONCLUSION: Both high glycosylation and positive charge of avidin contributed to its accumulation in tumor. This study may facilitate development of a new vehicle for the delivery of therapeutic agents to intraperitoneal tumors.  (+info)

Isolation of pigment-binding early light-inducible proteins from pea. (7/3619)

The early light-inducible proteins (ELIPs) in chloroplasts possess a high sequence homology with the chlorophyll a/b-binding proteins but differ from those proteins by their substoichiometric and transient appearance. In the present study ELIPs of pea were isolated by a two-step purification strategy: perfusion chromatography in combination with preparative isoelectric focussing. Two heterogeneous populations of ELIPs were obtained after chromatographic separation of solubilized thylakoid membranes using a weak anion exchange column. One of these populations contained ELIPs in a free form providing the first isolation of these proteins. To prove whether the isolated and pure forms of ELIP bind pigments, spectroscopic and chromatographic analysis were performed. Absorption spectra and TLC revealed the presence of chlorophyll a and lutein. Measurements of steady-state fluorescence emission spectra at 77 K exhibited a major peak at 674 nm typical for chlorophyll a bound to the protein matrix. The action spectrum of the fluorescence emission measured at 674 nm showed several peaks originating mainly from chlorophyll a. It is proposed that ELIPs are transient chlorophyll-binding proteins not involved in light-harvesting but functioning as scavengers for chlorophyll molecules during turnover of pigment-binding proteins.  (+info)

Low-temperature sensitivity and enhanced Bohr effect in red deer (Cervus elaphus) haemoglobin: a molecular adaptive strategy to life at high altitude and low temperature. (8/3619)

A study of the functional properties of haemoglobin from red deer (Cervus elaphus) whose habitat varies over a wide range of latitude, was performed. The oxygen-binding properties of the most common haemoglobin phenotype from the species living in Sardinia were examined with particular attention to the effect of pH, chloride, 2, 3-bisphosphoglycerate and temperature. Results indicate that red deer haemoglobin, like all haemoglobins from ruminants so far examined, is characterized by a low intrinsic oxygen affinity, with chloride being its main physiological modulator in vivo. The functional results and the low temperature sensitivity of the oxygen affinity are discussed in the light of the amino acid sequence of closely related ruminant haemoglobins.  (+info)

Plus itPlus it
The nuclear proteins of regenerating and fetal rat liver, slow-growing Morris hepatoma 9618A, and fast-growing Morris hepatoma 3924A, sequentially extracted from nuclei with (a) 0.075 m NaCl/0.025 m EDTA, (b) 10 mm Tris, (c) 0.35 m NaCl, (d) 0.6 m NaCl, and (e) 3 m NaCl/7 m urea, were analyzed by two-dimensional, isoelectric-focusing sodium dodecyl sulfate gel electrophoresis. Many of the protein spots were common to these tissues as well as to the Novikoff hepatoma and normal liver. The protein patterns of the regenerating liver and slow-growing 9618A hepatoma were more similar to that of normal liver than to those of other tissues. Many similarities were found between the fetal liver and the fast-growing 3924A and Novikoff hepatomas. Four protein spots, 64/5.9, 60/6.3, 51/5.3, and 38/7.3 (M.W. × 10-3/pl), were only found in the fast-growing 3924A and Novikoff hepatomas. Proteins 79/6.4 and 61/7.2 were only found in the three hepatomas. Protein 37/6.3 was dense in the three hepatomas and much ...
http://cancerres.aacrjournals.org/content/39/6_Part_1/2096
Buffer electrofocusing of Interleukin I | Bioscience Reports | Portland PressBuffer electrofocusing of Interleukin I | Bioscience Reports | Portland Press
R. L. Prestidge, W. J. Koopman, J. C. Bennett, M. T. W. Hearn; Buffer electrofocusing of Interleukin I. Biosci Rep 1 April 1982; 2 (4): 241-246. doi: https://doi.org/10.1007/BF01136723. Download citation file:. ...
https://portlandpress.com/bioscirep/article-abstract/2/4/241/54277/Buffer-electrofocusing-of-Interleukin-I?redirectedFrom=fulltext
Genovis » Blog » Charge Heterogeneity Analysis of Antibody Subunits Generated by FragITGenovis » Blog » Charge Heterogeneity Analysis of Antibody Subunits Generated by FragIT
Charge heterogeneity of monoclonal antibodies is an important critical quality attribute that requires close monitoring due to its potential impact on antibody efficacy and immunogenicity. Since the heterogeneity is mostly caused by post translational modifications such as C-terminal lysine clipping, deamidation, glycation, sialic acid or adduct formation, these modifications can pose significant challenges to the analytical scientists.. In a recently published article by Jaag et al., scientists from the Institute of Pharmaceutical Sciences at University at Tübingen, Germany, present a new approach to charge variant analysis at the intact and sub-unit level by 2D-LC separation. In this workflow, the first-dimension separation is based on strong-cation chromatography (SCX) and the second-dimension separation is based on desalting reversed phase liquid chromatography (RP-HPLC) which enables combination with the mass analysis by mass spectrometric (MS) detection.. The analysis at the subunit level ...
https://www.genovis.com/blog/charge-heterogeneity-analysis-of-antibody-subunits-generated-by-fragit/
An investigation of the surface properties of atomized Fe<sub>x</sub>Si...
TY - JOUR. T1 - An investigation of the surface properties of atomized FexSi powders. AU - Williams, Richard Andrew. AU - Kelsall, Geoffrey Howard. PY - 1989/10/1. Y1 - 1989/10/1. N2 - The results of XPS and electrokinetic measurements on spherical ferrosilicide particles (15 wt% Si) produced by atomization show that the surface consists of a mixed silica and Fe(II, III) oxide layer of at least 50 nm thickness. Although the surface is dominated by the silica phase (up to 70 at %), the electrophoretic mobility response in aqueous KCl electrolytes is complex and exhibits three points of charge reversal over the pH range 3-11. These phenomena correspond to an apparent point of zero charge of the surface at about pH 5 (which varies with surface composition), a point of charge reversal (which is independent of surface composition) at pH 7 due to the adsorption of FeOH+ ions originating from the substrate, and an isoelectric point at pH 7.6 due to transformation of an essentially siliceous surface to ...
https://researchportal.hw.ac.uk/en/publications/an-investigation-of-the-surface-properties-of-atomized-fesubxsubs
New report shares details about the United States plasminogen market - WhaTechNew report shares details about the United States plasminogen market - WhaTech
Plasminogen, the proenzyme of the fibrinolytically active enzyme plasmin, is a single chain glycoprotein with a molecular weight of about 90,000-94,000 Da (2). Various isoelectric forms exist and can be separated by means of isoelectric focusing (IEF).
https://www.whatech.com/market-research/industrial/372749-new-report-shares-details-about-the-united-states-plasminogen-market
Products: X-ray poly capillary optics with a conically shaped basic form | SAMRAT MACHINE & TOOLSProducts: X-ray poly capillary optics with a conically shaped basic form | SAMRAT MACHINE & TOOLS
A Poly-CCC (Poly Capillary Conic Collimator) is an X-ray poly capillary optics with a conically shaped basic form. Divergent X-rays are collimated and guided.
https://www.helmut-fischer.com/en/egypt/products/high-precision-capillary-optics-for-beam-shaping-of-x-rays/x-ray-optics-to-guide-and-shape-x-radiation/x-ray-poly-capillary-optics-with-a-conically-shaped-basic-form/
Clonal nature of the immune response. II. The effect of immunization of clonal commitmentClonal nature of the immune response. II. The effect of immunization of clonal commitment
An inbred strain can produce several hundred different anti-group A carbohydrate (GAC) antibodies, as analyzed by isoelectric focusing. However, each individual mouse produces the bulk of its anti-GAC antibody as only one or two different spectrotypes, which appear to be randomly chosen. By using adoptive transfer techniques, we have observed that clonal commitment occurs very early in immunization, sometimes even before immunization, and thus does not result from competition among B cells for antigen ...
https://scholars.uab.edu/display/pub764511
Products in Reagents, P, GE Healthcare on Thomas ScientificProducts in Reagents, P, GE Healthcare on Thomas Scientific
* found in: Pharmalyte, Pharmalyte, narrow range pH 4.2-4.9 Pharmalyte, narrow range pH 4.2-4.9 Pharmalyte carrier ampholytes, prepared by the co..
http://www.thomassci.com/nav/cat1/reagentsp/manufacturer/gehealthcare/0
Indexes: Dynamic Fields | RavenDB 4.1 DocumentationIndexes: Dynamic Fields | RavenDB 4.1 Documentation
While strongly typed entities are well processed by LINQ expressions, some scenarios demand the use of dynamic properties. To support searching in object gra...
https://ravendb.net/docs/article-page/4.1/java/indexes/using-dynamic-fields
Oxford Reference - Answers with AuthorityOxford Reference - Answers with Authority
The study of US foreign relations is one of the most dynamic fields of American history. The availability of new sources in recent years has opened new opportunities for examining US behavior through the lenses of other nations. Meanwhile, historians of international affairs have increasingly borrowed the methods, questions, and insights of cultural and social history, enlivening their field and opening bold new lines of interpretation... Read more ,. ...
https://www.oxfordreference.com/
Articles | PIH
This summer, follow these six savvy rules of healthy eating: Eat Whole Foods, not processed Any food in its most essential, pure and basic form is nutrient dense and must …. ...
http://wheybetterguard.com/category/articles/
News | PIH
This summer, follow these six savvy rules of healthy eating: Eat Whole Foods, not processed Any food in its most essential, pure and basic form is nutrient dense and must …. ...
http://wheybetterguard.com/news/page/3/
How effective is Underfloor Heating?How effective is Underfloor Heating?
How effective is Underfloor Heating? | ArticlesList.net. Underfloor heating has been around far longer than most people realise. The Romans used a basic form of
http://www.articleslist.net/home-family.asp?how-effective-is-underfloor-heating=0&c=2&articleid=21993
Membranes | Free Full-Text | Nanofiltration of Mine Water: Impact of Feed pH and Membrane Charge on Resource Recovery and Water...Membranes | Free Full-Text | Nanofiltration of Mine Water: Impact of Feed pH and Membrane Charge on Resource Recovery and Water...
Two nanofiltration membranes, a Dow NF 270 polyamide thin film and a TriSep TS 80 polyamide thin film, were investigated for their retention of ionic species when filtering mine influenced water streams at a range of acidic pH values. The functional iso-electric point of the membranes, characterized by changes in retention over a small pH range, were examined by filtering solutions of sodium sulphate. Both membranes showed changes in retention at pH 3, suggesting a zero net charge on the membranes at this pH. Copper mine drainage and synthetic solutions of mine influenced water were filtered using the same membranes. These solutions were characterized by pH values within 2 and 5, thus crossing the iso-electric point of both membranes. Retention of cations was maximized when the feed solution pH was less than the iso-electric point of the membrane. In these conditions, the membrane has a net positive charge, reducing the transmission rate of cations. From the recoveries of a range of cations, the
http://www.mdpi.com/2077-0375/4/2/163/xml
Nanofiltration of mine water: Impact of feed pH and membrane charge on resource recovery and water discharge - Murdoch...Nanofiltration of mine water: Impact of feed pH and membrane charge on resource recovery and water discharge - Murdoch...
Two nanofiltration membranes, a Dow NF 270 polyamide thin film and a TriSep TS 80 polyamide thin film, were investigated for their retention of ionic species when filtering mine influenced water streams at a range of acidic pH values. The functional iso-electric point of the membranes, characterized by changes in retention over a small pH range, were examined by filtering solutions of sodium sulphate. Both membranes showed changes in retention at pH 3, suggesting a zero net charge on the membranes at this pH. Copper mine drainage and synthetic solutions of mine influenced water were filtered using the same membranes. These solutions were characterized by pH values within 2 and 5, thus crossing the iso-electric point of both membranes. Retention of cations was maximized when the feed solution pH was less than the iso-electric point of the membrane. In these conditions, the membrane has a net positive charge, reducing the transmission rate of cations. From the recoveries of a range of cations, the ...
http://researchrepository.murdoch.edu.au/id/eprint/21905/
Mary Lou Caspers, Ph.D. at University of Detroit MercyMary Lou Caspers, Ph.D. at University of Detroit Mercy
Brown, R.K., Caspers, M.L., Lull, J.M., Vinogradov, S.N., Felgenhauer, K. and Nekic, M. (1977). Carrier Ampholyte Distribution in Isoelectric Focusing. J. Chromatogr. 131, 223-232.. Brown, R.K., Caspers, M.L. and Vinogradov, S.N. (1977). Carrier Ampholyte Distribution. In Electrofocusing and Isotachophoresis (Radola, B.J. and Graesslin, D. Eds.) Walter DeGrutyer and Co., Berlin-New York, pp 87-96.. Caspers, M.L., Posey, Y. and Brown, R.K. (1977). Separator Isoelectric Focusing - An Improved Method of Protein Analysis and Purification. Anal. Biochem. 79, 166-180.. Caspers, M.L. and Chrambach, A. (1977). Natural pH Gradients Formed by Amino Acids: Ampholyte Distribution, Time Course, Use in Electrofocusing of Protein, Relation to pH Gradients in Isotachophoresis, Separator Effects. Anal. Biochem. 81, 23-39.. Caspers, M.L. and Siegel, G.J. (1980). Inhibition by Lead of Human Erythrocyte (Na+ + K+)-Adenosine Triphosphatase Associated with Binding of 210Pb to Membrane Fragments. Biochim. et. Biophy. ...
http://www.udmercy.edu/about/people/university/ces/chemistry/caspers-mary-lou.php
Isoelectric Focusing›Electrophoresis › SERVA Electrophoresis GmbHIsoelectric Focusing›Electrophoresis › SERVA Electrophoresis GmbH
Isoelectric Focusing (IEF) can be described as an ingenious process for simultaneous concentration and separation of proteins. IEF employs a pH gradient formed by small amphoteric molecules (called ampholytes) to resolve proteins according to their different pI values (pI = isoelectric point). IEF is an end point method - when the electrophoretic run is completed proteins will appear as separate, sharp zones, in the order of their isoelectric points.. For IEF, SERVA offers a comprehensive product line: ...
https://www.serva.de/enDE/Catalog/186_Electrophoresis_Isoelectric_Focusing.html
RUL - Flow-induced surface charge heterogeneity in electrokinetics due to Stern-layer conductance coupled to reaction kineticsRUL - Flow-induced surface charge heterogeneity in electrokinetics due to Stern-layer conductance coupled to reaction kinetics
We theoretically study the electrokinetic problem of a pressure-induced liquid flow through a narrow long channel with charged walls, going beyond the classical Helmholtz-Schmolukowski picture by considering the surprisingly strong combined effect of (i) Stern-layer conductance and (ii) dynamic chargeregulating rather than fixed surface charges. We find that the water flow induces, apart from the well-known streaming potential, also a strongly heterogeneous surface charge and zeta potential on chemically homogeneous channel walls. Moreover, we identify a novel steady state with a nontrivial 3D electric flux with 2D surface charges acting as sources and sinks. For a pulsed pressure drop our findings also provide a first-principles explanation for ill-understood experiments on the effect of flow on interfacial chemistry [D. Lis et al., Science 344, 1138 (2014 ...
https://repozitorij.uni-lj.si/IzpisGradiva.php?id=101750&lang=eng
pI and pH relationship in context of ion exchange protein purification - Biology Stack ExchangepI and pH relationship in context of ion exchange protein purification - Biology Stack Exchange
The pI (iso-electric point) is the pH at which the protein (or other molecule), overall has a net zero charge. As @Chris points out, the buffer you are using will change the pH the protein finds itself in. This will change the charge of the protein. In ion exchange chromatography, there are charged chemical groups on the resin and these can cause a protein to be retained on the resin if the protein is complementary (opposite charged) to the resin.. So if you have a protein with a pI of 5 say, it would neutral with the presence of excess H+. This implies that the protein has an overall negative charge. A cationic resin would tend to retain this protein in a pH 7 buffer. I hope that makes sense. In most cases, ion exchange chromatography is used when most of the protein is separated out and there are a few proteins (maybe a couple of hundred) mixed together. It can get tricky if you see that the protein is mixed in with something that is also negatively charged. In that case you might change the ...
https://biology.stackexchange.com/questions/15913/pi-and-ph-relationship-in-context-of-ion-exchange-protein-purification
Product listProduct list
Browse our electrophoresis products: carrier ampholytes, electrophoresis chamber, power supply, acrylamide gel support sheets and seed purity testing kits.
http://www.protec-biosep.de/isoelectric-focusing/product-list/
Browse In Neurosurgical Focus | jnsBrowse In Neurosurgical Focus | jns
Two hundred twenty-two patients (168 women, 54 men) were included. The mean age was 52.2 ± 19.3 years (range 18-84 years). The mean follow-up was 28.8 ± 8.2 months (range 24-62 months). There was a significant correlation between PI − LL and PI (r = 0.441, p , 0.001), threatening the use of PI − LL to quantify spinopelvic mismatch for different PI values. RLL was not correlated with PI (r = −0.093, p , 0.05); therefore, it was able to quantify divergence from ideal lordosis for all PI values. Compared with PI − LL, RLL had stronger correlations with HRQOL scores (p , 0.05). Discrimination performance was better for the model with RLL than for PI − LL. The agreement between RLL and PI − LL was high (κ = 0.943, p , 0.001), moderate (κ = 0.455, p , 0.001), and poor (κ = −0.154, p = 0.343), respectively, for large, average, and small PI sizes. When analyzed by RLL, each PI − LL category was further divided into distinct groups of patients who had different mechanical ...
https://thejns.org/browse?access=user&authf=S%C3%A1nchez+P%C3%A9rez-Grueso%2C+Francisco+Javier&authf_0=Sogunmez%2C+Nuray&authf_1=Boissi%C3%A9re%2C+Louis&pageSize=10&sort=datedescending&t=foc
Browse In Neurosurgical Focus | jnsBrowse In Neurosurgical Focus | jns
Two hundred twenty-two patients (168 women, 54 men) were included. The mean age was 52.2 ± 19.3 years (range 18-84 years). The mean follow-up was 28.8 ± 8.2 months (range 24-62 months). There was a significant correlation between PI − LL and PI (r = 0.441, p , 0.001), threatening the use of PI − LL to quantify spinopelvic mismatch for different PI values. RLL was not correlated with PI (r = −0.093, p , 0.05); therefore, it was able to quantify divergence from ideal lordosis for all PI values. Compared with PI − LL, RLL had stronger correlations with HRQOL scores (p , 0.05). Discrimination performance was better for the model with RLL than for PI − LL. The agreement between RLL and PI − LL was high (κ = 0.943, p , 0.001), moderate (κ = 0.455, p , 0.001), and poor (κ = −0.154, p = 0.343), respectively, for large, average, and small PI sizes. When analyzed by RLL, each PI − LL category was further divided into distinct groups of patients who had different mechanical ...
https://thejns.org/browse?authf_0=Sogunmez%2C+Nuray&authf_1=Yilgor%2C+Caglar&pageSize=10&sort=datedescending&t=foc
Learning curves of cardiac surgery in relation to risk stratification and hospital location - The Journal of Cardiovascular...
Results. At PUH 298 -patients -were oper-ated and 312 -patients at PI. -There -were 136 low -risk (EuroS-CORE 0-2) and 474 -medium and -high--risk -patients (EuroS-CORE ≥3). -There was no sig-nif-i-cantly ele-vated mor-tality or -learning -curve in low -risk sur-gery -either at PUH (57 -patients -with 1 -death) or PI (79 -patients 1 -death). In -medium and -high--risk sur-gery at PI -there was no mor-tality in 68 -patients oper-ated by the -senior sur-geon and no -learning -curve in all 233 -such -patients. In 240 -medium and -high--risk -patients at PUH -there was a -learning -curve -despite the involve-ment of the -same -senior sur-geon. In 1999 and 2000 the -observed mor-tality (OM) in 150 -patients was 15.33%, EuroS-CORE 5.98, and in 2001 in 91 -patients OM 3.29%, EuroS-CORE 5.95 -with p=0.0038 -when expe-ri-enced ICU -staff was -employed. LOS was sig-nif-i-cantly -reduced in 97 -patients in 2001 at PUH (8.7 d ± 2.81 vs 11.07-days ± 7.9 in 1999 and 2000, p=0.046) con-firming the ...
https://www.minervamedica.it/it/riviste/cardiovascular-surgery/articolo.php?cod=R37Y2003N05A0591
Betaine HCl Pepsin - Lemon Water Wellness Clinic & General StoreBetaine HCl Pepsin - Lemon Water Wellness Clinic & General Store
• Features an acidic form of betaine to aid digestion • Includes the digestive enzyme pepsin Betaine HCl and pepsin are naturally occurring gastric-juice components that render nutrients available for absorption and biological activity. Betaine HCl is an acidic form of betaine, which promotes optimal gastric lumen acid
https://www.lemonwaterwellness.ca/products/bp2c-c
How to separate proteins of equal size - Protein and ProteomicsHow to separate proteins of equal size - Protein and Proteomics
native page separates based on charge (with size constraints due to limiting pore size of the gel). if you have two proteins of the same size but different pI then you should be able to resolve them on native or urea or ief page ...
http://www.protocol-online.org/biology-forums/posts/36428.html
Biology, Animal Structure and Function, The Animal Body: Basic Form and Function, Homeostasis | OER CommonsBiology, Animal Structure and Function, The Animal Body: Basic Form and Function, Homeostasis | OER Commons
Animal organs and organ systems constantly adjust to internal and external changes through a process called homeostasis (steady state). These changes might be in the level of glucose or calcium in blood or in external temperatures. Homeostasis means to maintain dynamic equilibrium in the body. It is dynamic because it is constantly adjusting to the changes that the bodys systems encounter. It is equilibrium because body functions are kept within specific ranges. Even an animal that is apparently inactive is maintaining this homeostatic equilibrium.. ...
https://www.oercommons.org/courseware/lesson/15108/student/
You searched for - Page 12 of 106 - Alabama Cooperative Extension System
AUBURN UNIVERSITY, Ala. - For farmers in Alabama, fall is one of the busiest times of the year. They are working around the clock to harvest and transport the crops they worked hard to grow. During this time, farmers use many different pi…. ...
https://www.aces.edu/?s=&tags=2639&paged=12
Page Kidney - Rare but Correctable Cause of HypertensionPage Kidney - Rare but Correctable Cause of Hypertension
UroToday - GU OncToday brings coverage of the clinically relevant content needed to stay at the forefront of the dynamic field of GU oncology and urology.
https://www.urotoday.com/volume-4-2011/vol-4-issue-5/48779-page-kidney-rare-but-correctable-cause-of-hypertension.html
Albumin ArticlesAlbumin Articles
UroToday - GU OncToday brings coverage of the clinically relevant content needed to stay at the forefront of the dynamic field of GU oncology and urology.
https://www.urotoday.com/tags/albumin.html
Compute the isoelectric point pi of each of the given amino, Ask an ExpertCompute the isoelectric point pi of each of the given amino, Ask an Expert
compute the isoelectric point pi of each of the given amino acidsa glycineb glutamic acidc, Hire Biology Expert, Ask Academics Expert, Assignment Help, Homework Help, Textbooks Solutions
http://www.mywordsolution.com/question/compute-the-isoelectric-point-pi-of-each-of-the/936583
The Protean i12 System for Isoelectric Focusing | SelectScienceThe Protean i12 System for Isoelectric Focusing | SelectScience
The PROTEAN i12 IEF System, from Bio-Rad Laboratories, is used for isoelectric focusing (IEF) of immobilized pH gradient (IPG) strips. The PROTEAN i12 IEF System can simultaneously run up to 12 IPG strips in 12 independently programmed lanes. Watch this video to learn about more unique features and benefits of this system, which can be used for in-gel peptide fractionation prior to LC-MS.
http://www.selectscience.net/SelectScience-TV/Videos/?pageNumber=7&apptypeID=1,2,3&videoID=1057
Configure individual plastic laser parts online | S-PolytecConfigure individual plastic laser parts online | S-Polytec
Buy plastic sheets as laser cut parts online at low prices. Numerous basic forms ✓ Various configuration possibilities ✓ polished laser edge ✓
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The Case for Animal ESP and Telepathy | HubPagesThe Case for Animal ESP and Telepathy | HubPages
Do animals have a basic form of telepathy? From incredible journeys of cats and dogs, to Jim the Wonder Dog, there are true stories that will make you wonder if the animal world has hidden secrets.
https://hubpages.com/religion-philosophy/The-Case-for-Animal-ESP-and-Telepathy
Why Factoring?Why Factoring?
In its most basic form, factoring is very easy to understand. A company is growing and needs extra capital to make sure that bills are being paid on time. This includes operating bills or payroll and taxes. Factoring accounts receivable is where the finance company uses loans, in the form of invoices, to provide the outside capital companies need to keep growing.
https://ccassociates.com/factoring-e-learning-center/Why-Factoring/
Plus itPlus it
Fucosyltransferase (FT) activity of normal lymphocytes, normal granulocytes, and various types of human leukemic cells and electrofocusing pattern of FT activity in human leukemic cells and normal lymphocytes were examined using asialofetuin as an acceptor. Levels of FT activity in normal lymphocytes were higher than those of normal granulocytes in which FT activity was almost undetectable. The FT activity was higher in blast cells of acute myeloblastic leukemia and chronic myelogenous leukemia in blast crisis than in blast cells of acute lymphoblastic leukemia and the chronic phase of chronic myelogenous leukemia. The level of FT activity was lower in cells of chronic lymphocytic leukemia than that of normal lymphocytes, but it was higher than that of normal granulocytes. Three main isoelectric forms of FT in leukemic blast cells were identified by isoelectrofocusing, and they each had a characteristic focusing point: around pH 4.5 (peak 1); pH 4.9 (peak 2); and pH 5.2 (peak 3). In blast cells ...
http://cancerres.aacrjournals.org/content/47/11/2782
Dogs Cerebrospinal Flu Total Protein Concentrations and IgG Isoelectrofocusing (IEF) Patterns - WSAVA2002 - VINDogs Cerebrospinal Flu Total Protein Concentrations and IgG Isoelectrofocusing (IEF) Patterns - WSAVA2002 - VIN
OBJECTIVES. Isoelectric focusing (IEF) is a laboratory technique that allows to separate proteic molecules based on their isoelectric point. This technique, applied to cerebro spinal fluid (CSF) IgG, has been used to study human neurological diseases. CSF IgG concentration increases either with neurological diseases that alter the blood-brain barrier (BBB), increasing IgG access from the blood, or with neurological diseases that determine local activation of the immune system with intrathecal synthesis (IS) of IgG. To date, no studies on IEF of canine CSF have been performed. The purpose of this study was to investigate IgG patterns of distribution in CSF of dogs with several neurological diseases and to compare these data to CSF total protein concentrations for each dog.. MATERIALS. Twenty-four dogs have been included in the study. Sixteen dogs had spinal cord compression (SCC), 3 dogs had brain neoplasia (BN), 2 dogs had cerebellar abiotrophy (CA), 1 dog had steroid-responsive meningitis ...
http://www.vin.com/apputil/content/defaultadv1.aspx?pId=11147&id=3846453
Turner, US - Patent applicationsTurner, US - Patent applications
Suspended basket system for freezers and portable coolers - The present invention suspended basket system for freezers and portable coolers, includes: (a) an insulating member selected from the group consisting of freezers and portable coolers, the insulating member including a bottom, a back, an open top, and a cover connectable to an outer perimeter of the open top through cover connectable means; (b) a basket hanging member including an open frame nested to the insulating member through open frame nesting means, at least one hinge point rod connected to the open frame, and a suspension mechanism connected to the at least one hinge point rod through suspension mechanism connecting means, the suspension mechanism further connected to at least one basket through at least one basket connecting means wherein the at least one basket is adapted for holding food and beverages normally stored in the insulating member; such that as the open frame is increasingly opened by lifting the open frame ...
http://www.patentsencyclopedia.com/inventor/turner-us-5/
Protein/peptide purification by three-well OFFGEL electrophoresis with immobilized ultra narrow pH gradient gels - Analytical...Protein/peptide purification by three-well OFFGEL electrophoresis with immobilized ultra narrow pH gradient gels - Analytical...
Purification and desalting of protein and peptide samples by three-well OFFGEL electrophoresis with immobilized ultra narrow pH gradient gels is proposed as a fast preparative strategy for proteomics. The gist of this strategy is to separate proteins and peptides according to their isoelectric points and to
https://pubs.rsc.org/en/content/articlelanding/2014/AY/C4AY00088A
ampholyte | Tabers Medical Dictionaryampholyte | Tabers Medical Dictionary
ampholyte answers are found in the Tabers Medical Dictionary powered by Unbound Medicine. Available for iPhone, iPad, Android, and Web.
https://www.tabers.com/tabersonline/view/Tabers-Dictionary/769465/all/ampholytic
IEF Sample Buffer (2x) › Buffer Solutions and Reagents›Electrophoresis › SERVA Electrophoresis GmbHIEF Sample Buffer (2x) › Buffer Solutions and Reagents›Electrophoresis › SERVA Electrophoresis GmbH
SERVA IEF sample buffer is suited to all vertical and horizontal IEF applications and systems. The sample buffer is supplied as 2x concentrate. It is sterile filtered, beneficial to long shelf life and absence of contaminants. Simply mix the liquid sample 1:1 with the buffer or dissolve a solid sample in the buffer first and dilute with water 1:1. When performing IEF in the presence of urea mix the sample with the buffer and add solid urea or use concentrated urea solution.The buffer contains 4 % SERVALYT™ 4 - 9 T, 30 % glycerol and 0.005 % phenol red.
https://serva.de/enDE/ProductDetails/510_42537_IEF_Sample_Buffer_2x_sterile_filtered_0_214.html
Electrolyte retention of supported bi-layered nanofiltration membranes<...
TY - JOUR. T1 - Electrolyte retention of supported bi-layered nanofiltration membranes. AU - de Lint, W.B.S.. AU - Radosevic-Zivkovic, T.. AU - Benes, Nieck Edwin. AU - Bouwmeester, Henricus J.M.. AU - Blank, David H.A.. PY - 2006. Y1 - 2006. N2 - The electrolyte separation behaviour of a supported bi-layered ceramic membrane is investigated experimentally and the measured ion retentions are compared with the predictions of a site-binding transport model with no adjustable parameters. Due to the difference in iso-electric point between its two separating layers, the bi-layered system is expected to perform better over a large pH range compared with a membrane with only one type of selective layer. The separating layers in the membrane are a microporous silica and a mesoporous -alumina (pore sizes of 0.8 and 2 nm, respectively) and their retention is studied for a binary electrolyte solution of NaCl at 1 mol/m3 for pH values between 4 and 10. Because of its smaller pores and high charge, the ...
https://research.utwente.nl/en/publications/electrolyte-retention-of-supported-bi-layered-nanofiltration-memb
Ampholyte Mixtures - Medical Dictionary online-medical-dictionary.orgAmpholyte Mixtures - Medical Dictionary online-medical-dictionary.org
Such mixtures of amphoteric Electrolytes or Buffers that provide a continuous range of pH in an electric field; used for separating Proteins by their Isoelectric Points, i.e., by Isoelectric Focusing ...
http://www.online-medical-dictionary.org/definitions-a/ampholyte-mixtures.html
Phosphoinositide 3 Kinase, p85 alpha (PI3K p85a) ELISA KitsPhosphoinositide 3 Kinase, p85 alpha (PI3K p85a) ELISA Kits
Reactivity: Chicken, Cow, Dog and more. Compare different PI3K p85a ELISA Kits & buy the right one directly at antibodies-online.com!
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Ultrasound QuarterlyUltrasound Quarterly
Ultrasound Quarterly, the official journal of the Society of Radiologists in Ultrasound, provides coverage of the newest, most sophisticated ultrasound techniques as well as in-depth analysis of important developments in this dynamic field.
http://www.ovid.com/product-details.1136.html
pIPredict: A computer tool for prediction of isoelectric points of peptides and proteins | SpringerLinkpIPredict: A computer tool for prediction of isoelectric points of peptides and proteins | SpringerLink
The data on approximate values of isoelectric point (pI) of peptides obtained during their fractionation by isoelectric focusing can be successfully used for the creation of the pKa scale for amino ac
https://link.springer.com/article/10.1134%2FS1990750815030099
MathGroup Archive: January 2001 [00313]MathGroup Archive: January 2001 [00313]
bes ,bes at pi5246.physik.uni-erlangen.de, wrote in message news:94oi30$eg5 at smc.vnet.net... , Hallo, , is it possible to use fractions for a label? , Show[Plot[x,{x,-1,1},DisplayFunction-,Identity], Ticks-,{ {-1,-3/4,-1/2,-1/4,1/4,1/2,3/4,1}, {-1,-3/4,-1/2,-1/4,1/4,1/2,3/4,1} } ,DisplayFunction-,$DisplayFunction] -- Paul Lutus www.arachnoid.com ...
http://forums.wolfram.com/mathgroup/archive/2001/Jan/msg00313.html
Online Business Operator: September 2015Online Business Operator: September 2015
You have been walking for years, but chances are, you havent been utilising all of your muscles. Power walking, also known as speed walking, is a cross between regular walking and jogging. An extreme form of power walking-also called race walking-involves rigorous rules and requirements during timed events. Research suggests that power walking or brisk walking can help reduce the risk of heart disease, stroke, type 2 diabetes, cancer and depression. Although it is a very basic form of exercise, it does require some amount of preparation. Here are a few effective ways to maximise the potential of your power walk. ...
https://online-business-operator.blogspot.com/2015/09/
Core77.com • View topic - Sickening...Core77.com • View topic - Sickening...
BTW, on a side note. I visited a couple of faucet factories in GD recently. Basically the same process. Brass casting the basic form, machining to form flat surfaces for seals, tapping the threads for assembly, polishing the exterior for a smooth finish. Its not that complicated (at least when you are chroming brass ...
http://boards.core77.com/viewtopic.php?f=5&p=197255
SOM-4455 - ECA ServicesSOM-4455 - ECA Services
114 x 95 mm basic form factor. Supplier: Advantech. Part Numbers: SOM-4455R-L0A2E, SOM-4455RL-L0A2E, SOM-4455RL-LSA2E, SOM-4455R-LSA2E,. Category: Single Board Computers. AMD Geode™ LX800 Process with CS5536, ETX CPU Module, 4 PCI Master, ISA, 2 IDE, 2 SATA, 4 USB 2.0, 1 10/100 Ethernet, 2 COM, 1 LPT/FDD. ...
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CryoBioPhysica.com, pISep Advanced Protein Purification Technology
CryoBioPhysica, creators of pISep buffers & pISep Software for separation of proteins by chromatofocusing with controllable external pH gradients.
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pI profiles across HC CDR3 regions of NZM2410-derived A | Open-ipI profiles across HC CDR3 regions of NZM2410-derived A | Open-i
pI profiles across HC CDR3 regions of NZM2410-derived ANAs and non-ANAs. The mean pI value (isoelectric point) at each HC CDR3 position (from H95 to H100a) was
https://openi.nlm.nih.gov/detailedresult.php?img=PMC2211797_20030132f6&req=4
Pier Giorgio RighettiPier Giorgio Righetti
"Chapter 2 Evolution and development of isoelectric focusing". In Garfin, David; Ahuja, Satinder (eds.). Handbook of isoelectric ... Righetti, P. G.; Drysdale, J. W. (1976). Isoelectric focusing. New York: American Elsevier Pub. Co. 2014, AES Award, American ... They developed techniques for isoelectric focusing (IEF) using soluble carrier ampholytes and polyacrylamide gels. In 1971, ... Righetti, P. G. (1983). Isoelectric focusing : theory, methodology, and applications. 1st ed. Laboratory techniques in ...
https://en.wikipedia.org/wiki/Pier_Giorgio_Righetti
QPNC-PAGEQPNC-PAGE
The aim of isoelectric focusing (IEF), for example, is to separate proteins according to their isoelectric point (pI), thus, ... Garfin DE (1990). Isoelectric focusing. Methods in Enzymology. Vol. 182. pp. 459-77. doi:10.1016/0076-6879(90)82037-3. ISBN ... Under standard conditions (69 hr), metalloproteins with different molecular mass ranges and isoelectric points have been ... at continuous pH conditions and different velocities of migration depending on different isoelectric points. The separated ( ...
https://en.wikipedia.org/wiki/QPNC-PAGE
Acid dissociation constantAcid dissociation constant
... so different molecules have different isoelectric points. This permits a technique called isoelectric focusing, which is used ... 2005). Handbook of Isoelectric Focusing and Proteomics. Vol. 7. Elsevier. ISBN 0-12-088752-5. Hulanicki, A. (1987). Reactions ... In the case that there is one species of each type, the isoelectric point can be obtained directly from the pK values. Take the ... For substances in solution, the isoelectric point (pI) is defined as the pH at which the sum, weighted by charge value, of ...
https://en.wikipedia.org/wiki/Acid_dissociation_constant
Eastern blotEastern blot
Reinhart and Malamud; Malamud, D (1982). "Protein transfer from isoelectric focusing gels: the native blot". Analytical ...
https://en.wikipedia.org/wiki/Eastern_blot
Immobilized pH gradientImmobilized pH gradient
IPG increased reproducibility of Isoelectric focusing and 2D-Gel Electrophoresis. Other advantages are increased resolution, ... of protein spots in two-dimensional polyacrylamide gel electrophoresis using immobilised pH gradient isoelectric focusing in ... The gel is then washed to remove catalysts and unpolymerized monomers, which interfere with isoelectric separation. ...
https://en.wikipedia.org/wiki/Immobilized_pH_gradient
BBE-like enzymesBBE-like enzymes
After that, the resulting solution was fractionated using standard procedures and generated, after isoelectric focusing, a ... This value was obtained with isoelectric focusing and chromatofocusing techniques. The enzyme broad temperature range is ... The BBE-like enzyme's optimum pH is 8.9, this means it works in an alkaline medium, but the isoelectric point of the ...
https://en.wikipedia.org/wiki/BBE-like_enzymes
Protein mass spectrometryProtein mass spectrometry
The first-dimension of 2D gel is isoelectric focusing (IEF). In this dimension, the protein is separated by its isoelectric ...
https://en.wikipedia.org/wiki/Protein_mass_spectrometry
ProteinProtein
"Fractionation of complex protein mixtures by liquid-phase isoelectric focusing". 2D PAGE: Sample Preparation and Fractionation ... ISBN 978-0-19-963789-8. Kozlowski LP (January 2017). "Proteome-pI: proteome isoelectric point database". Nucleic Acids Research ... can be monitored using various types of gel electrophoresis if the desired protein's molecular weight and isoelectric point are ...
https://en.wikipedia.org/wiki/Protein
Shotgun proteomicsShotgun proteomics
Klose J (1975). "Protein mapping by combined isoelectric focusing and electrophoresis of mouse tissues. A novel approach to ... It can be used in projects ranging from large-scale whole proteome to focusing on a single protein family. It can be done in ...
https://en.wikipedia.org/wiki/Shotgun_proteomics
Core-shell semiconductor nanocrystalCore-shell semiconductor nanocrystal
"Size-selective separation of gold nanoparticles using isoelectric focusing electrophoresis (IEF)". Chemical Communications (6 ... Clark, Michael D.; Kumar, Sanat K.; Owen, Jonathan S.; Chan, Emory M. (2011-05-11). "Focusing Nanocrystal Size Distributions ...
https://en.wikipedia.org/wiki/Core–shell_semiconductor_nanocrystal
ZingibainZingibain
1995). "Purification of ginger proteases by DEAE-Sepharose and isoelectric focusing". Biochim. Biophys. Acta. 1243 (2): 181-4. ...
https://en.wikipedia.org/wiki/Zingibain
Droplet-based microfluidicsDroplet-based microfluidics
Wang S, Chen S, Wang J, Xu P, Luo Y, Nie Z, Du W (September 2014). "Interface solution isoelectric focusing with in situ MALDI- ... Flow focusing can also be an active method with the constraint point being adjustable using pneumatic side chambers controlled ... Using specific flow-focusing multiphase designs that control flow rate and temperature, the size of nanoparticle formation can ... Hatch AC, Patel A, Beer NR, Lee AP (April 2013). "Passive droplet sorting using viscoelastic flow focusing". Lab on a Chip. 13 ...
https://en.wikipedia.org/wiki/Droplet-based_microfluidics
ACES (buffer)ACES (buffer)
ACES use in isoelectric focusing of proteins has also been documented. Use of ACES has been published in a protocol for the ... Alonso, A., "Human α-1-antitrypsin subtyping by hybrid isoelectric focusing in miniaturized polyacrylamide gel." " ...
https://en.wikipedia.org/wiki/ACES_(buffer)
Two-dimensional gel electrophoresisTwo-dimensional gel electrophoresis
Separation of the proteins by isoelectric point is called isoelectric focusing (IEF). Thereby, a pH gradient is applied to a ... In addition, proteins will not migrate when they have no charge (a result of the isoelectric focusing step) therefore the ... See Isoelectric focusing In quantitative proteomics, these tools primarily analyze bio-markers by quantifying individual ... Klose, J (1975). "Protein mapping by combined isoelectric focusing and electrophoresis of mouse tissues. A novel approach to ...
https://en.wikipedia.org/wiki/Two-dimensional_gel_electrophoresis
TubulinTubulin
Williams RC, Shah C, Sackett D (November 1999). "Separation of tubulin isoforms by isoelectric focusing in immobilized pH ... These subunits are slightly acidic, with an isoelectric point between 5.2 and 5.8. Each has a molecular weight of approximately ...
https://en.wikipedia.org/wiki/Tubulin
Staphylococcus aureus beta toxinStaphylococcus aureus beta toxin
... comparison of three isoelectric focusing methods". Protein Expression and Purification. 9 (1): 76-82. doi:10.1006/prep. ...
https://en.wikipedia.org/wiki/Staphylococcus_aureus_beta_toxin
Alpha-1 antitrypsinAlpha-1 antitrypsin
As protein electrophoresis is imprecise, the A1AT phenotype is analysed by isoelectric focusing (IEF) in the pH range 4.5-5.5, ... this causes the heterogeneity observed on normal A1AT when analysed by isoelectric focusing. Also, the fucosylated triantennary ... where the protein migrates in a gel according to its isoelectric point or charge in a pH gradient. Normal A1AT is termed M, as ...
https://en.wikipedia.org/wiki/Alpha-1_antitrypsin
Amy HerrAmy Herr
Her thesis is titled "Isoelectric Focusing for Multi-Dimensional Separations in Microfluidic Devices."[citation needed] After ...
https://en.wikipedia.org/wiki/Amy_Herr
MetaproteomicsMetaproteomics
250, 4007-4021 (1974). Klose, J. Protein mapping by combined isoelectric focusing and electrophoresis of mouse tissues. A novel ...
https://en.wikipedia.org/wiki/Metaproteomics
AmyloidosisAmyloidosis
ATTR can be identified using isoelectric focusing which separates mutated forms of transthyretin. Findings can be corroborated ...
https://en.wikipedia.org/wiki/Amyloidosis
AmphoterismAmphoterism
Ampholytes are used to establish a stable pH gradient for use in isoelectric focusing. Metal oxides which react with both acids ... Ate complex Isoelectric point Zwitterion IUPAC, Compendium of Chemical Terminology, 2nd ed. (the "Gold Book") (1997). Online ... The pH at which the average charge is zero is known as the molecule's isoelectric point. ...
https://en.wikipedia.org/wiki/Amphoterism
Bio-MEMSBio-MEMS
Isoelectric focusing is the separation of proteins, organelles, and cells with different isoelectric points. Isoelectric ... Examples of continuous-flow separation techniques include continuous-flow electrophoresis, isoelectric focusing, continuous- ... Sorting and focusing of the species of interest is achieved because an electrophoretic force causes perpendicular migration ... Thus, companies focusing on tropical diseases must often combine their research objectives for tropical disease with research ...
https://en.wikipedia.org/wiki/Bio-MEMS
ESD (gene)ESD (gene)
1987). "Genetic study of red cell esterase D polymorphism by ultrathin layer isoelectric focusing. Distribution in the Veneto ...
https://en.wikipedia.org/wiki/ESD_(gene)
SDS-PAGESDS-PAGE
Two-dimensional gel electrophoresis sequentially combines isoelectric focusing or BAC-PAGE with a SDS-PAGE. Native PAGE is used ...
https://en.wikipedia.org/wiki/SDS-PAGE
Siamese fighting fishSiamese fighting fish
"Genetic variation in different varieties of Siamese fighting fish using isoelectric focusing of sarcoplasmic proteins". ...
https://en.wikipedia.org/wiki/Siamese_fighting_fish
GliadinGliadin
... s are prolamins and are separated on the basis of electrophoretic mobility and isoelectric focusing. Gliadin peptides ...
https://en.wikipedia.org/wiki/Gliadin
Luschans salamanderLuschan's salamander
There were around ten molecular forms of esterases present based on isoelectric focusing and gel electrophoresis. The main two ...
https://en.wikipedia.org/wiki/Luschan's_salamander
TrypsinogenTrypsinogen
"Characterization of human exocrine pancreatic proteins by two-dimensional isoelectric focusing/sodium dodecyl sulfate gel ...
https://en.wikipedia.org/wiki/Trypsinogen
Oligoclonal bandOligoclonal band
New techniques like "capillary isoelectric focusing immunoassay" are able to detect IgG OCBs in more than 95% of multiple ... the combination of isoelectric focusing/silver staining. The latter is more sensitive. For the analysis of cerebrospinal fluid ... "Detection of intrathecal immunoglobulin G synthesis by capillary isoelectric focusing immunoassay in oligoclonal band negative ... of virus-specific oligoclonal bands in subacute sclerosing panencephalitis by immunofixation after isoelectric focusing and ...
https://en.wikipedia.org/wiki/Oligoclonal_band
Hemoglobin electrophoresisHemoglobin electrophoresis
Newer alternatives to conventional hemoglobin electrophoresis include isoelectric focusing, capillary zone electrophoresis, and ...
https://en.wikipedia.org/wiki/Hemoglobin_electrophoresis
ADA (buffer)ADA (buffer)
... and for isoelectric focusing in immobilized pH gradients. Its effects on dog kidney Na+/K+-ATPase and rat brain GABA receptors ...
https://en.wikipedia.org/wiki/ADA_(buffer)
C12orf50C12orf50
This page is focusing on isoform X1. C12orf50 isoform X1 is 1711 nucleotides long and has a protein with a length of 414 aa. ... The predicted molecular weight of 47.3 kdal and an isoelectric point of 8.79. The predicted tertiary structure for C12orf50 has ...
https://en.wikipedia.org/wiki/C12orf50
Congenital disorder of glycosylationCongenital disorder of glycosylation
The most commonly used screening method for CDG, analysis of transferrin glycosylation status by isoelectric focusing, ESI-MS, ... isoelectric focusing), the new syndrome was named carbohydrate-deficient glycoprotein syndrome (CDGS) Its "classical" phenotype ...
https://en.wikipedia.org/wiki/Congenital_disorder_of_glycosylation
C1orf112C1orf112
... has a determined precursor molecular weight of 96.6 kDa and an isoelectric point of 5.62. C1orf112 has been ... "The focusing positions of polypeptides in immobilized pH gradients can be predicted from their amino acid sequences". ...
https://en.wikipedia.org/wiki/C1orf112
Difference gel electrophoresisDifference gel electrophoresis
The binary shifts might be left to right (change in isoelectric point), vertical (change in size) or diagonal (change in both ... The three samples are mixed and loaded onto IEF (isolectric focusing chromatography) for first dimension and the strip is ... truncations and any modification that might change the size or isoelectric point of proteins. ... size and isoelectric point). Reciprocal Labeling is done to make sure the changes seen are not due to dye-dependent ...
https://en.wikipedia.org/wiki/Difference_gel_electrophoresis
Capillary electrophoresis-mass spectrometryCapillary electrophoresis-mass spectrometry
... including capillary isotachophoresis and capillary isoelectric focusing. There are two common techniques to load the sample ...
https://en.wikipedia.org/wiki/Capillary_electrophoresis–mass_spectrometry
Troponin C type 1Troponin C type 1
Kobayashi T, Yang X, Walker LA, Van Breemen RB, Solaro RJ (Jan 2005). "A non-equilibrium isoelectric focusing method to ...
https://en.wikipedia.org/wiki/Troponin_C_type_1
Liguri MosulishviliLiguri Mosulishvili
MATRICE OF Spirulina platensis Determination of the pI of Human Rhinovirus Serotype 2 by Capillary Isoelectric Focusing The ...
https://en.wikipedia.org/wiki/Liguri_Mosulishvili
John Arbuthnott (microbiologist)John Arbuthnott (microbiologist)
July 2000 Isoelectric Focusing. 1975. The Determinants of Bacterial and Viral Pathogenicity. 1983. Foodborne Illness: a Lancet ...
https://en.wikipedia.org/wiki/John_Arbuthnott_(microbiologist)
List of MeSH codes (H01)List of MeSH codes (H01)
... isoelectric focusing MeSH H01.181.529.307.719 - polarography MeSH H01.181.529.307.794 - potentiometry MeSH H01.181.529.328 - ... isoelectric point MeSH H01.181.529.444 - hydrolysis MeSH H01.181.529.450 - hydrophobicity MeSH H01.181.529.479 - ion exchange ...
https://en.wikipedia.org/wiki/List_of_MeSH_codes_(H01)
Newborn screeningNewborn screening
... for a large number of hemoglobinopathies is done by detecting abnormal patterns using isoelectric focusing, ...
https://en.wikipedia.org/wiki/Newborn_screening
ProteomeProteome
In the first dimension, the proteins are separated by isoelectric focusing, which resolves proteins on the basis of charge. In ... Kozlowski, LP (26 October 2016). "Proteome-pI: proteome isoelectric point database". Nucleic Acids Research. 45 (D1): D1112- ...
https://en.wikipedia.org/wiki/Proteome
IEFIEF
International Energy Forum Isoelectric focusing Islamic Enlightenment Foundation International e-Sports Festival This ...
https://en.wikipedia.org/wiki/IEF
Arcopilus aureusArcopilus aureus
However, using isoelectric focusing in polyacrylamide gels to compare esterase enzymes in isolates of the Chaetomium species ...
https://en.wikipedia.org/wiki/Arcopilus_aureus
Ccdc60Ccdc60
The computational isoelectric point of CCDC60 is 9.17 and the computational molecular weight is approximately 63kDa. Western ... Bjellqvist B, Hughes GJ, Pasquali C, Paquet N, Ravier F, Sanchez JC, Frutiger S, Hochstrasser D (October 1993). "The focusing ...
https://en.wikipedia.org/wiki/Ccdc60
ElectrohydrodynamicsElectrohydrodynamics
... field amplified sample stacking and isoelectric focusing), multidimensional assays, and systems with poorly specified sample ...
https://en.wikipedia.org/wiki/Electrohydrodynamics
STS-26STS-26
Isoelectric Focusing Experiment (IFE) Mesoscale Lightning Experiment (MLE) Phase Partitioning Experiment (PPE) Earth-Limb ...
https://en.wikipedia.org/wiki/STS-26
Alpha-1 antitrypsin deficiencyAlpha-1 antitrypsin deficiency
A1AT is further analyzed by isoelectric focusing (IEF) in the pH range 4.5-5.5, where the protein migrates in a gel according ... to its isoelectric point or charge in a pH gradient. Normal A1AT is termed M, as it migrates toward the center of such an IEF ...
https://en.wikipedia.org/wiki/Alpha-1_antitrypsin_deficiency
ACP2ACP2
Radzun HJ, Parwaresch MR (1981). "Isoelectric focusing pattern of acid phosphatase and acid esterase in human blood cells, ...
https://en.wikipedia.org/wiki/ACP2
Isoelectric FocusingIsoelectric Focusing
In order to ensure the high performance of analysis, isoelectric point (pI) standards are needed. ... Information on Isoelectric Focusing including what it is and how it is used. ... What is Isoelectric Focusing?. Isoelectric focusing (IEF) is a powerful analytical tool for the separation of proteins. In ... Horká M, Willimann T, Blum M, Nording P, Friedl Z, ?lais K. 2001. Capillary isoelectric focusing with UV-induced fluorescence ...
https://www.sigmaaldrich.com/MY/en/technical-documents/technical-article/protein-biology/protein-structural-analysis/isoelectric-focusing
Purification of N-Acetylgalactosaminidase by Isoelectric Focusing.
The chief emphasis of the work with Clostridial Azyme was its purification by isoelectric focusing. Any bacterial enzyme ... The chief emphasis of the work with Clostridial Azyme was its purification by isoelectric focusing. Any bacterial enzyme ...
https://apps.dtic.mil/sti/citations/ADA127059
Coupling Capillary Isoelectric Focusing with Mass SpectrometryCoupling Capillary Isoelectric Focusing with Mass Spectrometry
... column discusses current work in the use of capillary isoelectric focusing and mass spectrometry as a multidimensional ... This monthss Directions in Discovery column discusses current work in the use of capillary isoelectric focusing and mass ... Coupling Capillary Isoelectric Focusing with Mass Spectrometry. .social-ris-container { display: flex; justify-content: space- ...
https://www.chromatographyonline.com/view/coupling-capillary-isoelectric-focusing-mass-spectrometry
Instrumental and method development for multidimensional electrophoretic separations - coupling of capillary isoelectric...Instrumental and method development for multidimensional electrophoretic separations - coupling of capillary isoelectric...
The optimized two-dimensional setup was used for the hyphenation of capillary isoelectric focusing to mass spectrometry using a ... coupling of capillary isoelectric focusing to mass spectrometry. DSpace Repository. Login ... coupling of capillary isoelectric focusing to mass spectrometry. Sydes, Daniel ... was achieved for fluorescein isothiocyanate and labeled model proteins using flushing and capillary isoelectric focusing (CIEF ...
https://publikationen.uni-tuebingen.de/xmlui/handle/10900/75703
isoelectric focusing Tenders in Biharisoelectric focusing Tenders in Bihar
Search isoelectric focusing Tenders from Bihar State. View Tenders published by Government of Bihar for isoelectric focusing. ... Find latest isoelectric focusing Tenders in Bihar. ...
http://www.punjabtenders.net/SearchTender?Keyword=isoelectric+focusing
Emergence of Ceftriaxone-Resistant Salmonella Isolates and Rapid Spread of Plasmid-Encoded CMY-2-Like Cephalosporinase, Taiwan ...
Isoelectric focusing showed that both isolates expressed two β-lactamases focusing at pIs 5.4 and 9.0, suggesting that they ... Isoelectric Focusing. Crude β-lactamase extracts were prepared by using sonication (27) as described previously (23). We ... The use of analytical isoelectric focusing for detection and identification of β-lactamases. J Gen Microbiol. 1975;88:169-78. ... performed isoelectric focusing by the method of Matthew et al. (28) with an LKB Multiphor apparatus on prepared PAGplate gels ( ...
https://wwwnc.cdc.gov/eid/article/9/3/01-0410
Transferrin subtypes determined by ultrathin‐layer polyacrylamide gel isoelectric focusing<...
... layer polyacrylamide gel isoelectric focusing",. abstract = "An ultrathin‐layer polyacrylamide gel isoelectric focusing method ... Budowle B. Transferrin subtypes determined by ultrathin‐layer polyacrylamide gel isoelectric focusing. Electrophoresis. 1985 ... Budowle, B. (1985). Transferrin subtypes determined by ultrathin‐layer polyacrylamide gel isoelectric focusing. Electrophoresis ... Budowle, B 1985, Transferrin subtypes determined by ultrathin‐layer polyacrylamide gel isoelectric focusing, Electrophoresis ...
https://experts.unthsc.edu/en/publications/transferrin-subtypes-determined-by-ultrathinlayer-polyacrylamide-
kappa Free Light Chains Index as a Screening Test for MSkappa Free Light Chains Index as a Screening Test for MS
... using isoelectric focusing (IEF) followed by immunofixation is considered a hallmark of MS testing. This modality is helpful ... OCBs were detected by isoelectric focusing, followed by immunofixation. Cerebrospinal fluid (CSF) and serum κFLC were measured ...
http://www.medscape.com/viewarticle/934024
Cephalosporin-resistant Escherichia coli among Summer Camp Attendees with Salmonellosis - Volume 9, Number 10-October 2003 -...
Extraction of β-Lactamases and Isoelectric Focusing (IEF). Crude extracts of β-lactamases were obtained by ultrasonication. ... Beta-lactamases: determination of their isoelectric points. Antimicrob Agents Chemother. 1978;13:695-8.PubMedGoogle Scholar ... In the 11 E. coli isolates phenotypically suspected of ESBL production, a β-lactamase with an isoelectric point of 8.0 was ...
https://wwwnc.cdc.gov/eid/article/9/10/03-0179
Comparison of electrophoresis, isoelectric focusing, and immunodiffusion in determinations of cows and goats milk in Serra da...Comparison of electrophoresis, isoelectric focusing, and immunodiffusion in determinations of cow's and goat's milk in Serra da...
Comparison of electrophoresis, isoelectric focusing, and immunodiffusion in determinations of cows and goats milk in Serra da ... Identification and quantitative determination of cows milk in ewes cheeses using isoelectric focusing of wheys Lait 71, 501- ... The quantitative results obtained by electrophoresis and isoelectric focusing did not show any significant differences (P , ... Abstract - A comparative study of electrophoresis (PAGE), isoelectric focusing (IEF), and radial immunodiffusion (RID) in ...
https://lait.dairy-journal.org/articles/lait/abs/1992/01/lait_72_1992_1_7/lait_72_1992_1_7.html
Different distribution of thiaminpyrophosphatase activity in neuronal and glial cell enriched fractions from human and rat...
... an isoelectric focusing investigation.. LAFORENZA, UMBERTO;MAZZARELLO, PAOLO ANGELO;PATRINI C;POLONI M;CASADEI GP;RINDI G. 1990 ... activity by isoelectric focusing (IEF). Glial and neuronal fractions yielded about 0.16 and 0.03 g/g of wet tissue respectively ... activity by isoelectric focusing (IEF). Glial and neuronal fractions yielded about 0.16 and 0.03 g/g of wet tissue respectively ... TPPase activity was present in 10 distinct protein bands with different isoelectric points. These preliminary data suggest that ...
https://iris.unipv.it/handle/11571/108729
Analysis of polypeptides of the tree shrew (Tupaia) herpesvirus by gel electrophoresisAnalysis of polypeptides of the tree shrew (Tupaia) herpesvirus by gel electrophoresis
... was analysed by SDS-polyacrylamide slab gel electrophoresis and by a two-dimensional technique using isoelectric focusing. Two ... was analysed by SDS-polyacrylamide slab gel electrophoresis and by a two-dimensional technique using isoelectric focusing. Two ...
https://pubmed.ncbi.nlm.nih.gov/7142964/
Differential Protein Expression between Chondrogenic Differentiated MSCs, Undifferentiated MSCs and Adult Chondroctyes Derived...Differential Protein Expression between Chondrogenic Differentiated MSCs, Undifferentiated MSCs and Adult Chondroctyes Derived...
Isoelectric focusing (IEF) and Two-dimensional Gel Electrophoresis (2-DE). Prior to IEF, cell lysate was precipitated in ... Isoelectric focusing; LC-MS: Liquid chromatography-mass spectrometry; MSC: Multipotent stromal cells; MYL6B: Myosin light chain ... pI referred to theoretical Isoelectric point of the protein; (f) sequence coverage (%) meant sequence coverage in percentage ...
https://medsci.org/v11p0024.htm
Accumulation of prohibitin is a common cellular response to different stressing stimuli and protects melanoma cells from ER...Accumulation of prohibitin is a common cellular response to different stressing stimuli and protects melanoma cells from ER...
Isoelectric focusing (IEF) and SDS-PAGE. An IPG-Phor electrophoretic system was used with 18 cm pH 4-7 gradient strips (GE ... The Table lists 34 regulated proteins identified by MALDI-TOF which were detected as 62 isoelectric forms. n.d (not detected) ... The 34 regulated proteins identified by mass spectrometry, which comprise 62 isoelectric forms, are listed in Table 1. ...
https://www.oncotarget.com/article/17810/text/
Science Clips - Volume 10, Issue 12, April 3, 2018Science Clips - Volume 10, Issue 12, April 3, 2018
Isoelectric focusing studies demonstrated three beta-lactamases, with pIs of 7.2 (SHV-29), 6.7 (KPC-1), and 5.4 (TEM-1). The ...
https://www.cdc.gov/library/sciclips/issues/v10issue12.html
Volume 106, Issue 2 | Microbiology SocietyVolume 106, Issue 2 | Microbiology Society
Isoelectric Focusing Studies on the ?-Fructofuranosidases and ?-Glucosidases of Streptococcus mitis Marie-Louise Sund, Lars ... glucosidase activities by isoelectric focusing in thin-layer polyacrylamide gels combined with zymogram procedures. Three bands ...
https://www.microbiologyresearch.org/content/journal/micro/106/2
MeSH BrowserMeSH Browser
Isoelectric Focusing [E05.301.300.663] * Capillary Isoelectric Focusing [E05.301.300.663.250] * Isoelectric Point [E05.301. ... at which the pH is equal to their isoelectric point.. Terms. Isoelectric Focusing Preferred Term Term UI T022621. Date01/01/ ... Isoelectric Focusing Preferred Concept UI. M0011756. Scope Note. Electrophoresis in which a pH gradient is established in a gel ... Isoelectric Focusing. Tree Number(s). E05.196.401.663. E05.301.300.663. Unique ID. D007525. RDF Unique Identifier. http://id. ...
https://meshb.nlm.nih.gov/record/ui?ui=D007525
SearchSearch
Thomas W. Kenny - PublicationsThomas W. Kenny - Publications
On-chip coupling of isoelectric focusing and free solution electrophoresis for multidimensional separations. Analytical ...
https://academictree.org/bme/publications.php?pid=165698
Výpis publikacíVýpis publikací
The capillary isoelectric focusing and direct microscopy. In Proceedings of the World Conference of Dosage Antiinfectives. ... The capillary isoelectric focusing and direct microscopy. HOLÁ, Veronika, Filip RŮŽIČKA, Marie HORKÁ a Karel ŠLAIS. Methods of ... Capillary Isoelectric Focusing-Useful Tool for Detection and Quantification of Lactic Acid Bacteria in Milk. Food Analytical ... Capillary isoelectric focusing of native and inactivated microorganisms. HORKA, Marie, O. KUBICEK, Filip RŮŽIČKA, Veronika HOLÁ ...
https://is.muni.cz/publications/77354
Biopharmaceutical Analytical Testing - A Critical Step in Producing Cutting-Edge TherapiesBiopharmaceutical Analytical Testing - A Critical Step in Producing Cutting-Edge Therapies
Polyacrylamide gel electrophoresis, isoelectric focusing, SDS-polyacrylamide gel electrophoresis, western-blot, capillary ...
https://www.technologynetworks.com/cell-science/articles/biopharmaceutical-analytical-testing-a-critical-step-in-producing-cutting-edge-therapies-365496
Rapid Development and Scale-Up of Biosimilar TrastuzumabBioProcess InternationalRapid Development and Scale-Up of Biosimilar TrastuzumabBioProcess International
cIEF: Capillary isoelectric focusing. DSC: Differential scanning calorimetry. ELISA: Enzyme-linked immunosorbent assay. FL-HPLC ... and capillary isoelectric focusing (cIEF) to confirm quality based on size variants and charge variants. Finally, we have ... capillary isoelectric focusing (cIEF) for charge variants, and N-glycan analysis for glycosylation profiles. Although purity ... and charge variants measured by capillary isoelectric focusing (cIEF) from Cook Pharmicas 20-L run compared with the innovator ...
https://bioprocessintl.com/manufacturing/biosimilars/rapid-development-and-scale-up-of-biosimilar-trastuzumab-a-case-study-of-integrated-cell-line-and-process-development/
Yanhong Liu : USDA ARSYanhong Liu : USDA ARS
pH fractionation and identification of proteins: comparing column isoelectric focusing vs liquid based focusing techniques - ( ... pH fractionation and identification of proteins: comparing column isoelectric focusing vs liquid based focusing techniques. ...
https://www.ars.usda.gov/people-locations/person?person-id=35470
A Potent Peroxidase from Solid Cell Culture of Ocimum Basilicum with High Sensitivity for Blood Glucose Determination |...A Potent Peroxidase from Solid Cell Culture of Ocimum Basilicum with High Sensitivity for Blood Glucose Determination |...
To run a high-resolution 2D gel electrophoresis, isoelectric focusing (IEF) was first performed as described earlier (Farhadi ...
https://www.researchsquare.com/article/rs-227761/v1
NIOSHTIC-2 Search Results - Full View
https://www.hematology.org/education/trainees/fellows/trainee-news/2021/hemoglobin-electrophoresis-in-sickle-cell-diseasehttps://www.hematology.org/education/trainees/fellows/trainee-news/2021/hemoglobin-electrophoresis-in-sickle-cell-disease
Long-term adaptive evolution of Leuconostoc mesenteroides for enhancement of lactic acid tolerance and production |...Long-term adaptive evolution of Leuconostoc mesenteroides for enhancement of lactic acid tolerance and production |...
... isoelectric focusing (IEF) was carried out on an IPGpore, focused for 55,000 V∙h. After IEF, each strip was incubated for 15 ...
https://biotechnologyforbiofuels.biomedcentral.com/articles/10.1186/s13068-016-0662-3
Bioconjugation ServicesBioconjugation Services
Isoelectric Focusing. Potency Determination by Specific Elisa. Mole ratio determination for total chelate or available chelate ...
https://www.macrocyclics.com/services/bioconjugation-services/
ElectrophoresisHigh-performance liMass spectrometryCerebrospinal fluidPolyacrylamide gelCIEFSeparationProteinsPurificationGelsFractionsEnzymePointQuantitativeMethodAnalysisActivityPointsCellsComplete
Electrophoresis6
  • One of the most critical issues in isoelectric focusing, whether in capillary electrophoresis or slab gel, is standardization of results. (sigmaaldrich.com)
  • Budowle, B 1985, ' Transferrin subtypes determined by ultrathin‐layer polyacrylamide gel isoelectric focusing ', Electrophoresis , vol. 6, no. 2, pp. 97-99. (unthsc.edu)
  • Abstract - A comparative study of electrophoresis (PAGE), isoelectric focusing (IEF), and radial immunodiffusion (RID) in determinations of cow's and goat's milks in Serra da Estrela cheeses was carried out. (dairy-journal.org)
  • The virion polypeptide composition of three independently isolated tree shrew herpesviruses (THV) was analysed by SDS-polyacrylamide slab gel electrophoresis and by a two-dimensional technique using isoelectric focusing. (nih.gov)
  • Electrophoresis in which a pH gradient is established in a gel medium and proteins migrate until they reach the site (or focus) at which the pH is equal to their isoelectric point. (nih.gov)
  • These include gel electrophoresis, high-performance liquid chromatography (HPLC), capillary electrophoresis, and isoelectric focusing. (hematology.org)
High-performance li1
  • Most state-based screening programs in the United States use thin-layer isoelectric focusing or high-performance liquid chromatography techniques for testing. (aafp.org)
Mass spectrometry2
  • This month'ss 'Directions in Discovery' column discusses current work in the use of capillary isoelectric focusing and mass spectrometry as a multidimensional separation technique for proteomic studies. (chromatographyonline.com)
  • The optimized two-dimensional setup was used for the hyphenation of capillary isoelectric focusing to mass spectrometry using a 2D CIEF/CE-MS setup. (uni-tuebingen.de)
Cerebrospinal fluid2
  • Detection of oligoclonal bands (OCBs) in cerebrospinal fluid (CSF) using isoelectric focusing (IEF) followed by immunofixation is considered a hallmark of MS testing. (medscape.com)
  • 3. Positive findings in a cerebrospinal fluid (CSF) specimen (isoelectric focusing evidence of oligoclonal bands and/or elevated immunoglobulin (Ig) G index) [If a recent CSF sample or results from a previous CSF test from each patient is not available, this data will be considered missing information]. (who.int)
Polyacrylamide gel1
  • An ultrathin‐layer polyacrylamide gel isoelectric focusing method is described for subtyping transferrin. (unthsc.edu)
CIEF2
  • In this regard, desired sensitivity as well as spectral and spatial resolution was achieved for fluorescein isothiocyanate and labeled model proteins using flushing and capillary isoelectric focusing (CIEF) experiments. (uni-tuebingen.de)
  • Other techniques that can be performed with CE instruments, such as capillary isoelectric focusing (CIEF), capillary isotachophoresis (CITP), and capillary electrochromatography (CEC), are briefly discussed. (degruyter.com)
Separation4
  • Isoelectric focusing (IEF) is a powerful analytical tool for the separation of proteins. (sigmaaldrich.com)
  • This method produces 10 fractions which can be analyzed on 1-D gels or by LC/MS. In-solution focusing may provide an excellent separation method for proteins, such as membrane proteins or very large proteins that are difficult to handle by other methods. (unr.edu)
  • Evaluation of protein loading techniques and improved separation in OFFGEL isoelectric focusing. (mpg.de)
  • Inactivation of the catalytic activity with organophosphate pesticides did not change the isoelectric point of the isozymes, and therefore separation of native from organophosphate inhibited AChE did not occur. (cdc.gov)
Proteins3
  • We have an Agilent Offgel fractionator capable of separating either intact proteins or protease fragments into as many as 24 fractions based on isoelectric point. (unr.edu)
  • A second method for in-solution isoelectric focusing of proteins is use of our Bio-Rad microRotofor Cell. (unr.edu)
  • The Collaborative Protein Technologies Resource (CPTR) has extensive experience in the characterization of proteins for post-translational modifications and can assist PI's with the Nanopro isoelectric focusing immunoassays which can profile in 6-24 ng of total cell lysate protein the percent phosphorylation status of a given protein using an antibody specific to the unmodified protein. (cancer.gov)
Purification2
  • Purification of N-Acetylgalactosaminidase by Isoelectric Focusing. (dtic.mil)
  • The chief emphasis of the work with Clostridial Azyme was its purification by isoelectric focusing. (dtic.mil)
Gels2
  • glucosidase activities by isoelectric focusing in thin-layer polyacrylamide gels combined with zymogram procedures. (microbiologyresearch.org)
  • Isoelectric focusing was performed on freeze-thaw extracts on polyacrylamide gels as described ( 4 ). (cdc.gov)
Fractions2
  • Different distribution of thiaminpyrophosphatase activity in neuronal and glial cell enriched fractions from human and rat brain: an isoelectric focusing investigation. (unipv.it)
  • Enriched fractions of neuronal and glial cells from rat and human (autoptical specimens of frontal cortex) brains were assayed for thiaminpyrophosphatase (TPPase) activity by isoelectric focusing (IEF). (unipv.it)
Enzyme1
  • In addition, gliadin zymography was conducted to obtain the approximate molecular weights and pH activity profiles of the gliadin-degrading oral enzymes and liquid iso-electric focusing was performed to establish overall enzyme iso-electric points. (greenmedinfo.com)
Point2
  • In order to ensure the high performance of analysis, isoelectric point (pI) standards are needed. (sigmaaldrich.com)
  • Isoelectric point: approx. (biomeda.com)
Quantitative1
  • In 1989, a capillary isoelectric focusing method was developed for quantitative measurement of CDT. (degruyter.com)
Method2
  • An alternative method for separating by charge is in-solution isoelectric focusing. (unr.edu)
  • Alpha1-antitrypsin phenotype determined by isoelectric focusing (IEF) is the most commonly used method to definitively detect the alpha1-antitrypsin phenotype that indicates a risk for AATD. (medscape.com)
Analysis1
  • Proteomic analysis of the lymphocyte plasma membrane using cell surface biotinylation and solution-phase isoelectric focusing. (ox.ac.uk)
Activity1
  • After IEF, TPPase activity was present in 10 distinct protein bands with different isoelectric points. (unipv.it)
Points1
  • Liquid iso-electric focusing indicated that most gliadin-degrading enzymes are acidic in nature with iso-electric points between 2.5 and 4.0. (greenmedinfo.com)
Cells1
  • Isoelectric focusing indicated that cells cultured in the presence of LSD secreted discrete focusing components of pI 4.9 and 5.2. (erowid.org)
Complete1
  • After focusing is complete, the solutes are pumped out of the capillary. (sigmaaldrich.com)
Emergence of Ceftriaxone-Resistant Salmonella Isolates and Rapid Spread of Plasmid-Encoded CMY-2-Like Cephalosporinase, Taiwan ...
Emergence of Ceftriaxone-Resistant Salmonella Isolates and Rapid Spread of Plasmid-Encoded CMY-2-Like Cephalosporinase, Taiwan ... (wwwnc.cdc.gov)
Kappa Free Light Chains Index as a Screening Test for MS
Kappa Free Light Chains Index as a Screening Test for MS (medscape.com)
Cephalosporin-resistant Escherichia coli among Summer Camp Attendees with Salmonellosis - Volume 9, Number 10-October 2003 -...
Cephalosporin-resistant Escherichia coli among Summer Camp Attendees with Salmonellosis - Volume 9, Number 10-October 2003 -... (wwwnc.cdc.gov)
Cephamycin Resistance in Clinical Isolates and Laboratory-derived Strains of Escherichia coli, Nova Scotia, Canada - Volume 9,...
Cephamycin Resistance in Clinical Isolates and Laboratory-derived Strains of Escherichia coli, Nova Scotia, Canada - Volume 9,... (wwwnc.cdc.gov)
NmcA Carbapenem-hydrolyzing Enzyme in Enterobacter cloacae in North America - Volume 9, Number 8-August 2003 - Emerging...
NmcA Carbapenem-hydrolyzing Enzyme in Enterobacter cloacae in North America - Volume 9, Number 8-August 2003 - Emerging... (wwwnc.cdc.gov)
Figure 3 - NmcA Carbapenem-hydrolyzing Enzyme in Enterobacter cloacae in North America - Volume 9, Number 8-August 2003 -...
Figure 3 - NmcA Carbapenem-hydrolyzing Enzyme in Enterobacter cloacae in North America - Volume 9, Number 8-August 2003 -... (wwwnc.cdc.gov)
VEB-1 Extended-Spectrum β-lactamase-producing Acinetobacter baumannii, France - Volume 12, Number 8-August 2006 - Emerging...
VEB-1 Extended-Spectrum β-lactamase-producing Acinetobacter baumannii, France - Volume 12, Number 8-August 2006 - Emerging... (wwwnc.cdc.gov)
Cephalosporin and Ciprofloxacin Resistance in Salmonella, Taiwan - Volume 11, Number 6-June 2005 - Emerging Infectious Diseases...
Cephalosporin and Ciprofloxacin Resistance in Salmonella, Taiwan - Volume 11, Number 6-June 2005 - Emerging Infectious Diseases... (wwwnc.cdc.gov)
Differential Protein Expression between Chondrogenic Differentiated MSCs, Undifferentiated MSCs and Adult Chondroctyes Derived...
Differential Protein Expression between Chondrogenic Differentiated MSCs, Undifferentiated MSCs and Adult Chondroctyes Derived... (medsci.org)
Carbapenem Resistance in Klebsiella pneumoniae Not Detected by Automated Susceptibility Testing - Volume 12, Number 8-August...
Carbapenem Resistance in Klebsiella pneumoniae Not Detected by Automated Susceptibility Testing - Volume 12, Number 8-August... (wwwnc.cdc.gov)
Identification of Residual Blood Proteins in Ticks by Mass Spectrometry Proteomics - Volume 14, Number 8-August 2008 - Emerging...
Identification of Residual Blood Proteins in Ticks by Mass Spectrometry Proteomics - Volume 14, Number 8-August 2008 - Emerging... (wwwnc.cdc.gov)
Coupling Capillary Isoelectric Focusing with Mass Spectrometry
Coupling Capillary Isoelectric Focusing with Mass Spectrometry (chromatographyonline.com)
MeSH Browser
MeSH Browser (meshb.nlm.nih.gov)
Thomas W. Kenny - Publications
Thomas W. Kenny - Publications (academictree.org)
Volume 9, Number 8-August 2003 - Emerging Infectious Diseases journal - CDC
Volume 9, Number 8-August 2003 - Emerging Infectious Diseases journal - CDC (wwwnc.cdc.gov)
Cell Cultivation Process Transfer and Scale-Up - BioProcess InternationalBioProcess International
Cell Cultivation Process Transfer and Scale-Up - BioProcess InternationalBioProcess International (bioprocessintl.com)
A Potent Peroxidase from Solid Cell Culture of Ocimum Basilicum with High Sensitivity for Blood Glucose Determination |...
A Potent Peroxidase from Solid Cell Culture of Ocimum Basilicum with High Sensitivity for Blood Glucose Determination |... (researchsquare.com)
WHO EMRO | Identification of immunoreactive proteins in secretions of Leishmania infantum promastigotes: an immunoproteomic...
WHO EMRO | Identification of immunoreactive proteins in secretions of Leishmania infantum promastigotes: an immunoproteomic... (emro.who.int)
Long-term adaptive evolution of Leuconostoc mesenteroides for enhancement of lactic acid tolerance and production |...
Long-term adaptive evolution of Leuconostoc mesenteroides for enhancement of lactic acid tolerance and production |... (biotechnologyforbiofuels.biomedcentral.com)
Bioconjugation Services
Bioconjugation Services (macrocyclics.com)
Alpha1-Antitrypsin (AAT) Deficiency Workup: Approach Considerations, Laboratory Studies, Imaging Studies
Alpha1-Antitrypsin (AAT) Deficiency Workup: Approach Considerations, Laboratory Studies, Imaging Studies (medscape.com)
Genetic Background of Escherichia coli and Extended-spectrum β-Lactamase Type - Volume 11, Number 1-January 2005 - Emerging...
Genetic Background of Escherichia coli and Extended-spectrum β-Lactamase Type - Volume 11, Number 1-January 2005 - Emerging... (wwwnc.cdc.gov)