A family of transmembrane glycoproteins (MEMBRANE GLYCOPROTEINS) consisting of noncovalent heterodimers. They interact with a wide variety of ligands including EXTRACELLULAR MATRIX PROTEINS; COMPLEMENT, and other cells, while their intracellular domains interact with the CYTOSKELETON. The integrins consist of at least three identified families: the cytoadhesin receptors(RECEPTORS, CYTOADHESIN), the leukocyte adhesion receptors (RECEPTORS, LEUKOCYTE ADHESION), and the VERY LATE ANTIGEN RECEPTORS. Each family contains a common beta-subunit (INTEGRIN BETA CHAINS) combined with one or more distinct alpha-subunits (INTEGRIN ALPHA CHAINS). These receptors participate in cell-matrix and cell-cell adhesion in many physiologically important processes, including embryological development; HEMOSTASIS; THROMBOSIS; WOUND HEALING; immune and nonimmune defense mechanisms; and oncogenic transformation.
Integrin beta-1 chains which are expressed as heterodimers that are noncovalently associated with specific alpha-chains of the CD49 family (CD49a-f). CD29 is expressed on resting and activated leukocytes and is a marker for all of the very late activation antigens on cells. (from: Barclay et al., The Leukocyte Antigen FactsBook, 1993, p164)
Adherence of cells to surfaces or to other cells.
An alpha integrin with a molecular weight of 160-kDa that is found in a variety of cell types. It undergoes posttranslational cleavage into a heavy and a light chain that are connected by disulfide bonds. Integrin alphaV can combine with several different beta subunits to form heterodimers that generally bind to RGD sequence-containing extracellular matrix proteins.
Cell-surface glycoprotein beta-chains that are non-covalently linked to specific alpha-chains of the CD11 family of leukocyte-adhesion molecules (RECEPTORS, LEUKOCYTE-ADHESION). A defect in the gene encoding CD18 causes LEUKOCYTE-ADHESION DEFICIENCY SYNDROME.
Integrin beta chains combine with integrin alpha chains to form heterodimeric cell surface receptors. Integrins have traditionally been classified into functional groups based on the identity of one of three beta chains present in the heterodimer. The beta chain is necessary and sufficient for integrin-dependent signaling. Its short cytoplasmic tail contains sequences critical for inside-out signaling.
Receptors such as INTEGRIN ALPHAVBETA3 that bind VITRONECTIN with high affinity and play a role in cell migration. They also bind FIBRINOGEN; VON WILLEBRAND FACTOR; osteopontin; and THROMBOSPONDINS.
An integrin beta subunit of approximately 85-kDa in size which has been found in INTEGRIN ALPHAIIB-containing and INTEGRIN ALPHAV-containing heterodimers. Integrin beta3 occurs as three alternatively spliced isoforms, designated beta3A-C.
An integrin alpha subunit that is unique in that it does not contain an I domain, and its proteolytic cleavage site is near the middle of the extracellular portion of the polypeptide rather than close to the membrane as in other integrin alpha subunits.
An integrin that binds to a variety of plasma and extracellular matrix proteins containing the conserved RGD amino acid sequence and modulates cell adhesion. Integrin alphavbeta3 is highly expressed in OSTEOCLASTS where it may play role in BONE RESORPTION. It is also abundant in vascular smooth muscle and endothelial cells, and in some tumor cells, where it is involved in angiogenesis and cell migration. Although often referred to as the vitronectin receptor there is more than one receptor for vitronectin (RECEPTORS, VITRONECTIN).
Glycoproteins found on the surfaces of cells, particularly in fibrillar structures. The proteins are lost or reduced when these cells undergo viral or chemical transformation. They are highly susceptible to proteolysis and are substrates for activated blood coagulation factor VIII. The forms present in plasma are called cold-insoluble globulins.
Cell surface receptor for LAMININ, epiligrin, FIBRONECTINS, entactin, and COLLAGEN. Integrin alpha3beta1 is the major integrin present in EPITHELIAL CELLS, where it plays a role in the assembly of BASEMENT MEMBRANE as well as in cell migration, and may regulate the functions of other integrins. Two alternatively spliced isoforms of the alpha subunit (INTEGRIN ALPHA3), are differentially expressed in different cell types.
A cell surface receptor mediating cell adhesion to the EXTRACELLULAR MATRIX and to other cells via binding to LAMININ. It is involved in cell migration, embryonic development, leukocyte activation and tumor cell invasiveness. Integrin alpha6beta1 is the major laminin receptor on PLATELETS; LEUKOCYTES; and many EPITHELIAL CELLS, and ligand binding may activate a number of signal transduction pathways. Alternative splicing of the cytoplasmic domain of the alpha6 subunit (INTEGRIN ALPHA6) results in the formation of A and B isoforms of the heterodimer, which are expressed in a tissue-specific manner.
Integrin alpha4beta1 is a FIBRONECTIN and VCAM-1 receptor present on LYMPHOCYTES; MONOCYTES; EOSINOPHILS; NK CELLS and thymocytes. It is involved in both cell-cell and cell- EXTRACELLULAR MATRIX adhesion and plays a role in INFLAMMATION, hematopoietic cell homing and immune function, and has been implicated in skeletal MYOGENESIS; NEURAL CREST migration and proliferation, lymphocyte maturation and morphogenesis of the PLACENTA and HEART.
Specific cell surface receptors which bind to FIBRONECTINS. Studies have shown that these receptors function in certain types of adhesive contact as well as playing a major role in matrix assembly. These receptors include the traditional fibronectin receptor, also called INTEGRIN ALPHA5BETA1 and several other integrins.
An integrin found in FIBROBLASTS; PLATELETS; MONOCYTES, and LYMPHOCYTES. Integrin alpha5beta1 is the classical receptor for FIBRONECTIN, but it also functions as a receptor for LAMININ and several other EXTRACELLULAR MATRIX PROTEINS.
The alpha subunits of integrin heterodimers (INTEGRINS), which mediate ligand specificity. There are approximately 18 different alpha chains, exhibiting great sequence diversity; several chains are also spliced into alternative isoforms. They possess a long extracellular portion (1200 amino acids) containing a MIDAS (metal ion-dependent adhesion site) motif, and seven 60-amino acid tandem repeats, the last 4 of which form EF HAND MOTIFS. The intracellular portion is short with the exception of INTEGRIN ALPHA4.
This integrin alpha subunit combines with INTEGRIN BETA1 to form a receptor (INTEGRIN ALPHA5BETA1) that binds FIBRONECTIN and LAMININ. It undergoes posttranslational cleavage into a heavy and a light chain that are connected by disulfide bonds.
A blood plasma glycoprotein that mediates cell adhesion and interacts with proteins of the complement, coagulation, and fibrinolytic cascade. (From Segen, Dictionary of Modern Medicine, 1992)
The movement of cells from one location to another. Distinguish from CYTOKINESIS which is the process of dividing the CYTOPLASM of a cell.
An integrin alpha subunit that occurs as alternatively spliced isoforms. The isoforms are differentially expressed in specific cell types and at specific developmental stages. Integrin alpha3 combines with INTEGRIN BETA1 to form INTEGRIN ALPHA3BETA1 which is a heterodimer found primarily in epithelial cells.
Large, noncollagenous glycoprotein with antigenic properties. It is localized in the basement membrane lamina lucida and functions to bind epithelial cells to the basement membrane. Evidence suggests that the protein plays a role in tumor invasion.
A meshwork-like substance found within the extracellular space and in association with the basement membrane of the cell surface. It promotes cellular proliferation and provides a supporting structure to which cells or cell lysates in culture dishes adhere.
Integrin alpha1beta1 functions as a receptor for LAMININ and COLLAGEN. It is widely expressed during development, but in the adult is the predominant laminin receptor (RECEPTORS, LAMININ) in mature SMOOTH MUSCLE CELLS, where it is important for maintenance of the differentiated phenotype of these cells. Integrin alpha1beta1 is also found in LYMPHOCYTES and microvascular endothelial cells, and may play a role in angiogenesis. In SCHWANN CELLS and neural crest cells, it is involved in cell migration. Integrin alpha1beta1 is also known as VLA-1 and CD49a-CD29.
An integrin alpha subunit that primarily associates with INTEGRIN BETA1 or INTEGRIN BETA4 to form laminin-binding heterodimers. Integrin alpha6 has two alternatively spliced isoforms: integrin alpha6A and integrin alpha6B, which differ in their cytoplasmic domains and are regulated in a tissue-specific and developmental stage-specific manner.
A 235-kDa cytoplasmic protein that is also found in platelets. It has been localized to regions of cell-substrate adhesion. It binds to INTEGRINS; VINCULIN; and ACTINS and appears to participate in generating a transmembrane connection between the extracellular matrix and the cytoskeleton.
Peptides composed of between two and twelve amino acids.
Surface ligands, usually glycoproteins, that mediate cell-to-cell adhesion. Their functions include the assembly and interconnection of various vertebrate systems, as well as maintenance of tissue integration, wound healing, morphogenic movements, cellular migrations, and metastasis.
Collagen receptors are cell surface receptors that modulate signal transduction between cells and the EXTRACELLULAR MATRIX. They are found in many cell types and are involved in the maintenance and regulation of cell shape and behavior, including PLATELET ACTIVATION and aggregation, through many different signaling pathways and differences in their affinities for collagen isoforms. Collagen receptors include discoidin domain receptors, INTEGRINS, and glycoprotein VI.
An integrin heterodimer widely expressed on cells of hematopoietic origin. CD11A ANTIGEN comprises the alpha chain and the CD18 antigen (ANTIGENS, CD18) the beta chain. Lymphocyte function-associated antigen-1 is a major receptor of T-CELLS; B-CELLS; and GRANULOCYTES. It mediates the leukocyte adhesion reactions underlying cytolytic conjugate formation, helper T-cell interactions, and antibody-dependent killing by NATURAL KILLER CELLS and granulocytes. Intracellular adhesion molecule-1 has been defined as a ligand for lymphocyte function-associated antigen-1.
An adhesion-promoting leukocyte surface membrane heterodimer. The alpha subunit consists of the CD11b ANTIGEN and the beta subunit the CD18 ANTIGEN. The antigen, which is an integrin, functions both as a receptor for complement 3 and in cell-cell and cell-substrate adhesive interactions.
An integrin found on fibroblasts, platelets, endothelial and epithelial cells, and lymphocytes where it functions as a receptor for COLLAGEN and LAMININ. Although originally referred to as the collagen receptor, it is one of several receptors for collagen. Ligand binding to integrin alpha2beta1 triggers a cascade of intracellular signaling, including activation of p38 MAP kinase.
Differentiation antigens residing on mammalian leukocytes. CD stands for cluster of differentiation, which refers to groups of monoclonal antibodies that show similar reactivity with certain subpopulations of antigens of a particular lineage or differentiation stage. The subpopulations of antigens are also known by the same CD designation.
The intracellular transfer of information (biological activation/inhibition) through a signal pathway. In each signal transduction system, an activation/inhibition signal from a biologically active molecule (hormone, neurotransmitter) is mediated via the coupling of a receptor/enzyme to a second messenger system or to an ion channel. Signal transduction plays an important role in activating cellular functions, cell differentiation, and cell proliferation. Examples of signal transduction systems are the GAMMA-AMINOBUTYRIC ACID-postsynaptic receptor-calcium ion channel system, the receptor-mediated T-cell activation pathway, and the receptor-mediated activation of phospholipases. Those coupled to membrane depolarization or intracellular release of calcium include the receptor-mediated activation of cytotoxic functions in granulocytes and the synaptic potentiation of protein kinase activation. Some signal transduction pathways may be part of larger signal transduction pathways; for example, protein kinase activation is part of the platelet activation signal pathway.
Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others.
An integrin alpha subunit that primarily combines with INTEGRIN BETA1 to form the INTEGRIN ALPHA2BETA1 heterodimer. It contains a domain which has homology to collagen-binding domains found in von Willebrand factor.
A group of three different alpha chains (CD11a, CD11b, CD11c) that are associated with an invariant CD18 beta chain (ANTIGENS, CD18). The three resulting leukocyte-adhesion molecules (RECEPTORS, LEUKOCYTE ADHESION) are LYMPHOCYTE FUNCTION-ASSOCIATED ANTIGEN-1; MACROPHAGE-1 ANTIGEN; and ANTIGEN, P150,95.
Cell surface glycoproteins on lymphocytes and other leukocytes that mediate adhesion to specialized blood vessels called high endothelial venules. Several different classes of lymphocyte homing receptors have been identified, and they appear to target different surface molecules (addressins) on high endothelial venules in different tissues. The adhesion plays a crucial role in the trafficking of lymphocytes.
Macromolecular organic compounds that contain carbon, hydrogen, oxygen, nitrogen, and usually, sulfur. These macromolecules (proteins) form an intricate meshwork in which cells are embedded to construct tissues. Variations in the relative types of macromolecules and their organization determine the type of extracellular matrix, each adapted to the functional requirements of the tissue. The two main classes of macromolecules that form the extracellular matrix are: glycosaminoglycans, usually linked to proteins (proteoglycans), and fibrous proteins (e.g., COLLAGEN; ELASTIN; FIBRONECTINS; and LAMININ).
A family of non-receptor, PROLINE-rich protein-tyrosine kinases.
Tetraspanin proteins found associated with LAMININ-binding INTEGRINS. The CD151 antigens may play a role in the regulation of CELL MOTILITY.
This intrgrin is a key component of HEMIDESMOSOMES and is required for their formation and maintenance in epithelial cells. Integrin alpha6beta4 is also found on thymocytes, fibroblasts, and Schwann cells, where it functions as a laminin receptor (RECEPTORS, LAMININ) and is involved in wound healing, cell migration, and tumor invasiveness.
The process in which substances, either endogenous or exogenous, bind to proteins, peptides, enzymes, protein precursors, or allied compounds. Specific protein-binding measures are often used as assays in diagnostic assessments.
Antibodies produced by a single clone of cells.
A group of INTEGRINS that includes the platelet outer membrane glycoprotein GPIIb-IIIa (PLATELET GLYCOPROTEIN GPIIB-IIIA COMPLEX) and the vitronectin receptor (RECEPTORS, VITRONECTIN). They play a major role in cell adhesion and serve as receptors for fibronectin, von Willebrand factor, and vitronectin.
A non-receptor protein tyrosine kinase that is localized to FOCAL ADHESIONS and is a central component of integrin-mediated SIGNAL TRANSDUCTION PATHWAYS. Focal adhesion kinase 1 interacts with PAXILLIN and undergoes PHOSPHORYLATION in response to adhesion of cell surface integrins to the EXTRACELLULAR MATRIX. Phosphorylated p125FAK protein binds to a variety of SH2 DOMAIN and SH3 DOMAIN containing proteins and helps regulate CELL ADHESION and CELL MIGRATION.
An anchoring junction of the cell to a non-cellular substrate. It is composed of a specialized area of the plasma membrane where bundles of the ACTIN CYTOSKELETON terminate and attach to the transmembrane linkers, INTEGRINS, which in turn attach through their extracellular domains to EXTRACELLULAR MATRIX PROTEINS.
A molecule that binds to another molecule, used especially to refer to a small molecule that binds specifically to a larger molecule, e.g., an antigen binding to an antibody, a hormone or neurotransmitter binding to a receptor, or a substrate or allosteric effector binding to an enzyme. Ligands are also molecules that donate or accept a pair of electrons to form a coordinate covalent bond with the central metal atom of a coordination complex. (From Dorland, 27th ed)
An alpha-integrin subunit found on lymphocytes, granulocytes, macrophages and monocytes. It combines with the integrin beta2 subunit (CD18 ANTIGEN) to form LYMPHOCYTE FUNCTION-ASSOCIATED ANTIGEN-1.
Also known as CD104 antigen, this protein is distinguished from other beta integrins by its relatively long cytoplasmic domain (approximately 1000 amino acids vs. approximately 50). Five alternatively spliced isoforms have been described.
Members of the integrin family appearing late after T-cell activation. They are a family of proteins initially identified at the surface of stimulated T-cells, but now identified on a variety of cell types. At least six VLA antigens have been identified as heterodimeric adhesion receptors consisting of a single common beta-subunit and different alpha-subunits.
The network of filaments, tubules, and interconnecting filamentous bridges which give shape, structure, and organization to the cytoplasm.
A polypeptide substance comprising about one third of the total protein in mammalian organisms. It is the main constituent of SKIN; CONNECTIVE TISSUE; and the organic substance of bones (BONE AND BONES) and teeth (TOOTH).
Glycoprotein molecules on the surface of cells that react with or bind to laminin whose function allows the binding of epithelial cells to the basement membrane. The molecular weight of this high-affinity receptor is 67 kD.
Established cell cultures that have the potential to propagate indefinitely.
Paxillin is a signal transducing adaptor protein that localizes to FOCAL ADHESIONS via its four LIM domains. It undergoes PHOSPHORYLATION in response to integrin-mediated CELL ADHESION, and interacts with a variety of proteins including VINCULIN; FOCAL ADHESION KINASE; PROTO-ONCOGENE PROTEIN PP60(C-SRC); and PROTO-ONCOGENE PROTEIN C-CRK.
The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.
A major adhesion-associated heterodimer molecule expressed by MONOCYTES; GRANULOCYTES; NK CELLS; and some LYMPHOCYTES. The alpha subunit is the CD11C ANTIGEN, a surface antigen expressed on some myeloid cells. The beta subunit is the CD18 ANTIGEN.
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
Family of proteins associated with the capacity of LEUKOCYTES to adhere to each other and to certain substrata, e.g., the C3bi component of complement. Members of this family are the LYMPHOCYTE FUNCTION-ASSOCIATED ANTIGEN-1; (LFA-1), the MACROPHAGE-1 ANTIGEN; (Mac-1), and the INTEGRIN ALPHAXBETA2 or p150,95 leukocyte adhesion protein. They all share a common beta-subunit which is the CD18 antigen. All three of the above antigens are absent in inherited LEUKOCYTE-ADHESION DEFICIENCY SYNDROME, which is characterized by recurrent bacterial infections, impaired pus formation, and wound healing as well as abnormalities in a wide spectrum of adherence-dependent functions of granulocytes, monocytes, and lymphoid cells.
CELL LINE derived from the ovary of the Chinese hamster, Cricetulus griseus (CRICETULUS). The species is a favorite for cytogenetic studies because of its small chromosome number. The cell line has provided model systems for the study of genetic alterations in cultured mammalian cells.
Platelet membrane glycoprotein complex important for platelet adhesion and aggregation. It is an integrin complex containing INTEGRIN ALPHAIIB and INTEGRIN BETA3 which recognizes the arginine-glycine-aspartic acid (RGD) sequence present on several adhesive proteins. As such, it is a receptor for FIBRINOGEN; VON WILLEBRAND FACTOR; FIBRONECTIN; VITRONECTIN; and THROMBOSPONDINS. A deficiency of GPIIb-IIIa results in GLANZMANN THROMBASTHENIA.
Surface glycoproteins on platelets which have a key role in hemostasis and thrombosis such as platelet adhesion and aggregation. Many of these are receptors.
Rare, autosomal recessive disorder caused by deficiency of the beta 2 integrin receptors (RECEPTORS, LEUKOCYTE-ADHESION) comprising the CD11/CD18 family of glycoproteins. The syndrome is characterized by abnormal adhesion-dependent functions, especially defective tissue emigration of neutrophils, leading to recurrent infection.
Granular leukocytes having a nucleus with three to five lobes connected by slender threads of chromatin, and cytoplasm containing fine inconspicuous granules and stainable by neutral dyes.
Cytokine-induced cell adhesion molecule present on activated endothelial cells, tissue macrophages, dendritic cells, bone marrow fibroblasts, myoblasts, and myotubes. It is important for the recruitment of leukocytes to sites of inflammation. (From Pigott & Power, The Adhesion Molecule FactsBook, 1993, p154)
A subtype of tetraspanin proteins that play a role in cell adhesion, cell motility, and tumor metastasis. CD9 antigens take part in the process of platelet activation and aggregation, the formation of paranodal junctions in neuronal tissue, and the fusion of sperm with egg.
A family of polypeptides purified from snake venoms, which contain the arginine-glycine-aspartic acid (RGD) sequence. The RGD tripeptide binds to integrin receptors and thus competitively inhibits normal integrin-ligand interactions. Disintegrins thus block adhesive functions and act as platelet aggregation inhibitors.
Single pavement layer of cells which line the luminal surface of the entire vascular system and regulate the transport of macromolecules and blood components.
Filamentous proteins that are the main constituent of the thin filaments of muscle fibers. The filaments (known also as filamentous or F-actin) can be dissociated into their globular subunits; each subunit is composed of a single polypeptide 375 amino acids long. This is known as globular or G-actin. In conjunction with MYOSINS, actin is responsible for the contraction and relaxation of muscle.
Technique using an instrument system for making, processing, and displaying one or more measurements on individual cells obtained from a cell suspension. Cells are usually stained with one or more fluorescent dyes specific to cell components of interest, e.g., DNA, and fluorescence of each cell is measured as it rapidly transverses the excitation beam (laser or mercury arc lamp). Fluorescence provides a quantitative measure of various biochemical and biophysical properties of the cell, as well as a basis for cell sorting. Other measurable optical parameters include light absorption and light scattering, the latter being applicable to the measurement of cell size, shape, density, granularity, and stain uptake.
A subfamily in the family MURIDAE, comprising the hamsters. Four of the more common genera are Cricetus, CRICETULUS; MESOCRICETUS; and PHODOPUS.
A cell-surface ligand involved in leukocyte adhesion and inflammation. Its production is induced by gamma-interferon and it is required for neutrophil migration into inflamed tissue.
The level of protein structure in which combinations of secondary protein structures (alpha helices, beta sheets, loop regions, and motifs) pack together to form folded shapes called domains. Disulfide bridges between cysteines in two different parts of the polypeptide chain along with other interactions between the chains play a role in the formation and stabilization of tertiary structure. Small proteins usually consist of only one domain but larger proteins may contain a number of domains connected by segments of polypeptide chain which lack regular secondary structure.
A cytoskeletal protein associated with cell-cell and cell-matrix interactions. The amino acid sequence of human vinculin has been determined. The protein consists of 1066 amino acid residues and its gene has been assigned to chromosome 10.
Cells grown in vitro from neoplastic tissue. If they can be established as a TUMOR CELL LINE, they can be propagated in cell culture indefinitely.
Protein kinases that catalyze the PHOSPHORYLATION of TYROSINE residues in proteins with ATP or other nucleotides as phosphate donors.
Specialized areas at the CELL MEMBRANE where a cell attaches to the EXTRACELLULAR MATRIX or other substratum.
Immunoglobulin molecules having a specific amino acid sequence by virtue of which they interact only with the ANTIGEN (or a very similar shape) that induced their synthesis in cells of the lymphoid series (especially PLASMA CELLS).
White blood cells. These include granular leukocytes (BASOPHILS; EOSINOPHILS; and NEUTROPHILS) as well as non-granular leukocytes (LYMPHOCYTES and MONOCYTES).
A darkly stained mat-like EXTRACELLULAR MATRIX (ECM) that separates cell layers, such as EPITHELIUM from ENDOTHELIUM or a layer of CONNECTIVE TISSUE. The ECM layer that supports an overlying EPITHELIUM or ENDOTHELIUM is called basal lamina. Basement membrane (BM) can be formed by the fusion of either two adjacent basal laminae or a basal lamina with an adjacent reticular lamina of connective tissue. BM, composed mainly of TYPE IV COLLAGEN; glycoprotein LAMININ; and PROTEOGLYCAN, provides barriers as well as channels between interacting cell layers.
Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
Connective tissue cells which secrete an extracellular matrix rich in collagen and other macromolecules.
Strains of mice in which certain GENES of their GENOMES have been disrupted, or "knocked-out". To produce knockouts, using RECOMBINANT DNA technology, the normal DNA sequence of the gene being studied is altered to prevent synthesis of a normal gene product. Cloned cells in which this DNA alteration is successful are then injected into mouse EMBRYOS to produce chimeric mice. The chimeric mice are then bred to yield a strain in which all the cells of the mouse contain the disrupted gene. Knockout mice are used as EXPERIMENTAL ANIMAL MODELS for diseases (DISEASE MODELS, ANIMAL) and to clarify the functions of the genes.
Plasma glycoprotein clotted by thrombin, composed of a dimer of three non-identical pairs of polypeptide chains (alpha, beta, gamma) held together by disulfide bonds. Fibrinogen clotting is a sol-gel change involving complex molecular arrangements: whereas fibrinogen is cleaved by thrombin to form polypeptides A and B, the proteolytic action of other enzymes yields different fibrinogen degradation products.
The uptake of naked or purified DNA by CELLS, usually meaning the process as it occurs in eukaryotic cells. It is analogous to bacterial transformation (TRANSFORMATION, BACTERIAL) and both are routinely employed in GENE TRANSFER TECHNIQUES.
Major constituent of the cytoskeleton found in the cytoplasm of eukaryotic cells. They form a flexible framework for the cell, provide attachment points for organelles and formed bodies, and make communication between parts of the cell possible.
A genetically related subfamily of RAP GTP-BINDING PROTEINS that share homology with RAS PROTEINS. They bind to Ras effectors but do not activate them, therefore they may antagonize the effects of RAS PROTEINS. This enzyme was formerly listed as EC 3.6.1.47.
The parts of a macromolecule that directly participate in its specific combination with another molecule.
The introduction of a phosphoryl group into a compound through the formation of an ester bond between the compound and a phosphorus moiety.
The movement of leukocytes in response to a chemical concentration gradient or to products formed in an immunologic reaction.
Transmembrane proteins consisting of a lectin-like domain, an epidermal growth factor-like domain, and a variable number of domains that are homologous to complement regulatory proteins. They are important cell adhesion molecules which help LEUKOCYTES attach to VASCULAR ENDOTHELIUM.
The process in which the neutrophil is stimulated by diverse substances, resulting in degranulation and/or generation of reactive oxygen products, and culminating in the destruction of invading pathogens. The stimulatory substances, including opsonized particles, immune complexes, and chemotactic factors, bind to specific cell-surface receptors on the neutrophil.
The lipid- and protein-containing, selectively permeable membrane that surrounds the cytoplasm in prokaryotic and eukaryotic cells.
Glycoproteins found on the membrane or surface of cells.
A purely physical condition which exists within any material because of strain or deformation by external forces or by non-uniform thermal expansion; expressed quantitatively in units of force per unit area.
Microscopy of specimens stained with fluorescent dye (usually fluorescein isothiocyanate) or of naturally fluorescent materials, which emit light when exposed to ultraviolet or blue light. Immunofluorescence microscopy utilizes antibodies that are labeled with fluorescent dye.
Highly specialized EPITHELIAL CELLS that line the HEART; BLOOD VESSELS; and lymph vessels, forming the ENDOTHELIUM. They are polygonal in shape and joined together by TIGHT JUNCTIONS. The tight junctions allow for variable permeability to specific macromolecules that are transported across the endothelial layer.
Serologic tests in which a positive reaction manifested by visible CHEMICAL PRECIPITATION occurs when a soluble ANTIGEN reacts with its precipitins, i.e., ANTIBODIES that can form a precipitate.
Proteins prepared by recombinant DNA technology.
Progressive restriction of the developmental potential and increasing specialization of function that leads to the formation of specialized cells, tissues, and organs.
Hexameric extracellular matrix glycoprotein transiently expressed in many developing organs and often re-expressed in tumors. It is present in the central and peripheral nervous systems as well as in smooth muscle and tendons. (From Kreis & Vale, Guidebook to the Extracellular Matrix and Adhesion Proteins, 1993, p93)
A cell line derived from cultured tumor cells.
A PROTEIN-TYROSINE KINASE family that was originally identified by homology to the Rous sarcoma virus ONCOGENE PROTEIN PP60(V-SRC). They interact with a variety of cell-surface receptors and participate in intracellular signal transduction pathways. Oncogenic forms of src-family kinases can occur through altered regulation or expression of the endogenous protein and by virally encoded src (v-src) genes.
An extracellular receptor specific for UROKINASE-TYPE PLASMINOGEN ACTIVATOR. It is attached to the cell membrane via a GLYCOSYLPHOSPHATIDYLINOSITOL LINKAGE and plays a role in the co-localization of urokinase-type plasminogen activator with PLASMINOGEN.
The process by which cells convert mechanical stimuli into a chemical response. It can occur in both cells specialized for sensing mechanical cues such as MECHANORECEPTORS, and in parenchymal cells whose primary function is not mechanosensory.
Theoretical representations that simulate the behavior or activity of biological processes or diseases. For disease models in living animals, DISEASE MODELS, ANIMAL is available. Biological models include the use of mathematical equations, computers, and other electronic equipment.
Epidermal cells which synthesize keratin and undergo characteristic changes as they move upward from the basal layers of the epidermis to the cornified (horny) layer of the skin. Successive stages of differentiation of the keratinocytes forming the epidermal layers are basal cell, spinous or prickle cell, and the granular cell.
Recombinant proteins produced by the GENETIC TRANSLATION of fused genes formed by the combination of NUCLEIC ACID REGULATORY SEQUENCES of one or more genes with the protein coding sequences of one or more genes.
Antibodies that inhibit the reaction between ANTIGEN and other antibodies or sensitized T-LYMPHOCYTES (e.g., antibodies of the IMMUNOGLOBULIN G class that compete with IGE antibodies for antigen, thereby blocking an allergic response). Blocking antibodies that bind tumors and prevent destruction of tumor cells by CYTOTOXIC T-LYMPHOCYTES have also been called enhancing antibodies. (Rosen et al., Dictionary of Immunology, 1989)
Any of several ways in which living cells of an organism communicate with one another, whether by direct contact between cells or by means of chemical signals carried by neurotransmitter substances, hormones, and cyclic AMP.
The phenomenon by which dissociated cells intermixed in vitro tend to group themselves with cells of their own type.
A non-fibrillar collagen found in the structure of BASEMENT MEMBRANE. Collagen type IV molecules assemble to form a sheet-like network which is involved in maintaining the structural integrity of basement membranes. The predominant form of the protein is comprised of two alpha1(IV) subunits and one alpha2(IV) subunit, however, at least six different alpha subunits can be incorporated into the heterotrimer.
Cells that line the inner and outer surfaces of the body by forming cellular layers (EPITHELIUM) or masses. Epithelial cells lining the SKIN; the MOUTH; the NOSE; and the ANAL CANAL derive from ectoderm; those lining the RESPIRATORY SYSTEM and the DIGESTIVE SYSTEM derive from endoderm; others (CARDIOVASCULAR SYSTEM and LYMPHATIC SYSTEM) derive from mesoderm. Epithelial cells can be classified mainly by cell shape and function into squamous, glandular and transitional epithelial cells.
The part of a cell that contains the CYTOSOL and small structures excluding the CELL NUCLEUS; MITOCHONDRIA; and large VACUOLES. (Glick, Glossary of Biochemistry and Molecular Biology, 1990)
A positive regulatory effect on physiological processes at the molecular, cellular, or systemic level. At the molecular level, the major regulatory sites include membrane receptors, genes (GENE EXPRESSION REGULATION), mRNAs (RNA, MESSENGER), and proteins.
An ERYTHROLEUKEMIA cell line derived from a CHRONIC MYELOID LEUKEMIA patient in BLAST CRISIS.
Chemically stimulated aggregation of cell surface receptors, which potentiates the action of the effector cell.
Members of the class of compounds composed of AMINO ACIDS joined together by peptide bonds between adjacent amino acids into linear, branched or cyclical structures. OLIGOPEPTIDES are composed of approximately 2-12 amino acids. Polypeptides are composed of approximately 13 or more amino acids. PROTEINS are linear polypeptides that are normally synthesized on RIBOSOMES.
Histochemical localization of immunoreactive substances using labeled antibodies as reagents.
Platelet membrane glycoprotein IIb is an integrin alpha subunit that heterodimerizes with INTEGRIN BETA3 to form PLATELET GLYCOPROTEIN GPIIB-IIIA COMPLEX. It is synthesized as a single polypeptide chain which is then postranslationally cleaved and processed into two disulfide-linked subunits of approximately 18 and 110 kDa in size.
A negatively-charged extracellular matrix protein that plays a role in the regulation of BONE metabolism and a variety of other biological functions. Cell signaling by osteopontin may occur through a cell adhesion sequence that recognizes INTEGRIN ALPHA-V BETA-3.
A heterodimeric protein that is a cell surface antigen associated with lymphocyte activation. The initial characterization of this protein revealed one identifiable heavy chain (ANTIGENS, CD98 HEAVY CHAIN) and an indeterminate smaller light chain. It is now known that a variety of light chain subunits (ANTIGENS, CD98 LIGHT CHAINS) can dimerize with the heavy chain. Depending upon its light chain composition a diverse array of functions can be found for this protein. Functions include: type L amino acid transport, type y+L amino acid transport and regulation of cellular fusion.
Specific molecular components of the cell capable of recognizing and interacting with a virus, and which, after binding it, are capable of generating some signal that initiates the chain of events leading to the biological response.
Non-nucleated disk-shaped cells formed in the megakaryocyte and found in the blood of all mammals. They are mainly involved in blood coagulation.
A ubiquitously expressed membrane glycoprotein. It interacts with a variety of INTEGRINS and mediates responses to EXTRACELLULAR MATRIX PROTEINS.
Cell adhesion molecule and CD antigen that serves as a homing receptor for lymphocytes to lymph node high endothelial venules.
A fungal metabolite that blocks cytoplasmic cleavage by blocking formation of contractile microfilament structures resulting in multinucleated cell formation, reversible inhibition of cell movement, and the induction of cellular extrusion. Additional reported effects include the inhibition of actin polymerization, DNA synthesis, sperm motility, glucose transport, thyroid secretion, and growth hormone release.
Proteins which are found in membranes including cellular and intracellular membranes. They consist of two types, peripheral and integral proteins. They include most membrane-associated enzymes, antigenic proteins, transport proteins, and drug, hormone, and lectin receptors.
Restoration of integrity to traumatized tissue.
Large, phagocytic mononuclear leukocytes produced in the vertebrate BONE MARROW and released into the BLOOD; contain a large, oval or somewhat indented nucleus surrounded by voluminous cytoplasm and numerous organelles.
The outer covering of the body that protects it from the environment. It is composed of the DERMIS and the EPIDERMIS.
Venous vessels in the umbilical cord. They carry oxygenated, nutrient-rich blood from the mother to the FETUS via the PLACENTA. In humans, there is normally one umbilical vein.
Cell surface proteins that bind signalling molecules external to the cell with high affinity and convert this extracellular event into one or more intracellular signals that alter the behavior of the target cell (From Alberts, Molecular Biology of the Cell, 2nd ed, pp693-5). Cell surface receptors, unlike enzymes, do not chemically alter their ligands.
Partial proteins formed by partial hydrolysis of complete proteins or generated through PROTEIN ENGINEERING techniques.
The fission of a CELL. It includes CYTOKINESIS, when the CYTOPLASM of a cell is divided, and CELL NUCLEUS DIVISION.
Conversion of an inactive form of an enzyme to one possessing metabolic activity. It includes 1, activation by ions (activators); 2, activation by cofactors (coenzymes); and 3, conversion of an enzyme precursor (proenzyme or zymogen) to an active enzyme.
A formylated tripeptide originally isolated from bacterial filtrates that is positively chemotactic to polymorphonuclear leucocytes, and causes them to release lysosomal enzymes and become metabolically activated.
The most common form of fibrillar collagen. It is a major constituent of bone (BONE AND BONES) and SKIN and consists of a heterotrimer of two alpha1(I) and one alpha2(I) chains.
A non-essential amino acid. In animals it is synthesized from PHENYLALANINE. It is also the precursor of EPINEPHRINE; THYROID HORMONES; and melanin.
A RHO GTP-BINDING PROTEIN involved in regulating signal transduction pathways that control assembly of focal adhesions and actin stress fibers. This enzyme was formerly listed as EC 3.6.1.47.
Calcium-dependent cell adhesion proteins. They are important in the formation of ADHERENS JUNCTIONS between cells. Cadherins are classified by their distinct immunological and tissue specificities, either by letters (E- for epithelial, N- for neural, and P- for placental cadherins) or by numbers (cadherin-12 or N-cadherin 2 for brain-cadherin). Cadherins promote cell adhesion via a homophilic mechanism as in the construction of tissues and of the whole animal body.
A ubiquitously expressed syndecan that is found in all stages of embryonic development and in most adult tissues. Syndecan-4 is found localized to focal adhesion sites in fibronectin-adherent cells and may play a role the process of CELL MIGRATION and CELL PROLIFERATION.
A genus of the family Muridae consisting of eleven species. C. migratorius, the grey or Armenian hamster, and C. griseus, the Chinese hamster, are the two species used in biomedical research.
The development of new BLOOD VESSELS during the restoration of BLOOD CIRCULATION during the healing process.
A light microscopic technique in which only a small spot is illuminated and observed at a time. An image is constructed through point-by-point scanning of the field in this manner. Light sources may be conventional or laser, and fluorescence or transmitted observations are possible.
A CD antigen that contains a conserved I domain which is involved in ligand binding. When combined with CD18 the two subunits form MACROPHAGE-1 ANTIGEN.
Any of the processes by which nuclear, cytoplasmic, or intercellular factors influence the differential control (induction or repression) of gene action at the level of transcription or translation.
A CELL LINE derived from human T-CELL LEUKEMIA and used to determine the mechanism of differential susceptibility to anti-cancer drugs and radiation.
A protein factor that regulates the length of R-actin. It is chemically similar, but immunochemically distinguishable from actin.
A family of crosslinking filament proteins encoded by distinct FLN genes. Filamins are involved in cell adhesion, spreading, and migration, acting as scaffolds for over 90 binding partners including channels, receptors, intracellular signaling molecules and transcription factors. Due to the range of molecular interactions, mutations in FLN genes result in anomalies with moderate to lethal consequences.
Any detectable and heritable change in the genetic material that causes a change in the GENOTYPE and which is transmitted to daughter cells and to succeeding generations.
Orientation of intracellular structures especially with respect to the apical and basolateral domains of the plasma membrane. Polarized cells must direct proteins from the Golgi apparatus to the appropriate domain since tight junctions prevent proteins from diffusing between the two domains.
A non-receptor protein-tyrosine kinase that is expressed primarily in the BRAIN; OSTEOBLASTS; and LYMPHOID CELLS. In the CENTRAL NERVOUS SYSTEM focal adhesion kinase 2 modulates ION CHANNEL function and MITOGEN-ACTIVATED PROTEIN KINASES activity.
An Ig superfamily transmembrane protein that localizes to junctional complexes that occur between ENDOTHELIAL CELLS and EPTHELIAL CELLS. The protein may play a role in cell-cell adhesion and is the primary site for the attachment of ADENOVIRUSES during infection.
Commonly observed structural components of proteins formed by simple combinations of adjacent secondary structures. A commonly observed structure may be composed of a CONSERVED SEQUENCE which can be represented by a CONSENSUS SEQUENCE.
Lymphocytes responsible for cell-mediated immunity. Two types have been identified - cytotoxic (T-LYMPHOCYTES, CYTOTOXIC) and helper T-lymphocytes (T-LYMPHOCYTES, HELPER-INDUCER). They are formed when lymphocytes circulate through the THYMUS GLAND and differentiate to thymocytes. When exposed to an antigen, they divide rapidly and produce large numbers of new T cells sensitized to that antigen.
Microscopy in which television cameras are used to brighten magnified images that are otherwise too dark to be seen with the naked eye. It is used frequently in TELEPATHOLOGY.
Methods for maintaining or growing CELLS in vitro.
Movement of tethered, spherical LEUKOCYTES along the endothelial surface of the microvasculature. The tethering and rolling involves interaction with SELECTINS and other adhesion molecules in both the ENDOTHELIUM and leukocyte. The rolling leukocyte then becomes activated by CHEMOKINES, flattens out, and firmly adheres to the endothelial surface in preparation for transmigration through the interendothelial cell junction. (From Abbas, Cellular and Molecular Immunology, 3rd ed)
The quantity of volume or surface area of CELLS.
RNA sequences that serve as templates for protein synthesis. Bacterial mRNAs are generally primary transcripts in that they do not require post-transcriptional processing. Eukaryotic mRNA is synthesized in the nucleus and must be exported to the cytoplasm for translation. Most eukaryotic mRNAs have a sequence of polyadenylic acid at the 3' end, referred to as the poly(A) tail. The function of this tail is not known for certain, but it may play a role in the export of mature mRNA from the nucleus as well as in helping stabilize some mRNA molecules by retarding their degradation in the cytoplasm.
The phenotypic manifestation of a gene or genes by the processes of GENETIC TRANSCRIPTION and GENETIC TRANSLATION.
Fibers composed of MICROFILAMENT PROTEINS, which are predominately ACTIN. They are the smallest of the cytoskeletal filaments.
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
Solutions or mixtures of toxic and nontoxic substances elaborated by snake (Ophidia) salivary glands for the purpose of killing prey or disabling predators and delivered by grooved or hollow fangs. They usually contain enzymes, toxins, and other factors.
A large family of MONOMERIC GTP-BINDING PROTEINS that are involved in regulation of actin organization, gene expression and cell cycle progression. This enzyme was formerly listed as EC 3.6.1.47.
The development of anatomical structures to create the form of a single- or multi-cell organism. Morphogenesis provides form changes of a part, parts, or the whole organism.
A transmembrane glycoprotein subunit that can dimerize with a variety of light chain subunits (ANTIGENS, CD98 LIGHT CHAINS). This protein subunit serves a diverse array of functions including amino acid transport and cell fusion. Its function is altered depending which of the light chain subunits it interacts with.
Conjugated proteins in which mucopolysaccharides are combined with proteins. The mucopolysaccharide moiety is the predominant group with the protein making up only a small percentage of the total weight.
Venoms from SNAKES of the viperid family. They tend to be less toxic than elapid or hydrophid venoms and act mainly on the vascular system, interfering with coagulation and capillary membrane integrity and are highly cytotoxic. They contain large amounts of several enzymes, other factors, and some toxins.

Primary haemostasis: sticky fingers cement the relationship. (1/5403)

Platelet aggregation to form a haemostatic plug, or thrombus, plays a key role in preventing bleeding from a wound. Recent studies have provided new insights into how platelet receptors are deployed during the interactions with the vascular subendothelial matrix that lead to haemostatic plug formation.  (+info)

The LIM-only protein PINCH directly interacts with integrin-linked kinase and is recruited to integrin-rich sites in spreading cells. (2/5403)

PINCH is a widely expressed and evolutionarily conserved protein comprising primarily five LIM domains, which are cysteine-rich consensus sequences implicated in mediating protein-protein interactions. We report here that PINCH is a binding protein for integrin-linked kinase (ILK), an intracellular serine/threonine protein kinase that plays important roles in the cell adhesion, growth factor, and Wnt signaling pathways. The interaction between ILK and PINCH has been consistently observed under a variety of experimental conditions. They have interacted in yeast two-hybrid assays, in solution, and in solid-phase-based binding assays. Furthermore, ILK, but not vinculin or focal adhesion kinase, has been coisolated with PINCH from mammalian cells by immunoaffinity chromatography, indicating that PINCH and ILK associate with each other in vivo. The PINCH-ILK interaction is mediated by the N-terminal-most LIM domain (LIM1, residues 1 to 70) of PINCH and multiple ankyrin (ANK) repeats located within the N-terminal domain (residues 1 to 163) of ILK. Additionally, biochemical studies indicate that ILK, through the interaction with PINCH, is capable of forming a ternary complex with Nck-2, an SH2/SH3-containing adapter protein implicated in growth factor receptor kinase and small GTPase signaling pathways. Finally, we have found that PINCH is concentrated in peripheral ruffles of cells spreading on fibronectin and have detected clusters of PINCH that are colocalized with the alpha5beta1 integrins. These results demonstrate a specific protein recognition mechanism utilizing a specific LIM domain and multiple ANK repeats and suggest that PINCH functions as an adapter protein connecting ILK and the integrins with components of growth factor receptor kinase and small GTPase signaling pathways.  (+info)

Blocking very late antigen-4 integrin decreases leukocyte entry and fatty streak formation in mice fed an atherogenic diet. (3/5403)

Atherosclerotic lesion development is characterized by the recruitment of leukocytes, principally monocytes, to the vessel wall. Considerable interest has been focused on the adhesion molecule(s) involved in leukocyte/endothelial interactions. The goal of the present study was to determine the role of the very late antigen-4 (VLA-4) integrin/ligand interaction in fatty streak development using murine models. Because alpha4 null mice are not viable, a peptidomimetic was used to block VLA-4-mediated leukocyte binding. The ability of a synthetic peptidomimetic of connecting segment-1 (CS-1 peptide) to block the recruitment of leukocytes and the accumulation of lipid in the aortic sinus of either wild-type mice (strain C57BL/6J) or mice with a low-density lipoprotein null mutation (LDLR-/-) maintained on an atherogenic diet was assessed. The active (Ac) CS-1 peptide or scrambled (Sc) CS-1 peptide was delivered subcutaneously into mice using a mini osmotic pump. Mice were exposed to the peptide for 24 to 36 hours before the onset of the atherogenic diet. In C57BL/6J mice, leukocyte entry into the aortic sinus, as assessed by en face preparations, was inhibited by the active peptide (Ac=28+/-4, Sc=54+/-6 monocytes/valve; P=0.004). Additionally, frozen sections stained with Oil Red O were analyzed to assess lipid accumulation in the aortic sinus. C57BL/6J mice that received the (Ac) compound demonstrated significantly reduced lesion areas as compared with mice that received the (Sc) peptide (Ac=4887+/-4438 microm2, Sc=15 009 +/-5619 microm2; P<0.0001). In a separate study, LDLR-/- mice were implanted with pumps containing either the (Ac) or (Sc) peptide before initiation of the atherogenic diet. Because LDLR-/- mice fed a chow diet displayed small lesions at 14 weeks, the effects of the peptide seen in these animals represented a change in early lipid accumulation rather than initiation. By using whole-mount preparations, the (Ac) but not the (Sc) peptide significantly reduced the area of lipid accumulation in the aortic sinus, resulting in an approximate 66% decrease. Plasma analysis from all studies revealed concentrations of peptide to be present at levels previously determined by in vitro analysis to block adhesion. (Ac) CS-1 peptide, which blocks VLA-4 on the leukocyte surface, is effective in reducing leukocyte recruitment and lipid accumulation in the aortic sinus. The present study provides in vivo evidence that the VLA-4 integrin plays an important role in the initiation of the atherosclerotic lesion and lipid accumulation, and it suggests a potential therapeutic strategy for this disease.  (+info)

Integrin subunit gene expression is regionally differentiated in adult brain. (4/5403)

Integrins are a diverse family of heterodimeric (alphabeta) adhesion receptors recently shown to be concentrated within synapses and involved in the consolidation of long-term potentiation. Whether neuronal types or anatomical systems in the adult rat brain are coded by integrin type was studied in the present experiments by mapping the relative densities of mRNAs for nine alpha and four beta subunits. Expression patterns were markedly different and in some regions complementary. General results and areas of notable labeling were as follows: alpha1-limited neuronal expression, neocortical layer V, hippocampal CA3; alpha3 and alpha5-diffuse neuronal and glial labeling, Purkinje cells, hippocampal stratum pyramidale, locus coeruleus (alpha3); alpha4- discrete limbic regions, olfactory cortical layer II, hippocampal CA2; alpha6-most prominently neuronal, neocortical subplate, endopiriform, subiculum; alpha7-discrete, all neocortical layers, hippocampal granule cells and CA3, cerebellar granule and Purkinje cells, all efferent cranial nerve nuclei; alpha8-discrete neuronal, deep cortex, hippocampal CA1, basolateral amygdala, striatum; alphaV-all cortical layers, striatum, Purkinje cells; beta4-dentate gyrus granule cells; beta5-broadly distributed, neocortex, medial amygdala, cerebellar granule and Purkinje cells, efferent cranial nerve nuclei; alpha2, beta2, and beta3-mRNAs not detected. These results establish that brain subfields express different balances of integrin subunits and thus different integrin receptors. Such variations will determine which matrix proteins are recognized by neurons and the types of intraneuronal signaling generated by matrix binding. They also could generate important differences in synaptic plasticity across brain systems.  (+info)

The integrin alpha v beta 6 binds and activates latent TGF beta 1: a mechanism for regulating pulmonary inflammation and fibrosis. (5/5403)

Transforming growth factor beta (TGF beta) family members are secreted in inactive complexes with a latency-associated peptide (LAP), a protein derived from the N-terminal region of the TGF beta gene product. Extracellular activation of these complexes is a critical but incompletely understood step in regulation of TGF beta function in vivo. We show that TGF beta 1 LAP is a ligand for the integrin alpha v beta 6 and that alpha v beta 6-expressing cells induce spatially restricted activation of TGF beta 1. This finding explains why mice lacking this integrin develop exaggerated inflammation and, as we show, are protected from pulmonary fibrosis. These data identify a novel mechanism for locally regulating TGF beta 1 function in vivo by regulating expression of the alpha v beta 6 integrin.  (+info)

Cell adhesion regulates the interaction between the docking protein p130(Cas) and the 14-3-3 proteins. (6/5403)

Integrin ligand binding induces a signaling complex formation via the direct association of the docking protein p130(Cas) (Cas) with diverse molecules. We report here that the 14-3-3zeta protein interacts with Cas in the yeast two-hybrid assay. We also found that the two proteins associate in mammalian cells and that this interaction takes place in a phosphoserine-dependent manner, because treatment of Cas with a serine phosphatase greatly reduced its ability to bind 14-3-3zeta. Furthermore, the Cas-14-3-3zeta interaction was found to be regulated by integrin-mediated cell adhesion. Thus, when cells are detached from the extracellular matrix, the binding of Cas to 14-3-3zeta is greatly diminished, whereas replating the cells onto fibronectin rapidly induces the association. Consistent with these results, we found that the subcellular localization of Cas and 14-3-3 is also regulated by integrin ligand binding and that the two proteins display a significant co-localization during cell attachment to the extracellular matrix. In conclusion, our results demonstrate that 14-3-3 proteins participate in integrin-activated signaling pathways through their interaction with Cas, which, in turn, may contribute to important biological responses regulated by cell adhesion to the extracellular matrix.  (+info)

Tyrosine phosphorylation of SLP-76 is downstream of Syk following stimulation of the collagen receptor in platelets. (7/5403)

Collagen-related peptide (CRP), a collagen homologue, induces platelet activation through a tyrosine kinase-dependent pathway, leading to sequential tyrosine phosphorylation of Fc receptor (FcR) gamma-chain, Syk, and phospholipase C-gamma2. Here we report that CRP and the platelet low affinity immune receptor FcgammaRIIA stimulate tyrosine phosphorylation of the T cell adapter SLP-76, whereas the G protein-coupled receptor agonist thrombin induces only minor tyrosine phosphorylation. This suggests that SLP-76 has a specific role downstream of receptors that signal via an immunoreceptor tyrosine-based activation motif. Immunoprecipitation studies demonstrate association of SLP-76 with SLAP-130, Vav, Fyn, Lyn, and the FcR gamma-chain in CRP-stimulated platelets. Several of these proteins, including SLP-76, undergo tyrosine phosphorylation in in vitro kinase assays performed on SLP-76 immunoprecipitates. Tyrosine phosphorylation of all of these proteins in the in vitro kinase assay was abrogated by the Src family kinase inhibitor PP1, suggesting that it is mediated by either Fyn or Lyn. The physiological significance of this is uncertain, however, since tyrosine phosphorylation of SLP-76 in vivo is not altered in either Fyn- or Lyn-deficient platelets. CRP stimulation of Syk-deficient platelets demonstrated that in vivo tyrosine phosphorylation of SLP-76 is downstream of Syk. The absence of Syk in the SLP-76 immunoprecipitates raises the possibility that another protein is responsible for bringing SLP-76 to Syk. Candidates for this include those proteins that co-immunoprecipitate with SLP-76, including the FcR gamma-chain. Tyrosine phosphorylation of PLC-gamma2 and Ca2+ mobilization is markedly attenuated in SLP-76-deficient platelets following CRP stimulation, suggesting that the adapter plays a critical role in the regulation of the phospholipase. The increase in tyrosine phosphorylation of SLAP-130 in response to CRP is also inhibited in SLP-76-deficient platelets, placing it downstream of SLP-76. This work identifies SLP-76 as an important adapter molecule that is regulated by Syk and lies upstream of SLAP-130 and PLC-gamma2 in CRP-stimulated platelets.  (+info)

Expression pattern of integrin adhesion molecules in endometriosis and human endometrium. (8/5403)

Integrins are cell adhesion molecules that undergo cell-specific dynamic changes during the normal menstrual cycle in the human endometrium. Here, using immunohistochemistry, we have investigated the expression pattern of the integrins alphav, alpha2beta1, alpha3beta1, alpha3, alpha6, beta1, beta2 and beta3 in the human ectopic endometrium of 30 patients and in nine cases in the corresponding eutopic endometrium. The biopsies were obtained during the early or late follicular phase (25 cases), during the corpus luteum phase (four cases) and in one case after 6 months' treatment with a gonadotrophin releasing hormone (GnRH) agonist. The integrin expression was independent of the ovarian steroid situation at the time of biopsy. The integrin alpha6 was expressed in all endometriotic and endometrium samples. The integrin alpha3 was absent in all endometrium tissues of patients with endometriosis. However, the corresponding endometriotic lesions re-expressed this adhesion molecule in 15 cases. No change in integrin beta3 expression pattern could be demonstrated in either endometriotic lesions or endometrium samples, regardless of the menstrual cycle phase. A correlation between serum oestradiol and progesterone concentrations and the expression of the investigated integrins was not observed, thus indicating that these two hormones play a minor role in the regulation of the cell adhesion molecules examined. Our investigation suggests that endometriosis is a dedifferentiated disease as it expressed different integrins in comparison with the eutopic endometrium, and independently of the hormonal situation. The ability of endometriotic tissues to express integrins may explain the high recurrence rates in patients with endometriosis, as these samples retain their adhesion potency after retrograde menstruation and are thus able to establish cell-cell and cell-matrix interactions with the surrounding peritoneum.  (+info)

ITAGV encodes integrin alpha chain V. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. The I-domain containing integrin alpha V undergoes post-translational cleavage to yield disulfide-linked heavy and light chains, that combine with multiple integrin beta chains to form different integrins. Among the known associating beta chains (beta chains 1,3,5,6, and 8; ITGB1, ITGB3, ITGB5, ITGB6, and ITGB8), each can interact with extracellular matrix ligands; the alpha V beta 3 integrin, perhaps the most studied of these, is referred to as the Vitronectin receptor (VNR). In addition to adhesion, many integrins are known to facilitate signal transduction. The ITGB3 protein product is the integrin beta chain beta 3. Integrins are integral cell-surface proteins composed of an alpha chain and a beta chain. A given chain may combine with multiple partners resulting in different integrins. Integrin beta 3 is found along with the alpha IIb chain in ...
Leukocyte adhesion during hypoxia is mediated by HIF-1-dependent induction of beta2 integrin gene expression.s profile, publications, research topics, and co-authors
Integrin receptors regulate cell fate by coupling the binding of extracellular adhesion proteins to the assembly of intracellular cytoskeletal and signaling complexes. A detailed, integrative view of adhesion complexes will provide insight into the molecular mechanisms that control cell morphology, survival, movement, and differentiation. To date, membrane receptor-associated signaling complexes have been refractory to proteomic analysis because of their inherent lability and inaccessibility. We developed a methodology to isolate ligand-induced integrin adhesion complexes, and we used this technique to analyze the composition of complexes associated with multiple receptor-ligand pairs and define core and receptor-specific subnetworks. In particular, we identified regulator of chromosome condensation-2 (RCC2) as a component of fibronectin-activated signaling pathways that regulate directional cell movement. The development of this proteomics pipeline provides the means to investigate the ...
TY - JOUR. T1 - Subclassification, molecular structure, function and ligand in integrin superfamily. AU - Matsuura, N.. AU - Takada, Y.. PY - 1995/7. Y1 - 1995/7. N2 - Integrins are the major family of cell surface receptors that mediate adhesion to the extracellular matrix and sometimes cell-cell adhesive interactions. These integrin-mediated adhesive interactions are involved in the regulation of many cellular functions, including embryonic development, tumor cell growth and metastasis, programmed cell death, hemostasis, inflammation, immune reaction, bone reabsorption, etc. Integrins are composed of alpha and beta transmembrane subunits selected from among 16 alpha and 8 beta subunits that heterodimerize to produce more than 20 different receptors which bind specific ligands. Ligand binding sites have been clarified by chimera integrin protein in some integrins. Integrins link to intracellular cytoskeletal complexes and bundles of actin filaments. There have been many reports about ...
Adhesion of cells to extracellular matrix proteins is mediated, in large part, by transmembrane receptors of the integrin family. The identification of specific integrins expressed in early embryos is an important first step to understanding the roles of these receptors in developmental processes. We have used polymerase chain reaction methods and degenerate oligodeoxynucleotide primers to identify and clone Xenopus integrin alpha subunits from neurula-stage (stage 17) cDNA. Partial cDNAs encoding integrin subunits alpha 2, alpha 3, alpha 4, alpha 5, alpha 6 and an alpha IIb-related subunit were cloned and used to investigate integrin mRNA expression in early embryos by RNase protection assay and whole-mount in situ hybridization methods. Considerable integrin diversity is apparent early in development with integrins alpha 2, alpha 3, alpha 4, alpha 5 and alpha 6 each expressed by the end of gastrulation. Both alpha 3 and alpha 5 are expressed as maternal mRNAs. Zygotic expression of alpha 2, ...
alpha 4 integrins are cell surface receptors that mediate cell-extracellular matrix (ECM) and cell-cell adhesions by interacting with fibronectin (FN) and vascular cell adhesion molecule 1 (VCAM-1), respectively. We have generated a null mutation in the gene for the alpha 4 integrin subunit. Homozygous null embryos express no alpha 4 integrins and show two unexpected defects, both of which lead to embryonic lethality. The first defect is failure of fusion of the allantois with the chorion during placentation. The second is in the development of the epicardium and coronary vessels leading to cardiac hemorrhage. Both processes clearly involve alpha 4 integrin interactions that were previously unsuspected. alpha 4 integrin and VCAM-1 are expressed at the sites of these interactions. These results raise the possibility of abortifacients targeting alpha 4 integrins, and raise serious questions about potential side effects of drugs currently being designed to block alpha 4 integrin functions in ...
The product of this gene belongs to the integrin alpha chain family. Integrins are heterodimeric integral membrane proteins composed of an alpha subunit and a beta subunit that function in cell surface adhesion and signaling. The encoded preproprotein is proteolytically processed to generate light and heavy chains that comprise the alpha 5 subunit. This subunit associates with the beta 1 subunit to form a fibronectin receptor. This integrin may promote tumor invasion, and higher expression of this gene may be correlated with shorter survival time in lung cancer patients. Note that the integrin alpha 5 and integrin alpha V subunits are encoded by distinct genes. [provided by RefSeq, Oct 2015 ...
|strong|Monoclonal antibody which detects the ectodomain of integrin aV.|/strong| |strong|Background and Research Application|/strong| Integrins are heterodimeric cell surface receptors composed of alpha
Integrin alpha-IIb is a protein that in humans is encoded by the ITGA2B gene. ITGA2B, also known as CD41, encodes integrin alpha chain 2b. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. Alpha chain 2b undergoes post-translational cleavage to yield disulfide-linked light and heavy chains that join with beta 3 to form a fibrinogen receptor expressed in platelets that plays a crucial role in coagulation. Mutations that interfere with this role result in thrombasthenia. In addition to adhesion, integrins are known to participate in cell-surface mediated signalling.[5] ...
Integrins are cell adhesion receptors which mediate interactions between the extracellular matrix and the actin cytoskeleton. They are heterodimers composed of α and β subunits. As adhesion receptors, integrins are important for cell-cell and cell-matrix interactions and therefore are essential for the structural integrity of an organ. Moreover, integrin-extracellular matrix interactions play important roles in the coordinated integration of external and internal cues that are essential for proper development. β1 integrin is the most widely expressed integrin and controls various developmental processes, including neurogenesis, chondrogenesis, skin and hair follicle morphogenesis, and myoblast fusion. To determine the role of β1 integrin in normal development of the mouse mammary gland, with a particular emphasis on how β1 integrins influcence proliferation, differentiation and apoptosis; we examined the consequence of conditional deletion of β1 integrin in mammary epithelia. ...
Luo, B.-H., Carman, C.V. & Springer, T.A. Structural basis of integrin regulation and signaling. Annu Rev Immunol. 25, 619-47 (2007).
Integrin alpha 6 antibody [MP 4F10] (integrin, alpha 6) for FACS, IHC-Fr, IP. Anti-Integrin alpha 6 mAb (GTX40142) is tested in Human samples. 100% Ab-Assurance.
The mouse monoclonal antibody recognizes human Integrin alpha L/CD11a. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain.
Integrins are cell‐surface adhesion molecules formed from eight different β chains and 18 different α chains that assemble as heterodimeric transmembrane receptors to mediate cell-cell and cell-matrix interactions
Abcam provides specific protocols for Anti-Integrin alpha 9+beta 1 antibody [Y9A2] (ab27947) : Flow cytometry protocols, Immunoprecipitation protocols…
Anti-Integrin alpha 2 antibody conjugated to FITC [AK7] validated for Flow Cyt and tested in Human. Referenced in 2 publications. Immunogen corresponding to…
Gene Information This gene product belongs to the integrin alpha chain family. Integrins are heterodimeric integral membrane glycoproteins composed of a distinct alpha chain and a common beta chain. They are found on a wide variety of cell types including T cells fibroblasts and platelets. Integrins are involved in cell adhesion and also participate in cell-surface mediated signalling. [provided by RefSeq Jul 2008]. ...
Integrins are heterodimeric transmembrane receptor proteins that mediate numerous cellular processes including cell adhesion, cytoskeletal rearrangement, and activation of cell signaling pathways. Integrins are composed of alpha and beta subunits. This gene encodes the alpha 8 subunit of the heterodimeric integrin alpha8beta1 protein. The encoded protein is a single-pass type 1 membrane protein that contains multiple FG-GAP repeats. This repeat is predicted to fold into a beta propeller structure. This gene regulates the recruitment of mesenchymal cells into epithelial structures, mediates cell-cell interactions, and regulates neurite outgrowth of sensory and motor neurons. The integrin alpha8beta1 protein thus plays an important role in wound-healing and organogenesis. Mutations in this gene have been associated with renal hypodysplasia/aplasia-1 (RHDA1) and with several animal models of chronic kidney disease. Alternate splicing results in multiple transcript variants encoding distinct ...
Clone REA718 recognizes the human integrin β5 antigen, a 95 kDa glycoprotein single-pass type I membrane protein. Integrins are a family of transmembrane receptors that mediate adhesion of cells to extracellular matrices, as well as intercellular interactions. These interactions transduce signals that control complex cell functions such as proliferation, differentiation, and survival, and require the regulation of gene expression. Integrins are heterodimeric glycoprotein receptors and exist as non-covalently bound α and β subunits. The integrin αV/β5 heterodimer is found on many types of tissue cells, such as epithelial cells, endothelial cells, keratinocytes, and osteoblastic cells. It is a receptor for fibronectin and vitronectin and acts as a receptor for adenovirus type C. Additional information: Clone REA718 displays negligible binding to Fc receptors. - Lëtzebuerg
Clone REA718 recognizes the human integrin β5 antigen, a 95 kDa glycoprotein single-pass type I membrane protein. Integrins are a family of transmembrane receptors that mediate adhesion of cells to extracellular matrices, as well as intercellular interactions. These interactions transduce signals that control complex cell functions such as proliferation, differentiation, and survival, and require the regulation of gene expression. Integrins are heterodimeric glycoprotein receptors and exist as non-covalently bound α and β subunits. The integrin αV/β5 heterodimer is found on many types of tissue cells, such as epithelial cells, endothelial cells, keratinocytes, and osteoblastic cells. It is a receptor for fibronectin and vitronectin and acts as a receptor for adenovirus type C. Additional information: Clone REA718 displays negligible binding to Fc receptors. - Nederland
Integrin alpha V兔单克隆抗体[EPR5583](ab124968)可与小鼠, 大鼠, 人样本反应并经WB, Flow Cyt实验严格验证。所有产品均提供质保服务,中国75%以上现货。
The Integrin family proteins are heterodimeric transmembrane receptors composed of an alpha and a beta subunit. The Integrin alpha M subunit, also known as MAC-1 alpha subunit or CD11b, combines with the Integrin beta 2 subunit (CD18) to form the non-covalent heterodimer Integrin alpha M/ beta 2, also known as MAC-1 and Complement Receptor type 3 (CR3). Integrin alpha M/ beta 2 is expressed on granulocytes, macrophages, dendritic cells and natural killer cells. Upon activation, alpha M/ beta 2 can bind several ligands (including ICAM-1, Fibrinogen and the C3 Complement Fragment C3bi) to mediate phagocyte adhesion, migration and ingestion of complement-opsonized particles ...
Monoclonal antibody (mAb) AP7.4 is an anti-integrin antibody recombinantly expressed in Escherichia coli specific to alphavbeta3. It is known that in a variety of RGD-containing molecules, ligand specificity is regulated by structural determinants within the immediate vicinity of the RGD sequence. To better understand the role of the RGD sequence in integrin specificity, we report here the three-dimensional structure of Fab of mAb AP7.4 to a resolution of 2.25 A. The crystals belong to a triclinic space group P1 and the volume of the unit cell is consistent with the presence of two Fab molecules in it. The RGD sequence is located at the tip of a flexible loop in the complementary determining region (CDR-3) of the heavy chain. It has been shown that specific recognition of RGD ligands by their receptors is influenced mainly by the conformation of the tripeptide RGD and the amino acid residues flanking it on either side. Hence, the flexibility of the RGD-carrying loop observed in the crystal ...
Integrins are heterodimeric cell surface receptors composed of alpha and beta subunits, which mediate cell-cell and cell-extracellular matrix attachments. Aberrant
1. Lu X, Lu D, Scully M, Kakkar V. The role of integrins in cancer and the development of anti-integrin therapeutic agents for cancer therapy. Perspect Medicin Chem. 2008;2:57-73 2. Hynes RO. Integrins: a family of cell surface receptors. Cell. 1987;48:549-54 3. Hynes RO. Integrins: versatility, modulation, and signaling in cell adhesion. Cell. 1992;69:11-25 4. Serini G, Valdembri D, Bussolino F. Integrins and angiogenesis: a sticky business. Exp Cell Res. 2006;312:651-8 5. Brakebusch C, Bouvard D, Stanchi F, Sakai T, Fassler R. Integrins in invasive growth. J Clin Invest. 2002;109:999-1006 6. Hynes RO. Integrins: bidirectional, allosteric signaling machines. Cell. 2002;110:673-87 7. Vogel V, Sheetz M. Local force and geometry sensing regulate cell functions. Nat Rev Mol Cell Biol. 2006;7:265-75 8. Ginsberg MH, Partridge A, Shattil SJ. Integrin regulation. Curr Opin Cell Biol. 2005;17:509-16 9. Springer TA. Complement and the multifaceted functions of VWA and integrin I domains. Structure. ...
Rapid progress has been made in the understanding of the molecular interactions that result in cell adhesion. Many adhesive proteins present in extracellular matrices and in the blood contain the tripeptide arginine-glycine-aspartic acid (RGD) as their cell recognition site. These proteins include fibronectin, vitronectin, osteopontin, collagens, thrombospondin, fibrinogen, and von Willebrand factor. The RGD sequences of each of the adhesive proteins are recognized by at least one member of a family of structurally related receptors, integrins, which are heterodimeric proteins with two membrane-spanning subunits. Some of these receptors bind to the RGD sequence of a single adhesion protein only, whereas others recognize groups of them. The conformation of the RGD sequence in the individual proteins may be critical to this recognition specificity. On the cytoplasmic side of the plasma membrane, the receptors connect the extracellular matrix to the cytoskeleton. More than ten proved or suspected ...
Integrin alpha E beta 7 Peptides and Proteins available through Novus Biologicals. Browse our Integrin alpha E beta 7 Peptides and Proteins all backed by our Guarantee+.
Integrins are heterodimeric proteins made up of alpha and beta subunits. At least 18 alpha and 8 beta subunits have been described in mammals. Integrin family members are membrane receptors involved in cell adhesion and recognition in a variety of processes including embryogenesis, hemostasis, tissue repair, immune response and metastatic diffusion of tumor cells. This gene encodes a beta subunit. Multiple alternatively spliced transcript variants which encode different protein isoforms have been found for this gene. [provided by RefSeq, Jul 2008 ...
2015 Project header}} =,font color=red>Integrins,/font>= Integrins are a group of transmembrane cell adhesion proteins which anchor the cell to the extracellular matrix (ECM) by its cytoskeleton. Integrins not only anchor the cell, they are also involved in cell to cell adhesion,ref name=reffff>Alberts B, Johnson A, Lewis J, et al. Molecular Biology of the Cell. 4th edition. New York: Garland Science; 2002. Integrins. Available from: http://www.ncbi.nlm.nih.gov/books/NBK26867/,/ref>. However, integrins are not just adhesion proteins, as they can also induce intracellular signalling pathways in the extracellular matrix, which makes them play an important role in development, immune response, leukocyte trafficking and haemostasis. [[File:Integrin anchoring cytoskeleton to ecmII.png,thumb,350px,Figure 1:A simple diagram of how integrins interact with other proteins to anchor the cytoskeleton to the extracellular matrix]] The integrin family is one of the most highly studied cell adhesion ...
Although ACE2 (angiotensin converting enzyme 2) is considered the primary receptor for CoV-2 cell entry, recent reports suggest that alternative pathways may contribute. This paper considers the hypothesis that viral binding to cell-surface integrins may contribute to the high infectivity and widespread extra-pulmonary impacts of the SARS-CoV-2 virus. This potential is suggested on the basis of the emergence of an RGD (arginine-glycine-aspartate) sequence in the receptor-binding domain of the spike protein. RGD is a motif commonly used by viruses to bind cell-surface integrins. Numerous signaling pathways are mediated by integrins and virion binding could lead to dysregulation of these pathways, with consequent tissue damage. Integrins on the surfaces of pneumocytes, endothelial cells and platelets may be vulnerable to CoV-2 virion binding. For instance, binding of intact virions to integrins on alveolar cells could enhance viral entry. Binding of virions to integrins on endothelial cells could ...
Integrins are heterodimeric integral membrane proteins consisting of an α chain and a ß chain. It recognizes p1 and p2 peptides of fibrinogen gamma chain.
Integrins are cell adhesion molecules that play critical roles in development, wound healing, hemostasis, immunity and cancer. Advances in the past two years have shed light on the structural basis for integrin regulation and signaling, especially on how global conformational changes between bent an …
Monoclonal antibodies have become essential tools to study the structure and function of integrins because they recognise distinct conformational states of the receptors (Mould, 1996). Many of these antibodies have been used to study changes in the ligand-binding affinity of integrins on the cell surface (Humphries, 2000); however, in this study, we used their specificities to report defined conformational states during β1-integrin biosynthesis. In particular, we noted that two conformation-specific antibodies, 8E3 and 9EG7, which have been shown to recognise the unbent form of β1-integrins, also react with monomeric β1-integrin subunits. 9EG7 was of particular interest because it seems to recognise an epitope that requires the formation of a disulphide and once this disulphide is formed, the epitope is not lost even after denaturation. The epitope has been mapped previously to within a cysteine-rich stretch (residues 495-602) at the back of the β1-integrin knee region (Bazzoni et al., ...
Role of RGD-binding integrins in uPAR-induced protrusions and adhesion. (A) Swiss 3T3 cells were injected with pRc/CMV-uPAR (100 μg/ml) and incubated in growth
FITC偶联Integrin alpha 1抗体[TS2/7](ab34176)可与人样本反应并经Flow Cyt实验严格验证,被4篇文献引用,实验条件参看说明书。中国75%以上现货。
An integrin alpha subunit that primarily associates with INTEGRIN BETA1 or INTEGRIN BETA4 to form laminin-binding heterodimers. Integrin alpha6 has two alternatively spliced isoforms: integrin alpha6A and integrin alpha6B, which differ in their cytoplasmic domains and are regulated in a tissue-specific and developmental stage-specific manner ...
The extracellular matrix (ECM) is an insoluble network of proteins that provides structural support to nearly all multicellular tissues and organs as well as solid malignancies (1). Most metazoan cells dynamically interact with ECM components via integrins, which are heterodimeric transmembrane proteins composed of α and β subunits (2). Most integrins expressed on the cell surface are present in inactive conformations, and their adhesion to ECM ligands must be precisely regulated via inside-out activation mechanisms. Such regulatory mechanisms occur after extracellular stimuli (e.g., growth factors or cytokines), alter intracellular effector proteins that in turn bind to integrin cytoplasmic regions, and induce conformational changes in the integrin extracellular domains (2). Following activation and engagement with ECM ligands, integrins regulate cytoskeletal dynamics as well as intracellular signal transduction cascades that lead to a wide variety of cellular responses, including ...
The integrin family of cell surface receptors is evolutionary conserved and found in all multicellular animals. In humans 8-alpha and 18-beta integrins are non-covalently associated into 24 dimers. Integrins mediate cell-extracellular matrix and cell-cell interactions and participate in cell signalling. This ideally places integrins to regulate vital processes such as cell adhesion, migration, differentiation and cytoskeleton dynamics. Integrins also play a fundamental role in regulating cell survival and anoikis. In this thesis molecular mechanisms employed by integrins to induce signal transduction, independently or through crosstalk with other receptors, were characterised.. Rictor-mTOR (mTORC2) was required for Akt Ser473 phosphorylation in response to β1 integrin-mediated adhesion as well as EGF-, PDGF- or LPA-stimulation of MCF7 cells. ILK and PAK were dispensable for Akt Ser473 phosphorylation upon β1 integrin-engagement or EGF-stimulation. PAK was needed when this phosphorylation was ...
E7820 is an orally active inhibitor of α2-integrin mRNA expression currently Clec1b tested in phases I and II. transplanted into 7-week old female nude mice subcutaneously. Administration of E7820 was began at dosages of 12.5 25 50 100 PHA-739358 or 200?automobile or mg/kg PHA-739358 1 following the transplantation. E7820 was administered by gavage twice per day for 3 orally?weeks. PK examples had been gathered double weekly. Blood was withdrawn once a full week from the eye of anesthesized mice in PBS containing 0.004% sodium citrate and diluted at 1:100. Diluted bloodstream samples were straight stained with FITC-conjugated anti-integrin α2 Ab and appearance amounts on platelets had been analyzed by stream cytometry. The longest size from the tumor and bodyweight was measured double weekly by direct dimension from the tumor diameters with calipers. Software program The statistical data simulations and evaluation were performed with non-linear mixed-effects modeling using NONMEM version VI ...
Integrin alpha M (ITGAM) Antibody is a Rabbit Polyclonal antibody against Integrin alpha M (ITGAM). Integrin alpha M (ITGAM), also named as CD11B and CR3A, belongs to the integrin alpha chain family. It is implicated in various adhesive interactions of mo
The molecular structure of mouse Mucin 21 (Muc21)/epiglycanin is proposed to have 98 tandem repeats of 15 amino acids and three exceptional repeats with 12 or 13 amino acids each, followed by a stem domain, a transmembrane domain, and a cytoplasmic tail. A cDNA of Muc21 having 84 tandem repeats of 15 amino acids was constructed and transfected using a Venus vector into HEK 293T cells. The fluorescent cells, which were considered to express Muc21, were nonadherent. This antiadhesion effect was lessened when constructs with smaller numbers of tandem repeats were used, suggesting that the tandem repeat domain plays a crucial role. Cells expressing Muc21 were significantly less adherent to each other and to extracellular matrix components than control cells. Antibody binding to the cell surface integrin subunits alpha5, alpha6, and beta1 was reduced in Muc21 transfectants in a tandem repeat-dependent manner, whereas equal amounts of proteins were detected by Western blot analysis. Muc21 was ...
In their roles as major adhesion receptors, integrins signal across the plasma membrane in both directions. Recent structural and cell biological data suggest models for how integrins transmit signals between their extracellular ligand binding adhesion sites and their cytoplasmic domains, which link …
The ability of cells to migrate is a fundamental physiological process involved in embryonic development, tissue homeostasis, immune surveillance and wound healing. In order for cells to migrate, they must interact with their environment using adhesion receptors, such as integrins, and form specialized adhesion complexes that mediate responses to different extracellular cues. In this review, we discuss the role of integrin adhesion complexes (IACs) in cell migration, highlighting the layers of regulation that are involved, including intracellular signalling cascades, mechanosensing and reciprocal feedback to the extracellular environment. We also discuss the role of IACs in extracellular matrix remodeling and how they impact upon cell migration. ...
Integrins interact with extracellular matrix (ECM) and deliver intracellular signaling for cell proliferation, survival, and motility. During tumor metastasis, integrin-mediated cell adhesion to and migration on the ECM proteins are required for cancer cell survival and adaptation to the new microenvironment. Using stable isotope labeling by amino acids in cell culture-mass spectrometry, we profiled the phosphoproteomic changes induced by the interactions of cell integrins with type I collagen, the most common ECM substratum. Integrin-ECM interactions modulate phosphorylation of 517 serine, threonine, or tyrosine residues in 513 peptides, corresponding to 357 proteins. Among these proteins, 33 key signaling mediators with kinase or phosphatase activity were subjected to small interfering RNA-based functional screening. Three integrin-regulated kinases, DBF4, PAK2, and GRK6, were identified for their critical role in cell adhesion and migration possibly through their regulation of actin ...
マウス・モノクローナル抗体 ab24697 交差種: Hu 適用: IP,Neut,Flow Cyt,Inhibition,BL,ICC/IF…Integrin alpha 2+beta 1抗体一覧…画像、プロトコール、文献などWeb上の情報が満載のアブカムの…
TY - JOUR. T1 - The ligand-induced conformational change of α5β1 integrin. Relocation of α5 subunit to uncover the β1 stalk region. AU - Tsuchida, Jun. AU - Ueki, Shoko. AU - Takada, Yoshikazu. AU - Saito, Yuji. AU - Takagi, Junichi. PY - 1998. Y1 - 1998. N2 - Integrin heterodimers undergo a conformational change upon the binding of ligand to their extracellular domains. An anti-β1 integrin monoclonal antibody AG89 can detect such a conformational change since it recognizes a ligand-inducible epitope in the stalk-like region of β1 subunits. The binding of a 125I-labeled AG89 Fab fragment to α5β1 integrins on K562 cells was assessed and analyzed by the Scatchard method. High affinity binding sites for AG89 are present on cells treated with ligand peptide. In addition, results revealed that cells treated with EDTA also express AG89 binding sites with the same affinity although the number of binding sites is 4-fold lower. AG89 immunoprecipitated α5β1 complexes from surface-labeled K562 ...
Sato T, Ohashi Y, Tachibana K, Soiffer RJ, Ritz J, Morimoto C. Altered tyrosine phosphorylation via the very late antigen (VLA)/beta1 integrin stimulation is associated with impaired T-cell signaling through VLA-4 after allogeneic bone marrow transplantation. Blood. 1997 Nov 15; 90(10):4222-9 ...
Integrin alpha 5 antibody [HM alpha 5] (integrin alpha 5 (fibronectin receptor alpha)) for FACS, IP, WB. Anti-Integrin alpha 5 mAb (GTX41950) is tested in Mouse, Rat samples. 100% Ab-Assurance.
The polymorphisms C807T end G873A of the platelet integrin alpha2b ta1 collagen receptor glycoprotein GP Ia-IIa are linked to the expression density of this receptor. The GPIa T807/A873 allele causes a higher receptor expression, enhancing platelet binding to collagen. This might present a genetic predisposition for the development of...
The cell adhesion molecule integrin alpha(v)beta(3) is an important player in the process of tumor angiogenesis and metastasis. Abegrin (TM), a fully humanized anti-integrin alpha(v)beta(3) monoclonal antibody, was currently in clinical trials for cancer therapy. Herein, we labeled Abegrin (TM) with In-111, evaluated the in vitro and in vivo characteristics, and investigated whether the expression of integrin alpha(v)beta(3) in tumors could be imaged with In-111-labeled Abegrin (TM).. The binding affinity and specificity of Abegrin (TM) was analyzed using U87MG glioblastoma cells. Abegrin (TM) was coupled with 1,4,7,10-tetraazadodecane-N,N′,NaEuro(3),N′aEuro(3)-tetraacetic acid (DOTA) for In-111 radiolabeling. gamma Imaging of In-111-DOTA-Abegrin (TM) was carried out in nude mice bearing both integrin alpha(v)beta(3)-positive U87MG and integrin alpha(v)beta(3)-negative HT-29 tumors. Biodistribution and blocking studies of In-111-DOTA-Abegrin (TM) were investigated in U87MG tumor-bearing nude ...
Integrins are cell surface receptors that physically bridge the extracellular matrix to the cytoskeleton and responsible for adhesion, migration, and signaling. Integrin function is intimately controlled by their membrane traffic. For example, integrins are dynamically internalized from the cell posterior and recycled to the cell anterior during cell migration. Misregulation of integrins is intimately linked with cancer progression, including metastasis and cell proliferation and survival. We have recently uncovered that integrin membrane traffic is controlled by AMP-activated protein kinase (AMPK), an energy stress sensing kinase within cells at becomes activated upon energy stress such as by an increase in cell AMP:ATP ratio. I confirmed that AMPK activation resulted in a reduction of cell surface β1-integrin. Using assays that selectively measure integrin exocytosis and endocytosis, I found that AMPK activation regulates β1-integrin recycling and possibly endocytosis. I demonstrated that ...
OBJECTIVE: It was recently demonstrated that synoviocytes (FLS) from rheumatoid arthritis (RA) patients express BAFF transcripts that are up-regulated by tumor necrosis factor alpha (TNFalpha) and interferon-gamma (IFNgamma). Thus, BAFF increases in RA target cells might be related to activation of the receptors of innate immunity. The purpose of this study was to determine whether ligands of Toll-like receptor 2 (TLR-2), TLR-4, TLR-9, and alpha5beta1 integrin are able to induce BAFF synthesis by RA FLS. METHODS: Quantitative reverse transcription-polymerase chain reaction analyses and enzyme-linked immunosorbent assays were performed to evaluate BAFF messenger RNA induction and BAFF release from FLS after stimulation by ligands for TLR-2, TLR-4, TLR-9, alpha5beta1 integrin (bacterial lipopeptide [BLP] palmitoyl-3-cysteine-serine-lysine-4, lipopolysaccharide [LPS], CpG, and protein I/II, respectively), TNFalpha, and IFNgamma. RESULTS: In contrast to IFNgamma, neither TNFalpha, LPS, BLP, nor CpG induced
During cell migration, integrins are redistributed from focal adhesions undergoing disassembly in the cells walking sides to brand-new focal adhesions putting together in leading sides. the walking sides and the set up of brand-new focal adhesions at the migrating methodologies (Lauffenburger and Horwitz, 1996; Caswell et al., 2009). Constitutive integrin turnover, internalization, and taking have got been confirmed under basal cell migration circumstances (Pellinen and Ivaska, 2006; Mosesson et al., 2008). In latest years, clathrin-mediated endocytosis provides been proven to play a pivotal function in the internalization of surface area integrins at focal adhesions that are going through basal Troxacitabine turnover (Chao and Kunz, 2009; Ezratty et al., 2009). Nevertheless, few research have got analyzed powerful integrin disassembly, redistribution, and reassembly in extremely motile cells (Webb et al., 2002). In reality, in vivo cell migration is certainly often significantly increased by ...
Binding studies of purified, platelet α2β1 integrin, and of ligand-binding A domain of this integrin to native collagen and to purified 3/4 and 1/4 fragments of collagen, generated by collagenase-3 revealed the necessity for retention of the triple-helical conformation of the collagen fragments for α2β1 integrin binding. HT1080 cell attachment to type I collagen was α2β1 integrin-mediated, and collagenase-cleavage of type I collagen perturbed cell attachment via this integrin at physiological temperature. This is likely to be due to melting of the collagen fragments, destabilizing of the triple-helical conformation of type I collagen and the resultant loss of α2β1 integrin binding sites on the collagen molecules. However, cell attachment to the native collagen fragments was still possible at room temperature, indicating that collagenase does not destroy α2β1 integrin binding sites on type I collagen, and that attachment can occur below physiological temperature ...
Given its effects on cell spreading and motility, Rap1 was postulated some time ago to be involved in integrin function; however, confirmation of this required alternative cellular models. Leukocytes represented such a model, since the positive effect of GTP-bound Rap1 on integrin-mediated adhesion is easier to see: in blood cells, in contrast to the more traditional, fibroblast and epithelial, adherent cell systems, integrins are normally kept inactive. Upon inside-out signalling elicited by various agonists, leukocyte integrins can rapidly and transiently be converted to a functionally active, ligand-binding state able to trigger the classic outside-in signalling to Rho-like GTPases (Harris et al., 2000; Schoenwaelder and Burridge, 1999).. Rap1 regulates functional activation of several integrin heterodimers:α 4β1 (VLA-4), α5β1 (VLA-5), αLβ2 (LFA-1, CD11a/CD18), αMβ2 (CR3, CD11b/CD18) and αIIbβ3 (Reedquist et al., 2000; Caron et al., 2000; Katagiri et al., 2000; Arai et al., 2001; ...
Rabbit Monoclonal Anti-Integrin alpha V beta 3 Antibody (SC56-07). Validated: WB, Flow, ICC/IF. Tested Reactivity: Human, Mouse, Rat. 100% Guaranteed.
aß heterodimeric receptors (4). Recently, two types of signaling by integrins have been extensively discussed: transmission of signals into the cell following binding of ligands or counter-receptors to the integrins (outside-in signaling), and regulation of the avidity and conformation of integrins by signals generated by other receptors within the cell (inside-out signaling) (5). Interaction of integrins with the ECM proteins can induce tyrosine phosphorylation of many intracellular proteins.. Integrin clusters in macromolecular complexes, ligand occupancy, and tyrosine phosphorylation are the key events that result in diverse processes such as cell migration and differentiation, tissue remodelling, cell proliferation, angiogenesis, and tumor cell invasion and metastasis (3,6). Focal adhesion kinase (FAK) becomes tyrosine-phosphorylated during integrin-mediated cell adhesion and is believed to play important roles in integrin signal transduction (7,8). FAK, a non-receptor tyrosine kinase, ...
Abstract Background The regulation of normal and stress-induced erythropoiesis is incompletely understood. Integrin-dependent adhesion plays important roles in erythropoiesis, but how integrins are regulated during erythropoiesis remains largely elusive. Design and Methods To obtain novel insights into the regulation of erythropoiesis, we used cellular and molecular approaches to analyze the role of SWAP-70 and the control of integrins through SWAP-70. In addition, mice deficient for this protein were investigated under normal and erythropoietic stress conditions. Results We show that SWAP-70, a protein involved in cytoskeletal F-actin rearrangements and integrin regulation in mast cells, is expressed in hematopoietic stem cells and myeloid-erythroid precursors. Although Swap-70-/- mice are not anemic, erythroblastic differentiation is perturbed, and SWAP-70 is required for an efficient erythropoietic stress response to acute anemia and for erythropoietic recovery after bone marrow ...
The major contribution of β2 integrins in immune defense and inflammatory processes relates to their pivotal role in mediating cellular contacts between leukocytes and endothelium as a prerequisite for subsequent transmigration towards a chemotactic stimulus. Previous in vitro studies have demonstrated that uPAR forms complexes with integrins ((12), (13)) and thereby modulates integrin-mediated binding to extracellular matrix proteins ((26)-(28)).. This study demonstrates that uPAR is needed for β2 integrin-dependent leukocyte recruitment into sites of acute inflammation. Migration of neutrophils and monocytes into the inflamed peritoneum was drastically reduced after 4 h in uPAR-deficient mice. Consistently, β2 integrin- dependent cell adhesion of leukocytes to endothelial cells was abrogated after depletion of uPAR from the cell surface, whereas reconstitution with soluble intact, but not truncated, uPAR could totally rescue β2 integrin-mediated adhesion. Regulation of β2 integrin ...
This study demonstrates an important role for the nonreceptor tyrosine kinase Pyk2 in the integrin-mediated activation of PMNs that contributes to normal degranulation responses required for efficient host defense to S. aureus infection. Pyk2-deficient PMNs exhibited reduced degranulation responses following integrin ligation both in vitro and during bacterial infection in vivo; however, they responded normally to soluble agonists, suggesting that the integrin signaling pathway was the major response affected in the pyk2 mutant cells. It is clear that unlike Src-family or Syk tyrosine kinases, Pyk2 is acting in a more distal step in the integrin signaling pathway, because many integrin-mediated functions were normal in pyk2−/− PMNs, including attachment, adhesion, and integrin-mediated activation of superoxide production. These limited impairments correlate with the only partially reduced integrin-mediated tyrosine phosphorylation responses, although reduction in phosphorylation of specific ...
This paper shows that, in confluent human umbilical vein endothelial cell (EC) monolayers, the integrin heterodimers alpha 2 beta 1 and alpha 5 beta 1, but not other members of the beta 1 subfamily, are located at cell-cell contact borders and not at cellular free edges. Also the alpha v chain, but not its most common partner beta 3, that is widely expressed in EC cell-matrix junctions, is found at cell-cell borders. In EC monolayers, the putative ligands of alpha 2 beta 1 and alpha 5 beta 1 receptors, i.e., laminin, collagen type IV, and fibronectin, are also organized in strands corresponding to cell-cell borders. The location of the above integrin receptors is not an artifact of in vitro culture since it has been noted also in explanted islets of the native umbilical vein endothelium. The integrins alpha 2 beta 1 and alpha 5 beta 1 play a role in the maintenance of endothelial monolayer continuity in vitro. Indeed, specific antibodies to alpha 2 beta 1, alpha 5 beta 1, and the synthetic ...
Article: Targeting myeloid-cell specific integrin α9β1 inhibits arterial thrombosis in mice. Authors: Nirav Dhanesha, Manasa K. Nayak, Prakash Doddapattar, Manish Jain, Gagan D. Flora, Shigeyuki Kon, Anil K. Chauhan Journal: Blood. 2020 Mar 12;135(11):857-861. doi: 10.1182/blood.2019002846. Abstract:Evidence suggests that neutrophils contribute to thrombosis via several mechanisms, including neutrophil extracellular traps (NETs) formation. Integrin α9β1 is…
TY - JOUR. T1 - αvβ3 and αvβ5 integrin expression in glioma periphery. AU - Bello, Lorenzo. AU - Francolini, Maura. AU - Marthyn, Paola. AU - Zhang, Jianping. AU - Carroll, Rona S.. AU - Nikas, Demetrios C.. AU - Strasser, Jon F.. AU - Villani, Roberto. AU - Cheresh, David A.. AU - McL. Black, Peter. PY - 2001. Y1 - 2001. N2 - OBJECTIVE: This study analyzed the expression of integrins αvβ3 and αvβ5 in glioma tissue and focused on the periphery of high-grade gliomas. METHODS: The analysis was performed with Western blot, immunohistochemistry, and immunofluorescence, by use of two monoclonal antibodies able to recognize the functional integrin heterodimer. The expression of integrin-related ligands and growth factors also was studied. Sections from the tumor periphery were classified as either tumor periphery (light tumor infiltrate or scant visible cells) or peritumor (heavy tumor infiltration). RESULTS: Our data on glioma tissues demonstrated that both integrins were expressed in glioma ...
Peptides or short protein domains derived from the extracellular matrix are reported to possess anti-tumor properties. In the present study, one such molecule Hexastatin or [α6(IV)NC1] a non-collagenous domain of α6 chain of type (IV) collagen was derived from the carboxy terminal and was found to inhibit tumor growth, but the mechanism by which it inhibits the growth of solid tumors has not been reported yet. In the present study, we identified that the biological functions of hexastatin are attributed to its binding to different cell surface integrins. We identified that hexastatin binds to α3β1, αVβ3 and α5β1 integrins and inhibits p38-MAPK signaling. Hexastatin competes with human vascular endothelial cells in binding to α1β1 integrins on type IV collagen, and or with α5β1/αVβ3 integrins on fibronectin/vitronectin matrix, thus inhibiting endothelial migration and tube formation. Interestingly, p38-MAPK phosphorylation was not inhibited in α3 and β3 integrin null endothelial ...
Fibronectin (FN) is an abundant component of the ECM expressed during embryonic development, angiogenesis, wound healing, and tumor invasion. Cell migration is critical for all these conditions and the motile cells often display altered expression of FN-binding integrins such as α5β1, αvβ3, and others (Mizejewski, 1999; Stupack and Cheresh, 2002; Watt, 2002). All these integrins bind the RGD motif in the central cell-binding domain of FN (Pankov and Yamada, 2002), but it is still unclear if and how they mediate specific cellular responses to FN. Here, we explored the possibility that they promote different aspects of cell migration and that switching integrins allows cells to modulate their motile response to FN.. The dynamic regulation of the F-actin network is crucial to cell migration and is mediated by multiple actin-polymerizing, -capping, -severing, and -cross-linking proteins (Pollard and Borisy, 2003; Ridley et al., 2003). The Arp2/3 complex and cofilin are involved in the generation ...
Integrins alpha5beta1, alphavbeta1, and alphavbeta6 collaborate in squamous carcinoma cell spreading and migration on fibronectin is an eagle-i resource of type Journal article at eagle-i Network Shared Resource Repository.
Integrin beta-3 (β3) or CD61 is a protein that in humans is encoded by the ITGB3 gene. CD61 is a cluster of differentiation found on thrombocytes. The ITGB3 protein product is the integrin beta chain beta 3. Integrins are integral cell-surface proteins composed of an alpha chain and a beta chain. A given chain may combine with multiple partners resulting in different integrins. Integrin beta 3 is found along with the alpha IIb chain in platelets. Integrins are known to participate in cell adhesion as well as cell-surface-mediated signaling. Defectively expressed β3 integrin subunit has been correlated with presence of endometriosis, and has been suggested as a putative marker of this condition. CD61 has been shown to interact with PTK2, ITGB3BP, TLN1 and CIB1. Glycoprotein IIb/IIIa GRCh38: Ensembl release 89: ENSG00000259207 - Ensembl, May 2017 GRCm38: Ensembl release 89: ENSMUSG00000020689 - Ensembl, May 2017 Human PubMed Reference:. Mouse PubMed Reference:. Sosnoski DM, Emanuel BS, ...
JAK2-V617F-positive chronic myeloproliferative neoplasia (CMN) commonly displays dysfunction of integrins and adhesion molecules expressed on platelets, erythrocytes, and leukocytes. However, the mechanism by which the 2 major leukocyte integrin chains, β1 and β2, may contribute to CMN pathophysiology remained unclear. β1 (α4β1; VLA-4) and β2 (αLβ2; LFA-1) integrins are essential regulators for attachment of leukocytes to endothelial cells. We here showed enhanced adhesion of granulocytes from mice with JAK2-V617F knockin (JAK2+/VF mice) to vascular cell adhesion molecule 1- (VCAM1-) and intercellular adhesion molecule 1-coated (ICAM1-coated) surfaces. Soluble VCAM1 and ICAM1 ligand binding assays revealed increased affinity of β1 and β2 integrins for their respective ligands. For β1 integrins, this correlated with a structural change from the low- to the high-affinity conformation induced by JAK2-V617F. JAK2-V617F triggered constitutive activation of the integrin inside-out signaling ...
JAK2-V617F-positive chronic myeloproliferative neoplasia (CMN) commonly displays dysfunction of integrins and adhesion molecules expressed on platelets, erythrocytes, and leukocytes. However, the mechanism by which the 2 major leukocyte integrin chains, β1 and β2, may contribute to CMN pathophysiology remained unclear. β1 (α4β1; VLA-4) and β2 (αLβ2; LFA-1) integrins are essential regulators for attachment of leukocytes to endothelial cells. We here showed enhanced adhesion of granulocytes from mice with JAK2-V617F knockin (JAK2+/VF mice) to vascular cell adhesion molecule 1- (VCAM1-) and intercellular adhesion molecule 1-coated (ICAM1-coated) surfaces. Soluble VCAM1 and ICAM1 ligand binding assays revealed increased affinity of β1 and β2 integrins for their respective ligands. For β1 integrins, this correlated with a structural change from the low- to the high-affinity conformation induced by JAK2-V617F. JAK2-V617F triggered constitutive activation of the integrin inside-out signaling ...
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CD49d / integrin alpha 4, unlike other alpha integrins, neither contains an I-domain, nor undergoes disulfide-linked cleavage. It associates with beta 7 chain to form alpha 4 / beta 7 integrin, and with beta 1 chain (CD29) to form VLA-4 integrin. These complexes are important for lymphocyte migration from circulation into tissue (binding VCAM-1) and homing of T cell subsets to Peyer s patches (binding MadCAM-1), but VLA-4 is also target for invasive bacteria which contain invasin. CD49d is essential for differentiation and migration of hematopoietic stem cells by their adhesion to bone marrow stromal cells, and provides a costimulatory signal to TCR-CD3 complex by inducing phosphorylation of some focal adhesion proteins ...
Integrins are a family of cell adhesion molecules comprising 18α and 8β subunits that combine into at least 24 heterodimers. Intβ1 partners with α subunits to form 12 potential integrin receptors, which bind to a wide array of arginine-glycine-aspartic acid (RGD)-containing extracellular matrix (ECM) molecules such as collagens, laminin, and fibronectin (17, 18). Intβ1 consists of a large extracellular domain, a single transmembrane stretch, and a short cytoplasmic domain devoid of an intrinsic enzymatic activity. The cytoplasmic domain transduces bidirectional signals from inside the cell by regulating the conformation and ligand affinities of the extracellular domain of Intβ1 (inside-out signaling), while mediating downstream signaling and interactions with the cytoskeleton (outside-in signaling). The outside-in signaling is initiated upon ligand binding to Intβ1, followed by the formation of adhesion complexes assembled from signaling molecules, such as tyrosine kinases, ...
This gene encodes an RGD-containing protein that binds to type I, II and IV collagens. The RGD motif is found in many extracellular matrix proteins modulating cell adhesion and serves as a ligand recognition sequence for several integrins. This protein plays a role in cell-collagen interactions and may be involved in endochondrial bone formation in cartilage. The protein is induced by transforming growth factor-beta and acts to inhibit cell adhesion. Mutations in this gene are associated with multiple types of corneal dystrophy. [provided by RefSeq, Jul 2008 ...
TY - JOUR. T1 - Cardiac integrins. T2 - The ties that bind. AU - Simpson, David G.. AU - Reaves, Titus A.. AU - Shih, Daw Tsun. AU - Burgess, William. AU - Borg, Thomas K.. AU - Terracio, Louis. PY - 1998/5. Y1 - 1998/5. N2 - An elaborate series of morphogenetic events must be precisely coordinated during development to promote the formation of the elaborate three-dimensional structure of the normal heart. In this study we focus on discussing how interconnections between the cardiac myocyte and its surrounding environment regulate cardiac form and function. In vitro experiments from our laboratories provide direct evidence that cardiac cell shape is regulated by a dynamic interaction between constituents of the extracellular matrix (ECM) and by specific members of the integrin family of matrix receptors. Our data indicates that phenotypic information is stored in the tertiary structure and chemical identity of the ECM. This information appears to be actively communicated and transduced by the α ...
The signaling events downstream of integrins that regulate cell attachment and motility are only partially understood. Finally, we show that deletion of c Src or c-Cbl qualified prospects to a lower in osteoclast migration. Therefore, presenting of sixth is v3 integrin induce the development of a Pyk2/Src/Cbl complicated in which Cbl can be a crucial regulator of Src kinase activity and of cell adhesion and migration. These findings might explain the osteopetrotic phenotype in the Src?/? rodents. check. CENPA Transient and Steady Transfections Cells were transfected for 5 h using LipofectAMINE? (Existence Systems) in -MEM pursuing the manufacturers process. Steady 293-VnR cell lines articulating myc-tagged Cbl constructs had been founded by moving transfected cells into tradition moderate supplemented with 100 g/ml G418 (Existence Systems) and 200 g/ml Zeocin (Invitrogen) 72 l after transfection. Cells had been taken care of in this moderate until resistant colonies of cells had been shaped (2 ...
CD31 is a member of the immunoglobulin superfamily consisting of six Ig-related domains. It is constitutively expressed by platelets, monocytes, and some lymphocytes, but at tenfold higher levels on vascular endothelial cells. CD31 has both homotypic and heterotypic adhesive properties. We have mapped the homotypic binding sites using a deletion series of CD31-Fc chimeras and a panel of anti-CD31 monoclonal antibodies. An extensive surface of CD31 is involved in homotypic binding with domains 2 and 3 and domains 5 and 6 playing key roles. A model consistent with the experimental data is that CD31 on one cell binds to CD31 on an apposing cell in an antiparallel interdigitating mode requiring full alignment of the six domains of each molecule. In addition to establishing intercellular homotypic contacts. CD31 binding leads to augmented adhesion via beta 1 integrins. The positive cooperation between CD31 and beta 1 integrins can occur in heterologous primate cells (COS cells). The interaction is ...
Integrins are proteins important for integrating signals from cell to cell and for interpreting cues from their microenvironment that greatly influencing cell b...
The research focus of Rolf Reeds group is on the how the extracellular matrix participates in determining the properties of the tumors and how this in turn is reflected in growth and metastasis. Additional foci have been on how collagen binding integrins influence these properties and also how tumor hypoxia does the same.
Human Integrin beta 1 peptide Peptide datasheet (ab105996). Abcam offers quality products including antibodies, assays and other reagents.
This image shows the perineurial glia that ensheath the nervous system in Drosophila. Perlecan tagged with GFP (green) marks the extracellular matrix that tightly associates with the perineurial glia (labeled with a red, RFP-tagged membrane marker). The perineurial glia in turn ensheath the entire peripheral nerve including the peripheral axons (labeled in blue) at the core of each nerve. The transmembrane protein Basigin interacts with integrins at focal adhesions to regulate the interaction of integrins with the extracellular matrix, and loss of Basigin leads to compression of the peripheral nerve. For more information, see the article by Hunter et al. (pages 3360-3373). ...
Background Integrin signaling is an attractive target for anti-cancer treatment. GLPG0187 is a broad spectrum integrin receptor antagonist (IRA). GLPG0187 inhibited tumor growth and metastasis in mouse models. Methods We aimed to determine the Recommended Phase II Dose (RP2D) and to assess safety and tolerability of continuous i.v. infusion in ... read more patients with advanced malignant solid tumors. Anticipated dose levels were 20, 40, 80, 160, 320, and 400 mg/day in a modified 3 + 3 design. Plasma concentrations of GLPG0187 were assessed to characterize the pharmacokinetics (PK). C-terminal telopeptide of type I collagen (CTX) was used as pharmacodynamics marker. Results Twenty patients received GLPG0187. No dose limiting toxicities (DLTs) were observed. The highest possible and tested dose was 400 mg/day. Fatigue was the most frequently reported side effect (25 %). Recurrent Port-A-Cath-related infections and skin toxicity suggest cutaneous integrin inhibition. No dose-dependent toxicity ...
Disparities are observed in the expression pattern of the RXRs. RXRβ is widely distributed and can be detected in almost every tissue (Hamada et al., 1989; Yu et al., 1991; Mangelsdorf et al., 1992; Dolle et al., 1994). RXRα is predominantly expressed in liver, kidney, epidermis, and intestine and is the major RXR in skin (Mangelsdorf et al., 1990, 1992; Dolle et al., 1994). RXRγ is mostly restricted to the muscle and certain parts of the brain as well as to the pituitary (Mangelsdorf et al., 1992; Dolle et al., 1994; Haugen et al., 1997; Chiang et al., 1998).. In addition to the fact that RXRs are heterodimeric partners of multiple nuclear receptors regulating various developmental and metabolic processes, this RXR distribution suggests that RXRs play critical roles in a wide range of these processes. To address the issue of the RXR functional role in vivo, knockout of all three RXRs has been performed in the mouse (for comprehensive reviews, see Kastner et al., 1995; Mark and Chambon, 2003; ...
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Krammer, A.; Craig, D.; Thomas, W.E.; Schulten, K.; Vogel, V., 2002: A structural model for force regulated integrin binding to fibronectin's RGD-synergy site
Integrins[edit]. Integrins involved in cellular adhesion are primarily expressed on leukocytes. β2 integrins on rolling ... Integrin molecules migrate to the cell surface and congregate in high-avidity patches. Intracellular integrin domains associate ... Integrin ligands ICAM-1 and VCAM-1 are activated by inflammatory cytokines, while ICAM-2 is constitutively expressed by some ... During chemotaxis, cell movement is facilitated by the binding of β1 integrins to components of the extracellular matrix: VLA-3 ...
Estevez, Brian; Shen, Bo; Du, Xiaoping (January 23, 2015). "Targeting Integrin and Integrin Signaling in Treating Thrombosis". ... His studies have focused on identifying mechanisms whereby β3 integrins mediate responses to thrombin and other SMC agonists. ... Sajid, M.; Zhao, R.; Pathak, A.; Smyth, S. S.; Stouffer, G. A. (November 23, 2003). "Alphavbeta3-integrin antagonists inhibit ... Stouffer, G. A.; Smyth, S. S. (November 1, 2003). "Effects of thrombin on interactions between beta3-integrins and ...
Integrins link extracellular matrix to keratin intermediate filaments, which interacts with intracellular domain of integrins ... Cell-matrix junctions are mainly mediated by integrins, which also clusters like cadherins to form firm adhesions. Integrins ... can induce conformational changes in integrins and initiate signalling cascades. Extracellular domains of integrins can bind to ... At these sites, integrins on the rolling white blood cells are activated and bind firmly to the local endothelial cells, ...
Schwartz, MA; Ingber, DE (1994). "Integrating with integrins". Molecular Biology of the Cell. 5 (4): 389-93. doi:10.1091/mbc. ... PMID 2938015.CS1 maint: multiple names: authors list (link) Brakebusch, C; Fässler, R (2003). "The integrin-actin connection, ... bound to the cytoplasmic domains of integrins. He then discovered other focal adhesion components including paxillin and ...
Schnapp, L (2006). Integrin, Adhesion/cell-matrix. Seattle: Elsevier. García AJ (December 2005). "Get a grip: integrins in cell ... The integrins contain multiple divalent cation binding sites in the extracellular domain ). The integrin cation binding sites ... Integrins and the Ig-superfamily CAMs do not depend on Ca2+ while cadherins and selectins depend on Ca2+. In addition, ... Integrins are heterodimeric, as they consist of an alpha and beta subunit. There are currently 18 alpha subunits and 8 beta ...
When an integrin receptor binds to its ECM ligand and is activated, additional integrins cluster around the activated site. In ... and integrin receptors (of which there exist several types on chondrocytes). Using the integrin-linked mechanotransduction ... Integrins have been identified on the upper shaft of the cilium, acting as anchors to the collagen matrix around it. Recent ... Integrin signaling is just one example of multiple pathways that are activated when cartilage is loaded. Some intracellular ...
It may be mediated by interaction through CXCR4 (CD184) the receptor for CXC Chemokines (e.g., SDF1) and α4β1 integrins. ... and alpha4beta1 integrins mediate spontaneous migration of human CD34+ progenitors and acute myeloid leukaemia cells beneath ...
Fluciclatide, an 18F-labelled small peptide that binds to integrin αVβ3 and integrin αVβ5, is being tested as a tool to monitor ... an 18F-Labeled αVβ3-Integrin and αVβ5-Integrin Imaging Agent". Journal of Nuclear Medicine. 52 (3): 424-430. doi:10.2967/jnumed ... CEND-1, also known as iRGD, is a cyclic peptide that homes to tumors via binding to Integrin alpha V receptors. However, it ... Hynes, R (1987). "Integrins: A family of cell surface receptors". Cell. 48 (4): 549-554. doi:10.1016/0092-8674(87)90233-9. PMID ...
Its occurrence in collagen types VI, VII, XII and XIV, the integrins and other proteins by averaged structure predictions". J. ... the integrins (I-domains); collagen types VI, VII, XII and XIV; and other extracellular proteins. Although the majority of VWA- ...
Integrins lack kinase activity; hence, integrin-mediated signal transduction is achieved through a variety of intracellular ... In plants, there are no bona fide integrin receptors identified to date; nevertheless, several integrin-like proteins were ... integrins of circulating cells are normally inactive. For example, cell membrane integrins on circulating leukocytes are ... Integrins are produced by a wide variety of cells; they play a role in cell attachment to other cells and the extracellular ...
Leahy DJ, Qu A (1995). "Crystal structure of the I-domain from the CD11a/CD18 (LFA-1, alpha L beta 2) integrin". Proc. Natl. ... Lee JO, Rieu P, Arnaout MA, Liddington R (1995). "Crystal structure of the A domain from the alpha subunit of integrin CR3 ( ... Its occurrence in collagen types VI, VII, XII and XIV, the integrins and other proteins by averaged structure predictions". J. ... 3D structures have been determined for the I-domains of integrins CD11b (with bound magnesium) and CD11a (with bound manganese ...
It forms complexes with proteins called integrins. Integrins link cells together. The complex formation may inhibit detachment ...
Streuli, Charles H. (2009-01-15). "Integrins and cell-fate determination". Journal of Cell Science. 122 (2): 171-177. doi: ...
Integrins act as adhesion receptors, transporting signals across the plasma membrane in multiple directions. These molecules ... There are four main types: selectins, cadherins, integrins, and the immunoglobulin superfamily. Selectins are cell adhesion ... Hynes RO (September 2002). "Integrins: bidirectional, allosteric signaling machines". Cell. 110 (6): 673-87. doi:10.1016/S0092- ... their transmembrane anchors are composed of cadherins in those that anchor to other cells and integrins in those that anchor to ...
Belkin AM, Stepp MA (2000). "Integrins as receptors for laminins". Microsc. Res. Tech. 51 (3): 280-301. doi:10.1002/1097-0029( ...
"The integrin binding site 2 (IBS2) in the talin rod domain is essential for linking integrin beta subunits to the cytoskeleton ... "The Talin head domain binds to integrin beta subunit cytoplasmic tails and regulates integrin activation". The Journal of ... In addition to F-actin, the N-terminal region of talin-1 binds layilin, β1- and β3-integrin, and focal adhesion kinase. Talin-1 ... Wu JC, Sung HC, Chung TH, DePhilip RM (2002). "Role of N-cadherin- and integrin-based costameres in the development of rat ...
Cells use mainly the receptor integrin to interact with ECM proteins. This signaling can influence the cell cycle and cellular ... Giancotti, FG; Ruoslahti, E (Aug 13, 1999). "Integrin signaling". Science. 285 (5430): 1028-32. doi:10.1126/science.285.5430. ...
It binds to integrins of type CD11a / CD18, or CD11b / CD18 and is also exploited by rhinovirus as a receptor for entry into ... ICAM-1 is a ligand for LFA-1 (integrin), a receptor found on leukocytes. When activated, leukocytes bind to endothelial cells ... Yonekawa K, Harlan JM (Feb 2005). "Targeting leukocyte integrins in human diseases". Journal of Leukocyte Biology. 77 (2): 129- ... "Structures of the alpha L I domain and its complex with ICAM-1 reveal a shape-shifting pathway for integrin regulation". Cell. ...
Primarily, the VCAM-1 protein is an endothelial ligand for VLA-4 (Very Late Antigen-4 or integrin α4β1) of the β1 subfamily of ... Yonekawa K, Harlan JM (2005). "Targeting leukocyte integrins in human diseases". J. Leukoc. Biol. 77 (2): 129-40. doi:10.1189/ ... integrins. VCAM-1 expression has also been observed in other cell types (e.g., smooth muscle cells). It has also been shown to ...
Loeser RF (2002). "Integrins and cell signaling in chondrocytes". Biorheology. 39 (1-2): 119-24. PMID 12082274. Overview of all ... integrin-mediated signaling, cell adhesion, and osteoclast motility". J. Cell Biol. 152 (1): 181-95. doi:10.1083/jcb.152.1.181 ... in a cytoskeleton-dependent signaling pathway initiated by ligation of integrin or antigen receptor on human B cells". J. Biol ... "The related adhesion focal tyrosine kinase is tyrosine-phosphorylated after beta1-integrin stimulation in B cells and binds to ...
This provides a mechanism by which Rap1 can act directly on integrins and modulate integrin affinity and/or clustering. PKD, ... particularly the interaction of talin with integrin cytoplasmic tails. Given the known role of talin in regulating integrin ... This finding suggests that LFA-1 clustering is critical for TCR signaling to integrins that is mediated by Rap1. It also ... RAPL is also an integrin-associated protein as RAPL polarizes to the immunological synapse following antigen stimulation of T ...
It is presumed through a direct association with the cytoplasmic tail of beta-integrin. The N-terminal region of paxillin is ... Lewis JM, Schwartz MA (June 1998). "Integrins regulate the association and phosphorylation of paxillin by c-Abl". The Journal ... Rose DM, Han J, Ginsberg MH (August 2002). "Alpha4 integrins and the immune response". Immunological Reviews. 186: 118-24. doi: ... Nikolopoulos SN, Turner CE (June 2001). "Integrin-linked kinase (ILK) binding to paxillin LD1 motif regulates ILK localization ...
Bernstein CN, Sargent M, Gallatin WM (February 1998). "Beta2 integrin/ICAM expression in Crohn's disease". Clinical Immunology ... Yonekawa K, Harlan JM (February 2005). "Targeting leukocyte integrins in human diseases". Journal of Leukocyte Biology. 77 (2 ... Bernstein CN, Sargent M, Gallatin WM, Wilkins J (October 1996). "Beta 2-integrin/intercellular adhesion molecule (ICAM) ... "Crystal structure of ICAM-2 reveals a distinctive integrin recognition surface". Nature. 387 (6630): 312-5. Bibcode:1997Natur. ...
Integrin signalling Integrin αvβ3 (a cell-surface adhesion molecule) is important for tumor attachment, cell-to-cell ... Several different integrins bind to fibronectin. Fibronectin-integrin interactions are important in tumor cell migration, ... invasion, metastasis and cell proliferation by signaling through integrins. Integrin-mediated tumor cell adhesion to ECM ... Endoglin is a cell-surface disulfide-linked homodimeric glycoprotein which binds to integrins and other RGD ligands and is a co ...
Kühn, Klaus (1997). "Extracellular matrix constituents as integrin ligands". In Elbe, Johannes A. (ed.). Integrin-ligand ... In muscle, it binds to alpha dystroglycan and integrin alpha7-beta1 via the G domain, and via the other end it binds to the ... They also bind to cell membranes through integrin receptors and other plasma membrane molecules, such as the dystroglycan ... This leads to cell signalling through integrins and dystroglycan (and possibly other receptors) recruited to the adherent ...
Integrin, alpha E (ITGAE) also known as CD103 (cluster of differentiation 103) is an integrin protein that in human is encoded ... CD103 binds integrin beta 7 (β7- ITGB7) to form the complete heterodimeric integrin molecule αEβ7, which has no distinct name. ... "The epithelial cell-specific integrin, CD103 (alpha E integrin), defines a novel subset of alloreactive CD8+ CTL". J. Immunol. ... "Entrez Gene: integrin". Lehmann J, Huehn J, de la Rosa M, Maszyna F, Kretschmer U, Krenn V, Brunner M, Scheffold A, Hamann A ( ...
It is known to complex with integrins. This gene is associated with X-linked mental retardation and neuropsychiatric diseases ... Berditchevski F (2002). "Complexes of tetraspanins with integrins: more than meets the eye". J. Cell Sci. 114 (Pt 23): 4143-51 ... 1999). "Selective tetraspan-integrin complexes (CD81/alpha4beta1, CD151/alpha3beta1, CD151/alpha6beta1) under conditions ... 2001). "Involvement of alpha3 integrin/tetraspanin complexes in the angiogenic response induced by angiotensin II". FASEB J. 15 ...
2015). "Tumour exosome integrins determine organotropic metastasis". Nature 527(7578):329-35. Epub 2015 Oct 28. Duarte, Delfim ...
... integrin-RGD bonds are considerably weaker than integrin-fibronectin bonds. The reduced adhesion strength is likely due to both ... These integrins include αvβ3, α5β1 and αIIbβ3. The RGD domain is both sufficient and indispensable for cell membrane binding. ... For example, the RGD peptide can be used to target cancer cells on which cell membrane integrins are up-regulated compared to ... The family of membrane proteins known as integrins act as receptors for these cell adhesion molecules via the RGD motif. A ...
Integrins and connexins are markedly up-regulated. A key role in this concerted action, which leads to a 2-to-20 fold increase ...
alphav-beta3 integrin-IGF-1-IGF1R complex. • alphav-beta3 integrin-PKCalpha complex. • alphav-beta3 integrin-HMGB1 complex. • ... integrin complex. • platelet alpha granule membrane. • integrin alphav-beta3 complex. • extracellular exosome. • lamellipodium ... Integrin beta 3 is found along with the alpha IIb chain in platelets. Integrins are known to participate in cell adhesion as ... The ITGB3 protein product is the integrin beta chain beta 3. Integrins are integral cell-surface proteins composed of an alpha ...
Integrin expression may also be regulated by miRNAs, which can also modulate integrin signaling and function. Integrins are ... Similarly, α4 also associates with β7 while integrin β7 also associates with αE. Integrin β1 and integrin β4 are regulated by ... there are no integrin α subunits known to be regulated by miRNA. As for the RGD ligand integrins, integrin α5 is the only α ... Integrin β2 associates with αD, αL,αM, and αX, and is exclusively expresse on the surface of leukocytes. To date, integrins β2 ...
... J Cell Sci. 2009 Jan 15;122(Pt 2):215-25. doi: 10.1242/jcs.019117. ... Their ability to penetrate into tissues and to make contacts with other cells depends chiefly on the beta2 integrin known as ... In this Commentary, we highlight, from a cell-biology point of view, the integrin-mediated immune-cell migration and cell-cell ... and this holds true particularly for the functioning of the integrins on these cells. ...
Getting integrins into shape: recent insights into how integrin activity is regulated by conformational changes. J Cell Sci ... Synaptic integrins in developing, adult, and mutant muscle: selective association of al, a7A and a7B integrins with the ... Porter JC, Hogg N. Integrins take partners: cross-talk between integrins and other membrane receptors. Trends Cell Biol 1998; 8 ... Hynes R. Integrins, versatility, modulation, and signaling in cell adhesion. Cell 1992; 69: 11-25.PubMedGoogle Scholar ...
Integrins are found in all animals while integrin-like receptors are found in plant cells. Integrins work alongside other ... The following are 16 of the ~24 integrins found in vertebrates: Beta-1 integrins interact with many alpha integrin chains. Gene ... integrins of the β2 family). This α-I domain is the binding site for ligands of such integrins. Those integrins that dont ... Which ligand in the ECM the integrin can bind to is defined by which α and β subunits the integrin is made of. Among the ...
Thus, CD98hc is not required for integrin expression, integrin affinity, or integrin-mediated adhesion. ... 5 D and E ). Thus, the portion of CD98hc that binds β1 integrins and enhances integrin signaling promotes tumorigenesis and ... Thus, CD98hc is an integrin-associated protein that mediates integrin-dependent signals, which promote tumorigenesis. ... that binds to β1 integrins stimulates teratocarcinoma growth and mediates integrin signaling indicates that the β1 integrin- ...
Integrin signaling is bidirectional. Inside-out signals regulate integrin affinity for adhesive ligands, and ligand-dependent ... Integrin regulation.. Ginsberg MH1, Partridge A, Shattil SJ.. Author information. 1. Department of Medicine, University of ... Integrin extracellular domains are yielding to high-resolution structural analyses, and intracellular proteins involved in ... integrin signaling are being identified. However, a key unresolved question is how integrins propagate signals across the ...
... leukocyte integrins im Englisch-Deutsch-Wörterbuch, mit echten Sprachaufnahmen, Illustrationen, Beugungsformen, ... ... leukocyte integrin. • leukocyte integrins. leukocyte migration. leukocyte phosphatase. leukocyte picture. leukocyte typing. ... Im Forum nach leukocyte integrins suchen. » Im Forum nach leukocyte integrins fragen. Recent Searches ... leukocyte integrins in anderen Sprachen:. Deutsch - Englisch. Eintragen in .... English - Bulgarian. English - Bosnian. English ...
The β3 integrin was found to coprecipitate with the αV integrin antibody, from which the authors conclude that αVβ3 integrin ( ... Previous investigations have suggested that integrins, in particular the β3 integrin, is involved in the eutrophic remodeling ... β3 integrin, whereas Martinez-Lemus et al12 found involvement of both αVβ3 and αVβ1 integrins in myogenic responsiveness to ... 14 enzymes that are closely associated with integrins.15 There are thus several ways by which the blocking of integrins in the ...
Integrin alphaXbeta2 (p150,95, CR4) is a complement receptor composed of CD11c and CD18. Integrin+alphaXbeta2 at the US ...
The interactions between integrins and the extracellular matrix have been identified as important regulators of vascular cell ... The interactions between integrins and the extracellular matrix have been identified as important regulators of vascular cell ... Strömblad, S., & Cheresh, D. A. (1996). Integrins, angiogenesis and vascular cell survival. Chemistry and Biology. Elsevier Ltd ...
In the inactive state, the two parts of the integrin are bound to one another (and this is what keeps the integrin in the ... However, it has recently emerged that kindlin also plays a role in integrin activation. The exact role of kindin in integrin ... On the left is the inactive integrin (bent over, not bound to the extracellular matrix), and on the right is the active ... In this animation, the red and blue spheres are contacts between the two integrin parts, and you can see that these contacts ...
Advances in the past two years have shed light on the structural basis for integrin regulation and signaling, especially on how ... Integrins are cell adhesion molecules that play critical roles in development, wound healing, hemostasis, immunity and cancer. ... Integrin structures and conformational signaling Curr Opin Cell Biol. 2006 Oct;18(5):579-86. doi: 10.1016/j.ceb.2006.08.005. ... Integrins are cell adhesion molecules that play critical roles in development, wound healing, hemostasis, immunity and cancer. ...
integrin heterodimer is a component of hemidesmosomes," Proceedings of the National Academy of Sciences of the United States of ... integrin subunit," Journal of Cell Biology, vol. 136, no. 6, pp. 1333-1347, 1997. View at Publisher · View at Google Scholar ... integrins and assembly of hemidesmosomes in an in vitro model of wound healing," Journal of Cell Biology, vol. 115, no. 6, pp. ... integrins as therapeutic targets in autoimmune disease," New England Journal of Medicine, vol. 348, no. 1, pp. 68-72, 2003. ...
... αV-integrins via RGD motif that resides on virus capsid. In contrast, echovirus 1 (E-1) has no RGD and uses integrin α2β1 as ... β1 integrin and enters via macropinocytosis. In this paper, we review what is known about receptors and endocytosis of integrin ... Endocytosis of Integrin-Binding Human Picornaviruses. Pirjo Merilahti,1,2 Satu Koskinen,1 Outi Heikkilä,1,2 Eveliina Karelehto, ... Three enterovirus types and one parechovirus have experimentally been shown to bind and use integrin receptors in cellular ...
An integrin protein in a cell membrane moves like a sunflower seeking the sun, connecting every animal cell to its environment ... An integrin protein in a cell membrane moves like a sunflower seeking the sun, connecting every animal cell to its environment ...
AG Extracellular Matrix and Integrins in Cardiovascular Disease. Integrin signaling and heart failure Integrins are ... Integrin activation and atherosclerosis Although integrins are highly expressed on the cell surface, they do not bind their ... ß2 integrin activation on T cells is also a critical step during the process of extravasation and recruitment of T cells within ... Beta2-integrin activation on T cell subsets is an independent prognostic factor in unstable angina pectoris. Basic Res Cardiol ...
IPR015812. Integrin_bsu. IPR014836. Integrin_bsu_cyt_dom. IPR002369. Integrin_bsu_VWA. IPR032695. Integrin_dom_sf. IPR036465. ... IPR015812. Integrin_bsu. IPR014836. Integrin_bsu_cyt_dom. IPR002369. Integrin_bsu_VWA. IPR032695. Integrin_dom_sf. IPR036465. ... Integrin betaUniRule annotation. ,p>Information which has been generated by the UniProtKB automatic annotation system, without ... Integrin betaSequence analysis. ,p>Information which has been generated by the UniProtKB automatic annotation system, without ...
Read about integrins and their associated proteins and importance in human disease. Find antibodies, proteins, modulators and ... Integrins, associated proteins and disease. Integrins are a family of cell surface transmembrane receptors, each consisting of ... While most integrins connect to the actin cytoskeleton and reside in adhesion structures, called focal adhesions, integrin α6β4 ... His main objective is to decipher the function of integrins in differentiation and migration, and how integrins and associated ...
Integrins are cell‐surface adhesion molecules formed from eight different β chains and 18 different α chains that assemble as ... Integrin subunit pairings. There are 18 α‐ and 8 β‐integrin subunits from which 24 different functional integrins are currently ... The integrin superfamily first emerged in the metazoa and has expanded with evolution creating 24 different integrins in humans ... de Melker AA and Sonnenberg A (1999) Integrins: alternative splicing as a mechanism to regulate ligand binding and integrin ...
Mice Lacking αv Integrins Develop Colitis.. Determining the precise contributions of αv integrins to immune functions in vivo ... Ulcerative colitis and autoimmunity induced by loss of myeloid αv integrins. Adam Lacy-Hulbert, Aileen M. Smith, Hamid Tissire ... Ulcerative colitis and autoimmunity induced by loss of myeloid αv integrins. Adam Lacy-Hulbert, Aileen M. Smith, Hamid Tissire ... Deletion of αv integrins in endothelial and hematopoietic cells in αv-tie2 mice. (a and b) Small intestine from tie2-CRE ...
Embryos mutant for β-integrin (A,mys), αPS1 integrin (B,mew), αPS2 integrin (C,if), and αPS3 integrin (D,scab) have a range of ... Genes for integrin ligands interact with slit. The midline axon phenotype of integrin mutants is part of a more complex ... How do integrins contribute to axon guidance?. Integrins are concentrated in the growth cones ofDrosophila axons (Takagi et al ... Integrin phenotypes in the CNS do not demonstrate a direct role for integrins in growth cone guidance. In contrast, ...
Recently ADAP was shown genetically to positively regulate T cell activation, TCR-induced integrin clustering, and T cell ... The mechanism by which ADAP couples TCR stimulation to integrin clustering remains unclear; however, studies of ADAP, the ... exchange factor Vav1, and WASP suggest that TCR and integrin clustering may be controlled by distinct signaling pathways. ...
They found that OSCC-3 attached to RGD-alginate in an α5β1 integrin-dependent manner and proliferated when attached to RGD- ... Cancer cell angiogenic capability is regulated by 3D culture and integrin engagement. Proc. Natl. Acad. Sci. U.S.A. 106, 399- ... which constitute the primary integrin recognition site in many extracellular matrix (ECM) proteins. ...
IPR013649 Integrin_alpha-2. IPR018184 Integrin_alpha_C_CS. IPR028994 Integrin_alpha_N. IPR032695 Integrin_dom_sf. IPR002035 VWF ... IPR013649 Integrin_alpha-2. IPR018184 Integrin_alpha_C_CS. IPR028994 Integrin_alpha_N. IPR032695 Integrin_dom_sf. IPR002035 VWF ... IntegrinUniRule annotation. ,p>Information which has been generated by the UniProtKB automatic annotation system, without ... Belongs to the integrin alpha chain family.UniRule annotation. Automatic assertion according to rulesi ...
reacts with the integrin heterodimer of 165 kDa which comprises the CD11b receptor ...
integrin definition: nounAny of a group of transmembrane proteins that bind to certain molecules in the extracellular matrix or ... integrin. in·te·grin. noun. Any of a group of transmembrane proteins that bind to certain molecules in the extracellular matrix ... plural integrins). *(biochemistry) Any of many heterodimeric transmembrane proteins that function as receptors in communication ... "integrin." YourDictionary, n.d. Web. 15 January 2019. ,https://www.yourdictionary.com/integrin,. ...
... the crystal structure of one of the many integrins, proteins that play key roles in such important activities as cell division ...
The integrins are a family of transmembrane receptors important for the cell-cell and cell-matrix interactions that relate to ... The acquisition of integrins such as αvβ3 has been correlated to the process of tumor progression in multiple tissues, ... The integrins are a family of transmembrane receptors important for the cell-cell and cell-matrix interactions that relate to ... The integrins are a family of transmembrane receptors important for the cell-cell and cell-matrix interactions that relate to ...
... labs at UCSF has resulted in an unprecedented look at a member of a vital and ubiquitous class of proteins called integrins. ... Integrins are embedded on the surfaces of all animal cells, connecting each cell to its surroundings and allowing it to ... To explore an integrins structure, the team used cryo-electron microscopy, a technique that has recently benefited from major ... In order to encounter its targets, the new work suggests for the first time that an active integrin bends and sways at a ...
  • Collagen ligand integrins include integrins α1 and α2 subunits, known to be regulated by specific miRNAs. (nih.gov)
  • Among the laminin ligand integrins, there are no integrin α subunits known to be regulated by miRNA. (nih.gov)
  • Finally, among the α subunits that comprise the leukocyte-specific receptor ligand integrins, integrins αD, αL, αM, and αX have shown regulation by different miRNAs. (nih.gov)
  • As for the integrin β subunits, regulation by miRNAs has been reported for all but β5 and β6 to date. (nih.gov)
  • Integrin β1 and its α subunits form multiple heterodimers. (nih.gov)
  • Integrins are obligate heterodimers composed of α and β subunits. (wikipedia.org)
  • Several genes code for multiple isoforms of these subunits, which gives rise to an array of unique integrins with varied activity. (wikipedia.org)
  • In mammals integrins are assembled from eighteen α and eight β subunits, in Drosophila five α and two β subunits, and in Caenorhabditis nematodes two α subunits and one β subunit. (wikipedia.org)
  • Through different combinations of the α and β subunits, 24 unique mammalian integrins are generated, excluding splice- and glycosylation variants. (wikipedia.org)
  • Integrin subunits span the cell membrane and have short cytoplasmic domains of 40-70 amino acids. (wikipedia.org)
  • The molecular mass of the integrin subunits can vary from 90 kDa to 160 kDa. (wikipedia.org)
  • The cations in the β subunits are more interesting: they are directly involved in coordinating at least some of the ligands that integrins bind. (wikipedia.org)
  • In mammals, 24 different integrins are generated through different combinations of 18 α and 8 β subunits. (abcam.com)
  • In humans, 24 different integrins are formed from 18 α and 8 β subunits. (els.net)
  • Integrin β subunits contain four repeats of an eight cysteine motif resembling EGF‐like motifs. (els.net)
  • There are 18 α‐ and 8 β‐integrin subunits from which 24 different functional integrins are currently known to be formed. (els.net)
  • Integrins are dimeric cell-surface receptors composed of α and β subunits ( 1 ). (pnas.org)
  • Here we show that knockdown of integrin subunits slows down the progression of hepatocellular carcinoma (HCC). (mit.edu)
  • Using nanoparticulate delivery of short interfering RNAs targeting β1 and αv integrin subunits, we downregulate all integrin receptors in hepatocytes. (mit.edu)
  • Here we conditionally deleted all the α subunits that form predominant laminin-binding β1 integrins in Schwann cells and show that only α6β1 and α7β1 integrins are required and that α7β1 compensates for the absence of α6β1 during development. (jneurosci.org)
  • Integrins are obligate heterodimers containing two distinct chains, called the α (alpha) and β (beta) subunits. (wikidoc.org)
  • Through different combinations of the α and β subunits, some 24 unique integrins are generated, although the number varies according to different studies. (wikidoc.org)
  • Composed of alpha and beta subunits, integrin receptors form structural and functional linkages between the ECM and intracellular cytoskeletal linker proteins [2] . (thermofisher.com)
  • When compared with other I domain-containing collagen-binding integrin subunits, α11 amino acid sequence shows the highest overall identity with integrin alpha 10 (42% identity) followed by integrin alpha 1 (37% identity), and integrin alpha 2 (35% identity). (atlasgeneticsoncology.org)
  • We show that two known viable mutations, one which has its primary effect on the fly's escape response, the other on wing morphogenesis, are mutations in the beta and PS2alpha subunits, respectively, of the PS integrins. (biologists.org)
  • Our data are for the most part strikingly different from previous results obtained in vitro and significantly revise proposed models for the role of serine and tyrosine phosphorylation and the function of a salt bridge between the integrin beta subunits and the integrin alpha tails. (uni-muenchen.de)
  • In this context, the colon tumorigenesis observed in mice carrying a total gut epithelial-specific deletion of the α6 integrin subunits may need to be interpreted with caution. (bmj.com)
  • Three major subfamilies of integrins have been defined, each containing a common beta subunit that can be associated with multiple alpha subunits. (humpath.com)
  • Three integrin subfamilies were originally defined on the basis of which of three β subunits were used to form the heterodimers. (humpath.com)
  • Most metazoan cells dynamically interact with ECM components via integrins, which are heterodimeric transmembrane proteins composed of α and β subunits ( 2 ). (aacrjournals.org)
  • ILK can anchor to integrins by interacting with the cytoplasmic domain of certain β integrin subunits. (rupress.org)
  • The CD18 (β 2 ) integrin subfamily is comprised of four members sharing a common β subunit associated with the following four distinct but structurally homologous α subunits: LFA-1 (α L β 2 , CD11a/CD18), Mac-1 (α M β 2 , CD11b/CD18), p150,95 (α X β 2 , CD11c/CD18), and α D β 2 (CD11d/CD18) ( 6 , 7 ). (jimmunol.org)
  • Thus far, many studies on the endo/exocytic cycle of integrins have focused on the cytoplasmic domains of the β subunits. (biologists.org)
  • Integrins are a family of transmembrane proteins that both regulate cell matrix interactions and serve as receptors that mediate intracellular signaling and a variety of cellular processes, including inflammatory responses, immunoresponses, and tumorigenesis. (nih.gov)
  • In addition, each integrin can be classified into 1 of 4 groups based on its extracellular binding ligand: collagen, laminin, RGD (Arg-Gly-Asp) or leukocyte-specific receptors. (nih.gov)
  • Based on the different extracellular ligands, integrin β1 heterodimers can be classified into four groups: collagen ligand, RGD ligand, laminin ligand and leukocyte-specific expressing receptors. (nih.gov)
  • Integrins are transmembrane receptors that facilitate cell-cell and cell-extracellular matrix (ECM) adhesion. (wikipedia.org)
  • Upon ligand binding, integrins activate signal transduction pathways that mediate cellular signals such as regulation of the cell cycle, organization of the intracellular cytoskeleton, and movement of new receptors to the cell membrane. (wikipedia.org)
  • Integrins are found in all animals while integrin-like receptors are found in plant cells. (wikipedia.org)
  • Integrins receive signals from other receptors that lead to activation of ligand binding (inside-out signaling) and matrix assembly. (nih.gov)
  • Recent findings indicate that adhesion chemistries and, most particularly, activities of integrin class adhesion receptors play roles in each of these processes and thereby are likely to contribute significantly to the cell biology underlying epileptogenesis. (springer.com)
  • Three enterovirus types and one parechovirus have experimentally been shown to bind and use integrin receptors in cellular infection. (hindawi.com)
  • In this paper, we review what is known about receptors and endocytosis of integrin-binding human picornaviruses. (hindawi.com)
  • Integrins are heterodimeric surface receptors consisting of one α-chain and one ß-chain. (uni-heidelberg.de)
  • In a DFG funded project we are currently dissecting the role of integrin receptors in cardiac hypertrophy in vitro and in vivo by applying the conditional Tx-driven Cre-lox technology. (uni-heidelberg.de)
  • Integrins are a family of cell surface transmembrane receptors, each consisting of an α and a β subunit that bind to extracellular matrix (ECM) proteins and cellular counter receptors and associate with the cytoskeleton. (abcam.com)
  • Integrins are cell‐surface adhesion molecules formed from eight different β chains and 18 different α chains that assemble as heterodimeric transmembrane receptors to mediate cell-cell and cell-matrix interactions. (els.net)
  • Integrins are heterodimeric transmembrane receptors that mediate cell adhesion to the microenvironment. (els.net)
  • Integrins recognise a wide range of ligands, including extracellular matrix proteins, counter receptors on neighbouring cells, plasma proteins, and microbes and viruses. (els.net)
  • Integrin receptors provide a primary means of cell interaction with the extracellular matrix (ECM). (jneurosci.org)
  • Association of integrin receptors with ECM ligands is modeled to provide the physical linkage and adhesive signaling that stabilizes the actin cytoskeleton and enables growth cone extension. (jneurosci.org)
  • The integrins are a family of transmembrane receptors important for the cell-cell and cell-matrix interactions that relate to processes of cell adhesion, migration, invasion, angiogenesis, and survival. (cancernetwork.com)
  • This article will focus on recent advances in our understanding of intracellular signal transduction pathways regulated by the integrin family of adhesion receptors. (sciencemag.org)
  • In a report online in the Proceedings of the National Academy of Sciences, Estes and her colleagues describe two receptors for the enterotoxin, both of them integrins. (biologynews.net)
  • Integrins are transmembrane receptors that mediate the attachment between a cell and its surroundings, such as other cells or the extracellular matrix (ECM). (wikidoc.org)
  • The authors ascribe the Dnm2 knockdown-induced defect in vessel formation at least partially to disrupted integrin turnover: focal adhesion size is increased and inactive integrin receptors appear to accumulate on the cell surface. (biologists.org)
  • Integrins are cell surface receptors that interact with the extracellular matrix. (thermofisher.com)
  • The integrin family of proteins is the major cell surface receptors involved in mediating cellular response to ECM binding. (thermofisher.com)
  • In addition to actin polymerization/depolymerization, ligand binding to integrin receptors results in the Talin-mediated oligomerization of FAK (Focal Adhesion Kinase). (thermofisher.com)
  • Integrins are a family of conserved cell surface receptors found throughout the animal kingdom. (diva-portal.org)
  • Integrins are cell-surface receptors that mediate and coordinate cellular responses to the extracellular matrix . (humpath.com)
  • The integrin family (ITGs) includes cell surface receptors for extracellular matrix components as well as receptors involved in various aspects of leukocyte adhesion. (humpath.com)
  • Integrins are a large family of trans membrane receptors for extracellular matrix (ECM) molecules. (humpath.com)
  • Given the role of endothelial α v β 3 integrin receptors in angiogenesis ( 18 ) and the antiangiogenic potential of α v β 3 integrin antagonists ( 19 - 22 ), this observation raises the hypothesis that inhibition of α v β 3 integrin function may concur to the angiosuppressive activity of resveratrol. (aacrjournals.org)
  • Integrins are the major family of cell adhesion receptors in humans and essential for a wide range of normal physiology, including formation and maintenance of tissue structure integrity, cell migration, proliferation, and differentiation. (onmedica.com)
  • Integrins are the major cell surface adhesion receptors for ligands in the extracellular matrix. (biologists.org)
  • which suggests that integrin traffic provides the cell with a constant supply of `refreshed' receptors that can bind ligand. (biologists.org)
  • Integrins are a large family of transmembrane cell adhesion receptors that are found on the surface of eukaryotic cells. (uwaterloo.ca)
  • Integrins act predominantly as cell surface receptors for extracellular matrix (ECM) proteins, but also have bidirectional signaling properties that allow them to play fundamental roles in development and cancer metastasis. (uwaterloo.ca)
  • Integrins are integral cell-surface proteins composed of an alpha chain and a beta chain. (wikipedia.org)
  • Integrins are heterodimer adhesion proteins comprised of an α and a β subunit. (nih.gov)
  • Integrins work alongside other proteins such as cadherins, the immunoglobulin superfamily cell adhesion molecules, selectins and syndecans, to mediate cell-cell and cell-matrix interaction. (wikipedia.org)
  • An acidic amino acid features in the integrin-interaction site of many ECM proteins, for example as part of the amino acid sequence Arginine-Glycine-Aspartic acid ("RGD" in the one-letter amino acid code). (wikipedia.org)
  • Many of these tasks involve a family of proteins called integrins, which act as the "hands" of the cell. (scienceblogs.com)
  • His main objective is to decipher the function of integrins in differentiation and migration, and how integrins and associated proteins regulate the assembly of multiprotein complexes in normal and pathological conditions. (abcam.com)
  • In a process called "outside-in" signaling, the engagement of integrins results in the recruitment of cytoskeletal proteins to sites of cell-ECM contacts, thereby promoting integrin clustering and driving the formation of focal adhesions. (abcam.com)
  • Through their ability to bind actin filaments, these cytoskeletal proteins may influence the clustering of integrins and/or the stabilization of clustered integrins and thus regulate integrin activity. (abcam.com)
  • Integrins not only associate with cytoskeletal proteins, but they can also engage in lateral associations with members of the tetraspanin-4 family. (abcam.com)
  • investigated angiogenic signaling in oral squamous cell carcinoma cells (OSCC-3) cultured in a two- or three-dimensional matrix composed of alginate coupled to Arg-Gly-Asp (RGD) peptides, which constitute the primary integrin recognition site in many extracellular matrix (ECM) proteins. (sciencemag.org)
  • In work published online this week by Science ( www.sciencexpress.org ), researchers have determined, for the first time, the crystal structure of one of the many integrins, proteins that play key roles in such important activities as cell division and migration. (sciencemag.org)
  • Integrins are associated with fibrosis, scarring and stiffening of tissues that is associated with nearly half of all deaths in developed countries, and yet researchers had no high-resolution structural model of the proteins in their active state. (ucsf.edu)
  • This method works best on stationary, rigid, and symmetrical proteins: the opposite of integrins, which are quite flexible in their active form, said Stephen Nishimura , MD, one of the paper's senior authors and a professor of pathology at UCSF. (ucsf.edu)
  • Proteomic analysis reveals that Rab11b controls the cell surface proteome, recycling proteins required for successful interaction with the microenvironment, including integrin β1. (nature.com)
  • Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. (wikipedia.org)
  • To find antibodies, assays, and proteins associated with the integrin pathway, click on the icon for your desired gene of interest or click here to see the list of genes below the graphic. (thermofisher.com)
  • Cell surface proteins such as integrins have been suggested to transmit physical stimuli from the tendon ECM to regulate intracellular signaling pathways and gene expression 2 . (nature.com)
  • Heterodimers of α and β integrins transduce different mechanotransduction pathways, depending on the engaged isoforms and their interactions with the ECM and cytoplasmic proteins 3 . (nature.com)
  • Integrins are transmembrane heterodimer proteins, consisting of an alpha (α) and beta (β) chain. (biolegend.com)
  • Integrin αvβ3 mediates cell adhesion to several extracellular matrix proteins in the bone microenvironment which is necessary for tumor cell colonization as well as the transmission of mechanical signals for tumor progression. (mdpi.com)
  • β 2 integrins have been reported to form complexes with other plasma membrane proteins such as CD63 (( 10 )), the immunoglobulin receptor FcγRIIIB (CD16b) (( 11 )) and the urokinase receptor (uPAR, CD87) (( 12 ), ( 13 )) suggesting possible functional interaction. (rupress.org)
  • Integrins cluster in cell-matrix adhesions where they connect the ECM to the F-actin network via scaffolding proteins such as talin, vinculin, and paxillin. (rupress.org)
  • Integrins are cell adhesion proteins that are normally inactive on the surface of cells. (fold.it)
  • The integrin superfamily consists of about 30 structurally homologous proteins that promote cell-cell or cell-matrix interactions . (humpath.com)
  • All integrins are heterodimeric cell surface proteins composed of two noncovalently linked polypeptide chains, α (ITGAs) and β (ITGBs). (humpath.com)
  • The other integrins are expressed on platelets and other cell types, and bind to extracellular matrix proteins as well as proteins involved in coagulation . (humpath.com)
  • The integrin cytoplasmic tails interact with the cytoskeleton, signaling molecules and other cellular proteins, resulting in regulation of many biological functions . (humpath.com)
  • Gens SJ, Reuzeau C, Dolittle KW, McNally JG, Pickard BG (1996) Covisualization by computational optical-sectioning microscopy of integrin and associated proteins at the cell membrane of living onion protoplasts. (springer.com)
  • Marcantonio E, Hynes RO (1988) Antibodies to the conserved cytoplasmic domain of the integrin β 1 subunit react with proteins in vertebrates, invertebrates, and fungi. (springer.com)
  • Such regulatory mechanisms occur after extracellular stimuli (e.g., growth factors or cytokines), alter intracellular effector proteins that in turn bind to integrin cytoplasmic regions, and induce conformational changes in the integrin extracellular domains ( 2 ). (aacrjournals.org)
  • These various integrins recognize argine-glycine-aspartic acid (RGD) peptide sequences found in many ECM proteins. (aacrjournals.org)
  • Recent biochemical, cell biological, and genetic studies have revealed important roles of cytoplasmic integrin-linked kinase (ILK) and its interactive proteins in these processes. (rupress.org)
  • Upon adhesion to ECM, integrins and a selective group of cytoskeletal and signaling proteins are recruited to cell matrix contact sites where they link the actin cytoskeleton to the ECM and mediate signal transduction between the intracellular and extracellular compartments. (rupress.org)
  • A) This figure depicts the interaction of ILK with various proteins many of which, including ILK, are found at sites of integrin connections to the actin cytoskeleton. (rupress.org)
  • CH-ILKBP, paxillin, and probably other ILK-binding proteins can bind directly or indirectly to actin filaments, thus coupling integrins to the actin cytoskeleton via ILK. (rupress.org)
  • The CD51/CD61 integrin binds to matrix proteins including vitronectin, fibronectin, vWF and fibrinogen, and has been shown to have a strong role in modulating the migration and survival of angiogenic endothelial cells. (fishersci.com)
  • Ligation of integrin by matrix proteins such as fibronectin is important for cell migration and leads to assembly of focal adhesion proteins at the site of attachment. (biologists.org)
  • Integrins: emerging paradigms of signal transduction. (nih.gov)
  • In signal transduction , integrins pass information about the chemical composition and mechanical status of the ECM into the cell. (wikidoc.org)
  • This study provides the first direct evidence for the possibility of integrin-mediated signal transduction in the perception of gravity by higher plants. (springer.com)
  • Following activation and engagement with ECM ligands, integrins regulate cytoskeletal dynamics as well as intracellular signal transduction cascades that lead to a wide variety of cellular responses, including proliferation, differentiation, and survival. (aacrjournals.org)
  • Giancotti: Signal transduction by the alpha 6 beta 4 integrin: charting the path between laminin binding and nuclear events. (antikoerper-online.de)
  • Integrin beta-3 (β3) or CD61 is a protein that in humans is encoded by the ITGB3 gene . (wikipedia.org)
  • The ITGB3 protein product is the integrin beta chain beta 3. (wikipedia.org)
  • Thus, CD98hc is an integrin-associated protein that mediates integrin-dependent signals, which promote tumorigenesis. (pnas.org)
  • The cell internally controls whether an integrin is adhesive by signals from within the cell, using another protein called talin. (scienceblogs.com)
  • Ben's paper explores a different aspect of integrin activation: the role of another protein, kindlin. (scienceblogs.com)
  • An integrin protein in a cell membrane moves like a sunflower seeking the sun, connecting every animal cell to its environment. (youtube.com)
  • With this technique, we can demonstrate that ß2 integrin activation on T cells in patients with acute coronary syndrome (ACS), precedes future cardiovascular events and provides additional prognostic information over established biomarkers such as cardiac troponin (cTnT) or high-sensitive C-reactive protein (hsCRP) (6). (uni-heidelberg.de)
  • The adhesive potential of integrins can be regulated and their cytoplasmic tails connect through a dynamic protein interaction network to the cytoskeleton. (els.net)
  • Integrins couple the extracellular microenvironment to the cytoskeleton through a protein interaction network associated with their cytoplasmic tails. (els.net)
  • Melody Campbell , PhD, worked to visualize one type of integrin protein down to near-atomic precision. (ucsf.edu)
  • Integrin alpha-IIb is a protein that in humans is encoded by the ITGA2B gene . (wikipedia.org)
  • Integrins are likely to play an important role in transmitting mechanical stimuli from the extracellular matrix to tendon cells, thereby triggering cell signaling pathways which lead to adaptive regulation of mRNA translation and protein synthesis. (nature.com)
  • Integrins span the cell membrane so that a part of the protein is outside the cell and another part is in the interior. (ubc.ca)
  • The cytoplasmic tail of integrins serve as a binding site for α-actinin and Talin which then recruit vinculin, a protein involved in anchoring F-actin to the membrane [3] . (thermofisher.com)
  • Even SBA had no physical connection with integrins, an association was detected between SBA and α-actinin-2 ACTN2 (integrin-binding protein). (mdpi.com)
  • Further trials are needed to prove whether ACTN2 is the only protein for connecting SBA with integrin. (mdpi.com)
  • Review on ILK (integrin-linked kinase), with data on DNA, on the protein encoded, and where the gene is implicated. (atlasgeneticsoncology.org)
  • The items Mantoux, download i domain integrins paper organism, protein century reaction, and PPDs retain as silenced subsequently. (lightseed.com)
  • Integrin is a very large protein, and this puzzle includes only the binding portion of integrin. (fold.it)
  • Here we report on the presence of a β 1 integrin-like protein localized inside amyloplasts of tobacco NT-1 suspension culture, callus cells, and whole-root caps. (springer.com)
  • Biochemical data reveals a 110-kDa protein immunoprecipitated from membrane fractions of NT-1 suspension culture indicating size homology to known β 1 integrin in animals. (springer.com)
  • Katembe WJ, Swatzell L, Makaroff CA, Kiss JZ (1996) Immunolocalization of integrin-like protein in Arabidopsis and Chara . (springer.com)
  • In contrast, elevated αvβ6 integrin protein expression is associated with advanced stages of human squamous cell carcinomas ( 7 ). (aacrjournals.org)
  • As previously, the first chain of the protein is "integrin alpha-V", but a trimmed-down version with only 116 segments instead of 193. (fold.it)
  • Thus, we identify CD98hc as a membrane protein that enables matrix assembly and establish that it functions by interacting with integrins to support RhoA-driven contractility. (sigmaaldrich.com)
  • In keeping with these observations, trans -resveratrol, but not cis -resveratrol, inhibits α v β 3 integrin-dependent endothelial cell adhesion and the recruitment of enhanced green fluorescent protein-tagged β 3 integrin in focal adhesion contacts. (aacrjournals.org)
  • A gene on chromosome 1q21 that encodes an integrin alpha chain protein which, like all integrins, forms a heterodimer with a beta chain, thereby participating in cell adhesion and cell surface-mediated signalling. (thefreedictionary.com)
  • Zusätzlich bieten wir Ihnen Integrin beta 4 Kits (25) und Integrin beta 4 Proteine (10) und viele weitere Produktgruppen zu diesem Protein an. (antikoerper-online.de)
  • Briefly, 14 C-terminal amino acids of the cytoplasmic domain of β3 integrin, including a crucial unphosphorylated tyrosine (Y759), are involved in binding to protein kinase D1 (PKD1, Fig. 1 ). (biologists.org)
  • One such molecule, GIPC (GAIP-interacting protein, C-terminus), is thought to have a role in regulating α5β1 integrin surface expression, as well as integrin-mediated inside-out and outside-in signaling pathways by mediating the integrin's ability to interact with the ECM protein, fibronectin (FN). (uwaterloo.ca)
  • Ligands for integrins include fibronectin, vitronectin, collagen and laminin. (wikipedia.org)
  • This integrin is known to interact with fibronectin, and indeed mRNA expression of the fibronectin splice form EIIIA+ was doubled in the Ren2 rats. (ahajournals.org)
  • It is quite likely that the reshaping of the SMC depends on integrins cyclically binding to and releasing the extracellular matrix, and the α V β 3 -fibronectin bond is one of the candidates in this process. (ahajournals.org)
  • By establishing a Tamoxifen (Tx) inducible knockout mouse, we have demonstrated that the extracellular matrix glycoprotein fibronectin (Fn), activates critical survival signals via ß1 integrin following myocardial infarction (1). (uni-heidelberg.de)
  • The 2019 Gordon Research Conference on "Fibronectin, Integrins and Related Molecules" will be held May 5th-10th at the Il Ciocco Hotel and Resort, Lucca (Barga), Italy. (grc.org)
  • This GRC will be held in conjunction with the "Fibronectin, Integrins and Related Molecules (GRS)" Gordon Research Seminar (GRS). (grc.org)
  • Integrin alpha-2/beta-1 is a receptor for laminin, collagen, collagen C-propeptides, fibronectin and E-cadherin. (rcsb.org)
  • These effects are mediated in part by fibronectin binding to integrin α5, which recruits and activates phosphodiesterase 4D5 (PDE4D5) by inducing its dephosphorylation on an inhibitory site, S651. (jci.org)
  • Integrins bind cell surface and ECM components such as fibronectin , vitronectin , collagen , and laminin . (wikidoc.org)
  • During wound healing, angiogenesis, and tumor invasion, cells often change their expression profiles of fibronectin-binding integrins. (rupress.org)
  • Several integrins bind to Arg-Gly-Asp (RGD) sequences in the fibronectin and vitronectin molecules. (humpath.com)
  • CD98hc (SLC3A2) participates in fibronectin matrix assembly by mediating integrin signaling. (sigmaaldrich.com)
  • Integrin-dependent assembly of the fibronectin (Fn) matrix plays a central role in vertebrate development. (sigmaaldrich.com)
  • The CD41/CD61 integrin has been shown to be involved in platelet aggregation, and binds to fibrinogen, von Willebrand Factor (vWF) and fibronectin. (fishersci.com)
  • The β 3 integrin was found to coprecipitate with the α V integrin antibody, from which the authors conclude that α V β 3 integrin (the vitronectin receptor) mediates the remodeling. (ahajournals.org)
  • Furthermore, because the antibody did not block the hypertrophic process, indeed enhanced it, this suggests that the α V integrin could be specifically involved in eutrophic remodeling. (ahajournals.org)
  • Anti-Integrin α2 Antibody detects level of Integrin α2 & has been published & validated for use in ELISA, IF, IH, IP, RIA & WB. (sigmaaldrich.com)
  • Each Integrin alpha 9 Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. (novusbio.com)
  • Choose from our Integrin alpha 9 polyclonal antibodies and browse our Integrin alpha 9 monoclonal antibody catalog. (novusbio.com)
  • I'm looking for an efficient anti-human antibody against 1 integrin working in cytometry for extracellular staining. (protocol-online.org)
  • uPAR ligation with a monoclonal antibody induced adhesion of monocytic cells and neutrophils to vascular endothelium by six- to eightfold, whereas ligation with inactivated uPA significantly reduced cell-to-cell adhesion irrespective of the β 2 integrin-stimulating pathway. (rupress.org)
  • The binding specificity and selective antagonism of vedolizumab, an anti-alpha4beta7 integrin therapeutic antibody in development for inflammatory bowel diseases. (semanticscholar.org)
  • In this work, we instead exploit these integrins as a target for antibody Fc effector functions in the context of cancer immunotherapy. (rupress.org)
  • Description: The VI-PL2 monoclonal antibody reacts with human CD61, also known as integrin beta 3 and GPIIIa. (fishersci.com)
  • This α-I domain is the binding site for ligands of such integrins. (wikipedia.org)
  • Other work has demonstrated that seizures, and in some instances subseizure neuronal activity, modulate the expression of integrins and their matrix ligands and the activities of proteases which regulate them both. (springer.com)
  • Integrin affinity for adhesive ligands is regulated by conformational rearrangement of the intracellular and extracellular domains. (abcam.com)
  • The integrin superfamily first emerged in the metazoa and has expanded with evolution creating 24 different integrins in humans, each recognising a distinct subset of extracellular ligands. (els.net)
  • In this study, we document a dosage-dependent phenotypic interaction between genes for the integrins, their ligands, and the midline growth cone repellent, Slit, but not for the midline attractant, Netrin. (jneurosci.org)
  • Drosophila doubly heterozygous for slit and the genes encoding the integrin ligands Laminin A and Tiggrin reveal similar errors in midline axon guidance. (jneurosci.org)
  • Furthermore, a penetrant dosage-dependent interaction of the genes for integrins and their ligands, with the midline repellent slit , suggests a role for integrins in regulating responsiveness to midline axon guidance signals. (jneurosci.org)
  • Changes in either the α or the β subunit of integrin heterodimers alter their specificity for ligands, as summarized in Table I. (springer.com)
  • PSGL-1 participates in E-selectin-mediated progenitor homing to bone marrow: evidence for cooperation between E-selectin ligands and alpha4 integrin. (semanticscholar.org)
  • Integrins are transmembrane heterodimeric glycoproteins, made up of α chains (ITGAs) and β chains (ITGBs), that are expressed on many cell types and bind to ligands on endothelial cells, other leukocytes, and the extracellular matrix. (humpath.com)
  • Most integrins expressed on the cell surface are present in inactive conformations, and their adhesion to ECM ligands must be precisely regulated via "inside-out" activation mechanisms. (aacrjournals.org)
  • Upon activation, Integrin alpha M/beta 2 binds to several ligands, including ICAM-1, Fibrinogen, and the C3 complement fragment C3bi, to mediate phagocyte adhesion, migration and ingestion of complement-opsonized particles. (rndsystems.com)
  • Identification of a talin-binding site in the integrin beta(3) subunit distinct from the NPLY regulatory motif of post-ligand binding functions. (wikipedia.org)
  • As for the RGD ligand integrins, integrin α5 is the only α subunit found to be regulated by miRNAs (miR-31, miR-17-92 cluster, and miR-148 b). (nih.gov)
  • Those integrins that don't carry this inserted domain also have an A-domain in their ligand binding site, but this A-domain is found on the β subunit. (wikipedia.org)
  • The X-ray crystal structure obtained for the complete extracellular region of one integrin, αvβ3, shows the molecule to be folded into an inverted V-shape that potentially brings the ligand-binding sites close to the cell membrane. (wikipedia.org)
  • Advances in the past two years have shed light on the structural basis for integrin regulation and signaling, especially on how global conformational changes between bent and extended conformations relate to the inter-domain and intra-domain shape shifting that regulates affinity for ligand. (nih.gov)
  • Although integrins are highly expressed on the cell surface, they do not bind their respective ligand unless activated from within the cell (inside-out signaling). (uni-heidelberg.de)
  • de Melker AA and Sonnenberg A (1999) Integrins: alternative splicing as a mechanism to regulate ligand binding and integrin signaling events. (els.net)
  • It's a new ligand for binding to integrins," she said. (biologynews.net)
  • 40 1.79*10^6 3.47*10^6 4.00*10^5 a) which ligand leads to the greates degree of dimerization of the integrin-ligand complex at 20C? (physicsforums.com)
  • I'm not sure how to find the tfraction of total integrin-ligand complexes. (physicsforums.com)
  • The association of the laminin-binding integrins (α3β1, α6β1, α6β4 and α7β1) with the tetraspanin CD151 strengthens cell adhesion through mechanisms that include the clustering of the integrins in the plasma membrane. (abcam.com)
  • The absence of either α7β1 or α6β1 integrin impairs the ability of Schwann cells to spread and to bind laminin 211 or 411, potentially explaining the failure to extend cytoplasmic processes around axons to sort them. (jneurosci.org)
  • An integrin alpha subunit that primarily associates with INTEGRIN BETA1 or INTEGRIN BETA4 to form laminin-binding heterodimers. (harvard.edu)
  • To definitively address the role of CD98hc in integrin function and tumorigenesis, we disrupted the CD98hc gene. (pnas.org)
  • Longitudinal tract axons in Drosophila embryos doubly heterozygous for slit and an integrin gene, encoding αPS1, αPS2, αPS3, or βPS1, take ectopic trajectories across the midline of the CNS. (jneurosci.org)
  • Additionally, SBA reduced the mRNA expression of integrins by down regulating the gene expression level of ACTN2 . (mdpi.com)
  • Tapes UK and molecular download i domain integrins, Corrosion, chemical and new university, tuberculin and future from the Financial Times maintenance gene. (lightseed.com)
  • An analysis of lethal mutations in a PS integrin gene showed that the integrins were required for muscle attachment and for certain cell sheet migrations during embryogenesis. (biologists.org)
  • The mutation in the gene for the PS2alpha integrin subunit, inflated, also leads to a failure in wing surface apposition and consequent wing blistering. (biologists.org)
  • To test whether Cdc42 is essential for directed cell migration in mammalian cells and to investigate the cross-talks between integrin and Cdc42 mediated signalling, fibroblastoid cell lines lacking a functional Cdc42 gene were established and analyzed in wound closure assays. (uni-muenchen.de)
  • For example, genetic ablation of the αv integrin gene in epithelial cells of the murine skin leads to development of squamous cell carcinomas ( 6 ), revealing tumor suppressor-like functions for αv integrins during epithelial cell homeostasis. (aacrjournals.org)
  • integrin heterodimer is a component of hemidesmosomes," Proceedings of the National Academy of Sciences of the United States of America , vol. 87, no. 22, pp. 8970-8974, 1990. (hindawi.com)
  • Each integrin is a heterodimer, consisting of one a and one β subunit in a noncovalent complex. (springer.com)
  • Integrin α11 subunit binds integrin beta 1 subunit to form a heterodimer and function as a receptor for interstitial collagens (with highest affinity for collagen type I). It is involved in cell attachment, cell migration and collagen reorganization on mesenchymal non-muscle cells. (atlasgeneticsoncology.org)
  • Alternative splicing of the cytoplasmic domain of the alpha6 subunit (INTEGRIN ALPHA6) results in the formation of A and B isoforms of the heterodimer, which are expressed in a tissue-specific manner. (curehunter.com)
  • C D11b, also known as Integrin alpha M and Macrophage-1 Antigen (MAC-1) alpha subunit, is an Integrin alpha chain family member that binds with the Integrin beta 2 subunit, also called CD18, to form the non-covalent heterodimer Integrin alpha M/beta 2, also known as MAC-1 and Complement Receptor Type 3 (CR3). (rndsystems.com)
  • These predictions will undoubtedly guide future investigations of mechanisms underlying integrin expression mechanism and may ultimately yield new therapeutic tools. (nih.gov)
  • 1999) The integrin alpha v beta 6 binds and activates latent TGF beta 1: a mechanism for regulating pulmonary inflammation and fibrosis. (els.net)
  • Integrins regulate cellular behaviors through signaling pathways, including Rho GTPases and kinases. (pnas.org)
  • CD98hc overexpression leads to anchorage-independent cell growth and tumorigenesis in 3T3 fibroblasts and activates certain integrin-regulated signaling pathways. (pnas.org)
  • The β1 integrins regulate numerous cellular functions by signaling through a variety of biochemical pathways ( 1 , 2 ). (pnas.org)
  • Endocytosis of CV-A9 has recently been shown to occur via a novel Arf6- and dynamin-dependent pathways, while, contrary to collagen binding, E-1 binds inactive β 1 integrin and enters via macropinocytosis. (hindawi.com)
  • however, studies of ADAP, the exchange factor Vav1, and WASP suggest that TCR and integrin clustering may be controlled by distinct signaling pathways. (sciencemag.org)
  • The importance ofmitochondrial ROS for the integrin-induced signaling pathways was investigated using rotenone, a specific inhibitor of complex I in the respiratory chain. (diva-portal.org)
  • These complex cellular responses involve processes such as cell attachment, spreading, and various signaling pathways, which in turn depend on the composition of the extracellular environment, on its mechanical properties, and involved integrin types. (diva-portal.org)
  • The IPP complex, interacts with the cytoplasmic tail of β integrins, resulting in the engagement and organization of the cytoskeleton as well as activation of signalling pathways. (atlasgeneticsoncology.org)
  • Once activated by a binding partner, integrins can induce changes in cell cycle, growth factor signaling pathways, cytoskeletal organization, and movement. (fold.it)
  • Cellular signalling pathways can regulate cell adhesion by altering the affinity and avidity of integrins for the extracellular matrix. (humpath.com)
  • Integrin alphaXbeta2 (p150,95, CR4) is a complement receptor composed of CD11c and CD18. (wikipedia.org)
  • In contrast, echovirus 1 (E-1) has no RGD and uses integrin α 2 β 1 as cellular receptor. (hindawi.com)
  • During angiogenesis, endothelial cell migration is coordinated by integrin‐mediated contact with the extra‐cellular matrix (ECM), coupled with receptor tyrosine kinase signalling to regulate dynamic cytoskeletal and plasma membrane reorganization. (mendeley.com)
  • Integrin alpha-M/beta-2 is also a receptor for fibrinogen, factor X and ICAM1 . (rcsb.org)
  • however, which of the 11 α integrins that can pair with β1 forms the functional receptor is unknown. (jneurosci.org)
  • This review will discuss the αvβ3 integrin receptor in the context of tumor-induced bone disease. (mdpi.com)
  • SB273005 is a potent integrin inhibitor with K i of 1.2 nM and 0.3 nM for αvβ3 receptor and αvβ5 receptor, respectively. (selleckchem.com)
  • The chemokine receptor CCR7 and alpha4 integrin are important for migration of chronic lymphocytic leukemia cells into lymph nodes. (semanticscholar.org)
  • A given chain may combine with multiple partners resulting in different integrins. (wikipedia.org)
  • While most integrins connect to the actin cytoskeleton and reside in adhesion structures, called focal adhesions, integrin α6β4 connects to the intermediate filament (IF) system and is localized in hemidesmosomes. (abcam.com)
  • The association of integrins with the actin cytoskeleton is indirect and involves a number of adaptor molecules. (abcam.com)
  • Kindlin can also form a complex with migfilin and filamin to promote integrin binding to the actin cytoskeleton. (abcam.com)
  • These phenotypes suggest a role for integrins in modulating adhesive signals, perhaps through regulated changes in adhesive affinity, and second messenger signaling to the cytoskeleton and other adhesion molecules ( Hoang and Chiba, 1998 ). (jneurosci.org)
  • Deletion of the genes encoding ILK or PINCH1 similarly blocks maturation of FAs and FBs by downregulating expression or recruitment of tensin and destabilizing α5β1-integrin-cytoskeleton linkages (Legate et al. (atlasgeneticsoncology.org)
  • It involves regulated cell adhesion, mediated by integrins, and polarized changes of the cytoskeleton, controlled by Rho GTPases such as Cdc42. (uni-muenchen.de)
  • Clusters of integrin/ECM interactions form focal adhesions, concentrating cytoskeletal components and signaling molecules within the cell. (thermofisher.com)
  • ILK, PINCH1 and α-parvin form a ternary complex termed IPP (ILK-PINCH-parvin) that localizes to both focal adhesions (FAs) and fibrillar adhesions (FBs) and is essential for several integrin-dependent functions. (atlasgeneticsoncology.org)
  • Focal adhesion kinase modulates cell adhesion strengthening via integrin activation. (biomedsearch.com)
  • β1 integrin (also known as CD29) is a member of the subfamily of collagen-binding integrins (including α1β1, α2β1, α10β1 and α11β1) which bind to the triple-helical GFOGER sequence on collagen fibrils. (nature.com)
  • In this study, we have investigated the role of β1 integrin and ILK in the activation of the AKT/mTOR pathway, and the role of AKT/mTOR in the subsequent expression and translation of collagen in human tendon cells. (nature.com)
  • Alpha 10 binds to a beta-1 chain, forming a collagen type-II-binding integrin, which is expressed in cartilage. (thefreedictionary.com)
  • Integrins are cell adhesion molecules that mediate cell-extracellular matrix and cell-cell interactions. (ebi.ac.uk)
  • 1995) α4 integrins mediate lymphocyte attachment and rolling under physiologic flow. (els.net)
  • αv integrins also mediate the uptake of apoptotic cells ( 12 - 14 ), a process central to immune regulation and inflammation resolution ( 15 ). (pnas.org)
  • All these integrins bind the RGD motif in the central cell-binding domain of FN ( Pankov and Yamada, 2002 ), but it is still unclear if and how they mediate specific cellular responses to FN. (rupress.org)
  • Most of the β1 integrins are widely expressed on leukocytes and other cells and mediate attachment of cells to extracellular matrices. (humpath.com)
  • Integrins are a family of membrane glycoproteins that mediate cell adhesion to extracellular matrix components and to other cells ( 3 , 4 ) and are composed of two noncovalently associated type I transmembrane glycoproteins ( 5 ). (jimmunol.org)
  • All of the major structural changes happen outside of the cell (where the integrin binds to the matrix), but these changes are controlled from inside of the cell, by the binding of talin to the integrin. (scienceblogs.com)
  • CD61 binds non-covalently with the alpha integrins CD41 and CD51, to form the alpha IIb beta3 (CD41/CD61) and alpha v beta3 (CD51/CD61) complexes. (fishersci.com)
  • Although the molecular bases for its antiangiogenic activity are not fully elucidated ( 16 ), recent observations have shown that trans -resveratrol binds purified β 3 integrin chain in vitro ( 17 ). (aacrjournals.org)
  • The integrins generally consist of alpha-beta heterodimeric transmembrane glycoproteins in which the alpha subunit (ITGAs) is noncovalently associated with the beta subunit (ITGBs). (humpath.com)
  • The acquisition of integrins such as α v β 3 has been correlated to the process of tumor progression in multiple tissues, suggesting that approaches effectively targeting the integrins might inhibit or reverse progression. (cancernetwork.com)
  • As such, integrins can play a pervasive role in cancer and tumor metastasis. (biolegend.com)
  • Substantial evidence from preclinical studies shows that increased expression of integrin αvβ3 in tumor cells promotes the metastatic and bone-invasive phenotype. (mdpi.com)
  • These cover oncological applications where integrin targeted microbubbles were used to identify and characterize tumor angiogenesis and to assess tumor response to antiangiogenic drugs as well as to radiotherapy. (biomedsearch.com)
  • Integrin α v β 3 is a marker that is specifically and preferentially overexpressed on multiple tumor types and on angiogenic tumor neovasculature. (dovepress.com)
  • In some types of cancer, integrins are involved in promoting tumor metastasis and cancer blood vessel formation. (fold.it)
  • By combining administration of an engineered mouse serum albumin/IL-2 fusion with an Fc fusion to an integrin-binding peptide (2.5F-Fc), significant survival improvements are achieved in three syngeneic mouse tumor models, including complete responses with protective immunity. (rupress.org)
  • Stromal cells within a tumor microenvironment also play important roles in tumorigenesis and metastases, and many integrins are expressed in tumor-associated stromal components, including fibroblasts, vascular endothelial cells, and inflammatory cells. (aacrjournals.org)
  • furthermore, how altered integrin-ECM interactions then promote tumor cell growth and survival remains elusive. (aacrjournals.org)
  • In this issue of Clinical Cancer Research , Conti and colleagues make an important step toward deciphering how metastatic tumor cells manipulate their repertoire of integrins in response to altered ECM composition of the malignant organ to promote their growth and survival ( 3 ). (aacrjournals.org)
  • Collectively, these data suggest that in certain forms of cancer, αv integrins provide differential functions in tumor initiation versus tumor progression. (aacrjournals.org)
  • Adhesion and signaling functions for αv integrins in regulating metastatic tumor cell growth and survival are not well understood. (aacrjournals.org)
  • address this important topic by analyzing resected liver metastases derived from primary colorectal adenocarcinomas, and show that subpopulations of metastatic tumor cells express elevated levels of αvβ3 and αvβ5 integrins. (aacrjournals.org)
  • Furthermore, they show that tumor cells overexpressing these integrins preferentially reside near regions of tumor-induced fibrosis, or desmoplastic reactions. (aacrjournals.org)
  • Recently, integrins have emerged as a key target in anti-cancer therapy as integrins are involved in promoting tumor metastasis and cancer blood vessel formation. (fold.it)
  • Integrins also play a prominent role in tumor growth and metastasis. (onmedica.com)
  • In this Commentary, we highlight, from a cell-biology point of view, the integrin-mediated immune-cell migration and cell-cell interactions that occur during the course of an immune response. (nih.gov)
  • The interactions between integrins and the extracellular matrix have been identified as important regulators of vascular cell survival, proliferation and invasion during the complex process of blood vessel formation by angiogenesis. (mendeley.com)
  • By exploring the detailed three-dimensional structure of a talin/integrin complex, we showed how key interactions between talin, the integrin, and the inner surface of the cell membrane can elegantly promote the structural changes outside the cell that modulate adhesion strength. (scienceblogs.com)
  • Integrin alpha-M/beta-2 is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles. (rcsb.org)
  • Although there are many different types of CAMs, those know as integrins play an especially crucial role, because integrins are in charge of cell-matrix interactions. (ubc.ca)
  • These data indicate that β 2 integrin-mediated leukocyte-endothelial cell interactions and recruitment to inflamed areas require the presence of uPAR and define a new phenotype for uPAR-deficient mice. (rupress.org)
  • Xenopus provides a useful model system for regulation of integrin function as α5β1-FN interactions are spatially and temporally regulated. (uwaterloo.ca)
  • MicroRNA regulation of integrins. (nih.gov)
  • Kim C , Feng Y and Ginsberg MH (2011) Regulation of integrin activation. (els.net)
  • Here, we demonstrate key roles for αv integrins in regulation of mucosal immunity. (pnas.org)
  • Here, we report that αv integrins are central to the normal regulation of immune responses in the intestine and that deletion of αv in the immune system leads to spontaneous colitis, wasting, and autoimmunity. (pnas.org)
  • Pathologic regulation of integrin-mediated adhesion and signaling is linked to many human diseases, particularly cancer. (aacrjournals.org)
  • The role of integrin endo/exocytic cycle in the regulation of cell adhesion, spreading and motility is becoming increasingly recognized. (biologists.org)
  • Although thrombocytopenia, which is caused by maternal antibodies against β3 integrin and occasionally by maternal antibodies against other platelet antigens, such as glycoprotein GPIbα, has long been assumed to be the cause of bleeding, the mechanism of ICH has not been adequately explored. (jci.org)
  • We offer antibodies, ELISAs, Luminex multiplex assays and growth factors for key targets in the integrin signaling pathway. (thermofisher.com)
  • We offer Integrin alpha 9 Antibodies for use in common research applications: ELISA, Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Paraffin, Sandwich ELISA, Western Blot. (novusbio.com)
  • Our Integrin alpha 9 Antibodies can be used in a variety of model species: Human, Mouse. (novusbio.com)
  • Flow cytometry analysis showing binding of irrelevant anti-c-myc IgG1 ( open histograms, thin line ), mAb 14C5 ( open histograms, thick line ), or integrin antibodies ( gray-filled histograms ) under saturating conditions. (aacrjournals.org)
  • Different anti-integrin antibodies were compared with binding of mAb 14C5 to ( A ) SKBR-3 breast cancer cells and ( B ) Colo16 squamous carcinoma cells. (aacrjournals.org)
  • Antibodies to CD44 and integrin alpha4, but not L-selectin, prevent central nervous system inflammation and experimental encephalomyelitis by blocking secondary leukocyte recruitment. (semanticscholar.org)
  • Two different antibodies to the β 1 integrin, one to the cytoplasmic domain and one to the extracellular domain, localize in the vicinity of the starch grains within amyloplasts of NT-1. (springer.com)
  • For example, differential expression of integrins in primary cutaneous melanoma can be used to distinguish indolent from aggressive, prometastatic melanoma. (onmedica.com)
  • In addition, several kinases regulate integrin traffic. (biologists.org)
  • The identification of their substrates has demonstrated how these kinases regulate integrin traffic by controlling small GTPases or stabilizing cytoskeletal tracks that are crucial for efficient traffic of integrins to the plasma membrane. (biologists.org)
  • CD98 heterodimers, comprised of a heavy chain (CD98hc, SLC3A2) and one of several light chains, interact with integrins through CD98hc. (pnas.org)
  • The cytoplasmic domains of the integrins interact with cytoskeletal components (including vinculin, talin, actin, α-actinin, and tropomyosin). (humpath.com)
  • Schematic illustrations of the structural features of (a) non‐I‐domain and (b) I‐domain integrins. (els.net)
  • following methods updated below the black download i domain integrins with Even mentioned people Specifies the solution for stage while viewing culture in the prose with a current campaign in description book. (lightseed.com)
  • TestLink( ETS) Contains confines of over 20,000 settlers and known download i domain integrins Sets affecting from the mechanical promotions to the engineering. (lightseed.com)
  • Worried to the download i domain integrins of high inclusion, the name of MDR-TB and XDR-TB looks never longer( increasingly to 2 authors or more to strive), gives 8th dynamics that denote less demographic and more additional, and cells demonstrate poorer. (lightseed.com)
  • Here we report that CD98hc contributes to integrin-dependent cell spreading, cell migration, and protection from apoptosis. (pnas.org)
  • Furthermore, CD98hc is required for efficient adhesion-induced activation of Akt and Rac GTPase, major contributors to the integrin-dependent signals involved in cell survival and cell migration. (pnas.org)
  • Here we report that CD98hc-null cells are markedly defective in integrin-dependent cell spreading and cell migration. (pnas.org)
  • The program reflects the interdisciplinary nature of the integrin and extracellular matrix field, spanning different areas of biology from inflammation to mechanobiology, cell migration, stem cells, development, and cancer. (grc.org)
  • Integrins are mainly known for mediating cell and extracellular matrix adhesion and migration. (biolegend.com)
  • Integrin modulation and signaling in leukocyte adhesion and migration. (semanticscholar.org)
  • Here, we show that β1 integrins promote random migration, whereas β3 integrins promote persistent migration in the same epithelial cell background. (rupress.org)
  • Here, we explored the possibility that they promote different aspects of cell migration and that switching integrins allows cells to modulate their motile response to FN. (rupress.org)
  • Aim of this study was to investigate the role of integrins and Cdc42 in cell migration and in particular the cross-talk between these molecules. (uni-muenchen.de)
  • In addition, the structure-function relationship of beta1 integrin in mediating migration associated events was studied. (uni-muenchen.de)
  • Beta1 integrins were previously shown to activate Cdc42 in response to wounding and thus to regulate the directionality of migration. (uni-muenchen.de)
  • Certain RGD-binding integrins are required for cell adhesion, migration, and proliferation and are overexpressed in most tumors, making them attractive therapeutic targets. (rupress.org)
  • Integrin antagonists have already proven successful in halting invasion and migration of tumors. (fold.it)
  • Cell adhesion, migration and the maintenance of cell polarity are all processes that depend on the correct targeting of integrins and the dynamic remodelling of integrin-containing adhesion sites. (biologists.org)
  • Integrins play an important role during development, regulating cell differentiation, proliferation and survival. (mit.edu)
  • First, we explored the ability of integrins to transmit mechanical signals in an in vitro model of tendon differentiation to explore whether integrins play a role in mechanotransduction at an early stage in the life of a tendon cell. (nature.com)
  • Because the initial critical step in ovarian cancer metastasis is the attachment of cancer cells to the peritoneum or omentum and because clinical trials have provided positive results for anti-angiogenic therapy, therapies targeting integrins may be the most feasible approach for treating cancer. (mdpi.com)
  • This review summarizes the current understanding of integrin biology in ovarian cancer metastasis and various therapeutic approaches involving integrin inhibitors. (mdpi.com)
  • Because of their central role in mediating invasion and metastasis, integrins are likely to be useful biomarkers. (onmedica.com)
  • In this study, we discovered that mechanical stimulation of integrin β1 leads to the phosphorylation of AKT, an event which required the presence of integrin-linked kinase (ILK). (nature.com)
  • Caveolin-1 (Cav-1) associates with integrins and Fyn (Src-related kinase), where Cav-1 mediates the phosphorylation of Shc by Fyn. (thermofisher.com)
  • Functional domains of integrin-linked kinase (ILK). (atlasgeneticsoncology.org)
  • The integrin-binding site is in the extreme C-terminus of the kinase domain (residues 293-451) (Diagram author: Elena Serrano). (atlasgeneticsoncology.org)
  • Integrin-linked kinase (ILK) (Hannigan et al. (atlasgeneticsoncology.org)
  • 2013). The kinase activity of ILK is stimulated by integrins and soluble mediators, including growth factors and chemokines, and is regulated in a phosphoinositide 3-kinase (PI3K)-dependent manner. (atlasgeneticsoncology.org)
  • Integrins carrying this domain either bind to collagens (e.g. integrins α1 β1, and α2 β1), or act as cell-cell adhesion molecules (integrins of the β2 family). (wikipedia.org)
  • These include coxsackievirus A9 (CV-A9), echovirus 9, and human parechovirus 1 that are among the most common and epidemic human picornaviruses and bind to α V-integrins via RGD motif that resides on virus capsid. (hindawi.com)
  • Two of these, talin and kindlin, bind directly to the cytoplasmic domain of the integrin β subunit and are key regulators of integrin affinity ("inside-out" activation). (abcam.com)
  • The β2 integrins LFA-1 and Mac-1 (CD11a/CD18 and CD11b/CD18) bind to ICAM1, and the β1 integrins (such as VLA-4) bind VCAM1 . (humpath.com)
  • Integrins are known to participate in cell adhesion as well as cell-surface-mediated signaling. (wikipedia.org)
  • Integrin expression may also be regulated by miRNAs, which can also modulate integrin signaling and function. (nih.gov)
  • Together, these findings indicate that seizures activate integrin signaling and induce a turnover in adhesive contacts and that both processes contribute to lasting changes in circuit and synaptic function underlying epileptogenesis. (springer.com)
  • however, a CD98hc mutant that interacts with β1 integrins, but not CD98 light chains, restored integrin-dependent signaling and protection from apoptosis. (pnas.org)
  • The downward movements of the C-terminal helices of the alpha I and beta I domains and the swing-out of the hybrid domain play pivotal roles in integrin conformational signaling. (nih.gov)
  • We propose that the strength of adhesive signaling from integrins influences the threshold of response by growth cones to repellent axon guidance cues. (jneurosci.org)
  • Integrin adhesion or signaling may intersect with growth cone guidance signals at pathfinding choice points. (jneurosci.org)
  • Rab11b-mediated control of integrin β1 surface expression allows efficient engagement with the brain ECM, activating mechanotransduction signaling to promote survival. (nature.com)
  • Only anti-β3 integrin-mediated FNAIT reduced brain and retina vessel density, impaired angiogenic signaling, and increased endothelial cell apoptosis, all of which were abrogated by maternal administration of intravenous immunoglobulin (IVIG). (jci.org)
  • Not only do integrins perform this outside-in signaling, but they also operate an inside-out mode. (wikidoc.org)
  • The tendency of these cancers to metastasize to bone and induce bone destruction is, in part, due to alterations in integrin expression and signaling. (mdpi.com)
  • Activation of PI3K links integrin activation with the Akt signaling pathway for activation of cell survival mechanisms. (thermofisher.com)
  • Another signaling pathway utilized by integrin for MAPK activation is via integrin association with caveolins. (thermofisher.com)
  • A number of different assays are available to detect cellular adhesion and the cellular processes influenced by integrin signaling mechanisms. (thermofisher.com)
  • No major differences between signaling via alpha 5 beta 1 or alpha v beta 3 integrins were detected. (diva-portal.org)
  • Integrin signaling is bidirectional. (humpath.com)
  • Hynes RO (1992) Integrins: versatility, modulation, and signaling in cell adhesion. (springer.com)
  • Integrins, angiogenesis and vascu. (mendeley.com)
  • A recent paper by Vitorino et al ([2015][1]) defined a new MAP4K4-moesin-talin-β1‐integrin pathway that could be therapeutically exploited to suppress pathologic angiogenesis. (mendeley.com)
  • ICH and impairment of retinal angiogenesis were further reproduced in neonates by injection of anti-β3 integrin, but not anti-GPIbα antisera. (jci.org)
  • Preclinical studies have shown the effectiveness of several integrin inhibitors for blocking cancer progression, especially by blocking angiogenesis. (mdpi.com)
  • To get novel insights about the antiangiogenic activity of resveratrol, we compared cis - and trans -resveratrol stereoisomers for their effect on the angiogenesis process and endothelial α v β 3 integrin function. (aacrjournals.org)
  • Synthetic peptide conjugated to KLH corresponding to C terminal residues of Human Integrin alpha V (NP_002201.1). (abcam.com)
  • ITGA11 shows 86% identity with mouse integrin α11 at the nucleotide level, translating to 89% identity at the amino-acid level. (atlasgeneticsoncology.org)
  • I read with interest the study by De Archangelis et al 1 on the protective role of hemidesmosomes against colitis and colorectal cancer using genetically modified mouse integrin α6 subunit mutant models. (bmj.com)
  • Integrins interact with ECM components via their extracellular domains, while their cytoplasmic domains play a pivotal role in mediating integrin-dependent cellular functions. (humpath.com)
  • These data establish an important role for CD98hc in integrin-dependent signals that contribute to tumorigenesis. (pnas.org)
  • Experiments have also shown that integrins transmit bidirectional signals across the plasma membrane by coupling extracellular conformational change with an unclasping and separation of the alpha and beta transmembrane and cytoplasmic domains. (nih.gov)
  • Integrins also are involved in transforming or translating cell signals. (biologynews.net)
  • Cells are exposed to several types of integrin stimuli, which generate responses generally referred to as "integrin signals", but the specific responses to different integrin stimuli are poorly defined. (diva-portal.org)
  • In conclusion, the results showed that "integrin signals" are composedof separate sets of reactions triggered by different types of integrin stimulation. (diva-portal.org)
  • Instead, CD98hc was involved in the cell's ability to exert force on the matrix and did so by dint of its capacity to interact with integrins to support downstream signals that lead to activation of RhoA small GTPase. (sigmaaldrich.com)
  • Thus, alterations in integrin expression profiles allow cells to modulate several critical aspects of the motile machinery through Rho GTPases. (rupress.org)
  • Integrins are cell adhesion molecules that play critical roles in development, wound healing, hemostasis, immunity and cancer. (nih.gov)
  • The two, integrin alpha1 beta1 and integrin alpha2 beta1, are members of a class of molecules that are involved in attaching cells to other cells and to the extracellular matrix (a part of tissue that is not part of any cell). (biologynews.net)
  • Cell Adhesion Molecules, and introducing the Integrins. (ubc.ca)
  • The β1-containing integrins (ITGB1) are also called VLA molecules, referring to "very late activation" molecules, because α1β1 and α2β1 were first shown to be expressed on T cells 2 to 4 weeks after repetitive stimulation in vitro. (humpath.com)
  • Helper T cells are recruited to sites of inflammation by the coordinated expression of several homing molecules, including integrins ( 1 , 2 ). (jimmunol.org)
  • Knockouts of most other αv-associated β integrins (β3, β5, and β6) are viable and reveal roles for αv in repair of tissue injury, inflammation, and immune responses ( 1 , 6 - 8 ). (pnas.org)
  • Deletion of alpha4 integrins from adult hematopoietic cells reveals roles in homeostasis, regeneration, and homing. (semanticscholar.org)
  • Thus, one of the roles of the PS integrins in late Drosophila development is to ensure the correct apposition and patterning of the wing epithelia. (biologists.org)
  • Our data suggest that transformed proliferating cells from HCC are more sensitive to knockdown of integrins than normal quiescent hepatocytes, highlighting the potential of small interfering RNA-mediated inhibition of integrins as an anti-cancer therapeutic approach. (mit.edu)
  • Applied Integrin Sciences, Inc. announced today that its first cancer therapeutic drug candidate eliminated all intraperitoneal ovarian cancer tumors in a pre-clinical study testing a novel treatment regimen. (news-medical.net)
  • The results presented in this paper set the stage for the advancement of integrin α v β 3 -targeted NSs as therapeutic nanoconstructs for effective cancer therapy. (dovepress.com)
  • Thus, murine Th1 and Th2 cells use distinct integrins for homing, suggesting novel opportunities for integrin-based therapeutic intervention in diverse human ailments influenced by Th2 cells. (jimmunol.org)
  • The integrin knockdown leads to significant retardation of HCC progression, reducing proliferation and increasing tumour cell death. (mit.edu)
  • The cytoplasmic domains of integrins play a pivotal role in integrin function. (biologists.org)
  • Finally, we analysed the importance of specific amino acids of the intracellular domain of beta1 integrin in keratinocytes in vivo by generating 8 mice strains which in skin express only point or deletion mutants of beta1 integrin. (uni-muenchen.de)
  • Moreover, intraperitoneal injection of the α V integrin blocking peptide cRGDfV (Calbiochem, United Kingdom) increased the growth response and also greatly reduced the calculated remodeling index. (ahajournals.org)
  • The mature α11 peptide is 1166-amino acid-long (M.W. 145 kDa in SDS-PAGE under non-reducing conditions), which is longer than any other currently identified integrin α-chain (with the closest being αE, which is composed of 1160 amino acids). (atlasgeneticsoncology.org)
  • Axon growth may be facilitated by a low density of integrin adhesive contacts that can be formed and broken easily. (jneurosci.org)
  • In vitro, β 2 integrin-mediated adhesion of leukocytes to endothelium was lost upon removal of uPAR from the leukocyte surface by phosphatidyl-inositol-specific phospholipase C. Leukocyte adhesion was reconstituted when soluble intact uPAR, but not a truncated form lacking the uPA-binding domain, was allowed to reassociate with the cell surface. (rupress.org)
  • In acute inflammation, LFA-1 and Mac-1 are the predominant β 2 integrins mediating leukocyte adhesion to vascular endothelium. (rupress.org)
  • As summarized in Fig. 1, only certain combinations of integrins are observed: major groupings include integrins of the β1 subfamily and integrins containing the αv subunit. (springer.com)
  • Determining the precise contributions of αv integrins to immune functions in vivo has been limited by the lethal phenotype of αv knockout mice, which die from vascular and developmental defects ( 2 ). (pnas.org)
  • To test this model in vivo, we mutated the integrin binding site of PDE4D5 in mice. (jci.org)
  • In vivo, the β 2 integrin-dependent recruitment of leukocytes to the inflamed peritoneum of uPAR-deficient mice was significantly reduced as compared with wild-type animals. (rupress.org)
  • We demonstrated now, that fourfold reduction of beta1 integrin expression in keratinocytes in vivo did not impair wound healing. (uni-muenchen.de)
  • i ) It can associate with and regulate the function of selected integrins ( 5 - 8 ), and ( ii ) it can regulate the expression and distribution of the light chains to modulate amino acid transport function ( 3 ). (pnas.org)
  • Hogg N , Patzak I and Willenbrock F (2011) The insider's guide to leukocyte integrin signalling and function. (els.net)
  • This is the premier international conference for academic, government and industry scientists interested in understanding how integrins and the extracellular matrix regulate virtually every aspect of cell and tissue function. (grc.org)
  • We believe these results may have widespread consequences for the control of cell function by integrins. (jci.org)
  • conditional deletion of Dnm2 in mouse embryos causes severe angiogenic phenotypes that are consistent with impaired integrin function. (biologists.org)
  • The cells were divided into control, SBA treated groups, integrin inhibitor groups, and SBA + integrin inhibitor groups to determine the integrin function in SCA. (mdpi.com)
  • In this paper we use viable mutations in integrin component genes to look at integrin function in the later stages of development of one adult structure, the wing. (biologists.org)
  • The β2 integrins are also called CD11a-cCD18, or the leukocyte function-associated antigen-1 (LFA-1) family, CD11a-c referring to different α chains and CD18 to the common β2 subunit. (humpath.com)
  • Recently, trans -resveratrol has been shown to interact with the β 3 integrin subunit, raising the possibility that inhibition of endothelial α v β 3 integrin function may concur to its angiosuppressive activity. (aacrjournals.org)
  • The different antiangiogenic potential of resveratrol stereoisomers is related, at least in part, to their different capacity to affect α v β 3 integrin function. (aacrjournals.org)
  • Jones, Kurpakus, Cooper, Quaranta: A function for the integrin alpha 6 beta 4 in the hemidesmosome. (antikoerper-online.de)
  • I use Xenopus laevis as my experimental model system to study GIPC-regulated integrin function. (uwaterloo.ca)
  • Together, my data indicate a critical role for XGIPC in modulating α5β1 integrin function during early embryonic morphogenesis. (uwaterloo.ca)
  • CD61 is a 90-110 kDa member of the beta integrin family expressed by a wide variety of cells, including leukocytes, platelets, endothelial and smooth muscle cells. (fishersci.com)