A soluble factor produced by MONOCYTES; MACROPHAGES, and other cells which activates T-lymphocytes and potentiates their response to mitogens or antigens. Interleukin-1 is a general term refers to either of the two distinct proteins, INTERLEUKIN-1ALPHA and INTERLEUKIN-1BETA. The biological effects of IL-1 include the ability to replace macrophage requirements for T-cell activation.
A soluble substance elaborated by antigen- or mitogen-stimulated T-LYMPHOCYTES which induces DNA synthesis in naive lymphocytes.
Cell surface proteins that bind interleukins and trigger intracellular changes influencing the behavior of cells.
A cytokine that stimulates the growth and differentiation of B-LYMPHOCYTES and is also a growth factor for HYBRIDOMAS and plasmacytomas. It is produced by many different cells including T-LYMPHOCYTES; MONOCYTES; and FIBROBLASTS.
Non-antibody proteins secreted by inflammatory leukocytes and some non-leukocytic cells, that act as intercellular mediators. They differ from classical hormones in that they are produced by a number of tissue or cell types rather than by specialized glands. They generally act locally in a paracrine or autocrine rather than endocrine manner.
A soluble factor produced by activated T-LYMPHOCYTES that induces the expression of MHC CLASS II GENES and FC RECEPTORS on B-LYMPHOCYTES and causes their proliferation and differentiation. It also acts on T-lymphocytes, MAST CELLS, and several other hematopoietic lineage cells.
A cytokine produced by a variety of cell types, including T-LYMPHOCYTES; MONOCYTES; DENDRITIC CELLS; and EPITHELIAL CELLS that exerts a variety of effects on immunoregulation and INFLAMMATION. Interleukin-10 combines with itself to form a homodimeric molecule that is the biologically active form of the protein.
Soluble factors which stimulate growth-related activities of leukocytes as well as other cell types. They enhance cell proliferation and differentiation, DNA synthesis, secretion of other biologically active molecules and responses to immune and inflammatory stimuli.
A member of the CXC chemokine family that plays a role in the regulation of the acute inflammatory response. It is secreted by variety of cell types and induces CHEMOTAXIS of NEUTROPHILS and other inflammatory cells.
A ligand that binds to but fails to activate the INTERLEUKIN 1 RECEPTOR. It plays an inhibitory role in the regulation of INFLAMMATION and FEVER. Several isoforms of the protein exist due to multiple ALTERNATIVE SPLICING of its mRNA.
Serum glycoprotein produced by activated MACROPHAGES and other mammalian MONONUCLEAR LEUKOCYTES. It has necrotizing activity against tumor cell lines and increases ability to reject tumor transplants. Also known as TNF-alpha, it is only 30% homologous to TNF-beta (LYMPHOTOXIN), but they share TNF RECEPTORS.
Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others.
Receptors present on activated T-LYMPHOCYTES and B-LYMPHOCYTES that are specific for INTERLEUKIN-2 and play an important role in LYMPHOCYTE ACTIVATION. They are heterotrimeric proteins consisting of the INTERLEUKIN-2 RECEPTOR ALPHA SUBUNIT, the INTERLEUKIN-2 RECEPTOR BETA SUBUNIT, and the INTERLEUKIN RECEPTOR COMMON GAMMA-CHAIN.
The major interferon produced by mitogenically or antigenically stimulated LYMPHOCYTES. It is structurally different from TYPE I INTERFERON and its major activity is immunoregulation. It has been implicated in the expression of CLASS II HISTOCOMPATIBILITY ANTIGENS in cells that do not normally produce them, leading to AUTOIMMUNE DISEASES.
Lymphocytes responsible for cell-mediated immunity. Two types have been identified - cytotoxic (T-LYMPHOCYTES, CYTOTOXIC) and helper T-lymphocytes (T-LYMPHOCYTES, HELPER-INDUCER). They are formed when lymphocytes circulate through the THYMUS GLAND and differentiate to thymocytes. When exposed to an antigen, they divide rapidly and produce large numbers of new T cells sensitized to that antigen.
A heterodimeric cytokine that plays a role in innate and adaptive immune responses. Interleukin-12 is a 70 kDa protein that is composed of covalently linked 40 kDa and 35 kDa subunits. It is produced by DENDRITIC CELLS; MACROPHAGES and a variety of other immune cells and plays a role in the stimulation of INTERFERON-GAMMA production by T-LYMPHOCYTES and NATURAL KILLER CELLS.
Morphologic alteration of small B LYMPHOCYTES or T LYMPHOCYTES in culture into large blast-like cells able to synthesize DNA and RNA and to divide mitotically. It is induced by INTERLEUKINS; MITOGENS such as PHYTOHEMAGGLUTININS, and by specific ANTIGENS. It may also occur in vivo as in GRAFT REJECTION.
RNA sequences that serve as templates for protein synthesis. Bacterial mRNAs are generally primary transcripts in that they do not require post-transcriptional processing. Eukaryotic mRNA is synthesized in the nucleus and must be exported to the cytoplasm for translation. Most eukaryotic mRNAs have a sequence of polyadenylic acid at the 3' end, referred to as the poly(A) tail. The function of this tail is not known for certain, but it may play a role in the export of mature mRNA from the nucleus as well as in helping stabilize some mRNA molecules by retarding their degradation in the cytoplasm.
Proteins prepared by recombinant DNA technology.
Cell surface receptors that are specific for INTERLEUKIN-1. Included under this heading are signaling receptors, non-signaling receptors and accessory proteins required for receptor signaling. Signaling from interleukin-1 receptors occurs via interaction with SIGNAL TRANSDUCING ADAPTOR PROTEINS such as MYELOID DIFFERENTIATION FACTOR 88.
An interleukin-1 subtype that is synthesized as an inactive membrane-bound pro-protein. Proteolytic processing of the precursor form by CASPASE 1 results in release of the active form of interleukin-1beta from the membrane.
A multilineage cell growth factor secreted by LYMPHOCYTES; EPITHELIAL CELLS; and ASTROCYTES which stimulates clonal proliferation and differentiation of various types of blood and tissue cells.
A cytokine that promotes differentiation and activation of EOSINOPHILS. It also triggers activated B-LYMPHOCYTES to differentiate into IMMUNOGLOBULIN-secreting cells.
A cytokine which resembles IL-1 structurally and IL-12 functionally. It enhances the cytotoxic activity of NK CELLS and CYTOTOXIC T-LYMPHOCYTES, and appears to play a role both as neuroimmunomodulator and in the induction of mucosal immunity.
Lipid-containing polysaccharides which are endotoxins and important group-specific antigens. They are often derived from the cell wall of gram-negative bacteria and induce immunoglobulin secretion. The lipopolysaccharide molecule consists of three parts: LIPID A, core polysaccharide, and O-specific chains (O ANTIGENS). When derived from Escherichia coli, lipopolysaccharides serve as polyclonal B-cell mitogens commonly used in laboratory immunology. (From Dorland, 28th ed)
Receptors present on a wide variety of hematopoietic and non-hematopoietic cell types that are specific for INTERLEUKIN-4. They are involved in signaling a variety of immunological responses related to allergic INFLAMMATION including the differentiation of TH2 CELLS and the regulation of IMMUNOGLOBULIN E production. Two subtypes of receptors exist and are referred to as the TYPE I INTERLEUKIN-4 RECEPTOR and the TYPE II INTERLEUKIN-4 RECEPTOR. Each receptor subtype is defined by its unique subunit composition.
A pathological process characterized by injury or destruction of tissues caused by a variety of cytologic and chemical reactions. It is usually manifested by typical signs of pain, heat, redness, swelling, and loss of function.
A cytokine synthesized by T-LYMPHOCYTES that produces proliferation, immunoglobulin isotype switching, and immunoglobulin production by immature B-LYMPHOCYTES. It appears to play a role in regulating inflammatory and immune responses.
Established cell cultures that have the potential to propagate indefinitely.
Cell surface receptors that are specific for INTERLEUKIN-6. They are present on T-LYMPHOCYTES, mitogen-activated B-LYMPHOCYTES, and peripheral MONOCYTES. The receptors are heterodimers of the INTERLEUKIN-6 RECEPTOR ALPHA SUBUNIT and the CYTOKINE RECEPTOR GP130.
A lymphohematopoietic cytokine that plays a role in regulating the proliferation of ERYTHROID PRECURSOR CELLS. It induces maturation of MEGAKARYOCYTES which results in increased production of BLOOD PLATELETS. Interleukin-11 was also initially described as an inhibitor of ADIPOGENESIS of cultured preadipocytes.
Large, phagocytic mononuclear leukocytes produced in the vertebrate BONE MARROW and released into the BLOOD; contain a large, oval or somewhat indented nucleus surrounded by voluminous cytoplasm and numerous organelles.
The fission of a CELL. It includes CYTOKINESIS, when the CYTOPLASM of a cell is divided, and CELL NUCLEUS DIVISION.
Any of the processes by which nuclear, cytoplasmic, or intercellular factors influence the differential control (induction or repression) of gene action at the level of transcription or translation.
The intracellular transfer of information (biological activation/inhibition) through a signal pathway. In each signal transduction system, an activation/inhibition signal from a biologically active molecule (hormone, neurotransmitter) is mediated via the coupling of a receptor/enzyme to a second messenger system or to an ion channel. Signal transduction plays an important role in activating cellular functions, cell differentiation, and cell proliferation. Examples of signal transduction systems are the GAMMA-AMINOBUTYRIC ACID-postsynaptic receptor-calcium ion channel system, the receptor-mediated T-cell activation pathway, and the receptor-mediated activation of phospholipases. Those coupled to membrane depolarization or intracellular release of calcium include the receptor-mediated activation of cytotoxic functions in granulocytes and the synaptic potentiation of protein kinase activation. Some signal transduction pathways may be part of larger signal transduction pathways; for example, protein kinase activation is part of the platelet activation signal pathway.
Cell surface molecules on cells of the immune system that specifically bind surface molecules or messenger molecules and trigger changes in the behavior of cells. Although these receptors were first identified in the immune system, many have important functions elsewhere.
Subset of helper-inducer T-lymphocytes which synthesize and secrete the interleukins IL-4, IL-5, IL-6, and IL-10. These cytokines influence B-cell development and antibody production as well as augmenting humoral responses.
An immunoassay utilizing an antibody labeled with an enzyme marker such as horseradish peroxidase. While either the enzyme or the antibody is bound to an immunosorbent substrate, they both retain their biologic activity; the change in enzyme activity as a result of the enzyme-antibody-antigen reaction is proportional to the concentration of the antigen and can be measured spectrophotometrically or with the naked eye. Many variations of the method have been developed.
A proinflammatory cytokine produced primarily by T-LYMPHOCYTES or their precursors. Several subtypes of interleukin-17 have been identified, each of which is a product of a unique gene.
Technique using an instrument system for making, processing, and displaying one or more measurements on individual cells obtained from a cell suspension. Cells are usually stained with one or more fluorescent dyes specific to cell components of interest, e.g., DNA, and fluorescence of each cell is measured as it rapidly transverses the excitation beam (laser or mercury arc lamp). Fluorescence provides a quantitative measure of various biochemical and biophysical properties of the cell, as well as a basis for cell sorting. Other measurable optical parameters include light absorption and light scattering, the latter being applicable to the measurement of cell size, shape, density, granularity, and stain uptake.
Differentiation antigens residing on mammalian leukocytes. CD stands for cluster of differentiation, which refers to groups of monoclonal antibodies that show similar reactivity with certain subpopulations of antigens of a particular lineage or differentiation stage. The subpopulations of antigens are also known by the same CD designation.
The relatively long-lived phagocytic cell of mammalian tissues that are derived from blood MONOCYTES. Main types are PERITONEAL MACROPHAGES; ALVEOLAR MACROPHAGES; HISTIOCYTES; KUPFFER CELLS of the liver; and OSTEOCLASTS. They may further differentiate within chronic inflammatory lesions to EPITHELIOID CELLS or may fuse to form FOREIGN BODY GIANT CELLS or LANGHANS GIANT CELLS. (from The Dictionary of Cell Biology, Lackie and Dow, 3rd ed.)
An encapsulated lymphatic organ through which venous blood filters.
Cytokine that stimulates the proliferation of T-LYMPHOCYTES and shares biological activities with IL-2. IL-15 also can induce proliferation and differentiation of B-LYMPHOCYTES.
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
A cytokine produced by bone marrow stromal cells that promotes the growth of B-LYMPHOCYTE precursors and is co-mitogenic with INTERLEUKIN-2 for mature T-LYMPHOCYTE activation.
Mature LYMPHOCYTES and MONOCYTES transported by the blood to the body's extravascular space. They are morphologically distinguishable from mature granulocytic leukocytes by their large, non-lobed nuclei and lack of coarse, heavily stained cytoplasmic granules.
A critical subpopulation of T-lymphocytes involved in the induction of most immunological functions. The HIV virus has selective tropism for the T4 cell which expresses the CD4 phenotypic marker, a receptor for HIV. In fact, the key element in the profound immunosuppression seen in HIV infection is the depletion of this subset of T-lymphocytes.
Cells grown in vitro from neoplastic tissue. If they can be established as a TUMOR CELL LINE, they can be propagated in cell culture indefinitely.
The phenotypic manifestation of a gene or genes by the processes of GENETIC TRANSCRIPTION and GENETIC TRANSLATION.
Subset of helper-inducer T-lymphocytes which synthesize and secrete interleukin-2, gamma-interferon, and interleukin-12. Due to their ability to kill antigen-presenting cells and their lymphokine-mediated effector activity, Th1 cells are associated with vigorous delayed-type hypersensitivity reactions.
Bone marrow-derived lymphocytes that possess cytotoxic properties, classically directed against transformed and virus-infected cells. Unlike T CELLS; and B CELLS; NK CELLS are not antigen specific. The cytotoxicity of natural killer cells is determined by the collective signaling of an array of inhibitory and stimulatory CELL SURFACE RECEPTORS. A subset of T-LYMPHOCYTES referred to as NATURAL KILLER T CELLS shares some of the properties of this cell type.
Antibodies produced by a single clone of cells.
Ubiquitous, inducible, nuclear transcriptional activator that binds to enhancer elements in many different cell types and is activated by pathogenic stimuli. The NF-kappa B complex is a heterodimer composed of two DNA-binding subunits: NF-kappa B1 and relA.
An interleukin receptor subunit with specificity for INTERLEUKIN-13. It dimerizes with the INTERLEUKIN-4 RECEPTOR ALPHA SUBUNIT to form the TYPE II INTERLEUKIN-4 RECEPTOR which has specificity for both INTERLEUKIN-4 and INTERLEUKIN-13. Signaling of this receptor subunit occurs through the interaction of its cytoplasmic domain with JANUS KINASES such as the TYK2 KINASE.
The endogenous compounds that mediate inflammation (AUTACOIDS) and related exogenous compounds including the synthetic prostaglandins (PROSTAGLANDINS, SYNTHETIC).
The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.
Cell surface receptors for INTERLEUKIN-13. Included under this heading are the INTERLEUKIN-13 RECEPTOR ALPHA2 which is a monomeric receptor and the INTERLEUKIN-4 RECEPTOR TYPE II which has specificity for both INTERLEUKIN-4 and INTERLEUKIN-13.
Lymphoid cells concerned with humoral immunity. They are short-lived cells resembling bursa-derived lymphocytes of birds in their production of immunoglobulin upon appropriate stimulation.
A variation of the PCR technique in which cDNA is made from RNA via reverse transcription. The resultant cDNA is then amplified using standard PCR protocols.
Soluble protein factors generated by activated lymphocytes that affect other cells, primarily those involved in cellular immunity.
Strains of mice in which certain GENES of their GENOMES have been disrupted, or "knocked-out". To produce knockouts, using RECOMBINANT DNA technology, the normal DNA sequence of the gene being studied is altered to prevent synthesis of a normal gene product. Cloned cells in which this DNA alteration is successful are then injected into mouse EMBRYOS to produce chimeric mice. The chimeric mice are then bred to yield a strain in which all the cells of the mouse contain the disrupted gene. Knockout mice are used as EXPERIMENTAL ANIMAL MODELS for diseases (DISEASE MODELS, ANIMAL) and to clarify the functions of the genes.
Subpopulation of CD4+ lymphocytes that cooperate with other lymphocytes (either T or B) to initiate a variety of immune functions. For example, helper-inducer T-cells cooperate with B-cells to produce antibodies to thymus-dependent antigens and with other subpopulations of T-cells to initiate a variety of cell-mediated immune functions.
The phenomenon of target cell destruction by immunologically active effector cells. It may be brought about directly by sensitized T-lymphocytes or by lymphoid or myeloid "killer" cells, or it may be mediated by cytotoxic antibody, cytotoxic factor released by lymphoid cells, or complement.
Progressive restriction of the developmental potential and increasing specialization of function that leads to the formation of specialized cells, tissues, and organs.
Glycoproteins which contain sialic acid as one of their carbohydrates. They are often found on or in the cell or tissue membranes and participate in a variety of biological activities.
The relationship between the dose of an administered drug and the response of the organism to the drug.
A signal transducer and activator of transcription that mediates cellular responses to INTERLEUKIN-6 family members. STAT3 is constitutively activated in a variety of TUMORS and is a major downstream transducer for the CYTOKINE RECEPTOR GP130.
White blood cells formed in the body's lymphoid tissue. The nucleus is round or ovoid with coarse, irregularly clumped chromatin while the cytoplasm is typically pale blue with azurophilic (if any) granules. Most lymphocytes can be classified as either T or B (with subpopulations of each), or NATURAL KILLER CELLS.
Antigens expressed on the cell membrane of T-lymphocytes during differentiation, activation, and normal and neoplastic transformation. Their phenotypic characterization is important in differential diagnosis and studies of thymic ontogeny and T-cell function.
Cytolytic lymphocytes with the unique capacity of killing natural killer (NK)-resistant fresh tumor cells. They are INTERLEUKIN-2-activated NK cells that have no MAJOR HISTOCOMPATIBILITY COMPLEX restriction or need for antigen stimulation. LAK cells are used for ADOPTIVE IMMUNOTHERAPY in cancer patients.
Signal molecules that are involved in the control of cell growth and differentiation.
Specialized cells of the hematopoietic system that have branch-like extensions. They are found throughout the lymphatic system, and in non-lymphoid tissues such as SKIN and the epithelia of the intestinal, respiratory, and reproductive tracts. They trap and process ANTIGENS, and present them to T-CELLS, thereby stimulating CELL-MEDIATED IMMUNITY. They are different from the non-hematopoietic FOLLICULAR DENDRITIC CELLS, which have a similar morphology and immune system function, but with respect to humoral immunity (ANTIBODY PRODUCTION).
Elements of limited time intervals, contributing to particular results or situations.
Naturally occurring or experimentally induced animal diseases with pathological processes sufficiently similar to those of human diseases. They are used as study models for human diseases.
The uptake of naked or purified DNA by CELLS, usually meaning the process as it occurs in eukaryotic cells. It is analogous to bacterial transformation (TRANSFORMATION, BACTERIAL) and both are routinely employed in GENE TRANSFER TECHNIQUES.
A positive regulatory effect on physiological processes at the molecular, cellular, or systemic level. At the molecular level, the major regulatory sites include membrane receptors, genes (GENE EXPRESSION REGULATION), mRNAs (RNA, MESSENGER), and proteins.
An interleukin-1 subtype that occurs as a membrane-bound pro-protein form that is cleaved by proteases to form a secreted mature form. Unlike INTERLEUKIN-1BETA both membrane-bound and secreted forms of interleukin-1alpha are biologically active.
Complex of at least five membrane-bound polypeptides in mature T-lymphocytes that are non-covalently associated with one another and with the T-cell receptor (RECEPTORS, ANTIGEN, T-CELL). The CD3 complex includes the gamma, delta, epsilon, zeta, and eta chains (subunits). When antigen binds to the T-cell receptor, the CD3 complex transduces the activating signals to the cytoplasm of the T-cell. The CD3 gamma and delta chains (subunits) are separate from and not related to the gamma/delta chains of the T-cell receptor (RECEPTORS, ANTIGEN, T-CELL, GAMMA-DELTA).
The rate dynamics in chemical or physical systems.
Mucoproteins isolated from the kidney bean (Phaseolus vulgaris); some of them are mitogenic to lymphocytes, others agglutinate all or certain types of erythrocytes or lymphocytes. They are used mainly in the study of immune mechanisms and in cell culture.
A heterodimeric cytokine that plays a role in innate and adaptive immune responses. Interleukin-23 is comprised of a unique 19 kDa subunit and 40 kDa subunit that is shared with INTERLEUKIN-12. It is produced by DENDRITIC CELLS; MACROPHAGES and a variety of other immune cells
An interleukin-1 receptor subtype that is involved in signaling cellular responses to INTERLEUKIN-1ALPHA and INTERLEUKIN-1BETA. The binding of this receptor to its ligand causes its favorable interaction with INTERLEUKIN-1 RECEPTOR ACCESSORY PROTEIN and the formation of an activated receptor complex.
Measurable and quantifiable biological parameters (e.g., specific enzyme concentration, specific hormone concentration, specific gene phenotype distribution in a population, presence of biological substances) which serve as indices for health- and physiology-related assessments, such as disease risk, psychiatric disorders, environmental exposure and its effects, disease diagnosis, metabolic processes, substance abuse, pregnancy, cell line development, epidemiologic studies, etc.
Genetically identical individuals developed from brother and sister matings which have been carried out for twenty or more generations, or by parent x offspring matings carried out with certain restrictions. All animals within an inbred strain trace back to a common ancestor in the twentieth generation.
A cytokine receptor that acts through the formation of oligomeric complexes of itself with a variety of CYTOKINE RECEPTORS.
Cell surface receptors that are specific for INTERLEUKIN-5. They are heterodimeric proteins consisting of the INTERLEUKIN-5 RECEPTOR ALPHA SUBUNIT and the CYTOKINE RECEPTOR COMMON BETA SUBUNIT. Signaling from interleukin-5 receptors can occur through interaction of their cytoplasmic domains with SYNTENINS.
A long pro-domain caspase that has specificity for the precursor form of INTERLEUKIN-1BETA. It plays a role in INFLAMMATION by catalytically converting the inactive forms of CYTOKINES such as interleukin-1beta to their active, secreted form. Caspase 1 is referred as interleukin-1beta converting enzyme and is frequently abbreviated ICE.
The biosynthesis of RNA carried out on a template of DNA. The biosynthesis of DNA from an RNA template is called REVERSE TRANSCRIPTION.
A signal transducer and activator of transcription that mediates cellular responses to INTERLEUKIN-4. Stat6 has been shown to partner with NF-KAPPA B and CCAAT-ENHANCER-BINDING PROTEINS to regulate GENETIC TRANSCRIPTION of interleukin-4 responsive GENES.
Glycoproteins found on the membrane or surface of cells.
The most common and most biologically active of the mammalian prostaglandins. It exhibits most biological activities characteristic of prostaglandins and has been used extensively as an oxytocic agent. The compound also displays a protective effect on the intestinal mucosa.
An immunoglobulin associated with MAST CELLS. Overexpression has been associated with allergic hypersensitivity (HYPERSENSITIVITY, IMMEDIATE).
Cell surface receptors for INTERLEUKIN-10. They exist as a tetramer of two alpha chains (INTERLEUKIN-10 RECEPTOR ALPHA CHAIN) and two beta chains (INTERLEUKIN-10 RECEPTOR, BETA CHAIN). Signaling from interleukin-10 receptors occurs through their interaction with JANUS KINASES.
Manipulation of the host's immune system in treatment of disease. It includes both active and passive immunization as well as immunosuppressive therapy to prevent graft rejection.
Cell surface receptors that are specific for INTERLEUKIN-7. They are present on T-LYMPHOCYTES and B-LYMPHOCYTE precursors. The receptors are heterodimeric proteins consisting of the INTERLEUKIN-5 RECEPTOR ALPHA SUBUNIT and the CYTOKINE RECEPTOR COMMON BETA SUBUNIT.
High affinity receptors for INTERLEUKIN-3. They are found on early HEMATOPOIETIC PROGENITOR CELLS; progenitors of MYELOID CELLS; EOSINOPHILS; and BASOPHILS. Interleukin-3 receptors are formed by the dimerization of the INTERLEUKIN-3 RECEPTOR ALPHA SUBUNIT and the CYTOKINE RECEPTOR COMMON BETA SUBUNIT.
A cytokine subunit that is a component of both interleukin-12 and interleukin-23. It binds to the INTERLEUKIN-12 SUBUNIT P35 via a disulfide bond to form interleukin-12 and to INTERLEUKIN-23 SUBUNIT P19 to form interleukin-23.
Molecules on the surface of T-lymphocytes that recognize and combine with antigens. The receptors are non-covalently associated with a complex of several polypeptides collectively called CD3 antigens (ANTIGENS, CD3). Recognition of foreign antigen and the major histocompatibility complex is accomplished by a single heterodimeric antigen-receptor structure, composed of either alpha-beta (RECEPTORS, ANTIGEN, T-CELL, ALPHA-BETA) or gamma-delta (RECEPTORS, ANTIGEN, T-CELL, GAMMA-DELTA) chains.
Either of the pair of organs occupying the cavity of the thorax that effect the aeration of the blood.
A classification of T-lymphocytes, especially into helper/inducer, suppressor/effector, and cytotoxic subsets, based on structurally or functionally different populations of cells.
Cells contained in the bone marrow including fat cells (see ADIPOCYTES); STROMAL CELLS; MEGAKARYOCYTES; and the immediate precursors of most blood cells.
Granular leukocytes with a nucleus that usually has two lobes connected by a slender thread of chromatin, and cytoplasm containing coarse, round granules that are uniform in size and stainable by eosin.
The action of a drug in promoting or enhancing the effectiveness of another drug.
A subunit of the interleukin-18 receptor that is responsible of extracellular binding of IL-18.
Progenitor cells from which all blood cells derive.
Granular leukocytes having a nucleus with three to five lobes connected by slender threads of chromatin, and cytoplasm containing fine inconspicuous granules and stainable by neutral dyes.
High-affinity G-protein-coupled receptors for INTERLEUKIN-8 present on NEUTROPHILS; MONOCYTES; and BASOPHILS.
A critical subpopulation of regulatory T-lymphocytes involved in MHC Class I-restricted interactions. They include both cytotoxic T-lymphocytes (T-LYMPHOCYTES, CYTOTOXIC) and CD8+ suppressor T-lymphocytes.
In vitro method for producing large amounts of specific DNA or RNA fragments of defined length and sequence from small amounts of short oligonucleotide flanking sequences (primers). The essential steps include thermal denaturation of the double-stranded target molecules, annealing of the primers to their complementary sequences, and extension of the annealed primers by enzymatic synthesis with DNA polymerase. The reaction is efficient, specific, and extremely sensitive. Uses for the reaction include disease diagnosis, detection of difficult-to-isolate pathogens, mutation analysis, genetic testing, DNA sequencing, and analyzing evolutionary relationships.
Antigens on surfaces of cells, including infectious or foreign cells or viruses. They are usually protein-containing groups on cell membranes or walls and may be isolated.
Cell surface receptors for INTERLEUKIN-18 found on a variety of cell types including MACROPHAGES; NEUTROPHILS; NK CELLS; ENDOTHELIAL CELLS; and SMOOTH MUSCLE CELLS. They are formed as a heterodimer of alpha and beta subunits.
DNA sequences which are recognized (directly or indirectly) and bound by a DNA-dependent RNA polymerase during the initiation of transcription. Highly conserved sequences within the promoter include the Pribnow box in bacteria and the TATA BOX in eukaryotes.
Class of pro-inflammatory cytokines that have the ability to attract and activate leukocytes. They can be divided into at least three structural branches: C; (CHEMOKINES, C); CC; (CHEMOKINES, CC); and CXC; (CHEMOKINES, CXC); according to variations in a shared cysteine motif.
One of the mechanisms by which CELL DEATH occurs (compare with NECROSIS and AUTOPHAGOCYTOSIS). Apoptosis is the mechanism responsible for the physiological deletion of cells and appears to be intrinsically programmed. It is characterized by distinctive morphologic changes in the nucleus and cytoplasm, chromatin cleavage at regularly spaced sites, and the endonucleolytic cleavage of genomic DNA; (DNA FRAGMENTATION); at internucleosomal sites. This mode of cell death serves as a balance to mitosis in regulating the size of animal tissues and in mediating pathologic processes associated with tumor growth.
The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.
Connective tissue cells which secrete an extracellular matrix rich in collagen and other macromolecules.
The introduction of a phosphoryl group into a compound through the formation of an ester bond between the compound and a phosphorus moiety.
Substances that reduce or suppress INFLAMMATION.
A phorbol ester found in CROTON OIL with very effective tumor promoting activity. It stimulates the synthesis of both DNA and RNA.
Laboratory mice that have been produced from a genetically manipulated EGG or EMBRYO, MAMMALIAN.
A MANNOSE/GLUCOSE binding lectin isolated from the jack bean (Canavalia ensiformis). It is a potent mitogen used to stimulate cell proliferation in lymphocytes, primarily T-lymphocyte, cultures.
Manifestations of the immune response which are mediated by antigen-sensitized T-lymphocytes via lymphokines or direct cytotoxicity. This takes place in the absence of circulating antibody or where antibody plays a subordinate role.
A chemokine that is a chemoattractant for MONOCYTES and may also cause cellular activation of specific functions related to host defense. It is produced by LEUKOCYTES of both monocyte and lymphocyte lineage and by FIBROBLASTS during tissue injury. It has specificity for CCR2 RECEPTORS.
Washing liquid obtained from irrigation of the lung, including the BRONCHI and the PULMONARY ALVEOLI. It is generally used to assess biochemical, inflammatory, or infection status of the lung.
Proteins which bind to DNA. The family includes proteins which bind to both double- and single-stranded DNA and also includes specific DNA binding proteins in serum which can be used as markers for malignant diseases.
A multifunctional cytokine secreted by primarily by activated TH2 CELLS that may play a role as a regulator of allergic INFLAMMATION. It has been shown to enhance the growth and CELL DIFFERENTIATION of MAST CELLS, and can act on a variety of other immune cells.
Cell surface receptors for INTERLEUKIN-12. They exist as dimers of beta 1 and beta 2 subunits. Signaling from interleukin-12 receptors occurs through their interaction with JANUS KINASES.
Soluble mediators of the immune response that are neither antibodies nor complement. They are produced largely, but not exclusively, by monocytes and macrophages.
Histochemical localization of immunoreactive substances using labeled antibodies as reagents.
Short sequences (generally about 10 base pairs) of DNA that are complementary to sequences of messenger RNA and allow reverse transcriptases to start copying the adjacent sequences of mRNA. Primers are used extensively in genetic and molecular biology techniques.
Subset of helper-effector T-lymphocytes which synthesize and secrete IL-17, IL-17F, and IL-22. These cytokines are involved in host defenses and tissue inflammation in autoimmune diseases.
A negative regulatory effect on physiological processes at the molecular, cellular, or systemic level. At the molecular level, the major regulatory sites include membrane receptors, genes (GENE EXPRESSION REGULATION), mRNAs (RNA, MESSENGER), and proteins.
Glycoproteins found in a subfraction of normal mammalian plasma and urine. They stimulate the proliferation of bone marrow cells in agar cultures and the formation of colonies of granulocytes and/or macrophages. The factors include INTERLEUKIN-3; (IL-3); GRANULOCYTE COLONY-STIMULATING FACTOR; (G-CSF); MACROPHAGE COLONY-STIMULATING FACTOR; (M-CSF); and GRANULOCYTE-MACROPHAGE COLONY-STIMULATING FACTOR; (GM-CSF).
Cell surface receptors for INTERLEUKIN-15. They are widely-distributed heterotrimeric proteins consisting of the INTERLEUKIN-15 RECEPTOR ALPHA SUBUNIT, the INTERLEUKIN-2, 15 RECEPTOR BETA SUBUNIT, and the INTERLEUKIN RECEPTOR COMMON GAMMA-CHAIN.
The capacity of a normal organism to remain unaffected by microorganisms and their toxins. It results from the presence of naturally occurring ANTI-INFECTIVE AGENTS, constitutional factors such as BODY TEMPERATURE and immediate acting immune cells such as NATURAL KILLER CELLS.
The process of altering the morphology and functional activity of macrophages so that they become avidly phagocytic. It is initiated by lymphokines, such as the macrophage activation factor (MAF) and the macrophage migration-inhibitory factor (MMIF), immune complexes, C3b, and various peptides, polysaccharides, and immunologic adjuvants.
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
Immunoglobulin molecules having a specific amino acid sequence by virtue of which they interact only with the ANTIGEN (or a very similar shape) that induced their synthesis in cells of the lymphoid series (especially PLASMA CELLS).
A plasma protein that circulates in increased amounts during inflammation and after tissue damage.
Cell surface receptors for INTERLEUKIN-17. Several subtypes of receptors have been found, each with its own in specificity for interleukin-17 subtype.
A malignant neoplasm derived from cells that are capable of forming melanin, which may occur in the skin of any part of the body, in the eye, or, rarely, in the mucous membranes of the genitalia, anus, oral cavity, or other sites. It occurs mostly in adults and may originate de novo or from a pigmented nevus or malignant lentigo. Melanomas frequently metastasize widely, and the regional lymph nodes, liver, lungs, and brain are likely to be involved. The incidence of malignant skin melanomas is rising rapidly in all parts of the world. (Stedman, 25th ed; from Rook et al., Textbook of Dermatology, 4th ed, p2445)
Inflammation of the COLON section of the large intestine (INTESTINE, LARGE), usually with symptoms such as DIARRHEA (often with blood and mucus), ABDOMINAL PAIN, and FEVER.
The inner membrane of a joint capsule surrounding a freely movable joint. It is loosely attached to the external fibrous capsule and secretes SYNOVIAL FLUID.
The span of viability of a cell characterized by the capacity to perform certain functions such as metabolism, growth, reproduction, some form of responsiveness, and adaptability.
A group of genetically identical cells all descended from a single common ancestral cell by mitosis in eukaryotes or by binary fission in prokaryotes. Clone cells also include populations of recombinant DNA molecules all carrying the same inserted sequence. (From King & Stansfield, Dictionary of Genetics, 4th ed)
The outward appearance of the individual. It is the product of interactions between genes, and between the GENOTYPE and the environment.
An anti-inflammatory 9-fluoro-glucocorticoid.
Studies which start with the identification of persons with a disease of interest and a control (comparison, referent) group without the disease. The relationship of an attribute to the disease is examined by comparing diseased and non-diseased persons with regard to the frequency or levels of the attribute in each group.
A chronic systemic disease, primarily of the joints, marked by inflammatory changes in the synovial membranes and articular structures, widespread fibrinoid degeneration of the collagen fibers in mesenchymal tissues, and by atrophy and rarefaction of bony structures. Etiology is unknown, but autoimmune mechanisms have been implicated.
A deoxyribonucleotide polymer that is the primary genetic material of all cells. Eukaryotic and prokaryotic organisms normally contain DNA in a double-stranded state, yet several important biological processes transiently involve single-stranded regions. DNA, which consists of a polysugar-phosphate backbone possessing projections of purines (adenine and guanine) and pyrimidines (thymine and cytosine), forms a double helix that is held together by hydrogen bonds between these purines and pyrimidines (adenine to thymine and guanine to cytosine).
Immunized T-lymphocytes which can directly destroy appropriate target cells. These cytotoxic lymphocytes may be generated in vitro in mixed lymphocyte cultures (MLC), in vivo during a graft-versus-host (GVH) reaction, or after immunization with an allograft, tumor cell or virally transformed or chemically modified target cell. The lytic phenomenon is sometimes referred to as cell-mediated lympholysis (CML). These CD8-positive cells are distinct from NATURAL KILLER CELLS and NATURAL KILLER T-CELLS. There are two effector phenotypes: TC1 and TC2.
A low affinity interleukin-11 receptor subunit that combines with the CYTOKINE RECEPTOR GP130 to form a high affinity receptor for INTERLEUKIN-11. Multiple isoforms of this protein exist due to ALTERNATIVE SPLICING of its MRNA.
A factor synthesized in a wide variety of tissues. It acts synergistically with TGF-alpha in inducing phenotypic transformation and can also act as a negative autocrine growth factor. TGF-beta has a potential role in embryonal development, cellular differentiation, hormone secretion, and immune function. TGF-beta is found mostly as homodimer forms of separate gene products TGF-beta1, TGF-beta2 or TGF-beta3. Heterodimers composed of TGF-beta1 and 2 (TGF-beta1.2) or of TGF-beta2 and 3 (TGF-beta2.3) have been isolated. The TGF-beta proteins are synthesized as precursor proteins.
The specific failure of a normally responsive individual to make an immune response to a known antigen. It results from previous contact with the antigen by an immunologically immature individual (fetus or neonate) or by an adult exposed to extreme high-dose or low-dose antigen, or by exposure to radiation, antimetabolites, antilymphocytic serum, etc.
A cytokine produced by activated T-LYMPHOCYTES that stimulates the migration of CD4-POSITIVE LYMPHOCYTES and monocytes. It has been reported to suppress HIV replication.
Detection of RNA that has been electrophoretically separated and immobilized by blotting on nitrocellulose or other type of paper or nylon membrane followed by hybridization with labeled NUCLEIC ACID PROBES.
The number of WHITE BLOOD CELLS per unit volume in venous BLOOD. A differential leukocyte count measures the relative numbers of the different types of white cells.
Substances that augment, stimulate, activate, potentiate, or modulate the immune response at either the cellular or humoral level. The classical agents (Freund's adjuvant, BCG, Corynebacterium parvum, et al.) contain bacterial antigens. Some are endogenous (e.g., histamine, interferon, transfer factor, tuftsin, interleukin-1). Their mode of action is either non-specific, resulting in increased immune responsiveness to a wide variety of antigens, or antigen-specific, i.e., affecting a restricted type of immune response to a narrow group of antigens. The therapeutic efficacy of many biological response modifiers is related to their antigen-specific immunoadjuvanticity.
Process of classifying cells of the immune system based on structural and functional differences. The process is commonly used to analyze and sort T-lymphocytes into subsets based on CD antigens by the technique of flow cytometry.
An early local inflammatory reaction to insult or injury that consists of fever, an increase in inflammatory humoral factors, and an increased synthesis by hepatocytes of a number of proteins or glycoproteins usually found in the plasma.
An INTERLEUKIN-6 related cytokine that exhibits pleiotrophic effects on many physiological systems that involve cell proliferation, differentiation, and survival. Leukemia inhibitory factor binds to and acts through the lif receptor.
A technique of culturing mixed cell types in vitro to allow their synergistic or antagonistic interactions, such as on CELL DIFFERENTIATION or APOPTOSIS. Coculture can be of different types of cells, tissues, or organs from normal or disease states.
A cell-surface ligand involved in leukocyte adhesion and inflammation. Its production is induced by gamma-interferon and it is required for neutrophil migration into inflamed tissue.
Cells that line the inner and outer surfaces of the body by forming cellular layers (EPITHELIUM) or masses. Epithelial cells lining the SKIN; the MOUTH; the NOSE; and the ANAL CANAL derive from ectoderm; those lining the RESPIRATORY SYSTEM and the DIGESTIVE SYSTEM derive from endoderm; others (CARDIOVASCULAR SYSTEM and LYMPHATIC SYSTEM) derive from mesoderm. Epithelial cells can be classified mainly by cell shape and function into squamous, glandular and transitional epithelial cells.
Diffusible gene products that act on homologous or heterologous molecules of viral or cellular DNA to regulate the expression of proteins.
Protein kinases that catalyze the PHOSPHORYLATION of TYROSINE residues in proteins with ATP or other nucleotides as phosphate donors.
Lining of the INTESTINES, consisting of an inner EPITHELIUM, a middle LAMINA PROPRIA, and an outer MUSCULARIS MUCOSAE. In the SMALL INTESTINE, the mucosa is characterized by a series of folds and abundance of absorptive cells (ENTEROCYTES) with MICROVILLI.
Recombinant proteins produced by the GENETIC TRANSLATION of fused genes formed by the combination of NUCLEIC ACID REGULATORY SEQUENCES of one or more genes with the protein coding sequences of one or more genes.
An interleukin receptor subunit that was originally discovered as a component of the INTERLEUKIN 2 RECEPTOR. It was subsequently found to be a component of several other receptors including the INTERLEUKIN 4 RECEPTOR, the INTERLEUKIN 7 RECEPTOR, the INTERLEUKIN-9 RECEPTOR, the INTERLEUKIN-15 RECEPTOR, and the INTERLEUKIN-21 RECEPTOR. Mutations in the gene for the interleukin receptor common gamma chain have been associated with X-LINKED COMBINED IMMUNODEFICIENCY DISEASES.
The major immunoglobulin isotype class in normal human serum. There are several isotype subclasses of IgG, for example, IgG1, IgG2A, and IgG2B.
Mononuclear phagocytes derived from bone marrow precursors but resident in the peritoneum.
A pattern recognition receptor that interacts with LYMPHOCYTE ANTIGEN 96 and LIPOPOLYSACCHARIDES. It mediates cellular responses to GRAM-NEGATIVE BACTERIA.
All of the processes involved in increasing CELL NUMBER including CELL DIVISION.
An albumin obtained from the white of eggs. It is a member of the serpin superfamily.
They are oval or bean shaped bodies (1 - 30 mm in diameter) located along the lymphatic system.
Toxins closely associated with the living cytoplasm or cell wall of certain microorganisms, which do not readily diffuse into the culture medium, but are released upon lysis of the cells.
Glycoprotein molecules on the surface of B- and T-lymphocytes, that react with molecules of antilymphocyte sera, lectins, and other agents which induce blast transformation of lymphocytes.
Granulated cells that are found in almost all tissues, most abundantly in the skin and the gastrointestinal tract. Like the BASOPHILS, mast cells contain large amounts of HISTAMINE and HEPARIN. Unlike basophils, mast cells normally remain in the tissues and do not circulate in the blood. Mast cells, derived from the bone marrow stem cells, are regulated by the STEM CELL FACTOR.
CD4-positive T cells that inhibit immunopathology or autoimmune disease in vivo. They inhibit the immune response by influencing the activity of other cell types. Regulatory T-cells include naturally occurring CD4+CD25+ cells, IL-10 secreting Tr1 cells, and Th3 cells.
A Janus kinase subtype that is predominantly expressed in hematopoietic cell. It is involved in signaling from a broad variety of CYTOKINE RECEPTORS including ones that utilize the INTERLEUKIN RECEPTOR COMMON GAMMA SUBUNIT.
Conversion of an inactive form of an enzyme to one possessing metabolic activity. It includes 1, activation by ions (activators); 2, activation by cofactors (coenzymes); and 3, conversion of an enzyme precursor (proenzyme or zymogen) to an active enzyme.
A cell surface receptor that specifically mediates the biological effects of INTERLEUKIN-9. The functional IL9 receptor signals through interaction of its cytoplasm domain with JANUS KINASES and requires the INTERLEUKIN RECEPTOR COMMON GAMMA SUBUNIT for activity.
Proteins that are secreted into the blood in increased or decreased quantities by hepatocytes in response to trauma, inflammation, or disease. These proteins can serve as inhibitors or mediators of the inflammatory processes. Certain acute-phase proteins have been used to diagnose and follow the course of diseases or as tumor markers.
A classification of lymphocytes based on structurally or functionally different populations of cells.
An interleukin-1 receptor subtype that competes with the INTERLEUKIN-1 RECEPTOR TYPE I for binding to INTERLEUKIN-1ALPHA and INTERLEUKIN-1BETA. The interleukin-1 type II receptor appears to lack signal transduction capability. Therefore it may act as a "decoy" receptor that modulates the activity of its ligands. Both membrane-bound and soluble forms of the receptor have been identified.
White blood cells. These include granular leukocytes (BASOPHILS; EOSINOPHILS; and NEUTROPHILS) as well as non-granular leukocytes (LYMPHOCYTES and MONOCYTES).
Biologically active substances whose activities affect or play a role in the functioning of the immune system.
Differentiation antigens found on thymocytes and on cytotoxic and suppressor T-lymphocytes. CD8 antigens are members of the immunoglobulin supergene family and are associative recognition elements in MHC (Major Histocompatibility Complex) Class I-restricted interactions.
A cytokine with both pro- and anti-inflammatory actions that depend upon the cellular microenvironment. Oncostatin M is a 28 kDa monomeric glycoprotein that is similar in structure to LEUKEMIA INHIBITORY FACTOR. Its name derives from the the observation that it inhibited the growth of tumor cells and augmented the growth of normal fibroblasts.
A family of pattern recognition receptors characterized by an extracellular leucine-rich domain and a cytoplasmic domain that share homology with the INTERLEUKIN 1 RECEPTOR and the DROSOPHILA toll protein. Following pathogen recognition, toll-like receptors recruit and activate a variety of SIGNAL TRANSDUCING ADAPTOR PROTEINS.
Culture media containing biologically active components obtained from previously cultured cells or tissues that have released into the media substances affecting certain cell functions (e.g., growth, lysis).
Mice homozygous for the mutant autosomal recessive gene "scid" which is located on the centromeric end of chromosome 16. These mice lack mature, functional lymphocytes and are thus highly susceptible to lethal opportunistic infections if not chronically treated with antibiotics. The lack of B- and T-cell immunity resembles severe combined immunodeficiency (SCID) syndrome in human infants. SCID mice are useful as animal models since they are receptive to implantation of a human immune system producing SCID-human (SCID-hu) hematochimeric mice.
The soft tissue filling the cavities of bones. Bone marrow exists in two types, yellow and red. Yellow marrow is found in the large cavities of large bones and consists mostly of fat cells and a few primitive blood cells. Red marrow is a hematopoietic tissue and is the site of production of erythrocytes and granular leukocytes. Bone marrow is made up of a framework of connective tissue containing branching fibers with the frame being filled with marrow cells.
Cell surface receptors that bind TUMOR NECROSIS FACTORS and trigger changes which influence the behavior of cells.
A single, unpaired primary lymphoid organ situated in the MEDIASTINUM, extending superiorly into the neck to the lower edge of the THYROID GLAND and inferiorly to the fourth costal cartilage. It is necessary for normal development of immunologic function early in life. By puberty, it begins to involute and much of the tissue is replaced by fat.
A specific immune response elicited by a specific dose of an immunologically active substance or cell in an organism, tissue, or cell.
A large lobed glandular organ in the abdomen of vertebrates that is responsible for detoxification, metabolism, synthesis and storage of various substances.

Systemic administration of rIL-12 synergistically enhances the therapeutic effect of a TNF gene-transduced cancer vaccine. (1/4931)

Interleukin-12 (IL-12) is a potent antitumor cytokine, which induces and enhances the activity of natural killer (NK) cells, lymphokine activated killer (LAK) cells and cytotoxic T lymphocytes (CTL). IL-12 also stimulates IFN-gamma production from both T cells and NK cells. In this study, we transfected methylcholanthrene-induced fibrosarcoma (MCA-D) with TNF gene and investigated the therapeutic effect of TNF gene-transduced cancer vaccine and whether the vaccination effect is enhanced by systemic administration of recombinant IL-12 (rIL-12), in a murine model. TNF gene-transduced cancer vaccine or systemic administration of rIL-12 showed slight or moderate inhibition of pre-established tumor. However, simultaneous application of the vaccine and rIL-12 resulted in complete eradication. The cytotoxicity of CTL against parental tumor cells was enhanced with the combination of the vaccine and rIL-12, and IFN-gamma production from spleen cells also increased synergistically. Our findings show that synergistic enhancement of CTL activity and IFN-gamma production could play an important role in the antitumor effect of combination therapy using TNF gene-transduced cancer vaccine and rIL-12.  (+info)

Reciprocal control of T helper cell and dendritic cell differentiation. (2/4931)

It is not known whether subsets of dendritic cells provide different cytokine microenvironments that determine the differentiation of either type-1 T helper (TH1) or TH2 cells. Human monocyte (pDC1)-derived dendritic cells (DC1) were found to induce TH1 differentiation, whereas dendritic cells (DC2) derived from CD4+CD3-CD11c- plasmacytoid cells (pDC2) induced TH2 differentiation by use of a mechanism unaffected by interleukin-4 (IL-4) or IL-12. The TH2 cytokine IL-4 enhanced DC1 maturation and killed pDC2, an effect potentiated by IL-10 but blocked by CD40 ligand and interferon-gamma. Thus, a negative feedback loop from the mature T helper cells may selectively inhibit prolonged TH1 or TH2 responses by regulating survival of the appropriate dendritic cell subset.  (+info)

Enhanced Th1 and dampened Th2 responses synergize to inhibit acute granulomatous and fibrotic responses in murine schistosomiasis mansoni. (3/4931)

In murine schistosomiasis mansoni, CD4(+) Th1 and Th2 cells participate in the ovum-induced granulomatous inflammation. Previous studies showed that the interleukin-12 (IL-12)-induced Th1 response strongly suppressed the Th2-cell-mediated pulmonary granuloma development in naive or primed mice. However, liver granulomas were only moderately suppressed in egg-vaccinated, recombinant IL-12 (rIL-12)-treated infected mice. The present study shows that repeated rIL-12 injections given during early granuloma development at 5 to 7 weeks after infection prolonged the Th1 phase and resulted in gamma interferon-mediated suppression of liver granulomas. The timing is crucial: if given at 6 to 8 weeks, during the Th2-dominated phase of florid granuloma growth, the treatment is ineffective. Daily injections of rIL-12 given between 5 and 7.5 weeks during the period of granuloma growth achieved a somewhat-stronger diminution in granuloma growth with less deposition of collagen but caused 60% mortality and liver pathology. In contrast, combined treatment with rIL-12 and anti-IL-4-anti-IL-10 monoclonal antibody (MAb) injections given during the Th2 phase strongly inhibited liver granuloma growth without mortality. The diminished inflammatory response was accompanied by less deposition of collagen in the liver. Moreover, neutralization of endogenous IL-12 by anti-IL-12 MAbs effectively decreased the early Th1 phase (between 5 and 6 weeks after infection) but not the developing Th2 phase (5 to 7 weeks) of granuloma development. These studies indicate that the granulomatous response in infected mice can be manipulated by utilizing the Th1-Th2-subset antagonism with potential salutary results in the amelioration of fibrous pathology.  (+info)

Interleukin-12 is synthesized by mesangial cells and stimulates platelet-activating factor synthesis, cytoskeletal reorganization, and cell shape change. (4/4931)

Preliminary studies indicate the involvement of interleukin (IL)-12 in experimental renal pathology. In the present study, we evaluated whether cultured glomerular mesangial cells are able to produce IL-12 and whether IL-12 may regulate some of their functions, including the cytoskeletal reorganization, the change in cell shape, and the production of platelet-activating factor (PAF). The results obtained indicate that pro-inflammatory stimuli, such as tumor necrosis factor-alpha and bacterial polysaccharides, induce the expression of IL-12 mRNA and the synthesis of the protein by cultured mesangial cells. Moreover, cultured mesangial cells were shown to bind IL-12 and to express the human low-affinity IL-12 beta1-chain receptor. When challenged with IL-12, mesangial cells produced PAF in a dose- and time-dependent manner and superoxide anions. No production of tumor necrosis factor-alpha and IL-8 was observed. Moreover, we demonstrate that IL-12 induced a delayed and sustained shape change of mesangial cells that reached its maximum between 90 and 120 minutes of incubation. The changes in cell shape occurred concomitantly with cytoskeletal rearrangements and may be consistent with cell contraction. As IL-12-dependent shape change of mesangial cells was concomitant with the synthesis of PAF, which is known to promote mesangial cell contraction, we investigated the role of PAF using two chemically different PAF receptor antagonists. Both antagonists inhibited almost completely the cell shape change induced by IL-12, whereas they were ineffective on angiotensin-II-induced cell shape change. In conclusion, our results suggest that mesangial cells can either produce IL-12 or be stimulated by this cytokine to synthesize PAF and to undergo shape changes compatible with cell contraction.  (+info)

Downregulation of interleukin-12 (IL-12) responsiveness in human T cells by transforming growth factor-beta: relationship with IL-12 signaling. (5/4931)

Interleukin-12 (IL-12) is a cytokine that plays a central role in the control of cell-mediated immunity. We have previously shown that transforming growth factor-beta1 (TGF-beta) inhibitory effects on human primary allogeneic cytotoxicity and proliferative responses interfere with IL-12 pathway. The present study was undertaken to further elucidate the biochemical basis of the functional interaction between these two cytokines and to define the site of TGF-beta action on the signaling pathway activated by IL-12. Our data indicate that TGF-beta induced an inhibition of interferon-gamma (IFN-gamma) production without affecting the IL-12Rbeta1 and IL-12Rbeta2 subunits mRNA expression by activated T cells. We further show that TGF-beta has a significant inhibitory effect on the early signal transduction events following interaction of IL-12 with its receptor on activated T cells, resulting in the inhibition of both JAK2 and Tyk2 phosphorylation. In addition, TGF-beta was found to significantly inhibit IL-12-induced phosphorylation of the STAT4 transcription factor. Electrophoretic mobility shift assay indicated that TGF-beta induced a decrease in IL-12-induced STAT4 DNA binding activity in T lymphocytes. This study suggests that TGF-beta influences IL-12 responsiveness at least in part by inhibiting early signaling events essential to gene induction in IL-12-activated T cells.  (+info)

Contribution of natural killer cells to inhibition of angiogenesis by interleukin-12. (6/4931)

Interleukin-12 (IL-12) inhibits angiogenesis in vivo by inducing interferon-gamma (IFN-gamma) and other downstream mediators. Here, we report that neutralization of natural killer (NK) cell function with antibodies to either asialo GM1 or NK 1.1 reversed IL-12 inhibition of basic fibroblast growth factor (bFGF)-induced angiogenesis in athymic mice. By immunohistochemistry, those sites where bFGF-induced neovascularization was inhibited by IL-12 displayed accumulation of NK cells and the presence of IP-10-positive cells. Based on expression of the cytolytic mediators perforin and granzyme B, the NK cells were locally activated. Experimental Burkitt lymphomas treated locally with IL-12 displayed tumor tissue necrosis, vascular damage, and NK-cell infiltration surrounding small vessels. After activation in vitro with IL-12, NK cells from nude mice became strongly cytotoxic for primary cultures of syngeneic aortic endothelial cells. Cytotoxicity was neutralized by antibodies to IFN-gamma. These results document that NK cells are required mediators of angiogenesis inhibition by IL-12, and provide evidence that NK-cell cytotoxicity of endothelial cells is a potential mechanism by which IL-12 can suppress neovascularization.  (+info)

Interleukin-12 induces expression of interferon regulatory factor-1 via signal transducer and activator of transcription-4 in human T helper type 1 cells. (7/4931)

IRF-1-deficient mice show a striking defect in the development of T helper 1 (Th1) cells. In the present report, we investigate the expression of IRF-1 during differentiation of human T helper cells. No significant differences of IRF-1 mRNA expression were found in established Th1 and Th2 cells; however, interleukin 12 (IL-12) induced a strong up-regulation of IRF-1 transcripts in Th1 but not in Th2 cells. We demonstrate that IL-12-induced up-regulation of IRF-1 is mediated by signal transducer and activator of transcription-4, which binds to the interferon (IFN)-gamma-activated sequence present in the promoter of the IRF-1 gene. Strong IL-12-dependent activation of a reporter gene construct containing the IRF-1 IFN-gamma-activated sequence element provides further evidence for the key role of signal transducer and activator of transcription-4 in the IL-12-induced up-regulation of IRF-1 transcripts in T cells. IRF-1 expression was strongly induced after stimulation of naive CD4(+) T cells via the T cell receptor, irrespective of the cytokines present at priming, indicating that this transcription factor does not play a major role in initiating a Th1-specific transcriptional cascade in differentiating helper T cells. However, our finding that IRF-1 is a target gene of IL-12 suggests that some of the IL-12-induced effector functions of Th1 cells may be mediated by IRF-1.  (+info)

The vitronectin receptor and its associated CD47 molecule mediates proinflammatory cytokine synthesis in human monocytes by interaction with soluble CD23. (8/4931)

The vitronectin receptor, alphavbeta3 integrin, plays an important role in tumor cell invasion, angiogenesis, and phagocytosis of apoptotic cells. CD47, a member of the multispan transmembrane receptor family, physically and functionally associates with vitronectin receptor (VnR). Although vitronectin (Vn) is not a ligand of CD47, anti-CD47 and beta3 mAbs suppress Vn, but not fibronectin (Fn) binding and function. Here, we show that anti-CD47, anti-beta3 mAb and Vn, but not Fn, inhibit sCD23-mediated proinflammatory function (TNF-alpha, IL-12, and IFN-gamma release). Surprisingly, anti-CD47 and beta3 mAbs do not block sCD23 binding to alphav+beta3+ T cell lines, whereas Vn and an alphav mAb (clone AMF7) do inhibit sCD23 binding, suggesting the VnR complex may be a functional receptor for sCD23. sCD23 directly binds alphav+beta3+/CD47(-) cell lines, but coexpression of CD47 increases binding. Moreover, sCD23 binds purified alphav protein and a single human alphav chain CHO transfectant. We conclude that the VnR and its associated CD47 molecule may function as a novel receptor for sCD23 to mediate its proinflammatory activity and, as such, may be involved in the inflammatory process of the immune response.  (+info)

TY - JOUR. T1 - Reversal of defective IL-6 production in lipopolysaccharide-tolerant mice by phorbol myristate acetate. AU - Mengozzi, Manuela. AU - Sironi, Marina. AU - Gadina, Massimo. AU - Ghezzi, Pietro. PY - 1991/8/1. Y1 - 1991/8/1. N2 - The development of LPS tolerance has been suggested to be mediated by an inhibition of cytokine synthesis. Here we have studied serum IL-6 and TNF levels in mice after LPS administration. Repeated administration of LPS (35 μg daily for 4 days) to mice induced a refractoriness (tolerance) to subsequent administrations of LPS in terms of induction of circulating IL-6 and TNF. To investigate the mechanism by which LPS down-regulates its own induction of cytokine synthesis and the relationship between IL-6 and TNF production, we attempted to revert the inhibition of IL-6 and TNF production using agents like PMA or IFN-γ, previously reported to activate macrophage production of cytokines. Pretreatment with PMA (4 μg, 10 min before LPS) partially restored IL-6 ...
Description - Interleukin-27 (IL-27), a heterodimeric cytokine and a relatively new member of the IL-6/IL-12 family, plays an important role in the regulation of Th1 responses. IL-27 consists of two subunits, an EBV-transformed gene 3 (EBI3) subunit and a p28 cytokine subunit. IL-27 activity is mediated by binding to its receptor, IL-27R, comprised of WSX-1 and gp130. Several immune cells co-express both subunits of the IL-27 receptor. IL-27 is expressed by antigen-presenting cells upon antigen activation. IL-27 potently induces the proliferation of naive T cells, and synergistically with IL-12 stimulates the production of IFN-γ by naive CD4+ T cells ...
Harris, S. J., Ciuclan, L., Finan, P. M., Wymann, M. P., Walker, C., Westwick, J., Ward, S. G. and Thomas, M. J., 2012. Genetic ablation of PI3Kγ results in defective IL-17RA signalling in T lymphocytes and increased IL-17 levels. European Journal of Immunology ...
reference: Charged residues dominate a unique interlocking topography in the heterodimeric cytokine interleukin-12., Yoon C, Johnston SC, Tang J, Stahl M, Tobin JF, Somers WS, EMBO J 2000 Jul 17;19(14):3530-41. PMID: 10899108 ...
IL-18 (also known as IFN-gamma-inducing factor), although structurally unrelated to IL-12, shares with it the role of activating NK cells and polarizing T cells toward Th1 cell function. To understand how the IL-18 gene (and consequently Th1 function) is regulated, we have determined the gene structure and investigated the mechanisms of transcriptional control and cell type expression. The mouse IL-18 gene comprises seven exons distributed over 26 kb. Exons 1 and 2 of this gene are 5-noncoding exons. Promoter activity was detected upstream of these noncoding exons in two distinct regions. Both promoters are TATA-less and not G+C rich. The promoter activity located upstream of exon 2 was shown to act constitutively, while the activity located upstream of exon 1 was up-regulated in activated macrophage and T cell lines. IL-18 gene expression may be regulated in a wide range of cell types by the activities of these two distinct promoters. IL-18 is known to be synthesized as a precursor, pro-IL-18, and its
The G23-8 monoclonal antibody reacts with the p19 subunit of mouse IL-23. IL-23 is a heterodimeric cytokine composed of two disulfide-linked subunits, a p19 subunit that is unique to IL-23, and a p40 subunit that is shared with IL-12. IL-23 is secreted by activated dendritic cells and macrophages. IL-23 has been shown to enhance IFNγ production by memory T cells. Additionally, mouse IL-23 induces the proliferation of memory T cells (but not naive T cells), whereas IL-12 has no effect on memory cells. Mouse IL-23 (but not IL-12) can also activate mouse memory T cells to produce the potent proinflammatory cytokine IL-17. IL-23 has been shown to be upregulated in certain autoimmune diseases and promote immunity in response to some viral and mycobacterial infections. The G23-8 antibody can specifically neutralize IL-23 bioactivity with no effect on IL-12 p70 bioactivity.
This gene encodes a subunit of the heterodimeric cytokine interleukin 23 (IL23). IL23 is composed of this protein and the p40 subunit of interleukin 12 (IL12B). The receptor of IL23 is formed by the beta 1 subunit of IL12 (IL12RB1) and an IL23 specific subunit, IL23R. Both IL23 and IL12 can activate the transcription activator STAT4, and stimulate the production of interferon-gamma (IFNG). In contrast to IL12, which acts mainly on naive CD4(+) T cells, IL23 preferentially acts on memory CD4(+) T cells ...
IL18 (IFN-gamma-inducing factor, IGIF) is a novel pro-inflammatory cytokine that augments natural killer (NK) activity in spleen cells.This cytokine…
Does anybody know how much total protein there is in human dendritic cells per cell? I am interested in all of these three DC subtypes ...
IL-2-regulated genes in PBMCs. Pre-activated, rested human PBMCs were left untreated or restimulated for 4 hr with IL-2, and RNA probes were prepared for hybrid
In this study, we show that human IL-17F/IL-17A heterodimer, the recently identified member of the IL-17 cytokine family, utilizes the same receptor complex as the IL-17F and IL-17A cytokines. Using various experimental approaches, including surface plasmon resonance and siRNA gene knockdown, we characterized the physical and functional interactions of IL-17F/IL-17A, IL-17F, and IL-17A with the IL-17RA and IL-17RC receptors and propose that all three cytokines require both receptors for their biological activity.. Toy et al. demonstrated that human IL-17A or IL-17F could not induce CXCL1 expression in IL-17RA−/− fibroblast cells and that transfection of human IL-17RA did not rescue the expression of CXCL1 as was seen in wild-type cells (26). When the cells were cotransfected with both human IL-17RA and IL-17RC and treated with either human IL-17A or IL-17F, production of CXCL1 was restored, suggesting that a heterodimeric IL-17RA/IL-17RC receptor was required for signaling (26). Recently, it ...
Invitrogen™ eBioscience™ Mouse IL-6 ELISA Ready-SET-Go!™ Kit 2 x 96 tests Invitrogen™ eBioscience™ Mouse IL-6 ELISA...
Tedder, T. F. and Jansen, P. J. 2001. Isolation and Generation of Human Dendritic Cells. Current Protocols in Immunology. 23:IV:7.32:7.32.1-7.32.16. ...
摘 要:γδT细胞是一群异质细胞,人和小鼠不同组织部位的γδT细胞亚群表型多变、功能丰富。γδT细胞经过胸腺选择,形成IL-17+γδT细胞、IFN-γ+γδT细胞或IL-4+γδT细胞,它的分化受到很多因素的调控。γδT细胞是炎症介质IL-17的重要来源,IL-17+γδT细胞可以参与多种疾病的诱发和发展,如过敏、自身免疫性疾病,甚至恶性肿瘤。此外,它们也在宿主防御中发挥保护作用,防治传染病和诱导细胞毒性T淋巴细胞对癌症的反应。就IL-17+γδT细胞的发育、分化和调节机制以及在各种疾病中的作用进行综述 ...
有多种机制认为病毒感染与过敏性炎症相互作用,从而导致下呼吸道功能障碍、喘息和哮喘。首先,潜在的过敏性炎症可以直接增强气道对鼻病毒感染的反应性。此外,病毒感染可损害气道上皮的屏障功能,导致气道壁对气传过敏原的吸收增加和炎症反应增强,而潜在的过敏性炎症也可能导致病毒复制增强。值得注意的是,鼻病毒感染和变应原均可促进气道上皮细胞产生IL-33, IL-33是最近发现的一种先天细胞因子,可促进2型气道炎症和重塑。据报道,这种类固醇耐药途径在难以控制哮喘的儿童中上调。有趣的是,IL-33多聚物与中晚期发作的喘息有关,而中晚期发作的喘息与早期生活中的过敏反应密切相关。 另一种先天上皮细胞因子IL-25也由鼻病毒诱导,在过敏患者鼻病毒感染的情况下,IL-25可能加重过敏性气道炎症 ...
Инфламмасома - важный компонент нативного иммунитета. Она представляет собой макромолекулярный комплекс, включающий сенсорные элементы, адапторные белки и зимоген каспазы-1. Под действием продуктов распада тканей и патогенных микроорганизмов инфламмасома активируется и превращает про-IL-1b и про-IL-18 в активные интерлейкины. Активация инфламмасом отмечена при многих воспалительных заболеваниях и служит мишенью для терапевтических воздействий. В настоящем обзоре обсуждается вклад инфламмасом в патогенез социально-значимых заболеваний ...
货号产品说明规格方法学认证4300604Vialshaker(IKA Vibrax300rpm) 1pceRIAInstruments4300606Vialextraction insertforurinaryhGH1pceRIAInstrumentsKIC1261IL-6 96TIRMARUOKIC1
TY - JOUR. T1 - Morphine inhibits murine dendritic cell IL-23 production by modulating toll-like receptor 2 and Nod2 signaling. AU - Wang, Jinghua. AU - Ma, Jing. AU - Charboneau, Rick. AU - Barke, Roderick. AU - Roy, Sabita. PY - 2011/3/25. Y1 - 2011/3/25. N2 - IL-23, produced by dendritic cells (DCs) and macrophages, plays a critical role in innate immunity against bacterial infection. Our previous studies show that morphine disrupts the IL-23/IL-17 mediated pulmonary mucosal host defense and increases susceptibility to Streptococcus pneumoniae lung infection. To determine the mechanism by which morphine modulates IL-23 production, mouse bone marrow-derived dendritic cells (BMDCs) and macrophages (BMDMs) were treated with morphine, and infected with S. pneumoniae or stimulated with Toll-like receptor (TLR) and Nod2 ligands. We found that a significant increase in IL-23 protein production was observed in S. pneumoniae, TLR2 ligand lipoteichoic acid (LTA), and TLR4 ligand pneumolysin (PLY) ...
T cell stimulatory capacity of DCs cultured with PGE2. Day-5 DCs were recultured in the absence or presence of PGE2 (10 μM), TNF-α (1,000 U/ml), or PGE2 pl
Interleukin-23 (IL-23) is a heterodimeric cytokine composed of an IL12B (IL-12p40) subunit (that is shared with IL12) and the IL23A (IL-23p19) subunit. A functional receptor for IL-23 (the IL-23 receptor) has been identified and is composed of IL-12R β1 and IL-23R. IL-23 was first described by Robert Kastelein and colleagues at the DNAX research institute using a combination of computational, biochemical and cellular immunology approaches. Prior to the discovery of IL-23, IL-12 had been proposed to represent a key mediator of inflammation in mouse models of inflammation. However, many studies aimed at assessing the role of IL-12 had blocked the activity of IL-12p40, and were therefore not as specific as thought. Studies which blocked the function of IL-12p35 did not produce the same results as those targeting IL-12p40 as would have been expected if both subunits formed part of IL-12 only. The discovery of an additional potential binding partner for IL-12p40 led to a reassessment of this role ...
A correction to this article has been published and is linked from the HTML version of this paper. The error has not been fixed in the paper.
CCL4, CCL20, IFN-γ, IL-2, IL-4, IL-10, IL-16, IL-17A, IL-17F, IL-18, MIF, TNSF15, IL-1β, IL-1R, IL-6, IL-7, IL-7R, IL-8, IL-10R, IL-12p35, IL-12p40, IL-3, IL-15, IL-16, IL-21, IL-21R, IL-22, IL-17D, LITAF, NK-lysin, CD25, CD80, CD83, CD86, IFN-α, IFN-r, TGFB4, B-defensin8 ...
Interleukin-6 (IL-6), 0.5 mg. IL-6 is a potent lymphoid cell growth factor that stimulates the growth and survival of certain B cells and T cells.
IL-12/IL-23 p40, Functional Grade, clone: C8.6, eBioscience™ 500μg; Functional Grade IL-12/IL-23 p40, Functional Grade, clone: C8.6, eBioscience™...
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This Application describes a reliable and reproducible protocol for the generation of monocyte-derived dendritic cells (Mo-DCs). - USA
TABLE-US-00002 TABLE 2 Relative expression of interferon-gamma from human NK cells, as determined by Taqman ® real time PCR. Relative Condition expression NK media only 1 1:625 IL-27 1 1:125 IL-27 1 1:25 IL-27 1 1:5 IL-27 1 100 ng/ml IL-27 1 IL-2 only (100 ng/ml) 320 IL-27 + 1:625 IL-2 120 IL-27 + 1:125 IL-2 240 IL-27 + 1:25 IL-2 340 IL-27 + 1:5 IL-2 310 IL-27 + IL-2 (100 ng/ml) 190 IL-12 only (100 ng/ml) 650 IL-27 + 1:625 IL-12 740 IL-27 + 1:125 IL-12 710 IL-27 + 1:25 IL-12 690 IL-27 + 1:5 IL-12 705 IL-27 + IL-12 (100 ng/ml) 820 IL-18 only 1 IL-27 + 1:625 IL-18 1 IL-27 + 1:125 IL-18 1 IL-27 + 1:25 IL-18 1 IL-27 + 1:5 IL-18 1 IL-27 + IL-18 (100 ng/ml) 1 IL-2 + IL-12 2800 IL-27 + 1:625 IL-2 + IL-12 1500 IL-27 + 1:125 IL-2 + IL-12 1600 IL-27 + 1:25 IL-2 + IL-12 1700 IL-27 + 1:5 IL-2 + IL-12 2600 IL-27 + IL-2 (100 ng/ml) + IL-12 2100 IL-2 + IL-18 500 IL-27 + 1:625 IL-2 + IL-18 300 IL-27 + 1:125 IL-2 + IL-18 300 IL-27 + 1:25 IL-2 + IL-18 600 IL-27 + 1:5 IL-2 + IL-18 500 IL-27 + IL-2 (100 ng/ml) + ...
A murine cell line (IxN/2b) absolutely dependent upon exogenous IL-7 for continued growth has been obtained that expresses lymphoid precursor and class I MHC antigens and also contains a rearranged mu heavy chain. This cell line has been used to define the binding and structural characteristics of the murine IL-7 receptor using 125I-labeled recombinant murine IL-7. 125I-IL-7 binding to IxN/2b cell was rapid and saturable at both 4 degrees and 37 degrees C. Equilibrium binding studies produced curvilinear Scatchard plots at both temperatures with high and low affinity Ka values of approximately 1 x 10(10) M-1 and 4 x 10(8) M-1, respectively, and a total of 2,000-2,500 IL-7 binding sites expressed per cell. Experiments measuring inhibition of binding of 125I-IL-7 by unlabeled IL-7 also produced data consistent with the existence of two classes of IL-7 receptors. Evidence concerning the possible molecular nature of two classes of IL-7 receptors was provided by dissociation kinetics and affinity ...
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The Murine Dendritic Cell Cytokine Package contains the key components required for the culturing of your mouse dendritic cells.|/p| The package
NEX232 Interleukin-1 (IL-1) refers to a group of three polypeptides (interleukin-1 alpha (IL-1α), interleukin-1 beta (IL-1β) and interleukin-1 receptor antagonist (IL-1Ra)), that play a central role in the regulation of immune and inflammatory responses.. IL-1β is a pro-inflammatory cytokine involved in immune defense against infection. ...
Caruso, Denise A, Orme, Lisa M, Neale, Alana M, Radcliff, Fiona J, Amor, Gerlinda M, Maixner, Wirginia, Downie, Peter, Hassall, Timothy E, Tang, Mimi LK and Ashley, David M 2004, Results of a phase 1 study utilizing monocyte-derived dendritic cells pulsed with tumor RNA in children and young adults with brain cancer, Neuro-oncology, vol. 6, no. 3, pp. 236-246, doi: 10.1215/S1152851703000668. ...
Murine IL-6 ELISA Set is intended for use in a do it yourself solid phase sandwich ELISA for the in vitro qualitative and quantitative determination of Murine IL-6 in supernatants, buffered solutions, serum, plasma samples.
人IL-17 ELISA试剂盒(Interleukin-17) ELISA试剂盒datasheet (ab100556).Abcam抗体、ELISA、激动剂拮抗剂、表观遗传试剂、蛋白多肽,使用效果保证,中国70%以上现货。
IL-23A/IL-23 P19 Proteins available through Novus Biologicals. Browse our IL-23A/IL-23 P19 Protein catalog backed by our Guarantee+.
Mouse IL-17A standard, 0.3 µg, for use in AlphaLISA immunoassays. This vial contains enough analyte to run 10 standard curves. This standard is provided in the AlphaLISA mouse IL-17A kits, but can also be ordered separately.
姑且不提這尾巴動得好猥褻 =口= a 能自動偵測感光....不是等於自動測光嗎XDDD. 那一台DC沒有的真想見識一下. 要說特別3這兩台DC只是把測光後的結果3套入這兩台相機裡預設的曲線. 讓相機替你決定光圈快門讓你不會手震後3用提高感度的方式來達到正確的曝光. 至於ISO過高會造成甚麼樣的問題3相信略懂攝影的各位朋友應該很清楚了》. 而高ISO那麼好3DC就不用裝內閃了XDDD. ...
Our results reveal that the TLR 9 ligand, CpG DNA, is the most potent signal that cooperates with CD8 T cells for the induction of IL-12p70. This is consistent with the observation that CpG DNA is a powerful stimulator of Th1 responses (38). This indicates that unmethylated CpG DNA motifs found in the genomes of bacteria and DNA viruses are a critical component for CD8 T cells to prime DC IL-12p70. The preferential ability of memory CD8 T cells to prime DCs for IL-12p70 production has important implications for the induction of cell mediated immunity during secondary immune responses. The high frequency of Ag-specific memory CD8 T cells, and their rapid recruitment to lymphoid organs or site of infection facilitates the early interaction of memory CD8 T cells with Ag-bearing DCs. Interacting memory CD8 T cells, in conjunction with TLR 9 stimulation of DCs that could occur during infection with viruses or intracellular bacteria that possess unmethylated CpG DNA within their genomes would hence ...
Downregulating galectin-3 inhibits proinflammatory cytokine production by human monocyte-derived dendritic cells via RNA interference ...
Interleukin 27 (IL-27) is a member of the IL-12 cytokine family. It is a heterodimeric cytokine that is composed of two distinct genes, Epstein-Barr virus-induced gene 3 (EBI3) and IL-27p28. IL-27 is expressed by antigen presenting cells and interacts with a specific cell-surface receptor complex known as IL-27 receptor (IL-27R). This receptor consists of two proteins, IL-27ɑ and gp130. IL-27 induces differentiation of the diverse populations of T cells in the immune system and also upregulates IL-10. When IL-27 binds to the IL-27 receptor, signaling pathways including JAK-STAT and p38 MAPK pathways are turned on. There are two types of responses, pro-inflammatory and anti-inflammatory, which involve different types of cells, such as macrophages, dendritic cells, T cells, and B cells. The response that is activated is very much dependent on the external surrounding of IL-27. There are many different subsets of T cells, such as Th1, Th2, Th17, Tr1, and Treg cells; IL-27 is greatly involved in ...
IL-1 receptor antagonist, IL-1RA/ IL-1RN, was initially purified from medium conditioned by human monocytes. IL-1RA belongs to the IL-1 family of cytokines, which includes 11 members that include three subfamilies in addition to the IL-1RA: IL-1 subfamily (IL-1α, IL-1β, IL-33), L-18 subfamily (IL-18
Human CellExp IL-27, human recombinant protein, p28, IL30, IL-27, IL-27A, IL27p28, Interleukin-27, EBI3 validated in (PBV10859r-10), Abgent
HEK-Blue™ IL-17 cells allow the colorimetric detection of bioactive human and murine interleukin-17A (IL-17A), as well as human IL-17F and IL-17E (IL-25). They can be used for the screening of anti-IL-17 antibodies or for IL-17 cell-based potency assay.
Results A total of 295 MSM participated in the survey. Almost half (weighted percentage: 47.3%) reported STI symptoms in the last 6 months and 12.9% tested HIV-positive. Prevalence of non-HIV STI was 13.5%; syphilis prevalence was 9.0%. Adjusting for age and education, STI was associated with HIV (adjusted OR (AOR)=3.46, 95% CI 1.03 to 11.64), alcohol use before sex (AOR=4.99, 95% CI 1.86 to 13.38) and having sold sex in the last 3 months (AOR=3.17, 95% CI 1.25 to 8.07), and inversely associated with having anonymous sex partners (AOR=0.20, 95% CI 0.07 to 0.61). ...
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These Lucky Cereal Bars are fun and easy recipe to make with the kids... or just for my roommates! :) I pretty much ate half the pan.
Insufficient elimination of the hepatitis C virus (HCV) during acute infection results in chronic disease in the majority of patients due to weak virus-specific immune responses. Dendritic cells (DC) play a central role in recognition of HCV and in induction of innate and adaptive immune responses. In this study, we evaluated the frequency and functions of plasmacytoid dendritic cells (PDC) and myeloid dendritic cells (MDC) in patients with chronic HCV infection. We found that both the numbers and IFNalpha production capacity of blood PDC were significantly reduced in patients with chronic HCV infection compared to normal controls. While the frequency of MDC was not affected in chronic HCV, the allostimulatory capacity of monocyte-derived MDC was significantly decreased compared to normals. Lipopolysaccharide (LPS)-induced maturation improved the allostimulatory capacity of HCV infected patients MDC that still remained significantly lower compared to normal controls. Our experiments revealed that MDC
TY - JOUR. T1 - Induction of atypical EAE mediated by transgenic production of IL-6 in astrocytes in the absence of systemic IL-6. AU - Giralt, Mercedes. AU - Ramos, Raquel. AU - Quintana, Albert. AU - Ferrer, Beatriz. AU - Erta, Maria. AU - Castro-Freire, Marco. AU - Comes, Gemma. AU - Sanz, Elisenda. AU - Unzeta, Mercedes. AU - Pifarré, Paula. AU - García, Agustina. AU - Campbell, Iain L.. AU - Hidalgo, Juan. PY - 2013/4/1. Y1 - 2013/4/1. N2 - Interleukin (IL)-6 is crucial for the induction of many murine models of autoimmunity including experimental autoimmune encephalomyelitis (EAE), an animal model of multiple sclerosis. While IL-6-deficient mice (IL-6 KO) are resistant to EAE, we showed previously that in transgenic mice with astrocyte-targeted production of IL-6-restricted to the cerebellum (GFAP-IL6), EAE induced with MOG35-55 was redirected away from the spinal cord to the cerebellum. To further establish the importance of IL-6 produced in the central nervous system, we have generated ...
TY - JOUR. T1 - Failure of exogenously administered interferon-γ or blockage of endogenous interleukin-4 with specific inhibitors to augment the incidence of autoimmune diabetes in male NOD mice. AU - Xiang, Ming. AU - Zaccone, Paola. AU - Di Marco, Roberto. AU - Harris, Robert. AU - Magro, Gaetano. AU - Di Mauro, Maurizio. AU - Meroni, Pier Luigi. AU - Garotta, Gianni. AU - Nicoletti, Ferdinando. PY - 1999. Y1 - 1999. N2 - Interferon (IFN)-γ and interleukin (IL)-4 are prototypic type 1 and type 2 cytokines which are known to play pathogenetic and protective roles, respectively, in NOD mouse IDDM. The capacity of male NOD mice to produce more IL-4 and less IFN-γ within the insulitic lesions than females has been suggested to contribute to their lower incidence of diabetes. In this study we have tested the effects of prolonged prophylactic treatment of male NOD mice with rat IFN-γ, mouse IFN-γ, anti-IL-4 monoclonal antibody (mAb) and recombinant murine soluble IL-4 receptor (smIL-4R) on the ...
In this report we describe the generation of mice deficient in IL-13Rα2 to define the role of this receptor chain in IL-13 responses. IL-13Rα2 may act to modulate the effects of IL-13 in vivo in various ways. IL-13Rα2 could enhance IL-13 activities by increasing the strength of IL-13 signaling or attenuate IL-13 effects by negative signaling or simply as a molecular decoy. Attenuating roles of IL-13Rα2 could explain the lack of evidence for IL-13 effects on T cells or an enhancing role could explain the effect of IL-13 effect on airways hyperreactivity and eosinophil survival distinct from IL-4.. Interestingly, we find that the absence of IL-13Rα2 correlates with nearly complete loss of serum IL-13 and an increase in tissue IL-13 in IL-13Rα2−/− mice. The lack of serum IL-13 cannot be explained by a lack of IL-13 production in IL-13Rα2−/− mice as IL-13 is present in tissues of IL-13Rα2−/− and is produced by activated IL-13Rα2−/− immune cells. Serum IL-13Rα2 may act as a ...
TY - JOUR. T1 - Differential capacity of human interleukin-4 and interferon-alpha monocyte-derived dendritic cells for cross-presentation of free versus cell-associated antigen. AU - Ruben, J.M.. AU - Bontkes, H.J.. AU - Westers, T.M.. AU - Hooijberg, E.. AU - Ossenkoppele, G.J.. AU - de Gruijl, T.D.. AU - van de Loosdrecht, A.A.. PY - 2015. Y1 - 2015. U2 - 10.1007/s00262-015-1741-1. DO - 10.1007/s00262-015-1741-1. M3 - Article. C2 - 26216454. VL - 64. SP - 1419. EP - 1427. JO - Cancer Immunology and Immunotherapy. JF - Cancer Immunology and Immunotherapy. SN - 0340-7004. IS - 11. ER - ...
Description: The IL-1 family is comprised of 11 structurally related ligands, including recently re-named IL-36 α (IL-1F6), β (IL-1F8) and γ (IL-1F9). IL-36γ is highly expressed in psoriatic plaques and in tissues containing epithelial cells. IL-36γ signals through the IL-1Rrp2 (IL-1R6) receptor, which is primarily expressed on certain dendritic cells. The interaction of the IL-1Rrp2 receptor with IL-36 ligands induces dendritic cell maturation and activation. IL-36γ also functions as an agonist of NF-kappaB, and can stimulate the inflammatory response in bronchial epithelial cells. Recombinant human IL-36γ is a 17.0 kDa protein containing 152 amino acid residues ...
Not only IFN-γ of Th1 cytokine but also IL-4 or IL-13 of Th2 cytokines enhance IL-12 secretion by CD40L from mature dendritic cells derived from human monocytesNot only IFN-γ of Th1 cytokine but also IL-4 or IL-13 of Th2 cytokines enhance IL-12 secretion by CD40L from mature dendritic cells derived from human monocytes ...
The IL-1 family is comprised of 11 structurally related ligands, including the recently re-named IL-36RA (IL-1F5), IL-36α (IL-1F6), IL-36β
Interleukiin-10 ehk IL-10 on paljude selgroogsete loomade mitmete lümfisüsteemi rakkude poolt teatud sündmuste korral vabastatavad endogeensed lühiajalised valgulised signaalmolekulid, mis vahendavad tõenäoliselt põletikuvastaseid jaimmunosupressiivseid toimeid (immuunvastuse pidurdamine) ning mis liigitatakse põletikuvastaste tsütokiinide hulka. IL-10-t toodavad ja vabastavad T- (CD4 T-rakud, CD8 T-rakud jt), B-rakud, monotsüüdid, dendriitrakud ja makrofaagid, aga ka kesknärvisüsteemi liigitatud gliiarakud jpt. Eri tüüpi tsütokiinid kasutavad nn segasignaale, nii näiteks inhibeerib IL-4 ja IL-10 eritamine Th1- tüüpi immuunvastuste supressiooni, vähendamaks makrofaagidelt tuleva IL-12 tootmist. Põletikutoime arvatakse seisnevat põletikutsütokiinide, nagu IFN-γ, IL-2, IL-3, TNFα ja GM-CSF, sünteesi inhibeerimises. Interleukiin-10 on 2. rühma (IL10- perekonna), mis koosneb interleukiin-10, IL-19, IL-20, IL-22, IL-24 (Mda-7) ja IL-26, interferoonidest (IFN-alfa, -beeta, ...
Overcoming dendritic cell tardiness to triumph over IL-13 receptor: a strategy for the development of effective pediatric vaccines.: Neonatal exposure to antige
Receptors, Interleukin-4, Type I information including symptoms, causes, diseases, symptoms, treatments, and other medical and health issues.
Your trusted lab partner for Interleukin-8 (IL-8) Serum testing, Viracor Eurofins delivers your results faster, when it matters most.
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Retraction: "Role of interleukin 12..." by van Parijs, L et al. Journal of Experimental Medicine published online 13 April 2009 ... Role of interleukin 12 and costimulators in T cell anergy in vivo. Journal of Experimental Medicine 186(7): 1119-28 (6 October ... 2005 [12] Chronicle of Higher Education, "MIT Fires Biology Professor Who Admitted Faking Data," 11 Nov. 2005 [13] (Payment or ... Kelly E, Won A, Refaeli Y, and van Parijs L (2007) Erratum: IL-2 and related cytokines... Journal of Immunology 179(12): 8569 ( ...
Singh, V. K. (1996). "Plasma increase of interleukin-12 and interferon-gamma. Pathological significance in autism". Journal of ...
2004). "Anti-Interleukin-12 Antibody for Active Crohn's Disease". New England Journal of Medicine. 351 (20): 2069-79. doi: ... 18 (11-12): 1113-20. doi:10.1046/j.1365-2036.2003.01793.x. PMID 14653831. S2CID 28389098. Hanauer, Stephen B.; Korelitz, Burton ... 7 (12): CD008870. doi:10.1002/14651858.CD008870.pub2. PMC 6457996. PMID 27372735. Gopal, Latha; Nachimuthu, Senthil (June 16, ... ABT-874 is a human anti-IL-12 monoclonal antibody being developed by Abbott Laboratories in conjunction with Cambridge Antibody ...
Interleukin-1 receptor-associated kinase deficiency is an inherited disorder of the immune system. This immunodeficiency leads ... Interferon-γ (IFN-γ)/interleukin-12 (IL-12) pathway deficiencies belongs to rare innate immune defects. They are characterized ... Dorman SE, Holland SM (2000). "Interferon-gamma and interleukin-12 pathway defects and human disease" (PDF). Cytokine & Growth ... Rosenzweig SD, Holland SM (February 2005). "Defects in the interferon-gamma and interleukin-12 pathways". Immunological Reviews ...
Rosenzweig, Sergio D.; Holland, Steven M. (2005). "Defects in the interferon-γ and interleukin-12 pathways". Immunological ... Ramirez-Alejo N, Santos-Argumedo L (May 2014). "Innate defects of the IL-12/IFN-γ axis in susceptibility to infections by ... 2006-12-01). "Inborn errors of IL-12/23- and IFN-γ-mediated immunity: molecular, cellular, and clinical features". Seminars in ... 2004-12-11). "Clinical features of dominant and recessive interferon γ receptor 1 deficiencies". The Lancet. 364 (9451): 2113- ...
Interferon-gamma and interleukin-12 pathway defects and human disease. Cytokine Growth Factor Rev. 2000;11(4):321-333. Newport ... Inherited disorders of the interleukin-12/23-interferon gamma axis. In: Ochs HD, Smith CIE, Puck JM, e eds. Primary ...
Marchant, A.; Devière, J; Byl, B; De Groot, D; Vincent, JL; Goldman, M. (1994). "Interleukin-10 production during septicaemia ... Goriely, S; Neurath, MF; Goldman, M (2008). "How microorganisms tip the balance between interleukin-12 family members". Nature ... "A defect in nucleosome remodeling prevents IL-12(p35) gene transcription in neonatal dendritic cells". Journal of Experimental ...
Borges W, Augustine N, Hill H (2000). "Defective interleukin-12/interferon-gamma pathway in patients with ... TYK2 may present as HIES,[12] although more often only with immunodeficiency.[13] ... Yang, Linlin; Fliegauf, Manfred; Grimbacher, Bodo (2014-12-01). "Hyper-IgE syndromes: reviewing PGM3 deficiency". Current ...
Krueger G, Langley R, Leonardi C, Yeilding N, Guzzo C, Wang Y, Dooley L, Lebwohl M (2007). "A human interleukin-12/23 ... Retrieved 12 Dec 2014.. *^ a b c d Lindenmann, Jean (1984). "Origin of the Terms 'Antibody' and 'Antigen'". Scand. J. Immunol. ... Retrieved 12 December 2008.. *^ "Emil von Behring - Biography". Archived from the original on 18 November 2010. Retrieved 5 ... 12 (6): 1130-1135. doi:10.1021/bi00730a018. PMID 4569769.. *^ Tomasi TB (1992). "The discovery of secretory IgA and the mucosal ...
DP1 activation blocks the production of interleukin 12 by dendritic cells; this biases the development of naïve T lymphocytes ... 2003). "Prostaglandin D2 inhibits the production of interleukin-12 in murine dendritic cells through multiple signaling ... 55 (12): 1495-1505. doi:10.1007/s40262-016-0409-0. PMC 5107196. PMID 27286723. Norman P (Jan 2014). "Update on the status of ... 57 (4): 307-12. doi:10.2332/allergolint.08-RAI-0033. PMID 18946232. Hohjoh H, Inazumi T, Tsuchiya S, Sugimoto Y (2014). " ...
Purification from the serum demonstrated that the factor was Interleukin 12. During the 12 years that Anderson has been in ... Ther.,12: 193-204, 2001. Anderson, W.F.: Human Gene Therapy. Nature, 392 [SUPP]: 25-30, 1998. Anderson, W. F.: The best of ... A thorough immune status follow-up was done after 12 years: she remained healthy with 20% of her lymphocytes still carrying an ... Persistence and expression of the adenosine deaminase gene for 12 years and immune reaction to gene transfer components: long- ...
Ohteki, T.; Fukao, T.; Suzue, K.; Maki, C.; Ito, M.; Nakamura, M.; Koyasu, S. (1999). "Interleukin-12 dependent interferon-g ... Sugamura, K.; Asao, H.; Kondo, M.; Tanaka, N.; Ishii, N.; Ohbo, K.; Nakamura, M.; Takeshita, T. (1996). "The interleukin-2 ... 1996). "Modulation of hematopoiesis in mice with a truncated mutant of the interleukin-2 receptor gamma chain". Blood. 87 (3): ... 1997). "Lymphohaematopoietic abnormalities and systemic lymphoproliferative disorder in interleukin-2 receptor gamma chain- ...
Bacon CM, Petricoin EF, Ortaldo JR, Rees RC, Larner AC, Johnston JA, O'Shea JJ (August 1995). "Interleukin 12 induces tyrosine ... It is required for the development of Th1 cells from naive CD4+ T cells and IFN-γ production in response to IL-12. Human as ... Two chains of IL-12 receptor form heterodimer after IL-12 binding and activate the receptor associated JAK kinases, termed JAK2 ...
While at the Institute, he discovered the cytokine Interleukin-12 (IL-12). He is the director of the Cancer and Inflammation ...
... mediated delivery of a plasmid coding gene for interleukin-12 (pIL-12) was performed and safety, tolerability ... "Phase I trial of interleukin-12 plasmid electroporation in patients with metastatic melanoma". Journal of Clinical Oncology. 26 ... 58 (1): 1-12. Bibcode:1990BpJ....58....1C. doi:10.1016/S0006-3495(90)82348-1. PMC 1280935. PMID 2383626. Sengel, Jason T.; ... Bibcode:2017NatNa..12..974G. doi:10.1038/nnano.2017.134. ISSN 1748-3395. PMC 5814120. PMID 28785092. McClenaghan NH (May 2007 ...
"Phase I trial of interleukin-12 plasmid electroporation in patients with metastatic melanoma". Journal of Clinical Oncology. 26 ... 37 (12): 2615-25. CiteSeerX doi:10.1007/s10439-009-9796-9. PMID 19757056. S2CID 985854. Edd JF, Horowitz L, ... Xie F, Varghese F, Pakhomov AG, Semenov I, Xiao S, Philpott J, Zemlin C (2015-12-14). "Ablation of Myocardial Tissue With ... 10 (12): e0144833. Bibcode:2015PLoSO..1044833X. doi:10.1371/journal.pone.0144833. PMC 4687652. PMID 26658139. Mandel Y, Laufer ...
Interleukin-2 production by tumor cells bypasses T helper function in the generation of an antitumor response. Cell, 60(3), pp. ... Influence of interleukin 12 on p53 peptide vaccination against established Meth A sarcoma. Proceedings of the National Academy ... Treatment of established renal cancer by tumor cells engineered to secrete interleukin-4. Science, 254(5032), pp.713-716.doi: ... Nature medicine, 1(12), p.1297.doi:10.1038/nm1295-1297 Fearon, E.R., Pardoll, D.M., Itaya, T., Golumbek, P., Levitsky, H.I., ...
Tosato, G; Jones, Kd (March 1990). "Interleukin-1 induces interleukin-6 production in peripheral blood monocytes". Blood. 75 (6 ... Taga, K; Mostowski, H; Tosato, G (June 1993). "Human interleukin-10 can directly inhibit T-cell growth". Blood. 81 (11): 2964- ... Sgadari, C; Angiolillo, Al; Tosato, G (May 1996). "Inhibition of angiogenesis by interleukin-12 is mediated by the interferon- ... 12 August 2014. Retrieved 2 March 2021. This article incorporates text from this source, which is in the public domain. Stalvey ...
"Importance of the MKK6/p38 pathway for interleukin-12-induced STAT4 serine phosphorylation and transcriptional activity". Blood ... MAP2K6 has been shown to interact with TAOK2,[7] ASK1,[8][9] MAPK14[7][10][11][12] and MAP3K7.[13][14][15][16] ...
"Autocrine production of interleukin 6 causes multidrug resistance in breast cancer cells". Cancer Research. 61 (24): 8851-8858 ... 41 (12): 6034-6044. doi:10.1093/nar/gkt305. ISSN 0305-1048. PMC 3695503. PMID 23630318. Xu, W.; Cai, B.; Chen, J.l.; et al. ( ...
These include interleukin 12 (IL-12), interferon gamma (IFNγ) and interleukin 10 (IL-10). Some pharmacological studies such as ... 5 (12): e15963. doi:10.1371/journal.pone.0015963. PMC 3013144. PMID 21209842. [Holmes B (15 January 2011). "Is coffee the real ... 5 (12): e15963. doi:10.1371/journal.pone.0015963. PMC 3013144. PMID 21209842. Maxwell CR, Spangenberg RJ, Hoek JB, Silberstein ... 164 (12): 1334-40. doi:10.1001/archinte.164.12.1334. PMID 15226168. Piasecki TM, Robertson BM, Epler AJ (June 2010). "Hangover ...
Specifically, it blocks interleukin IL-12 and IL-23 which help activate certain T-cells. It binds to the p-40 subunit of both ... Koutruba, N; Emer, J; Lebwohl, M (15 April 2010). "Review of ustekinumab, an interleukin-12 and interleukin-23 inhibitor used ... Manufactured in the Netherlands, the subcutaneous human interleukin 12 and interleukin 23 antagonist are naturally occurring ... 12 Sept 2017 "Janssen's Stelara Gets FDA Approval for Ulcerative Colitis". FDA News. WCG. Retrieved October 31, 2019. "Stelara ...
2003). "Interleukin-23 rather than interleukin-12 is the critical cytokine for autoimmune inflammation of the brain". Nature. ... "Interleukin-23 rather than interleukin-12 is the critical cytokine for autoimmune inflammation of the brain". Nature. 421 (6924 ... 155 (12): 5769-5776. PMID 7499865. Smeyne, RJ; Klein, R; Schnapp, A; Long, LK; Bryant, S; Lewin, A; Lira, SA; Barbacid, M. ( ... 12 (5): 495-503. doi:10.1016/s1074-7613(00)80201-0. PMID 10843382. Vassileva, G; Soto, H; Zlotnik, A; Nakano, H; Kakiuchi, T; ...
The helminths down regulate the pro-inflammatory Th1 cytokines, Interleukin-12 (IL-12), Interferon-Gamma (IFN-γ) and Tumour ... Co-evolution with helminths has shaped some of the genes associated with Interleukin expression and immunological disorders, ... June 2009). "Parasites represent a major selective force for interleukin genes and shape the genetic predisposition to ... "Killing of human Herpes virus 6-infected cells by lymphocytes cultured with interleukin-2 or -12". Pediatrics International. 42 ...
Liu J, Guan X, Tamura T, Ozato K, Ma X (2005). "Synergistic activation of interleukin-12 p35 gene transcription by interferon ... "Ubiquitin-dependent degradation of interferon regulatory factor-8 mediated by Cbl down-regulates interleukin-12 expression". J ... 6 (12): 1841-51. doi:10.1158/1541-7786.MCR-08-0280. PMC 2605678. PMID 19074829. Schaper F, Kirchhoff S, Posern G, Köster M, ...
... interleukin-1 beta (IL-1β), interleukin-6 (IL-6), tumor necrosis factor (TNFα), and macrophage inflammatory protein-2 (MIP-2), ... Cooper, AM; Magram, J; Ferrante, J; Orme, IM (Jul 7, 1997). "Interleukin 12 (IL-12) is crucial to the development of protective ... Trinchieri, G (1995). "Interleukin-12: a proinflammatory cytokine with immunoregulatory functions that bridge innate resistance ... Oswald, IP; Dozois, CM; Petit, JF; Lemaire, G (April 1997). "Interleukin-12 synthesis is a required step in trehalose ...
July 1994). "Structure and mechanism of interleukin-1 beta converting enzyme". Nature. 370 (6487): 270-5. doi:10.1038/370270a0 ... 1993). "The C. elegans cell death gene ced-3 encodes a protein similar to mammalian interleukin-1 beta-converting enzyme". Cell ... Structure of caspase-1 (CASP1), originally called interleukin-1 beta-converting enzyme (ICE), the first human caspase to be ... "Gasdermin D is an executor of pyroptosis and required for interleukin-1β secretion". Cell Research. 25 (12): 1285-1298. doi ...
Identification of a coordinate regulator of interleukins 4, 13, and 5 by cross-species sequence comparisons.. Science. 2000, ... 2006年一篇發表在《自然》的研究報告中[12],研究人員發現在人類與其他哺乳類DNA序列中的拷貝數
Instead, plasma cells are identified through flow cytometry by their additional expression of CD138, CD78, the Interleukin-6 ... ISBN 0-12-053641-2.. *^ Bertil Glader; Greer, John G.; John Foerster; Rodgers, George G.; Paraskevas, Frixos (2008). Wintrobe's ...
Interleukin-10 determines viral clearance or persistence in vivo. Nature Medicine. November 2006, 12 (11): 1301-9. PMC 2535582 ... Modulation of autoimmune diseases by interleukin (IL)-17 producing regulatory T helper (Th17) cells. The Indian Journal of ... 其他几类不表达FOXP3基因的T细胞具有免疫抑制作用,例如Tr1细胞和Th3细胞。Tr1与IL-10相关,Th3与TGF-beta相关。最近,Th17细胞也被列入此类免疫抑制细胞之中[12]。 ... doi:10.1128/JVI.00889-12.. *^ Wherry
EPO " Interleukin " G-CSF " OM " LIF " IFN " CD " TNF-α " LT " Ligand " TGF " MIF ... C5a " CCL2 " CCL5 " GSM-CSF " LPS " MCP-1 " IL-1 " IL-2 " IL-3 " IL-4 " IL-5 " IL-6 " IL-8 " IL-10 " IL-12 " IL-13 " IL-15 " IL ...
... the Antigen-Presenting Cell causes a response in a TH2 lymphocyte which produce the cytokine interleukin-4 (IL-4). The TH2 ... Collingridge GL, Isaac JT, Wang YT (2004). "Receptor trafficking and synaptic plasticity". Nat Rev Neurosci 5(12): 952-962, ... This process may increase the risk for relapse in addicts attempting to quit.[12] ...
CXCL10, hemokin (C-X-C motiv) ligand 10, ili IP-10[1] je mali citokin iz CXC hemokin familije koji je takođe poznat kao 10 kDa interferon-gama-inducirani protein (γ-IP10 ili IP-10). CXCL10 luči nekoliko ćelijski tipova u responsu na IFN-γ. U te ćelijske tipove spadaju monociti, endotelijalne ćelije i fibroblasti.[2] CXCL10 hemokinu je bilo pripisano nekoliko uloga, kao što su hemoatrakcija monocita/makrofaga, T ćelija, NK ćelija, i dendritskih ćelija, promocija adhezije T ćelija na endotelijalne ćelije, antitumorska aktivnost, i inhibicija formiranja kolonija kičmene moždine i angiogeneze.[3][4] CXCL10 gen je lociran na ljudskom hromozomu 4 u klasteru sa nekoliko drugih CXC hemokina.[5] Ovaj hemokin dejstvuje putem vezivanja na CXCR3 hemokin receptore na ćelijskoj površini.[6]. Tri-dimenzionalna kristalna struktura ovog hemokina je bila utvrđena u 3 različite grupe uslova u rezoluciji do 1.92 A.[7] PDB pristupni kodovi za CXCL10 strukture su: 1lv9, 1o7y, 1o7z i 1o80.[8] ...
Stimulation of interleukin-2 production by substance p". Journal of Neuroimmunology. 37 (1-2): 65-74. doi:10.1016/0165-5728(92) ... 12 (6): 909-21. doi:10.2174/138945011795528796. PMID 21226668.. *^ Seckl MJ, Higgins T, Widmer F, Rozengurt E (Jan 1997). "[D- ... 12] The SP receptor promoter contains regions that are sensitive to cAMP, AP-1, AP-4, CEBPB,[13] and epidermal growth factor. ... 19-12-29-71-63(69)70/h4-9,15-18,37,40-49H,10-14,19-36,64-65H2,1-3H3,(H2,66,82)(H2,67,83)(H2,68,85)(H,72,86)(H,73,84)(H,74,89)(H ...
The release of proinflammatory cytokines such as interleukin-1 by activated leukocytes triggered by lipopolysaccharides, ... Maier SF, Wiertelak EP, Martin D, Watkins LR (October 1993). "Interleukin-1 mediates the behavioral hyperalgesia produced by ... 12] This occurs where the pain fibres synapse to pain pathway, the periaqueductal grey. Amplification in the spinal cord may be ...
Interleukins/T-cell growth factors (see here instead). *Leukemia inhibitory factor (LIF) ... 12 July 2013.. *^ a b c d "GIOTRIF® Afatinib (as afatinib dimaleate)" (PDF). TGA eBusiness Services. Boehringer Ingelheim Pty ... doi:10.1016/S1470-2045(12)70087-6. PMID 22452896.. *^ Yang JC, Wu YL, Schuler M, Sebastian M, Popat S, Yamamoto N, et al. ( ... 10-12,14,16H,7-9,13H2,1-2H3,(H,30,32)(H,27,28,29)/b4-3+/t16-/m0/s1 Y ...
Chem. 380 (12): 1443-7. PMID 10661873. doi:10.1515/BC.1999.186. CS1 održavanje: Eksplicitna upotreba et al. (link) ...
... , hemokin (C-X-C motiv) ligand 16, je mali citokin iz CXC hemokin familije. On je veći od drugih hemokina (sadrži 254 aminokiselina). CXCL16 se sastoji od CXC hemokin domaina, mucinu-slične stabljike, transmembranskog domaina i citoplazmatičnog repa koji sadrži potentno mesto tirozin fosforilacije koje može da veže SH2.[1] Ovo su neuobičajene osobine za hemokin, i omobućavaju CXCL16 da bude izražen kao molekul na ćelijskoj površini, kao i rastvorni hemokin.[2] CXCL16 proizvode dendritiske ćelije koje se mogu naći u T ćelijskim zonama limfoidnih organa, i ćelije iz crvene pulpe slezine.[1] Među ćelijama koje se vezuju i migriraju u responsu na CXCL16 su nekoliko podgrupa T ćelija, i NKT ćelije.[1] CXCL16 interaguje sa hemokin receptorom CXCR6, takođe poznatim kao Bonzo.[3][1] Ekspresiju CXCL16 indukuju inflamatorni citokini IFN-gama i TNF-alfa.[2] Gen za ljudski CXCL16 je lociran na hromozomu 17.[1][4] ...
... associations and interaction with the interleukin-1alpha-889 C/T polymorphism". Hum. Immunol. 65 (6): 622-31. doi:10.1016/j. ... 41 (3): 301-12. doi:10.1007/BF00284764. PMID 649158.. *^ Ludwig H, Polymenidis Z, Granditsch G, Wick G (November 1973). "[ ... 12] This association later migrated to the "B8-DRw3" (Current: B8-DR3) region.[13] There are two major DR3 haplotypes in Europe ... 12 (5): 381-6. doi:10.1111/j.1399-0039.1978.tb01347.x. PMID 85353.. ...
Theorell, T.; Hasselhorn, H.; Vingård, E.; Andersson, B. (2000). "Interleukin 6 and cortisol in acute musculoskeletal disorders ... doi:10.1016/S0140-6736(12)60994-5 *^ Huang, Y., Xu, S., Hua, J. et al. (2015). Association between job strain and risk of ... Newsletter of the Society for Occupational Health Psychology, 5, 3-4. [12] ... Journal of Occupational and Environmental Medicine, 55(12 Suppl.), S8-S11. doi:10.1097/JOM.0000000000000037 ...
46 (12): 1930-1931. doi:10.1086/588563. PMID 18540808.. *^ Lahmer, Tobias; da Costa, Clarissa Prazeres; Held, Jürgen; Rasch, ... 12] and various mushrooms, such as lingzhi, shiitake, chaga, and maitake, which are under preliminary research for their ...
"Polymorphisms of the interleukin-1 beta gene are associated with increased risk of non-small cell lung cancer". International ... The Pribnow box has a 6 bp region centered around the -10 position and an 8-12 bp sequence around the -35 region that are both ... 12] Similarly, in humans only 24% of genes have promoter regions containing the TATA box.[13] Genes containing the TATA-box ... 12][14] Generally, TATA-containing genes are not involved in essential cellular functions such as cell growth, DNA replication ...
... interleukin-5)可促進嗜曙紅顆粒白血球成長及分化。[13] ... 嗜曙紅顆粒白血球細胞核同樣分為多葉(2到4葉)。因在血液中有較強脫粒(英語:degranulation)傾向,嗜曙紅顆粒白血球眾的顆粒數目不定。[12]。,嗜曙紅顆粒白血球的顆粒中包含
... and interleukin-1 in modulating progesterone and oestradiol production by human luteinized granulosa cells in culture. Hum. ... 多囊卵巢綜合症目前並無特效藥[5]。治療則包括減重和運動等轉變生活型態的方式[11][10],避孕藥物也許對於調整經期、抑制多餘的毛髮生長和青春痘有所幫助。二甲双胍和抗雄性激素可能有所幫助。另外針對青春痘和多毛等症狀的症狀治療也有一定效果[12
... interleukin-1 (IL-1) - interleukin-2 (IL-2) - interleukin-4 (IL-4) - interleukin-12 (IL-12) - interleukins - International ...
negative regulation of interleukin-6 secretion. • negative regulation of neuron death. • negative regulation of matrix ... 12, no. 2. - P. 117-126. - DOI:10.1093/dnares/12.2.117. - PMID 16303743. ...
... interleukin 7 receptor chain α deficiency, CD45 deficiency, CD3δ/CD3ε deficiency. T-/B- SCID (both T and B cells absent): RAG 1 ... These new methods have reduced culture time to 10-12 days by using specific cytokines from adult donors or virus-naive cord ... Retrieved 12 April 2016. Boyle JM, Buckley RH (2007). "Population prevalence of diagnosed primary immunodeficiency diseases in ... IL-12 and IL-23 β1 chain deficiency IL-12p40 deficiency Interferon γ receptor 1 deficiency Interferon γ receptor 2 deficiency ...
Interleukins/T-cell growth factors (see here instead). *Leukemia inhibitory factor (LIF) ... 12] BDNF is also expressed in the retina, kidneys, prostate, motor neurons and skeletal muscle and it is also found in saliva.[ ... 33 (8): 1508-12. doi:10.1016/j.pnpbp.2009.08.011. PMID 19720106. S2CID 43300334.. ...
An example of an autocrine agent is the cytokine interleukin-1 in monocytes. When interleukin-1 is produced in response to ... Interleukin 6 ACRONYM (IL-6) is a cytokine that is important for many aspects of cellular biology including immune responses, ... 117 (12): 3846-56. doi:10.1172/JCI31871. PMC 2096430 . PMID 18060032. Hartman, Zachary C.; Yang, Xiao-Yi; Glass, Oliver; Lei, ... 12 (5): 445-56. doi:10.1016/j.ccr.2007.08.029. PMC 3431210 . PMID 17996648. Marek, Lindsay; Ware, Kathryn E.; Fritzsche, Alexa ...
यह सामग्री क्रियेटिव कॉमन्स ऍट्रीब्यूशन/शेयर-अलाइक लाइसेंस के तहत उपलब्ध है; अन्य शर्ते लागू हो सकती हैं। विस्तार से जानकारी हेतु देखें उपयोग की शर्तें ...
2003). "Role of the pro-inflammatory cytokines tumor necrosis factor-alpha, interleukin-1 beta, interleukin-6 and interleukin-8 ... Činjenica da je interleukin 8 sekrecija povećana sa oksidant stresom, i obrnuto, interleukin 8 uzrokujući regrutovanje ... Interleukin 8 receptor, beta. Reference[uredi - уреди , uredi izvor]. *↑ Wolff B, Burns AR, Middleton J, Rot A (November 1998 ... vezivanje za interleukin-8 receptor. • vezivanje za proteine. • aktivnost hemokina. Ćelijska komponenta. • ekstracelularni ...
Rattazzi M, Puato M, Faggin E, et al. (2004). C-reactive protein and interleukin-6 in vascular disease: culprits or passive ... Mastorakos G, Ilias I (2007). Interleukin-6: a cytokine and/or a major modulator of the response to somatic stress. Ann. N. Y. ... Nishimoto N (May 2006). Interleukin-6 in rheumatoid arthritis. Curr Opin Rheumatol 18 (3): 277-81. PMID: 16582692. DOI: 10.1097 ... Smith PC, Hobisch A, Lin DL, Culig Z, Keller ET (March 2001). Interleukin-6 and prostate cancer progression. Cytokine Growth ...
4,0 4,1 4,2 4,3 4,4 4,5 4,6 Kolls JK, Lindén A (2004). "Interleukin-17 family members and inflammation". Immunity 21 (4): 467- ... "Interleukin-23 promotes a distinct CD4 T cell activation state characterized by the production of interleukin-17". J. Biol. ... Interleukin 17 (IL-17, ili IL-17A) je osnivački član grupe citokina poznatih kao IL-17 familija. IL-17A, je originalno (1993. ... 17,0 17,1 17,2 17,3 Moseley TA, Haudenschild DR, Rose L, Reddi AH (2003). "Interleukin-17 family and IL-17 receptors". Cytokine ...
Interleukin receptors *IL2R / IL2RA/IL2RB / IL15R. *IL4R / IL13R / IL13RA1 / IL13RA2. *IL7R / IL7RA ... CD30-positive cases of the NK cell lymphoma, extranodal NK/T-cell lymphoma, nasal type[12] ...
Interleukins/T-cell growth factors (see here instead). *Leukemia inhibitory factor (LIF) ... Retrieved 2013-12-08.. *^ a b c d "Highlights of Prescribing Information (Macugen)". Food and Drug Administration: 3-12. July ... Retrieved 2013-12-08.. *^ Arevalo, JF; Fromow-Guerra, Jans; Sanchez, Juan G.; Maia, Mauricio; Berrocal, Maria H.; Wu, Lihteh; ... results of the Pan-American Collaborative Retina Study Group at 12 months follow up". Retina. 28 (10): 1387-94. doi:10.1097/IAE ...
Singh B, Schwartz JA, Sandrock C, Bellemore SM, Nikoopour E (2013). „Modulation of autoimmune diseases by interleukin (IL)-17 ... Sluzokožno povezani invarijantni T limfociti (MAIT) su posebna vrsta T ćelija, čije postojanje je objavljeno 2009.[10][11][12][ ...
interleukin 12 receptor subunit beta 2provided by HGNC. Primary source. HGNC:HGNC:5972 See related. Ensembl:ENSG00000081985 MIM ... interleukin-12 receptor subunit beta-2. Names. IL-12 receptor subunit beta-2. IL-12R subunit beta-2. interleukin 12 receptor, ... IL12RB2 interleukin 12 receptor subunit beta 2 [ Homo sapiens (human) ] Gene ID: 3595, updated on 1-Jun-2020 ... IL12RB2 interleukin 12 receptor subunit beta 2 [Homo sapiens] IL12RB2 interleukin 12 receptor subunit beta 2 [Homo sapiens]. ...
Interleukin-12 receptor beta1 deficiency presenting as recurrent Salmonella infection.. Staretz-Haham O1, Melamed R, Lifshitz M ... We describe a child with interleukin-12 receptor beta1 (IL-12Rbeta1) deficiency caused by a homozygous IL12RB1 large deletion ...
The pneumococcus is a bacterium that frequently causes infections in the lungs, ears, sinus cavities, and meninges. During these infections, body defenses are triggered by tissue-resident cells that use specialized receptors, such as Toll-like receptors (TLRs), to sense the presence of bacteria. We show here that pneumococci are predominantly detected by TLRs that are located inside intracellular vacuoles, including endosomes, where... ...
Whilst IL-23 and IL-12 are pro-inflammatory regulators of Th1 and Th17 responses, IL-27 is bidirectional for inflammation and ... Whilst IL-23 and IL-12 are pro-inflammatory regulators of Th1 and Th17 responses, IL-27 is bidirectional for inflammation and ... but it is becoming apparent that IL-12 cytokine family members including IL-12, IL-23, IL-27, and IL-35 are also involved. ... but it is becoming apparent that IL-12 cytokine family members including IL-12, IL-23, IL-27 and IL-35 are also involved. ...
Pharmacological Actions : Anti-Inflammatory Agents, Immunomodulatory, Interleukin-10 upregulation, Interleukin-12 upregulation ... Pharmacological Actions : Immunomodulatory, Interleukin-10 downregulation, Interleukin-12 upregulation, Interleukin-4 ... Pharmacological Actions : Cyclooxygenase 2 Inhibitors, Immunomodulatory, Interleukin-10 downregulation, Interleukin-12 ... 13 Abstracts with Interleukin-12 upregulation Research. Filter by Study Type. Animal Study. ...
Wang KS, Frank DA, Ritz J (May 2000). "Interleukin-2 enhances the response of natural killer cells to interleukin-12 through up ... Interleukin 12 (IL-12) is an interleukin that is naturally produced by dendritic cells, macrophages, neutrophils, and human B- ... Interleukin 12 (IL-12) is produced by activated antigen-presenting cells (dendritic cells, macrophages). It promotes the ... Newport MJ, Holland SM, Levin M, Casanova JL (2007). "Inherited disorders of the interleukin-12/23-interferon gamma axis". In ...
Interleukin-12 Human. 200 [kp_C] in 1 mL. Interferon Gamma-1a (UNII: VC9M78242P) (Interferon Gamma-1a - UNII:VC9M78242P) ... Interleukin-12 Human (UNII: 7B590791ER) (Interleukin-12 Human - UNII:7B590791ER) ... interleukin-12 human, interferon gamma-1a spray. To receive this label RSS feed. Copy the URL below and paste it into your RSS ... interleukin-12 human, interferon gamma-1a spray. Out of scope - Out of scope for RxNorm and will not receive RxNorm normal ...
Differentiation of naïve CD4+ T cells into the T helper type 1 (TH1) phenotype requires signaling by interleukin (IL)-12 ... T helper type 2 cell differentiation occurs in the presence of interleukin-12 receptor β2 chain expression and signaling. J. ... IL-12 treatment of TH2 cells expressing functional IL-12 receptors did not induce the conversion of these cells into the TH1 ... When naïve CD4+ T cells are treated simultaneously with both IL-4 and IL-12, the IL-4 effects predominate and TH2 cells develop ...
Interleukin 12 receptor is a type I cytokine receptor, binding interleukin 12. It consists of beta 1 and beta 2 subunits. Chua ... Receptors,+Interleukin-12 at the US National Library of Medicine Medical Subject Headings (MeSH) v t e. ... Presky DH, Yang H, Minetti LJ, Chua AO, Nabavi N, Wu CY, Gately MK, Gubler U (1996). "A functional interleukin 12 receptor ... "Expression cloning of a human IL-12 receptor component. A new member of the cytokine receptor superfamily with strong homology ...
Interleukin 12 receptor is a type I cytokine receptor, binding interleukin 12. It consists of beta 1[1] and beta 2 subunits.[2] ... Receptors,+Interleukin-12 at the US National Library of Medicine Medical Subject Headings (MeSH) ... "A functional interleukin 12 receptor complex is composed of two beta-type cytokine receptor subunits". Proc. Natl. Acad. Sci. U ... Retrieved from "https://www.wikidoc.org/index.php?title=Interleukin-12_receptor&oldid=1410556" ...
Interleukin-12 in Treating Patients With Advanced Cancer. The safety and scientific validity of this study is the ... Phase I trial of twice-weekly intravenous interleukin 12 in patients with metastatic renal cell cancer or malignant melanoma: ... Agranulocytosis and hemolytic anemia in patients with renal cell cancer treated with interleukin-12. J Immunother. 2001 Jan-Feb ...
... Mazzolini G, Prieto J, Melero I.. Division of Hepatology and Gene Therapy, ... Morning (9:00 - 12:00) *. Midday (12:00 - 16:00) *. Afternoon (16:00 - 21:00) *. * Operating hours based on Spanish (Peninsula ... IL-12 has demonstrated remarkable antitumor activity when used directly as a recombinant protein or when different viral or non ... In addition, IL-12 inhibits tumor angiogenesis mainly through IFN gamma-dependent production of the chemokine IP10. As a result ...
Interleukin-12 subunit betaAdd BLAST. 308. Amino acid modifications. Feature key. Position(s). DescriptionActions. Graphical ... Associates with IL23A to form the IL-23 interleukin, a heterodimeric cytokine which functions in innate and adaptive immunity. ... The heterodimer is known as interleukin IL-12. Heterodimer with IL23A; disulfide-linked. The heterodimer is known as ... sp,Q865W9,IL12B_LAMGL Interleukin-12 subunit beta OS=Lama glama OX=9844 GN=IL12B PE=2 SV=1 ...
R-HSA-6783783 Interleukin-10 signaling. R-HSA-6785807 Interleukin-4 and Interleukin-13 signaling. R-HSA-8984722 Interleukin-35 ... R-HSA-6783783 Interleukin-10 signaling. R-HSA-6785807 Interleukin-4 and Interleukin-13 signaling. R-HSA-8984722 Interleukin-35 ... interleukin-35-mediated signaling pathway Source: Reactome. *negative regulation of interleukin-17 production Source: BHF-UCL ... Interleukin-12 subunit alphaAdd BLAST. 197. Amino acid modifications. Feature key. Position(s). DescriptionActions. Graphical ...
Polyclonal Antibody Immunoprecipitation interleukin-10 Production * Polyclonal Antibody Immunoprecipitation interleukin-1 Beta ... Also showing Polyclonal Antibody Immunoprecipitation Positive Regulation of interleukin-12 Production. Polyclonal Antibody - ... Polyclonal Antibody Immunoprecipitation interleukin-1 Beta Production * Polyclonal Antibody Immunoprecipitation Cytokine ... members of the IL-1 receptor family share a conserved stretch of approximately 200 amino acids known as the Toll/Interleukin-1 ...
6. Interleukin-12 (IL-12) Inhibitor Pipeline Products in Non-clinical Stages 6.1 Drug Name : Company Name*Product Description ... 5. Interleukin-12 (IL-12) Inhibitor Pipeline Products in Clinical Stages. 5.1 Drug Name : Company Name*Product Description ... 2. Interleukin-12 (IL-12) Inhibitor - Overview. 3. Pipeline Therapeutics*An Overview of Pipeline Products for Interleukin-12 ( ... Table 3: Interleukin-12 (IL-12) Inhibitor Therapeutic Products in Non-clinical Stages. Table 4: Pipeline Assessment by Route of ...
... Vaccine. 2004 ... IL-12 and IFN-gamma productions were dose-dependent on antigens in PS-liposomes. The expression of CD40L on 42-6A cells was ... IL-12 production, but not IFN-gamma, was completely inhibited by the addition of anti-CD40L mAb. These results indicate that PS ... cells in the presence of PS-liposomes encapsulating OVA resulted in high levels of IL-12 and IFN-gamma productions compared ...
Methods: The isolated lymphocytes were cultured in the presence of interleukins (IL)-2, IL-4, and IL-12. Enzyme-linked ... Keywords: HCC, immunotherapy, fine-needle aspirates, tumor infiltrating lymphocytes, interferon-gamma, interleukins ... Conclusion: Our data suggested that IL-12 together with IL-2 caused a suppression of CD4+CD25+ regulatory T-cells and an ... Potential immunotherapeutic role of interleukin-2 and interleukin-12 combination in patients with hepatocellular carcinoma ...
Interleukin 12 (IL-12) is a heterodimeric cytokine with many functions including induction of interferon (IFN)-γ by natural ... Interferon (IFN)-α/β-mediated negative regulation of interleukin 12 (IL-12) and IFN-γ proteins is reported here. Both IFN-α and ... Interferon-α/β inhibition of interleukin 12 and interferon-γ production in vitro and endogenously during viral infection. ... Interferon-α/β inhibition of interleukin 12 and interferon-γ production in vitro and endogenously during viral infection ...
Cua DJ, Sherlock J, Chen Y, Murphy CA, Joyce B, Seymour B et al. Interleukin-23 rather than interleukin-12 is the critical ... Interleukins 27 and 6 induce STAT3-mediated T cell production of interleukin 10. Nat Immunol 2007;8:1363-1371. *CrossRef, ... A dominant function for interleukin 27 in generating interleukin 10-producing anti-inflammatory T cells. Nat Immunol 2007;8: ... Rosas LE, Satoskar AA, Roth KM, Keiser TL, Barbi J, Hunter C et al. Interleukin-27R (WSX-1/T-cell cytokine receptor) gene- ...
Interleukin-12 in Treating Patients With AIDS-Related Kaposis Sarcoma. The safety and scientific validity of this study is the ... RATIONALE: Interleukin-12 may kill tumor cells by stopping blood flow to the tumor and by stimulating a persons white blood ... Patients receive interleukin-12 (IL-12) subcutaneously twice a week (at least 3 days apart) for 6 months. Patients with stable ... Drug Information available for: Interleukin-12 Genetic and Rare Diseases Information Center resources: Soft Tissue Sarcoma ...
Inherited interleukin 12 deficiency in a child with bacille Calmette-Guérin and Salmonella enteritidis disseminated infection. ... Interferon-gamma receptor ligand-binding chain (IFN-gammaR1) or signaling chain (IFN-gammaR2) deficiency, like interleukin 12 ... Mutations in the genes for IFN-gammaR1, IFN-gammaR2, IL-12Rbeta1, and other molecules implicated in IL-12- or IFN-gamma- ... A large homozygous deletion within the IL-12 p40 subunit gene was found, precluding expression of functional IL-12 p70 cytokine ...
Interleukin 27 limits autoimmune encephalomyelitis by suppressing the development of interleukin 17-producing T cells. Nat ... Interleukin-12: Biological Properties and Clinical Application. Michele Del Vecchio, Emilio Bajetta, Stefania Canova, Michael T ... Interleukin-12: Biological Properties and Clinical Application. Michele Del Vecchio, Emilio Bajetta, Stefania Canova, Michael T ... Interleukin-12: Biological Properties and Clinical Application. Michele Del Vecchio, Emilio Bajetta, Stefania Canova, Michael T ...
Plasmid DNA encoding human interleukin 12 (IL-12) was produced under GMP conditions and injected into lesions of nine patients ... Intratumoral injection of DNA encoding human interleukin 12 into patients with metastatic melanoma: clinical efficacy Hum Gene ... Plasmid DNA encoding human interleukin 12 (IL-12) was produced under GMP conditions and injected into lesions of nine patients ... Biopsies of responders showed some increase in IL-12, IP-10, and IFN-(). Serum levels revealed fluctuations. The results show ...
What is interleukin-12 subunit beta? Meaning of interleukin-12 subunit beta medical term. What does interleukin-12 subunit beta ... Looking for online definition of interleukin-12 subunit beta in the Medical Dictionary? interleukin-12 subunit beta explanation ... redirected from interleukin-12 subunit beta) IL12B. A gene on chromosome 5q31.1-q33.1 that encodes interleukin-12B, a cytokine ... It associates with IL23A to form the IL-23 interleukin, a heterodimeric cytokine which functions in innate and adaptive ...
Antibodies for proteins involved in negative regulation of interleukin-12 production pathways, according to their Panther/Gene ... Antibodies for proteins involved in negative regulation of interleukin-12 production pathways; according to their Panther/Gene ...
Interleukin 12 and tumor necrosis factor alpha are costimulators of interferon gamma production by natural killer cells in ... Interleukin 12 and tumor necrosis factor alpha are costimulators of interferon gamma production by natural killer cells in ... Interleukin 12 and tumor necrosis factor alpha are costimulators of interferon gamma production by natural killer cells in ... Interleukin 12 and tumor necrosis factor alpha are costimulators of interferon gamma production by natural killer cells in ...
Phase I trial to study the effectiveness of interleukin-12, paclitaxel, and trastuzumab in treating patients who have solid ... No prior interleukin-12. - No prior trastuzumab (Herceptin®). - At least 3 weeks since prior chemotherapy. - At least 3 weeks ... Phase I Trial of Interleukin-12 in Combination With Paclitaxel Plus Herceptin in Patients With Her2-positive Malignancies. ... OUTLINE: This is a dose-escalation study of interleukin-12.. Patients receive trastuzumab (Herceptin®) IV over 30-90 minutes on ...
Targeting Cytokines of the Interleukin-12 Family in Autoimmunity. Author(s): Bok Yun Kang, Tae Sung Kim. School of Life ... Interleukin (IL)-12 and the more recently discovered IL-23 and IL-27 constitute a unique family of structurally-related, ... Interleukin (IL)-12 and the more recently discovered IL-23 and IL-27 constitute a unique family of structurally-related, ... Therefore, pharmacologically targeting cytokines of the IL-12 family would be useful in the modulation of several autoimmune ...
Pembrolizumab and Recombinant Interleukin-12 in Treating Patients With Solid Tumors ... Pembrolizumab and Recombinant Interleukin-12 in Treating Patients With Solid Tumors Brief description of study. This phase I ... Recombinant interleukin-12 may kill tumor cells by blocking blood flow to the tumor and by stimulating white blood cells to ... Patients receive recombinant interleukin-12 subcutaneously (SC) on days 2, 5, 9, and 12 and pembrolizumab intravenously (IV) ...
  • A child with Bacillus Calmette-Guérin and Salmonella enteritidis infection was found to have a large homozygous deletion within the IL-12 p40 subunit gene, precluding expression of functional IL-12 p70 cytokine by activated dendritic cells and phagocytes. (wikipedia.org)
  • Interleukin 12 receptor is a type I cytokine receptor, binding interleukin 12. (wikipedia.org)
  • Many T-cell-associated cytokines have been implicated in the immunopathogenesis of sarcoidosis, but it is becoming apparent that IL-12 cytokine family members including IL-12, IL-23, IL-27, and IL-35 are also involved. (frontiersin.org)
  • Associates with IL23A to form the IL-23 interleukin, a heterodimeric cytokine which functions in innate and adaptive immunity. (uniprot.org)
  • These results demonstrate a new pathway for regulating cytokine responses, and suggest a mechanism for inhibition of IL-12-dependent immune responses during viral infections. (pnas.org)
  • Interleukin 12 (IL-12) is a heterodimeric cytokine with many functions including induction of interferon (IFN)-γ by natural killer (NK) cells and generation of T helper 1 (Th1) cells producing IFN-γ ( 1 ). (pnas.org)
  • They demonstrate that IFN-α/β inhibition acts on expression in response to known exogenous inducers of IL-12 and is an endogenous component shaping cytokine expression during viral infections. (pnas.org)
  • A large homozygous deletion within the IL-12 p40 subunit gene was found, precluding expression of functional IL-12 p70 cytokine by activated dendritic cells and phagocytes. (jci.org)
  • The immunomodulating and antiangiogenic functions of IL-12 have provided the rationale for exploiting this cytokine as an anticancer agent. (aacrjournals.org)
  • More effective application of this cytokine, and of newly identified IL-12 family members (IL-23 and IL-27), should be evaluated as therapeutic agents with considerable potential in cancer patients. (aacrjournals.org)
  • 12 ) discovered that IL-12, produced by macrophages in response to microbial pathogens, was a key cytokine in TH1 T cell differentiation. (aacrjournals.org)
  • The general model on the biological role of IL-12 predicts that this cytokine is required for resistance to bacterial and intracellular parasites, as well as for the establishment of organ-specific autoimmunity ( 11 ). (aacrjournals.org)
  • This review summarizes the recent findings concerning IL-12 family cytokine-mediated autoreactive inflammatory responses, and also describes some possible therapeutic interventions, including medicinal compounds at mitigating autoimmune inflammation. (eurekaselect.com)
  • We chose to genetically engineer tumor antigen-specific CD8 + T cells to secrete interleukin (IL)-12, a potent proinflammatory and antiangiogenic cytokine capable of activating multiple aspects of innate and adaptive antitumor immunity ( 11 - 20 ). (aacrjournals.org)
  • IL-12 is a potent, well-characterized proinflammatory cytokine. (ascopost.com)
  • Thus, phagocytosis by mechanisms not specific for M. tuberculosis was a sufficient trigger for IL-12 secretion, emphasizing the central role of this cytokine in the initiation of anti-infective immunity. (asm.org)
  • We compared interleukin-12 (IL-12) and other cytokine activities during and after an acute clinical episode in a matched-pair case-control study of young African children who presented with either mild or severe Plasmodium falciparum malaria. (asm.org)
  • Interleukin-12 (IL-12) is a central, regulatory cytokine in cell-mediated immunity. (clinicalconnection.com)
  • Interleukin-12 (IL-12) is a cytokine that exerts a number of regulatory effects on T lymphocytes and natural killer (NK) cells. (bloodjournal.org)
  • By virtue of its effects on CD4 T cells, CD8 T cells, and NK cells, IL-12 seems to be a key cytokine that regulates cell-mediated immune responses. (aacrjournals.org)
  • In several comparative studies, IL 5 -12 was the most effective cytokine gene that could induce eradication of experimental tumors, prevent the development of metastases, and elicit long-term antitumor immunity ( 2 , 3 ). (aacrjournals.org)
  • Local and efficient expression of IL-12 and other cytokine genes in tumors represents an alternative immunotherapeutic approach that may avoid systemic toxicity of recombinant cytokines ( 6 , 26 , 27 ). (aacrjournals.org)
  • Adenoviral recombinant vectors can infect nondividing cells, thus improving transfection efficiency, and can transduce in vivo cytokine genes, including IL-12, into tumors in animal models ( 12 , 33 - 35 ). (aacrjournals.org)
  • IL-12 is a heterodimeric cytokine composed of two covalently linked subunits, p35 and p40, encoded by separate genes ( 12 ). (asnjournals.org)
  • Interleukin 12 (IL-12) is an immunostimulatory cytokine with proven antitumour effect in animal models. (bmj.com)
  • IL-12 is an immunostimulatory cytokine with antitumour effects in animal models of colorectal cancer. (bmj.com)
  • Interleukin-12 (IL-12) is a cytokine well known for its role in immunity. (biomedcentral.com)
  • We show herein that carefully designed protocols, in mice, utilizing IL-12 as an adjuvant to radiation or chemotherapy yield facile and consistent, multilineage hematopoietic recovery from cancer therapy-induced cytopenias, as compared to vehicle and the clinically-utilized cytokine granulocyte colony-stimulating factor (G-CSF) (positive control), while still providing concomitant antitumor responses over and above the effects of the primary therapy alone. (biomedcentral.com)
  • The ability to detect ruminant IL-12 by three methods: ELISA, bioassay with neutralising mAb and cytoplasmic staining, will permit studies of the role of this important cytokine in the immunology and pathogenesis of animal diseases. (ox.ac.uk)
  • These results strongly suggest that LPS induces the release of IL-12, that IL-12 induces the production of IFN-gamma, and that IFN-gamma is the cytokine that primes macrophages and other cell types. (rupress.org)
  • Garlisi, CG & Mastro, AM 1992, ' Characterization of the inhibition of interleukin 2 mRNA accumulation by 12-O-tetradecanoylphorbol-13-acetate in primary lymphocytes ', Lymphokine and Cytokine Research , vol. 11, no. 1, pp. 1-8. (elsevier.com)
  • Interleukin 12 (IL-12) is heterodimeric cytokine produced by dendritic cells, macrophages and neutrophils. (reactome.org)
  • The IL12 receptor is a heterodimer formed by Interleukin-12 receptor subunit beta-1 (IL12RB1) and Interleukin-12 receptor subunit beta-2 (IL12RB2), both of which have extensive homology to IL6ST (gp130), the signal transducing receptor subunit of the IL6-like cytokine superfamily. (reactome.org)
  • As expected, CPP restricted the immune response of mice, whereas oral administration of nLp-nF1 significantly increased total IgG in serum, and cytokine production [interleukin-12 (IL-12) and tumor necrosis factor alpha (TNF-α)] in bone marrow cells. (readbyqxmd.com)
  • IL-12 is a heterodimeric cytokine produced by phagocytic and other cells with important physiologic and pathologic properties. (elsevier.com)
  • To further understand the processes regulating IL-12 production, we determined whether IL-11 regulated monocyte/macrophage production of this cytokine moiety. (elsevier.com)
  • It binds to the INTERLEUKIN-12 SUBUNIT P40 via a disulfide bond that results in the active cytokine. (ctdbase.org)
  • Interleukin (IL)-12, being a major cytokine that induces T helper (Th) 1 differentiation and inflammatory response, has been postulated to be an important mediator of synovial inflammation in rheumatoid arthritis (RA). (biomedcentral.com)
  • IL-12 has been suggested to be the predominant cytokine inducing Th1-like phenotype of CD4 + T cells in rheumatoid arthritis (RA) [ 15 - 17 ], although the evidence is rather indirect. (biomedcentral.com)
  • IL-12 family is unique in comprising the only heterodimeric cytokines, which includes IL-12, IL-23, IL-27 and IL-35. (wikipedia.org)
  • IL-12Rβ2 is considered to play a key role in IL-12 function, since it is found on activated T cells and is stimulated by cytokines that promote Th1 cells development and inhibited by those that promote Th2 cells development. (wikipedia.org)
  • I am working with differents cytokines using a mouse experimental model, and I am wondering if anyone knows of a Company which carries mouse Interleukin 12. (bio.net)
  • This review will discuss the current understanding of etiology and immunopathogenesis of sarcoidosis with a specific focus on the bidirectional impact of IL-12 family cytokines on the pathogenesis of sarcoidosis. (frontiersin.org)
  • Thus the purpose of this review is to discuss the current understanding of the etiology and pathogenesis of sarcoidosis with a focus on a possible role for the IL-12 family cytokines. (frontiersin.org)
  • Interleukin (IL)-12 and the more recently discovered IL-23 and IL-27 constitute a unique family of structurally-related, heterodimeric cytokines, which regulate cell-mediated immune responses and Th1-type inflammatory reactions. (eurekaselect.com)
  • Therefore, pharmacologically targeting cytokines of the IL-12 family would be useful in the modulation of several autoimmune diseases. (eurekaselect.com)
  • IL-23 and IL-12 are heterodimeric cytokines which share the p40 subunit. (biovendor.com)
  • Induction of cytokines, such as IFN-γ ( 4 , 15 - 18 ) and IFN-inducible protein-10 ( 19 ), has also been implicated as a mechanism of antitumor activity of IL-12. (aacrjournals.org)
  • Interleukin (IL)-12 and IL-23 both share the p40 subunit and are key cytokines in the pathogenesis of Crohn's disease. (ebscohost.com)
  • New targets for biologics include cytokines involved in T-cell activation, with antibodies directed against IL-12 and. (ebscohost.com)
  • The cytokines interleukin (IL)-12 and interleukin (IL)-23 have been implicated variously in the pathogenesis of psoriasis and psoriatic arthritis (PsA). (gla.ac.uk)
  • Attenuation of the primary Th1 immune response by immunological inhibition or genetic deletion of Th1 cytokines interferon-γ (IFN-γ) ( 4 , 7 ) and interleukin-12 (IL-12) ( 8 ) or by administration of Th2 cytokines IL-4 and IL-10 ( 9 ) attenuates crescentic GN. (asnjournals.org)
  • Incubation with IL-2 (100 U/ml) or IL-12 (1 ng/ml) for 18 h increased the NK cell activity (using K562 target cells) of both cord blood and adult MNCs, and the combination of IL-2 and IL-12 increased cord blood cytotoxicity threefold, making the cytotoxicity of cord blood cells equivalent to that of adult cells treated with the same cytokines. (asm.org)
  • Previous studies have demonstrated that MNCs from newborn infants have a reduced capacity to produce cytokines, particularly interleukin-2 (IL-2) and gamma interferon (IFN-γ) ( 1 , 6 , 22 , 27 , 48 ), both of which are important in upregulating the cytotoxicity of NK cells ( 4 , 5 , 45 ). (asm.org)
  • CONCLUSIONS: Induction of pro-inflammatory cytokines and associated fluctuations in leukocyte subset counts decrease on repeated administrations of rHuIL-12. (eur.nl)
  • We investigated the phenotypic changes of CB T cells induced by two immunoregulary cytokines, interleukin (IL)-12 and IL-15, alone or in combination. (eurekamag.com)
  • Evaluation of production of cytokines especially IL-10 and IL-12 are important factor in investigation of immune response against a variety of intracellular pathogen, vaccination and other immune reactions. (sid.ir)
  • In the present study, the cytokines induced during this infection determined the level of IL-10 and IL-12 in serum of cattle owners with brucellosis and without brucellosis compared with health controls. (sid.ir)
  • Then 5ee blood sample have been taken from donors and cytokines IL-10 and IL-12 of ELISA were determined by Disclose Company. (sid.ir)
  • The apparent opposing relationship that exists between the two interleukins has led to several studies successfully employing the ratio of these cytokines (usually IL-10/IL-12) in the assessment of immune status, e.g., as a measure of the balance between an anti-inflammatory and a proinflammatory state ( 2 , 3 , 6 ), or Th1 versus Th2 dominance ( 1 ). (asm.org)
  • To investigate the levels of the proinflammatory cytokines tumor necrosis factor  (TNF-), interleukin 23 (IL-23) and IL-17 in patients with psoriasis and psoriatic arthritis with concomitant metabolic syndrome. (termedia.pl)
  • Interleukin 12, interferon gamma, and tumor necrosis factor alpha are the key cytokines of the generalized Shwartzman reaction. (rupress.org)
  • They also demonstrate that this inhibition is not the result of a generalized suppression of macrophage effector function and that the ability to inhibit monocyte/macrophage IL-12 production is not a generalized property of all IL-6-type cytokines. (elsevier.com)
  • ß-glucan may increase serum levels of IL-12, shorten the duration of mechanical ventilation, and reduce organ failure in critically ill multiple-trauma patients. (greenmedinfo.com)
  • Coculture of mouse splenic adherent cells with ovalbumin (OVA)-specific Th1 clone (42-6A) cells in the presence of PS-liposomes encapsulating OVA resulted in high levels of IL-12 and IFN-gamma productions compared with those of positively charged or neutral liposomes. (nih.gov)
  • Although early IL-12 protein expression is induced during murine cytomegalovirus (MCMV) infection ( 12 ) and results in NK cell IFN-γ production ( 12 - 14 ), detectable levels of IL-12 and NK cell IFN-γ production are not observed during LCMV infection ( 12 ). (pnas.org)
  • Due to systemic dosing restrictions, the ability to reach therapeutic levels of IL-12 at the tumor site likely represents a major barrier for treatment of solid tumors. (aacrjournals.org)
  • We show that T cells engineered to produce IL-12 infiltrate tumors in higher numbers than nontransduced cells and continue to secrete supraphysiologic levels of IL-12 directly at the tumor site just before regression. (aacrjournals.org)
  • In vitro , renal tubular epithelial cells produce IL-12 mRNA and low levels of IL-12 protein ( 18 ), and mesangial cells produce IL-12 mRNA and protein after lipopolysaccharide or tumor necrosis factor-α stimulation ( 19 ). (asnjournals.org)
  • Results Intrahepatic injection of HC-Ad/RUmIL-12 and tailored induction regimens allowed the maintenance of safe and efficient levels of IL-12 in vivo. (bmj.com)
  • Interleukin 12 (IL-12) is an interleukin that is naturally produced by dendritic cells, macrophages, neutrophils, and human B-lymphoblastoid cells (NC-37) in response to antigenic stimulation. (wikipedia.org)
  • Interleukin 12 (IL-12) is produced by activated antigen-presenting cells (dendritic cells, macrophages). (wikipedia.org)
  • Tumor cells, dendritic cells, or autologous fibroblasts have been transfected with recombinant adenoviruses or retroviruses to secrete IL-12 locally, showing good efficacy and safety profiles. (cun.es)
  • These results indicate that PS-liposomes encapsulating antigens could enhance antigen-dependent IL-12 production through the interaction between CD40 on macrophage/dendritic cells and CD40L on 42-6A cells. (nih.gov)
  • According to such a model, IL-12, produced by activated hematopoietic phagocytic cells (monocytes, macrophages, and neutrophils), by dendritic cells (DC), and by the recently identified IFN-producing killer DC lineage ( 13 ), acts as a critical regulator of cell-mediated responses. (aacrjournals.org)
  • Migratory CD103+ dendritic cells suppress helminth-driven type 2 immunity through constitutive expression of IL-12. (umassmed.edu)
  • Several studies have engineered tumor cells, dendritic cells, and fibroblasts to overproduce a single-chain functional IL-12, but achieving high local concentrations through a systemic approach remains elusive ( 26 , 30 - 34 ). (aacrjournals.org)
  • Stimulation of the antitumor immune response by dendritic cells (DC) is critically dependent on their tightly regulated ability to produce interleukin-12 (IL-12). (cun.es)
  • This highlights the importance of regulating IL-12 and IL-23 production and we sought to investigate the ability of the mRNA-destabilizing protein, Tristetraprolin (TTP) in regulating IL-12 and IL-23 production by bone-marrow derived dendritic cells (BMDCs). (nus.edu.sg)
  • Using this method native bovine IL-12 was detected in supernatants of dendritic cells (DC) cultured in vitro with a synthetic lipopeptide known to stimulate secretion of IL-12 by human DC. (ox.ac.uk)
  • By promoting IFN-γ production, proliferation, and cytolytic activity of natural killer and T cells, IL-12 induces cellular immunity. (aacrjournals.org)
  • In addition, IL-12 induces an antiangiogenic program mediated by IFN-γ-inducible genes and by lymphocyte-endothelial cell cross-talk. (aacrjournals.org)
  • Administration of recombinant interleukin 12 (IL-12) induces tumor regression that is associated with T-cell infiltration in the OV-HM ovarian carcinoma and CSA1M fibrosarcoma models. (aacrjournals.org)
  • IL-12 enhances the cytolytic activity of cytotoxic T and NK cells, and induces interferon-gamma (IFN gamma) production from T and NK cells. (clinicalconnection.com)
  • Interleukin-12 (IL-12) facilitates cytolytic T-cell responses, enhances the lytic activity of natural killer (NK) cells, and induces the secretion of interferon γ (IFN-γ) by both T and NK cells. (bloodjournal.org)
  • In addition to its immunostimulatory properties, IL-12 induces antiangiogenic effects, thus inhibiting tumor formation and metastases ( 9 , 20 ). (aacrjournals.org)
  • pIL-12 EP monotherapy induces objective tumor responses in a significant proportion of patients (BORR 31%) and treatment was well tolerated. (springer.com)
  • Interleukin-10 (IL-10) induces an immunosuppressive microenvironment including M2 macrophages, inhibiting anti-tumor immunity. (readbyqxmd.com)
  • T cells that produce IL-12 have a coreceptor, CD30, which is associated with IL-12 activity. (wikipedia.org)
  • In vitro studies demonstrated that splenic leukocytes isolated from LCMV-infected mice were primed to produce IL-12 in response to stimulation with Staphylococcus aureus Cowan strain, but that this responsiveness was sensitive to added IFN-α. (pnas.org)
  • Interestingly, HIV infections are also associated with protective CD8 + CTL ( 6 - 9 ), and cells from HIV-infected individuals are inhibited in their ability to produce IL-12 ( 10 , 11 ). (pnas.org)
  • Several preclinical and clinical studies have shown successful tumor regression by direct injection of IL-12 into lesions or genetic manipulations to induce tumor cells to produce IL-12 in situ ( 27 - 30 ). (aacrjournals.org)
  • Although a human B cell line has been shown to produce IL-12, fresh B cells are apparently not producers of IL-12. (acris-antibodies.com)
  • Macrophages and monocytes produce IL-12 as an early response to antigenic stimuli ( 13 ). (asnjournals.org)
  • Antigen-presenting cells produce IL-12 after engagement with activated T cells and co-stimulation via CD40 ligand ( 14 ). (asnjournals.org)
  • These data, together with previous finding that NO synthase inhibitors can ameliorate autoimmune disease in MRL/lpr mice, suggest that high capacity of such mice to produce IL-12 and their greater responsiveness to IL-12, leading to the production of high concentrations of NO, are important factors in this spontaneous model of autoimmune disease. (gla.ac.uk)
  • Because of its ability to induce immune responses and its anti-angiogenic activity, there has been an interest in testing IL-12 as a possible anti-cancer drug. (wikipedia.org)
  • At enhancing tumor immunity, IL-12 acts as a bridge between innate and adaptive immune responses due to its ability to induce proliferation and activation of NK, NKT, and T cells. (cun.es)
  • The role of IL-12 in promoting endogenous protective immune responses to viral infections, however, is only beginning to be understood ( 2 , 3 ). (pnas.org)
  • Our studies of IL-12 administration during lymphocytic choriomeningitis virus (LCMV) infections of mice have demonstrated that high concentrations of the factor are detrimental to expansion of protective CD8 + cytotoxic T lymphocytes (CTL) and synergize with endogenous immune responses to promote systemic toxicities ( 4 , 5 ). (pnas.org)
  • This finding established the central role of IL-12 in a pathway in which innate immune cells drove the adaptive immune response, polarizing naïve CD4 + cells towards the TH1 phenotype. (aacrjournals.org)
  • "Intratumoral delivery of IL-12 stimulated a safe but powerful systemic immune response," Dr. Algazi reported. (ascopost.com)
  • However, metronomic cyclophosphamide plus IL-12 gene therapy failed to increase the number of tumor-infiltrating T lymphocytes and, more importantly, to induce a specific antitumor immune response. (sigmaaldrich.com)
  • Therefore, the hypothesis was considered that combining IL-12 with rituximab would augment the immune-mediated cell lysis induced by rituximab. (bloodjournal.org)
  • 1990). IL-12 also plays an important role in immunomodulation by promoting cell mediated immunity through induction of a class 1 T helper cell (Th1) immune response. (reactome.org)
  • IL‑12 and interferon‑γ serum levels were measured and immunization with LL‑E7P‑IL‑12D was shown to induce an E7‑specific immune response and to confer protection against TC‑1‑induced tumors in vivo. (spandidos-publications.com)
  • Background and aims: Interleukin-12 (IL-12), a p35/p40 heterodimer, plays a pivotal rote in the immune response in Crohn's disease (CD). (ebscohost.com)
  • IL-12 chimeric mice were produced by transplantation of WT bone marrow into IL-12 -/- mice to restore IL-12 production by immune cells, while leaving them deficient in renal IL-12 production. (asnjournals.org)
  • IL-12 chimeric mice showed no attenuation of their systemic cognate immune response to the nephritogenic antigen (sheep globulin), indicated by antigen-specific circulating antibody and cutaneous delayed-type hypersensitivity. (asnjournals.org)
  • These studies indicate that IL-12 produced by non-bone marrow derived intrinsic renal cells contributes to immune renal injury. (asnjournals.org)
  • The role of IL-12 in immune responses in vivo has been referred to as a "functional bridge" between the early noncognate innate resistance and subsequent antigen-specific adaptive immunity ( 13 , 16 , 17 ). (asnjournals.org)
  • Oxaliplatin cooperates with IL-12 in the stimulation of an efficient immune response against cancer cells, with complete eradication of pre-established colorectal cancer liver metastases and prevention of experimental relapses in a syngeneic model. (bmj.com)
  • These receptors allow NK cells to kill antibody-coated target cells, thus providing another form of immune defense ( 12 , 35 ). (asm.org)
  • Serial biopsy analyses from a phase 1 study demonstrate that transformation of tumor cells with electroporation (EP) of plasmid interleukin-12 (pIL-12) promotes this inflammatory immune milieu. (springer.com)
  • It is known that interleukin-12 (IL-12) plays a significant role in the activation of the Th1 immune response and, at the same time, that IL-10 action results in a negative regulation of this process ( 4 ). (asm.org)
  • In conclusion, our supplemental analysis shows that the IL-12/IL-10 ratio may be a useful way to approach immune status and its clinical significance in CCHF and, perhaps, other viral hemorrhagic fevers. (asm.org)
  • Various microbial stimuli induce the production of IL-12 by DCs in the early phase of the immune response. (biomedcentral.com)
  • In contrast, IL-12 gene knock-out in mice or a treatment of mice with IL-12 specific antibodies ameliorated the disease. (wikipedia.org)
  • The following lists a number of Interleukin 12 antibodies from various sources. (biocompare.com)
  • Your search returned 1221 Interleukin 12 Antibodies across 43 suppliers. (biocompare.com)
  • In addition, treatments with antibodies neutralizing IL-12 do not significantly modify the prominent late T cell CTL and IFN-γ responses during LCMV infection ( 12 ). (pnas.org)
  • In animals treated with rabbit polyclonal antibodies specific for IP-10 and Mig, the IL-12-induced regression of RENCA tumors was partially abrogated. (bloodjournal.org)
  • Mice were immunised with a plasmid encoding rbo IL-12 and boosted with rbo IL-12 and a number of monoclonal antibodies (mAb) were generated that reacted with rbo IL-12 in an ELISA. (ox.ac.uk)
  • Intracytoplasmic IL-12 was detected in bovine DC using the antibodies described herein. (ox.ac.uk)
  • Antibodies against IFN-gamma when given together with the priming agent, prevented the lethal reaction in mice primed with either LPS, IL-12, or IFN-gamma. (rupress.org)
  • Taken together, the studies suggest that IL-12 responses are differentially regulated during particular viral infections, and that IL-12-induced effects under the conditions of viral infections are distinct from those observed during other challenges. (pnas.org)
  • These data suggest that IL-12 and rituximab is an active combination and further studies of this combination in B-cell non-Hodgkin lymphoma are warranted. (bloodjournal.org)
  • We suggest that IL-12- and TNF-alpha-induced IFN-gamma production may contribute to the natural immunity against microorganisms in the CSF compartment during the acute phase of bacterial meningitis. (eur.nl)
  • These data suggest that IL-12, either induced by STAg or injected as a recombinant protein, mediates protection from ECM-associated pathology potentially through early induction of IFN-γ and reduction in parasitemia. (blogspot.com)
  • Studies in murine collagen-induced arthritis, an animal model of RA, suggest that IL-12 can promote arthritis in vivo . (biomedcentral.com)
  • Dr. Algazi and his team developed a platform that involves injection of the plasmid for IL-12 into the tumor and the application of electric current (ie, electroporation) to facilitate its uptake and sustained expression of IL-12. (ascopost.com)
  • Publications] Nagayama, H.: 'IL-12 responsiveness and expression of IL-12 receptor β1'Journal of Immunology. (nii.ac.jp)
  • Methods A high-capacity ( 'gutless' ) adenoviral vector carrying a liver-specific, mifepristone (Mif)-inducible system for the expression of IL-12 (HC-Ad/RUmIL-12) was used in combination with chemotherapy. (bmj.com)
  • Conclusions Long-term controlled expression of IL-12 using an HC-Ad vector in combination with oxaliplatin is effective and clinically applicable against hepatic colon cancer metastases. (bmj.com)
  • Results published in the Journal of Allergy and Clinical Immunology from a study where mice that were bred to be allergic to peanuts, interleukin-12 has been shown to not be present, suggesting that the molecule normally stops allergies to food developing. (wikipedia.org)
  • Furthermore, during infections of mice with lymphocytic choriomeningitis virus (LCMV), this treatment revealed a previously undetected early IL-12 and IFN-γ protein expression, and mice deficient in IFN-α/β receptor function, but not control mice, also expressed endogenous LCMV-induced IL-12. (pnas.org)
  • Studies from this laboratory evaluating endogenous expression and function of IL-12 indicate that the factor is differentially regulated in contrasting viral infections of mice. (pnas.org)
  • Interleukin 12 and tumor necrosis factor alpha are costimulators of interferon gamma production by natural killer cells in severe combined immunodeficiency mice with listeriosis, and interleukin 10 is a physiologic antagonist. (pnas.org)
  • We have demonstrated that a single injection of interleukin (IL)-12 on the day of bone marrow transplantation (BMT) inhibits acute graft-versus-host disease (GVHD) in mice. (jci.org)
  • To determine the mechanism by which IFN-gamma affects IL-12-mediated GVHD protection, we have compared the effect of IL-12 on GVHD in C57BL/6 wild-type (WT) or IFN-gamma gene knockout (GKO) recipients of fully major histocompatibility complex plus minor antigen-mismatched allogeneic BMT from WT or GKO BALB/c mice. (jci.org)
  • T-cell migration was inhibited by injection of anti-IFN-γ mAb to OV-HM tumor-bearing mice prior to IL-12 treatment. (aacrjournals.org)
  • Spleen cells from IL-12-treated or untreated OV-HM-bearing mice were stained in vitro with a fluorescein chemical and transferred i.v. into OV-HM-bearing mice that were not treated with IL-12. (aacrjournals.org)
  • Compared to spleen cells from OV-HM-bearing mice that were not treated with IL-12, enhanced migration was observed for cells from IL-12-treated OV-HM-bearing mice. (aacrjournals.org)
  • Anti-IFN-γ pretreatment of donor mice before IL-12 treatment did not reduce the migratory capacity of T cells, whereas migration was markedly inhibited in recipient mice injected with anti-IFN-γ. (aacrjournals.org)
  • Single low-dose cyclophosphamide combined with interleukin-12 gene therapy is superior to a metronomic schedule in inducing immunity against colorectal carcinoma in mice. (sigmaaldrich.com)
  • We previously reported a potent antitumor effect of a single low-dose cyclophosphamide and interleukin-12 (IL-12) gene therapy against advanced gastrointestinal carcinoma, in mice. (sigmaaldrich.com)
  • The role of the chemokines IP-10 and Mig (monokine induced by IFN-γ) in antitumor activity induced by systemic treatment with IL-12 was examined in mice bearing the murine renal adenocarcinoma RENCA, the CSA1M fibrosarcoma, and the OVHM ovarian carcinoma. (bloodjournal.org)
  • The ability of interleukin (IL)-12 to prevent tumors when administered to individuals with a genetic risk of cancer was studied in two lines of transgenic mice expressing rat HER-2/ neu oncogene in the mammary gland. (rupress.org)
  • the first course with 50 ng IL-12/day, the second with 100 ng IL-12/day) begun at 2 wk of age in BALB/c mice and at 21 wk of age in FVB mice markedly delayed tumor onset and reduced tumor multiplicity. (rupress.org)
  • Transgene-carrying males were backcrossed with BALB/c females for 12 generations and HER-2/ neu + BALB/c mice (BALB-NeuT) were used in these experiments. (rupress.org)
  • Established murine adenocarcinoma (CT26) and renal cell carcinoma (Renca) tumors in BALB/c mice were treated by direct intratumoral injection of a nonviral plasmid DNA vector encoding the murine IL-12 ( mIL-12 ) gene, either alone (naked) or in complexes with cationic lipid. (aacrjournals.org)
  • The marked reduction of tumor growth in tumor-bearing mice treated with mIL-12 cDNA was associated with the augmentation of tumor-specific cytotoxic T cells, enhanced production of IFN-γ in spleen and lymph node cells, and increased splenomegaly and lymphadenopathy. (aacrjournals.org)
  • CD4 + ratio in tumor-infiltrating lymphocytes was significantly increased in the tumor-bearing mice treated with mIL-12 DNA alone and mIL-12 cDNA plus lipid as compared with a control vector-treated group. (aacrjournals.org)
  • 1991). The importance of IL-12 for this loop is demonstrated by IL-12 and STAT4 knockout mice that are severely compromised in IFN-gamma production (Kaplan et al. (reactome.org)
  • In TNBS colitic mice, IL-12(p40)-IgG2b decreased mortality (10% v 68%), prevented body weight loss, reduced tumour necrosis factor α, and increased IL-10 secretion. (ebscohost.com)
  • To address this possibility, the development of sheep anti-mouse glomerular basement membrane globulin-induced crescentic GN was studied in C57BL/6 wild-type (WT), IL-12-deficient (IL-12 -/-), and IL-12 "chimeric" mice. (asnjournals.org)
  • IL-12 -/- mice showed significant protection from GN. (asnjournals.org)
  • Chimeric IL-12 mice showed significant attenuation of crescent formation, glomerular T-cell and macrophage accumulation, and renal impairment, compared with WT and sham chimeric mice, but were not protected to the same extent as IL-12 -/- mice. (asnjournals.org)
  • IL-12 has been demonstrated in crescentic glomeruli of mice that are susceptible to autoimmune anti-glomerular basement membrane (anti-GBM)-induced GN but was not detectable in strains that are resistant to the development of crescentic glomerular lesions ( 6 ). (asnjournals.org)
  • In the autoimmune GN associated with the "lupus like" syndrome of MRL/ Fas 1pr mice, intrarenal IL-12 expression by infiltrating mononuclear cells and tubular epithelial cells was demonstrated in association with increased IFN-γ mRNA ( 18 ). (asnjournals.org)
  • Huang, F.P. , Feng, G.J. , Lindop, G. , Stott, D.I. and Liew, F.Y. (1996) The role of interleukin 12 and nitric oxide in the development of spontaneous autoimmune disease in MRL/MP-lpr/lpr mice. (gla.ac.uk)
  • It is interesting to note that peritoneal cells from MRL/lpr mice also produced markedly higher concentrations of interleukin (IL) 12 than those from MRL/+ or BALB/c mice when cultured with same stimuli. (gla.ac.uk)
  • Serum from MRL/lpr mice contained significantly higher concentrations of IL-12 compared with those of MRL/+ or BALB/c control mice. (gla.ac.uk)
  • Daily injection of recombinant IL-12 led to increased serum levels of IFN- gamma and NO metabolites, and accelerated glomerulonephritis in the young MRL/lpr mice (but not in the MRL/+ mice) compared with controls injected with phosphate-buffered saline alone. (gla.ac.uk)
  • The injection of interferon gamma (IFN-gamma) or interleukin 12 (IL-12) instead of the LPS priming injection induced the lethal reaction in mice further challenged with LPS. (rupress.org)
  • If mixtures of TNF and IL-1 or mixtures of TNF and IFN-gamma are used to challenge mice previously primed with IFN-gamma or IL-12, mortality is induced. (rupress.org)
  • Clarithromycin significantly improves interleukin-12-mediated anti-Mycobacterium avium activity and abolishes toxicity in mice. (oregonstate.edu)
  • Therefore, we hypothesized that the combination of a model nonsteroidal anti-inflammatory drug - indomethacin and interleukin 12 would result in enhanced angiogenesis-dependent antitumor effects against a colon-26 carcinoma cells transplanted into syngeneic mice. (elsevier.com)
  • Using IL-10 and IL-12βR deficient mice, we found that STAg-induced protection from ECM is IL-10-independent but IL-12-dependent. (blogspot.com)
  • Treatment of P. berghei infected mice with recombinant IL-12 significantly decreased parasitemia and mortality. (blogspot.com)
  • We demonstrate that IFN-gamma production from SCID splenocytes is stimulated by interleukin (IL) 12, tumor necrosis factor alpha (TNF-alpha), and IL-2 but is inhibited by IL-10, IL-10, IL-12, and TNF are induced by heat-killed Listeria monocytogenes (hk-LM) from SCID splenocytes and peritoneal macrophages. (pnas.org)
  • Interleukin-12 secretion by Mycobacterium tuberculosis-infected macrophages. (asm.org)
  • Infection with Mycobacterium tuberculosis or phagocytosis of large latex beads induced interleukin-12 (IL-12) production in macrophages. (asm.org)
  • Interleukin 12 (IL-12) is a regulatory protein produced by activated B lymphocytes and macrophages. (sbhsciences.com)
  • Cytotoxicity in glioma cells due to interleukin-12 and interleukin-18-stimulated macrophages mediated by interferon-gamma-regulated nitric oxide. (virtualtrials.com)
  • I nduction of interleukin-12 production in mouse macrophages by berberine deviates CD4+ T cells from a Th2 to a Th1 response. (lifecarechiropractic.com)
  • IL-2 stimulates the expression of two IL-12 receptors, IL-12R-β1 and IL-12R-β2, maintaining the expression of a critical protein involved in IL-12 signaling in NK cells. (wikipedia.org)
  • The protein encoded by this gene is a type I transmembrane protein identified as a subunit of the interleukin 12 receptor complex. (nih.gov)
  • The target antigen refers to the Interleukin 12 protein complex, which comprises multiple genes. (biocompare.com)
  • IL-12 has demonstrated remarkable antitumor activity when used directly as a recombinant protein or when different viral or non-viral vectors transfer its genes. (cun.es)
  • Neutralization of IFN-α/β expressed endogenously during infections with murine cytomegalovirus (MCMV) enhanced early IL-12 and IFN-γ protein production. (pnas.org)
  • Interleukin-12 (IL-12) is a heterodimeric protein, first recovered from EBV-transformed B cell lines. (aacrjournals.org)
  • Interleukin-12 is a 70 kDa protein that is composed of covalently linked 40 kDa and 35 kDa subunits. (umassmed.edu)
  • Interleukin-12 is a disulfide-linked heterodimeric protein (p70), composed of two subunits, p35 and p40, which are encoded by two different genes. (sbhsciences.com)
  • Recombinant human IL-12 p40 is a 40 kDa protein containing 306 amino acid residues. (sbhsciences.com)
  • The lyophilized protein is stable for a few weeks at room temperature, but best stored at -80 0 C. Reconstituted IL-12 p40 should be stored in working aliquots at -80 0 C. (sbhsciences.com)
  • A greater than 20-fold increase in the serum levels of IFN-γ and a 2.5- to 5-fold increase in inducible protein 10 (IP-10) levels was seen at IL-12 doses of 100 ng/kg or greater. (bloodjournal.org)
  • 3-6 A further activity of IL-12 that may contribute to an antitumor effect is the ability to inhibit angiogenesis, an effect mediated through the induction of IFN-γ and inducible protein 10 (IP-10). (bloodjournal.org)
  • Human IL-12 is a disulfide linked heterodimeric protein with a molecular weight of 70 kDa comprised of disulfide-bonded 35 kDa (p35) and 40 kDa (p40) subunits. (sbhsciences.com)
  • Local and systemic administration of IL-12 protein has been studied in various murine models ( 4 , 5 , 12 , 21 ) and in Phase I/II human trials ( 22 , 23 ). (aacrjournals.org)
  • However, IL-12 protein therapy has been limited by dose-dependent toxicity ( 24 , 25 ). (aacrjournals.org)
  • The present study evaluated the protective effects of intranasally administered live L. lactis strains carrying human papillomavirus 16 E7 protein and murine interleukin‑12 (IL‑12) DNA (LL‑E7P‑IL‑12D) in a TC‑1 tumor animal model. (spandidos-publications.com)
  • Methods: We generated a fusion protein consisting of the IL-12(p40) subunit fused to the constant region of IgG2b. (ebscohost.com)
  • Results: Dimeric IL-12(p40)-IgG2b fusion protein bound specifically to the IL-12 receptor. (ebscohost.com)
  • Conclusions: The IL-12(p40)-IgG2b fusion protein has dichotomic properties as a specific IL-12 antagonist and selective repressor of mucosal inflammation at low concentration and as an IL-12 agonist at high concentration. (ebscohost.com)
  • Despite evidence indicating its biological effect in humans, neither the recombinant protein nor gene therapy vectors expressing IL-12 have shown a relevant benefit in patients with cancer. (bmj.com)
  • This protein binds to interleukine 12 (IL12) with a low affinity, and is thought to be a part of IL12 receptor complex. (mybiosource.com)
  • This inhibition of IL-12 protein production was associated with a proportionate decrease in IL-12 p35 and p40 mRNA accumulation. (elsevier.com)
  • IL-11 did not similarly regulate monocyte/macrophage production of IL-8 or macrophage inflammatory protein-1α (MIP-1α) and IL-6 did not similarly inhibit IL-12 elaboration. (elsevier.com)
  • This protein is a receptor for interleukin 8 (IL8). (wikipedia.org)
  • Interleukin 24 (IL-24) is a protein that in humans is encoded by the IL24 gene . (wikipedia.org)
  • This interleukin is also known as melanoma differentiation-associated 7 ( mda-7 ) due to its discovery as a tumour suppressing protein. (wikipedia.org)
  • The cDNAs for the p40 ( Il12b ) and p35 ( Il12a ) genes for IL-12 were linked by a sequence encoding a 15-amino-acid (Gly 4 Ser) 3 flexible linker and inserted into a MSGV-1-based retroviral vector with a woodchuck hepatitis virus posttranscriptional regulatory element to help increase transgene expression. (aacrjournals.org)
  • This antibody recognizes Interleukin-12B (IL12B). (acris-antibodies.com)
  • Western blot analysis in CEM,MDA-MB453 cell line lysates (35ug/lane) using Interleukin-12 beta/IL12B antibody Cat. (acris-antibodies.com)
  • Flow cytometric analysis of MDA-MB435 cells (right histogram) compared to a negative control cell (left histogram) using Interleukin-12 beta/IL12B antibody Cat. (acris-antibodies.com)
  • Interleukin-12 (IL-12) is a heterodimer of interleukin-12 subunit alpha (IL12A, IL-12p35) and interleukin-12 subunit beta (IL12B, IL-12p40). (reactome.org)
  • It is encoded by the genes Interleukin-12 subunit alpha (IL12A) and Interleukin-12 subunit beta (IL12B), which encode a 35-kDa light chain (p35) and a 40-kDa heavy chain (p40), respectively. (reactome.org)
  • 1996). The receptor for IL-12 is a heterodimer of IL-12Rbeta1 (IL12RB1) and IL-12Rbeta2 (IL12RB2), both highly homologous to Interleukin-6 receptor subunit beta (IL6ST,gp130). (reactome.org)
  • These are important in activating critical transcription factor proteins such as STAT4 that are implicated in IL-12 signaling in T cells and NK cells. (wikipedia.org)
  • Interferon (IFN)-α/β-mediated negative regulation of interleukin 12 (IL-12) and IFN-γ proteins is reported here. (pnas.org)
  • Since IL-12 p40 dimers act as IL-12 antagonists, we assayed p40 dimer proteins to modulate chronic intestinal inflammation. (ebscohost.com)
  • Gene transfer methods are designed to confine IL-12 production in the tumor environment preventing systemic toxicity. (cun.es)
  • Encouraging pilot clinical results have been recently obtained from the first phase I trial studying adenovirus mediated in vivo gene transfer of IL-12 into lesions of advanced cancer patients. (cun.es)
  • In this study, we examined the effects of delivering IL-12 directly to the tumor site by adoptively transferring tumor-specific gene-engineered CD8 + T cells. (aacrjournals.org)
  • Here, we assessed whether the delivery of IL-12 by gene therapy together with metronomic cyclophosphamide exerts antitumor effects in a murine model of colorectal carcinoma. (sigmaaldrich.com)
  • 50 days by mIL-12 gene therapy rejected a subsequent challenge of parental tumor cells but not of an unrelated, syngeneic tumor. (aacrjournals.org)
  • These results indicate that direct intratumoral gene transfer with naked nonviral IL-12 DNA provides an effective and simple method for the treatment of murine tumors, suggesting an approach for clinical application. (aacrjournals.org)
  • Several methods to deliver the IL-12 gene, including particle-mediated gene transfer, have been studied in animal models ( 2 , 5 , 28 , 29 ). (aacrjournals.org)
  • Initial IL-12 gene therapy experiments were focused on retroviral vectors ( 30 , 31 ). (aacrjournals.org)
  • Analysis of tissue samples from patients treated with pIL-12 EP showed a gene expression pattern consistent with downstream activation of NK cells and interferon-γ-dependent genes, including key genes responsible for tumor inflammation, antigen processing and presentation (APM). (springer.com)
  • Mutations in this gene impair the development of interleukin-17-producing T lymphocytes and result in increased susceptibility to mycobacterial and Salmonella infections. (mybiosource.com)
  • Nuclear run-on assays revealed comparable decreases in IL-12 p35 and p40 gene transcription. (elsevier.com)
  • abstract = "We found previously that bovine lymph node cells (LNC) incubated with 12- O-tetradecanoylphorbol-13-acetate (TPA) for 18 h proliferate only to a limited degree on subsequent stimulation with concanavalin A (Con A), or with the comitogenic combination of Con A plus TPA. (elsevier.com)
  • Interleukin-12 has also shown antitumor activity in a number of in vivo murine tumor models, 6 8 and the antitumor effects were demonstrated in microscopic disease models as well as animals bearing large established tumors. (bloodjournal.org)
  • Determined by the stimulation of IFN-gamma production by murine splenocytes co-stimulated with IL-12. (sbhsciences.com)
  • DC-expressing IL-12 injected into the malignant tissue eradicated 50-100% well established malignant nodules derived from the injection of two murine colon adenocarcinoma cell lines. (cun.es)
  • IL-12(p40)-IgG2b was tested in a murine 2,4,6,-trinitrobenzene sulphonic acid (TNBS) colitis model and in lamina propria mononuclear cells (LPMNC) from patients with CD in vitro. (ebscohost.com)
  • Targeting IL-12/IL-23 by Employing a p40 Peptide-Based Vaccine Ameliorates TNBS-Induced Acute and Chronic Murine Colitis. (ebscohost.com)
  • Interleukin-12 (IL-12) directs the cognate nephritogenic T helper type 1 responses that initiate renal injury in murine crescentic glomerulonephritis (GN). (asnjournals.org)
  • We assessed the ability of IL-12 to facilitate hematopoietic recovery from radiation (625 rad) and chemotherapy (cyclophosphamide) in two tumor-bearing murine models, namely the EL4 lymphoma and the Lewis lung cancer models. (biomedcentral.com)
  • In contrast with the significant antitumor and antimetastatic activity of IL-12, documented in several preclinical studies, clinical trials with IL-12, used as a single agent, or as a vaccine adjuvant, have shown limited efficacy in most instances. (aacrjournals.org)
  • In contrast, IL-10 inhibits hk-LM-induced IFN-gamma production at two levels: (i) by inhibiting TNF and IL-12 production from these cultures (presumably from the macrophage) and (ii) by inhibiting the stimulatory effects of IL-12 and TNF-alpha on NK-cell IFN-gamma production. (pnas.org)
  • Tissue-specific, drug-inducible expression systems can be used to modulate the expression of transgenes in specific organs, but the biological effects of IL-12 can interfere with their functions. (bmj.com)
  • Induction of IFN-gamma, considered a key mediator of antitumor effects of IL-12, is known to decline on repeated administrations. (eur.nl)
  • We investigated the ability of interleukin-2 (IL-2) and IL-12 to enhance the cytotoxicity of neonatal (cord blood) and adult mononuclear cells (MNCs) in both natural killer (NK) cell and antibody-dependent cellular cytotoxicity (ADCC) assays. (asm.org)
  • Newborn mononuclear cells (MNCs) have decreased antibody-dependent cellular cytotoxicity (ADCC) compared to the ADCC of MNCs from adults ( 11 , 12 ), and newborns have decreased numbers of NK cells with both CD16 and CD56, a phenotype associated with greater cytotoxicity ( 32 ). (asm.org)
  • The skewed balance between IL-10 and IL-12 is associated with their capability to induce T-cell hyporesponsiveness, because a neutralizing antibody to IL-10, exogenous recombinant IL-12 or lipopolysaccharide (LPS) significantly blocked the hyporesponsiveness. (eurekamag.com)
  • The enhanced NO synthesis induced by IFN- gamma/LPS was substantially inhibited by anti-IL-12 antibody. (gla.ac.uk)
  • In addition, IL-12-induced NO production can also be markedly inhibited by anti-IFN-gamma antibody, but only weakly inhibited by anti-tumor necrosis factor alpha antibody. (gla.ac.uk)
  • Interleukin-12 and its role in coordinating antibody-dependent cell-mediated cytotoxicity is used illustrate the different time and length scale that underpin cancer immunoediting. (biomedcentral.com)
  • citation needed] IL-12 binds to the IL-12 receptor, which is a heterodimeric receptor formed by IL-12Rβ1 and IL-12Rβ2. (wikipedia.org)
  • Biologically active mouse IL-12 is a disulfide-linked, 70 kDa (p70) heterodimeric glycoprotein composed of a 40 kDa (p40) subunit and a 35 kDa (p35) subunit. (acris-antibodies.com)
  • Mutations in the genes for IFN-gammaR1, IFN-gammaR2, IL-12Rbeta1, and other molecules implicated in IL-12- or IFN-gamma-mediated immunity were sought. (jci.org)
  • Subsequent studies indicated that IL-12 could boost the generation of cytotoxic T cells by promoting the transcription of genes encoding cytolytic factors including perforin and granzymes ( 11 ). (aacrjournals.org)
  • We also present additional safety and more detailed biomarker data demonstrating the promotion of pro-inflammatory genes with pIL-12 EP therapy. (springer.com)
  • pIL-12 EP promotes the expression of pro-inflammatory genes including genes required for antigen processing and presentation. (springer.com)
  • Thus, it appears that IL-12-dependent, T-cell-mediated antitumor activity requires the intermediate expression of IP-10 and Mig to recruit antitumor effector T cells to the tumor site. (bloodjournal.org)
  • We compared the level of transgene expression and antitumor activity of two different interleukin (IL)-12 DNA preparations (naked DNA versus DNA lipid complex). (aacrjournals.org)
  • In this study production levels of interleukins (IL)-12 and IL-13 were measured by commercial ELISA in culture supernatants of mitogen-stimulated peripheral blood mononuclear cells from 30 non-splenectomized beta-thalassaemia cases with iron overload and 20 age- and sex-matched healthy individuals. (who.int)
  • Dans la présente étude, les taux de production des interleukines 12 et 13 (IL-12 et IL-13) ont été mesurés à l'aide de la méthode ELISA dans les surnageants de culture de cellules mononucléées de sang périphérique stimulées par des mitogènes chez 30 cas de bêta-thalassémie non splénectomisés présentant une surcharge martiale ainsi que chez 20 individus en bonne santé appariés pour l'âge et le sexe. (who.int)
  • A pair of mAb was identified that together could be used to detect both recombinant and natural bovine IL-12 by ELISA and a luminometric detection method was applied to the ELISA making it more sensitive. (ox.ac.uk)
  • The ELISA was also able to detect recombinant ovine IL-12 and, less effectively, recombinant human IL-12. (ox.ac.uk)
  • In contrast, bovine IL-12 was not detected by a commercial human IL-12 ELISA kit. (ox.ac.uk)
  • IL-12 ELISA Kit allows for the in vitro quantitative determination of IL-12 concentrations in plasma, tissue homogenates and other biological fluids. (medicobio.com)
  • IL-12 treatment of T H 2 cells expressing functional IL-12 receptors did not induce the conversion of these cells into the T H 1 phenotype if the cells were pretreated under T H 2 conditions. (sciencemag.org)
  • To enhance this effect artificially, bone marrow (BM)-derived DC were genetically engineered to produce high levels of functional IL-12 by ex vivo infection with a recombinant defective adenovirus (AdCMVIL-12). (cun.es)
  • This suggested that IL-12 is essential for protective immunity to intracellular bacteria such as mycobacteria and Salmonella. (wikipedia.org)
  • Interleukin-12 (IL-12) is recognized as a master regulator of adaptive type 1, cell-mediated immunity, the critical pathway involved in protection against neoplasia and many viruses. (aacrjournals.org)
  • Blocking of post-therapy iNOS activity with N-nitro-L-arginine methyl ester (L-NAME)dramatically enhanced tumor suppression revealing the inhibitory eVect of NO on IL-12-driven antitumor immunity. (uzh.ch)
  • Interleukin-12 (IL-12) plays a key role in linking innate immunity to an adaptive T helper-1 (Th1). (ebscohost.com)
  • This putative clinical function of IL-12 focuses on the dual role of IL-12 in hematopoiesis and immunity. (biomedcentral.com)
  • IL-12 and IL-23 form a crucial link to adaptive immunity through their ability to influence the development of TH1 and TH17 cells respectively. (nus.edu.sg)
  • Despite the importance of the TH1/IL-12 and TH17/IL-23 axis in protective immunity, excessive production of IL-12 and IL-23 can be a nuisance as it leads to immunopathology. (nus.edu.sg)
  • These experiments of nature demonstrate that IL-12-dependent IFNgamma production is critical for granuloma formation and therefore host immunity against such pathogens. (lshtm.ac.uk)
  • The mechanisms of antitumor action of interleukin 12 include the enforced secretion of anti-angiogenic factors and stimulation of antitumor immunity. (elsevier.com)
  • Chlorella supplementation had a beneficial immunostimulatory effect, enhancing the NK cell activity and produces interferon-γ and interleukin-12 as well as interleukin-1β. (greenmedinfo.com)
  • Interferon-gamma receptor ligand-binding chain (IFN-gammaR1) or signaling chain (IFN-gammaR2) deficiency, like interleukin 12 receptor beta1 chain (IL-12Rbeta1) deficiency, predispose to severe infections due to poorly virulent mycobacteria and salmonella. (jci.org)
  • Usage information: Donor-derived interferon gamma is required for inhibition of acute graft-versus-host disease by interleukin 12. (jci.org)
  • Donor-derived interferon gamma is required for inhibition of acute graft-versus-host disease by interleukin 12. (jci.org)
  • This effect of IL-12 can be diminished by anti-interferon (IFN)-gamma mAb. (jci.org)
  • The acute-phase, pretreatment plasma IL-12 and alpha interferon (IFN-α) levels, as well as the acute-phase mitogen-stimulated whole-blood production capacity of IL-12, were significantly lower in children with severe rather than mild malaria. (asm.org)
  • To assess the role of interleukin-12 (IL-12) and gamma interferon (IFN-gamma) in children with bacterial meningitis, bioactive IL-12 (p70) and the inactive subunit p40 and IFN-gamma were measured in serum and cerebrospinal fluid (CSF) from 35 children with bacterial meningitis and 10 control subjects. (eur.nl)
  • An immunological basis for these differences is demonstrated in that these animals produced interleukin-12 more rapidly and exhibited higher levels of gamma interferon earlier postinfection. (strath.ac.uk)
  • This chapter focuses on Inherited Disorders of the Interleukin- 12/23-Interferon Gamma Circuit, including the historic and scientific background, clinical presentations, immunologic characteristics, and the molecular/genetic underpinnings. (oxfordmedicine.com)
  • Heterogeneity in the granulomatous response to mycobacterial infection in patients with defined genetic mutations in the interleukin 12-dependent interferon-gamma production pathway. (lshtm.ac.uk)
  • We measured serum levels of IL-5, IL-10, IL-12, and interferon-gamma (IFN-γ) at diagnosis in AITL and other common subtypes of nodal T-cell lymphoma including peripheral T-cell lymphoma, not otherwise specified (PTCL-NOS), ALK-negative anaplastic large cell lymphoma (ALCL) or ALK-positive ALCL between September 2008 and December 2014. (readbyqxmd.com)
  • In addition, ATRA-DCs could drive T cells towards an IL-12-dependent T-helper cell type 1 response with secretion of interferon-gamma. (unige.it)
  • STAg treatment 24 hours post P. berghei infection led to a rapid increase in serum levels of interleukin (IL)-12 and interferon-γ (IFN-γ). (blogspot.com)
  • Peripheral blood mononuclear cells obtained from cancer patients before rhIL-12 therapy exhibited defective NK cell cytotoxicity and T-cell-proliferative responses. (aacrjournals.org)
  • Peripheral blood mononuclear cells obtained after lymphocyte recovery following the administration of a single 500 ng/kg dose of rhIL-12 displayed augmented NK cell cytolytic activity in four of four patients tested and enhanced T-cell proliferation in three of four patients tested. (aacrjournals.org)
  • Increased expression of interleukin-12 receptor β2 on lamina propria mononuclear cells of patients with active Crohn's disease. (ebscohost.com)
  • Since interleukin-12 is pathogenetically involved in Crohn's disease (CD) but not in ulcerative colitis (UC), expression and mechanisms of induction of interleukin-12 receptor (IL-12R) subunits β1 and β2 were analyzed in lamina propria mononuclear cells (LPMNC) of. (ebscohost.com)
  • The production of IFN-gamma is induced by IL-12 with tumor necrosis factor alpha (TNF-alpha) as a costimulator and inhibited by IL-10. (eur.nl)
  • On repeated rHuIL-12 injections, IL-10 concentrations increased further, whereas the transient increments of IFN-gamma, tumor necrosis factor-alpha, IL-6, and IL-8 concentrations, as well as the fluctuations of the leukocyte subset counts, were tapered. (eur.nl)
  • T and NK cells from seven unrelated patients who had severe idiopathic mycobacterial and Salmonella infections failed to produce IFNγ when stimulated with IL-12. (wikipedia.org)
  • Mutation analysis showed three previously reported mutations, two novel mutations in IL-12 R (beta1/beta2), and one previously reported mutation in IL-12 Mendelian Susceptibility to Mycobacterial Disease patients. (nih.gov)
  • Moreover, unacceptable toxicity resulting from IFN gamma overproduction was observed in 2 renal carcinoma patients included in a phase II clinical trial that consisted in systemic administration of rIL-12. (cun.es)
  • PURPOSE: Phase I trial to study the effectiveness of interleukin-12 in treating patients with cancer in the abdomen. (clinicaltrials.gov)
  • OBJECTIVES: I. Determine the maximum tolerated dose of intraperitoneal interleukin-12 in patients with Mullerian carcinoma (closed to accrual as of 8/23/01), gastrointestinal carcinoma, or peritoneal mesothelioma (closed to accrual as of 8/23/01). (clinicaltrials.gov)
  • Patients receive intraperitoneal interleukin-12 over 30 minutes once weekly for 4 weeks. (clinicaltrials.gov)
  • Patients receive escalating doses of intraperitoneal interleukin-12 until the maximum tolerated dose (MTD) is determined. (clinicaltrials.gov)
  • Once the MTD is established, additional patients are accrued to receive interleukin-12 at the recommended dose. (clinicaltrials.gov)
  • Our data suggested that IL-12 together with IL-2 caused a suppression of CD4 + CD25 + regulatory T-cells and an elevation of IFN-γ levels, which play a crucial immunotherapeutic role in the management of HCC patients. (dovepress.com)
  • Plasmid DNA encoding human interleukin 12 (IL-12) was produced under GMP conditions and injected into lesions of nine patients with malignant melanoma (stage IV) previously treated with both standard and nonstandard therapies. (nih.gov)
  • I. Determine the maximum tolerated dose of interleukin-12 when given in combination with paclitaxel and trastuzumab (Herceptin®) in patients with HER2/neu-overexpressing malignancies. (knowcancer.com)
  • Beginning with course 2, patients receive trastuzumab and paclitaxel as in course 1 and interleukin-12 subcutaneously on days 2, 5, 9, 12, 16, and 19. (knowcancer.com)
  • This phase I trial studies the side effects and best dose of pembrolizumab and recombinant interleukin-12 in treating patients with solid tumors. (centerwatch.com)
  • Patients receive recombinant interleukin-12 subcutaneously (SC) on days 2, 5, 9, and 12 and pembrolizumab intravenously (IV) over 30 minutes on day 8 of cycle 1 and day 1 of subsequent cycles. (centerwatch.com)
  • Patients then receive recombinant interleukin-12 SC on days 2, 5, 9, and 12. (centerwatch.com)
  • In patients with stage III/IV melanoma, immunologically "cold" tumors were rendered immunologically active through intratumoral injections of plasmid interleukin-12 (IL-12) combined with pembrolizumab (Keytruda). (ascopost.com)
  • Intratumoral delivery of plasmid interleukin-12 via electroporation led to a 40% to 48% response rate among 22 stage III/IV melanoma patients in a phase II study. (ascopost.com)
  • I. Determine the efficacy of interleukin-12 (IL-12) by evaluating its effect on remission duration after response to second line chemotherapy with ifosfamide and etoposide in patients with HIV-associated non-Hodgkin's lymphoma. (knowcancer.com)
  • Determine the safety of IL-12 when administered as maintenance therapy in these patients. (knowcancer.com)
  • V. Evaluate the effect of IL-12 on HIV viral load and on functional T-cell assays in these patients. (knowcancer.com)
  • Evaluate the effect of IL-12 on Epstein-Barr Virus (EBV) viral load in these patients. (knowcancer.com)
  • Patients who have complete or partial remission after a minimum of 4 courses of chemotherapy receive maintenance therapy consisting of interleukin-12 SC twice weekly beginning on day 28 of the final chemotherapy course and continuing for 6 months or until disease progression. (knowcancer.com)
  • This open-label Phase I study is designed to evaluate the safety and immunologic/microbiologic effects of interleukin-12 administration in HIV-infected patients with concomitant disseminated Mycobacterium avium (MAC) infection. (clinicalconnection.com)
  • Fifteen patients with documented disseminated MAC will be randomized to receive double-blinded placebo or escalating doses of IL-12 in addition to anti-MAC chemotherapy and standard anti-retroviral therapy for six weeks. (clinicalconnection.com)
  • Those patients receiving placebo after 6 weeks will be crossed over to receive the full treatment course of IL-12. (clinicalconnection.com)
  • Objective responses occurred in 29 of the 43 patients (69%), with 8 of 11 complete responses seen at IL-12 doses of 300 ng/kg or greater. (bloodjournal.org)
  • Forty patients with advanced cancer received bolus i.v. injections of rhIL-12 in doses ranging between 3 and 1000 ng/kg. (aacrjournals.org)
  • These studies confirm that doses of rhIL-12 resulting in significant immunological activity can be administered with acceptable toxicity to cancer patients. (aacrjournals.org)
  • In contrast to CSF levels of TNF-alpha, IL-12, and IL-10, those of IFN-gamma were significantly higher in patients with pneumococcal meningitis than in children with meningitis caused by Haemophilus influenzae and Neisseria meningitidis, presumably because of a high CSF TNF-alpha/IL-10 ratio in the former. (eur.nl)
  • PURPOSE: Repeated administrations of recombinant human interleukin-12 (rHuIL-12) to cancer patients are characterized by a reduction of side effects during treatment. (eur.nl)
  • EXPERIMENTAL DESIGN: In a Phase I study of 26 patients with advanced renal cell cancer, rHuIL-12 was administered s.c. on day 1, followed by 7 days rest and six injections administered over a 2-week time period. (eur.nl)
  • Here we present clinical response data for 30 advanced melanoma patients (pts) treated with pIL-12 EP in a phase 2 trial (OMS-I100). (springer.com)
  • Thirty patients with stage IIIB-IV melanoma received up to 4 cycles of pIL-12 EP into superficial cutaneous, subcutaneous, and nodal lesions on days 1, 5 and 8 of each 12-week cycle. (springer.com)
  • Since several patients had undetectable IL-12 levels (value = 0), we used the ratio of IL-12/IL-10 to avoid division by zero. (asm.org)
  • Comparison of IL-12/IL-10 concentration ratio and clinical course/outcome in patients with acute CCHF. (asm.org)
  • Correlation between IL-12/IL-10 concentration ratio and viral load in patients with acute CCHF. (asm.org)
  • IL-12 combination with other immunotherapy approaches synergizes to achieve even better results. (cun.es)
  • Recombinant Interleukin-12 for Immunotherapy: A Double-Sided Sword. (ebscohost.com)
  • Methods to control the location, intensity and duration of IL-12 production are needed to increase the safety and efficacy of genetic immunotherapy. (bmj.com)
  • The promising data obtained in the preclinical models of antitumor immunotherapy raised hope that IL-12 could be a powerful therapeutic agent against cancer. (biomedcentral.com)
  • Platinum Eco 293 based cells (Cell Bio Labs) were plated on poly- d -lysine-coated 100-mm plates (BD Biosciences) and transfected with 6 μg of pCL-Eco helper plasmid (Imgenex) and 9.3 μg of the MSGV-1 IL-12 or the MSGV-1 pmel-1 TCR vector with Lipofectamine 2000 (Invitrogen) overnight in antibiotic-free complete medium. (aacrjournals.org)
  • Intratumoral plasmid IL-12 delivered via electroporation (IT-pIL12-EP) has been shown to increase tumor-infiltrating lymphocytes in both treated and untreated lesions. (ascopost.com)
  • Safety and comparative immunogenicity of an HIV-1 DNA vaccine in combination with plasmid interleukin 12 and impact of intramuscular electroporation for delivery. (harvard.edu)
  • Extended studies with IFN-α demonstrated that inhibition was at the level of biologically active IL-12 p70. (pnas.org)
  • Moreover, the effective inhibition of host-reactive donor T cell activation and expansion that is associated with IL-12-mediated GVHD protection was dependent on the ability of BALB/c donors to produce IFN-gamma. (jci.org)
  • Johnsson, H.J. and McInnes, I.B. (2015) Interleukin-12 and interleukin-23 inhibition in psoriatic arthritis. (gla.ac.uk)
  • Hence, our study has revealed the importance of TTP as a negative regulator of inflammatory dendritic cell function through the suppression of excessive IL-12 and IL-23 production and the inhibition of their TH1 polarizing potential. (nus.edu.sg)
  • As administration of interleukin 12 and indomethacin resulted in enhanced inhibition of angiogenesis it seems possible that prevention of new blood vessel formation is one of the mechanisms responsible for the observed antitumor effects. (elsevier.com)
  • Oral administration of Lactobacillus acidophilus may upregulate IL-12 production which may have a therapeutic activity against breast tumors. (greenmedinfo.com)
  • As a result, IL-12 can eliminate several types of tumors developed in rodents. (cun.es)
  • We found that transferring small numbers of IL-12-engineered T cells into lymphodepleted hosts resulted in the destruction of large vascularized tumors without the need for vaccine or IL-2. (aacrjournals.org)
  • Accumulating data indicate that p40 secretion precedes that of IL-12 expression. (sbhsciences.com)
  • 10-6 M, IL-12(p40)-IgG2b increased IFN-γ secretion and lymphocyte proliferation thereby acting as on IL-12 agonist. (ebscohost.com)
  • The lack of proliferation was traced to a lack of secretion of interleukin 2 (IL-2). (elsevier.com)
  • An extensive review and visualization of IL-12 signaling can be found at the peer-reviewed pathway database Reactome: Interleukin-12 family IL-12 is linked with autoimmunity. (wikipedia.org)
  • These results demonstrate that (a) acute GVHD can be induced in the absence of IFN-gamma, (b) host IFN-gamma does not play a critical role in IL-12-induced GVHD protection, and (c) the protective effect of IL-12 against GVHD is dependent on the ability of the donor to produce IFN-gamma. (jci.org)
  • However, excessive clinical toxicity, largely due to repeat dose regimens, and modest clinical response observed in the clinical trials have pointed to the necessity to design protocols that minimize toxicity without affecting the anti-tumor effect of IL-12. (biomedcentral.com)
  • The effect of IL-12 on NO production was dependent on the presence of natural killer and possibly T cells. (gla.ac.uk)
  • These results describe a systemic approach to achieve high local concentrations of IL-12, leading to dramatic improvements in adoptive cell therapies. (aacrjournals.org)
  • CSF concentrations of IL-12 p40 as well as those of IFN-gamma were markedly elevated, whereas IL-12 p70 was hardly detectable. (eur.nl)
  • OUTLINE: This is a dose-escalation study of interleukin-12. (knowcancer.com)
  • Moreover, endogenous IFN-α/β induced by LCMV inhibited in vivo lipopolysaccharide stimulation of IL-12 production. (pnas.org)
  • CD2, LFA-1, and CD56 were transiently up-regulated on the surface of NK cells exposed to rhIL-12 in vivo . (aacrjournals.org)
  • These data suggest that CD40-deficient DC producing IL-10, but not IL-12 can induce T-cell hyporesponsiveness in vitro and in vivo. (eurekamag.com)
  • The aim of this study was to investigate the mechanisms exerted by complexed linear DNA vectors encoding Interleukin (IL-) 12 and IL-18 applied in vivo in horses. (frohberg.de)
  • Publications] Kawashima, T.: 'Interleukin-12 omdices tyrosine phosphorylation'Cellular Immunology. (nii.ac.jp)
  • IL-12 plays an important role in the activities of natural killer cells and T lymphocytes. (wikipedia.org)
  • IL-12 mediates enhancement of the cytotoxic activity of NK cells and CD8+ cytotoxic T lymphocytes. (wikipedia.org)
  • Support is lent to this idea by the observation that a receptor for IL-12 is important for IFNγ production by lymphocytes. (wikipedia.org)
  • The isolated lymphocytes were cultured in the presence of interleukins (IL)-2, IL-4, and IL-12. (dovepress.com)
  • Mixed cell populations of peripheral blood lymphocytes and tumor infiltrating lymphocytes treated with IL-2 plus IL-12 showed a marked and significant elevation in IFN-γ levels in their culture media, a significant decrease in the percentage of cluster of differentiation (CD)4 + CD25 + regulatory T-cells, and a nonsignificant increase in the percentage of CD8 + cytotoxic T-cells. (dovepress.com)
  • Initial characterization of its biological activities revealed that IL-12, when added to human peripheral blood lymphocytes, induced IFN-γ production, increased NK cell cytotoxicity as well as T cell proliferation in response to mitogenic lectins and phorbol diesters (see ref. 11 for review). (aacrjournals.org)
  • IL-12 has been shown to have multiple effects on T lymphocytes and natural killer (NK) cells. (acris-antibodies.com)
  • 9 10 IL-12 treatment produced a potent antitumor effect that was associated with tumor infiltration by CD4 + and CD8 + T lymphocytes. (bloodjournal.org)
  • I. Establish the maximum tolerated dose (MTD) and the recommended phase II dose (RP2D) of recombinant human interleukin (rhIL)-12 in combination with MK-3475 (pembrolizumab). (centerwatch.com)
  • Measure CD8+ T cell infiltration by immunohistochemistry in tumor biopsies obtained at baseline, after one week of rhIL-12 and after 2 cycles of MK-3475 (pembrolizumab) in combination with rhIL-12. (centerwatch.com)
  • The immunological effects of recombinant human interleukin 12 (rhIL-12) administration were examined during the conduct of a Phase I clinical trial. (aacrjournals.org)
  • Dose-dependent increases in serum IFN-γ levels were seen during rhIL-12 therapy. (aacrjournals.org)
  • Significant lymphopenia was observed 24 h after single i.v. injections of rhIL-12 at each dose level. (aacrjournals.org)
  • The degree of lymphopenia was dose dependent, and a plateau effect was seen with rhIL-12 doses of 100 ng/kg and higher. (aacrjournals.org)
  • Lymphocyte counts reached nadir levels at approximately 10 h after rhIL-12 injection and returned to baseline within 14 days postinjection. (aacrjournals.org)
  • Rebound lymphocytosis, as seen after interleukin 2 therapy, was not observed after recovery from rhIL-12-induced lymphopenia. (aacrjournals.org)
  • rhIL-12-induced lymphopenia involved all major lymphocyte subsets, although natural killer (NK) cell numbers were the most profoundly affected, and CD4 T-cell numbers were the least affected. (aacrjournals.org)
  • Furthermore, rhIL-12 therapy can reverse defects in NK cell and T-cell function that are associated with advanced cancer in humans. (aacrjournals.org)
  • rhIL-12 was found to have substantial biological effects when administered in doses that were generally tolerable in an outpatient setting. (aacrjournals.org)
  • L-NAME as a potent adjuvant in IL-12 therapy of cancer. (uzh.ch)
  • IL-23 is more potent than IL-12 in inducing long-lasting T-cell responses to viral antigens and can induce tumor-directed cytotoxic T-cell responses. (biovendor.com)
  • These studies demonstrate that IL-11 is a potent inhibitor of monocyte/macrophage IL-12 production and that this inhibitory effect is, at least in part, transcriptionally mediated. (elsevier.com)
  • In addition, IL-12 inhibits tumor angiogenesis mainly through IFN gamma-dependent production of the chemokine IP10. (cun.es)
  • IL-12 production, but not IFN-gamma, was completely inhibited by the addition of anti-CD40L mAb. (nih.gov)
  • Both IFN-α and IFN-β inhibited fixed Staphylococcus aureus Cowan strain induction of IL-12 and IFN-γ production by mouse splenic leukocytes in culture. (pnas.org)
  • The effects of IFN-α/β neutralization on production of IL-12 and IFN-γ during the viral infections were detected in both serum samples and medium conditioned with splenic leukocytes isolated from infected animals. (pnas.org)
  • IL-12 production is necessary for hk-LM to stimulate IFN-gamma production by SCID splenocytes since neutralization of IL-12 totally blocks IFN-gamma production in this system. (pnas.org)
  • TNF-alpha and IL-2 act synergistically with IL-12 to augment IFN-gamma production. (pnas.org)
  • Thus, these data indicate that macrophage production of TNF-alpha and IL-12 stimulates the release of IFN-gamma by NK cells and that IL-10 produced in response to hk-LM inhibits this response at the level of the macrophage and the NK cell. (pnas.org)
  • Krauthausen M, Ellis SL, Zimmermann J, Sarris M, Wakefield D, Heneka MT, Campbell IL, Müller M. Opposing roles for CXCR3 signaling in central nervous system versus ocular inflammation mediated by the astrocyte-targeted production of IL-12. (umassmed.edu)
  • Interleukin 12 (IL12) can act as a growth factor for activated T and NK cells, enhance the lytic activity of NK/lymphokine activated killer cells, and stimulate the production of IFN gamma by resting PBMC. (acris-antibodies.com)
  • In particular, IFN gamma stimulates production of more IL-12 and sets up a positive regulation loop between IL-12 signaling and IFN gamma (Chan et al. (reactome.org)
  • PI3K-mediated negative feedback regulation of IL-12 production in DCs. (ebscohost.com)
  • The recent demonstration of IL-12 production by intrinsic renal cells, including mesangial and proximal tubular cells, raises the possibility that IL-12 from nonimmune cells may contribute to inflammatory renal injury. (asnjournals.org)
  • IL-12 primes CD4 + T cells for high IFN-γ production ( 14 , 15 ) and polarizes uncommitted T cells toward a Th1 profile. (asnjournals.org)
  • However production of IL-12 response to brucella antigenic in positive brucellosis cattle owners and negative brucellosis was increased in comparison to the health control, differences between the values was not significant. (sid.ir)
  • Analysis of flow cytometry data by a sensitive subtraction method allowed measurement of production of interleukin (IL)-12 and IL-10 by small numbers of gut DC by intracellular staining. (lancs.ac.uk)
  • Regulated IL-12 production is crucial for the generation of protective Th1 responses to infectious agents. (elsevier.com)
  • IL-11 did not alter the IL-12 (p70) production of unstimulated THP-1 monocytic cells or human blood monocytes. (elsevier.com)
  • It did, however, inhibit, in a dose-dependent fashion, the IL-12 production of IFN-γ plus Staphylococcus aureus Cowan strain 1-stimulated THP-1 cells and stimulated blood monocytes. (elsevier.com)
  • Elias, Jack A. / Interleukin-11 inhibits macrophage interleukin-12 production . (elsevier.com)
  • However, the regulation of IL-12 production in RA has not been elucidated. (biomedcentral.com)
  • IL-15, but not IL-12, significantly increased CD3+ CD8+ expression among the CB MNCs after 1 week of culture. (eurekamag.com)
  • IL-12 levels were significantly lower among cases compared with controls (91.4 pg/mL versus 154.6 pg/mL), while IL-13 levels were significantly higher (42.5 pg/mL versus 5.7 pg/mL). (who.int)
  • Recombinant interleukin-12 may kill tumor cells by blocking blood flow to the tumor and by stimulating white blood cells to kill tumor cells. (centerwatch.com)
  • The administration of oxaliplatin (5 mg/kg intraperitoneally) 3 days before starting the induction regimen achieved efficient elimination of liver metastases with a single cycle of IL-12 induction, and improved protection against tumour rechallenge. (bmj.com)
  • These results highlight the importance of early IL-12 induction in protection against ECM. (blogspot.com)
  • These results indicate that IFN-γ plays two distinct roles in expressing the antitumor efficacy of IL-12: one is to support the T-cell acceptability of tumor masses, and the other is to mediate the antitumor effects of migrated T cells. (aacrjournals.org)
  • We describe a child with interleukin-12 receptor beta1 (IL-12Rbeta1) deficiency caused by a homozygous IL12RB1 large deletion who presented at the age of 1 year with recurrent, often asymptomatic episodes of bacteremia caused by group D Salmonella species. (nih.gov)
  • IL12RB1: Functions as an interleukin receptor which binds interleukin-12 with low affinity and is involved in IL12 transduction. (mybiosource.com)
  • IL-12 and IFN-gamma productions were dose-dependent on antigens in PS-liposomes. (nih.gov)
  • IL-12 will be administered subcutaneously, with escalating doses every month over the dose range of 30 ng/kg, 100 ng/kg, and 300 ng/kg, or until an individual maximum tolerated dose (IMTD) is reached. (clinicalconnection.com)
  • A phase 1 study of IL-12 in combination with rituximab was conducted in 43 adults with B-cell lymphoma to determine the optimal immunologic dose of this combination. (bloodjournal.org)
  • Rituximab was administered at a dose of 375 mg/m 2 by intravenous infusion weekly for 4 weeks, and IL-12 was given subcutaneously twice weekly. (bloodjournal.org)
  • The starting dose of IL-12 was 30 ng/kg and this was escalated to 500 ng/kg. (bloodjournal.org)
  • Constitutional symptoms and liver enzyme elevations at 500 ng/kg of IL-12 were dose limiting. (bloodjournal.org)
  • The optimal immunologic dose of IL-12 in combination with rituximab was determined to be 300 ng/kg subcutaneously twice weekly starting on day 2. (bloodjournal.org)
  • Dose escalation of IL-12 within clinically tolerable margins did not reduce the decline of these immunological effects. (eur.nl)
  • SONN ) has a preclinical nonhuman primate GLP repeat-dose study of SON-1010, a proprietary version of Interleukin 12 (IL-12) configured using Sonnet's Fully Human Albumin Binding (FHAB) platform. (webull.com)
  • Some of these mAb neutralised the ability of rbo IL-12 to induce IFNgamma synthesis by bovine cells. (ox.ac.uk)
  • The mechanisms of antitumor action are complex, as IL-12 can directly augment the functionality of multiple end effectors such as CD4 + (type 1), CD8 + , and natural killer (NK) cells ( 12 , 21 - 25 ). (aacrjournals.org)
  • Successful therapy was dependent on IL-12 transfection and was mediated only by syngeneic, but not allogeneic BM-derived DC, indicating that compatible antigen-presenting molecules were required. (cun.es)
  • Although ATRA-DCs retained their antigen-capture capacity, they secreted interleukin (IL)-12p70 without the need for any maturation agent. (unige.it)