An annual legume. The SEEDS of this plant are edible and used to produce a variety of SOY FOODS.
Mapping of the KARYOTYPE of a cell.
A type of IN SITU HYBRIDIZATION in which target sequences are stained with fluorescent dye so their location and size can be determined using fluorescence microscopy. This staining is sufficiently distinct that the hybridization signal can be seen both in metaphase spreads and in interphase nuclei.
Mapping of the linear order of genes on a chromosome with units indicating their distances by using methods other than genetic recombination. These methods include nucleotide sequencing, overlapping deletions in polytene chromosomes, and electron micrography of heteroduplex DNA. (From King & Stansfield, A Dictionary of Genetics, 5th ed)
Widely used technique which exploits the ability of complementary sequences in single-stranded DNAs or RNAs to pair with each other to form a double helix. Hybridization can take place between two complimentary DNA sequences, between a single-stranded DNA and a complementary RNA, or between two RNA sequences. The technique is used to detect and isolate specific sequences, measure homology, or define other characteristics of one or both strands. (Kendrew, Encyclopedia of Molecular Biology, 1994, p503)
A technique for visualizing CHROMOSOME ABERRATIONS using fluorescently labeled DNA probes which are hybridized to chromosomal DNA. Multiple fluorochromes may be attached to the probes. Upon hybridization, this produces a multicolored, or painted, effect with a unique color at each site of hybridization. This technique may also be used to identify cross-species homology by labeling probes from one species for hybridization with chromosomes from another species.
Any method used for determining the location of and relative distances between genes on a chromosome.
Hospital department which administers and provides pathology services.
A publication issued at stated, more or less regular, intervals.
Individual's rights to obtain and use information collected or generated by others.
A subspecialty of pathology applied to the solution of clinical problems, especially the use of laboratory methods in clinical diagnosis. (Dorland, 28th ed.)
A specialty concerned with the nature and cause of disease as expressed by changes in cellular or tissue structure and function caused by the disease process.
A quantitative measure of the frequency on average with which articles in a journal have been cited in a given period of time.
Embryonic (precursor) cells of the myogenic lineage that develop from the MESODERM. They undergo proliferation, migrate to their various sites, and then differentiate into the appropriate form of myocytes (MYOCYTES, SKELETAL; MYOCYTES, CARDIAC; MYOCYTES, SMOOTH MUSCLE).
A group of cold-blooded, aquatic vertebrates having gills, fins, a cartilaginous or bony endoskeleton, and elongated bodies covered with scales.
Therapy using oral or topical photosensitizing agents with subsequent exposure to light.
The property of emitting radiation while being irradiated. The radiation emitted is usually of longer wavelength than that incident or absorbed, e.g., a substance can be irradiated with invisible radiation and emit visible light. X-ray fluorescence is used in diagnosis.
Drugs that are pharmacologically inactive but when exposed to ultraviolet radiation or sunlight are converted to their active metabolite to produce a beneficial reaction affecting the diseased tissue. These compounds can be administered topically or systemically and have been used therapeutically to treat psoriasis and various types of neoplasms.
Measurement of the intensity and quality of fluorescence.
A technique that localizes specific nucleic acid sequences within intact chromosomes, eukaryotic cells, or bacterial cells through the use of specific nucleic acid-labeled probes.
A bibliographic database that includes MEDLINE as its primary subset. It is produced by the National Center for Biotechnology Information (NCBI), part of the NATIONAL LIBRARY OF MEDICINE. PubMed, which is searchable through NLM's Web site, also includes access to additional citations to selected life sciences journals not in MEDLINE, and links to other resources such as the full-text of articles at participating publishers' Web sites, NCBI's molecular biology databases, and PubMed Central.
"The business or profession of the commercial production and issuance of literature" (Webster's 3d). It includes the publisher, publication processes, editing and editors. Production may be by conventional printing methods or by electronic publishing.
A nevus containing melanin. The term is usually restricted to nevocytic nevi (round or oval collections of melanin-containing nevus cells occurring at the dermoepidermal junction of the skin or in the dermis proper) or moles, but may be applied to other pigmented nevi.
Tumors or cancer of the CONJUNCTIVA.
Disorders of increased melanin pigmentation that develop without preceding inflammatory disease.
A malignant neoplasm derived from cells that are capable of forming melanin, which may occur in the skin of any part of the body, in the eye, or, rarely, in the mucous membranes of the genitalia, anus, oral cavity, or other sites. It occurs mostly in adults and may originate de novo or from a pigmented nevus or malignant lentigo. Melanomas frequently metastasize widely, and the regional lymph nodes, liver, lungs, and brain are likely to be involved. The incidence of malignant skin melanomas is rising rapidly in all parts of the world. (Stedman, 25th ed; from Rook et al., Textbook of Dermatology, 4th ed, p2445)
A circumscribed stable malformation of the skin and occasionally of the oral mucosa, which is not due to external causes and therefore presumed to be of hereditary origin.
Tumors or cancer of the SKIN.
Clinically atypical nevi (usually exceeding 5 mm in diameter and having variable pigmentation and ill defined borders) with an increased risk for development of non-familial cutaneous malignant melanoma. Biopsies show melanocytic dysplasia. Nevi are clinically and histologically identical to the precursor lesions for melanoma in the B-K mole syndrome. (Stedman, 25th ed)
A benign compound nevus occurring most often in children before puberty, composed of spindle and epithelioid cells located mainly in the dermis, sometimes in association with large atypical cells and multinucleate cells, and having a close histopathological resemblance to malignant melanoma. The tumor presents as a smooth to slightly scaly, round to oval, raised, firm papule or nodule, ranging in color from pink-tan to purplish red, often with surface telangiectasia. (Dorland, 27th ed)
A cellular subtype of malignant melanoma. It is a pigmented lesion composed of melanocytes occurring on sun-exposed skin, usually the face and neck. The melanocytes are commonly multinucleated with a "starburst" appearance. It is considered by many to be the in situ phase of lentigo maligna melanoma.
A cancer registry mandated under the National Cancer Act of 1971 to operate and maintain a population-based cancer reporting system, reporting periodically estimates of cancer incidence and mortality in the United States. The Surveillance, Epidemiology, and End Results (SEER) Program is a continuing project of the National Cancer Institute of the National Institutes of Health. Among its goals, in addition to assembling and reporting cancer statistics, are the monitoring of annual cancer incident trends and the promoting of studies designed to identify factors amenable to cancer control interventions. (From National Cancer Institute, NIH Publication No. 91-3074, October 1990)
Mammalian pigment cells that produce MELANINS, pigments found mainly in the EPIDERMIS, but also in the eyes and the hair, by a process called melanogenesis. Coloration can be altered by the number of melanocytes or the amount of pigment produced and stored in the organelles called MELANOSOMES. The large non-mammalian melanin-containing cells are called MELANOPHORES.
Nucleic acid which complements a specific mRNA or DNA molecule, or fragment thereof; used for hybridization studies in order to identify microorganisms and for genetic studies.
Erbium. An element of the rare earth family of metals. It has the atomic symbol Er, atomic number 68, and atomic weight 167.26.
A telecommunication system combining the transmission of a document scanned at a transmitter, its reconstruction at a receiving station, and its duplication there by a copier.
A province of Canada on the Pacific coast. Its capital is Victoria. The name given in 1858 derives from the Columbia River which was named by the American captain Robert Gray for his ship Columbia which in turn was named for Columbus. (From Webster's New Geographical Dictionary, 1988, p178 & Room, Brewer's Dictionary of Names, 1992, p81-2)

11q23.1 and 11q25-qter YACs suppress tumour growth in vivo. (1/9173)

Frequent allelic deletion at chromosome 11q22-q23.1 has been described in breast cancer and a number of other malignancies, suggesting putative tumour suppressor gene(s) within the approximately 8 Mb deleted region. In addition, we recently described another locus, at the 11q25-qter region, frequently deleted in breast cancer, suggesting additional tumour suppressor gene(s) in this approximately 2 Mb deleted region. An 11q YAC contig was accessed and three YACs, one containing the candidate gene ATM at 11q23.1, and two contiguous YACs (overlapping for approximately 400-600 kb) overlying most of the 11q25 deleted region, were retrofitted with a G418 resistance marker and transfected into murine A9 fibrosarcoma cells. Selected A9 transfectant clones (and control untransfected and 'irrelevant' alphoid YAC transfectant A9 clones) were assayed for in vivo tumorigenicity in athymic female Balb c-nu/nu mice. All the 11q YAC transfectant clones demonstrated significant tumour suppression compared to the control untransfected and 'irrelevant' YAC transfected A9 cells. These results define two discrete tumour suppressor loci on chromosome 11q by functional complementation, one to a approximately 1.2 Mb region on 11q23.1 (containing the ATM locus) and another to a approximately 400-600 kb subterminal region on 11q25-qter.  (+info)

Deletion of a region that is a candidate for the difference between the deletion forms of hereditary persistence of fetal hemoglobin and deltabeta-thalassemia affects beta- but not gamma-globin gene expression. (2/9173)

The analysis of a number of cases of beta-globin thalassemia and hereditary persistence of fetal hemoglobin (HPFH) due to large deletions in the beta-globin locus has led to the identification of several DNA elements that have been implicated in the switch from human fetal gamma- to adult beta-globin gene expression. We have tested this hypothesis for an element that covers the minimal distance between the thalassemia and HPFH deletions and is thought to be responsible for the difference between a deletion HPFH and deltabeta-thalassemia, located 5' of the delta-globin gene. This element has been deleted from a yeast artificial chromosome (YAC) containing the complete human beta-globin locus. Analysis of this modified YAC in transgenic mice shows that early embryonic expression is unaffected, but in the fetal liver it is subject to position effects. In addition, the efficiency of transcription of the beta-globin gene is decreased, but the developmental silencing of the gamma-globin genes is unaffected by the deletion. These results show that the deleted element is involved in the activation of the beta-globin gene perhaps through the loss of a structural function required for gene activation by long-range interactions.  (+info)

Analysis of genomic integrity and p53-dependent G1 checkpoint in telomerase-induced extended-life-span human fibroblasts. (3/9173)

Life span determination in normal human cells may be regulated by nucleoprotein structures called telomeres, the physical ends of eukaryotic chromosomes. Telomeres have been shown to be essential for chromosome stability and function and to shorten with each cell division in normal human cells in culture and with age in vivo. Reversal of telomere shortening by the forced expression of telomerase in normal cells has been shown to elongate telomeres and extend the replicative life span (H. Vaziri and S. Benchimol, Curr. Biol. 8:279-282, 1998; A. G. Bodnar et al., Science 279:349-352, 1998). Extension of the life span as a consequence of the functional inactivation of p53 is frequently associated with loss of genomic stability. Analysis of telomerase-induced extended-life-span fibroblast (TIELF) cells by G banding and spectral karyotyping indicated that forced extension of the life span by telomerase led to the transient formation of aberrant structures, which were subsequently resolved in higher passages. However, the p53-dependent G1 checkpoint was intact as assessed by functional activation of p53 protein in response to ionizing radiation and subsequent p53-mediated induction of p21(Waf1/Cip1/Sdi1). TIELF cells were not tumorigenic and had a normal DNA strand break rejoining activity and normal radiosensitivity in response to ionizing radiation.  (+info)

Tissue specific expression and chromosomal mapping of a human UDP-N-acetylglucosamine: alpha1,3-d-mannoside beta1, 4-N-acetylglucosaminyltransferase. (4/9173)

A human cDNA for UDP- N -acetylglucosamine:alpha1,3-d-mannoside beta1,4- N- acetylglucosaminyltransferase (GnT-IV) was isolated from a liver cDNA library using a probe based on a partial cDNA sequence of the bovine GnT-IV. The cDNA encoded a complete sequence of a type II membrane protein of 535 amino acids which is 96% identical to the bovine GnT-IV. Transient expression of the human cDNA in COS7 cells increased total cellular GnT-IV activity 25-fold, demonstrating that this cDNA encodes a functional human GnT-IV. Northern blot analysis of normal tissues indicated that at least five different sizes of mRNA (9.7, 7.6, 5.1, 3.8, and 2.4 kb) forGnT-IV are expressed in vivo. Furthermore, these mRNAs are expressed at different levels between tissues. Large amounts of mRNA were detected in tissues harboring T lineage cells. Also, the promyelocytic leukemia cell line HL-60 and the lymphoblastic leukemia cell line MOLT-4 revealed abundant mRNA. Lastly, the gene was mapped at the locus on human chromosome 2, band q12 by fluorescent in situ hybridization.  (+info)

Insertion of excised IgH switch sequences causes overexpression of cyclin D1 in a myeloma tumor cell. (5/9173)

Oncogenes are often dysregulated in B cell tumors as a result of a reciprocal translocation involving an immunoglobulin locus. The translocations are caused by errors in two developmentally regulated DNA recombination processes: V(D)J and IgH switch recombination. Both processes share the property of joining discontinuous sequences from one chromosome and releasing intervening sequences as circles that are lost from progeny cells. Here we show that these intervening sequences may instead insert in the genome and that during productive IgH mu-epsilon switch recombination in U266 myeloma tumor cells, a portion of the excised IgH switch intervening sequences containing the 3' alpha-1 enhancer has inserted on chromosome 11q13, resulting in overexpression of the adjacent cyclin D1 oncogene.  (+info)

Specific chromosomal aberrations and amplification of the AIB1 nuclear receptor coactivator gene in pancreatic carcinomas. (6/9173)

To screen pancreatic carcinomas for chromosomal aberrations we have applied molecular cytogenetic techniques, including fluorescent in situ hybridization, comparative genomic hybridization, and spectral karyotyping to a series of nine established cell lines. Comparative genomic hybridization revealed recurring chromosomal gains on chromosome arms 3q, 5p, 7p, 8q, 12p, and 20q. Chromosome losses were mapped to chromosome arms 8p, 9p, 17p, 18q, 19p, and chromosome 21. The comparison with comparative genomic hybridization data from primary pancreatic tumors indicates that a specific pattern of chromosomal copy number changes is maintained in cell culture. Metaphase chromosomes from six cell lines were analyzed by spectral karyotyping, a technique that allows one to visualize all chromosomes simultaneously in different colors. Spectral karyotyping identified multiple chromosomal rearrangements, the majority of which were unbalanced. No recurring reciprocal translocation was detected. Cytogenetic aberrations were confirmed using fluorescent in situ hybridization with probes for the MDR gene and the tumor suppressor genes p16 and DCC. Copy number increases on chromosome 20q were validated with a probe specific for the nuclear receptor coactivator AIB1 that maps to chromosome 20q12. Amplification of this gene was identified in six of nine pancreatic cancer cell lines and correlated with increased expression.  (+info)

Treponema brennaborense sp. nov., a novel spirochaete isolated from a dairy cow suffering from digital dermatitis. (7/9173)

A novel Treponema species was isolated from an ulcerative lesion of a cow suffering from digital dermatitis (DD), a disease which causes painful ulcerations along the coronary band. Among other anaerobic bacteria, high numbers of spirochaetes have been regularly found in DD lesions. Here data are presented of a spirochaete isolated from a DD ulcer. By chemotaxonomy, protein analysis and comparative 16S rDNA sequence analysis this isolate was classified as a treponeme that differed from all Treponema species described previously. The only isolate, DD5/3T, for which the name Treponema brennaborense is proposed, is designated the type strain of the novel species. The strain is a small, highly motile spirochaete that has two periplasmic flagella, one flagellum being attached at each cell pole. Strain DD5/3T exhibits alpha-glucosidase and N-acetyl-beta-glucosaminidase activity and growth is inhibited by rabbit serum. T. brennaborense was phylogenetically most closely related (89.5% 16S rRNA similarity) to Treponema maltophilum, an oral spirochaete isolated from a periodontitis patient.  (+info)

Survey of gene amplifications during prostate cancer progression by high-throughout fluorescence in situ hybridization on tissue microarrays. (8/9173)

Prostate cancer development and progression is driven by the accumulation of genetic changes, the nature of which remains incompletely understood To facilitate high-throughput analysis of molecular events taking place in primary, recurrent, and metastat prostate cancer, we constructed a tissue microarray containing small 0.6-mm cylindrical samples acquired from 371 formalin-fixed blocks, including benign prostatic hyperplasia (n = 32) and primary tumors (n = 223), as well as both locally recurrent tumors (n = 54) and metastases (n = 62) from patients with hormone-refractory disease. Fluorescence in situ hybridization (FISH) was applied to the analysis of consecutive tissue microarray sections with probes for five different genes. High-level (> or =3X) amplifications were very rare (<2%) in primary prostate cancers However, in metastases from patients with hormone-refractory disease, amplification of the androgen receptor gene was seen in 22%, MYC in 11%, and Cyclin-D1 in 5% of the cases. In specimens from locally recurrent tumors, the corresponding percentages were 23, 4, and 8%. ERBB2 and NMYC amplifications were never detected at any stage of prostate cancer progression. In conclusion, FISH to tissue microarray sections enables high-throughput analysis of genetic alterations contributing to cancer development and progression. Our results implicate a role for amplification of androgen receptor in hormonal therapy failure and that of MYC in the metastatic progression of human prostate cancer.  (+info)

TY - JOUR. T1 - High-speed dual color fluorescence lifetime endomicroscopy for highly-multiplexed pulmonary diagnostic applications and detection of labeled bacteria. AU - Pedretti, Ettore. AU - Tanner, Michael G.. AU - Choudhary, Tushar R. AU - Krstajic, Nikola. AU - Megia-Fernandez, Alicia. AU - Henderson, Robert K.. AU - Bradley, Mark. AU - Thomson, Robert R.. AU - Girkin, John M.. AU - Dhaliwal, Kevin. AU - Dalgarno, Paul A.. PY - 2019/1/1. Y1 - 2019/1/1. N2 - We present a dual-color laser scanning endomicroscope capable of fluorescence lifetime endomicroscopy at one frame per second (FPS). The scanning system uses a coherent imaging fiber with 30,000 cores. High-speed lifetime imaging is achieved by distributing the signal over an array of 1024 parallel single-photon avalanche diode detectors (SPADs), minimizing detection dead-time maximizing the number of photons detected per excitation pulse without photon pile-up to achieve the high frame rate. This also enables dual color fluorescence ...
RESULTS. Fluorescence in-situ hybridisation detected 558 (9.5%) patients with chromosomal abnormalities. Abnormal ultrasounds (70%) and maternal serum screens (21%) were the most indicative of chromosomal abnormalities. When comparing fluorescence in-situ hybridisation data with karyotype results for the five chromosomes of interest, the sensitivity and specificity were 99.3% and 99.9%, respectively. When comparing fluorescence in-situ hybridisation data with karyotype results for all chromosomes, the sensitivity decreased to 86.8%, whereas the specificity remained at 99.9%. Of 643 cases with karyotype abnormalities, 85 were fluorescence in-situ hybridisation-negative (false negative rate, 13.2%), which included structural rearrangements, chromosome mosaicism, and other trisomies. Despite abnormal ultrasound indications, fluorescence in-situ hybridisation missed 32 cases which included structural rearrangements, mosaicisms, and other trisomies ...
16120DNAArtificial SequenceYM-1 Forward Primer 1tggaattggt gcccctacaa 20220DNAArtificial SequenceYM-1 Reverse Primer 2aacttgcact gtgtatattg 20318DNAArtificial SequenceYM-2 Forward Primer 3aacctcagac attcatta 18421DNAArtificial SequenceYM-2 Reverse Primer 4tggtccttcc agtaggtaat a 21520DNAArtificial SequenceYM-3 Forward Primer 5tataaatctc catttgacac 20620DNAArtificial SequenceYM-3 Reverse Primer 6cctaatttat tgtccttgac 20728DNAArtificial SequenceAMCase Forward Primer 7atctgcagtg gacacacctt catcctga 28828DNAArtificial SequenceAMCase Reverse Primer 8atgaattcaa caagccctgc ttgacaat 28922DNAArtificial SequenceYM Antisense In Situ Hybridization Probe 9tcctcgagac ccagggtact gc 221024DNAArtificial SequenceYM Sense In Situ Hybridization Probe 10tatctagagg atcttcctac cagc 241129DNAArtificial SequenceAMCase Antisense In Situ Hybridization Probe 11tcgctcgaga acaagccctg cttgacaat 291228DNAArtificial SequenceAMCase sense In Situ Hybridization Probe 12gctctagatg gacacacctt catcctga 281319PRTArtificial ...
TY - JOUR. T1 - Chromosome microdissection. T2 - A brief overview. AU - Cannizzaro, L. A.. PY - 1996/1/1. Y1 - 1996/1/1. N2 - Chromosome microdissection arose as a means of facilitating long range physical mapping of chromosome regions involved in either a genetic or malignant disorder. However, with the rapid development of improved techniques for mapping and sequencing the human genome, microdissection is considered by many investigators to be a cumbersome and time consuming procedure. Nonetheless, based on the impressive number of informative diagnostic DNA markers that are now available as a result of this technology, microdissection still must be considered one of the most rapid and direct methods available for generating new DNA markers from any chromosome region, irrespective of its sequence composition. In addition, it remains an important means to dissect DNA markers from any organism, eukaryotic and prokaryotic, and has resulted in generating disease associated DNA sequences from both ...
TY - JOUR. T1 - Molecular cytogenetic study and chromosome abnormalities in Alzheimers disease. Protease-inhibitory peptides and amyloid beta protein in Alzheimers disease. AU - Ito, H.. AU - Kitaguchi, N.. PY - 1988/7/1. Y1 - 1988/7/1. UR - http://www.scopus.com/inward/record.url?scp=0024046766&partnerID=8YFLogxK. UR - http://www.scopus.com/inward/citedby.url?scp=0024046766&partnerID=8YFLogxK. M3 - Article. C2 - 3065525. AN - SCOPUS:0024046766. VL - 46. SP - 1514. EP - 1520. JO - Nippon rinsho. Japanese journal of clinical medicine. JF - Nippon rinsho. Japanese journal of clinical medicine. SN - 0047-1852. IS - 7. ER - ...
TY - JOUR. T1 - Eigenanalysis of DAPI-stained chromosomes. T2 - Tools and strategies toward computer-assisted analysis of FISH experiments. AU - Knapp, R. D.. AU - Smith, L. C.. AU - Baldini, A.. PY - 1995. Y1 - 1995. N2 - The fluorescent dye 4,6-diamidino-2-phenylindole (DAPI) is widely used as a chromosome counterstain in fluorescence in situ hybridization (FISH) studies. It produces a Q-banding pattern that allows for both chromosome identification and the assignment of molecular probes to specific chromosome bands. Using a statistical procedure based on eigenanalysis, we have extracted features from digital images of DAPI-stained chromosomes and constructed prototypes of each of the 24 human chromosomes. The features of these prototypes are directly proportional, in intensity profile and band location, to those of real chromosomes. The prototypes intensity profile can be translated into cytogenetic bands to provide a computer-based strategy for chromosome mapping and analysis amenable to ...
Before Its News). Global FISH Probe Market 2016-2020 Order This Report by calling BigMarketResearch.com at +1-971-202-1575.. About FISH Probe. FISH probe is a molecular diagnostic technique that utilizes labeled DNA probes to either detect or confirm gene or abnormalities in the chromosome. Analysts forecast the global fish probe market to grow at a CAGR of 7.34% during the period 2016-2020.. Covered in this report. The report covers the present scenario and the growth prospects of the global fish probe market for 2016-2020. To calculate the market size, we use the revenue generated from the sale of FISH probes.. Get sample copy of report @ https://goo.gl/CQ1yEO. The market is divided into the following segments based on geography:. • Americas. • APAC. • Europe. Global Fish Probe Market 2016-2020, has been prepared based on an in-depth market analysis with inputs from industry experts. The report covers the market landscape and its growth prospects over the coming years. The report also ...
Headline: Bitcoin & Blockchain Searches Exceed Trump! Blockchain Stocks Are Next!. The FISH Probe Industry report covers the present scenario and the growth prospects of the FISH Probe Market for 2016-2020. FISH Probe Market report focuses on the major drivers and restraints for the key players. To calculate the market size, the report considers both the direct revenue and the indirect revenue of the vendors. The global fish probe market to grow at a CAGR of 7.34% during the period 2016-2020.. FISH probe is a molecular diagnostic technique that utilizes labeled DNA probes to either detect or confirm gene or abnormalities in the chromosome.. Browse more detail information about FISH Probe Market Report at: http://www.absolutereports.com/global-fish-probe-market-2016-2020-10351017. The report covers the market landscape and its growth prospects over the coming years. The report also includes a discussion of the key vendors operating in this market.. Key Vendors in FISH Probe Market:. • Agilent ...
Fluorescence in situ hybridization (FISH) is a valuable cytogenetic technique for the detection and localization of specific DNA or RNA sequences on chromosomes. Typically, this technique can be used to define spatiotemporal expression patterns of target genes. Using fluorescent DNA probes with sequences complementary to the location of the chromosome of interest, gene expression patterns can be easily detected by fluorescence microscopy and further quantitatively analyzed. In addition, improved FISH analysis also allows simultaneous observation of multiple genes by labeling different sequence fragments with different fluorophores.. In plant systems, FISH analysis has been used in a variety of crops, including wheat, rye, cucumber and melon. Based on the information on Lifeasibles official website, Lifeasibles services cover every step of the FISH assay in the plant system:. • Probe designing and construction: aside from commercially available probes, Lifeasible also provides customized ...
Order Gene Specific Fluorescence In Situ Hybridization (FISH) Probes that have been designed and optimized for the gene of your specific research.
Survival rates for lung cancer are low because patients have disseminated disease at diagnosis; therefore tests for early diagnosis are highly desirable. This pilot study investigated occurrence of chromosomal aneusomy in sputum from a 33 case-control cohort matched on age, gender, and date of sample collection. Subjects had chronic obstructive pulmonary disease and , or = 30 pack-years of tobacco use, and aneusomy was tested using a multi-target DNA FISH assay (LAVysion, Abbott/Vysis). In specimens collected within 12 months of lung cancer diagnosis, abnormality was more frequent among the 18 cases (41%) than the 17 controls (6%; P = 0.04). Aneusomy had no significant association with cytologic atypia, which might indicate that molecular and morphological changes could be independent markers of tumorigenesis. Combining both tests, abnormality was found in 83% of the cases and 20% of the controls (P = 0.0004) suggesting that FISH may improve the sensitivity of cytologic atypia as a predictor of ...
Human chromosome-specific DNA libraries: Construction and availability. Bio-Technology 4: 537-552, 1986. Fuscoe JC, et al. Construction of fifteen human chromosome-specific DNA libraries from flow-purified chromosomes. Cytogenet. Cell Genet. 43: 79-86, 1986. PubMed: 3780319 Perlman J, Fuscoe JC. Molecular characterization of the purity of seven human chromosome-specific DNA libraries. Cytogenet. Cell Genet. 43: 87-96, 1986. PubMed: 3780320 Deaven LL, et al. Construction of human chromosome-specific DNA libraries from flow sorted chromosomes. Cold Spring Harbor Symp. Quant. Biol. 51: 159-167, 1986. PubMed: 3472712 Fuscoe JC. Human chromosome-specific DNA libraries: use of an oligodeoxynucleotide probe to detect non-recombinants. Gene 52: 291-296, 1987. PubMed: 3609744 Marvin Van Dilla, personal communication ...
hybridization (FISH) is a powerful molecular cytogenetic technique which allows rapid detection of aneuploidies on interphase cells and metaphase spreads. The aim of the present study was to evaluate FISH as a tool in prenatal diagnosis of aneuploidies in high risk pregnancies in an Indian set up. Prenatal diagnosis was carried out in 88 high-risk pregnancies using FISH and cytogenetic analysis. Multicolour commercially available FISH probes specific for chromosomes 13, 18, 21, X and Y were used. Interphase FISH was done on uncultured cells from chorionic villus and amniotic fluid samples. FISH on metaphase spreads was done from cord blood samples. The results of FISH were in conformity with the results of cytogenetic analysis in all the normal and aneuploid cases except in one case of structural chromosomal abnormality. The hybridization efficiency of the 5 probes used for the detection of aneuploidies was 100%. Using these probes FISH assay yielded discrete differences in the signal profiles ...
The purpose of the present study was to determine the effect of a single oral dose of carbendazim (CARB) on the frequencies of numerical chromosome aberrations in sperm and on micronuclei in peripheral blood erythrocytes of rats. Dual colour FISH on
Purpose: This study aimed to search for predictors of epidermal growth factor receptor (EGFR) tyrosine kinase inhibitors (TKI) efficacy in previously treated patients with advanced squamous cell lung carcinoma in which EGFR mutations are very rare.. Experimental Design: EGFR gene copy numbers were assessed by FISH and evaluated as predictors of EGFR-TKI efficacy in 71 patients with advanced squamous cell lung cancer who received gefitinib or erlotinib as a second-line or higher therapy. The tumors were classified into EGFR/FISH-positive (high polysomy/gene amplification) and EGFR/FISH-negative (other) groups.. Results: EGFR/FISH was positive in 19 (26.7%) patients. Only EGFR/FISH positive status was correlated with the EGFR-TKIs response (EGFR/FISH+ vs. EGFR/FISH−, 26.3% vs. 2.0%; P = 0.005). In a multivariate analysis, the risk of progression was lower in EGFR/FISH-positive patients (HR of EGFR/FISH+ vs. EGFR/FISH−, 0.57; P = 0.057) or patients experiencing grade 2 or more rash (HR for rash ...
Patient management based on HER2 status in breast carcinoma is an archetypical example of personalized medicine but remains hampered by equivocal testing and intratumoral heterogeneity. We developed a fully automated, quantitative, bright-field in situ hybridization technique (RNAscope), applied it to quantify single-cell HER2 mRNA levels in 132 invasive breast carcinomas, and compared the results with those by real-time quantitative PCR (qPCR) and Food and Drug Administration-approved methods, including fluorescence in situ hybridization (FISH), IHC, chromogenic in situ hybridization, and dual in situ hybridization. Both RNAscope and qPCR were 97.3% concordant with FISH in cases in which FISH results were unequivocal. RNAscope was superior to qPCR in cases with intratumoral heterogeneity or equivocal FISH results. This novel assay may enable ultimate HER2 status resolution as a reflex test for current testing algorithms. Quantitative in situ RNA measurement at the single-cell level may be ...
TAMGeS genotypes all the possible SNPs by performing three SBE reactions (P 1 , P 2 and U) with different combinations of labelled ddNTPs, hybridized on three identical arrays. Other dual-colour approaches request either a distinct array for each of the possible base changes or the use of one labelled allele-specific probe for each SNP [21, 23, 27].. Most of the existing methods, based both on tetra- and dual-colour approaches, do not succeed in genotyping all the SNPs with equal efficiency; SNPs with a too high data loss are simply discarded from the analysis. If many SNPs are analysed, as in large scale studies or wide genome scans, such a loss of data does not usually compromise the overall informative power of the study. On the contrary, in the context of association studies of candidate genes and SNPs, retrieving maximum information is essential. Our approach guarantees acquisition of reliable data also in those cases where SNP genotyping proves to be difficult. Indeed, with the three-array ...
Results. Comparison of sample recoveries for chromosome 3 fluorescence in-situ hybridization assay and mapping array analysis. Of the 59 patients who underwent FNAB, FISH results were obtained in 38 (64%) of the cases. Parallel, pooled aspirates (range; 2-4) from each patient were processed for simultaneous isolation of DNA and RNA, and the nucleic acid recoveries were determined. Where DNA recoveries exceeded 350 ng, samples were determined to be adequate for mapping array analysis. Of the 59 patients who underwent FNAB, 49 (83%) of the cases yielded adequate DNA, ranging 380-3040 ng. Six of these 49 failed to generate adequate probe for microarray due to melanin coprecipitation. Mapping array data were successfully obtained in the remaining 43 cases (73%) of the total cases. Mapping arrays not only provided data in all 38 cases where FISH data were obtained, but also provided data in five patients in whom FISH data were not obtained.. Comparison of findings of chromosome 3 fluorescence in-situ ...
10p Subtelomere(Texas Red) FISH Probe FE0092-20uL Labeled FISH probes for identification of subtelomere aberrations using Fluorescent In Situ Hybridization Tech
Principal Investigator:Taniwaki Masafumi, Project Period (FY):2016-04-01 - 2019-03-31, Research Category:Grant-in-Aid for Scientific Research (C), Section:一般, Research Field:Hematology
We have developed a procedure for efficient in situ hybridization of bacterial recombinants created with various types of large-insert cloning vectors. Minimal quantities of crude DNA are amplified and labeled during the degenerate-oligonucleotide-primed polymerase chain reaction. The resulting probes generate high-intensity fluorescent hybridization signals on metaphase chromosomes and on interphase nuclei.
LandMark™ broad range prestained protein marker(dual color) is a dual color prestained size marker. It is a mixture of 10 recombinant purified and prestained polypeptides whose molecular weights are well-adjusted ranging from 7,000 to 240,000 Da. It is provided preblended in a ready-to-use formula and no reconstitution or further dilution is necessary before use. A blue and pink chromophore is covalently bound to proteins, and 10 prestained proteins are visible during electrophoresis or electrophoretic transfer from the gel to a membrane. The 25 KDa and 70 KDa proteins labeled with orange chromophore offer easy identification and serve as a landmark. Because coupling of chromophore to the proteins affects their apparent molecular weights in SDS-PAGE, unstained protein standard should be used for accuracy ,95%.. ...
An increasing body of evidence indicates that the spatial positioning of genes in the interphase nucleus is highly relevant for their function (Lanctot et al, 2007; Meaburn & Misteli, 2007; Misteli, 2007). Fluorescence in situ hybridization (FISH) is a powerful technique to map gene loci in the interphase nucleus. Depending on protocol FISH can either detect DNA or RNA. Both methods have limitations. DNA FISH only detects the physical location of a gene, but can not detect gene activity. RNA FISH, on the other hand, detects transcripts, but might miss a significant number of alleles, since not all alleles of a gene are necessarily transcribed simultaneously. The most efficient way to map gene loci and their activity is sequential RNA and DNA FISH. This is an important technique to uncover how gene positioning is linked to activity.. Simultaneous detection of RNA and DNA for a gene locus is non-trivial. Procedures during DNA FISH particularly denaturation of cellular DNA, can cause significant ...
Sharpen your knives and come to attention because class is in session! Join Mike Cruz, manager of Greenpoint Fish & Lobster Wholesale, as he details the best methods for cleaning and preparing just about every fish you could encounter in the kitchen. Not every fillet is made the same and learning the proper technique can elevate your seafood game to the next level. So, if youre ready to learn how to fillet every fish, Mike has you covered and then some.
Labeled FISH probes for identification of subtelomere aberrations using Fluorescent In Situ Hybridization Technique. (Technology) (FE0030) - Products - Abnova
Labeled FISH probes for identification of subtelomere aberrations using Fluorescent In Situ Hybridization Technique. (Technology) (FE0119) - Products - Abnova
The purpose was to evaluate and compare 5 different HER2 genetic assays with different characteristics that could affect the performance to analyze the human epidermal growth factor 2 (HER2) gene copy number under low and high throughput conditions. The study included 108 tissue samples from breast cancer patients with HER2 immunohistochemistry (IHC) results scored as 0/1+, 2+, and 3+. HER2 genetic status was analysed using chromogenic in situ hybridization (CISH) and fluorescence in situ hybridization (FISH). Scoring results were documented through digital image analysis. The cancer region of interest was identified from a serial H&E stained slide following tissue cores were transferred to a tissue microarrays (TMA). When using TMA in a routine flow, all patients will be tested for HER2 status with IHC followed by CISH or FISH, thereby providing individual HER2 results. In conclusion, our results show that the differences between the HER2 genetic assays do not have an effect on the analytic ...
Easy and accurate HER2 testing is essential when considering the prognostic and predictive significance of HER2 in breast cancer. The use of a fully automated, quantitative FISH assay would be helpful to detect HER2 amplification in breast cancer tissue specimens with reduced inter-laboratory variab …
Empire Genomics RNA5-8S3 FISH probe is used to detect translocations of the RNA5-8S3 gene and can be labeled in one of five colors, using standard nick translation protocols. Each probe is sold in 20 test kits (~20 slides - 22x22 mm area) and includes hybridization buffer. Order 5 or more of the RNA5-8S3 FISH probe and save 10%!
The FN1 5 FISH Probe is designed to detect the human FN1 gene 5-region located on chromosome band 2q35. FN1 is also known as CIG, ED-B, FINC, FN, FNZ, GFND,GFND2, LETS or MSF. The FN1 5 FISH probe is labeled with CytoGreen. CytoGreen is a fluorophore with an excitation peak at 495nm and emission peak at 518nm, givin
00290] Karyotype analysis. Karyotype analysis was performed by Multicolor FISH analysis (M-FISH). Subconfluent STAPS cells were arrested in metaphase by colcemid (final concentration 0.270 μg/ml) to the culture medium for 2.5 h at 37°C in 5% C02. Cells were washed with PBS, treated with trypsin/ethylenediaminetetraacetic acid (EDTA), resuspended into cell medium and centrifuged for 5 min at 1200 rpm. To the cell pellet in 3 ml of PBS, 7 ml of a prewarmed hypotonic 0.0375 M KC 1 solution was added. Cells were incubated for 20 min at 37°C. Cells were centrifuged for 5 min at 1200 rpm and the pellet was resuspended in 3-5 ml of 0.0375 M KC1 solution. The cells were fixed with methanol/acetic acid (3: 1; vol/vol) by gently pipetting. Fixation was performed four times prior to spreading the cells on glass slides. For the FISH procedure, mouse chromosome-specific painting probes were combinatorially labeled using seven different fluorochromes and hybridized as previously described (Jentsch et al, ...
00290] Karyotype analysis. Karyotype analysis was performed by Multicolor FISH analysis (M-FISH). Subconfluent STAPS cells were arrested in metaphase by colcemid (final concentration 0.270 μg/ml) to the culture medium for 2.5 h at 37°C in 5% C02. Cells were washed with PBS, treated with trypsin/ethylenediaminetetraacetic acid (EDTA), resuspended into cell medium and centrifuged for 5 min at 1200 rpm. To the cell pellet in 3 ml of PBS, 7 ml of a prewarmed hypotonic 0.0375 M KC 1 solution was added. Cells were incubated for 20 min at 37°C. Cells were centrifuged for 5 min at 1200 rpm and the pellet was resuspended in 3-5 ml of 0.0375 M KC1 solution. The cells were fixed with methanol/acetic acid (3: 1; vol/vol) by gently pipetting. Fixation was performed four times prior to spreading the cells on glass slides. For the FISH procedure, mouse chromosome-specific painting probes were combinatorially labeled using seven different fluorochromes and hybridized as previously described (Jentsch et al, ...
Abnova 16q Subtelomere (DEAC) FISH Probe 1 Set Life Sciences:Biochemicals and Reagents:Fluorescent in situ hybridization (FisH) Reagents
Custom FISH Probes - Creative Bioarray is pleased to announce the introduction of custom labeled Fluorescence in situ Hybridization (FISH) Probes for a range of molecular and cytogenetic applications. What sets us apart in custom labeled probe manufacturing is our ability to consistently offer high quality locus-specific probes that can be customized to meet the specific needs of researchers and clinicians alike.
Cytogenetic Abnormalities with Interphase FISH Method and Clinical Manifestation in Chronic Lymphocytic Leukemia Patients in North-East of ...
Silver-enhanced in-situ hybridization (SISH) is an emerging tool for the determination of the Her-2/neu amplification status in breast cancer. SISH is technically comparable to fluorescence in-situ hybridization (FISH) but does not require a fluorescence microscope for its interpretation. Although recent studies on histologic evaluations of SISH are promising, we aimed to evaluate its performance on 71 cytologic breast cancer specimens with the new combined Her-2/Chr17 probe. Her-2/neu status as routinely determined by FISH was available for all patients. We found SISH signals in cytologic cell blocks and smear specimens easy to evaluate in most cases. Small numbers of tumor cells and difficulties in identifying tumor cells in lymphocyte-rich backgrounds were limiting factors. Her-2/neu status, as determined by Her-2/Chr17 SISH, was basically identical to the results of the corresponding FISH. The discrepancies were mainly owing to the heterogeneity of Her-2/neu amplification in the tumor ...
TY - JOUR. T1 - The effect of nucleobase-specific fluorescence quenching on in situ hybridization with rRNA-targeted oligonucleotide probes. AU - Behrens, Sebastian. AU - Fuchs, Bernhard M.. AU - Amann, Rudolf. PY - 2004/9. Y1 - 2004/9. N2 - Oligonucleotide probes labeled with fluorescent dyes are used in a variety of in situ applications to detect specific DNA or RNA molecules. It has been described that probe fluorescence might be quenched upon hybridization in a sequence specific way. Here, a set of 17 oligonuleotides labeled with 6-carboxyfluorescein was used to examine the relevance of nucleotide specific quenching for fluorescence in situ hybridization (FISH) to whole fixed bacterial cells. Probes quenched upon hybridization to a guanine-rich region of purified RNA in solution were not quenched upon FISH. Among other factors the high protein concentration within cells may prevent quenching of probe fluorescence in situ.. AB - Oligonucleotide probes labeled with fluorescent dyes are used in ...
In S. bulbocastanum, two strong, one intermediate, and one weak FISH signal were detected on four somatic metaphase chromosomes, with all signals located near the centromeric regions (Figure 2, H-J). Hybridization of 2D8 to NOR on S. bulbocastanum chromosomes was also observed (data not shown) but the cross-hybridization signals were not as strong as those observed in USW1. FISH on S. bulbocastanum pachytene chromosomes also yielded four distinct signals (Figure 2L), indicating that the four loci do not pair with one another and are therefore hemizygous. The high resolution pachytene FISH signals confirmed the pericentromeric locations of the 2D8 repeat (Figure 2M). The pachytene regions associated with the FISH signals are brightly stained by DAPI and highly condensed as compared to the distal euchromatic regions.. The 2D8 repeat is homologous to the IGS sequence of potato rDNA: One 5.9-kb 2D8 subclone was completely sequenced. This 5862-bp sequence consists of two diverged monomers of similar ...
Fluorescent proteins that can be reversibly photoswitched between a fluorescent and a nonfluorescent state are important for innovative microscopy schemes, such as protein tracking, fluorescence resonance energy transfer imaging, sub-diffraction resolution microscopy and others. However, all available monomeric reversibly switchable fluorescent proteins (RSFPs) have similar properties and switching characteristics, thereby limiting their use. Here, we introduce two bright green fluorescent RSFPs, bsDronpa and Padron, generated by extensive mutagenesis of the RSFP Dronpa, with unique absorption and switching characteristics. Whereas bsDronpa features a broad absorption spectrum extending into the UV, Padron displays a switching behavior that is reversed to that of all green fluorescent RSFPs known to date. These two RSFPs enable live-cell fluorescence microscopy with multiple labels using a single detection color, because they can be distinguished by photoswitching. Furthermore, we demonstrate dual-color
The current reversibly switchable fluorescent proteins (RSFPs) can not be multiplexed. Jakobs and colleagues create two RSFPs with novel switching characteristics that can be used simultaneously in fluorescence microscopy experiments using only one detection color. Fluorescent proteins that can be reversibly photoswitched between a fluorescent and a nonfluorescent state are important for innovative microscopy schemes, such as protein tracking1, fluorescence resonance energy transfer imaging2, sub-diffraction resolution microscopy3,4,5,6,7,8,9 and others. However, all available monomeric reversibly switchable fluorescent proteins (RSFPs) have similar properties and switching characteristics10,11,12, thereby limiting their use. Here, we introduce two bright green fluorescent RSFPs, bsDronpa and Padron, generated by extensive mutagenesis of the RSFP Dronpa10, with unique absorption and switching characteristics. Whereas bsDronpa features a broad absorption spectrum extending into the UV, Padron displays a
Background: Various genetic technologies have been employed in the identification of genomic complexity and refinement of prognostic classification of clinically heterogeneous disease of chronic lymphocytic leukemia (CLL). Objective: The present study of interphase cytogenetics and conventional karyotyping was undertaken to perform comprehensive analysis of CLL genetics with an approach to refine early prognostication of disease. Material & Methods: Retrospective analysis by fluorescence in situ hybridization (FISH) was carried out on total 671 patients of CLL at diagnosis between 2008 and 2015. Conventional cytogenetics studies were performed in 50 of 671 patients using CPG Oligonucleotide + IL-2 and TPA (12-O-Tetradecanyl Phorbol 13-acetate) for stimulation of lymphocytes cultures. Results: Interphase cytogenetics could detect recurrent abnormalities such as del(13q14), +12, del(17p13), del(11q22), del(6q23) in 71% of cases. The incidence of del(13q) was higher in Rai stage 0, I, II (p = 0.0005);
Introduction: HER2 gene amplification or overexpression occurs in 15% to 25% of breast cancers and has implications for treatment and prognosis. The most commonly used methods for HER2 testing are fluorescence in situ hybridization (FISH) and immunohistochemistry. FISH is considered to be the reference standard and more accurately predicts response to trastuzumab, but is technically demanding, expensive, and requires specialized equipment. In situ hybridization is required to be eligible for adjuvant treatment with trastuzumab in Australia. Bright-field in situ hybridization is an alternative to FISH and uses a combination of in situ methodology and a peroxidase-mediated chromogenic substrate such as diaminobenzidine [chromogenic in situ hybridization (CISH)] or multimer technology coupled with enzyme metallography [silver-enhanced in situ hybridization (SISH)] to create a marker visible under bright-field microscopy. CISH was introduced into diagnostic testing in Australia in October 2006. SISH ...
We detected comparable levels of asynchronous replication at 22q11.2 in all tested individuals, that is, control individuals and carriers of either translocations or deletions involving the 22q11.2 region. Based on cases with distinguishable chromosomes 22 we show a non-random nature of the asynchronous replication. In all cases where the origin of the structurally abnormal chromosome 22 was known we detected an earlier replication of the paternal alleles. We hypothesise that a non-random asynchronous replication in this region represents a risk factor for the formation of the 22q11.2 deletion by increasing the probability of an initial mispairing of the parental alleles at the highly homologous low-copy repeats. These initial abnormal conformations may lead, later in meiosis, to an unequal meiotic crossover and thus to the 22q11.2 deletion.. The replication timing results presented here were performed on peripheral blood cells. Our hypothesis would imply that the non-random asynchronous ...
Microbiological diagnosis of sepsis relies primarily on blood culture data. This study compares four diagnostic methods, i.e. those developed by us: nested, multiplex, qPCR (qPCR) and FISH with commercial methods: SeptiFast (Roche) (SF) and BacT/ALERT® 3D blood culture system (bioMérieux). Blood samples were derived from adult patients with clinical symptoms of sepsis, according to SIRS criteria, hospitalized in the Intensive Care Unit. Using qPCR, FISH, SF, and culture, microbial presence was found in 71.8%, 29.6%, 25.3%, and 36.6% of samples, respectively. It was demonstrated that qPCR was significantly more likely to detect microorganisms than the remaining methods; qPCR confirmed the results obtained with the SF kit in all cases wherein bacteria were detected with simultaneous confirmation of Gram-typing. All data collected through the FISH method were corroborated by qPCR. The qPCR and FISH methods described in this study may constitute alternatives to blood culture and to the few existing
TY - JOUR. T1 - Chromosomal gains measured in cytology samples from women with abnormal cervical cancer screening results. AU - Luhn, Patricia. AU - Houldsworth, Jane. AU - Cahill, Lynnette. AU - Schiffman, Mark. AU - Castle, Philip E.. AU - Zuna, Rosemary E.. AU - Dunn, S. Terence. AU - Gold, Michael A.. AU - Walker, Joan. AU - Wentzensen, Nicolas. PY - 2013/9/1. Y1 - 2013/9/1. N2 - Objective Chromosomal gains at 3q26, 5p15 and 20q13 have been described in cervical precancer and cancer. We evaluated a novel fluorescence in situ hybridization (FISH) assay that detects gains at these three loci simultaneously as a possible biomarker for detecting cervical precancer. Methods Chromosomal copy numbers at 3q26, 5p15, 20q13 and the centromere of chromosome7 (cen7) in liquid-based cytology specimens from 168 women enrolled in the Biopsy Study were determined by FISH. The number of cells with ≥ 3 or ≥ 4 signals for a genomic locus was enumerated and diagnostic test performance measures were ...
Three-dimensional structured illumination microscopy (3D-SIM) has opened up new possibilities to study nuclear architecture at the ultrastructural level down to the ~100 nm range. We present first results and assess the potential using 3D-SIM in combination with 3D fluorescence in situ hybridization (3D-FISH) for the topographical analysis of defined nuclear targets. Our study also deals with the concern that artifacts produced by FISH may counteract the gain in resolution. We address the topography of DAPI-stained DNA in nuclei before and after 3D-FISH, nuclear pores and the lamina, chromosome territories, chromatin domains, and individual gene loci. We also look at the replication patterns of chromocenters and the topographical relationship of Xist-RNA within the inactive X-territory. These examples demonstrate that an appropriately adapted 3D-FISH/3D-SIM approach preserves key characteristics of the nuclear ultrastructure and that the gain in information obtained by 3D-SIM yields new insights into
TY - JOUR. T1 - Detection of p16, RB, CDK4, and p53 gene deletion and amplification by fluorescence in situ hybridization in 96 gliomas. AU - Perry, Arie. AU - Anderl, Kari. AU - Borell, Tom J.. AU - Kimmel, David W.. AU - Wang, Chiao H.. AU - OFallon, Judith R.. AU - Feuerstein, Burt G.. AU - Scheithauer, Bernd W.. AU - Jenkins, Robert Brian. PY - 1999. Y1 - 1999. N2 - Inactivation of the p53 gene is a common early event of astrocytoma tumorigenesis. Alternatively, since the p16, retinoblastoma (RB), and CDK4 genes have been implicated in malignant progression, detection of losses or amplifications of these genes in gliomas could be diagnostically, prognostically, and therapeutically important. We obtained smear preparations from 96 diffuse gliomas and 10 nonneoplastic specimens. Dual-color fluorescence in situ hybridizations using paired probes for CEN9/p16, CEN8/RB, CEN17/p53, and CEN12/CDK4 were performed and revealed expected frequencies of abnormalities, except for p53 losses, which were ...
Small RNA Detection by in Situ Hybridization Methods. . Biblioteca virtual para leer y descargar libros, documentos, trabajos y tesis universitarias en PDF. Material universiario, documentación y tareas realizadas por universitarios en nuestra biblioteca. Para descargar gratis y para leer online.
Hardy, R R.; Hayakawa, K; Haaijman, J; and Herzenberg, L A., B lymphocyte subpopulations identifiable by two color fluorescence analysis. Abstr. (1982). Subject Strain Bibliography 1982. 1833 ...
A molecular cytogenetic map of sorghum chromosome 1: Fluorescence in situ hybridization analysis with mapped bacterial artificial chromosomes - Texas A&M University (TAMU) Scholar profile, educations, publications, research, recent courses, and student works
Segmental duplicons (SDs) predispose to an increased frequency of chromosomal rearrangements. These rearrangements can cause a diverse range of phenotypes due to haploinsufficiency, in cis positional effects or gene interruption. Genomic microarray analysis has revealed gene dosage changes adjacent to duplicons, but the high degree of similarity between duplicon sequences has confounded unequivocal assignment of chromosome breakpoints within these intervals. In this study, we localize rearrangements within duplicon-enriched regions of Angelman/Prader-Willi (AS/PWS) syndrome chromosomal deletions with fluorescence in situ hybridization (FISH). Breakage intervals in AS deletions were localized recursively with short, coordinate-defined, single copy (SC) and low copy (LC) genomic FISH probes. These probes were initially coincident with duplicons and regions of previously reported breakage in AS/PWS. Subsequently, probes developed from adjacent genomic intervals more precisely delineated deletion breakage
FISH-positive criteria have been used according to the corresponding genes. FGFR1 high-level amplification is defined as copy number ≥ 9 or (1) an FGFR1/CEP8 ratio of ≥ 2.0, (2) the average number of FGFR1 signals per tumor cell nucleus ≥ 6, and (3) the percentage of tumor cells containing ≥ 15 FGFR1 signals or large clusters ≥ 10% [74,87]. According to previously published criteria, the EGFR gene copy number was classified into six FISH strata: disomy (two or fewer copies in more than 90% of cells), low trisomy (two or fewer copies in 40% or more of the cells, three copies in 10%-40% of cells, and four or more copies in less than 10% of cells), high trisomy (two or fewer copies in ≥ 40% of cells, three copies in ≥ 40% of cells, and four or more copies in less than 10% of cells), low polysomy (four or more copies in 10%-40% of cells), high polysomy (four or more copies in 40% of the cells or more), and gene amplification (defined by the presence of tight EGFR clusters and a ratio ...
A 10-year-old girl with Ullrich-Turner syndrome was found to have the novel karyotype 45,X/46,X,r(X)(p11q11)/46,X,dic(X)(p11). Fluorescence in situ hybridization (FISH) with the alpha satellite X centromere probe established the origin of the small ring chromosome. Scanning a large number of cells by interphase FISH showed that the dicentric (X) was the least prevalent cell line. The common breakpoint of Xp11 suggests a sequence of errors as the mechanism whereby these 3 distinct cell lines have arisen.
TY - JOUR. T1 - High rate of neoplastic cells with genetic abnormalities in proliferation centers of chronic lymphocytic leukemia. AU - Balogh, Zsófia. AU - Reiniger, Lilla. AU - Rajnai, Hajnalka. AU - Csomor, Judit. AU - Szepesi, Ágota. AU - Balogh, Anikó. AU - Deák, Linda. AU - Gagyi, Éva. AU - Bödör, Csaba. AU - Matolcsy, A.. PY - 2011/6. Y1 - 2011/6. N2 - In lymph nodes, chronic lymphocytic leukemia (CLL) cells (prolymphocytes and paraimmunoblasts) form proliferation centers (PCs), which are also known as pseudofollicles. To reveal whether PCs play a role in the accumulation of genetic alterations in CLL, we compared deletion at 11q22.3, 13q14.3, and 17p13.1 loci and trisomy 12 by the fluorescence in situ hybridization (FISH) technique in PCs versus surrounding small lymphocytes (SLs) in 12 formalin-fixed paraffin-embedded (FFPE) lymph nodes. The FFPE sections were stained with methylene blue and PCs were marked by laser beam. Subsequent FISH analysis was performed, relocalizing the ...
Rosetta Genomics Ltd. operates as a genomic diagnostics company worldwide. The company ?s microRNA technologies based diagnostic tests include RosettaGX Cancer Origin for the identification of the primary site of metastatic cancer; mi-KIDNEY, a kidney tumor classification test for pathology samples; RosettaGX Reveal for the diagnosis of indeterminate thyroid fine-needle aspirate samples; and mi-LUNG diagnostic tests. It also provides UroVysion, a urine-based Fluorescence In Situ Hybridization (FISH) assay that is intended for use in conjunction with and not in lieu of current standard diagnostic procedures, as an aid for initial diagnosis of bladder carcinoma in patients with hematuria and subsequent monitoring for tumor recurrence in patients previously diagnosed with bladder cancer; and ERG/PTEN, which are FISH-based prognostic tests in prostate cancer. In addition, the company offers ALK/ROS1 that are FISH-based predictive tests indicated for patients who are diagnosed with late stage lung ...
XMP are chromosome-specific and comprise mouse whole chromosome painting probes which are directly labeled with an emitting fluorochrome.
In situ hybridization allows us to learn more about the geographical location of, for example, the messenger RNA (mRNA) in a cell or tissue. It can also tell us where a gene is located on a chromosome. Obviously, a detection system must be built into the technique to allow the cytochemist to visualize and map the geography of these molecules in the cells in question.. When in situ hybridization was first introduced, it was applied to isolated cell nuclei to detect specific DNA sequences. Early users applied the techniques to isolated chromosomal preparations in order to map the location of genes in those chromosomes. The technique has also been used to detect viral DNA in an infected cell. In situ hybridization of RNA has also been used to show that RNA synthesis (transcription) occurs in the nucleus, while protein synthesis (translation) occurs in the cytoplasm.. ...
Hello Debbie, As usual, I can not offer an unbiased opinion. However, InnoGenex does carry many FISH detection systems and two Her-2 probes[one DNA probe & one mRNA probe]. The systems are sensitive, fast and very consistent ; the ISH kits are currently used in diagnostic assays at major hospitals/research institutions(want a reference?). I have enclosed a manual from one of the FISH kits. If you would like the data sheets for the two Her-2 probes, let me know. Regards, Matthew Ogdie InnoGenex (925)543-1414 http://www.innogenex.com -----Original Message----- From: Jennings-Siena, Debbie [mailto:[email protected]] Sent: Tuesday, June 06, 2000 10:21 AM To: [email protected] Subject: Her 2 by FISH If your lab is doing Her 2 by Fluorescent In-situ Hybridization techniques (FISH) could you please tell me which manufacturers kit that you are using and how you like it? My pathologist would like to know whose kit has the highest reputation, I would also like to know about ...
Despite significant advances in molecular genetic approaches, fluorescence in situ hybridization (FISH) remains the gold standard for the diagnostic evaluation of genomic aberrations in patients with chronic lymphocytic leukemia (CLL). Efforts to improve the diagnostic utility of molecular cytogenetic testing have led to the expansion of the traditional 4-probe CLL FISH panel. Not only do these efforts increase the cost of testing, they remain hindered by the inherent limitations of FISH studies - namely the inability to evaluate genomic changes outside of the targeted loci. While array-based profiling and next generation sequencing (NGS) have critically expanded our understanding of the molecular pathogenesis of CLL, these methodologies are not routinely used by diagnostic laboratories to evaluate copy number changes or the mutational profile of this disease. Mitogenic stimulation of CLL specimens with CpG-oligonucleotide (CpG-ODN) has been identified as a reliable and reproducible means of ...
Create ELEGANTLY GLITTERY, GRADIENT COLORED eye-shadows in a second with our Dual-color Eye-shadow! Simply swipe once to create dual colored eye-shadow without professional technique. No fussy layering and blending! This eye-shadow is highly pigmented with exquisite glitters and smooth texture. Buildable colors - You can increase intensity by brushing repeatedly. One swipe for daily/ casual make-up, and more swipes for party & sexy makeup! ☑ FEATURES: ✅ One-swipe Design Include dual colors for instant gradient shading! Easy to create gorgeous, smooth gradient colors for a delicate, natural eye makeup. No fussy layering and blending. ✅ Elegant & Shimmery Highly pigmented colors with exquisite glitters and smooth texture. ✅ Long-lasting Lasts up to 15 hours with proper makeup preparation. Waterproof & Smudge-proof ✅ Easy to Apply & Remove Shorten your makeup routine as you only swipe once to complete your eye makeup. Use makeup remover to remove it easily without leaving any glitter/
We use cookies to ensure that we give you the best experience on our website. If you click Continue well assume that you are happy to receive all cookies and you wont see this message again. Click Find out more for information on how to change your cookie settings ...
Gentaur molecular products has all kinds of products like :search , Cell Sciences \ Human IL2_IL10 Dual Color ELISPOT Kit w_o plates \ 874.050.010 for more molecular products just contact us
Gentaur molecular products has all kinds of products like :search , Cell Sciences \ Human IL2_IL10 Dual Color ELISPOT Kit w_plates \ 874.050.010P for more molecular products just contact us
Fig. 2. Standard diagnostic techniques are not optimal for the routine detection of ALK-rearranged NSCLC. Representative ALK-rearranged (A-F) and ALK germ-line (G-I) tumors analyzed by FISH using probes flanking the ALK gene (A, D, and G), standard immunohistochemical staining for ALK protein (B, E, and H), and tyramide-amplified immunohistochemical staining for ALK protein (C, F, and I). Red arrows, split FISH probes characteristic of an ALK rearrangement; yellow arrow, nonsplit FISH probes characteristic of ALK germ-line. ...
An ultrasonic probe having an electronic radial transducer is disclosed. In the ultrasonic probe, a plurality of piezoelectric elements having electrodes on both surfaces thereof are arranged and juxtaposed on a circular backing member. A doughnut shaped substrate is provided on an end surface of the piezoelectric elements of the electronic radial transducer and having connection points arranged in a signal pattern conductive to one each electrode of the piezoelectric elements. Additionally, at least one overlapping substrate having connection points arranged in accordance with the respective connection points of the signal pattern of the doughnut shaped substrate.
An analysis of the chromosomal aberrations and DNA ploidy in the interphase nuclei of seven human osteosacomas was preformed by double-target fluorescence in situ hybridization (FISH) and DNA cytofluorometry. The FISH study of the numerical aberrations in chromosomes 1 and 17 or the structural aberrations in chromosome arm 1p or 17p was carried out by using four locus specific DNA markers, with one pair consisting of 1q12 and 1p36 and the other pair consisting of the 17 cemtromere and 17p13.3. There was no significant differences in the percentage of deletions in chromosome 1 and 17 between osteosarcomas and normal tissues ...
Ovrednotili so zmogljivost štiribarvnega ERG/PTEN QD ISH testa pri optimalni pred obdelavi vzorcev. Signale so prešteli pri 389 vzorcih, ki so jih pridobili iz desetih tkiv prostate. Izkazalo se je, da je bilo 91% obarvanj sprejemljivih tako za ERG3p ter ERG5p, kot tudi za PTEN ter CEN10. Ne sprejemljivih je bilo samo 36 od 386 preparatov, od katerih je pri 28 prišlo do visokega ozadja pri QD655, pri osemih pa so bili signali prešibki oziroma jih sploh ni bilo. Med preparati s sprejemljivim obarvanjem je bilo 280 takih, ki so jih pridobili iz osem vzorcev benignega tkiva prostate in 70 takih, ki so bili pridobljeni iz dveh vzorcev tkiv z rakom prostate. Naključno so izbrali 19 preparatov pripravljenih iz dveh benignih tkiv in dveh tkiv z rakom prostate, katerim so prešteli signale za ERG3p, ERG5p, PTEN in CEN10 na jedro celice v treh različnih dneh. Tako pridobljeni podatki so pokazali, da so rezultati obarvanja konstanti skozi vse dni štetja. Pri preparatih iz vzorca benignega tkiva se ...
Sigma-Aldrich offers abstracts and full-text articles by [Darcy A Kerr, Kshitij S Arora, Krishnan K Mahadevan, Jason L Hornick, Jeffrey F Krane, Miguel N Rivera, David T Ting, Vikram Deshpande, William C Faquin].
Fluorescent In situ Hybridization, is fast and specific technique which can be used to detect alteration in numerical and structural chromosomal anomalies. With technological advancement multicolour FISH (m-FISH or SKY FISH) can be carried out and single probe (for just one chromosome) can also be carried out as per the need of case. It can also be employed in small deletion, duplication, translocation and chromatin fusion attributed disorders. Certain key features that make FISH a valuable tool is turn around time, which is less than 24 hours. Another advantage of FISH based analysis is it can be carried out in interphase nuclei, ruling out the need of culture and thus playing a crucial role in non dividing cells or dead cells where chromosomal analysis is not possible. Few of FISH based test are enlisted below and situations where those tests can be utilized.. ...
Weve found through our studies that fish do have a memory. … Its the same way for the fishs buddies that observed that fish being caught, too. When they see the lure come past, they are going to remember and they are going to avoid it. The same holds true for lakes that are exposed to heavy fishing pressure ...
Ocean fish decomposed with microbes - an ideal base for DIY fertilisers, due to the potential for amino acid chelation. ACO organic certified.
The principal findings of this study are the following: (a) the results of FISH/CISH analysis done on small-sized specimens represent a successful method for establishing the EGFR/HER2 gene content in NSCLC; (b) as reported in the literature, gain of EGFR and HER2 genes is more frequently a consequence of Chr7 and Chr17 polysomy, respectively, rather than gene amplification; and (c) concurrent polysomy and, less specifically, concurrent trisomy of EGFR and HER2 may be considered as positive markers for selecting NSCLC patients eligible for TKI treatment.. The first aim of our study was to validate the EGFR/HER2 gene study by FISH or CISH in very small tissue samples of lung cancers. In our series, in 68% of cases, the first diagnosis of NSCLC was obtained from small biopsies or cytologic samples. This limited the possibility of characterizing the histotype of tumors in 7% of cases. Several studies have reported successful use of FISH and CISH in alcohol-fixed fine-needle aspiration cytology or ...
The MYH11/CCP16 FISH Probe Kit is designed to detect the human MYH11 gene located on chromosome band 16p13.11 along with the number of chromosome 16 copies per
Hello Everyone, I was hoping to get some insight on the results of a FISH test. a quick background - gross hematuria 3 months ago, MRI negative showed cyst on...
PURPOSE: HER2 is a clinically important tumor marker in breast cancer; however, there is controversy regarding which method reliably measures HER2 status. We compared three HER2 laboratory methods: immunohistochemistry (IHC), fluorescence in situ hybridization (FISH) and polymerase chain reaction (PCR), to predict disease-free survival (DFS) and overall survival (OS) after adjuvant doxorubicin-based therapy in node-positive breast cancer patients. METHODS: This is a Cancer and Leukemia Group B (CALGB) study, using 524 tumor blocks collected from breast cancer patients registered to clinical trial CALGB 8541. IHC employed CB11 and AO-11-854 monoclonal antibodies; FISH used PathVysion HER2 DNA Probe kit; PCR utilized differential PCR (D-PCR) methodology. RESULTS: Cases HER2 positive by IHC, FISH and D-PCR were 24%, 17%, and 18%, respectively. FISH and IHC were clearly related (kappa = 64.8%). All three methods demonstrated a similar relationship for DFS and OS. By any method, for patients with HER2
Huge collection of designer karen millen occasion dresses are on sale up to 85% off,buy Acetate Karen Millen Floral Stretch Midi Dress - Multicolour Occasion Dresses Multicolour right now with free delivery.
Please Note: ProSci now has over 700 different lots available for a wide range of tissues. Many of these lots are not listed online. If you dont see what you are looking for please call us.Formalin-fixed, paraffin-embedded tissue is useful for a variety of applications including the targeting of proteins, RNA, and DNA by means of Immunohistochemistry (IHC), in-situ hybridization (ISH) and fluorescence in-situ hybridization (FISH), respectively. Some tissue types may require extra attention as they may contain high levels of endogenous biotin, peroxidase, or autofluorescence ...
Single-molecule RNA FISH was conducted as described previously (Lee et al., 2013a). Cells grown overnight in AFM were fixed with formaldehyde (final concentration 3.7% vol/vol) at 30°C for 1 h on the shaker and washed twice with ice-cold buffer B (1.2 M sorbitol and 0.1 M potassium phosphate, pH 7.5). The fixed cells were resuspended in 1 ml spheroplasting buffer (10 ml buffer B and 2 mM vanadyl ribonucleoside complex) and transferred to a new RNase-free microcentrifuge tube. 1.5 mg Zymolase (MP Biomedicals) was added to the cells and incubated at 37°C for 35 min for wild-type and 10 min for whi3Δ cells, and cells were washed twice with buffer B. Cells were resuspended in 1 ml RNase-free 70% overnight at 4°C. RNA FISH probes (Biosearch Technologies) were resuspended in 20 µl TE buffer (10 mM TrisCl and 1 mM EDTA, pH 8.0). Then probes were diluted 1:10 from initial probe stock (250 µM in TE buffer). On the next day, cells were washed with wash buffer (20× SSC, 10% vol/vol deionized ...
Visualize Gene Expression & Genetic Variations in Tissues:. Applications of RNAscope® and BaseScopeTM ISH technology. The nervous system consists of numerous specialized cell types that remain to fully cataloged and characterized at the molecular level. Due to the high degree of structural and functional heterogeneity and the intricate spatial organization of these cells, it is of special importance to analyze gene expression in the presence of full morphological and spatial contexts. Due to the lack of specific antibody reagents, especially for lncRNAs, G-protein coupled receptors (GPCRs), and ion channels, mapping of specific transcripts by in situ hybridization offers an excellent alternative approach. The RNAscope® assay provides a powerful method to detect gene expression within the spatial and morphological tissue context. BaseScopeTM is a novel in situ hybridization technology that allows visualization of splice junctions between adjacent exons and/or retained introns in highly specific ...
TY - JOUR. T1 - Assignment of human PLD2 to chromosome band 17p13.1 by fluorescence in situ hybridization. AU - Park, S. H.. AU - Ryu, S. H.. AU - Suh, P. G.. AU - Kim, H.. PY - 1998. Y1 - 1998. UR - http://www.scopus.com/inward/record.url?scp=0032408146&partnerID=8YFLogxK. UR - http://www.scopus.com/inward/citedby.url?scp=0032408146&partnerID=8YFLogxK. M3 - Article. C2 - 9858823. AN - SCOPUS:0032408146. VL - 82. JO - Cytogenetic and Genome Research. JF - Cytogenetic and Genome Research. SN - 1424-8581. IS - 3-4. ER - ...
RESULTS: Samples from 9 malformed fetuses were analyzed successfully by CGH. Numerical chromosome aberrations were detected in samples from cases 4, 8 and 9, and were verified by fluorochrome-exchanged CGH. Trisomy 21q was detected in case 4, del 2p24-pter and dup 12p13 was detected in case 8, and del 1p33-pter and del 22q11-12 were detected in case 9 ...
I am trying to set up doing in situ hybridization on paraformaldehyde fixed sections from mouse embryos using a 24mer oligonucleotide. I would appreciate tips on the best way to do this: end labelling or A-tailing. 3H or 35S as label. Also the best way to work out hybridization and washing conditions. All recipes gratefully received. Kathy Cheah HRMBDKC at HKUCC.BITNET ...
Fluorescent in situ hybridisation (FISH) FISH can be used to detect structural rearrangements, gene amplifications, translocations, microdeletions.
Fluorescence In Situ Hybridization maps out single copy or repetitive DNA sequences through localization labeling of specific ... It includes a series of techniques referred to as fluorescence in situ hybridization, or FISH, in which DNA probes are labeled ... O'Connor C (2008). "Fluorescence In Situ Hybridization (FISH)". Nature Education. 1 (1): 171. Martin EA, McFerran TA, eds. ( ... FISH chromosome in-situ hybridization allows the study cytogenetics in pre- and postnatal samples and is also widely used in ...
... in situ hybridization https://www.youtube.com/watch?v=1iRynlXIJw0 A detailed description of Fluorescence In Situ Hybridization ... Fluorescence in situ hybridization (FISH)is the most widely used riboprobe technique. A target sequence and a probe are ... O'Connor, Clare (2008). "Fluorescence In Situ Hybridization (FISH)". Nature Education. 1: 171. Lajtha, Abel (2007). Handbook of ... www.nature.com/scitable/topicpage/fluorescence-in-situ-hybridization-fish-327# Riboprobe In Vitro Transcription Systems ...
1 by fluorescence in situ hybridization". Genomics. 40 (1): 213-5. doi:10.1006/geno.1996.4540. PMID 9070952. Ladner RD, ...
... q23 by fluorescence in situ hybridization". Cytogenet Cell Genet. 68 (1-2): 122-124. doi:10.1159/000133905. PMID 7956350. ... to band 15q22 by fluorescence in situ hybridization". Cytogenet Cell Genet. 69 (1-2): 15-17. doi:10.1159/000133928. PMID ... to band 9p13 by fluorescence in situ hybridization". Cytogenet Cell Genet. 71 (1): 94-95. doi:10.1159/000134070. PMID 7606936. ... to band 19p13.1 by fluorescence in situ hybridization". Cytogenet Cell Genet. 72 (4): 294-296. doi:10.1159/000134206. PMID ...
... q13.2 by fluorescence in situ hybridization". Cytogenetics and Cell Genetics. 71 (2): 179-81. doi:10.1159/000134102. PMID ... this was done using fluorescence in situ hybridization. BLVRB encodes a protein that is a 206-residue monomeric enzyme. The ...
and Lansdorp, PM (2001) "Quantitative Fluorescence in-situ Hybridization." Current Protocols in Cell Biology (University of ... Quantitative Fluorescent in situ hybridization (Q-FISH) is a cytogenetic technique based on the traditional FISH methodology. ... Following hybridization, thousands of cells can be analyzed on a flow cytometer in a relatively short time. However, Flow-FISH ... A small volume of the hybridization mixture is placed onto a coverslip and then placed gently onto the microscope slide which ...
Detection is done by fluorescence in situ hybridization (FISH). Larger chromosomal deletion syndromes are detectable using ...
Shaffer LG (15 May 2001). "Diagnosis of Microdeletion Syndromes by Fluorescence in situ Hybridization (FISH)". Current ... Like other chromosomal microdeletions, 17q12 microdeletion syndrome is diagnosed via fluorescence in situ hybridization. ...
Wendeberg A (January 2010). "Fluorescence in situ hybridization for the identification of environmental microbes". Cold Spring ...
1998). "Regional mapping of the human MP70 (Cx50; connexin 50) gene by fluorescence in situ hybridization to 1q21.1". Mol. Vis ...
Finally, using fluorescence in situ hybridization (FISH), Kashuba et al. were able to map the RAB7A gene to 3q21 in 1997. RAB7a ...
3 by fluorescence in situ hybridization and radiation hybrid mapping". Genomics. 50 (1): 112-4. doi:10.1006/geno.1998.5227. ...
"An integrated microfluidic chip for chromosome enumeration using fluorescence in situ hybridization". Lab on a Chip. 8 (12): ... fluorescence resonance energy transfer) in optical signal indicates a reaction has occurred. Fluorescence-based detection has ... In situ microscopy assays with microfluidic cell cultures may help in this regard, but have inherently lower throughput due to ... Optical detection includes fluorescence-based techniques, chemiluminescence-based techniques, and surface plasmon resonance ( ...
alboglabra by fluorescence in situ hybridization" - Nature "Chromonema and chromomere" - Springerlink. ...
"Telomere Length Measurements Using Fluorescence In Situ Hybridization and Flow Cytometry". Cytometry, 4th Edition: New ...
Additionally, LNA has been incorporated in fluorescence in situ hybridization (FISH). FISH is a common technique used to ... Kubota, Kengo; Ohashi, Akiyoshi; Imachi, Hiroyuki; Harada, Hideki (August 2006). "Improved in situ hybridization efficiency ... Elayadi, Anissa N.; Braasch, Dwaine A.; Corey, David R. (August 2002). "Implications of High-Affinity Hybridization by Locked ... Conversely, LNA-incorporated probes demonstrated increased hybridization efficiency in both DNA and RNA. The improved ...
Kesara introduced the techniques of Fluorescence In Situ Hybridization (FISH) to Iceland. Firstly, the techniques were adopted ... Genomic in situ hybridization to identify alien chromosomes and chromosome segments in wheat. Theoretical and Applied Genetics ... called Genomic In Situ Hybridization (GISH). The method instantly proved applicable to detecting chromosome transfers in cereal ... revealed by in situ hybridization. Chromosome Research 9: 243-249. DOI: 10.1023/A:1016604705296 Víctor Lucía, M. Montserrat ...
... constitutive expression and localization by fluorescence in situ hybridization". Brain Res. Mol. Brain Res. 85 (1-2): 123-32. ... and localization by fluorescent in situ hybridization". J. Neurochem. 72 (3): 1170-8. doi:10.1046/j.1471-4159.1999.0721170.x. ...
Antón J, Llobet-Brossa E, Rodríguez-Valera F, Amann R (December 1999). "Fluorescence in situ hybridization analysis of the ... This is seen in cases such as the genus Haloarcula, which is estimated to make up less than 0.1% of the in situ community, but ... from these suggest that some of the most readily isolated and studied genera may not in fact be significant in the in situ ...
"Reassignment of MYCL1 to human chromosome 1p34.3 by fluorescence in situ hybridization". Cytogenet Cell Genet. 72 (2-3): 189- ... "Allelic deletions of Rb and L-myc in urine sediments from patients with bladder tumors or carcinoma in situ". Oncol. Rep. 9 (3 ...
"Localization of the human collagen gene COL7A1 to 3p21.3 by fluorescence in situ hybridization". Cytogenetics and Cell Genetics ...
CO-FISH, or strand-specific fluorescence in situ hybridization, facilitates strand-specific targeting of DNA with fluorescently ... "Chromosome orientation fluorescence in situ hybridization to study sister chromatid segregation in vivo". Nature Protocols. 5 ( ... detection by fluorescence and induction by mitomycin C". Proceedings of the National Academy of Sciences of the United States ... the daughter cells are synchronized at the G2 phase and individually separated by fluorescence-activated cell sorting (FACS). ...
Voelter-Mahlknecht S, Letzel S, Mahlknecht U (Apr 2006). "Fluorescence in situ hybridization and chromosomal organization of ...
"Assignment of human PLD2 to chromosome band 17p13.1 by fluorescence in situ hybridization". Cytogenetics and Cell Genetics. 82 ...
"Localization of human DLX8 to chromosome 17q21.3-q22 by fluorescence in situ hybridization". Mamm. Genome. 8 (4): 302-3. doi: ...
"The human HERC3 gene maps to chromosome 4q21 by fluorescence in situ hybridization". Cytogenet Cell Genet. 87 (3-4): 263-264. ...
"Assignment of the human GLI2 gene to 2q14 by fluorescence in situ hybridization". Genomics. 36 (1): 220-1. doi:10.1006/geno. ...
"The human HCLS1 gene maps to chromosome 3q13 by fluorescence in situ hybridization". Cytogenet Cell Genet. 72 (2-3): 175-6. doi ...
The radixin gene has been localized by fluorescence in situ hybridization to 11q23. A truncated version representing a ...
"Chromosomal mapping of the human histone gene H2AZ to 4q24 by fluorescence in situ hybridization". Genomics. 20 (2): 333-5. doi ...
Chromosomal assignment of human nuclear envelope protein genes LMNA, LMNB1, and LBR by fluorescence in situ hybridization. ...
Wikimedia Commons has media related to Fluorescence in situ hybridization.. *Fluorescent+in+Situ+Hybridization at the US ... Fluorescence in situ hybridization (FISH) is a molecular cytogenetic technique that uses fluorescent probes that bind to only ... Fluorescence in situ Hybridization Photos of bacteria. *Rational design of polynucleotide probe mixes to identify particular ... In-Solution Fluorescent In Situ Hybridization and Fluorescence-Activated Cell Sorting for Single Cell and Population Genome ...
... can also be conjugated to nucleoside triphosphates and incorporated into a probe enzymatically for in situ hybridisation. The ... The fluorescence that is created by the dye makes problem areas more visible and easily identified. A similar concept can be ... The fluorescence of this molecule is very intense; peak excitation occurs at 494 nm and peak emission at 521 nm. ... In cellular biology, the isothiocyanate derivative of fluorescein is often used to label and track cells in fluorescence ...
Fluorescence in situ hybridization. Clinical pathology. *Clinical chemistry. *Hematopathology. *Transfusion medicine. *Medical ...
... by fluorescence in situ hybridization". Cytogenet. Cell Genet. 57 (2-3): 109-11. PMID 1914517. doi:10.1159/000133124. CS1 ...
Fluorescence in situ hybridization. Clinical pathology. *Clinical chemistry. *Hematopathology. *Transfusion medicine. *Medical ... quantitative PCR does not require this, as the detection system uses fluorescence and probes to detect the DNA molecules as ... Electron microscopes and fluorescence microscopes are also used for observing microbes in greater detail for research.[28] ...
... fluorescent in situ hybridization, methylation-sensitive restriction enzymes, DNA adenine methyltransferase identification ( ... Fluorescence, Pigment & Radioactivity. High-throughput technique ("-omics"). DNA microarray. Mass spectrometry. Lab-on-a-chip. ...
Fluorescence in situ hybridization (FISH) involves fluorescent labeling of probes that bind to specific DNA sequences, used for ... Array comparative genomic hybridization is a new molecular technique that involves hybridization of an individual DNA sample to ... and comparative genomic hybridization tests. Some Clinical Geneticists are also board certified in Cytogenetics.. College (4 ... new molecular technologies such as array comparative genomic hybridization are now becoming widely used. Examples of chromosome ...
... by employing fluorescent in situ hybridization (FISH) and/or post-sequencing confirmation.[10] The bias of MDA against high %GC ... Fluorescence-activated cell sorting (FACS) is a widely used approach. Individual cells can also be collected by ...
... fluorescence in situ hybridization (FISH) and microsatellite analysis". Conservation Genetics. 6 (1): 141-145. doi:10.1007/ ... Interspecific hybridisation of black and white rhinoceros has also been confirmed.[9] ... Robinson, Terry J.; V. Trifonov; I. Espie; E.H. Harley (January 2005). "Interspecific hybridization in rhinoceroses: ...
Fluorescence in situ hybridization. Clinical pathology. *Clinical chemistry. *Hematopathology. *Transfusion medicine. *Medical ... A fluorescence microscope is then used to detect fluorescently labeled antibodies bound to internalized antigens within ...
... by fluorescence in situ hybridization, in Cytogenet. Cell Genet., vol. 57, 2-3, 1991, pp. 109-11, DOI:10.1159/000133124, PMID ...
Fluorescence in situ hybridization. Clinical pathology. *Clinical chemistry. *Hematopathology. *Transfusion medicine. *Medical ...
... p34.3 by fluorescence in situ hybridization.". Cytogenet. Cell Genet. 72 (2-3): 205-7. PMID 8978777. doi:10.1159/000134190.. ...
Chromosomal localization of the human histone H2A.X gene to 11q23.2-q23.3 by fluorescence in situ hybridization. . In: Hum. ...
Fluorescence in situ hybridization. Clinical pathology. *Clinical chemistry. *Hematopathology. *Transfusion medicine. *Medical ...
Fluorescence in situ hybridization. Clinical pathology. *Clinical chemistry. *Hematopathology. *Transfusion medicine. *Medical ... "Sorting Out Fluorescence Activated Cell Sorting". Retrieved 2017-11-09.. *^ Julius MH, Masuda T, Herzenberg LA (1972). " ... Java Fluorescence Spectrum Viewer (Becton, Dickinson and Company). *The History of the Cell Sorter Interviews from the ... The current record for a commercial instrument is ten lasers[6] and 30 fluorescence detectors.[7] Increasing the number of ...
Fluorescence in situ hybridisation has also been described, but has not been clinically validated, and it is not commercially ... 2011). "Rapid identification of Burkholderia pseudomallei and Burkholderia mallei by fluorescence in situ hybridization (FISH) ...
... to chromosome 7q34-q35 by two-color fluorescence in situ hybridization". Genomics. 16 (3): 771-773. PMID 8325653. doi:10.1006/ ...
... insight from the analysis of the food vacuole by means of fluorescence in situ hybridization". FEMS Microbiology Ecology 52 (3 ...
... and melanocortin-3 receptors to chromosomes 18p11.2 and 20q13.2-q13.3 by fluorescence in situ hybridization.". Genomics. 18 (1 ...
2002). DIG Application Manual for Nonradioactive in situ Hybridization (3rd ed.). Penzberg: Roche Diagnostics.. ... "Competitive homogeneous digoxigenin immunoassay based on fluorescence quenching by gold nanoparticles". Analytica Chimica Acta ... sensitive non-radioactive in situ hybridization probes to detect nucleic acids in plants, able to detect 1 µg of plasmid DNA.[6 ... Hauptmann G, Gerster T (August 1994). "Two-color whole-mount in situ hybridization to vertebrate and Drosophila embryos". ...
"Assignment of the human gene for KBF2/RBP-Jk to chromosome 9p12-13 and 9q13 by fluorescence in situ hybridization". The ...
... sex pre-selection of spermatozoa by albumin separation method evaluated by double-labelled fluorescence in-situ hybridization ...
... are stained with fluorescent in situ hybridization. ... At the fluorescence-microscope level they appear as irregular, ... that is visible using fluorescence microscopy. The actual function of the veil is not clear, although it is excluded from the ...
... on human chromosome 2q14.1 by fluorescence in situ hybridization and chromosome morphometry.". Genomics 29 (3): 817-8. PMID ...
... despite the fact that in situ hybridization studies demonstrated a lower frequency of single Y-bearing sperm than expected and ... In December 1969, Lore Zech at the Karolinska Institute in Stockholm first reported intense fluorescence of the AT-rich distal ...
"Characterization of extensive genetic alterations in ductal carcinoma in situ by fluorescence in situ hybridization and ... Rowley JD (June 1973). "Identification of a translocation with quinacrine fluorescence in a patient with acute leukemia". Ann. ... "Inferring tree models for oncogenesis from comparative genome hybridization data". J. Comput. Biol. 6 (1): 37-51. doi:10.1089/ ... detected both by comparative genomic hybridization (CGH),[32] and array CGH,[38]) and karyotypic variations including ...
... to human chromosome band 15q21.2 by fluorescence in situ hybridization". Cytogenet Cell Genet. 81 (3-4): 292-3. doi:10.1159/ ...
Wikimedia Commons has media related to Fluorescence in situ hybridization.. *Fluorescent+in+Situ+Hybridization at the US ... Fluorescence in situ hybridization (FISH) is a molecular cytogenetic technique that uses fluorescent probes that bind to only ... Fluorescence in situ Hybridization Photos of bacteria. *Rational design of polynucleotide probe mixes to identify particular ... In-Solution Fluorescent In Situ Hybridization and Fluorescence-Activated Cell Sorting for Single Cell and Population Genome ...
... can be used to identify as many labeled features as there are different fluorophores used in the hybridization. ... Multicolor fluorescence in situ hybridization (FISH), in its simplest form, ... Fluorescence in situ Hybridization. Hardware and Software Implications in the Research Laboratory. Nearly a quarter-century has ... Multicolor fluorescence in situ hybridization (FISH), in its simplest form, can be used to identify as many labeled features as ...
Application of fluorescence in situ hybridization in the detection of bladder transitional-cell carcinoma: A multi-center ... Detection of Bladder Cancer in Urine Sediments by a Novel Multicolor Fluorescence In Situ Hybridization (Quartet) Test. Bladder ... We aimed to design a multicolor fluorescence in situ hybridization (FISH) test referred to as the quartet test for the ... The aim of the present study was to estimate the diagnostic value of fluorescence in situ hybridization (FISH) analysis of ...
A Fluorescence in Situ Hybridization System for Karyotyping Soybean. Seth D. Findley, Steven Cannon, Kranthi Varala, Jianchang ... A Fluorescence in Situ Hybridization System for Karyotyping Soybean Message Subject (Your Name) has forwarded a page to you ... A Fluorescence in Situ Hybridization System for Karyotyping Soybean. Seth D. Findley, Steven Cannon, Kranthi Varala, Jianchang ... A Fluorescence in Situ Hybridization System for Karyotyping Soybean. Seth D. Findley, Steven Cannon, Kranthi Varala, Jianchang ...
An in situ hybridization approach to labeling DNA and RNA targets in the specimen using oligonucleotide-based fluorescent ... Fluorescence in situ Hybridization. Referred to as FISH, the technique is used primarily for chromosomal analysis. ... An in situ hybridization approach to labeling DNA and RNA targets in the specimen using oligonucleotide-based fluorescent ...
Genomic in situhybridization (GISH) demonstrates the high level of overall similarity... ... Fluorescence in situ hybridization (FISH) has been used to analyse the structure of the rye B chromosome. ... Fluorescencein situ hybridization (FISH) has been used to analyse the structure of the rye B chromosome. Genomicin situ ... B chromosome DNA sequence composition fluorescencein situ hybridization repetitive sequence Secale cereale translocations ...
... Xingwei Wang,1 Xiaodong ... Fluorescence in situ hybridization (FISH) tests provide promising molecular imaging biomarkers to more accurately and reliably ... In this study, a dual-detector fluorescence image scanning system was applied to scan four specimen slides with FISH-probed ...
Buy Introduction to Fluorescence In Situ Hybridization by Michael Andreeff, Daniel Pinkel from Waterstones today! Click and ... Introduction to Fluorescence In Situ Hybridization: Principles and Clinical Applications (Hardback). Michael Andreeff (editor) ... Introduction to Fluorescence In Situ Hybridization provides a solid groundwork in the basic principles and techniques of FISH ... Introduction to Fluorescence in Situ Hybridization Principles and Clinical Applications Edited by Michael Andreeff, M.D., PH.D ...
F1SH stands for Fluorescence in Situ Hybridization (cancer detection). F1SH is defined as Fluorescence in Situ Hybridization ( ... How is Fluorescence in Situ Hybridization (cancer detection) abbreviated? ... www.acronymfinder.com/Fluorescence-in-Situ-Hybridization-(cancer-detection)-(F1SH).html. *Chicago style: Acronym Finder. S.v. " ... www.acronymfinder.com/Fluorescence-in-Situ-Hybridization-(cancer-detection)-(F1SH).html. *APA style: F1SH. (n.d.) Acronym ...
... on WN Network delivers the latest Videos and Editable pages for News & Events, including ... Fluorescence In Situ Hybridization Industry Overview 1.1 Fluorescence In Situ Hybridization Definition 1.2 Fluorescence In Situ ... Fluorescence in situ hybridization. Fluorescence in situ hybridization (FISH) is a cytogenetic technique that uses fluorescent ... Fluorescence in situ hybridization. Fluorescence in situ hybridization (FISH) is a cytogenetic technique that uses fluorescent ...
Unstimulated bone marrow/blood culture or direct preparation and fluorescence in situ (FISH) hybridization with commercially ...
Here, we report the development of a fluorescence in situ hybridization (FISH) based method for detecting malaria infection in ... Fluorescent in situ hybridization Is the Subject Area "Fluorescent in situ hybridization" applicable to this article? Yes. No. ... Fluorescence in situ hybridization (FISH) is a cytogenetic technique used to detect and localize the specific nucleic acid (DNA ... 2015) Fluorescence In Situ Hybridization (FISH) Assays for Diagnosing Malaria in Endemic Areas. PLoS ONE 10(9): e0136726. https ...
We present a novel method for real-time monitoring and kinetic analysis of fluorescence in situ hybridization (FISH). We ... In Situ Hybridization, Fluorescence. A type of IN SITU HYBRIDIZATION in which target sequences are stained with fluorescent dye ... The simultaneous identification of all chromosomes from a cell by fluorescence in situ hybridization (IN SITU HYBRIDIZATION, ... In this study, investigators assess, using Fluorescence in situ Hybridization (FISH) and Comparative Genomic Hybridization (CGH ...
Identification of spatially associated subpopulations by combining scRNA-seq and sequential fluorescence in situ hybridization ... Identification of spatially associated subpopulations by combining scRNA-seq and sequential fluorescence in situ hybridization ... Identification of spatially associated subpopulations by combining scRNA-seq and sequential fluorescence in situ hybridization ... Identification of spatially associated subpopulations by combining scRNA-seq and sequential fluorescence in situ hybridization ...
... *INTERPHASE FLUORESCENCE IN SITU HYBRIDIZATION ... INTERPHASE FLUORESCENCE IN SITU HYBRIDIZATION TEST FOR HAEMATOLOGICAL MALIGNANCIES. *INTERPHASE FLUORESCENCE IN SITU ... RAPID ANEUPLOIDY SCREENING WITH FLUORESCENCE IN SITU HYBRIDIZATION TEST. *RAPID ANEUPLOIDY SCREENING WITH FLUORESCENCE IN SITU ... INTERPHASE FLUORESENCE IN SITU HYBRIDIZATION PANEL TEST FOR GLIOMA. *INTERPHASE FLUORESENCE IN SITU HYBRIDIZATION PANEL TEST ...
Using spectrally distinct fluorophore labels for each hybridization probe, this approach gives you the power to resolve several ... Multiplex fluorescence in situ hybridization (FISH) enables you to assay multiple targets and visualize colocalized signals in ... Simultaneous detection of expression of five genes in a whole-mount Drosophila embryo by fluorescence in situ hybridization ( ... Four-color fluorescence in situ hybridization on a Drosophila embryo. A late blastoderm stage (nuclear cycle 14) embryo was ...
GFP labelled actin and β-actin fluorescence in situ hybridiation (FISH) images were taken at specific wavelengths with a ... In addition, individual chromosomes are viewed in metaphase using triple-wavelength fluorescence illumination and detection. ... Application Article: Brightfield and Fluorescence Imaging using 3D PrimeSurface Ultra-Low Attachment Microplates ...
Fluorescence in situ hybridization. Revision as of 09:32, 18 March 2009 by Honee_v (Talk , contribs) (updated page) ... Retrieved from "http://www.biology-online.org/bodict/index.php?title=Fluorescence_in_situ_hybridization&oldid=95921" ...
We report an innovative fluorescence in situ hybridization technique which exploits a unique resource of 41 telomere-specific ... Development and clinical application of an innovative fluorescence in situ hybridization technique which detects submicroscopic ...
We evaluated a fluorescence in situ hybridization (FISH) assay, which has previously been shown to be of value for the ... Distinction of conjunctival melanocytic nevi from melanomas by fluorescence in situ hybridization. Authors. *. Klaus J. Busam, ... Gerardo Ferrara, Anna Chiara De Vanna, Fluorescence In Situ Hybridization for Melanoma Diagnosis, The American Journal of ... Distinction of conjunctival melanocytic nevi from melanomas by fluorescence in situ hybridization. ...
Lee SH, Ryu JA, Do GS, Seo BB, Park JH, Kim IS and Song SD (1998) Chromosome analysis by fluorescence in situ hybridization of ... platyphyllum Sequential fluorescence in situ hybridization Informative factor Independent localization This is a preview of ... Lee SH and Seo BB (1997) Chromosomal localization of 5S and 18S-26S rRNA genes using fluorescence in situ hybridization in ... platyphyllum by sequential fluorescence in situ hybridization in accordance with sequence polymorphism. ...
Update on fluorescence in situ hybridization. Arch Pathol Lab Med 2011;135:830-837.. *Web of Science® Times Cited: 18 ... Fluorescence in situ hybridization (FISH) as an ancillary diagnostic tool in the diagnosis of melanoma. Am J Surg Pathol 2009; ... Fluorescence in situ hybridization for ambiguous melanocytic tumors. Histol Histopathol 2012;27:1539-1542.. *CAS, ... Fluorescence in-situ hybridization analysis for melanoma diagnosis. Histopathology 2012;60:706-714.. Direct Link: ...
FISH is a sensitive and useful adjunct to cytogenetic testing for the detection of abnormalities of chromosomal structure or numbers (eg, deletions, translocations, duplications, aneuploidy). It is often the method of choice for detection of microdeletions.. Prenatal: Interphase FISH is a rapid diagnostic test when one of the commonly occurring trisomies (21, 18, 13) or a sex chromosome abnormality is suspected. It is a useful adjunct but cannot replace full karyotyping.. Oncology: FISH can detect a number of translocations (eg, BCR-ABL; MLL; PML/RARA; TEL/AML1) associated with haematological malignancies and can be used to monitor minimal residual disease after chemotherapy and/or bone marrow transplantation.. It can also detect gene amplifications (eg, MYC/MYCN) associated with an adverse prognosis for certain tumours.. Following sex mismatched bone marrow transplantation, FISH can be used to confirm engraftment.. In disorders such as CLL or Plasma cell myeloma where it may be difficult to ...
... ,, Previous Message , Next Message ,, From:. Carol Burden ,[email protected], (by ... [email protected] wrote: Hello Histonetters, I would like to do fluorescence in situ hybridization on paraffin ...
... and comparative genomic hybridization approaches, which have been successfully used to study various aspects of geno ... This manual offers detailed protocols for fluorescence in situ hybridization (FISH) ... This manual offers detailed protocols for fluorescence in situ hybridization (FISH) and comparative genomic hybridization ... Fluorescence In Situ Hybridization onto DNA Fibres Generated Using Molecular Combing Sandra Louzada, Jun Komatsu, Fengtang Yang ...
Comparative Study between Quantitative Digital Image Analysis and Fluorescence In Situ Hybridization of Breast Cancer Equivocal ... usually by fluorescence in situ hybridization (FISH) investigations. It still remains on open question, whether quantitative ... of quantitative digital analysis of IHC stained slides and compare its performance to fluorescence in situ hybridization in ...
The application of fluorescence in situ hybridization (FISH) technology in diagnosis and molecular classification of cancer- ... Tian E. (2018) Fluorescence In Situ Hybridization (FISH) in Multiple Myeloma. In: Heuck C., Weinhold N. (eds) Multiple Myeloma ... The application of fluorescence in situ hybridization (FISH) technology in diagnosis and molecular classification of cancer- ... Flactif M, Zandecki M, Lai JL, Bernardi F, Obein V, Bauters F, Facon T (1995) Interphase fluorescence in situ hybridization ( ...
In Situ Hybridization, Fluorescence / methods*. Indoles. Karyotyping / methods. Mitosis. Mitosporic Fungi / ultrastructure*. ... Fluorescence in situ hybridization (FISH) was also successfully applied to the specimens, revealing one ribosomal RNA gene ...
Larracuente, A. M., Ferree, P. M. Simple Method for Fluorescence DNA In Situ Hybridization to Squashed Chromosomes. J. Vis. Exp ... Pardue, M. L. In situ hybridization to polytene chromosomes in Drosophila using digoxigenin-labeled probes. Cold Spring Harbor ... Blattes, R., Kas, E. Fluorescent in situ hybridization (FISH) on diploid nuclei and mitotic chromosomes from Drosophila ... Pimpinelli, S., Bonaccorsi, S., Fanti, L., Gatti, M. Fluorescent in situ hybridization (FISH) of mitotic chromosomes from ...
  • Fluorescence in situ hybridization ( FISH ) is a molecular cytogenetic technique that uses fluorescent probes that bind to only those parts of a nucleic acid sequence with a high degree of sequence complementarity . (wikipedia.org)
  • Fluorescence microscopy can be used to find out where the fluorescent probe is bound to the chromosomes. (wikipedia.org)
  • The power of in situ hybridization can be greatly extended by the simultaneous use of multiple fluorescent colors. (microscopyu.com)
  • To overcome this obstacle, we screened soybean repetitive DNA to develop a cocktail of fluorescent in situ hybridization (FISH) probes that could differentially label mitotic chromosomes in root tip preparations. (genetics.org)
  • An in situ hybridization approach to labeling DNA and RNA targets in the specimen using oligonucleotide-based fluorescent probes. (microscopyu.com)
  • and fluorescent in situ hybridization to detect chromosomal sequences. (wn.com)
  • Fluorescent in situ hybridization can be used as a complementary assay for the diagnosis of Tropheryma whipplei infection. (bioportfolio.com)
  • Primary objective of the study is to evaluate the correlation between level of HER2-neu gene amplification evalued by dual-color Fluorescent in-situ hybridization (FISH) test and time to p. (bioportfolio.com)
  • Do GS, Seo BB, Ko JM, Lee SH, Park JH, Kim IS and Song SD (1999) Analysis of Somaclonal variation through tissue culture and chromosomal localization of rDNA sites by fluorescent in situ hybridization in wild Allium tuberosum and a regenerated variant. (springer.com)
  • The peptide nucleic acid fluorescence in situ hybridization (PNA FISH) assay is a rapid test that utilizes fluorescent-labeled peptide nucleic acid probes targeting the specific rRNA sequences of Candida albicans ( 5 , 9 , 12 ). (asm.org)
  • A type of IN SITU HYBRIDIZATION in which target sequences are stained with fluorescent dye so their location and size can be determined using fluorescence microscopy. (umassmed.edu)
  • Cornejo KM, Hutchinson L, Cyr MS, Nose V, McLaughlin PJ, Iafrate AJ, Sadow PM. MYC Analysis by Fluorescent In Situ Hybridization and Immunohistochemistry in Primary Adrenal Angiosarcoma (PAA): a Series of Four Cases. (umassmed.edu)
  • In this way, if fluorochrome-labeled tyramides are used, numerous fluorescent molecules can be introduced at the hybridization site in situ. (asm.org)
  • Microelectrodes and fluorescent in situ hybridization (FISH) in biofilm research have been used to investigate the spatial distributions of various microbial activities in biofilms and have led to new experimental findings as well as modifications of the homogeneous assumptions in the biofilm kinetic models. (iwaponline.com)
  • Several decades later, primary antibody-dependent fluorescence detection of nucleic acid hybrids was achieved, however, this technique was soon replaced by the appearance of fluorescent nucleic acid probes.In situ hybridization, first performed in the late 1960s, was not fluorescent at all, but used a probe labeled with a radioactive isotope. (abnewswire.com)
  • The first application of fluorescence in-situ detection was in the early 1980s, detection of nick translations characterized by secondary detection of biotinylated probes and fluorescent streptavidin conjugates was used to detect DNA and mRNA targets. (abnewswire.com)
  • About a decade later, improved labeling of synthetic single-stranded DNA probes allows the chemical preparation of hybridization probes carrying enough fluorescent molecules to allow direct detection. (abnewswire.com)
  • Fluorescent specimens can be viewed using a compound microscope fitted (usually) with an epifluorescent lamp (for epi-illumnation, the light is both projected and detected through the objective lens), filter blocks which contain dichroic beam-splitting mirrors, and objectives designed to transmit fluorescence (see Subheading 3.2.1. (alpfmedical.info)
  • The hybridization period continues for about 24-hours, after which the specimen is ready to be viewed under a fluorescent microscope. (fertilitysmarts.com)
  • FISH involves the hybridization of a target DNA sequence labeled with a fluorescent dye (called a probe). (uconn.edu)
  • Fluorescence in situ hybridization (FISH) is a kind of ISH which uses fluorescent probes binding parts of the chromosome to show a high degree of sequence complementarity. (creative-diagnostics.com)
  • On the other hand, non-radioactive successfully used with in situ hybridization include digoxigenin (DIG), biotin and fluorescent labels. (creative-diagnostics.com)
  • The key techniques currently in use include in situ hybridization to mRNA with oligonucleotide and RNA probes (both radio-labeled and hapten-labeled), analysis with light and electron microscopes, whole mount in situ hybridization, double detection of RNAs and RNA plus protein, and fluorescent in situ hybridization to detect chromosomal sequences. (wikipedia.org)
  • Then, the probe that was labeled with either radio-, fluorescent- or antigen-labeled bases (e.g., digoxigenin) is localized and quantified in the tissue using either autoradiography, fluorescence microscopy, or immunohistochemistry, respectively. (wikipedia.org)
  • Multicolor fluorescence in situ hybridization ( FISH ), in its simplest form, can be used to identify as many labeled features as there are different fluorophores used in the hybridization. (microscopyu.com)
  • To evaluate the diagnostic value of fluorescence in situ hybridization (FISH) in bladder cancer. (urotoday.com)
  • We aimed to design a multicolor fluorescence in situ hybridization (FISH) test referred to as the quartet test for the detection of bladder cancer in urine. (urotoday.com)
  • The aim of the present study was to estimate the diagnostic value of fluorescence in situ hybridization (FISH) analysis of tumor cells in voided urine specimens for detecting upper tract urothelial carcinoma (UTUC). (urotoday.com)
  • We compared PTEN immunohistochemistry (IHC) and PTEN fluorescence in situ hybridization (FISH) in the largest existing radical prostatectomy cohort with clinical follow-up data. (urotoday.com)
  • Fluorescence in situ hybridization (FISH) has been used to analyse the structure of the rye B chromosome. (springer.com)
  • Fluorescence in situ hybridization (FISH) tests provide promising molecular imaging biomarkers to more accurately and reliably detect and diagnose cancers and genetic disorders. (hindawi.com)
  • In this study, a dual-detector fluorescence image scanning system was applied to scan four specimen slides with FISH-probed chromosome X. A CAD scheme was developed to detect analyzable interphase cells and map the multiple imaging slices recorded FISH-probed signals into the 2-D projection images. (hindawi.com)
  • Introduction to Fluorescence in Situ Hybridization Principles and Clinical Applications Edited by Michael Andreeff, M.D., PH.D., and Daniel Pinkel, PH.D. Fluorescence In Situ Hybridization (FISH) has become an essential tool in the diagnosis and management of a variety of solid tumors and hematologic malignancies in the clinical setting, as well as an aid in the identification of particular genetic disorders. (waterstones.com)
  • Introduction to Fluorescence In Situ Hybridization provides a solid groundwork in the basic principles and techniques of FISH and covers in detail the applications of this technology to cancer in humans, including tumorigenesis, prostate and breast tumors, myeloid leukemias, and lymphoproliferative malignancies. (waterstones.com)
  • Here, we report the development of a fluorescence in situ hybridization (FISH) based method for detecting malaria infection in blood smears and describe the use of an LED light source that makes the method suitable for use in resource-limited malaria endemic countries. (plos.org)
  • Strand-specific fluorescence in situ hybridization: the CO-FISH family. (nih.gov)
  • Standard FISH methodologies require functionally single-stranded DNAs in order to facilitate hybridization between the probe and the complementary chromosomal target sequence. (nih.gov)
  • Real-Time Monitoring of Fluorescence in situ Hybridization (FISH) Kinetics. (bioportfolio.com)
  • To compare the relative effectiveness of fluorescence in situ hybridization (FISH) and cytology in diagnosing upper urinary tract urothelial carcinoma (UUT-UC) and to evaluate the advantages and poten. (bioportfolio.com)
  • Graphene oxide-quenching-based fluorescence in situ hybridization (G-FISH) to detect RNA in tissue: Simple and fast tissue RNA diagnostics. (bioportfolio.com)
  • In this study, investigators assess, using Fluorescence in situ Hybridization (FISH) and Comparative Genomic Hybridization (CGH) arrays for Preimplantation Genetic Screening (PGS), the inc. (bioportfolio.com)
  • Fluorescence In Situ Hybridization using direct-labelled FISH DNA probes are hybridized to target loci and analyzed under fluorescence microscopy. (sgh.com.sg)
  • Multiplex fluorescence in situ hybridization (FISH) enables you to assay multiple targets and visualize colocalized signals in a single specimen. (thermofisher.com)
  • RNA fluorescence in situ hybridization (FISH) for Cre mRNA in genetically identical cells in which expression of Cre is epigenetically regulated. (berkeley.edu)
  • We evaluated a fluorescence in situ hybridization (FISH) assay, which has previously been shown to be of value for the diagnosis of melanocytic nevi and melanomas of the skin, using probes targeting 6p25 (RREB1), 6q23 (MYB), 11q13 (CCND1) and centromere 6 (CEP6), for its potential to assist in the distinction of conjunctival melanocytic nevi from melanomas. (wiley.com)
  • According to the College of American Pathologists/American Society of Clinical Oncology guidelines equivocal HER2/neu score 2+ cases are subject for further testing, usually by fluorescence in situ hybridization (FISH) investigations. (hindawi.com)
  • The application of fluorescence in situ hybridization (FISH) technology in diagnosis and molecular classification of cancer-risk has become an essential tool in the proceeding of personalized therapy. (springer.com)
  • Flactif M, Zandecki M, Lai JL, Bernardi F, Obein V, Bauters F, Facon T (1995) Interphase fluorescence in situ hybridization (FISH) as a powerful tool for the detection of aneuploidy in multiple myeloma. (springer.com)
  • Fluorescence in situ hybridization (FISH) was also successfully applied to the specimens, revealing one ribosomal RNA gene cluster, or nucleolus organizer region (NOR) in the genome of each fungus. (biomedsearch.com)
  • Growing prevalence of target diseases leads to clinical urgency for adoption of rapid diagnostic alternatives such as Fluorescence In Situ Hybridization (FISH) imaging systems, which is anticipated to fuel demand in the coming years. (grandviewresearch.com)
  • When looking at how many copies are incorporated and where within the genome we use a technique called FISH which is fluorescence in-situ hybridisation, and I will take a biopsy from the animal and grow up cells back into culture and then arrest them at metaphase, prepare some slides with those cells, and then I will probe my slides for the transgene. (sciencelearn.org.nz)
  • Towards a cellular multi-parameter analysis platform: fluorescence in situ hybridization (FISH) on microhole-array chips. (sigmaaldrich.com)
  • Fluorescence in situ hybridization (FISH) is a technique used in the diagnostics to determine the localization and the presence or absence of specific DNA sequence. (sigmaaldrich.com)
  • Furthermore, FISH on FNAs gave us better hybridization signals than their corresponding FFPE tissue sections. (scirp.org)
  • Here, we used histology and fluorescence in situ hybridization (FISH) to detect and localize bacteria in the tissues of P. clavata. (archives-ouvertes.fr)
  • DNA fluorescence in situ hybridization (FISH) is a powerful cytogenetic assay, but conventional sample-preparation methods for FISH do not support large-scale high-throughput data acquisition and analysis, which are potentially useful for several biomedical applications. (rsc.org)
  • Although fluorescence in situ hybridization (FISH) and microsatellite-based polymerase chain reaction (PCR) for loss of heterozygosity (LOH) are common methods to test for 1p/19q codeletion, it is unclear which test is better at prognostic stratification. (nih.gov)
  • Immunohistochemistry (IHC), fluorescence in situ hybridization (FISH), and various other molecular markers (including epigenetic markers) have sufficient evidence behind their use however, some are still in their research stages, or yet to be used widely in clinical diagnosis. (frontiersin.org)
  • The impact of rapid identification of Candida albicans blood isolates by peptide nucleic acid fluorescence in situ hybridization (PNA FISH) on the selection and expenditure of antifungal therapy was evaluated. (asm.org)
  • To assess the feasibility of characterizing gene copy number alteration by fluorescence in situ hybridization (FISH) of circulating tumor cells (CTC) isolated using the CellSearch system in patients with progressive castration-resistant metastatic prostate cancer. (aacrjournals.org)
  • In situ uptake of [2,4,6,7-3H(N)]estrone ([3H]E1) by the major phylogenetic groups present in activated sludge samples from two different municipal wastewater treatment plants was investigated using microautoradiography-fluorescence in situ hybridization (MAR-FISH). (biomedsearch.com)
  • Following FISH with 45S rDNA as a probe, we detected seven 45S rDNA hybridization sites in the diploid L. perenne cv. (nih.gov)
  • Usually, karyotyping and fluorescence in situ hybridization (FISH) analysis are used to detect these abnormalities in resected tumor tissues. (aacrjournals.org)
  • We tested a previously described protocol for fluorescence in situ hybridization of marine bacterioplankton with horseradish peroxidase-labeled rRNA-targeted oligonucleotide probes and catalyzed reporter deposition (CARD-FISH) in plankton samples from different lakes. (asm.org)
  • Pune, Maharashtra, India, February 22 2021 (Wiredrelease) Brandessence Market Research and Consulting Pvt ltd -:A recent report on Fluorescence In Situ Hybridization (FISH) Imaging Systems Market provides a detailed analysis of the industry size, revenue forecasts and geographical landscape pertaining to this business space. (pharmiweb.com)
  • Fluorescence In Situ Hybridization (FISH) Imaging Systems allows very precise spatial resolution of morphological and genomic structures. (pharmiweb.com)
  • The application of Fluorescence In Situ Hybridization (FISH) is growing rapidly in tumor biology, radiation labels, genomics, cytogenetics, prenatal research, gene mapping, gene amplification and basic biomedical research. (pharmiweb.com)
  • So, during the study of Global Fluorescence In Situ Hybridization (FISH) Imaging Systems market, we have considered Fluorescence In Situ Hybridization (FISH) Imaging Systems products and consumables to analyze the market. (pharmiweb.com)
  • Global Fluorescence In Situ Hybridization (FISH) Imaging Systems Market report is segmented on the basis of Product type, Application Type, End User type and by regional & country level. (pharmiweb.com)
  • Based on Product type global Fluorescence In Situ Hybridization (FISH) Imaging Systems Market is classified as Instruments, Consumables & Accessories and Software. (pharmiweb.com)
  • Based upon Application type, global Fluorescence In Situ Hybridization (FISH) Imaging Systems Market is classified as Cancer, Genetic disorders, Biological Research and others. (pharmiweb.com)
  • Based upon End User type, global Fluorescence In Situ Hybridization (FISH) Imaging Systems Market is classified as Diagnostic Laboratories, Research Centers and others. (pharmiweb.com)
  • The regions covered in this Fluorescence In Situ Hybridization (FISH) Imaging Systems Market report are North America, Europe, Asia-Pacific and Rest of the World. (pharmiweb.com)
  • Improvements in diagnostic testing, increasing number of cancer patients, technological improvements in imaging techniques and automation are major key drivers for the growth of the Global Fluorescence In Situ Hybridization (FISH) Imaging Systems Market. (pharmiweb.com)
  • Modern advancements in Fluorescence In Situ Hybridization (FISH) technology involve various methods for refining probe labeling efficiency and the use of super resolution imaging systems for direct visualization of intra-nuclear chromosomal organization and profiling of RNA transcription in single cells. (pharmiweb.com)
  • A new advancement in hybridization technique called as CASFISH (Cas9-mediated FISH) allows in situ labeling of single-copy sequences and repetitive sequences without disrupting nuclear genomic organization. (pharmiweb.com)
  • Therefore technological advancements is majorly responsible for the growth of Global Fluorescence In Situ Hybridization (FISH) Imaging Systems Market. (pharmiweb.com)
  • Fluorescence in situ hybridization (FISH) of EGFR and HER2 has been proposed as an alternative method of analysis. (aacrjournals.org)
  • This project aimed to determine the optimal scoring method for FISH or chromogenic in situ hybridization (CISH) assays when analyzing small NSCLC samples to predict response. (aacrjournals.org)
  • Recent reports have described the use of fluorescence in situ hybridization (FISH) to assess the status of the EGFR gene in gefitinib responders with NSCLC versus nonresponders ( 13 - 15 ). (aacrjournals.org)
  • Background: Testing for human epidermal growth factor receptor-2 (HER-2) is routinely performed after breast cancer diagnosis by either immunohistochemistry (IHC) or fluorescence in situ hybridization (FISH). (aacrjournals.org)
  • Fluorescence in situ hybridization (FISH) with horseradish peroxidase (HRP)-labeled oligonucleotide probes and tyramide signal amplification, also known as catalyzed reporter deposition (CARD), is currently not generally applicable to heterotrophic bacteria in marine samples. (asm.org)
  • The enhanced fluorescence intensities and signal-to-background ratios make CARD-FISH superior to FISH with directly labeled oligonucleotides for the staining of bacteria with low rRNA content in the marine environment. (asm.org)
  • Fluorescence in situ hybridization (FISH) of bacteria was first described more than a decade ago ( 2 , 11 ) and was hailed as a breakthrough for microbial ecology. (asm.org)
  • In our previous study, we demonstrated that high percentage (47%) of UMs express the type-I receptor for Luteinizing Hormone-Releasing Hormone (LHRH-R). The gene encoding LHRH-R is harbored by chromosome 4q21.2, however the numerical aberrations of this chromosome 4 have never been studied by fluorescence in situ hybridization (FISH) in UM. (arvojournals.org)
  • In this study, we used immunohistochemistry (IHC) and fluorescence in situ hybridisation (FISH) to assess HER2 status in specimens from patients with gastric cancer. (termedia.pl)
  • Archived nail apparatus melanomas were analyzed by fluorescence in situ hybridization (FISH) using probes targeting the genes at 6p25 (RREB1), 11q13 (CCND1), 8q24.1 (MYC), 6q23 (MYB), 9p21 (CDKN2A) and the centromeres of chromosomes 8 (D8Z2) and 6 (D6Z1). (ovid.com)
  • We also showed that a four-target fluorescence in situ hybridization (FISH) assay was suitable for detecting abnormal cells in sputum samples from a small set of cases and controls nested within our ongoing cohort ( 20 ). (aacrjournals.org)
  • Recent revision of the lissencephaly critical region on chromosome 17p13.3 and confirmation of LIS1 as the causative gene for classical lissencephaly has allowed the development and application of fluorescence in situ hybridization (FISH) probes corresponding directly to this gene. (ovid.com)
  • We have coupled fluorescence in situ hybridization (FISH) with Raman microscopy for simultaneous cultivation-independent identification and determination of 13C incorporation into microbial cells. (nerc.ac.uk)
  • Cellular 13C-content determinations exhibited good congruence between fresh cells and FISH hybridized cells indicating that spectral peaks, including phenylalanine resonance, which were used to determine 13C-labelling, were preserved during fixation and hybridization. (nerc.ac.uk)
  • Aims: Fluorescence in situ hybridization (FISH) can be used to reveal several genomic imbalances relevant to proper cancer diagnosis and to the correct treatment regime. (lu.se)
  • However, FISH requires expensive and advanced fluorescence microscopes in addition to expertise in fluorescence microscopy. (lu.se)
  • To determine whether a newly developed dual-colour chromogenic in situ hybridization (CISH) method is a suitable alternative to FISH, we analysed the human epidermal growth factor receptor 2 gene (HER2) amplification level of 168 breast cancer specimens using dual-colour CISH and FISH and compared the results. (lu.se)
  • Therefore, a series of antibodies was evaluated to assess their immunohistochemical (IHC) sensitivity in correlation to gene amplification determined by fluorescence in situ hybridization (FISH). (arctichealth.org)
  • FISH need additional equipment for analysis such as fluorescence microscopy and multiband fluorescence filters. (powershow.com)
  • The fluorescence in situ hybridization (FISH) is the gold standard in human epidermal growth factor receptor 2 (HER2) status assessment in breast cancer. (epfl.ch)
  • The objective of this study is to try the combination of two methods, both FISH and microelectrode measurements, and to provide reliable and in situ information on nitrifying bacterial activity in biofilms. (iwaponline.com)
  • UroVysion ® is a multitarget fluorescence in situ hybridization (FISH) test for bladder cancer detection. (pubmedcentralcanada.ca)
  • Clinical implementation of fluorescence in situ hybridization (FISH) in cytogenetic laboratories allows for high-resolution analysis of recurrent structural chromosomal rearrangements specific to AML, especially in AML with normal karyotypes, which comprises approximately 33-50% of AML-positive specimens. (springermedizin.de)
  • The 5S and 45S rDNA genes were physically mapped on the metaphase chromosomes using fluorescence in situ hybridization (FISH). (fao.org)
  • The steps of denaturation, hybridisation and detection are the same as those used in a uni-colour FISH experiment. (gla.ac.uk)
  • We describe our experience of using a panel of fluorescence in situ hybridisation (FISH) probes on formalin-fixed paraffin-embedded tissue sections in the diagnostic work-up of 162 patients with non-Burkitt high grade B-cell non-Hodgkin's lymphomas (HG-BNHL). (bmj.com)
  • This study evaluates the concordance between HER2 gene amplification in invasive breast cancer determined by fluorescence in situ hybridisation (FISH) and a new silver enhanced in situ hybridisation (SISH) technique. (uzh.ch)
  • Using fluorescence in situ hybridization (FISH) we localized introduced genes on metaphase chromosomes of barley, wheat, and triticale transformed by microprojectile bombardment of microspores and scutellar tissue with the pDB1 plasmid containing the uidA and bar genes. (dtu.dk)
  • The overall HER2 fluorescence in situ hybridization (FISH) amplification, non-amplification and equivocation rates in HER2 IHC-2+ cases were 17.8%, 76.2% and 6.0%, respectively. (jcancer.org)
  • A total of fourteen repetitive sequences were used as probes for fluorescence in situ hybridization (FISH) with the aim of identifying inter- and intra-species polymorphisms. (readbyqxmd.com)
  • Detect and enumerate FISH break-apart and fusion events involving up to 7 fluorescence target signals. (leicabiosystems.com)
  • Correlation of cytogenetic and fluorescence in situ hybridization (FISH) studies in normal and gliotic brain. (semanticscholar.org)
  • We carried out fluorescence in situ hybridisation (FISH) on three different myeloid leukaemia-derived cell lines (GDM-1, GF-D8 and K562). (brunel.ac.uk)
  • The probes used in these experiments were whole and partial chromosome paints, Multiplex-fluorescence in situ hybridisation (M-FISH) probes as well as locus specific probes for the 7q22, 7q31 and 7q36 regions. (brunel.ac.uk)
  • A cDNA clone of this gene was used as a probe to identify three genomic bacterial artificial chromosome (BAC) clones, one of which was used as a probe to map hTERT by fluorescence in situ hybridization (FISH) to chromosome 5p15.33. (gla.ac.uk)
  • Increased hTERC gene expression has been frequently observed and amplification was shown using fluorescence in situ hybridization (FISH) in different cancers. (tjh.com.tr)
  • We present a case of mucoepidermoid carcinoma with t(3;12)(q24;p13) and polysomy X by cytogenetic and fluorescence in situ hybridization (FISH) techniques. (nih.gov)
  • Pml/rara fusion transcript and a complex translocation involving chromosomes 5, 15 and 17 were detected by fluorescence in situ hybridization (FISH) technique which was applied as in adjunct to conventional cytogenetics. (tjh.com.tr)
  • We report the third case of this aggressive lymphoma in a child as well as additional support for the consistency of the recently discovered cytogenetic abnormalities, isochromosome 7q and trisomy 8, which in this case were documented using fluorescence in situ hybridization (FISH) of a touch- preparation of the spleen. (elsevier.com)
  • Definition - What does Fluorescence In Situ Hybridization (FISH) mean? (fertilitysmarts.com)
  • Fluorescence in situ hybridization (FISH) is a technique used to spot chromosomal abnormalities that are barely discernible by standard chromosomal testing. (fertilitysmarts.com)
  • Fluorescence in situ hybridization (FISH) allows for the identification of cryptic or complex chromosomal abnormalities. (uconn.edu)
  • Fluorescence in situ hybridization (FISH) detects the presence/absence or location of a specific gene or chromosome at ~ 100kb resolution. (uconn.edu)
  • Color fluorescence photomicrographs of representative FISH findings are provided. (uconn.edu)
  • Detection of chromosomal numerical abnormalities in clinical breast tumour fine-needle aspirations by fluorescence in situ hybridisation (FISH): refinement of a method. (qub.ac.uk)
  • The basic principles for FISH and all other methods of in situ hybridization are the same, except one is utilizing a fluorescence probe to detect specific nucleotide sequences within cells and tissues. (creative-diagnostics.com)
  • DNA or RNA sequences from appropriate, chromosome-specific probes are first labeled with reporter molecules, which are later identified through fluorescence microscopy. (microscopyu.com)
  • When single-stranded probes are then hybridized to such targets, the resulting strand-specific hybridization is capable of revealing a level of information previously unattainable at the cytogenetic level. (nih.gov)
  • Fluorescence in situ hybridization assays using a multiprobe set consisting of centromeric probes for chromosomes 3, 7 and 17 and p16 gene can be used as a diagnostic tool in detecting the genetic alterations in cholangiocarcinoma. (sgh.com.sg)
  • [email protected] wrote: Hello Histonetters, I would like to do fluorescence in situ hybridization on paraffin embedded sections using RNA probes. (histosearch.com)
  • Here, we provide the protocols for the creation of custom-made DNA probes and for conducting hybridizations on various targeting cells and tissues. (springer.com)
  • Multiple colors by fluorescence in situ hybridization using ratio‐labelled DNA probes create a molecular karyotype. (currentprotocols.com)
  • This approach is based on hybridization with horseradish peroxidase (HRP)-labeled oligonucleotide probes and subsequent tyramide signal amplification ( 4 ). (asm.org)
  • In the present study a series of permanent cell lines and patient samples was investigated using a flow cytometric approach for telomere length determination based on in situ hybridization using peptide nucleic acid probes and DNA staining. (diva-portal.org)
  • Subsequent signal amplification is predicated on specific hybridization of adjacent probes (individual oligonucleotides [oligos] that bind side by side on RNA targets). (wikipedia.org)
  • Chen M, Li Y, Ming Z, Biao A. Comparison of HER2 status by fluorescence in situ hybridisation and immunohistochemistry in gastric cancer. (termedia.pl)
  • Her-2/neu analysis in archival tissue samples of human breast cancer: comparison of immunohistochemistry and fluorescence in situ hybridization. (arctichealth.org)
  • Chromogenic in situ hybridization uses a simple immunohistochemistry-like peroxidase reaction. (powershow.com)
  • Ninety-two cases of pediatric BL were retrospectively analyzed for clinical features, immunohistochemistry, EBV-encoded RNA (EBER) status by in situ hybridization and c-myc gene rearrangement by fluorescence in situ hybridization. (nih.gov)
  • Application of fluorescence in situ hybridization in the detection of bladder transitional-cell carcinoma: A multi-center clinical study based on Chinese population. (urotoday.com)
  • Detection of Bladder Cancer in Urine Sediments by a Novel Multicolor Fluorescence In Situ Hybridization (Quartet) Test. (urotoday.com)
  • A comprehensive comparison of fluorescence in situ hybridization and cytology for the detection of upper urinary tract urothelial carcinoma: A systematic review and meta-analysis. (bioportfolio.com)
  • Direct detection of Staphylococcus aureus in positive blood cultures through molecular beacon-based fluorescence in situ hybridization. (bioportfolio.com)
  • T. Iman, S. Amani and A. Mona, "Detection of HER-2/neu Amplification on Fine Needle Aspirates of Breast Cancer Using Fluorescence in Situ Hybridization," Journal of Cancer Therapy , Vol. 4 No. 7A, 2013, pp. 41-48. (scirp.org)
  • Detection of chimeric BCR‐ABL genes on bone marrow samples and blood smears in chronic myeloid and acute lymphoblastic leukemia by in situ hybridization. (currentprotocols.com)
  • Detection of chromosome aberrations in the human interphase nucleus by visualization of specific target DNAs with radioactive and non‐radioactive in situ hybridization techniques: Diagnosis of trisomy 18 with probe L1.84. (currentprotocols.com)
  • Detection of complete and partial chromosome gains and losses by comparative genomic in situ hybridization. (currentprotocols.com)
  • Most bacteria in aquatic habitats are small, slow growing, or starving ( 31 ), and the signal intensities of hybridized bacterioplankton cells were frequently below the detection limits or lost in high background fluorescence. (asm.org)
  • The results obtained from hybridisation of an unselected series of 20 uncultured lymphocytes and 27 uncultured amniocytes indicate that the technique is reliable and can be used for simultaneous detection of major chromosome aneuploidies. (gla.ac.uk)
  • Cell permeabiliza-tion for catalyzed reporter deposition fluorescence in situ detection and the hybridization conditionswere optimized on enrichment cultures of the target species N. devanaterra, as well as the non-targetammonia-oxidizing archaeon Nitrosopumilus maritimus. (csic.es)
  • Aperio Image Analysis offers solutions for automated detection and counting of target signals, with options for brightfield and fluorescence. (leicabiosystems.com)
  • books to read Improved Filter Method for Urine Sediment Detection of Urothelial Carcinoma by Fluorescence in Situ Hybridization (Report). (bookaride.net)
  • In addition to the advantages of detection in morphologic context, in situ hybridization has become popular also because of its high sensitivity in nucleic acid detection. (creative-diagnostics.com)
  • An alternative technology, branched DNA assay, can be used for RNA (mRNA, lncRNA, and miRNA ) in situ hybridization assays with single molecule sensitivity without the use of radioactivity. (wikipedia.org)
  • Over the past 15 years, however, a revolution in light microscopy has occurred through the development of fluorescence techniques that allow unprecedented ease, precision, and accuracy in locating, identifying, and recording data on the genetic makeup of biomedical samples. (microscopyu.com)
  • After washing and signal amplification, the specimen is screened for the reporter molecules by fluorescence microscopy. (microscopyu.com)
  • At the end of the assay the tissue samples are visualized under a fluorescence microscope. (wikipedia.org)
  • Fluorescence in situ hybridization, which is a selective assay for the localization of a specific nucleic acid sequence in its natural context. (abnewswire.com)
  • However, due to this assay cannot benefit from the preservation of tissue structures or cell structures, its future applications are more likely to be in silico than in situ. (abnewswire.com)
  • Multi-target interphase fluorescence in situ hybridization assay increases sensitivity of sputum cytology as a predictor of lung cancer. (nih.gov)
  • Nearly a quarter-century has passed since the first research articles introducing in situ hybridization as a method of detecting and studying DNA sequences in chromosomes and cells appeared in the literature. (microscopyu.com)
  • Genomic in situ hybridization (GISH) demonstrates the high level of overall similarity between A and B chromosomes of rye, as well as the presence of a number of specific sequences. (springer.com)
  • Cuadrado A, Jouve N (1994) Highly repetitive sequences in B-chromosomes of Secale cereale revealed by fluorescence in situ hybridization. (springer.com)
  • Maluszynska J, Schweitzer D (1989) Ribosomal RNA genes in B-chromosomes of Crepis capillaris detected by non-radioactive in situ hybridization. (springer.com)
  • In situ hybridization is used to reveal the location of specific nucleic acid sequences on chromosomes or in tissues, a crucial step for understanding the organization, regulation, and function of genes. (wn.com)
  • Visualization of mitotic chromosomes in filamentous fungi by fluorescence staining and fluorescence in situ hybridization. (biomedsearch.com)
  • To assess the clinical utility of fluorescence in-situ hybridisation with chromosomes 13, 18, 21, X and Y as a stand-alone test in detecting chromosomal abnormalities, and the types of chromosomal abnormalities missed. (hkmj.org)
  • When comparing fluorescence in-situ hybridisation data with karyotype results for the five chromosomes of interest, the sensitivity and specificity were 99.3% and 99.9%, respectively. (hkmj.org)
  • Fluorescence in situ hybridization studies showed the presence of a normal copy number for chromosomes 7, 17, 3q, and 3p. (springermedizin.de)
  • The aim of this work is to assess utility of quantitative digital analysis of IHC stained slides and compare its performance to fluorescence in situ hybridization in cases of breast cancer with equivocal HER2 score 2+. (hindawi.com)
  • Genomic aberrations in mantle cell lymphoma detected by interphase fluorescence in situ hybridization. (haematologica.org)
  • The hybridization reaction identifies, or labels, target genomic sequences so their location and size can be studied. (microscopyu.com)
  • Comparative genomic hybridization for molecular cytogenetic analysis of solid tumors. (currentprotocols.com)
  • We applied fluorescence in situ hybridization to CTC to analyze the androgen receptor ( AR ) and MYC genomic copy number in samples from 77 patients with progressive castration-resistant prostate cancer. (aacrjournals.org)
  • Design and Methods To determine the incidence and clinical relevance of these aberrations, we analyzed 103 well-characterized samples of mantle cell lymphoma by fluorescence in situ hybridization for the most common recurrent additional genomic findings. (haematologica.org)
  • Zurück zum Zitat Adam J, Couturier J, Molinie V, Vieillefond A, Sibony M (2011) Clear-cell papillary renal cell carcinoma: 24 cases of a distinct low-grade renal tumour and a comparative genomic hybridization array study of seven cases. (springermedizin.de)
  • Cytogenetic data of prenatal specimens and results of fluorescence in-situ hybridisation of 5883 patients performed between January 2000 and August 2007 were reviewed. (hkmj.org)
  • Common methods of preparing tissue sections for in-situ hybridization processing include cutting specimens with a cryostat or a Compresstome tissue slicer. (wikipedia.org)
  • In situ Hybridization (ISH) is a method that allows to localize and detect nucleic acid sequences within structurally intact cells or morphologically preserved tissues sections. (creative-diagnostics.com)
  • From a chemical point of view, common precipitating fixatives (such as acetic acid/ethanol and Carnoy's fixative) are not recommended because of a fear that such fixatives would make the cell matrix impermeable, or alter the target nucleic acid to a point that hybridization would be reduced or prevented. (creative-diagnostics.com)
  • To investigate cytogenetic evolution after upfront autologous stem cell transplantation for newly diagnosed myeloma we retrospectively analyzed fluorescence in situ hybridization results of 128 patients with paired bone marrow samples from the time of primary diagnosis and at relapse. (haematologica.org)
  • Rapid aneuploidy screening with fluorescence in-situ hybridisation: is it a sufficiently robust stand-alone test for prenatal diagnosis? (hkmj.org)
  • Institutions supporting fluorescence in-situ hybridisation as a stand-alone test must seriously consider the risks of a missed diagnosis. (hkmj.org)
  • Signal amplification is achieved via series of sequential hybridization steps. (wikipedia.org)
  • The development of in situ techniques provides us with valuable information about the location and expression patterns of genes in individual cells. (abnewswire.com)
  • Identification of spatially associated subpopulations by combining scRNAseq and sequential fluorescence in situ hybridization data. (nih.gov)
  • Fluorescence in situ hybridization with 45S rDNA as the probe shows that 45S rDNAs (green) are the sites of chromosome lesions in meristematic cells of root tips in diploid Lolium perenne cv. (nih.gov)
  • [9] The hybridization signals for each probe when a nucleic abnormality is detected. (wikipedia.org)
  • The probe must be large enough to hybridize specifically with its target but not so large as to impede the hybridization process. (wikipedia.org)
  • Advances in probe and microscope technology have led to the rapid development of techniques for fluorescence over the past decade. (microscopyu.com)
  • However, in situ hybridization requires that many steps be taken with precise optimization for each tissue examined and for each probe used. (wn.com)
  • Using spectrally distinct fluorophore labels for each hybridization probe, this approach gives you the power to resolve several genetic elements or multiple gene expression patterns through multicolor visual display. (thermofisher.com)
  • Probe specificity was improved with a competi-tor oligonucleotide, and fluorescence intensity and cell visualization were enhanced by the design andapplication of two adjacent helpers. (csic.es)
  • Probe performance was tested in soil samples along a pH gradient,and counting results matched the expected in situ distributions. (csic.es)
  • Probe is critical to in situ hybridization, and a right probe can help you achieve your goals. (creative-diagnostics.com)
  • Not only the probe types but also the label of probe should you take into account when you choose a probe for in situ hybridization. (creative-diagnostics.com)
  • There are essentially four types of probe that can be used in performing in situ hybridization. (creative-diagnostics.com)
  • To "see" where the probe has bound within your cells or tissue section you must attach a label to the probe before hybridization. (creative-diagnostics.com)
  • For hybridization histochemistry, sample cells and tissues are usually treated to fix the target transcripts in place and to increase access of the probe. (wikipedia.org)
  • Clinical evaluation of two consecutive UroVysion fluorescence in situ hybridization tests to detect intravesical recurrence of bladder cancer: a prospective blinded comparative study in Japan. (urotoday.com)
  • We evaluated the use of UroVysion fluorescence in situ hybridization tests to detect the intravesical recurrence of bladder cancer during follow-up after a transurethral resection of bladder tumor (TURBT). (urotoday.com)
  • Therefore we improved our previously developed chip platform for another additional analysis method, the fluorescence in situ hybridization. (sigmaaldrich.com)
  • Analysis of the phylogenetic diversity of estrone-degrading bacteria in activated sewage sludge using microautoradiography-fluorescence in situ hybridization. (biomedsearch.com)
  • There was a single case of carcinoma in situ in the analysis that was grouped with the unknown category following the criteria previously used ( 15 ). (aacrjournals.org)
  • Genotypic characterization of a primary mucoepidermoid carcinoma of the parotid gland by cytogenetic, fluorescence in situ hybridization, and DNA ploidy analysis. (nih.gov)
  • Where two fluorochromes are being used in the same specimen, it is important that their fluorescence signals can be clearly distinguished (for example, different populations of dye-labeled axons or different immunolabels). (alpfmedical.info)
  • Advances in both immunohistochemical markers as well as fluorescence in situ hybridization and epigenetic markers have proven helpful. (frontiersin.org)
  • Hav M, Libbrecht L, Pattyn P, Peeters M, Praet M, Pauwels P. Mdm2 in colon carcinoma: an immunohistochemical and fluorescence in situ hybridization study. (ugent.be)
  • In situ hybridization as a tool to study numerical chromosome aberrations in solid bladder tumors. (currentprotocols.com)
  • In situ hybridization is a powerful technique for identifying specific mRNA species within individual cells in tissue sections, providing insights into physiological processes and disease pathogenesis. (wn.com)
  • Numerical chromosomal aberrations in thyroid tumors detected by double fluorescence in situ hybridization. (semanticscholar.org)
  • However, cystoscopy is invasive, is expensive, and has poor sensitivity in detecting flat urothelial lesions such as carcinoma in situ. (bookaride.net)
  • In this study, the specific oligonucleotide fluorescence in situ hybridization probeSAG357 was designed for this Thaumarchaeota group based on the available 16S rRNA gene sequencesin databases, and included the ammonia-oxidizing species Nitrosotalea devanaterra. (csic.es)
  • Fluorescence in-situ hybridisation detected 558 (9.5%) patients with chromosomal abnormalities. (hkmj.org)
  • In general, background fluorescence increases with time stored. (alpfmedical.info)
  • Cox proportional hazards regression was used to assess the relationship between fluorescence in situ hybridization results and tumor recurrence or progression. (urotoday.com)
  • For example, patients with a negative fluorescence in situ hybridization result at baseline, 6 weeks and 3 months demonstrated an 8.3% recurrence rate compared to 48.1% for those with a positive result at all 3 points. (urotoday.com)
  • Fluorescence in situ hybridization results can identify patients at risk for tumor recurrence and progression during bacillus Calmette-Guérin immunotherapy. (urotoday.com)
  • Edited by two leading basic and clinical investigators in the field, with state-of-the-art contributions from expert subspecialists, and featuring over 200 color illustrations, Introduction to Fluorescence In Situ Hybridization is an essential reference for clinicians and investigators in oncology, genetics, and cytometry. (waterstones.com)
  • Eyada TM, Botros SK, El Noshokaty EH, Nabil G (2016) Prevalence of Chromosome 8 Aneuploidy in Egyptian Acute Myeloid Leukemia Patients using Fluorescence in situ Hybridization. (omicsonline.org)
  • We initiated a prospective clinical trial to determine whether fluorescence in situ hybridization results during bacillus Calmette-Guérin immunotherapy can predict therapy failure. (urotoday.com)
  • In situ hybridization has been widely used for research applications, including clinical cytogenetics, gene mapping, tumor biology and studies of chromosome evolution. (creative-diagnostics.com)
  • RNA ISH (RNA in situ hybridization) is used to measure and localize RNAs (mRNAs, lncRNAs, and miRNAs) within tissue sections, cells, whole mounts, and circulating tumor cells (CTCs). (wn.com)
  • In addition, in-situ hybridization on tissue sections require that tissue slices be very thin, usually 3 µm to 7 µm in thickness. (wikipedia.org)
  • To study their prognostic impact and association with kidney cancer phenotype, a tissue microarray with 1,809 cancers was analyzed by fluorescence in situ hybridization for 8p21 copy numbers. (urotoday.com)