Proteins secreted by vertebrate cells in response to a wide variety of inducers. They confer resistance against many different viruses, inhibit proliferation of normal and malignant cells, impede multiplication of intracellular parasites, enhance macrophage and granulocyte phagocytosis, augment natural killer cell activity, and show several other immunomodulatory functions.
The major interferon produced by mitogenically or antigenically stimulated LYMPHOCYTES. It is structurally different from TYPE I INTERFERON and its major activity is immunoregulation. It has been implicated in the expression of CLASS II HISTOCOMPATIBILITY ANTIGENS in cells that do not normally produce them, leading to AUTOIMMUNE DISEASES.
Interferon secreted by leukocytes, fibroblasts, or lymphoblasts in response to viruses or interferon inducers other than mitogens, antigens, or allo-antigens. They include alpha- and beta-interferons (INTERFERON-ALPHA and INTERFERON-BETA).
Specific molecular sites or structures on or in cells with which interferons react or to which they bind in order to modify the function of the cells. Interferons exert their pleiotropic effects through two different receptors. alpha- and beta-interferon crossreact with common receptors, while gamma-interferon initiates its biological effects through its own specific receptor system.
Agents that promote the production and release of interferons. They include mitogens, lipopolysaccharides, and the synthetic polymers Poly A-U and Poly I-C. Viruses, bacteria, and protozoa have been also known to induce interferons.
One of the type I interferons produced by peripheral blood leukocytes or lymphoblastoid cells. In addition to antiviral activity, it activates NATURAL KILLER CELLS and B-LYMPHOCYTES, and down-regulates VASCULAR ENDOTHELIAL GROWTH FACTOR expression through PI-3 KINASE and MAPK KINASES signaling pathways.
Proteins prepared by recombinant DNA technology.
Non-antibody proteins secreted by inflammatory leukocytes and some non-leukocytic cells, that act as intercellular mediators. They differ from classical hormones in that they are produced by a number of tissue or cell types rather than by specialized glands. They generally act locally in a paracrine or autocrine rather than endocrine manner.
One of the type I interferons produced by fibroblasts in response to stimulation by live or inactivated virus or by double-stranded RNA. It is a cytokine with antiviral, antiproliferative, and immunomodulating activity.
An interferon regulatory factor that binds upstream TRANSCRIPTIONAL REGULATORY ELEMENTS in the GENES for INTERFERON-ALPHA and INTERFERON-BETA. It functions as a transcriptional activator for the INTERFERON TYPE I genes.
An interferon regulatory factor that is expressed constitutively and undergoes POST-TRANSLATIONAL MODIFICATION following viral infection. PHOSPHORYLATION of IRF-3 causes the protein to be translocated from the CYTOPLASM to CELL NUCLEUS where it binds DNA, and activates transcription.
Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others.
A family of transcription factors that share an N-terminal HELIX-TURN-HELIX MOTIF and bind INTERFERON-inducible promoters to control GENE expression. IRF proteins bind specific DNA sequences such as interferon-stimulated response elements, interferon regulatory elements, and the interferon consensus sequence.
A ubiquitously expressed heterodimeric receptor that is specific for both INTERFERON-ALPHA and INTERFERON-BETA. It is composed of two subunits referred to as IFNAR1 and IFNAR2. The IFNAR2 subunit is believed to serve as the ligand-binding chain; however both chains are required for signal transduction. The interferon alpha-beta receptor signals through the action of JANUS KINASES such as the TYK2 KINASE.
A signal transducer and activator of transcription that mediates cellular responses to INTERFERONS. Stat1 interacts with P53 TUMOR SUPPRESSOR PROTEIN and regulates expression of GENES involved in growth control and APOPTOSIS.
Established cell cultures that have the potential to propagate indefinitely.
Serum glycoprotein produced by activated MACROPHAGES and other mammalian MONONUCLEAR LEUKOCYTES. It has necrotizing activity against tumor cell lines and increases ability to reject tumor transplants. Also known as TNF-alpha, it is only 30% homologous to TNF-beta (LYMPHOTOXIN), but they share TNF RECEPTORS.
Lymphocytes responsible for cell-mediated immunity. Two types have been identified - cytotoxic (T-LYMPHOCYTES, CYTOTOXIC) and helper T-lymphocytes (T-LYMPHOCYTES, HELPER-INDUCER). They are formed when lymphocytes circulate through the THYMUS GLAND and differentiate to thymocytes. When exposed to an antigen, they divide rapidly and produce large numbers of new T cells sensitized to that antigen.
RNA sequences that serve as templates for protein synthesis. Bacterial mRNAs are generally primary transcripts in that they do not require post-transcriptional processing. Eukaryotic mRNA is synthesized in the nucleus and must be exported to the cytoplasm for translation. Most eukaryotic mRNAs have a sequence of polyadenylic acid at the 3' end, referred to as the poly(A) tail. The function of this tail is not known for certain, but it may play a role in the export of mature mRNA from the nucleus as well as in helping stabilize some mRNA molecules by retarding their degradation in the cytoplasm.
A soluble factor produced by activated T-LYMPHOCYTES that induces the expression of MHC CLASS II GENES and FC RECEPTORS on B-LYMPHOCYTES and causes their proliferation and differentiation. It also acts on T-lymphocytes, MAST CELLS, and several other hematopoietic lineage cells.
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
Agents used in the prophylaxis or therapy of VIRUS DISEASES. Some of the ways they may act include preventing viral replication by inhibiting viral DNA polymerase; binding to specific cell-surface receptors and inhibiting viral penetration or uncoating; inhibiting viral protein synthesis; or blocking late stages of virus assembly.
An interferon regulatory factor that is induced by INTERFERONS as well as LMP-1 protein from EPSTEIN-BARR VIRUS. IRF-7 undergoes PHOSPHORYLATION prior to nuclear translocation and it activates GENETIC TRANSCRIPTION of multiple interferon GENES.
A heterodimeric cytokine that plays a role in innate and adaptive immune responses. Interleukin-12 is a 70 kDa protein that is composed of covalently linked 40 kDa and 35 kDa subunits. It is produced by DENDRITIC CELLS; MACROPHAGES and a variety of other immune cells and plays a role in the stimulation of INTERFERON-GAMMA production by T-LYMPHOCYTES and NATURAL KILLER CELLS.
The relatively long-lived phagocytic cell of mammalian tissues that are derived from blood MONOCYTES. Main types are PERITONEAL MACROPHAGES; ALVEOLAR MACROPHAGES; HISTIOCYTES; KUPFFER CELLS of the liver; and OSTEOCLASTS. They may further differentiate within chronic inflammatory lesions to EPITHELIOID CELLS or may fuse to form FOREIGN BODY GIANT CELLS or LANGHANS GIANT CELLS. (from The Dictionary of Cell Biology, Lackie and Dow, 3rd ed.)
Any of the processes by which nuclear, cytoplasmic, or intercellular factors influence the differential control (induction or repression) of gene action at the level of transcription or translation.
A soluble substance elaborated by antigen- or mitogen-stimulated T-LYMPHOCYTES which induces DNA synthesis in naive lymphocytes.
Interferon inducer consisting of a synthetic, mismatched double-stranded RNA. The polymer is made of one strand each of polyinosinic acid and polycytidylic acid.
The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.
A critical subpopulation of T-lymphocytes involved in the induction of most immunological functions. The HIV virus has selective tropism for the T4 cell which expresses the CD4 phenotypic marker, a receptor for HIV. In fact, the key element in the profound immunosuppression seen in HIV infection is the depletion of this subset of T-lymphocytes.
An encapsulated lymphatic organ through which venous blood filters.
Penetrating, high-energy electromagnetic radiation emitted from atomic nuclei during NUCLEAR DECAY. The range of wavelengths of emitted radiation is between 0.1 - 100 pm which overlaps the shorter, more energetic hard X-RAYS wavelengths. The distinction between gamma rays and X-rays is based on their radiation source.
Bone marrow-derived lymphocytes that possess cytotoxic properties, classically directed against transformed and virus-infected cells. Unlike T CELLS; and B CELLS; NK CELLS are not antigen specific. The cytotoxicity of natural killer cells is determined by the collective signaling of an array of inhibitory and stimulatory CELL SURFACE RECEPTORS. A subset of T-LYMPHOCYTES referred to as NATURAL KILLER T CELLS shares some of the properties of this cell type.
An interferon regulatory factor that represses transcription of TYPE I INTERFERONS and activates transcription of HISTONE H4.
The most well known avian paramyxovirus in the genus AVULAVIRUS and the cause of a highly infectious pneumoencephalitis in fowl. It is also reported to cause CONJUNCTIVITIS in humans. Transmission is by droplet inhalation or ingestion of contaminated water or food.
Morphologic alteration of small B LYMPHOCYTES or T LYMPHOCYTES in culture into large blast-like cells able to synthesize DNA and RNA and to divide mitotically. It is induced by INTERLEUKINS; MITOGENS such as PHYTOHEMAGGLUTININS, and by specific ANTIGENS. It may also occur in vivo as in GRAFT REJECTION.
Subset of helper-inducer T-lymphocytes which synthesize and secrete interleukin-2, gamma-interferon, and interleukin-12. Due to their ability to kill antigen-presenting cells and their lymphokine-mediated effector activity, Th1 cells are associated with vigorous delayed-type hypersensitivity reactions.
The intracellular transfer of information (biological activation/inhibition) through a signal pathway. In each signal transduction system, an activation/inhibition signal from a biologically active molecule (hormone, neurotransmitter) is mediated via the coupling of a receptor/enzyme to a second messenger system or to an ion channel. Signal transduction plays an important role in activating cellular functions, cell differentiation, and cell proliferation. Examples of signal transduction systems are the GAMMA-AMINOBUTYRIC ACID-postsynaptic receptor-calcium ion channel system, the receptor-mediated T-cell activation pathway, and the receptor-mediated activation of phospholipases. Those coupled to membrane depolarization or intracellular release of calcium include the receptor-mediated activation of cytotoxic functions in granulocytes and the synaptic potentiation of protein kinase activation. Some signal transduction pathways may be part of larger signal transduction pathways; for example, protein kinase activation is part of the platelet activation signal pathway.
Soluble factors which stimulate growth-related activities of leukocytes as well as other cell types. They enhance cell proliferation and differentiation, DNA synthesis, secretion of other biologically active molecules and responses to immune and inflammatory stimuli.
A cytokine produced by a variety of cell types, including T-LYMPHOCYTES; MONOCYTES; DENDRITIC CELLS; and EPITHELIAL CELLS that exerts a variety of effects on immunoregulation and INFLAMMATION. Interleukin-10 combines with itself to form a homodimeric molecule that is the biologically active form of the protein.
Strains of mice in which certain GENES of their GENOMES have been disrupted, or "knocked-out". To produce knockouts, using RECOMBINANT DNA technology, the normal DNA sequence of the gene being studied is altered to prevent synthesis of a normal gene product. Cloned cells in which this DNA alteration is successful are then injected into mouse EMBRYOS to produce chimeric mice. The chimeric mice are then bred to yield a strain in which all the cells of the mouse contain the disrupted gene. Knockout mice are used as EXPERIMENTAL ANIMAL MODELS for diseases (DISEASE MODELS, ANIMAL) and to clarify the functions of the genes.
Mature LYMPHOCYTES and MONOCYTES transported by the blood to the body's extravascular space. They are morphologically distinguishable from mature granulocytic leukocytes by their large, non-lobed nuclei and lack of coarse, heavily stained cytoplasmic granules.
Technique using an instrument system for making, processing, and displaying one or more measurements on individual cells obtained from a cell suspension. Cells are usually stained with one or more fluorescent dyes specific to cell components of interest, e.g., DNA, and fluorescence of each cell is measured as it rapidly transverses the excitation beam (laser or mercury arc lamp). Fluorescence provides a quantitative measure of various biochemical and biophysical properties of the cell, as well as a basis for cell sorting. Other measurable optical parameters include light absorption and light scattering, the latter being applicable to the measurement of cell size, shape, density, granularity, and stain uptake.
A nucleoside antimetabolite antiviral agent that blocks nucleic acid synthesis and is used against both RNA and DNA viruses.
The phenotypic manifestation of a gene or genes by the processes of GENETIC TRANSCRIPTION and GENETIC TRANSLATION.
Lipid-containing polysaccharides which are endotoxins and important group-specific antigens. They are often derived from the cell wall of gram-negative bacteria and induce immunoglobulin secretion. The lipopolysaccharide molecule consists of three parts: LIPID A, core polysaccharide, and O-specific chains (O ANTIGENS). When derived from Escherichia coli, lipopolysaccharides serve as polyclonal B-cell mitogens commonly used in laboratory immunology. (From Dorland, 28th ed)
The assay of INTERFERON-GAMMA released from lymphocytes after their exposure to a specific test antigen, to check for IMMUNOLOGIC MEMORY resulting from a previous exposure to the antigen. The amount of interferon-gamma released is usually assayed by an ENZYME-LINKED IMMUNOSORBENT ASSAY.
The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.
Large, phagocytic mononuclear leukocytes produced in the vertebrate BONE MARROW and released into the BLOOD; contain a large, oval or somewhat indented nucleus surrounded by voluminous cytoplasm and numerous organelles.
A CXC chemokine that is induced by GAMMA-INTERFERON and is chemotactic for MONOCYTES and T-LYMPHOCYTES. It has specificity for the CXCR3 RECEPTOR.
A multimeric complex that functions as a ligand-dependent transcription factor. ISGF3 is assembled in the CYTOPLASM and translocated to the CELL NUCLEUS in response to INTERFERON signaling. It consists of ISGF3-GAMMA and ISGF3-ALPHA, and it regulates expression of many interferon-responsive GENES.
INFLAMMATION of the LIVER in humans that is caused by HEPATITIS C VIRUS lasting six months or more. Chronic hepatitis C can lead to LIVER CIRRHOSIS.
The capacity of a normal organism to remain unaffected by microorganisms and their toxins. It results from the presence of naturally occurring ANTI-INFECTIVE AGENTS, constitutional factors such as BODY TEMPERATURE and immediate acting immune cells such as NATURAL KILLER CELLS.
The biosynthesis of RNA carried out on a template of DNA. The biosynthesis of DNA from an RNA template is called REVERSE TRANSCRIPTION.
A cultured line of C3H mouse FIBROBLASTS that do not adhere to one another and do not express CADHERINS.
The type species of VESICULOVIRUS causing a disease symptomatically similar to FOOT-AND-MOUTH DISEASE in cattle, horses, and pigs. It may be transmitted to other species including humans, where it causes influenza-like symptoms.
An enzyme that catalyzes the conversion of ATP into a series of (2'-5') linked oligoadenylates and pyrophosphate in the presence of double-stranded RNA. These oligonucleotides activate an endoribonuclease (RNase L) which cleaves single-stranded RNA. Interferons can act as inducers of these reactions. EC 2.7.7.-.
Antibodies produced by a single clone of cells.
The process of altering the morphology and functional activity of macrophages so that they become avidly phagocytic. It is initiated by lymphokines, such as the macrophage activation factor (MAF) and the macrophage migration-inhibitory factor (MMIF), immune complexes, C3b, and various peptides, polysaccharides, and immunologic adjuvants.
A critical subpopulation of regulatory T-lymphocytes involved in MHC Class I-restricted interactions. They include both cytotoxic T-lymphocytes (T-LYMPHOCYTES, CYTOTOXIC) and CD8+ suppressor T-lymphocytes.
Manifestations of the immune response which are mediated by antigen-sensitized T-lymphocytes via lymphokines or direct cytotoxicity. This takes place in the absence of circulating antibody or where antibody plays a subordinate role.
Proteins which bind to DNA. The family includes proteins which bind to both double- and single-stranded DNA and also includes specific DNA binding proteins in serum which can be used as markers for malignant diseases.
Elements of limited time intervals, contributing to particular results or situations.
Cell surface molecules on cells of the immune system that specifically bind surface molecules or messenger molecules and trigger changes in the behavior of cells. Although these receptors were first identified in the immune system, many have important functions elsewhere.
A classification of T-lymphocytes, especially into helper/inducer, suppressor/effector, and cytotoxic subsets, based on structurally or functionally different populations of cells.
The rate dynamics in chemical or physical systems.
The process of intracellular viral multiplication, consisting of the synthesis of PROTEINS; NUCLEIC ACIDS; and sometimes LIPIDS, and their assembly into a new infectious particle.
A soluble factor produced by MONOCYTES; MACROPHAGES, and other cells which activates T-lymphocytes and potentiates their response to mitogens or antigens. Interleukin-1 is a general term refers to either of the two distinct proteins, INTERLEUKIN-1ALPHA and INTERLEUKIN-1BETA. The biological effects of IL-1 include the ability to replace macrophage requirements for T-cell activation.
Cells grown in vitro from neoplastic tissue. If they can be established as a TUMOR CELL LINE, they can be propagated in cell culture indefinitely.
Differentiation antigens residing on mammalian leukocytes. CD stands for cluster of differentiation, which refers to groups of monoclonal antibodies that show similar reactivity with certain subpopulations of antigens of a particular lineage or differentiation stage. The subpopulations of antigens are also known by the same CD designation.
An immunoassay utilizing an antibody labeled with an enzyme marker such as horseradish peroxidase. While either the enzyme or the antibody is bound to an immunosorbent substrate, they both retain their biologic activity; the change in enzyme activity as a result of the enzyme-antibody-antigen reaction is proportional to the concentration of the antigen and can be measured spectrophotometrically or with the naked eye. Many variations of the method have been developed.
Connective tissue cells which secrete an extracellular matrix rich in collagen and other macromolecules.
A variation of the PCR technique in which cDNA is made from RNA via reverse transcription. The resultant cDNA is then amplified using standard PCR protocols.
The phenomenon of target cell destruction by immunologically active effector cells. It may be brought about directly by sensitized T-lymphocytes or by lymphoid or myeloid "killer" cells, or it may be mediated by cytotoxic antibody, cytotoxic factor released by lymphoid cells, or complement.
The dormant form of TUBERCULOSIS where the person shows no obvious symptoms and no sign of the causative agent (Mycobacterium tuberculosis) in the SPUTUM despite being positive for tuberculosis infection skin test.
The relationship between the dose of an administered drug and the response of the organism to the drug.
A cytokine that promotes differentiation and activation of EOSINOPHILS. It also triggers activated B-LYMPHOCYTES to differentiate into IMMUNOGLOBULIN-secreting cells.
Any of the infectious diseases of man and other animals caused by species of MYCOBACTERIUM.
Diffusible gene products that act on homologous or heterologous molecules of viral or cellular DNA to regulate the expression of proteins.
Polymers of ETHYLENE OXIDE and water, and their ethers. They vary in consistency from liquid to solid depending on the molecular weight indicated by a number following the name. They are used as SURFACTANTS, dispersing agents, solvents, ointment and suppository bases, vehicles, and tablet excipients. Some specific groups are NONOXYNOLS, OCTOXYNOLS, and POLOXAMERS.
Genetically identical individuals developed from brother and sister matings which have been carried out for twenty or more generations, or by parent x offspring matings carried out with certain restrictions. All animals within an inbred strain trace back to a common ancestor in the twentieth generation.
Specialized cells of the hematopoietic system that have branch-like extensions. They are found throughout the lymphatic system, and in non-lymphoid tissues such as SKIN and the epithelia of the intestinal, respiratory, and reproductive tracts. They trap and process ANTIGENS, and present them to T-CELLS, thereby stimulating CELL-MEDIATED IMMUNITY. They are different from the non-hematopoietic FOLLICULAR DENDRITIC CELLS, which have a similar morphology and immune system function, but with respect to humoral immunity (ANTIBODY PRODUCTION).
One of several skin tests to determine past or present tuberculosis infection. A purified protein derivative of the tubercle bacilli, called tuberculin, is introduced into the skin by scratch, puncture, or interdermal injection.
A cytokine that stimulates the growth and differentiation of B-LYMPHOCYTES and is also a growth factor for HYBRIDOMAS and plasmacytomas. It is produced by many different cells including T-LYMPHOCYTES; MONOCYTES; and FIBROBLASTS.
The fission of a CELL. It includes CYTOKINESIS, when the CYTOPLASM of a cell is divided, and CELL NUCLEUS DIVISION.
DNA sequences which are recognized (directly or indirectly) and bound by a DNA-dependent RNA polymerase during the initiation of transcription. Highly conserved sequences within the promoter include the Pribnow box in bacteria and the TATA BOX in eukaryotes.
Subset of helper-inducer T-lymphocytes which synthesize and secrete the interleukins IL-4, IL-5, IL-6, and IL-10. These cytokines influence B-cell development and antibody production as well as augmenting humoral responses.
Endogenous substances, usually proteins, which are effective in the initiation, stimulation, or termination of the genetic transcription process.
Subpopulation of CD4+ lymphocytes that cooperate with other lymphocytes (either T or B) to initiate a variety of immune functions. For example, helper-inducer T-cells cooperate with B-cells to produce antibodies to thymus-dependent antigens and with other subpopulations of T-cells to initiate a variety of cell-mediated immune functions.
The type species of CARDIOVIRUS causing encephalomyelitis and myocarditis in rodents, pigs, and monkeys. Infection in man has been reported with CNS involvement but without myocarditis.
White blood cells formed in the body's lymphoid tissue. The nucleus is round or ovoid with coarse, irregularly clumped chromatin while the cytoplasm is typically pale blue with azurophilic (if any) granules. Most lymphocytes can be classified as either T or B (with subpopulations of each), or NATURAL KILLER CELLS.
A species of gram-positive, aerobic bacteria that produces TUBERCULOSIS in humans, other primates, CATTLE; DOGS; and some other animals which have contact with humans. Growth tends to be in serpentine, cordlike masses in which the bacilli show a parallel orientation.
A Janus kinase subtype that is involved in signaling from a broad variety of CYTOKINE RECEPTORS.
Glycoproteins found on the membrane or surface of cells.
An interferon regulatory factor that recruits STAT1 PROTEIN and STAT2 PROTEIN heterodimers to interferon-stimulated response elements and functions as an immediate-early protein.
A signal transducer and activator of transcription that mediates cellular responses to TYPE I INTERFERONS. Stat2 protein is associated constitutively with INTERFERON REGULATORY FACTOR-9. After PHOSPHORYLATION Stat2 forms the IFN-STIMULATED GENE FACTOR 3 COMPLEX to regulate expression of target GENES.
Progressive restriction of the developmental potential and increasing specialization of function that leads to the formation of specialized cells, tissues, and organs.
Complex of at least five membrane-bound polypeptides in mature T-lymphocytes that are non-covalently associated with one another and with the T-cell receptor (RECEPTORS, ANTIGEN, T-CELL). The CD3 complex includes the gamma, delta, epsilon, zeta, and eta chains (subunits). When antigen binds to the T-cell receptor, the CD3 complex transduces the activating signals to the cytoplasm of the T-cell. The CD3 gamma and delta chains (subunits) are separate from and not related to the gamma/delta chains of the T-cell receptor (RECEPTORS, ANTIGEN, T-CELL, GAMMA-DELTA).
An INTEFERON-inducible CXC chemokine that is specific for the CXCR3 RECEPTOR.
Substances that augment, stimulate, activate, potentiate, or modulate the immune response at either the cellular or humoral level. The classical agents (Freund's adjuvant, BCG, Corynebacterium parvum, et al.) contain bacterial antigens. Some are endogenous (e.g., histamine, interferon, transfer factor, tuftsin, interleukin-1). Their mode of action is either non-specific, resulting in increased immune responsiveness to a wide variety of antigens, or antigen-specific, i.e., affecting a restricted type of immune response to a narrow group of antigens. The therapeutic efficacy of many biological response modifiers is related to their antigen-specific immunoadjuvanticity.
Lymphoid cells concerned with humoral immunity. They are short-lived cells resembling bursa-derived lymphocytes of birds in their production of immunoglobulin upon appropriate stimulation.
Large, transmembrane, non-covalently linked glycoproteins (alpha and beta). Both chains can be polymorphic although there is more structural variation in the beta chains. The class II antigens in humans are called HLA-D ANTIGENS and are coded by a gene on chromosome 6. In mice, two genes named IA and IE on chromosome 17 code for the H-2 antigens. The antigens are found on B-lymphocytes, macrophages, epidermal cells, and sperm and are thought to mediate the competence of and cellular cooperation in the immune response. The term IA antigens used to refer only to the proteins encoded by the IA genes in the mouse, but is now used as a generic term for any class II histocompatibility antigen.
The uptake of naked or purified DNA by CELLS, usually meaning the process as it occurs in eukaryotic cells. It is analogous to bacterial transformation (TRANSFORMATION, BACTERIAL) and both are routinely employed in GENE TRANSFER TECHNIQUES.
A CXC chemokine that is induced by GAMMA-INTERFERON. It is a chemotactic factor for activated T-LYMPHOCYTES and has specificity for the CXCR3 RECEPTOR.
The major immunoglobulin isotype class in normal human serum. There are several isotype subclasses of IgG, for example, IgG1, IgG2A, and IgG2B.
In vitro method for producing large amounts of specific DNA or RNA fragments of defined length and sequence from small amounts of short oligonucleotide flanking sequences (primers). The essential steps include thermal denaturation of the double-stranded target molecules, annealing of the primers to their complementary sequences, and extension of the annealed primers by enzymatic synthesis with DNA polymerase. The reaction is efficient, specific, and extremely sensitive. Uses for the reaction include disease diagnosis, detection of difficult-to-isolate pathogens, mutation analysis, genetic testing, DNA sequencing, and analyzing evolutionary relationships.
White blood cells. These include granular leukocytes (BASOPHILS; EOSINOPHILS; and NEUTROPHILS) as well as non-granular leukocytes (LYMPHOCYTES and MONOCYTES).
A positive regulatory effect on physiological processes at the molecular, cellular, or systemic level. At the molecular level, the major regulatory sites include membrane receptors, genes (GENE EXPRESSION REGULATION), mRNAs (RNA, MESSENGER), and proteins.
The introduction of a phosphoryl group into a compound through the formation of an ester bond between the compound and a phosphorus moiety.
A cytokine which resembles IL-1 structurally and IL-12 functionally. It enhances the cytotoxic activity of NK CELLS and CYTOTOXIC T-LYMPHOCYTES, and appears to play a role both as neuroimmunomodulator and in the induction of mucosal immunity.
Naturally occurring or experimentally induced animal diseases with pathological processes sufficiently similar to those of human diseases. They are used as study models for human diseases.
A genus of FLAVIVIRIDAE causing parenterally-transmitted HEPATITIS C which is associated with transfusions and drug abuse. Hepatitis C virus is the type species.
A large lobed glandular organ in the abdomen of vertebrates that is responsible for detoxification, metabolism, synthesis and storage of various substances.
Interferon-induced DYNAMIN-like GTP-binding proteins localized in the cytoplasm, nuclear pore complex and nucleus. They play a role in antiviral defense and immunity.
Molecules found on the surface of some, but not all, B-lymphocytes, T-lymphocytes, and macrophages, which recognize and combine with the Fc (crystallizable) portion of immunoglobulin molecules.
Either of the pair of organs occupying the cavity of the thorax that effect the aeration of the blood.
The process in which substances, either endogenous or exogenous, bind to proteins, peptides, enzymes, protein precursors, or allied compounds. Specific protein-binding measures are often used as assays in diagnostic assessments.
An increase in the rate of synthesis of an enzyme due to the presence of an inducer which acts to derepress the gene responsible for enzyme synthesis.
Genetic loci in the vertebrate major histocompatibility complex that encode polymorphic products which control the immune response to specific antigens. The genes are found in the HLA-D region in humans and in the I region in mice.
Detection of RNA that has been electrophoretically separated and immobilized by blotting on nitrocellulose or other type of paper or nylon membrane followed by hybridization with labeled NUCLEIC ACID PROBES.
Immunized T-lymphocytes which can directly destroy appropriate target cells. These cytotoxic lymphocytes may be generated in vitro in mixed lymphocyte cultures (MLC), in vivo during a graft-versus-host (GVH) reaction, or after immunization with an allograft, tumor cell or virally transformed or chemically modified target cell. The lytic phenomenon is sometimes referred to as cell-mediated lympholysis (CML). These CD8-positive cells are distinct from NATURAL KILLER CELLS and NATURAL KILLER T-CELLS. There are two effector phenotypes: TC1 and TC2.
The genetic region which contains the loci of genes which determine the structure of the serologically defined (SD) and lymphocyte-defined (LD) TRANSPLANTATION ANTIGENS, genes which control the structure of the IMMUNE RESPONSE-ASSOCIATED ANTIGENS, HUMAN; the IMMUNE RESPONSE GENES which control the ability of an animal to respond immunologically to antigenic stimuli, and genes which determine the structure and/or level of the first four components of complement.
Short sequences (generally about 10 base pairs) of DNA that are complementary to sequences of messenger RNA and allow reverse transcriptases to start copying the adjacent sequences of mRNA. Primers are used extensively in genetic and molecular biology techniques.
A group of genetically identical cells all descended from a single common ancestral cell by mitosis in eukaryotes or by binary fission in prokaryotes. Clone cells also include populations of recombinant DNA molecules all carrying the same inserted sequence. (From King & Stansfield, Dictionary of Genetics, 4th ed)
A deoxyribonucleotide polymer that is the primary genetic material of all cells. Eukaryotic and prokaryotic organisms normally contain DNA in a double-stranded state, yet several important biological processes transiently involve single-stranded regions. DNA, which consists of a polysugar-phosphate backbone possessing projections of purines (adenine and guanine) and pyrimidines (thymine and cytosine), forms a double helix that is held together by hydrogen bonds between these purines and pyrimidines (adenine to thymine and guanine to cytosine).
The action of a drug in promoting or enhancing the effectiveness of another drug.
An NADPH-dependent enzyme that catalyzes the conversion of L-ARGININE and OXYGEN to produce CITRULLINE and NITRIC OXIDE.
A heterogeneous group of immunocompetent cells that mediate the cellular immune response by processing and presenting antigens to the T-cells. Traditional antigen-presenting cells include MACROPHAGES; DENDRITIC CELLS; LANGERHANS CELLS; and B-LYMPHOCYTES. FOLLICULAR DENDRITIC CELLS are not traditional antigen-presenting cells, but because they hold antigen on their cell surface in the form of IMMUNE COMPLEXES for B-cell recognition they are considered so by some authors.
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
Differentiation antigens found on thymocytes and on cytotoxic and suppressor T-lymphocytes. CD8 antigens are members of the immunoglobulin supergene family and are associative recognition elements in MHC (Major Histocompatibility Complex) Class I-restricted interactions.
Domesticated bovine animals of the genus Bos, usually kept on a farm or ranch and used for the production of meat or dairy products or for heavy labor.
The bovine variety of the tubercle bacillus. It is called also Mycobacterium tuberculosis var. bovis.
Substances elaborated by bacteria that have antigenic activity.
Laboratory mice that have been produced from a genetically manipulated EGG or EMBRYO, MAMMALIAN.
Group of chemokines with paired cysteines separated by a different amino acid. CXC chemokines are chemoattractants for neutrophils but not monocytes.
Process of classifying cells of the immune system based on structural and functional differences. The process is commonly used to analyze and sort T-lymphocytes into subsets based on CD antigens by the technique of flow cytometry.
A subclass of HLA-D antigens that consist of alpha and beta chains. The inheritance of HLA-DR antigens differs from that of the HLA-DQ ANTIGENS and HLA-DP ANTIGENS.
Immunoglobulin molecules having a specific amino acid sequence by virtue of which they interact only with the ANTIGEN (or a very similar shape) that induced their synthesis in cells of the lymphoid series (especially PLASMA CELLS).
Antigens expressed on the cell membrane of T-lymphocytes during differentiation, activation, and normal and neoplastic transformation. Their phenotypic characterization is important in differential diagnosis and studies of thymic ontogeny and T-cell function.
One of the mechanisms by which CELL DEATH occurs (compare with NECROSIS and AUTOPHAGOCYTOSIS). Apoptosis is the mechanism responsible for the physiological deletion of cells and appears to be intrinsically programmed. It is characterized by distinctive morphologic changes in the nucleus and cytoplasm, chromatin cleavage at regularly spaced sites, and the endonucleolytic cleavage of genomic DNA; (DNA FRAGMENTATION); at internucleosomal sites. This mode of cell death serves as a balance to mitosis in regulating the size of animal tissues and in mediating pathologic processes associated with tumor growth.
A pathological process characterized by injury or destruction of tissues caused by a variety of cytologic and chemical reactions. It is usually manifested by typical signs of pain, heat, redness, swelling, and loss of function.
Forceful administration under the skin of liquid medication, nutrient, or other fluid through a hollow needle piercing the skin.
A group of deoxyribonucleotides (up to 12) in which the phosphate residues of each deoxyribonucleotide act as bridges in forming diester linkages between the deoxyribose moieties.
Membrane glycoproteins consisting of an alpha subunit and a BETA 2-MICROGLOBULIN beta subunit. In humans, highly polymorphic genes on CHROMOSOME 6 encode the alpha subunits of class I antigens and play an important role in determining the serological specificity of the surface antigen. Class I antigens are found on most nucleated cells and are generally detected by their reactivity with alloantisera. These antigens are recognized during GRAFT REJECTION and restrict cell-mediated lysis of virus-infected cells.
The insertion of recombinant DNA molecules from prokaryotic and/or eukaryotic sources into a replicating vehicle, such as a plasmid or virus vector, and the introduction of the resultant hybrid molecules into recipient cells without altering the viability of those cells.
A species of RESPIROVIRUS also called hemadsorption virus 2 (HA2), which causes laryngotracheitis in humans, especially children.
RNA consisting of two strands as opposed to the more prevalent single-stranded RNA. Most of the double-stranded segments are formed from transcription of DNA by intramolecular base-pairing of inverted complementary sequences separated by a single-stranded loop. Some double-stranded segments of RNA are normal in all organisms.
A pteridine derivative present in body fluids; elevated levels result from immune system activation, malignant disease, allograft rejection, and viral infections. (From Stedman, 26th ed) Neopterin also serves as a precursor in the biosynthesis of biopterin.
Molecules on the surface of T-lymphocytes that recognize and combine with antigens. The receptors are non-covalently associated with a complex of several polypeptides collectively called CD3 antigens (ANTIGENS, CD3). Recognition of foreign antigen and the major histocompatibility complex is accomplished by a single heterodimeric antigen-receptor structure, composed of either alpha-beta (RECEPTORS, ANTIGEN, T-CELL, ALPHA-BETA) or gamma-delta (RECEPTORS, ANTIGEN, T-CELL, GAMMA-DELTA) chains.
Antibiotic substance isolated from streptomycin-producing strains of Streptomyces griseus. It acts by inhibiting elongation during protein synthesis.
A CALCIUM-independent subtype of nitric oxide synthase that may play a role in immune function. It is an inducible enzyme whose expression is transcriptionally regulated by a variety of CYTOKINES.
The outward appearance of the individual. It is the product of interactions between genes, and between the GENOTYPE and the environment.
INFLAMMATION of the LIVER in humans caused by HEPATITIS C VIRUS, a single-stranded RNA virus. Its incubation period is 30-90 days. Hepatitis C is transmitted primarily by contaminated blood parenterally, and is often associated with transfusion and intravenous drug abuse. However, in a significant number of cases, the source of hepatitis C infection is unknown.
The first continuously cultured human malignant CELL LINE, derived from the cervical carcinoma of Henrietta Lacks. These cells are used for VIRUS CULTIVATION and antitumor drug screening assays.
The sum of the weight of all the atoms in a molecule.
Antigens on surfaces of cells, including infectious or foreign cells or viruses. They are usually protein-containing groups on cell membranes or walls and may be isolated.
Any process by which toxicity, metabolism, absorption, elimination, preferred route of administration, safe dosage range, etc., for a drug or group of drugs is determined through clinical assessment in humans or veterinary animals.
A free radical gas produced endogenously by a variety of mammalian cells, synthesized from ARGININE by NITRIC OXIDE SYNTHASE. Nitric oxide is one of the ENDOTHELIUM-DEPENDENT RELAXING FACTORS released by the vascular endothelium and mediates VASODILATION. It also inhibits platelet aggregation, induces disaggregation of aggregated platelets, and inhibits platelet adhesion to the vascular endothelium. Nitric oxide activates cytosolic GUANYLATE CYCLASE and thus elevates intracellular levels of CYCLIC GMP.
The specific failure of a normally responsive individual to make an immune response to a known antigen. It results from previous contact with the antigen by an immunologically immature individual (fetus or neonate) or by an adult exposed to extreme high-dose or low-dose antigen, or by exposure to radiation, antimetabolites, antilymphocytic serum, etc.
Therapy with two or more separate preparations given for a combined effect.
A protein extracted from boiled culture of tubercle bacilli (MYCOBACTERIUM TUBERCULOSIS). It is used in the tuberculin skin test (TUBERCULIN TEST) for the diagnosis of tuberculosis infection in asymptomatic persons.
A phenomenon in which infection by a first virus results in resistance of cells or tissues to infection by a second, unrelated virus.
A class of enzymes that catalyze oxidation-reduction reactions of amino acids.
Linear POLYPEPTIDES that are synthesized on RIBOSOMES and may be further modified, crosslinked, cleaved, or assembled into complex proteins with several subunits. The specific sequence of AMINO ACIDS determines the shape the polypeptide will take, during PROTEIN FOLDING, and the function of the protein.
A negative regulatory effect on physiological processes at the molecular, cellular, or systemic level. At the molecular level, the major regulatory sites include membrane receptors, genes (GENE EXPRESSION REGULATION), mRNAs (RNA, MESSENGER), and proteins.
Deliberate stimulation of the host's immune response. ACTIVE IMMUNIZATION involves administration of ANTIGENS or IMMUNOLOGIC ADJUVANTS. PASSIVE IMMUNIZATION involves administration of IMMUNE SERA or LYMPHOCYTES or their extracts (e.g., transfer factor, immune RNA) or transplantation of immunocompetent cell producing tissue (thymus or bone marrow).
The parts of a macromolecule that directly participate in its specific combination with another molecule.
Compounds and molecular complexes that consist of very large numbers of atoms and are generally over 500 kDa in size. In biological systems macromolecular substances usually can be visualized using ELECTRON MICROSCOPY and are distinguished from ORGANELLES by the lack of a membrane structure.
A malignant neoplasm derived from cells that are capable of forming melanin, which may occur in the skin of any part of the body, in the eye, or, rarely, in the mucous membranes of the genitalia, anus, oral cavity, or other sites. It occurs mostly in adults and may originate de novo or from a pigmented nevus or malignant lentigo. Melanomas frequently metastasize widely, and the regional lymph nodes, liver, lungs, and brain are likely to be involved. The incidence of malignant skin melanomas is rising rapidly in all parts of the world. (Stedman, 25th ed; from Rook et al., Textbook of Dermatology, 4th ed, p2445)
Ribonucleic acid that makes up the genetic material of viruses.
A classification of lymphocytes based on structurally or functionally different populations of cells.
The quantity of measurable virus in a body fluid. Change in viral load, measured in plasma, is sometimes used as a SURROGATE MARKER in disease progression.
A proinflammatory cytokine produced primarily by T-LYMPHOCYTES or their precursors. Several subtypes of interleukin-17 have been identified, each of which is a product of a unique gene.
Extrachromosomal, usually CIRCULAR DNA molecules that are self-replicating and transferable from one organism to another. They are found in a variety of bacterial, archaeal, fungal, algal, and plant species. They are used in GENETIC ENGINEERING as CLONING VECTORS.
Substances that are recognized by the immune system and induce an immune reaction.
A general term for diseases produced by viruses.
An acidic glycoprotein of MW 23 kDa with internal disulfide bonds. The protein is produced in response to a number of inflammatory mediators by mesenchymal cells present in the hemopoietic environment and at peripheral sites of inflammation. GM-CSF is able to stimulate the production of neutrophilic granulocytes, macrophages, and mixed granulocyte-macrophage colonies from bone marrow cells and can stimulate the formation of eosinophil colonies from fetal liver progenitor cells. GM-CSF can also stimulate some functional activities in mature granulocytes and macrophages.
Evaluation undertaken to assess the results or consequences of management and procedures used in combating disease in order to determine the efficacy, effectiveness, safety, and practicability of these interventions in individual cases or series.
A parasitic hemoflagellate of the subgenus Leishmania leishmania that infects man and animals and causes cutaneous leishmaniasis (LEISHMANIASIS, CUTANEOUS) of the Old World. Transmission is by Phlebotomus sandflies.
Cell surface receptors for INTERLEUKIN-10. They exist as a tetramer of two alpha chains (INTERLEUKIN-10 RECEPTOR ALPHA CHAIN) and two beta chains (INTERLEUKIN-10 RECEPTOR, BETA CHAIN). Signaling from interleukin-10 receptors occurs through their interaction with JANUS KINASES.
MYCOBACTERIUM infections of the lung.
Lining of the INTESTINES, consisting of an inner EPITHELIUM, a middle LAMINA PROPRIA, and an outer MUSCULARIS MUCOSAE. In the SMALL INTESTINE, the mucosa is characterized by a series of folds and abundance of absorptive cells (ENTEROCYTES) with MICROVILLI.
Recombinant proteins produced by the GENETIC TRANSLATION of fused genes formed by the combination of NUCLEIC ACID REGULATORY SEQUENCES of one or more genes with the protein coding sequences of one or more genes.
Histochemical localization of immunoreactive substances using labeled antibodies as reagents.
A signal transducer and activator of transcription that mediates cellular responses to INTERLEUKIN-12 in T-LYMPHOCYTES. Stat4 is an important signaling molecule for differentiation in TH1 CELLS.
An active immunizing agent and a viable avirulent attenuated strain of Mycobacterium tuberculosis, var. bovis, which confers immunity to mycobacterial infections. It is used also in immunotherapy of neoplasms due to its stimulation of antibodies and non-specific immunity.
Electrophoresis in which a polyacrylamide gel is used as the diffusion medium.
A cytokine synthesized by T-LYMPHOCYTES that produces proliferation, immunoglobulin isotype switching, and immunoglobulin production by immature B-LYMPHOCYTES. It appears to play a role in regulating inflammatory and immune responses.
Tuberculosis of the serous membrane lining the thoracic cavity and surrounding the lungs.
Infections with bacteria of the genus LISTERIA.
A constitution or condition of the body which makes the tissues react in special ways to certain extrinsic stimuli and thus tends to make the individual more than usually susceptible to certain diseases.
Proteins found in any species of virus.
They are oval or bean shaped bodies (1 - 30 mm in diameter) located along the lymphatic system.
A family of structurally related proteins that are induced by CYTOKINES and negatively regulate cytokine-mediated SIGNAL TRANSDUCTION PATHWAYS. SOCS proteins contain a central SH2 DOMAIN and a C-terminal region of homology known as the SOCS box.
The biosynthesis of PEPTIDES and PROTEINS on RIBOSOMES, directed by MESSENGER RNA, via TRANSFER RNA that is charged with standard proteinogenic AMINO ACIDS.
The type species of RESPIROVIRUS in the subfamily PARAMYXOVIRINAE. It is the murine version of HUMAN PARAINFLUENZA VIRUS 1, distinguished by host range.
A defect of leukocyte function in which phagocytic cells ingest but fail to digest bacteria, resulting in recurring bacterial infections with granuloma formation. When chronic granulomatous disease is caused by mutations in the CYBB gene, the condition is inherited in an X-linked recessive pattern. When chronic granulomatous disease is caused by CYBA, NCF1, NCF2, or NCF4 gene mutations, the condition is inherited in an autosomal recessive pattern.

Surfactant protein A suppresses reactive nitrogen intermediates by alveolar macrophages in response to Mycobacterium tuberculosis. (1/21806)

Mycobacterium tuberculosis attaches to, enters, and replicates within alveolar macrophages (AMs). Our previous studies suggest that surfactant protein A (SP-A) can act as a ligand in the attachment of M. tuberculosis to AMs. Reactive nitrogen intermediates (RNIs) play a significant role in the killing of mycobacteria. We have demonstrated that RNI levels generated by AMs were significantly increased when interferon-gamma-primed AMs were incubated with M. tuberculosis. However, the RNI levels were significantly suppressed in the presence of SP-A (10 microg/ml). The specificity of SP-A's effect was demonstrated by the use of F(ab')2 fragments of anti-SP-A monoclonal antibodies and by the use of mannosyl-BSA, which blocked the suppression of RNI levels by SP-A. Furthermore, incubation of deglycosylated SP-A with M. tuberculosis failed to suppress RNI by AMs, suggesting that the oligosaccharide component of SP-A, which binds to M. tuberculosis, is necessary for this effect. These results show that SP-A-mediated binding of M. tuberculosis to AMs significantly decreased RNI levels, suggesting that this may be one mechanism by which M. tuberculosis diminishes the cytotoxic response of activated AMs.  (+info)

Shp-2 tyrosine phosphatase functions as a negative regulator of the interferon-stimulated Jak/STAT pathway. (2/21806)

Shp-2 is an SH2 domain-containing protein tyrosine phosphatase. Although the mechanism remains to be defined, substantial experimental data suggest that Shp-2 is primarily a positive regulator in cell growth and development. We present evidence here that Shp-2, while acting to promote mitogenic signals, also functions as a negative effector in interferon (IFN)-induced growth-inhibitory and apoptotic pathways. Treatment of mouse fibroblast cells lacking a functional Shp-2 with IFN-alpha or IFN-gamma resulted in an augmented suppression of cell viability compared to that of wild-type cells. To dissect the molecular mechanism, we examined IFN-induced activation of signal transducers and activators of transcription (STATs) by electrophoretic mobility shift assay, using a specific DNA probe (hSIE). The amounts of STAT proteins bound to hSIE upon IFN-alpha or IFN-gamma stimulation were significantly increased in Shp-2(-/-) cells. Consistently, tyrosine phosphorylation levels of Stat1 upon IFN-gamma treatment and, to a lesser extent, upon IFN-alpha stimulation were markedly elevated in mutant cells. Furthermore, IFN-gamma induced a higher level of caspase 1 expression in Shp-2(-/-) cells than in wild-type cells. Reintroduction of wild-type Shp-2 protein reversed the hypersensitivity of Shp-2(-/-) fibroblasts to the cytotoxic effect of IFN-alpha and IFN-gamma. Excessive activation of STATs by IFNs was also diminished in mutant cells in which Shp-2 had been reintroduced. Together, these results establish that Shp-2 functions as a negative regulator of the Jak/STAT pathway. We propose that Shp-2 acts to promote cell growth and survival through two mechanisms, i.e., the stimulation of growth factor-initiated mitogenic pathways and the suppression of cytotoxic effect elicited by cytokines, such as IFNs.  (+info)

Systemic administration of rIL-12 synergistically enhances the therapeutic effect of a TNF gene-transduced cancer vaccine. (3/21806)

Interleukin-12 (IL-12) is a potent antitumor cytokine, which induces and enhances the activity of natural killer (NK) cells, lymphokine activated killer (LAK) cells and cytotoxic T lymphocytes (CTL). IL-12 also stimulates IFN-gamma production from both T cells and NK cells. In this study, we transfected methylcholanthrene-induced fibrosarcoma (MCA-D) with TNF gene and investigated the therapeutic effect of TNF gene-transduced cancer vaccine and whether the vaccination effect is enhanced by systemic administration of recombinant IL-12 (rIL-12), in a murine model. TNF gene-transduced cancer vaccine or systemic administration of rIL-12 showed slight or moderate inhibition of pre-established tumor. However, simultaneous application of the vaccine and rIL-12 resulted in complete eradication. The cytotoxicity of CTL against parental tumor cells was enhanced with the combination of the vaccine and rIL-12, and IFN-gamma production from spleen cells also increased synergistically. Our findings show that synergistic enhancement of CTL activity and IFN-gamma production could play an important role in the antitumor effect of combination therapy using TNF gene-transduced cancer vaccine and rIL-12.  (+info)

Interleukin-18 binding protein: a novel modulator of the Th1 cytokine response. (4/21806)

An interleukin-18 binding protein (IL-18BP) was purified from urine by chromatography on IL-18 beads, sequenced, cloned, and expressed in COS7 cells. IL-18BP abolished IL-18 induction of interferon-gamma (IFNgamma), IL-8, and activation of NF-kappaB in vitro. Administration of IL-18BP to mice abrogated circulating IFNgamma following LPS. Thus, IL-18BP functions as an inhibitor of the early Th1 cytokine response. IL-18BP is constitutively expressed in the spleen, belongs to the immunoglobulin superfamily, and has limited homology to the IL-1 type II receptor. Its gene was localized on human chromosome 11q13, and no exon coding for a transmembrane domain was found in an 8.3 kb genomic sequence. Several Poxviruses encode putative proteins highly homologous to IL-18BP, suggesting that viral products may attenuate IL-18 and interfere with the cytotoxic T cell response.  (+info)

Reciprocal control of T helper cell and dendritic cell differentiation. (5/21806)

It is not known whether subsets of dendritic cells provide different cytokine microenvironments that determine the differentiation of either type-1 T helper (TH1) or TH2 cells. Human monocyte (pDC1)-derived dendritic cells (DC1) were found to induce TH1 differentiation, whereas dendritic cells (DC2) derived from CD4+CD3-CD11c- plasmacytoid cells (pDC2) induced TH2 differentiation by use of a mechanism unaffected by interleukin-4 (IL-4) or IL-12. The TH2 cytokine IL-4 enhanced DC1 maturation and killed pDC2, an effect potentiated by IL-10 but blocked by CD40 ligand and interferon-gamma. Thus, a negative feedback loop from the mature T helper cells may selectively inhibit prolonged TH1 or TH2 responses by regulating survival of the appropriate dendritic cell subset.  (+info)

Generation of CD8(+) T-cell responses to Mycobacterium bovis and mycobacterial antigen in experimental bovine tuberculosis. (6/21806)

Protective immunity against tuberculosis is considered to be essentially cell mediated, and an important role for CD8(+) T lymphocytes has been suggested by several studies of murine and human infections. The present work, using an experimental model of infection with Mycobacterium bovis in cattle, showed that live M. bovis elicits the activation of CD8(+) T cells in vitro. However, a sonic extract prepared from M. bovis (MBSE) and protein purified derivative (PPDb) also induced a considerable degree of activation of the CD8(+) T cells. Analysis of proliferative responses of peripheral blood mononuclear cells, purified CD8(+) T cells, and CD8(+) T-cell clones to M. bovis and to soluble antigenic preparations (MBSE, PPDb) showed that the responses of all three types of cells were always superior for live mycobacteria but that strong responses were also obtained with complex soluble preparations. Furthermore, while cytotoxic capabilities were not investigated, the CD8(+) T cells were found to produce and release gamma interferon in response to antigen (live and soluble), which indicated one possible protective mechanism for these cells in bovine tuberculosis. Finally, it was demonstrated by metabolic inhibition with brefeldin A and cytochalasin D at the clonal level that an endogenous pathway of antigen processing is required for presentation to bovine CD8(+) cells and that presentation is also dependent on phagocytosis of the antigen.  (+info)

Pathogenicity island 2 mutants of Salmonella typhimurium are efficient carriers for heterologous antigens and enable modulation of immune responses. (7/21806)

The potential use as vaccine delivery system of Salmonella typhimurium strains harboring defined mutations in the sseC (HH104) and sseD (MvP101) genes, which encode putative effector proteins of the type III secretion system of Salmonella pathogenicity island 2, was evaluated and compared with that of the well-characterized aroA mutant strain SL7207 by using beta-galactosidase (beta-Gal) as a model antigen. When orally administered to immune-competent or gamma interferon-deficient (IFN-gamma-/-) BALB/c mice, both mutants were found to be highly attenuated (50% lethal dose, >10(9) bacteria). Both strains were also able to efficiently colonize and persist in Peyer's patches. Immunization with HH104 and MvP101 triggered beta-Gal-specific serum and mucosal antibody responses equivalent to or stronger than those observed in SL7207-immunized mice. Although immunoglobulin G2 (IgG2) serum antibodies were dominant in all groups, IgG1 was also significantly increased in mice vaccinated with MvP101 and SL7207. Comparable beta-Gal-specific IgA and IgG antibodies were detected in intestinal lavages from mice immunized with the different strains. Antigen-specific CD4(+) T-helper cells were generated after vaccination with all vaccine prototypes; however, responses were significantly more efficient when HH104 and MvP101 were used (P < 0.05). Significantly higher levels of IFN-gamma were produced by restimulated spleen cells from mice immunized with HH104 than from those vaccinated with the MvP101 or SL7207 derivatives (P +info)

Enhanced Th1 and dampened Th2 responses synergize to inhibit acute granulomatous and fibrotic responses in murine schistosomiasis mansoni. (8/21806)

In murine schistosomiasis mansoni, CD4(+) Th1 and Th2 cells participate in the ovum-induced granulomatous inflammation. Previous studies showed that the interleukin-12 (IL-12)-induced Th1 response strongly suppressed the Th2-cell-mediated pulmonary granuloma development in naive or primed mice. However, liver granulomas were only moderately suppressed in egg-vaccinated, recombinant IL-12 (rIL-12)-treated infected mice. The present study shows that repeated rIL-12 injections given during early granuloma development at 5 to 7 weeks after infection prolonged the Th1 phase and resulted in gamma interferon-mediated suppression of liver granulomas. The timing is crucial: if given at 6 to 8 weeks, during the Th2-dominated phase of florid granuloma growth, the treatment is ineffective. Daily injections of rIL-12 given between 5 and 7.5 weeks during the period of granuloma growth achieved a somewhat-stronger diminution in granuloma growth with less deposition of collagen but caused 60% mortality and liver pathology. In contrast, combined treatment with rIL-12 and anti-IL-4-anti-IL-10 monoclonal antibody (MAb) injections given during the Th2 phase strongly inhibited liver granuloma growth without mortality. The diminished inflammatory response was accompanied by less deposition of collagen in the liver. Moreover, neutralization of endogenous IL-12 by anti-IL-12 MAbs effectively decreased the early Th1 phase (between 5 and 6 weeks after infection) but not the developing Th2 phase (5 to 7 weeks) of granuloma development. These studies indicate that the granulomatous response in infected mice can be manipulated by utilizing the Th1-Th2-subset antagonism with potential salutary results in the amelioration of fibrous pathology.  (+info)

Peroxisome proliferator-activated receptor-gamma activators inhibit IFN-gamma-induced expression of the T cell-active CXC chemokines IP-10, Mig, and I-TAC in human endothelial cells. J Immunol. 2000 Jun 15; 164(12):6503-8 ...
We previously reported that resting mouse peritoneal macrophages (PM) constitutively express low levels of IFN-gamma, whose production is consistently enhanced by exogenous IFN-gamma. In this study, we investigated the effects of IL-12 on the replication of vesicular stomatitis virus and on IFN-gamma gene expression in mouse PM. The addition of IL-12 to freshly explanted PM resulted in the persistence of an antiviral state to vesicular stomatitis virus, while control PM progressively became permissive for virus replication after 3 to 4 days in culture. The IL-12-induced antiviral state was inhibited by Abs to IFN-gamma, suggesting that endogenous IFN-gamma was largely responsible for this antiviral response. Moreover, IL-12 induced a consistent secretion of IFN-gamma, especially in cultured PM. The IL-1 2-induced antiviral state and IFN-gamma production were observed using PM from various strains of mice, including LPS-defective C3H/HeJ, NK-deficient bg/bg, DBA/2, Swiss (CD1), and Swiss nude ...
The tuberculin skin test for diagnosing Mycobacterium tuberculosis infection suffers from antigenic cross-reactivity of purified protein derivative with BCG, resulting in poor specificity in BCG-vaccinated populations. Comparative genomics has identified several genetic regions in M. tuberculosis and M. bovis that are deleted in M. bovis BCG. Proteins encoded in these regions will form the basis of new specific T-cell-based blood tests that do not cross-react with BCG, but only two, early secretory antigen target 6 and culture filtrate protein 10, have been studied in detail in humans. We investigated four novel gene products, encoded by RD2 (Rv1989c) and RD1 (Rv3873, Rv3878, and Rv3879c), that are absent from most or all of the vaccine strains of BCG, respectively. Sixty-seven overlapping peptides were tested in ex vivo gamma interferon enzyme-linked immunospot assays in 49 patients with culture-confirmed tuberculosis and 38 healthy BCG-vaccinated donors. Forty-five percent (95% confidence ...
Sepsis is the leading cause of death in the ICU with an estimated 6 million victims per year worldwide. Although septic shock is traditionally viewed as an excessive systemic inflammatory reaction to invasive microbial pathogens, pharmacological suppression of the innate immune response in sepsis has proved to be unsuccessful. An important reason for this might be that the vast majority of septic patients survive the initial pro-inflammatory hit, but die in the subsequent immunosuppressed state due to secondary/opportunistic infections. This so-called immunoparalysis is increasingly recognized as the overriding immune dysfunction in septic patients. Reversal of sepsis-induced immunoparalysis is therefore a promising adjunctive treatment for patients presenting with septic shock.. It was demonstrated that interferon-gamma (IFN)-gamma can reverse immunoparalysis in vitro and in vivo in animals and in healthy volunteers. Moreover, in a case-series of septic patients interferon-gamma treatment ...
Activated polymorphonuclear leukocytes (PMNL) might be an important source of host tissue damage. PMNL may be primed by various inflammatory mediators for an increased production of reactive oxygen species (ROS). The short-term priming effects of two cytokines, interferon-#gamma (IFN-#gamma) and tumor necrosis factor-ð (TNF-ð), on rat PMNL in whole unfractionated blood were investigated in the present study. Incubation of PMNL with cytokine alone did not induce the detectable ROS production without addition of a second stimulus - phorbol myristate acetate (PMA) or opsonized zymosan particles (OZP). One-hour incubation with TNF-ð, or the mixture of TNF-ð + IFN-#gamma resulted in a dose-dependent enhancement of ROS production in response to PMA. Moreover, the incubation with both IFN-#gamma and TNF-ð caused a significantly higher increase of ROS production than with TNF-ð alone. However, no priming effects of IFN-#gamma on the PMA-induced ROS production were observed. Finally, none of the ...
TY - JOUR. T1 - Enhancement of primary and secondary immune responses by interferon-gamma.. AU - Yilma, Tilahun. AU - Owens, S.. AU - Fennie, E. H.. AU - Anderson, K. P.. PY - 1989. Y1 - 1989. N2 - We have investigated the effects of interferon-gamma (IFN-gamma) administered with G glycoprotein of vesicular stomatitis virus (VSV), on the neutralizing antibody response. Treatment of mice or cattle with recombinant DNA-derived IFN-gamma at the time of primary immunization with G glycoprotein enhanced the secondary virus-neutralizing antibody response that followed a booster administration of the same antigen without IFN-gamma treatment. Enhancement was statistically significant, and occurred at relatively low doses of IFN-gamma in the absence of any additional adjuvants. Cattle treated with IFN-gamma at the time of primary immunization were also more resistant to VSV challenge than those immunized without IFN-gamma treatment. Such treatment in conjunction with primary immunization may ...
Join or renew your annual membership in the International Cytokine & Interferon Society (ICIS) starting at $60 for Academic/Government Members, $250 for Industry Members and only $40 for students & postdocs for a 3 year membership. Academic/Government Members save € 100 on registration fees, Industry Members save €250 on Industry Registration fees and Students/Postdoc Members save € 50 on Student/Postdoc registrations!. Please visit the cytokine society website to join or renew ICIS prior to registering.. ...
For more information about the Society, please contact our Managing Director, Joan Oefner or fill out the contact form.. International Cytokine & Interferon Society (ICIS) ...
For more information about the Society, please contact our Managing Director, Joan Oefner or fill out the contact form.. International Cytokine & Interferon Society (ICIS) ...
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Amoytop Biotech, in collaboration with BioGeneric Pharma, is developing a recombinant human interferon gamma protein drug, for the treatment of an undefined
A biphasic dose-response curve was observed when the IL-1-dependent HTL clone D10 was exposed to IL-1 plus supernatants from some activated T cell clones but not others. The active component that inhibited proliferation at high concentrations of these supernatants appeared to be IFN-gamma based on the following findings: 1) the biphasic pattern of responsiveness correlated with the presence of IFN-gamma in the supernatants; 2) an anti-IFN-gamma mAb augmented the proliferation of D10 cells to these supernatants; 3) rIFN-gamma inhibited profoundly the response of D10 cells stimulated with rIL-1 plus supernatant from activated D10 cells or with rIL-1 plus rIL-4; 4) the response of D10 cells to rIL-1 plus rIL-2 also was inhibited by rIFN-gamma, although to a lesser extent. The proliferation of an additional Th2 clone stimulated with rIL-1 plus rIL-4 or rIL-2 also was inhibited by rIFN-gamma, implicating IFN-gamma as an inhibitory lymphokine for Th2 cells in general. rIFN-gamma did not affect the ...
Interleukin-12 (IL-12) induces differentiation of T helper 1 (Th1) cells, primarily through its ability to prime T cells for high interferon-gamma (IFN-gamma) production. We now report that the presence of IL-12 during the first several days of in vitro clonal expansion in limiting dilution cultures of polyclonally stimulated human peripheral blood CD4+ and CD8+ T cells also induces stable priming for high IL-10 production. This effect was demonstrated with T cells from both healthy donors and HIV+ patients. Priming for IL-4 production, which requires IL-4, was maximum in cultures containing both IL-12 and IL-4. IL-4 modestly inhibited the IL-12-induced priming for IFN-gamma, but almost completely suppressed the priming for IL-10 production. A proportion of the clones generated from memory CD45RO+ cells, but not those generated from naive CD45RO- CD4+ T cells, produced some combinations of IFN-gamma, IL-10, and IL-4 even in the absence of IL-12 and IL-4, suggesting in vivo cytokine priming; ...
PE anti-human IFN-γ Antibody - Interferon-γ is a potent multifunctional cytokine which is secreted primarily by activated NK cells and T cells.
Brilliant Violet 510™ anti-human IFN-γ Antibody - Interferon-γ is a potent multifunctional cytokine which is secreted primarily by activated NK cells and T cells.
EzWay Human IFN-gamma ELISA Kit,K1331121,Cytokine ELISA Kit,EzWay Cytokine ELISA Kit reduces your assay time to 2.5 hours by integrating incubation of sample & …
Specialist distributor of life science reagents in the UK: Human IFN-gamma/Granzyme B/TNF-alpha FluoroSpotPLUS kit (FSP-011009-2) by Mabtech
PubMed comprises more than 30 million citations for biomedical literature from MEDLINE, life science journals, and online books. Citations may include links to full-text content from PubMed Central and publisher web sites.
Hochkeppel, H K. and Ley, M D., Monoclonal antibody against human ifn-gamma. (1982). Subject Strain Bibliography 1982. 2963 ...
Mouse IFN-gamma ELISPOT kit (Black spots, 2-plate (supplied with plates)). The cytokine ELISPOT (Enzyme-Linked ImmunoSPOT) assay is designed to enumerate cytokine-secreting cells in single cell suspensions of lymphoid tissue, central nerve system (CNS) tissue, bone marrow or preparations of peripheral blood mononuclear cells (PBMCs). The assay has the advantage of detecting only activated/memory T cells and has the ability to detect cytokine release in response to antigen by a single cell thereby permitting direct calculation of responder T cell frequencies. The high sensitivity and easy performance, allowing the determination of peptide-reactive T cells without prior in vitro expansion, makes the ELISPOT assay eminently well suited to monitor T cell responses. The higher sensitivity of ELISPOT in comparison to that of ELISA or intracellular staining is due to the plate-bound antibodies directly capturing the cytokine released by the cell before it is diluted in the supernatant, trapped by high-affinity
I just ran across this on the NMSS site and thought that others here would be interested. Researchers Find Clue to Why Women Get MS More Often Than Men An international team of researchers funded in part by the National ...
Monkey IFN-gamma - IL-10 FluoroSpot Kit (2-plate). The dual-color cytokine FluoroSpot assay is a modification of the Enzyme-Linked ImmunoSPOT (ELISPOT) assay and is designed to detect double cytokine-secreting T cells at the single cell level. The assay is based on the use of fluorescent- instead of enzymatic-labeled conjugates. Previous attempts to develop an immunoenzymatic dual-color ELISPOT assay were less successful because of problematic interpretation of mixed color spots. The dual-color FluoroSpot assay, however, provides good discrimination between single and double cytokine-secreting cells and is particularly suited to detect T cell subpopulations with a characteristic cytokine profile. The sensitivity of the assay lends itself to measure very low frequencies of cytokine-secreting T cells (1/300,000).
Recombinant gamma-interferon has activity in chronic myeloid leukemia. American Journal of Clinical Oncology: Cancer Clinical Trials. 1990 ...
One of the most remarkable means by which tumour cells manage to evade recognition and elimination by the immune system is the release of immunosuppressive mediators, such as interleukin (IL)-10 or transforming growth factor-beta (TGF-beta). For antitumour immunotherapies to reach their full potenti …
The regulation of interleukin 6 (IL-6) is dependent on many factors that include numerous stimuli such as lipopolysaccharide (LPS), viruses, and other cytokines. These studies demonstrate the ability of interferon-gamma (IFN-gamma) to significantly enhance IL-6 mRNA and protein production in LPS-sti …
Background Production of interferon (IFN)-gamma is key to efficient anti-tumor immunity. The present study was set out to investigate effects of IFNgamma on the release of the potent pro-angiogenic mediator IL-8 by human A549 lung carcinoma cells. Methods A549 cells were cultured and stimulated with interleukin (IL)-1beta alone or in combination with IFNgamma. IL-8 production by these cells was analyzed with enzyme linked immuno sorbent assay (ELISA). mRNA-expression was analyzed by real-time PCR and RNase protection assay (RPA), respectively. Expression of inhibitor-kappaBalpha, cellular IL-8, and cyclooxygenase-2 was analyzed by Western blot analysis. Results Here we demonstrate that IFNgamma efficiently reduced IL-8 secretion under the influence of IL-1beta. Surprisingly, real-time PCR analysis and RPA revealed that the inhibitory effect of IFNgamma on IL-8 was not associated with significant changes in mRNA levels. These observations concurred with lack of a modulatory activity of IFNgamma ...
Interferon-gamma is a regulatory cytokine secreted by activated Th1 Cells which promotes phagocytosis of internalized bacteria by Macrophages. This cytokine is also thought to play an important role in the development of Giant Cells and Granulomas ...
Gessani S, Belardelli F. IFN-gamma expression in macrophages and its possible biological significance. Cytokine & growth factor reviews 1998;9(2):117-123 ...
Białko mitochondrialne CMPK2 reguluje tworzenie komórek piankowatych wzmocnionych przez IFN alfa, potencjalnie przyczyniając się do przedwczesnej miażdżycy tętnic w SLE Background: Untimely atherosclerosis happens in sufferers with SLE; nonetheless, the mechanisms stay unclear. Each mitochondrial equipment and proinflammatory cytokine interferon alpha (IFN-α) probably contribute to atherogenic processes in SLE. Right here, we discover the roles of…. Read More ...
Supplementary MaterialsSupplementary File. 0.001, Mann-Whitney rank sum test. (preparations: CTL, 4; IFN- (100 ng/mL, 24 h), 4; * 0.001 vs. CTL, unpaired test. Sample images are taken from CA3 (Fig. 3). Open in a separate window Fig. 2. Microglial cytokine release and iNOS expression. Slice cultures were exposed to IFN- or clodronate (100 g/mL) plus IFN- (1,000 ng/mL) (CLOD+IFN-) for 72 h. (membranes/preparations: CTL, 4/4; IFN- (100 ng/mL), 4/4 (IL-6), and 5/4 (TNF-); IFN- BMT-145027 (1,000 ng/mL), 3/3 (IL-6), and 5/4 (TNF-); CLOD+IFN-, 3/3 (IL-6), and 2/2 (TNF-). * 0.01 vs. CTL and CLOD+IFN-, and 0.05 vs. IFN- (100 ng/mL), one-way ANOVA with Tukeys post hoc BMT-145027 test. (preparations: CTL, 2; IFN- (100 ng/mL), 3; IFN- (1,000 ng/mL), 2. * 0.01 IFN- (100 ng/mL) and IFN- (1,000 ng/mL) vs. CTL, * 0.05 IFN- (1,000 ng/mL) vs. IFN- (100 ng/mL), one-way ANOVA with Tukeys post hoc test. ( 0.05 vs. CTL and CLOD+IFN-, one-way ANOVA with Tukeys post hoc test. (and and and slices/preparations: CTL ...
Induction of IFN-γ- and TNF-α-secreting CD8+ T cell subpopulations upon DC priming.PBMC from volunteer CVD4000#65 were co-cultured with DC alone (media), or p
Although interferon-gamma (IFN-) has a critical function in the noncytolytic elimination of several neurotropic viral infections, the signaling response to the cytokine is not characterized in primary... Read More ...
TY - JOUR. T1 - Interleukin-10 and interferon-gamma gene polymorphisms in patients with nasopharyngeal carcinoma. AU - Farhat, K.. AU - Hassen, E.. AU - Gabbouj, S.. AU - Bouaouina, N.. AU - Chouchane, Lotfi. PY - 2008/6. Y1 - 2008/6. N2 - Nasopharyngeal carcinoma (NPC) is a multifactorial disease. Cytokines driving the immune response seem to be disturbed in NPC patients. Since interleukin-10 (IL-10) is known to reduce the production of interferon-gamma (IFN-γ), we supposed that genetic differences in IL-10 and IFN-γ expression could be a mechanism by which NPC cells escape antitumour immune response. As the production of each cytokine is affected by the genetic background, we investigated the possible association between single nucleotide polymorphisms in genes of IL-10 and IFN-γ with NPC. Different IL-10 -1082 G/A and IFN-γ +874 Τ/Α genotypes were determined in 160 patients with nasopharyngeal carcinoma and 197 healthy controls. No association was found either for each SNP studied alone ...
Spleen cells of Mycobacterium lepraemurium-infected mice were cultured on petri dishes coated with mycobacterial antigens, and antigen-reactive cells were isolated. Upon incubation in mitogen- or antigen-free culture medium, these cells released mediators capable of depressing the in vitro proliferative response of normal splenocytes to specific antigen and to concanavalin A and lipopolysaccharide. One of these mediators was identified with gamma interferon (IFN-gamma), mainly on the basis that treatment of supernatants with monoclonal anti-IFN-gamma antibodies markedly reduced the suppressive activity contained therein. Detectable levels of tumor necrosis factor alpha (TNF-alpha) and TNF-beta were present in spleen cell culture supernatants of infected mice. Moreover, low doses of recombinant TNF-alpha and TNF-beta were found to potentiate the suppressive activity of exogenous IFN-gamma. Soluble T-cell receptors beta were also detected in the culture supernatants. The elimination of these ...
Six patients with the acquired immune deficiency syndrome (AIDS) had exacerbations or recurrences of previously quiescent atopic disease when they developed immunodeficiency. Four developed a different atopic illness from that suffered previously. Atopic symptoms developed within three months after the patients developed AIDS or during prodromal illness. Two of the patients were treated with recombinant interferon gamma: both showed a striking improvement in symptoms and cellular immunity. These results indicate that cellular immunity, through interferon gamma, may have a role in regulating atopic disease. ...
Pulmonary aspergillosis which is an important opportunistic infection in neutropenic patients, is usually caused by Aspergillus fumigatus. Since the pathogenesis of disease is not well understood, the main proposed mechanism is thought to be cell-mediated immunity and cytokine response. The aim of this study was to investigate the local production of cytokines in the lung tissues of rats with experimentally developed aspergillosis, by reverse transcriptase-polymerase chain reaction (RT-PCR). A total of 33 Wistar albino type rats were included in the study with the consent of Experimental Animal Ethics Committee. Twenty-five of the rats were infected with A.fumigatus by intratracheal way, while 8 animals were used as controls. The presence of A.fumigatus in the lung tissues of infected rats was confirmed with the use of quantitative culture and histologic staining methods. RNA isolation from the lung tissue samples of both groups were performed by a commercial kit (Qiagen, Germany). After ...
Activated monocytes play an important role in the pathogenesis of inflammatory arthritis. Blood monocytes which enter the inflamed joint become activated upon adherence to extracellular matrix and exposure to a complex inflammatory environment. We have analyzed the mechanism of monocyte activation by soluble factors present in inflammatory synovial fluid (SF). Greater than 75% of inflammatory SFs tested (a total of 22 fluids to date) increased cell surface expression and dramatically increased mRNA levels of monocyte activation markers Fc gamma RI, Fc gamma RIII, and HLA-DRA. This induction was not triggered by adherence, a known activating stimulus, and several lines of evidence showed that induction was not dependent upon interferon gamma (IFN-gamma). Induction was not prevented by neutralizing anti-IFN-gamma antibodies and IFN-gamma was not detected in the SFs using a sensitive enzyme-linked immunosorbent assay. The SFs also were not able to activate the IFN-gamma-activated transcription ...
Both IL-12 and IFN-gamma have been implicated as principal inducers of type 1 immune responses required for the elimination of intracellular pathogens, such as viruses. We examined the in vivo antiviral role of both cytokines during coronavirus-induced hepatitis in a mouse hepatitis virus (MHV) model. The absence of IFN-gamma function ... read more in mice with a targeted disruption of the IFN-gamma R alpha-chain gene (IFN- gamma R -/-) resulted in increased susceptibility to coronaviral hepatitis associated with augmented viral replication and increased hepatocellular injury. The mutant mice showed a type 1 lymphokine response characterized by the normal high IFN-gamma and low IL-4 production. Unlike MHV-infected wild-type mice, however, the mutant IFN- gamma R -/- mice showed no increase in IL-12 p4O gene expression, similar to that in naive animals. IL-12 treatment failed to restore host resistance in IFN-gamma R -/- mice, but significantly protected MHV-susceptible C57BL/6 mice against ...
ab46604 Human Interferon gamma ELISPOT Kit (Reagents for 10 x 96 tests, without plates) 製品データシート サイトカインの検出に有用 アブカムの ELISPOT キット
This study explored the use of interleukin 2 (IL-2) and interferon gamma (IFN-gamma) gene-modified tumor cells as cellular vaccines for the treatment of bladder cancer. The mouse MBT-2 tumor used is an excellent model for human bladder cancer. This carcinogen-induced tumor of bladder origin resembles human bladder cancer in its etiology and histology, and responds to treatment in a manner similar to its human counterpart. Using retroviral vectors, the human IL-2 and mouse IFN-gamma genes were introduced and expressed in MBT-2 cells. The tumor-forming capacity of the cytokine gene-modified MBT-2 cells was significantly impaired, since no tumors formed in mice injected intradermally with either IL-2- or IFN-gamma-secreting cells, using cell doses far exceeding the minimal tumorigenic dose of parental MBT-2 cells. Furthermore, mice that rejected the IL-2- or IFN-gamma-secreting tumor cells became highly resistant to a subsequent challenge with parental MBT-2 cells, but not to 38C13 cells, a B cell ...
Abcam provides general protocols for Human Interferon gamma ELISA Kit (IFNG) (ab108863). Please download our pdf protocol booklet
ab46652 Human Interferon gamma + IL10 FLUOROSPOT Kit 製品データシート サイトカインの検出に有用 アブカムの ELISPOT キット
Interferon-gamma (IFN-gamma) belongs to the type II interferon family and is secreted by activated immune cells-primarily T and NK cells, but also B-cells and APC. INFG exerts its effect on cells by interacting with the specific IFN-gamma receptor (IFNGR). IFNGR consists of two chains, namely IFNGR1 (also known as the IFNGR alpha chain) and IFNGR2 (also known as the IFNGR beta chain). IFNGR1 is the ligand binding receptor and is required but not sufficient for signal transduction, whereas IFNGR2 do not bind IFNG independently but mainly plays a role in IFNG signaling and is generally the limiting factor in IFNG responsiveness. Both IFNGR chains lack intrinsic kinase/phosphatase activity and thus rely on other signaling proteins like Janus-activated kinase 1 (JAK1), JAK2 and Signal transducer and activator of transcription 1 (STAT-1) for signal transduction. IFNGR complex in its resting state is a preformed tetramer and upon IFNG association undergoes a conformational change. This conformational ...
Interferon-gamma (IFN-gamma) belongs to the type II interferon family and is secreted by activated immune cells-primarily T and NK cells, but also B-cells and APC. INFG exerts its effect on cells by interacting with the specific IFN-gamma receptor (IFNGR). IFNGR consists of two chains, namely IFNGR1 (also known as the IFNGR alpha chain) and IFNGR2 (also known as the IFNGR beta chain). IFNGR1 is the ligand binding receptor and is required but not sufficient for signal transduction, whereas IFNGR2 do not bind IFNG independently but mainly plays a role in IFNG signaling and is generally the limiting factor in IFNG responsiveness. Both IFNGR chains lack intrinsic kinase/phosphatase activity and thus rely on other signaling proteins like Janus-activated kinase 1 (JAK1), JAK2 and Signal transducer and activator of transcription 1 (STAT-1) for signal transduction. IFNGR complex in its resting state is a preformed tetramer and upon IFNG association undergoes a conformational change. This conformational ...
Hamster IFN-γ(Interferon gamma) ELISA Kit from ELISA Genie is a pre-coated immunoassay with a sensitivity of 9.375pg/ml and a range of 15.625-1000pg/ml and has been designed to measure Hamster IFN-γ(Interferon gamma) ELISA Kit in serum, plasma & cell culture supernatant samples. The Hamster IFN-γ(Interferon gamma) ELISA Kit accurately measures natural Hamster IFN-γ(Interferon gamma) levels quantified versus standard curves obtained and is based on antibodies raised against a recombinant Hamster IFN-γ(Interferon gamma) peptide.
We compared cytomegalovirus (CMV)-specific interferon-gamma (IFN-gamma), interleukin 2 (IL-2), and CD154 CD4(+) T cell responses of infants to those from chronically infected adults and from children aged 4-5 years. Magnitudes of the responses were similar, although coexpression of IFN-gamma plus CD154 occurred more than coexpression of IFN-gamma plus IL-2 or IL-2 plus CD154. Responses remained constant during infancy, although the proportion of IFN-gamma-producing cells increased from infancy to adulthood. Most responding cells in infants were undifferentiated (i.e., CD27(+)CD28(+)), although IFN-gamma-producing cells were disproportionately CD27(-). By 12 months after diagnosis, viremia was rarely detectable, indicating that CMV was controlled despite the slow development of CMV-specific CD4(+) T cell responses.
BACKGROUND: The interaction between host lymphocytes and endothelial cells on the transplanted organ is believed to play an important role in acute and chronic graft rejection. Trafficking and recruitment of lymphocytes to the site of inflammation is known to be controlled by several cytokines and chemokines. It is unclear whether endothelial cells themselves can be a source of inflammatory chemoattractant molecules on alloimmune induction. METHODS: Using a semiquantitative polymerase chain reaction method, the authors analyzed the expression of chemokine mRNA coding for interferon (IFN)-gamma-induced protein 10 (IP-10) and monokine induced by IFN-gamma (Mig) in a pool of human aortic endothelial cells. Both of these chemokines are known to be induced by IFN-gamma. Endothelial cell-derived chemokine mRNA was assayed at rest, after IFN-gamma activation, and after co-culture with allogeneic peripheral blood mononuclear cells (PBMC) from normal blood donors with and without a monoclonal antibody to IFN
PubMed comprises more than 30 million citations for biomedical literature from MEDLINE, life science journals, and online books. Citations may include links to full-text content from PubMed Central and publisher web sites.
Murrays recent review (1) provides a welcome discussion of the role of interferon-gamma as an immune activator of macrophages against microbial pathogens. A small section of it, however, must respectfully be challenged. Contrary to what Murray suggests, interferon-gamma apparently cannot protect against tubercle bacilli or Mycobacterium avium in humans. Knowledge of this important fact can help terminate an interpolative idea that has been contradicted by well-controlled experimental evidence.. In our several years of research on many variations of an in-vitro model of human tuberculosis infection, using macrophages and virulent tubercle bacilli, we have never found interferon-gamma protective (2). In fact, ...
Abcams Interferon gamma ELISPOT Kit Colorimetric (ab62899) is a highly sensitive cell-based ELISA for the detection of Interferon gamma in stimulated cells.
Our knowledge of the induction of new molecules by IFN-gamma has led to the characterization of IP-10 and the preparation of a monospecific, polyclonal antibody. Using this reagent we have now examined inflammatory states occurring in human skin and used immunocytochemical staining for the expression of both Ia and IP-10 determinants. After evoking a delayed-type response to purified protein derivative of tuberculin (PPD), we noted the presence of IP-10 in dermal macrophages and endothelial cells. Intense staining of the basal layer of epidermal keratinocytes was prominent at 41 h, and by 1 wk the entire epidermis was staining. The comparison of the amount of IP-10 secreted by keratinocytes vs. macrophages, fibroblasts, and endothelial cells revealed that keratinocytes were by far the major producers of this molecule. The expression of Ia occurred in conjunction with IP-10. The injection of rIFN-gamma mimicked many of the features of the PPD response, including the expression of both Ia and ...
Demographic information provided for all subjects who were eligible for the study after all screening procedures were performed. 7 subjects who were found to be ineligible after randomization but prior to administration of treatment (listed as did not meet continuation criteria in participant flow section) are not included in baseline analysis ...
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Engineered insulinoma cell lines may represent an alternative to isolated islets for transplantation therapy of type 1 diabetes. Success of this approach may require development of cell lines that can withstand cytokine-mediated damage. To this end, we have cultured INS-1 insulinoma cells in increasing concentrations of interleukin-1beta (IL-1beta) + gamma-interferon (IFN-gamma), with approximate weekly iterations over an 8-week period. Based on the C,N diphenyl-N-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium+ ++ bromide (MTT) viability assay, the selected cells, termed INS-1res, were 100% viable after 5 days of treatment with 10 ng/ml of IL-1beta. These cells were also 78 +/- 1.2% viable after 5 days of exposure to the combination of 10 ng/ml IL-1beta and 100 U/ml IFN-gamma, whereas parental INS-1 cells treated in the same manner were only 0.3 +/- 0.03% viable. INS-1res cells were also resistant to treatment with supernatants from activated rat peripheral blood mononuclear cells, whereas ...
Interferon gamma-induced protein 10 (IP-10), or CXCL10, is a chemokine secreted by monocytes, endothelial cells and fibroblasts in response to interferon gamma (IFN-ɣ).
Results IFN-λ was detected in 76 (29,1%) patients and IFN-α in 115 (44%). In 108 patients (41.4%) neither of the cytokines was detected. Both IFN-λ and IFN-α were detected more often and in higher levels in patients. IFN-λ levels did not correlate to the levels of IFN-α, and only in 38 (14.5%) patients had detectable levels of both. Levels of IL-17, IL-23 and IP-10 correlated with each other and with IFN-λ. In comparison, only a weak correlation between levels of IFN-α and IL-23 was observed. Detectable levels of IFN-λ were associated with lower incidence of fever, photosensitivity and also arthritis. Patients with detectable IFN-α had active mucocutaneous disease, serositis and lower levels of C3 and C4, and also presence of anti-Ro/SSA and anti-La/SSB. Incidence of nefritis and DVT was lower in this group. Co-detection of IFN-α and IFN-λ was significantly associated with lymphadenopathy, serositis, cortical dysfunction and presence of antiphospolipid antibodies. IFN-α was ...
TY - JOUR. T1 - Failure to suppress the expansion of the activated CD4 T cell population in interferon γ-deficient mice leads to exacerbation of experimental autoimnaune encephalomyelitis. AU - Chu, Cong Qiu. AU - Wittmer, Susan. AU - Dalton, Dyana K.. PY - 2000/7/3. Y1 - 2000/7/3. N2 - Mice deficient in interferon (IFN)-γ or IFN-γ receptor develop progressive and fatal experimental autoimmune encephalomyelitis (EAE). We demonstrate that CD4 T cells lacking IFN-γ production were required to passively transfer EAE, indicating that they were disease-mediating cells in IFN-γ knockout (KO) mice. IFN-γ KO mice accumulated 10-16-fold more activated CD4 T cells (CD4+CD44(hi)) than wild-type mice in the central nervous system during EAE. CD4+CD44(hi) T cells in the spleen and central nervous system of IFN-γ KO mice during EAE showed markedly increased in vivo proliferation and significantly decreased ex vivo apoptosis compared with those of wild-type mice. IFN-ψ KO CD4+CD44(hi) T cells ...
interferon gamma receptor: distinct from the receptors for interferons alpha and beta; RefSeq NM_000416 (human), NM_010511 (mouse)
Author: Praefcke, G. J. K. et al.; Genre: Other; Published in Print: 2000; Title: Identification of regions in human guanylate binding protein 1 (hGBP1) responsible for oligomerization and GTPase activity
This kit is ideal for users who want a convenient and sensitive assay. The assay is designed for the enumeration of cells secreting human IFN-γ. The kit includes ELISpot 8-well strip plates pre-coated with monoclonal antibody, biotinylated detection antibody, Streptavidin-HRP and ready-to-use TMB substrate. A polyclonal activator for human T cells (mAb CD3-2) is supplied as a positive control for cytokine secretion.. Pre-coated plates reduce assay time and minimize variability. In addition, strip-plates are ideal when running a limited number of wells.. Human IFN-γ ELISpotPLUS kits are also available with Streptavidin-ALP and BCIP/NBT-plus substrate. See Related products.. ...
This kit is ideal for users who want a convenient and sensitive assay. The assay is designed for the enumeration of cells secreting human IFN-γ. The kit includes ELISpot plates pre-coated with monoclonal antibody, biotinylated detection antibody, Streptavidin-ALP and ready-to-use BCIP/NBT-plus substrate. A polyclonal activator for human T cells (mAb CD3-2) is supplied as a positive control for cytokine secretion.. Pre-coated plates reduce assay time and minimize variability.. Human IFN-γ ELISpotPLUS kits are also available with Streptavidin-HRP and TMB substrate. See Related products.. ...
The IUPHAR/BPS Guide to Pharmacology. IFN-γ ligand page. Quantitative data and detailed annnotation of the targets of licensed and experimental drugs.
Long-term dyrket interferon-γ enzymbundet immunospot assay blir anvendt som et mål på sentralhukommelsesresponser og korrelerer med...
Clone REA600 recognizes the human interferon gamma (IFN-γ) antigen, a cytokine of type II class interferons. IFN-γ is produced by lymphocytes that are involved in inflammatory immune responses. It functions as a potent activator of macrophages, increases the antiviral and antitumor effects of the type I interferons, and has antiproliferative effects on transformed cells. IFN-γ is predominantly released by memory and effector CD4+ and CD8+ T cells as well as by NK cells upon activation. Additional information: Clone REA600 displays negligible binding to Fc receptors. - USA
Its important that the color of white be consistently neutral at all light levels from darkest to brightest. Grayscale performance impacts color accuracy with regard to the secondary colors: cyan, magenta, and yellow. While you can manipulate them wi...
Objectives. Interferon-β (IFN-β) is used in the treatment of multiple sclerosis (MS). IFN-β activation of signal transduction and activation of transcription (STAT)-4 is linked to its immunomodulatory effects. Previous studies suggest a type I IFN deficit in immune cells of MS patients, but data on interferon-α/β receptor (IFNAR) expression and the relationship with treatment response are conflicting. Here we compare IFN-β-mediated STAT4 activation in immune cells of untreated MS patients and controls ...
Gentaur molecular products has all kinds of products like :search , Panomics \ Procarta Cytokine Assay Kit, Mouse 10_plex \ PC0104 for more molecular products just contact us
Breaking News. A science team have released preliminary news that they can now very easily replicate proof of Cold Fusion / LENR with easy to get material.
Monoclonal Anti-Interferon-γ antibody produced in mouse (Anti-IFN-γ); monoclonal 25718, purified immunoglobulin, lyophilized powder; Suitable for neutralization; Interferons (IFNs) are cytokines that are secreted in response to viral or bacterial infections and tumorigenesis;
LAA049Hu82, FITC-Linked Monoclonal Antibody to Interferon Gamma (IFNg), 干扰素γ(IFNg)单克隆抗体(异硫氰酸荧光素标记), IFN-G; IFG; IFI; INFr; IFN, Immune Interferon | 仅供体外研究使用,不用于临床诊断!请索取进口关税税单及报关单!
APA049Hu61, Active Interferon Gamma (IFNg), 干扰素γ(IFNg)活性蛋白, IFN-G; IFG; IFI; INFr; IFN, Immune Interferon | 仅供体外研究使用,不用于临床诊断!请索取进口关税税单及报关单!
Identification of interferon-γ as the lymphokine that activates human macrophage oxidative metabolism and antimicrobial activity Academic Article ...
"The heparan sulfate binding sequence of interferon-gamma increased the on rate of the interferon-gamma-interferon-gamma ... 1fg9: 3:1 COMPLEX OF INTERFERON-GAMMA RECEPTOR WITH INTERFERON-GAMMA DIMER ... Interferon gamma (IFNγ) is a dimerized soluble cytokine that is the only member of the type II class of interferons.[5] The ... Interferon alpha (interferon alfa, IFN-α). *Interferon alfa (IFNA1, IFNA2, IFNA4, IFNA5, IFNA6, IFNA7, IFNA8, IFNA10, IFNA13, ...
... interferon gamma); or cause cell death (viz., tumor necrosis factor alpha). AGEP: Key elements promoting tissue injury in AGEP ...
In response to IL-12, MAIT cells produce and secrete interferon-gamma (IFN-γ), a cytokine that activate macrophages, assists in ... Boehm, U.; Klamp, T.; Groot, M.; Howard, J. C. (1997-01-01). "Cellular responses to interferon-gamma". Annual Review of ...
Interferon-gamma induces expression of AIM2. Though there has been virtually no biochemistry performed, a model based on cell- ... Interferon-inducible protein AIM2 also known as absent in melanoma 2 or simply AIM2 is a protein that in humans is encoded by ... Choubey D, Walter S, Geng Y, Xin H (2000). "Cytoplasmic localization of the interferon-inducible protein that is encoded by the ... "Cloning a novel member of the human interferon-inducible gene family associated with control of tumorigenicity in a model of ...
Gamma-interferon-inducible lysosomal thiol reductase is an enzyme that, in humans, is encoded by the IFI30 gene. The protein ... "Entrez Gene: IFI30 interferon, gamma-inducible protein 30". Csányi E (1977). "[Endre Kótai; 1914-1976]". Magyar traumatológia, ... The enzyme is expressed constitutively in antigen-presenting cells and induced by gamma-interferon in other cell types. This ... Phan UT, Arunachalam B, Cresswell P (2000). "Gamma-interferon-inducible lysosomal thiol reductase (GILT). Maturation, activity ...
IL-18 is known as a factor that induces the production of interferon gamma (IFN-γ). It is a pro-inflammatory cytokine that ... Billiau A (1996). "Interferon-gamma: biology and role in pathogenesis". Advances in Immunology. 62: 61-130. doi:10.1016/s0065- ... Dao T, Ohashi K, Kayano T, Kurimoto M, Okamura H (November 1996). "Interferon-gamma-inducing factor, a novel cytokine, enhances ... "Interleukin 18 together with interleukin 12 inhibits IgE production by induction of interferon-gamma production from activated ...
Tumor necrosis factor alpha and Interferon gamma). Induction of the high-output iNOS usually occurs in an oxidative environment ...
The cytokine interferon gamma and the Gram negative bacterial cell wall component lipopolysaccharide induce CD38 expression on ... Interferon gamma strongly induces CD38 expression on monocytes. The cytokine tumor necrosis factor strongly induces CD38 on ...
"E38N interferon gamma polypeptide variants - US Patent 7232562 Full Text". Archived from the original on 11 ... "Interferon gamma conjugates - US Patent 7230081 Full Text". Archived from the original on 11 October 2012. ... "Polynucleotides encoding interferon gamma polypeptides - US Patent 7504237 Full Text". Archived from the ...
Interleukin-18 (IL18, also known as interferon-gamma inducing factor) is a protein which in humans is encoded by the IL18 gene ... IL-18 is up regulated by interferon-gamma. IL-18 has also been found to increase the Alzheimer's disease-associated amyloid- ... June 1998). "Overview of interleukin-18: more than an interferon-gamma inducing factor". Journal of Leukocyte Biology. 63 (6): ... "Entrez Gene: IL18 interleukin 18 (interferon-gamma-inducing factor)". Yasuda K, Nakanishi K, Tsutsui H (February 2019). " ...
Singh, V. K. (1996). "Plasma increase of interleukin-12 and interferon-gamma. Pathological significance in autism". Journal of ...
Gamma-interferon-inducible protein Ifi-16 (Ifi-16) also known as interferon-inducible myeloid differentiation transcriptional ... "Entrez Gene: IFI16 interferon, gamma-inducible protein 16". Ouchi, Mutsuko; Ouchi, Toru (2008-01-01). "Role of IFI16 in DNA ... "A novel gene constitutively expressed in human lymphoid cells is inducible with interferon-gamma in myeloid cells". ... 2004). "The interferon-inducible IFI16 gene inhibits tube morphogenesis and proliferation of primary, but not HPV16 E6/E7- ...
Then, with IL-12 available, Th1 cells and NK cells produce interferon gamma (IFN-γ) molecules and subsequently release them. ... Cooper, A. M. (1 December 1993). "Disseminated tuberculosis in interferon gamma gene-disrupted mice". Journal of Experimental ...
"High Levels of Interferon Gamma in the Plasma of Children With Complete Interferon Gamma Receptor Deficiency". Pediatrics. 107 ... a complete deficiency in the interferon-gamma receptor have significant elevations in plasma concentrations of interferon-gamma ... "IFNGR1 interferon gamma receptor 1 [ Homo sapiens (human) ]". Bryant, Stephen H.; Kans, Jonathan A.; Chappey, Colombe; Geer, ... "Interferon-gamma-receptor deficiency in an infant with fatal bacille Calmette-Guérin infection". The New England Journal of ...
This test is based on gamma interferon blood test technology. The intention is that when necessary, it can be used alongside ...
Antibiotika + svampemiddel + Interferon gamma. *Antibiotika: Trimethoprim/sulfamethoxazole[30] og evt. andre typer *0-6 mdr.: ... Interferon gamma 3x ugtl.: Nedsætter hyppigheden af infektioner med 70 % og giver mildere infektioner end normalt. ...
Fontana V, Choren V, Vauthay L, Calvo JC, Calvo L, Cameo M (December 2004). "Exogenous interferon-gamma alters murine inner ... Sharma B, Iozzo RV (February 1998). "Transcriptional silencing of perlecan gene expression by interferon-gamma". J. Biol. Chem ... Interferon- γ treatment of blastocyst-stage murine embryos leads to a loss of perlecan expression on the trophectoderm, and ... Interferon-γ signaling mediates transcriptional repression of the perlecan gene. This was first shown in colon cancer cell ...
Haque MF, Meghji S, Nazir R, Harris M (January 2001). "Interferon gamma (IFN-gamma) may reverse oral submucous fibrosis". ... IFN-gamma is antifibrotic cytokine which alters collagen synthesis and helps in OSF. Colchicine tablets 0.5 mg twice a day ...
... like interferon gamma, that activate macrophages and cytotoxic T lymphocytes (CTLs). Interferon gamma represents an example of ... Once activated by T lymphocytes or Natural Killer cells, interferon gamma upregulates expression of macrophages and both types ... In B lymphocytes (B cells), interferon gamma stimulates antibody class switching. All of these cells have different, ...
Huang KJ, Su IJ, Theron M, Wu YC, Lai SK, Liu CC, Lei HY (February 2005). "An interferon-gamma-related cytokine storm in SARS ... The high mortality rate of COVID-19 in older populations were linked to the impact of age on interferon responses. Short-term ... An animal study done on mice found that those producing early strong interferon responses to SARS-CoV, which also may have ... Due to the increased levels of cytokines and interferons in those with severe Sars-CoV-2, both are being investigated as ...
IL-10 or Interferon gamma) in the tumor environment. The products of these two genes, programmed death-ligand 1 and programmed ...
Expression of this catalytic subunit (beta 2, according to systematic nomenclature) is downregulated by gamma interferon due to ... Rivett AJ, Bose S, Brooks P, Broadfoot KI (2001). "Regulation of proteasome complexes by gamma-interferon and phosphorylation ... "Newly identified pair of proteasomal subunits regulated reciprocally by interferon gamma". The Journal of Experimental Medicine ...
Dorman SE, Holland SM (2000). "Interferon-gamma and interleukin-12 pathway defects and human disease" (PDF). Cytokine & Growth ... Rosenzweig SD, Holland SM (February 2005). "Defects in the interferon-gamma and interleukin-12 pathways". Immunological Reviews ... due to decreased production of interferons in the central nervous system. Interferon-γ (IFN-γ)/interleukin-12 (IL-12) pathway ... UNC93B1 is very important signaling molecule involved in the production of interferon which plays a key role in the killing of ...
The vaccine resulted in specific interferon gamma cell production. Also specific T-cells for middle envelop proteins antigens ... and interferon gamma". Proceedings of the National Academy of Sciences of the United States of America. 93 (7): 2879-83. ... Yi AK, Chace JH, Cowdery JS, Krieg AM (January 1996). "IFN-gamma promotes IL-6 and IgM secretion in response to CpG motifs in ... Both helper and cytotoxic T-cells can control viral infections by secreting interferons. Cytotoxic T cells usually kill virally ...
Ashkar AA, Di Santo JP, Croy BA (July 2000). "Interferon gamma contributes to initiation of uterine vascular modification, ... Depletion of the inflammatory cytokine interferon gamma reversed the effect.[citation needed] Since NK cells recognize target ... NKT cell activity promotes NK cell activity by secreting interferon gamma. In contrast to NKT cells, NK cells do not express T- ... NK cells are activated in response to interferons or macrophage-derived cytokines. They serve to contain viral infections while ...
Induction by interferon-gamma and contribution to ATP-dependent proteolysis". The Journal of Biological Chemistry. 275 (19): ...
Induction by interferon-gamma and contribution to ATP-dependent proteolysis". J. Biol. Chem. 275 (19): 14336-45. doi:10.1074/ ...
Induction by interferon-gamma and contribution to ATP-dependent proteolysis". The Journal of Biological Chemistry. 275 (19): ...
This gene encodes the beta subunit of the 11S regulator, one of the two 11S subunits that is induced by gamma-interferon. Three ... Induction by interferon-gamma and contribution to ATP-dependent proteolysis". J. Biol. Chem. 275 (19): 14336-45. doi:10.1074/ ... a gamma-interferon-inducible protein activator of the 20S proteasome". FEBS Lett. 366 (1): 37-42. doi:10.1016/0014-5793(95) ... Wójcik C, Tanaka K, Paweletz N, Naab U, Wilk S (1999). "Proteasome activator (PA28) subunits, alpha, beta and gamma (Ki antigen ...
"Entrez Gene: ISGF3G interferon-stimulated transcription factor 3, gamma 48kDa". Horvath, C M; Stark G R; Kerr I M; Darnell J E ... "Subunit of an alpha-interferon-responsive transcription factor is related to interferon regulatory factor and Myb families of ... Interferon regulatory factor 9 is a protein that in humans is encoded by the IRF9 gene, previously known as ISGF3G. IRF9 has ... 2002). "Resistance to interferons in melanoma cells does not correlate with the expression or activation of signal transducer ...
... plasma cells will likely secrete IgG3 antibodies if they matured in the presence of the cytokine interferon-gamma. Since B cell ...
... ability to produce and respond to interferon proteins such as interferon-alpha, interferon-beta, and interferon gamma.[51][95] ... proteins including interferon regulatory factor 3 and interferon regulatory factor 7 trigger a signalling cascade that leads to ... the expression of type 1 interferons.[51] The type 1 interferons are then released and bind to the IFNAR1 and IFNAR2 receptors ... This triggers the expression of interferon-stimulated genes, which code for proteins with antiviral properties.[51] EBOV's V24 ...
Gamma-interferon transcriptionally regulates an early-response gene containing homology to platelet proteins". Nature. 315: 672 ... Neville LF, Mathiak G, Bagasra O (1998). „The immunobiology of interferon-gamma inducible protein 10 kD (IP-10): a novel, ... Angiolillo (1995). „Human interferon-inducible protein 10 is a potent inhibitor of angiogenesis in vivo". J. Exp. Med. 182: 155 ... Dufour (2002). „IFN-gamma-inducible protein 10 (IP-10; CXCL10) -deficient mice reveal a role for IP-10 in effector T cell ...
positive regulation of interferon-gamma production. • blastocyst formation. • positive regulation of interleukin-4 production. ...
"The transmembrane CXC-chemokine ligand 16 is induced by IFN-gamma and TNF-alpha and shed by the activity of the disintegrin- ...
Abnormal neutrophil chemotaxis due to decreased production of interferon gamma by T lymphocytes is thought to cause the disease ... Borges W, Augustine N, Hill H (2000). "Defective interleukin-12/interferon-gamma pathway in patients with ... High-dose intravenous gamma-globulin has also been suggested for the treatment of severe eczema in patients with HIES and ... Kimata H (1995). "High-dose intravenous gamma-globulin treatment for hyperimmunoglobulinemia E syndrome". J Allergy Clin ...
Interferon alfa natural. *Interferon alfa 2a / Peginterferon alfa-2a. *Interferon alfa 2b / Peginterferon alfa-2b / ...
The CDC recommends either a blood test, also known as an interferon-gamma release assay (IGRA), or a skin test, also known as a ...
Fukuoka M, Yasuda K, Fujiwara H, Kanzaki H, Mori T. Interactions between interferon gamma, tumour necrosis factor alpha, and ...
positive regulation of interferon-gamma production. • embryonic axis specification. • labyrinthine layer blood vessel ...
GAG - gamma globulin - gamma interferon - ganglion - GART - gastrointestinal (GI) - gene - gene therapy - genetic engineering ... alpha interferon (INFa) - alternative medicine - alveolar - amebiasis - amino acids - anaphylactic shock - anemia - anergy - ... interferon - interleukin-1 (IL-1) - interleukin-2 (IL-2) - interleukin-4 (IL-4) - interleukin-12 (IL-12) - interleukins - ...
positive regulation of interferon-gamma production. • positive regulation of interleukin-17 production. • innate immune ...
... interferon gamma. The proteasome assembled with these alternative subunits is known as the immunoproteasome, whose substrate ... whose expression is induced by interferon gamma, are the primary producers of peptides which are optimal in size and ... The expression of the 11S particle is induced by interferon gamma and is responsible, in conjunction with the immunoproteasome ...
Type I interferons (IFN), secreted mainly by dendritic cells,[22] play a central role in antiviral host defense and a cell's ... Main article: gamma/delta T cells. Like other 'unconventional' T cell subsets bearing invariant T cell receptors (TCRs), such ... Le Bon A, Tough DF (August 2002). "Links between innate and adaptive immunity via type I interferon". Current Opinion in ... "Influenza A virus lacking the NS1 gene replicates in interferon-deficient systems". Virology. 252 (2): 324-30. doi:10.1006/viro ...
Gaviria JM, van Burik JA, Dale DC, Root RK, Liles WC (April 1999). "Comparison of interferon-gamma, granulocyte colony- ...
Interferon alpha (interferon alfa, IFN-α). *Interferon alfa (IFNA1, IFNA2, IFNA4, IFNA5, IFNA6, IFNA7, IFNA8, IFNA10, IFNA13, ... Agonists: Interferon gamma (IFN-γ). *Interferon gamma 1b. *Antibodies: Emapalumab. *Fontolizumab. IFNLR (λ, III). *See IL-28R ( ...
Chen, J; Zhang, R, Wang, J, Liu, L, Zheng, Y, Shen, Y, Qi, T, Lu, H (2011). "Interferon-gamma release assays for the diagnosis ... preporučuje se učiniti interferon gamma release assays (IGRAs) u uzorku krvi.[59] Imunizacija na većinu mikobakterija ne utječe ... Metcalfe, JZ; Everett, CK, Steingart, KR, Cattamanchi, A, Huang, L, Hopewell, PC, Pai, M (2011-11-15). "Interferon-γ release ... U zemljama u razvoju se ne izvode interferon-γ release assays (IGRAs) niti tuberkulinski kožni test.[54][55] U osoba zaraženih ...
positive regulation of interferon-gamma production. • response to glucocorticoid. • positive regulation of vitamin D ...
cellular response to interferon-gamma. Sources:Amigo / QuickGO. Orthologs. Species. Human. Mouse. ... Fc-gamma receptor signaling pathway involved in phagocytosis. • protein complex assembly. • actin filament organization. • ...
"Serum Collagen Type IV for the Assessment of Fibrosis and Resistance to Interferon Therapy in Chronic Hepatitis C". ...
negative regulation of interferon-gamma production. • response to cadmium ion. • negative regulation of calcineurin-NFAT ...
Upregulation of interferon gamma, IL-2 and IL-10 have been reported for pomalidomide and may contribute to its anti-angiogenic ...
For example, therapeutic efficacy of recombinant human interferon gamma, expressed in HEK 293 platform, was improved against ... "Improved therapeutic efficacy of mammalian expressed-recombinant interferon gamma against ovarian cancer cells". Experimental ...
CD4+ T cells secrete IL-2 and interferon gamma, inducing the further release of other Th1 cytokines, thus mediating the immune ... After several weeks, the immune system somehow [mechanism as yet unexplained] ramps up and, on stimulation with IFN-gamma, the ...
... monokine induced by gamma interferon (MIG), and interferon gamma-induced protein 10 (IP-10). Cytokines may also contribute to ... Viral replication is highly cytopathic, but susceptible to type-I and -II interferon.[43] In vivo, in studies using living ... Upon infection with chikungunya, the host's fibroblasts produce type-1 alpha and beta interferon (IFN-α and IFN-β).[44] In ... The type-1 interferon response seems to play an important role in the host's response to chikungunya infection. ...
Interferon-gamma. References[edit]. *^ Julius M. Cruse; Robert Edwin Lewis (20 April 2009). Illustrated dictionary of ... Interferon alpha (interferon alfa, IFN-α). *Interferon alfa (IFNA1, IFNA2, IFNA4, IFNA5, IFNA6, IFNA7, IFNA8, IFNA10, IFNA13, ...
Lalvani, A., Richeldi, L., Kunst, H. Interferon gamma assays for tuberculosis. The Lancet Infectious Diseases. 2005, roč. 5, ... rentgenu), tuberkulinového testu, histopatologie[5] anebo pomocí Interferon Gamma Release Assay (IGRA), ale tyto metody jsou ... Interferon Gamma Release Assay - IGRA) některé z těchto problémů překonaly. Testy IGRA jsou krevní testy in vitro a jsou ...
... and interferon gamma (IFN-. .mw-parser-output .polytonic{font-family:"SBL BibLit","SBL Greek",Athena,"EB Garamond 12","Foulis ...
... interferon gamma (IFN-γ) or after infection of monocytes herpes virus, Mycobacterium tuberculosis and Candida albicans (Figure ... and the common gamma chain (gamma-C, CD132). IL-15 is secreted by mononuclear phagocytes (and some other cells) following ...
"The heparan sulfate binding sequence of interferon-gamma increased the on rate of the interferon-gamma-interferon-gamma ... 1fg9: 3:1 COMPLEX OF INTERFERON-GAMMA RECEPTOR WITH INTERFERON-GAMMA DIMER ... Interferon gamma (IFNγ) is a dimerized soluble cytokine that is the only member of the type II class of interferons.[5] The ... Interferon alpha (interferon alfa, IFN-α). *Interferon alfa (IFNA1, IFNA2, IFNA4, IFNA5, IFNA6, IFNA7, IFNA8, IFNA10, IFNA13, ...
Interferons: beta interferon by fibroblasts, and gamma interferon by natural killer cells and cytotoxic T lymphocytes (killer T ... Interferons exert additional inhibitory effects by regulating the extent to which lymphocytes and other cells express certain ... cells). All interferons inhibit viral replication by interfering with the transcription of viral nucleic acid. ... Other articles where Gamma interferon is discussed: immune system: ...
Shawn P. Murphy, Chandrakant Tayade, Ali A. Ashkar, Kota Hatta, Jianhong Zhang, and B. Anne Croy "Interferon Gamma in ... Interferon Gamma in Successful Pregnancies. Shawn P. Murphy, Chandrakant Tayade, Ali A. Ashkar, Kota Hatta, Jianhong Zhang, B. ... Interferon gamma has broader roles in activation of innate and adaptive immune responses to viruses and tumors, in part through ... Interferon gamma (IFNG) is a proinflammatory cytokine secreted in the uterus during early pregnancy. It is abundantly produced ...
interferon gamma receptor 1. Names. AVP, type 2. CD119 antigen. CDw119. IFN-gamma receptor 1. IFN-gamma-R-alpha. IFN-gamma-R1. ... IFNGR1 interferon gamma receptor 1 [Homo sapiens] IFNGR1 interferon gamma receptor 1 [Homo sapiens]. Gene ID:3459 ... This gene (IFNGR1) encodes the ligand-binding chain (alpha) of the gamma interferon receptor. Human interferon-gamma receptor ... The Distribution Frequency of Interferon-Gamma Receptor 1 Gene Polymorphisms in Interferon-γ Release Assay-Positive Patients. ...
Gamma interferon may have potential to prevent Ebola infection The recent Ebola outbreak in West Africa has claimed more than ... nor interferon-gamma release assays (IGRAs) are capable of distinguishing active from latent infection or predicting the chance ... and found that SeV C protein inhibits the signal transduction pathway of interferon gamma. ...
"The heparan sulfate binding sequence of interferon-gamma increased the on rate of the interferon-gamma-interferon-gamma ... Interferon-γ has been shown to interact with Interferon gamma receptor 1. Interferon-γ has been shown to be a crucial player in ... Interferon gamma (IFNγ) is a dimerized soluble cytokine that is the only member of the type II class of interferons. The ... Schneider LC, Baz Z, Zarcone C, Zurakowski D (March 1998). "Long-term therapy with recombinant interferon-gamma (rIFN-gamma) ...
ACTIMMUNE (interferon gamma-1b) injection, solution NDC Code(s): 75987-111-10, 75987-111-11 ... ACTIMMUNE (interferon gamma-1b) injection, solution NDC Code(s): 42238-111-01, 42238-111-12 ...
Defective in vitro gamma-interferon production in rheumatoid arthritis.. Malaise MG, Franchimont P. ...
It binds interferon-γ, the sole member of interferon type II. The human interferon-gamma receptor complex consists the ... Pestka S, Kotenko SV, Muthukumaran G, Izotova LS, Cook JR, Garotta G (1997). "The interferon gamma (IFN-gamma) receptor: a ... Interferon-γ Receptor-1 and 2 Mutations Interferon gamma receptor (IFNGR1) family. ... Interferon-gamma-receptor deficiency in an infant with fatal bacille Calmette-Guerin infection. N Engl J Med. 1996;335:1956- ...
Find the most comprehensive real-world treatment information on Interferon gamma-1a at PatientsLikeMe. 0 patients with ... bipolar I disorder or psoriasis currently take Interferon gamma-1a. ...
The flu-like side effects of interferon may be stressful to heart patients. ...
Interferons help the body fight viral infections. Interferon gamma-1b is used to prevent serious infections in people with a ... Interferon gamma-1b is also used to slow the progression of a bone disorder called malignant... ... Interferon gamma-1b is made from human proteins. ... What is interferon gamma-1b?. Interferon gamma-1b is made from ... What is the most important information I should know about interferon gamma-1b?. Interferon gamma-1b can lower blood cells that ...
Buy our Recombinant Sheep Interferon gamma protein. Ab169711 is a full length protein produced in Yeast and has been validated ... Recombinant Sheep Interferon gamma protein. See all Interferon gamma proteins and peptides. ... IFN-gamma, in addition to having antiviral activity, has important immunoregulatory functions. It is a potent activator of ... including IFN-gamma, which would in turn be able to suppress hematopoiesis. ...
Rabbit polyclonal Interferon gamma antibody. Validated in WB and tested in Chicken. Immunogen corresponding to recombinant full ... Primary - Rabbit Anti-Interferon gamma antibody (ab136390) WB Protein - Recombinant Human Interferon gamma protein (Active) ( ... IFN-gamma, in addition to having antiviral activity, has important immunoregulatory functions. It is a potent activator of ... including IFN-gamma, which would in turn be able to suppress hematopoiesis. ...
... Iukary Takenami,1 Camila Loureiro,2 ... T. Kasama, R. M. Strieter, N. W. Lukacs, P. M. Lincoln, M. D. Burdick, and S. L. Kunkel, "Interferon gamma modulates the ... Recently, a new sensitive and more specific test that has been developed to diagnose the infection is known as interferon-gamma ... A. Machado, K. Emodi, I. Takenami et al., "Analysis of discordance between the tuberculin skin test and the interferon-gamma ...
Learn about the potential side effects of interferon gamma-1b. Includes common and rare side effects information for consumers ... Check with your doctor or nurse immediately if any of the following side effects occur while taking interferon gamma-1b:. More ... Applies to interferon gamma-1b: subcutaneous solution. Gastrointestinal. Very common (10% or more): Diarrhea, nausea, vomiting ... Applies to interferon gamma-1b: solution. Side effects requiring immediate medical attention. Along with its needed effects, ...
Interferon Gamma 1 b) may treat, uses, dosage, side effects, drug interactions, warnings, patient labeling, reviews, and ... interferon gamma-1b) Subcutaneous Injection. DESCRIPTION. ACTIMMUNE (Interferon gamma-1b), an interferon gamma, is a single- ... Type 1 interferons (alpha and beta) bind to the alpha/ beta receptor. Interferon gamma binds to a different cell surface ... Female mice receiving recombinant murine IFN-interferon gamma (rmuIFN-gamma) at 32 times the maximum recommended clinical dose ...
Adenosine deaminase and interferon gamma measurements for the diagnosis of tuberculous pleurisy: a meta-analysis ... and interferon-gamma (IFN-γ). Here we summarise the literature on ADA and IFN-γ as predictors of tuberculous pleurisy.. METHODS ... Keywords: adenosine deaminase; interferon type II; laboratory techniques; meta-analysis; procedure; tuberculous pleurisy ...
There are no specific protocols for Recombinant Human Interferon gamma protein (ab53375). Please download our general protocols ...
Interferon Gamma-1a (UNII: VC9M78242P) (Interferon Gamma-1a - UNII:VC9M78242P) Interferon Gamma-1a. 200 [kp_C] in 1 mL. ... G-12- interleukin-12 human, interferon gamma-1a spray. To receive this label RSS feed. Copy the URL below and paste it into ... G-12- interleukin-12 human, interferon gamma-1a spray. Out of scope - Out of scope for RxNorm and will not receive RxNorm ... G-12- interleukin-12 human, interferon gamma-1a spray. If this SPL contains inactivated NDCs listed by the FDA initiated ...
Interferon-γ signaling from meningeal T cells to neural circuits may represent a molecular co-evolutionary link between anti- ... T cells are "we" cells: Interferon-gamma affects social behavior. By Catherine Hagan, D.V.M., Ph.D. No mouse is an island. ... Unexpected role of interferon-γ in regulating neuronal connectivity and social behaviour. Nature. Jul 21;535(7612):425-9. PMID ... Jonathan Kipnis at the University of Virginia demonstrates a critical role for meningeal interferon-γ (IFN-γ) in mediating both ...
Drug: Interferon gamma-1b Participant 1: 2 drops of topical interferon gamma-1b three times daily in the study eye for a daily ... The drug interferon gamma-1b can help reduce fluid accumulation in the retina. Researchers want to see if interferon gamma-1b ... Experimental: Interferon gamma-1b Interferon gamma-1b (Actimmune®, InterMune, Inc, Brisbane, CA 94005) was supplied to ... All participants will receive interferon gamma-1b for two weeks. Doses of interferon gamma-1b eye drops will be escalated among ...
1. gamma-interferon (n.). a form of interferon that is produced by T cells and macrophages; involved in the activation of ... 3. beta-interferon (n.). a form of interferon that is produced by fibroblasts and have antiviral effects; used in the treatment ... 2. alpha-interferon (n.). a form of interferon that is produced endogenously and commercially for its pharmacological effects ( ... 7. Gamma (n.). Portuguese navigator who led an expedition around the Cape of Good Hope in 1497; he sighted and named Natal on ...
Interferon-gamma, the Th1/Th2 paradigm and autoimmune diseases]. Download Prime PubMed App to iPhone, iPad, or Android ... in which interferon-gamma (IFN-gamma), produced by NK and T lymphocytes, occupies a central position. IFN-gamma influences the ... in which interferon-gamma (IFN-gamma), produced by NK and T lymphocytes, occupies a central position. IFN-gamma influences the ... How interferon-gamma keeps autoimmune diseases in check.. *Endogenous IL-4 and IFN-gamma are essential for expression of Th2, ...
We investigated the effects of two cytokines from T lymphocytes, interleukin-4 (IL-4) and interferon-gamma (IFN-gamma) … ... We investigated the effects of two cytokines from T lymphocytes, interleukin-4 (IL-4) and interferon-gamma (IFN-gamma), on ... Antagonistic effects of interferon-gamma and interleukin-4 on fibroblast cultures J Invest Dermatol. 1997 Aug;109(2):158-62. ... This effect might explain the antagonistic effects of IFN-gamma on the IL-4-dependent enhancement of collagen synthesis. Thus, ...
Interferon-gamma: the major mediator of resistance against Toxoplasma gondii. By Y Suzuki, MA Orellana, RD Schreiber, JS ... Interferon-gamma: the major mediator of resistance against Toxoplasma gondii. By Y Suzuki, MA Orellana, RD Schreiber, JS ... Interferon-gamma: the major mediator of resistance against Toxoplasma gondii Message Subject. (Your Name) has forwarded a page ... The fact that an antibody to interferon-gamma can eliminate resistance to acute Toxoplasma infection in mice suggests that this ...
Gamma interferon (IFNγ), a type II IFN, was discovered approximately seven years after discovery of type I IFNs. IFNs are ... Gamma interferon (IFNγ), a type II IFN, was discovered approximately seven years after discovery of type I IFNs. IFNs are ... Gamma interferon (IFNγ), a type II IFN, was discovered approximately seven years after discovery of type I IFNs. IFNs are ...
The interferon-gamma receptor (IFNGR) is a receptor that binds interferon-γ, the sole member of interferon type II. ... Interferon alpha (interferon alfa, IFN-α). *Interferon alfa (IFNA1, IFNA2, IFNA4, IFNA5, IFNA6, IFNA7, IFNA8, IFNA10, IFNA13, ... Pestka S, Kotenko SV, Muthukumaran G, Izotova LS, Cook JR, Garotta G (1997). "The interferon gamma (IFN-gamma) receptor: a ... The human interferon-gamma receptor complex consists the heterodimer of two chains: IFNGR1 and IFNGR2.[2][3] In unstimulated ...
Production of gamma interferon in mice immune to Rickettsia tsutsugamushi. Infect Immun. 43: 59.Google Scholar ... Jerrells T.R., Li H., Walker D.H. (1988) In Vivo and in Vitro Role of Gamma Interferon in Immune Clearance of Rickettsia ... Gamma interferon production in response to homologous and heterologous strain antigens in mice chronically infected with ... In Vivo and in Vitro Role of Gamma Interferon in Immune Clearance of Rickettsia species. ...
  • Interferon gamma ( IFNγ ) is a dimerized soluble cytokine that is the only member of the type II class of interferons . (
  • IFNγ, or type II interferon, is a cytokine that is critical for innate and adaptive immunity against viral, some bacterial and protozoal infections. (
  • Interferon gamma (IFNG) is a proinflammatory cytokine secreted in the uterus during early pregnancy. (
  • J Interferon Cytokine Res. (
  • However, the formation of autoantibodies and autoreactive inflammatory cells is also regulated by the cytokine network, in which interferon-gamma (IFN-gamma), produced by NK and T lymphocytes, occupies a central position. (
  • As a cytokine, interferon-gamma is a highly versatile homodimeric protein that plays an essential role in cell mediated immune responses to viral and mycobacterial infections. (
  • Biochemical characterization of a gamma interferon-inducible cytokine (IP-10). (
  • Interferon-gamma (IFNg) is a central cytokine in modulating innate and adaptive immunity in a wide range of vertebrates. (
  • However, as IFN-gamma is produced by both fully rested and more recently activated populations of memory T cells, it is not surprising that the measurement of this cytokine alone cannot accurately distinguish Latent TB Infected (LTBI) subjects from those with active (infectious) disease. (
  • Interferon gamma (IFN-γ) is a T helper cell type 1 (Th1) cytokine that plays important roles in almost all phases of immune and inflammatory responses. (
  • In 1988, the laboratory of Dr. Jeffrey Ravetch characterized a gene which is upregulated upon exposure to the cytokine, interferon-γ (IFN-γ). (
  • Among the potent antifibrotics, interferon gamma (IFN gamma), a proinflammatory cytokine, is highly efficacious but it failed in clinical trials due to the poor efficacy and multiple adverse effects attributed to the ubiquitous IFN gamma receptor (IFN gamma R) expression. (
  • Interferon-gamma (IFN-gamma), also known as Type II interferon or immune interferon, is a cytokine produced primarily by lymphocytes and natural killer cells. (
  • The cytokine gamma interferon (IFN-γ) contributes to the eradication of A. phagocytophilum ( 1 , 21 , 24 , 30 , 31 , 38 ). (
  • The zebrafish genome contains ten genes that encode class II cytokine-like peptides, of which the two that are related most closely to mammalian interferon gamma (IFN-γ) were named IFN-γ1 and IFN-γ2. (
  • The International Society for Interferon and Cytokine Research (ISICR), based in Bethesda, Md., is a non-profit organization devoted to interferon, cytokine and chemokine research in the fields of cell biology, molecular biology, biochemistry, and pre-clinical and clinical drug development. (
  • Neither the tuberculin skin test (TST) nor interferon-gamma release assays (IGRAs) are capable of distinguishing active from latent infection or predicting the chance of reactivation. (
  • Tuberculin skin test (TST) and interferon-gamma release assays (IGRAs) are the available tests to detect the infection. (
  • Recently, a new sensitive and more specific test that has been developed to diagnose the infection is known as interferon-gamma release assays (IGRAs). (
  • Fluid transport assays have examined whether interferon gamma induces changes in fluid transport across human fetal RPE monolayers and showed an increase in fluid absorption from the retinal to the choroidal side of the tissue. (
  • Interferon (IFN)-gamma release assays (IGRAs) have improved the accuracy of detection of MTB exposure in some subject groups as compared to the Tuberculin Skin Test (TST). (
  • Point-Counterpoint: Should Interferon Gamma Release Assays Become the Standard Method for Screening Patients for Mycobacterium tuberculosis Infections in the United States? (
  • During the past decade, however, in vitro CMI assays have been developed that use sensitive enzyme-linked immunosorbent assay (ELISA) or fluorescent measurements of T cell products, such as gamma interferon or ATP. (
  • These procedures have been given the common name of interferon gamma release assays (IGRAs). (
  • ELISA and ELISPOT methods are utilized for interferon-gamma (IFN-γ) release assays (IGRAs) to detect the IFN-γ secreted by T lymphocytes. (
  • IJERPH, Vol. 17, Pages 581: Risk of Occupational Latent Tuberculosis Infection among Health Personnel Measured by Interferon-Gamma Release Assays in Low Incidence Countries-A Systematic Review and Meta-Analysis International Journal of Environmental Research and Public Health doi: 10.3390/ijerph17020581 Authors: Claudia Peters Agnessa Kozak Albert Nienhaus Anja Schablon Healthcare workers (HCWs) have increased risk for latent tuberculosis infection (LTBI) and tuberculosis (TB) disease due to their occupational exposure. (
  • For some years now, interferon-γ release assays (IGRAs) have replaced the tuberculin skin test for the diagnosis of LTBI in many countries. (
  • We compared the usefulness of tuberculin skin test (TST) and three interferon-gamma release assays (IGRAs) (QuantiFERON-TB Gold (QFT-2G), QuantiFERON-TB Gold In-tube (QFT-3G), T-SPOT.TB) as the supportive method of diagnosing pulmonary tuberculosis (TB). (
  • We assessed the efficacy of serial interferon-gamma release assays (IGRAs) for the diagnosis and monitoring of latent tuberculosis infection (LTBI) in patients receiving immunosuppressive agents for treatment of rheumatic diseases, in an endemic area. (
  • Whenever epithelioid cell containing granulomas were present in paucibacillary forms, significant reactivity within the central phagocytic cells with the monoclonal antibody directed against interferon-gamma was detectable. (
  • Mice were injected with a monoclonal antibody to interferon-gamma to examine the importance of endogenous production of this lymphokine in resistance against infection with the sporozoan parasite Toxoplasma gondii. (
  • 1986. Neutralization of lymphokine-mediated antirickettsial activity of fibroblasts and macrophages with monoclonal antibody specific for murine interferon gamma. (
  • 1984. Monoclonal antibody to murine gamma interferon inhibits lymphokine-induced antiviral and macrophage tumoricidal activities. (
  • The induction of IFN gamma mRNA and protein by IL-2 was completely blocked by a monoclonal antibody to IL-2 receptor p75 (beta chain), but not by the monoclonal antibody to p55 (alpha chain). (
  • Interferon gamma (IFNG), a type II or immune interferon, is produced primarily by T lymphocytes and natural killer cells stimulated by alloantigens, tumors, and mitogens. (
  • and γ-interferons (also called immune interferons or interleukins), produced by immune reactions in blood lymphocytes. (
  • Interferon gamma-1b injection is used to reduce the frequency and severity of serious infections in people with chronic granulomatous disease (an inherited immune system disease). (
  • Interferon gamma has broader roles in activation of innate and adaptive immune responses to viruses and tumors, in part through upregulating transcription of genes involved in cell cycle regulation, apoptosis, and antigen processing/presentation. (
  • The existence of this interferon, which early in its history was known as immune interferon, was described by E. F. Wheelock as a product of human leukocytes stimulated with phytohemagglutinin, and by others as a product of antigen-stimulated lymphocytes. (
  • IFN-gamma influences the function of all cell types involved in immune-mediated inflammatory reactions: antigen-presenting cells, cytotoxic and regulatory T lymphocytes, antibody-producing B lymphocytes, endothelial cells and mononuclear phagocytes. (
  • 1982. Regulation of murine macrophage la antigen expression by a lymphokine with immune interferon activity. (
  • The primary aim of this study is to assess the effects of adjunctive therapy with Interferon (IFN)-gamma on immune function in patients with septic shock in a placebo-controlled manner. (
  • INTERFERON (in ter FEER on) Gamma-1b helps your immune system work better. (
  • Interferon-gamma (IFN-gamma) belongs to the type II interferon family and is secreted by activated immune cells-primarily T and NK cells, but also B-cells and APC. (
  • This study will evaluate the safety and effectiveness of the drug, interferon gamma, in treating leukocyte adhesion deficiency type I (LAD I). Patients with this inherited immune disorder do not have enough proteins called adhesion molecules on their infection-fighting white blood cells, impairing the ability of these cells to get to the site of infection. (
  • Neutralization of endogenous gamma interferon did not interfere with the induction of the cellular and humoral immune response but acted during the effector phase. (
  • Therefore, the actual study aimed to evaluate the location and quantitative distribution patterns of signature cytokines of type 1 [interferon gamma (IFN-γ)] or type 2 [interleukin (IL)-13] immune responses in vaccinated or infected hosts. (
  • To obtain information about the effects of interferon-gamma and aldesleukin on the number, function, and activation state of immune effector-cells including T-cells and B-cells. (
  • Although the zebrafish has become a popular model system to study immune mechanisms, and although interferons are central regulators of immunity, which zebrafish cytokines correspond functionally to mammalian IFN-γ has not been established. (
  • Gamma interferon, an immune lymphokine that protects mouse macrophages against infection by several parasites, was ineffective against Mycobacterium lepraemurium. (
  • To date their published studies have shown that laughing lowers blood pressure, reduces stress hormones, increases muscle flexion, and boosts immune function by raising levels of infection-fighting T-cells, disease-fighting proteins called Gamma-interferon and B-cells, which produce disease-destroying antibodies. (
  • An increase in gamma interferon, which tells various components of the immune system to 'turn on. (
  • The fact that an antibody to interferon-gamma can eliminate resistance to acute Toxoplasma infection in mice suggests that this lymphokine is an important mediator of host resistance to this parasite. (
  • Interferon gamma Antibody from HUABIO INC. (
  • Cellular responses to IFNγ are activated through its interaction with a heterodimeric receptor consisting of Interferon gamma receptor 1 (IFNGR1) and Interferon gamma receptor 2 (IFNGR2). (
  • All were discovered to have the same phenotype with the same mutation, which was located in the interferon-gamma (IFNGR) receptor, so the group began to explore what was causing this dysfunction. (
  • This gene (IFNGR1) encodes the ligand-binding chain (alpha) of the gamma interferon receptor. (
  • Human interferon-gamma receptor is a heterodimer of IFNGR1 and IFNGR2. (
  • Influence of interferon-gamma Receptor 1 gene polymorphisms on the susceptibility to pulmonary tuberculosis among sudanese population. (
  • Whole-exome sequencing reveals a rare interferon gamma receptor 1 mutation associated with myasthenia gravis. (
  • The Distribution Frequency of Interferon-Gamma Receptor 1 Gene Polymorphisms in Interferon- γ Release Assay-Positive Patients. (
  • Association of Interferon- γ Receptor-1 Gene Polymorphism with Nontuberculous Mycobacterial Lung Infection among Iranian Patients with Pulmonary Disease. (
  • The interferon-gamma receptor (IFNGR) protein complex is the heterodimer of two chains: IFNGR1 and IFNGR2. (
  • The human interferon-gamma receptor complex consists the heterodimer of two chains: IFNGR1 and IFNGR2. (
  • Interferon-gamma-receptor deficiency in an infant with fatal bacille Calmette-Guerin infection. (
  • The interferon-gamma receptor ( IFNGR ) is a receptor that binds interferon-γ , the sole member of interferon type II . (
  • INFG exerts its effect on cells by interacting with the specific IFN-gamma receptor (IFNGR). (
  • Stoichiometry of interaction between interferon gamma and its receptor. (
  • Interferon-gamma induces tyrosine phosphorylation of interferon-gamma receptor and regulated association of protein tyrosine kinases, Jak1 and Jak2, with its receptor. (
  • Preassembly and ligand-induced restructuring of the chains of the IFN-gamma receptor complex: the roles of Jak kinases, Stat1 and the receptor chains. (
  • Seeing the light: preassembly and ligand-induced changes of the interferon gamma receptor complex in cells. (
  • Interferon gamma-induced transcription of the high-affinity Fc receptor for IgG requires assembly of a complex that includes the 91-kDa subunit of transcription factor ISGF3. (
  • With regard to the mechanism by which selection was conferred, we found normal levels of IFN-gamma receptor mRNA, but a 60% reduction in expression of the IL-1 receptor type I (IL-1RI) in INS-1res cells compared with parental INS-1 cells. (
  • A cDNA encoding the human interferon-gamma receptor was isolated from a lambda gt11 expression library using a polyclonal antireceptor antiserum. (
  • The human interferon-gamma receptor expressed in mouse cells displayed the same binding properties as in human cells. (
  • However, transfected cells were not sensitive to human IFN-gamma, suggesting the need for species-specific cofactors in receptor function. (
  • As inferred from the cDNA sequence, the human interferon-gamma receptor shows no similarities to known proteins and represents a novel transmembrane receptor. (
  • IFN-gamma induces the production of cytokines, upregulates the expression of class I and II MHC antigens, Fc receptor and leukocyte adhesion molecules. (
  • Southern blot analysis indicates the presence of an Ig heavy chain gene rearrangement, but no rearrangement of the T-cell receptor beta chain gene or IFN gamma gene in these B-cell lines. (
  • After activation of the cell lines with IL-2, (-)Indolactam-v, and PMA, increases in cytoplasmic messenger RNAs (mRNAs) of IFN gamma and the IL- 2 receptor chains were also observed. (
  • This effect of IL-12 can be diminished by anti-interferon (IFN)-gamma mAb. (
  • These infections may also occur in previously immunocompetent patients with acquired anti-interferon-gamma antibodies (anti-IFN-γ Abs). (
  • The researchers examined the crystal structure of the complex of SeV C protein and transcription factor STAT1, and found that SeV C protein inhibits the signal transduction pathway of interferon gamma. (
  • These studies demonstrate the ability of interferon-gamma (IFN-gamma) to significantly enhance IL-6 mRNA and protein production in LPS-stimulated monocytes and THP-1 cells. (
  • IL-6 protein production was increased sevenfold in LPS-stimulated cells with the addition of IFN-gamma. (
  • A cell line lacking Stat1 (U3A) was not growth-arrested by IFN-alpha or IFN-gamma, and experiments were carried out with U3A cells permanently expressing normal or various mutant forms of Stat1 protein. (
  • An IFN-gamma-inducible protein, IP-10, has previously been described to belong to a gene family of chemotactic and mitogenic proteins, associated with inflammation and proliferation. (
  • These reagents establish that the IP-10 protein is secreted from a variety of cells (endothelial, monocyte, fibroblast, and keratinocyte) in response to IFN-gamma. (
  • Activation of transcription by IFN-gamma: tyrosine phosphorylation of a 91-kD DNA binding protein. (
  • Induction of guanylate binding protein 5 by gamma interferon increases susceptibility to Salmonella enterica serovar Typhimurium-induced pyroptosis in RAW 264.7 cells. (
  • FDA Researchers developed a combination treatment of a recombinant chimeric protein comprising of an anti-interleukin-4 (IL-4) conjugated to a Pseudomonas exotoxin (IL-4-PE38KDEL), interferon alpha (IFNα) and interferon beta (IFNγ). (
  • So has the inhibition of γ-interferon synthesis by EBV-encoded Bam HI C fragment rightward reading-frame no. 1 (BCRFI) protein [5] . (
  • Human interferon-gamma (IFN-gamma) is an important immunomodulatory protein produced predominantly by T cells and large granular lymphocytes (LGLs). (
  • We now report that PA682BM-1 can be triggered by the protein kinase C (PKC) activators, phorbol 12-myristate 13-acetate (PMA) and (-)Indolactam-v, to secrete IFN gamma, whereas JLP(c) cells spontaneously produce low levels of IFN gamma that can be enhanced by PKC activators and interleukin-2 (IL-2). (
  • PRAME is a golgi-targeted protein that associates with the Elongin BC complex and is upregulated by interferon-gamma and bacterial PAMPs. (
  • ACTIMMUNE (Interferon gamma-1b), an interferon gamma, is a single-chain polypeptide containing 140 amino acids . (
  • Each 0.5 mL of ACTIMMUNE contains: 100 mcg (2 million International Units) of interferon gamma-1b formulated in disodium succinate hexahydrate (0.37 mg), mannitol (20 mg), polysorbate 20 (0.05 mg), succinic acid (0.14 mg) and Sterile Water for Injection. (
  • ACTIMMUNE (interferon gamma-1b) is a sterile, clear, colorless solution filled in a single-use vial for subcutaneous injection. (
  • Each 0.5 mL of ACTIMMUNE contains: 100 mcg (2 million International Units) of interferon gamma-1b. (
  • We market Actimmune® (interferon gamma-1b), approved for the treatment of severe, malignant osteopetrosis and chronic granulomatous disease. (
  • These results suggest that immunologically induced interferon may be involved in controlling malaria infection under natural conditions. (
  • Interferon gamma release assay (IGRA) results were used as reference to assess performance of TST induration thresholds for detection of M. tuberculosis infection in children. (
  • We show here that IFN-gamma can be produced by scid mice in the absence of lymphocyte-mediated immunity, and this IFN-gamma is important for macrophage activation during infection with Listeria. (
  • These results indicate the presence of an important T lymphocyte-independent mechanism of macrophage activation and IFN-gamma production in response to infection. (
  • We conclude that γ-interferon may be useful in treating some patients with chronic active EBV infection. (
  • The results of the experimental study on the effect of the natural and recombinant gamma-interferons (gamma-IFs) of mice on the process of the infection caused by Klebsiella sp. (
  • Simultaneous treatment of macrophages with gamma interferon and interleukin-4 or interleukin-2 or a combination of all three did not enhance the macrophage resistance to infection with M. lepraemurium, but instead stimulated growth of M. lepraemurium. (
  • Infection of BALB/c mice with PR8 induced a multifunctional CD4 population with proliferative capacity and ability to secrete interleukin-2 (IL-2) and tumor necrosis factor alpha (TNF-α) in the draining lymph node (DLN) and gamma interferon (IFN-γ) and IL-10 in the lung. (
  • Interferons are now known to be a subset of a large group of natural cellular substances called cytokines, which signal cells to perform specific functions. (
  • T-lymphocytes, activated by an endogenous or exogenous, and most often unknown antigenic stimulus, secrete cytokines, including IFN-gamma, which would in turn be able to suppress hematopoiesis. (
  • Interferons (IFNs) are cytokines that are secreted in response to viral or bacterial infections and tumorigenesis. (
  • Pro-inflammatory cytokines like interferon-gamma (IFN-gamma) are considered to be important in the development of anaemia of chronic disease (ACD). (
  • Lackman RL, Cresswell P. Exposure of the promonocytic cell line THP-1 to Escherichia coli induces IFN-gamma-inducible lysosomal thiol reductase expression by inflammatory cytokines. (
  • The resistance to IL-1beta conferred by this procedure was stable, whereas the partial resistance to IFN-gamma was transient but reinducible by culture in the presence of cytokines. (
  • Gamma interferon (IFNγ), a type II IFN, was discovered approximately seven years after discovery of type I IFNs. (
  • Type I (alpha, beta) and type II (gamma) interferons (IFNs) can restrict the growth of many cell types. (
  • The interferons (IFNs) were identified first as antiviral agents. (
  • Objective- We previously reported that interferons (IFNs) regulate transcription of HIF-1α in human endothelial cells (ECs), linking immunity and hypoxia. (
  • It was demonstrated that interferon-gamma (IFN)-gamma can reverse immunoparalysis in vitro and in vivo in animals and in healthy volunteers. (
  • The target of gamma-IFN activity may be the infected hepatocytes themselves, as shown by in vitro experiments in which small doses of the human lymphokine inhibited the development of exoerythrocytic forms of Plasmodium berghei in a human hepatoma cell line. (
  • Both, inhibitory and stimulatory activities of IFN-gamma on erythropoiesis have been observed in vitro earlier. (
  • Recombinant gamma interferon could not replace the function of Th1 cells in vivo and had limited direct antiviral activity in vitro. (
  • Listeriosis in mice with the severe combined immunodeficiency (SCID) mutation is an established model in vivo and in vitro of interferon gamma (IFN-gamma)-dependent macrophage activation by natural killer (NK) cells during the development of natural immunity. (
  • An in vivo rodent model of retinal detachment has been used to measure the effect of interferon gamma on re-absorption following retinal detachment and showed that the addition of interferon gamma to the anterior eye surface caused a significant, rapid decrease in retinal detachment volume in the first hour of observation. (
  • This paper reports the effect of interferon gamma therapy in a single patient suffering from severe FRDA cardiomyopathy. (
  • Some side effects of interferon gamma-1b may occur that usually do not need medical attention . (
  • In the present study, we investigated the effects of interferon gamma, a potent immunomodulator derived from lymphocytes, on hypertension and organ damage in Dahl salt-sensitive rats and in spontaneously hypertensive rats. (
  • We have now utilized neutralizing monoclonal antibodies specific for murine IFN-gamma to investigate the mechanism of macrophage activation in scid mice. (
  • The flu-like side effects of interferon may be stressful to heart patients. (
  • INF-stimulated gene transcription, a key early event in IFN response, acts through the Janus kinase-signal transducers and activators of transcription pathway, in which both IFN-alpha and IFN-gamma activate the transcription factor Stat1. (
  • To determine the mechanism by which IFN-gamma affects IL-12-mediated GVHD protection, we have compared the effect of IL-12 on GVHD in C57BL/6 wild-type (WT) or IFN-gamma gene knockout (GKO) recipients of fully major histocompatibility complex plus minor antigen-mismatched allogeneic BMT from WT or GKO BALB/c mice. (
  • Whereas large amounts of data have been accumulated regarding IFN gamma gene expression in these two cell types, little information about IFN gamma expression in other cell types exists. (
  • Analysis of IFN gamma genomic DNA indicates that the gene is not amplified, but that hypomethylation in the 5′ noncoding region of the IFN gamma gene has occurred in the B-cell line from the Burkitt's lymphoma patient that spontaneously produces IFN gamma. (
  • This finding suggests that the methylation state of the promoter region may play an important role in the control of IFN gamma gene expression in B cells. (
  • Here we show that PRAME gene transcription in leukaemic cell lines is rapidly induced by exposure of cells to bacterial PAMPs (pathogen associated molecular patterns) in combination with type 2 interferon (IFNγ). (
  • IFN-gamma induces several biochemical pathways in human monocytes, among them neopterin formation by GTP-cyclohydrolase I (GTP-CH I) and tryptophan degradation by the enzyme indoleamine 2,3-dioxygenase (IDO). (
  • Moreover, in a case-series of septic patients interferon-gamma treatment leaded to reversal of immunoparalysis, reduction in mechanical ventilation time and length of stay with no relevant side-effects. (
  • IFN-gamma, in addition to having antiviral activity, has important immunoregulatory functions. (
  • It is a potent activator of macrophages, it has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons. (
  • IFN-gamma, in addition to having antiviral activity, has important immunoregulatory functions, it is a potent activator of macrophages, and has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons. (
  • A role for alpha/beta interferon (IFN-α/β) in the IFN-γ antiviral response has long been suggested. (
  • Neutralization of endogenous gamma interferon abrogated the antiviral activity of Th1 cells but not that of CD8+ T lymphocytes in other tissues. (
  • Smith D, Mayhew ER, Reszka M, Ito M, Omalley J (1990) Antiviral and antiproliferative properties of liposome-associated human interferon-gamma. (
  • Some scientific studies show that the interferon-gamma release assay is more accurate and correlates more highly with TB exposure as compared to the tuberculin skin test (TST). (
  • Interferon gamma-1b is also used to slow the progression of a bone disorder called malignant osteopetrosis. (
  • 1983. Interferon-like factors from antigen-and mitogen-stimulated human leukocytes with antirickettsial and cytolytic actions on Rickettsia prowazekii infected human endothelial cells, fibroblasts, and macrophages. (
  • recombinant human interferon-γ, expressed and purified from Escherichia coli . (
  • IFN-Gamma Human Recombinant produced in E.Coli is a single, glycosylated polypeptide chain containing 159 amino acids (24-161 a.a) without cysteine residues and having a molecular mass of 18.5kDa (confirmed by MALDI-TOF). (
  • A specific DNA probe was used to study the effect of recombinant rat, mouse, and human gamma-interferon (gamma-IFN) on the course of sporozoite-induced malaria infections. (
  • Human gamma-IFN also diminished the parasitemia in chimpanzees infected with sporozoites of the human malaria parasite Plasmodium vivax. (
  • Three-dimensional structure of recombinant human interferon-gamma. (
  • Therefore, IFN-gamma-mediated pathways were investigated in human CD34(+) progenitor cells, and effects of IFN-gamma on the proliferative activity of different progenitor subpopulations were studied. (
  • Gamma-interferon-inducible lysosomal thiol reductase is upregulated in human melanoma. (
  • Plasma samples obtained from patients receiving a 24-h continuous infusion of human recombinant (hr)TNF or a combination of two overlapping, 24-h continuous infusions of hrIFN-gamma and hrTNF were analyzed for IL-6 in a sensitive bioassay. (
  • Boyanova M, Tsanev R, Ivanov I (2002) A modified kynurenine bioassay for quantitative determination of human interferon-γ. (
  • Chen TL, Lin YL, Lee YL, Yang NS, Chan MT (2004) Expression of bioactive human interferon-gamma in transgenic rice cell suspension cultures. (
  • Derynck R, Singh A, Goeddel DV (1983) Expression of the human interferon-gamma cDNA in yeast. (
  • Dobrovolsky VN, Lagutin OV, Vinogradova TV, Frolova IS, Kuznetsov VP, Larionov OA (1993) Human gamma-interferon expression in the mammary gland of transgenic mice. (
  • Rinderknecht E, O'Connor BH, Rodriguez H (1984) Natural human interferon-gamma: complete amino acid sequence and determination of sites of glycosylation. (
  • Human IFN-gamma is highly species specific and is biologically active only in human and primate cells. (
  • In the present investigation we downregulated hTERT using cognate siRNA in two highly invasive human glioblastoma cell lines SNB-19 and LN-18 and simultaneously treated them with interferon-γ for 48 h. (
  • In this study, we have analyzed the production of IFN gamma by the Epstein- Barr virus (EBV)-positive B-cell line, JLP(c), derived from a patient with Burkitt's lymphoma, and another human B-cell line, PA682BM-1, which was derived from an acquired immunodeficiency syndrome patient. (
  • To confirm further the presence of IFN-gamma activity, these T cell supernatants were assayed for their ability to activate macrophages as measured by induction of nitrite production. (
  • We demonstrate that IFN-gamma production from SCID splenocytes is stimulated by interleukin (IL) 12, tumor necrosis factor alpha (TNF-alpha), and IL-2 but is inhibited by IL-10, IL-10, IL-12, and TNF are induced by heat-killed Listeria monocytogenes (hk-LM) from SCID splenocytes and peritoneal macrophages. (
  • Gamma-Interferon-inducible lysosomal thiol reductase (GILT) maintains phagosomal proteolysis in alternatively activated macrophages. (
  • Determine surrogate immunologic markers for response to interferon gamma, functional integrity and anti-fungal activity of phagocytic cells (neutrophils, monocytes, and macrophages), and nonphagocytic effector cells (natural killer and T cells) in these patients. (
  • Enhancement of growth of Mycobacterium lepraemurium in macrophages by gamma interferon. (
  • Cytotoxicity in glioma cells due to interleukin-12 and interleukin-18-stimulated macrophages mediated by interferon-gamma-regulated nitric oxide. (
  • Interferon gamma-1b injection comes as a solution to inject subcutaneously (just under the skin) three times a week, for example, every Monday, Wednesday, and Friday. (
  • Arm I: Patients receive voriconazole (IV over 80-120 minutes for the first 3 doses and orally every 12 hours for subsequent doses) 3 times per week and interferon gamma subcutaneously (SC) 3 times per week. (
  • ISGF3 gamma p48, a specificity switch for interferon activated transcription factors. (
  • beta interferon by fibroblasts, and gamma interferon by natural killer cells and cytotoxic T lymphocytes (killer T cells). (
  • Gamma interferon also stimulates natural killer cells. (
  • Production of interferon-gamma by chicken T cells. (
  • These results show that chicken T cells produce IFN-gamma. (
  • IL-6 mRNA production was increased in a dose-dependent fashion up to 15-fold in LPS-stimulated cells with the addition of IFN-gamma. (
  • The enhanced production of IL-6 mRNA that occurs with the addition of IFN-gamma to LPS-stimulated monocytic cells was due to increased transcription of IL-6 mRNA. (
  • Interferon gamma-1b can lower blood cells that help your body fight infections and help your blood to clot. (
  • IL-2R bearing cells were virtually absent as was anti-HLA-DR reactivity of the keratinocytes, underlining a defective intra-lesional interferon-gamma activity. (
  • Only cells in which complete Stat1 activity was available (Stat1alpha) were growth-inhibited by IFN-gamma. (
  • In mice and rats infected with sporozoites of Plasmodium berghei, mouse and rat gamma-IFN's strongly inhibited the development of the exoerythrocytic forms in the liver liver cells of the hosts, but not the development of the erythrocytic stages. (
  • Interferon gamma may increase the number of adhesion molecules on white blood cells, and thus improve their function. (
  • At these same time intervals, patients will provide a salt-water mouth rinse specimen, which will be tested for changes in the number of white blood cells during interferon gamma treatment. (
  • Stimulation of CD34(+) cells with IFN-gamma in different doses (either 5000U/ml once or 200 and 400U/ml every other day) induced tryptophan degradation and in parallel also neopterin formation. (
  • IFN-gamma stimulated higher kynurenine and neopterin formation in cells cultivated in EGFCM, stimulation with 400U IFN-gamma every other day was most effective. (
  • In conclusion, stimulation of haematopoietic stem cells with IFN-gamma activates IDO and neopterin formation, and it also exerts an influence on the proliferation of various stem cell populations. (
  • The ability of T cells to induce these responses has been extensively documented and occurs via their secretion of interferon-gamma (IFN-gamma) after interaction with antigen (2-6). (
  • Interleukin 12 and tumor necrosis factor alpha are costimulators of interferon gamma production by natural killer cells in severe combined immunodeficiency mice with listeriosis, and interleukin 10 is a physiologic antagonist. (
  • Thus, these data indicate that macrophage production of TNF-alpha and IL-12 stimulates the release of IFN-gamma by NK cells and that IL-10 produced in response to hk-LM inhibits this response at the level of the macrophage and the NK cell. (
  • Cutting edge: induction of the antigen-processing enzyme IFN-gamma-inducible lysosomal thiol reductase in melanoma cells is STAT1-dependent but CIITA-independent. (
  • To this end, we have cultured INS-1 insulinoma cells in increasing concentrations of interleukin-1beta (IL-1beta) + gamma-interferon (IFN-gamma), with approximate weekly iterations over an 8-week period. (
  • These cells were also 78 +/- 1.2% viable after 5 days of exposure to the combination of 10 ng/ml IL-1beta and 100 U/ml IFN-gamma, whereas parental INS-1 cells treated in the same manner were only 0.3 +/- 0.03% viable. (
  • The present study highlights the beneficial effects of a novel BiPPB mediated cell-specific targeting of IFN gamma peptidomimetic to the disease-inducing cells and therefore represents a highly potential therapeutic approach to treat fibrotic diseases. (
  • Both the IgE-enhancing activity and IFN-gamma act directly upon purified B cells. (
  • Taken together, the results of the present study indicate that the combination treatment of hTERT siRNA and interferon-γ in glioblastoma cells effectively prevents cell invasion, angiogenesis, and tumor growth through downregulation of molecules involved in angiogenesis and cell cycle. (
  • Multifunctional CD4 Cells Expressing Gamma Interferon and Perforin Med" by Deborah M. Brown, Sarah Lee et al. (
  • This pilot study will investigate the safety, tolerability and potential efficacy of serial ocular instillations of topical interferon gamma-1b for classic CSC. (
  • Design: In this Phase I/II, non-randomized, prospective, uncontrolled, dose-escalation, single-center pilot study, a series of ocular instillations of topical interferon gamma-1b will be administered in the study eye over a two-week period. (
  • Amarillo Biosciences, Inc., (ABI), is a biopharmaceutical company developing low-dose oral and topical interferon alpha. (
  • The keratinocytes covering tuberculoid lesions abundantly expressed class II alloantigens (HLA-DR antigens), indicating high intra-lesional interferon-gamma activity. (
  • IFN-gamma produced by lymphocytes activated by specific antigens or mitogens. (
  • In contrast, IL-10 inhibits hk-LM-induced IFN-gamma production at two levels: (i) by inhibiting TNF and IL-12 production from these cultures (presumably from the macrophage) and (ii) by inhibiting the stimulatory effects of IL-12 and TNF-alpha on NK-cell IFN-gamma production. (
  • OBJECTIVE: As Mycobacterium tuberculosis isolation rates in tuberculous effusions are relatively low, several biochemical and immunological markers have been proposed to diagnose tuberculous pleurisy including adenosine deaminase (ADA) and interferon-gamma (IFN-γ). (
  • Recently, it has been shown that in vivo administration of interferon gamma (IFN-gamma) upregulates CD18 expression in normals and alters leukocyte trafficking. (
  • A selected defect of γ-interferon secretion [3] and the partial effect of γ-interferon in a patient with the X-linked lymphoproliferative syndrome [4] have been previously reported. (
  • Interferons help the body fight viral infections. (
  • Interferon gamma-1b is used to prevent serious infections in people with a condition called chronic granulomatous disease. (
  • Determine the safety profile of voriconazole and interferon gamma in patients with invasive aspergillosis or other filamentous fungal infections. (
  • Here are the most relevant search results for "Treatment Of Non-Tuberculous Mycobacterial Infections With Interferon Gamma" found in our extensive corporate database of over 50,000 company records. (
  • All interferons inhibit viral replication by interfering with the transcription of viral nucleic acid. (
  • Interferon activation of the transcription factor Stat91 involves dimerization through SH2-phosphotyrosyl peptide interactions. (
  • Founded in 1983, GAMMA has privileged access to the University of Liège and is active in technology transfer from research laboratories to commercial applications. (
  • Those reports also contained the basic observation underlying the now widely employed interferon gamma release assay used to test for tuberculosis. (
  • These interferons have been classified into two types: type I includes the alpha and beta forms, and type II consists of the gamma form. (
  • In mammals, interferon (IFN)-alpha/beta (type I) is typically resistant to exposure to heat and low pH, whereas IFN-gamma (type II) is labile. (
  • Belongs to the type II (or gamma) interferon family. (
  • It binds interferon-γ, the sole member of interferon type II. (
  • Accordingly, IFN-gamma should boost autoimmune diseases of the Th1 type. (
  • Together with the previously demonstrated type III interferon response, these results show that the counterparts of the mammalian viral and bacterial interferon-dependent defence functions are in place in zebrafish embryos, and suggest that zebrafish IFN-γ1 and IFN-γ2 are functionally equivalent to mammalian IFN-γ. (
  • Interferon-γ may prove useful in treating a different set of diseases-for example, chronic conditions such as rheumatoid arthritis. (
  • It is not known exactly how interferon gamma-1b works to treat chronic granulomatous disease and osteopetrosis. (
  • Interferon gamma is used to treat chronic granulomatous disease and severe osteoporosis. (
  • Recombinant interferon-gamma (rIFN-gamma) therapy has become an effective form of prophylaxis for patients with chronic granulomatous disease (CGD). (
  • Compare the efficacy and possible heterogeneity in efficacy of voriconazole with or without interferon gamma across different patient sub-populations, in terms of designing a larger phase II or pivotal phase III study. (
  • Inject interferon gamma-1b injection at around the same time of day each time you inject it. (
  • Use interferon gamma-1b injection exactly as directed. (
  • tell your doctor and pharmacist if you are allergic to interferon gamma-1b injection, products made from the E. coli bacteria, any other medications, or any of the other ingredients in interferon gamma-1b injection. (
  • If you become pregnant while receiving interferon gamma-1b injection, call your doctor. (
  • If you miss a dose of interferon gamma-1b injection, do not increase your dose or give two injections to make up for the missed dose.Call your doctor if you miss a dose and have questions about what to do. (
  • Interferon gamma-1b injection may cause side effects. (
  • Subcutaneous injection of interferon gamma (5 x 10(4) units/kg body wt once a week for 10 weeks) reduced blood pressure in Dahl salt-sensitive rats fed a 4% high salt diet (174 versus 194 mm Hg, p less than 0.025). (
  • Interferon Sciences' ALFERON N Injection(R) (Interferon Alfa-n3) product has been approved by the United States Food and Drug Administration ("FDA. (
  • Now, a study published in the July 2016 issue of Nature from a research team lead by Dr. Vladimir Litvak at the University of Massachusetts and Dr. Jonathan Kipnis at the University of Virginia demonstrates a critical role for meningeal interferon-γ (IFN-γ) in mediating both neural function and social behavior ( Filiano et al, 2016 ). (
  • Usage information: Donor-derived interferon gamma is required for inhibition of acute graft-versus-host disease by interleukin 12. (
  • Donor-derived interferon gamma is required for inhibition of acute graft-versus-host disease by interleukin 12. (
  • recombinant rat interferon-γ expressed in E. coli. (
  • The degree of inhibition of the exoerythrocytic forms was proportional to the dose of gamma-IFN administered, but was independent of the number of sporozoites used for challenge. (
  • Moreover, the effective inhibition of host-reactive donor T cell activation and expansion that is associated with IL-12-mediated GVHD protection was dependent on the ability of BALB/c donors to produce IFN-gamma. (
  • Gamma interferon-induced, nitric oxide-mediated inhibition of vaccinia virus replication. (
  • A T cell activity that enhances polyclonal IgE production and its inhibition by interferon-gamma. (
  • Matrigel invasion, spheroid migration, and cell proliferation studies demonstrated inhibition of cell invasion, migration, and proliferation in both glioblastoma cell lines after treatment with hTERT siRNA and interferon-γ. (
  • Maric M, Barjaktarevic I, Bogunovic B, Stojakovic M, Maric C, Vukmanovic S. Cutting edge: developmental up-regulation of IFN-gamma-inducible lysosomal thiol reductase expression leads to reduced T cell sensitivity and less severe autoimmunity. (
  • You should not use this medicine if you are allergic to interferon gamma-1b, or to drug products made from E. coli bacteria. (
  • Transcriptional induction of IFN-gamma-responsive genes is modulated by DNA surrounding the interferon stimulation response element. (
  • A study of genes associated with an effective host response by Chinook salmon ( Oncorhynchus tshawytscha ) to a persistent Gram-positive bacterial pathogen ( Renibacterium salmoninarum ) suggested that an interferon-inducible signal was abrogated during early stages of exposure. (
  • As in the induction of target genes, the two interferons act at least partly redundantly. (