Antibodies from an individual that react with ISOANTIGENS of another individual of the same species.
Works containing information articles on subjects in every field of knowledge, usually arranged in alphabetical order, or a similar work limited to a special field or subject. (From The ALA Glossary of Library and Information Science, 1983)
Lists of words, usually in alphabetical order, giving information about form, pronunciation, etymology, grammar, and meaning.
The addition of descriptive information about the function or structure of a molecular sequence to its MOLECULAR SEQUENCE DATA record.
Databases devoted to knowledge about specific genes and gene products.
The flexible rope-like structure that connects a developing FETUS to the PLACENTA in mammals. The cord contains blood vessels which carry oxygen and nutrients from the mother to the fetus and waste products away from the fetus.
Union of the fragments of a fractured bone in a faulty or abnormal position. If two bones parallel to one another unite by osseous tissue, the result is a crossunion. (From Manual of Orthopaedic Terminology, 4th ed)
High-amplitude compression waves, across which density, pressure, and particle velocity change drastically. The mechanical force from these shock waves can be used for mechanically disrupting tissues and deposits.
The sum of the weight of all the atoms in a molecule.
Forceful administration under the skin of liquid medication, nutrient, or other fluid through a hollow needle piercing the skin.
The branch of surgery concerned with restoration, reconstruction, or improvement of defective, damaged, or missing structures.
Polymeric materials (usually organic) of large molecular weight which can be shaped by flow. Plastic usually refers to the final product with fillers, plasticizers, pigments, and stabilizers included (versus the resin, the homogeneous polymeric starting material). (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)
Mechanical devices that simulate the temporomandibular joints and jaws to which maxillary and mandibular casts are attached. The entire assembly attempts to reproduce the movements of the mandible and the various tooth-to-tooth relationships that accompany those movements.
Procedures used to reconstruct, restore, or improve defective, damaged, or missing structures.
Substances intended to be applied to the human body for cleansing, beautifying, promoting attractiveness, or altering the appearance without affecting the body's structure or functions. Included in this definition are skin creams, lotions, perfumes, lipsticks, fingernail polishes, eye and facial makeup preparations, permanent waves, hair colors, toothpastes, and deodorants, as well as any material intended for use as a component of a cosmetic product. (U.S. Food & Drug Administration Center for Food Safety & Applied Nutrition Office of Cosmetics Fact Sheet (web page) Feb 1995)
Tongues of skin and subcutaneous tissue, sometimes including muscle, cut away from the underlying parts but often still attached at one end. They retain their own microvasculature which is also transferred to the new site. They are often used in plastic surgery for filling a defect in a neighboring region.
Transverse sectioning and repositioning of the maxilla. There are three types: Le Fort I osteotomy for maxillary advancement or the treatment of maxillary fractures; Le Fort II osteotomy for the treatment of maxillary fractures; Le Fort III osteotomy for the treatment of maxillary fractures with fracture of one or more facial bones. Le Fort III is often used also to correct craniofacial dysostosis and related facial abnormalities. (From Dorland, 28th ed, p1203 & p662)
Thrombocytopenia occurring in the absence of toxic exposure or a disease associated with decreased platelets. It is mediated by immune mechanisms, in most cases IMMUNOGLOBULIN G autoantibodies which attach to platelets and subsequently undergo destruction by macrophages. The disease is seen in acute (affecting children) and chronic (adult) forms.
Any form of purpura in which the PLATELET COUNT is decreased. Many forms are thought to be caused by immunological mechanisms.
An acquired, congenital, or familial disorder caused by PLATELET AGGREGATION with THROMBOSIS in terminal arterioles and capillaries. Clinical features include THROMBOCYTOPENIA; HEMOLYTIC ANEMIA; AZOTEMIA; FEVER; and thrombotic microangiopathy. The classical form also includes neurological symptoms and end-organ damage, such as RENAL FAILURE.
The number of PLATELETS per unit volume in a sample of venous BLOOD.
Immunoglobulin preparations used in intravenous infusion, containing primarily IMMUNOGLOBULIN G. They are used to treat a variety of diseases associated with decreased or abnormal immunoglobulin levels including pediatric AIDS; primary HYPERGAMMAGLOBULINEMIA; SCID; CYTOMEGALOVIRUS infections in transplant recipients, LYMPHOCYTIC LEUKEMIA, CHRONIC; Kawasaki syndrome, infection in neonates, and IDIOPATHIC THROMBOCYTOPENIC PURPURA.
Molecules found on the surface of some, but not all, B-lymphocytes, T-lymphocytes, and macrophages, which recognize and combine with the Fc (crystallizable) portion of immunoglobulin molecules.
Red blood cells. Mature erythrocytes are non-nucleated, biconcave disks containing HEMOGLOBIN whose function is to transport OXYGEN.
The transference of a kidney from one human or animal to another.
An immune response with both cellular and humoral components, directed against an allogeneic transplant, whose tissue antigens are not compatible with those of the recipient.
A publication issued at stated, more or less regular, intervals.
The large fragment formed when COMPLEMENT C4 is cleaved by COMPLEMENT C1S. The membrane-bound C4b binds COMPLEMENT C2A, a SERINE PROTEASE, to form C4b2a (CLASSICAL PATHWAY C3 CONVERTASE) and subsequent C4b2a3b (CLASSICAL PATHWAY C5 CONVERTASE).
"The business or profession of the commercial production and issuance of literature" (Webster's 3d). It includes the publisher, publication processes, editing and editors. Production may be by conventional printing methods or by electronic publishing.
The use of statistical methods in the analysis of a body of literature to reveal the historical development of subject fields and patterns of authorship, publication, and use. Formerly called statistical bibliography. (from The ALA Glossary of Library and Information Science, 1983)
A contagious disease caused by canine adenovirus (ADENOVIRUSES, CANINE) infecting the LIVER, the EYE, the KIDNEY, and other organs in dogs, other canids, and bears. Symptoms include FEVER; EDEMA; VOMITING; and DIARRHEA.
Diseases of the domestic dog (Canis familiaris). This term does not include diseases of wild dogs, WOLVES; FOXES; and other Canidae for which the heading CARNIVORA is used.
Antigens determined by leukocyte loci found on chromosome 6, the major histocompatibility loci in humans. They are polypeptides or glycoproteins found on most nucleated cells and platelets, determine tissue types for transplantation, and are associated with certain diseases.
The domestic dog, Canis familiaris, comprising about 400 breeds, of the carnivore family CANIDAE. They are worldwide in distribution and live in association with people. (Walker's Mammals of the World, 5th ed, p1065)
Substances that are recognized by the immune system and induce an immune reaction.
Anomaly of the tooth, found chiefly in upper lateral incisors. It is characterized by invagination of the enamel at the incisal edge.
Critical and exhaustive investigation or experimentation, having for its aim the discovery of new facts and their correct interpretation, the revision of accepted conclusions, theories, or laws in the light of newly discovered facts, or the practical application of such new or revised conclusions, theories, or laws. (Webster, 3d ed)
A group of congenital malformations involving the brainstem, cerebellum, upper spinal cord, and surrounding bony structures. Type II is the most common, and features compression of the medulla and cerebellar tonsils into the upper cervical spinal canal and an associated MENINGOMYELOCELE. Type I features similar, but less severe malformations and is without an associated meningomyelocele. Type III has the features of type II with an additional herniation of the entire cerebellum through the bony defect involving the foramen magnum, forming an ENCEPHALOCELE. Type IV is a form a cerebellar hypoplasia. Clinical manifestations of types I-III include TORTICOLLIS; opisthotonus; HEADACHE; VERTIGO; VOCAL CORD PARALYSIS; APNEA; NYSTAGMUS, CONGENITAL; swallowing difficulties; and ATAXIA. (From Menkes, Textbook of Child Neurology, 5th ed, p261; Davis, Textbook of Neuropathology, 2nd ed, pp236-46)
A medical specialty concerned with the hypersensitivity of the individual to foreign substances and protection from the resultant infection or disorder.
An island in the Gulf of St. Lawrence constituting a province of Canada in the eastern part of the country. It is very irregular in shape with many deep inlets. Its capital is Charlottetown. Discovered by the French in 1534 and originally named Ile Saint-Jean, it was renamed in 1799 in honor of Prince Edward, fourth son of George III and future father of Queen Victoria. (From Webster's New Geographical Dictionary, 1988, p981 & Room, Brewer's Dictionary of Names, 1992, p433)
Metastatic lesion of the UMBILICUS associated with intra-abdominal neoplasms especially of the GASTROINTESTINAL TRACT or OVARY.
A disorder caused by hemizygous microdeletion of about 28 genes on chromosome 7q11.23, including the ELASTIN gene. Clinical manifestations include SUPRAVALVULAR AORTIC STENOSIS; MENTAL RETARDATION; elfin facies; impaired visuospatial constructive abilities; and transient HYPERCALCEMIA in infancy. The condition affects both sexes, with onset at birth or in early infancy.
The pit in the center of the ABDOMINAL WALL marking the point where the UMBILICAL CORD entered in the FETUS.
Time period from 1801 through 1900 of the common era.
A family of the order PRIMATES, suborder Strepsirhini (PROSIMII), containing four genera which inhabit Madagascar and the Comoro Island. Most of the lemurs prefer wooded areas. The four genera are Hapalemur, LEMUR, Lepilemur, and Varecia.
A species of the genus MACACA inhabiting India, China, and other parts of Asia. The species is used extensively in biomedical research and adapts very well to living with humans.
A phylum of fungi comprising minute intracellular PARASITES with FUNGAL SPORES of unicellular origin. It has two classes: Rudimicrosporea and MICROSPOREA.
Infections with FUNGI of the phylum MICROSPORIDIA.
A class of parasitic FUNGI. Characteristics include spores that are spherical, oval, or tubular in shape and sporoplasm which is uninuclear or binuclear.

Auto- and alloimmune reactivity to human islet allografts transplanted into type 1 diabetic patients. (1/1197)

Allogeneic islet transplantation can restore an insulin-independent state in C-peptide-negative type 1 diabetic patients. We recently reported three cases of surviving islet allografts that were implanted in type 1 diabetic patients under maintenance immune suppression for a previous kidney graft. The present study compares islet graft-specific cellular auto- and alloreactivity in peripheral blood from those three recipients and from four patients with failing islet allografts measured over a period of 6 months after portal islet implantation. The three cases that remained C-peptide-positive for >1 year exhibited no signs of alloreactivity, and their autoreactivity to islet autoantigens was only marginally increased. In contrast, rapid failure (<3 weeks) in three other cases was accompanied by increases in precursor frequencies of graft-specific alloreactive T-cells; in one of them, the alloreactivity was preceded by a sharply increased autoreactivity to several islet autoantigens. One recipient had a delayed loss of islet graft function (33 weeks); he did not exhibit signs of graft-specific alloimmunity, but developed a delayed increase in autoreactivity. The parallel between metabolic outcome of human beta-cell allografts and cellular auto- and alloreactivity in peripheral blood suggests a causal relationship. The present study therefore demonstrates that T-cell reactivities in peripheral blood can be used to monitor immune mechanisms, which influence survival of beta-cell allografts in diabetic patients.  (+info)

Antifactor VIII antibody inhibiting allogeneic but not autologous factor VIII in patients with mild hemophilia A. (2/1197)

Two unrelated patients with the same Arg2150His mutation in the factor VIII (FVIII) C1 domain, a residual FVIII activity of 0.09 IU/mL, and inhibitor titres of 300 and 6 Bethesda Units, respectively, were studied. Further analysis of patient LE, with the highest inhibitor titer, showed that (1) plasma or polyclonal IgG antibodies prepared from LE plasma inhibited the activity of allogeneic (wild-type) but not of self FVIII; (2) the presence of von Willebrand factor (vWF) increased by over 10-fold the inhibitory activity on wild-type FVIII; (3) the kinetics of FVIII inhibition followed a type II pattern, but in contrast to previously described type II inhibitors, LE IgG was potentiated by the presence of vWF instead of being in competition with it; (4) polyclonal LE IgG recognized the FVIII light chain in enzyme-linked immunosorbent assay and the recombinant A3-C1 domains in an immunoprecipitation assay, indicating that at least part of LE antibodies reacted with the FVIII domain encompassing the mutation site; and (5) LE IgG inhibited FVIII activity by decreasing the rate of FVIIIa release from vWF, but LE IgG recognized an epitope distinct from ESH8, a murine monoclonal antibody exhibiting the same property. We conclude that the present inhibitors are unique in that they clearly distinguish wild-type from self, mutated FVIII. The inhibition of wild-type FVIII by LE antibody is enhanced by vWF and is associated with an antibody-dependent reduced rate of FVIIIa release from vWF.  (+info)

Prevention of graft-versus-host disease (GVHD) by elimination of recipient-reactive donor T cells with recombinant toxins that target the interleukin 2 (IL-2) receptor. (3/1197)

Graft-versus-host disease (GVHD), due to the presence of recipient-reactive T cells, limits the usefulness of bone marrow transplantation (BMT) and is a major contributor to patient mortality. To prevent GVHD, murine and human T cells were activated by antigen or mitogens and treated with a genetically engineered form of Pseudomonas exotoxin A (PE) directed against the IL-2 receptor. Treatment with the chimeric toxin eliminated alloreactive cytotoxic T lymphocytes (CTL) as determined by cytotoxicity and mixed lymphocyte culture assays. Precursor frequencies of alloreactive cytotoxic T cells and proliferative T cells were reduced up to 100-fold as shown by limiting dilution assays. Flow cytometric analyses revealed that treatment with the chimeric toxin completely eliminated CD25+ cells from the cultures. Toxin treatment had no significant effect on hematopoietic stem and progenitor cells as determined in vitro by colony-forming assays and in vivo by long-term hematopoietic recovery after 950 rad irradiation. Toxin treatment decreased GVHD in transplanted mice to less than 10% (as compared to 88% in untreated controls). Thus, it is possible to prevent life-threatening GVHD after BMT by using a CD25 receptor-directed toxin to eliminate host-reactive T cells from bone marrow grafts.  (+info)

The role of the human Fc receptor Fc gamma RIIA in the immune clearance of platelets: a transgenic mouse model. (4/1197)

In humans, the Fc receptor for IgG, FcgammaRIIA, is expressed on macrophages and platelets and may play an important role in the pathophysiology of immune-mediated thrombocytopenia. Mice lack the genetic equivalent of human FcgammaRIIA. To better understand the role of FcgammaRIIA in vivo, FcgammaRIIA transgenic mice were generated and characterized. One transgenic mouse line expressed FcgammaRIIA on platelets and macrophages at levels equivalent to human cells, and cross-linking FcgammaRIIA on these platelets induced platelet aggregation. Immune-mediated thrombocytopenia in this transgenic line was studied using i.v. and i.p. administration of anti-mouse platelet Ab. In comparison with matched wild-type littermates that are negative for the FcgammaRIIA transgene, Ab-mediated thrombocytopenia was significantly more severe in the FcgammaRIIA transgenic mice. In contrast, FcR gamma-chain knockout mice that lack functional expression of the Fc receptors FcgammaRI and FcgammaRIII on splenic macrophages did not demonstrate Ab-mediated thrombocytopenia. We generated FcgammaRIIA transgenic x FcR gamma-chain knockout mice to examine the role of FcgammaRIIA in immune clearance in the absence of functional FcgammaRI and FcgammaRIII. In FcgammaRIIA transgenic x FcR gamma-chain knockout mice, severe immune thrombocytopenia mediated by FcgammaRIIA was observed. These results demonstrate that FcgammaRIIA does not require the FcR gamma-chain for expression or function in vivo. Furthermore, taken together, the data suggest that the human Fc receptor FcgammaRIIA plays a significant role in the immune clearance of platelets in vivo.  (+info)

Marked mitigation of transplant vascular sclerosis in FasLgld (CD95L) mutant recipients. The role of alloantibodies in the development of chronic rejection. (5/1197)

BACKGROUND: In the acute rejection of allografts, the interaction between Fas (CD95) and its ligand (FasL; CD95L) has been shown to be involved in mediating apoptotic cell death. The role, however, of these molecules in the pathogenesis of transplant vascular sclerosis is as yet undetermined. The present study was therefore designed to address this issue. MATERIAL: C3H/HEJ FasLgld (FasL-; H2k) spontaneously mutant mice were used either as donors or recipients of aortic allografts; wild-type C57B1/6 (B6; H2b) were used as corresponding recipients or donors (n=6/group), respectively. Controls included aortas transplanted across appropriate allogeneic and syngeneic strain combinations. For histopathological evaluations, the grafts were harvested at day 40 after transplantation, at which time, splenocytes and sera were also obtained for mixed leukocyte reaction and complement-mediated microcytotoxicity assays, respectively. RESULTS: Similar to aortas obtained from allogeneic controls, allografts harvested from FasL- -->B6 recipients had morphological evidence of chronic rejection characterized by circumferential intimal thickening with partial disruption of the elastic membranes. Correspondingly, heightened antidonor cellular reactivity was also witnessed in these recipients. On the contrary, B6 allografts harvested from the majority of C3H-->FasL- recipients exhibited marked preservation of aortic morphology. Although these recipients had diminished antidonor cellular proliferation, the titers of alloantibodies were markedly elevated. CONCLUSION: The presence of FasL-expressing functional cytotoxic T cells is required for the pathogenesis of transplant vascular sclerosis. The significant reduction and/or absence of chronic rejection with the concomitant retention of antidonor humoral response in C3H FasL- recipients of B6 aortas prompt us to suggest that perhaps posttransplantation vasculopathy is initiated by cell-mediated cytotoxicity with its perpetuation facilitated by alloantibodies.  (+info)

Rapamycin reverses chronic graft vascular disease in a novel cardiac allograft model. (6/1197)

BACKGROUND: Chronic graft vascular disease (CGVD) in cardiac allografts has been defined as a slowly evolving vasculopathy unresponsive to conventional immunosuppression. We compared 4 rodent models of CGVD to evaluate the reproducibility of CGVD in heart allografts. Rapamycin (Rapa) and cyclosporine (CSA) were then used to treat CGVD. METHODS AND RESULTS: Hearts were harvested and placed heterotopically into allogenic recipients. CGVD scores of PVG allografts from ACI recipients treated with CSA on days 1 through 10 were significantly elevated on day 90 (n=16) compared with other models (immunosuppression used): (1) Lewis to F344 recipients (CSA), (2) Brown Norway to Lewis (FK506), and (3) DA to Wistar-Firth (methylprednisolone, azathioprine, CSA). Although delayed (day 60 to 90) CSA treatment had no effect (n=6), delayed Rapa (3 mg. kg-1. d-1 IP) reversed CGVD in PVG grafts (0.22+/-0.19 on day 90, n=6). ACI isografts showed no evidence of CGVD (n=6) at day 90. Immunohistochemistry of PVG grafts revealed perivascular infiltrates consisting of CD4(+) T cells and limited numbers of macrophages persisting up to day 90. Flow cytometry demonstrated increased levels of anti-donor antibody at day 90, which was significantly inhibited by Rapa treatment. CONCLUSIONS: PVG grafts developed a significant increase in CGVD without evidence of ongoing myocardial rejection. This CGVD appeared to be mediated by both cellular and humoral mechanisms, given CD4(+) perivascular infiltrates and increased levels of anti-donor antibody. The anti-CGVD effectiveness of Rapa during a period in which there was little myocardial cellular infiltrate supports a novel mechanism of effect such as smooth muscle or B-cell inhibition.  (+info)

Alloantibody-mediated class I signal transduction in endothelial cells and smooth muscle cells: enhancement by IFN-gamma and TNF-alpha. (7/1197)

Chronic rejection is the major limiting factor to long term survival of solid organ allografts. The hallmark of chronic rejection is transplant atherosclerosis, which is characterized by the intimal proliferation of smooth muscle cells, endothelial cells, and fibroblasts, leading to vessel obstruction, fibrosis, and eventual graft loss. The mechanism of chronic rejection is poorly understood, but it is suspected that the associated vascular changes are a result of anti-HLA Ab-mediated injury to the endothelium and smooth muscle of the graft. In this study we have investigated whether anti-HLA Abs, developed by transplant recipients following transplantation, are capable of transducing signals via HLA class I molecules, which stimulate cell proliferation. In this report we show that ligation of class I molecules with Abs to distinct HLA-A locus and HLA-B locus molecules results in increased tyrosine phosphorylation of intracellular proteins and induction of fibroblast growth factor receptor expression on endothelial and smooth muscle cells. Treatment of cells with IFN-gamma and TNF-alpha up-regulated MHC class I expression and potentiated anti-HLA Ab-induced fibroblast growth factor receptor expression. Engagement of class I molecules also stimulated enhanced proliferative responses to basic fibroblast growth factor, which augmented endothelial cell proliferation. These findings support a role for anti-HLA Abs and cytokines in the transduction of proliferative signals, which stimulate the development of myointimal hyperplasia associated with chronic rejection of human allografts.  (+info)

Mixed chimerism induced without lethal conditioning prevents T cell- and anti-Gal alpha 1,3Gal-mediated graft rejection. (8/1197)

Gal alpha 1,3Gal-reactive (Gal-reactive) antibodies are a major impediment to pig-to-human xenotransplantation. We investigated the potential to induce tolerance of anti-Gal-producing cells and prevent rejection of vascularized grafts in the combination of alpha 1,3-galactosyltransferase wild-type (GalT(+/+)) and deficient (GalT(-/-)) mice. Allogeneic (H-2 mismatched) GalT(+/+) bone marrow transplantation (BMT) to GalT(-/-) mice conditioned with a nonmyeloablative regimen, consisting of depleting CD4 and CD8 mAb's and 3 Gy whole-body irradiation and 7 Gy thymic irradiation, led to lasting multilineage H-2(bxd) GalT(+/+) + H-2(d) GalT(-/-) mixed chimerism. Induction of mixed chimerism was associated with a rapid reduction of serum anti-Gal naturally occurring antibody levels. Anti-Gal-producing cells were undetectable by 2 weeks after BMT, suggesting that anti-Gal-producing cells preexisting at the time of BMT are rapidly tolerized. Even after immunization with Gal-bearing xenogeneic cells, mixed chimeras were devoid of anti-Gal-producing cells and permanently accepted donor-type GalT(+/+) heart grafts (>150 days), whereas non-BMT control animals rejected these hearts within 1-7 days. B cells bearing receptors for Gal were completely absent from the spleens of mixed chimeras, suggesting that clonal deletion and/or receptor editing may maintain B-cell tolerance to Gal. These findings demonstrate the principle that induction of mixed hematopoietic chimerism with a potentially relevant nonmyeloablative regimen can simultaneously lead to tolerance among both T cells and Gal-reactive B cells, thus preventing vascularized xenograft rejection.  (+info)

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While DHTR can be life-threatening, unawareness of their frequency and lack of severity of these transfusion reactions have likely resulted in too little attention regarding their potential impact on overall SCD morbidity and mortality. For years, the overall incidence of DHTR per transfusion in SCD was estimated to be around 1:1000.22-24 making it a relatively uncommon transfusion reaction in this population of patients. However, newer reports suggest that these data may be misleading. As mentioned previously, given the similarities between the clinical presentation of DHTR and the more common complications of vaso-occlusive crises, DHTR can be easily missed.6,7,25 Unless an alloantibody screen is performed, an amnestic alloantibody response will not be detected and a diagnosis of DHTR may not be entertained. Furthermore, as some reports suggest that as many as 30% of DHTR can be alloantibody-negative,14,26 clinicians must rely on HbA measurements obtained within 48 h of the implicated ...
Lutton BV, Cho PS, Hirsh EL, Ferguson KK, Teague AG, Hanekamp JS, Chi N, Goldman SN, Messina DJ, Houser S, Yeap BY, Popma SH, Sachs DH, Huang CA. Approaches to avoid immune responses induced by repeated subcutaneous injections of allogeneic umbilical cord tissue-derived cells. Transplantation. 2010 Sep 15; 90(5):494-501 ...
Effects of maternal serum IgG anti-A (B) and neonatal direct anti globulin red blood cell antibody expression on the occurrence and development of neonatal ABO haemolysis, Ming Che
Allograft rejection is the consequence of the recipients alloimmune response to nonself antigens expressed by donor tissues. After transplantation of organ allografts, there are two pathways of antigen presentation. In the direct pathway, recipient T cells react to intact allogeneic MHC molecules expressed on the surface of donor cells. This pathway would activate host CD4 or CD8 T cells. In contrast, donor MHC molecules (and all other proteins) shed from the graft can be taken up by host APCs and presented to recipient T cells in the context of self-MHC molecules - the indirect pathway. Such presentation activates predominantly CD4 T cells. A direct cytotoxic T-cell attack on graft cells can be made only by T cells that recognize the graft MHC molecules directly. Nontheless, T cells with indirect allospecificity can contribute to graft rejection by activating macrophages, which cause tissue injury and fibrosis, and are also likely to be important in the development of an alloantibody response ...
CD154 (CD40 ligand, gp39) interaction with its receptor CD40 has been shown to be critically important for the generation of cell-mediated as well as humoral immunity. It has been proposed that ligation of CD40 on APCs, presumably by activated Th cells, leads to increased APC function as defined by up-regulation of costimulatory molecules and enhancement of IL-12 production. In this report, we directly examined the contribution of the CD154:CD40 pathway in a murine model of allograft rejection. Generation of both the CTL and alloantibody responses following injection with allogeneic P815 tumor cells was severely compromised in CD154 knockout mice and wild-type C57BL/6 mice treated with the anti-CD154 mAb, MR1. Splenic production of IL-2, IFN-γ, and TNF was significantly suppressed from CD154-deficient mice, indicating a lack of T cell priming. However, splenic cells from CD154 knockout mice induced comparable levels of CD86 expression and IL-12 production when compared with their wild-type ...
HLA donor specific antibody (DSA) mediates injury by both complement dependent and independent mechanisms, including direct signaling via HLA antigens and via Fc receptor (FcR) engagement. Functional assessment of DSA has historically been limited to complement-based assays. FcR binding capacity and IgG isotypes provide complementary approaches for assessment of pathogenic potential of DSA. Assessment of the effects of proteasome inhibitor (PI) based treatment on global immunodominant DSA (iDSA) functional capacity has not been seen previously. We present analysis of comprehensive iDSA functional capacity assessment following PI-based treatment.. Methods: Serum samples obtained at the time of AMR diagnosis and following AMR treatment were analyzed by HLA single antigen bead (SAB) microarrays, C1q assay, IgG isotype-specific SAB assays (IgG1, IgG2, IgG3, IgG4), and FcR binding assays (performed per standard SOPs for the laboratory-developed test). iDSA FcR binding strength and incidence of IgG3 ...
Approximately 3-10% of human red blood cell (RBC) transfusion recipients form alloantibodies to non-self, non-ABO blood group antigens expressed on donor RBCs, with these alloantibodies having the potential to be clinically significant in transfusion and pregnancy settings. However, the majority of transfused individuals never form detectable alloantibodies. Expanding upon observations that children initially transfused with RBCs at a young age are less likely to form alloantibodies throughout their lives, we hypothesized that non-responders may not only be ignorant of antigens on RBCs but instead tolerized. We investigated this question in a reductionist murine model, in which transgenic donors express the human glycophorin A (hGPA) antigen in an RBC-specific manner. Although wild-type mice treated with poly IC and transfused with hGPA RBCs generated robust anti-hGPA IgG alloantibodies that led to rapid clearance of incompatible RBCs, those transfused in the absence of an adjuvant failed to ...
The current half-life of a transplanted organ has not improved in a very long time. Historical reports on the causes of allograft failure have pointed to a plethora of contributing issues. However, in recent years, alloantibody-mediated injury has emerged as the major cause of allograft loss. Alloantibodies and the alloimmune response have always been a cause of allograft failure. Early failure and hyperacute rejection were a direct result of the alloimmune response. Fortunately, the use of crossmatch techniques and avoiding crossmatch positive transplants has largely eliminated early alloimmune rejections. However, it is also now clear that patients can develop antibody-mediated injury in the first year post-transplant and beyond ...
Searching for mechanisms of natural resistance to HIV infection with which to guide HIV vaccine design, we have examined antibody responses to HLA class I antigens in children of HIV-1-infected mothers. Anti-HLA antibodies are known to block HIV infectivity in vitro and can be protective against SIV challenge in macaques immunized with purified class I HLA. It was hypothesized that alloantibody to maternal HLA in children might contribute to the prevention of mother-to-child transmission of HIV-1. In fact, a surprisingly high proportion of the children examined, 22%, were found to have antibody against class I alloantigens. This alloantibody, however, did not correlate with the HIV status of the children and was found in a similar proportion of children of HIV-negative mothers. The HLA specificity of the antibody was not correlated with noninherited maternal HLA alleles and occurred with a higher frequency in older children. This result suggests environmental factors, rather than exposure to ...
Greek: different, other, another; divergence; a combining form denoting a condition differing from the normal or a reversal, or referring to another
Allograft rejection is the consequence of the recipients alloimmune response to nonself antigens expressed by donor tissues. After transplantation of organ allografts, there are two pathways of antigen presentation. In the direct pathway, recipient T cells react to intact allogeneic MHC molecules expressed on the surface of donor cells. This pathway would activate host CD4 or CD8 T cells. In contrast, donor MHC molecules (and all other proteins) shed from the graft can be taken up by host APCs and presented to recipient T cells in the context of self-MHC molecules - the indirect pathway. Such presentation activates predominantly CD4 T cells. A direct cytotoxic T-cell attack on graft cells can be made only by T cells that recognize the graft MHC molecules directly. Nontheless, T cells with indirect allospecificity can contribute to graft rejection by activating macrophages, which cause tissue injury and fibrosis, and are also likely to be important in the development of an alloantibody response ...
Introduction. Complement activating donor specific antibodies may be associated with worse outcomes in a cohort of patients who have undergone HLA-AIT. We looked for differences in renal allograft outcomes in patients with HLA class 1 and class 2 complement activating IgG antibodies.. Methods: We analysed samples from 121 HLA-AIT who had pre-transplant DSA and subsequently underwent direct transplantation.C3d (Immucor) assay was performed at preconditioning/pre-transplant. IgG C3d results were correlated with HLA class and for prediction of rejection and allograft survival.. Results: Of 121 patients,11 had C3d positive IgG DSA for class1 and 31 had C3d positive IgG DSA positive for class 2. 5 patients had C3d IgG DSA positive for both classes. Either class 1 or class 2 C3d positive DSA was not associated with early rejection (p 0..64 and p0.146 respectively). C3d class 1 DSA was not associated with predicting long term graft survival (p 0.1) ...
As a result of the advances in veterinary medicine the use and availability of blood products has dramatically increased. Understanding the genetics and role of blood types in dogs and cats is important for veterinarians, breeders, and pet owners to appreciate, because of the impact blood type incompatibilities can have. Blood typing, in any species, is not only necessary to improve the safety and efficacy of transfusions, but the knowledge it provides can prevent fatal neonatal isoerythrolysis during the first days of life in cats. Methods for blood typing and crossmatching have become available for use in practice and in the laboratory. BLOOD TYPES. Blood types refer to genetic markers on the surface of a red blood cell (erythrocyte) that are both species-specific and antigenic. Antigenicity refers to the likelihood the immune system will react and make antibodies, known as alloantibodies or isoantibodies, against the foreign substance; these antibodies can be detected in the plasma. A set of ...
TY - JOUR. T1 - Frequencies and specificities of red cell alloantibodies in the Southern Thai population. AU - Promwong, Charuporn. AU - Siammai, Santi. AU - Hassarin, Sarunya. AU - Buakaew, Jarin. AU - Yeela, Tanongsak. AU - Soisangwan, Patravee. AU - Roxby, David. PY - 2013/1. Y1 - 2013/1. N2 - Context: Detailed reports of red cell alloantibody frequencies and specificities in the Thai population are limited. The aims of this study were to determine the specificity and compare the frequency of alloantibodies detected using column agglutination technology (CAT) and conventional tube techniques in blood donors and previously transfused patients. Settings and Design: We retrospectively reviewed antibody screening and identification records for two time periods: January-December 2006 during which conventional tube techniques were used and January 2008-December 2009 when CAT was used. Results: The overall prevalence of alloantibodies in both patients and donors when using conventional tube ...
BACKGROUND: Antibodies to Mia , MUT, and Mur are among the most frequently identified alloantibodies in Southeast Asia. Understanding the characteristics of these antibodies in terms of induction and evanescence would aid in optimizing methods for their detection. STUDY DESIGN AND METHODS: Antibody testing results between the years 2013 and 2015 with relevant patient demographic data and red blood cell (RBC) transfusion history were retrieved. Cumulative alloimmunization incidence and evanescence to MUT and Mur were estimated by Kaplan-Meier analysis in relation to the number of RBC units transfused and time. RESULTS: Of 70,543 selected patients, 6186 nonalloimmunized subjects with available antibody testing results posttransfusion were identified. Cumulative alloimmunization incidence for MUT increased from 0.12% (95% confidence interval [CI], 0.03-0.21) to 0.63% (95% CI, 0.25-1.01), while for Mur it increased from 0.04% (95% CI, 0-0.09) to 0.42% (95% CI, 0.05-0.79) when a patient was ...
This trial is assessing the efficacy, safety and tolerability of a desensitisation protocol of rituximab + intravenous immune globulin (IVIG) in highly-HLA
Alice Koenig, Sarah Mezaache, Jasper Callemeyn, Thomas Barba, Virginie Mathias, Antoine Sicard, Béatrice Charreau, Maud Rabeyrin, Frédérique Dijoud, Cécile Picard, Vannary Meas-Yedid, Jean-Christophe Olivo-Marin, Emmanuel Morelon, Maarten Naesens, Valérie Dubois and Olivier Thaunat ...
BACKGROUND: The D antigen of the polymorphic Rh blood group system is of particular clinical importance regarding transfusion- and pregnancy-induced alloimmunization. Different RhD variants with specific clinical implications have been characterized. The least expressed D variants collectively called DEL are serologically detectable only by adsorption-elution techniques, with so far only poorly defined antigenic properties.. STUDY DESIGN AND METHODS: A comprehensive immunohematologic analysis of five of the six currently known DEL genotypes was performed. DEL phenotypes associated with the RHD(M295I), RHD(IVS3+1g,a), RHD(K409K), RHD(X418L), or RHD(IVS5-38del4) allele were characterized with extended serology and flow cytometry.. RESULTS: Epitope mapping with adsorption-elution revealed a prominent D epitope loss in the RHD(IVS3+1g,a)-associated DEL phenotype, whereas in the other four DEL types no signs of qualitative D antigen alteration were detected. The observation of alloanti-D in two ...
An IgG-specific red cell antibody detection, identification and crossmatch system. Capture-R® Ready-Screen® technology is utilized in the detection of unexpected IgG antibodies to red blood cells by manual, semi-automated or fully automated test methods. Immucors broad menu of Capture-R red cell antibody screen and identification assays provides flexibility and standardization in pre-transfusion testing ...
Standardized DSA testing and diagnostic criteria for both acute and chronic AMR are needed to distil existing associations into etiological processes in order to develop responsive therapeutic strategies.
MICA兔多克隆抗体(ab93170)可与人样本反应并经WB, IHC, Flow Cyt实验严格验证。中国75%以上现货,所有产品均提供质保服务,可通过电话、电邮或微信获得本地专属技术支持。
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g d antigens, b d antigens, n d antigens, j d antigens, u d antigens, y d antigens, h s antigens, h x antigens, h c antigens, h f antigens, h r antigens, h e antigens, h d zntigens, h d sntigens, h d wntigens, h d qntigens, h d abtigens, h d amtigens, h d ajtigens, h d ahtigens, h d anrigens, h d anfigens, h d angigens, h d anyigens, h d an6igens, h d an5igens, h d antugens, h d antjgens, h d antkgens, h d antogens, h d ant9gens, h d ant8gens, h d antifens, h d antivens, h d antibens, h d antihens, h d antiyens, h d antitens, h d antigwns, h d antigsns, h d antigdns, h d antigrns, h d antig4ns, h d antig3ns, h d antigebs, h d antigems, h d antigejs, h d antigehs, h d antigena, h d antigenz, h d antigenx, h d antigend, h d antigene, h d antigenw, gh d antigens, hg d antigens, bh d antigens, hb d antigens, nh d antigens, hn d antigens, jh d antigens, hj d antigens, uh d antigens, hu d antigens, yh d antigens, hy d antigens, h sd antigens, h ds antigens, h xd antigens, h dx antigens, h cd antigens, ...
Cases of CD36 deficiency are not rare in Asian populations, foetal and neonatal alloimmune thrombocytopenia (FNAIT) caused by anti-CD36 isoantibodies appears more frequent than other HPA...
BACKGROUND: The traditional method for assessing HLA antibodies in recipient serum samples is the complement-dependent cytotoxicity testing (CDC). Recently, the highly sensitive microbead-based Luminex assay was introduced and can detect low levels of anti-HLA Abs.. OBJECTIVE: To determine the impact of pretransplant donor-specific HLA antibodies (DSA) detectable by Luminex, despite a negative CDC crossmatch, on the outcomes of kidney transplantation. The correlation and cut-off value of panel reactive antibody (PRA) and DSA was also evaluated.. METHODS: Pre-transplant sera from 116 kidney transplant recipients with a negative CDC crossmatch were assessed for donor-specific HLA antibodies by using Luminex single antigen beads. The patients received kidney transplants at Ramathibodi Hospital between January 2003 and December 2007. The results were correlated with kidney graft outcomes.. RESULTS: DSA were found in 24.1% (28/116) of all recipients. Of the twenty-eight DSA positive patients, four ...
Occurrence of anti-D alloantibodies among pregnant women in Kasese District, Western Uganda Yona Mbalibulha,1 Enoch Muwanguzi,1 Godfrey R Mugyenyi,2 Bernard Natukunda1 1Department of Medical Laboratory Sciences, 2Department of Obstetrics and Gynecology, Faculty of Medicine, Mbarara University of Science and Technology, Mbarara, Uganda Objectives: This study was undertaken to determine the distribution of ABO/RhD (rhesus D antigen) blood phenotypes, prevalence of anti-D alloantibodies, and the risk factors for alloimmunization among pregnant women in Kasese District, Western Uganda. Materials and methods: Ethylenediamine tetraacetic acid-containing plasma samples and serum samples were taken from pregnant women attending the antenatal clinic. The blood groups were identified using the microplate grouping method, while the presence of anti-D alloantibodies was detected by the indirect antiglobulin test (IAT). Data were also collected from the pregnant women on the risk factors associated with anti-D
Looking for Antibody-Mediated Rejection? Find out information about Antibody-Mediated Rejection. Destruction of a graft by the immune system of the recipient. Rejection in a dream may suggest that there are feelings or situations the dreamer wants to be... Explanation of Antibody-Mediated Rejection
Introduction: The development of de novo donor-specific antibodies (dnDSA) has been associated with rejection and graft loss in kidney transplantation, and DSA screening is now recommended in all kidney transplant recipients. However, the clinical significance of dnDSA in patients with a stable creatinine remains unclear. Methods: We performed a retrospective cohort study of 103 patients receiving a first, kidney alone transplant between 12/1/2007 and 12/31/2013. Inclusion criteria were age ,18 years old at the time of transplant and at least two years of DSA monitoring. All patients underwent DSA screening every 3 months post-transplant with additional testing as clinically indicated. No treatment was given for DSAs in the absence of biopsy-proven rejection. Results: 20 patients (19%) developed dnDSA in the setting of a stable creatinine and 13 patients (13%) developed dnDSA in the setting of an elevated creatinine. Median follow-up time post-transplant was 4.1 (IQR 2.9-5.7) years. In a Cox ...
Individuals that become immunized to red blood cell (RBC) alloantigens can experience an increased rate of antibody formation to additional RBC alloantigens following subsequent transfusion. Despite this, how an immune response to one RBC immunogen may impact subsequent alloimmunization to a completely different RBC alloantigen remains unknown. Our studies demonstrate that Kell blood group antigen (KEL) RBC transfusion in the presence of inflammation induced by poly (I:C) (PIC) not only enhances anti-KEL antibody production through a CD4+ T-cell-dependent process but also directly facilitates anti-HOD antibody formation following subsequent exposure to the disparate HOD (hen egg lysozyme, ovalbumin, fused to human blood group antigen Duffy b) antigen ...
TY - JOUR. T1 - Personalized peptide arrays for detection of HLA alloantibodies in organ transplantation. AU - Liu, Pan. AU - Souma, Tomokazu. AU - Wei, Andrew Zu Sern. AU - Xie, Xueying. AU - Luo, Xunrong. AU - Jin, Jing. PY - 2017/9/6. Y1 - 2017/9/6. N2 - In organ transplantation, the function and longevity of the graft critically rely on the success of controlling immunological rejection reactivity against human leukocyte antigens (HLA). Histocompatibility guidelines are based on laboratory tests of anti-HLA immunity, which presents either as pre-existing or de novo generated HLA antibodies that constitute a major transplantation barrier. Current tests are built on a single-antigen beads (SAB) platform using a fixed set of ~100 preselected recombinant HLA antigens to probe transplant sera. However, in humans there exist a far greater variety of HLA types, with no two individuals other than identical twins who can share the same combination of HLA sequences. While advanced technologies for HLA ...
Factor VIII antibody [RFF-VIIIC/8] (coagulation factor VIII) for ELISA, RIA, WB. Anti-Factor VIII mAb (GTX41177) is tested in Human, Pig samples. 100% Ab-Assurance.
Summary A modified cytotoxicity assay was adapted from classical erythrocytolytic assays, in which complement components were added in sequence to antibody-sensitized cells. This assay was applied to a model system in which mouse sarcoma cells were sensitized with H-2 alloantibody. The stepwise pr...
Low-ionic-strength saline (LISS) techniques permit a safe and substantial reduction in incubation time and have therefore become the method of choice for antibody detection and compatibility testing in many transfusion laboratories. Consequently, the supply of reagent red cells (RBCs) in a low-ionic-strength preservative solution would remove the daily need for laboratories to wash and resuspend cells in LISS before use. However, the storage of fresh RBCs at low ionic strength in the presence of aminoglycoside antibiotics can cause a rapid loss of certain antigens, possibly as a result of the release of proteolytic enzymes from contaminating white cells. This article describes a low-ionic-strength solution that achieves preservation of antigens on liquid nitrogen-frozen-thawed RBCs for 21 days storage at 4°C. ...
Unlike the relatively simple human ABO system, the horse has 7 systems, each of which contain a number of specific factors. Because of the variety of possible combinations, red cell typing is very informative for parentage determination. For each factor or red cell antigen that we test for, we must develop our own specific reagent or antibody to detect that factor. Our panel of reagents is screened exhaustively for specificity and reliability in our tests.. Biochemical Polymorphisms ...
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C1Q Single Antigen Antibody Class I Order Code: C1QA1 Reference Laboratory: UCLA Reference Laboratory
The molecular basis of the HNA-3a/b (5b/a) leukocyte antigen system has not yet been defined despite evidence that HNA-3a… Expand ...
يهيمن المجلس على قواعد الدراسة والامتحانات والمعادلات والممارسة وشهادة اتمام التدريب الاساسى (لا توجد فىمصر حتى الان) ونقاط التنمية المهنية المستدامة ويسجل الاطباء بناء على مؤهل وبرنامج تدريب قومى ويحاسبهم. ينشأ المجلس كليات قومية تجرى امتحانات قومية أيا كان مكان عمل الطبيب وتنميه مهنيا بالتعاون مع الجمعيات العلمية المسجلة (اقترح اعادة تسجيلها نظرا لعددها الحالي غير الواقعي والصراعات بينها) بدلا من تأرجح الطبيب بين شهادات جامعية متفاوتة المستوى فى انحاء مصر وبين قبول تسجيله فى جامعة ما وفيما يطلق عليه زمالة وزارة الصحة التي تطبق علي البعض فقط ...
Background. Maternal alloantibodies against HPA-1a can cross placenta, opsonize foetal platelets, and induce neonatal alloimmune thrombocytopenia (NAIT). In a study of 100, 448 pregnant women in Norway during 1995-2004, 10.6% of HPA-1a negative women had detectable anti-HPA-1a antibodies. Design and Methods. A possible correlation between the maternal ABO blood group phenotype, or underlying genotype, and severe thrombocytopenia in the newborn was investigated. Results. We observed that immunized women with blood group O had a lower risk of having a child with severe NAIT than women with group A; 20% with blood group O gave birth to children with severe NAIT, compared to 47% among the blood group A mothers (relative risk 0.43; 95% CI 0.25-0.75). Conclusion. The risk of severe neonatal alloimmune thrombocytopenia due to anti-HPA-1a antibodies is correlated to maternal ABO types, and this study indicates that the observation is due to genetic properties on the maternal side ...
TY - JOUR. T1 - Histopathology and immunophenotype of the spleen during acute antibody-mediated rejection. T2 - Case report. AU - Kaplan, B.. AU - Jie, T.. AU - Diana, R.. AU - Renz, J.. AU - Whinery, A.. AU - Stubbs, N.. AU - Bracamonte, E.. AU - Spier, C.. AU - Schubart, P.. AU - Rilo, H.. AU - Gruessner, R.. N1 - Copyright: Copyright 2010 Elsevier B.V., All rights reserved.. PY - 2010/5. Y1 - 2010/5. N2 - Splenectomy has been reported to have a beneficial effect in treating Acute antibody-mediated rejection (ABMR). This reason for this often rapid and profound beneficial effect is not readily apparent from what is known about normal splenic immunoarchitecture. While the spleen is rich in mature B cells, it has not been noted to be a repository for direct antibody-secreting cells. We present a case of a Native American female who received a renal transplant and developed a severe episode of ABMR. The patient was initially refractory to both plasmapheresis and IVIG. The patient underwent an ...
Today we will be discussing fetal and neonatal alloimmune thrombocytopenia, also referred to as NAIT, which affects about 1 in 1,000 births. NAIT is caused by maternal alloantibodies to specific platelet antigens, usually human platelet antigen 1a also referred to as HPA-1a. These antigens may be inherited from the father but are absent in the mother. Only 2% of women are HPA-1a negative and at risk to produce these alloantibodies which can lead to fetal thrombocytopenia.. Dr. Bussel, who wrote an Editorial on the subject, discusses the morbidity and mortality associated with NAIT.. If the platelets are low enough, there is a serious risk of bleeding. When the fetal platelet get low enough, then the fetus may bleed in the brain and most of the bleeding in the brain that happens in fetuses and neonates affected by NAIT, occurs in utero, not after birth.. Currently, there are no screening guidelines to identify mothers and fetuses that are at risk for NAIT. Therefore, it is usually diagnosed ...
TY - JOUR. T1 - The Value of Protocol Biopsies to Identify Patients With De Novo Donor-Specific Antibody at High Risk for Allograft Loss. AU - Schinstock, Carrie. AU - Cosio, Fernando G. AU - Cheungpasitporn, W.. AU - Dadhania, D. M.. AU - Everly, M. J.. AU - Samaniego-Picota, M. D.. AU - Cornell, L.. AU - Stegall, Mark D. PY - 2017/6/1. Y1 - 2017/6/1. N2 - De novo donor-specific antibody (dnDSA) is associated with antibody-mediated rejection (AMR) and allograft loss, yet the allograft histology associated with dnDSA remains unclear. The aim of this study was to examine the allograft histology associated with dnDSA in patients with serial surveillance biopsies. We retrospectively studied adult conventional solitary kidney transplant recipients from October 2007 to May 2014. The definition of dnDSA was new donor-specific antibody (DSA) with mean fluorescence intensity (MFI) ,1000. The incidence of dnDSA was 7.0% (54 of 771) over mean follow-up of 4.2 ± 1.9 years. Patients with dnDSA had reduced ...
The study shows that transfusion of blood matched for Rh and Kell systems would reduce alloimmunization to 27% and reduce delayed hemolytic transfusion reaction, but would not prevent it. Delayed hemolytic transfusion reactions were mild and did not cause hyperhemolysis in sickle cell patients. However, the cause of hyperhemolysis, which is a severe problem, was neither due to allo- nor autoimmunization, and could not be prevented by matching for RBC antigens. Hyperhemolysis may be triggered by transfusion, but its cause seems to be multifactorial. Therefore, the authors conclude that the use of costly extended antigen-matched RBCs is not necessary. Unfortunately, the authors give no details of the further investigation of the autoantibodies (eluates, etc.) in order to be sure that no alloantibodies were imitating them. Further studies should use flow cytometry in order to follow up the survival of antigen-matched and -unmatched allogeneic RBCs as well as autologous RBCs.. - V. Kretschmer. ...
RhD negative pregnant women who carry an RhD positive fetus are at risk of immunization against the D antigen, which may result in hemolytic disease of the fetus and the newborn. Predicting the fetal RhD status by noninvasive antenatal screening for the fetal RhD gene (RHD) can guide targeted use of antenatal anti-D prophylaxis.Cell-free fetal DNA is extracted from maternal plasma from RhD negative pregnant women at a gestational age of 25 weeks. A real-time PCR-based detection of two RHD exons enables reliable prediction of the fetal RhD status to determine the administration of antenatal prophylaxis, as well as postnatal prophylaxis. ...
Current Research and Scholarly Interests From 2005 to 2010, my work as a clinical hematology fellow allowed me to experience first-hand how scientific advances that started in a laboratory can transform the lives of patients. While many of my patients were cured of their disease with allogeneic hematopoietic stem cell transplantation, underscoring the importance of anti-tumor immunotherapy in eradicating leukemia, I witnessed face-to-face their suffering from the long-term consequence of graft-versus-host disease (GVHD). This experience was ultimately what drove me to engage in research to discover novel therapies. For this reason, I embarked on a PhD program in 2010 to design antibody therapy to (i) target GVHD and (ii) target hematological malignancies. Under the mentorship of Professor Hiromitsu Nakauchi at the University of Tokyo, an international leader in hematopoiesis, I developed allele-specific anti-human leukocyte antigen (HLA) monoclonal antibodies for severe GVHD caused by ...
Qian J, Collins M, Sharpe AH, Hoyer LW. Prevention and treatment of factor VIII inhibitors in murine hemophilia A. Blood. 2000;95 (4) :1324-9.
Alloimmunity (sometimes called isoimmunity) is an immune response to nonself antigens from members of the same species, which are called alloantigens or isoantigens. Two major types of alloantigens are blood group antigens and histocompatibility antigens. In alloimmunity, the body creates antibodies against the alloantigens, attacking transfused blood, allotransplanted tissue, and even the fetus in some cases. Alloimmune (isoimmune) response results in graft rejection, which is manifested as deterioration or complete loss of graft function. In contrast, autoimmunity is an immune response to the selfs own antigens. (The allo- prefix means other, whereas the auto- prefix means self.) Alloimmunization (isoimmunization) is the process of becoming alloimmune, that is, developing the relevant antibodies for the first time. Alloimmunity is caused by the difference between products of highly polymorphic genes, primarily genes of the major histocompatibility complex, of the donor and graft ...
International Society of Blood Transfusion (ISBT) Working Party has organized red cell antigens into 25 blood group systems. Red cell antigens that ar...
Purpose To determine the impact of transplant nephrectomy on morbidity and mortality and HLA immunization. Methods All patients who underwent transplant nephrectomy in our centre between 2000 and 2016 were included in this study. A total of 2822 renal transplantations and 180 transplant nephrectomies were performed during this period. Results The indications for transplant nephrectomy were graft intolerance syndrome: 47.2%, sepsis: 22.2%, vascular thrombosis: 15.5%, tumour: 8.3% and other 6.8%. Transplant nephrectomies were performed via an intracapsular approach in 61.7% of cases. The blood transfusion rate was 50%, the morbidity rate was 38% and the mortality rate was 3%. Transplant nephrectomies more than 12 months after renal transplant failure were associated with more complications (p = 0.006). Transfusions in the context of transplant nephrectomy had no significant impact on alloimmunization. Conclusion The risk of bleeding, and therefore of transfusion, constitutes the major challenge of this
Anti-MHC Class II antibody conjugated to Biotin [ER-TR3] validated for IHC, Flow Cyt, ICC/IF and tested in Mouse. Referenced in 1 publication. Immunogen…
Summary In a previous study we showed that allografts of BN fetal bone, unlike allografts of adult bone, are not rejected by allogeneic recipients of the Lewis strain in spite of the existence of major histocompatibility complex (MHC) incompatibility between donors and hosts. In the present study,...
Islet transplantation has been reported to induce allosensitization in the majority of type 1 diabetic recipients of fresh or shortly incubated islet grafts prepared from one to three donors ...
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The clinical use of intravenous immunoglobulin (IVIg) has extended beyond its traditional place in the treating patients with primary immunodeficiencies. (HLA)-sensitized patients to Pitavastatin calcium inhibitor database lessen anti-HLA antibody amounts, thereby enabling transplantation in these sufferers. Second of all, IVIg in conjunction with Pitavastatin calcium inhibitor database rituximab works well in the treating antibody-mediated rejection pursuing transplantation. Treatment with polyclonal IVIg is normally a promising adjunctive therapy for serious sepsis and septic shock, but its make use of continues to be controversial and additional study is necessary before it could be suggested routinely. This review covers brand-new advancements in these areas and highlights the wide range of potential therapeutic areas where IVIg may possess a scientific impact. Pitavastatin calcium inhibitor database = 0035). Nevertheless, this was a brief trial of just three months, so theres still ...
In rare cases, transplants between a donor and recipient who have different blood types may occur by using medications to reduce the chance of a reaction. This is called ABO-incompatible transplantation.. • Human Leukocyte Antigens (HLA) and Panel Reactive Antibody (PRA): These tests help determine the likelihood of success of an organ transplant by checking for antibodies in your childs blood. Antibodies are made by the bodys immune system in reaction to a foreign substance, such as a blood transfusion, a virus, or a transplanted organ, and women may also develop antibodies during pregnancy. Antibodies in the bloodstream will try to attack transplanted organs, therefore, people who receive a transplant must take medications called immunosuppressants that decrease this immune response. • Viral Studies: These tests determine if your child has been exposed to viruses that may recur after transplant and help us to tailor your childs medication regimen after transplant. Diagnostic tests that ...
2017 (English)In: Transplant International, ISSN 0934-0874, E-ISSN 1432-2277, Vol. 30, p. 163-163Article in journal, Meeting abstract (Other academic) Published ...
Based on the structural similarity between B cell receptor and immunoglobulin binding sites, it is postulated that HLA-specific B cells should bind to HLA molecules with specificity comparable to that of the secreted immunoglobulin. Indeed, identification of HLA specifc B cells by staining through binding of the B cell receptor using 﫿uorescently labeled tetramers of identified HLA class I specifcities has been described [13]. A different approach is to utilize commercially available single antigen coated, color-coded microspheres, multiplexed in an assay that is currently the mainstay of soluble antibody detection in the circulation [14, 15, 16]. However, this powerful assay has also highlighted the challenges of cross- and poly-reactivity of allo-antibodies [17, 18] have recently described a method utilizing single HLA coated beads to enumerate HLA committed B cells [18]. Class I HSB identified in non-transplanted individuals were described by [19] who identified HLA specific B cells using ...
15-8-11 Parentage tests. (a) In a proceeding under this chapter before trial, the court, upon application made by or on behalf of any party to the action, and supported by sworn affidavit, or on its own motion, shall order that the mother, child, alleged father, and any other party to the action submit to blood or tissue typing tests which may include, but are not limited to, tests of red cell antigens, red cell isoenzymes, human leukocyte antigens, serum proteins, DNA and other genetic testing, to determine whether the alleged father is likely to be, or is not, the father of the child. The sworn affidavit must include a statement alleging paternity and setting forth facts establishing a reasonable possibility of sexual contact during the probable period of conception or a statement denying paternity and setting forth facts establishing a reasonable possibility of the nonexistence of sexual contact during the probable period of conception. In a proceeding to establish paternity and/or support ...
This years extra mural meeting was opened by Sebastiaan Heidt from the ETRL who discussed the pilot on patient-based external proficiency testing (EPT) cases. First, the paper-based EPT that was sent around in July 2015 was discussed. The results from this exercise showed a striking diversity in the advice on whether the transplant could go ahead or not. Some important issues came up, such as what to do in case a crossmatch without DTT is positive, the crossmatch with DTT is negative, and no information is available on autologous crossmatch results. Furthermore, there was lots of discussion on how to deal with HLA antibodies detected by Luminex only in case of a negative crossmatch. Some practical issues, such as how to deal with the prozone effect in Luminex assays were discussed. It was clear that center-specific policies on the value of antibodies detected by Luminex only are quite different. Next, the patient-based EPT on actual samples used for crossmatching was discussed. Also here, it ...
臺大位居世界頂尖大學之列,為永久珍藏及向國際展現本校豐碩的研究成果及學術能量,圖書館整合機構典藏(NTUR)與學術庫(AH)不同功能平台,成為臺大學術典藏NTU scholars。期能整合研究能量、促進交流合作、保存學術產出、推廣研究成果。. To permanently archive and promote researcher profiles and scholarly works, Library integrates the services of NTU Repository with Academic Hub to form NTU Scholars.. ...
We periodically host webinars where industry leaders can discuss HLA antibody detection and DNA typing related techniques and standards.
IN HUMAN BLOOD REACTING WITH ANTI-RHESUS SERA AND WITH HUMAN ISOANTIBODIES". Journal of Experimental Medicine. 74 (4): 309-320 ...
... in human blood reacting with anti-rhesus sera and with human isoantibodies". J Exp Med. 74 (4): 309-320. doi:10.1084/jem.74.4. ...
"Studies on an agglutinogen (Rh) in human blood reacting with anti-rhesus sera and with human isoantibodies". J Exp Med 74 (4 ...
Irregular antibodies are all non-ABO antibodies, although the main use of the term is for non-ABO isoantibodies that may cause ...
... isoantibodies MeSH D12.776.377.715.548.114.715 - oligoclonal bands MeSH D12.776.377.715.548.114.767 - opsonin proteins MeSH ...
... isoantibodies MeSH D12.776.124.486.485.114.715 - oligoclonal bands MeSH D12.776.124.486.485.114.767 - opsonin proteins MeSH ... isoantibodies MeSH D12.776.124.790.651.114.715 - oligoclonal bands MeSH D12.776.124.790.651.114.767 - opsonin proteins MeSH ...
... receptor Intracellular antibody-mediated degradation Intravascular immunity Intrinsic immunity IRGs ISCOM Isoantibodies ...
... are seen in people with different blood groups. The anti-A or anti-B isoantibodies or both (also called ... In a person with A blood group, the plasma will contain isoantibodies against B antigens, so immediately after transfusion of ... Isoantibodies, formerly called alloantibodies, are antibodies produced by an individual against isoantigens produced by members ... When antigens from another individual are introduced into another's body, these isoantibodies immediately bind to and destroy ...
இவற்றை ஓரின பிறபொருளெதிரிகள் (isoantibodies) எனலாம். அதாவது, அவை ஓரின பிறபொருளெதிரியாக்கிகளுக்கு எதிராக உருவாக்கப்படுபவையாகும் ... ஒவ்வொரு குருதி வகையிலும், தன்னுடல் சாராத குருதிப் பிறபொருளெதிரியாக்கிகளுக்கு எதிரான சம பிறபொருளெதிரிகள் (isoantibodies) ... isoantibodies) உருவாகலாம். எனவே பாம்பே தோற்றவமைப்பு உடையவர்களுக்கு O குருதிவகை வழங்கப்படுமாயின், அங்கே எதிர் - H ...
Isoantibodies are seen in people with different blood groups. The anti-A or anti-B isoantibodies or both (also called ... In a person with A blood group, the plasma will contain isoantibodies against B antigens, so immediately after transfusion of ... Isoantibodies, formerly called alloantibodies, are antibodies produced by an individual against isoantigens produced by members ... When antigens from another individual are introduced into anothers body, these isoantibodies immediately bind to and destroy ...
Make research projects and school reports about Isoantibodies easy with credible articles from our FREE, online encyclopedia ... Isoantibodies are present in human serum. An individual possesses isoantibodies to the opposite A or B isoantigen. For example ... Isoantibodies are present in human serum. An individual possesses isoantibodies to the opposite A or B isoantigen. For example ... Isoantibodies. Isoantibodies are antibodies (proteins that defend against foreign agents, such as bacteria) in blood . ...
"Isoantibodies" is a descriptor in the National Library of Medicines controlled vocabulary thesaurus, MeSH (Medical Subject ... This graph shows the total number of publications written about "Isoantibodies" by people in this website by year, and whether ... Below are the most recent publications written about "Isoantibodies" by people in Profiles. ...
CYTOTOXIC EFFECT OF ISOANTIBODIES ON MOUSE TUMOR CELLS IN VITRO. HELLSTRÖM, K. E. ...
Isoantibodies / metabolism* * Kidney Transplantation / immunology* * Postoperative Period * Prognosis * Tissue Donors* * ...
Possible role of isoantibodies to pancreatic antigens in diabetes. Possible role of isoantibodies to pancreatic antigens in ... Isoantibodies Language: English Journal: Indian J Exp Biol Year: 1982 Type: Article ... Isoantibodies Language: English Journal: Indian J Exp Biol Year: 1982 Type: Article ...
Isoantibodies / blood * Middle Aged * Opportunistic Infections / epidemiology * Portal Vein * Reproducibility of Results ...
0 (Biomarkers); 0 (Isoantibodies); 0 (Platelet Glycoprotein GPIIb-IIIa Complex); 0 (Platelet Glycoprotein GPIb-IX Complex); 0 ( ...
Isoantibodies / immunology. Liver Transplantation / immunology*. Male. Rats. Rats, Inbred ACI. Rats, Inbred Lew. Splenectomy*. ...
Isoantibodies / therapeutic use*. Male. Middle Aged. Opsonin Proteins / therapeutic use*. Platelet Count. Prospective Studies. ... 0/Immunosuppressive Agents; 0/Isoantibodies; 0/Opsonin Proteins; 0/RHO(D) antibody; 0/Receptors, IgG; 0/Rh-Hr Blood-Group ...
Auto- and Isoantibodies to Collagen in Diseases of Connective Tissue. IX. Pathogenicity of Anticollagen Antibodies. X. Summary ...
Isoantibodies · Lymphocyte Culture Test, Mixed · Macaca mulatta · Male ...
The cytotoxic activity of isoantibodies in mice. Transplant Bull 1956; 3: 142-143.. *Web of Science® Times Cited: 795 ...
Reactions of maternal isoantibodies responsible for neonatal purpura. Differentiation of a second platelet antigen system. J. ...
Isoantibodies (analysis) *Thrombocytopenia (diagnosis, immunology) Join CureHunter, for free Research Interface BASIC access!. ...
Isoantibodies (administration & dosage, adverse effects, therapeutic use) *Male. *Pharmacokinetics. *Platelet Count. *Purpura, ...
Normally occurring iso-antibodies in cattle blood. Onderstepoort Journal of Veterinary Research 29: 89-106 [ Links ]. 8. ...
These precipitating isoantibodies react with a protein in the blood of some normal and some ... Hemophilia patients and others who have received multiple transfusions may develop precipitating isoantibodies against ...
IN HUMAN BLOOD REACTING WITH ANTI-RHESUS SERA AND WITH HUMAN ISOANTIBODIES". Journal of Experimental Medicine. 74 (4): 309-320 ...
Svejgaard A, Kissmeyer-Nielsen F (1968) Cross-reactive human HL-A isoantibodies. Nature 219: 868-869.PubMedCrossRefGoogle ... Gorer PA, OGorman P (1956) The cytotoxic activity of isoantibodies in mice. Transplant Bull 3:142-143.Google Scholar ...
Biologic Properties of 19 S and 7 S Mouse Isoantibodies Directed against Isoantigens of the H-2 System Göran Möller ...
Dausset J. Colin M. et Colombani J. : Immune platelet iso-antibodies. Vox Sang. 5 : 4-31, 1960. Dausset J., Colombani J. et ... Reaction in thedetection of human iso-antibodies against leucocytes, platelets and Hela cells. Brit. J. Haemat. 5 : 225-231, ...
"Studies on an agglutinogen (Rh) in human blood reacting with anti-rhesus sera and with human isoantibodies". J Exp Med 74 (4 ...
Effect of ionic strength on the serologic behavior of red cell isoantibodies. ...
or n immunol the development of isoantibodies within an individual in order to protect against antigens derived from a ... Physiology) immunol the development of isoantibodies within an individual in order to protect against antigens derived from a ...
The reaction occurred because the patient had no A1 antigen to neutralize the A1 isoantibodies in the O blood. These antibodies ... Further Observations on In Vivo and In Vitro Behavior of Isoantibodies of Immune Type Present in Group O Blood. Blood 5: 553, ... The isoantibodies transfused into these patients were of all varieties: warm and cold agglutinins, hemolysins incomplete ... This was probably due to the activity of transfused isoantibodies in the plasma of the O blood. This hemolytic activity was ...
Red cell transfusion workflow in 71 patients having warm autoantibodies evaluated with the combination of dilution technique and red cell phenotyping.
Approximately 95% of type B queens have strong isoantibodies against type A blood. ...
  • When antigens from another individual are introduced into another's body, these isoantibodies immediately bind to and destroy them. (wikipedia.org)
  • The anti-A or anti-B isoantibodies or both (also called isohaemagglutinins) are produced by an individual against the antigens (A or B) on the RBCs of other blood groups. (wikipedia.org)
  • In a person with A blood group, the plasma will contain isoantibodies against B antigens, so immediately after transfusion of blood from B group the anti-B isohemagglutinins agglutinate the foreign red blood cells. (wikipedia.org)
  • Possible role of isoantibodies to pancreatic antigens in diabetes. (bvsalud.org)
  • Immune Hemolytic Anemia is an acquired hemolytic anemia in which a hemolytic response is caused by isoantibodies or autoantibodies produced on exposure to drugs, toxins, or other antigens. (diagnose-me.com)
  • The quantitative expression of ABH antigens on individual thyroid cells was variable and the cell-surface IFL pattern due to binding of blood group isoantibodies was different from that given by organ-specific thyroid autoantibodies on viable cultures. (rupress.org)
  • With RBCs in particular, you may see the antigens referred to as isoantigens or agglutinogens (surface antigens) and the antibodies referred to as isoantibodies or agglutinins. (lumenlearning.com)
  • Isoantibodies are antibodies (proteins that defend against foreign agents, such as bacteria) in blood. (encyclopedia.com)
  • Isoantibodies, formerly called alloantibodies, are antibodies produced by an individual against isoantigens produced by members of the same species. (wikipedia.org)
  • Isoantibodies to sperm cells are not significantly predominant, but autoimmunity is well expressed in IgG positivity against phosphatidyl inositol, phosphatidyl ethanolamine, phosphatidyl serine, cardiolipin, and beta-2 glycoprotein I, as well as antizona pellucida antibodies in IgG isotype. (hindawi.com)
  • R.A. Bronson, G.W. Cooper, D.L. Rosenfeld, Sperm specific isoantibodies and autoantibodies inhibit the binding of human sperm to the human zona pellucida, Fertil Steril 38: 724 (1984). (springer.com)
  • The paper notes the presence of autoantibodies to zona pellucida, to phospholipids (phosphatidyl serine, phosphatidyl ethanolamine, phosphatidyl inositol, phosphatidyl glycerol, phosphatidic acid, annexin V, beta-2 glycoprotein I, and cardiolipin) and of isoantibodies to sperm cells. (hindawi.com)
  • ABSTRACT Anti-zona-pellucida autoantibodies (AZP-Ab) and anti-sperm isoantibodies (ASA) were assessed in the cervical secretions from 73 infertile Jordanian women and 41 fertile control women using latex agglutination. (who.int)
  • Seventy-five percent of autoantibodies, but none of the isoantibodies, did not react with CD177/PR3 immobilized with moab MEM166. (bvsalud.org)
  • The majority of autoantibodies did not react with recombinant CD177, whereas most isoantibodies tested positive. (bvsalud.org)
  • Isoantibodies mainly target CD177 alone, while the majority of autoantibodies target a native epitope present on the neutrophil surface, but absent from recombinant CD177 which lacks PR3. (bvsalud.org)
  • Isoantibodies are present in human serum. (encyclopedia.com)
  • For example, persons of blood type A will have serum containing isoantibodies to the B antigen. (encyclopedia.com)
  • Isoantibodies" is a descriptor in the National Library of Medicine's controlled vocabulary thesaurus, MeSH (Medical Subject Headings) . (umassmed.edu)
  • Furthermore, the possibility that blood group natural isoantibodies bind to the cell surface must be taken into account in experiments in which cultured thyroid cells are exposed to human sera. (rupress.org)
  • In 1929, Kosaku Yosida established the existence of isoantibodies in body fluids other than blood (saliva, sinus secretions,etc. (encyclopedia.com)
  • By establishing the existence of serological isoantibodies in fluids other than blood, Yosida paved the way for far more sophistication in the forensic analysis of bodily fluids, along with the ability to use multiple means of identification of a single perpetrator of a crime. (encyclopedia.com)
  • Isoantibodies are seen in people with different blood groups. (wikipedia.org)
  • If, for example, 20 pints of O blood were given to a subject of Group A, B, or AB, would the accumulation of isoantibodies finally become pathogenic and destroy the recipient's native red cells? (army.mil)
  • Approximately 95% of type B queens have strong isoantibodies against type A blood. (vin.com)
  • An individual possesses isoantibodies to the opposite A or B isoantigen. (encyclopedia.com)
  • This graph shows the total number of publications written about "Isoantibodies" by people in this website by year, and whether "Isoantibodies" was a major or minor topic of these publications. (umassmed.edu)
  • Below are the most recent publications written about "Isoantibodies" by people in Profiles. (umassmed.edu)