A soluble factor produced by MONOCYTES; MACROPHAGES, and other cells which activates T-lymphocytes and potentiates their response to mitogens or antigens. Interleukin-1 is a general term refers to either of the two distinct proteins, INTERLEUKIN-1ALPHA and INTERLEUKIN-1BETA. The biological effects of IL-1 include the ability to replace macrophage requirements for T-cell activation.
A soluble substance elaborated by antigen- or mitogen-stimulated T-LYMPHOCYTES which induces DNA synthesis in naive lymphocytes.
Cell surface proteins that bind interleukins and trigger intracellular changes influencing the behavior of cells.
A cytokine that stimulates the growth and differentiation of B-LYMPHOCYTES and is also a growth factor for HYBRIDOMAS and plasmacytomas. It is produced by many different cells including T-LYMPHOCYTES; MONOCYTES; and FIBROBLASTS.
Non-antibody proteins secreted by inflammatory leukocytes and some non-leukocytic cells, that act as intercellular mediators. They differ from classical hormones in that they are produced by a number of tissue or cell types rather than by specialized glands. They generally act locally in a paracrine or autocrine rather than endocrine manner.
A soluble factor produced by activated T-LYMPHOCYTES that induces the expression of MHC CLASS II GENES and FC RECEPTORS on B-LYMPHOCYTES and causes their proliferation and differentiation. It also acts on T-lymphocytes, MAST CELLS, and several other hematopoietic lineage cells.
A cytokine produced by a variety of cell types, including T-LYMPHOCYTES; MONOCYTES; DENDRITIC CELLS; and EPITHELIAL CELLS that exerts a variety of effects on immunoregulation and INFLAMMATION. Interleukin-10 combines with itself to form a homodimeric molecule that is the biologically active form of the protein.
Soluble factors which stimulate growth-related activities of leukocytes as well as other cell types. They enhance cell proliferation and differentiation, DNA synthesis, secretion of other biologically active molecules and responses to immune and inflammatory stimuli.
A member of the CXC chemokine family that plays a role in the regulation of the acute inflammatory response. It is secreted by variety of cell types and induces CHEMOTAXIS of NEUTROPHILS and other inflammatory cells.
A ligand that binds to but fails to activate the INTERLEUKIN 1 RECEPTOR. It plays an inhibitory role in the regulation of INFLAMMATION and FEVER. Several isoforms of the protein exist due to multiple ALTERNATIVE SPLICING of its mRNA.
Serum glycoprotein produced by activated MACROPHAGES and other mammalian MONONUCLEAR LEUKOCYTES. It has necrotizing activity against tumor cell lines and increases ability to reject tumor transplants. Also known as TNF-alpha, it is only 30% homologous to TNF-beta (LYMPHOTOXIN), but they share TNF RECEPTORS.
Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others.
Receptors present on activated T-LYMPHOCYTES and B-LYMPHOCYTES that are specific for INTERLEUKIN-2 and play an important role in LYMPHOCYTE ACTIVATION. They are heterotrimeric proteins consisting of the INTERLEUKIN-2 RECEPTOR ALPHA SUBUNIT, the INTERLEUKIN-2 RECEPTOR BETA SUBUNIT, and the INTERLEUKIN RECEPTOR COMMON GAMMA-CHAIN.
The major interferon produced by mitogenically or antigenically stimulated LYMPHOCYTES. It is structurally different from TYPE I INTERFERON and its major activity is immunoregulation. It has been implicated in the expression of CLASS II HISTOCOMPATIBILITY ANTIGENS in cells that do not normally produce them, leading to AUTOIMMUNE DISEASES.
Lymphocytes responsible for cell-mediated immunity. Two types have been identified - cytotoxic (T-LYMPHOCYTES, CYTOTOXIC) and helper T-lymphocytes (T-LYMPHOCYTES, HELPER-INDUCER). They are formed when lymphocytes circulate through the THYMUS GLAND and differentiate to thymocytes. When exposed to an antigen, they divide rapidly and produce large numbers of new T cells sensitized to that antigen.
A heterodimeric cytokine that plays a role in innate and adaptive immune responses. Interleukin-12 is a 70 kDa protein that is composed of covalently linked 40 kDa and 35 kDa subunits. It is produced by DENDRITIC CELLS; MACROPHAGES and a variety of other immune cells and plays a role in the stimulation of INTERFERON-GAMMA production by T-LYMPHOCYTES and NATURAL KILLER CELLS.
Morphologic alteration of small B LYMPHOCYTES or T LYMPHOCYTES in culture into large blast-like cells able to synthesize DNA and RNA and to divide mitotically. It is induced by INTERLEUKINS; MITOGENS such as PHYTOHEMAGGLUTININS, and by specific ANTIGENS. It may also occur in vivo as in GRAFT REJECTION.
RNA sequences that serve as templates for protein synthesis. Bacterial mRNAs are generally primary transcripts in that they do not require post-transcriptional processing. Eukaryotic mRNA is synthesized in the nucleus and must be exported to the cytoplasm for translation. Most eukaryotic mRNAs have a sequence of polyadenylic acid at the 3' end, referred to as the poly(A) tail. The function of this tail is not known for certain, but it may play a role in the export of mature mRNA from the nucleus as well as in helping stabilize some mRNA molecules by retarding their degradation in the cytoplasm.
Proteins prepared by recombinant DNA technology.
Cell surface receptors that are specific for INTERLEUKIN-1. Included under this heading are signaling receptors, non-signaling receptors and accessory proteins required for receptor signaling. Signaling from interleukin-1 receptors occurs via interaction with SIGNAL TRANSDUCING ADAPTOR PROTEINS such as MYELOID DIFFERENTIATION FACTOR 88.
An interleukin-1 subtype that is synthesized as an inactive membrane-bound pro-protein. Proteolytic processing of the precursor form by CASPASE 1 results in release of the active form of interleukin-1beta from the membrane.
A multilineage cell growth factor secreted by LYMPHOCYTES; EPITHELIAL CELLS; and ASTROCYTES which stimulates clonal proliferation and differentiation of various types of blood and tissue cells.
A cytokine that promotes differentiation and activation of EOSINOPHILS. It also triggers activated B-LYMPHOCYTES to differentiate into IMMUNOGLOBULIN-secreting cells.
A cytokine which resembles IL-1 structurally and IL-12 functionally. It enhances the cytotoxic activity of NK CELLS and CYTOTOXIC T-LYMPHOCYTES, and appears to play a role both as neuroimmunomodulator and in the induction of mucosal immunity.
Lipid-containing polysaccharides which are endotoxins and important group-specific antigens. They are often derived from the cell wall of gram-negative bacteria and induce immunoglobulin secretion. The lipopolysaccharide molecule consists of three parts: LIPID A, core polysaccharide, and O-specific chains (O ANTIGENS). When derived from Escherichia coli, lipopolysaccharides serve as polyclonal B-cell mitogens commonly used in laboratory immunology. (From Dorland, 28th ed)
Receptors present on a wide variety of hematopoietic and non-hematopoietic cell types that are specific for INTERLEUKIN-4. They are involved in signaling a variety of immunological responses related to allergic INFLAMMATION including the differentiation of TH2 CELLS and the regulation of IMMUNOGLOBULIN E production. Two subtypes of receptors exist and are referred to as the TYPE I INTERLEUKIN-4 RECEPTOR and the TYPE II INTERLEUKIN-4 RECEPTOR. Each receptor subtype is defined by its unique subunit composition.
A pathological process characterized by injury or destruction of tissues caused by a variety of cytologic and chemical reactions. It is usually manifested by typical signs of pain, heat, redness, swelling, and loss of function.
A cytokine synthesized by T-LYMPHOCYTES that produces proliferation, immunoglobulin isotype switching, and immunoglobulin production by immature B-LYMPHOCYTES. It appears to play a role in regulating inflammatory and immune responses.
Established cell cultures that have the potential to propagate indefinitely.
Cell surface receptors that are specific for INTERLEUKIN-6. They are present on T-LYMPHOCYTES, mitogen-activated B-LYMPHOCYTES, and peripheral MONOCYTES. The receptors are heterodimers of the INTERLEUKIN-6 RECEPTOR ALPHA SUBUNIT and the CYTOKINE RECEPTOR GP130.
A lymphohematopoietic cytokine that plays a role in regulating the proliferation of ERYTHROID PRECURSOR CELLS. It induces maturation of MEGAKARYOCYTES which results in increased production of BLOOD PLATELETS. Interleukin-11 was also initially described as an inhibitor of ADIPOGENESIS of cultured preadipocytes.
Large, phagocytic mononuclear leukocytes produced in the vertebrate BONE MARROW and released into the BLOOD; contain a large, oval or somewhat indented nucleus surrounded by voluminous cytoplasm and numerous organelles.
The fission of a CELL. It includes CYTOKINESIS, when the CYTOPLASM of a cell is divided, and CELL NUCLEUS DIVISION.
Any of the processes by which nuclear, cytoplasmic, or intercellular factors influence the differential control (induction or repression) of gene action at the level of transcription or translation.
The intracellular transfer of information (biological activation/inhibition) through a signal pathway. In each signal transduction system, an activation/inhibition signal from a biologically active molecule (hormone, neurotransmitter) is mediated via the coupling of a receptor/enzyme to a second messenger system or to an ion channel. Signal transduction plays an important role in activating cellular functions, cell differentiation, and cell proliferation. Examples of signal transduction systems are the GAMMA-AMINOBUTYRIC ACID-postsynaptic receptor-calcium ion channel system, the receptor-mediated T-cell activation pathway, and the receptor-mediated activation of phospholipases. Those coupled to membrane depolarization or intracellular release of calcium include the receptor-mediated activation of cytotoxic functions in granulocytes and the synaptic potentiation of protein kinase activation. Some signal transduction pathways may be part of larger signal transduction pathways; for example, protein kinase activation is part of the platelet activation signal pathway.
Cell surface molecules on cells of the immune system that specifically bind surface molecules or messenger molecules and trigger changes in the behavior of cells. Although these receptors were first identified in the immune system, many have important functions elsewhere.
Subset of helper-inducer T-lymphocytes which synthesize and secrete the interleukins IL-4, IL-5, IL-6, and IL-10. These cytokines influence B-cell development and antibody production as well as augmenting humoral responses.
An immunoassay utilizing an antibody labeled with an enzyme marker such as horseradish peroxidase. While either the enzyme or the antibody is bound to an immunosorbent substrate, they both retain their biologic activity; the change in enzyme activity as a result of the enzyme-antibody-antigen reaction is proportional to the concentration of the antigen and can be measured spectrophotometrically or with the naked eye. Many variations of the method have been developed.
A proinflammatory cytokine produced primarily by T-LYMPHOCYTES or their precursors. Several subtypes of interleukin-17 have been identified, each of which is a product of a unique gene.
Technique using an instrument system for making, processing, and displaying one or more measurements on individual cells obtained from a cell suspension. Cells are usually stained with one or more fluorescent dyes specific to cell components of interest, e.g., DNA, and fluorescence of each cell is measured as it rapidly transverses the excitation beam (laser or mercury arc lamp). Fluorescence provides a quantitative measure of various biochemical and biophysical properties of the cell, as well as a basis for cell sorting. Other measurable optical parameters include light absorption and light scattering, the latter being applicable to the measurement of cell size, shape, density, granularity, and stain uptake.
Differentiation antigens residing on mammalian leukocytes. CD stands for cluster of differentiation, which refers to groups of monoclonal antibodies that show similar reactivity with certain subpopulations of antigens of a particular lineage or differentiation stage. The subpopulations of antigens are also known by the same CD designation.
The relatively long-lived phagocytic cell of mammalian tissues that are derived from blood MONOCYTES. Main types are PERITONEAL MACROPHAGES; ALVEOLAR MACROPHAGES; HISTIOCYTES; KUPFFER CELLS of the liver; and OSTEOCLASTS. They may further differentiate within chronic inflammatory lesions to EPITHELIOID CELLS or may fuse to form FOREIGN BODY GIANT CELLS or LANGHANS GIANT CELLS. (from The Dictionary of Cell Biology, Lackie and Dow, 3rd ed.)
An encapsulated lymphatic organ through which venous blood filters.
Cytokine that stimulates the proliferation of T-LYMPHOCYTES and shares biological activities with IL-2. IL-15 also can induce proliferation and differentiation of B-LYMPHOCYTES.
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
A cytokine produced by bone marrow stromal cells that promotes the growth of B-LYMPHOCYTE precursors and is co-mitogenic with INTERLEUKIN-2 for mature T-LYMPHOCYTE activation.
Mature LYMPHOCYTES and MONOCYTES transported by the blood to the body's extravascular space. They are morphologically distinguishable from mature granulocytic leukocytes by their large, non-lobed nuclei and lack of coarse, heavily stained cytoplasmic granules.
A critical subpopulation of T-lymphocytes involved in the induction of most immunological functions. The HIV virus has selective tropism for the T4 cell which expresses the CD4 phenotypic marker, a receptor for HIV. In fact, the key element in the profound immunosuppression seen in HIV infection is the depletion of this subset of T-lymphocytes.
Cells grown in vitro from neoplastic tissue. If they can be established as a TUMOR CELL LINE, they can be propagated in cell culture indefinitely.
The phenotypic manifestation of a gene or genes by the processes of GENETIC TRANSCRIPTION and GENETIC TRANSLATION.
Subset of helper-inducer T-lymphocytes which synthesize and secrete interleukin-2, gamma-interferon, and interleukin-12. Due to their ability to kill antigen-presenting cells and their lymphokine-mediated effector activity, Th1 cells are associated with vigorous delayed-type hypersensitivity reactions.
Bone marrow-derived lymphocytes that possess cytotoxic properties, classically directed against transformed and virus-infected cells. Unlike T CELLS; and B CELLS; NK CELLS are not antigen specific. The cytotoxicity of natural killer cells is determined by the collective signaling of an array of inhibitory and stimulatory CELL SURFACE RECEPTORS. A subset of T-LYMPHOCYTES referred to as NATURAL KILLER T CELLS shares some of the properties of this cell type.
Antibodies produced by a single clone of cells.
Ubiquitous, inducible, nuclear transcriptional activator that binds to enhancer elements in many different cell types and is activated by pathogenic stimuli. The NF-kappa B complex is a heterodimer composed of two DNA-binding subunits: NF-kappa B1 and relA.
An interleukin receptor subunit with specificity for INTERLEUKIN-13. It dimerizes with the INTERLEUKIN-4 RECEPTOR ALPHA SUBUNIT to form the TYPE II INTERLEUKIN-4 RECEPTOR which has specificity for both INTERLEUKIN-4 and INTERLEUKIN-13. Signaling of this receptor subunit occurs through the interaction of its cytoplasmic domain with JANUS KINASES such as the TYK2 KINASE.
The endogenous compounds that mediate inflammation (AUTACOIDS) and related exogenous compounds including the synthetic prostaglandins (PROSTAGLANDINS, SYNTHETIC).
The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.
Cell surface receptors for INTERLEUKIN-13. Included under this heading are the INTERLEUKIN-13 RECEPTOR ALPHA2 which is a monomeric receptor and the INTERLEUKIN-4 RECEPTOR TYPE II which has specificity for both INTERLEUKIN-4 and INTERLEUKIN-13.
Lymphoid cells concerned with humoral immunity. They are short-lived cells resembling bursa-derived lymphocytes of birds in their production of immunoglobulin upon appropriate stimulation.
A variation of the PCR technique in which cDNA is made from RNA via reverse transcription. The resultant cDNA is then amplified using standard PCR protocols.
Soluble protein factors generated by activated lymphocytes that affect other cells, primarily those involved in cellular immunity.
Strains of mice in which certain GENES of their GENOMES have been disrupted, or "knocked-out". To produce knockouts, using RECOMBINANT DNA technology, the normal DNA sequence of the gene being studied is altered to prevent synthesis of a normal gene product. Cloned cells in which this DNA alteration is successful are then injected into mouse EMBRYOS to produce chimeric mice. The chimeric mice are then bred to yield a strain in which all the cells of the mouse contain the disrupted gene. Knockout mice are used as EXPERIMENTAL ANIMAL MODELS for diseases (DISEASE MODELS, ANIMAL) and to clarify the functions of the genes.
Subpopulation of CD4+ lymphocytes that cooperate with other lymphocytes (either T or B) to initiate a variety of immune functions. For example, helper-inducer T-cells cooperate with B-cells to produce antibodies to thymus-dependent antigens and with other subpopulations of T-cells to initiate a variety of cell-mediated immune functions.
The phenomenon of target cell destruction by immunologically active effector cells. It may be brought about directly by sensitized T-lymphocytes or by lymphoid or myeloid "killer" cells, or it may be mediated by cytotoxic antibody, cytotoxic factor released by lymphoid cells, or complement.
Progressive restriction of the developmental potential and increasing specialization of function that leads to the formation of specialized cells, tissues, and organs.
Glycoproteins which contain sialic acid as one of their carbohydrates. They are often found on or in the cell or tissue membranes and participate in a variety of biological activities.
The relationship between the dose of an administered drug and the response of the organism to the drug.
A signal transducer and activator of transcription that mediates cellular responses to INTERLEUKIN-6 family members. STAT3 is constitutively activated in a variety of TUMORS and is a major downstream transducer for the CYTOKINE RECEPTOR GP130.
White blood cells formed in the body's lymphoid tissue. The nucleus is round or ovoid with coarse, irregularly clumped chromatin while the cytoplasm is typically pale blue with azurophilic (if any) granules. Most lymphocytes can be classified as either T or B (with subpopulations of each), or NATURAL KILLER CELLS.
Antigens expressed on the cell membrane of T-lymphocytes during differentiation, activation, and normal and neoplastic transformation. Their phenotypic characterization is important in differential diagnosis and studies of thymic ontogeny and T-cell function.
Cytolytic lymphocytes with the unique capacity of killing natural killer (NK)-resistant fresh tumor cells. They are INTERLEUKIN-2-activated NK cells that have no MAJOR HISTOCOMPATIBILITY COMPLEX restriction or need for antigen stimulation. LAK cells are used for ADOPTIVE IMMUNOTHERAPY in cancer patients.
Signal molecules that are involved in the control of cell growth and differentiation.
Specialized cells of the hematopoietic system that have branch-like extensions. They are found throughout the lymphatic system, and in non-lymphoid tissues such as SKIN and the epithelia of the intestinal, respiratory, and reproductive tracts. They trap and process ANTIGENS, and present them to T-CELLS, thereby stimulating CELL-MEDIATED IMMUNITY. They are different from the non-hematopoietic FOLLICULAR DENDRITIC CELLS, which have a similar morphology and immune system function, but with respect to humoral immunity (ANTIBODY PRODUCTION).
Elements of limited time intervals, contributing to particular results or situations.
Naturally occurring or experimentally induced animal diseases with pathological processes sufficiently similar to those of human diseases. They are used as study models for human diseases.
The uptake of naked or purified DNA by CELLS, usually meaning the process as it occurs in eukaryotic cells. It is analogous to bacterial transformation (TRANSFORMATION, BACTERIAL) and both are routinely employed in GENE TRANSFER TECHNIQUES.
A positive regulatory effect on physiological processes at the molecular, cellular, or systemic level. At the molecular level, the major regulatory sites include membrane receptors, genes (GENE EXPRESSION REGULATION), mRNAs (RNA, MESSENGER), and proteins.
An interleukin-1 subtype that occurs as a membrane-bound pro-protein form that is cleaved by proteases to form a secreted mature form. Unlike INTERLEUKIN-1BETA both membrane-bound and secreted forms of interleukin-1alpha are biologically active.
Complex of at least five membrane-bound polypeptides in mature T-lymphocytes that are non-covalently associated with one another and with the T-cell receptor (RECEPTORS, ANTIGEN, T-CELL). The CD3 complex includes the gamma, delta, epsilon, zeta, and eta chains (subunits). When antigen binds to the T-cell receptor, the CD3 complex transduces the activating signals to the cytoplasm of the T-cell. The CD3 gamma and delta chains (subunits) are separate from and not related to the gamma/delta chains of the T-cell receptor (RECEPTORS, ANTIGEN, T-CELL, GAMMA-DELTA).
The rate dynamics in chemical or physical systems.
Mucoproteins isolated from the kidney bean (Phaseolus vulgaris); some of them are mitogenic to lymphocytes, others agglutinate all or certain types of erythrocytes or lymphocytes. They are used mainly in the study of immune mechanisms and in cell culture.
A heterodimeric cytokine that plays a role in innate and adaptive immune responses. Interleukin-23 is comprised of a unique 19 kDa subunit and 40 kDa subunit that is shared with INTERLEUKIN-12. It is produced by DENDRITIC CELLS; MACROPHAGES and a variety of other immune cells
An interleukin-1 receptor subtype that is involved in signaling cellular responses to INTERLEUKIN-1ALPHA and INTERLEUKIN-1BETA. The binding of this receptor to its ligand causes its favorable interaction with INTERLEUKIN-1 RECEPTOR ACCESSORY PROTEIN and the formation of an activated receptor complex.
Measurable and quantifiable biological parameters (e.g., specific enzyme concentration, specific hormone concentration, specific gene phenotype distribution in a population, presence of biological substances) which serve as indices for health- and physiology-related assessments, such as disease risk, psychiatric disorders, environmental exposure and its effects, disease diagnosis, metabolic processes, substance abuse, pregnancy, cell line development, epidemiologic studies, etc.
Genetically identical individuals developed from brother and sister matings which have been carried out for twenty or more generations, or by parent x offspring matings carried out with certain restrictions. All animals within an inbred strain trace back to a common ancestor in the twentieth generation.
A cytokine receptor that acts through the formation of oligomeric complexes of itself with a variety of CYTOKINE RECEPTORS.
Cell surface receptors that are specific for INTERLEUKIN-5. They are heterodimeric proteins consisting of the INTERLEUKIN-5 RECEPTOR ALPHA SUBUNIT and the CYTOKINE RECEPTOR COMMON BETA SUBUNIT. Signaling from interleukin-5 receptors can occur through interaction of their cytoplasmic domains with SYNTENINS.
A long pro-domain caspase that has specificity for the precursor form of INTERLEUKIN-1BETA. It plays a role in INFLAMMATION by catalytically converting the inactive forms of CYTOKINES such as interleukin-1beta to their active, secreted form. Caspase 1 is referred as interleukin-1beta converting enzyme and is frequently abbreviated ICE.
The biosynthesis of RNA carried out on a template of DNA. The biosynthesis of DNA from an RNA template is called REVERSE TRANSCRIPTION.
A signal transducer and activator of transcription that mediates cellular responses to INTERLEUKIN-4. Stat6 has been shown to partner with NF-KAPPA B and CCAAT-ENHANCER-BINDING PROTEINS to regulate GENETIC TRANSCRIPTION of interleukin-4 responsive GENES.
Glycoproteins found on the membrane or surface of cells.
The most common and most biologically active of the mammalian prostaglandins. It exhibits most biological activities characteristic of prostaglandins and has been used extensively as an oxytocic agent. The compound also displays a protective effect on the intestinal mucosa.
An immunoglobulin associated with MAST CELLS. Overexpression has been associated with allergic hypersensitivity (HYPERSENSITIVITY, IMMEDIATE).
Cell surface receptors for INTERLEUKIN-10. They exist as a tetramer of two alpha chains (INTERLEUKIN-10 RECEPTOR ALPHA CHAIN) and two beta chains (INTERLEUKIN-10 RECEPTOR, BETA CHAIN). Signaling from interleukin-10 receptors occurs through their interaction with JANUS KINASES.
Manipulation of the host's immune system in treatment of disease. It includes both active and passive immunization as well as immunosuppressive therapy to prevent graft rejection.
Cell surface receptors that are specific for INTERLEUKIN-7. They are present on T-LYMPHOCYTES and B-LYMPHOCYTE precursors. The receptors are heterodimeric proteins consisting of the INTERLEUKIN-5 RECEPTOR ALPHA SUBUNIT and the CYTOKINE RECEPTOR COMMON BETA SUBUNIT.
High affinity receptors for INTERLEUKIN-3. They are found on early HEMATOPOIETIC PROGENITOR CELLS; progenitors of MYELOID CELLS; EOSINOPHILS; and BASOPHILS. Interleukin-3 receptors are formed by the dimerization of the INTERLEUKIN-3 RECEPTOR ALPHA SUBUNIT and the CYTOKINE RECEPTOR COMMON BETA SUBUNIT.
A cytokine subunit that is a component of both interleukin-12 and interleukin-23. It binds to the INTERLEUKIN-12 SUBUNIT P35 via a disulfide bond to form interleukin-12 and to INTERLEUKIN-23 SUBUNIT P19 to form interleukin-23.
Molecules on the surface of T-lymphocytes that recognize and combine with antigens. The receptors are non-covalently associated with a complex of several polypeptides collectively called CD3 antigens (ANTIGENS, CD3). Recognition of foreign antigen and the major histocompatibility complex is accomplished by a single heterodimeric antigen-receptor structure, composed of either alpha-beta (RECEPTORS, ANTIGEN, T-CELL, ALPHA-BETA) or gamma-delta (RECEPTORS, ANTIGEN, T-CELL, GAMMA-DELTA) chains.
Either of the pair of organs occupying the cavity of the thorax that effect the aeration of the blood.
A classification of T-lymphocytes, especially into helper/inducer, suppressor/effector, and cytotoxic subsets, based on structurally or functionally different populations of cells.
Cells contained in the bone marrow including fat cells (see ADIPOCYTES); STROMAL CELLS; MEGAKARYOCYTES; and the immediate precursors of most blood cells.
Granular leukocytes with a nucleus that usually has two lobes connected by a slender thread of chromatin, and cytoplasm containing coarse, round granules that are uniform in size and stainable by eosin.
The action of a drug in promoting or enhancing the effectiveness of another drug.
A subunit of the interleukin-18 receptor that is responsible of extracellular binding of IL-18.
Progenitor cells from which all blood cells derive.
Granular leukocytes having a nucleus with three to five lobes connected by slender threads of chromatin, and cytoplasm containing fine inconspicuous granules and stainable by neutral dyes.
High-affinity G-protein-coupled receptors for INTERLEUKIN-8 present on NEUTROPHILS; MONOCYTES; and BASOPHILS.
A critical subpopulation of regulatory T-lymphocytes involved in MHC Class I-restricted interactions. They include both cytotoxic T-lymphocytes (T-LYMPHOCYTES, CYTOTOXIC) and CD8+ suppressor T-lymphocytes.
In vitro method for producing large amounts of specific DNA or RNA fragments of defined length and sequence from small amounts of short oligonucleotide flanking sequences (primers). The essential steps include thermal denaturation of the double-stranded target molecules, annealing of the primers to their complementary sequences, and extension of the annealed primers by enzymatic synthesis with DNA polymerase. The reaction is efficient, specific, and extremely sensitive. Uses for the reaction include disease diagnosis, detection of difficult-to-isolate pathogens, mutation analysis, genetic testing, DNA sequencing, and analyzing evolutionary relationships.
Antigens on surfaces of cells, including infectious or foreign cells or viruses. They are usually protein-containing groups on cell membranes or walls and may be isolated.
Cell surface receptors for INTERLEUKIN-18 found on a variety of cell types including MACROPHAGES; NEUTROPHILS; NK CELLS; ENDOTHELIAL CELLS; and SMOOTH MUSCLE CELLS. They are formed as a heterodimer of alpha and beta subunits.
DNA sequences which are recognized (directly or indirectly) and bound by a DNA-dependent RNA polymerase during the initiation of transcription. Highly conserved sequences within the promoter include the Pribnow box in bacteria and the TATA BOX in eukaryotes.
Class of pro-inflammatory cytokines that have the ability to attract and activate leukocytes. They can be divided into at least three structural branches: C; (CHEMOKINES, C); CC; (CHEMOKINES, CC); and CXC; (CHEMOKINES, CXC); according to variations in a shared cysteine motif.
One of the mechanisms by which CELL DEATH occurs (compare with NECROSIS and AUTOPHAGOCYTOSIS). Apoptosis is the mechanism responsible for the physiological deletion of cells and appears to be intrinsically programmed. It is characterized by distinctive morphologic changes in the nucleus and cytoplasm, chromatin cleavage at regularly spaced sites, and the endonucleolytic cleavage of genomic DNA; (DNA FRAGMENTATION); at internucleosomal sites. This mode of cell death serves as a balance to mitosis in regulating the size of animal tissues and in mediating pathologic processes associated with tumor growth.
The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.
Connective tissue cells which secrete an extracellular matrix rich in collagen and other macromolecules.
The introduction of a phosphoryl group into a compound through the formation of an ester bond between the compound and a phosphorus moiety.
Substances that reduce or suppress INFLAMMATION.
A phorbol ester found in CROTON OIL with very effective tumor promoting activity. It stimulates the synthesis of both DNA and RNA.
Laboratory mice that have been produced from a genetically manipulated EGG or EMBRYO, MAMMALIAN.
A MANNOSE/GLUCOSE binding lectin isolated from the jack bean (Canavalia ensiformis). It is a potent mitogen used to stimulate cell proliferation in lymphocytes, primarily T-lymphocyte, cultures.
Manifestations of the immune response which are mediated by antigen-sensitized T-lymphocytes via lymphokines or direct cytotoxicity. This takes place in the absence of circulating antibody or where antibody plays a subordinate role.
A chemokine that is a chemoattractant for MONOCYTES and may also cause cellular activation of specific functions related to host defense. It is produced by LEUKOCYTES of both monocyte and lymphocyte lineage and by FIBROBLASTS during tissue injury. It has specificity for CCR2 RECEPTORS.
Washing liquid obtained from irrigation of the lung, including the BRONCHI and the PULMONARY ALVEOLI. It is generally used to assess biochemical, inflammatory, or infection status of the lung.
Proteins which bind to DNA. The family includes proteins which bind to both double- and single-stranded DNA and also includes specific DNA binding proteins in serum which can be used as markers for malignant diseases.
A multifunctional cytokine secreted by primarily by activated TH2 CELLS that may play a role as a regulator of allergic INFLAMMATION. It has been shown to enhance the growth and CELL DIFFERENTIATION of MAST CELLS, and can act on a variety of other immune cells.
Cell surface receptors for INTERLEUKIN-12. They exist as dimers of beta 1 and beta 2 subunits. Signaling from interleukin-12 receptors occurs through their interaction with JANUS KINASES.
Soluble mediators of the immune response that are neither antibodies nor complement. They are produced largely, but not exclusively, by monocytes and macrophages.
Histochemical localization of immunoreactive substances using labeled antibodies as reagents.
Short sequences (generally about 10 base pairs) of DNA that are complementary to sequences of messenger RNA and allow reverse transcriptases to start copying the adjacent sequences of mRNA. Primers are used extensively in genetic and molecular biology techniques.
Subset of helper-effector T-lymphocytes which synthesize and secrete IL-17, IL-17F, and IL-22. These cytokines are involved in host defenses and tissue inflammation in autoimmune diseases.
A negative regulatory effect on physiological processes at the molecular, cellular, or systemic level. At the molecular level, the major regulatory sites include membrane receptors, genes (GENE EXPRESSION REGULATION), mRNAs (RNA, MESSENGER), and proteins.
Glycoproteins found in a subfraction of normal mammalian plasma and urine. They stimulate the proliferation of bone marrow cells in agar cultures and the formation of colonies of granulocytes and/or macrophages. The factors include INTERLEUKIN-3; (IL-3); GRANULOCYTE COLONY-STIMULATING FACTOR; (G-CSF); MACROPHAGE COLONY-STIMULATING FACTOR; (M-CSF); and GRANULOCYTE-MACROPHAGE COLONY-STIMULATING FACTOR; (GM-CSF).
Cell surface receptors for INTERLEUKIN-15. They are widely-distributed heterotrimeric proteins consisting of the INTERLEUKIN-15 RECEPTOR ALPHA SUBUNIT, the INTERLEUKIN-2, 15 RECEPTOR BETA SUBUNIT, and the INTERLEUKIN RECEPTOR COMMON GAMMA-CHAIN.
The capacity of a normal organism to remain unaffected by microorganisms and their toxins. It results from the presence of naturally occurring ANTI-INFECTIVE AGENTS, constitutional factors such as BODY TEMPERATURE and immediate acting immune cells such as NATURAL KILLER CELLS.
The process of altering the morphology and functional activity of macrophages so that they become avidly phagocytic. It is initiated by lymphokines, such as the macrophage activation factor (MAF) and the macrophage migration-inhibitory factor (MMIF), immune complexes, C3b, and various peptides, polysaccharides, and immunologic adjuvants.
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
Immunoglobulin molecules having a specific amino acid sequence by virtue of which they interact only with the ANTIGEN (or a very similar shape) that induced their synthesis in cells of the lymphoid series (especially PLASMA CELLS).
A plasma protein that circulates in increased amounts during inflammation and after tissue damage.
Cell surface receptors for INTERLEUKIN-17. Several subtypes of receptors have been found, each with its own in specificity for interleukin-17 subtype.
A malignant neoplasm derived from cells that are capable of forming melanin, which may occur in the skin of any part of the body, in the eye, or, rarely, in the mucous membranes of the genitalia, anus, oral cavity, or other sites. It occurs mostly in adults and may originate de novo or from a pigmented nevus or malignant lentigo. Melanomas frequently metastasize widely, and the regional lymph nodes, liver, lungs, and brain are likely to be involved. The incidence of malignant skin melanomas is rising rapidly in all parts of the world. (Stedman, 25th ed; from Rook et al., Textbook of Dermatology, 4th ed, p2445)
Inflammation of the COLON section of the large intestine (INTESTINE, LARGE), usually with symptoms such as DIARRHEA (often with blood and mucus), ABDOMINAL PAIN, and FEVER.
The inner membrane of a joint capsule surrounding a freely movable joint. It is loosely attached to the external fibrous capsule and secretes SYNOVIAL FLUID.
The span of viability of a cell characterized by the capacity to perform certain functions such as metabolism, growth, reproduction, some form of responsiveness, and adaptability.
A group of genetically identical cells all descended from a single common ancestral cell by mitosis in eukaryotes or by binary fission in prokaryotes. Clone cells also include populations of recombinant DNA molecules all carrying the same inserted sequence. (From King & Stansfield, Dictionary of Genetics, 4th ed)
The outward appearance of the individual. It is the product of interactions between genes, and between the GENOTYPE and the environment.
An anti-inflammatory 9-fluoro-glucocorticoid.
Studies which start with the identification of persons with a disease of interest and a control (comparison, referent) group without the disease. The relationship of an attribute to the disease is examined by comparing diseased and non-diseased persons with regard to the frequency or levels of the attribute in each group.
A chronic systemic disease, primarily of the joints, marked by inflammatory changes in the synovial membranes and articular structures, widespread fibrinoid degeneration of the collagen fibers in mesenchymal tissues, and by atrophy and rarefaction of bony structures. Etiology is unknown, but autoimmune mechanisms have been implicated.
A deoxyribonucleotide polymer that is the primary genetic material of all cells. Eukaryotic and prokaryotic organisms normally contain DNA in a double-stranded state, yet several important biological processes transiently involve single-stranded regions. DNA, which consists of a polysugar-phosphate backbone possessing projections of purines (adenine and guanine) and pyrimidines (thymine and cytosine), forms a double helix that is held together by hydrogen bonds between these purines and pyrimidines (adenine to thymine and guanine to cytosine).
Immunized T-lymphocytes which can directly destroy appropriate target cells. These cytotoxic lymphocytes may be generated in vitro in mixed lymphocyte cultures (MLC), in vivo during a graft-versus-host (GVH) reaction, or after immunization with an allograft, tumor cell or virally transformed or chemically modified target cell. The lytic phenomenon is sometimes referred to as cell-mediated lympholysis (CML). These CD8-positive cells are distinct from NATURAL KILLER CELLS and NATURAL KILLER T-CELLS. There are two effector phenotypes: TC1 and TC2.
A low affinity interleukin-11 receptor subunit that combines with the CYTOKINE RECEPTOR GP130 to form a high affinity receptor for INTERLEUKIN-11. Multiple isoforms of this protein exist due to ALTERNATIVE SPLICING of its MRNA.
A factor synthesized in a wide variety of tissues. It acts synergistically with TGF-alpha in inducing phenotypic transformation and can also act as a negative autocrine growth factor. TGF-beta has a potential role in embryonal development, cellular differentiation, hormone secretion, and immune function. TGF-beta is found mostly as homodimer forms of separate gene products TGF-beta1, TGF-beta2 or TGF-beta3. Heterodimers composed of TGF-beta1 and 2 (TGF-beta1.2) or of TGF-beta2 and 3 (TGF-beta2.3) have been isolated. The TGF-beta proteins are synthesized as precursor proteins.
The specific failure of a normally responsive individual to make an immune response to a known antigen. It results from previous contact with the antigen by an immunologically immature individual (fetus or neonate) or by an adult exposed to extreme high-dose or low-dose antigen, or by exposure to radiation, antimetabolites, antilymphocytic serum, etc.
A cytokine produced by activated T-LYMPHOCYTES that stimulates the migration of CD4-POSITIVE LYMPHOCYTES and monocytes. It has been reported to suppress HIV replication.
Detection of RNA that has been electrophoretically separated and immobilized by blotting on nitrocellulose or other type of paper or nylon membrane followed by hybridization with labeled NUCLEIC ACID PROBES.
The number of WHITE BLOOD CELLS per unit volume in venous BLOOD. A differential leukocyte count measures the relative numbers of the different types of white cells.
Substances that augment, stimulate, activate, potentiate, or modulate the immune response at either the cellular or humoral level. The classical agents (Freund's adjuvant, BCG, Corynebacterium parvum, et al.) contain bacterial antigens. Some are endogenous (e.g., histamine, interferon, transfer factor, tuftsin, interleukin-1). Their mode of action is either non-specific, resulting in increased immune responsiveness to a wide variety of antigens, or antigen-specific, i.e., affecting a restricted type of immune response to a narrow group of antigens. The therapeutic efficacy of many biological response modifiers is related to their antigen-specific immunoadjuvanticity.
Process of classifying cells of the immune system based on structural and functional differences. The process is commonly used to analyze and sort T-lymphocytes into subsets based on CD antigens by the technique of flow cytometry.
An early local inflammatory reaction to insult or injury that consists of fever, an increase in inflammatory humoral factors, and an increased synthesis by hepatocytes of a number of proteins or glycoproteins usually found in the plasma.
An INTERLEUKIN-6 related cytokine that exhibits pleiotrophic effects on many physiological systems that involve cell proliferation, differentiation, and survival. Leukemia inhibitory factor binds to and acts through the lif receptor.
A technique of culturing mixed cell types in vitro to allow their synergistic or antagonistic interactions, such as on CELL DIFFERENTIATION or APOPTOSIS. Coculture can be of different types of cells, tissues, or organs from normal or disease states.
A cell-surface ligand involved in leukocyte adhesion and inflammation. Its production is induced by gamma-interferon and it is required for neutrophil migration into inflamed tissue.
Cells that line the inner and outer surfaces of the body by forming cellular layers (EPITHELIUM) or masses. Epithelial cells lining the SKIN; the MOUTH; the NOSE; and the ANAL CANAL derive from ectoderm; those lining the RESPIRATORY SYSTEM and the DIGESTIVE SYSTEM derive from endoderm; others (CARDIOVASCULAR SYSTEM and LYMPHATIC SYSTEM) derive from mesoderm. Epithelial cells can be classified mainly by cell shape and function into squamous, glandular and transitional epithelial cells.
Diffusible gene products that act on homologous or heterologous molecules of viral or cellular DNA to regulate the expression of proteins.
Protein kinases that catalyze the PHOSPHORYLATION of TYROSINE residues in proteins with ATP or other nucleotides as phosphate donors.
Lining of the INTESTINES, consisting of an inner EPITHELIUM, a middle LAMINA PROPRIA, and an outer MUSCULARIS MUCOSAE. In the SMALL INTESTINE, the mucosa is characterized by a series of folds and abundance of absorptive cells (ENTEROCYTES) with MICROVILLI.
Recombinant proteins produced by the GENETIC TRANSLATION of fused genes formed by the combination of NUCLEIC ACID REGULATORY SEQUENCES of one or more genes with the protein coding sequences of one or more genes.
An interleukin receptor subunit that was originally discovered as a component of the INTERLEUKIN 2 RECEPTOR. It was subsequently found to be a component of several other receptors including the INTERLEUKIN 4 RECEPTOR, the INTERLEUKIN 7 RECEPTOR, the INTERLEUKIN-9 RECEPTOR, the INTERLEUKIN-15 RECEPTOR, and the INTERLEUKIN-21 RECEPTOR. Mutations in the gene for the interleukin receptor common gamma chain have been associated with X-LINKED COMBINED IMMUNODEFICIENCY DISEASES.
The major immunoglobulin isotype class in normal human serum. There are several isotype subclasses of IgG, for example, IgG1, IgG2A, and IgG2B.
Mononuclear phagocytes derived from bone marrow precursors but resident in the peritoneum.
A pattern recognition receptor that interacts with LYMPHOCYTE ANTIGEN 96 and LIPOPOLYSACCHARIDES. It mediates cellular responses to GRAM-NEGATIVE BACTERIA.
All of the processes involved in increasing CELL NUMBER including CELL DIVISION.
An albumin obtained from the white of eggs. It is a member of the serpin superfamily.
They are oval or bean shaped bodies (1 - 30 mm in diameter) located along the lymphatic system.
Toxins closely associated with the living cytoplasm or cell wall of certain microorganisms, which do not readily diffuse into the culture medium, but are released upon lysis of the cells.
Glycoprotein molecules on the surface of B- and T-lymphocytes, that react with molecules of antilymphocyte sera, lectins, and other agents which induce blast transformation of lymphocytes.
Granulated cells that are found in almost all tissues, most abundantly in the skin and the gastrointestinal tract. Like the BASOPHILS, mast cells contain large amounts of HISTAMINE and HEPARIN. Unlike basophils, mast cells normally remain in the tissues and do not circulate in the blood. Mast cells, derived from the bone marrow stem cells, are regulated by the STEM CELL FACTOR.
CD4-positive T cells that inhibit immunopathology or autoimmune disease in vivo. They inhibit the immune response by influencing the activity of other cell types. Regulatory T-cells include naturally occurring CD4+CD25+ cells, IL-10 secreting Tr1 cells, and Th3 cells.
A Janus kinase subtype that is predominantly expressed in hematopoietic cell. It is involved in signaling from a broad variety of CYTOKINE RECEPTORS including ones that utilize the INTERLEUKIN RECEPTOR COMMON GAMMA SUBUNIT.
Conversion of an inactive form of an enzyme to one possessing metabolic activity. It includes 1, activation by ions (activators); 2, activation by cofactors (coenzymes); and 3, conversion of an enzyme precursor (proenzyme or zymogen) to an active enzyme.
A cell surface receptor that specifically mediates the biological effects of INTERLEUKIN-9. The functional IL9 receptor signals through interaction of its cytoplasm domain with JANUS KINASES and requires the INTERLEUKIN RECEPTOR COMMON GAMMA SUBUNIT for activity.
Proteins that are secreted into the blood in increased or decreased quantities by hepatocytes in response to trauma, inflammation, or disease. These proteins can serve as inhibitors or mediators of the inflammatory processes. Certain acute-phase proteins have been used to diagnose and follow the course of diseases or as tumor markers.
A classification of lymphocytes based on structurally or functionally different populations of cells.
An interleukin-1 receptor subtype that competes with the INTERLEUKIN-1 RECEPTOR TYPE I for binding to INTERLEUKIN-1ALPHA and INTERLEUKIN-1BETA. The interleukin-1 type II receptor appears to lack signal transduction capability. Therefore it may act as a "decoy" receptor that modulates the activity of its ligands. Both membrane-bound and soluble forms of the receptor have been identified.
White blood cells. These include granular leukocytes (BASOPHILS; EOSINOPHILS; and NEUTROPHILS) as well as non-granular leukocytes (LYMPHOCYTES and MONOCYTES).
Biologically active substances whose activities affect or play a role in the functioning of the immune system.
Differentiation antigens found on thymocytes and on cytotoxic and suppressor T-lymphocytes. CD8 antigens are members of the immunoglobulin supergene family and are associative recognition elements in MHC (Major Histocompatibility Complex) Class I-restricted interactions.
A cytokine with both pro- and anti-inflammatory actions that depend upon the cellular microenvironment. Oncostatin M is a 28 kDa monomeric glycoprotein that is similar in structure to LEUKEMIA INHIBITORY FACTOR. Its name derives from the the observation that it inhibited the growth of tumor cells and augmented the growth of normal fibroblasts.
A family of pattern recognition receptors characterized by an extracellular leucine-rich domain and a cytoplasmic domain that share homology with the INTERLEUKIN 1 RECEPTOR and the DROSOPHILA toll protein. Following pathogen recognition, toll-like receptors recruit and activate a variety of SIGNAL TRANSDUCING ADAPTOR PROTEINS.
Culture media containing biologically active components obtained from previously cultured cells or tissues that have released into the media substances affecting certain cell functions (e.g., growth, lysis).
Mice homozygous for the mutant autosomal recessive gene "scid" which is located on the centromeric end of chromosome 16. These mice lack mature, functional lymphocytes and are thus highly susceptible to lethal opportunistic infections if not chronically treated with antibiotics. The lack of B- and T-cell immunity resembles severe combined immunodeficiency (SCID) syndrome in human infants. SCID mice are useful as animal models since they are receptive to implantation of a human immune system producing SCID-human (SCID-hu) hematochimeric mice.
The soft tissue filling the cavities of bones. Bone marrow exists in two types, yellow and red. Yellow marrow is found in the large cavities of large bones and consists mostly of fat cells and a few primitive blood cells. Red marrow is a hematopoietic tissue and is the site of production of erythrocytes and granular leukocytes. Bone marrow is made up of a framework of connective tissue containing branching fibers with the frame being filled with marrow cells.
Cell surface receptors that bind TUMOR NECROSIS FACTORS and trigger changes which influence the behavior of cells.
A single, unpaired primary lymphoid organ situated in the MEDIASTINUM, extending superiorly into the neck to the lower edge of the THYROID GLAND and inferiorly to the fourth costal cartilage. It is necessary for normal development of immunologic function early in life. By puberty, it begins to involute and much of the tissue is replaced by fat.
A specific immune response elicited by a specific dose of an immunologically active substance or cell in an organism, tissue, or cell.
A large lobed glandular organ in the abdomen of vertebrates that is responsible for detoxification, metabolism, synthesis and storage of various substances.

Prevention of collagen-induced arthritis by gene delivery of soluble p75 tumour necrosis factor receptor. (1/7363)

Collagen type II-induced arthritis (CIA) in DBA/1 mice can be passively transferred to SCID mice with spleen B- and T-lymphocytes. In the present study, we show that infection ex vivo of splenocytes from arthritic DBA/1 mice with a retroviral vector, containing cDNA for the soluble form of human p75 receptor of tumour necrosis factor (TNF-R) before transfer, prevents the development of arthritis, bone erosion and joint inflammation in the SCID recipients. Assessment of IgG subclass levels and studies of synovial histology suggest that down-regulating the effector functions of T helper-type 1 (Th1) cells may, at least in part, explain the inhibition of arthritis in the SCID recipients. In contrast, the transfer of splenocytes infected with mouse TNF-alpha gene construct resulted in exacerbated arthritis and enhancement of IgG2a antibody levels. Intriguingly, infection of splenocytes from arthritic DBA/1 mice with a construct for mouse IL-10 had no modulating effect on the transfer of arthritis. The data suggest that manipulation of the immune system with cytokines, or cytokine inhibitors using gene transfer protocols can be an effective approach to ameliorate arthritis.  (+info)

Enhanced Th1 activity and development of chronic enterocolitis in mice devoid of Stat3 in macrophages and neutrophils. (2/7363)

We have generated mice with a cell type-specific disruption of the Stat3 gene in macrophages and neutrophils. The mutant mice are highly susceptible to endotoxin shock with increased production of inflammatory cytokines such as TNF alpha, IL-1, IFN gamma, and IL-6. Endotoxin-induced production of inflammatory cytokines is augmented because the suppressive effects of IL-10 on inflammatory cytokine production from macrophages and neutrophils are completely abolished. The mice show a polarized immune response toward the Th1 type and develop chronic enterocolitis with age. Taken together, Stat3 plays a critical role in deactivation of macrophages and neutrophils mainly exerted by IL-10.  (+info)

Estrogen enhancement of anti-double-stranded DNA antibody and immunoglobulin G production in peripheral blood mononuclear cells from patients with systemic lupus erythematosus. (3/7363)

OBJECTIVE: To study the in vitro effect of estrogen on IgG anti-double-stranded DNA (anti-dsDNA) antibody and total IgG production in peripheral blood mononuclear cells (PBMC) from patients with systemic lupus erythematosus (SLE), in order to elucidate its regulatory role in SLE. METHODS: PBMC from SLE patients and normal donors were cultured with 17beta-estradiol (E2). IgG anti-dsDNA antibodies, total IgG, and cytokine activity in the culture supernatants were measured by enzyme-linked immunosorbent assay. RESULTS: E2 enhanced production of IgG anti-dsDNA antibodies as well as total IgG in PBMC from SLE patients. Anti-dsDNA production in patients with inactive disease was less responsive to E2 than that in patients with active disease. E2 also enhanced total IgG, but not anti-dsDNA, production in the PBMC of normal donors. Antibody production was increased by E2 to a lesser extent in patients' B cells than in their PBMC. Anti-interleukin-10 (anti-IL-10) antibodies partially blocked the E2-induced increase in antibody production in patients' PBMC, but anti-IL-10 had no effect on B cells. E2 increased IL-10 production by patients' monocytes. Exogenous IL-10 acted additively with E2 in increasing antibody production in patients' B cells. CONCLUSION: These results suggest that E2 may polyclonally increase the production of IgG, including IgG anti-dsDNA, in SLE patients' PBMC by enhancing B cell activity and by promoting IL-10 production in monocytes. These findings support the involvement of E2 in the pathogenesis of SLE.  (+info)

CD40 signaling of monocyte inflammatory cytokine synthesis through an ERK1/2-dependent pathway. A target of interleukin (il)-4 and il-10 anti-inflammatory action. (4/7363)

Ligation of CD40 on monocytes through its interaction with CD40 ligand (CD154) present on activated T helper cells, results in activation of monocyte inflammatory cytokine synthesis and rescue of monocytes from apoptosis induced through serum deprivation. Both of these consequences of CD40 stimulation have been shown to be dependent on the induction of protein tyrosine kinase activity. CD40-mediated activation of protein tyrosine kinase activity and subsequent inflammatory cytokine production are abrogated by treatment of monocytes with the T helper type 2 cytokines interleukin 4 (IL-4) and interleukin 10 (IL-10). In the current study we demonstrate that stimulation of monocytes through CD40 resulted in the phosphorylation and activation of the extracellular signal-regulated kinases 1 and 2 (ERK1/2) mitogen-activated protein kinases, whereas phosphorylation of mitogen-activated protein kinases family members p38 and c-Jun N-terminal kinase was not observed in response to this stimuli over the time course examined. PD98059, an inhibitor of the upstream activator of ERK1/2, the MAP/ERK kinase MEK1/2, suppressed IL-1beta and tumor necrosis factor-alpha production in a dose-dependent fashion. Pretreatment of monocytes with IL-4 and IL-10 inhibited CD40-mediated activation of ERK1/2 kinase activity when used individually, and are enhanced in effectiveness when used in combination. Together, the data demonstrate that CD40-mediated induction of IL-1beta and tumor necrosis factor-alpha synthesis is dependent on a MEK/ERK pathway which is obstructed by signals generated through the action of IL-4 and IL-10.  (+info)

Tumor-induced interleukin-10 inhibits type 1 immune responses directed at a tumor antigen as well as a non-tumor antigen present at the tumor site. (5/7363)

Interleukin (IL)-10 is a potent immunosuppressive cytokine that has been found to be present at the tumor site in a wide variety of human cancers, including transitional cell carcinoma of the bladder. Using a murine bladder tumor (MB49), which we show to express the male transplantation antigen (HY), we tested the hypothesis that IL-10 at the tumor site can block the generation of a tumor-specific type 1 immune response. We show that, despite its expression of HY, MB49 fails to prime for an HY-specific type 1 (IFN-gamma) response in normal female mice. Although MB49 does not constitutively produce IL-10, our data support a model whereby MB49 induces infiltrating cells to produce IL-10. This feature rendered the IL-10 knockout (KO) mouse, whose infiltrating cells are incapable of IL-10 production, a suitable model in which to study MB49 in the absence of IL-10. When injected into IL-10 KO mice, MB49 does prime for an HY-specific, type 1 immune response. Furthermore, IL-10 KO mice show prolonged survival and an increased capacity to reject tumors as compared with normal mice. We also tested the ability of tumor-induced IL-10 to inhibit immunization to a non-tumor antigen present at the tumor site. When vaccinia virus encoding beta-galactosidase (beta-gal) is injected into the tumors of normal mice, no beta-gal-specific IFN-gamma response is mounted. However, when this same viral construct is injected into the tumors of IL-10 KO mice, it produces a strong beta-gal-specific, IFN-gamma response. These studies demonstrate that tumor-induced IL-10 can block the generation of a tumor-specific type 1 immune response as well as subvert attempts to elicit a type 1 immune response to a non-tumor antigen at the tumor site.  (+info)

Interleukin-10 inhibits expression of both interferon alpha- and interferon gamma- induced genes by suppressing tyrosine phosphorylation of STAT1. (6/7363)

Interleukin-10 (IL-10) helps maintain polarized T-helper cells in a T-helper lymphocyte 2 (Th2) phenotype. Part of this process involves the prevention of the development of Th1 cells, which are a primary source of interferon gamma (IFNgamma), a potent activator of monocytes and an inhibitor of Th2 proliferation. Because monocytes and macrophages are important mediators of Th1-type responses, such as delayed-type hypersensitivity, we sought to determine if IL-10 could directly mediate inhibition of IFNgamma- and IFNalpha-induced gene expression in these cells. Highly purified monocytes were incubated with IL-10 for 60 to 90 minutes before the addition of IFNgamma or IFNalpha. IL-10 preincubation resulted in the inhibition of gene expression for several IFN-induced genes, such as IP-10, ISG54, and intercellular adhesion molecule-1. The reduction in gene expression resulted from the ability of IL-10 to suppress IFN-induced assembly of signal transducer and activator of transcription (STAT) factors to specific promoter motifs on IFNalpha- and IFNgamma-inducible genes. This was accomplished by preventing the IFN-induced tyrosine phosphorylation of STAT1, a component of both IFNalpha- and IFNgamma-induced DNA binding complexes. Therefore, IL-10 can directly inhibit STAT-dependent early response gene expression induced by both IFNalpha and IFNgamma in monocytes by suppressing the tyrosine phosphorylation of STAT1. This may occur through the ability of IL-10 to induce expression of the gene, suppressor of cytokine signaling 3 (SOCS3).  (+info)

Interleukin-10-treated human dendritic cells induce a melanoma-antigen-specific anergy in CD8(+) T cells resulting in a failure to lyse tumor cells. (7/7363)

Dendritic cells (DC) are critically involved in the initiation of primary immune processes, including tumor rejection. In our study, we investigated the effect of interleukin-10 (IL-10)-treated human DC on the properties of CD8(+) T cells that are known to be essential for the destruction of tumor cells. We show that IL-10-pretreatment of DC not only reduces their allostimulatory capacity, but also induces a state of alloantigen-specific anergy in both primed and naive (CD45RA+) CD8(+) T cells. To investigate the influence of IL-10-treated DC on melanoma-associated antigen-specific T cells, we generated a tyrosinase-specific CD8(+) T-cell line by several rounds of stimulation with the specific antigen. After coculture with IL-10-treated DC, restimulation of the T-cell line with untreated, antigen-pulsed DC demonstrated peptide-specific anergy in the tyrosinase-specific T cells. Addition of IL-2 to the anergic T cells reversed the state of both alloantigen- or peptide-specific anergy. In contrast to optimally stimulated CD8(+) T cells, anergic tyrosinase-specific CD8(+) T cells, after coculture with peptide-pulsed IL-10-treated DC, failed to lyse an HLA-A2-positive and tyrosinase-expressing melanoma cell line. Thus, our data demonstrate that IL-10-treated DC induce an antigen-specific anergy in cytotoxic CD8(+) T cells, a process that might be a mechanism of tumors to inhibit immune surveillance by converting DC into tolerogenic antigen-presenting cells.  (+info)

Effects of the Chinese traditional medicine mao-bushi-saishin-to on therapeutic efficacy of a new benzoxazinorifamycin, KRM-1648, against Mycobacterium avium infection in mice. (8/7363)

The Chinese traditional medicine mao-bushi-saishin-to (MBST), which has anti-inflammatory effects and has been used to treat the common cold and nasal allergy in Japan, was examined for its effects on the therapeutic activity of a new benzoxazinorifamycin, KRM-1648 (KRM), against Mycobacterium avium complex (MAC) infection in mice. In addition, we examined the effects of MBST on the anti-MAC activity of murine peritoneal macrophages (M phi s). First, MBST significantly increased the anti-MAC therapeutic activity of KRM when given to mice in combination with KRM, although MBST alone did not exhibit such effects. Second, MBST treatment of M phi s significantly enhanced the KRM-mediated killing of MAC bacteria residing in M phi s, although MBST alone did not potentiate the M phi anti-MAC activity. MBST-treated M phi s showed decreased levels of reactive nitrogen intermediate (RNI) release, suggesting that RNIs are not decisive in the expression of the anti-MAC activity of such M phi populations. MBST partially blocked the interleukin-10 (IL-10) production of MAC-infected M phi s without affecting their transforming growth factor beta (TGF-beta)-producing activity. Reverse transcription-PCR analysis of the lung tissues of MAC-infected mice at weeks 4 and 8 after infection revealed a marked increase in the levels of tumor necrosis factor alpha, gamma interferon (IFN-gamma), IL-10, and TGF-beta mRNAs. KRM treatment of infected mice tended to decrease the levels of the test cytokine mRNAs, except that it increased TGF-beta mRNA expression at week 4. MBST treatment did not affect the levels of any cytokine mRNAs at week 8, while it down-regulated cytokine mRNA expression at week 4. At week 8, treatment of mice with a combination of KRM and MBST caused a marked decrease in the levels of the test cytokines mRNAs, especially IL-10 and IFN-gamma mRNAs, although such effects were obscure at week 4. These findings suggest that down-regulation of the expression of IL-10 and TGF-beta is related to the combined therapeutic effects of KRM and MBST against MAC infection.  (+info)

TY - JOUR. T1 - Engineered T regulatory type 1 cells for clinical application. AU - Gregori, S. AU - Roncarolo, MG. PY - 2018. Y1 - 2018. N2 - T regulatory cells, a specialized subset of T cells, are key players in modulating antigen (Ag)-specific immune responses in vivo. Inducible T regulatory type 1 (Tr1) cells are characterized by the co-expression of CD49b and lymphocyte-activation gene 3 (LAG-3) and the ability to secrete IL-10, TGF-β, and granzyme (Gz) B, in the absence of IL-4 and IL-17. The chief mechanisms by which Tr1 cells control immune responses are secretion of IL-10 and TGF-β and killing of myeloid cells via GzB. Tr1 cells, first described in peripheral blood of patients who developed tolerance after HLA-mismatched fetal liver hematopoietic stem cell transplantation, have been proven to modulate inflammatory and effector T cell responses in several immune-mediated diseases. The possibility to generate and expand Tr1 cells in vitro in an Ag-specific manner has led to their ...
Purified anti-human/mouse IL-21 Antibody - Interleukin 21 (IL-21) is a potent immunomodulatory cytokine mainly produced by NKT and CD4+ T-cells, particularly the inflammatory Th17 subset and has pleiotropic effects on both innate and adaptive immune responses.  These actions include positive effects such as enhancing proliferation of NK cells and cytotoxic T cells, and inhibitory effects on the antigen-presenting function of dendritic cells.  It can also be proapoptotic for B cells and NK cells.  Recent studies have shown that IL-21 is also an autocrine cytokine that potently induces T(H)17 differentiation and suppresses Foxp3 expression, and serves as a target for treating inflammatory diseases..
Purified anti-human/mouse IL-21 Antibody - Interleukin 21 (IL-21) is a potent immunomodulatory cytokine mainly produced by NKT and CD4+ T-cells, particularly the inflammatory Th17 subset and has pleiotropic effects on both innate and adaptive immune responses.  These actions include positive effects such as enhancing proliferation of NK cells and cytotoxic T cells, and inhibitory effects on the antigen-presenting function of dendritic cells.  It can also be proapoptotic for B cells and NK cells.  Recent studies have shown that IL-21 is also an autocrine cytokine that potently induces T(H)17 differentiation and suppresses Foxp3 expression, and serves as a target for treating inflammatory diseases..
IL10 Signaling Pathways information: Interleukin-10 (IL-10) is an anti-inflammatory cytokine with important immunoregulatory functions. It is a cytokine with potent anti-inflammatory properties, re
Surprisingly, treatment with PDC-E294-108/IAb- or CYPD353-367/IAb-NPs, which in EAE mice without AIH led to TR1 cell recruitment to the CLNs and EAE reversal (Figure 2, D and E; and Supplemental Figure 2A), resulted in the accumulation of these cells in the liver and liver-draining LNs but not in the CLNs (Supplemental Figure 5, A-C). As a result, PDC-E294-108/IAb-NPs and CYPD353-367/IAb-NPs suppressed liver disease (Figure 3, C and D; and Supplemental Figure 5D), but not EAE (Figure 3, A and B). Additional experiments in a NOD model of AIH (Ad-hFTCD induced) confirmed that recruitment of such ubiquitous antigen-reactive TR1-like cells to the liver was antigen driven; treatment of these mice with BDC2.5mi/IAg7-NPs triggered cognate TR1 cell recruitment to the PLNs and PCLNs, but not to the CLNs, MLNs, or liver (Supplemental Figure 5, E-G). Thus, accumulation of pMHC-NP-induced TR1 cells to sites of inflammation and draining lymphoid tissue and the ensuing therapeutic effects require local ...
Objective: This study evaluated the expression of pro-inflammatory (IL-1 beta, IL-6, IFN-gamma and TNF-alpha) and anti-inflammatory (IL-4 and TGF-beta ...
TY - JOUR. T1 - Plasma levels of the immunomodulatory cytokine interleukin-10 during normal human pregnancy: a longitudinal study. AU - Holmes, VA. AU - Wallace, Julie. AU - Gilmore, WS. AU - McFaul, P. AU - Alexander, HD. PY - 2003/3. Y1 - 2003/3. N2 - Pregnancy is proposed to be a Th2 phenomenon, where Th2 cytokines inhibit Th1 responses to improve foetal Survival. The importance of interleukin-10 (IL-10), an immunomodulatory cytokine produced by Th2 cells, in the maintenance of normal pregnancy is becoming increasingly apparent. In a longitudinal case-control study, the physiological effect of pregnancy on plasma IL-10 was investigated. The plasma concentration of IL-10 was determined using an ELISA technique in 99 pregnant women sampled at 12. 20 and 35 weeks of gestation, 38 non-pregnant control subjects sampled in parallel and in a subgroup of women sampled at 3 days post-partum (it. pregnant 21, non-pregnant 21). Plasma IL-10 was significantly higher in pregnant women at 12, 20 and 35 ...
Mycobacterium tuberculosis (Mtb), the causative agent of tuberculosis (TB), remains the worlds most successful human bacterial pathogen. Lung granulomas are the pathologic hallmark of TB. The cellular components of the granulomas play an important role in containment and progression of Mtb infection. The balance of pro and anti-inflammatory cytokines within granulomas is associated with reduction in bacterial burden. IL-27 is an immunomodulatory cytokine that modulates T cells. The properties of IL-27 as an enhancer or silencer of inflammation are yet to be evaluated in the context tuberculosis in a non-human primate (NHP) model. We used macaques, a NHP model that recapitulates human TB, to characterize the expression and modulatory effects of IL-27, especially at an individual granuloma level. In this preliminary evaluation, we characterized the cell types producing IL-27 in response to Mtb and in granulomas and correlated IL-27 production with pro and anti-inflammatory cytokines such as ...
Neutrophils are among the first responders at sites of infection and are an essential arm of the innate immune system. They attack invading pathogens through a number of mechanisms, including phagocytosis and the release of lytic enzymes and reactive oxygen species, leading to an acute proinflammatory response (see commentary by Cassatella et al.). Zhang et al. investigated the responses of mouse neutrophils to bacteria and various agonists of pattern-recognition receptors, such as Toll-like receptors (TLRs) and C-type lectin receptors (CLRs). Whereas individual TLR agonists (particularly Pam3, which stimulates TLR2) activated neutrophils in vitro, they did not induce the production of proinflammatory cytokines; rather, they stimulated neutrophils to produce small quantities of the anti-inflammatory cytokine interleukin-10 (IL-10). Exposure of neutrophils to bacteria resulted in the production of much greater amounts of IL-10, which led the authors to investigate which other signals might ...
In the original report describing GAG [7], it was shown that GAG has anti-inflammatory effects in mice. However, the mechanism through which GAG elicits its immunomodulatory effects remained a question at that time. In the present study, we demonstrate that GAG induces its anti-inflammatory effects by inducing the potent anti-inflammatory cytokine IL-1 receptor antagonist.. IL-1Ra can inhibit the activation of the IL-1 pathway by binding to the IL-1R type 1 receptor and prevents recruitment of the IL-1R accessory protein that is required for signalling. It has been repeatedly shown that IL-1 is an essential proinflammatory cytokine of the innate immunity. A deficient IL-1 pathway is also detrimental for the host, since it is an important protective pathway required to fight infection [17]. Thus the IL-1 axis is a potent pro-inflammatory pathway that needs to be tightly regulated, and IL-1Ra is a crucial player in this regulation. Therefore, it is rather surprising that the role of IL-1Ra in ...
Results In the cytokine profile of HIV-1 infected people the increased levels of pro-inflammatory cytokine TNF-α compared to controls (group I - (0.77 ± 0.08), group II - (2.34 ± 0.69), healthy controls - (0.51 ± 0.32) pg/mL, p , 0.05) and the anti-inflammatory IL-10 (group I - (3.99 ± 0.99), group II - (20.08 ± 0.44), healthy controls - (1.68 ± 0.32) pg/mL, p , 0.001) were demonstrated. No significant difference in IL-4 between surveyed troops and comparison group was found.. Patients with CD4 T lymphocyte levels ≤ 200 cells/µL showed significantly higher plasma concentration of TNF-α and IL-10 compared with the group I (p , 0.05). Among HIV-1 infected from group II mean serum concentrations of TNF-α higher than that of group I in 3 times (p , 0.05). A significant increase in the concentration of IL-10 detected in patients with severe immunodeficiency (IL-10 levels in group II was 5 times higher, p , 0.05), which may indirectly indicate a more active involvement of IL-10 during ...
Rabbit anti Human Interleukin-10 antibody recognizes human IL-10 (Interleukin-10), an 18.5kDa immunosuppressive cytokine originally known
Pro- and anti-inflammatory cytokines play a key role in protecting from the pathogenesis of Leishmania infection, and their balance and dynamic changes ..
IL-25). A member of the IL-17 family of immunoregulatory cytokines (IL-17E; SF20, a homodimer of 177 aa subunits), produced by Th2 cells. It has a role in allergic inflammation by up-regulating IgG and IgE production, eosinophil levels, and inflammatory responses, through induction of IL-4, IL-5, and IL-13. ...
Determination of IL-1β, IL-6, TNF-α, MIP-1α, IL-10, TGF-β1 and FGFb levels in the cell-free supernatant obtained from monocytes from healthy subjects (CG, n
TY - JOUR. T1 - Persistent anti-inflammatory cytokine release in septic shock. AU - Sfeir, Tacla. AU - Saha, Dhanonjoy. AU - Astiz, Mark. AU - Rackow, Eric. PY - 1999/1/1. Y1 - 1999/1/1. N2 - Introduction: During septic shock dysregulation of monocyte cytokine release contributes to immunosuppression. We evaluated the difference in release of the pro-inflammatory cytokine tumor necrosis factor (TNF-α) and the anti-inflammatory cytokine interleukin 10 (IL-10) from blood monocytes isolated from healthy individuals and septic shock patients. Methods: Blood mononuclear cells from healthy volunteers (n=10, Normal) and septic shock patients (n=10, SS) were isolated and cultured. Culture plate adherent monocytes were incubated with either medium (Control) or LPS (LPS) for 18 hours at 31°C. The supernatant was collected and analyzed for TNF-α and IL-10 by ELISA. Values are mean ± SEM, * p,0.05 vs. control Results TNF- α ng/ml Normal SS Control .54±.12 .82±.25 LPS 3.26±1* 1.26±.31 IL-10 pg/ml ...
For humans it is crucial to control lipid homeostasis to avoid development of metabolic disorders. Dyslipidemia is a considerable risk factor for development of cardiovascular and liver diseases, such as non-alcoholic fatty liver disease (NAFLD). Infection and inflammation induce the acute phase response (APR), leading to multiple alternations in lipid and lipoprotein metabolism, mediated by changed cytokine production, especially tumor necrosis factor (TNF)-a, interleukin (IL)-1 and IL-6. Another cytokine IL-10 has anti-atheromatous and anti-inflammatory effects, and it has been postulated that IL-10 has gene therapeutic potential. However, the effect of IL-10 on liver and its metabolic functions are still unclear. In the present study, we investigated whether IL-10 could modulate lipid homeostasis in TNF-a- and IL-6-stimulated hepatocytes. We demonstrated that IL-6 increased expression of genes involved in hepatic fatty acid (FA) synthesis [SREBP1a, FAS], FA oxidation [PPARa, CPT1a, CPT2], FA ...
In this study, we have demonstrated that the pan-HDI LAQ824, by inhibiting IL-10 and increasing the expression of B7.2 and the production of several proinflammatory mediators, induced inflammatory macrophages that effectively activate Ag-specific CD4+ T cells and restore the responsiveness of anergic T cells.. Among the above changes, the most striking effect of LAQ824 was its ability to inhibit the production of the immunosuppressive cytokine IL-10. Such an effect was also displayed by other members of the hydroxamic acid family like LBH589, TSA, and SAHA, but not by the more specific HDI MS-275, which mainly target class I HDACs. The central role of IL-10 in the establishment and maintenance of T cell anergy (34, 35, 42-44) prompted us to further investigate the underlying mechanism(s) by which these particular HDIs inhibit IL-10 production in macrophages.. The dynamic production of pro- and anti-inflammatory mediators at the site of Ag encounter has been shown to shape the initiation, ...
In many different cell types, pro-inflammatory agonists induce the expression of cyclooxygenase 2 (COX-2), an enzyme that catalyzes rate-limiting steps in the conversion of arachidonic acid to a variety of lipid signaling molecules, including prostaglandin E₂ (PGE₂). PGE₂ has key roles in many early inflammatory events, such as the changes of vascular function that promote or facilitate leukocyte recruitment to sites of inflammation. Depending on context, it also exerts many important anti-inflammatory effects, for example increasing the expression of the anti-inflammatory cytokine interleukin 10 (IL-10), and decreasing that of the pro-inflammatory cytokine tumor necrosis factor (TNF). The tight control of both biosynthesis of, and cellular responses to, PGE₂ are critical for the precise orchestration of the initiation and resolution of inflammatory responses. Here we describe evidence of a negative feedback loop, in which PGE₂ augments the expression of dual specificity phosphatase 1, ...
B cells like the one pictured here produce tiny packages of an anti-inflammatory molecule that could be used to treat autoimmune diseases, according to new IRP research.
IL-10 is an immunomodulatory cytokine with a critical role in limiting inflammation in immune-mediated pathologies. The mechanisms leading to IL-10 expression by CD4(+) T cells are being elucidated, with several cytokines implicated. We explored the effect of IL-4 on the natural phenomenon of IL-10 production by a chronically stimulated antigen-specific population of differentiated Th1 cells. In vitro, IL-4 blockade inhibited while addition of exogenous IL-4 to Th1 cultures enhanced IL-10 production. In the in vivo setting of peptide immunotherapy leading to a chronically stimulated Th1 phenotype, lack of IL-4Rα inhibited the induction of IL-10. Exploring the interplay of Th1 and Th2 cells through co-culture, Th2-derived IL-4 promoted IL-10 expression by Th1 cultures, reducing their pathogenicity in vivo. Co-culture led to upregulated c-Maf expression with no decrease in the proportion of T-bet(+) cells in these cultures. Addition of IL-4 also reduced the encephalitogenic capacity of Th1 ...
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Dr. Center is Boston Universitys Associate Provost for Translational Research and the Director of the Clinical and Translational Research Institute funded by the NIH. As a result he directs Boston Universitys efforts to facilitate translational research in all venues and leads a major effort in identifying new areas of development.. His own laboratory is interested in two major themes which revolve around roles for Interleukin-16, co-discovered with Bill Cruikshank in 1982. The first theme relates to the functions of IL-16 as a immunomodulatory cytokine. Over the past several years, in collaboration with Bill Cruikshank, he has studied the role of IL-16 in recruitment and development of Regulatory T cells and demonstrated that it plays a key role in tolerance to airborne allergens. Utilizing transgenic knockout and overexpressing mice his laboratory is involved in demonstrating the patterns of CD4+ T cell trafficking through lymph nodes and lung parenchymal in normal and immunologically ...
Walter and Eliza Hall Institute scientists have developed a new drug-like molecule that can halt inflammation and has shown promise in preventing the progression of multiple sclerosis (MS).
During the first treatment pro-inflammatory cytokines IL-1b, TNF-a, IL-6 and IL-8 levels decreased, anti-inflammatory cytokines IL-4, IL-13 were constant within the normal range, IL-10 and MCP-1 levels decreased 10-fold to about normal ...
In this report we describe the generation of mice deficient in IL-13Rα2 to define the role of this receptor chain in IL-13 responses. IL-13Rα2 may act to modulate the effects of IL-13 in vivo in various ways. IL-13Rα2 could enhance IL-13 activities by increasing the strength of IL-13 signaling or attenuate IL-13 effects by negative signaling or simply as a molecular decoy. Attenuating roles of IL-13Rα2 could explain the lack of evidence for IL-13 effects on T cells or an enhancing role could explain the effect of IL-13 effect on airways hyperreactivity and eosinophil survival distinct from IL-4.. Interestingly, we find that the absence of IL-13Rα2 correlates with nearly complete loss of serum IL-13 and an increase in tissue IL-13 in IL-13Rα2−/− mice. The lack of serum IL-13 cannot be explained by a lack of IL-13 production in IL-13Rα2−/− mice as IL-13 is present in tissues of IL-13Rα2−/− and is produced by activated IL-13Rα2−/− immune cells. Serum IL-13Rα2 may act as a ...
PAN Czytelnia Czasopism, The inflammatory reaction and the expression of pro- and anti-inflammatory cytokines to a novel polyester-polyurethane aortic prosthesis evaluated in dog sat 6 and 12 months post-implantation - Polish Journal of Veterinary Sciences
Invitrogen™ eBioscience™ Mouse IL-6 ELISA Ready-SET-Go!™ Kit 2 x 96 tests Invitrogen™ eBioscience™ Mouse IL-6 ELISA...
The immature B cells that successfully make it through this process (only about 10%) enter into the bloodstream and migrate to the spleen. The immature B cells have both IgM and IgG (BCRs) expressed on their surface at this point and once they enter into the spleen are called transitional type 1 (T1) B cells. In the spleen B cells, T cells, and follicular dendritic cells, form what is known as a primary follicle or sometimes the white pulp (See Figure 2). The T1 B cells are located outside of the follicle (extrafollicular) in an area known as the red pulp (as this is where all of the red cells are flowing through the spleen). At this point, the T1 B cells are exposed to more self cells and circulating proteins and if they respond strongly it would indicate autoreactivity and the cells are typically induced to become T3 B cells, which are anergic (which means they become non-responsive to antigen), and will likely die off.. If the T1 B cells survive through this they can then migrate into the ...
有多种机制认为病毒感染与过敏性炎症相互作用,从而导致下呼吸道功能障碍、喘息和哮喘。首先,潜在的过敏性炎症可以直接增强气道对鼻病毒感染的反应性。此外,病毒感染可损害气道上皮的屏障功能,导致气道壁对气传过敏原的吸收增加和炎症反应增强,而潜在的过敏性炎症也可能导致病毒复制增强。值得注意的是,鼻病毒感染和变应原均可促进气道上皮细胞产生IL-33, IL-33是最近发现的一种先天细胞因子,可促进2型气道炎症和重塑。据报道,这种类固醇耐药途径在难以控制哮喘的儿童中上调。有趣的是,IL-33多聚物与中晚期发作的喘息有关,而中晚期发作的喘息与早期生活中的过敏反应密切相关。 另一种先天上皮细胞因子IL-25也由鼻病毒诱导,在过敏患者鼻病毒感染的情况下,IL-25可能加重过敏性气道炎症 ...
Инфламмасома - важный компонент нативного иммунитета. Она представляет собой макромолекулярный комплекс, включающий сенсорные элементы, адапторные белки и зимоген каспазы-1. Под действием продуктов распада тканей и патогенных микроорганизмов инфламмасома активируется и превращает про-IL-1b и про-IL-18 в активные интерлейкины. Активация инфламмасом отмечена при многих воспалительных заболеваниях и служит мишенью для терапевтических воздействий. В настоящем обзоре обсуждается вклад инфламмасом в патогенез социально-значимых заболеваний ...
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Fundamental knowledge about the development of the spinal cord is essential to devise approaches for functional spinal cord repair, comprising neuroprotection after injury, replacement of lost neurons and glia, and axonal regeneration.. Pablo Villoslada (IDIBAPS, Barcelona, Spain) presented regenerative therapies for spinal cord injury and multiple sclerosis (MS) in which axonal damage, demyelination and clinical signs are closely correlated, including MESEMS (https://clinicaltrials.gov/ct2/show/NCT01854957), which is currently recruiting participants for a trial of neuroprotection by mesenchymal stem cell (MSC) administration. Similarly, Dearbhaile Dooley (Hendrix lab, Hasselt University, Belgium) has transplanted MSCs, virally transduced to express the TH2 anti-inflammatory cytokine interleukin 13, rostral to a spinal lesion site in the mouse. Dooley observed a reduction in lesion size and demyelination, potentially mediated by a decrease in the number of microglia/macrophages and increased ...
Inflammation is a beneficial mechanism that is usually triggered by injury or infection and is designed to return the body to homeostasis. However, uncontrolled or sustained inflammation can be deleterious and has been shown to be involved in the etiology of several diseases, including inflammatory bowel disorder and asthma. Therefore, effective anti-inflammatory signaling is important in the maintenance of homeostasis in the body. However, the inter-play between pro- and anti-inflammatory signaling is not fully understood. In the present study, we develop a mathematical model to describe integrated pro- and anti-inflammatory signaling in macrophages. The model incorporates the feedback effects of de novo synthesized pro-inflammatory (tumor necrosis factor alpha; TNF-a) and anti-inflammatory (interleukin-10; IL-10) cytokines on the activation of the transcription factor nuclear factor kappaB (NF-kB) under continuous lipopolysaccharide (LPS) stimulation (mimicking bacterial infection). In the ...
Fingerprint Dive into the research topics of Anti-inflammatory IL-10 is upregulated in both hemispheres after experimental ischemic stroke: Hypertension blunts the response. Together they form a unique fingerprint. ...
Acute psychosocial stress stimulates transient increases in circulating pro-inflammatory plasma cytokines, but little is known about stress effects on anti-inflammatory cytokines or underlying mechanisms. We investigated the stress kinetics and interrelations of pro- and anti-inflammatory measures on the transcriptional and protein level.,br /,,br /,Forty-five healthy men were randomly assigned to either a stress or control group. While the stress group underwent an acute psychosocial stress task, the second group participated in a non-stress control condition. We repeatedly measured before and up to 120 min after stress DNA binding activity of the pro-inflammatory transcription factor NF-κB (NF-κB-BA) in peripheral blood mononuclear cells, whole-blood mRNA levels of NF-κB, its inhibitor IκBα, and of the pro-inflammatory cytokines interleukin (IL)-1ß and IL-6, and the anti-inflammatory cytokine IL-10. We also repeatedly measured plasma levels of IL-1ß, IL-6, and IL-10.,br /,,br /,Compared ...
Atherosclerosis is recognized as a complex multifactorial process and a chronic inflammatory disease contributed to severe cardiovascular event, which can be triggered by various stimuli [18]. Among them, TGF-β as a represented immunomodulatory cytokine is of particular interest to cardiovascular biologists through regulation of multiple cell types involved in blood vessel wall [6]. Notably, accumulative evidences have demonstrated that TGF-β exerts an important role in atherogenesis [19,20], whereas the studies of TGF-β receptor modulation account for the effect remains unclear. Members of the TGF-β family of cytokines signal exert the functional regulation of the pathological process via serine/threonine kinase transmembrane type I and type II receptors at the cell membrane [6]. Binding TGF-β to the receptor complex triggers activation of type I receptor terms as ALK, which can initiate downstream signaling including Smad phosphorylation, MAPK and PI3K-Akt signaling, while all three ...
Like interleukin-27 (IL-27), IL-23 is a recently discovered member of the IL-6/IL-12 family of proinflammatory and immunoregulatory cytokines. It exists as a heterodimer composed of the IL-12p40 subunit and a novel p19 subunit. IL-23 is secreted by activated dendritic cells, macrophages, and monocytes. Its biological activities include enhancing the proliferation of memory T cells and the production of IFN-gamma, IL-12, and TNF-alpha from activated T cells, and can stimulate macrophages to produce TNF-alpha and nitric oxide. It has also been shown to possess potent anti-tumor and anti-metastatic activity in mouse models of cancer, suggesting a potential role for IL-23 in therapeutic treatment of cancer. ...
Type 2 diabetes is associated with an increased risk of cardiovascular disease. A series of metabolic risk factors have been proposed to account for the accelerated development of atherosclerosis in patients with this disease. In addition, in recent years, chronic inflammation has received increasing attention as an important pathophysiological mechanism in both type 2 diabetes and atherosclerosis. Therefore, genetic variability in factors involved in the regulation of inflammation may have a particular impact on the progression of atherosclerosis in patients with type 2 diabetes.. The interleukin-1 (IL-1) pathway is a central mediator of inflammatory reactions. Increased activation of the IL-1 proinflammatory axis has been reported in diabetes, and IL-1 is also involved in atherogenesis by a multitude of mechanisms. The IL-1 gene cluster contains the genes encoding the proinflammatory cytokines IL-1α and -1β and the anti-inflammatory cytokine IL-1 receptor antagonist (IL-1ra). Among the ...
The cytokine interleukin-22 (IL-22), which is a member of the IL-10 family, is produced exclusively by immune cells and activates signal transducer and activator of transcription 3 (STAT3) in nonimmune cells, such as hepatocytes, keratinocytes, and colonic epithelial cells, to drive various processes central to tissue homeostasis and immunosurveillance. Dysregulation of IL-22 signaling causes inflammatory diseases. IL-22 binding protein (IL-22BP; encoded by IL22RA2) is a soluble IL-22 receptor, which antagonizes IL-22 activity and has genetic associations with autoimmune diseases. Humans have three IL-22BP isoforms, IL-22BPi1 to IL-22BPi3, which are generated by alternative splicing; mice only have an IL-22BPi2 homolog. We showed that, although IL-22BPi3 had less inhibitory activity than IL-22BPi2, IL-22BPi3 was more abundant in various human tissues under homeostatic conditions. IL-22BPi2 was more effective than IL-22BPi3 at blocking the contribution of IL-22 to cooperative gene induction with ...
NEX232 Interleukin-1 (IL-1) refers to a group of three polypeptides (interleukin-1 alpha (IL-1α), interleukin-1 beta (IL-1β) and interleukin-1 receptor antagonist (IL-1Ra)), that play a central role in the regulation of immune and inflammatory responses.. IL-1β is a pro-inflammatory cytokine involved in immune defense against infection. ...
We hear constant chatter from the regulatory types about the dangers of raw milk and the health hazards of consuming it. But they seem to be strangely uninterested in examining the health risks of consuming conventionally produced and processed milk products, not to mention many other processed foods. The L.A. Times article excerpted below gives…
Interleukin-6 (IL-6), 1 mg. Interleukin-6 is a potent pro-inflammatory cytokine primarily produced by activated T cells and an assortment of other cells including endothelial cells and macrophages.
Interleukin-6 (IL-6), 2 µg. Interleukin-6 is a potent pro-inflammatory cytokine primarily produced by activated T cells and an assortment of other cells including endothelial cells and macrophages.
Study Finds Cytokine IL-23 to Play Crucial Role in Autoimmune Diseases. An international group of scientists have now found that the cytokine IL-23 is heavily involved in autoimmune and inflammatory diseases, thereby opening the door to new therapies targeting the molecule.
Interferon-gamma is a regulatory cytokine secreted by activated Th1 Cells which promotes phagocytosis of internalized bacteria by Macrophages. This cytokine is also thought to play an important role in the development of Giant Cells and Granulomas ...
CCL4, CCL20, IFN-γ, IL-2, IL-4, IL-10, IL-16, IL-17A, IL-17F, IL-18, MIF, TNSF15, IL-1β, IL-1R, IL-6, IL-7, IL-7R, IL-8, IL-10R, IL-12p35, IL-12p40, IL-3, IL-15, IL-16, IL-21, IL-21R, IL-22, IL-17D, LITAF, NK-lysin, CD25, CD80, CD83, CD86, IFN-α, IFN-r, TGFB4, B-defensin8 ...
人IL-17 ELISA试剂盒(Interleukin-17) ELISA试剂盒datasheet (ab100556).Abcam抗体、ELISA、激动剂拮抗剂、表观遗传试剂、蛋白多肽,使用效果保证,中国70%以上现货。
人IL-2 ELISA试剂盒(Interleukin-2) ELISA试剂盒datasheet (ab100566).Abcam抗体、ELISA、激动剂拮抗剂、表观遗传试剂、蛋白多肽,使用效果保证,中国70%以上现货。
IL-12/IL-23 p40, Functional Grade, clone: C8.6, eBioscience™ 500μg; Functional Grade IL-12/IL-23 p40, Functional Grade, clone: C8.6, eBioscience™...
Serological cytokine expression for IL-1β over the time course.IL-1β levels were detected at all the allocated time points with a significant difference betwe
Receptors, Interleukin-4, Type I information including symptoms, causes, diseases, symptoms, treatments, and other medical and health issues.
产品名称:HumanIL-5ELISAReady-SET-Go规格:10plates货号:88-7056-88厂商:eBioscience产品介绍:Alsoknownas:Interleukin-5ReactivityHumanSensitivity4pg/mLStandardCurveRange4-500pg/mLComponentsCaptureAntibody.Pre-titrated,pu
She also discovered that dendritic cells produce the interleukin essential for activation of T-cells (IL-12) and subsequent ... She was the first to discover the immunosuppressive functions of Interleukin-10 (IL-10), which inhibits antigen presentation by ... Moore, Kevin W.; de Waal Malefyt, Rene; Coffman, Robert L.; O'Garra, Anne (2001). "Interleukin-10And Theinterleukin-10 Receptor ... is a British immunologist who has made important discoveries on the mechanism of action of Interleukin 10. O'Garra was born in ...
Marchant, A.; Devière, J; Byl, B; De Groot, D; Vincent, JL; Goldman, M. (1994). "Interleukin-10 production during septicaemia ... Goriely, S; Neurath, MF; Goldman, M (2008). "How microorganisms tip the balance between interleukin-12 family members". Nature ...
Epstein-Barr virus encodes an interleukin protein with high sequence identity to the human interleukin-10, suggesting a recent ... Fickenscher, H; Hör, S; Küpers, H; Knappe, A; Wittmann, S; Sticht, H (February 2002). "The interleukin-10 family of cytokines ...
There is some evidence that interleukin-10 may inhibit it in an animal model. It is classified as type II lupus nephritis by ... "Interleukin-10 inhibits experimental mesangial proliferative glomerulonephritis". Clinical & Experimental Immunology. 128 (1): ...
Akdis, Cezmi (1998). "Role of interleukin 10 in specific immunotherapy". Journal of Clinical Investigation. 102 (1): 98-106. ...
"A physical map of the region encompassing the human interleukin-1 alpha, interleukin-1 beta, and interleukin-1 receptor ... The protein encoded by this gene is a member of the interleukin 1 cytokine family. This gene and eight other interleukin 1 ... Interleukin-1 family member 10 is a protein that in humans is encoded by the IL1F10 gene. ... Bensen JT, Dawson PA, Mychaleckyj JC, Bowden DW (Dec 2001). "Identification of a novel human cytokine gene in the interleukin ...
Asseman C, Mauze S, Leach MW, Coffman RL, Powrie F (October 1999). "An essential role for interleukin 10 in the function of ... The main mechanism is a production of anti-inflammatory cytokine interleukin 10 (IL-10). The regulatory effects of Bregs were ... Mauri C, Gray D, Mushtaq N, Londei M (February 2003). "Prevention of arthritis by interleukin 10-producing B cells". The ... induction by interleukin 4 (IL-4) and IL-10". Infection and Immunity. 70 (2): 812-9. doi:10.1128/iai.70.2.812-819.2002. PMC ...
Among ten known human RAET1/ULBP genes, six encode functional proteins: RAET1E/ULBP4, RAET1G/ULBP5, RAET1H/ULBP2, RAET1/ULBP1, ... "Interleukin 10 decreases MICA expression on melanoma cell surface". Immunology and Cell Biology. 89 (3): 447-57. doi:10.1038/ ... 3 (10): 781-90. doi:10.1038/nri1199. PMID 14523385. S2CID 18234848. Li P, Morris DL, Willcox BE, Steinle A, Spies T, Strong RK ...
Cancer of T cells is termed T-cell lymphoma, and accounts for perhaps one in ten cases of non-Hodgkin lymphoma. The main forms ... Singh B, Schwartz JA, Sandrock C, Bellemore SM, Nikoopour E (2013). "Modulation of autoimmune diseases by interleukin (IL)-17 ... Brooks DG, Trifilo MJ, Edelmann KH, Teyton L, McGavern DB, Oldstone MB (November 2006). "Interleukin-10 determines viral ... Treg cells can be a source of IL-10 and TGF-β and therefore they can play a role in T cell exhaustion. Furthermore, T cell ...
Tosato, G; Jones, Kd (March 1990). "Interleukin-1 induces interleukin-6 production in peripheral blood monocytes". Blood. 75 (6 ... Taga, K; Mostowski, H; Tosato, G (June 1993). "Human interleukin-10 can directly inhibit T-cell growth". Blood. 81 (11): 2964- ... Sgadari, C; Angiolillo, Al; Tosato, G (May 1996). "Inhibition of angiogenesis by interleukin-12 is mediated by the interferon- ... inducible protein 10". Blood. 87 (9): 3877-3882. doi:10.1182/blood.V87.9.3877.bloodjournal8793877. ISSN 0006-4971. PMID 8611715 ...
Secretion of Interleukin-10 Genetically engineered L. lactis can secrete the cytokine interleukin-10 (IL-10) for the treatment ... Stordeur P, Goldman M (1998). "Interleukin-10 as a regulatory cytokine induced by cellular stress: molecular aspects". Int. Rev ... 1998). "Suppression of T cell-mediated injury in human gut by interleukin 10: role of matrix metalloproteinases". ... "A phase I trial with transgenic bacteria expressing interleukin-10 in Crohn's disease". Clin Gastroenterol Hepatol. 4 (6): 754- ...
A related suggestion with regard to interleukin 2 is that activated regulatory T cells take up interleukin 2 so avidly that ... These include Transforming growth factor beta, Interleukin 35, and Interleukin 10. It also appears that regulatory T cells can ... Annacker O, Asseman C, Read S, Powrie F (June 2003). "Interleukin-10 in the regulation of T cell-induced colitis". Journal of ... Toll interleukin-1 receptor domain, which is normally present in innate immune cells. The immune system must be able to ...
"Posttranscriptional regulation of interleukin-10 expression by hsa-miR-106a". Proc. Natl. Acad. Sci. U.S.A. 106 (14): 5761-6. ... 89 (10): 1037-50. doi:10.1007/s00109-011-0775-x. PMID 21656380. S2CID 26039378. Wang Z, Liu M, Zhu H, Zhang W, He S, Hu C, Quan ...
Liu J, Zhang H, Liu Y, Wang K, Feng Y, Liu M, Xiao X (October 2007). "KLF4 regulates the expression of interleukin-10 in ... 10: 99. doi:10.1186/1476-4598-10-99. PMC 3173388. PMID 21851624. Wu A, Luo W, Zhang Q, Yang Z, Zhang G, Li S, Yao K (April 2013 ... 10 (8): 1312-21. doi:10.4161/cc.10.8.15363. PMID 21490434. Sureban SM, May R, Qu D, Weygant N, Chandrakesan P, Ali N, Lightfoot ... 10 (1): 100-25. doi:10.1111/j.1582-4934.2006.tb00293.x. PMC 3933104. PMID 16563224. Ohnishi S, Ohnami S, Laub F, Aoki K, Suzuki ...
In 2013, after a student occupation in favour of ten demands, including fair pay for workers, a halt to privatisation of the ... discoveries on the mechanism of action of Interleukin 10 (Anne O'Garra); the formulation of the theory of electromagnetism ( ... "News_5-10-2006-13-17-17". imperial.ac.uk. Attwood, Rebecca. "London trio to award their own degrees". Times Higher Education ... 10 January 2004. Retrieved 13 May 2017. "London UOTC". Ministry of Defence. Archived from the original on 8 May 2017. Retrieved ...
Interleukin 10 knock out mice develop invasive adenocarcinoma in the colon. Mutant mice for interleukin 2 and beta ... 1996). "Enterocolitis and colon cancer in interleukin-10-deficient mice are associated with aberrant cytokine production and ... After 8-10 months, 45% to 56% of the mice developed colonic adenocarcinomas, and no mice had cancers of the small intestine. On ... 10 (12): 1433-42. doi:10.1101/gad.10.12.1433. PMID 8666228. Edelmann W, Yang K, Umar A, et al. (1997). "Mutation in the ...
Interleukin-10 is an anti-inflammatory cytokine that contributes to the homeostatic maintenance of the organism by controlling ... Iyer, Shankar Subramanian; Cheng, Genhong (2012). "Role of Interleukin 10 Transcriptional Regulation in Inflammation and ... it has been demonstrated that thanks to esculentin-2CHa mouse lymphoid cells stimulate the production of interleukin-10. ... Retrieved 2017-10-22. Simmaco M, Mignogna G, Barra D, Bossa F (April 1994). "Antimicrobial peptides from skin secretions of ...
... such as interleukin 4 (IL-4) and interleukin 13 (IL-13). TAMs gather in necrotic regions of tumors where they are associated ... Among such strategies are antibodies to the interleukin-10 receptor (IL10R). In a mammary carcinoma mouse model it neutralized ... 10 (1): 120-7. doi:10.1016/S0959-437X(99)00043-X. PMID 10679388. Sinkus R, Lorenzen J, Schrader D, Lorenzen M, Dargatz M, Holz ... The stroma comprises a variable portion of the entire tumor; up to 90% of a tumor may be stroma, with the remaining 10% as ...
... a molecular basis for species divergence in interleukin-10-induced anti-inflammatory actions". Mol. Cell. Biol. 27 (13): 4603- ... "Adiponectin inhibits pro-inflammatory signaling in human macrophages independent of interleukin-10". J. Biol. Chem. 284 (38): ... 33 (10): 2250-3. doi:10.2337/dc10-0452. PMC 2945168. PMID 20628086. Igarashi H, Yahagi A, Saika T, Hashimoto J, Tomita T, ...
"Interleukin-10-secreting type 1 regulatory T cells in rodents and humans". Immunological Reviews. 212: 28-50. doi:10.1111/j. ... High IL-10 production is characteristic for Tr1 cells themselves and they also produce TGF-β. In the presence of IL-10 can be ... Tr1 cells are dependent on IL-10 and TGF-β as well as Tregs, but differ from them by lacking expression of Foxp3. ... 10 (5): 518-524. doi:10.1016/S0952-7915(98)80217-3. ISSN 0952-7915. PMID 9794831. Kendal, Adrian R.; Waldmann, Herman (October ...
Hobbs K, Negri J, Klinnert M, Rosenwasser LJ, Borish L (December 1998). "Interleukin-10 and transforming growth factor-beta ... 13 (10): e0205608. doi:10.1371/journal.pone.0205608. PMC 6192621. PMID 30332484. Lin JM, Collins PJ, Trinklein ND, Fu Y, Xi H, ... 25 (10): 1010-22. doi:10.1101/gad.2037511. PMC 3093116. PMID 21576262. Chen HY, Shao CJ, Chen FR, Kwan AL, Chen ZP (April 2010 ... The sequence at -10 (the -10 element) has the consensus sequence TATAAT. The sequence at -35 (the -35 element) has the ...
In this study, a recombinant strain of L. lactis containing complimentary DNA (cDNA) for the human interleukin-10 (IL-10) gene ... "A Phase I Trial With Transgenic Bacteria Expressing Interleukin-10 in Crohn's Disease". Clinical Gastroenterology and ... Secretion of IL-10 from L. lactis in the gut is considered to be a topical administration of the drug to the epithelium, ... Regarding IL-10 secretions in L. lactis, the delay is acceptable for treatment of IBD, however any drug that requires a precise ...
Interleukin-2 receptor alpha chain (also called CD25) is a protein that in humans is encoded by the IL2RA gene. The interleukin ... "Entrez Gene: IL2RA interleukin 2 receptor, alpha". Triplett, Todd A.; et al. (July 2012). "Defining a functionally distinct ... Leonard WJ, Donlon TA, Lebo RV, Greene WC (Aug 1985). "Localization of the gene encoding the human interleukin-2 receptor on ... Kuziel WA, Greene WC (June 1990). "Interleukin-2 and the IL-2 receptor: new insights into structure and function". J. Invest. ...
"Circulating muramyl dipeptide is negatively associated with interleukin-10 in the frail elderly". Inflammation. 38 (1): 272-277 ...
"A phase I trial with transgenic bacteria expressing interleukin-10 in Crohn's disease". Clinical Gastroenterology and ... doi:10.1160/TH07-10-0593. PMID 18449413. Bryant J, Baxter L, Cave CB, Milne R (July 2007). Bryant J (ed.). "Recombinant growth ... 210: 10-22. doi:10.1016/j.jenvman.2017.12.075. PMID 29329004. Valda D, Dowling J (10 December 2010). "Making Microbes Better ... 10 (1): 72. doi:10.1186/1471-2334-10-72. ISSN 1471-2334. PMC 2845586. PMID 20298555. Karl Drlica (2004). Understanding DNA and ...
W ten sposób zidentyfikowano limfocyty Ts jako populację o niskiej ekspresji białek CD5[16], CD45RB[17] i CD45RC[18]. Problemem ... A function for interleukin 2 in Foxp3-expressing regulatory T cells. „Nat Immunol". 6 (11), s. 1142-1151, listopad 2005. DOI: ... Proces ten ma na celu wyeliminowanie takich limfocytów, które mogą reagować na własne antygeny organizmu[31]. Możliwe są tutaj ... W ten sposób komórki autoreaktywne zostają usunięte[31];. *limfocyt rozpoznaje własny antygen i zostaje skierowany na szlak ...
Cancer of T cells is termed T-cell lymphoma, and accounts for perhaps one in ten cases of non-Hodgkin lymphoma.[54] The main ... "Interleukin-10 determines viral clearance or persistence in vivo". Nature Medicine. 12 (11): 1301-9. doi:10.1038/nm1492. PMC ... Treg cells can be a source of IL-10 and TGF-β and therefore they can play a role in T cell exhaustion.[63] Furthermore T cell ... Through IL-10, adenosine, and other molecules secreted by regulatory T cells, the CD8+ cells can be inactivated to an anergic ...
Myxoma Interleukin 6 Papillary fibroelastoma Rhabdomyomas Cardiac sarcomas Hecht, Sisalee M. (2009-10-27). "A Review of: " ... About 10% of myxomata are inherited, as in Carney syndrome. Such tumors are called familial myxomata. They tend to occur in ... Most myxomata arise sporadically (90%), and only about 10% are thought to arise due to inheritance. ...
Li M, He S (April 2006). "Purification and characterization of recombinant human interleukin-29 expressed in Escherichia coli ... "Interferon-lambda is functionally an interferon but structurally related to the interleukin-10 family". The Journal of ... 41 (10): 1105-9. doi:10.1038/ng.449. PMID 19749757. S2CID 20399078. Muir AJ, Gong L, Johnson SG, Lee MT, Williams MS, Klein TE ... 10 (2): 125-31. doi:10.1038/gene.2008.87. PMID 18987645. Li M, Liu X, Zhou Y, Su SB (July 2009). "Interferon-lambdas: the ...
Production of an interleukin-10, an endogenous, pro-regulatory cytokine, has also been implicated.[citation needed] In ...
Identification of a coordinate regulator of interleukins 4, 13, and 5 by cross-species sequence comparisons.. Science. 2000, ... 10],這顯示轉錄作用背後可能還存在一些未知的機制。此外,不同種類的哺乳動物在演化的過程中共同保留了這些序列,也顯示基因組中還有很多作用未知的部分[11]。人類基因組內大量功能未知的序列,是目前科學
Instead, plasma cells are identified through flow cytometry by their additional expression of CD138, CD78, the Interleukin-6 ... Compared with CD138, which disappears rapidly ex vivo, the expression of CD319 is considerably more stable.[10] ...
Interleukin-10 determines viral clearance or persistence in vivo. Nature Medicine. November 2006, 12 (11): 1301-9. PMC 2535582 ... Modulation of autoimmune diseases by interleukin (IL)-17 producing regulatory T helper (Th17) cells. The Indian Journal of ... 细胞因子IL-10或TGF-β也可以导致耗竭[46][47]。调节T细胞因为可以分泌IL-10和TGF-β,也与T细胞耗竭相关[48]。在阻断PD-1受体并减少调节T细胞数量后,T细胞耗竭的情况可以得到反转[49]。[
EPO " Interleukin " G-CSF " OM " LIF " IFN " CD " TNF-α " LT " Ligand " TGF " MIF ... C5a " CCL2 " CCL5 " GSM-CSF " LPS " MCP-1 " IL-1 " IL-2 " IL-3 " IL-4 " IL-5 " IL-6 " IL-8 " IL-10 " IL-12 " IL-13 " IL-15 " IL ...
... the Antigen-Presenting Cell causes a response in a TH2 lymphocyte which produce the cytokine interleukin-4 (IL-4). The TH2 ... 10] This type of sensitization has been suggested as a possible causal mechanism for chronic pain conditions. The changes of ... They have shown that IgE levels are at their greatest before 10 years of age and fall vastly until one reaches 30.[15] There is ...
CXCL10, hemokin (C-X-C motiv) ligand 10, ili IP-10[1] je mali citokin iz CXC hemokin familije koji je takođe poznat kao 10 kDa ... Farber JM (1997). „Mig and IP-10: CXC chemokines that target lymphocytes.". J. Leukoc. Biol. 61 (3): 246-57. PMID 9060447.. ... Angiolillo (1995). „Human interferon-inducible protein 10 is a potent inhibitor of angiogenesis in vivo". J. Exp. Med. 182: 155 ... CXCL10 - chemokine (C-X-C motif) ligand 10". Архивирано из оригинала на датум 20. 04. 2010. Приступљено 17. 07. 2010.. ...
positive regulation of interleukin-4 production. • oocyte development. • positive regulation of type IV hypersensitivity. • ... 289 (1-2): 10-5. doi:10.1016/j.mce.2008.01.029. PMID 18502569. S2CID 3455166.. ...
Stimulation of interleukin-2 production by substance p". Journal of Neuroimmunology. 37 (1-2): 65-74. doi:10.1016/0165-5728(92) ... 3 (10): 2021-9. PMID 6194276.. *^ Gerard NP, Garraway LA, Eddy RL, Shows TB, Iijima H, Paquet JL, Gerard C (Nov 1991). "Human ... 165 (10): 5606-11. doi:10.4049/jimmunol.165.10.5606. PMID 11067916.. *^ Kearney CJ, Sheridan C, Cullen SP, Tynan GA, Logue SE, ... Subsequently, SP is degraded and NK-1 is re-expressed on the cell surface.[10] ...
The release of proinflammatory cytokines such as interleukin-1 by activated leukocytes triggered by lipopolysaccharides, ... Maier SF, Wiertelak EP, Martin D, Watkins LR (October 1993). "Interleukin-1 mediates the behavioral hyperalgesia produced by ... One major pathway being through stimulation of the nociceptin receptor,[8][9][10] and blocking this receptor may therefore be a ...
Interleukins/T-cell growth factors (see here instead). *Leukemia inhibitory factor (LIF) ... Spreitzer H (13 May 2008). "Neue Wirkstoffe - Tovok". Österreichische Apothekerzeitung (in German) (10/2008): 498.. .mw-parser- ... This page was last edited on 3 January 2020, at 10:44 (UTC). ... 10-12,14,16H,7-9,13H2,1-2H3,(H,30,32)(H,27,28,29)/b4-3+/t16-/m0 ...
proinflamatorni citokin (IL-1, TNF-alfa) • Th1 (INF-gama i TNF-beta) • Th2 (IL-4, IL-5, IL-6, IL-10, IL-13) • Th17 (IL-17,IL-22 ... Za B-ćelijski aktivirajući faktor je bilo pokazano da interaguje sa TNFRSF13B,[7][8] TNFSF13[9] i TNFRSF17.[10][11] ...
proinflamatorni citokin (IL-1, TNF-alfa) • Th1 (INF-gama i TNF-beta) • Th2 (IL-4, IL-5, IL-6, IL-10, IL-13) • Th17 (IL-17,IL-22 ...
... associations and interaction with the interleukin-1alpha-889 C/T polymorphism". Hum. Immunol. 65 (6): 622-31. doi:10.1016/j. ... 43 (10): 2190-5. doi:10.1023/A:1026650118759. PMID 9790453.. *^ Ercilla G, Parés A, Arriaga F, et al. (November 1979). "Primary ... In addition the BAT1 and MICB variant is more common in type 1 diabetes when B8 is absent but DR3 is present[10] These studies ... This compares with 25% of Cw7-B7 that extend to A3::DQ6 [4] Of 25 potential genetic recombinants of A1::DQ2, none exceed 10% of ...
Häusser, J. A., Mojzisch, A., Niesel, M. & Schulz-Hardt, S. (2010). Ten years on: A review of recent research on the job demand ... Theorell, T.; Hasselhorn, H.; Vingård, E.; Andersson, B. (2000). "Interleukin 6 and cortisol in acute musculoskeletal disorders ... 10] *^ Bureau of Labor Statistics. (2004). Civilian labor force (seasonally adjusted)(LNS11000000). Washington, DC: U.S. ... Newsletter of the Society for Occupational Health Psychology, 3, 10. [13] *^ Caruso, C. (2009). NIOSH OHP activities: Training ...
Borges W, Augustine N, Hill H (2000). "Defective interleukin-12/interferon-gamma pathway in patients with ... 10] and environmental allergies are common,[5] and asthma and anaphylaxis has been variably reported.[5] ...
Zeković, Djordje B. (10 October 2008). "Natural and Modified (1→3)-β-D-Glucans in Health Promotion and Disease Alleviation". ... of oat β-glucan at daily amounts of at least 3 grams lowers total and low-density lipoprotein cholesterol levels by 5 to 10% in ...
"Polymorphisms of the interleukin-1 beta gene are associated with increased risk of non-small cell lung cancer". International ... The DPE has been identified in three Drosophila TATA-less promoters and in the TATA-less human IRF-1 promoter.[10] ... The Pribnow box has a 6 bp region centered around the -10 position and an 8-12 bp sequence around the -35 region that are both ... CAAT boxes are rarely found in genes that express proteins ubiquitous in all cell types.[10] ...
... interleukin-5)可促進嗜曙紅顆粒白血球成長及分化。[13] ... 嗜曙紅顆粒白血球細胞核同樣分為多葉(2到4葉)。因在血液中有較強脫粒(英語:degranulation)傾向,嗜曙紅顆粒白血球眾的顆粒數目不定。[12]。,嗜曙紅顆粒白血球的顆粒中包含了特殊的毒性蛋白以及組織蛋白酶(英語:cathepsin
... and interleukin-1 in modulating progesterone and oestradiol production by human luteinized granulosa cells in culture. Hum. ... 多囊卵巢綜合症目前並無特效藥[5]。治療則包括減重和運動等轉變生活型態的方式[11][10],避孕藥物也許對於調整經期、抑制多餘的毛髮生長和青春痘有所幫助。二甲双胍和抗雄性激素可能有所幫
... interleukin 7 receptor chain α deficiency, CD45 deficiency, CD3δ/CD3ε deficiency. T-/B- SCID (both T and B cells absent): RAG 1 ... These new methods have reduced culture time to 10-12 days by using specific cytokines from adult donors or virus-naive cord ... doi:10.1016/S1525-1578(10)60493-X. PMC 1867474 . PMID 15096561. Conley ME, Notarangelo LD, Etzioni A (1999). "Diagnostic ...
Interleukins/T-cell growth factors (see here instead). *Leukemia inhibitory factor (LIF) ... 10 (6): 702-11. doi:10.1038/nn1903. PMID 17515902. S2CID 6486137.. *^ Penzes P, Johnson RC, Sattler R, Zhang X, Huganir RL, ... 107 (4): 306-10. doi:10.1002/ajmg.10133. PMID 11840487.. *^ Zimmer P, Mierau A, Bloch W, Strüder HK, Hülsdünker T, Schenk A, ... 10. Berlin: Springer. pp. 251-7. doi:10.1038/sj.mp.4001613. ISBN 978-0-387-76760-4. . PMID 15583703. S2CID 21206951.. ; see the ...
यह सामग्री क्रियेटिव कॉमन्स ऍट्रीब्यूशन/शेयर-अलाइक लाइसेंस के तहत उपलब्ध है; अन्य शर्ते लागू हो सकती हैं। विस्तार से जानकारी हेतु देखें उपयोग की शर्तें ...
2003). "Role of the pro-inflammatory cytokines tumor necrosis factor-alpha, interleukin-1 beta, interleukin-6 and interleukin-8 ... Činjenica da je interleukin 8 sekrecija povećana sa oksidant stresom, i obrnuto, interleukin 8 uzrokujući regrutovanje ... Interleukin 8 receptor, beta. Reference[uredi - уреди , uredi izvor]. *↑ Wolff B, Burns AR, Middleton J, Rot A (November 1998 ... vezivanje za interleukin-8 receptor. • vezivanje za proteine. • aktivnost hemokina. Ćelijska komponenta. • ekstracelularni ...
positive regulation of interleukin-17 production. • innate immune response. • positive regulation of natural killer cell ...
Rattazzi M, Puato M, Faggin E, et al. (2004). C-reactive protein and interleukin-6 in vascular disease: culprits or passive ... Mastorakos G, Ilias I (2007). Interleukin-6: a cytokine and/or a major modulator of the response to somatic stress. Ann. N. Y. ... Nishimoto N (May 2006). Interleukin-6 in rheumatoid arthritis. Curr Opin Rheumatol 18 (3): 277-81. PMID: 16582692. DOI: 10.1097 ... Smith PC, Hobisch A, Lin DL, Culig Z, Keller ET (March 2001). Interleukin-6 and prostate cancer progression. Cytokine Growth ...
4,0 4,1 4,2 4,3 4,4 4,5 4,6 Kolls JK, Lindén A (2004). "Interleukin-17 family members and inflammation". Immunity 21 (4): 467- ... "Interleukin-23 promotes a distinct CD4 T cell activation state characterized by the production of interleukin-17". J. Biol. ... Interleukin 17 (IL-17, ili IL-17A) je osnivački član grupe citokina poznatih kao IL-17 familija. IL-17A, je originalno (1993. ... 17,0 17,1 17,2 17,3 Moseley TA, Haudenschild DR, Rose L, Reddi AH (2003). "Interleukin-17 family and IL-17 receptors". Cytokine ...
Interleukin receptors *IL2R / IL2RA/IL2RB / IL15R. *IL4R / IL13R / IL13RA1 / IL13RA2. *IL7R / IL7RA ... 10 (4): 267-71. doi:10.1097/00062752-200307000-00003. PMID 12799531.. *. Granados S, Hwang ST (Jun 2004). "Roles for CD30 in ...
Interleukins/T-cell growth factors (see here instead). *Leukemia inhibitory factor (LIF) ...
Singh B, Schwartz JA, Sandrock C, Bellemore SM, Nikoopour E (2013). „Modulation of autoimmune diseases by interleukin (IL)-17 ... Tr1 ćelije su povezane sa IL-10, Th3 ćelije su povezani sa TGF-beta. Nedavno su na ovu listu dodate i Treg17 ćelije.[9] ... Sluzokožno povezani invarijantni T limfociti (MAIT) su posebna vrsta T ćelija, čije postojanje je objavljeno 2009.[10][11][12][ ... Tokom sekrecije IL-10, adenozina i ostalih molekula koje se luče putem regulacijskih T ćelija, CD8+ ćelije mogu preći u ...
Interleukins/T-cell growth factors (see here instead). *Leukemia inhibitory factor (LIF) ... 2011-10-12. Retrieved 2013-12-28.. *^ "New Stanford molecular target for diabetes treatment discovered - Office of ... 10] Upon activation by PDGF, these receptors dimerise, and are "switched on" by auto-phosphorylation of several sites on their ...
... Hensen, P ... BACKGROUND: The anti-inflammatory cytokine interleukin (IL)-10 is considered to play a major role in the pathophysiology of ... The IL-10 promoter region contains a highly polymorphic microsatellite (IL10.G) and in a recent case-control study the IL10.G13 ... Systemic administration of IL-10 has been shown to be an effective therapy for psoriasis. ...
... and ProteinsPeptidesIntercellular Signaling Peptides and ProteinsCytokinesInterleukinsInterleukin-10 ... and ProteinsProteinsIntercellular Signaling Peptides and ProteinsCytokinesInterleukinsInterleukin-10 ... Interleukin-10 combines with itself to form a homodimeric molecule that is the biologically active form of the protein. ... Interleukin-10. A cytokine produced by a variety of cell types, including T-LYMPHOCYTES; MONOCYTES; DENDRITIC CELLS; and ...
2004). "The T-cell lymphokine interleukin-26 targets epithelial cells through the interleukin-20 receptor 1 and interleukin-10 ... Interleukin 10 receptor, beta subunit is a subunit for the interleukin-10 receptor. IL10RB is its human gene. IL10RB has also ... 2000). "Interleukin (IL)-22, a novel human cytokine that signals through the interferon receptor-related proteins CRF2-4 and IL ... Pletnev S, Magracheva E, Wlodawer A, Zdanov A (2006). "A model of the ternary complex of interleukin-10 with its soluble ...
Interleukin-10. In: Thomson AW, Lotze MT eds. Cytokine Handbook. 4th ed, vol 1. London, UK, and San Diego, Calif: Academic ... Interleukin-10 (IL-10) is an important suppressor of immune responses.1-3 In vitro studies indicate that IL-10 directly ... 2. Mosser DM, Zhang X. Interleukin-10: New perspectives on an old cytokine. Immunol Rev. 2008 Dec; 226:205-218. 19161426 ... IL-10 acts as an immunosuppressor of antigen presenting cells (APC). IL-10 suppresses epidermal Langerhans cell APC function, ...
Posttranscriptional regulation of interleukin-10 expression by hsa-miR-106a. Amit Sharma, Manish Kumar, Jyotirmoi Aich, Manoj ... Posttranscriptional regulation of interleukin-10 expression by hsa-miR-106a. Amit Sharma, Manish Kumar, Jyotirmoi Aich, Manoj ... Posttranscriptional regulation of interleukin-10 expression by hsa-miR-106a Message Subject (Your Name) has sent you a message ... Posttranscriptional regulation of interleukin-10 expression by hsa-miR-106a. Amit Sharma, Manish Kumar, Jyotirmoi Aich, Manoj ...
Interleukin-10 and the interleukin-10 receptor.. Moore KW1, de Waal Malefyt R, Coffman RL, OGarra A. ... Interleukin-10 (IL-10), first recognized for its ability to inhibit activation and effector function of T cells, monocytes, and ... IL-10 plays a key role in differentiation and function of a newly appreciated type of T cell, the T regulatory cell, which may ... This review highlights findings that have advanced our understanding of IL-10 and its receptor, as well as its in vivo function ...
Za interleukin 10 je pokazano da ostvaruje interakcije sa Interleukin 10 receptorom, alfa podjedinicom.[9][10][11][12][13] ... vezivanje za interleukin-10 receptor. • vezivanje za proteine. Ćelijska komponenta. • ekstracelularni region. • ekstracelularni ... Interleukin 10 (IL-10 ili IL10), isto poznat kao ljudski inhibitorni faktor sinteze (CSIF), je antiinflamatorni citokin. Kod ... Interleukin 10 je klasifikovan kao klasa 2 citokin. U ovu citokinsku klasu spadaju interleukin-10 (IL-10), IL-19, IL-20, IL-22 ...
Interleukin-10, Human, Recombinant, S. frugiperda; Synonym: rhIL-1αrhIL-1βrhIL-10; find Sigma-Aldrich-407700 MSDS, related peer ... Interleukin-10, Human, Recombinant, S. frugiperda Synonym: rhIL-1αrhIL-1βrhIL-10 ... Recombinant, human interleukin-10 expressed in S. frugiperda insect cells. Pleiotropic cytokine that can exert either ... Recombinant, human interleukin-10 expressed in S. frugiperda insect cells. Pleiotropic cytokine that can exert either ...
Interleukin-12 downregulation, Interleukin-1 beta downregulation, Interleukin-2 Downregulation, Interleukin-4 upregulation, ... Interleukin-4 upregulation, Interleukin-6 Downregulation, Interleukin-8 downregulation, NF-kappaB Inhibitor, Tumor Necrosis ... Interleukin-1 beta downregulation, Interleukin-4 upregulation, Interleukin-6 Downregulation, NF-kappaB Inhibitor, Postaglandin ... Interleukin-1 beta downregulation, Interleukin-4 upregulation, Interleukin-6 Downregulation, Nrf2 activation, Tumor Necrosis ...
IL-10 down-regulates the enhanced secretion as well as messenger RNA levels of proinflammatory cytokines by IBD mononuclear ... In vivo topical application of IL-10 induces down-regulation of proinflammatory cytokine secretion both systemically and ... Immunoregulatory role of interleukin 10 in patients with inflammatory bowel disease Gastroenterology. 1995 May;108(5):1434-44. ... Deficiency of interleukin (IL) 10, a contrainflammatory cytokine, leads to the development of colitis in IL-10 knockout mice. ...
Asthma exacerbation triggered by respiratory virus infection is characterized by increased IL-10 gene expression that may ... Interleukin-10 gene expression in acute virus-induced asthma Am J Respir Crit Care Med. 2005 Aug 15;172(4):433-9. doi: 10.1164/ ... Interleukin (IL)-10 mRNA was significantly increased in virus-infected acute asthma and reduced on recovery from acute asthma. ... Conclusions: Asthma exacerbation triggered by respiratory virus infection is characterized by increased IL-10 gene expression ...
Interleukin-10 Protects Nitric Oxide-Dependent Relaxation During Diabetes. Carol A. Gunnett, Donald D. Heistad, Frank M. Faraci ... Interleukin-10 Protects Nitric Oxide-Dependent Relaxation During Diabetes. Carol A. Gunnett, Donald D. Heistad, Frank M. Faraci ... Interleukin-10 Protects Nitric Oxide-Dependent Relaxation During Diabetes Message Subject (Your Name) has forwarded a page to ... Namiki M, Yamashita T, Takeuchi S, Ozaki M, Sakoda T, Miwa Y, Hirase T, Inoue N, Kawashima S: Gene transfer of interleukin-10 ...
... interleukin 10 include Assessment of Antibody-based Drugs Effects on Murine Bone Marrow and Peritoneal Macrophage Activation ... Interleukin-10 combines with itself to form a homodimeric molecule that is the biologically active form of the protein. ... Interleukin-10: A cytokine produced by a variety of cell types, including T-Lymphocytes; Monocytes; Dendritic cells; and ...
negative regulation of interleukin-6 production Source: UniProtKB. *negative regulation of membrane protein ectodomain ... sp,P46651,IL10_CERAT Interleukin-10 OS=Cercocebus atys OX=9531 GN=IL10 PE=2 SV=1 ... Belongs to the IL-10 family.Curated. Keywords - Domaini. Signal. Family and domain databases. Integrated resource of protein ... IPR000098, IL-10. IPR020443, IL-10/19/20/22/24/26_fam. IPR020423, IL-10_CS. ...
negative regulation of interleukin-12 production Source: Ensembl. *negative regulation of interleukin-6 production Source: ... sp,P51496,IL10_MACMU Interleukin-10 OS=Macaca mulatta OX=9544 GN=IL10 PE=2 SV=1 ... Belongs to the IL-10 family.Curated. Keywords - Domaini. Signal. Phylogenomic databases. evolutionary genealogy of genes: Non- ... IPR000098, IL-10. IPR020443, IL-10/19/20/22/24/26_fam. IPR020423, IL-10_CS. ...
The tuberculosis vaccine induced an interleukin-10 producing T-cell population with no T helper 1 or 2 bias in vitro. While ... BCG stimulated dendritic cells induce an interleukin-10 producing T-cell population with no T helper 1 or T helper 2 bias in ... The DC phenotype was assessed by CD83 expression, interleukin-12 (IL-12) and IL-10 production, as well as for the ability to ... The tuberculosis vaccine induced an interleukin-10 producing T-cell population with no T helper 1 or 2 bias in vitro. While ...
Interleukin-10 (IL-10) is an anti-inflammatory cytokine with a short half-life that plays an important role in re-establishing ... Stable dimeric forms of IL-10, like Fcα-IL-10, may not only be a better format for improved production, but also a more ... By introducing a short glycine-serine-linker between the fourth and fifth alpha helix of human IL-10 a stable monomeric form of ... In this study we show that the major production bottleneck of human IL-10 is not protein instability as previously suggested, ...
"Genetic polymorphisms of interleukin 6 and interleukin 10 in Egyptian patients with systemic lupus eythematosus," Lupus, vol. ... Interleukin 6 and 10 Serum Levels and Genetic Polymorphisms in Children with Down Syndrome. Marlon Fraga Mattos,1 Patrícia ... Figure 1: Interleukin concentrations between the groups. (a) IL-10 serum levels in DS (16.10 pg/ml) and control (14.09 pg/ml) ... M. van Oijen, P. P. Arp, F. J. de Jong et al., "Polymorphisms in the interleukin 6 and transforming growth factor β1 gene and ...
Van Montfrans C, Rodriguez Pena MS, Pronk I, Ten Kate FJ, Te Te Velde AA, Van Deventer SJ. Prevention of colitis by interleukin ... Interleukin 10 gene transfer prevents experimental colitis in rats. Gut. 2000;46:344-9.PubMedCrossRefGoogle Scholar ... Innate and adaptive interleukin-22 protects mice from inflammatory bowel disease. Immunity. 2008;29:947-57.PubMedCrossRefGoogle ... The orphan receptor CRF2-4 is an essential subunit of the interleukin 10 receptor. J Exp Med. 1998;187:571-8.PubMedCrossRef ...
Interleukin-10 contains 5 exons and a span of 5.2 kb of genomic DNA that are mainly formed by monocytes and lymphocytes. The ... Interleukin-10 also identified as cytokine synthesis inhibitory factor (CSIF) that are capable of reducing synthesis of pro- ... Interleukin-10 intervene immunostimulatory that helps to eradicate infectious and noninfectious materials with limited ... An anti-inflammatory cytokine found in humans that are programmed via the IL-10 gene which have the abilities to hold back the ...
2004). "The T-cell lymphokine interleukin-26 targets epithelial cells through the interleukin-20 receptor 1 and interleukin-10 ... Interleukin 10 receptor, beta subunit is a subunit for the interleukin-10 receptor. IL10RB is its human gene.[1] ... 2000). "Interleukin (IL)-22, a novel human cytokine that signals through the interferon receptor-related proteins CRF2-4 and IL ... It is an accessory chain essential for the active interleukin 10 receptor complex. Coexpression of this and IL10RA proteins has ...
Interleukin-2,IL-2]], [[Interleukin 3,IL-3]], [[Tumor necrosis factor-alpha,TNFα]] and [[GM-CSF]] made by cells such as [[ ... Interleukin 10 knockout frail mice develop cardiac and vascular dysfunction with increased age.,ref name="pmid23159957">{{cite ... Infobox_gene}} Interleukin 10 (IL-10), also known as human cytokine synthesis inhibitory factor (CSIF ... The Interleukin 10 Inflammatory Bowel Disease Cooperative Study Group , journal = Gastroenterology , volume = 119 , issue = 6 ...
In inflammation, pro-inflammatory cytokines (that is, tumour necrosis factor-α (TNF-α), interleukin (IL)-1, IL-8, and IL-12) ... Interleukin 10, previously named cytokine synthesis inhibitory factor (CSIF), has, like many other cytokines, multiple ... T cells of the human intestinal lamina propria are high producers of interleukin-10 ... and those secreting immunoregulatory interleukins (IL-4, IL-5, IL-10, and IL-13 by type 2 T helper cells). ...
To evaluate this, the pre- and post-PH expression of TNF, TNF-inducible cytokines (interleukin [IL]-1, IL-6) and cytokines ( ... Kupffer cell depletion abolishes induction of interleukin-10 and permits sustained overexpression of tumor necrosis factor ... 10. Shi Yin, Hua Wang, Ogyi Park, Wei Wei, Jilong Shen, Bin Gao, Enhanced Liver Regeneration in IL-10-Deficient Mice after ... Because IL-10 is known to shorten the half-life of TNF mRNA, these results suggest that Kupffer cell production of IL-10 is an ...
If you are a society or association member and require assistance with obtaining online access instructions please contact our Journal Customer Services team ...
This volume provides a set of reviews dedicated to the biology of Interleukin (IL)-10. It includes chapters on its impor... ... Read Interleukin-10 in Health and Disease by with Rakuten Kobo. ... Interleukin-10 in Health and Disease by Current Topics in ... This volume provides a set of reviews dedicated to the biology of Interleukin (IL)-10. It includes chapters on its importance ... You save $14.91 (10%) and Earn Kobo Super Points!. Youll see how many points youll earn before checking out. Well award them ...
Adiponectin significantly increased interleukin-10 (IL-10) mRNA expression at the transcriptional level within 6 hours and ... Serum level of the antiinflammatory cytokine interleukin-10 is an important prognostic determinant in patients with acute ... Adiponectin Specifically Increased Tissue Inhibitor of Metalloproteinase-1 Through Interleukin-10 Expression in Human ... Adiponectin Specifically Increased Tissue Inhibitor of Metalloproteinase-1 Through Interleukin-10 Expression in Human ...
Mechanism of endotoxin desensitization: involvement of interleukin 10 and transforming growth factor beta.. F Randow, U Syrbe, ... Mechanism of endotoxin desensitization: involvement of interleukin 10 and transforming growth factor beta. ... alpha and interleukin (IL) 10 but not IL-1RA production. These results suggest that LPS tolerance reflects a functional switch ... IL-10 and transforming growth factor (TGF) beta 1 showed additive effects in replacing LPS for induction of LPS ...
The IL-10 has at least one cyteine residue introduced (by substitution) in a position selected form 89 positions. The polymer ( ... The conjugate should exhibit a reduced immunostimulatory activity compared to hIL-10. Use of said conjugate for treating ... Conjugate comprising an interleukin-10 (IL-10) polypeptide and a polymer. ... Pegylated interleukin-10 US9833514B2 (en) 2006-09-28. 2017-12-05. Merck Sharp & Dohme Corp.. Use of pegylated IL-10 to treat ...
L were used to measure their tumor necrosis factor alpha (TNF-α), interleukin (IL)-1β and IL-10 levels using mouse cytokine ... Involvement of Interleukin-10 in the Anti-Inflammatory Effect of Sanyinjiao (SP6) Acupuncture in a Mouse Model of Peritonitis. ... Ten minutes after needle retention or 30 min after DEXA treatment, mice received an intraperitoneal injection of carrageenan ( ... D. M. Mosser and X. Zhang, "Interleukin-10: new perspectives on an old cytokine," Immunological Reviews, vol. 226, no. 1, pp. ...
  • I thought that this could be an interesting component of the book that needed to be expanded into sub-chapters since among all cytokines IL-10 appears to be one in which genetic polymorphism is strongly associated with divergent phenotypes. (ebooks.com)
  • After topical IL-10 enema treatment of three steroid therapy-refractory patients with ulcerative colitis, in vitro release of proinflammatory cytokines from IBD peripheral monocytes as well as LPMNC is dramatically down-regulated. (nih.gov)
  • IL-10 down-regulates the enhanced secretion as well as messenger RNA levels of proinflammatory cytokines by IBD mononuclear phagocytes in vitro. (nih.gov)
  • Interleukin (IL)-10 attenuates expression and/or production of proinflammatory cytokines ( 3 ). (diabetesjournals.org)
  • Circulating levels of proinflammatory cytokines are elevated in hyperglycemia or diabetes ( 10 , 11 ). (diabetesjournals.org)
  • This cytokine acts such as one of most important regulatory interleukins acting in antigen-presenting cells through the inhibition of cytokines and HLA class II [ 9 ]. (hindawi.com)
  • Regulatory cytokines include interleukins (IL), interferons (IFN), tumor necrosis factors (TNF), and growth factors [ 4 ]. (hindawi.com)
  • In inflammation, pro-inflammatory cytokines (that is, tumour necrosis factor-α (TNF-α), interleukin (IL)-1, IL-8, and IL-12) which initiate and perpetuate the activation of immune and non-immune cells are counterbalanced by contra-inflammatory mediators. (bmj.com)
  • According to this scheme, human T helper cells have also been functionally divided into those producing cytokines which augment immune activation (IL-2 and interferon-γ, which are attributed to type 1 T helper cells) and those secreting immunoregulatory interleukins (IL-4, IL-5, IL-10, and IL-13 by type 2 T helper cells). (bmj.com)
  • Finally, the functions of other cytokines of the IL-10 family are presented. (springer.com)
  • To evaluate this, the pre- and post-PH expression of TNF, TNF-inducible cytokines (interleukin [IL]-1, IL-6) and cytokines (transforming growth factor [TGF]β 1 and IL-10) that down-regulate TNF were compared in controls and GdCl-treated rats. (wiley.com)
  • Increased levels of inflammatory cytokines such as interleukin-6 (IL-6) are cross-sectionally and longitudinally associated with poor physical function in older adults. (natap.org)
  • T-lymphocyte subsets and inflammatory cytokines of interleukin-5 and interleukin-10 expression in human nasal polyp tissue [in Chinese]. (thefreedictionary.com)
  • Interleukins (ILs) are a large group of cytokines that are produced mainly by leukocytes, although some are produced by certain phagocytes and auxiliary cells. (acris-antibodies.com)
  • During the long pathogenesis from chronic gastritis to gastric cancer spawned by H. pylori infection, host-activated neutrophils and mononuclear cells can produce not only proinflammatory cytokines such as interleukin (IL)-1β, IL-6, IL-8 and tumor necrosis factor (TNF)-α but also anti-inflammatory cytokines like IL-10. (pubmedcentralcanada.ca)
  • Interleukin-10 inhibits the production of pro-inflammatory cytokines by inhibition of T-helper 1 (Th1) lymphocytes and stimulation of B lymphocytes and Th2 lymphocytes and thus downregulates the inflammatory response [ 10 - 12 ]. (pubmedcentralcanada.ca)
  • Helgi Silm, The interleukin-10 family cytokines gene polymorphisms in plaque psoriasis (Interleukiin-10 perekonna tsütokiniinide geenide polümorfismid naastulise psoriaasi puhul), Tartu Ülikool, Arstiteaduskond. (etis.ee)
  • however, they also produce anti-inflammatory cytokines, such as interleukin-10 (IL-10), to resolve the inflammation. (sciencemag.org)
  • The purpose of this study was to investigate roles of the anti-inflammatory cytokine interleukin (IL) 10 and the proinflammatory cytokines IL-1β and tumor necrosis factor α (TNF-α) in spinal manipulation-induced analgesic effects of neuropathic and postoperative pain. (chiro.org)
  • IL-10 inhibits the expression of proinflammatory cytokines such as IL-1 and TNF-α. (biovendor.com)
  • Expression of a number of cytokines and other immune effector molecules are up-regulated in IL-10-expressing tumors that are growth inhibited. (aacrjournals.org)
  • Loss of IL-10 secretion drives production of inflammatory cytokines by the microenvironment, followed by innate immunity-mediated clearance of Taxdriven leukemic cells. (onmedica.com)
  • Coculture of viable B. burgdorferi in RPMI.B with Mφs resulted in more rapid and significant increases in IL-10 transcripts and secreted proteins than coculture with nonviable B. burgdorferi in RPMI, which corresponded with decreased production of proinflammatory cytokines. (asm.org)
  • Interleukin-10 (IL-10), a cytokine produced by T-helper-2 (Th2) lymphocytes, macrophages, and other cell types, has numerous anti-inflammatory functions including inhibition of the release of cytokines, chemokines, lipid mediators, and oxidants from neutrophils and macrophages. (ahajournals.org)
  • 7 Although much attention has been paid to the ability of IL-10 to regulate various inflammatory cytokines, potential direct effects of IL-10 in the microvasculature (hemodynamic effects, leukocyte adhesion, microvascular dysfunction) have not been examined to date. (ahajournals.org)
  • NHL cells synthesize and secrete antiapoptotic cytokines implicated in drug resistance, including interleukin (IL)-6, IL-10, and tumor necrosis factor α. (aacrjournals.org)
  • Among other cytokines, interleukin-10 (IL-10) is an important multifunctional cytokine . (omicsonline.org)
  • In addition to local damage to the bowel, II/R leads to remote organ dysfunction ( 5 , 6 ), particularly in the lung, resulting in acute lung injury (ALI) ( 3 , 7 ), characterized by an excess elevation of pro-inflammatory cytokines and activated neutrophils ( 8 - 10 ). (spandidos-publications.com)
  • Animal models and clinical data support the concept that interleukin (IL)-1, IL-6, tumor necrosis factor (TNF), and IL-8 are typical cytokines involved in acute inflammation ( 7 ). (spandidos-publications.com)
  • Evaluation of production of cytokines especially IL-10 and IL-12 are important factor in investigation of immune response against a variety of intracellular pathogen, vaccination and other immune reactions. (sid.ir)
  • In the present study, the cytokines induced during this infection determined the level of IL-10 and IL-12 in serum of cattle owners with brucellosis and without brucellosis compared with health controls. (sid.ir)
  • Then 5ee blood sample have been taken from donors and cytokines IL-10 and IL-12 of ELISA were determined by Disclose Company. (sid.ir)
  • However, MMPs are also involved in controlling the availability of active forms of cytokines and growth factors-pro-tumor necrosis factor-α, interleukin (IL)-1β, insulin-like growth factor binding proteins, and FasL can be cleaved by MMPs ( 6 -9 ). (aacrjournals.org)
  • Further investigation has shown that IL-10 predominantly inhibits lipopolysaccharide (LPS) and bacterial product mediated induction of the pro-inflammatory cytokines TNFα, IL-1β, IL-12, and IFNγ secretion from Toll-Like Receptor (TLR) triggered myeloid lineage cells. (wikipedia.org)
  • IL-10 is capable of inhibiting synthesis of pro-inflammatory cytokines such as IFN-γ, IL-2, IL-3, TNFα and GM-CSF made by cells such as macrophages and Th1 T cells. (wikipedia.org)
  • IL-10 is linked to the myokines, as exercise provokes an increase in circulating levels of IL-1ra, IL-10, and sTNF-R, suggesting that physical exercise fosters an environment of anti-inflammatory cytokines. (wikipedia.org)
  • Increased secretion of pro-inflammatory cytokines by circulating polymorphonuclear neutrophils and regulation by interleukin 10 during intestinal inflammation. (uni-kiel.de)
  • PMN are an important source of pro-inflammatory cytokines in patients with intestinal inflammation and can be downregulated by IL-10.PMN from patients with IBD are primed to secrete enhanced amounts of pro-inflammatory cytokines accompanied by detection of corresponding mRNAs in comparison with normal controls. (uni-kiel.de)
  • Interleukin 10 (IL-10) is an important contra-inflammatory cytokine which induces downregulation of pro-inflammatory cytokines. (uni-kiel.de)
  • The large amount of IL-10 secreted by B cells derived from AIDS-related lymphomas suggests that HIV-1 also exerts direct effect on IL-10 secretion. (nih.gov)
  • IL-10 regulation of IL-1 beta, TNF-alpha, and IL-1 receptor antagonist expression by peripheral monocytes or isolated lamina propria mononuclear cells (LPMNC), respectively, was studied by enzyme-linked immunosorbent assay (cytokine secretion) and by semiquantitative reverse-transcription polymerase chain reaction. (nih.gov)
  • IL-10 down-regulates IL-1 beta and TNF-alpha secretion as well as messenger RNA levels in IBD peripheral monocytes and LPMNC in a dose-dependent manner. (nih.gov)
  • In parallel, IL-1 receptor antagonist secretion is induced, and IL-10 can restore diminished in vitro IL-1 receptor antagonist/IL-1 beta ratios in IBD to normal levels. (nih.gov)
  • In vivo topical application of IL-10 induces down-regulation of proinflammatory cytokine secretion both systemically and locally. (nih.gov)
  • The role of IL-10 secretion in patients with UC in determining the clinicopathological outcome of infection merits further study. (hindawi.com)
  • Human IL-10 down regulates transcription and secretion of IL-1β, IL-6, IL-8, TNF-α, and G-CSF by activated monocytes and macro- phages. (bmj.com)
  • Adiponectin significantly increased interleukin-10 (IL-10) mRNA expression at the transcriptional level within 6 hours and significantly increased IL-10 protein secretion within 24 hours. (ahajournals.org)
  • In vitro induced LPS tolerance looks like the ex vivo LPS hyporesponsiveness of monocytes from septic patients with fatal outcome: downregulation of LPS-induced TNF-alpha and IL-10 production but not of IL-1RA secretion. (rupress.org)
  • The secretion of interferon-y (IFN-[gamma]) and interleukin-2 (IL-2) are associated with a resolving infection. (thefreelibrary.com)
  • Interleukin (IL)-10 secretion by tumor cells was demonstrated to be one of the mechanisms by which tumor cells can escape immunological recognition and destruction. (aacrjournals.org)
  • IL-10 decreased the production of IL-6 and the expression of IL-12 in the presence of TNF-α or sCD40L, but it had no effect on IL-15, IL-18, and TNF-α secretion. (aacrjournals.org)
  • On the other hand, the absence of NK cells led to an increased secretion of interleukin-10 upon in vitro stimulation with T. cruzi. (asm.org)
  • In this study, examination of cytokine secretion by NHL 2F7 tumor cells revealed down-regulation of IL-10 by Rituximab treatment. (aacrjournals.org)
  • Several studies have demonstrated that BM-mononuclear cells (MNCs), as well as mesenchymal stem cells (MSCs), have the ability to immunoregulate and improve tissue repair through IL-10 secretion ( 9 - 11 ). (spandidos-publications.com)
  • We show here that the inhibition of the specific IFN-γ response can be at least partially attributed to IL-10 secretion by monocytes. (asm.org)
  • This IL-10 secretion was not associated with polymorphisms at positions −1082, −819, and −592 of the IL-10 gene promoter, suggesting that other genetic or environmental factors affect IL-10 expression and secretion. (asm.org)
  • This specific IFN-γ secretion is at least partially induced by interleukin-12 (IL-12), as suggested by the strong correlation between B. pertussis -specific IFN-γ and IL-12 secretion levels, both in infected and in vaccinated infants ( 15 , 16 ). (asm.org)
  • Therefore, we investigated here whether IL-10 secretion in infants vaccinated with an acellular vaccine against B. pertussis plays a role in the high interindividual variations of the specific IFN-γ responses to B. pertussis antigens. (asm.org)
  • In view of the role of interleukin-10 (IL-10) as an anti-inflammatory cytokine, we tested the notion that SCD osteomyelitis is associated with a reduction in IL-10 secretion and, hence, precipitation of a proinflammatory state. (asm.org)
  • Discovered in 1991 IL-10 was initially reported to suppress cytokine secretion, antigen presentation and CD4+ T cell activation. (wikipedia.org)
  • More specifically, PEGylated recombinant human IL-10 (PEG-rHuIL-10) has been shown to enhance CD8+ T cell secretion of the cytotoxic molecules Granzyme B and Perforin and potentiate T cell receptor dependent IFNγ secretion. (wikipedia.org)
  • IL-10 secretion dropped approximately 10-fold after the first 24 h of culture, and was paralleled by a decrease in messenger RNA. (rupress.org)
  • RESULTS: Depletion of IL-10 from human normal colonic mucosa resulted in an IFN-γ response, characterized by early-stage secretion of mature IL-18 and production of the active form of caspase-1 by macrophages and some epithelial cells. (inserm.fr)
  • Orientia tsutsugamushi inhibits tumor necrosis factor alpha production by inducing interleukin 10 secretion in murine macrophages. (semanticscholar.org)
  • The IL-10 promoter region contains a highly polymorphic microsatellite (IL10.G) and in a recent case-control study the IL10.G13 (144 bp) allele was found to be associated with familial early onset psoriasis (type 1 psoriasis) having a susceptible effect. (diva-portal.org)
  • Also, genetic variations in the 3′ UTR of IL10 have been shown to be associated with IL-10 levels that could lead to disease pathogenesis ( 8 , 9 ). (pnas.org)
  • Interleukin 10 ( IL-10 ili IL10 ), isto poznat kao ljudski inhibitorni faktor sinteze ( CSIF ), je anti inflamatorni citokin . (wikipedia.org)
  • Kod ljudi IL-10 kodiran IL10 genom . (wikipedia.org)
  • Lyophilized Interleukin-10 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IL10 should be stored at 4°C between 2-7 days and for future use below -18°C. (prospecbio.com)
  • In humans, interleukin 10 is encoded by the ''IL10'' gene. (wikidoc.org)
  • It was hypothesised that single nucleotide polymorphisms (SNPs) of the genes encoding IL-10 ( IL10 ) and the α subunit of its receptor ( IL10RA ) are associated with changes in, or value of, forced expiratory volume in one second (FEV 1 ) in smoking-induced chronic obstructive pulmonary disease. (ersjournals.com)
  • The interleukin (IL)-10 gene ( IL10 ) is located at 1q31-32, a region not linked with lung function in COPD linkage studies 4 - 6 . (ersjournals.com)
  • Several single nucleotide polymorphisms (SNPs) and haplotypes of IL10 have been associated with the level of expression of IL-10 8 , 9 . (ersjournals.com)
  • The SNPs of IL10 were associated with FEV 1 in asthmatic subjects 10 . (ersjournals.com)
  • Immunohistochemical analysis of fibrotic livers of mice receiving treatment with IL-10 or its PEGylated forms, revealed a decrease in markers reflecting HSC and KC activation induced by 5PEG-IL10. (rug.nl)
  • QDs, characterized by emission at 605, 655 and 705nm, have been conjugated with anti Interleukin 10 antibodies (IL10). (techconnectworld.com)
  • KLF4 is recruited to the il10 promoter, and small-interfering RNA-mediated knockdown of KLF4 expression blocked IL-10 expression during pneumococcal infection. (ersjournals.com)
  • The bovine candidate genes, interleukin-10 (IL10), IL10 receptor alpha/beta (IL10RA/B), transforming growth factor beta 1 (TGFB1) , TGFB receptor class I/II (TGFBR1/2) , and natural resistance-associated macrophage protein 1 (SLC11A1) were sequenced for SNP discovery using pooled DNA samples, and the identified SNPs were genotyped in a case-control association study comprised of 242 MAP negative and 204 MAP positive Holstein dairy cattle. (pubmedcentralcanada.ca)
  • In humans, IL-10 is encoded by the IL10 gene, which is located on chromosome 1 and comprises 5 exons, and is primarily produced by monocytes and, to a lesser extent, lymphocytes, namely type-II T helper cells (TH2), mast cells, CD4+CD25+Foxp3+ regulatory T cells, and in a certain subset of activated T cells and B cells. (wikipedia.org)
  • A number of studies suggest that IL-10 plays a role in controlling inflammation, autoimmunity, and angiogenesis. (labcorp.com)
  • Cell type-specific regulation of IL-10 expression In inflammation and disease. (labcorp.com)
  • New research published online in The FASEB Journal suggests that the anti-inflammatory molecule IL-10 may do more than just reduce inflammation. (eurekalert.org)
  • To make their discovery, Kim and colleagues used transgenic mice that overexpress an anti-inflammatory cytokine, IL-10, in skeletal muscle, and found that these mice were protected from aging-associated inflammation and insulin resistance in that tissue. (eurekalert.org)
  • IL-10 prevents aging-associated inflammation and insulin resistance in skeletal muscle. (eurekalert.org)
  • The importance of IL-10 in the orchestration of the immune response, and the strong evidence for its posttranscriptional regulation, makes it an attractive candidate for miRNA mediated regulation of inflammation. (pnas.org)
  • Regulatory cytokine interleukin 10 (IL-10) has been proposed as a key molecule involved in the attenuation of chronic inflammation and the ensuing tolerance for these helminth parasites. (mendeley.com)
  • Interleukin (IL)-10, an anti-inflammatory cytokine, preserves endothelial function during acute inflammation. (diabetesjournals.org)
  • IL-10 preserves vascular function, including endothelium-dependent relaxation, during acute inflammation produced by endotoxin ( 4 , 5 ). (diabetesjournals.org)
  • These results suggest that BCG vaccination might result in the development of IL-10-producing DCs as well as IL-10-producing T cells that could contribute to restricting overt inflammation in infants exposed to pathogens and thus lead to lower infant mortality. (greenmedinfo.com)
  • Interleukin-10 intervene immunostimulatory that helps to eradicate infectious and noninfectious materials with limited inflammation. (biology-online.org)
  • This study identified, for the first time, the adiponectin/IL-10 interaction against vascular inflammation. (ahajournals.org)
  • Infection and inflammation induce the acute phase response (APR), leading to multiple alternations in lipid and lipoprotein metabolism, mediated by changed cytokine production, especially tumor necrosis factor (TNF)-a, interleukin (IL)-1 and IL-6. (uio.no)
  • IL-10 levels are reduced in induced sputum from patients with COPD, indicating that this might be a mechanism for increasing lung inflammation 7 . (ersjournals.com)
  • Some aged IL-10-/- mice also developed inflammation in the small intestine. (jci.org)
  • A major role for a pathogenic Th1 response was further suggested by showing that anti-IFNgamma antibody (Ab) treatment significantly attenuated intestinal inflammation in young IL-10-/- mice. (jci.org)
  • When weanlings were treated with IL-10, they failed to develop any signs of intestinal inflammation. (jci.org)
  • The most important results of the research of Adriana Mattos Pinto were: 1) PEGylation of Interleukin-10 improved its pharmacokinetics and decreased inflammation and fibrosis in a model of mice with hepatic injury. (rug.nl)
  • Mice deficient in p35 showed enhanced IL-10 production and were protected from tissue damage in a model of chemical-induced gut inflammation. (sciencemag.org)
  • Hematoxylin and eosin staining, whole-cell patch clamp electrophysiological recordings, immunohistochemistry, and enzyme-linked immunosorbent assay were used to examine the neural inflammation, neural excitability, and expression of c-Fos and PKC as well as levels of IL-1β, TNF-α, and IL-10 in blood plasma, DRG, or the spinal cord. (chiro.org)
  • In this study, we used NSPCs overexpressing IL-10, an immunomodulatory cytokine, in an animal model for CNS inflammation and multiple sclerosis (MS). Intravenous injection of IL-10 transduced neural stem/progenitor cells (NSPC IL-10 ) suppressed myelin oligodendrocyte glycoprotein aa 35-55 (MOG35-55)- induced experimental autoimmune encephalomyelitis (EAE) and, following intravenous injection, NSPC IL-10 migrated to peripheral lymphoid organs and into the CNS. (biomedcentral.com)
  • IL-10 is a cytokine with multiple, pleiotropic, effects in immunoregulation and inflammation. (wikipedia.org)
  • The role of interleukin-10 is different than expected and M2c macrophages seem to play a significant role during the development of the disease. (rug.nl)
  • 1-3 In vitro studies indicate that IL-10 directly inhibits IL-2 and IL-5 production by TH1 and TH2 cells. (labcorp.com)
  • Interleukin-10 plays an important role in the communication between cells and participates in a variety of processes: it inhibits inflammatory responses and attenuates activities in fibroblast-like cells, such as hepatic stellate cells (HSC) in the liver. (rug.nl)
  • In dendritic cells (DCs), which are currently used for vaccination therapies for malignant diseases, IL-10 inhibits IL-12 production and induces a state of antigen-specific anergy in CD4- and CD8-positive T cells. (aacrjournals.org)
  • Together, our results suggest that IL-10 inhibits LPS-induced DA neurotoxicity through the inhibition of PHOX activity in a JAK1-dependent mechanism. (aspetjournals.org)
  • KRP-203 significantly inhibits ongoing IL-10 -/- colitis in part through decreasing the infiltration of lymphocytes at inflammatory sites and by blocking T-helper 1 cytokine production in the colonic mucosa. (aspetjournals.org)
  • Our previous studies determined that infected interlukin-10 −/− (IL-10 −/− ) mice show significantly lower B. burgdorferi levels than wild-type (B6) mice and that IL-10 inhibits innate immune responses critical for controlling B. burgdorferi infection. (asm.org)
  • We investigated whether cytotrophoblasts secrete interleukin 10 (IL-10), a cytokine that potently inhibits alloresponses in mixed lymphocyte reactions. (rupress.org)
  • Thus, the first goal of this study was to examine the anti-inflammatory effects of IL-10 on endothelium-dependent relaxation during diabetes using wild-type (IL-10 +/+ ) and IL-10-deficient (IL-10 −/− ) mice. (diabetesjournals.org)
  • To determine the role of virus virulence in generation of immune responses, we analyzed the modulatory effects of IL-10 on disease severity, virus clearance, and the CD4 + T cell response to infection with a recombinant strain of SINV of intermediate virulence (TE12). (asm.org)
  • In addition, the shift from Th17 to Th1 responses with decreased virus virulence indicates that the effects of IL-10 deficiency on immunopathologic responses in the CNS during alphavirus infection are influenced by virus strain. (asm.org)
  • Some effects of IL-10 are not anti-inflammatory, for example activation of B cells to promote autoantibody production. (prolekare.cz)
  • The inhibitory effects of IL-10 on MMP-2 expression correlated with the suppression of MMP-2 promoter activity. (aacrjournals.org)
  • Finally, reconstitution of ATF3 successfully restored the inhibitory effects of IL-10 on MMP-2 gene expression. (aacrjournals.org)
  • Deficiency of interleukin (IL) 10, a contrainflammatory cytokine, leads to the development of colitis in IL-10 knockout mice. (nih.gov)
  • In IL-10 +/+ and IL-10 −/− mice, blood glucose levels were ∼120 mg/dl after citrate administration and ∼400 mg/dl after streptozotocin administration. (diabetesjournals.org)
  • Responses to acetylcholine also were improved by allopurinol (an inhibitor of xanthine oxidase) in vessels from diabetic IL-10 − /− mice. (diabetesjournals.org)
  • Thus, diabetes produces greater impairment of relaxation to acetylcholine in IL-10 −/− mice than in IL-10 +/ + mice. (diabetesjournals.org)
  • Because increased levels of O 2 − impair endothelium-dependent relaxation during diabetes ( 21 , 22 ), a second goal of these studies was to determine whether xanthine oxidase contributes to impaired endothelium-dependent relaxation during diabetes in IL-10 −/− mice. (diabetesjournals.org)
  • 10 generations onto the C57BL/6 strain to yield mice with a C57BL/6 defined background. (diabetesjournals.org)
  • Therefore, we used C57BL/6 mice as wild-type controls (IL-10 +/+ ) in these experiments. (diabetesjournals.org)
  • Whole-body and skeletal muscle insulin action and fatty acyl-CoA levels in the control, IL-6, IL-6/IL-10, lipid, and lipid/IL-10 mice. (diabetesjournals.org)
  • Insulin-stimulated whole-body and skeletal muscle (gastrocnemius) glucose metabolic flux in the control, IL-6, IL-6/IL-10, lipid, and lipid/IL-10 mice. (diabetesjournals.org)
  • Enterocolitis and colon cancer in interleukin-10-deficient mice are associated with aberrant cytokine production and CD4(+) TH1-like responses. (jci.org)
  • Mechanistic studies have associated uncontrolled cytokine production by activated macrophages and CD4+ Th1-like T cells with the enterocolitis exhibited by IL-10-/- mice. (jci.org)
  • Our studies have also shown that inheritable factors strongly influence the disease susceptibility of IL-10-/- mice. (jci.org)
  • In vivo, during CCl4-induced fibrogenesis in mice, both 5PEG-IL-10 and 20PEG-IL-10 showed a longer circulation time compared to IL-10, which was associated with a significant increased liver accumulation. (rug.nl)
  • Furthermore, we showed that dextran sodium sulfate failed to induce colitis in p35-deficient mice, which was associated with the enhanced production of IL-10 by macrophages. (sciencemag.org)
  • Approach and Results- We created chimeric Ldlr −/− mice with a B cell-specific deficiency in IL-10, and confirmed that purified B cells stimulated with LPS failed to produce IL-10 compared with control Ldlr −/− chimeras. (ahajournals.org)
  • Mice lacking B-cell IL-10 demonstrated enhanced splenic B-cell numbers but no major differences in B-cell subsets, T cell or monocyte distribution, and unchanged body weights or serum cholesterol levels compared with control mice. (ahajournals.org)
  • Conclusions- In contrast to its prominent regulatory role in many immune-mediated diseases and its proposed modulatory role in atherosclerosis, B cell-derived IL-10 does not alter atherosclerosis in mice. (ahajournals.org)
  • Previous studies in mice with a virulent strain (neuroadapted SINV [NSV]) of the alphavirus Sindbis virus (SINV) identified a role for Th17 cells and regulation by interleukin-10 (IL-10) in the pathogenesis of fatal encephalomyelitis (K. A. Kulcsar, V. K. Baxter, I. P. Greene, and D. E. Griffin, Proc Natl Acad Sci U S A 111:16053-16058, 2014, https://doi.org/10.1073/pnas.1418966111 ). (asm.org)
  • The absence of IL-10 during TE12 infection led to longer morbidity, more weight loss, higher mortality, and slower viral clearance than in wild-type mice. (asm.org)
  • More severe disease and impaired virus clearance in IL-10 −/− mice were associated with more Th1 cells, fewer Th2 cells, innate lymphoid type 2 cells, regulatory cells, and B cells, and delayed production of antiviral antibody in the central nervous system (CNS) without an effect on Th17 cells. (asm.org)
  • Therefore, IL-10 deficiency led to more severe disease in TE12-infected mice by increasing Th1 cells and by hampering development of the local B cell responses necessary for rapid production of antiviral antibody and virus clearance from the CNS. (asm.org)
  • The absence of IL-10 led to longer illness, more weight loss, more death, and slower viral clearance than in mice that produced IL-10. (asm.org)
  • The Th1 pathogenic cell subtype that developed in IL-10-deficient mice infected with a less virulent virus was distinct from the Th17 subtype that developed in response to a more virulent virus, indicating a role for virus strain in determining the immune response. (asm.org)
  • In mice, SINV causes encephalomyelitis, the severity of which depends on virus and host factors, including host age and genetic background and virus strain ( 7 - 10 ). (asm.org)
  • Neuroadapted SINV (NSV), a strain obtained by passaging the original isolate AR339 in mouse brain, causes fatal encephalomyelitis in adult C57BL/6 (B6) mice ( 8 , 11 ), while virus derived from the tissue culture-passaged HRSP clone Toto1101 causes little disease even in newborn mice ( 10 ). (asm.org)
  • More importantly, IL-10 failed to protect DA neurons in cultures from mice lacking NADPH oxidase (PHOX), a key enzyme for extracellular superoxide production in immune cells, suggesting the critical role of PHOX in IL-10 neuroprotection. (aspetjournals.org)
  • We now demonstrate that treatment of IL-10-tumor-bearing mice with antibodies to either chemokine partially reverses the therapeutic effect of IL-10. (aacrjournals.org)
  • Recombinant protein was administered twice daily in two equal doses of 20 μg i.p. from day +7-13 after transplantation of 5 × 10 5 410.4 cells to mice. (aacrjournals.org)
  • Antibodies or preimmune sera were administered i.p. (250 μl) to tumor-bearing mice on days +1, +4, and +7 after transplantation of 2 × 10 5 of 66-neo or 66-IL10c.5 cells to syngeneic mice. (aacrjournals.org)
  • KRP-203 was administered for 1 or 4 weeks to IL-10 -/- mice with clinical signs of colitis. (aspetjournals.org)
  • Intravital microscopy was used to examine hemodynamic parameters, leukocyte rolling and adhesion, and microvascular permeability in cremasteric postcapillary venules in wild-type mice and in IL-10-deficient (IL-10 −/− ) mice exposed to lipopolysaccharide (LPS). (ahajournals.org)
  • Doses of LPS (3 or 30 μg/kg, IV), which did not reduce blood pressure and minimally altered microvascular hemodynamic factors in wild-type mice, caused significant reductions in these parameters in IL-10 −/− mice, demonstrating at least a 10-fold increased sensitivity in IL-10 −/− mice to LPS-induced hemodynamic alterations. (ahajournals.org)
  • Furthermore, in response to LPS (30 μg/kg, IV), leukocyte rolling, adhesion, and fluorescein isothiocyanate-albumin extravasation were increased in the IL-10 −/− mice. (ahajournals.org)
  • Leukocyte accumulation into other tissues, such as lung, also was enhanced greatly in IL-10 −/− mice. (ahajournals.org)
  • This was specific to endotoxin, because acute chemotactic stimuli including N-formyl-methionyl-leucyl-phenylalanine elicited similar responses in IL-10 −/− and wild-type mice. (ahajournals.org)
  • Recently, it has been shown that exogenous IL-10 can reduce mortality of septic animals and that IL-10-deficient (IL-10 −/− ) mice have a much greater susceptibility to endotoxemia. (ahajournals.org)
  • Regulation of Trypanosoma cruzi infection in mice by gamma interferon and interleukin 10: role of NK cells. (asm.org)
  • In the same work, we also reported that the passive transfer of maternal IgG can modulate offspring IL-10 production, but at the time we did not evaluate the impact of IL-10 upon this mechanism using IL-10 -/- mice. (termedia.pl)
  • NSPC IL-10 suppressed antigen-specific proliferation and proinflammatory cytokine production of lymph node cells obtained from MOG35-55 peptide immunized mice. (biomedcentral.com)
  • We choose to use IL-10 as an immunomodulatory cytokine to be delivered by NSPCs into the CNS of mice immunized to develop EAE. (biomedcentral.com)
  • Expression of IL-10 from transfected tumor cell lines in IL-10 transgenic mice or dosing with IL-10 leads to control of primary tumor growth and decreased metastatic burden. (wikipedia.org)
  • A study in mice has shown that IL-10 is also produced by mast cells, counteracting the inflammatory effect that these cells have at the site of an allergic reaction. (wikipedia.org)
  • Lack of IL-10 has been shown to cause COX activation and resultant Thromboxane receptor activation to cause vascular endothelial and cardiac dysfunctions in mice. (wikipedia.org)
  • Interleukin 10 knockout frail mice develop cardiac and vascular dysfunction with increased age. (wikipedia.org)
  • IL-10 acts as an immunosuppressor of antigen presenting cells (APC). (labcorp.com)
  • This reputation is due to the experimental observation that IL-10 decreases the function of antigen presenting cells and T helper 1 type immune responses. (ebooks.com)
  • An anti-inflammatory cytokine found in humans that are programmed via the IL-10 gene which have the abilities to hold back the antigen production capacity of antigen presenting cells. (biology-online.org)
  • In addition, recent work has suggested that polymorphisms of the promoter region of IL-10 may segregate propulsions in high and low producers. (ebooks.com)
  • T polymorphisms in the IL-10 gene and the IL-6 and IL-10 serum levels in healthy individuals with and without DS. (hindawi.com)
  • IL-10 concentration was higher in DS children but was not influenced by IL-10 gene polymorphisms. (hindawi.com)
  • The IL-10 serum levels are increased in DS individuals, but IL-10 polymorphisms are not the main factors that influence the IL-10 expression in DS. (hindawi.com)
  • There was no association of polymorphisms of the genes encoding interleukin-10 and the α subunit of its receptor with the rate of decline in, or value of, forced expiratory volume in one second in smoking-induced chronic obstructive pulmonary disease. (ersjournals.com)
  • Association of interleukin-10 gene polymorphisms with severe generalized chronic periodontitis. (thefreedictionary.com)
  • Interleukin-1 beta, interleukin-1 receptor antagonist, interleukin-4 and interleukin-10 gene polymorphisms: relationship to occurrence and severity of rheumatoid arthritis. (thefreedictionary.com)
  • Polymorphisms in the gene encoding bovine interleukin-10 receptor alpha are associated with Mycobacterium avium ssp. (pubmedcentralcanada.ca)
  • Allelic polymorphisms located in the promoter region of IL-10 gene may contribute to the regulation of autoantibodies production. (prolekare.cz)
  • BCG stimulated dendritic cells induce an interleukin-10 producing T-cell population with no T helper 1 or T helper 2 bias in vitro. (greenmedinfo.com)
  • IL-10 is an important immunoregulatory and anti-inflammatory cytokine secreted by macrophages, monocytes, dendritic cells, T helper 1 (Th1) and Th2 lymphocytes, B lymphocytes, cytotoxic T cells, and mast cells [ 7 ]. (hindawi.com)
  • Mesenchymal stem cells (MSCs) can suppress dendritic cells (DCs) maturation and function, mediated by soluble factors, such as indoleamine 2,3-dioxygenase (IDO), prostaglandin E(2) (PGE(2)), and nitric oxide (NO). Interleukin-10 (IL-10) is a common immunosuppressive cytokine, and the downstream signaling of the JAK-STAT pathway has been shown to be involved with DCs differentiation and maturation in the context of cancer. (semanticscholar.org)
  • Mesenchymal stem cells inhibit the differentiation of dendritic cells through an interleukin-6-dependent mechanism. (semanticscholar.org)
  • Differential interleukin-10 (IL-10) and IL-23 production by human blood monocytes and dendritic cells in response to commensal enteric bacteria. (semanticscholar.org)
  • IL-10 suppresses epidermal Langerhans cell APC function, monocyte chemokines expression, and the bactericidal responses of macrophages. (labcorp.com)
  • Interleukin-10 also identified as cytokine synthesis inhibitory factor (CSIF) that are capable of reducing synthesis of pro-inflammatory cytokine such as TNF alpha , IFN gamma , IL-2 and IL-3 by cell macrophages and regulatory T-cells yet it stimulates certain T-cells and mast cells as well as enhances the survival of B cell and antibody production. (biology-online.org)
  • Electrophoretic mobility shift assay and chromatin immunoprecipitation revealed a cis-element that was responsible for activation of the IL-10 promoter by Wt1 in murine macrophages. (sigmaaldrich.com)
  • Conclusions- Adiponectin selectively increased TIMP-1 expression in human monocyte-derived macrophages through IL-10 induction. (ahajournals.org)
  • 2) Specific targeting of Interleukin-10 to the hepatic stellate cells (HSCs) in the fibrotic liver led to an exacerbation of fibrosis via an effect on macrophages. (rug.nl)
  • Additional studies showed that other cell types (M2c macrophages) were activated after delivery of interleukin-10 to HSCs and this then stimulated the fibrotic process. (rug.nl)
  • The CDK5-p35 complex prevented macrophages from producing IL-10 and other anti-inflammatory factors. (sciencemag.org)
  • Interleukin-10 (IL-10) is an important anti-inflammatory cytokine that is produced primarily by macrophages. (sciencemag.org)
  • We investigated mechanisms by which the timing of IL-10 production was controlled in macrophages and found that cyclin-dependent kinase 5 (CDK5) activity was markedly increased in lipopolysaccharide (LPS)-stimulated macrophages through the synthesis of the CDK5-binding partner and activator p35. (sciencemag.org)
  • The transiently active CDK5-p35 complexes limited the LPS-stimulated phosphorylation and activation of various mitogen-activated protein kinases (MAPKs), thereby preventing the premature production of SOCS3 (suppressor of cytokine signaling 3), an inhibitor of inflammatory responses in macrophages, and IL-10. (sciencemag.org)
  • Together, our results suggest that CDK5 enhances the inflammatory function of macrophages by inhibiting the MAPK-dependent production of IL-10. (sciencemag.org)
  • IL-10 is an immunosuppressive cytokine produced by a variety of mammalian cell types including macrophages, monocytes, T cells, B cells and keratinocytes. (biovendor.com)
  • Potas, J, Haque, F, Maclean, F et al 2015, 'Interleukin-10 conjugated electrospun polycaprolactone (PCL) nanofibre scaffolds for promoting alternatively activated (M2) macrophages around the peripheral nerve in vivo', Journal of Immunological Methods, vol. 420, pp. 38-49. (edu.au)
  • To determine whether virulent B. burgdorferi preferentially enhances IL-10 production, we developed an in vitro coculture medium (RPMI.B) in which both B. burgdorferi and primary macrophages (Mφs) remain viable. (asm.org)
  • CONCLUSIONS: Innate immune cells (macrophages and epithelial cells) activate a Th1 cell response in explant cultures of human normal colonic mucosa depleted in IL-10 or TGF-β via distinct, nonredundant pathways. (inserm.fr)
  • Cryotherapy resulted in no change in the levels of IL-10 and significantly increased the IFN-[gamma] levels in our patients with CL. (thefreelibrary.com)
  • These cells remained CD14 positive and expressed high levels of IL-10 receptor (IL-10R), suggesting that IL-10 mediates its effects by up-regulating the IL-10R. (aacrjournals.org)
  • Mig and IP-10 are critical to the therapeutic response resulting from high levels of IL-10, and, furthermore, Mig as a single agent also has tumor-inhibitory activity in a model of breast cancer. (aacrjournals.org)
  • Abstract -The objective of this study was to determine whether endogenous IL-10 is capable of regulating hemodynamic parameters, leukocyte recruitment, and microvascular permeability in response to endotoxin. (ahajournals.org)
  • Interleukin-10 contains 5 exons and a span of 5.2 kb of genomic DNA that are mainly formed by monocytes and lymphocytes . (biology-online.org)
  • Preculture of human peripheral blood monocytes for 24 hours with low concentrations of LPS induced hyporesponsiveness to high-dose LPS rechallenge with respect to tumor necrosis factor (TNF) alpha and interleukin (IL) 10 but not IL-1RA production. (rupress.org)
  • IL-10 is an immunoregulatory protein produced by a number of cell lineages, including TH2 cells, B-cells and activated monocytes. (biovendor.com)
  • Interleukin-10 was predominantly produced by monocytes. (haematologica.org)
  • Immunohistochemical analysis of the skin in active chronic graft- versus -host disease showed that infiltrating CD29 + monocytes might produce interleukin-10. (haematologica.org)
  • In our study, the addition of IL-10 to the cultures containing granulocyte/macrophage-colony stimulating factor and IL-4 with or without LPS completely inhibited the generation of DCs from peripheral blood monocytes. (aacrjournals.org)
  • In contrast, the simultaneous incubation of monocytes with IL-10 and TNF-α or soluble CD40 ligand (sCD40L) resulted in the generation of CD83-positive DCs, induction of nuclear localized RelB, and inhibition of IL-10R up-regulation. (aacrjournals.org)
  • IL-10 can be produced by monocytes upon PD-1 triggering in these cells. (wikipedia.org)
  • Extensive IL-10 locus remodeling is observed in monocytes upon stimulation of TLR or Fc receptor pathways. (wikipedia.org)
  • We characterized IL-10 regulation of proinflammatory cytokine (tumor necrosis factor [TNF] alpha and IL-1 beta) expression in IBD in vitro and in vivo. (nih.gov)
  • Mutation of the Wt1 binding motif abrogated stimulation of the IL-10 promoter by tumor necrosis factor-α (TNFα). (sigmaaldrich.com)
  • Blood samples were analyzed for serum 2 5-hydroxyvitamin D, the inflammatory markers serum C-reactive protein (CRP), tumor necrosis factor-alpha and interleukin-6 (IL-6), and the anti-inflammatory cytokine interleukin-10 (IL-10). (thefreedictionary.com)
  • We therefore analyzed the effects of different activation stimuli including lipopolysaccharide (LPS), tumor necrosis factor (TNF)-α, and CD40 ligation on IL-10 mediated inhibition of DC development and stimulatory capacity. (aacrjournals.org)
  • Innate production of Tumor Necrosis Factor-α and Interleukin-10 is associated with radiological progression of knee osteoarthritis. (bmj.com)
  • Relative risks (RR) with 95% confidence intervals (CI95) of OA progression in relation to quartiles of innate ex vivo production of interleukin (IL)-1β, tumor necrosis factor (TNF)-α, IL-1 receptor antagonist (Ra) and IL-10 were calculated. (bmj.com)
  • IL-10 significantly inhibited LPS-induced production of tumor necrosis factor-α, nitric oxide, and extracellular superoxide in microglia cells. (aspetjournals.org)
  • On repeated rHuIL-12 injections, IL-10 concentrations increased further, whereas the transient increments of IFN-gamma, tumor necrosis factor-alpha, IL-6, and IL-8 concentrations, as well as the fluctuations of the leukocyte subset counts, were tapered. (eur.nl)
  • the early-episode profiles are highlighted by an increased frequency of high tumor necrosis factor alpha (TNF-α) and interleukin-4 (IL-4) producers, while the late-episode profiles are exemplified by increased frequencies of IL-10, IL-6, and IL-2 producers ( 11 ). (asm.org)
  • A relatively new area that I tried to emphasize was the role that polymorphism of the IL-10 regulatory regions may play in the context of autoimmune pathology, cancer, transplantation and aging. (ebooks.com)
  • IL-10 is a regulatory cytokine that plays a central role in limiting host immune response to pathogen. (hindawi.com)
  • These results suggest a novel immune regulatory function of Wt1 in controlling IL-10 gene expression. (sigmaaldrich.com)
  • Schif-Zuck S, Wildbaum G, Karin N. Coadministration of plasmid DNA constructs encoding an encephalitogenic determinant and IL-10 elicits regulatory T cell-mediated protective immunity in the central nervous system. (acris-antibodies.com)
  • Objective- To determine the role of regulatory B cell-derived interleukin (IL)-10 in atherosclerosis. (ahajournals.org)
  • The B cells that produce IL-10 (B10) have their regulatory function dependent on IL-4 production [15], an important Th2 pattern cytokine. (termedia.pl)
  • To yield one-half maximal inhibition [ND50] of the biological activity of Rat IL-10 (30.00 ng/ml), a concentration of 0.45-0.80 µg/ml of this antibody is required. (acris-antibodies.com)
  • Our results show that TNF-α or CD40 ligation can antagonize the IL-10-mediated inhibition on DC function, suggesting that depending on activation stimuli, the presence of IL-10 does not necessarily result in T-cell anergy. (aacrjournals.org)
  • We now demonstrate that systemic administration of recombinant human IL-10 to animals bearing established highly malignant mammary tumors also leads to significant growth inhibition. (aacrjournals.org)
  • IL-10 plays a role not only in immunoregulation and inhibition of pro-inflammatory cytokine synthesis, but also in directly regulating the growth and survival of noninflammatory cells. (spandidos-publications.com)
  • IL-10 may autoregulate its expression via a negative feed-back loop involving autocrine stimulation of the IL-10 receptor and inhibition of the p38 signaling pathway. (wikipedia.org)
  • Here, we show that Ido1 activity sustains an immunostimulatory potential through inhibition of interleukin (Il)10. (uu.nl)
  • When the Janus tyrosine kinase (JAK) 1 signaling pathway was blocked, IL-10 failed to attenuate LPS-induced superoxide production, indicating that the JAK1 signaling cascade mediates the inhibitory effect of IL-10. (aspetjournals.org)
  • In contrast, EBV-negative B cell lines do not express IL-10 by Northern blot analysis, ELISA or even PCR. (nih.gov)
  • In each patient, a serum sample was drawn to measure IL-10 by the ELISA technique. (hindawi.com)
  • The serum measurement of IL-6 and IL-10 was performed in a subgroup (54 cases and 54 controls) by enzyme-linked immunosorbent assay (ELISA). (hindawi.com)
  • TIMP-1, MMP-9, and IL-10 concentrations of media were measured with enzyme-linked immunosorbent assay (ELISA) kits (Biotrac). (ahajournals.org)
  • To detect Rat IL-10 by indirect ELISA (using 100 µl/well antibody solution) a concentration of 0.5-2.0 µg/ml of this antibody is required. (acris-antibodies.com)
  • We assessed the regulation of the potent anti-inflammatory cytokine interleukin (IL)-10 in pneumococcal infection via Western blot, ELISA and chromatin immunoprecipitation analysis. (ersjournals.com)
  • GCF (Gingival Crevicular Fluid) samples were collected from each patient using micropipette and IL-10 (interleukin -10) levels were analyzed using ELISA. (omicsonline.org)
  • The levels of interleukin-2 (IL-2) and interferon-γ (IFNγ) for type 1 and interleukin-4, interleukin-5 and interleukin-10 (IL-4, IL-5 and IL-10) for type 2 responses were estimated by ELISA in peripheral blood mononuclear cell (PBMC) culture supernatants. (alliedacademies.org)
  • Interleukin-10 combines with itself to form a homodimeric molecule that is the biologically active form of the protein. (nih.gov)
  • [1] IL-10 protein je homodimer . (wikipedia.org)
  • Wilms' tumor protein Wt1 regulates the Interleukin-10 (IL-10) gene. (sigmaaldrich.com)
  • We identified the Wilms' tumor protein, Wt1, as a novel transcriptional activator of the immunosuppressant cytokine interleukin-10 (IL-10). (sigmaaldrich.com)
  • LPS hyporesponsiveness in septic patients was preceded by expression of IL-10 at both the mRNA and protein level. (rupress.org)
  • Recombinant Rat IL-10 is an 18.7 kDa protein of 160 amino acid residues. (biovendor.com)
  • We had shown previously that expression of the CXC chemokines Mig (monokine induced by IFN-γ) and IP-10 (inducible protein 10) is observed in IL-10 transduced but not neo-vector control tumors. (aacrjournals.org)
  • Rabbit polyclonal antibodies to Mig and IP-10 were produced by Biosynthesis, Inc. (Lewisville, TX) using synthetic peptides selected from the IP-10 and Mig protein sequences (CIHIDDGPVRMRAIGK and CISTSRGTIHYKSLKDLKQFAPS, respectively) coupled to carrier protein keyhole limpet hemocyanin. (aacrjournals.org)
  • The OvHVIL-10 protein was shown to be a non-glycosylated, secreted protein of Mr 21 000 with a signal peptidase cleavage site between amino acids 26 and 27 of the nascent peptide. (uzh.ch)
  • Lentivirus vector‑interleukin‑10 green fluorescent protein (LV‑IL‑10‑GFP) was transfected into endothelial progenitor cells (EPCs) in the present study. (spandidos-publications.com)
  • Lentivirus vector-IL-10-green fluorescent protein (LV-IL-10-GFP) and LV-noncontain-GFP (LV-NC-GFP) were obtained from Xi'An Kewei Biological Technology Company (Xi'an, China). (spandidos-publications.com)
  • Distinguishing the acute presentation of osteomyelitis from VOC relies on clinical assessment (fever and pain on admission, swelling of affected limb, and painful sites) and radiological findings (ultrasound scans, magnetic resonance imaging [MRI]), in combination with elevated C-reactive protein (CRP) and white blood cell (WBC) counts ( 5 , 10 ). (asm.org)
  • Our results demonstrate that IL-10 significantly inhibited MMP-2 transcription and protein expression induced by a phorbol ester, phorbol 12-myristate 13-acetate. (aacrjournals.org)
  • The experiments revealed that a cAMP responsive element binding protein (CREB) consensus domain was identified upstream of the 5′ transcriptional start site, which was highly responsive to IL-10-dependent down-regulation of promoter luciferase activity. (aacrjournals.org)
  • The objective of this study was to determine whether STH-infected children from an endemic community had increased circulating IL-10 levels when compared to non-infected children. (mendeley.com)
  • IL-10 stabilises dimerisation of both IL-10R subunits, and exerts a variety of immunoregulatory effects 13 . (ersjournals.com)
  • Particular emphasis will be placed on the immunoregulatory molecule interleukin-10 (IL-10), investigating association with chicken growth and productivity in the face of parasite challenge, as well as its impact on the outcome of co-infection with Campylobacter jejuni . (rvc.ac.uk)
  • Interleukin-10 (IL-10) is an immunoregulatory cytokine, usually considered to mediate downregulation of the inflammatory response in rheumatoid arthritis (RA). (prolekare.cz)
  • IL-3, IL-4, and GM-CSF (although restricted to the CHI2 lymphomas) may be a resun of transformation, IL-6, TNFα, TNF-β, and CSIFIIL-10 may be important B cell derived immunoregulatory molecules In normal Immune responses Including B cell medlated antigen presentation, autocrine growth of B cells, or B cell medlated irnmunosuppression. (gla.ac.uk)
  • Recombinant, human interleukin-10 expressed in S. frugiperda insect cells. (sigmaaldrich.com)
  • 4 We used recombinant human interleukin-10 (IL-10) (Pepro Tech Ec, Inc), monoclonal anti-human IL-10 antibody (Genzyme Techne), and mouse IgG (Sigma). (ahajournals.org)
  • Recombinant Human IL-10 produced in Escherichia coli was a generous gift of Dr. Satwant Narula, Schering-Plough, Kenilworth, NJ. (aacrjournals.org)
  • PURPOSE: Repeated administrations of recombinant human interleukin-12 (rHuIL-12) to cancer patients are characterized by a reduction of side effects during treatment. (eur.nl)
  • Interleukin-10 (IL-10) is an important suppressor of immune responses. (labcorp.com)
  • IL-10 is a key regulator of the immune system that critically determines health and disease. (pnas.org)
  • IL-10 is a key orchestrator of the immune system that has been shown to be antiinflammatory in many model systems ( 1 ). (pnas.org)
  • Interleukin-10 (IL-10) is regarded as an immune suppressant cytokine. (ebooks.com)
  • Surprisingly, however, IL-10 has potent anti-cancer effects since most experimental models demonstrate immune-mediated anti-tumor effects whether IL-10 is locally (tumor transfectant models) or systemically (transgenic models, administration of soluble cytokine) provided. (ebooks.com)
  • Finally, human observations demonstrate that systemic administration of IL-10 to normal volunteers is associated with very little toxicity and pro-inflammatory properties mediated through activation of effector cells of the innate immune response. (ebooks.com)
  • Interleukin 10 (IL-10) is a cytokine implicated in the regulation of human intestinal immune responses. (hindawi.com)
  • Like other primary immunodeficiencies, IL-10 and IL-10 receptor (IL10R) deficiency present with IBD and demonstrate the sensitivity of the intestine to any changes of the immune system. (springer.com)
  • Like IL-4, IL-10 enhances humoral immune responses and attenuates cell-mediated immune reactions. (biovendor.com)
  • In this study, using mesencephalic neuron-glia cultures, we report that both pretreatment and post-treatment of rat mesencephalic neuron-glia cultures with interleukin (IL)-10, a natural immune modulator, reduced lipopolysaccharide (LPS)-induced DA neurotoxicity. (aspetjournals.org)
  • Interleukin (IL)-10 is a pleiotropic cytokine that plays a critical role in the regulation of inflammatory responses and immune reactions, acting on both hematopoietic and nonhematopoietic cells ( Kremlev and Palmer, 2005 ). (aspetjournals.org)
  • 5 6 This multitude of anti-inflammatory properties suggests an important role for IL-10 in controlling cell-mediated immune and inflammatory responses. (ahajournals.org)
  • Indeed, use of IL-10 in the treatment of steroid-refractory Crohn's disease, which is thought to result from dysregulation of cell-mediated immunity, already has shown promise in initial clinical trials, suggesting that exogenous IL-10 is capable of regulating immune responses in vivo. (ahajournals.org)
  • Acquired immune responses are generally modulated by responses from the innate immune system, and specific IFN-γ responses are often modulated by IL-10. (asm.org)
  • In addition, the immunomodulatory capacity of IFN-β treatment in patients with MS is mediated at least in part by induction of IL-10 production by immune cells. (biomedcentral.com)
  • We studied conversion of human mucosal innate immune cells into inflammatory cells and the initiation of a Th1 cell response following loss of IL-10 or TGF-β signaling. (inserm.fr)
  • Utilizing the Kato-Katz method to detect intestinal helminthiases, 10 children were non-infected and 29 were harbouring STH infections by Ascaris lumbricoides, Trichuris trichiura and/or hookworms. (mendeley.com)
  • Equal concentrations of IL-10 are detectable in both normal and IBD intestinal lamina propria biopsy homogenates. (nih.gov)
  • In 3-mo-old mutants, intestinal lesions were most severe in IL-10-/- 129/SvEv and IL-10-/- BALB/c strains, of intermediate severity in the IL-10-/- 129 x C57BL/6J outbreds, and least severe in the IL-10-/- C57BL/6J strain. (jci.org)
  • IL-10 -819 TT genotype is reversely associated with diffuse-subtype risk but not in intestinal-subtype risk. (pubmedcentralcanada.ca)
  • Interleukin (IL)-10 mRNA was significantly increased in virus-infected acute asthma and reduced on recovery from acute asthma. (nih.gov)
  • Repetitive Activator-assisted spinal manipulative therapy significantly reduced simulated neuropathic and postoperative pain, inhibited or reversed the neurochemical alterations, and increased the anti-inflammatory IL-10 in the spinal cord. (chiro.org)
  • The number of interleukin-10 spot-forming cells in patients with active chronic graft- versus -host disease was significantly higher than the number in those with no or inactive chronic graft- versus -host disease. (haematologica.org)
  • Accordingly, anti-IL-10 monoclonal antibodies significantly increased the efficiency of AS/IFNα therapy. (onmedica.com)
  • IL‑6 RNAi was revealed to significantly reduce the expression of IL‑6, which was associated with upregulated IL‑10 expression in lung tissues. (spandidos-publications.com)
  • The skewed balance between IL-10 and IL-12 is associated with their capability to induce T-cell hyporesponsiveness, because a neutralizing antibody to IL-10, exogenous recombinant IL-12 or lipopolysaccharide (LPS) significantly blocked the hyporesponsiveness. (eurekamag.com)
  • Results obtained showed that IL-10 serum levels in SCD osteomyelitis patients were significantly lower than those of control SCD patients. (asm.org)
  • IL-10 production was significantly up-regulated following stimulation with Bifidobacteria longum, but not after exposure to lipopolysaccharide (LPS) or Streptococcus faecium. (lancs.ac.uk)
  • Potential functional allele T/C single nucleotide polymorphism (SNP) of Interleukin 10 (IL-10) promoter -819 (rs1800871) has been implicated in gastric cancer risk. (pubmedcentralcanada.ca)
  • Interindividual variations in IL-10 production can be a consequence of a genetic polymorphism ( 5 , 25 , 26 ), as well as numerous other factors ( 8 , 9 , 17 , 21 ). (asm.org)
  • To examine the association between both individual IL-10 single nucleotide polymorphism -1082 G/A with the production of rheumatoid factors (RFs) in RA patients. (prolekare.cz)
  • Polymerase chain reaction amplification was used for analysis of the promoter polymorphism of the IL-10 gene. (prolekare.cz)
  • Results obtained in this study provide the evidence of an association of the -1082 G/A polymorphism in IL-10 gene promoter with the production of RFs, which are associated with poorer prognosis of RA. (prolekare.cz)
  • B cell IL-10 is confined to a narrow window in the B cell differentiation pathway, and whereas IL-10 expression is detected in mature and preplasmacytic stages, none of the pro-B, pre-B, or myeloma cell lines produce IL-10. (nih.gov)
  • In this study, using a combination of bioinformatics and molecular approaches, we report that microRNA (hsa-miR-106a) regulates IL-10 expression. (pnas.org)
  • Also, the involvement of transcription factors, Sp1 and Egr1, in the regulation of hsa-miR-106a expression and concomitant decrease in the IL-10 expression, has also been demonstrated. (pnas.org)
  • In summary, our results showed that IL-10 expression may be regulated by miR-106a, which is in turn transcriptionally regulated by Egr1 and Sp1. (pnas.org)
  • Therefore, it is likely that IL-10 expression is tightly regulated. (pnas.org)
  • Regulation of IL-10 expression has been studied at the transcriptional and posttranscriptional levels. (pnas.org)
  • Here, we report the identification of a miRNA, hsa-miR-106a, that regulates IL-10 expression. (pnas.org)
  • We also find that Sp1 and Egr1 have an important role in hsa-miR-106a transcription and, thus, indirectly regulate the expression of IL-10 posttranscriptionally. (pnas.org)
  • Identification of hsa-miR-106a As a Regulator of IL-10 Expression. (pnas.org)
  • Asthma exacerbation triggered by respiratory virus infection is characterized by increased IL-10 gene expression that may explain the suppressed eosinophil influx in acute asthma. (nih.gov)
  • The DC phenotype was assessed by CD83 expression, interleukin-12 (IL-12) and IL-10 production, as well as for the ability to polarize T-cell responses. (greenmedinfo.com)
  • The expression of IL-10 is nominal on unmotivated tissues that require stimulating factor by commensal micro-flora where it strongly regulated by transcriptional and post-transcriptional point. (biology-online.org)
  • IL-10 transcripts were increased more than 15-fold upon forced expression of Wt1. (sigmaaldrich.com)
  • The expression of IL-10 is minimal in unstimulated tissues and seems to require triggering by commensal or pathogenic flora. (wikidoc.org)
  • IL-10 expression is tightly regulated at the transcriptional and post-transcriptional level. (wikidoc.org)
  • Moreover, 24 of these 25 hIL-10-producing B cell lines contained the Epstein-Barr virus (EBV) genome, suggesting a relationship between hIL-10 production by human B cell lines and EBV expression. (rupress.org)
  • Cotreatment of adiponectin with anti-IL-10 monoclonal antibody completely abolished adiponectin-induced TIMP-1 mRNA expression. (ahajournals.org)
  • 5) Rook notes that the observed relationship between abundant Acinetobacter and increased expression of interleukin-10 suggests that Hanski's team has documented a genuine case of immunoregulation by environmental bacteria. (thefreedictionary.com)
  • Streptococcus pneumoniae induced IL-10 expression in mouse lungs and human lung epithelial cells. (ersjournals.com)
  • In conclusion, KLF4 is induced in a bacterial DNA-TLR9-Src-dependent manner and regulates IL-10 expression, linking the detection of bacterial DNA by TLR9 to the control of an inflammatory response. (ersjournals.com)
  • Both Mig and IP-10 mRNAs are up-regulated in IL-10-expressing tumors (10 , 19) , and the present study examines the functional role of this increased expression. (aacrjournals.org)
  • Expression of IL-10 receptor in adult SVZ. (biologists.org)
  • Interleukin 10 (IL-10) is an immuno-suppressive cytokine whose expression is activated by Tax. (onmedica.com)
  • Here we show that, in ATL, AS/IFNα-induced abrogation of leukemia initiating cell activity requires IL-10 expression shutoff. (onmedica.com)
  • Furthermore, expression in 2F7 cells of the protective factor, Bcl-2, was shown to be dependent on IL-10 levels and down-regulated by Rituximab. (aacrjournals.org)
  • IL‑10 expression induced by IL‑6 knockdown in lung tissues was additionally detected. (spandidos-publications.com)
  • We investigated the effects of interleukin (IL)-10 on MMP-2 expression in CPTX-1532 human prostate tumor cells. (aacrjournals.org)
  • Immunoblot assays of activating transcription factor (ATF) 3 immunoprecipitates with tyrosine specific antibodies revealed that IL-10 stimulated tyrosine phosphorylation of ATF3 to activate binding to the CREB domain and suppress MMP-2 expression. (aacrjournals.org)
  • Studies with stable, IL-10 transfected CPTX-1532 subclones further showed that IL-10 failed to suppress MMP-2 expression in ATF3-deficient CPTX-1532 cells, where the ATF3 mRNA was destroyed with a DNAzyme oligonucleotide targeting the 5′ region of the mRNA. (aacrjournals.org)
  • Taken together, these data demonstrate the critical role of tyrosine phosphorylated ATF3 and the CREB consensus domain in IL-10 suppression of MMP-2 gene expression in primary human prostate tumor cells. (aacrjournals.org)
  • Additionally, IL-10 expression is extensively regulated at the post-transcriptional level, which may involve control of mRNA stability via AU-rich elements and by microRNAs such as let-7 or miR-106. (wikipedia.org)
  • There is an up regulation of IL-4, IL-5 and IL-10 expression in patients with lymphatic filariasis and a simultaneous decrease in levels of the interferon-gamma and IL-2. (alliedacademies.org)
  • However, the effect of IL-10 on liver and its metabolic functions are still unclear. (uio.no)
  • These results suggest that endogenous IL-10 may be a homeostatic regulator of hemodynamic parameters, leukocyte-endothelial cell interactions, and microvascular dysfunction in response to endotoxin and provide potential mechanisms to explain the protective effect of IL-10 against LPS-induced mortality. (ahajournals.org)
  • The aim of this study was to explore the relationship between IL-10 systemic synthesis and H. pylori infection in patients with ulcerative colitis. (hindawi.com)
  • The aim of this study was to determine the association of HP infection with the synthesis of IL-10 in Mexican patients with UC. (hindawi.com)
  • IL-10 signals through a receptor complex consisting of two IL-10 receptor-1 and two IL-10 receptor-2 proteins. (wikidoc.org)
  • The researchers, from Brigham and Women's Hospital, and Harvard Stem Cell Institute, and collaborators at MIT and Massachusetts General Hospital, inserted modified strands of messenger RNA into connective tissue stem cells--called mesenchymal stem cells--which stimulated the cells to produce adhesive surface proteins and secrete interleukin-10 , an anti-inflammatory molecule. (thefreedictionary.com)
  • coli interleukin-1o for cell culture or antibody production. (gentaur.com)
  • Il-10 is an important factor in understanding autoimmunity and as a target molecule in the development of anti-rejection therapies. (biovendor.com)
  • Thus, IL-10 is an efficient anti-inflammatory cytokine which potently suppresses EAE and is therefore a suitable candidate molecule to be used in our approach. (biomedcentral.com)
  • Thus, the role that interleukin-10 plays in vivo in physiological or pathological conditions remains controversial. (ebooks.com)
  • In summary, our data suggests that IL-10 and TGF-beta mediate the phenomenon of LPS tolerance in vitro and perhaps in vivo (septic patients), too. (rupress.org)
  • In vivo analyses determined that murine skin samples exhibited substantial upregulation of IL-10 within 24 h of injection of B. burgdorferi . (asm.org)
  • These data suggest that CD40-deficient DC producing IL-10, but not IL-12 can induce T-cell hyporesponsiveness in vitro and in vivo. (eurekamag.com)
  • Insofar as the related anti-inflammatory cytokine IL-10 acts as a suppressor of infection-stimulated bone resorption in vivo ( 22 ) and was associated with SCD complications ( 23 ), the aim of this study was to assess the association between IL-10 serum levels and osteomyelitis in children with SCD. (asm.org)
  • Oliveira M, Lira A, Sgnotto F, Inoue A, Duarte A, Victor J. Preconception immunization can modulate intracellular Th2 cytokine profile in offspring: in vivo influence of interleukin 10 and B/T cell collaboration. (termedia.pl)
  • IL-10 messenger RNA was detected in biopsies of the placenta and the portion of the uterus that contains invasive cytotrophoblasts, suggesting that this cytokine is also produced in vivo. (rupress.org)
  • They speculated that the absence of IL-10 in our ARN case was due to obtaining the vitreous sample late in the disease course. (arvojournals.org)
  • D) Generation of tertiary neurospheres in the absence of IL-10 was similar between previously IL-10-treated and untreated secondary neurospheres ( n = 4). (biologists.org)
  • The results suggest that IL-10 levels are higher in patients with gingivitis than whose condition progressed to periodontitis. (omicsonline.org)
  • In this book, I tried to cover not only the molecular biology of IL-10 but also asked to summarize in general terms its postulated role in controlling adoptive and innate immunity. (ebooks.com)
  • In addition, it presents reviews on the mechanisms linking innate microbial recognition to the production of IL-10 and on how IL-10 recognition by its receptor functions. (springer.com)
  • The innate capacity to produce TNF-α and IL-10 upon LPS stimulation is associated with radiological progression of knee OA, even over a relative short follow-up period of two years. (bmj.com)
  • Loss of interleukin-10 activates innate immunity to eradicate adult T cell leukemia initiating cells. (onmedica.com)
  • Interleukin-10 and interferon-Y levels in patients with cutaneous leishmaniasis treated with cryotherapy. (thefreelibrary.com)
  • Please cite this article as: Handjani F, Yousef SR, Saki N, Hamidizadeh N. Interleukin-10 and Interferon-[gamma] Levels in Patients with Cutaneous Leishmaniasis Treated with Cryotherapy. (thefreelibrary.com)
  • Using an enzyme-linked immunospot assay, we measured the spot-forming cells for interferon-γ, interleukin-4, interleukin-10, and interleukin-17 in unstimulated peripheral blood of patients following allogeneic hematopoietic stem cell transplantation. (haematologica.org)
  • The balance between Th1 and Th2 cytokine profiles is regulated mainly by interferon (IFN)-(Th1-related) and interleukin (IL)-4 (Th2-related) production. (termedia.pl)
  • IL-10 secreted by cytotrophoblasts in vitro is bioactive, as determined by its ability to suppress interferon gamma production in an allogeneic mixed lymphocyte reaction. (rupress.org)
  • Pharmacologic inhibitors and antibodies were used to determine the factors involved in the initiation of an interferon (IFN)-γ response following loss of TGF-β or IL-10 signaling. (inserm.fr)
  • IL-10 amplified the upregulation of PPARa, CPT1a, CPT2 and ABCA1 gene expressions mediated by IL-6 and ADRP gene expressions induced by TNF-a. (uio.no)
  • In conclusion, the present study demonstrated that IL‑6 RNAi may protect the lung from ALI induced by II/R, and that this protective role may be associated with upregulation of IL‑10. (spandidos-publications.com)
  • IL-10 upregulation is also mediated by GPCRs, such as beta-2 adrenergic and type 2 cannabinoid receptors. (wikipedia.org)
  • Whole-blood samples were stimulated with lipopolysaccharide (LPS) (10 ng/ml). (bmj.com)
  • Interleukin-10 (IL-10), a potent anti-inflammatory cytokine, attenuates inflammatory response and suppresses various pro-inflammatory mediators ( 6 - 8 ). (spandidos-publications.com)
  • Using a wide panel of EBV positive and EBV negative cell lines, it has been shown that EBV positive B cell lines derived from patients with AIDS and Burkitt's lymphoma (AABCL) secrete large quantities of B cell IL-10, compared with EBV-positive B cell lines obtained from patients with undifferentiated lymphomas of Burkitt's and non-Burkitt's types. (nih.gov)
  • EBV exerts direct effect on the production of B cell IL-10, and purified tonsillar B cells infected with EBV were triggered to secrete IL-10. (nih.gov)
  • Interleukin 10 (IL-10) is a pleiotropic factor that enhances proliferation of activated human B lymphocytes and induces them to secrete high amounts of immunoglobulins. (rupress.org)
  • Here we show that several human B cell lines were able to constitutively secrete human (h)IL-10. (rupress.org)
  • Accordingly, whereas polyclonal activation via triggering of surface immunoglobulins or CD40 antigen induced highly purified normal human B lymphocytes to produce only low (0.3-0.4 ng/ml) but significant amounts of hIL-10, EBV infection induced them to secrete high amounts of hIL-10 (4-9 ng/ml). (rupress.org)
  • Restoration of vasorelaxation with PEG-SOD or allopurinol suggests that the mechanism(s) by which IL-10 preserves endothelium-dependent vasorelaxation involves O 2 − , perhaps by reducing production of O 2 − by xanthine oxidase. (diabetesjournals.org)
  • Following stimulation with CD40 ligand, DCs matured in the presence of BCG showed enhanced IL-10 and diminished IL-12 production. (greenmedinfo.com)
  • Because IL-10 is known to shorten the half-life of TNF mRNA, these results suggest that Kupffer cell production of IL-10 is an important mechanism that down-regulates TNF production during liver regeneration. (wiley.com)
  • Together, these data suggest that the early activation of CDK5 delays the production of IL-10 and the onset of anti-inflammatory processes. (sciencemag.org)
  • Patients in the highest quartile of TNF-α production had a 6-fold increased risk of JSN progression (age, sex and body mass index adjusted RR 6.1, CI95 1.4-9.8) and patients in the highest quartile of IL-10 production had a 4-fold increased risk of JSN progression (age, sex and body mass index adjusted RR 4.3, CI95 1.7-6.2), both in comparison with those patients in the lowest quartile. (bmj.com)
  • Functional assays showed that OvHVIL-10, in a similar way to ovine IL-10, stimulated mast cell proliferation and inhibited macrophage inflammatory chemokine production. (uzh.ch)
  • The addition of anti-interleukin-10 to these cultures resulted in a marked increase in IFN-gamma production. (asm.org)
  • NSPC IL-10 inhibit T-cell activation, proliferation and cytokine production. (biomedcentral.com)
  • Analysis of flow cytometry data by a sensitive subtraction method allowed measurement of production of interleukin (IL)-12 and IL-10 by small numbers of gut DC by intracellular staining. (lancs.ac.uk)
  • Spontaneous IL-10 production averaged 650, 853, and 992 pg/10(6) cells in the first, second, and third trimesters of pregnancy, respectively. (rupress.org)
  • 7 Administration of glial metabolic inhibitors, cytokine antagonists, antiinflammatory cytokine interleukin (IL)-10, or IL-1 receptor antagonist IL-1ra has been shown to attenuate the development of opioid tolerance and to prevent hyperalgesia and allodynia. (asahq.org)
  • To determine the mechanism of IL-10 action, we examined IL-10-dependent promoter activity with luciferase constructs from a 2-kbp promoter region of the human MMP-2 gene. (aacrjournals.org)
  • IL-10 induction involves ERK1/2, p38 and NF-κB signalling and transcriptional activation via promoter binding of the transcription factors NF-κB and AP-1. (wikipedia.org)
  • The tuberculosis vaccine induced an interleukin-10 producing T-cell population with no T helper 1 or 2 bias in vitro. (greenmedinfo.com)
  • IL-10 and transforming growth factor (TGF) beta 1 showed additive effects in replacing LPS for induction of LPS hyporesponsiveness in vitro. (rupress.org)
  • Cytotrophoblasts from all stages of pregnancy produced IL-10 in vitro, but neither placental fibroblasts nor choriocarcinoma (malignant trophoblast) cell lines did so. (rupress.org)
  • Conclusions: We found no strong evidence for an association between STH infection and serum IL-10 concentration levels. (mendeley.com)
  • Based on the primary breath test result, two groups were formed and serum IL-10 was measured. (hindawi.com)
  • This study suggests an association between serum IL-10 and disease severity in patients with UC and HP infection. (hindawi.com)
  • FINDINGS: The mean serum IL-10 level of cattle owners with positive brucellosis (133.6 ± 20.4) and negative brucellosis (55.5 ± 29.2) compared with the health control (16.1 ± 3.2). (sid.ir)
  • Human IL-10 is also active on murine cell lines. (sigmaaldrich.com)
  • Human IL-10 has cross-species reactivity and is active in mouse cell lines. (sigmaaldrich.com)
  • Human IL-10 is active on murine cells, but murine IL-10 is inactive on human cells. (biovendor.com)
  • More recently, PEGylated recombinant murine IL-10 (PEG-rMuIL-10) has been shown to induce IFNγ and CD8+ T cell dependent anti-tumor immunity. (wikipedia.org)
  • A significant moderate negative correlation between IL-10 levels and children's age was found, but no correlations were observed between IL-10 levels and intensity of infection by any of the parasite species investigated. (mendeley.com)
  • Furthermore, addition of exogenous hIL-10, simultaneously to EBV infection, potentiated cell proliferation, whereas a blocking anti-IL-10 antiserum inhibited it. (rupress.org)
  • IL-10-819 TT genotype is not statistically associated with the overall reduced gastric cancer susceptibility in persons with H. pylori infection compared with controls without H. pylori infection. (pubmedcentralcanada.ca)
  • We analyzed the effects of the anti-inflammatory cytokine IL-10 on disease severity and virus clearance after infection with an alphavirus strain of intermediate virulence. (asm.org)
  • Together, these results suggest that viable B. burgdorferi can suppress early Mφ responses during infection by causing increased release of IL-10. (asm.org)
  • 1 2 3 4 IL-10 also suppresses cytokine release from Th1 lymphocytes and mast cells. (ahajournals.org)