Integrons
Drug Resistance, Multiple, Bacterial
Integrases
Drug Resistance, Bacterial
Microbial Sensitivity Tests
Conjugation, Genetic
Salmonella enterica
Sequence Analysis, DNA
DNA Transposable Elements
Polymerase Chain Reaction
Electrophoresis, Gel, Pulsed-Field
Plasmids
beta-Lactamases
Enterobacteriaceae
Gene Transfer, Horizontal
Acinetobacter baumannii
Drug Resistance, Microbial
Molecular Sequence Data
Gram-Negative Bacteria
Enterobacter cloacae
Dysentery, Bacillary
Delftia
Escherichia coli
Random Amplified Polymorphic DNA Technique
Interspersed Repetitive Sequences
Shigella
Acinetobacter
Attachment Sites, Microbiological
Gram-Negative Bacterial Infections
Klebsiella pneumoniae
Poultry
Salmonella
Bacteriophage Typing
DNA Fingerprinting
Environmental Microbiology
Trimethoprim
Base Sequence
Genomic Islands
Pseudomonas aeruginosa
Blotting, Southern
Drug Resistance, Multiple
Aminoglycosides
Occurrence of antibiotic resistance gene cassettes aac(6')-Ib, dfrA5, dfrA12, and ereA2 in class I integrons in non-O1, non-O139 Vibrio cholerae strains in India. (1/493)
Molecular mechanisms of multidrug resistance in Vibrio cholerae belonging to non-O1, non-O139 serogroups isolated during 1997 to 1998 in Calcutta, India, were investigated. Out of the 94 strains examined, 22 strains were found to have class I integrons. The gene cassettes identified were dfrA1, dfrA15, dfrA5, and dfrA12 for trimethoprim; aac(6')-Ib for amikacin and tobramycin; aadA1 and aadA2 for streptomycin and spectinomycin; and ereA2 for erythromycin resistance. To our knowledge, this is the first report of the presence of dfrA5, dfrA12, aac(6')-Ib, and ereA2 cassettes in class I integrons of V. cholerae. Forty-three of 94 strains also had plasmids, and out of these, 14 contained both class I integrons and plasmids. Pulsed-field gel electrophoresis followed by Southern hybridization revealed that in the 14 plasmid-bearing strains, class I integrons resided either on chromosomes, on plasmids, or on both. Our results indicated that besides class I integrons and plasmids, a conjugative transposon element, SXT, possibly contributed to the multiple antibiotic resistance. (+info)Characterization of a self-transferable plasmid from Salmonella enterica serotype typhimurium clinical isolates carrying two integron-borne gene cassettes together with virulence and drug resistance genes. (2/493)
An unusual self-transferable virulence-resistance plasmid (pUO-StVR2) was found in nine multidrug-resistant (ACSSuT phenotype) Salmonella enterica serotype Typhimurium clinical isolates that were assigned to four different phage types and a single and distinctive XbaI pulsed-field gel electrophoresis profile. pUO-StVR2 is an IncFII plasmid of about 140 kb in length carrying the spvA, spvB, and spvC (Salmonella plasmid virulence) and rck (resistance to complement killing) genes. It also carries the oxa1/aadA1a (ampicillin resistance and streptomycin-spectinomycin resistance) gene cassette configuration located within a class 1 integron with qacEDelta1/sul1 (ammonium antiseptics resistance and sulfadiazine resistance); the transposon genes merA, tnpA, and tnpR (mercury resistance, transposase, and resolvase of Tn21, respectively); and the catA1 (chloramphenicol resistance) and tet(B) (tetracycline resistance) genes. The insertion of resistance genes into a Salmonella virulence plasmid constitutes a new and interesting example of plasmid evolution and presents a serious public health problem. (+info)Low-virulence Citrobacter species encode resistance to multiple antimicrobials. (3/493)
Citrobacter spp. are gram-negative commensal bacteria that infrequently cause serious nosocomial infections in compromised hosts. They are often resistant to cephalosporins due to overexpression of their chromosomal beta-lactamase. During a recent study of multidrug-resistant Enterobacteriaceae (MDRE) in solid-organ transplant patients, we found that almost half of patients colonized with MDRE carried one or more cefpodoxime-resistant Citrobacter freundii, Citrobacter braakii, or Citrobacter amalonaticus strains. Pulsed-field gel electrophoresis showed that 36 unique strains of Citrobacter were present among 32 patients. Genetic and phenotypic analysis of the resistance mechanisms of these bacteria showed that the extended-spectrum beta-lactamase (ESBL) SHV-5 or SHV-12 was encoded by 8 strains (26%) and expressed by 7 strains (19%). A number of strains were resistant to other drug classes, including aminoglycosides (28%), trimethoprim-sulfamethoxazole (31%), and fluoroquinolones (8%). PCR and DNA analysis of these multiresistant strains revealed the presence of class I integrons, including the first integrons reported for C. braakii and C. amalonaticus. The integrons encoded aminoglycoside resistance, trimethoprim resistance, or both. Despite the prevalence of MDR Citrobacter spp. in our solid-organ transplant patients, only a single infection with a colonizing strain was recorded over 18 months. Low-virulence Citrobacter spp., which can persist in the host for long periods, could influence pathogen evolution by accumulation of genes encoding resistance to multiple antimicrobial classes. (+info)Class 1 integron-associated tobramycin-gentamicin resistance in Campylobacter jejuni isolated from the broiler chicken house environment. (4/493)
Using PCR, we screened 105 isolates of poultry-associated Campylobacter jejuni for the presence of class 1 integrons. Of those isolates, 21% (22 of 105) possessed the integrase gene, but only 5 isolates produced an amplicon in a 5'-3' conserved sequence PCR directed toward amplification of the resistance cassettes. DNA sequencing demonstrated that all five isolates possessed the aminoglycoside resistance gene, aacA4. (+info)Molecular characterization of integrons in epidemiologically unrelated clinical isolates of Acinetobacter baumannii from Italian hospitals reveals a limited diversity of gene cassette arrays. (5/493)
Integron carriage by 36 epidemiologically unrelated Acinetobacter baumannii isolates collected over an 11-year period from patients in six different Italian hospitals was investigated. Sixteen type 1 integron-positive isolates (44%) were found, 13 of which carried the same array of cassettes, i.e., aacC1, orfX, orfX', and aadA1a. As ribotype analysis of the isolates demonstrated a notable genetic diversity, horizontal transfer of the entire integron structure or ancient acquisition was hypothesized. (+info)Nosocomial infections caused by multidrug-resistant isolates of pseudomonas putida producing VIM-1 metallo-beta-lactamase. (6/493)
Successful carbapenem-based chemotherapy for the treatment of Pseudomonas infections has been seriously hindered by the recent appearance of IMP- and VIM-type metallo-beta-lactamases, which confer high-level resistance to carbapenems and most other beta-lactams. Recently, multidrug-resistant Pseudomonas putida isolates for which carbapenem MICs were >/=32 micro g/ml were recovered from cultures of urine from three inpatients in the general intensive care unit of the Ospedale di Circolo, Varese, Italy. Enzyme assays revealed production of a metallo-beta-lactamase activity, while molecular analysis detected in each isolate a bla(VIM-1) determinant carried by an apparently identical medium-sized plasmid. Conjugation experiments were unsuccessful in transferring the beta-lactamase determinant to Escherichia coli or Pseudomonas aeruginosa. Macrorestriction analysis by pulsed-field gel electrophoresis demonstrated that the isolates were of clonal origin. PCR mapping and sequencing of the variable region of the plasmid-borne class 1 integron carrying the bla(VIM-1) determinant (named In110) showed that the bla(VIM-1)-containing cassette was identical to that previously found in strains of different species from other Italian hospitals and that the cassette array of In110 was not identical but clearly related to that of In70 (a bla(VIM-1)-containing plasmid-borne integron from an Achromobacter xylosoxidans isolate), pointing to a common origin of this cassette and to a related evolutionary history of their cognate integrons. (+info)Tn5041-like transposons: molecular diversity, evolutionary relationships and distribution of distinct variants in environmental bacteria. (7/493)
A detailed study on the geographic distribution, molecular diversity and evolutionary relationships of 24 closely related variants of the Tn5041 transposon found among 182 mercury resistant environmental Gram-negative strains from the IMG-Hg Reference Collection is reported here. RFLP analysis, followed by the determination of partial DNA sequences, identified 14 distinct types of these transposons, which differed from each other by 1-7 single-event DNA polymorphisms. No polymorphisms were detected at the right arm of the transposons except an insertion of a new mobile DNA element carrying a mer operon (named the mer2 cassette) within the Tn5041 mer operon. According to the model presented here, the insertion occurred via homologous recombination with a circular form of the mer2 cassette. A total of 8 point mutations, 1 internal deletion, 2 end-involving deletions, 3 mosaic regions and 2 insertions were detected at the left arm of the transposons. The insertions were a transposon closely related to Tn21 but lacking the integron and a new group II intron (named INT5041C). Inspection of the geographic distribution of the Tn5041 variants suggested that at least three long-distance waves of dissemination of these variants had occurred, accompanied by homologous recombination between different Tn5041 lineages. Movements of circular DNAs by homologous recombination as a source of mosaic genes and new mer genes, and formation of unusual mosaics ending or beginning at the Tn5041 att site are discussed. (+info)In34, a complex In5 family class 1 integron containing orf513 and dfrA10. (8/493)
A complex class 1 integron, In34, found in a conjugative plasmid from a multidrug-resistant Klebsiella pneumoniae strain isolated in 1997 at a hospital in Sydney, Australia, was shown to have a backbone related to that of In2, which belongs to the In5 family. In In34, the aadB gene cassette replaces the aadA1a cassette in In2, and two additional resistance genes, dfrA10 and aphA1, that are not part of a gene cassette are present. The aphA1 gene is in a Tn4352-like transposon that is located in the tniA gene. The dfrA10 gene lies adjacent to a 2,154-bp DNA segment, known as the common region, that contains an open reading frame predicting a product of 513 amino acids (Orf513). Orf513 is 66 and 55% identical to the products of two further open reading frames that, like the common region, are found adjacent to antibiotic resistance genes. A 27-bp conserved sequence was found at one end of each type of common region. The loss of dfrA10 due to homologous recombination between flanking direct repeats and incorporation of the excised circle by homologous recombination were demonstrated. Part of In34 is identical to the sequenced portion of In7, which is from a multidrug-resistant Escherichia coli strain that had been isolated 19 years earlier in the same hospital. In34 and In7 are in plasmids that contain the same six resistance genes conferring resistance to ampicillin, chloramphenicol, gentamicin, kanamycin, neomycin, tobramycin, trimethoprim, and sulfonamides, but the plasmid backbones appear to be unrelated, suggesting that translocation of a multiple-drug-resistance-determining region as well as horizontal transfer may have occurred. (+info)Some common types of Acinetobacter infections include:
1. Pneumonia: This is an infection of the lungs that can cause fever, cough, chest pain, and difficulty breathing.
2. Urinary tract infections (UTIs): These are infections of the bladder, kidneys, or ureters that can cause symptoms such as burning during urination, frequent urination, and abdominal pain.
3. Bloodstream infections (sepsis): This is a serious and potentially life-threatening condition that occurs when bacteria enter the bloodstream and cause widespread inflammation. Symptoms can include fever, chills, rapid heart rate, and shortness of breath.
4. Skin and soft tissue infections: These are infections of the skin and underlying tissues that can cause redness, swelling, warmth, and pain.
5. Bacteremia: This is a condition in which bacteria enter the bloodstream and cause an infection.
6. Endocarditis: This is an infection of the heart valves, which can cause symptoms such as fever, fatigue, and shortness of breath.
Acinetobacter infections are often caused by the bacteria entering the body through a wound or surgical incision. They can also be spread through contact with contaminated surfaces or equipment in healthcare settings.
Treatment of Acinetobacter infections typically involves the use of antibiotics, which may be administered intravenously or orally. In some cases, surgical intervention may be necessary to remove infected tissue or repair damaged organs.
Prevention of Acinetobacter infections is important for reducing the risk of these infections occurring in healthcare settings. This can include proper hand hygiene, use of personal protective equipment (PPE), and effective cleaning and disinfection of surfaces and equipment.
Overall, Acinetobacter infections are a significant concern in healthcare settings, and prompt recognition and treatment are critical for preventing serious complications and improving patient outcomes.
Prevention of Salmonella Infections includes proper food handling and storage practices, such as cooking foods to the correct temperature, storing foods at the right refrigerator temperature, and washing hands frequently. Vaccines are also available for people who are at high risk of developing severe Salmonella infections.
Complications of a Salmonella Infection can include dehydration, bacteremia (the presence of bacteria in the bloodstream), and meningitis (inflammation of the lining around the brain and spinal cord). In rare cases, a Salmonella infection can lead to long-term health problems such as irritable bowel syndrome or reactive arthritis.
Overall, prompt treatment and proper prevention measures are important for reducing the risk of complications from a Salmonella infection.
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Here are some common types of E. coli infections:
1. Urinary tract infections (UTIs): E. coli is a leading cause of UTIs, which occur when bacteria enter the urinary tract and cause inflammation. Symptoms include frequent urination, burning during urination, and cloudy or strong-smelling urine.
2. Diarrheal infections: E. coli can cause diarrhea, abdominal cramps, and fever if consumed through contaminated food or water. In severe cases, this type of infection can lead to dehydration and even death, particularly in young children and the elderly.
3. Septicemia (bloodstream infections): If E. coli bacteria enter the bloodstream, they can cause septicemia, a life-threatening condition that requires immediate medical attention. Symptoms include fever, chills, rapid heart rate, and low blood pressure.
4. Meningitis: In rare cases, E. coli infections can spread to the meninges, the protective membranes covering the brain and spinal cord, causing meningitis. This is a serious condition that requires prompt treatment with antibiotics and supportive care.
5. Hemolytic-uremic syndrome (HUS): E. coli infections can sometimes cause HUS, a condition where the bacteria destroy red blood cells, leading to anemia, kidney failure, and other complications. HUS is most common in young children and can be fatal if not treated promptly.
Preventing E. coli infections primarily involves practicing good hygiene, such as washing hands regularly, especially after using the bathroom or before handling food. It's also essential to cook meat thoroughly, especially ground beef, to avoid cross-contamination with other foods. Avoiding unpasteurized dairy products and drinking contaminated water can also help prevent E. coli infections.
If you suspect an E. coli infection, seek medical attention immediately. Your healthcare provider may perform a urine test or a stool culture to confirm the diagnosis and determine the appropriate treatment. In mild cases, symptoms may resolve on their own within a few days, but antibiotics may be necessary for more severe infections. It's essential to stay hydrated and follow your healthcare provider's recommendations to ensure a full recovery.
The diagnosis of bacillary dysentery is based on a combination of clinical findings and laboratory tests, such as fecal cultures or polymerase chain reaction (PCR) assays. Treatment typically involves antibiotics, which can shorten the duration of diarrhea and reduce the risk of complications. In severe cases, hospitalization may be necessary to manage dehydration and other complications.
Prevention measures include maintaining good hygiene practices, such as washing hands after using the bathroom or before handling food, and avoiding contaminated water or food. Vaccines are also available for some types of Shigella infections.
Gram-negative bacterial infections can be difficult to treat because these bacteria are resistant to many antibiotics. In addition, some gram-negative bacteria produce enzymes called beta-lactamases, which break down the penicillin ring of many antibiotics, making them ineffective against the infection.
Some common types of gram-negative bacterial infections include:
* Pneumonia
* Urinary tract infections (UTIs)
* Bloodstream infections (sepsis)
* Meningitis
* Skin and soft tissue infections
* Respiratory infections, such as bronchitis and sinusitis
Examples of gram-negative bacteria that can cause infection include:
* Escherichia coli (E. coli)
* Klebsiella pneumoniae
* Pseudomonas aeruginosa
* Acinetobacter baumannii
* Proteus mirabilis
Gram-negative bacterial infections can be diagnosed through a variety of tests, including blood cultures, urine cultures, and tissue samples. Treatment typically involves the use of broad-spectrum antibiotics, such as carbapenems or cephalosporins, which are effective against many types of gram-negative bacteria. In some cases, the infection may require hospitalization and intensive care to manage complications such as sepsis or organ failure.
Prevention of gram-negative bacterial infections includes good hand hygiene, proper use of personal protective equipment (PPE), and appropriate use of antibiotics. In healthcare settings, infection control measures such as sterilization and disinfection of equipment, and isolation precautions for patients with known gram-negative bacterial infections can help prevent the spread of these infections.
Overall, gram-negative bacterial infections are a significant public health concern, and proper diagnosis and treatment are essential to prevent complications and reduce the risk of transmission.
Some common types of Serratia infections include:
1. Urinary tract infections (UTIs): Serratia bacteria can infect the urinary tract and cause symptoms such as burning during urination, frequent urination, and abdominal pain.
2. Skin infections: Serratia bacteria can cause skin infections, including cellulitis and abscesses, which can lead to redness, swelling, and pain in the affected area.
3. Respiratory tract infections: Serratia bacteria can infect the lungs and cause pneumonia, which can lead to symptoms such as coughing, fever, and difficulty breathing.
4. Bloodstream infections (sepsis): Serratia bacteria can enter the bloodstream and cause sepsis, a serious condition that can lead to organ failure and death if left untreated.
5. Endocarditis: Serratia bacteria can infect the heart valves and cause endocarditis, which can lead to symptoms such as fever, fatigue, and difficulty swallowing.
Serratia infections are typically diagnosed through a combination of physical examination, medical history, and laboratory tests, such as blood cultures and urinalysis. Treatment typically involves the use of antibiotics to eliminate the bacteria, and in severe cases, hospitalization may be necessary to monitor and treat the infection.
Preventive measures to reduce the risk of Serratia infections include practicing good hygiene, such as washing hands regularly, avoiding close contact with individuals who are sick, and maintaining proper cleanliness and sterilization practices in healthcare settings. Vaccines are not available for Serratia infections, but research is ongoing to develop new antimicrobial therapies and vaccines to combat antibiotic-resistant bacteria like Serratia.
The term "Salmonella Infections, Animal" is used to distinguish these infections from Salmonella infections that are caused by contaminated food or water, which are referred to as "Salmonella Infections, Human."
Klebsiella Infections can occur in anyone, but certain groups of people are at higher risk, such as premature infants, people with weakened immune systems, and those with chronic medical conditions like diabetes, liver or kidney disease.
Symptoms of Klebsiella Infections include fever, chills, cough, difficulty breathing, painful urination, redness and swelling in the affected area, and in severe cases, sepsis and death.
Diagnosis of Klebsiella Infections is typically made through a combination of physical examination, medical history, and laboratory tests, such as blood cultures and urine cultures.
Treatment of Klebsiella Infections usually involves antibiotics, which can help clear the infection and prevent it from spreading. In severe cases, hospitalization may be necessary to provide appropriate care and monitoring.
Prevention of Klebsiella Infections includes good hand hygiene, proper cleaning and disinfection of equipment and surfaces, and avoiding close contact with individuals who have the infection. Vaccines are also available for certain types of Klebsiella Infections, such as pneumonia.
Complications of Klebsiella Infections can include pneumonia, urinary tract infections, bloodstream infections, and sepsis, which can lead to organ failure and death if left untreated.
Recovery from Klebsiella Infections usually occurs within a few days to a week after antibiotic treatment is started, but in severe cases, recovery may take longer and may require hospitalization and close monitoring.
In conclusion, Klebsiella Infections are a type of bacterial infection that can affect various parts of the body, and can be mild or severe. Prompt diagnosis and treatment with antibiotics are essential to prevent complications and ensure a successful recovery. Proper hygiene practices and vaccines are also important for preventing the spread of these infections.
In medicine, cross-infection refers to the transmission of an infectious agent from one individual or source to another, often through direct contact or indirect exposure. This type of transmission can occur in various settings, such as hospitals, clinics, and long-term care facilities, where patients with compromised immune systems are more susceptible to infection.
Cross-infection can occur through a variety of means, including:
1. Person-to-person contact: Direct contact with an infected individual, such as touching, hugging, or shaking hands.
2. Contaminated surfaces and objects: Touching contaminated surfaces or objects that have been touched by an infected individual, such as doorknobs, furniture, or medical equipment.
3. Airborne transmission: Inhaling droplets or aerosolized particles that contain the infectious agent, such as during coughing or sneezing.
4. Contaminated food and water: Consuming food or drinks that have been handled by an infected individual or contaminated with the infectious agent.
5. Insect vectors: Mosquitoes, ticks, or other insects can transmit infections through their bites.
Cross-infection is a significant concern in healthcare settings, as it can lead to outbreaks of nosocomial infections (infections acquired in hospitals) and can spread rapidly among patients, healthcare workers, and visitors. To prevent cross-infection, healthcare providers use strict infection control measures, such as wearing personal protective equipment (PPE), thoroughly cleaning and disinfecting surfaces, and implementing isolation precautions for infected individuals.
In summary, cross-infection refers to the transmission of an infectious agent from one individual or source to another, often through direct contact or indirect exposure in healthcare settings. Preventing cross-infection is essential to maintaining a safe and healthy environment for patients, healthcare workers, and visitors.
Integron
Mobile genetic elements
Steve Negron
Gene cassette
KORE Wireless
Ruth Hall (scientist)
Plasmid-mediated resistance
SRNA-Xcc1
Repressor lexA
Ivan Erill
Beta-lactamase
Lacticaseibacillus casei
Morganella morganii
INTEGRALL
Integrase
Protein engineering
Transposable element
Acinetobacter
Acinetobacter baumannii
Alpha-D-phosphohexomutase superfamily
Carbapenem-resistant enterobacteriaceae
Christopher J. Schofield
Group II intron
Transposase
Human Microbiome Project
DUF1874 RNA motif
Shigella flexneri
Flaviflexus equikiangi
Enterobacter cloacae
Helitron (biology)
Integrons in Salmonella Keurmassar, Senegal - Volume 10, Number 7-July 2004 - Emerging Infectious Diseases journal - CDC
Notes from the Field: Domestically Acquired Verona Integron-Mediated Metallo-β-Lactamase-Producing Enterobacteriaceae - Indiana...
Integrons and gene cassettes in the enterobacteriaceae - PubMed
Class 1 and 2 Integrons in Escherichia coli Strains Isolated from Diarrhea and Bacteremia in Children Less Than 2 Years of Age...
Integron-mediated multidrug resistance in a global collection of nontyphoidal Salmonella enterica isolates - PubMed
Integron BelevingAwards2023 blokkenschema liggend - Integron 30 jaar Festival - BelevingAwards 2023
Genetic characterization of clinically relevant class 1 integrons carried by multidrug resistant bacteria (MDRB) isolated from...
Prevalence of class 1 integron in Escherichia coli isolated from animal sources in Iran: a systematic review and meta-analysis ...
Shigella sonnei genome sequencing and phylogenetic analysis indicate recent global dissemination from Europe | Nature Genetics
Mechanisms of Evolution in High-Consequence Drug Resistance Plasmids - PubMed
Publication Detail
Volume 14, Number 7-July 2008 - Emerging Infectious Diseases journal - CDC
Carl-Fredrik Flach | Göteborgs universitet
Biomarkers Search
One Health Genomic Analysis of Extended-Spectrum β-Lactamase‒Producing Salmonella enterica, Canada, 2012‒2016
Frontiers | Prevalence and molecular characteristics of polymyxin-resistant Enterobacterales in a Chinese tertiary teaching...
Towards safer vectors for the field release of recombinant bacteria | Environmental Biosafety Research
New carbapenem-resistant Enterobacteriaceae warrant additional action by healthcare providers
Effect of antibiotic prescribing in primary care on antimicrobial resistance in individual patients: systematic review and meta...
GtR
Carbapenem-Resistant Enterobacteriaceae: New Precautions
MeSH Browser
Ligand view of netilmicin (2604 - CIDUJQMULVCIBT-MQDUPKMGSA-N) - BRENDA Enzyme Database
WHO EMRO | Epidemiological characterization of P. aeruginosa isolates of intensive care units in Egypt and Saudi Arabia |...
Metagenomic analysis reveals the shared and distinct features of the soil resistome across tundra, temperate prairie, and...
SIGI: score-based identification of genomic islands | BMC Bioinformatics | Full Text
French National Research Agency | ANR
Novel Rapid Test for Detecting Carbapenemase
Comparative Genomics - MicroScope - Web Interface System & Specialized Databases
for (re)Annotation and Analysis of...
Genes7
- Integrons are assembly platforms - DNA elements that acquire open reading frames embedded in exogenous gene cassettes and convert them to functional genes by ensuring their correct expression. (nih.gov)
- Strains were screened for the integrons by polymerase chain reaction by using three sets of primers specific for the intI1, intI2, and intI3 genes coding for the integrase as described previously ( 3 ). (cdc.gov)
- Salmonella enterica bacteria have become increasingly resistant to antimicrobial agents, partly as a result of genes carried on integrons. (nih.gov)
- 7. Prevalence of dfr genes associated with integrons and dissemination of dfrA17 among urinary isolates of Escherichia coli in Korea. (nih.gov)
- 8. Multidrug resistance, prevalence and phylogenetic analysis of genes encoding class II and III integrons in clinically isolated Escherichia coli. (nih.gov)
- According to the importance of integrons in acquisition and dissemination of antibiotics resistance genes among these pathogens, so, the performance of antibiotic surveillance programs is recommended for control the spreading of antibiotics resistance genes. (longdom.org)
- In addition, long-read sequencing of one representative XDR ST235 isolate identified an integron carrying multiple resistance genes (including bla VIM-2), with differences in gene composition and synteny from the P. aeruginosa class 1 integrons described previously. (who.int)
Pseudomonas1
- The cassette aac (6′) -IIc was previously described in a single class 1 integron in Pseudomonas aeruginosa (AF162771). (cdc.gov)
Isolates8
- We evaluated the contribution of integrons to the antimicrobial drug resistance of eight isolates of S. enterica serovar Keurmassar sent to the Senegalese National Salmonella and Shigella Reference Laboratory at the Pasteur Institute in Dakar from March to May 2000. (cdc.gov)
- This cassette was since described in class 1 integrons of clinical gram-negative isolates and recently in a class 2 integron in Escherichia coli ( 9 ). (cdc.gov)
- We investigated this resistance and integron carriage among 90 isolates with the ACSSuT phenotype (resistance to ampicillin, chloramphenicol, streptomycin, sulfamethoxazole, and tetracycline) in a global collection of S. enterica isolates. (nih.gov)
- Four integrons, dfrA12/orfF/aadA2, dfrA1/aadA1, dfrA7, and arr2/blaOXA30/cmlA5/aadA2, were found in genetically unrelated isolates from 8 countries on 4 continents, which supports a role for horizontal gene transfer in the global dissemination of S. enterica multidrug resistance. (nih.gov)
- Serovar Typhimurium isolates containing identical integrons with the gene cassettes blaPSE1 and aadA2 were found in 4 countries on 3 continents, which supports the role of clonal expansion. (nih.gov)
- Additionally, we investigated the evolution and expansion of IS26 integrons in carbapenem -resistant K. pneumoniae isolates using long-read sequencing. (bvsalud.org)
- 9. Prevalence of Class 1 Integrons and Extended Spectrum Beta Lactamases among Multi-Drug Resistant Escherichia coli Isolates from North of Iran. (nih.gov)
- Our results showed relevance among class 1 and 2 integrons and MDR P. aeruginosa isolates. (longdom.org)
Antimicrobial drug resistance2
- Integrons are efficient gene-capture systems by site-specific recombination and are involved in antimicrobial-drug resistance in gram-negative bacteria ( 2 ). (cdc.gov)
- Clonal expansion and horizontal gene transfer may contribute to the spread of antimicrobial drug-resistance integrons in these organisms. (nih.gov)
Escherichia5
- 1. The Escherichia coli phylogenetic group B2 with integrons prevails in childhood recurrent urinary tract infections. (nih.gov)
- 3. Distribution of integron-associated trimethoprim-sulfamethoxazole resistance determinants among Escherichia coli from humans and food-producing animals. (nih.gov)
- 5. Integrons and antibiotic resistance in phylogenetic group B2 Escherichia coli. (nih.gov)
- 11. The presence of antibiotic resistance and integrons in Escherichia coli isolated from compost. (nih.gov)
- 14. Integron-mediated ESBL resistance in rare serotypes of Escherichia coli causing infections in an elderly population of Israel. (nih.gov)
Gene4
- The integron/gene cassette system: an active player in bacterial adaptation. (nih.gov)
- All these integrons, except that of serovar Infantis, contained a streptomycin-spectinomycin resistance determinant, aadA2 or mostly aadA1 , alone or in combination with other gene cassettes. (cdc.gov)
- Quantification of the mobile genetic element class 1 integron-integrase gene (intI1) has been proposed as a surrogate to measuring multiple ARGs. (nih.gov)
- PCR was carried out to detect the tox-A, class 1 and 2 integrons gene using the specific primers. (longdom.org)
Resistance determinants1
- To determine whether the resistance determinants carried by the integrons were transferable, we performed a conjugation experiment from S. enterica serovar Keurmassar to an E. coli strain resistant to nalidixic acid. (cdc.gov)
Antibiotic resistance1
- The role of integrons in the transfer of antibiotic resistance is one of the important issues, therefore, this study is aimed to investigate antibiotic resistance pattern and prevalence of class 1 and 2 integrons in P.aeruginosa isolated of nosocomial infection. (longdom.org)
Aeruginosa1
- RÉSUMÉ La présente étude visait à caractériser des isolats de P. aeruginosa dans deux unités de soins intensifs en Arabie saoudite et en Égypte. (who.int)
AadA21
- We first used a selective medium containing nalidixic acid 50 μg/mL plus 25 μg/mL of streptomycin, one of the two integrons carrying the aadA2 cassette. (cdc.gov)
Verona3
- Beginning in January 2016, Verona integron-mediated metallo-β-lactamase (VIM) producing carbapenem-resistant Enterobacteriaceae (CRE) were identified in Indiana. (cdc.gov)
- VIM, Verona integron‒encoded metallo-β-lactamase. (medscape.org)
- New Delhi Metallo-β-lactamase and Verona Integron-mediated Metallo-β-lactamase) in the United States are increasing," the advisory states. (medscape.com)
Class5
- Class 2 or 3 integrons were not detected. (cdc.gov)
- Cassette assortment in class 1 integrons was determined by using the primers 5′CS and 3′CS complementary to the 5′ and 3′ segments as described previously ( 3 ). (cdc.gov)
- With these primers, we obtained two amplification products of 1 kb and 1.7 kb for each strain, which suggested that all strains contained at least two class 1 integrons. (cdc.gov)
- The ereA2 cassette was first described in Providencia stuartii ( 8 ) in a class 1 integron. (cdc.gov)
- Our range of products include cd16 - multi-variable chemical indicator for eto (fda) type 4, cd20 - multi-variable chemical indicator for steam type 4, integron chemical indicator, it12 - chemical integrator for eto (fda) - class 5, it26-c - chemical integrator for steam (fda) type 5 and it27-7ys-emulator indicator for steam-type 6 chemical indicator. (starlinksresources.co.in)
Typhimurium1
- Integrons have been found in different nontyphoidal serovars of S. enterica and recently in serovar Typhimurium ( 3 ). (cdc.gov)
Cassettes1
- In bacterial genomes, they play a major role in the comings and goings of mobile genetic elements (MGEs), such as temperate phage genomes, integrated conjugative elements (ICEs) or integron cassettes. (biomedcentral.com)
Importance1
- More recently, our understanding of their importance in bacterial genome evolution has broadened with the discovery of larger integron structures, termed superintegrons. (nih.gov)
Metallo-beta-lactamase1
- In July 2010, CDC was notified of a patient with a carbapenem-resistant Klebsiella pneumoniae strain that produced a Verona integron-encoded metallo-beta-lactamase (VIM) carbapenemase [ 1 ] not reported previously among Enterobacteriaceae in the United States. (medscape.com)
Gram-negative2
- Integrons are efficient gene-capture systems by site-specific recombination and are involved in antimicrobial-drug resistance in gram-negative bacteria ( 2 ). (cdc.gov)
- From the Latin integrare (to make whole), integrons are systems for capturing and spreading antibiotic resistance genes among gram-negative bacteria. (cdc.gov)
Bacteria1
- Integrons are ancient structures that have been present in bacteria for millions of years, indicating that bacteria had the means of acquiring and disseminating antibiotic resistance long before humans developed antibiotics. (cdc.gov)
Resistance4
- Three classes of integrons are well characterized and are involved in antimicrobial resistance. (cdc.gov)
- All these integrons, except that of serovar Infantis, contained a streptomycin-spectinomycin resistance determinant, aadA2 or mostly aadA1 , alone or in combination with other gene cassettes. (cdc.gov)
- To determine whether the resistance determinants carried by the integrons were transferable, we performed a conjugation experiment from S. enterica serovar Keurmassar to an E. coli strain resistant to nalidixic acid. (cdc.gov)
- Integrons were first described by Stokes and Hall in 1989, although they clearly contributed to the first outbreaks of multidrug resistance in the 1950s. (cdc.gov)
Gene cassette2
- The gene cassette arrays of class 2 integrons were determined by PCR - RFLP and Sanger sequencing or next-generation sequencing when needed. (bvsalud.org)
- We showed a high frequency of class 2 integrons , as well as a diversity of gene cassette arrays, among Proteae. (bvsalud.org)
Evolution1
- Mazel D . Integrons: agents of bacterial evolution. (cdc.gov)
Mobile1
- A novel family of potentially mobile DNA elements encoding site-specific gene-integration functions: integrons. (cdc.gov)