Porins are protein molecules that were originally found in the outer membrane of GRAM-NEGATIVE BACTERIA and that form multi-meric channels for the passive DIFFUSION of WATER; IONS; or other small molecules. Porins are present in bacterial CELL WALLS, as well as in plant, fungal, mammalian and other vertebrate CELL MEMBRANES and MITOCHONDRIAL MEMBRANES.
Proteins isolated from the outer membrane of Gram-negative bacteria.
A serotype of Salmonella enterica that is a frequent agent of Salmonella gastroenteritis in humans. It also causes PARATYPHOID FEVER.
Proteins found in any species of bacterium.
A family of voltage-gated eukaryotic porins that form aqueous channels. They play an essential role in mitochondrial CELL MEMBRANE PERMEABILITY, are often regulated by BCL-2 PROTO-ONCOGENE PROTEINS, and have been implicated in APOPTOSIS.
A rapid-growing, nonphotochromogenic species of MYCOBACTERIUM originally isolated from human smegma and found also in soil and water. (From Dorland, 28th ed)
A quality of cell membranes which permits the passage of solvents and solutes into and out of cells.
A foul-smelling diamine formed by bacterial decarboxylation of lysine.
A species of gram-negative rod-shaped bacteria found ubiquitously and formerly called Comamonas acidovorans and Pseudomonas acidovorans. It is the type species of the genus DELFTIA.
Gated, ion-selective glycoproteins that traverse membranes. The stimulus for ION CHANNEL GATING can be due to a variety of stimuli such as LIGANDS, a TRANSMEMBRANE POTENTIAL DIFFERENCE, mechanical deformation or through INTRACELLULAR SIGNALING PEPTIDES AND PROTEINS.
Four-membered cyclic AMIDES, best known for the PENICILLINS based on a bicyclo-thiazolidine, as well as the CEPHALOSPORINS based on a bicyclo-thiazine, and including monocyclic MONOBACTAMS. The BETA-LACTAMASES hydrolyze the beta lactam ring, accounting for BETA-LACTAM RESISTANCE of infective bacteria.
Layers of lipid molecules which are two molecules thick. Bilayer systems are frequently studied as models of biological membranes.
A genus of gram-negative bacteria widely distributed in fresh water as well as marine and hypersaline habitats.
A species of gram-negative, aerobic BACTERIA. It is a commensal and pathogen only of humans, and can be carried asymptomatically in the NASOPHARYNX. When found in cerebrospinal fluid it is the causative agent of cerebrospinal meningitis (MENINGITIS, MENINGOCOCCAL). It is also found in venereal discharges and blood. There are at least 13 serogroups based on antigenic differences in the capsular polysaccharides; the ones causing most meningitis infections being A, B, C, Y, and W-135. Each serogroup can be further classified by serotype, serosubtype, and immunotype.
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
Proteins obtained from ESCHERICHIA COLI.
The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.
A genus of gram-negative, aerobic, coccoid bacteria whose organisms are part of the normal flora of the oropharynx, nasopharynx, and genitourinary tract. Some species are primary pathogens for humans.
Gram-negative gas-producing rods found in feces of humans and other animals, sewage, soil, water, and dairy products.
A serotype of SALMONELLA ENTERICA which is the etiologic agent of TYPHOID FEVER.
Any of the processes by which cytoplasmic or intercellular factors influence the differential control of gene action in bacteria.
Substances that reduce the growth or reproduction of BACTERIA.
Detailed account or statement or formal record of data resulting from empirical inquiry.
A species of gram-negative, facultatively anaerobic, rod-shaped bacteria (GRAM-NEGATIVE FACULTATIVELY ANAEROBIC RODS) commonly found in the lower part of the intestine of warm-blooded animals. It is usually nonpathogenic, but some strains are known to produce DIARRHEA and pyogenic infections. Pathogenic strains (virotypes) are classified by their specific pathogenic mechanisms such as toxins (ENTEROTOXIGENIC ESCHERICHIA COLI), etc.
Family of gram-positive, facultatively anaerobic bacteria, in the order Bacillales. Genera include Gemella, Macrococcus, Salinicoccus, and STAPHYLOCOCCUS.
The formation of crystalline substances from solutions or melts. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)
The study of crystal structure using X-RAY DIFFRACTION techniques. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)
A species of gram-negative, aerobic, rod-shaped bacteria commonly isolated from clinical specimens (wound, burn, and urinary tract infections). It is also found widely distributed in soil and water. P. aeruginosa is a major agent of nosocomial infection.
A genus of gram-negative, aerobic, rod-shaped bacteria widely distributed in nature. Some species are pathogenic for humans, animals, and plants.
Infections with bacteria of the genus PSEUDOMONAS.
A species of gram-negative, aerobic bacteria isolated from soil and water as well as clinical specimens. Occasionally it is an opportunistic pathogen.
A species of nonpathogenic fluorescent bacteria found in feces, sewage, soil, and water, and which liquefy gelatin.
A TETRACYCLINE analog, having a 7-dimethylamino and lacking the 5 methyl and hydroxyl groups, which is effective against tetracycline-resistant STAPHYLOCOCCUS infections.
Any tests that demonstrate the relative efficacy of different chemotherapeutic agents against specific microorganisms (i.e., bacteria, fungi, viruses).
Bacteria which retain the crystal violet stain when treated by Gram's method.
Substances that prevent infectious agents or organisms from spreading or kill infectious agents in order to prevent the spread of infection.
One of the three domains of life (the others being Eukarya and ARCHAEA), also called Eubacteria. They are unicellular prokaryotic microorganisms which generally possess rigid cell walls, multiply by cell division, and exhibit three principal forms: round or coccal, rodlike or bacillary, and spiral or spirochetal. Bacteria can be classified by their response to OXYGEN: aerobic, anaerobic, or facultatively anaerobic; by the mode by which they obtain their energy: chemotrophy (via chemical reaction) or PHOTOTROPHY (via light reaction); for chemotrophs by their source of chemical energy: CHEMOLITHOTROPHY (from inorganic compounds) or chemoorganotrophy (from organic compounds); and by their source for CARBON; NITROGEN; etc.; HETEROTROPHY (from organic sources) or AUTOTROPHY (from CARBON DIOXIDE). They can also be classified by whether or not they stain (based on the structure of their CELL WALLS) with CRYSTAL VIOLET dye: gram-negative or gram-positive.
Bacteria which lose crystal violet stain but are stained pink when treated by Gram's method.
Under conditions of starvation, especially the lack of carbon and nitrogen sources, a single endospore forms within some of the bacteria. The process is called sporulation.[13] When a bacterium detects environmental conditions are becoming unfavourable it may start the process of endosporulation, which takes about eight hours. The DNA is replicated and a membrane wall known as a spore septum begins to form between it and the rest of the cell. The plasma membrane of the cell surrounds this wall and pinches off to leave a double membrane around the DNA, and the developing structure is now known as a forespore. Calcium dipicolinate, the calcium salt of dipicolinic acid, is incorporated into the forespore during this time. The dipicolinic acid helps stabilize the proteins and DNA in the endospore.[14]:141 Next the peptidoglycan cortex forms between the two layers and the bacterium adds a spore coat to the outside of the forespore. In the final stages of endospore formation the newly forming ...
The Calcium/Calmodulin binding to the C-terminal pre-IQ domain of the Cav1.2 channel promotes interaction between channels that ... "Normalization of current kinetics by interaction between the alpha 1 and beta subunits of the skeletal muscle dihydropyridine- ...
Interactions[edit]. T4SS has two effector proteins: firstly, ATS-1, which stands for Anaplasma translocated substrate 1, and ... 2011). "Structural analysis of autotransporter components and the origin of porins". Int J Mol Biochem. 29 (13): 92-112.. CS1 ... Some researchers believe remnants of the autotransporters gave rise to the porins which form similar beta-barrel structures.[17 ... Kuehn MJ, Kesty NC (November 2005). "Bacterial outer membrane vesicles and the host-pathogen interaction". Genes & Development ...
A method has been developed to analyze full sets of protein interactions using a combination of 454 pyrosequencing and an in ... Protein nanopore sequencing utilizes membrane protein complexes such as α-hemolysin, MspA (Mycobacterium smegmatis Porin A) or ... DNA-protein interactions (ChIP-sequencing), and epigenome characterization.[55] Resequencing is necessary, because the genome ...
InteractionsEdit. Protein-protein interactionsEdit. TLR 2 has been shown to interact with TLR 1[13] and TOLLIP.[14] ... Porin. Neisseria meningitidis, Haemophilus influenzae Antigen mixtures. Cutibacterium acnes LcrV. Yersinia Lipomannan. ... Protein-ligand interactionsEdit. TLR2 resides on the plasma membrane where it responds to lipid-containing PAMPs such as ... It does this by forming dimeric complexes with either TLR 1 or TLR6 on the plasma membrane.[15] TLR2 interactions with malarial ...
Mutations in genes coding for transpeptidases that lead to reduced interactions with an antibiotic are a significant source of ...
The bacterial phyla are the major lineages, known as phyla or divisions, of the domain Bacteria. In the scientific classification established by Carl von Linné,[2] each bacterial strain has to be assigned to a species (binary nomenclature), which is a lower level of a hierarchy of ranks. Currently, the most accepted mega-classification system is under the three-domain system, which is based on molecular phylogeny. In that system, bacteria are members of the domain Bacteria[3] and "phylum" is the rank below domain, since the rank "kingdom" is disused at present in bacterial taxonomy.[4][Note 1] When bacterial nomenclature was controlled under the Botanical Code, the term division was used, but now that bacterial nomenclature (with the exception of cyanobacteria) is controlled under the Bacteriological Code, the term phylum is preferred. In this classification scheme, Bacteria is (unofficially)[Note 2] subdivided into 30 phyla with representatives cultured in a lab.[5][6][7] Many major clades of ...
Chu X, Tong Q, Cheung JY, Wozney J, Conrad K, Mazack V, Zhang W, Stahl R, Barber DL, Miller BA (March 2004). "Interaction of ...
This ability to thrive in harsh conditions is a result of their hardy cell walls that contain porins. Their resistance to most ... Molecular Plant-Microbe Interactions. 1 (4): 407-11. doi:10.1094/MPMI-4-407.. ...
Interactions[edit]. Products containing multivalent cations, such as aluminium- or magnesium-containing antacids, and products ... Fluoroquinolones can enter cells easily via porins and, therefore, are often used to treat intracellular pathogens such as ... Fluoroquinolones have varying specificity for cytochrome P450, and so may have interactions with drugs cleared by those enzymes ... The mechanisms of the toxicity of fluoroquinolones have been attributed to their interactions with different receptor complexes ...
This complex interaction can include noncovalent interactions such as van der Waals, electrostatic and hydrogen bonds. ... such as gram-negative porins, which are pore-forming proteins. The inner, plasma membrane is also generally symmetric whereas ... Hydrophobic interactions (also known as the hydrophobic effect) are the major driving forces in the formation of lipid bilayers ... An increase in interactions between hydrophobic molecules (causing clustering of hydrophobic regions) allows water molecules to ...
In a few cases, those of Kir1.1a, Kir6.1 and Kir6.2, for example, direct interaction with a member of the ABC superfamily has ...
... are molecules so small that they can permeate the mitochondrial outer membrane through porins and shuttle between two ...
October 1994). "Porins from plants. Molecular cloning and functional characterization of two new members of the porin family". ... Interaction of internal components of vesicles may have led to formation of the endoplasmic reticulum and contributed to the ... Transport proteins called porins are found in the outer membranes of mitochondria and chloroplasts and are also found in ... that the transport proteins called porins are found in the outer membranes of mitochondria, chloroplasts and bacterial cell ...
Approximately four hundred mutations of the GJB1 gene have been identified in people with X-linked Charcot-Marie-Tooth disease (CMTX).[14] CMTX is predominantly classified with symptoms related to muscle weakness and sensory problems, especially in the outer extremities of the limbs.[8] CMTX is the second most common type of CMT (about 10% of all patients) and is transmitted as an x-linked dominant trait.[7] It is categorised by the lack of male-to-male transmission of the mutated GJB1 gene and the differences in severity between heterozygous women and hemizygous men, with the later being more severely affected.[11]. Most of the mutations of the GJB1 gene switch or change a single amino acid in the gap junction (connexin-32) protein, although some may result in a protein of irregular size.[7][11][13][14] Some of these mutations also cause hearing loss in patients with CMTX.[14] Currently it is unknown how the mutations of the GJB1 gene lead to these specific features of Charcot-Marie-Tooth ...
Molecular cloning and functional characterization of two new members of the porin family". J Biol Chem. 269 (41): 25754-25760. ... Interaction of internal components of vesicles may have led to formation of the endoplasmic reticulum and contributed to the ... Zeth K.; Thein, M. (2010). "Porins in prokaryotes and eukaryotes: common themes and variations". Biochem J. 431 (1): 13-22. doi ... Fischer, K, Weber, A, Brink, S, Arbinger, B, Schünemann, D, Borchert, S, Heldt, HW, Popp, B, Benz, R, Link, TA (1994). "Porins ...
Piscitelli, Stephen C.; Keith Rodvold (2005). Drug Interactions in Infectious Diseases. Humana Press. ISBN 978-1-58829-455-5. . ... They are short-acting and passively diffuse through porin channels in the bacterial membrane. They inhibit protein synthesis by ... Archer JS, Archer DF (June 2002). "Oral contraceptive efficacy and antibiotic interaction: a myth debunked". Journal of the ...
General bacterial porin family, known as trimeric porins (n=16,S=20) ... Outer membrane protein G porin family (n=14,S=16). Note: n and S are, respectively, the number of beta-strands and the "shear ... MspA porin (octamer, n=S=16) and α-hemolysin (heptamer n=S=14) . These proteins are secreted. ...
The molecular interactions are apparent upon performing a detailed analysis of the structures of PA, EF, LF, and the cellular ... which is a pattern conserved in the membrane-spanning portions of porins. The only problem is that the loop is not large enough ... This interaction demonstrates less freedom to unfold the Greek key. Further analysis shows that seven of the nine histidines in ... The co-crystal structure of the PA octamer in complex with N-terminal LF revealed that the binding interaction is, in fact, two ...
... was extensively studied in Escherichia coli. E. coli grows faster on glucose than on any other carbon source. For example, if E. coli is placed on an agar plate containing only glucose and lactose, the bacteria will use glucose first and lactose second. When glucose is available in the environment, the synthesis of β-galactosidase is under repression due to the effect of catabolite repression caused by glucose. The catabolite repression in this case is achieved through the utilization of phosphotransferase system. An important enzyme from the phosphotransferase system called Enzyme II A (EIIA) plays a central role in this mechanism. There are different catabolite-specific EIIA in a single cell, even though different bacterial groups have specificities to different sets of catabolites. In enteric bacteria one of the EIIA enzymes in their set is specific for glucose transport only. When glucose levels are high inside the bacteria, EIIA mostly exists in its unphosphorylated ...
... the Alpha Interaction Domain, AID) and a region on the GK domain of the β subunit (Alpha Interaction Domain Binding Pocket) was ... Until very recently, the interaction between a highly conserved 18-amino acid region on the α1 subunit intracellular linker ... opening the possibility of the β subunit having multiple regulatory interactions with the α1 subunit pore. Furthermore, the AID ...
... is a form of albinism which, in contrast to oculocutaneous albinism, presents primarily in the eyes.[1] There are multiple forms of ocular albinism, which are clinically similar.[2]:865. Both known genes are on the X chromosome. When the term "autosomal recessive ocular albinism" ("AROA") is used, it usually refers to mild variants of oculocutaneous albinism rather than ocular albinism, which is X-linked.[3]. ...
Voltage-dependent calcium channel gamma-4 subunit is a protein that in humans is encoded by the CACNG4 gene.[5][6] L-type calcium channels are composed of five subunits. The protein encoded by this gene represents one of these subunits, gamma, and is one of several gamma subunit proteins. It is an integral membrane protein that is thought to stabilize the calcium channel in an inactive (closed) state. This gene is a member of the neuronal calcium channel gamma subunit gene subfamily of the PMP-22/EMP/MP20 family and is located in a cluster with two similar gamma subunit-encoding genes.[6] ...
The muscle weakness and increased risk of irregular heart beat in TPP result from markedly reduced levels of potassium in the bloodstream. Potassium is not in fact lost from the body, but increased Na+/K+-ATPase activity (the enzyme that moves potassium into cells and keeps sodium in the blood) leads to shift of potassium into tissues, and depletes the circulation. In other types of potassium derangement, the acid-base balance is usually disturbed, with metabolic alkalosis and metabolic acidosis often being present. In TPP, these disturbances are generally absent. Hypokalemia leads to hyperpolarization of muscle cells, making the neuromuscular junction less responsive to normal nerve impulses and leading to decreased contractility of the muscles.[1] It is not clear how the described genetic defects increase the Na+/K+-ATPase activity, but it is suspected that the enzyme becomes more active due to increased thyroid hormone levels. Hyperthyroidism increases the levels of catecholamines (such as ...
Interactions[edit]. TRPC5 has been shown to interact with STMN3,[12] TRPC1,[13][14] and TRPC4.[14] ... "Formation of novel TRPC channels by complex subunit interactions in embryonic brain". J. Biol. Chem. 278 (40): 39014-9. doi: ...
1.1 Porin. 2 Intermembrane space. 2.1 Intracristal space. 2.2 Peripheral space. 3 Lamella. ...
Porins usually arrange themselves in membranes as trimers. See also[edit]. *Oligomer ...
Porins exist in the outer membrane, which act like pores for particular molecules ...
The flow of potassium ions is interrupted by interaction of an inactivating particle, an auxiliary protein in humans but an ... The I to V amino acid change is thought to disrupt the hydrophobic interaction between the inactivating particle and the pore ...
Voltage-dependent calcium channel subunit alpha2delta-2 is a protein that in humans is encoded by the CACNA2D2 gene.[5] This gene encodes a member of the alpha-2/delta subunit family, a protein in the voltage-dependent calcium channel complex. Calcium channels mediate the influx of calcium ions into the cell upon membrane polarization and consist of a complex of alpha-1, alpha-2/delta, beta, and gamma subunits in a 1:1:1:1 ratio. Various versions of each of these subunits exist, either expressed from similar genes or the result of alternative splicing. Research on a highly similar protein in rabbit suggests the protein described in this record is cleaved into alpha-2 and delta subunits. Alternate transcriptional splice variants of this gene, encoding different isoforms, have been characterized.[5] CACNA2D2 containing channels are blocked by amiodarone.[6] ...
Interaction of viral pathogen with porin channels on the outer membrane of insect bacterial symbionts mediates their joint ... Supplementary material from Interaction of viral pathogen with porin channels on the outer membrane of insect bacterial ... Supplementary material from Interaction of viral pathogen with porin channels on the outer membrane of insect bacterial ... 2018): Supplementary material from "Interaction of viral pathogen with porin channels on the outer membrane of insect bacterial ...
... human recombinant porin, or purified bovine porin). Increased ionic strength reduced the binding of MtCK to porin, suggesting ... This interaction was independent of the immobilized binding partner (porin reconstituted in liposomes or MtCK) or the analyzed ... A specific, direct protein-protein interaction between MtCK and mitochondrial porin was demonstrated using surface plasmon ... No interaction of MtCK with reconstituted adenine nucleotide translocator was detectable in our experimental setup. The ...
... help/interaction_section target=_top>More...,/a>,/p>Interactioni. Protein-protein interaction databases. Protein interaction ... porin activity Source: UniProtKB-KW. *voltage-gated anion channel activity Source: GO_Central ,p>Inferred from Biological ... p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or ... "Biochemical, molecular, and functional characterization of porin isoforms from potato mitochondria.". Heins L., Mentzel H., ...
Structural and functional characterization of OmpF porin mutants selected for larger pore size. I. Crystallographic analysis. ... Green solid line show hydrophobic interactions and green dashed lines show π-π and π-cation interactions. ... MATRIX PORIN OUTER MEMBRANE PROTEIN F A 340 Escherichia coli Mutation: D113G Gene Name(s): ompF cmlB coa cry tolF b0929 JW0912 ... Interactions are determined by geometric criteria as described in K. Stierand, M. Rarey (2010), Drawing the PDB: Protein-ligand ...
... characteristics of ligand-bound channels is utilised to characterise the binding site and molecular interactions in detail, ... results provide a rationale for the determinants that govern the binding and permeation of zwitterionic antibiotics in porin ... and atomistic biomolecular simulations reveals weak binding events between the β-lactam antibiotic ampicillin and the porin ... The permeation of most antibiotics through the outer membrane of Gram-negative bacteria occurs through porin channels. To ...
However, the regulation of porin genes and its own gene by CRP remains unclear in Y. pestis. Y. pestis employs a distinct ... Data presented here indicate a remarkable remodeling of the CRP-mediated regulation of porin genes and of its own one between ... to regulate porin genes independently. Although the CRP of Y. pestis shows a very high homology to that of E. coli, and the ... it controls the production of porins indirectly through its direct action on ompR-envZ. Auto-regulation of CRP has also been ...
Lipid interactions of alpha-helical protein toxins. In: Tamm, L.K, ed. Protein-Lipid Interactions: From Membrane Domains to ... Gram-negative trimeric porins have specific LPS binding sites that are essential for porin biogenesis. Proceedings of the ... Bacterial porin channels and voltage dependence. Experiments with two Brucella porins, one missing a 36 amino acid segment. ... Bacterial porin channels and voltage dependence experiments with two Brucella abortus porins, one missing a 36 amino acid ...
Microbe-host interactions: structure and role of gram-negative bacterial porins. Curr Protein Pept Sci. 2012;13:843-54. ... intracellular access to NH3 is provided by both diffusion through lipid bilayers and through porins. Porins are conductive, ... Im W, Roux B. Ions and counterions in a biological channel: a molecular dynamics simulation of OmpF porin from Escherichia coli ... Im W, Roux B. Ion permeation and selectivity of OmpF porin: a theoretical study based on molecular dynamics, brownian dynamics ...
Protein-lipid interactions prevent any water from passing along the outer channel wall. ... Enhanced water permeability and tunable ion selectivity in subnanometer carbon nanotube porins. Science 357, 792-796 (2017). ... Comment on "Enhanced water permeability and tunable ion selectivity in subnanometer carbon nanotube porins" ... Comment on "Enhanced water permeability and tunable ion selectivity in subnanometer carbon nanotube porins" ...
Meyer TF, Dehio C, Gray-owen SD (1998). "The role of neisserial Opa proteins in interactions with host cells". Trends Microbiol ... Opacity family porins are a family of porins from pathogenic Neisseria. These bacteria possess a repertoire of phase-variable ... opacity proteins that mediate various pathogen/host cell interactions. These proteins are related to OmpA-like transmembrane ...
"Microbe-host interactions: structure and role of Gram-negative bacterial porins". Current Protein & Peptide Science. 13 (8): ... The Omp50 Porin (Omp50 Porin) Family 1.B.61 - The Delta-Proteobacterial Porin (Delta-Porin) Family 1.B.62 - The Putative ... The majority of porins are monomers; however, some dimeric porins have been discovered, as well as an octameric porin. ... 1.B.1 - The General bacterial porin family 1.B.2 - The Chlamydial Porin (CP) Family 1.B.3 - The Sugar porin (SP) Family 1.B.4 ...
Interaction of non-classical detergents with mitochondrial porin. A new purification procedure and characterization of the pore ... Interaction of myelin basic protein with micelles of dodecylphosphocholine. Biochemistry 23: 6041-6046.PubMedCrossRefGoogle ... Interaction of the myelin basic protein with the anionic detergent sodium dodecyl sulfate. Biochem. J. 169: 281-285.PubMed ... Interactions between bovine myelin basic protein and zwitterionic lysophospholipids. Biochemistry. 29: 1142-1146.PubMedCrossRef ...
Porins / genetics * Protein Binding * Protein Folding * Protein Interaction Mapping * Protein Transport / physiology ...
Interaction of OmpR, a positive regulator, with the osmoregulated ompC and ompF genes of Escherichia coli. Studies with wild- ... Porin osmoregulation in cpxRA+ and cpxRA strains. The strains used were MDG147 (wild type) and EPB62 (cpxR::spc). Porin ... 3, porin expression in the cpxA cpxRD51A strain was comparable to porin expression in the wild-type strain. ... Our results indicate that porin regulation by the CpxA-CpxR system and by the EnvZ-OmpR system converges at the porin promoters ...
Regulatory interactions of Csr components: the RNA binding protein CsrA activates csrB transcription in Escherichia coli. J. ... In PA-2 lysogens, ipeX is transcribed in cis with the phage porin gene.The ipeX sequence located downstream of the nmpC porin ... we have shown that ipeX is synthesized from the phages porin gene promoter as part of the phage porin transcript at the 3′ ... One such alteration in the OmpC porin was an R74C substitution (OmpCR74C, or OmpC1Cys). This mutant porin and its derivative, ...
The profound stability of porin trimers is a product of tight monomer interactions. The monomer interface contains the amino- ... The E. coli Porin Proteins OMPF and Maltoporin (LamB Porin) Kristin Flammer 99, Aaron Downs 00, and David Marcey. Contents: I ... Porin Structure and Function: The General Diffusion Pores Matrix Porin III. Maltoporin Monomer Structure IV. Structural and ... Introduction to Porin Structure and Function: The General Diffusion Pores Matrix Porin (OMPF) Although there is little to no ...
Orientation and interactions of dipolar molecules during transport through OmpF porin. FEBS Letters 528(1-3): 53-57. ,http:// ... Figure 7. OmpF porin trimer. The looping strands of each porin protein bind with the adjacent loop of the next porin (Shown in ... OmpF Porin Regulation OmpF porin functions to regulate osmotic pressure between the cell and its surroundings. Many factors ... OmpF Porin Structure. --Primary Structure The OmpF porin gene contains 1807 nucleotide bases. 1086 of these 1807 nucleotides ...
protein domain specific binding Source: CAFA ,p>Inferred from Physical Interaction,/p> ,p>Covers physical interactions between ... "The TolB protein interacts with the porins of Escherichia coli.". Rigal A., Bouveret E., Lloubes R., Lazdunski C., Benedetti H. ... Protein-protein interaction databases. The Biological General Repository for Interaction Datasets (BioGRID) ... interactions target=_top>More...,/a>,/p>Binary interactionsi. Show more detailsHide details. P0A855. With. #Exp.. IntAct. ...
"Porins from marine bacteria of the genus Pseudoalteromonas (Gammaproteobacteria: Pseudoalteromonadaceae), Russian Journal of ... Cation Interactions and Biochemical Composition of the Cell Envelope of a Marine Bacterium ... Porins from marine bacteria of the genus Pseudoalteromonas (Gammaproteobacteria: Pseudoalteromonadaceae). Porins from marine ... Porins from marine bacteria of the genus Pseudoalteromonas (Gammaproteobacteria: Pseudoalteromonadaceae). Novikova, O.; ...
Search for predicted protein-protein interactions using: Search term: PA14_02370 Search term: porin ...
Drug Interactions. Presently, limited data are available on drug interactions involving eravacycline. Therefore, this section ... Tetracyclines generally enter Gram-negative bacterial cells through outer membrane porins OmpF and OmpC [1]. It is speculated ... Drug Interaction Facts. The authority on drug interactions. St. Louis: Lippincott Williams & Wilkins; 2012. p. 2011. ... Christ D, Sutcliffe J. TP-434 is metabolicallly stable and has low potential for drug-drug interactions [abstract no. F1-2162 ...
Search for predicted protein-protein interactions using: Search term: PA0189 Search term: probable porin ...
A) The interaction between Nup2p and Nup60p, and the effect of Kap60p. GST-Nup60p ( ... Gsp1p-GTP and Kap60p modulate the interaction between Nup60p and Nup2p. ( ... fig3: Gsp1p-GTP and Kap60p modulate the interaction between Nup60p and Nup2p. (A) The interaction between Nup2p and Nup60p, and ... fig3: Gsp1p-GTP and Kap60p modulate the interaction between Nup60p and Nup2p. (A) The interaction between Nup2p and Nup60p, and ...
One single lysine residue is responsible for the special interaction between polyphosphate and the outer membrane porin PhoE of ... The second porin-mediated mechanism, as described in this study, utilizes structural alterations in porin channels that ... The latter mechanism is utilized by N. gonorrhoeae because it expresses only a single porin allele, and gonococci lacking porin ... aerogenes porin conferred reduced susceptibility to cephalosporins (10). In vitro studies with E. coli porins have shown that ...
Molecular Characterization of the Interaction between Porins of Neisseria gonorrhoeae and C4b-Binding Protein Hanna Jarva, ... Interaction of TNF with TNF Receptor Type 2 Promotes Expansion and Function of Mouse CD4+CD25+ T Regulatory Cells Xin Chen, ... Apis Mellifera Venom and Melittin Block neither NF-κB-p50-DNA Interactions nor the Activation of NF-κB, Instead They Activate ...
Interaction between complement subcomponent C1q and the Klebsiella pneumoniae porin OmpK36. Infect Immun (1996) 64:4719-25. ... MHC/TCR interaction between APC and T cell decrease CD46 expression on T cell and allows cis interaction between jagged1 and ... Jagged1 binds Notch-1 and this interaction is responsible for cellular activation and proliferation. The cis interaction (on ... Interaction of the globular domain of human C1q with Salmonella typhimurium lipopolysaccharide. Biochim Biophys Acta (2008) ...
2010) Quantitative interaction proteomics and genome-wide profiling of epigenetic histone marks and their readers. Cell 142, ... Mouse-anti-porin antibodies (1:10,000) were purchased from Molecular Probes. Rabbit-anti-COPI coatomer antibodies (1:1,000) ... Identification of Transient Interaction Partners of the Membrane Proteins Pex30p and Pex29p. Our initial AP-AM strategy applied ... 1994) Coatomer interaction with di-lysine endoplasmic reticulum retention motifs. Science 263, 1629-1631. ...
To further understand how the interaction of ToxR with different promoters results in its function as an activator or repressor ... Sequence analysis revealed that ompT encodes a predicted 35.8 kDa outer membrane porin of V. cholerae. Primer extension ... we cloned ompT and characterized the ompT promoter and its interaction with ToxR. ...
Biotechnology Systems Aspects of Supported Membrane Biosensors; Biosensors from interactions of DNA with lipid membranes IV. ... Ion Selectivity, Specificity, and Membrane Reconstitution Investigation of substrate-specific porin channels in lipid bilayer ...
Modeled interactions between cells and stuff going on inside of cells. Delivery of particles, interactions, etc. also included ... Probably need 1 porin for each metal. Biosurfactants: part of crude oil refinery. Changes the hydrophobicity of the area ... GC has 3 H bonds holding it together, AT has 2 H bonds (so AT is a weaker binding than GC). Want the tightest interaction of ... Surfactant: absorb at surfaces by modifying chemical interactions. Basically detergents, ie: soap. Emulsifier: makes oil and ...
  • These bacteria possess a repertoire of phase-variable opacity proteins that mediate various pathogen/host cell interactions. (wikipedia.org)
  • Porins are beta barrel proteins that cross a cellular membrane and act as a pore, through which molecules can diffuse. (wikipedia.org)
  • Unlike other membrane transport proteins, porins are large enough to allow passive diffusion, i.e., they act as channels that are specific to different types of molecules. (wikipedia.org)
  • We are interested in the interactions and conformational changes of proteins in solution and in membranes . (ncl.ac.uk)
  • A special class of Escherichia coli outer membrane proteins (OMPs) called porins forms water-filled channels through which hydrophilic solutes gain access into the bacterial cell ( 27 ). (asm.org)
  • Porins have led the way in providing insights on the structure, regulation, and assembly of membrane proteins. (asm.org)
  • The OmpC and OmpF porin genes are transcriptionally regulated by a classical two-component signal transduction regulatory system consisting of the OmpR and EnvZ proteins ( 12 , 13 ). (asm.org)
  • The porin superfamily contains a number of homotrimeric (transmembrane) proteins that form water-filled pores across the outer cell membranes of gram-negative bacteria. (kenyon.edu)
  • Many factors affect OmpF porin regulation, however, one of the more known is through the proteins EnvZ and OmpR. (davidson.edu)
  • 4. The resulting OmpF mRNA undergoes translation and OmpF porin proteins are assembled and inserted into the outer membrane to prevent further osmotic pressure loss (Liu et al. (davidson.edu)
  • Using sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and the bilayer lipid membrane (BLM) technique, heat-modifiable porin-like proteins were identified among the OM proteins of marine pseudomonads. (deepdyve.com)
  • The nature of current fluctuations in the BLM and the conductivity of pores formed by these proteins suggest that these isolated porins are not identical in their functional properties. (deepdyve.com)
  • Selective permeability is ensured by membrane proteins, such as porins, which are attractive pharmacological targets in host pathogen interactions. (ipbs.fr)
  • Instead, such mutations consistently reduce or eliminate the function of transport proteins or porins, protein binding affinities, enzyme activities, the proton motive force, or regulatory control systems. (trueorigin.org)
  • As bacterial genomes typically encode multiple two-component proteins for distinct signalling pathways, the sequence and structural similarities of RR receiver domains create significant challenges to maintain interaction specificity. (pubmedcentralcanada.ca)
  • Conversely, as the sequence and structural similarity of the conserved core domains may be advantageous for interactions and communications between different signalling pathways, it also creates significant challenges to maintain the signalling specificity and fidelity among large numbers of similar signalling proteins within the same family. (pubmedcentralcanada.ca)
  • Thus, it becomes increasingly important to understand the interactions and specificity within a single family, which could lead to insights regarding the evolution of structure-function relationships of these signalling proteins as well as the discovery of novel cross-regulation between different pathways. (pubmedcentralcanada.ca)
  • Which of the following statements correctly distinguishes transporters (carrier proteins) from porins and ion channels? (studyblue.com)
  • Bacterial invasion was observed only for strains carrying the P.IA subtype of porin, which is typically associated with the development of disseminated neisserial disease, and did not require opacity outer membrane proteins, previously recognized as gonococcal invasins. (rupress.org)
  • Voltage-dependent anion channels (VDACs) are a family of three mitochondrial porins and the most abundant integral membrane proteins of the mitochondrial outer membrane (MOM). (rsc.org)
  • As proteins associated with the MOM, VDAC opening/closure as a metabolic checkpoint is regulated by protein-protein interactions, and is of pharmacological interest in pathological conditions such as cancer. (rsc.org)
  • RmpM is a putative peptidoglycan binding protein from Neisseria meningitidis that has been shown to interact with integral outer membrane proteins such as porins and TonB-dependent transporters. (rcsb.org)
  • We describe a method to pull membrane nanotubes from lipid vesicles to study the interaction of proteins or any curvature-active molecule with curved membranes in vitro . (jove.com)
  • D. radiodurans proteins were chosen based on STRING interactions with SlpA and Hpi and pertinent literature (see Figures 3 and 4, and text for details). (intechopen.com)
  • In this study, we identify the ICP2 proteins that mediate interactions. (usda.gov)
  • Several non-adhesin functional classes of proteins involved in host-pathogen interactions and pathogenesis are known to provide protection against bacterial infections. (biomedcentral.com)
  • Abstract Lipid protein interactions play a key role in the stability and function of various membrane proteins. (uni-konstanz.de)
  • The two major porins of Escherichia coli , namely OmpF and OmpC, form non-specific transport channels and allow for the passive diffusion of small, polar molecules (such as water, ions, amino acids, and other nutrients, as well as waste products) across the cell membrane. (biomedcentral.com)
  • We performed transposon mutagenesis of a two-color fluorescent reporter strain to identify new regulators of the porin genes ompF and ompC in Escherichia coli . (asm.org)
  • The classical porins OmpF and OmpC are major constituents of the Escherichia coli outer membrane and account for approximately 2% of the total protein content of the cell ( 52 ). (asm.org)
  • A novel mechanism of Escherichia coli porin regulation was discovered from multicopy suppressors that permitted growth of cells expressing a mutant OmpC protein in the absence of DegP. (asm.org)
  • Stimulation of the potassium sensor KdpD kinase activity by interaction with the phosphotransferase protein IIA(Ntr) in Escherichia coli. (labome.org)
  • Porins are water-filled pores and channels found in the membranes of bacteria and eukaryotes. (wikipedia.org)
  • Gram-positive bacteria lack outer membranes, so these porin channels are instead bound to specific lipids within the cell walls. (wikipedia.org)
  • Porins are also found in eukaryotes, specifically in the outer membranes of mitochondria and chloroplasts. (wikipedia.org)
  • 1-4 Thus IAM surfaces are intended to mimic the lipid environment of cell membranes and consequently the initial applications of IAM particles relate to endogenous solute-membrane interactions. (springer.com)
  • Biosensors from interactions of DNA with lipid membranes IV. (elsevier.com)
  • Reconstitution experiments were performed on lipid bilayer membranes in the presence of purified mitochondrial porin from yeast and of detergent-solubilized mitochondrial outer membranes of a porin-free yeast mutant. (meta.org)
  • The addition of the porin resulted in a strong increase of the membrane conductance, which was caused by the formation of ion-permeable channels in the membranes. (meta.org)
  • The neisserial porin P.I is a GTP binding protein that forms a voltage-gated channel that translocates into mammalian cell membranes and modulates host cell signaling events. (rupress.org)
  • OMP porins are present in the outer membranes of Gram-negative bacteria, mitochondria and plastids. (tcdb.org)
  • The permeation of most antibiotics through the outer membrane of Gram-negative bacteria occurs through porin channels. (nature.com)
  • The decreased influx rates of antibiotics into the bacteria caused by altered antibiotic-channel interactions can be aggravated by the overexpression of efflux pumps, which in combination, often leads to highly resistant phenotypes 22 . (nature.com)
  • Data presented here indicate a remarkable remodeling of the CRP-mediated regulation of porin genes and of its own one between these two bacteria. (biomedcentral.com)
  • Porins have threshold sizes of transportable molecules that depend on the type of bacteria and porin. (wikipedia.org)
  • Porins were first discovered in gram-negative bacteria, but gram-positive bacteria with both types of porins have been found. (wikipedia.org)
  • They exhibit similar transport functions but have a more limited variety of porins, compared to the distribution found in gram-negative bacteria. (wikipedia.org)
  • Whereas volatile compounds constitute a large class of potential infochemicals, their role in bacteria-bacteria interactions remains vastly unexplored. (wiley.com)
  • However, volatile compounds have been studied primarily in a context of inter-kingdom responses and little is known about their potential roles in bacteria-to-bacteria interactions. (wiley.com)
  • Host-pathogen interactions have been examined with use of microarrays to determine the transcriptional profiles of Salmonella enterica infecting macrophages ( 4 ) and human epithelial cells infected with P. aeruginosa ( 12 ). (asm.org)
  • Better prediction accuracy of Jenner-Predict web server signifies that domains involved in host-pathogen interactions and pathogenesis are better criteria for prediction of PVCs. (biomedcentral.com)
  • VDAC porin. (tcdb.org)
  • Here, we review the known protein interactions for each VDAC isoform in order to shed light on their peculiar roles in physiological and pathological conditions. (rsc.org)
  • Nevertheless, the so far explored VDAC-protein interactions for each isoform show that VDAC1 is mainly involved in the maintenance of cellular homeostasis and in pro-apoptotic processes, whereas VDAC2 displays an anti-apoptotic role. (rsc.org)
  • VDAC1, also named as VDAC, porin 31HM, porin 31HL and plasmalemmal porin, belongs to the eukaryotic mitochondrial porin family. (ptglab.com)
  • Various lysates were subjected to SDS PAGE followed by western blot with 66345-1-Ig (VDAC1/Porin antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours. (ptglab.com)
  • Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 66345-1-Ig (VDAC1/Porin antibody) at dilution of 1:1000 (under 10x lens). (ptglab.com)
  • Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 66345-1-Ig (VDAC1/Porin antibody) at dilution of 1:1000 (under 40x lens). (ptglab.com)
  • Mitochondrial creatine kinase (MtCK) co-localizes with mitochondrial porin (voltage-dependent anion channel) and adenine nucleotide translocator in mitochondrial contact sites. (inserm.fr)
  • His research is focused on understanding the role of electrostatics in the structure-function properties of mnultiionic ion channels like several bacterial porins, the mitochondrial voltage dependent anion channel, coronavirus E protein channels and other channel forming peptides. (uji.es)
  • The over-expression of OmpX can balance the decreased expression of non-specific porins, OmpF and OmpC, for the exclusion of small harmful molecules. (biomedcentral.com)
  • However, at least for the classical porins OmpF and OmpC, our results show that the Cpx envelope stress response system plays a role in regulating their expression. (asm.org)
  • The two classical porins OmpC and OmpF consist of three 16-stranded β barrels, each of which forms a channel that is restricted in the middle due to the inward folding of a loop ( 8 ). (asm.org)
  • One such alteration in the OmpC porin was an R74C substitution (OmpC R74C , or OmpC 1Cys ). (asm.org)
  • This mutant porin and its derivative, OmpC R74C,G154C , or OmpC 2Cys ( 17 ), are the subjects of this work and are further discussed below. (asm.org)
  • Here, the lysogenization event leads to the expression of a phage-encoded porin, Lc, and the inhibition of the host's OmpC porin. (asm.org)
  • The work conducted in this study will shed light on how phage porin expression regulates that of ompC . (asm.org)
  • The alignment of E. coli OmpF (ECOOMPF), E. coli OmpC (ECOOMPC), E. coli PhoE (ECOPHOE), Haemophilus influenzae Rd P2 porin (HINP2), Neisseria meningitidis PorB (NMEPORB), Bordetella pertussis porin (BPEPOR), Comamonas acidovorans Omp32 (CACPOR), Rhodopseudomonas blastica porin (RBLPOR), and Rhodobacter capsulatus porin (RCAPOR) is basically that of Jeanteur et al. (asm.org)
  • No direct interaction between OmpC and HslT was detectable, possible explanations for this lack of interaction are discussed. (ubc.ca)
  • Here we show that a combination of high-resolution electrophysiology, new noise-filtering analysis protocols and atomistic biomolecular simulations reveals weak binding events between the β-lactam antibiotic ampicillin and the porin PorB from the pathogenic bacterium Neisseria meningitidis . (nature.com)
  • We set out to investigate the interaction between ampicillin and PorB from Neisseria meningitidis , the pathogen that causes bacterial meningitis. (nature.com)
  • Opacity family porins are a family of porins from pathogenic Neisseria. (wikipedia.org)
  • The neisserial porins are the major protein components of the outer membrane of the pathogenic Neisseria (N. meningitidis and N. gonorrhoeae). (rupress.org)
  • In this study, we investigate the interaction of PorB from Neisseria meningitidis with TLR2 and describe the direct binding of a bacterial protein to TLR2 for the first time. (jimmunol.org)
  • The specific amino acids in the channel determine the specificity of the porin to different molecules. (wikipedia.org)
  • Porins are primarily involved in passively transporting hydrophilic molecules of various sizes and charges across the membrane. (wikipedia.org)
  • A generally accepted view of the porin assembly pathway is that after the removal of the signal peptide from precursors, mature porin molecules transiently exist in the periplasm as soluble or peripherally membrane-associated, thermolabile intermediates. (asm.org)
  • OmpF porin is a non-specific transport channel that allows for the passive diffusion of small, polar molecules (600-700 Da in size) through the cell's outer membrane. (davidson.edu)
  • Neisserial porins induce B lymphocytes to express costimulatory B7-2 molecules and to proliferate. (rupress.org)
  • We are particularly interested in how highly specific knowledge of radiation interactions with isolated molecules can be exploited to enhance our understanding of processes in more complex environments, notably irradiated biological media. (open.ac.uk)
  • The primary focus of this thesis is a detailed characterization and interpretation of the sub-level structure of the ionic current blockades produced during the interaction between single-stranded DNA and RNA molecules and the Staphylococcus aureus alpha-Hemolysin protein nanopore. (washington.edu)
  • The OmpF porin is found in the trimer formation in E. Coli . (davidson.edu)
  • OmpF porin functions to regulate osmotic pressure between the cell and its surroundings. (davidson.edu)
  • The OmpF porin gene contains 1807 nucleotide bases. (davidson.edu)
  • OmpF porin contains 340 amino acid residues. (davidson.edu)
  • These 22 amino acids form a precursor sequence to the OmpF porin. (davidson.edu)
  • They are eventually cleaved off to form OmpF porin (Inokuchi et al, 1982). (davidson.edu)
  • This precursor form of OmpF porin contains 362 amino acids. (davidson.edu)
  • They are cleaved from the amino acid sequence as the precursor is being converted to OmpF porin (Inokuchi et al, 1982). (davidson.edu)
  • The amino acid sequence provides the basis for the formation of the b- barrel channel of OmpF porin. (davidson.edu)
  • Cartoon structures of OmpF porin have been deduced by x-ray crystallography. (davidson.edu)
  • OmpF porin from E. coli is the the pricipal path way for the β-lactam antibiotics. (jbsdonline.com)
  • The structure based sequence alignment shows that except Y 32 the constricting zone residues of OmpF porin involved in ampicilin binding (R 42, R 82, R 132, E 117, Y 32 and Y 22) are structurally aligned with the PhoE porin and OmpK36. (jbsdonline.com)
  • Molecular basis for the polyamine-OmpF porin interactions, an inhibitor and mutant studies (3) showed three residues (D 113, D 121 and Y 294) as putative sites of interaction between the channel and spermine. (jbsdonline.com)
  • The profound stability of porin trimers is a product of tight monomer interactions. (kenyon.edu)
  • Earlier we have reported the extreme thermal stability of porin from Paracoccus denitrificans reconstituted into liposomes. (uni-konstanz.de)
  • Our results show that a mixed lipid system (non-uniform bilayer) optimizes the thermal stability of porin as compared to the porin in pure lipids (uniform bilayer) or detergent micelles. (uni-konstanz.de)
  • These specificities are determined by the threshold sizes of the porins, and the amino acid residues lining them. (wikipedia.org)
  • We formulate an algorithm to predict the transmembrane beta strands in the family of bacterial porins based on the conformational parameters and surrounding hydrophobicities of amino acid residues. (semanticscholar.org)
  • However, the regulation of porin genes and its own gene by CRP remains unclear in Y. pestis . (biomedcentral.com)
  • Subsequent genetic analysis revealed that this phenotype is dependent on phosphorylation of the response regulator CpxR and that activation of CpxA in wild-type cells results in similar regulation of porin expression. (asm.org)
  • The amino acid composition of the porin β strands are unique in that polar and nonpolar residues alternate along them. (wikipedia.org)
  • It has been showed that ampicilin exhibits a strong interaction with the residues of the constriction zone of OmpF channel (2). (jbsdonline.com)
  • The alignment also suggest that the R. capsulatus , R. balastics may not posses this ampicilin binding property as none of the residues are conserved as in the case of E. coli porins. (jbsdonline.com)
  • The core regions of these porins have well conserved and structurally positioned positive and negatively charged residues on the opposite walls. (jbsdonline.com)
  • R. capsulatus porin and R. blastica porin in comparison with OmpF have no structurally conserved charged residues. (jbsdonline.com)
  • In all the sixteen-stranded porins except R. blastica , the positively charged residues are aligned on one side of the wall (between 0°-180°) and the negatively charged residues are on the opposite side of the wall (between 180°-360°) along the pore axis (Figure 1). (jbsdonline.com)
  • Figure 1: The charged residues that are lining the opposite walls of the pore for sixteen-stranded porins are shown. (jbsdonline.com)
  • These three residues that are protruding into the pore are conserved among the E. coli porins and OmpK36 but not in the R. blastica and R. capsulatus porins, which differ in their pore architecture from the E. coli porins. (jbsdonline.com)
  • In the case of specific porins most of the channel-lining residues are conserved between LamB and ScrY porins. (jbsdonline.com)
  • The distribution of positively and negatively charged residues are almost equal on both sides of the wall in all the specific porins. (jbsdonline.com)
  • Among the aromatic residues involved in substrate binding, W 74, Y 41, Y 6, W 420 are structurally conserved among the three specific porins. (jbsdonline.com)
  • However, whether or not OmpX functions as a porin to modulate the membrane permeability is still unclear. (biomedcentral.com)
  • Additionally, porins can regulate permeability and prevent lysis by limiting the entry of detergents into the cell. (wikipedia.org)
  • Here, we use the bacterial porin OmpF from E. coli as a model system to gain insight on the control of outer membrane permeability by bacterial porins through the modulation of their open state. (mdpi.com)
  • In E. coli , which produces trimeric, high-permeability porins, the presence of this fraction has no functional consequence. (asm.org)
  • However, in fluorescent pseudomonads, which lack the high-permeability porin, this fraction functions as the major nonspecific porin. (asm.org)
  • We applied this approach to investigate the interactions of porins from E.coli with single antibiotics, and this will potentially provide information on the permeability rates. (embs.org)
  • OMPF ( o uter m embrane p rotein f ) is among the most general E. coli porins and is, therefore, used as a reference point in discussing the structures of more specific porins such as maltoporin. (kenyon.edu)
  • The β-barrel contains 18 strands in this protein, in contrast to the 16 strands seen in the trimeric porins. (asm.org)
  • Maltoporin, also known as the LamB porin, is among the best studied examples of the substrate-specific porins. (kenyon.edu)
  • Synthetic peptide corresponding to Human VDAC1/ Porin aa 150-250. (abcam.com)
  • VDAC1 porin. (tcdb.org)
  • The interactions involving VDAC1 have been characterized more in depth than those involving VDAC2 and VDAC3. (rsc.org)
  • In pathological conditions, namely neurodegenerative and cardiovascular diseases, both VDAC1 and VDAC2 establish abnormal interactions aimed to counteract the mitochondrial dysfunction which contributes to end-organ damage. (rsc.org)
  • In particular, an asymmetry often seen in the electrophysiological characteristics of ligand-bound channels is utilised to characterise the binding site and molecular interactions in detail, based on the principles of electro-osmotic flow through the channel. (nature.com)
  • To understand the molecular mechanism by which ToxR represses OmpT expression, we cloned ompT and characterized the ompT promoter and its interaction with ToxR. (nih.gov)
  • Image: Molecular model of porin, ribbon diagram. (open.ac.uk)
  • 2003). Porin OmpP2 of Haemophilus influenzae shows specificity for nicotinamide-derived nucleotide substrates. (tcdb.org)
  • Some porins are cation-selective, others are anion-selective and still others are selective for specific compounds (e.g., sugars, nucleotides, phosphate, pyrophosphate). (tcdb.org)
  • It is likely that Y. pestis OmpR and CRP respectively sense different signals (medium osmolarity, and cellular cAMP levels) to regulate porin genes independently. (biomedcentral.com)
  • Gene regulatory networks (GRN) computationally represent interactions among regulatory genes and their targets. (scielo.br)
  • We obtained a network with a larger number of regulatory genes, target genes and interactions as compared to the previously reported network. (scielo.br)
  • Two types of porins exist to transport different materials- general and selective. (wikipedia.org)
  • Selective porins are smaller than general porins, and have specificities for chemical species. (wikipedia.org)
  • Some of these stools also contained ICP2-resistant isogenic V. cholerae strains harboring missense mutations in the trimeric outer membrane porin protein OmpU, identifying it as the ICP2 receptor. (usda.gov)
  • Hydrogen bonding and dipole-dipole interactions between each monomer in the homotrimer ensure that they do not dissociate, and remain together in the outer membrane. (wikipedia.org)
  • It has been possible to isolate a number porins in their active forms and generate monomer crystal structures. (kenyon.edu)
  • The monomer interface contains the amino-carboxy salt bridge and provides a hydrophobic core through extensive (mainly hydrophobic) residue interactions over 35% of the molecule. (kenyon.edu)
  • Loop 2, colored blue, plays a role in interaction of the monomer with its neighboring unit. (asm.org)
  • A specific, direct protein-protein interaction between MtCK and mitochondrial porin was demonstrated using surface plasmon resonance spectroscopy. (inserm.fr)
  • Increasing temperature increases the fluidity of a phospholipid bilayer by decreasing hydrophobic interactions. (coursehero.com)
  • Thus, yeast porins function in mitochondrial phospholipid metabolism, and porin-mediated regulation of CL metabolism appears to be evolutionarily conserved. (tcdb.org)
  • Mitochondrial creatine kinase and mitochondrial outer membrane porin show a direct interaction that is modulated by calcium. (inserm.fr)
  • ipeX was localized within an untranslated region of 247 base pairs between the stop codon of nmpC -a remnant porin gene from the cryptic phage qsr ′ (DLP12) genome-and its predicted Rho-independent transcriptional terminator. (asm.org)
  • Outer membrane porin F (gene oprF) from Pseudomonas. (ebi.ac.uk)
  • The outer membrane contains groups of three porins called trimers. (davidson.edu)
  • Porins are composed of beta sheets (β sheets) made up of beta strands (β strands) which are linked together by beta turns on the cytoplasmic side and long loops of amino acids on the other. (wikipedia.org)
  • The β barrels that make up a porin are composed of as few as eight β strands to as many as twenty-two β strands. (wikipedia.org)
  • Depending on the size of the porin, the interior of the protein may either be filled with water, have up to two β strands folded back into the interior, or contain a "stopper" segment composed of β strands. (wikipedia.org)
  • Strand number (n) is the amount of β strands in the porin, and barrel radius (R) refers to the radius of the opening of the porin. (wikipedia.org)
  • Identification of membrane spanning beta strands in bacterial porins. (semanticscholar.org)
  • Interestingly, another prophage, PA-2, which encodes a porin similar to NmpC, known as Lc, has sequences downstream from lc identical to that of ipeX . (asm.org)
  • Alterations in por IB , one of two alleles at the por locus that encodes the outer membrane protein porin IB (PIB), were recently reported to be responsible for the increased resistance to penicillin and tetracycline conferred by penB , but the specific mutations conferring antibiotic resistance were not identified experimentally. (asm.org)
  • Sequence analysis revealed that ompT encodes a predicted 35.8 kDa outer membrane porin of V. cholerae. (nih.gov)
  • Die Struktur enthält ein in der Porenengstelle gebundenes Anion, das eine Funktion des Porins als Anionen-spezifischen Kanal für kleine organische Säuren nahelegt. (tum.de)
  • B. Porins and ion channels have a permanent hole that passes from one side of the membrane to the other through which solutes pass whereas transporters do not. (studyblue.com)
  • Note that Nup2p binds Nup60p, that Kap60p prevents the interaction, and that the NH2 terminus of Nup2p is not required for binding Nup60p. (nih.gov)
  • To further understand how the interaction of ToxR with different promoters results in its function as an activator or repressor, we have also mapped the regions on the ctxAB and toxT promoters to which ToxR binds. (nih.gov)
  • A. .Transporters exhibit a conformational change when substrate binds but porins and ion channels do not. (studyblue.com)
  • Maltoporin is also one of the many porins which contains a bacteriophage recognition sequence. (kenyon.edu)
  • Although there is little to no sequence homology among porins, they often share a strong structural resemblance. (kenyon.edu)
  • Sequence alignment of porins from α-, β-, and γ-proteobacteria. (asm.org)
  • Sequence comparisons and three-dimensional structural analyses suggest that many of the families described under category 1.B are related (see porin superfamilies in TCDB and ( Reddy and Saier 2016 ). (tcdb.org)
  • This interaction was independent of the immobilized binding partner (porin reconstituted in liposomes or MtCK) or the analyzed isoform (chicken sarcomeric MtCK or human ubiquitous MtCK, human recombinant porin, or purified bovine porin). (inserm.fr)
  • These sequences are putative porins. (ebi.ac.uk)
  • A porin and two immunophilins were identified as putative interactors. (uni-koeln.de)
  • Green solid line show hydrophobic interactions and green dashed lines show π-π and π-cation interactions. (rcsb.org)
  • We pay attention not only to the effects of charges in the hydrophilic lipid heads but also to the contribution of the hydrophobic tails in the lipid-protein interactions. (mdpi.com)
  • Depletion of the porins Por1 and Por2 destabilized Ups1 and Ups2, decreased CL levels by ~90%, and caused loss of Ups2-dependent phosphatidylethanolamine synthesis, but did not affect Ups2-independent phosphatidylethanolamine synthesis in mitochondria. (tcdb.org)
  • General porins have no substrate specificities, though some exhibit slight preferences for anions or cations. (wikipedia.org)
  • Interaction of Pseudomonas aeruginosa with host cells in vitro has typically been studied using a variety of immortalized mammalian cell lines ( 1 , 7 , 23 ). (asm.org)
  • Dissemination in the mammalian host requires temporal regulation of virulence determinants to allow for vascular interactions, invasion, and colonization of distal tissues. (frontiersin.org)
  • Using HeLa cells, the authors showed that mammalian porins also function in mitochondrial CL metabolism. (tcdb.org)
  • The outer membrane contains a number of porins and transporters to facilitate the import of nutrients while simultaneously protecting cells from extracellular assault. (ubc.ca)
  • Interactions of MotX with MotY and with the PomA/PomB sodium ion channel complex of the Vibrio alginolyticus polar flagellum. (ebi.ac.uk)
  • A few porins display substrate-specificity in addition to general diffusion properties. (kenyon.edu)
  • Further application of the FRET method to all E. coli OmpR/PhoB subfamily RRs revealed that phosphorylation-activated RR interaction is indeed a common theme for OmpR/PhoB subfamily RRs and these RRs display significant interaction specificity. (pubmedcentralcanada.ca)
  • In vitro characterization of peptidoglycan-associated lipoprotein (PAL)-peptidoglycan and PAL-TolB interactions. (ebi.ac.uk)
  • Characterization of interactions for BtuB, Colicin. (ubc.ca)
  • Characterization of interactions for BtuB, Colicin E3, and HslTbyAllan MillsB.Sc. (ubc.ca)
  • 2011). Characterization of a porin channel in the endosymbiont of the trypanosomatid protozoan Crithidia deanei. (tcdb.org)
  • Here we describe the results of a genetic screen and a subsequent analysis which indicated that a second two-component system, the CpxA-CpxR system, also regulates porin expression. (asm.org)
  • PorA and PorH are the most important porins in Corynebacterium glutamicum. (ipbs.fr)
  • C. Porins and ion channels mediate passive transport, while transporters are associated with both passive and active transport. (studyblue.com)
  • This would promote more efficient peptidoglycan binding, by allowing RmpM to interact simultaneously with two glycan chains through its C-terminal, OmpA-like binding domain, while its (structurally uncharacterized) N-terminal domain could stabilize oligomers of porins and TonB-dependent transporters in the outer membrane. (rcsb.org)
  • To explore the basis of their potent adjuvant activity, the effect of the neisserial porins on T-B cell interactions and T cell costimulation was examined. (rupress.org)
  • Neisserial porins increased the surface expression of the costimulatory ligand B7-2 (CD86) but did not affect the expression of B7-1 (CD80). (rupress.org)
  • In addition, incubation with the neisserial porins increased the T lymphocyte costimulatory ability of B lymphocytes, which was inhibited by anti-B7-2 but not anti-B7-1 monoclonal antibodies. (rupress.org)
  • Upregulation of B7-2 on the surface of B lymphocytes may be the mechanism behind the immunopotentiating activity of neisserial porins. (rupress.org)
  • The PhD thesis presented describes structural studies of porin Omp32 from Comamonas acidovorans, P100 from Thermus thermophilus and HVDAC1 and HVDAC2 from Homo sapiens. (tum.de)
  • Protein-lipid interactions prevent any water from passing along the outer channel wall. (sciencemag.org)
  • Die Struktur des bakteriellen Porins Omp32 wurde durch Röntgenkristallografie über die SIRAS-Methode bei 2,1 Å gelöst. (tum.de)
  • Using a dual track SILAC-based quantitative interaction proteomics approach, we established a comprehensive network of stable as well as transient interactions of the peroxin Pex30p, an integral membrane protein. (mcponline.org)
  • It thus appears that two distinct two-component systems, CpxA-CpxR and EnvZ-OmpR, converge at the porin promoters. (asm.org)