A DNA-dependent RNA polymerase present in bacterial, plant, and animal cells. It functions in the nucleoplasmic structure where it transcribes DNA into RNA. It has specific requirements for cations and salt and has shown an intermediate sensitivity to alpha-amanitin in comparison to RNA polymerase I and II. EC 2.7.7.6.
One of several general transcription factors that are specific for RNA POLYMERASE III. TFIIIB recruits and positions pol III over the initiation site and remains stably bound to the DNA through multiple rounds of re-initiation by RNA POLYMERASE III.
Product of the retinoblastoma tumor suppressor gene. It is a nuclear phosphoprotein hypothesized to normally act as an inhibitor of cell proliferation. Rb protein is absent in retinoblastoma cell lines. It also has been shown to form complexes with the adenovirus E1A protein, the SV40 T antigen, and the human papilloma virus E7 protein.
Factors that bind to RNA POLYMERASE III and aid in transcription. They include the assembly factors TFIIIA and TFIIIC and the initiation factor TFIIIB. All combine to form a preinitiation complex at the promotor that directs the binding of RNA POLYMERASE III.
The biosynthesis of RNA carried out on a template of DNA. The biosynthesis of DNA from an RNA template is called REVERSE TRANSCRIPTION.
A DNA-dependent RNA polymerase present in bacterial, plant, and animal cells. It functions in the nucleoplasmic structure and transcribes DNA into RNA. It has different requirements for cations and salt than RNA polymerase I and is strongly inhibited by alpha-amanitin. EC 2.7.7.6.
A malignant tumor arising from the nuclear layer of the retina that is the most common primary tumor of the eye in children. The tumor tends to occur in early childhood or infancy and may be present at birth. The majority are sporadic, but the condition may be transmitted as an autosomal dominant trait. Histologic features include dense cellularity, small round polygonal cells, and areas of calcification and necrosis. An abnormal pupil reflex (leukokoria); NYSTAGMUS, PATHOLOGIC; STRABISMUS; and visual loss represent common clinical characteristics of this condition. (From DeVita et al., Cancer: Principles and Practice of Oncology, 5th ed, p2104)
The sum of the weight of all the atoms in a molecule.
Heparin fractions with a molecular weight usually between 4000 and 6000 kD. These low-molecular-weight fractions are effective antithrombotic agents. Their administration reduces the risk of hemorrhage, they have a longer half-life, and their platelet interactions are reduced in comparison to unfractionated heparin. They also provide an effective prophylaxis against postoperative major pulmonary embolism.
Occupations of medical personnel who are not physicians, and are qualified by special training and, frequently, by licensure to work in supporting roles in the health care field. These occupations include, but are not limited to, medical technology, physical therapy, physician assistant, etc.
Ribonucleic acid in bacteria having regulatory and catalytic roles as well as involvement in protein synthesis.
Development of a library collection, including the determination and coordination of selection policy, assessment of needs of users and potential users, collection use studies, collection evaluation, identification of collection needs, selection of materials, planning for resource sharing, collection maintenance and weeding, and budgeting.
A publication issued at stated, more or less regular, intervals.
Individual's rights to obtain and use information collected or generated by others.
A quantitative measure of the frequency on average with which articles in a journal have been cited in a given period of time.
The use of statistical methods in the analysis of a body of literature to reveal the historical development of subject fields and patterns of authorship, publication, and use. Formerly called statistical bibliography. (from The ALA Glossary of Library and Information Science, 1983)
"The business or profession of the commercial production and issuance of literature" (Webster's 3d). It includes the publisher, publication processes, editing and editors. Production may be by conventional printing methods or by electronic publishing.
The evaluation by experts of the quality and pertinence of research or research proposals of other experts in the same field. Peer review is used by editors in deciding which submissions warrant publication, by granting agencies to determine which proposals should be funded, and by academic institutions in tenure decisions.
Proteins which maintain the transcriptional quiescence of specific GENES or OPERONS. Classical repressor proteins are DNA-binding proteins that are normally bound to the OPERATOR REGION of an operon, or the ENHANCER SEQUENCES of a gene until a signal occurs that causes their release.
An essential ribonucleoprotein reverse transcriptase that adds telomeric DNA to the ends of eukaryotic CHROMOSOMES.
A terminal section of a chromosome which has a specialized structure and which is involved in chromosomal replication and stability. Its length is believed to be a few hundred base pairs.
A genus of ciliate protozoa commonly used in genetic, cytological, and other research.
A polynucleotide consisting essentially of chains with a repeating backbone of phosphate and ribose units to which nitrogenous bases are attached. RNA is unique among biological macromolecules in that it can encode genetic information, serve as an abundant structural component of cells, and also possesses catalytic activity. (Rieger et al., Glossary of Genetics: Classical and Molecular, 5th ed)
A species of ciliate protozoa used in genetic and cytological research.
Catalytically active enzymes that are formed by the combination of an apoenzyme (APOENZYMES) and its appropriate cofactors and prosthetic groups.
A species of ciliate protozoa used extensively in genetic research.
Enzymes that catalyze DNA template-directed extension of the 3'-end of an RNA strand one nucleotide at a time. They can initiate a chain de novo. In eukaryotes, three forms of the enzyme have been distinguished on the basis of sensitivity to alpha-amanitin, and the type of RNA synthesized. (From Enzyme Nomenclature, 1992).
The state of weariness following a period of exertion, mental or physical, characterized by a decreased capacity for work and reduced efficiency to respond to stimuli.
Databases containing information about PROTEINS such as AMINO ACID SEQUENCE; PROTEIN CONFORMATION; and other properties.
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
A process that includes the determination of AMINO ACID SEQUENCE of a protein (or peptide, oligopeptide or peptide fragment) and the information analysis of the sequence.
DNA-dependent DNA polymerases found in bacteria, animal and plant cells. During the replication process, these enzymes catalyze the addition of deoxyribonucleotide residues to the end of a DNA strand in the presence of DNA as template-primer. They also possess exonuclease activity and therefore function in DNA repair.
Software used to locate data or information stored in machine-readable form locally or at a distance such as an INTERNET site.
Databases devoted to knowledge about specific genes and gene products.
A species of temperate bacteriophage in the genus P2-like viruses, family MYOVIRIDAE, which infects E. coli. It consists of linear double-stranded DNA with 19-base sticky ends.
The complete genetic complement contained in the DNA of a set of CHROMOSOMES in a HUMAN. The length of the human genome is about 3 billion base pairs.
The 9th cranial nerve. The glossopharyngeal nerve is a mixed motor and sensory nerve; it conveys somatic and autonomic efferents as well as general, special, and visceral afferents. Among the connections are motor fibers to the stylopharyngeus muscle, parasympathetic fibers to the parotid glands, general and taste afferents from the posterior third of the tongue, the nasopharynx, and the palate, and afferents from baroreceptors and CHEMORECEPTOR CELLS of the carotid sinus.
Immunoglobulin molecules having a specific amino acid sequence by virtue of which they interact only with the ANTIGEN (or a very similar shape) that induced their synthesis in cells of the lymphoid series (especially PLASMA CELLS).
A loose confederation of computer communication networks around the world. The networks that make up the Internet are connected through several backbone networks. The Internet grew out of the US Government ARPAnet project and was designed to facilitate information exchange.
Elements that are transcribed into RNA, reverse-transcribed into DNA and then inserted into a new site in the genome. Long terminal repeats (LTRs) similar to those from retroviruses are contained in retrotransposons and retrovirus-like elements. Retroposons, such as LONG INTERSPERSED NUCLEOTIDE ELEMENTS and SHORT INTERSPERSED NUCLEOTIDE ELEMENTS do not contain LTRs.
Nucleotide sequences repeated on both the 5' and 3' ends of a sequence under consideration. For example, the hallmarks of a transposon are that it is flanked by inverted repeats on each end and the inverted repeats are flanked by direct repeats. The Delta element of Ty retrotransposons and LTRs (long terminal repeats) are examples of this concept.
Insertion of viral DNA into host-cell DNA. This includes integration of phage DNA into bacterial DNA; (LYSOGENY); to form a PROPHAGE or integration of retroviral DNA into cellular DNA to form a PROVIRUS.
The material of CHROMOSOMES. It is a complex of DNA; HISTONES; and nonhistone proteins (CHROMOSOMAL PROTEINS, NON-HISTONE) found within the nucleus of a cell.
DNA sequences which are recognized (directly or indirectly) and bound by a DNA-dependent RNA polymerase during the initiation of transcription. Highly conserved sequences within the promoter include the Pribnow box in bacteria and the TATA BOX in eukaryotes.
A large family of RNA helicases that share a common protein motif with the single letter amino acid sequence D-E-A-D (Asp-Glu-Ala-Asp). In addition to RNA helicase activity, members of the DEAD-box family participate in other aspects of RNA metabolism and regulation of RNA function.
Interferon secreted by leukocytes, fibroblasts, or lymphoblasts in response to viruses or interferon inducers other than mitogens, antigens, or allo-antigens. They include alpha- and beta-interferons (INTERFERON-ALPHA and INTERFERON-BETA).
An interferon regulatory factor that is expressed constitutively and undergoes POST-TRANSLATIONAL MODIFICATION following viral infection. PHOSPHORYLATION of IRF-3 causes the protein to be translocated from the CYTOPLASM to CELL NUCLEUS where it binds DNA, and activates transcription.
One of the type I interferons produced by fibroblasts in response to stimulation by live or inactivated virus or by double-stranded RNA. It is a cytokine with antiviral, antiproliferative, and immunomodulating activity.
The capacity of a normal organism to remain unaffected by microorganisms and their toxins. It results from the presence of naturally occurring ANTI-INFECTIVE AGENTS, constitutional factors such as BODY TEMPERATURE and immediate acting immune cells such as NATURAL KILLER CELLS.
The intracellular transfer of information (biological activation/inhibition) through a signal pathway. In each signal transduction system, an activation/inhibition signal from a biologically active molecule (hormone, neurotransmitter) is mediated via the coupling of a receptor/enzyme to a second messenger system or to an ion channel. Signal transduction plays an important role in activating cellular functions, cell differentiation, and cell proliferation. Examples of signal transduction systems are the GAMMA-AMINOBUTYRIC ACID-postsynaptic receptor-calcium ion channel system, the receptor-mediated T-cell activation pathway, and the receptor-mediated activation of phospholipases. Those coupled to membrane depolarization or intracellular release of calcium include the receptor-mediated activation of cytotoxic functions in granulocytes and the synaptic potentiation of protein kinase activation. Some signal transduction pathways may be part of larger signal transduction pathways; for example, protein kinase activation is part of the platelet activation signal pathway.
The type species of RESPIROVIRUS in the subfamily PARAMYXOVIRINAE. It is the murine version of HUMAN PARAINFLUENZA VIRUS 1, distinguished by host range.
Chu WM, Wang Z, Roeder RG, Schmid CW (1997). "RNA polymerase III transcription repressed by Rb through its interactions with ... of human TFIIIC and their involvement in functional interactions with TFIIIB and RNA polymerase III". Mol. Cell. Biol. 19 (7): ... of human TFIIIC and their involvement in functional interactions with TFIIIB and RNA polymerase III". Mol. Cell. Biol. 19 (7): ... Innes F, Ramsbottom B, White RJ (2006). "A test of the model that RNA polymerase III transcription is regulated by selective ...
"RNA polymerase III transcription repressed by Rb through its interactions with TFIIIB and TFIIIC2". J. Biol. Chem. 272 (23): ... Wang Z, Roeder RG (1997). "Three human RNA polymerase III-specific subunits form a subcomplex with a selective function in ... "Entrez Gene: BDP1 B double prime 1, subunit of RNA polymerase III transcription initiation factor IIIB". Chu WM, Wang Z, Roeder ... Hu P, Wu S, Hernandez N (2003). "A minimal RNA polymerase III transcription system from human cells reveals positive and ...
Hu P, Wu S, Hernandez N (Dec 2004). "A role for beta-actin in RNA polymerase III transcription". Genes & Development. 18 (24): ... P166A: interaction zone between actin monomers. A333P: actin-myosin interaction zone. Pathogenesis appears to involve a ... Actin takes part in the regulation of chromatin structure, interacting with RNA polymerase I, II and III. In Pol I ... "Actin is part of pre-initiation complexes and is necessary for transcription by RNA polymerase II". Nature Cell Biology. 6 (11 ...
Acker J, de Graaff M, Cheynel I, Khazak V, Kedinger C, Vigneron M (Jul 1997). "Interactions between the human RNA polymerase II ... This gene encodes the third largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in ... interactions between two members of the TET family, EWS and hTAFII68, and subunits of TFIID and RNA polymerase II complexes". ... "Specific binding of RNA polymerase II to the human immunodeficiency virus trans-activating region RNA is regulated by cellular ...
RNA polymerase III, IFI16, have also been shown to activate STING through direct or indirect interactions. Cyclic GMP-AMP ... Several DNA sensors, such as DAI, RNA polymerase III, IFI16, DDX41 and cGAS, can detect foreign nucleic acids. After ... Adenovirus, herpes simplex virus, HSV-1 and HSV-2, as well as the negative-stranded RNA virus, vesicular stomatitis virus (VSV ... The hydrophobic residues from each STING protomer form hydrophobic interactions between each other at the interface. STING is ...
In Sen1, the N-terminus has shown interactions with the C-terminal domain of RNA polymerase II, ribonuclease III, and NER ... Senataxin interacts with RNA polymerase II and poly(A) binding proteins. At the C-terminal, senataxin has a DEAD box helicase ... The R-loops are thought to be involved in transcription termination by stalling RNA polymerase II. The senataxin protein, which ... June 2004). "DNA/RNA helicase gene mutations in a form of juvenile amyotrophic lateral sclerosis (ALS4)". American Journal of ...
Chu WM, Wang Z, Roeder RG, Schmid CW (1997). "RNA polymerase III transcription repressed by Rb through its interactions with ... This gene encodes one of the three subunits of the RNA polymerase III transcription factor complex. This complex plays a ... Wang Z, Roeder RG (1997). "Three human RNA polymerase III-specific subunits form a subcomplex with a selective function in ... of human TFIIIC and their involvement in functional interactions with TFIIIB and RNA polymerase III". Mol. Cell. Biol. 19 (7): ...
... of human TFIIIC and their involvement in functional interactions with TFIIIB and RNA polymerase III". Mol. Cell. Biol. 19 (7): ... of human TFIIIC and their involvement in functional interactions with TFIIIB and RNA polymerase III". Mol. Cell. Biol. 19 (7): ... "The TFIIIC90 subunit of TFIIIC interacts with multiple components of the RNA polymerase III machinery and contains a histone- ... 127 (3): 635-48. doi:10.1016/j.cell.2006.09.026. PMID 17081983. S2CID 7827573. Beausoleil SA, Jedrychowski M, Schwartz D, Elias ...
... of human TFIIIC and their involvement in functional interactions with TFIIIB and RNA polymerase III". Mol. Cell. Biol. 19 (7): ... "The TFIIIC90 subunit of TFIIIC interacts with multiple components of the RNA polymerase III machinery and contains a histone- ... "The TFIIIC90 subunit of TFIIIC interacts with multiple components of the RNA polymerase III machinery and contains a histone- ... 474 (7351): 262-3. doi:10.1038/474262a. PMID 21677718. Collins FS, Rossant J, Wurst W (2007). "A mouse for all reasons". Cell. ...
... interacting with RNA polymerase I,[110] II[108] and III.[109] In Pol I transcription, actin and myosin (MYO1C, which binds DNA ... This movement requires the interaction of actin and myosin.[127][128]. *Integration of different cellular compartments. Actin ... "A role for beta-actin in RNA polymerase III transcription". Genes & Development. 18 (24): 3010-3015. doi:10.1101/gad.1250804. ... "Actin is part of pre-initiation complexes and is necessary for transcription by RNA polymerase II". Nature Cell Biology. 6 (11 ...
... s are retrotransposons and look like DNA copies made from RNA polymerase III-encoded RNAs. Alu elements do not ... Alu element replication and mobilization begins by interactions with signal recognition particles (SRPs), which aid newly ... "Identification of RNA polymerase III-transcribed Alu loci by computational screening of RNA-Seq data". Nucleic Acids Research. ... which are transcribed by RNA polymerase III, have a similar but stronger promoter structure. Both boxes are located in the left ...
PC4 can interact with human TFIIIC to promote both accurate termination and transcription reinitiation by RNA polymerase III". ... Holloway AF، Occhiodoro F، Mittler G، Meisterernst M، Shannon MF (July 2000). "Functional interaction between the HIV ... transcriptional activator activity, RNA polymerase II distal enhancer sequence-specific binding. • ربط بروتيني. • RNA binding. ... transcription from RNA polymerase II promoter. • SMAD protein signal transduction. • transcription, DNA-templated. • positive ...
"The alpha-like RNA polymerase II core subunit 3 (RPB3) is involved in tissue-specific transcription and muscle differentiation ... Onions J, Hermann S, Grundström T (April 2000). "A novel type of calmodulin interaction in the inhibition of basic helix-loop- ... "The alpha-like RNA polymerase II core subunit 3 (RPB3) is involved in tissue-specific transcription and muscle differentiation ... Langlands K, Yin X, Anand G, Prochownik EV (August 1997). "Differential interactions of Id proteins with basic-helix-loop-helix ...
Acker J, de Graaff M, Cheynel I, Khazak V, Kedinger C, Vigneron M (July 1997). "Interactions between the human RNA polymerase ... This gene encodes a subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. The ... "Specific binding of RNA polymerase II to the human immunodeficiency virus trans-activating region RNA is regulated by cellular ... DNA-directed RNA polymerase II subunit RPB11-a is an enzyme that in humans is encoded by the POLR2J gene. ...
Examples: Protein complexes, some of which are multienzyme complexes: proteasome, DNA polymerase III holoenzyme, RNA polymerase ... RNA, DNA, carbohydrate) or large non-polymeric biomolecules (lipid). The interactions between these biomolecules are non- ... via charge-charge interactions, van der Waals forces, and dipole-dipole interactions such as hydrogen bonds), by definition MAs ... Dominance interactions in Escherichia coli cells mixedly infected with bacteriophage T4D wild-type and amber mutants and their ...
... directly influence gene transcription as it interacts with chromatin remodeling complexes as well as RNA polymerases I, II and ... Therefore, further exploration of laminin-111 and nuclear actin interaction could be a target for future experimental ... III. However, the exact role that actin plays in transcription has not yet been determined. A group of distinguished scientists ... It has been suggested that actin interactions ...
... is directly recruited to the TATA box of polymerase III small nuclear RNA gene promoters through its interaction with the TATA- ... is directly recruited to the TATA-box of polymerase III small nuclear RNA gene promoters through its interaction with TATA- ... 1999). "RNA polymerase III transcription factor IIIB is a target for repression by pocket proteins p107 and p130". Mol. Cell. ... This gene encodes one of the multiple subunits of the RNA polymerase III transcription factor complex required for ...
Pan G, Aso T, Greenblatt J (1997). "Interaction of elongation factors TFIIS and elongin A with a human RNA polymerase II ... Ramanathan Y, Rajpara SM, Reza SM, Lees E, Shuman S, Mathews MB, Pe'ery T (2001). "Three RNA polymerase II carboxyl-terminal ... Mediator of RNA polymerase II transcription subunit 21 is an enzyme that in humans is encoded by the MED21 gene. MED21 has been ... Cho H, Orphanides G, Sun X, Yang XJ, Ogryzko V, Lees E, Nakatani Y, Reinberg D (Sep 1998). "A human RNA polymerase II complex ...
This sequence of RNA is used as a primer to initiate DNA polymerase III. The RNA bases are ultimately replaced with DNA bases ... Assembly is initiated by interactions of PriA and PriB with ssDNA and the pas. PriC, DnaB, DnaC, and DnaT then act on the ... The primosome attaches 1-10 RNA nucleotides to the single stranded DNA creating a DNA-RNA hybrid. ... In molecular biology, a primosome is a protein complex responsible for creating RNA primers on single stranded DNA during DNA ...
... and described the cascade of protein-DNA and protein-protein interaction leading to the recruitment of RNA polymerase III on ... Eukaryotic RNA polymerases », CRC Crit. Rev. Biochem., (1985), 18, p. 31-90 Chédin, S., et al., « The yeast RNA polymerase III ... He gave the first complete description of the three forms of nuclear RNA polymerases which are composed of the assembly of many ... EMBO J. (1996) 15: 1941-1949 Dieci, G. and Sentenac, A. Facilitated recycling pathway for RNA polymerase III. Cell (1996) 84: ...
Noel RJ, Reznikoff WS (10 March 2000). "Structural Studies of lacUV5-RNA Polymerase Interactions in Vitro". Journal of ... Due to this, lacUV5 recruits RNA Polymerase more effectively, thus leading to higher transcription of target genes. ... Pribnow, D (1975). "Bacteriophage T7 Early Promoters: Nucleotide Sequences of Two RNA Polymerase Binding Sites". Journal of ... "Use of bacteriophage T7 RNA polymerase to direct selective high-level expression of cloned genes". Journal of Molecular Biology ...
"Characterization of human RNA polymerase III identifies orthologues for Saccharomyces cerevisiae RNA polymerase III subunits" ( ... "A human protein-protein interaction network: a resource for annotating the proteome". Cell. 122 (6): 957-68. doi:10.1016/j.cell ... The protein encoded by this gene is one of more than a dozen subunits forming eukaryotic RNA polymerase III (RNA Pol III), ... two subunits of RNA polymerase III transcription initiation factor IIIB (TFIIIB). Unlike most of the other RNA Pol III subunits ...
Plaschka, Clemens; Nozawa, Kayo; Cramer, Patrick (2016). "Mediator Architecture and RNA Polymerase II Interaction". Journal of ... three-dimensional structure of RNA polymerase II, one of the biggest enzymes in the cell nucleus. This work played a decisive ... Cheung, Alan C.M.; Cramer, Patrick (2012). "A Movie of RNA Polymerase II Transcription". Cell. 149 (7): 1431-1437. doi:10.1016/ ... Cheung, AC; Cramer, Patrick (2012). "A Movie of RNA polymerase II Transcription". Cell. 149 (7): 1431-1437. doi:10.1016/j.cell. ...
RNA polymerase III (Pol III) transcribes small non-coding RNAs, including tRNAs, 5S rRNA, U6 snRNA, SRP RNA, and other stable ... Crystal structures of RNA polymerases I and II provide an opportunity to understand the interactions among the subunits and the ... Eukaryotes have three nuclear RNA polymerases, each with distinct roles and properties. RNA polymerase I (Pol I) catalyses the ... RNA Polymerases I, II, and III contain 14, 12, and 17 subunits, respectively. All three eukaryotic polymerases have five core ...
"Interactions between the human RNA polymerase II subunits". J. Biol. Chem. UNITED STATES. 272 (27): 16815-21. doi:10.1074/jbc. ... This gene encodes the second largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in ... "Specific binding of RNA polymerase II to the human immunodeficiency virus trans-activating region RNA is regulated by cellular ... "Entrez Gene: POLR2B polymerase (RNA) II (DNA directed) polypeptide B, 140kDa". Acker, J; de Graaff M; Cheynel I; Khazak V; ...
... interactions between two members of the TET family, EWS and hTAFII68, and subunits of TFIID and RNA polymerase II complexes". ... "The TFIIIC90 subunit of TFIIIC interacts with multiple components of the RNA polymerase III machinery and contains a histone- ... interactions between two members of the TET family, EWS and hTAFII68, and subunits of TFIID and RNA polymerase II complexes". ... "Entrez Gene: TAF5 TAF5 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 100kDa". Tao Y, Guermah M, Martinez ...
Eukaryotic transcription Post-transcriptional modification RNA polymerase I RNA polymerase II holoenzyme RNA polymerase III ... Many subunit-subunit interactions are known. DNA-directed RNA polymerase II subunit RPB1 - an enzyme that in humans is encoded ... The CTD domain does not exist in RNA Polymerase I or RNA Polymerase III. The RNA Polymerase CTD was discovered first in the ... RNA polymerase II holoenzyme is a form of eukaryotic RNA polymerase II that is recruited to the promoters of protein-coding ...
The B2 RNA is a small noncoding RNA polymerase III transcript that represses mRNA transcription in response to heat shock in ... 6S RNA, specifically associates with RNA polymerase holoenzyme containing the sigma70 specificity factor. This interaction ... VegT RNA, Oskar RNA, ENOD40, p53 RNA and SR1 RNA. Bifunctional RNAs have recently been the subject of a special issue of ... "Sequence analysis of RNase MRP RNA reveals its origination from eukaryotic RNase P RNA". RNA. 12 (5): 699-706. doi:10.1261/rna. ...
PC4 can interact with human TFIIIC to promote both accurate termination and transcription reinitiation by RNA polymerase III". ... Holloway AF, Occhiodoro F, Mittler G, Meisterernst M, Shannon MF (July 2000). "Functional interaction between the HIV ... Activated RNA polymerase II transcriptional coactivator p15 also known as positive cofactor 4 (PC4) or SUB1 homolog is a ... Malik S, Guermah M, Roeder RG (March 1998). "A dynamic model for PC4 coactivator function in RNA polymerase II transcription". ...
In addition it blocks RNA polymerase I transcription by binding to the DNA. DEDD2 (FLAME-3) is a DEDD homologue that shares a ... These interaction is defined by α1/α4 of procapase-8 DED-A and FADD DED α2/α3 or α1/α4 of FADD DED and α2/α5 of procapase-8 DED ... These interactions are defined by helices α1/α4 and α2/α3: residues Ser1, Val6, His9, Leu43, Asp44 and Glu51 from α1/α4 are in ... Each interaction involves an area of 1062 Å2 and contributions from hydrophobic side chains, hydrogen bonding and salt bridges ...
RNA polymerase II transcription regulatory region sequence-specific binding. • RNA polymerase II transcription factor activity ... negative regulation of transcription from RNA polymerase II promoter. • positive regulation of transcription from RNA ... RNA polymerase II regulatory region sequence-specific DNA binding. • DNA binding. • sequence-specific DNA binding. • ... polymerase II promoter. • skeletal system morphogenesis. • transcription, DNA-templated. • transcription from RNA polymerase II ...
... which is related to RNA polymerases found in bacteria. Chloroplasts also contain a mysterious second RNA polymerase that is ... The inverted repeat regions usually contain three ribosomal RNA and two tRNA genes, but they can be expanded or reduced to ... Sandelius AS (2009). The Chloroplast: Interactions with the Environment. Springer. p. 18. ISBN 978-3-540-68696-5. .. ... The two RNA polymerases may recognize and bind to different kinds of promoters within the chloroplast genome.[35] The ribosomes ...
RNA polymerase II core promoter sequence-specific DNA binding. • RNA polymerase II transcription factor activity, sequence- ... InteractionsEdit. PAX8 has been shown to interact with NK2 homeobox 1.[16] ... "Distinct functional properties of three human paired-box-protein, PAX8, isoforms generated by alternative splicing in thyroid, ... transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding. • RNA polymerase ...
RNA polymerase II core promoter proximal region sequence-specific binding. • metal ion binding. • RNA polymerase II core ... Kalkhoven E, Wissink S, van der Saag PT, van der Burg B (March 1996). "Negative interaction between the RelA(p65) subunit of NF ... RNA polymerase II transcription factor activity, sequence-specific DNA binding. • transcriptional activator activity, RNA ... transcription initiation from RNA polymerase II promoter. • signal transduction. • steroid hormone mediated signaling pathway. ...
... and the sigma factor subunits of RNA polymerase. Endospores of the bacterium Bacillus anthracis were used in the 2001 anthrax ... In endospore formation, the bacterium divides within its cell wall, and one side then engulfs the other.[3] Endospores enable ... "Archived from the original on March 3, 2016. Retrieved June 18, 2016.. ... This chemical accounts for up to 10% of the spore's dry weight.[3] ...
negative regulation of transcription from RNA polymerase II promoter. • proteolysis. • regulation of synaptic plasticity. • ... Annaert WG, Esselens C, Baert V, Boeve C, Snellings G, Cupers P, Craessaerts K, De Strooper B (November 2001). "Interaction ... Tesco G, Kim TW, Diehlmann A, Beyreuther K, Tanzi RE (December 1998). "Abrogation of the presenilin 1/beta-catenin interaction ... Stahl B, Diehlmann A, Südhof TC (April 1999). "Direct interaction of Alzheimer's disease-related presenilin 1 with armadillo ...
DNA polymerase binding. • protein C-terminus binding. • protein binding. • four-way junction DNA binding. • identical protein ... Three other microRNAs were identified, by various criteria, as likely to repress RAD51 (miR-96,[48] miR-203,[49] and miR-103/ ... "Stable interaction between the products of the BRCA1 and BRCA2 tumor suppressor genes in mitotic and meiotic cells". Molecular ... messenger RNA. [13]. Breast cancer (progesteron receptor negative). Over-expression. -. messenger RNA. [16]. ...
The mRNA encodes RNA dependent RNA polymerase. This polymerase makes complementary minus strands of RNA, then uses them as ... Step 2: The 3C dimer opens the RNA stem by forming a more stable interaction with single strands forming the stem. Step 3: ... Complete genome analysis of three novel picornaviruses from diverse bat species. J Virol. Carter, J., & Saunders, V. A. (2007 ... the RNA polymerase). Genomic RNAs of picornaviruses possess multiple RNA elements and they are required for both negative and ...
Werner F (September 2007). "Structure and function of archaeal RNA polymerases". Mol. Microbiol. 65 (6): 1395-404. doi:10.1111/ ... a b Kandler O. The early diversification of life and the origin of the three domains: A proposal. In: Wiegel J, Adams WW, ... The well-characterized interactions between archaea and other organisms are either mutual or commensal.[184] There are no clear ... Transcription in archaea more closely resembles eukaryotic than bacterial transcription, with the archaeal RNA polymerase being ...
POLR3K: encoding enzyme DNA-directed RNA polymerase III subunit RPC10. *PRR35: encoding protein Proline rich 35 ... G-banding patterns of human chromosome 16 in three different resolutions (400,[14] 550[15] and 850[4]). Band length in this ... SNORD71: encoding protein Small nucleolar RNA, C/D box 71. *SPSB3: encoding protein SplA/ryanodine receptor domain and SOCS box ... LINC00273 encoding protein Long intergenic non-protein coding RNA 273. *LOC124220: encoding protein Zymogen granule protein 16 ...
"RNA polymerase III accurately initiates transcription from RNA polymerase II promoters in vitro". The Journal of Biological ... TFIIA-DNA complex through interactions both upstream and downstream of the TATA box.[24] RNA polymerase II is then recruited to ... a cofactor for RNA polymerase II, or by TFIID.[11] When promoters use the SAGA/TATA box complex to recruit RNA polymerase II, ... although some in vitro studies have demonstrated that RNA polymerase III can recognize TATA sequences.[25] ...
Li J, Moazed D, Gygi SP (Dec 2002). "Association of the histone methyltransferase Set2 with RNA polymerase II plays a role in ... Three histone modifications are particularly associated with repressed genes: Trimethylation of H3 lysine 27 (H3K27me3). This ... In all, histones make five types of interactions with DNA: *Helix-dipoles form alpha-helixes in H2B, H3, and H4 cause a net ... RNA polymerase II undergoes a switch from initiating' to 'elongating', marked by a change in the phosphorylation states of the ...
Interactions between RNA and metal ions can be probed by a number of methods, including observing changes in chemical shift ... Nucleotides uniformly enriched in 13C and/or 15N can be obtained through biochemical methods, by performing polymerase chain ... such as DNA or RNA. It is useful for molecules of up to 100 nucleotides, and as of 2003, nearly half of all known RNA ... For RNA, the differences in chemical structure and helix geometry make this assignment more technically difficult, but still ...
"The bromodomain protein Brd4 is a positive regulatory component of P-TEFb and stimulates RNA polymerase II-dependent ... Wimmer J, Fujinaga K, Taube R, Cujec TP, Zhu Y, Peng J, Price DH, Peterlin BM (1999). "Interactions between Tat and TAR and ... Michels AA, Nguyen VT, Fraldi A, Labas V, Edwards M, Bonnet F, Lania L, Bensaude O (2003). "MAQ1 and 7SK RNA interact with CDK9 ... Nguyen VT, Kiss T, Michels AA, Bensaude O (2001). "7SK small nuclear RNA binds to and inhibits the activity of CDK9/cyclin T ...
A fundamental building block of RNA structure crucial to RNA function in diverse biological systems". EMBO Rep. 1 (1): 18-23. ... There are three amino acids encoded by six different codons: serine, leucine, and arginine. Only two amino acids are specified ... Degeneracy of codons is the redundancy of the genetic code, exhibited as the multiplicity of three-base pair codon combinations ... There is only one threefold degenerate site where changing to three of the four nucleotides may have no effect on the amino ...
Interactions[edit]. HADHB is a functional molecular target of ERα in the mitochondria, and the interaction may play an ... RNA binding. • acetyl-CoA C-acyltransferase activity. • long-chain-enoyl-CoA hydratase activity. ... "Vectorial proteomics reveal targeting, phosphorylation and specific fragmentation of polymerase I and transcript release ... The encoded protein can also bind RNA and decreases the stability of some mRNAs. The genes of the alpha and beta subunits of ...
positive regulation of transcription from RNA polymerase II promoter. • negative regulation of cell proliferation. • signal ... Yao R, Maeda T, Takada S, Noda T (2001). "Identification of a PDZ domain containing Golgi protein, GOPC, as an interaction ...
... that has three segments (S, M, and L segments). The L segment is 9010 nt and encodes for the RNA-dependent RNA polymerase (RdRp ... Missing or empty ,title= (help) Whitfield, Anna; Ullman, Diane; German, Thomas (2005). "Tospovirus-Thrips Interactions". Annual ... Like other members of Bunyavirales, this virus is enveloped and has a negative sense single-stranded RNA (−ssRNA) genome ... The N protein contributes to viral replication, and coats the genomic RNA within the virion. Presently the soybean thrips ( ...
RNA) - ribosome - RNA - route of administration - RT-PCR - RTI - Ryan White C.A.R.E. act ... polymerase - Polymerase chain reaction (PCR) - polyneuritis - polypeptide - polyvalent vaccine - post-exposure prophylaxis (PEP ... phase III trials - phase IV trials - photosensitivity - PHS - pituitary gland - placebo - placebo controlled study - placebo ... interaction - interferon - interleukin-1 (IL-1) - interleukin-2 (IL-2) - interleukin-4 (IL-4) - interleukin-12 (IL-12) - ...
RNA polymerase II transcription coactivator activity. • transcription factor binding. • activating transcription factor binding ... Interaction with translocation protein B23.». J. Biol. Chem. 269 (38): 23776-83. PMID 8089149. !CS1 manut: Uso explícito de et ... regulation of transcription from RNA polymerase II promoter. • cellular response to UV. • ribosomal large subunit export from ... positive regulation of transcription from RNA polymerase II promoter. • negative regulation of mRNA splicing, via spliceosome. ...
Research on psoralen has historically focused on interactions with DNA and RNA (in particular, ICL formation). Psoralen, ... One inaccurate process for repairing psoralen crosslinks appears to employ a DNA polymerase to fill in the gap formed in the ... and therefore have been used extensively for the analysis of interactions and structures for both DNA and RNA.[26][27] ... Several physicochemical methods have been employed to derive binding constants for psoralen-DNA interactions. Classically, two ...
... an RNA-dependent DNA polymerase, to produce DNA from the initial virion RNA genome. This DNA is often integrated into the host ... There are two strands of RNA within the cell that have three enzymes: protease, reverse transcriptase, and integrase (1). The ... 2010). Lentiviruses and Macrophages: Molecular and Cellular Interactions. Caister Academic. ISBN 978-1-904455-60-8. .. ... RNA: consists of a dimer RNA. It has a cap at the 5' end and a poly(A) tail at the 3' end. The RNA genome also has terminal ...
Werner F (2007). "Structure and function of archaeal RNA polymerases". Mol. Microbiol. 65 (6): 1395-404. PMID 17697097. doi: ... O flaxelo bacteriano ten homoloxías co sistema de secreción de tipo III,[101][102] mentres que o flaxelo arqueano parece que ... and surface layer-cell wall interactions". J Struct Biol 124 (2-3): 276-302. PMID 10049812. doi:10.1006/jsbi.1998.4070.. ... 45,0 45,1 Kandler O. The early diversification of life and the origin of the three domains: A proposal. In: Wiegel J, Adams WW ...
transcription initiation from RNA polymerase II promoter. • G1/S transition of mitotic cell cycle. • negative regulation of ... Dai K, Kobayashi R, Beach D (1996). "Physical interaction of mammalian CDC37 with CDK4". J. Biol. Chem. 271 (36): 22030-4. doi: ... Dai K, Kobayashi R, Beach D (1996). "Physical interaction of mammalian CDC37 with CDK4". J. Biol. Chem. 271 (36): 22030-4. doi: ... Lamphere L, Fiore F, Xu X, Brizuela L, Keezer S, Sardet C, Draetta GF, Gyuris J (1997). "Interaction between Cdc37 and Cdk4 in ...
negative regulation of transcription from RNA polymerase II promoter. • positive regulation of NF-kappaB transcription factor ... Following TRADD binding, three pathways can be initiated.[30][31] *Activation of NF-κB: TRADD recruits TRAF2 and RIP. TRAF2 in ... Interactions[edit]. TNFα has been shown to interact with TNFRSF1A.[45][46] ... positive regulation of transcription from RNA polymerase II promoter. • positive regulation of leukocyte adhesion to arterial ...
... involved in RNA capping. RNA2 (2.9 kb) encodes the 2a protein (94 kDa), the RNA-dependent RNA polymerase, responsible for ... BMV has a genome that is divided into three 5' capped RNAs. RNA1 (3.2 kb) encodes a protein called 1a (109 kDa), which contains ... Portion of thesis entitled Characterization of Brome Mosaic Virus RNA3 interaction with GCD10, a tRNA binding host factor from ... The sequence similarities of RNA replication genes and strategies for BMV have been shown to extend to a wide range of plant ...
In TMV, this extra sequence of polypeptide is an RNA polymerase that replicates its genome. Some viruses use the production of ... 17% of plant viruses are ssDNA and very few are dsDNA, in contrast a quarter of animal viruses are dsDNA and three quarters of ... but those studies that exist almost overwhelmingly show that such interactions between wild plants and their viruses do not ... Instead, the naked viral RNA may alter the function of the cells through a mechanism similar to RNA interference, in which the ...
Polymerase chain reaction[edit]. Polymerase chain reaction (PCR) assays are the most commonly used molecular technique to ... Once the virus has gained access to the host's cells, the virus' genetic material (RNA or DNA) must be introduced to the cell. ... Most DNA viruses assemble in the nucleus while most RNA viruses develop solely in cytoplasm.[22][23] ... The 3 main types of media used for testing are:[26] *Solid culture: A solid surface is created using a mixture of nutrients, ...
Because of the absence of RNA proofreading enzymes, the RNA-dependent RNA polymerase that copies the viral genome makes an ... Three of the four types of influenza viruses affect humans: Type A, Type B, and Type C.[2][6] Type D has not been known to ... an alternative approach has been proposed where the periodic pandemics are produced by interactions of a fixed set of viral ... The viral RNA (vRNA) molecules, accessory proteins and RNA-dependent RNA polymerase are then released into the cytoplasm (Stage ...
negative regulation of transcription from RNA polymerase II promoter. • osteoblast differentiation. • in utero embryonic ... Interactions[edit]. Insulin-like growth factor 2 has been shown to interact with IGFBP3[15][16][17][18] and transferrin.[15] ... Insulin-like growth factor 2 (IGF-2) is one of three protein hormones that share structural similarity to insulin. The MeSH ... positive regulation of transcription from RNA polymerase II promoter. • positive regulation of insulin receptor signaling ...
Interactions between three common subunits of yeast RNA polymerases I and III. D Lalo, C Carles, A Sentenac, and P Thuriaux ... Interactions between three common subunits of yeast RNA polymerases I and III ... Interactions between three common subunits of yeast RNA polymerases I and III ... Interactions between three common subunits of yeast RNA polymerases I and III ...
Hepatitis C virus (HCV) NS5B protein is the viral RNA-dependent RNA polymerase capable of directing RNA synthesis. In this ... Interestingly, the conserved motifs of RNA-dependent RNA polymerase for putative RNA binding (220-DxxxxD-225) and template/ ... Specific Interaction between the Hepatitis C Virus NS5B RNA Polymerase and the 3′ End of the Viral RNA. Ju-Chien Cheng, Ming-Fu ... coding region of HCV genomic RNA important for the binding of NS5B RNA polymerase. In addition, two RNA-binding domains of the ...
Common component of RNA polymerases I and III which synthesize ribosomal RNA precursors and small RNAs, such as 5S rRNA and ... DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as ... transcription by RNA polymerase III Source: PomBaseInferred from genetic interactioni*. "Isolation and characterization of the ... RNA Polymerase I Transcription Initiation. R-SPO-73772, RNA Polymerase I Promoter Escape. R-SPO-76061, RNA Polymerase III ...
Common component of RNA polymerases I, II and III which synthesize ribosomal RNA precursors, mRNA precursors and many ... functional non-coding RNAs, and small RNAs, such as 5S rRNA and tRNAs, respectively. ... DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as ... R-HSA-77075 RNA Pol II CTD phosphorylation and interaction with CE. R-HSA-8851708 Signaling by FGFR2 IIIa TM. R-HSA-9018519 ...
Interaction between a complex of RNA polymerase III subunits and the 70- kDa component of transcription factor IIIB. / Werner, ... Werner M, Chaussivert N, Willis IM, Sentenac A. Interaction between a complex of RNA polymerase III subunits and the 70- kDa ... Werner, M. ; Chaussivert, N. ; Willis, I. M. ; Sentenac, A. / Interaction between a complex of RNA polymerase III subunits and ... title = "Interaction between a complex of RNA polymerase III subunits and the 70- kDa component of transcription factor IIIB", ...
Retinoblastoma protein disrupts interactions required for RNA polymerase III transcription. Josephine E. Sutcliffe, T. R P ... Retinoblastoma protein disrupts interactions required for RNA polymerase III transcription. Molecular and Cellular Biology. ... Retinoblastoma protein disrupts interactions required for RNA polymerase III transcription. / Sutcliffe, Josephine E.; Brown, T ... title = "Retinoblastoma protein disrupts interactions required for RNA polymerase III transcription",. abstract = "The ...
... and an elongation complex containing a eukaryotic RNA polymerase. The structures and structural coordinates are useful in ... Crystals and structures are provided for an eukaryotic RNA polymerase, ... The atomic structure of RNA polymerase II in the act of transcription reveals the protein-DNA and -RNA interactions underlying ... In a particular embodiment the RNA polymerase of the crystal is a yeast RNA polymerase II. Such a RNA polymerase comprises 10 ...
Cryo-EM structure of yeast RNA polymerase III elongation complex at 3. 9 A ... 2D Diagram & Interactions. 3D Interactions. ZN. Query on ZN. Download SDF File Download CCD File A, B, I, J, L ... spliceosomal U6 small nuclear RNA and ribosomal 5S RNA is carried out by RNA polymerase III (Pol III), the largest yet ... spliceosomal U6 small nuclear RNA and ribosomal 5S RNA is carried out by RNA polymerase III (Pol III), the largest yet ...
3D View: Structure , Electron Density , Ligand Interaction. Global Symmetry: Asymmetric - C1 Global Stoichiometry: Monomer - A1 ... Three-dimensional structure of a RNA-polymerase II binding protein with associated ligand.. *DOI: 10.2210/pdb1TA0/pdb ... Recycling of RNA polymerase II (Pol II) after transcription requires dephosphorylation of the polymerase C-terminal domain (CTD ... Recycling of RNA polymerase II (Pol II) after transcription requires dephosphorylation of the polymerase C-terminal domain (CTD ...
Interactions less. * Polr3f interacts with 311 markers (Mir1a-1, Mir1a-2, Mir1b, ...) View All ... 3 phenotypes from 1 allele in 1 genetic background 8 phenotype references ...
Study of functional interactions between Histones Acetyl-transferases and RNA Polymerases I, II and III in the yeast ... The aim of our project is to study the role of the different HAT in the Pol I, II and III transcription systems. ...
R-HSA-76042 RNA Polymerase II Transcription Initiation And Promoter Clearance * R-HSA-76061 RNA Polymerase III Transcription ... R-HSA-73777 RNA Polymerase I Chain Elongation * R-HSA-73779 RNA Polymerase II Transcription Pre-Initiation And Promoter Opening ... Pathways & interactions: RNA polymerase, subunit H/Rpb5, conserved site (IPR020608). Pathways. Reactome * R-HSA-112382 ... R-HSA-76066 RNA Polymerase III Transcription Initiation From Type 2 Promoter ...
Molecular Interactions in the Initiation of rRNA Synthesis in Prokaryotes. The Regulation of RNA Polymerase by Guanosine 5- ... III. Highly Phosphorylated Nucleotides in Eukaryotes. 1. Isolation and Characterization of Novel Dinucleotides. Studies on the ... 2. Effect of ppGpp on RNA Polymerase and Transcription in E. Coli. ... Diadenosine Tetraphosphate (Ap4A)-A Ligand of DNA Polymerase α and Trigger of Replication. Elevated Nuclear ATP Pools and ATP/ ...
Interactions with other relevant participants such as small molecules (purple), sub-complexes (yellow), and other subunits (red ... RNA polymerase III type 2 promoter sequence-specific DNA binding. *polymerase III regulatory region sequence-specific DNA ... Complex: DNA-directed RNA polymerase III complex Macromolecular complex annotations are imported from the Complex Portal. These ... 2013) Yeast RNA polymerase III transcription factors and effectors. Biochim Biophys Acta 1829(3-4):283-95 PMID: 23063749 *SGD ...
A protein-protein interaction map of yeast RNA polymerase III.. Flores A, Briand JF, Gadal O, Andrau JC, Rubbi L, Van Mullem V ... The Arabidopsis TRM1-TON1 interaction reveals a recruitment network common to plant cortical microtubule arrays and eukaryotic ... The branching gene RAMOSUS1 mediates interactions among two novel signals and auxin in pea. ...
Additionally, the interaction between TFIIIB and RNA polymerase III is also impaired. Finally, the amount of tRNA is markedly ... D. Crighton, A. Woiwode, C. Zhang et al., "p53 represses RNA polymerase III transcription by targeting TBP and inhibiting ... "The p53 tumor suppressor protein represses human snRNA gene transcription by RNA polymerases II and III independently of ... 5. p53-Dependent Repression by Regulation of Noncoding RNAs. In recent years, nonprotein coding RNAs (ncRNA) have emerged as ...
We show that Lar7 has conserved RNA-recognition motifs, which bind telomerase RNA to protect it from exosomal degradation. In ... Here the authors identify Lar7 as a member of the full complex that helps to stabilise it and protect telomerase RNA from ... The telomerase holoenzyme is minimally composed of the reverse transcriptase and the RNA template. ... addition, Lar7 is required to stabilise the association of telomerase RNA with the protective complex LSm2-8, and telomerase ...
Eukaryotic nuclei contain three distinct types of RNA polymerases that differ in the RNA they synthesise: *RNA polymerase I: ... Functional interaction of the subunit 3 of RNA polymerase II (RPB3) with transcription factor-4 (ATF4).. FEBS Lett. 547 15-9 ... RNA polymerase II: occurs in the nucleoplasm, synthesises mRNA precursors. *RNA polymerase III: also occurs in the nucleoplasm ... there are three different forms of DNA-directed RNA polymerases transcribing different sets of genes. Most RNA polymerases are ...
The Regulation of RNA Polymerase II Activity by Multisite Phosphorylation.- Transcription Factors of RNA Polymerase III from ... Analysis of Subunit Interactions in Steroid Receptors using Chemical Cross-Linking.- Possible Functional Interaction between ...
Interaction between a complex of RNA polymerase III subunits and the 70-kDa component of transcription factor IIIB. J. Biol. ... The RNA cleavage activity of RNA polymerase III is mediated by an essential TFIIS-like subunit and is important for ... RNA polymerase III (Pol III) produces essential components of the biosynthetic machinery, and therefore its activity is tightly ... TOR signaling controls all three RNA polymerase systems involved in ribosome biogenesis (22, 39, 42). Polymerase II (Pol II)- ...
Characterization of the interaction between RNA Polymerase III and Ty1-Integrase. *The role of the Nuclear Pore Complex in ... 2016) "Ty1 integrase interacts with RNA Polymerase III-specific subcomplexes to promote insertion of Ty1 elements upstream of ... Retrotransposons are repetitive DNA elements in the genome that can replicate and insert a new copy into the genome via an RNA ... 2012) "Interactions between the kinetochore complex and the protein kinase A pathway in Saccharomyces cerevisiae" G3 (Bethesda ...
Mapping the protein interaction network for TFIIB-related factor Brf1 in the RNA polymerase III preinitiation complex. ... The TFIIE-related Rpc82 subunit of RNA polymerase III interacts with the TFIIB-related transcription factor Brf1 and the ... RNA sequencing of archived neonatal dried blood spots.. Bybjerg-Grauholm J, Hagen CM, Khoo SK, Johannesen ML, Hansen CS, Bækvad ... Respiratory viruses in young South African children with acute lower respiratory infections and interactions with HIV. ...
MCB130048P David Cowburn, Yeshiva University; Dynamic Interaction of Metabolic Regulator Maf1 and Pol III RNA Polymerase [ ... MCB100019P Wonmuk Hwang, Texas A&M University; Role of Nonspecific Interactions for the Motion of the Kinesin Motor Near the ... MCB160080P Michael Feig, Michigan State University; Long-Time Kinetics of Protein-Protein Interactions in Conjunction with Trp- ... MCB110023P Matthias Buck, Case Western Reserve University; Raf Domain Interactions and the Role of 14-3-3 Association [ ...
RNA polymerase II complex import to nucleus. IBA. --. GO:1990022. RNA polymerase III complex localization to nucleus. IBA. --. ... STRING Interaction Network Preview (showing 5 interactants - click image to see 25) Selected Interacting proteins: ... Human GTPases associate with RNA polymerase II to mediate its nuclear import. (PMID: 21768307) Carré C … Shiekhattar R ( ... Small GTPase required for proper nuclear import of RNA polymerase II (RNAPII) (PubMed:20855544, PubMed:21768307). May act at an ...
Small-molecule inhibition of MLL activity by disruption of its interaction with WDR5 Biochem J (December, 2012) ... RNA synthesis in the eukaryote nucleus is carried out by the multisubunit RNA polymerases (Pols) I, II and III. Whereas Pol I ... Pol III transcribes small RNAs, including transfer RNAs, 5 S ribosomal RNA and U6 small nuclear RNA. Structural studies have so ... Structure of the complete RNA polymerase II-RNA inhibitor complex [23]. The polymerase is shown as a silver ribbon model and ...
... the polymerase responsible for synthesizing transfer and small ribosomal RNAs in eukaryotes. The carboxy-terminal domain of ... this subunit shares a high degree of sequence similarity to the carboxy-terminal domain of an RNA polymerase II elongation ... This gene encodes a small essential subunit of RNA polymerase III, ... POLR3K has direct interactions with proteins and molecules. Those interactions were detected by several methods such as yeast ...
... to evaluate whether sites of TRE5-A integration coincide with RNA polymerase III complexes. To analyze the influence of local ... We follow the working hypothesis that protein interactions between retrotransposon-derived proteins and tRNA gene-specific ... TRE5-A retrotransposition profiling reveals putative RNA polymerase III transcription complex binding sites on the ... CASE Industrial Placement:The three month-CASE placement will be at Syngentas International Research Stations in the UK or ...
RNA polymerase 2 subunit RPB3 (53), ZIP kinase (54), and HTLV1 transactivator Tax (55). This specific interaction is required ... The screening yielded three positive clones encoding different truncated forms of ATF4. The interaction between ATF4 and IRF7 ... RNA isolation and RT-PCR. Total RNA was isolated from cells with the RNeasy Plus Mini Kit (Qiagen). First-strand cDNA was ... Functional interaction of the subunit 3 of RNA polymerase II (RPB3) with transcription factor-4 (ATF4). FEBS Lett. 547: 15-19. ...
A protein-protein interaction map of yeast RNA polymerase III. Proc. Natl. Aced. Sci. USA 96:7815-7820. ... RNA polymerase III defects suppress a conditional-lethal poly(A) polymerase mutation in Saccharomyces cerevisiae. Genetics 143: ... Transcription factor IIIB generates extended DNA interactions in RNA polymerase III transcription complexes on tRNA genes. Mol ... Novel Small-Molecule Inhibitors of RNA Polymerase III. Liping Wu, Jing Pan, Vala Thoroddsen, Deborah R. Wysong, Ronald K. ...
Interactions of yeast RNA polymerase III and its transcription factors with the promoter ... An interaction network linking the RNA polymerase II general transcription machinery to factors involved in RNA processing, ... PITSLRE p110 protein kinases associate with transcription elongation factors, RNA polymerase II and an RNA polymerase II ... Detection of interactions of basal transcription factors with specific structural features of RNA polymerases ...
  • Crystals and structures are provided for an eukaryotic RNA polymerase, and an elongation complex containing a eukaryotic RNA polymerase. (freepatentsonline.com)
  • The structures and structural coordinates are useful in structural homology deduction, in developing and screening agents that affect the activity of eukaryotic RNA polymerase, and in designing modified forms of eukaryotic RNA polymerase. (freepatentsonline.com)
  • The Arabidopsis TRM1-TON1 interaction reveals a recruitment network common to plant cortical microtubule arrays and eukaryotic centrosomes. (nih.gov)
  • Eukaryotic cells are also known to contain separate mitochondrial and chloroplast RNA polymerases. (ebi.ac.uk)
  • Eukaryotic RNA polymerases, whose molecular masses vary in size from 500 to 700 kDa, contain two non-identical large (>100 kDa) subunits and an array of up to 12 different small (less than 50 kDa) subunits. (ebi.ac.uk)
  • It has been shown [ PMID: 12694606 , PMID: 2187864 , PMID: 8367291 , PMID: 12860379 ] that small subunits of about 30 to 40 kDa found in archebacterial and all three types of eukaryotic polymerases are highly conserved. (ebi.ac.uk)
  • For example, a central step in initiation by eukaryotic RNA polymerase II (Pol II) on many protein-coding genes is binding of transcription factor (TF) IID, via its TATA-binding protein (TBP) subunit, to an upstream TATA box promoter element. (asm.org)
  • Therefore, we speculated that the viral polymerase (Pol), while bound to the 5′ ε stem-loop structure, could recognize the cap via its interaction with eIF4E, a eukaryotic translation initiation factor. (asm.org)
  • The eukaryotic core RNA polymerase II was first purified using transcription assays. (wikiversity.org)
  • Noncoding RNAs (ncRNAs) are emerging as key regulators of eukaryotic mRNA transcription, often functioning as transacting factors akin to prototypical protein transcriptional regulators. (aacrjournals.org)
  • Structure and functions of eukaryotic DNA-dependent RNA polymerase I. (springer.com)
  • Invasion is facilitated by a type III secretion system that allows the bacteria to secrete effector proteins into the eukaryotic host cell. (asm.org)
  • Eukaryotic organisms contain all three of these amines at abundant levels (high micromolar to millimolar). (embopress.org)
  • Matters are more complicated in Archaea, which contain an unusual D-family DNA polymerase (PolD) in addition to PolB, a B-family replicative DNA polymerase that is homologous to the eukaryotic ones. (portlandpress.com)
  • The eukaryotic mRNA 3′-end processing machinery interacts with the transcription machinery and adds the polyadenylation tail on the mRNA substrate. (sciencemag.org)
  • Newly transcribed eukaryotic precursor messenger RNAs (pre-mRNAs) are processed at their 3′ ends by the ~1-megadalton multiprotein cleavage and polyadenylation factor (CPF). (sciencemag.org)
  • Messenger RNA (mRNA) 3′ end formation is a nuclear process through which all eukaryotic primary transcripts are endonucleolytically cleaved and most of them acquire a poly(A) tail. (pubmedcentralcanada.ca)
  • Here, we review the eukaryotic 3′ end processing machineries as well as the comprehensive set of regulatory factors and discuss the different molecular mechanisms of 3′ end processing regulation by proposing several overlapping models of regulation. (pubmedcentralcanada.ca)
  • RNA polymerase III (Pol III) 2 is the most complex of the three eukaryotic RNA polymerases and is responsible for the synthesis of various small, noncoding RNAs including tRNAs ( 1 ). (pubmedcentralcanada.ca)
  • The replication appears to take place at the perinuclear endoplasmic reticulum and requires virus-encoded proteins NS3 (proteinase/helicase) and NS5 (polymerase) as components of the presumed replicative complex ( 36 ). (asm.org)
  • It is generally believed that conserved sequences and structures at the 3′ terminus of viral genomic RNA function as cis -acting signals that interact with viral and cellular proteins to initiate the synthesis of minus-strand RNA during viral replication. (asm.org)
  • A system that detects the formation of complexes between different proteins by linking them to separate domains of the GAL4 transcription activator protein has been used to study protein-protein interactions between four essential and unique subunits of yeast RNA polymerase III (C82, C53, C34 and C31), the 70-kDa component of the initiation transcription factor IIIB (TFIIIB 70 ) and the TATA-binding protein. (elsevier.com)
  • Whether the DNA-binding domain is always needed for direct DNA binding to target gene promoters or interaction of p53 with other proteins through this domain can evoke p53-dependent repression, remains to be elucidated. (hindawi.com)
  • The 3′ end of exon 1 has a binding site for Sm family proteins. (nature.com)
  • Sm and Sm-like proteins (LSm) are evolutionarily conserved families that function broadly in the processing of RNA. (nature.com)
  • POLR3K has direct interactions with proteins and molecules. (creativebiomart.net)
  • Forms an interface between the RNA polymerase II enzyme and chaperone/scaffolding proteins, suggesting that it is required to connect RNA polymerase II to regulators of protein complex formation (PubMed:17643375). (genecards.org)
  • Phosphatidylinositol 3-kinase-related protein kinase (PIKK) family proteins play essential roles in DNA-based and RNA-based processes, such as the response to DNA damage, messenger RNA (mRNA) quality control, transcription, and translation, where they contribute to the maintenance of genome integrity and accurate gene expression. (sciencemag.org)
  • The adenosine triphosphatases associated with diverse cellular activities (AAA+) family proteins RuvB-like 1 (RUVBL1) and RUVBL2 are involved in various cellular processes, including transcription, RNA modification, DNA repair, and telomere maintenance. (sciencemag.org)
  • The pgRNA serves not only as the RNA template for viral reverse transcription but also as the mRNA that is used to encode two viral proteins required for viral genome replication: the core (capsid, or C) protein and the polymerase (Pol, or reverse transcriptase). (asm.org)
  • LARP7 proteins are characterised by an N-terminal RNA-binding domain termed a La-motif and two RNA-recognition motifs (RRMs). (wikiversity.org)
  • LARP7 proteins associate with non-coding RNAs transcribed by RNA polymerase III, which have a hallmark 3′-UUU-OH tag 29 . (wikiversity.org)
  • Additionally, we identified structural similarity between Lar7 and the human protein hLARP7 (La-related protein 7 or La ribonucleoprotein domain family member 7), which is part of the wider LARP7 family of non-coding RNA-binding proteins 35 . (wikiversity.org)
  • Due to the vital biological importance of RNA and proteins functioning together within a cell, a protocol volume describing experimental procedures to study their interactions should find a home in many laboratories. (springer.com)
  • Since HCV does not carry any known oncogene, it is thought that interaction between virally encoded proteins and host proteins is responsible for carcinogenesis. (hindawi.com)
  • Many crucial interactions between HCV-encoded proteins and host proteins have been reported. (hindawi.com)
  • In this review we focus on the interaction of viral proteins with important regulators of cell cycle-oncoproteins YB-1, p53, and cyclin D1-which play a major role in cell proliferation, apoptosis, DNA repair, and genomic stability. (hindawi.com)
  • Genetic variants of HCV accumulate in patients and alter these interactions of host cell proteins. (hindawi.com)
  • Therefore, understanding the interactions between viral proteins and host cell proteins is very important to develop drugs for these liver diseases and HCC. (hindawi.com)
  • Compared to the T3 and T7 bacteriophage polymerases, POLRMT requires auxiliary proteins to recognize the promoter. (news-medical.net)
  • Using the yeast structure as a template, we have modeled the homologous domains from elongin A and CRSP70 and identified a conserved positively charged patch on the surface of all three proteins, which may be involved in conserved functional interactions with the transcriptional machinery. (embl-heidelberg.de)
  • To determine the reason for this species specificity, chimeras were constructed linking three structurally independent regions of the TFIIS proteins from yeast and human cells. (embl-heidelberg.de)
  • The 60S subunit contains 46 proteins and three RNA molecules: 25S RNA of 3392 nt, hydrogen bonded to the 5.8S RNA of 158 nt and associated with the 5S RNA of 121 nt. (wikipathways.org)
  • The 40S subunit has a single 18S RNA of 1798 nt and 33 proteins. (wikipathways.org)
  • In a rapidly growing yeast cell, 60% of total transcription is devoted to ribosomal RNA, and 50% of RNA polymerase II transcription and 90% of mRNA splicing are devoted to the production of mRNAs for r-proteins. (wikipathways.org)
  • Interaction mapping gives clear explanations about the organization of the proteome (organisms, tissues, and cells express the full complement of proteins) into functional units. (news-medical.net)
  • Y2H tools serve as access to the entire cellular proteome for screening protein-protein interaction, including membrane proteins, proteins localized in different subcellular compartments, and proteins that are transcriptionally active. (news-medical.net)
  • Protein-protein interaction that takes place between bait and prey proteins aids in activation of reporter genes that allow growth on a color reaction or specific media. (news-medical.net)
  • The variations in the Y2H system depend on the interaction of proteins. (news-medical.net)
  • The repressed transactivator (RTA) Y2H system was created to identify interactions that involve transcriptional activator proteins genetically. (news-medical.net)
  • Inhibition of interactions that involve proteins can be evaluated by screening growth in selective medium that contains 3-aminotriazole (3-AT). (news-medical.net)
  • The Polymerase III (Pol III) transcription system allows interaction of transcription factors with proteins by activating transcription that is based on RNA polymerase II. (news-medical.net)
  • Screening for interactions between extracellular proteins system (SCINEX-P system) permits the analysis of protein interactions that take place in the oxidizing environment of endoplasmic reticulum. (news-medical.net)
  • The interaction that occurs between the two hybrid proteins results in reconstitution of Ire1p dimerization and activation of unfolded protein response (UPR) downstream signaling. (news-medical.net)
  • In Zea mays , transcriptional regulation of the b1 ( booster1 ) gene requires a distal enhancer and MEDIATOR OF PARAMUTATION1 (MOP1), MOP2, and MOP3 proteins orthologous to Arabidopsis components of the RNA-dependent DNA methylation pathway. (genetics.org)
  • In all organisms, RNA synthesis is carried out by proteins - known as RNA polymerases (RNAPs) - that transcribe the genetic information from DNA in a highly-regulated, multi-stage process. (phys.org)
  • Using the Biomolecular Interaction Network Database (BIND - http://www.bind.ca ) [ 15 ] as an integration platform, we have collected 15,143 yeast protein-protein interactions among 4,825 proteins (about 75% of the yeast proteome). (springer.com)
  • Understanding the interactions between genes and proteins is essential for elucidating their function. (biomedcentral.com)
  • Subsequent resection of DSB 5′ strand ends generates 3′ single-stranded tails, which are substrates for proteins that search for a homologous DNA duplex and effect the templated repair of the break [3] . (prolekare.cz)
  • Several approaches are available for deducing the function of proteins in silico based upon sequence homology and physical or genetic interaction, yet this approach is limited to proteins with well-characterized domains, paralogs and/or orthologs in other species, as well as on the availability of suitable large-scale data sets. (mcponline.org)
  • Reciprocal analysis of Ydl156w protein interactions demonstrated a strong association with all four histones and also to proteins strongly associated with histones including Rim1, Rfa2 and 3, Yku70, and Yku80. (mcponline.org)
  • To gain deeper insights into the role of Ydl156w in histone biology we investigated the effect of the genetic deletion of ydl156w on H4 associated proteins, which lead to a dramatic decrease in the association of H4 with RNA polymerase III proteins. (mcponline.org)
  • That shift has given rise to a new field-proteomics-defined as the study of the structure, function and interactions of all proteins contained in a cell. (stowers.org)
  • We propose that the three GPN proteins execute a common, and likely essential, function in RNA polymerase assembly and transport. (labome.org)
  • While mutant PB2 proteins showed reduced nuclear accumulation, they formed polymerase complexes normally when co expressed with PB1 and PA. (nih.gov)
  • 12 ]. Protein interaction modules are composed of a variable number of proteins, with discrete functions arising from their individual constituents and their synergistic interactions. (biomedcentral.com)
  • Protein interaction networks are routinely represented as graphs, with proteins as nodes and interactions as edges. (biomedcentral.com)
  • In a graphical representation of a protein interaction network, a functional unit, or a group of functionally related proteins, is tightly connected as a community, while proteins from different functional units are more loosely connected. (biomedcentral.com)
  • RNA interference-mediated change in protein body morphology and seed opacity through loss of different zein proteins. (semanticscholar.org)
  • Both proteins, τ55-HPD and Huf, belong to the first branch of the histidine phosphatase family that has been originally termed the phosphoglycerate mutase family, according to its founding member, the eponymous enzyme, which catalyzes the conversion of 2-phosphoglycerate to 3-phosphoglycerate ( 6 , 7 ). (pubmedcentralcanada.ca)
  • Hepatitis C virus (HCV) NS5B protein is the viral RNA-dependent RNA polymerase capable of directing RNA synthesis. (asm.org)
  • In this study, an electrophoretic mobility shift assay demonstrated the interaction between a partially purified recombinant NS5B protein and a 3′ viral genomic RNA with or without the conserved 98-nucleotide tail. (asm.org)
  • Nevertheless, no direct interaction between the 3′ coding region and the HCV NS5A protein was detected. (asm.org)
  • The NS5B protein of HCV is a membrane-associated phosphoprotein ( 16 ) that possesses the conserved GDD motif of RNA-dependent RNA polymerase (RdRp) ( 19 ). (asm.org)
  • The retinoblastoma protein (RB) has been shown to suppress RNA polymerase (Pol) III transcription in vivo (R. J. White, D. Trouche, K. Martin, S. P. Jackson, and T. Kouzarides, Nature 382:88-90, 1996). (hud.ac.uk)
  • White, R. J. / Retinoblastoma protein disrupts interactions required for RNA polymerase III transcription . (hud.ac.uk)
  • 1TA0: Three-dimensional structure of a RNA-polymerase II binding protein with associated ligand. (rcsb.org)
  • Transactivation is achieved by interactions with components of the general transcription factor TFIID like the TATA box binding protein (TBP) [ 9 , 10 ] and TAFII31 [ 11 ]. (hindawi.com)
  • Conjugation stage-specific protein cnjC from Tetrahymena thermophila, which may be a stage-specific RNA polymerase subunit. (ebi.ac.uk)
  • The mode of coordination of the Pol II-dependent transcription of ribosomal protein genes with the activity of Pol I and Pol III is currently unknown. (asm.org)
  • Ma L., Ho K., Piggott N., Luo Z., Measday V . (2012) "Interactions between the kinetochore complex and the protein kinase A pathway in Saccharomyces cerevisiae" G3 (Bethesda). (ubc.ca)
  • Mouse B2 RNA binds Pol II and inhibits transcription of protein-coding genes during heat shock [ 17 , 18 ]. (portlandpress.com)
  • In contrast, the TATA box is dispensable for SNR6 transcription in vivo, apparently because TFIIIC bound to the intragenic A block and downstream B block can recruit TFIIIB via protein-protein interactions. (asm.org)
  • Initiation of transcription by an RNA polymerase at the start site of a gene involves multiple protein-DNA interactions, but one protein-DNA interaction often has a dominant role in specifying the site and efficiency of transcription complex assembly. (asm.org)
  • These findings suggest that Pol II and Pol III transcription initiation complexes may in fact have similar architectures but vary in the relative contributions of different protein-DNA and protein-protein interactions to the assembly pathway. (asm.org)
  • were successfully expressed from pZ7C-derived shuttle vectors, and their protein-protein binding interactions were analyzed in Z. mobilis ATCC 29191. (sigmaaldrich.com)
  • We show that a shuttle vector-based protein affinity 'pull-down' approach can be used to probe protein interaction networks in Z. mobilis cells. (sigmaaldrich.com)
  • Phosphatidylinositol 3-kinase (PI3K)-related protein kinases (PIKKs) are unconventional serine-threonine protein kinases, and their catalytic domains are homologous to the catalytic domains of phosphatidylinositol 3-kinases. (sciencemag.org)
  • We isolated the causal gene, which encodes a protein homologous to DNA-dependent RNA polymerase (RNAP) III subunit C4 (RPC4). (g3journal.org)
  • Based on this finding, we hypothesized that HBV Pol recognizes the cap structure via its interaction with eIF4E, a cap binding protein. (asm.org)
  • The F-box is a protein motif of approximately 50 amino acids that functions as a site of protein-protein interaction. (wikiversity.org)
  • Second, Elongin A, the transcriptionally active subunit of the Elongin (SIII) complex - which facilitates transcription elongation by RNA polymerase II [16] - is an F-box protein (Figure 2c). (wikiversity.org)
  • Here we redefine Pof8 as an RNA-binding protein of the ancient LARP7 family (Laribonucleoprotein domain family member 7 or La-related protein 7) and thus rename it Lar7. (wikiversity.org)
  • Further, we report a high degree of conservation between Lar7 and both the human protein hLARP7 and Tetrahymena thermophila telomerase RNA-binding protein p65. (wikiversity.org)
  • Lar7 is a member of the LARP7 RNA-binding protein family. (wikiversity.org)
  • RNA-Protein Interaction Protocols, Second Edition updates, complements, and expands upon the popular first edition by providing a collection of cutting-edge techniques developed or refined in the past few years along with tried-and-true methods. (springer.com)
  • The expert contributors explore the isolation and characterization of RNA-protein complexes, the analysis and measurement of RNA-protein interaction, and related novel techniques and strategies. (springer.com)
  • RNA polymerase II is a specific RNA polymerase that usually is the key part of the catalysis process that produces each RNA from the DNA gene or isoform to ultimately make a protein. (wikiversity.org)
  • The synthesis and processing of RNA require a complex network of protein-protein and protein-nucleic acids interactions. (rupress.org)
  • The encoded protein localizes to concentrated aggregates in the nucleus, and is required for transcription from all three types of polymerase III promoters. (wikipedia.org)
  • Biochemical assays to probe the network of protein-DNA interactions at the promoter revealed that B2 and Alu RNAs prevent Pol II from establishing contacts with the promoter both upstream and downstream of the TATA box. (aacrjournals.org)
  • LasR is the QS receptor protein which responds to the signal molecule N-(3-oxododecanoyl)homoserine lactone (3OC -HSL) and promotes signal production by increasing the transcription of the 3OC -HSL synthase gene, lasI. (bireme.br)
  • BRF2 is directly recruited to the TATA-box of polymerase III small nuclear RNA gene promoters through its interaction with the TATA-binding protein. (bio-medicine.org)
  • Here we identify an in vivo regulator of liquid-liquid phase separation through a genetic screen targeting factors required for Arabidopsis RNA-binding protein FCA function. (jic.ac.uk)
  • and (3) protein kinases that phosphorylate the CTD and a phosphatase that dephosphorylates it. (genetics.org)
  • For example, transcript elongation factor TFIIS is highly conserved among eukaryotes, and yet the TFIIS protein from Saccharomyces cerevisiae cannot function with mammalian RNA polymerase II and vice versa. (embl-heidelberg.de)
  • Yeast domain II alone is able to bind yeast RNA polymerase II with the same affinity as the full-length TFIIS protein, and this domain was expected to confer the species selectivity. (embl-heidelberg.de)
  • Ribosome biogenesis requires all three RNA polymerases: Pol I for rRNA genes, Pol II for ribosomal protein genes, and Pol III for tRNA and 5S RNA genes. (wikipathways.org)
  • Ribosomal protein genes and ribosomal biogenesis genes are transcribed by RNA polymerase II. (wikipathways.org)
  • B. Surface representation of the polymerase promoter-binding interface, showing the RNA-protein binding interface with the 3' vRNA extremity. (jle.com)
  • The first factor to interact with newly synthesized Pol III transcripts is the La protein. (rupress.org)
  • The yeast two-hybrid (Y2H) system is a genetic technique used to determine protein-protein interactions. (news-medical.net)
  • Y2H can be automated for high throughput researches of protein-protein interaction on a genome-wide scale. (news-medical.net)
  • These two classes form the basis of the protein-protein interaction detection system. (news-medical.net)
  • Modifications in this system enable it to be used for monitoring inhibitors of protein-protein interactions that involve libraries of small molecule compounds. (news-medical.net)
  • This method has also been utilized to identify inhibitions of four independent protein-protein interactions in screens that contained a 23,000 small molecule compound library. (news-medical.net)
  • The Hac1p unfolded protein response element that has been introduced into the reporter gene promoter helps in screening protein-protein interaction. (news-medical.net)
  • This Y2H system has been successfully implemented for analysis of interaction between Calnexin and protein disulfide isomerase ERp57, and interactions between antibodies and antigens. (news-medical.net)
  • Specifically we apply these tools to mechanistically dissect how the p53 tumor suppressor protein communicates with multiple transcription assemblies, such as chromatin remodeling factors (PBAF), core promoter recognition factors (TFIID), and RNA Polymerase II, to circumnavigate the repressive effects of chromatin on transcription. (yu.edu)
  • By shooting lasers at an RNA polymerase (RNAP) and a strand of DNA, scientists have learned a critical component of how a complex protein develops. (phys.org)
  • This paper describes a novel graph theoretic clustering algorithm, "Molecular Complex Detection" (MCODE), that detects densely connected regions in large protein-protein interaction networks that may represent molecular complexes. (springer.com)
  • Protein interaction and complex information from the yeast Saccharomyces cerevisiae was used for evaluation. (springer.com)
  • Dense regions of protein interaction networks can be found, based solely on connectivity data, many of which correspond to known protein complexes. (springer.com)
  • Here we present the first report that uses a clustering algorithm to identify molecular complexes in a large protein interaction network derived from heterogeneous experimental sources. (springer.com)
  • Predicting molecular complexes from protein interaction data is important because it provides another level of functional annotation above other guilt-by-association methods. (springer.com)
  • 20 ] focused on features based on protein-protein interaction (PPI) networks, such as node degree, centrality, and clustering coefficient. (biomedcentral.com)
  • Many years of research have established specific interactions between corresponding protein complexes and histones (reviewed in ( 6 , 7 ). (mcponline.org)
  • Washburn is a leader in applying mass spectrometry techniques to identify and quantify protein interactions, so much so that in July of 2011 he was named a "Rising Star in Molecular Biology and Genetics" by Thomson Reuters Science Watch. (stowers.org)
  • Comprehensive and up-to-date, RNA-Protein Interaction Protocols, Second Edition is an ideal guide for researchers continuing the study of this all-important biological partnership. (springer.com)
  • McQuibban G, Gong J, Tam E, McCulloch C, Clark Lewis I, Overall C. Inflammation dampened by gelatinase A cleavage of monocyte chemoattractant protein-3. (labome.org)
  • The conformational change is highlighted by isolated movement of three loops within the C-terminal domain, a domain movement unique to known class III binding protein structures. (labome.org)
  • Protein-coding genes in eukaryotes are transcribed by RNA polymerase II (Pol II) as precursor messenger RNAs (mRNAs), which undergo 5′ capping, splicing, and 3′-end processing. (sciencemag.org)
  • It is reported to contain 15 different subunits, including the Ysh1/CPSF73 endonuclease that cleaves the pre-mRNA, the Pap1/PAP polymerase that adds the polyadenylate [poly(A)] tail, and two protein phosphatases (Ssu72/SSU72 and Glc7/PP1) that regulate transcription and 3′-end processing ( 1 - 7 ). (sciencemag.org)
  • Human alpha importin isoforms associated efficiently to PB2 protein of an H3N2 human virus but bound to diminished and variable extents to PB2 from H5N1 avian or human strains, suggesting that the function of alpha importin during RNA replication is important for the adaptation of avian viruses to the human host. (nih.gov)
  • When glucose is low and lactose high, though, the cell makes cAMP, a starvation indicator, which binds to a protein called CAP (catabolite activator protein), which binds to a DNA binding site upstream of where the RNA polymerase usually binds in front of the lac operon. (evolutionnews.org)
  • We propose a new network decomposition method to systematically identify protein interaction modules in the protein interaction network. (biomedcentral.com)
  • In this paper, we address the issue of constructing protein interaction modules from the protein interaction data. (biomedcentral.com)
  • The concept of protein interaction modules as fundamental functional units was first outlined by Hartwell et al . (biomedcentral.com)
  • To detect protein interaction modules from protein interaction data, we use a graph theory approach. (biomedcentral.com)
  • Interacts (via CARD domain) with Human respiratory syncytial virus A non-structural protein 2 (NS2) and this interaction disrupts its interaction with MAVS, impeding downstream IRF3 activation. (phosphosite.org)
  • Cohesin is a large protein ring that topologically encircles DNA and participates in several chromosome functions, including sister chromatid cohesion, chromosome segregation, DNA repair, and gene expression (reviewed in [1] - [3] ). (prolekare.cz)
  • Protein phosphatase 6 regulatory subunit 3 (PPP6R3) is a regulatory subunit of the PP6 holoenzyme complex involved in the turnover of serine and threonine phosphorylation events during mitosis. (atlasgeneticsoncology.org)
  • PPP6R3, alias protein phosphatase 6 regulatory subunit 3, is a protein coding gene that starts at 68,460,718 nt and ends at 68,615,334 nt from qter (RefSeq NC_000011.10) and with a length of 154617 bp. (atlasgeneticsoncology.org)
  • Here, electrostatic properties of 17 T7 RNA-polymerase specific promoters where studied, , and this has been recently reported to play an important role in protein-nucleic acid recognition (1, 2). (jbsdonline.com)
  • A large number of protein factors associates with this machinery to regulate the efficiency and specificity of this process and to mediate its interaction with other nuclear events. (pubmedcentralcanada.ca)
  • The S. cerevisiae τ55 protein consists of an N-terminal domain predicted to belong to the histidine phosphatase family and a C-terminal domain necessary for its interaction with τ95. (pubmedcentralcanada.ca)
  • The N-terminal histidine phosphatase domain of the τ55 protein (τ55-HPD) is only found in hemiascomycetes, whereas corresponding subunits in other eukaryotes only contain the C-terminal moiety required for the interaction with τ95 orthologues ( 5 ). (pubmedcentralcanada.ca)
  • The conserved 5′ noncoding region (5′NCR) of the HCV genome ( 4 , 13 ) is highly structured and contributes to the internal ribosome entry site important for the translation initiation of HCV RNA ( 14 , 15 , 25 , 34 , 35 , 39 ). (asm.org)
  • It is reasonable to propose that following HCV infection, the initiation of minus-strand RNA synthesis depends on an initial recognition and specific binding of the NS5B RNA polymerase or replicative complex to the 3′ terminus of the viral genomic RNA. (asm.org)
  • Transcription initiation from promoter elements requires a sixth, dissociable subunit called a sigma factor, which reversibly associates with the core RNA polymerase complex to form a holoenzyme [ PMID: 3052291 ]. (ebi.ac.uk)
  • The TFIIE-related Rpc82 subunit of RNA polymerase III interacts with the TFIIB-related transcription factor Brf1 and the polymerase cleft for transcription initiation. (nih.gov)
  • First, the crystal structure of RNA polymerase (Pol) II in complex with an RNA inhibitor revealed that this RNA blocks transcription initiation by preventing DNA loading into the active-centre cleft. (portlandpress.com)
  • Comparison of the structure with Pol II elongation complex structures [ 11 , 16 ] reveals substantial overlap of the RNA inhibitor with nucleic acids in an elongation complex, and explains why FC RNA inhibits initiation, but not elongation [ 22 ]. (portlandpress.com)
  • Like FC RNA, B2 RNA inhibits initiation, but not elongation [ 18 ], although these two RNAs do not share sequence homology. (portlandpress.com)
  • The Saccharomyces cerevisiae U6 RNA gene, SNR6 , possesses upstream sequences that allow productive binding in vitro of the RNA polymerase III (Pol III) transcription initiation factor IIIB (TFIIIB) in the absence of TFIIIC or other assembly factors. (asm.org)
  • A key step in initiation by Pol III on tRNA genes is binding of TFIIIC to two intragenic promoter elements, the A and B blocks. (asm.org)
  • These distinct promoter recognition steps in Pol II and Pol III transcription imply divergent mechanisms for initiation complex assembly. (asm.org)
  • Yet further characterization of Pol II and Pol III transcription initiation complexes has revealed striking similarities. (asm.org)
  • Taking these findings together, the binding of Pol to ε thus triggers three critical steps in hepadnaviral replication, (i) the initiation of reverse transcription ( 23 ), (ii) translation suppression ( 19 ), and (iii) nucleocapsid assembly ( 11 , 15 ), leading to the selective incorporation of both Pol and pgRNA into the viral nucleocapsids. (asm.org)
  • TFIIIB150 is a subunit of the TFIIIB transcription initiation complex, which recruits RNA polymerase III to target promoters in order to initiate transcription. (wikipedia.org)
  • Within the 5′ NCR, an internal ribosome entry site is present to facilitate CAP-independent translation initiation of genomic RNA [ 5 ]. (hindawi.com)
  • Mitochondrial transcription is a three-step process as with genomic DNA transcription: initiation, elongation, and termination. (news-medical.net)
  • Background Information BRF2 is one of the multiple subunits of the RNA polymerase III transcription factor complex required for transcription of genes with promoter elements upstream of the initiation site. (bio-medicine.org)
  • Structural rearrangements of the T box riboswitch RNA in response to binding of the cognate uncharged tRNA regulate gene expression at the level of either transcription attenuation ( 2 , 3 ) or translation initiation ( 4 ). (pnas.org)
  • Formation of transcription initiation complexes at the rDNA promoter depends on the association of RNA Pol I with the Rrn3p transcription factor, which is regulated by phosphorylation/dephosphorylation of Rrn3p. (wikipathways.org)
  • In cells treated with rapamycin, Rrn3p is subject to proteasome-dependent degradation, reducing the cellular amount of transcription-initiation competent RNA Pol I - Rrn3p complexes. (wikipathways.org)
  • As TFIID is a central player in regulating transcription initiation by RNA Polymerase II, we want to understand how many different regulatory factors access this key core promoter recognition factor during transcription pre-initiation complex (PIC) formation. (yu.edu)
  • Their simulation of the initiation phase of transcription in bacterial RNA polymerase showed a three-step process. (phys.org)
  • A whole RNA silencing process comprises three stages: initiation, maintenance, and signal amplification. (frontiersin.org)
  • All the promoters have been erlier characterized in detail by their interaction with RNA-polymerase and transcription initiation (3, 4). (jbsdonline.com)
  • Recruitment of TFIIIB to most class III genes requires its binding to TFIIIC2, but this can be blocked by RB. (hud.ac.uk)
  • We classify two different categories of repressed genes based on the underlying mechanism, and novel mechanisms which involve regulation through noncoding RNAs are discussed. (hindawi.com)
  • In principle, two categories of p53-repressed target genes can be classified: (I) genes that are regulated by direct interaction of p53 with target gene promoters or bound cofactors, (II) genes that are indirectly regulated by other p53 target genes. (hindawi.com)
  • In eukaryotes, there are three different forms of DNA-directed RNA polymerases transcribing different sets of genes. (ebi.ac.uk)
  • In Escherichia coli , 6 S RNA binds RNA polymerase and inhibits transcription of housekeeping genes upon entry of stationary cell growth [ 19 - 21 ]. (portlandpress.com)
  • We have shown that during the heat shock response in mouse and human cells, small non-coding RNA polymerase III transcripts associate with RNA polymerase II (Pol II) and represses transcription of mRNA genes. (aacrjournals.org)
  • Moreover, an antisense oligonucleotide against B2 RNA attenuated the transcriptional repression of the actin and hexokinase II genes after heat shock, showing that B2 RNA is critical for the repression of mRNA genes during the mouse heat shock response. (aacrjournals.org)
  • Antisense oligonucleotides directed against Alu RNA relieved repression of several housekeeping genes in heat shocked human cells. (aacrjournals.org)
  • Further cell-based studies to probe the mechanism by which B2 and Alu RNA repress transcription showed that Pol II and the ncRNAs co-occupy the promoters of genes that are transcriptionally repressed in response to heat shock. (aacrjournals.org)
  • To study the prevalence and clinical impact of PRC2 aberrations in an unselected childhood ALL cohort (n = 152), we performed PRC2 mutational screenings by Sanger sequencing and promoter methylation analyses by quantitative pyrosequencing for the three PRC2 core component genes EZH2, suppressor of zeste 12 (SUZ12), and embryonic ectoderm development (EED). (cancerindex.org)
  • Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. (yeastgenome.org)
  • Plastids have an interesting transcription machinery that makes it possible to study the interplay of mono- and multisubunit RNA polymerases (RNAPs) during intricate organelle biogenesis, requiring the concerted expression of genes located in different compartments of the cell. (springer.com)
  • Highly purified pea chloroplast RNA polymerase transcribes both rRNA and mRNA genes. (springer.com)
  • RNA polymerase subunits encoded by the plastid rpo genes are not shared with the nucleus encoded plastid enzyme. (springer.com)
  • parallel loss of the rrn16 PEP-promoter and of the rpoA and rpoB genes coding for the plastid-encoded RNA polymerase. (springer.com)
  • T box riboswitches are RNA regulatory elements widely used by organisms in the phyla Firmicutes and Actinobacteria to regulate expression of amino acid-related genes. (pnas.org)
  • T box riboswitches are cis- acting RNA regulatory elements found in the 5′-untranslated (leader) regions of many essential amino acid-related genes in organisms of the phyla Actinobacteria and Firmicutes ( 1 ). (pnas.org)
  • Infection is initiated by entry of the bacteria into intestinal epithelial cells and is mediated by a type III secretion system that is encoded by genes in Salmonella pathogenicity island 1. (asm.org)
  • These genes encode transcriptional regulators, type III secretion system components, chaperones, and secreted effectors. (asm.org)
  • Human transcription factor IIIC (hTFIIIC) is a multisubunit complex that mediates transcription of class III genes through direct recognition of promoters (for tRNA and virus-associated RNA genes) or promoter-TFIIIA complexes (for the 5S RNA gene) and subsequent recruitment of TFIIIB and RNA polymerase III. (embl-heidelberg.de)
  • Recent technological breakthroughs have enabled high-throughput quantitative measurements of hundreds of thousands of genetic interactions among hundreds of genes in Saccharomyces cerevisiae . (biomedcentral.com)
  • The most extreme example of a negative interaction is synthetic lethality, in which the joint deletion of two nonessential genes leads to a lethal phenotype. (biomedcentral.com)
  • For example, two approaches, systematic genetic analysis (SGA) [ 5 , 6 ] and dSLAM (heterozygote diploid-based synthetic lethality analysis with microarrays) [ 7 , 8 ], have made it possible to screen for negative GIs, namely synthetic sick or synthetic lethal interactions, between a query gene and the collection of all nonessential genes. (biomedcentral.com)
  • S. sclerotiorum inoculation strongly induced the expression of BnCAMTA3 genes while significantly suppressed that of some CGCG-containing RNA silencing component genes, suggesting that RNA silencing machinery might be targeted by CAMTA3. (frontiersin.org)
  • In Drosophila, cohesin is largely absent from silent genes, and selectively binds active genes in which transcriptionally-engaged RNA polymerase II (Pol II) pauses just downstream of the start site [9] , [12] . (prolekare.cz)
  • These promoters may be classified into three groups according to their expression time and functional characteristics of the corresponding genes: class "early" (subgroup of class II), class II and class III (1). (jbsdonline.com)
  • Pol III-dependent transcription of tRNA genes controlled by type 2 promoters requires the transcription factor IIIC (TFIIIC) to bind to two gene-internal elements named the A-box and B-box ( 2 ), thereby inducing the recruitment of the transcription factor IIIB (TFIIIB) and subsequently the recruitment of Pol III ( 3 ). (pubmedcentralcanada.ca)
  • Here we present the cryo-electron microscopy structures of the Saccharomyces cerevisiae Pol III elongating complex at 3.9 Å resolution and the apo Pol III enzyme in two different conformations at 4.6 and 4.7 Å resolution, respectively, which allow the building of a 17-subunit atomic model of Pol III. (rcsb.org)
  • Saccharomyces cerevisiae RPC5 subunit (or RPC40) from RNA polymerases I and III. (ebi.ac.uk)
  • In the yeast Saccharomyces cerevisiae , Maf1 is the only known global and direct Pol III transcription repressor which mediates numerous stress signals. (asm.org)
  • To this end, we used Pol II from the yeast Saccharomyces cerevisiae , which is well suited for structural studies, and a previously described RNA aptamer referred to as 'FC' RNA [ 22 ] that binds and inhibits yeast Pol II in a manner similar to the two natural RNAs described above. (portlandpress.com)
  • This analysis led to the identification of small molecule inhibitors of RNA polymerase (Pol) III from Saccharomyces cerevisiae . (asm.org)
  • In this study we investigate a noncanonical Pol III transcription unit that contains a consensus upstream TATA box element, the Saccharomyces cerevisiae U6 RNA gene SNR6 (Fig. 1 ). (asm.org)
  • RNA polymerase II of Saccharomyces cerevisiae consisting of all 12 subunits. (wikiversity.org)
  • Minaker S, Filiatrault M, Ben Aroya S, Hieter P, Stirling P. Biogenesis of RNA polymerases II and III requires the conserved GPN small GTPases in Saccharomyces cerevisiae. (labome.org)
  • Saccharomyces cerevisiae τ55, a subunit of the RNA polymerase III-specific general transcription factor TFIIIC, comprises an N-terminal histidine phosphatase domain (τ55-HPD) whose catalytic activity and cellular function is poorly understood. (pubmedcentralcanada.ca)
  • Common component of RNA polymerases I and III which synthesize ribosomal RNA precursors and small RNAs, such as 5S rRNA and tRNAs, respectively. (uniprot.org)
  • Common component of RNA polymerases I, II and III which synthesize ribosomal RNA precursors, mRNA precursors and many functional non-coding RNAs, and small RNAs, such as 5S rRNA and tRNAs, respectively. (uniprot.org)
  • RNA polymerase I: located in the nucleoli, synthesises precursors of most ribosomal RNAs. (ebi.ac.uk)
  • RNA polymerase III: also occurs in the nucleoplasm, synthesises the precursors of 5S ribosomal RNA, the tRNAs, and a variety of other small nuclear and cytosolic RNAs. (ebi.ac.uk)
  • Whereas Pol I and Pol II synthesize ribosomal and mainly mRNA respectively, Pol III transcribes small RNAs, including transfer RNAs, 5 S ribosomal RNA and U6 small nuclear RNA. (portlandpress.com)
  • This gene encodes a small essential subunit of RNA polymerase III, the polymerase responsible for synthesizing transfer and small ribosomal RNAs in eukaryotes. (creativebiomart.net)
  • There is growing evidence that synthesis of ribosomal RNA by pol I occurs at the dense fibrillar component and fibrillar center interface. (rupress.org)
  • RNA Pol III synthesizes many small RNAs, including 5S ribosomal rRNA and tRNAs, U6 and RNase P snRNAs, MRP small nucleolar RNA (snoRNA), as well as SRP and the family of Y small cytoplasmic RNAs. (rupress.org)
  • Although most Pol III transcription units lack a consensus TATA box in the TFIIIB-binding region, A/T-rich sequences 15 to 30 base pairs upstream of the start site are known to contribute to the efficiency of tRNA and 5S rRNA gene transcription in vitro ( 29 , 38 ) and in vivo ( 23 , 40 ). (asm.org)
  • The small nucleolar RNAs (snoRNAs) U3, U14, and MRP were also found to be associated with the complex, which supports accurate transcription, termination, and pseudouridylation of rRNA. (rupress.org)
  • Augmented import of RNase P, 5S rRNA, and MRP RNAs depended on PNPASE expression and PNPASE-imported RNA interactions were identified. (ca.gov)
  • Upon flavivirus infection, translation of incoming viral genomic RNA occurs, and replication of the viral RNA begins with the synthesis of minus-strand RNA which then serves as the template for the synthesis of progeny genomic RNA. (asm.org)
  • Many specific sequences as well as structures at the 3′ termini of the genomes of positive-sense RNA viruses have been demonstrated to be important for the synthesis of minus-strand RNA ( 11 , 18 , 22 , 27 , 28 ). (asm.org)
  • RNA synthesis follows after the attachment of RNA polymerase to a specific site, the promoter, on the template DNA strand. (ebi.ac.uk)
  • The RNA synthesis process continues until a termination sequence is reached. (ebi.ac.uk)
  • RNA synthesis in the eukaryote nucleus is carried out by the multisubunit RNA polymerases (Pols) I, II and III. (portlandpress.com)
  • This gene has been shown to function in several processes that include transcriptional regulation, epigenetic remodeling, promotion of cell proliferation, and regulation of RNA synthesis. (genecards.org)
  • Through their interaction with Pol II, the ncRNAs assemble into preinitiation complexes at promoters and block RNA synthesis. (aacrjournals.org)
  • The recruitment of TFBM2 induces DNA unwinding at the promoter and enables initiating nucleotides to be incorporated into the mtDNA/TFAM/POLRMT/TFBM2 ternary complex, thus beginning RNA synthesis. (news-medical.net)
  • Regulates ribosome synthesis by activating RNA polymerase III-dependent transcription through phosphorylation and inhibition of MAF1 a RNA polymerase III-repressor. (ucsc.edu)
  • While the major steps in RNA synthesis have been known for several decades, scientists have only recently begun to decipher the detailed molecular steps of the complex transcription process. (phys.org)
  • Transcription - the transfer of DNA's genetic information through the synthesis of complementary molecules of messenger RNA - forms the basis of all cellular activities. (phys.org)
  • RNA polymerase II: occurs in the nucleoplasm, synthesises mRNA precursors. (ebi.ac.uk)
  • Coincident with changes in mRNA transcription, RNA polymerase III transcription of B2 RNA and Alu RNA is upregulated in response to heat shock (4). (aacrjournals.org)
  • C. Surface representation of the transcription-competent polymerase (from pdb 4WSA) highlighting the position of template exit indicated by the location of the obstructing PB2 helical lid (colored brown), and of the putative mRNA exit tunnel. (jle.com)
  • 13 ] pioneered the prediction of GIs in S. cerevisiae , using probabilistic decision trees and diverse genomic data, including mRNA expression, functional annotations, subcellular localization, deletion phenotypes and physical interactions. (biomedcentral.com)
  • used cryo-electron microscopy, mass spectrometry, and biochemical reconstitutions to show that the mRNA 3′-end processing machinery is organized into nuclease, polymerase, and phosphatase modules. (sciencemag.org)
  • Combined with in vitro reconstitution experiments, our data show that the polymerase module brings together factors required for specific and efficient polyadenylation, to help coordinate mRNA 3′-end processing. (sciencemag.org)
  • CPF interacts with Pol II during transcription, monitoring the nascent pre-mRNA until it recognizes specific RNA elements. (sciencemag.org)
  • Using a tandem affinity purification tag, we purified native CPF from yeast ( Fig. 1A ) that was active and specific in cleavage and polyadenylation assays: CPF cleaves a model CYC1 pre-mRNA in vitro and adds a poly(A) tail onto the 5′ (but not 3′) cleavage product ( Fig. 1B and fig. S1). (sciencemag.org)
  • In eukaryotes, 3′ end cleavage of transcripts generated by RNA polymerase II (pol II) is a universal step of gene expression that proceeds through the recognition of cis -acting elements of the pre-messenger RNA (mRNA) [defined as the poly(A) signal] by a complex machinery. (pubmedcentralcanada.ca)
  • Although cleavage and polyadenylation can be studied as isolated processes in vitro , mRNA 3′ end formation in vivo is an integral component of the coupled network in which the different machines carrying out separate steps of the gene expression pathway are tethered to each other to form a gene expression factory. (pubmedcentralcanada.ca)
  • The functional interdependence between splicing and 3′ end processing is mediated by the molecular link between splicing factors bound at the last intron 3′ splice site and pA factors associated to the poly(A) signal in the terminal exon [( 12-16 ) and references inside] and contributes to define the last exon of a pre-mRNA ( 17 ). (pubmedcentralcanada.ca)
  • RPB1 is the largest subunit of RNA polymerase II. (wikiversity.org)
  • THE carboxy-terminal domain (CTD) of the large subunit of RNA polymerase II (Pol II) consists of 26 (in yeast) to 52 (in humans) repeats of the heptapeptide YSPTSPS ( D ahmus 1996 ). (genetics.org)
  • The RPB9 subunit of RNA polymerase II regulates transcription elongation activity and is required for the action of the transcription elongation factor, TFIIS. (embl-heidelberg.de)
  • Phosphorylation patterns of the C-terminal domain (CTD) of largest subunit of RNA polymerase II (called the CTD code) orchestrate the recruitment of RNA processing and transcription factors. (muni.cz)
  • This finding suggests that transgene-derived small interfering RNAs (siRNAs) circumvented the requirement for MOP1, a predicted RNA-dependent RNA polymerase, and MOP3, the predicted largest subunit of RNA polymerase IV (Pol IV). (genetics.org)
  • The structure shows that the central region of FC RNA binds in the polymerase active-centre cleft above the bridge helix [ 7 ]. (portlandpress.com)
  • Mouse B2 RNA binds to yeast Pol II as tightly as FC RNA, indicating that the B2 RNA-binding site on Pol II is conserved among eukaryotes. (portlandpress.com)
  • Binds to RNA polymerase II (RNAPII) (PubMed:17643375, PubMed:20864038, PubMed:21844196). (genecards.org)
  • We found that B2 RNA binds to core Pol II with low nM affinity. (aacrjournals.org)
  • Human Alu RNA also binds with high affinity and specificity to Pol II, and one molecule of Alu RNA can bind two molecules of Pol II. (aacrjournals.org)
  • In its dephosphorylated state, Maf1p binds the N-terminus of the Rpc160p subunit of Pol III to prevent closed-complex formation. (wikipathways.org)
  • It begins when the RNA polymerase binds with transcription promoting regions of DNA. (phys.org)
  • CAP binds to a region of DNA near the RNA polymerase, which activates lac operon expression. (evolutionnews.org)
  • 23. The method of claim 17, wherein said RNA polymerase II is a genetically modified variant of a naturally occurring enzyme. (freepatentsonline.com)
  • Azuma Y, Yamagishi M, Ishihama A. Subunits of the Schizosaccharomyces pombe RNA polymerase II: enzyme purification and structure of the subunit 3 gene. (ebi.ac.uk)
  • Finally, we obtained the first structural information on Pol III, in the form of an 11-subunit model obtained by combining a homology model of the nine-subunit core enzyme with a new X-ray structure of the subcomplex C17/25. (portlandpress.com)
  • Third, in biochemical assays, UK-118005 inhibits tRNA gene transcription in vitro by the wild-type but not the mutant Pol III enzyme. (asm.org)
  • DNA-directed RNA polymerase II subunit RPB1 is an enzyme that in humans is encoded by the POLR2A gene. (wikiversity.org)
  • RPB2 (POLR2B) is the second largest subunit which in combination with at least two other polymerase subunits forms a structure within the polymerase that maintains contact in the active site of the enzyme between the DNA template and the newly synthesized RNA. (wikiversity.org)
  • RNAP is the key enzyme involved in creating an equivalent RNA copy of a sequence of DNA. (phys.org)
  • Here, we show that in flies, 3′ terminal RNA uridylation triggers the processive, 3′‐to‐5′ exoribonucleolytic decay via the RN ase II /R enzyme CG 16940, a homolog of the human Perlman syndrome exoribonuclease Dis3l2. (embopress.org)
  • The identification of an enzyme complex degrading aberrant RNA in flies deepens our understanding of cytoplasmic RNA surveillance and sheds light on the emerging role for RNA modifications. (embopress.org)
  • Thus, electrostatic patterns of T7 DNA promoters are distinguished owing to specific motifs wich may be involved as signal elements in differential recognition of T7 RNA-polymerase specific promoters by the enzyme. (jbsdonline.com)
  • RPB8 (POLR2H) interacts with subunits RPB1-3, 5, and 7. (wikiversity.org)
  • It interacts with RPB1-3 and 5, and strongly with RPB3. (wikiversity.org)
  • Domain I also interacts with the transcriptionally active RNA polymerase II holoenzyme and therefore, may have a function unrelated to the previously described transcription elongation activity of TFIIS. (embl-heidelberg.de)
  • Yeast two-hybrid screening and bimolecular luciferase complementation experiments revealed that FL3 interacts with the RNA polymerase III subunit 53 (RPC53) and transcription factor class C 1 (TFC1), two critical factors of the RNA polymerase III (RNAPIII) transcription complex. (plantcell.org)
  • Also acts as a transcription activator via its interaction with ZNF143 by participating in efficient U6 RNA polymerase III transcription. (genecards.org)
  • Also acts as a transcription activator via its interaction with ZNF143 by participating to efficient U6 RNA polymerase III transcription. (abcam.com)
  • In addition, an increasing number of Pol II transcription units have been found to contain TFIID-binding promoter elements 20 to 30 base pairs downstream of the transcription start site in a position analogous to that of the Pol III A block element ( 3 , 33 ). (asm.org)
  • In the absence of its normal contacts with the promoter, Pol II is likely held in these inactive complexes on DNA through interactions with TBP, TFIIB, and TFIIF. (aacrjournals.org)
  • Tn 5lacZY expression, suggesting that HilD activates transcription of hilA by contacting and recruiting RNA polymerase to the hilA promoter. (asm.org)
  • RNA promoter binding to the polymerase. (jle.com)
  • All images are generated from the FluB structure pdb 4WRT [15] which is the only crystal containing full-length 5' and 3' vRNA strands used as surrogate of viral promoter. (jle.com)
  • A. Representation of the 5' and 3' vRNA promoter, highlighting the base pairing of the distal region and configuration of single-strand extremities. (jle.com)
  • D. Surface representation of the polymerase promoter-binding interface highlighting the binding 5'vRNA hook in a pocket formed by PB1 and PA. (jle.com)
  • Cartoon representation of PB2 folding in promoter-bound (shown for FluB pdb 4WSA) and in the 5'cRNA bound (FluB pdb 5EPI) polymerase configurations. (jle.com)
  • A. Representation of the structures of the FluB polymerase when bound to the 5' + 3' vRNA viral promoter (pdb 4WSA) or to the 5'cRNA only (pdb 5EPI), and of the apo-FluC polymerase (pdb 5d9). (jle.com)
  • Interestingly, once bound to DNA, TFIID induced dissociation of p53 from the complex to allow additional p53 molecules to escort general transcription factors, such as RNA Polymerase II to the TFIID bound promoter scaffold. (yu.edu)
  • The paper "Promoter melting triggered by bacterial RNA polymerase occurs in three steps" was written by Jie Chen, Seth A. Darst, and D. Thirumalai. (phys.org)
  • Their sequences are characterized by comparatively extended consensus sequence (23 bp) with a high level of homology for all promoters but, despite their considerable sequence similarity, the different promoter classes differ by their strengths and by many other biochemical properties (3, 4). (jbsdonline.com)
  • p>Used to describe "traditional" genetic interactions such as suppressors and synthetic lethals as well as other techniques such as functional complementation, rescue experiments, or inferences about a gene drawn from the phenotype of a mutation in a different gene. (uniprot.org)
  • Functional interaction of the subunit 3 of RNA polymerase II (RPB3) with transcription factor-4 (ATF4). (ebi.ac.uk)
  • It was shown that only the 5′ ε, but not the 3′ ε, is functional for encapsidation, a phenomenon termed position-effect ( 7 , 11 ). (asm.org)
  • Extensive processing of the transcripts is required to produce fully functional RNA components. (news-medical.net)
  • Bacterial RNA polymerase subunit ω and eucaryotic RNA polymerase subunit RPB6 are sequence, structural, and functional homologs and promote RNA polymerase assembly. (springer.com)
  • These 30 amino acids may physically orient domains II and III to support functional interactions between TFIIS and RNA polymerase II. (embl-heidelberg.de)
  • The conserved functional motifs of RNA polymerase lying in the palm domain are colored in purple, the priming loop in red and the PB1 bipartite NLS in rust. (jle.com)
  • Recent studies showed that not only serines and tyrosines but also threonines of the CTD can be phosphorylated with a number of functional consequences, including the interaction with yeast transcription termination factor, Rtt103p. (muni.cz)
  • In crop plants and many vegetables, it may account for as much as 3 to 10% of the dry weight and plays various functional roles in biological and metabolic processes such as metal tolerance, ion balance, and insect defense ( Franceschi and Nakata, 2005 ). (plantcell.org)
  • Several small nuclear and cytoplasmic RNAs are synthesized by RNA polymerase (Pol) III as nascent transcripts that must then migrate through RNA processing, modification, and transport machinery on their trek to becoming mature, functional molecules. (rupress.org)
  • Recent advances in single-molecule imaging provide an unprecedented spatiotemporal window into probing dynamic functional interactions between these heterogeneous large multi-subunit transcription assemblies and chromatin in real-time. (yu.edu)
  • In addition, high-resolution cryo-electron microscopy and genome-wide binding assays allow us to rapidly survey detailed functional interactions on physiologically relevant substrates with limited amounts of samples. (yu.edu)
  • Through a subsequent combination of the focused quantitative proteomics experiments with available large-scale genetic interaction data and Gene Ontology functional associations, we provided sufficient evidence to associate Ydl156w with multiple processes including chromatin remodeling, transcription and DNA repair/replication. (mcponline.org)
  • The averaged profiles of electrostatic potential distribution around T7 RNA-polymerase specific promoters belonging to different functional group. (jbsdonline.com)
  • The physical interconnections between the splicing/transcription and 3′ end processing machineries create a strong functional interdependence. (pubmedcentralcanada.ca)
  • Nevertheless, template specificity of the viral RNA on the NS5B RdRp activity was not observed. (asm.org)
  • However, recent studies indicated that HCV NS5B interacted with the 3′ conserved 98 nt of the viral genome with little specificity ( 23 ) and had no clear preference to utilize the 98-nt RNA as a template in RdRp activity assay ( 38 ). (asm.org)
  • These results suggest that C34 and TFIIIB 70 are specificity determinants of the RNA polymerase III-TFIIIB interaction. (elsevier.com)
  • Fcp1 specificity may additionally arise from phosphatase recruitment near the CTD via the Pol II subcomplex Rpb4/7, which is shown to be required for binding of Fcp1 to the polymerase in vitro. (rcsb.org)
  • These small RNAs are then incorporated into AGO-containing RNA-induced silencing complexes (RISCs) to serve as the sequence specificity in RNA degradation, translational inhibition, or heterochromatin formation ( Bologna and Voinnet, 2014 ). (frontiersin.org)
  • In this network, 3′ end processing cross-talks with the transcription and splicing steps to optimize the efficiency and specificity of each enzymatic reaction ( Figure 1 ) (1). (pubmedcentralcanada.ca)
  • First, a dominant mutation in the g domain of Rpo31p, the largest subunit of RNA Pol III, confers resistance to the compound. (asm.org)
  • RNA polymerase II subunit B4 (RPB4) encoded by the POLR2D gene [7] is the fourth largest subunit and may have a stress protective role. (wikiversity.org)
  • The NS5B-RNA complexes were specifically competed away by the unlabeled homologous RNA but not by the viral 5′ noncoding region and very poorly by the 3′ conserved 98-nucleotide tail. (asm.org)
  • Interactions with other relevant participants such as small molecules (purple), sub-complexes (yellow), and other subunits (red) are also shown. (yeastgenome.org)
  • However, all pol I-containing complexes described so far failed to show RNA processing activities. (rupress.org)
  • Once B2 RNA or Alu RNA is removed from preinitiation complexes, transcriptional activity is restored. (aacrjournals.org)
  • We conclude that B2 and Alu RNA prevent Pol II from properly engaging the DNA, resulting in complexes with an altered conformation that are transcriptionally inert. (aacrjournals.org)
  • We have previously solved the structures of domains II and III, which stimulate arrested polymerase II elongation complexes in order to resume transcription. (embl-heidelberg.de)
  • The overall shape of the polymerase complexes in four resolved structures are shown with a fixed position of the central core. (jle.com)
  • To study the relevance of this interaction for virus replication we mutated the PB2 NLS and analysed the phenotype of mutant subunits, polymerase complexes and RNPs. (nih.gov)
  • Elongin A was isolated by virtue of its ability to increase the catalytic rate of transcript elongation by RNA polymerase II in vitro [16]. (wikiversity.org)
  • In vitro studies to directly probe the mechanism by which B2 RNA and Alu RNA repress transcription took advantage of a highly purified reconstituted Pol II transcription system. (aacrjournals.org)
  • We found that human scAlu RNA and mouse B1 RNA also bind Pol II, but do not repress transcription in vitro. (aacrjournals.org)
  • Affinity purification of the tobacco plastid RNA polymerase and in vitro reconstitution of the holoenzyme. (springer.com)
  • In vitro biochemical assays revealed that p53 stimulates transcription elongation of RNA Polyermase II suggesting a common structural mechanism associated with regulating elongation. (yu.edu)
  • However, RB remains able to regulate Pol III transcription in the presence of the histone deacetylase inhibitor trichostatin A. Instead, RB represses by disrupting interactions between TFIIIB and other components of the basal Pol III transcription apparatus. (hud.ac.uk)
  • In addition, RB disrupts the interaction between TFIIIB and Pol III that is essential for transcription. (hud.ac.uk)
  • The interaction that occurs between the bait and the prey (containing τ138p) results in binding of the TFIIIC complex to DNA and recruitment of TFIIIB (a second transcription factor) and Pol III. (news-medical.net)
  • The discussion of the polymerase III transcription machinery first focuses on the two traditional factor fractions TFIIIB and TFIIIC and then on individual components recently resolved from these fractions. (asmscience.org)
  • Previous studies also demonstrated a specific binding of encephalomyocarditis virus RNA polymerase to the viral poly(A)-containing 3′NCR ( 8 , 9 ). (asm.org)
  • 2',5'-0ligo(A): A Mediator of Viral RNA Cleavage in Interferon-Treated Cells? (elsevier.com)
  • Recognition of viral pathogen-associated molecular patterns such as viral RNAs or DNAs by the pathogen recognition receptors triggers signaling cascades, ultimately leading to the activation of IRF3 and IRF7 that involves phosphorylation and nuclear accumulation of the two factors. (jimmunol.org)
  • STUDY DESIGN: The complete HCV genome was reverse transcribed with a pan-genotype primer binding at the 3'end of the viral RNA. (bireme.br)
  • The viral RNA replicates in the cytoplasm of infected hepatocytes in a specialized convoluted structure called "membranous web" derived from the membrane of endoplasmic reticulum [ 10 - 12 ]. (hindawi.com)
  • Defects in 3′-end processing occur in human diseases, including β-thalassemia, thrombophilia, and cancer, as well as viral infections ( 8 - 10 ). (sciencemag.org)
  • The amino acid sequence of the human RNA polymerase II 33-kDa subunit hRPB 33 is highly conserved among eukaryotes. (ebi.ac.uk)
  • Together with high‐throughput biochemical characterization of dmDis3l2 and bacterial RN ase R, our results imply a conserved molecular function of RN ase II /R enzymes as "readers" of destabilizing posttranscriptional marks-uridylation in eukaryotes and adenylation in prokaryotes-that play important roles in RNA surveillance. (embopress.org)
  • 265, 17816-17819], one of the five polypeptides common to all three nuclear RNA polymerases. (pnas.org)
  • Small GTPase required for proper nuclear import of RNA polymerase II (RNAPII) (PubMed:20855544, PubMed:21768307). (genecards.org)
  • These 3' RNA-processing components colocalized with FCA in the nuclear bodies in vivo, which indicates that FCA nuclear bodies compartmentalize 3'-end processing factors to enhance polyadenylation at specific sites. (jic.ac.uk)
  • Although most La is nucleoplasmic, some is nucleolar, and several Pol III transcripts, including precursors of tRNAs, U6 small nuclear RNA (snRNA), RNase P RNA, and signal recognition particle (SRP) RNA, have recently been shown to move through the nucleolus. (rupress.org)
  • Here, we show a new role for PNPASE in regulating the import of nuclear-encoded RNAs into the mitochondrial matrix. (ca.gov)
  • These results indicate that, above and beyond its role in nuclear accumulation, PB2 interaction with alpha-importins is required for virus RNA replication. (nih.gov)
  • 3′ end processing is a nuclear co-transcriptional process that promotes transport of mRNAs from the nucleus to the cytoplasm and affects the stability and the translation of mRNAs. (pubmedcentralcanada.ca)
  • Hepatitis C virus (HCV) is an enveloped virus that possesses a single-stranded positive-sense RNA genome encoding a polyprotein of approximately 3,000 amino acid residues ( 7 , 20 , 29 ). (asm.org)
  • In humans, telomerase is composed of a reverse transcriptase (hTERT), which uses the RNA component (hTERC) to dock onto the 3′ single-stranded telomere end. (nature.com)
  • It contains a positive sense single-stranded RNA genome of about 9500 nucleotides that is composed of 5′- and 3′ noncoding regions (NCR) serving important regulatory functions during replication. (hindawi.com)
  • Dephosphorylation allows translocation of Maf1p to the nucleus and nucleolus under stress conditions, which shuts down RNA Pol III transcription.Phosphorylation of Maf1p by CK2 occurs at promoters, which releases Maf1p from chromatin, liberating RNA Pol III from inhibition. (wikipathways.org)
  • Electrostatic profiles of the individual promoters were combined into three corresponding groups and the averaged electrostatic profiles of these separate groups were calculated as indicated in (6). (jbsdonline.com)
  • The profiles of the promoters can be characterized by the presence of particular electrostatic elements in the region from -25 bp to + 20 bp wich is known to be involved in contacts with T7 RNA-polymerase. (jbsdonline.com)
  • In contrast, the promoters belonging to class III has the only most expressed negative fall at ~ -18 bp and two peaks downstream at ~ + 5 bp and ~ +20 bp. (jbsdonline.com)
  • A recently discovered mechanism for gene regulation involves non-coding RNAs that bind and inhibit cellular RNA polymerases. (portlandpress.com)
  • Our results reveal the importance of RNA silencing in plant resistance to S. sclerotiorum and imply a new mechanism of CAMTA function as well as RNA silencing regulation. (frontiersin.org)
  • Our results provide novel insights into Pol III-specific transcription and the adaptation of Pol III towards its small transcriptional targets. (rcsb.org)
  • Namely, we found that mouse B2 RNA and human Alu RNA are trans-acting transcriptional repressors of Pol II (1-3). (aacrjournals.org)
  • Transcriptional activities of the chloroplast-nuclei and proplastid-nuclei isolated from tobacco exhibit different sensitivities to tagetitoxin: Implication of the presence of distinct RNA polymerases. (springer.com)
  • TOR signaling controls all three RNA polymerase systems involved in ribosome biogenesis ( 22 , 39 , 42 ). (asm.org)
  • They are located within coding region transcribed by the host E. coli RNA-polymerase. (jbsdonline.com)
  • It is phosphorylated by casein kinase 2 during mitosis, resulting in its release from chromatin and suppression of polymerase III transcription. (wikipedia.org)
  • Involved in both enhancer blocking and epigenetic remodeling at chromatin boundary via its interaction with CTCF. (genecards.org)
  • Early studies of Spt4-Spt5 led to the model that it affects transcription through interactions with chromatin ( S wanson and W inston 1992 ). (genetics.org)
  • Nucleosomes are highly dynamic structures, which are responsible for the state of chromatin, and may be found in either an extended (accessible = euchromatin) or condensed, (tightly packed = heterochromatin) state ( 3 ). (mcponline.org)
  • To clarify cohesin's roles in transcription, we measured how cohesin controls RNA polymerase II (Pol II) activity by genome-wide chromatin immunoprecipitation and precision global run-on sequencing. (prolekare.cz)
  • The 3′ noncoding region (3′NCR) consists of a short genotype-specific region and a poly(U)-C(U) n repeat stretch of variable length followed by a highly conserved tail of 98 nucleotides (nt) ( 12 , 21 , 30 , 37 ). (asm.org)
  • Similar to transcripts of Pols I and II, newly synthesized Pol III transcripts are subjected to a maturation process that involves removal, modification, and addition of nucleotides. (rupress.org)
  • DCLs undergo RNase III-type activities to process complementary double-strand RNAs into small RNAs with 21-26 nucleotides in length ( Carmell and Hannon, 2004 ). (frontiersin.org)
  • The posttranscriptional addition of nucleotides to the 3′ end of RNA regulates the maturation, function, and stability of RNA species in all domains of life. (embopress.org)
  • Among these, the addition of non‐templated nucleotides to the 3′ hydroxyl end of RNA (i.e. tailing) is one of the most frequent marks, exhibiting deep conservation and serving diverse molecular functions. (embopress.org)
  • Studies to understand the molecular mechanism of HCV replication have been restricted by the lack of a well-established cell culture system, but studies from other positive-sense RNA viruses may provide some clues. (asm.org)
  • These annotations have been derived from physical molecular interaction evidence extracted from the literature and cross-referenced in the entry, or by curator inference from information on homologs in closely related species or by inference from scientific background. (yeastgenome.org)
  • Using classical genetics, molecular biology, and biochemistry, we show that UK-118005 inhibits RNA polymerase (Pol) III. (asm.org)
  • however, the molecular basis of such an interaction has still to be elucidated. (rupress.org)
  • GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies ( Molecular Function , Biological Process , and Cellular Component ), a reference, and an evidence code. (yeastgenome.org)
  • Evidence that this molecular chaperone accompanies precursor tRNAs through various RNA modification and processing events and facilitates the assembly of other transcripts into specific RNPs is reviewed. (rupress.org)
  • Knowledge of the molecular changes underpinning adaptations allow new insight into the constraints on, and repeatability of adaptations, and of the basis of non-additive interactions between adaptive mutations. (portlandpress.com)
  • These results suggest that the assembly of RNA polymerase I and III requires the association of ABC10 beta subunit with an AC19/AC40 heterodimer. (pnas.org)
  • The structures are also used to identify molecules that bind to or otherwise interact with structural elements in the polymerase. (freepatentsonline.com)
  • The identification of these inhibitors demonstrates that RNA Pol III can be targeted by small synthetic molecules. (asm.org)
  • PIKKs are large molecules (270 to 470 kD) with a conserved kinase domain, a FAT-C (FRAP, ATM, TRRAP C-terminal) domain, and multiple helical repeats ( 1 , 3 ). (sciencemag.org)
  • Two molecules of this subunit are present in each RNA polymerase II. (wikiversity.org)
  • A 3′ coding region with conserved stem-loop structures rather than the 3′ noncoding region of the HCV genome is critical for the specific binding of NS5B. (asm.org)
  • Retrotransposons are repetitive DNA elements in the genome that can replicate and insert a new copy into the genome via an RNA intermediate. (ubc.ca)
  • The DNA genome of hepatitis B virus (HBV) replicates via reverse transcription within capsids following the encapsidation of an RNA template, the pregenomic RNA (pgRNA). (asm.org)
  • Although they contain a DNA genome, hepadnaviruses replicate via the reverse transcription of an RNA template, the pregenomic RNA (pgRNA) ( 11 ). (asm.org)
  • Does RNA polymerase transcribe the entire genome? (brainscape.com)
  • Replicative DNA polymerases are nano-machines essential to life, which have evolved the ability to copy the genome with high fidelity and high processivity. (portlandpress.com)
  • In Bacteria, the genome is replicated by DNA polymerases belonging to the A- and C-families. (portlandpress.com)
  • RNA import into mammalian mitochondria is considered essential for replication, transcription, and translation of the mitochondrial genome but the pathway(s) and factors that control this import are poorly understood. (ca.gov)
  • Given high strain-to-strain variability in Ty location and the high aggregate burden of Ty-proximal DSBs, we propose that meiotic recombination is an important component of host-Ty interactions and that Tys play critical roles in genome instability and evolution in both inbred and outcrossed sexual cycles. (prolekare.cz)
  • The core RNA polymerase complex consists of five subunits (two alpha, one beta, one beta-prime and one omega) and is sufficient for transcription elongation and termination but is unable to initiate transcription. (ebi.ac.uk)
  • Indeed, 3′ end polyadenylation factors (or pA factors, including factors involved in both cleavage and polyadenylation) and sequence elements of the poly(A) signal modulate transcription termination ( 2-5 ) and, in turn, transcription factors/activators affect processing at the poly(A) signal ( 6-9 ). (pubmedcentralcanada.ca)
  • Plays a critical role in the phosphorylation at 'Ser-473' of AKT1, a pro- survival effector of phosphoinositide 3-kinase, facilitating its activation by PDK1. (ucsc.edu)
  • In stress conditions, the surface serine patch undergoes phosphorylation, which impairs the interaction of Rrn3p with RNA Pol I, repressing Pol I transcription, and thereby also reducing ribosome production and cell growth. (wikipathways.org)
  • Interaction of Sfp1p with TorC1 reduces Sch9p phosphorylation, resulting in a negative feedback loop. (wikipathways.org)
  • Modifications found previously on La-associated U6 snRNA, coupled with recent data, now suggest that these modifications occur in the nucleolus, while U6 RNA is associated with La. Finally, data which indicate that human La exhibits a two-step mode of RNA binding that is sensitive to phosphorylation are reviewed, and the implications of this mode of binding are considered in the context of the dynamics of RNA maturation. (rupress.org)
  • These newly identified RNA contacts add information about tRNA recognition by the T box riboswitch and demonstrate a role for the S-turn and pseudoknot elements, which resemble structural elements that are common in many cellular RNAs. (pnas.org)
  • In contrast with cellular transcriptases and ribosome machines, which evolved by accretion of complexity from a conserved catalytic core, no replicative DNA polymerase is universally conserved. (portlandpress.com)
  • Human tumorigenesis is a complicated process marked by a loss of the cell's ability to regulate critical cellular processes, such as transcription, RNA processing and translation, leading to uncontrollable cell growth. (yu.edu)
  • Many of these processes are mediated by extensive and intimate interactions of actin with cellular membranes . (wikipedia.org)
  • Although natural products have been identified that inhibit different RNA Pols, such as α-amanitin ( 32 , 33 ) and tagetitoxin ( 34 , 35 ), this is the first example of a synthetic small molecule inhibiting RNA Pol. (asm.org)
  • 5FJ8: Cryo-EM structure of yeast RNA polymerase III elongation complex at 3. (rcsb.org)
  • The core RNA polymerase complex forms a "crab claw"-like structure with an internal channel running along the full length [ PMID: 10499798 ]. (ebi.ac.uk)
  • In addition, Lar7 is required to stabilise the association of telomerase RNA with the protective complex LSm2-8, and telomerase reverse transcriptase. (nature.com)
  • Understanding how these RNAs inhibit transcription requires their three-dimensional structures in complex with the target polymerases. (portlandpress.com)
  • The crystal structure of yeast Pol II in complex with the central region of FC RNA ( Figure 1 ), together with biochemical data and published results, suggests the inhibitory mechanisms of polymerase-binding RNAs [ 23 ]. (portlandpress.com)
  • Topological considerations suggest that FC RNA prevents DNA entry into the cleft during open complex formation. (portlandpress.com)
  • In a preformed elongation complex, however, nucleic acids exclude FC RNA from the cleft, since they are tightly bound to an overlapping, but not identical site. (portlandpress.com)
  • Lar7 stabilises the interaction between TER1 and the Lsm2-8 complex and facilitates assembly with Trt1. (wikiversity.org)
  • The polymerase module of the complex acts as a hub to bring the RNA substrate and the accessory factors together to achieve efficient and controlled polyadenylation coordinated with transcription. (sciencemag.org)
  • Based on genetic and biochemical evidence, we propose a key function of the TRUMP complex in the cytoplasmic quality control of RNA polymerase III transcripts. (embopress.org)
  • This work reports the identification of a cytoplasmic terminal RNA uridylation‐mediated processing ( TRUMP ) complex in Drosophila . (embopress.org)
  • The TRUMP complex regulates precursor‐micro RNA maturation in flies. (embopress.org)
  • The TRUMP complex functions in the quality control of RNA polymerase III transcripts. (embopress.org)
  • The phosphorylated carboxyl-terminal domain (CTD) of pol II also plays a major role in this coupling network by serving as a gathering/delivering platform of pA factors and is an integral component of the 3′ end processing complex (10, 11 ). (pubmedcentralcanada.ca)
  • PNPASE regulates RNA import into mitochondria. (ca.gov)
  • Interestingly, the conserved motifs of RNA-dependent RNA polymerase for putative RNA binding (220-DxxxxD-225) and template/primer position (282-S/TGxxxTxxxNS/T-292) are present in the RBD2. (asm.org)
  • We show that Lar7 has conserved RNA-recognition motifs, which bind telomerase RNA to protect it from exosomal degradation. (nature.com)
  • It is structurally similar to both the T3 and T7 bacteriophage RNA polymerases, and all three have a conserved catalytic core at the C-terminus. (news-medical.net)
  • The catalytic core of influenza polymerase. (jle.com)
  • B. Surface view of the human-fluB polymerase conformation (pdb 4WSA) highlighting the orientation of a cap bound to the PB2 cap binding site facing the catalytic center. (jle.com)
  • 20. The method of claim 17, wherein said RNA polymerase II is bound to an agent. (freepatentsonline.com)
  • A. Structures of 5' + 3' vRNA bound polymerases bat FluA (pdb 4WSB) and human FluB (pdb 4WSA), shown with the PB2-C and PA-ENDO domains in ribbon diagram and the rest of the polymerase in a surface view. (jle.com)
  • We have also determined the 3D cryo-EM structure of p53 bound to RNA Polymerase II. (yu.edu)
  • In this structure p53 occupies a position on RNA Polymerase that is typically bound by elongation factors. (yu.edu)
  • Some Pol III transcripts undergo little, while others undergo extensive, processing. (rupress.org)
  • PNPASE reduction impaired mitochondrial RNA processing and polycistronic transcripts accumulated. (ca.gov)
  • The carboxy-terminal domain of this subunit shares a high degree of sequence similarity to the carboxy-terminal domain of an RNA polymerase II elongation factor. (creativebiomart.net)
  • It contains a carboxy terminal domain (CTD) composed of heptapeptide repeats that are essential for polymerase activity. (wikiversity.org)
  • RNA polymerase III (Pol III) produces essential components of the biosynthetic machinery, and therefore its activity is tightly coupled with cell growth and metabolism. (asm.org)
  • Crosslinking with formaldehyde captured in vivo interactions between FLL2, FCA and the polymerase and nuclease modules of the RNA 3'-end processing machinery. (jic.ac.uk)
  • Nevertheless, RNA silencing machinery in the important oil crop Brassica napus and function in resistance to the devastating fungal pathogen Sclerotinia sclerotiorum are not well-understood. (frontiersin.org)
  • In this study, gene families of RNA silencing machinery in B. napus were identified and their role in resistance to S. sclerotiorum was revealed. (frontiersin.org)
  • DCL, AGO, and RDR are key components of RNA silencing machinery. (frontiersin.org)
  • The 3′-end processing machinery includes the highly conserved cleavage and polyadenylation factor (CPF in yeast, CPSF in metazoans) (table S1). (sciencemag.org)
  • In addition, the 3′ terminus of the flavivirus genomic RNA forms a conserved pseudoknot structure ( 26 ). (asm.org)
  • In this study, by performing a competitive electrophoretic mobility shift assay (EMSA), we have identified conserved stem-loop structures in the 3′ coding region of HCV genomic RNA important for the binding of NS5B RNA polymerase. (asm.org)
  • In Eukarya, genomic DNA is copied mainly by three distinct replicative DNA polymerases, Polα, Polδ, and Polε, which all belong to the B-family. (portlandpress.com)
  • Here we present a novel method, which combines genetic interaction data together with diverse genomic data, to quantitatively impute these missing interactions. (biomedcentral.com)
  • Zhong and Sternberg [ 19 ] used similar ideas and combined diverse genomic information from three species to predict synthetic lethal interactions in Caenorhabditis elegans using a logistic regression classifier. (biomedcentral.com)
  • The AC40 and AC19 subunits (encoded by RPC40 and RPC19) are shared by yeast RNA polymerases I and III and have a local sequence similarity to prokaryotic alpha subunits. (pnas.org)
  • Yeast RNA polymerase III transcription factors and effectors. (yeastgenome.org)
  • Yeast RNA polymerase II subunit RPB9. (embl-heidelberg.de)
  • Because the sequence, structure, and function of multi-subunit RNA polymerase are universally conserved in all organisms -- from bacteria to humans -- understanding the mechanisms of bacterial gene transcription is an important step in deciphering the role of genetics in disease. (phys.org)
  • Portland Press Limited on behalf of the Biochemical Society 2019 classification DNA polymerases DNA replication evolution PolD proofreading DNA replication is one of the most important functions in living organisms and viruses. (portlandpress.com)
  • Strikingly, four different families of DNA polymerases have evolved to perform DNA replication in the three domains of life. (portlandpress.com)
  • Recent structural studies have shown that the structures of both polymerase and proofreading active sites of PolD differ from other structurally characterized DNA polymerases, thereby extending the repertoire of folds known to perform DNA replication. (portlandpress.com)
  • The influenza virus polymerase is formed by the PB1, PB2 and PA subunits and is required for virus transcription and replication in the nucleus of infected cells. (nih.gov)
  • Furthermore, determination of recombinant RNP accumulation in vivo indicated that PB2 NLS mutations drastically reduced virus RNA replication. (nih.gov)
  • The respiratory phenotype of maf1 -Δ allowed genetic suppression studies to dissect the mechanism of Maf1 action on the Pol III transcription apparatus. (asm.org)
  • We find a similar set of genetic interactions between the CTD, CTD modifying enzymes, and TFIIS. (genetics.org)
  • However, these assays often fail to measure the genetic interactions among up to 40% of the studied gene pairs. (biomedcentral.com)
  • Given the number of macromolecules involved in class III transcription, genetic approaches are likely to be essential for identifying all of the players. (asmscience.org)
  • This represents an example of tRNA recognition by an S-turn motif or pseudoknot element, contributing to the understanding of alternative roles for tRNA and other RNA-RNA interactions. (pnas.org)
  • The structure reveals a direct recognition of the phospho-Thr4 mark by Rtt103p CID and extensive interactions involving residues from three repeats of the CTD heptad. (muni.cz)
  • TFIIS is a transcription elongation factor that consists of three domains. (embl-heidelberg.de)
  • DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. (uniprot.org)
  • S. cerevisiae RPB3 subunit from RNA polymerase II. (ebi.ac.uk)
  • Maf1 of S. cerevisiae specifically inhibits Pol III transcription ( 25 ), whereas in human glioblastoma cells, Maf1 is a negative regulator of both Pol I-dependent and Pol III-dependent transcription ( 14 ). (asm.org)
  • The ability of RB to inhibit these key interactions can explain its action as a potent repressor of class III gene expression. (hud.ac.uk)
  • Without bait-prey interaction, the activation domain cannot restrict to the reporter gene-to-gene expression drive. (news-medical.net)
  • RNA silencing is an important mechanism to regulate gene expression and antiviral defense in plants. (frontiersin.org)
  • In plants, RNA silencing is triggered by double-stranded RNA (dsRNA) that gives rise to small RNAs known as microRNAs (miRNAs) or small-interfering RNAs (siRNAs). (frontiersin.org)
  • Mammalian RPB3 (or RPB33) (gene POLR2C) from RNA polymerase II. (ebi.ac.uk)
  • These results showed that whereas some analogs inhibit RNA Pol III as expected, others caused growth inhibition by an entirely different mechanism. (asm.org)
  • Here, we show that the nuclease, polymerase, and phosphatase activities of yeast CPF are organized into three modules. (sciencemag.org)