Intracellular messenger formed by the action of phospholipase C on phosphatidylinositol 4,5-bisphosphate, which is one of the phospholipids that make up the cell membrane. Inositol 1,4,5-trisphosphate is released into the cytoplasm where it releases calcium ions from internal stores within the cell's endoplasmic reticulum. These calcium ions stimulate the activity of B kinase or calmodulin.
Intracellular receptors that bind to INOSITOL 1,4,5-TRISPHOSPHATE and play an important role in its intracellular signaling. Inositol 1,4,5-trisphosphate receptors are calcium channels that release CALCIUM in response to increased levels of inositol 1,4,5-trisphosphate in the CYTOPLASM.
Phosphoric acid esters of inositol. They include mono- and polyphosphoric acid esters, with the exception of inositol hexaphosphate which is PHYTIC ACID.
An isomer of glucose that has traditionally been considered to be a B vitamin although it has an uncertain status as a vitamin and a deficiency syndrome has not been identified in man. (From Martindale, The Extra Pharmacopoeia, 30th ed, p1379) Inositol phospholipids are important in signal transduction.
A basic element found in nearly all organized tissues. It is a member of the alkaline earth family of metals with the atomic symbol Ca, atomic number 20, and atomic weight 40. Calcium is the most abundant mineral in the body and combines with phosphorus to form calcium phosphate in the bones and teeth. It is essential for the normal functioning of nerves and muscles and plays a role in blood coagulation (as factor IV) and in many enzymatic processes.
Voltage-dependent cell membrane glycoproteins selectively permeable to calcium ions. They are categorized as L-, T-, N-, P-, Q-, and R-types based on the activation and inactivation kinetics, ion specificity, and sensitivity to drugs and toxins. The L- and T-types are present throughout the cardiovascular and central nervous systems and the N-, P-, Q-, & R-types are located in neuronal tissue.
Derivatives of phosphatidic acids in which the phosphoric acid is bound in ester linkage to the hexahydroxy alcohol, myo-inositol. Complete hydrolysis yields 1 mole of glycerol, phosphoric acid, myo-inositol, and 2 moles of fatty acids.
Intracellular receptors that can be found in the cytoplasm or in the nucleus. They bind to extracellular signaling molecules that migrate through or are transported across the CELL MEMBRANE. Many members of this class of receptors occur in the cytoplasm and are transported to the CELL NUCLEUS upon ligand-binding where they signal via DNA-binding and transcription regulation. Also included in this category are receptors found on INTRACELLULAR MEMBRANES that act via mechanisms similar to CELL SURFACE RECEPTORS.
Phosphatidylinositols in which one or more alcohol group of the inositol has been substituted with a phosphate group.
A subclass of phospholipases that hydrolyze the phosphoester bond found in the third position of GLYCEROPHOSPHOLIPIDS. Although the singular term phospholipase C specifically refers to an enzyme that catalyzes the hydrolysis of PHOSPHATIDYLCHOLINE (EC 3.1.4.3), it is commonly used in the literature to refer to broad variety of enzymes that specifically catalyze the hydrolysis of PHOSPHATIDYLINOSITOLS.
The type species of ALPHAVIRUS normally transmitted to birds by CULEX mosquitoes in Egypt, South Africa, India, Malaya, the Philippines, and Australia. It may be associated with fever in humans. Serotypes (differing by less than 17% in nucleotide sequence) include Babanki, Kyzylagach, and Ockelbo viruses.
Signal transduction mechanisms whereby calcium mobilization (from outside the cell or from intracellular storage pools) to the cytoplasm is triggered by external stimuli. Calcium signals are often seen to propagate as waves, oscillations, spikes, sparks, or puffs. The calcium acts as an intracellular messenger by activating calcium-responsive proteins.
A group of enzymes that transfers a phosphate group onto an alcohol group acceptor. EC 2.7.1.
Complexing agent for removal of traces of heavy metal ions. It acts also as a hypocalcemic agent.
A phosphoinositide present in all eukaryotic cells, particularly in the plasma membrane. It is the major substrate for receptor-stimulated phosphoinositidase C, with the consequent formation of inositol 1,4,5-triphosphate and diacylglycerol, and probably also for receptor-stimulated inositol phospholipid 3-kinase. (Kendrew, The Encyclopedia of Molecular Biology, 1994)
The rate dynamics in chemical or physical systems.
Systems in which an intracellular signal is generated in response to an intercellular primary messenger such as a hormone or neurotransmitter. They are intermediate signals in cellular processes such as metabolism, secretion, contraction, phototransduction, and cell growth. Examples of second messenger systems are the adenyl cyclase-cyclic AMP system, the phosphatidylinositol diphosphate-inositol triphosphate system, and the cyclic GMP system.
Cyclic compounds with a ring size of approximately 1-4 dozen atoms.
An element in the alkali metals family. It has the atomic symbol Li, atomic number 3, and atomic weight [6.938; 6.997]. Salts of lithium are used in treating BIPOLAR DISORDER.
A slowly hydrolyzed CHOLINERGIC AGONIST that acts at both MUSCARINIC RECEPTORS and NICOTINIC RECEPTORS.
Inosine 5'-(tetrahydrogen triphosphate). An inosine nucleotide containing three phosphate groups esterified to the sugar moiety. Synonym: IRPPP.
A sesquiterpene lactone found in roots of THAPSIA. It inhibits CA(2+)-TRANSPORTING ATPASE mediated uptake of CALCIUM into SARCOPLASMIC RETICULUM.
An ethylmercury-sulfidobenzoate that has been used as a preservative in VACCINES; ANTIVENINS; and OINTMENTS. It was formerly used as a topical antiseptic. It degrades to ethylmercury and thiosalicylate.
The intracellular transfer of information (biological activation/inhibition) through a signal pathway. In each signal transduction system, an activation/inhibition signal from a biologically active molecule (hormone, neurotransmitter) is mediated via the coupling of a receptor/enzyme to a second messenger system or to an ion channel. Signal transduction plays an important role in activating cellular functions, cell differentiation, and cell proliferation. Examples of signal transduction systems are the GAMMA-AMINOBUTYRIC ACID-postsynaptic receptor-calcium ion channel system, the receptor-mediated T-cell activation pathway, and the receptor-mediated activation of phospholipases. Those coupled to membrane depolarization or intracellular release of calcium include the receptor-mediated activation of cytotoxic functions in granulocytes and the synaptic potentiation of protein kinase activation. Some signal transduction pathways may be part of larger signal transduction pathways; for example, protein kinase activation is part of the platelet activation signal pathway.
Inorganic or organic compounds that contain boron as an integral part of the molecule.
Intracellular fluid from the cytoplasm after removal of ORGANELLES and other insoluble cytoplasmic components.
Unsaturated derivatives of the ESTRANES with methyl groups at carbon-13, with no carbon at carbon-10, and with no more than one carbon at carbon-17. They must contain one or more double bonds.
A fluorescent calcium chelating agent which is used to study intracellular calcium in tissues.
Established cell cultures that have the potential to propagate indefinitely.
A group of compounds that are derivatives of oxo-pyrrolidines. A member of this group is 2-oxo pyrrolidine, which is an intermediate in the manufacture of polyvinylpyrrolidone. (From Merck Index, 11th ed)
A group of enzymes that catalyzes the transfer of a phosphate group onto a phosphate group acceptor. EC 2.7.4.
A pyridine nucleotide that mobilizes CALCIUM. It is synthesized from nicotinamide adenine dinucleotide (NAD) by ADP RIBOSE CYCLASE.
A chelating agent relatively more specific for calcium and less toxic than EDETIC ACID.
The lipid- and protein-containing, selectively permeable membrane that surrounds the cytoplasm in prokaryotic and eukaryotic cells.
Liquid chromatographic techniques which feature high inlet pressures, high sensitivity, and high speed.
Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others.
A system of cisternae in the CYTOPLASM of many cells. In places the endoplasmic reticulum is continuous with the plasma membrane (CELL MEMBRANE) or outer membrane of the nuclear envelope. If the outer surfaces of the endoplasmic reticulum membranes are coated with ribosomes, the endoplasmic reticulum is said to be rough-surfaced (ENDOPLASMIC RETICULUM, ROUGH); otherwise it is said to be smooth-surfaced (ENDOPLASMIC RETICULUM, SMOOTH). (King & Stansfield, A Dictionary of Genetics, 4th ed)
The largest of the three pairs of SALIVARY GLANDS. They lie on the sides of the FACE immediately below and in front of the EAR.
A tetrameric calcium release channel in the SARCOPLASMIC RETICULUM membrane of SMOOTH MUSCLE CELLS, acting oppositely to SARCOPLASMIC RETICULUM CALCIUM-TRANSPORTING ATPASES. It is important in skeletal and cardiac excitation-contraction coupling and studied by using RYANODINE. Abnormalities are implicated in CARDIAC ARRHYTHMIAS and MUSCULAR DISEASES.
A quality of cell membranes which permits the passage of solvents and solutes into and out of cells.
The part of brain that lies behind the BRAIN STEM in the posterior base of skull (CRANIAL FOSSA, POSTERIOR). It is also known as the "little brain" with convolutions similar to those of CEREBRAL CORTEX, inner white matter, and deep cerebellar nuclei. Its function is to coordinate voluntary movements, maintain balance, and learn motor skills.
A divalent calcium ionophore that is widely used as a tool to investigate the role of intracellular calcium in cellular processes.
The process of cleaving a chemical compound by the addition of a molecule of water.
A phosphoinositide phospholipase C subtype that is structurally defined by the presence of an N-terminal pleckstrin-homology and EF-hand domains, a central catalytic domain, and a C-terminal calcium-dependent membrane-binding domain.
A rather large group of enzymes comprising not only those transferring phosphate but also diphosphate, nucleotidyl residues, and others. These have also been subdivided according to the acceptor group. (From Enzyme Nomenclature, 1992) EC 2.7.
A highly anionic organic phosphate which is present in human red blood cells at about the same molar ratio as hemoglobin. It binds to deoxyhemoglobin but not the oxygenated form, therefore diminishing the oxygen affinity of hemoglobin. This is essential in enabling hemoglobin to unload oxygen in tissue capillaries. It is also an intermediate in the conversion of 3-phosphoglycerate to 2-phosphoglycerate by phosphoglycerate mutase (EC 5.4.2.1). (From Stryer Biochemistry, 4th ed, p160; Enzyme Nomenclature, 1992, p508)
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
A methylpyrrole-carboxylate from RYANIA that disrupts the RYANODINE RECEPTOR CALCIUM RELEASE CHANNEL to modify CALCIUM release from SARCOPLASMIC RETICULUM resulting in alteration of MUSCLE CONTRACTION. It was previously used in INSECTICIDES. It is used experimentally in conjunction with THAPSIGARGIN and other inhibitors of CALCIUM ATPASE uptake of calcium into SARCOPLASMIC RETICULUM.
A methylxanthine naturally occurring in some beverages and also used as a pharmacological agent. Caffeine's most notable pharmacological effect is as a central nervous system stimulant, increasing alertness and producing agitation. It also relaxes SMOOTH MUSCLE, stimulates CARDIAC MUSCLE, stimulates DIURESIS, and appears to be useful in the treatment of some types of headache. Several cellular actions of caffeine have been observed, but it is not entirely clear how each contributes to its pharmacological profile. Among the most important are inhibition of cyclic nucleotide PHOSPHODIESTERASES, antagonism of ADENOSINE RECEPTORS, and modulation of intracellular calcium handling.
Conversion of an inactive form of an enzyme to one possessing metabolic activity. It includes 1, activation by ions (activators); 2, activation by cofactors (coenzymes); and 3, conversion of an enzyme precursor (proenzyme or zymogen) to an active enzyme.
Artifactual vesicles formed from the endoplasmic reticulum when cells are disrupted. They are isolated by differential centrifugation and are composed of three structural features: rough vesicles, smooth vesicles, and ribosomes. Numerous enzyme activities are associated with the microsomal fraction. (Glick, Glossary of Biochemistry and Molecular Biology, 1990; from Rieger et al., Glossary of Genetics: Classical and Molecular, 5th ed)
The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.
An serine-threonine protein kinase that requires the presence of physiological concentrations of CALCIUM and membrane PHOSPHOLIPIDS. The additional presence of DIACYLGLYCEROLS markedly increases its sensitivity to both calcium and phospholipids. The sensitivity of the enzyme can also be increased by PHORBOL ESTERS and it is believed that protein kinase C is the receptor protein of tumor-promoting phorbol esters.
A set of BACTERIAL ADHESINS and TOXINS, BIOLOGICAL produced by BORDETELLA organisms that determine the pathogenesis of BORDETELLA INFECTIONS, such as WHOOPING COUGH. They include filamentous hemagglutinin; FIMBRIAE PROTEINS; pertactin; PERTUSSIS TOXIN; ADENYLATE CYCLASE TOXIN; dermonecrotic toxin; tracheal cytotoxin; Bordetella LIPOPOLYSACCHARIDES; and tracheal colonization factor.
The introduction of a phosphoryl group into a compound through the formation of an ester bond between the compound and a phosphorus moiety.
One of the virulence factors produced by BORDETELLA PERTUSSIS. It is a multimeric protein composed of five subunits S1 - S5. S1 contains mono ADPribose transferase activity.
An adenine nucleotide containing three phosphate groups esterified to the sugar moiety. In addition to its crucial roles in metabolism adenosine triphosphate is a neurotransmitter.
Regulatory proteins that act as molecular switches. They control a wide range of biological processes including: receptor signaling, intracellular signal transduction pathways, and protein synthesis. Their activity is regulated by factors that control their ability to bind to and hydrolyze GTP to GDP. EC 3.6.1.-.
Nucleotides in which the base moiety is substituted with one or more sulfur atoms.
A type C phospholipase with specificity towards PHOSPHATIDYLINOSITOLS that contain INOSITOL 1,4,5-TRISPHOSPHATE. Many of the enzymes listed under this classification are involved in intracellular signaling.
A chemotherapeutic agent that acts against erythrocytic forms of malarial parasites. Hydroxychloroquine appears to concentrate in food vacuoles of affected protozoa. It inhibits plasmodial heme polymerase. (From Gilman et al., Goodman and Gilman's The Pharmacological Basis of Therapeutics, 9th ed, p970)
Cell surface proteins that bind signalling molecules external to the cell with high affinity and convert this extracellular event into one or more intracellular signals that alter the behavior of the target cell (From Alberts, Molecular Biology of the Cell, 2nd ed, pp693-5). Cell surface receptors, unlike enzymes, do not chemically alter their ligands.
A phosphoinositide phospholipase C subtype that is primarily regulated by PROTEIN-TYROSINE KINASES. It is structurally related to PHOSPHOLIPASE C DELTA with the addition of SRC HOMOLOGY DOMAINS and pleckstrin homology domains located between two halves of the CATALYTIC DOMAIN.
One of the two major classes of cholinergic receptors. Muscarinic receptors were originally defined by their preference for MUSCARINE over NICOTINE. There are several subtypes (usually M1, M2, M3....) that are characterized by their cellular actions, pharmacology, and molecular biology.
A family of winged insects of the suborder HETEROPTERA, called assassin bugs, because most prey on other insects. However one subfamily, TRIATOMINAE, attacks humans and other vertebrates and transmits Chagas disease.
The fluid inside CELLS.
The relationship between the dose of an administered drug and the response of the organism to the drug.
Phosphotransferases that catalyzes the conversion of 1-phosphatidylinositol to 1-phosphatidylinositol 3-phosphate. Many members of this enzyme class are involved in RECEPTOR MEDIATED SIGNAL TRANSDUCTION and regulation of vesicular transport with the cell. Phosphatidylinositol 3-Kinases have been classified both according to their substrate specificity and their mode of action within the cell.
An adenine nucleotide containing one phosphate group which is esterified to both the 3'- and 5'-positions of the sugar moiety. It is a second messenger and a key intracellular regulator, functioning as a mediator of activity for a number of hormones, including epinephrine, glucagon, and ACTH.
Antibiotic complex produced by Streptomyces fradiae. It is composed of neomycins A, B, and C. It acts by inhibiting translation during protein synthesis.
A class of compounds composed of repeating 5-carbon units of HEMITERPENES.
A phosphoinositide phospholipase C subtype that is primarily regulated by its association with HETEROTRIMERIC G-PROTEINS. It is structurally related to PHOSPHOLIPASE C DELTA with the addition of C-terminal extension of 400 residues.
CELL LINES derived from the CV-1 cell line by transformation with a replication origin defective mutant of SV40 VIRUS, which codes for wild type large T antigen (ANTIGENS, POLYOMAVIRUS TRANSFORMING). They are used for transfection and cloning. (The CV-1 cell line was derived from the kidney of an adult male African green monkey (CERCOPITHECUS AETHIOPS).)
The process in which substances, either endogenous or exogenous, bind to proteins, peptides, enzymes, protein precursors, or allied compounds. Specific protein-binding measures are often used as assays in diagnostic assessments.
An enzyme that catalyzes the formation of myo-inositol-1-phosphate from glucose-6-phosphate in the presence of NAD. EC 5.5.1.4.
A type of glycoside widely distributed in plants. Each consists of a sapogenin as the aglycone moiety, and a sugar. The sapogenin may be a steroid or a triterpene and the sugar may be glucose, galactose, a pentose, or a methylpentose.
A phosphorus-oxygen lyase found primarily in BACTERIA. The enzyme catalyzes the cleavage of a phosphoester linkage in 1-phosphatidyl-1D-myo-inositol to form 1D-myo-inositol 1,2-cyclic phosphate and diacylglycerol. The enzyme was formerly classified as a phosphoric diester hydrolase (EC 3.1.4.10) and is often referred to as a TYPE C PHOSPHOLIPASES. However it is now known that a cyclic phosphate is the final product of this enzyme and that water does not enter into the reaction.
Fatty acid derivatives of glycerophosphates. They are composed of glycerol bound in ester linkage with 1 mole of phosphoric acid at the terminal 3-hydroxyl group and with 2 moles of fatty acids at the other two hydroxyl groups.
Structurally related forms of an enzyme. Each isoenzyme has the same mechanism and classification, but differs in its chemical, physical, or immunological characteristics.
Cation-transporting proteins that utilize the energy of ATP hydrolysis for the transport of CALCIUM. They differ from CALCIUM CHANNELS which allow calcium to pass through a membrane without the use of energy.
A salt of lithium that has been used experimentally as an immunomodulator.
Chemical bond cleavage reactions resulting from absorption of radiant energy.
The phenomenon whereby certain chemical compounds have structures that are different although the compounds possess the same elemental composition. (From McGraw-Hill Dictionary of Scientific and Technical Terms, 5th ed)
Female germ cells derived from OOGONIA and termed OOCYTES when they enter MEIOSIS. The primary oocytes begin meiosis but are arrested at the diplotene state until OVULATION at PUBERTY to give rise to haploid secondary oocytes or ova (OVUM).
Compounds or agents that combine with an enzyme in such a manner as to prevent the normal substrate-enzyme combination and the catalytic reaction.
Unstable isotopes of phosphorus that decay or disintegrate emitting radiation. P atoms with atomic weights 28-34 except 31 are radioactive phosphorus isotopes.
Guanosine 5'-(tetrahydrogen triphosphate). A guanine nucleotide containing three phosphate groups esterified to the sugar moiety.
Genetically identical individuals developed from brother and sister matings which have been carried out for twenty or more generations or by parent x offspring matings carried out with certain restrictions. This also includes animals with a long history of closed colony breeding.
A nonapeptide messenger that is enzymatically produced from KALLIDIN in the blood where it is a potent but short-lived agent of arteriolar dilation and increased capillary permeability. Bradykinin is also released from MAST CELLS during asthma attacks, from gut walls as a gastrointestinal vasodilator, from damaged tissues as a pain signal, and may be a neurotransmitter.
Unstable isotopes of calcium that decay or disintegrate emitting radiation. Ca atoms with atomic weights 39, 41, 45, 47, 49, and 50 are radioactive calcium isotopes.
A light microscopic technique in which only a small spot is illuminated and observed at a time. An image is constructed through point-by-point scanning of the field in this manner. Light sources may be conventional or laser, and fluorescence or transmitted observations are possible.
Elements of limited time intervals, contributing to particular results or situations.
Domesticated bovine animals of the genus Bos, usually kept on a farm or ranch and used for the production of meat or dairy products or for heavy labor.
A mature haploid female germ cell extruded from the OVARY at OVULATION.
A subgroup of TRP cation channels that contain 3-4 ANKYRIN REPEAT DOMAINS and a conserved C-terminal domain. Members are highly expressed in the CENTRAL NERVOUS SYSTEM. Selectivity for calcium over sodium ranges from 0.5 to 10.
Chemicals that bind to and remove ions from solutions. Many chelating agents function through the formation of COORDINATION COMPLEXES with METALS.
Agents that emit light after excitation by light. The wave length of the emitted light is usually longer than that of the incident light. Fluorochromes are substances that cause fluorescence in other substances, i.e., dyes used to mark or label other compounds with fluorescent tags.
A highly acidic mucopolysaccharide formed of equal parts of sulfated D-glucosamine and D-glucuronic acid with sulfaminic bridges. The molecular weight ranges from six to twenty thousand. Heparin occurs in and is obtained from liver, lung, mast cells, etc., of vertebrates. Its function is unknown, but it is used to prevent blood clotting in vivo and vitro, in the form of many different salts.
Compounds that contain a BENZENE ring fused to a furan ring.
An ester formed between the aldehydic carbon of RIBOSE and the terminal phosphate of ADENOSINE DIPHOSPHATE. It is produced by the hydrolysis of nicotinamide-adenine dinucleotide (NAD) by a variety of enzymes, some of which transfer an ADP-ribosyl group to target proteins.
The parts of a macromolecule that directly participate in its specific combination with another molecule.
A nodular organ in the ABDOMEN that contains a mixture of ENDOCRINE GLANDS and EXOCRINE GLANDS. The small endocrine portion consists of the ISLETS OF LANGERHANS secreting a number of hormones into the blood stream. The large exocrine portion (EXOCRINE PANCREAS) is a compound acinar gland that secretes several digestive enzymes into the pancreatic ductal system that empties into the DUODENUM.
A tetradecapeptide originally obtained from the skins of toads Bombina bombina and B. variegata. It is also an endogenous neurotransmitter in many animals including mammals. Bombesin affects vascular and other smooth muscle, gastric secretion, and renal circulation and function.
The voltage differences across a membrane. For cellular membranes they are computed by subtracting the voltage measured outside the membrane from the voltage measured inside the membrane. They result from differences of inside versus outside concentration of potassium, sodium, chloride, and other ions across cells' or ORGANELLES membranes. For excitable cells, the resting membrane potentials range between -30 and -100 millivolts. Physical, chemical, or electrical stimuli can make a membrane potential more negative (hyperpolarization), or less negative (depolarization).
Unstriated and unstriped muscle, one of the muscles of the internal organs, blood vessels, hair follicles, etc. Contractile elements are elongated, usually spindle-shaped cells with centrally located nuclei. Smooth muscle fibers are bound together into sheets or bundles by reticular fibers and frequently elastic nets are also abundant. (From Stedman, 25th ed)
Theoretical representations that simulate the behavior or activity of biological processes or diseases. For disease models in living animals, DISEASE MODELS, ANIMAL is available. Biological models include the use of mathematical equations, computers, and other electronic equipment.
Proteins prepared by recombinant DNA technology.
The uptake of naked or purified DNA by CELLS, usually meaning the process as it occurs in eukaryotic cells. It is analogous to bacterial transformation (TRANSFORMATION, BACTERIAL) and both are routinely employed in GENE TRANSFER TECHNIQUES.
The level of protein structure in which combinations of secondary protein structures (alpha helices, beta sheets, loop regions, and motifs) pack together to form folded shapes called domains. Disulfide bridges between cysteines in two different parts of the polypeptide chain along with other interactions between the chains play a role in the formation and stabilization of tertiary structure. Small proteins usually consist of only one domain but larger proteins may contain a number of domains connected by segments of polypeptide chain which lack regular secondary structure.
A class of enzymes that catalyze the hydrolysis of one of the two ester bonds in a phosphodiester compound. EC 3.1.4.
A heat-stable, low-molecular-weight activator protein found mainly in the brain and heart. The binding of calcium ions to this protein allows this protein to bind to cyclic nucleotide phosphodiesterases and to adenyl cyclase with subsequent activation. Thereby this protein modulates cyclic AMP and cyclic GMP levels.
GLYCEROL esterified with FATTY ACIDS.
The measurement of frequency or oscillation changes.
Compounds containing carbon-phosphorus bonds in which the phosphorus component is also bonded to one or more sulfur atoms. Many of these compounds function as CHOLINERGIC AGENTS and as INSECTICIDES.
The opening and closing of ion channels due to a stimulus. The stimulus can be a change in membrane potential (voltage-gated), drugs or chemical transmitters (ligand-gated), or a mechanical deformation. Gating is thought to involve conformational changes of the ion channel which alters selective permeability.
Agents that increase calcium influx into calcium channels of excitable tissues. This causes vasoconstriction in VASCULAR SMOOTH MUSCLE and/or CARDIAC MUSCLE cells as well as stimulation of insulin release from pancreatic islets. Therefore, tissue-selective calcium agonists have the potential to combat cardiac failure and endocrinological disorders. They have been used primarily in experimental studies in cell and tissue culture.
Lipids containing one or more phosphate groups, particularly those derived from either glycerol (phosphoglycerides see GLYCEROPHOSPHOLIPIDS) or sphingosine (SPHINGOLIPIDS). They are polar lipids that are of great importance for the structure and function of cell membranes and are the most abundant of membrane lipids, although not stored in large amounts in the system.
Non-nucleated disk-shaped cells formed in the megakaryocyte and found in the blood of all mammals. They are mainly involved in blood coagulation.
A phorbol ester found in CROTON OIL with very effective tumor promoting activity. It stimulates the synthesis of both DNA and RNA.
Antidiuretic hormones released by the NEUROHYPOPHYSIS of all vertebrates (structure varies with species) to regulate water balance and OSMOLARITY. In general, vasopressin is a nonapeptide consisting of a six-amino-acid ring with a cysteine 1 to cysteine 6 disulfide bridge or an octapeptide containing a CYSTINE. All mammals have arginine vasopressin except the pig with a lysine at position 8. Vasopressin, a vasoconstrictor, acts on the KIDNEY COLLECTING DUCTS to increase water reabsorption, increase blood volume and blood pressure.
Different forms of a protein that may be produced from different GENES, or from the same gene by ALTERNATIVE SPLICING.
An ionophorous, polyether antibiotic from Streptomyces chartreusensis. It binds and transports CALCIUM and other divalent cations across membranes and uncouples oxidative phosphorylation while inhibiting ATPase of rat liver mitochondria. The substance is used mostly as a biochemical tool to study the role of divalent cations in various biological systems.
Uridine 5'-(tetrahydrogen triphosphate). A uracil nucleotide containing three phosphate groups esterified to the sugar moiety.
A type of chromogranin which was initially characterized in a rat PHEOCHROMOCYTOMA CELL LINE. It is found in many species including human, rat, mouse, and others. It is an acidic protein with 626 to 657 amino acid residues. In some species, it inhibits secretion of PARATHYROID HORMONE or INSULIN and exerts bacteriolytic effects in others.
The increase in a measurable parameter of a PHYSIOLOGICAL PROCESS, including cellular, microbial, and plant; immunological, cardiovascular, respiratory, reproductive, urinary, digestive, neural, musculoskeletal, ocular, and skin physiological processes; or METABOLIC PROCESS, including enzymatic and other pharmacological processes, by a drug or other chemical.
An enzyme that catalyzes the conversion of phosphatidylinositol (PHOSPHATIDYLINOSITOLS) to phosphatidylinositol 4-phosphate, the first committed step in the biosynthesis of phosphatidylinositol 4,5-bisphosphate.
A subfamily in the family MURIDAE, comprising the hamsters. Four of the more common genera are Cricetus, CRICETULUS; MESOCRICETUS; and PHODOPUS.
A formylated tripeptide originally isolated from bacterial filtrates that is positively chemotactic to polymorphonuclear leucocytes, and causes them to release lysosomal enzymes and become metabolically activated.
A common name used for the genus Cavia. The most common species is Cavia porcellus which is the domesticated guinea pig used for pets and biomedical research.
The fusion of a spermatozoon (SPERMATOZOA) with an OVUM thus resulting in the formation of a ZYGOTE.
The injection of very small amounts of fluid, often with the aid of a microscope and microsyringes.
An amine derived by enzymatic decarboxylation of HISTIDINE. It is a powerful stimulant of gastric secretion, a constrictor of bronchial smooth muscle, a vasodilator, and also a centrally acting neurotransmitter.
Components of a cell produced by various separation techniques which, though they disrupt the delicate anatomy of a cell, preserve the structure and physiology of its functioning constituents for biochemical and ultrastructural analysis. (From Alberts et al., Molecular Biology of the Cell, 2d ed, p163)
Cells grown in vitro from neoplastic tissue. If they can be established as a TUMOR CELL LINE, they can be propagated in cell culture indefinitely.
The commonest and widest ranging species of the clawed "frog" (Xenopus) in Africa. This species is used extensively in research. There is now a significant population in California derived from escaped laboratory animals.
An electrophysiologic technique for studying cells, cell membranes, and occasionally isolated organelles. All patch-clamp methods rely on a very high-resistance seal between a micropipette and a membrane; the seal is usually attained by gentle suction. The four most common variants include on-cell patch, inside-out patch, outside-out patch, and whole-cell clamp. Patch-clamp methods are commonly used to voltage clamp, that is control the voltage across the membrane and measure current flow, but current-clamp methods, in which the current is controlled and the voltage is measured, are also used.
An enzyme formed from PROTHROMBIN that converts FIBRINOGEN to FIBRIN.
A phorbol ester found in CROTON OIL which, in addition to being a potent skin tumor promoter, is also an effective activator of calcium-activated, phospholipid-dependent protein kinase (protein kinase C). Due to its activation of this enzyme, phorbol 12,13-dibutyrate profoundly affects many different biological systems.
An inorganic dye used in microscopy for differential staining and as a diagnostic reagent. In research this compound is used to study changes in cytoplasmic concentrations of calcium. Ruthenium red inhibits calcium transport through membrane channels.
An enzyme that catalyzes the formation of PHOSPHATIDYLINOSITOL and CMP from CDP-DIACYLGLYCEROL and MYOINOSITOL.
Antibodies that inhibit the reaction between ANTIGEN and other antibodies or sensitized T-LYMPHOCYTES (e.g., antibodies of the IMMUNOGLOBULIN G class that compete with IGE antibodies for antigen, thereby blocking an allergic response). Blocking antibodies that bind tumors and prevent destruction of tumor cells by CYTOTOXIC T-LYMPHOCYTES have also been called enhancing antibodies. (Rosen et al., Dictionary of Immunology, 1989)
A characteristic feature of enzyme activity in relation to the kind of substrate on which the enzyme or catalytic molecule reacts.
Thin structures that encapsulate subcellular structures or ORGANELLES in EUKARYOTIC CELLS. They include a variety of membranes associated with the CELL NUCLEUS; the MITOCHONDRIA; the GOLGI APPARATUS; the ENDOPLASMIC RETICULUM; LYSOSOMES; PLASTIDS; and VACUOLES.
Recombinant proteins produced by the GENETIC TRANSLATION of fused genes formed by the combination of NUCLEIC ACID REGULATORY SEQUENCES of one or more genes with the protein coding sequences of one or more genes.
Microscopy of specimens stained with fluorescent dye (usually fluorescein isothiocyanate) or of naturally fluorescent materials, which emit light when exposed to ultraviolet or blue light. Immunofluorescence microscopy utilizes antibodies that are labeled with fluorescent dye.
The degree of similarity between sequences of amino acids. This information is useful for the analyzing genetic relatedness of proteins and species.
A plant genus of the family RHAMNACEAE. Members contain antifungal jujubogenin saponins.
Derivatives of the steroid androstane having two double bonds at any site in any of the rings.
Noninflammatory degenerative disease of the hip joint which usually appears in late middle or old age. It is characterized by growth or maturational disturbances in the femoral neck and head, as well as acetabular dysplasia. A dominant symptom is pain on weight-bearing or motion.
The part of a cell that contains the CYTOSOL and small structures excluding the CELL NUCLEUS; MITOCHONDRIA; and large VACUOLES. (Glick, Glossary of Biochemistry and Molecular Biology, 1990)
A large lobed glandular organ in the abdomen of vertebrates that is responsible for detoxification, metabolism, synthesis and storage of various substances.
The part of CENTRAL NERVOUS SYSTEM that is contained within the skull (CRANIUM). Arising from the NEURAL TUBE, the embryonic brain is comprised of three major parts including PROSENCEPHALON (the forebrain); MESENCEPHALON (the midbrain); and RHOMBENCEPHALON (the hindbrain). The developed brain consists of CEREBRUM; CEREBELLUM; and other structures in the BRAIN STEM.
Any detectable and heritable change in the genetic material that causes a change in the GENOTYPE and which is transmitted to daughter cells and to succeeding generations.
A source of inorganic fluoride which is used topically to prevent dental caries.
The predominant form of mammalian antidiuretic hormone. It is a nonapeptide containing an ARGININE at residue 8 and two disulfide-linked cysteines at residues of 1 and 6. Arg-vasopressin is used to treat DIABETES INSIPIDUS or to improve vasomotor tone and BLOOD PRESSURE.
Inorganic compounds that contain aluminum as an integral part of the molecule.
CELL LINE derived from the ovary of the Chinese hamster, Cricetulus griseus (CRICETULUS). The species is a favorite for cytogenetic studies because of its small chromosome number. The cell line has provided model systems for the study of genetic alterations in cultured mammalian cells.
A class of drugs that act by selective inhibition of calcium influx through cellular membranes.
An octapeptide that is a potent but labile vasoconstrictor. It is produced from angiotensin I after the removal of two amino acids at the C-terminal by ANGIOTENSIN CONVERTING ENZYME. The amino acid in position 5 varies in different species. To block VASOCONSTRICTION and HYPERTENSION effect of angiotensin II, patients are often treated with ACE INHIBITORS or with ANGIOTENSIN II TYPE 1 RECEPTOR BLOCKERS.
The lung volume at which the dependent lung zones cease to ventilate presumably as a result of airway closure.
A family of heterotrimeric GTP-binding protein alpha subunits that activate TYPE C PHOSPHOLIPASES dependent signaling pathways. The Gq-G11 part of the name is also spelled Gq/G11.
The movement of materials (including biochemical substances and drugs) through a biological system at the cellular level. The transport can be across cell membranes and epithelial layers. It also can occur within intracellular compartments and extracellular compartments.
Cell surface proteins that bind PURINES with high affinity and trigger intracellular changes which influence the behavior of cells. The best characterized classes of purinergic receptors in mammals are the P1 receptors, which prefer ADENOSINE, and the P2 receptors, which prefer ATP or ADP.
The movement of ions across energy-transducing cell membranes. Transport can be active, passive or facilitated. Ions may travel by themselves (uniport), or as a group of two or more ions in the same (symport) or opposite (antiport) directions.
The nonstriated involuntary muscle tissue of blood vessels.
Compounds which inhibit or antagonize the biosynthesis or actions of phosphodiesterases.
A lipid phosphatase that acts on phosphatidylinositol-3,4,5-trisphosphate to regulate various SIGNAL TRANSDUCTION PATHWAYS. It modulates CELL GROWTH PROCESSES; CELL MIGRATION; and APOPTOSIS. Mutations in PTEN are associated with COWDEN DISEASE and PROTEUS SYNDROME as well as NEOPLASTIC CELL TRANSFORMATION.
An abnormal anatomical passage between the URINARY BLADDER and the VAGINA.
Proteins which are found in membranes including cellular and intracellular membranes. They consist of two types, peripheral and integral proteins. They include most membrane-associated enzymes, antigenic proteins, transport proteins, and drug, hormone, and lectin receptors.
The study of the generation and behavior of electrical charges in living organisms particularly the nervous system and the effects of electricity on living organisms.
Compounds with three aromatic rings in linear arrangement with an OXYGEN in the center ring.
The species Oryctolagus cuniculus, in the family Leporidae, order LAGOMORPHA. Rabbits are born in burrows, furless, and with eyes and ears closed. In contrast with HARES, rabbits have 22 chromosome pairs.
A network of tubules and sacs in the cytoplasm of SKELETAL MUSCLE FIBERS that assist with muscle contraction and relaxation by releasing and storing calcium ions.
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
A tripeptide that stimulates the release of THYROTROPIN and PROLACTIN. It is synthesized by the neurons in the PARAVENTRICULAR NUCLEUS of the HYPOTHALAMUS. After being released into the pituitary portal circulation, TRH (was called TRF) stimulates the release of TSH and PRL from the ANTERIOR PITUITARY GLAND.
A strain of albino rat developed at the Wistar Institute that has spread widely at other institutions. This has markedly diluted the original strain.
Quinolines substituted in any position by one or more amino groups.
A group of enzymes that are dependent on CYCLIC AMP and catalyze the phosphorylation of SERINE or THREONINE residues on proteins. Included under this category are two cyclic-AMP-dependent protein kinase subtypes, each of which is defined by its subunit composition.
A macrolide antibiotic produced by Streptomyces kitasatoensis. The drug has antimicrobial activity against a wide spectrum of pathogens.
Endogenous substances produced through the activity of intact cells of glands, tissues, or organs.
A partitioning within cells due to the selectively permeable membranes which enclose each of the separate parts, e.g., mitochondria, lysosomes, etc.
A neurotransmitter found at neuromuscular junctions, autonomic ganglia, parasympathetic effector junctions, a subset of sympathetic effector junctions, and at many sites in the central nervous system.
A metallic element that has the atomic number 13, atomic symbol Al, and atomic weight 26.98.
A colorless or slightly yellow crystalline compound obtained from nutgalls. It is used in photography, pharmaceuticals, and as an analytical reagent.
A nucleoside that is composed of ADENINE and D-RIBOSE. Adenosine or adenosine derivatives play many important biological roles in addition to being components of DNA and RNA. Adenosine itself is a neurotransmitter.
Potent activator of the adenylate cyclase system and the biosynthesis of cyclic AMP. From the plant COLEUS FORSKOHLII. Has antihypertensive, positive inotropic, platelet aggregation inhibitory, and smooth muscle relaxant activities; also lowers intraocular pressure and promotes release of hormones from the pituitary gland.
An octapeptide hormone present in the intestine and brain. When secreted from the gastric mucosa, it stimulates the release of bile from the gallbladder and digestive enzymes from the pancreas.
The insertion of recombinant DNA molecules from prokaryotic and/or eukaryotic sources into a replicating vehicle, such as a plasmid or virus vector, and the introduction of the resultant hybrid molecules into recipient cells without altering the viability of those cells.
A protein-serine-threonine kinase that is activated by PHOSPHORYLATION in response to GROWTH FACTORS or INSULIN. It plays a major role in cell metabolism, growth, and survival as a core component of SIGNAL TRANSDUCTION. Three isoforms have been described in mammalian cells.
An oxide of aluminum, occurring in nature as various minerals such as bauxite, corundum, etc. It is used as an adsorbent, desiccating agent, and catalyst, and in the manufacture of dental cements and refractories.
A cyclohexadiene carboxylic acid derived from SHIKIMIC ACID and a precursor for the biosynthesis of UBIQUINONE and the AROMATIC AMINO ACIDS.
A molecule that binds to another molecule, used especially to refer to a small molecule that binds specifically to a larger molecule, e.g., an antigen binding to an antibody, a hormone or neurotransmitter binding to a receptor, or a substrate or allosteric effector binding to an enzyme. Ligands are also molecules that donate or accept a pair of electrons to form a coordinate covalent bond with the central metal atom of a coordination complex. (From Dorland, 27th ed)
Drugs that bind to and activate cholinergic receptors.
A group of cyclic GMP-dependent enzymes that catalyze the phosphorylation of SERINE or THREONINE residues of proteins.
Somewhat flattened, globular echinoderms, having thin, brittle shells of calcareous plates. They are useful models for studying FERTILIZATION and EMBRYO DEVELOPMENT.
Gated, ion-selective glycoproteins that traverse membranes. The stimulus for ION CHANNEL GATING can be due to a variety of stimuli such as LIGANDS, a TRANSMEMBRANE POTENTIAL DIFFERENCE, mechanical deformation or through INTRACELLULAR SIGNALING PEPTIDES AND PROTEINS.
"Inositol 1,4,5-trisphosphate Receptors and Pacemaker Rhythms." Journal of Molecular and Cellular Cardiology 53.3 (2012): 375-81 ... PPY - inhibition of upper GI tract motility GLP-1 - Functions as an "Ileal Brake" to inhibit upper GI tract motility when the ... Current Opinion In Pharmacology [serial online]. December 1, 2013;13(Gastrointestinal * Endocrine and metabolic diseases):928- ... and a normally low one contraction per 30 minutes in the large intestines that increases 3 to 4 times a day due to a phenomenon ...
The enzyme phospholipase C produces diacylglycerol and inositol trisphosphate, which increases calcium ion permeability into ... Yoshida Y, Imai S (June 1997). "Structure and function of inositol 1,4,5-trisphosphate receptor". Japanese Journal of ... Hughes AR, Putney JW (March 1990). "Inositol phosphate formation and its relationship to calcium signaling". Environmental ... Examples of second messenger molecules include cyclic AMP, cyclic GMP, inositol triphosphate, diacylglycerol, and calcium. ...
Inositol 1,4,5-trisphosphate receptor to regulate intracellular Ca2+ signaling; Oct-1 to form the coactivator complex OCA-S, ... 23 (4-5): 603-9. doi:10.1016/s0161-813x(02)00062-1. PMID 12428732. Allen M, Cox C, Belbin O, Ma L, Bisceglio GD, Wilcox SL, ... 44 (1): 189-99. doi:10.1016/j.biocel.2011.10.016. PMID 22062951. Ramos D, Pellín-Carcelén A, Agustí J, Murgui A, Jordá E, ... 33 (1): 203.e25-33. doi:10.1016/j.neurobiolaging.2010.08.002. PMC 3017231. PMID 20864222. Raje CI, Kumar S, Harle A, Nanda JS, ...
Joseph SK, Lin C, Pierson S, Thomas AP, Maranto AR (Oct 1995). "Heteroligomers of type-I and type-III inositol trisphosphate ... Inositol triphosphate Inositol triphosphate receptor GRCh38: Ensembl release 89: ENSG00000150995 - Ensembl, May 2017 GRCm38: ... Inositol 1,4,5-trisphosphate receptor type 1 is a protein that in humans is encoded by the ITPR1 gene. ITPR1 has been shown to ... "Entrez Gene: ITPR1 inositol 1,4,5-triphosphate receptor, type 1". Ando H, Mizutani A, Matsu-ura T, Mikoshiba K (Mar 2003). " ...
There is the formation of a weakly enzyme-bound intermediate, inositol 1,2-cyclic phosphodiester, and release of diacylglycerol ... IP3 is the rate-limiting substrate for the synthesis of inositol polyphosphates, which stimulate multiple protein kinases, ... and inositol 1,4,5-trisphosphate (IP3). Thus PLC has a profound impact on the depletion of PIP2, which acts as a membrane ... The intermediate is then hydrolyzed to inositol 1,4,5-trisphosphate (IP3). Thus the two end products are DAG and IP3. The acid/ ...
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ITPR2 (do inglês, inositol 1,4,5-triphosphate receptor, type 2) é uma proteína que é codificado pelo gene humano ITPR2.[1] A ... Entrez Gene: inositol 1». *↑ Yamamoto-Hino M, Sugiyama T, Hikichi K; et al. (1994). «Cloning and characterization of human type ... Receptor de inositol trifosfato. Referências[editar , editar código-fonte]. *↑ « ... inositol phosphate-mediated signaling. •regulation of insulin secretion. •calcium ion transmembrane transport. •cellular ...
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This leads to the formation of inositol 1,4,5-trisphosphate (IP3) and diacyl glycerol. Due to its hydrophilic character, IP3 ... Sugiyama H, Ito I, Hirono C (1987). "A new type of glutamate receptor linked to inositol phospholipid metabolism". Nature. 325 ... Sladeczek F, Pin JP, Récasens M, Bockaert J, Weiss S (1985). "Glutamate stimulates inositol phosphate formation in striatal ... when it was shown that it could stimulate the formation of inositol phosphates. This finding allowed in 1987 to yield an ...
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... trisphosphate. Soluble inositol-(1,4,5)-trisphosphate triggers calcium to be released from the endoplasmic reticulum. This ... 5 (9): e366. doi:10.1038/mtna.2016.66. PMC 5056990. PMID 27673562. Cao Z, Hao Z, Xin M, Yu L, Wang L, Zhang Y, Zhang X, Guo X ( ... Catenin beta-1, also known as β-catenin, is a protein that in humans is encoded by the CTNNB1 gene. β-catenin is a dual ... 92 (1): 95-105. doi:10.1093/cvr/cvr182. PMID 21693625. Hahn JY, Cho HJ, Bae JW, Yuk HS, Kim KI, Park KW, Koo BK, Chae IH, Shin ...
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Inositol-trisphosphate 3-kinase A is an enzyme that in humans is encoded by the ITPKA gene. ITPKA is one of three inositol- ... Ryu SH, Lee SY, Lee KY, Rhee SG (November 1987). "Catalytic properties of inositol trisphosphate kinase: activation by Ca2+ and ... ITP3K proteins regulate inositol phosphate metabolism by phosphorylation of the second messenger inositol 1,4,5-trisphosphate ... Structurally, ITPKA belongs to the inositol polyphosphate kinase (IPK) family. The activity of the inositol 1,4,5-trisphosphate ...
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The protein encoded by this gene is both a receptor for inositol triphosphate and a calcium channel. Inositol trisphosphate ... Inositol 1,4,5-trisphosphate receptor, type 2, also known as ITPR2, is a protein which in humans is encoded by the ITPR2 gene. ... "Entrez Gene: inositol 1". Yamamoto-Hino M, Sugiyama T, Hikichi K, et al. (1994). "Cloning and characterization of human type 2 ... 2007). "Inositol 1,4,5-trisphosphate receptors are autoantibody target antigens in patients with Sjögren's syndrome and other ...
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November 3 - Michael Berridge and colleagues publish their discovery that inositol trisphosphate acts as a second messenger ... "Release of Ca2+ from a nonmitochondrial intracellular store in pancreatic acinar cells by inositol-1,4,5-trisphosphate". Nature ... September 26 - The Soyuz T-10-1 mission ends in a pad abort at the Baikonur Cosmodrome, when a pad fire occurs at the base of ... 16 (5). Archived from the original on September 28, 2012. Retrieved November 28, 2012. On the net.unix-wizards and net.usoft ...
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... selective desensitization of the inositol trisphosphate/Ca2+ pathway by phosphorylation of the receptor-G protein-coupling ... svi osim 5-HT3) 5-HT1 (A, B, D, E, F) • 5-HT2 (A, B, C) • 5-HT (4, 5A, 6, 7) ... Acetilholin (M1, M2, M3, M4, M5) • Dopamin (D1, D2, D3, D4, D5) • Histamin (H1, H2, H3, H4) • Melatonin (1A, 1B, 1C) • TAAR (1 ... 1,0 1,1 „Entrez Gene: GRM1 glutamate receptor, metabotropic 1".. *^ Stephan D, Bon C, Holzwarth JA, Galvan M, Pruss RM (1996 ...
... produces inositol trisphosphate (IP3). Although inositol trisphosphate diffuses into the cytosol, diacylglycerol remains within ... 0-7167-8724-5. .. [page needed] *^ a b Erion DM, Shulman GI (2010). "Diacylglycerol-mediated insulin resistance". Nature ... 48 (1): 1-8. PMID 3275491.. *^ Berg J, Tymoczko JL, Stryer L (2006). Biochemistry (6th ed.). San Francisco: W. H. Freeman. ISBN ... Diglycerides are a minor component of many seed oils and are normally present at ~1-6%; or in the case of cottonseed oil as ...
Whitman M, Downes CP, Keeler M, Keller T, Cantley L (April 1988). "Type I phosphatidylinositol kinase makes a novel inositol ... trisphosphate. Oriented Peptide Libraries/Scansite. Phosphatidylinositol 5-phosphate. Scientific career. Fields. Biochemistry. ... Traynor-Kaplan AE, Harris AL, Thompson BL, Taylor P, Sklar LA (July 1988). "An inositol tetrakisphosphate-containing ... position on the inositol ring,[8] and that this phosphatidylinositol-3-kinase (PI-3-kinase) is activated by growth factors to ...
inositol 1,4,5 trisphosphate binding. • ion channel activity. • protein binding. • actin binding. • calcium channel activity. • ... 308 (5729): 1801-4. doi:10.1126/science.1106215. PMID 15879175.. *^ Hisatsune C, Kuroda Y, Nakamura K, Inoue T, Nakamura T, ... 57 (4): 427-50. doi:10.1124/pr.57.4.6. PMID 16382100.. *. Schlöndorff JS, Pollak MR (December 2006). "TRPC6 in glomerular ... It has been associated with depression and anxiety (see below), as well as with focal segmental glomerulosclerosis (FSGS).[5] ...
... (InsP3R) is a membrane glycoprotein complex acting as a Ca2+ channel activated by inositol ... Inositol+Trisphosphate+Receptor at the US National Library of Medicine Medical Subject Headings (MeSH) ... and characterization of an inositol trisphosphate receptor" (PDF). The Journal of Biological Chemistry. 263 (3): 1530-4. PMID ... and soon thereafter it was confirmed that the protein p400 was in fact the inositol trisphosphate receptor.[6] ...
Guan X, Wenk MR (May 2008). "Biochemistry of inositol lipids". 》Frontiers in Bioscience》 13 (13): 3239-51. doi:10.2741/2923. ... Berridge MJ, Irvine RF (September 1989). "Inositol phosphates and cell signalling". 》Nature》 341 (6239): 197-205. doi:10.1038/ ... Klein C, Malviya AN (January 2008). "Mechanism of nuclear calcium signaling by inositol 1,4,5-trisphosphate produced in the ... 일반적으로 4~24개의 탄소로 구성되는 탄소 사슬[21]은 포화되거나 불포화될 수 있으며, 산소, 할로젠, 질소, 황이 포함된 작용기가 부착될 수도 있다. 지방산이 이중 결합을 포함하고 있으면 시스 또는 트랜스 기하 이성질화 ...
positive regulation of inositol trisphosphate biosynthetic process. • positive regulation of cardiac vascular smooth muscle ... doi:10.1007/s12672-014-0174-1. PMC 4091989. PMID 24718936.. *^ a b c Lappano R, Pisano A, Maggiolini M (2014). "GPER Function ... trisphosphate in the nucleus.[8] This protein therefore plays a role in the rapid nongenomic signaling events widely observed ... 74: 1-11. doi:10.1146/annurev-physiol-020911-153327. PMID 21888507.. *^ a b Vrtačnik P, Ostanek B, Mencej-Bedrač S, Marc J ( ...
"Inositol 1,4,5-trisphosphate Receptors and Pacemaker Rhythms." Journal of Molecular and Cellular Cardiology 53.3 (2012): 375-81 ... The frequency in the stomach is 3 per minute, while the duodenum is 11 to 12 per minute and the ileum is 9 per minute.[1] The ... GLP-1 - Functions as an "Ileal Brake" to inhibit upper GI tract motility when the distal gut is exposed to unabsorbed nutrients ... This electrical rhythm is spread through gap junctions in the smooth muscle of the GI tract.[1] These pacemaker cells, also ...
... trisphosphate secondary messengers; Phospholipase C (PLC) which when activated is responsible for forming Inositol ... classification[5]. G protein linkage[2]. pathways[2] Prostaglandin DP1 receptor. DP1. PGD2,,PGE2,PGF2α,PGI2=TXA2[6]. relaxant. ... 79 (4): 1193-226. doi:10.1152/physrev.1999.79.4.1193. PMID 10508233.. *^ a b "Prostanoid receptors - G protein-coupled ... EP1. PGE2,PGF2α=PGI2,PGD2=TXA2[8]. contractile. Gq alpha subunit. stimulates PLC, IP3, PKC, ERK, p38 Mpk, and CREB ...
... selective desensitization of the inositol trisphosphate/Ca2+ pathway by phosphorylation of the receptor-G protein-coupling ... Acetilholin (M1, M2, M3, M4, M5) • Dopamin (D1, D2, D3, D4, D5) • Histamin (H1, H2, H3, H4) • Melatonin (1A, 1B, 1C) • TAAR (1 ... CysLT (1, 2) • LTB4 (1, 2) • FPRL1 • OXE • Prostaglandin (DP (1, 2), EP (1, 2, 3, 4), FP) • Prostaciklin • Tromboksan ... B/W (1, 2) • FF (1, 2) • S • Y (1, 2, 4, 5) • Neuromedin (B, U (1, 2)) • Neurotenzin (1, 2) ...
1b55: PH DOMAIN FROM BRUTON'S TYROSINE KINASE IN COMPLEX WITH INOSITOL 1,3,4,5-TETRAKISPHOSPHATE ... trisphosphate (PIP3). PIP3 binding induces Btk to phosphorylate phospholipase C, which in turn hydrolyzes PIP2, a ... Phase 1: *ONO-4059 for Non-Hodgkin's Lymphoma and/or CLL.[9] Renamed GS-4059 and now in trial NCT02457598.[10] ... 4 (4): 294-7. PMID 12001708.. *. Niiro H, Clark EA (2003). "Branches of the B cell antigen receptor pathway are directed by ...
There are seven subfamilies of Kir channels, denoted as Kir1 - Kir7.[1] Each subfamily has multiple members (i.e. Kir2.1, Kir ... Figure 1. Whole-cell current recordings of Kir2 inwardly-rectifying potassium channels expressed in an HEK293 cell. (This is a ... To date, seven subfamilies have been identified in various mammalian cell types,[1] plants,[2] and bacteria.[3] They are the ... "1.A.2 Inward Rectifier K Channel (IRK-C) Family". TCDB. Retrieved 2016-04-09.. ...
98 (2): 172-5. doi:10.1007/s004390050183. PMID 8698335.. *^ a b c d e f g h i j "GJB1 gene". Genetics Home Reference. US ... doi:10.1007/s10048-010-0247-4. PMC 4222676 . PMID 20532933.. *^ a b c d e f g h i Bergoffen J, Scherer SS, Wang S, Scott MO, ... 126 (Pt 1): 134-51. doi:10.1093/brain/awg012. PMID 12477701.. *. Sato H, Hagiwara H, Ohde Y, Senba H, Virgona N, Yano T (March ... Gap junction beta-1 protein (GJB1), also known as connexin 32 (Cx32) is a transmembrane protein that in humans is encoded by ...
... inositol 1,4,5-trisphosphate (IP3) and diacylglycerol (DAG). IP3 binds with the IP3 receptor in the membrane of the smooth ... trisphosphate (IP3) and diacylglycerol (DAG). IP3 acts on IP3 receptors found in the membrane of the endoplasmic reticulum (ER ... 4 (6): 905-907. doi:10.1021/cn400107y. PMC 3689196. PMID 24024784.. *^ a b c Bjarnadóttir TK, Gloriam DE, Hellstrand SH, ... 85 (4): 1159-204. doi:10.1152/physrev.00003.2005. PMID 16183910.. *^ a b Hauser AS, Chavali S, Masuho I, Jahn LJ, Martemyanov ...
Pyridine nucleotide metabolites stimulate calcium release from sea urchin egg microsomes desensitized to inositol trisphosphate ... ISBN 1-40207-281-3. Història. *Cornish-Bowden, Athel. New Beer in an Old Bottle. Eduard Buchner and the Growth of Biochemical ... 2,0 2,1 2,2 2,3 2,4 2,5 Belenky P; Bogan KL, Brenner C «NAD+ metabolism in health and disease» (PDF). Trends Biochem. Sci., 32 ... 282-5. DOI: 10.1016/j.ab.2006.02.017. PMID: 16574057. *↑ 12,0 12,1 Yang H, Yang T, Baur JA, Perez E, Matsui T, Carmona JJ, ...
... inositol-1,4,5-trisphosphate (IP3); PI3K also acts on PIP2, phosphorylating it to produce phosphatidlyinositol-3,4,5- ... trisphosphate (PIP3). DAG binds and activates some PKCs. Most important in T cells is PKCθ, critical for activating the ... 5: 450. doi:10.3389/fimmu.2014.00450. PMC 4189401. PMID 25339949.. *^ a b c Howson LJ, Salio M, Cerundolo V (2015-06-16). "MR1- ... 15 (4): R183. doi:10.1186/cc10332. PMC 3387626. PMID 21798063.. *^ a b Boomer JS, To K, Chang KC, Takasu O, Osborne DF, Walton ...
"Pyridine nucleotide metabolites stimulate calcium release from sea urchin egg microsomes desensitized to inositol trisphosphate ... 3,0 3,1 3,2 3,3 3,4 3,5 Belenky P; Bogan KL, Brenner C (2007). "NAD+ metabolism in health and disease" (PDF). Trends Biochem. ... Biol. 13 (5): 408-13. PMID 16648861. doi:10.1038/nsmb1089.. *↑ 73,0 73,1 Pankiewicz KW, Patterson SE, Black PL; et al. (2004 ... J. 115 (4): 609-19. PMC 1185185. PMID 4391039.. *↑ Katoh A, Uenohara K, Akita M, Hashimoto T (2006). "Early steps in the ...
Guan X, Wenk MR (May 2008). "Biochemistry of inositol lipids". Frontiers in Bioscience. 13 (13): 3239-51. doi:10.2741/2923. ... Berridge MJ, Irvine RF (September 1989). "Inositol phosphates and cell signalling". Nature. 341 (6239): 197-205. doi:10.1038/ ... Klein C, Malviya AN (January 2008). "Mechanism of nuclear calcium signaling by inositol 1,4,5-trisphosphate produced in the ... 978-1-4051-3066-0. .. *^ a b c Kuzuyama T, Seto H (April 2003). "Diversity of the biosynthesis of the isoprene units". Natural ...
Although inositol trisphosphate, (IP3), diffuses into the cytosol, diacylglycerol (DAG) remains within the plasma membrane, due ... Inositol trisphosphate (IP3) functions as an intracellular second messenger, which initiates the intracellular release of ... These phospholipids can be cleaved into diacylglycerol (DAG) and inositol trisphosphate (IP3) through hydrolysis of the ... For example, 1 g of glycogen can bind approximately 2 g of water, which translates to 1.33 kcal/g (4 kcal/3 g). This means that ...
... inositol trisphosphate, V-ATPase and ARNO (a guanine nucleotide exchange factor of ARF6). These associations are thought to be ... 1,3-bisphosphoglycerate + NAD(P)H + H+ ⇌ G3P + Pi + NAD(P)+. Triose-phosphate isomerase maintains DHAP and G3P in near ... 2(PEP + NADH + H+ + ATP + H2O) ⇌ fructose 1,6-bisphosphate + 2(NAD+ + ADP + Pi). Aldolase is also used in the part of the ... Two histidine residues in the first half of the sequence of these homologs have been shown to be involved in binding zinc.[1] ...
Wekesa KS, Anholt RR; Anholt (August 1997). "Pheromone regulated production of inositol-(1, 4, 5)-trisphosphate in the ... As neuronas do OVN expresan receptores de tres familias, chamados V1R,[2] V2R,[3][4][5] e FPR.[6][7] Os receptores son ... 5 December 2007. Consultado o 26 September 2009.. *↑ Chamero P, Marton TF, Logan DW, et al. (December 2007). "Identification of ... 27,0 27,1 Simon, Verne A. (2010). Adaptations in the Animal Kingdom. Xlibris Corp. p. 31. ISBN 1450033644.. ...
This change activates the G-protein, gustducin, which in turn activates phospholipase C to generate inositol trisphosphate (IP3 ... 978-0-13-286261-5. .. *^ Dermer, OC (1947). "The Science of Taste". Proceedings of the Oklahoma Academy of Science. 27: 15-18. ... 20 (5): 573-584. doi:10.1093/chemse/20.5.573. PMID 8564432.. *^ Biello, David (August 16, 2007). "Strange but True: Cats Cannot ... 283 (1): 236-242. doi:10.1006/bbrc.2001.4760. PMID 11322794.. *. Max M, Shanker YG, Huang LQ, Rong M, Liu Z, Campagne F, ...
... and a reduction of ankyrin-B in neonatal cardiomyocytes reduces the half-life of the inositol trisphosphate receptor by 3-fold ... loops within the ankyrin repeat domain of ankyrin-B are required for the interaction with the inositol trisphosphate receptor, ... domain of ankyrin-B determines both the subcellular localization as well as activity in restoring normal inositol trisphosphate ... Mohler, PJ; Davis, JQ; Davis, LH; Hoffman, JA; Michaely, P; Bennett, V (26 March 2004). "Inositol 1,4,5-trisphosphate receptor ...
Inositol triphosphate receptor. *Ion channels. *NMDA receptors. References[edit]. *^ a b Catterall WA, Perez-Reyes E, Snutch TP ... α1 subunit (gene name). Associated subunits. Most often found in L-type calcium channel ("Long-Lasting" AKA "DHP Receptor"). ... 1,4-dihydropyridine sensitivity (DHP). ω-conotoxin sensitivity (ω-CTX). ω-agatoxin sensitivity (ω-AGA) ... 27 (1-4): 201-6. doi:10.1152/advan.00025.2003. PMID 14627618.. *^ Alberts B, Johnson A, Lewis J, Raff M, Roberts K, Walter P ( ...
Biochemistry of inositol lipids. Frontiers in Bioscience. 2008, 13 (13): 3239-51. PMID 18508430. doi:10.2741/2923.. ... 脂類(英語:Lipid),又稱脂質,这是一类不溶于水而易溶于脂肪溶剂(醇、醚、氯仿、苯)等非极性有机溶剂,由脂肪酸与醇作用脱水缩合生成的酯及其衍生物统称为脂类,其中包括脂肪、蠟、类固醇、脂溶性維生素(如維生素A,
... enterotoxin NSP4 mobilizes intracellular calcium in human intestinal cells by stimulating phospholipase C-mediated inositol 1,4 ... Virology, 1988, nro 139 (1), s. 89-99. (englanniksi) *↑ a b c d Bishop, R. F.: Natural history of human rotavirus infection. ... vsk, nro 52 (4), s. 442-445. (englanniksi) *↑ a b Woode, G. N. ym.: Morphological and antigenic relationships between viruses ( ... 1, s. 12-18. (englanniksi) *↑ Cook S. M. ym.: Global seasonality of rotavirus infections. Bulletin of the World Health ...
"In recognition of his discovery that inositol 1,4,5-trisphosphate functions as a second messenger to mobilize calcium.". ... وذلك في نطاق دول الكومنولث[1].. ابتدع القلادة الملك جورج الرابع ومُنحت للمرة الأولى سنة 1826. وكانت تُمنح في البداية قلادتان ... "ب"[1]. وقد مُنحت القلادة 405 مرات منذ ابتداعها سنة 1826. ...
Structural studies of inositol 1,4,5-trisphosphate receptor: coupling ligand binding to channel gating.. J. Biol. Chem. 285 ... GO:0070679 inositol 1,4,5 trisphosphate binding GO:0005220 inositol 1,4,5-trisphosphate-sensitive calcium-release channel ... The inositol 1,4,5-trisphosphate (IP3)3 receptor (IP3R) are tetrameric intracellular Ca2+ release channels on the endoplasmic ... Tyr-167/Trp-168 in type 1/3 inositol 1,4,5-trisphosphate receptor mediates functional coupling between ligand binding and ...
Structural Analyses of Inositol 1,4,5-Trisphosphate 3-Kinase Message Subject. (Your Name) has forwarded a page to you from ... B. González, M. J. Schell, A. J. Letcher, D. B. Veprintsev, R. F. Irvine, R. L. Williams, Structure of a human inositol 1,4,5- ... G. J. Miller, J. H. Hurley, Crystal structure of the catalytic core of inositol 1,4,5-trisphosphate 3-kinase. Mol. Cell 15, 703 ... to produce inositol 1,4,5,6-tetrakisphosphate. González et al. crystallized residues 187 to 461 of the human IP3-3K A isoform, ...
Identification of a family of calcium sensors as protein ligands of inositol trisphosphate receptor Ca(2+) release channels. ... Receptor for inositol 1,4,5-trisphosphate, a second messenger that mediates the release of intracellular calcium.. Specific ... calcium ion binding / calcium-release channel activity / inositol 1,3,4,5 tetrakisphosphate binding / inositol 1,4,5 ... trisphosphate binding / inositol 1,4,5-trisphosphate-sensitive calcium-release channel activity / inositol hexakisphosphate ...
Preparation of Quality Inositol Pyrophosphates, Characterization of G Protein-coupled Receptors by a Fluorescence-based ... Video articles in JoVE about inositol 1 4 5 trisphosphate include Imaging Local Ca2+ Signals in Cultured Mammalian Cells, ... 5-bisphosphate in the Plasma Membrane with Novel Fluorescent Probes, Measuring Physiological Responses of Drosophila Sensory ... Preparation of Quality Inositol Pyrophosphates. Omar Loss1, Cristina Azevedo1, Zsolt Szijgyarto1, Daniel Bosch1, Adolfo Saiardi ...
d-myo-inositol 1,4,5-trisphosphate (Ins(1,4,5)P3, or IP3) is a ubiquitous second messenger that regulates cytosolic Ca2+ ... Cell membrane permeable esters of D-myo-inositol 1,4,5-trisphosphate Cell Calcium. 2007 Sep;42(3):291-301. doi: 10.1016/j.ceca. ... d-myo-inositol 1,4,5-trisphosphate (Ins(1,4,5)P3, or IP3) is a ubiquitous second messenger that regulates cytosolic Ca2+ ... from myo-inositol in 9 steps. Ci-IP3/PM is a homologue of cm-IP3/PM, a caged and cell permeable IP3 ester developed earlier. In ...
Hajnoczky, G. and Thomas, A. P. (1994). The inositol trisphosphate calcium channel is inactivated by inositol trisphosphate. ... Thus, an inositol trisphosphate receptor can act as a RAS-independent, tissue-specific positive effector of LET-23. An inositol ... of the inositol trisphosphate receptor (Sayers, 1997). Inositol 1,4,5-trisphosphate receptor (InsP3R) is an inositol InsP3- ... Clandinin, T. R., DeModena, J. A. and Sternberg, P. W. (1998). Inositol trisphosphate mediates a RAS-independent response to ...
... Cell. 1995 Nov 3;83(3 ... A M Cameron 1 , J P Steiner, A J Roskams, S M Ali, G V Ronnett, S H Snyder ... 1 Department of Neuroscience, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA. ...
Receptor for inositol 1,4,5-trisphosphate, a second messenger that mediates the release of intracellular calcium. This release ... Inositol 1,4,5-trisphosphate receptor type 2Add BLAST. 2701. Amino acid modifications. Feature key. Position(s). Description ... Inositol 1,4,5-trisphosphate bindingBy similarity. 5. ,p>This subsection of the Family and Domains section describes a region ... Inositol 1,4,5-trisphosphate bindingBy similarity. 4. ,p>This subsection of the Family and Domains section describes a region ...
Inositol 1,4,5-trisphosphate receptor type 2Imported. ,p>Information which has been imported from another database using ... tr,B2KF89,B2KF89_MOUSE Inositol 1,4,5-trisphosphate receptor type 2 (Fragment) OS=Mus musculus GN=Itpr2 PE=1 SV=1 ... 1. Imported. ,p>Information which has been imported from another database using automatic procedures.,/p> ,p>,a href="/manual/ ... Annotation score:1 out of 5. ,p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB ...
Sipma H1, Deelman L, Smedt HD, Missiaen L, Parys JB, Vanlingen S, Henning RH, Casteels R. ... 1. Laboratorium voor Fysiologie, Campus Gasthuisberg O/N, KU Leuven, Belgium.. Abstract. Prolonged stimulation of rat A7r5 ... Agonist-induced down-regulation of type 1 and type 3 inositol 1,4,5-trisphosphate receptors in A7r5 and DDT1 MF-2 smooth muscle ... InsP3R-1) and of the type 3 InsP3 receptor (InsP3R-3). InsP3R down-regulation was a cell- and agonist-specific process, since ...
In mouse eggs, these oscillations cease after a variable period of time and this is accompanied by a decrease in inositol 1,4,5 ... Down-regulation of the inositol 1,4,5-trisphosphate receptor in mouse eggs following fertilization or parthenogenetic ... trisphosphate receptor (IP3R) responsiveness and down-regulation of the IP3R type 1 (IP3R-1). To investigate the signaling ... Jellerette T1, He CL, Wu H, Parys JB, Fissore RA.. Author information. 1. Department of Veterinary and Animal Sciences, ...
Han-Chen Ho and Susan S. Suarez "An Inositol 1,4,5-Trisphosphate Receptor-Gated Intracellular Ca2 Store Is Involved in ... An Inositol 1,4,5-Trisphosphate Receptor-Gated Intracellular Ca2 Store Is Involved in Regulating Sperm Hyperactivated Motility ... Han-Chen Ho, Susan S. Suarez "An Inositol 1,4,5-Trisphosphate Receptor-Gated Intracellular Ca2 Store Is Involved in Regulating ... Indirect immunofluorescence labeling showed type I inositol 1,4,5-trisphosphate receptors (IP3R) in the acrosome and neck ...
Inositol 1,4,5-trisphosphate (IP3) is generally viewed as a global messenger that increases cytosolic calcium ion (Ca2+) ... Control of Neuronal Growth Cone Navigation by Asymmetric Inositol 1,4,5-Trisphosphate Signals ... Control of Neuronal Growth Cone Navigation by Asymmetric Inositol 1,4,5-Trisphosphate Signals ... Control of Neuronal Growth Cone Navigation by Asymmetric Inositol 1,4,5-Trisphosphate Signals ...
The inositol 1,4,5-trisphosphate (IP3) receptor (IP3R) acts as a Ca2+ release channel on internal Ca2+ stores. Type 1 IP3R (IP3 ... Regulation of Nerve Growth Mediated by Inositol 1,4,5-Trisphosphate Receptors in Growth Cones ... Regulation of Nerve Growth Mediated by Inositol 1,4,5-Trisphosphate Receptors in Growth Cones ... Regulation of Nerve Growth Mediated by Inositol 1,4,5-Trisphosphate Receptors in Growth Cones ...
Khodakhah K, Ogden D (1995) Fast activation and inactivation of inositol trisphosphate-evoked Ca2+ release in rat cerebellar ... Inositol 1,4,5-Trisphosphate-Dependent Ca2+ Threshold Dynamics Detect Spike Timing in Cerebellar Purkinje Cells. Tomokazu Doi, ... Irvine RF, Schell MJ (2001) Back in the water: the return of the inositol phosphates. Nat Rev Mol Cell Biol 2: 327-338. ... Adkins CE, Taylor CW (1999) Lateral inhibition of inositol 1,4,5-trisphosphate receptors by cytosolic Ca2+. Curr Biol 9: 1115- ...
Inositol 1,4,5-trisphosphate receptors (IP3Rs) constitute a family of Ca2+ channels that is responsible for Ca2+ mobilization ... 1995) Inositol 1, 4, 5-trisphosphate receptor-mediated Ca2+ signaling in the brain. J Neurochem 64:953-960. ... 2004) Distinct roles of inositol 1,4,5-trisphosphate receptor types 1 and 3 in Ca2+ signaling. J Biol Chem 279:11967-11975. ... Inositol 1,4,5-Trisphosphate Receptor Type 1 in Granule Cells, Not in Purkinje Cells, Regulates the Dendritic Morphology of ...
MRV integration site 1; Mrvi1; Murine retrovirus integration site 1 homolog Inositol... ... Inositol 1,4,5-trisphosphate (IP3) receptor-associated cyclic GMP (cGMP) kinase substrate (IRAG) is a 125-kD type 2 integral ... 1999). IRAG forms a complex with the inositol 1,4,5-trisphosphate receptor (IP3R) and cGMP kinase I-β (cGKIβ) and is involved ... Grillo M.A., Koulen P. (2018) Inositol 1,4,5-Trisphosphate-Associated cGMP Kinase Substrate. In: Choi S. (eds) Encyclopedia of ...
Expression of the cDNA in COS cells resulted in an approximately 150-fold increase in inositol 1,4,5-trisphosphate 3-kinase ... A complementary DNA (cDNA) clone that encodes inositol 1,4,5-trisphosphate 3-kinase was isolated from a rat brain cDNA ... Molecular cloning and expression of a complementary DNA for inositol 1,4,5-trisphosphate 3-kinase ... Molecular cloning and expression of a complementary DNA for inositol 1,4,5-trisphosphate 3-kinase ...
Emerging roles of inositol 1,4,5-trisphosphate signaling in cardiac myocytes.. Journal of Molecular and Cellular Cardiology, 45 ... Inositol 1,4,5-trisphosphate (IP3) is a ubiquitous intracellular messenger regulating diverse functions in almost all mammalian ... Emerging roles of inositol 1,4,5-trisphosphate signaling in cardiac myocytes ... inositol 1,4,5-trisphosphate; cardiac myocyte; calcium; inotropy; arrhythmias; nucleus; hypertrophy ...
Inositol 1,4,5-trisphosphate receptors selectively localized to the acrosomes of mammalian sperm.. L D Walensky, S H Snyder ... We have characterized inositol 1,4,5-trisphosphate (IP3)-gated calcium channels and upstream components of the phosphoinositide ... Inositol 1,4,5-trisphosphate receptors selectively localized to the acrosomes of mammalian sperm. ... The presence of G alpha q/11, PLC beta 1 and a functional IP3R in the anterior acrosomal region of mammalian sperm, as well as ...
... on inositol 1,4,5-trisphosphate-induced calcium release from porcine cerebellar microsomes: THA+ can induce calcium release ... In this study we show that the potassium-channel blocker tetrahexyl ammonium chloride (THA+) is able to inhibit inositol 1,4,5- ... The effects of tetrahexyl ammonium cations (THA+) on inositol 1,4,5-trisphosphate-induced calcium release from porcine ... The effects of tetrahexyl ammonium cations (THA+) on inositol 1,4,5-trisphosphate-induced calcium release from porcine ...
The effects of insulin in ob/ob cells could be reproduced by application of a membrane-permeant inositol trisphosphate (IP3) ... Insulin and Inositol 1,4,5-Trisphosphate Trigger Abnormal Cytosolic Ca2+ Transients and Reveal Mitochondrial Ca2+ Handling ... Mackenzie L, Bootman MD, Laine M, Berridge MJ, Thuring J, Holmes A, Li WH, Lipp P: The role of inositol 1,4,5-trisphosphate ... Jaconi M, Bony C, Richards SM, Terzic A, Arnaudeau S, Vassort G, Puceat M: Inositol 1,4,5-trisphosphate directs Ca2+ flow ...
Berridge MJ (2016) The inositol trisphosphate/calcium signaling pathway in health and disease. Physiol Rev 96:1261-1296CrossRef ... Narayanan D, Adebiyi A, Jaggar JH (2012) Inositol trisphosphate receptors in smooth muscle cells. Am J Physiol Heart Circ ... Type 2 inositol 1,4,5-trisphosphate receptor inhibits the progression of pulmonary arterial hypertension via calcium signaling ... In this study, we found the specific expression of type 2 inositol 1,4,5-trisphosphate receptor (IP3R2), which is an ...
... inositol tetrakisphosphate or to [3H]inositol 1,3,4-trisphosphate, which suggests that the 1,3,4 isomer does not result from ... Metabolism of inositol 1,4,5-trisphosphate in guinea-pig hepatocytes. K A Tennes, J S McKinney, J W Putney Jr ... Metabolism of inositol 1,4,5-trisphosphate in guinea-pig hepatocytes Message Subject (Your Name) has forwarded a page to you ... The half-time of hydrolysis of inositol 1,3,4,5-tetrakis[32P]phosphate of approx. 1 min was increased to 3 min by 2,3- ...
Taken together, our results suggest that in myeloid cells (i) SERCA-type Ca2+-ATPases function as Ca2+ pumps of Ins(1,4,5)P3- ... In this study, we have analysed the relationship between Ca2+ pumps and Ins(1,4,5)P3-sensitive Ca2+ channels in myeloid cells. ... Organization of Ca2+ stores in myeloid cells: association of SERCA2b and the type-1 inositol-1,4,5-trisphosphate receptor ... They also decreased, in parallel, ATP-dependent Ca2+ accumulation and the amount of Ins(1,4,5)P3-releasable Ca2+. ...
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Transformation of Rat-1 fibroblasts with the v-src oncogene induces inositol 1,4,5-trisphosphate 3-kinase expression Pamela J ... Transformation of Rat-1 fibroblasts with the v-src oncogene leads to a 6- to 8-fold enhancement of the activity of the Ins(1,4, ... The mRNA and protein levels for two rat isoforms of Ins(1,4,5)P3 3-kinase were determined in the v-src-transformed cell line. ... Moreover, stable expression of either form of Ins(1,4,5)P3 3-kinase in the B31 v-src-transformed Rat-1 cell line did not result ...
263, 15297-15303] paradoxically high levels of inositol 1,4,5-trisphosphate [(1,4,5)IP3] in resting and s ... Inositol / metabolism. Inositol 1,4,5-Trisphosphate / metabolism, pharmacology, physiology*. Kinetics. Substance P / ... Relationship between the calcium-mobilizing action of inositol 1,4,5-trisphosphate in permeable AR4-2J cells and the estimated ... 263, 15297-15303] paradoxically high levels of inositol 1,4,5-trisphosphate [(1,4,5)IP3] in resting and substance-P-stimulated ...
... activates both adenylyl cyclase and the phosphoinositide cascade and that the changes in the levels of cAMP and inositol-1,4,5- ... trisphosphate (IP3) produced are species specific. In the present study, we examined the mechanism of the ET-1 effects in cat ... 2) ET-1 dose-dependently evoked PGe2 release (EC50 = 1.8 nM), IP3 production (EC50 = 4.5 nM) and contraction (EC50 = 5 nM) and ... 4) PGE2 (1 microM), increased IP3 production by 55% and induced muscle contraction in a dose-dependent manner (EC50 = 40 nM). ...
  • Inositol 1,4,5-trisphosphate receptors (IP3R) are Ca2+ channels on the neuronal endoplasmic reticulum (ER) membrane. (sdbonline.org)
  • Agonist-induced down-regulation of type 1 and type 3 inositol 1,4,5-trisphosphate receptors in A7r5 and DDT1 MF-2 smooth muscle cells. (nih.gov)
  • Indirect immunofluorescence labeling showed type I inositol 1,4,5-trisphosphate receptors (IP 3 R) in the acrosome and neck region, but no ryanodine receptors (RyR) were found using anti-RyR antibodies or BODIPY FL-X ryanodine. (bioone.org)
  • It is generated by membrane receptors that couple to phospholipase C (PLC), an enzyme which liberates IP 3 from phosphatidylinositol 4,5-bisphosphate (PIP 2 ). (open.ac.uk)
  • Inositol 1,4,5-trisphosphate receptors selectively localized to the acrosomes of mammalian sperm. (rupress.org)
  • The density of (1,4,5)IP3 receptors in AR4-2J cells was not sufficiently large to impose non-linearity in the relationship between (1,4,5)IP3 concentration and (1,4,5)IP3-induced Ca2+ release. (biomedsearch.com)
  • Thus the apparent high concentrations of (1,4,5)IP3 in resting and stimulated AR4-2J cells are not indicative of atypically low sensitivity or high concentration of (1,4,5)IP3 receptors, nor is there evidence for compartmentalization of (1,4,5)IP3 outside of the cytoplasm in these cells. (biomedsearch.com)
  • It is possible that soluble factors in the cytoplasm of AR4-2J cells regulate the free concentration of (1,4,5)IP3 or the sensitivity of receptors to (1,4,5)IP3. (biomedsearch.com)
  • Ins(1,4,5)P3 is the intracellular messenger that in many cells mediates the effects of Ca2(+)-mobilizing receptors on intracellular Ca2+ stores. (biochemj.org)
  • Although ventricular cardiomyocytes express inositol 1,4,5-trisphosphate [Ins(1,4,5)P-3] receptors, it is unclear how these Ca2+ channels contribute to the effects of Gq-coupled agonists. (ucl.ac.uk)
  • The endothelin-1-mediated spontaneous Ca2+ transients were abolished by application of 2-aminoethoxydiphenyl borate (2-APB), an antagonist of Ins(1,4,5)P-3 receptors. (ucl.ac.uk)
  • We propose that Ins(1,4,5)P-3 receptors support the development of both inotropy and spontaneous pro-arrhythmic Ca2+ signals in ventricular myocytes stimulated with a Gq-coupled agonist. (ucl.ac.uk)
  • Stimulation of basal secretagogue receptors as well as uncaging InsP 3 in various regions of the cell also confirm that Ca 2+ signals initiate in apical regions of the pancreatic acinar cell ( 5 , 20 , 31 , 58 , 65 ). (pancreapedia.org)
  • Expression of inositol 1,4,5-trisphosphate receptors in rat adrenocortical zones. (scienceexchange.com)
  • Inositol 1,4,5-Trisphosphate Receptors in Hypertension. (edu.qa)
  • Changes in free intracellular calcium levels ([Ca] ) are mediated either by the influx of Ca from the extracellular space or release of Ca from intracellular stores, mainly the sarcoplasmic reticulum (SR). The influx of extracellular Ca occurs primarily through voltage-gated Ca channels (VGCCs), store-operated Ca channels (SOC), and Ca release-activated channels (CRAC), whereas SR-Ca release occurs through inositol trisphosphate receptor (IPR) and ryanodine receptors (RyRs). (edu.qa)
  • Tec kinases are nonreceptor tyrosine kinases that modulate signals downstream of many hemopoietic surface receptors, including AgRs, cytokine receptors, integrins, and G protein-coupled receptors ( 4 ). (jimmunol.org)
  • Knockout studies in mice suggest that type 2 and type 3 inositol 1,4,5-trisphosphate receptors play a key role in exocrine secretion underlying energy metabolism and growth. (sigmaaldrich.com)
  • Inositol 1,4,5-trisphosphate (IP3) receptors are a form of ligand-gated ion channels that are activated by cytosolic Ca2+ and IP3. (genecards.org)
  • The function of inositol 1,4,5-trisphosphate receptors (IP3Rs) in the human heart is largely unknown and may have important implications in the modulation of myocardial performance and rhythm disturbances. (ahajournals.org)
  • Distribution and functional role of inositol 1,4,5-trisphosphate receptors in mouse sinoatrial node. (ox.ac.uk)
  • RATIONALE: Inositol 1,4,5-trisphosphate receptors (IP(3)Rs) have been implicated in the generation of arrhythmias and cardiac muscle nuclear signaling. (ox.ac.uk)
  • The ubiquitous inositol 1,4,5‐trisphosphate receptor (InsP 3 R) intracellular Ca 2+ release channel is engaged by thousands of plasma membrane receptors to generate Ca 2+ signals in all cells. (embopress.org)
  • The authors hypothesized that isoflurane activates inositol 1,4,5-trisphosphate (IP3) receptors on the endoplasmic reticulum (ER) membrane, causing excessive calcium release, triggering apoptosis. (asahq.org)
  • The authors used the chicken B lymphocyte with a total knock-out of IP3 receptors, PC12 cells with elevated IP3 receptor activity (transfected with L286V presenilin 1), striatal cells with a knock-in of Q111 Huntingtin, and each cell line's corresponding wild-type controls. (asahq.org)
  • Ryanodine receptors (RyRs) and inositol 1,4,5-trisphosphate receptors (IP3Rs) are users of a family of tetrameric intracellular Ca2+-launch channels (CRCs). (neuroart2006.com)
  • Ryanodine receptors (RyRs) and inositol 1,4,5-trisphosphate receptors (IP3Rs) are large tetrameric intracellular Ca2+-launch channels (CRCs) located in the endo/sarcoplasmic reticulum (ER/SR) of cells. (neuroart2006.com)
  • Receptors Channels 1 (1993) 11-24. (biomedcentral.com)
  • The inositol 1,4,5-trisphosphate receptors (IP3Rs) as ligandgated Ca 2+ channels are key modulators of cellular processes. (elsevier.com)
  • Coupling between inositol 1,4,5-trisphosphate receptors and human transient receptor potential channel 1 when intracellular Ca2+ stores are depleted. (wikipedia.org)
  • 2007). «Inositol 1,4,5-trisphosphate receptors are autoantibody target antigens in patients with Sjögren's syndrome and other systemic rheumatic diseases. (wikipedia.org)
  • 2001). «Alternative splice variants of hTrp4 differentially interact with the C-terminal portion of the inositol 1,4,5-trisphosphate receptors. (wikipedia.org)
  • Evidence that type I, II, and III inositol 1,4,5-trisphosphate receptors can occur as integral plasma membrane proteins. (wikipedia.org)
  • and Miller and Hurley, described the three-dimensional structure of the catalytic region of inositol 1,4,5-trisphosphate 3-kinase (IP 3 -3K), which catalyzes the transfer of phosphate from ATP to the second messenger inositol 1,4,5-trisphosphate (IP 3 ) to produce inositol 1,4,5,6-tetrakisphosphate. (sciencemag.org)
  • These slow imaginal disc intercellular calcium waves (SIDICs) are mediated by the inositol-3-phosphate receptor , the endoplasmic reticulum (ER) calcium pump SERCA and the key gap junction component Inx2 . (sdbonline.org)
  • This material co-eluted from h.p.l.c. with inositol 1,3,4,5 tetrakis[32P]phosphate and is presumably an inositol tetrakisphosphate. (biochemj.org)
  • no inositol 1,4,5-tris[32P]phosphate was produced, suggesting the action of a 5-phosphomonoesterase. (biochemj.org)
  • The half-time of hydrolysis of inositol 1,3,4,5-tetrakis[32P]phosphate of approx. (biochemj.org)
  • Similarly, the rate of production of material tentatively designed as inositol 1,3,4-tris[32P]phosphate from the tetrakisphosphate was reduced by 10 mM-2,3-bisphosphoglyceric acid. (biochemj.org)
  • The ability of 2,3-bisphosphoglyceric acid, an inhibitor of 5-phosphomonoesterase of red blood cell membrane, to inhibit the breakdown of the tetrakisphosphate suggests that the enzyme which removes the 5-phosphate from inositol 1,4,5-trisphosphate may also act to convert the tetrakisphosphate to inositol 1,3,4-trisphosphate. (biochemj.org)
  • Histamine, which produced a larger overall total inositol phosphate response in [H-3]inositol-loaded cells, produced significantly smaller Ins(1,4,5)P3 and Ins(1,3,4,5)P4 responses compared with bradykinin. (lancs.ac.uk)
  • Using Drosophila, an established in vivo model for stress studies, we show that cell-specific inositol phosphate signaling specifically via Inositol 1,4,5 trisphosphate 3-kinase (InsP3 3-K, IP3K), negatively regulates organismal responses to oxidative stress. (gla.ac.uk)
  • Inositol Inositol phosphate Inositol monophosphate Inositol trisphosphate Inositol pentakisphosphate Inositol hexaphosphate MRVI1 associated through complex formation. (wikipedia.org)
  • Controls the cellular levels and subcellular distribution of phosphatidylinositol 3-phosphate and phosphatidylinositol 4,5-bisphosphate. (ymdb.ca)
  • ITP3K catalyzes the transfer of the gamma-phosphate from ATP to the 3-position of inositol 1,4,5-trisphosphate to form inositol 1,3,4,5-tetrakisphosphate. (wikipedia.org)
  • By the end of the decade, a large number of inositol phosphate kinases and phosphatases had been discovered, including ITP3K in 1986. (wikipedia.org)
  • The 4- and 5-phosphate groups of Ins(1,4,5)P3 interact much more extensively than the 1-phosphate. (rcsb.org)
  • The syntheses of four D-myo-inositol 1,4,5-trisphosphate (InsP(3)) derivatives, incorporating phosphate bioisosteres at the 5-position, are reported. (ox.ac.uk)
  • Goutsouliak, V. and Rabkin, S.W. Angiotensin II-induced inositol phosphate generation is mediated through tyrosine kinase pathways in cardiomyocytes. (biomedcentral.com)
  • The transduction mechanism at ORNs is mediated by cAMP and/or inositol,1,4,5-triphosphate (InsP3)-signaling pathways in an odorant-dependent manner. (sdbonline.org)
  • Prolonged stimulation of rat A7r5 aortic smooth muscle cells with 3 microM vasopressin, or of hamster DDT1 MF-2 smooth muscle cells with 10 microM bradykinin or 100 microM histamine led within 4 h to a 40-50% down-regulation of the type 1 InsP3 receptor (InsP3R-1) and of the type 3 InsP3 receptor (InsP3R-3). (nih.gov)
  • The effects of tetrahexyl ammonium cations (THA+) on inositol 1,4,5-trisphosphate-induced calcium release from porcine cerebellar microsomes: THA+ can induce calcium release selectively from the InsP3-sensitive calcium stores. (sigmaaldrich.com)
  • In this study we show that the potassium-channel blocker tetrahexyl ammonium chloride (THA+) is able to inhibit inositol 1,4,5-trisphosphate (InsP3)-induced calcium release in an apparently biphasic fashion with a IC50 of 3 microM. (sigmaaldrich.com)
  • In brain, type I inositol-1,4,5-trisphosphate 5-phosphatase (InsP3 5-phosphatase) is the major isoenzyme hydrolyzing the calcium-mobilizing second messenger InsP3. (semanticscholar.org)
  • The protein encoded by this gene is a membrane-associated type I inositol 1,4,5-trisphosphate (InsP3) 5-phosphatase. (mybiosource.com)
  • InsP3 5-phosphatases hydrolyze Ins(1,4,5)P3, which mobilizes intracellular calcium and acts as a second messenger mediating cell responses to various stimulation. (mybiosource.com)
  • Inositol trisphosphate receptor ( InsP3R ) is a membrane glycoprotein complex acting as a Ca 2+ channel activated by inositol trisphosphate (InsP3). (wikipedia.org)
  • abstract = "The inositol 1,4,5-trisphosphate receptor (InsP3R) is activated by InsP3 binding to amino-terminal ligand binding domain (InsP3R-N). Recently we reported functional coupling of phosphatidylinositol (4,5)-bisphosphate (PIP2) to the InsP3R. (elsevier.com)
  • An Ins(1,4,5)P3 receptor from cerebellum has been purified and functionally reconstituted, but the relationship between this protein and the high-affinity Ins(1,4,5)P3-binding sites of peripheral tissues is unclear. (biochemj.org)
  • We compared the Ins(1,4,5)P3-binding sites of liver and cerebellum by measuring inhibition of specific Ins(1,4,[32P]5)P3 binding by various ligands under equilibrium conditions, and find that each ligand binds with similar affinity in the two tissues. (biochemj.org)
  • We conclude that the high-affinity Ins(1,4,5)P3-binding site of hepatocytes is likely to be the receptor that mediates Ca2+ mobilization, and that this receptor is at present indistinguishable from that in cerebellum. (biochemj.org)
  • Myo-inositol(1,4,5)trisphosphorothioate binds to specific [3H]inositol(1,4,5)trisphosphate sites in rat cerebellum and is resistant to 5-phosphatase. (ox.ac.uk)
  • After the injection of KA in a convulsive dose (10 mg/kg i.p.), inositol 1,4,5-trisphosphate receptor mRNA was reduced significantly in dentate gyrus , cerebral cortex , and caudate- putamen and moderately in CA1-CA3 areas of hippocampus and cerebellum . (bvsalud.org)
  • The significant repression of inositol 1,4,5-trisphosphate receptor mRNA in cerebellum was only found at 9 h after KA injection . (bvsalud.org)
  • Intracellular messenger formed by the action of phospholipase C on phosphatidylinositol 4,5-bisphosphate, which is one of the phospholipids that make up the cell membrane. (jove.com)
  • Activation of PLC-γ induces hydrolysis of phosphatidylinositol-bisphosphate ( 4 , 5 ) to inositol 1,4,5-trisphosphate (IP 3 ) and diacylglycerol ( 15 ). (diabetesjournals.org)
  • The inositol 1,4,5-trisphosphate receptor (InsP 3 R) is activated by InsP 3 binding to amino-terminal ligand binding domain (InsP 3 R-N). Recently we reported functional coupling of phosphatidylinositol (4,5)-bisphosphate (PIP 2 ) to the InsP 3 R. Specific binding of PIP 2 to InsP 3 R-N domain was postulated as a part of the InsP 3 R-PIP 2 functional coupling model. (elsevier.com)
  • Fingerprint Dive into the research topics of 'Association of the inositol (1,4,5)-trisphosphate receptor ligand binding site with phosphatidylinositol (4,5)-bisphosphate and Adenophostin A'. Together they form a unique fingerprint. (elsevier.com)
  • Association of the inositol (1,4,5)-trisphosphate receptor ligand binding site with phosphatidylinositol (4,5)-bisphosphate and Adenophostin A . Molecular Cell Biology Research Communications , 3 (3), 153-158. (elsevier.com)
  • Glouchankova L, Krishna UM, Potter BVL , Falck JR , Bezprozvanny I . Association of the inositol (1,4,5)-trisphosphate receptor ligand binding site with phosphatidylinositol (4,5)-bisphosphate and Adenophostin A . Molecular Cell Biology Research Communications . (elsevier.com)
  • Upon T cell receptor triggering, coronin-1 was essential for the generation of inositol-1,4,5-trisphosphate from phosphatidylinositol-4,5-bisphosphate. (unibas.ch)
  • In previous studies it has been shown that both bradykinin and histamine increase the formation of H-3-labeled inositol phosphates in adrenal chromaffin cells prelabelled with [H-3]inositol and that both these agonists stimulate release of catecholamines by a mechanism dependent on extracellular calcium. (lancs.ac.uk)
  • Ca 2+ release is also isomer specific, with other structurally related inositol phosphates shown to be far less efficacious in releasing Ca 2+ ( 54 ). (pancreapedia.org)
  • Scientific interest in the inositol phosphates intensified in the years following the 1983 discovery that inositol trisphosphate was an intracellular messenger that releases calcium from intracellular stores in the endoplasmic reticulum. (wikipedia.org)
  • The compartmentalization of inositol polyphosphate 5-phosphatase activity presents a possible explanation of earlier findings that premalignant liver tissue was able to respond with inositol 1,4,5-trisphosphate, but not inositol 1,3,4,5-tetrakisphosphate formation after agonist stimulation. (oup.com)
  • The inositol 1,4,5-trisphosphate (IP3)3 receptor (IP3R) are tetrameric intracellular Ca2+ release channels on the endoplasmic membrane that are activated by the ligand IP3 [ PMID: 20813840 ]. (ebi.ac.uk)
  • In mouse eggs, these oscillations cease after a variable period of time and this is accompanied by a decrease in inositol 1,4,5-trisphosphate receptor (IP3R) responsiveness and down-regulation of the IP3R type 1 (IP3R-1). (nih.gov)
  • To investigate the signaling pathway responsible for inducing IP3R-1 down-regulation during fertilization, mouse eggs were exposed to or injected with several Ca(2+)-releasing agonists and the amounts of IP3R-1 immunoreactivity evaluated by Western blotting. (nih.gov)
  • Exposure to ethanol or ionomycin, which induce a single [Ca(2+)]i rise, failed to signal down-regulation of IP3R-1. (nih.gov)
  • However, [Ca(2+)]i oscillations induced by injection of boar sperm fractions (SF), which presumably stimulate production of IP3, or adenophostin A, an IP3R agonist, both induced down-regulation of IP3R-1 of a magnitude similar to or greater than that observed after fertilization. (nih.gov)
  • Exposure to thimerosal, an oxidizing agent that modifies the IP3R without stimulating production of IP3, also initiated down-regulation of IP3R-1, although oscillations initiated by SrCl(2) failed to evoke down-regulation of IP3R-1. (nih.gov)
  • The degradation of IP3R-1 in mouse eggs appears to be mediated by the proteasome pathway because it was inhibited by preincubation with lactacystin, a very specific proteasome inhibitor. (nih.gov)
  • We therefore suggest that persistent stimulation of the phosphoinositide pathway in mouse eggs by the sperm during fertilization or by injection of SF leads to down-regulation of the IP3R-1. (nih.gov)
  • Western blotting using a polyclonal antibody directed against purified brain IP3 receptor (IP3R) identified a specific 260 kD band in 1% Triton X-100 extracts of rat, hamster, mouse and dog sperm. (rupress.org)
  • The presence of G alpha q/11, PLC beta 1 and a functional IP3R in the anterior acrosomal region of mammalian sperm, as well as thapsigargin's induction of the acrosome reaction, implicate IP3-gated calcium release in the mammalian acrosome reaction. (rupress.org)
  • Because the ion conduction pore composes only ∼5% of the IP3R, the great bulk of this large protein contains recognition sites for these substances. (elsevier.com)
  • Sustained Ca2+ entry requires endoplasmic reticulum (ER) Ca2+ depletion and prolonged activation of inositol 1,4,5-trisphosphate receptor (IP3R)/Ca2+ release channels. (elsevier.com)
  • In this study, we examined tear secretion in mice lacking the inositol 1,4,5-trisphosphate receptor (IP3R) types 2 and 3 (Itpr2 -/-;Itpr3-/-double-knockout mice). (elsevier.com)
  • abstract = "The inositol 1,4,5-trisphosphate receptor (IP3R) is a major regulator of apoptotic signaling. (elsevier.com)
  • Phylogenetic analyses were consistent with these proteins representing RyR and IP3R homologues, respectively (Fig. 1). (neuroart2006.com)
  • Number 1 Phylogenetic tree of the RyR and IP3R family members. (neuroart2006.com)
  • Pretreatment with d - myo -inositol-1,4,5-trisphosphate hexasodium ( d - myo -IP 3 ), the sodium salt of the second messenger inositol 1,4,5-trisphosphate (IP 3 ), is cardioprotective and triggers a reduction of infarct size comparable in magnitude to that obtained with ischemic preconditioning. (aspetjournals.org)
  • The ITP3K enzyme family is sometimes confused with a different enzyme family that has a similar name, that is, the phosphatidyl inositol 3-kinases or phosphoinositide 3-kinase (PI3-K),whose substrates are inositol lipids, not the soluble second messenger inositol trisphosphate. (wikipedia.org)
  • Metabolism of inositol 1,4,5-trisphosphate was investigated in permeabilized guinea-pig hepatocytes. (biochemj.org)
  • The metabolism of inositol 1,4,5-trisphosphate and inositol 1,3,4,5-tetrakisphosphate in homogenates and sub-fractions from normal rat liver and premalignant liver nodules was investigated. (oup.com)
  • Ins(1,4,5)P-3 metabolism was examined in Saccharomyces cerevisiae extracts. (dundee.ac.uk)
  • We identified Ca 2+ release through inositol 1,4,5-trisphosphate receptor (InsP 3 R) intracellular channels as a prosurvival target of PKB. (open.ac.uk)
  • The ubiquitous inositol 1,4,5-trisphosphate (InsP 3 ) receptor (InsP 3 R) Ca 2+ release channel plays a central role in the generation and modulation of intracellular Ca 2+ signals, and is intricately regulated by multiple mechanisms including cytoplasmic ligand (InsP 3 , free Ca 2+ , free ATP 4− ) binding, posttranslational modifications, and interactions with cytoplasmic and endoplasmic reticulum (ER) luminal proteins. (rupress.org)
  • The target protein for InsP 3 binding was later identified as the inositol 1,4,5-trisphosphate receptor (InsP 3 R) ( 56 ). (pancreapedia.org)
  • Pancreatic acinar cells are highly polarized, both functionally and morphologically and InsP 3 Rs are predominantly expressed in ER extensions in the apical regions juxtaposed to the acinar lumen ( Figure 1 ) ( 34 , 36 , 42 , 69 ). (pancreapedia.org)
  • Inositol-1,4,5-trisphosphate (InsP 3 ) is a diffusible messenger formed within the cell in response to external stimuli. (springer.com)
  • Here we utilized bacterially expressed and purified InsP 3 R-N domain to characterize its binding specificity for InsP 3 , Adenophostin A (AdA) and the water, soluble PIP 2 analog dioctanoyl-(4,5)PIP 2 (ShPIP 2 ). (elsevier.com)
  • 1 mM, and that for Ins(1,4,5)P-3 was 7.1 mu M. The enzyme appeared to be the first step in the conversion of Ins(1,4,5)P-3 into an InsP(5), and the partially purified preparation contained another activity that converted the InsP(4) product into an InsP(5). (dundee.ac.uk)
  • This activity may be an important step in the production of inositol polyphosphates such as InsP(5) and InsP(6) in S. cerevisiae . (dundee.ac.uk)
  • The results of this study suggest that the origin of the 1,3,4 isomer of inositol trisphosphate in isolated hepatocytes is inositol 1,3,4,5-tetrakisphosphate and that inositol 1,4,5-trisphosphate is rapidly converted to this tetrakisphosphate. (biochemj.org)
  • It is not known if the role of inositol 1,4,5-trisphosphate kinase is to inactivate inositol 1,4,5-trisphosphate or whether the tetrakisphosphate product may have a messenger function in the cell. (biochemj.org)
  • ITP3K is highly specific for the 1,4,5-isomer of IP3, and it exclusively phosphorylates the 3-OH position, producing Ins(1,3,4,5)P4, also known as inositol tetrakisphosphate or IP4. (wikipedia.org)
  • Schild analysis of inhibition of Ins(1,4,5)P3 binding by ATP demonstrates a competitive interaction between the two at the liver Ins(1,4,5)P3-binding site, and this partly accounts for earlier discrepancies in binding and Ca2(+)-release data. (biochemj.org)
  • Enhances Ca(2+)-mediated inhibition of inositol 1,4,5-triphosphate receptor (ITPR) Ca(2+) release. (mybiosource.com)
  • This observation suggests that competitive inhibition of Ins(1,4,5)P3 receptor binding is not an important consideration in cGMP-mediated vascular smooth muscle cell relaxation. (ox.ac.uk)
  • Previously, we indirectly showed that relaxation of porcine coronary artery by adenosine and its analogs are linked to modulation of phospholipase C and protein kinase C. In the present investigation, we assessed if the relaxant responses to the adenosine analogs CAD and NECA are associated with inhibition of inositol 1,4,5-trisphosphate (IP 3 ) formation. (elsevier.com)
  • In the presence of raised K+, 5-hydroxytryptamine caused a rapid, transient inhibition of the K(+)-induced IP3 accumulation, which was maximal after 5 s but had increased to pre-stimulation levels within 30 s in the continued presence of 5-hydroxytryptamine. (ox.ac.uk)
  • The three major potential functions of Ins(1,4,5)P 3 3-kinases are discussed in the context of their localization and regulation, the possible functions of their product, Ins(1,3,4,5)P 4 and their potential contribution to the synthesis of higher inositol polyphosphates. (elsevier.com)
  • Peptide antibodies colocalized G alpha q/11 and the beta 1 isoform of phospholipase C (PLC beta 1) to the anterior acrosomal region of mouse sperm. (rupress.org)
  • Compartmentalization of basal (1,4,5)IP3 was suggested by the fact that this material was stable in the presence of antimycin A, although this toxin completely blocked agonist stimulation of phospholipase C. However, subcellular fractionation as well as permeabilization of the cells with Staphylococcus aureus alpha-toxin failed to provide evidence for binding or sequestration of [3H](1,4,5)IP3 in AR4-2J cells. (biomedsearch.com)
  • The Phospholipase C inhibitor neomycin equally inhibits responses to the two peptides while 5-HT responses are unaffected. (lancs.ac.uk)
  • Both effects of endothelin-1 were blocked by an antagonist of phospholipase C, suggesting that Ins(1,4,5)P-3 and/or diacylglycerol production was necessary. (ucl.ac.uk)
  • The X-ray crystal structure of the high affinity complex between the pleckstrin homology (PH) domain from rat phospholipase C-delta 1 (PLC-delta 1) and inositol-(1,4,5)-trisphosphate (Ins(1,4,5)P3) has been refined to 1.9 A resolution. (rcsb.org)
  • In electrically permeabilized AR4-2J cells, (1,4,5)IP3 and other inositol polyphosphates stimulated Ca2+ release with potencies similar to those reported for other cell types, including the parent pancreatic acinar cell. (biomedsearch.com)
  • Post-translational modification of human brain type I inositol-1,4,5-trisphosphate 5-phosphatase by farnesylation. (semanticscholar.org)
  • Belongs to the inositol 1,4,5-trisphosphate 5- phosphatase type I family. (mybiosource.com)
  • Two analogs with aminocyclohexanecarbonyl (designated as analog 206) or aminobenzoyl group (analog 209) were separated into individual optical isomers and examined for stereospecificity in recognition by IP 3 -5-phosphatase, IP 3 -3-kinase and IP 3 binding activity. (elsevier.com)
  • IP 3 -5-phosphatase activity of erythrocyte ghosts was competitively inhibited by L-209 with a lower K(i) value than D-IP 3 , but with a higher K(i) value by L-206. (elsevier.com)
  • Such being the case, the second hydroxyl group of D-IP 3 may be involved in the recognition by IP 3 -5-phosphatase, but not by IP 3 -3-kinase and binding sites. (elsevier.com)
  • Two analogs with aminocyclohexanecarbonyl (designated as analog 206) or aminobenzoyl group (analog 209) were separated into individual optical isomers and examined for stereospecificity in recognition by IP3-5-phosphatase, IP3-3-kinase and IP3 binding activity. (elsevier.com)
  • IP3-5-phosphatase activity of erythrocyte ghosts was competitively inhibited by L-209 with a lower K(i) value than D-IP3, but with a higher K(i) value by L-206. (elsevier.com)
  • Use of this binding assay has also established that unlike the trisphosphate, the trisphosphorothioate is completely resistant to a specific 5-phosphatase prepared from human erythrocytes. (ox.ac.uk)
  • The activities of 5-phosphatase, expressed as pmol converted substrate per minute and mg protein, were equal when using the two substrates, and did not differ between normal and nodular homogenates. (oup.com)
  • 3-Benzoyl-, 3-methylbenzoyl- and 3-para-aminobenzoyl-Ins(1,4,5)P 3 inhibited the dephosphorylation of [ 3 H]Ins(1,4,5)P 3 by the 5-phosphatase present in erythrocyte ghosts, but the potency varied. (elsevier.com)
  • 3-Benzoyl-, 3-methylbenzoyl- and 3-para-aminobenzoyl-Ins(1,4,5)P3 inhibited the dephosphorylation of [3H]Ins(1,4,5)P3 by the 5-phosphatase present in erythrocyte ghosts, but the potency varied. (elsevier.com)
  • Inositol (1,4,5) trisphosphate 3-kinase (EC 2.7.1.127), abbreviated here as ITP3K, is an enzyme that facilitates a phospho-group transfer from adenosine triphosphate to 1D-myo-inositol 1,4,5-trisphosphate. (wikipedia.org)
  • The present study was intended to investigate the effect of adenosine receptor stimulation on agonist-induced inositol 1,4,5-trisphosphate (IP 3 ) generation in porcine coronary artery using the nonselective adenosine analogs, 2-chloroadenosine (CAD) and 5′-(N-ethylcarboxamido)adenosine (NECA), and the A 2A selective analog 2-p-(2-carboxyethyl)-phenethylamino-5′-N-ethylcarboxamidoadenosine (CGS). (elsevier.com)
  • These proteins mediate a rise in cytoplasmic calcium in response to receptor activated production of inositol triphosphate. (genecards.org)
  • Inositol 1,4,5-trisphosphate (IP 3 ) receptor-associated cyclic GMP (cGMP) kinase substrate (IRAG) is a 125-kD type 2 integral endoplasmic reticulum (ER) membrane protein. (springer.com)
  • The mRNA and protein levels for two rat isoforms of Ins(1,4,5) P 3 3-kinase were determined in the v- src -transformed cell line. (portlandpress.com)
  • Both the mRNA and protein levels for isoform A were elevated in v- src -transformed Rat-1 cells while those for isoform B were not significantly affected. (portlandpress.com)
  • Because a variety of hormones and growth factors activate the Na+/H+ antiporter via protein kinase C and inositol phospholipids, a possible involvement of inositol-1,4,5-trisphosphate (IP3) in the rapid effects of aldosterone in HML was investigated. (uni-muenchen.de)
  • Below are the list of possible Inositol 1,4,5-trisphosphate receptor-interacting protein products. (mybiosource.com)
  • [5] The large size of this open reading frame indicated a molecular weight similar to the protein purified biochemically, and soon thereafter it was confirmed that the protein p400 was in fact the inositol trisphosphate receptor . (wikipedia.org)
  • Because the ion conduction pore composes only ∼5% of the IP 3 R, the great bulk of this large protein contains recognition sites for these substances. (elsevier.com)
  • We show that a previously described Purkinje-cell messenger RNA 6 encodes a protein of relative molecular mass 260,000 (260 K) with the same properties as the cerebellar Ins(l,4,5)P 3 receptor. (elsevier.com)
  • Saturation analysis of isotopic dilution binding data revealed an apparently homogenous population of Ins(1,4,5)P3 binding sites with a KD of 4.02 +/- 0.53 nM and a Bmax of 27.7 +/- 4.6 fmol/mg protein. (ox.ac.uk)
  • The protein encoded by this gene belongs to the inositol 1,4,5-triphosphate receptor family, whose members are second messenger intracellular calcium release channels. (genecards.org)
  • This protein is a type 2 receptor that consists of a cytoplasmic amino-terminus that binds inositol triphosphate, six membrane-spanning helices that contribute to the ion pore, and a short cytoplasmic carboxy-terminus. (genecards.org)
  • ITPR2 (Inositol 1,4,5-Trisphosphate Receptor Type 2) is a Protein Coding gene. (genecards.org)
  • NT-3 induced a rapid increase in calcium ions (Ca 2+ ) in an inositol 1,4,5-trisphosphate (IP 3 )-dependent fashion as well as local activation of the Ca 2+ effector Ca 2+ /calmodulin- dependent protein kinase kinase (CaMKK) in the growth cone. (fujita-hu.ac.jp)
  • NT-3 induced a rapid increase in calcium ions (Ca2+) in an inositol 1,4,5-trisphosphate (IP3)-dependent fashion as well as local activation of the Ca2+ effector Ca2+/calmodulin- dependent protein kinase kinase (CaMKK) in the growth cone. (fujita-hu.ac.jp)
  • In this study we found that the time course of clustering of green fluorescent protein-tagged IP 3 R type 1 (GFP-IP 3 R1), evoked by IP 3 -generating agonists, did not correlate with [Ca 2+ ] C but seemed compatible with cytoplasmic IP 3 concentration. (elsevier.com)
  • We here report that the WD-repeat protein family member coronin-1, encoded by the gene Coro1a, is essential in the mouse for T cell survival through its promotion of Ca2+ mobilization from intracellular stores. (unibas.ch)
  • We produced a plasmid construct expressing the atrial IP3R1 together with green fluorescent protein (GFP), and successfully overexpressed the atrial IP3R1 in the adult atrial cell line HL-1. (biomedcentral.com)
  • [2] Inositol triphosphate receptor represents a dominant second messenger leading to the release of Ca 2+ from intracellular store sites. (wikipedia.org)
  • Inositol triphosphate receptor-mediated signaling is involved in many processes includin. (genecards.org)
  • Inositol triphosphate receptor-mediated signaling is involved in many processes including cell migration, cell division, smooth muscle contraction, and neuronal signaling. (genecards.org)
  • ITPR2 (do inglês, inositol 1,4,5-triphosphate receptor, type 2 ) é uma proteína que é codificado pelo gene humano ITPR2 . (wikipedia.org)
  • Tyr-167/Trp-168 in type 1/3 inositol 1,4,5-trisphosphate receptor mediates functional coupling between ligand binding and channel opening. (ebi.ac.uk)
  • Receptor for inositol 1,4,5-trisphosphate, a second messenger that mediates the release of intracellular calcium. (drugbank.ca)
  • Intracellular channel that mediates calcium release from the endoplasmic reticulum following stimulation by inositol 1,4,5-trisphosphate (PubMed:27108797). (nih.gov)
  • Upon stimulation by inositol 1,4,5-trisphosphate, this receptor mediates calcium release from the endoplasmic reticulum. (nih.gov)
  • A particular aim was to demonstrate if increases in cytoplasmic cyclic guanosine 3':5' monophosphate (cGMP), which mediates the effect of nitrovasodilators, may cause smooth muscle relaxation in part by the displacement of Ins(1,4,5)P3 binding. (ox.ac.uk)
  • The effects of insulin in ob / ob cells could be reproduced by application of a membrane-permeant inositol trisphosphate (IP 3 ) analog and blocked by a frequently used IP 3 receptor inhibitor, 2-aminoethoxydiphenyl borate. (diabetesjournals.org)
  • Incubation of electrically-paced ventricular myocytes with a membrane-permeant Ins(1,4,5)P-3 ester provoked the occurrence of spontaneous diastolic Ca2+ transients with the same characteristics and sensitivity to 2-APB as the events stimulated by endothelin-1. (ucl.ac.uk)
  • Soluble and particulate inositol 1,4,5-trisphosphate 5-phosphatases show common antigenic determinants. (ac.be)
  • In A7r5 cells transiently transfected with a cDNA construct, bearing a luciferase coding sequence under control of the rat InsP3R-1 promoter, reduced luciferase activity could be demonstrated upon stimulation of cells with vasopressin or verapamil. (nih.gov)
  • However, large Ca 2+ signals and/or LTD can also be induced by massive PF stimulation alone or by photolysis of caged Ca 2+ or inositol 1,4,5-trisphosphate (IP 3 ). (jneurosci.org)
  • The bradykinin stimulation of Ins(1,4,5)P3 accumulation was partially dependent on a high (1.8 mM) extracellular Ca2+ concentration, whereas the Ins(1,3,4,5)P4 response was almost completely lost when the extracellular Ca2+ concentration was reduced to 100 nM. (lancs.ac.uk)
  • In addition to evoking spontaneous Ca2+ transients, stimulation of ventricular myocytes with the Ins(1,4,5)P-3 ester caused a positive inotropic effect. (ucl.ac.uk)
  • NSP4 induced a rapid onset and transient stimulation of inositol 1,4,5-trisphosphate (IP 3 ) production in an IP 3 -specific radioreceptor assay. (pnas.org)
  • In the presence of prazosin, 5-HT (300 microM) caused a rapid, transient decrease in IP3 accumulation which was significant after 5 s but had increased to pre-stimulation levels within 15 s. (ox.ac.uk)
  • A series of DL-inositol 1,4,5-trisphosphate (IP 3 ) analogs, with a bulky substituent on the 2nd carbon of the inositol ring, has been synthesized. (elsevier.com)
  • Thus, IP3K-1 modulates epithelial cell apoptosis without involvement of bcl-2-type proteins. (gla.ac.uk)
  • A group of proteins binding to inositol 1,4,5-trisphosphate (IP 3 ) has been identified in rat liver microsomes by a nitrocellulose blot-overlay technique. (elsevier.com)
  • Binding of [ 32 P]IP 3 to these proteins was displaceable to a great extent by 5 μM unlabeled IP 3 but not by 10 μM IP 1 , IP 2 , IP 4 , ATP, or GTPγS. (elsevier.com)
  • Ali, N & Agrawal, DK 1992, ' Liver microsomes contain multiple forms of inositol 1,4,5-trisphosphate binding proteins: Identification by nitrocellulose blot overlay ', Journal of Pharmacological and Toxicological Methods , vol. 27, no. 2, pp. 79-83. (elsevier.com)
  • Agrawal, Devendra K. / Liver microsomes contain multiple forms of inositol 1,4,5-trisphosphate binding proteins : Identification by nitrocellulose blot overlay . (elsevier.com)
  • Binding of [32P]IP3 to these proteins was displaceable to a great extent by 5 μM unlabeled IP3 but not by 10 μM IP1, IP2, IP4, ATP, or GTPγS. (elsevier.com)
  • Characterization of an inositol 1,3,4-trisphosphate 5/6-kinase gene that is essential for drought. (deepdyve.com)
  • Results cDNA Cloning and characterization of and in A total of 12 and 6 overlapping cDNA fragments were acquired for respectively (Table 1). (neuroart2006.com)
  • Here, we show that cultured Purkinje cells from inositol 1,4,5-trisphosphate receptor type 1 knock-out (IP 3 R1KO) mice exhibited abnormal dendritic morphology. (jneurosci.org)
  • Furthermore, double KO mice tended to lose weight rapidly post weaning and typically died 4 weeks after birth. (pancreapedia.org)
  • Specific expression and function of inositol 1,4,5-trisphosphate 3-kinase C (ITPKC) in wild type and knock-out mice. (ac.be)
  • In this study, we examined tear secretion in mice lacking the inositol 1,4,5-trisphosphate receptor (IP 3 R) types 2 and 3 (Itpr2 -/- ;Itpr3 -/- double-knockout mice). (elsevier.com)
  • Earlier studies in which Ins(1,4,5)P3 binding and Ca2+ mobilization were measured under different conditions demonstrated large differences between KD values for binding and EC50 values (concn. (biochemj.org)
  • Spatiotemporal dynamics of inositol 1,4,5-trisphosphate that underlies complex Ca2+ mobilization patterns. (semanticscholar.org)
  • Pretreatment of cells with either 2.3 × 10 −3 units/ml trypsin or 4.4 × 10 −2 units/ml chymotrypsin for 1-10 min abolished the NSP4-induced [Ca 2+ ] i mobilization. (pnas.org)
  • The absence of coronin-1, although it did not affect T cell development, resulted in a profound defect in Ca2+ mobilization, interleukin-2 production, T cell proliferation and T cell survival. (unibas.ch)
  • Endothelin-1 augmented the amplitude of pacing-evoked Ca2+ signals (positive inotropy), and caused an increasing frequency of spontaneous diastolic Ca2+-release transients. (ucl.ac.uk)
  • These results identify IP 3 R as a new regulator of the Beclin 1 complex that may bridge signals converging on the ER and initial phagophore formation. (elsevier.com)
  • Kim, J.C., Son, M.J., Subedi, K.P., Kim, D.H. and Woo, S.H. IP 3 -induced cytosolic and nuclear Ca 2+ signals in HL-1 atrial myocytes: possible role of IP 3 receptor subtypes. (biomedcentral.com)
  • Structural studies of inositol 1,4,5-trisphosphate receptor: coupling ligand binding to channel gating. (ebi.ac.uk)
  • Xestospongin B- or starvation-induced autophagy was inhibited by overexpression of the IP 3 R ligand-binding domain, which coimmunoprecipitated with Beclin 1. (elsevier.com)
  • Ins(1,4,5)P3 binds on the positively charged face of the electrostatically polarized domain, interacting predominantly with the beta 1/beta 2 and beta 3/beta 4 loops. (rcsb.org)
  • However, in the CA1-CA3 areas of the hippocampus , cerebral cortex , and caudate- putamen , the expression of inositol 1,4,5-trisphosphate receptor mRNA was abruptly decreased at 9 h and almost return to basal level at 24 h after KA injection . (bvsalud.org)
  • Inositol 1,4,5-trisphosphate (IP 3 ) is a ubiquitous intracellular messenger regulating diverse functions in almost all mammalian cell types. (open.ac.uk)
  • d-myo-inositol 1,4,5-trisphosphate (Ins(1,4,5)P3, or IP3) is a ubiquitous second messenger that regulates cytosolic Ca2+ activities ([Ca2+]i). (nih.gov)
  • Because inositol trisphosphate (IP 3 ) regulates the release of calcium from the endoplasmic reticulum and the IP 3 kinase A isoform (IP 3 K-A) reduces intracellular IP 3 , regulation of IP 3 K could be involved in neuronal activation and/or neuronal death. (elsevier.com)
  • Release of calcium from inositol 1,4,5-trisphosphate receptor-regulated stores by HIV-1 Tat regulates TNF-alpha production in human macrophages. (wikipedia.org)
  • In this study, we found the specific expression of type 2 inositol 1,4,5-trisphosphate receptor (IP 3 R2), which is an intracellular Ca 2+ release channel, on the sarco/endoplasmic reticulum in mouse PASMCs, and demonstrated its inhibitory role in the progression of PAH using a chronic hypoxia-induced PAH mouse model. (springer.com)
  • To study whether sarcoplasmic/endoplasmic reticulum Ca 2+ -ATPase (SERCA)-type Ca 2+ -ATPases are responsible for Ca 2+ uptake into Ins(1,4,5) P 3 -sensitive Ca 2+ stores, we used the three structurally unrelated inhibitors thapsigargin, 2,5-di-t-butylhydroquinone and cyclopiazonic acid. (portlandpress.com)
  • Inositol 1,4,5-trisphosphate directs Ca(2+) flow between mitochondria and the Endoplasmic/Sarcoplasmic. (unige.ch)
  • Sustained Ca 2+ entry requires endoplasmic reticulum (ER) Ca 2+ depletion and prolonged activation of inositol 1,4,5-trisphosphate receptor (IP 3 R)/Ca 2+ release channels. (elsevier.com)
  • We have characterized inositol 1,4,5-trisphosphate (IP3)-gated calcium channels and upstream components of the phosphoinositide signaling system in mammalian sperm. (rupress.org)
  • Expression of the cDNA in COS cells resulted in an approximately 150-fold increase in inositol 1,4,5-trisphosphate 3-kinase activity of these cells. (sciencemag.org)
  • In this study, we show that actin dynamics in spines is regulated by a Rac anchoring and targeting function of inositol 1,4,5-trisphosphate 3-kinase A (IP 3 K-A), independent of its kinase activity. (pubmedcentralcanada.ca)
  • Inositol 1,4,5-trisphosphate 3-kinase activity was found to be exclusively cytosolic. (oup.com)
  • S. cerevisiae contains readily detectable Ins(1,4,5)P-3 kinase activity that is predominantly soluble, but phosphomonoesterase activity acting on Ins(1,4,5)P-3 was not detected in either soluble or particulate preparations from this organism. (dundee.ac.uk)
  • FLRFamide and FMRFamide induced small depolarizations (1-2 mV) which triggered a burst of action potentials of about 5 mV which on reaching 4 mV triggered a burst of fast twitch contractions. (lancs.ac.uk)
  • The mutant phenotype was caused by a T-DNA insertion in a gene encoding a putative inositol 1,3,4-trisphosphate 5/6-kinase previously named OsITPK2 with unknown function. (deepdyve.com)
  • This gene encodes an intracellular receptor for inositol 1,4,5-trisphosphate. (nih.gov)
  • Note however, that the human genome also contains a gene for a different kinase known as ITPK1, which is an inositol 1, 3, 4-trisphosphate 5/6-kinase and is not a member of the IPK family. (wikipedia.org)
  • [ 1 ] A proteína codificada por este gene é um receptor para o inositol trifosfato e um canal de cálcio. (wikipedia.org)
  • 1 Department of Cell Biology, University of Texas Southwestern Medical Center, 5323 Harry Hines Blouvard, Dallas, TX 75390-9039, United States. (nih.gov)
  • Journal of Cell Biology , 179 (5), 923-934. (elsevier.com)
  • Recently, we described a novel autoantibody, anti-Sj/ITPR1-IgG, that targets the inositol 1,4,5-trisphosphate receptor type 1 (ITPR1) in patients with cerebellar ataxia. (biomedcentral.com)
  • D-Myo-inositol(1,4,5)trisphosphorothioate, a synthetic analogue of inositol(1,4,5)trisphosphate was shown to bind with a relatively high affinity to specific sites on rat cerebellar membranes labelled with [3H]inositol(1,4,5)trisphosphate. (ox.ac.uk)
  • A cerebellar Ins(l,4,5)P 3 receptor has been characterized biochemically 3,4 and shown by immunocytochemistry to be present in intracellular membranes in Purkinje cells 5 . (elsevier.com)
  • They also decreased, in parallel, ATP-dependent Ca 2+ accumulation and the amount of Ins(1,4,5) P 3 -releasable Ca 2+ . (portlandpress.com)
  • Prostaglandins mediate the stimulatory effects of endothelin-1 on cAMP accumulation and inositol-1,4,5-trisphosphate production and contraction in cat iris sphincter. (aspetjournals.org)
  • Kinetic studies revealed the following: (1) The effect of ET-1 on cAMP accumulation is rapid (within 30 sec), dose dependent (EC50 = 5.8 nM) and completely abolished by Indo (Ki = 0.16 microM), a cyclooxygenase inhibitor, but not by nordihydroguairetic acid, a lipoxygenase inhibitor, implying the involvement of PGs. (aspetjournals.org)
  • We conclude from these data that in cat iris sphincter PGs may mediate ET-1-induced cAMP accumulation, IP3 production and smooth muscle contraction. (aspetjournals.org)
  • Specific accumulation of inositol 1,4,5-trisphosphate in rabbit basilar artery in response to noradrenaline but not 5-hydroxytryptamine. (ox.ac.uk)
  • This study examined the ability of 5-hydroxytryptamine and noradrenaline to stimulate inositol 1,4,5-trisphosphate (IP3) mass accumulation in segments of the rabbit basilar artery. (ox.ac.uk)
  • 5-Hydroxytryptamine (5-HT, 100 microM) failed to stimulate any significant accumulation of IP3 during the 5 min period following its application. (ox.ac.uk)
  • In contrast, noradrenaline (10 microM) stimulated a rapid, transient accumulation of IP3 which was significant after 5 s but had declined to basal levels after 60 s. (ox.ac.uk)
  • The IP3 accumulation stimulated by either 5-HT or raised K+ was not reduced by the presence of the alpha 1-adrenoceptor antagonist, prazosin (0.1 microM). (ox.ac.uk)
  • Type 1 IP 3 R (IP 3 R1) is enriched in growth cones of neurons in chick dorsal root ganglia. (sciencemag.org)
  • Obesity and type 2 diabetes are leading causes of coronary heart disease and heart failure ( 1 - 4 ), and clinical and experimental studies have shown that diabetes is associated with altered cardiac function independent of vascular complications ( 5 , 6 ). (diabetesjournals.org)
  • Immunoblotting with subtype-directed antibodies demonstrated that HL-60 cells contain the Ca 2+ pump SERCA2 (subtype b), and the Ca 2+ -release-channel type-1 Ins(1,4,5) P 3 receptor. (portlandpress.com)
  • In subcellular fractionation studies, SERCA2 and type-1 Ins(1,4,5) P 3 receptor co-purified. (portlandpress.com)
  • Immunofluorescence studies demonstrated that both type-1 Ins(1,4,5) P 3 receptor and SERCA2 were evenly distributed throughout the cell in moving neutrophils. (portlandpress.com)
  • Taken together, our results suggest that in myeloid cells (i) SERCA-type Ca 2+ -ATPases function as Ca 2+ pumps of Ins(1,4,5) P 3 -sensitive Ca 2+ stores, and (ii) SERCA2 and type-1 Ins(1,4,5) P 3 receptor reside either in the same or two tightly associated subcellular compartments. (portlandpress.com)
  • We demonstrate that the Drosophila Malpighian tubule (equivalent to vertebrate kidney and liver) is a key epithelial sensor for organismal oxidative stress responses: precise targeting of either gain-of-function constructs of Drosophila IP3Ks (IP3K-1 and IP3K-2), or loss-of-function (RNAi) constructs to only one cell type in tubule reversibly modulates survival of stress-challenged adult flies. (gla.ac.uk)
  • Sitting in patients with spinal muscular atrophy type 1 treated with nusinersen. (ac.be)
  • Inositol 1,4,5-trisphosphate receptor type 1 (IP 3 R1) is already known to be highly expressed in the brain, and is found in many other tissues, including the atrium of the heart. (biomedcentral.com)
  • Activation of store-mediated calcium entry by secretion-like coupling between the inositol 1,4,5-trisphosphate receptor type II and human transient receptor potential (hTrp1) channels in human platelets. (wikipedia.org)
  • The effects of endothelin-1 were compared with two other stimuli, isoproterenol and digoxin, which are known to induce inotropy and spontaneous Ca2+ transients by overloading intracellular Ca2+ stores. (ucl.ac.uk)
  • Inositol 1,4,5-trisphosphate turnover enzymes--activities and subcellular distribution in hepatocarcinogenesis. (oup.com)
  • To evaluate the contribution of gap junctions/hemichannels, additional control and d - myo -IP 3 -treated cohorts received a 5-min infusion of heptanol or Gap 27, two structurally distinct gap junction inhibitors, administered at doses confirmed to attenuate intercellular transmission of a gap junction-permeable fluorescent dye. (aspetjournals.org)
  • abstract = "The inositol 1,4,5 trisphosphate (IP3) receptor (IP 3R) is a Ca2+ release channel that responds to the second messenger IP3. (elsevier.com)
  • abstract = "It is 20 yr since the Ins(1,4,5)P3 3-kinase reaction was discovered, and this review summarizes and discusses some of the advances we have made in our understanding of the physiological significance of this reaction. (elsevier.com)
  • abstract = "Inositol 1,4,5-trisphosphate (IP3) is generally viewed as a global messenger that increases cytosolic calcium ion (Ca2+) concentration. (elsevier.com)
  • abstract = "d-myo-Inositol 1,4,5-trisphosphate (Ins(1,4,5)P3) analogues derived at 3-OH with a bulky substituent were chemically synthesized and structural features of vicinity surrounding the 3-OH of Ins(1,4,5)P3, recognized by metabolic enzymes and by the receptor were explored. (elsevier.com)
  • abstract = "The inositol 1,4,5-trisphosphate (IP3) receptor (IP 3R) Ca2+ channel plays pivotal roles in many aspects of physiological and pathological events. (elsevier.com)
  • abstract = "We compared the effect of platelet-activating factor (PAF) on inositol 1,4,5-trisphosphate (IP3) content in undifferentiated and differentiated U937 cells. (elsevier.com)
  • INOSITOL 1,4,5-trisphosphate (Ins(l,4,5)P 3 ) serves as an intracellular second messenger for several neurotransmitters, hormones and growth factors by initiating calcium release from intracellular stores 1,2 . (elsevier.com)
  • NSP4 (1 nM to 5 μM) induced both Ca 2+ release from intracellular stores and plasmalemma Ca 2+ influx. (pnas.org)
  • We conclude that coronin-1, through activation of Ca2+ release from intracellular stores, is an essential regulator of peripheral lymphocyte survival. (unibas.ch)
  • InsP3R-associated cGMP kinase substrate determines inositol 1,4,5-trisphosphate receptor susceptibility to phosphoregulation by cyclic nucleotide-dependent kinases. (springer.com)
  • In contrast, cyclic guanosine 3':5' monophosphate (cGMP) did not significantly alter Ins(1,4,5)P3 binding in rabbit aortic smooth muscle. (ox.ac.uk)