A phenomenon manifested by an agent or substance adhering to or being adsorbed on the surface of a red blood cell, as tuberculin can be adsorbed on red blood cells under certain conditions. (Stedman, 25th ed)
Techniques for removal by adsorption and subsequent elution of a specific antibody or antigen using an immunosorbent containing the homologous antigen or antibody.
An immunoassay utilizing an antibody labeled with an enzyme marker such as horseradish peroxidase. While either the enzyme or the antibody is bound to an immunosorbent substrate, they both retain their biologic activity; the change in enzyme activity as a result of the enzyme-antibody-antigen reaction is proportional to the concentration of the antigen and can be measured spectrophotometrically or with the naked eye. Many variations of the method have been developed.
A species of bacteria that causes ANTHRAX in humans and animals.
Vaccines or candidate vaccines used to prevent ANTHRAX.
An acute infection caused by the spore-forming bacteria BACILLUS ANTHRACIS. It commonly affects hoofed animals such as sheep and goats. Infection in humans often involves the skin (cutaneous anthrax), the lungs (inhalation anthrax), or the gastrointestinal tract. Anthrax is not contagious and can be treated with antibiotics.
Small uniformly-sized spherical particles, of micrometer dimensions, frequently labeled with radioisotopes or various reagents acting as tags or markers.
A technique using antibodies for identifying or quantifying a substance. Usually the substance being studied serves as antigen both in antibody production and in measurement of antibody by the test substance.
Substances elaborated by bacteria that have antigenic activity.
The branch of medicine concerned with diseases, mainly of parasitic origin, common in tropical and subtropical regions.
A cell-separation technique where magnetizable microspheres or beads are first coated with monoclonal antibody, allowed to search and bind to target cells, and are then selectively removed when passed through a magnetic field. Among other applications, the technique is commonly used to remove tumor cells from the marrow (BONE MARROW PURGING) of patients who are to undergo autologous bone marrow transplantation.
Cytoplasm stored in an egg that contains nutritional reserves for the developing embryo. It is rich in polysaccharides, lipids, and proteins.
Infection with TREMATODA of the genus PARAGONIMUS.
A species of trematode blood flukes belonging to the family Schistosomatidae whose distribution is confined to areas of the Far East. The intermediate host is a snail. It occurs in man and other mammals.
Infection of the biliary passages with CLONORCHIS SINENSIS, also called Opisthorchis sinensis. It may lead to inflammation of the biliary tract, proliferation of biliary epithelium, progressive portal fibrosis, and sometimes bile duct carcinoma. Extension to the liver may lead to fatty changes and cirrhosis. (From Dorland, 27th ed)
The medical science concerned with the prevention, diagnosis, and treatment of diseases in animals.
Discussion of lists of works, documents or other publications, usually with some relationship between them, e.g., by a given author, on a given subject, or published in a given place, and differing from a catalog in that its contents are restricted to holdings of a single collection, library, or group of libraries. (from The ALA Glossary of Library and Information Science, 1983)
A list of works, documents, and other publications on medical subjects and topics of interest to the field of medicine.
Use for general articles concerning veterinary medical education.
Educational institutions for individuals specializing in the field of veterinary medicine.
A disorder characterized by the presence of ANEMIA, abnormally large red blood cells (megalocytes or macrocytes), and MEGALOBLASTS.
Red blood cell precursors, corresponding to ERYTHROBLASTS, that are larger than normal, usually resulting from a FOLIC ACID DEFICIENCY or VITAMIN B 12 DEFICIENCY.
Anemia characterized by larger than normal erythrocytes, increased mean corpuscular volume (MCV) and increased mean corpuscular hemoglobin (MCH).
A nutritional condition produced by a deficiency of VITAMIN B 12 in the diet, characterized by megaloblastic anemia. Since vitamin B 12 is not present in plants, humans have obtained their supply from animal products, from multivitamin supplements in the form of pills, and as additives to food preparations. A wide variety of neuropsychiatric abnormalities is also seen in vitamin B 12 deficiency and appears to be due to an undefined defect involving myelin synthesis. (From Cecil Textbook of Medicine, 19th ed, p848)
A nutritional condition produced by a deficiency of FOLIC ACID in the diet. Many plant and animal tissues contain folic acid, abundant in green leafy vegetables, yeast, liver, and mushrooms but destroyed by long-term cooking. Alcohol interferes with its intermediate metabolism and absorption. Folic acid deficiency may develop in long-term anticonvulsant therapy or with use of oral contraceptives. This deficiency causes anemia, macrocytic anemia, and megaloblastic anemia. It is indistinguishable from vitamin B 12 deficiency in peripheral blood and bone marrow findings, but the neurologic lesions seen in B 12 deficiency do not occur. (Merck Manual, 16th ed)
A megaloblastic anemia occurring in children but more commonly in later life, characterized by histamine-fast achlorhydria, in which the laboratory and clinical manifestations are based on malabsorption of vitamin B 12 due to a failure of the gastric mucosa to secrete adequate and potent intrinsic factor. (Dorland, 27th ed)
A cobalt-containing coordination compound produced by intestinal micro-organisms and found also in soil and water. Higher plants do not concentrate vitamin B 12 from the soil and so are a poor source of the substance as compared with animal tissues. INTRINSIC FACTOR is important for the assimilation of vitamin B 12.
Polymeric materials (usually organic) of large molecular weight which can be shaped by flow. Plastic usually refers to the final product with fillers, plasticizers, pigments, and stabilizers included (versus the resin, the homogeneous polymeric starting material). (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)
Forms to which substances are incorporated to improve the delivery and the effectiveness of drugs. Drug carriers are used in drug-delivery systems such as the controlled-release technology to prolong in vivo drug actions, decrease drug metabolism, and reduce drug toxicity. Carriers are also used in designs to increase the effectiveness of drug delivery to the target sites of pharmacological actions. Liposomes, albumin microspheres, soluble synthetic polymers, DNA complexes, protein-drug conjugates, and carrier erythrocytes among others have been employed as biodegradable drug carriers.
Exclusive legal rights or privileges applied to inventions, plants, etc.
Substances used for the detection, identification, analysis, etc. of chemical, biological, or pathologic processes or conditions. Indicators are substances that change in physical appearance, e.g., color, at or approaching the endpoint of a chemical titration, e.g., on the passage between acidity and alkalinity. Reagents are substances used for the detection or determination of another substance by chemical or microscopical means, especially analysis. Types of reagents are precipitants, solvents, oxidizers, reducers, fluxes, and colorimetric reagents. (From Grant & Hackh's Chemical Dictionary, 5th ed, p301, p499)
Condition of having pores or open spaces. This often refers to bones, bone implants, or bone cements, but can refer to the porous state of any solid substance.
Method of tissue preparation in which the tissue specimen is frozen and then dehydrated at low temperature in a high vacuum. This method is also used for dehydrating pharmaceutical and food products.
Removal of moisture from a substance (chemical, food, tissue, etc.).
The property of emitting radiation while being irradiated. The radiation emitted is usually of longer wavelength than that incident or absorbed, e.g., a substance can be irradiated with invisible radiation and emit visible light. X-ray fluorescence is used in diagnosis.
Measurement of the intensity and quality of fluorescence.
Antibodies that are chemically bound to a substrate material which renders their location fixed.
Binary classification measures to assess test results. Sensitivity or recall rate is the proportion of true positives. Specificity is the probability of correctly determining the absence of a condition. (From Last, Dictionary of Epidemiology, 2d ed)

Steroid regulation of retinol-binding protein in the ovine oviduct. (1/1855)

Two studies were conducted to identify retinol-binding protein (RBP) expression in the ovine oviduct and to determine the role of ovarian steroids in its regulation. Ewes were salpingectomized on Days 1, 5, or 10 of their respective estrous cycles, and oviducts were homogenized for RNA analysis, fixed for immunocytochemistry (ICC), or cultured for 24 h for protein analysis. ICC localized RBP to the epithelium of all oviducts. RBP synthesis was demonstrated by immunoprecipitation of radiolabeled RBP from the medium of oviductal explant cultures. Explant culture medium from oviducts harvested on Day 1 contained significantly more RBP than medium from oviducts collected on Days 5 or 10. Slot-blot analysis demonstrated that steady-state RBP mRNA levels were significantly higher on Day 1 than Day 5 or 10. In the second experiment, ovariectomized ewes were treated with estradiol-17beta (E2), progesterone (P4), E2+P4 (E2+P4), or vehicle control, and oviducts were analyzed as above. P4 alone or in combination with E2 significantly reduced steady-state RBP mRNA levels compared to those in E2-treated animals. Oviductal explants from E2- and E2+P4-treated animals released 3- to 5-fold more RBP into the medium than control and P4 treatments as determined by ELISA. RBP synthesis of metabolically labeled RBP was increased by E2 and E2+P4 treatments. This study demonstrates that P4 applied on an estradiol background negatively regulates RBP gene expression in the oviduct whereas estradiol appears to stimulate RBP synthesis and secretion.  (+info)

Serum sErbB1 and epidermal growth factor levels as tumor biomarkers in women with stage III or IV epithelial ovarian cancer. (2/1855)

Epithelial ovarian cancer (EOC) has a high mortality rate, which is due primarily to the fact that early clinical symptoms are vague and nonspecific; hence, this disease often goes undetected and untreated until in its advanced stages. Sensitive and reliable methods for detecting earlier stages of EOC are, therefore, urgently needed. Epidermal growth factor (EGF) is a ligand for EGF receptor (ErbB1); this receptor is the product of the c-erbB1 proto-oncogene. ErbB1 overexpression is common in human ovarian carcinoma-derived cell lines and tumors, in which overexpression is thought to play a critical role in tumor etiology and progression. Furthermore, ErbB1 overexpression is associated with disease recurrence and decreased patient survival. Recently, we have developed an acridinium-linked immunosorbent assay that detects a approximately 110-kDa soluble analogue of ErbB1, ie., sErbB1, in serum samples from healthy men and women (A. T. Baron, et al., J. Immunol. Methods, 219: 23-43, 1998). Here, we demonstrate that serum p110 sErbB1 levels are significantly lower in EOC patients with stage III or IV disease prior to (P < 0.0001) and shortly after (P < 0.0001) cytoreductive staging laparotomy than in healthy women of similar ages, whereas EGF levels are significantly higher than those of age-matched healthy women only in serum samples collected shortly after tumor debulking surgery (P < 0.0001). We observe that the preoperative serum sErbB1 concentration range of advanced stage EOC patients barely overlaps with the serum sErbB1 concentration range of healthy women. In addition, we show that serum sErbB1 and EGF levels changed temporally for some EOC patients who were surgically debulked of tumor and who provided a second serum sample during the course of combination chemotherapy. Finally, we observe a significant positive association between sErbB1 and EGF levels only in serum samples of EOC patients collected prior to cytoreductive surgery (correlation coefficient = 0.61968; P = 0.0027). These data suggest that epithelial ovarian tumors concomitantly affect serum sErbB1 and EGF levels. In conclusion, these data indicate that serum sErbB1 and EGF (postoperative only) levels are significantly different between EOC patients and healthy women and that altered and/or changing serum sErbB1 and EGF levels may provide important diagnostic and/or prognostic information useful for the management of patients with EOC.  (+info)

Differential serodiagnosis for cystic and alveolar echinococcosis using fractions of Echinococcus granulosus cyst fluid (antigen B) and E. multilocularis protoscolex (EM18). (3/1855)

Echinococcus granulosus cyst fluid and E. multilocularis protoscolex extract were fractionated by a single step of preparative isoelectric focusing, resulting in an antigen B-rich fraction (8-kD) and an Em18-rich fraction, respectively. The usefulness of both fractions for differential serodiagnosis of cystic (CE) and alveolar (AE) echinococcosis was evaluated by a large-scale immunoblot analysis on a battery of 354 serum samples. These included 66 from AE patients originating from four different endemic areas, 173 from CE patients originating from seven different endemic areas, 71 from patients with other parasitic diseases, 15 from patients with hepatomas, and 29 from healthy individuals. In an immunoblot with the antigen B-rich fraction, 92% (158 of 173) of the CE sera as well as 79% (52 of 66) of the AE sera reacted with the 8-kD subunit. No cross-reactivity occurred with any sera from patients with cysticercosis, other parasitic diseases, or with hepatomas, or from healthy controls. In an immunoblot with the Em18-rich fraction, all but two sera from AE patients (64 of 66, 97%) recognized Em18, and only nine of 34 CE sera from China reacted with it. All other (139) CE sera from six other countries were negative as were all (115) other non-echinococcosis sera. These findings indicate that antigen B (8-kD) is not species-specific for E. granulosus but is genus-specific for Echinococcus, and that the Em18 antigen is a reliable serologic marker for species-specific differentiation of AE from CE.  (+info)

Detection of haptoglobin in the high-density lipoprotein and the very high-density lipoprotein fractions from sera of calves with experimental pneumonia and cows with naturally occurring fatty liver. (4/1855)

In addition to the lipoprotein-deficient d > 1.25 fraction, haptoglobin was detected in the high-density lipoprotein (HDL) and the very high-density lipoprotein (VHDL) fractions from sera of calves with experimental pneumonia and cows with naturally occurring fatty liver. It was not found in the chylomicrons, very low-density lipoprotein and low-density lipoprotein fractions. Washing of the HDL fraction did not decrease the haptoglobin concentration. Transferrin and immunoglobulin G were immunoblotted to examine the possibility of contamination of the lipoprotein fractions by the d > 1.25 fraction. The two serum proteins were detected only in the d > 1.25 fraction, not in any lipoprotein fractions. The distribution pattern of haptoglobin in the lipoprotein fractions was distinct from that of serum albumin. Concentrations of haptoglobin in the HDL fractions from pneumonic sera were largely proportional to those in whole sera. Cholesteryl ester concentrations were decreased in sera from calves with pneumonia, as in cows with fatty liver. A protein immunologically related to hemoglobin was also detected in particular in the VHDL fractions from sera of both groups. These results suggest that haptoglobin or a complex with the hemoglobin-like protein may have a role or roles related to the lipid metabolism.  (+info)

Mutational analysis of apolipoprotein B mRNA editing enzyme (APOBEC1). structure-function relationships of RNA editing and dimerization. (5/1855)

APOBEC1 is the catalytic subunit of an enzyme complex that mediates apolipoprotein (apo) B mRNA editing. It dimerizes in vitro and requires complementation factor(s) for its editing activity. We have performed a systematic analysis of the structure-functional relationship of APOBEC1 by targeted mutagenesis of various sequence motifs within the protein. Using in vitro RNA editing assay, we found that basic amino acid clusters at the amino-terminal region R15R16R17 and R33K34, are essential for apoB mRNA editing. Mutation of R15R16R17 to K15K16K17 and mutation of R33K34 simultaneously to A33A34 almost completely abolished in vitro editing activity. The carboxy-terminal region of APOBEC1 contains a leucine-rich motif. Deletion analysis of this region indicates that residues 181 to 210 are important for in vitro apoB mRNA editing. Single amino acid substitutions demonstrate that L182, I185, and L189 are important residues required for normal editing function. Furthermore, the double mutant P190A/P191A also lost >90% of editing activity which suggests that a beta turn in this region of the molecule may be essential for proper functioning of APOBEC1. It was suggested that dimerization of APOBEC1 creates an active structure for deamination of apoB mRNA. When we examined the dimerization potential of truncated APOBEC1s using both amino and carboxy termini deletion mutants, we found that amino-terminal deletions up to residue A117 did not impair dimerization activity whereas carboxy-terminal deletions showed diminished dimerization. The systematic and extensive mutagenesis experiments in this study provide information on the role of various sequence motifs identified in APOBEC1 in enzyme catalysis and dimerization.  (+info)

Thyroid hormone induces activation of mitogen-activated protein kinase in cultured cells. (6/1855)

Thyroid hormone [L-thyroxine (T4)] rapidly induced phosphorylation and nuclear translocation (activation) of mitogen-activated protein kinase (MAPK) in HeLa and CV-1 cells in the absence of cytokine or growth factor. A pertussis toxin-sensitive and guanosine 5'-O-(3-thiotriphosphate)-sensitive cell surface mechanism responsive to T4 and agarose-T4, suggesting a G protein-coupled receptor, was implicated. Cells depleted of MAPK or treated with MAPK pathway inhibitors showed reduced activation of MAPK and of the signal transducer and activator of transcription STAT1alpha by T4; they also showed reduced T4 potentiation of the antiviral action of interferon-gamma (IFN-gamma). T4 treatment caused tyrosine-phosphorylated MAPK-STAT1alpha nuclear complex formation and enhanced Ser-727 phosphorylation of STAT1alpha, in the presence or absence of IFN-gamma. STAT1alpha-deficient cells transfected with STAT1alpha containing an alanine-for-serine substitution at residue 727 (STAT1alphaA727) showed minimal T4-stimulated STAT1alpha activation. IFN-gamma induced the antiviral state in cells containing wild-type STAT1alpha (STAT1alphawt) or STAT1alphaA727; T4 potentiated IFN-gamma action in STAT1alphawt cells but not in STAT1alphaA727 cells. T4-directed STAT1alpha Ser-727 phosphorylation is MAPK mediated and results in potentiated STAT1alpha activation and enhanced IFN-gamma activity.  (+info)

Transferrin stimulates iron absorption, exocytosis, and secretion in cultured intestinal cells. (7/1855)

The cellular mechanism by which basolateral transferrin (Tf) produces an increase in apical-to-basolateral Fe flux in Caco-2 cells was analyzed. After a pulse of 59Fe from the apical medium, three types of basolateral 59Fe efflux were found: a 59Fe efflux that was independent of the presence of Tf in the basolateral medium, a 59Fe efflux in which 59Fe left the cell bound to Tf, and a Tf-dependent 59Fe efflux in which 59Fe came off the cell not bound to Tf. Furthermore, addition of Tf to the basolateral medium doubled the exocytosis rate of Tf and increased the secretion of apolipoprotein A, a basolateral secretion marker. Both apotransferrin and Fe-containing Tf produced similar increases in 59Fe efflux, Tf exocytosis, and apolipoprotein A secretion. The Ca2+ channel inhibitor SKF-96365 inhibited both the Tf-mediated increase in transepithelial Fe transport and the secretion of apolipoprotein A. Thus the activation of transepithelial Fe transport by Tf seems to be mediated by Ca2+ entry into the cells.  (+info)

Involvement of protein serine and threonine phosphorylation in human sperm capacitation. (8/1855)

The involvement of serine and threonine phosphorylation in human sperm capacitation was investigated. Anti-phosphoserine monoclonal antibody (mAb) recognized six protein bands in the 43-55-kDa, 94 +/- 2-kDa, 110-kDa, and 190-kDa molecular regions, in addition to a faint band each in the 18-kDa and 35-kDa regions. Anti-phosphothreonine mAb recognized protein bands in six similar regions, except that the 18-kDa, 35-kDa, and 94 +/- 2-kDa protein bands were sharper and thicker, and an additional band was observed in the 110-kDa molecular region. In the 43-55-kDa molecular region, there was a well-characterized glycoprotein, designated fertilization antigen, that showed a further increase in serine/threonine phosphorylation after exposure to solubilized human zona pellucida. In a cell-free in vitro kinase assay carried out on beads or in solution, four to eight proteins belonging to similar molecular regions, namely 20 +/- 2 kDa, 43-55 kDa, 94 +/- 2 kDa, and 110 +/- 10 kDa, as well as in 80 +/- 4 and 210 +/- 10 kDa regions, were phosphorylated at dual residues (serine/tyrosine and threonine/tyrosine). Capacitation increased the intensity of serine/threonine phosphorylation per sperm cell, increased the number of sperm cells that were phosphorylated, and induced a subcellular shift in the serine/threonine-specific fluorescence. These findings indicate that protein serine/threonine phosphorylation is involved and may have a physiological role in sperm capacitation.  (+info)

Whilst we welcome the acknowledgement by the Foreign Minister that Amnesty International are regarded as a credible global organisation, his assertion that we will be able to conduct activities freely is a bold claim in light of the fact that both the Director and the Chair of Amnesty International Turkey are languishing behind bars alongside seven other human rights activists.. The absurd case against Idil Eser is not based on any nefarious activities but instead rests entirely upon the legitimate work of Amnesty International. If this work is criminalised, it is hard to see how the organisation - or indeed the wider human rights movement in Turkey - can continue to function.. Equally, the unfounded allegation that has landed Taner Kiliç in jail - that he downloaded and used Bylock on his phone - is false and we will show it. The vital work done by Amnesty International and the people who undertake that work cannot be separated. They are indivisible and we will fight to secure their ...
Summary The glycoproteins of cells infected with herpes simplex virus 1 or 2 (HSV-1 or HSV-2) were solubilized with non-ionic detergent NP-40 and electrophoresed in acrylamide gels. At least 12 bands containing virus glycoproteins were formed. The HSV-1 glycoproteins correspond in electrophoretic mobility to structural proteins in the HSV virions and to the virus proteins in plasma membranes of infected cells. All virus glycoproteins were bound to immunoadsorbent columns made by cross-linking either homologous or heterologous antibody. Immunoadsorbent columns readily discriminated between host and virus glycoproteins. The differentiation between HSV-1 and HSV-2 glycoproteins varied; the degree of specificity of the immunoadsorbent gels was related to the specificity exhibited by the antisera in neutralization tests. All immunoadsorbent antibody gels tested bound homologous glycoproteins in proportions similar to those in the input extract. Immunoadsorbent antibody gels of high specificity bound
DESCRIPTION: Recent phase II clinical studies demonstrate that an antibody targeting proportion convertase subtilisin/kexin type 9 (PCSK9)-a target for the treatment of hypercholesterolemia and coronary heart disease-successfully and safely decreases amounts of low-density lipoprotein-cholesterol (LDL) in patients with hypercholesterolaemia undertaking statin treatment.1,2 However, to date, there are no reports of small molecules or peptides achieving the same effect. This application offers a mature fragment-based design methodology to create novel small molecule PCSK9 inhibitors that achieve a targeted activity in surface plasmon resonance direct and competition assays as well as in a functional assay, LDL uptake in hepatocyte cells. Such an alternativeapproach to this problem is compelling because high-throughput screening campaigns at large pharmaceutical companies have generally not yielded progressable hits for PCSK9. Using our recently described hot-spot mapping technique,3,6 we found ...
Increased jejunal immunoglobulin synthesis in patients with nontropical sprue as measured by a solid phase immunoadsorption technique. J Lab Clin Med. 1972 Jun; 79(6):1004-13 ...
An immobilized immunoadsorbent for use in immunoassay and radioimmunoassay procedures includes, in a preferred form, a mass of superficially porous refractory particles having a water insoluble polymer, such as dextran, bonded thereto. The polymer in turn has bonded to it, by covalent bonds, antibodies capable of binding a specific antigen thereto and stoichiometrically releasing the bound antigen by the use of a rinsing solution which does not adversely affect the binding of the antibody to the polymer. Preferably, each of the porous particles includes an impervious core which has joined thereto sufficient layers of microparticles to form an outer porous coating on the core. Methods of forming such an immunoadsorbent and for using the same and structure for supporting the same are also disclosed.
A patient with a metastatic colon carcinoma was treated by immunoadsorption (IA) therapy using heat-killed, formalin-stabilized protein A-containing Staphylococcus aureus Cowan I as the immunoadsorbent. The patient experienced both subjective and objective positive clinical response without undue morbidity. The patients response correlated well with laboratory findings of decreased concentrations of carcinoembryonic antigen (CEA), immunoglobulin G (IgG), immune complexes (IC) and histopathologic data. The patient underwent surgery following 15 IA treatments; she lived for eighteen months, post-treatment.
Heart failure due to coronary heart disease (CHD) remains one of the most frequent causes of death. Left-ventricular ejection fraction , 30% is associated with a 5-year mortality , 70%. Therefore, new strategies and therapies towards treatment of heart failure are needed.. Heart failure due to left ventricular dysfunction can develop in CHD beyond the area of myocardial infarction. Some of these patients develop myocardial autoantibodies, which have been shown to exert a negative inotropic effect. Their elimination by immunoadsorption has been shown to improve left ventricular function in dilatative cardiomyopathy. Immunoglobulins are substituted to minimize infection risk at a level, which has been shown not to effect cardiac function. This intervention might also ameliorate cardiac function in patients with heart failure due to other origins. This study therefore aims to evaluate the effect of immunoadsorption with subsequent immunoglobulin substitution. ...
Wariant genu SARS-CoV-2 E zmienia charakterystykę czułości analitycznej wykrywania wirusów przy użyciu komercyjnego testu RT-PCR Diagnostic assays for detecting SARS-CoV-2 are important for affected. Read More ...
The study covers current and future market trends, drivers, challenges, and opportunities in US therapeutic apheresis market. It breaks down the market into hematology and oncology, neurology, rheumatology, and other therapeutic areas. Therapeutic apheresis has become an essential element of the blood and blood components industry value chain. Hospitals are the primary users of apheresis equipment and solutions; other users include blood banks and dialysis centers. End users include therapeutic apheresis nurses, referring physicians, hematologists, oncologists, rheumatologists, neurologists, nephrologists, cardiologists, and lipidologists. The study covers standard plasmapheresis or plasma exchange, extracorporeal immunoadsorption, plateletpheresis, red cell exchange, leukapheresis, and lipidpheresis. The two broad product categories are devices and disposables. The study discusses key trends that are expected to impact the market, including more applications across different therapeutics segments and
The study covers current and future market trends, drivers, challenges, and opportunities in US therapeutic apheresis market. It breaks down the market into hematology and oncology, neurology, rheumatology, and other therapeutic areas. Therapeutic apheresis has become an essential element of the blood and blood components industry value chain. Hospitals are the primary users of apheresis equipment and solutions; other users include blood banks and dialysis centers. End users include therapeutic apheresis nurses, referring physicians, hematologists, oncologists, rheumatologists, neurologists, nephrologists, cardiologists, and lipidologists. The study covers standard plasmapheresis or plasma exchange, extracorporeal immunoadsorption, plateletpheresis, red cell exchange, leukapheresis, and lipidpheresis. The two broad product categories are devices and disposables. The study discusses key trends that are expected to impact the market, including more applications across different therapeutics segments and
The human P450III family has been shown to be composed of at least four members, P450IIIA3 (HLp), P450IIIA4 (P450NF), P450IIIA5 (HLp3), and P450IIIA6 (HLp2). Due to the lack of probes that specifically recognize the individual members of this family, little is known about their relative expression. We prepared a form-specific antibody to P450IIIA5 by immunoabsorption of anti-P450IIIA5 IgG against Sepharose 4B upon which microsomes that did not contain P450IIIA5 or purified P450IIIA3 had been bound. Immunoblot analyses demonstrated that P450IIIA5 was expressed at detectable levels in only 19 of 66 (29%) human livers. The expression of P450IIIA5 was not influenced by the gender or medical history of the patients. When the expression of P450IIIA5 in different age groups was examined, it was observed that P450IIIA5 was detected in a statistically significantly higher percentage of children and adolescents (19 years old and under), as compared with the remaining population (8 of 17, 47%, versus 11 of ...
List of words make out of Nitrocellulose. All anagrams of Nitrocellulose. Words made after unscrambling Nitrocellulose. Scrabble Points. Puzzle Solver. Word Creation.
Immunoaffinity Column Rack [CR1] - Intended use: R-Biopharm Rhône has designed a simple and easy to use racking system for performing immunoaffinity column analysis. Inserts are also available for the rack.General information: With its solid, chemical resistant structure, RBRs variable position immunaffinity column rack gives users a stable foundation to handle multiple analysis. Holding up
Bacteria reactive to plaque toxin neutralizing monoclonal antibodies are related to the severity of gingivitis at the sampled site is an eagle-i resource of type Journal article at eagle-i Network Shared Resource Repository.
Barz D, Friedrich S, Schuller A, Rummler S.. Keywords: Antibody against angiotensin II-R1; Donor-specific-antibody; Eluate; Graft survival; Immunoadsorption Abstract. BACKGROUND:. The influence of ATR-1-autoantibodies on antibody mediated rejection (AMR) is still discussed controversially. Here we demonstrate some aspects as to diagnostics, treatment, clinical relevance and graft outcome.. METHODS:. A total of 27 transplant recipients (6 heart, 16 kidney, 3 lung and 2 multi-organ) suffering from AMR and a control group without transplant (8 pre-Tx, 1 pregnancy and 16 autoimmune and haematological diseases) were studied. In total, 290 IA eluates and the corresponding patient serum samples before and after immunoadsorption (IA) were analysed.. RESULTS:. ATR-1-and ETR-auto-antibodies (aAB) were found only in 4.5% of sera previous to IA treatment by using ELISA, but could be detected in 42% of IA eluates. AB with very high titres (,1:8 to 1:256) in the eluate were found more frequently in heart than ...
Extracorporeal blood correction (hemosorption, immunoadsorption, plasmapheresis) in Henoch-Schonlein purpura (costs for program #179319) ✔ DKD HELIOS Clinic Wiesbaden ✔ Department of Bone Marrow Transplant ✔ BookingHealth.com
Extracorporeal blood correction (hemosorption, immunoadsorption, plasmapheresis) in Henoch-Schonlein purpura (costs for program #144113) ✔ Academic Hospital Harlaching ✔ Department of Hematology, Oncology and Palliative Medicine ✔ BookingHealth.com
Importance of herbal medicines have recently increased owing to rising interest in their health benefits. However, medicinal plant extracts are complex mixtures of phytochemicals that act synergistically or additively on specific and/or multiple molecular and cellular targets. Thus, it is difficult to examine the actual pharmacological roles of active compounds in plant extracts. This review describes a new strategy for isolating target compounds from plant extracts using immunoaffinity columns coupled with monoclonal antibodies (mAbs) against natural compounds. Through one-step purification using mAb-coupled immunoaffinity columns, we succeeded in preparing a knockout (KO) extract, which contains all components except the target compound. Furthermore, we investigated the pharmacological effects of the KO extract to reveal the actual effects of a bioactive compound in the crude extract. This approach may help determine the potential function of target compounds in herbal medicines.
CRYOcheck ™ Factor Deficient Plasma consist of pools of citrated human normal plasmas which have been depleted into a coagulation factor by immunoadsorption, buffered with HEPES buffer, aliquoted and frozen rapidly.. ...
Monoclonal antibodies are a rapidly emerging group of drugs with the potential for treating diseases from arthritis to cancer. Now, a new technique called magnifection is set to drastically reduce the time and expense needed to make these molecules, ensuring that enough can be made to treat patients at a reasonable cost. Imagine reading the…
TY - JOUR. T1 - Partial purification of presynaptic plasma membrane by immunoadsorption. AU - Miljanich, G. P.. AU - Brasier, A. R.. AU - Kelly, R. B.. PY - 1982. Y1 - 1982. N2 - During transmitter release, synaptic vesicle membrane is specifically inserted into the nerve terminal plasma membrane only at specialized sites or active zones. In an attempt to obtain a membrane fraction enriched in active zones, we have utilized the electric organ of the marine ray. From this organ, a fraction enriched in nerve terminals (synaptosomes) was prepared by conventional means. These synaptosomes were bound to microscopic beads by an antiserum to purified electric organ synaptic vesicles (anti-SV). The success of this immunoadsorption procedure was demonstrated by increased specific activities of bead-bound nerve terminal cytoplasmic markers and decreased specific activities of markers for contaminating membranes. To obtain a presynaptic plasma membrane (PSPM) fraction, we lysed the bead-bound ...
Over the past few months, the public has learned about many treatments being used to combat COVID-19. An antiviral like remdesivir inhibits the virus from replicating in human cells. Convalescent plasma from the blood of donors who have recovered from COVID-19 may contain antibodies that suppress the virus and inflammation. Steroids like dexamethasone may modify and reduce the dangerous inflammatory damage to the lungs, thereby slowing respiratory failure.. The FDA issued emergency use authorization for Eli Lillys monoclonal antibody, called bamlanivimab, and Regeneron is waiting for FDAs green light for its antibody treatment. Monoclonal antibodies are particularly promising in therapy because they can neutralize the SARS-CoV-2 virus, which causes COVID-19, and block its ability to infect a cell. This might be a lifesaving intervention in people who are unable to mount a strong natural immune response to the virus - those over 65 or with existing conditions that make them more ...
Monoclonal antibodies (Mabs), produced in the laboratory, are derived from the antibodies that the human body produces in the natural course to fight intruders that threaten health. The discovery of these antibodies have radically transformed our understanding of how diseases progress, and how they can be more quickly, accurately, and cheaply diagnosed, and thus substantially expanded the horizons of treatment for more than 50 major ailments. The importance of Mabs can be estimated from the fact that today a majority of the most commercially-successful drugs are Mab-derived, and Mab-drugs are projected to clock sales of US$125 billion annually by 2020.. Mabs in Cancer Treatment Cancer is unarguably one of the fields where monoclonal antibodies have had the strongest impact. The biggest advantage of Mabs is that they are able to avoid the healthy cells while specifically targeting the cancer cells. This translates to the patient experiencing fewer side effects than conventional radiotherapy or ...
Specificity: hormone treated animals - Binding capacity determined by RIA : approx. 60 ng of clenbuterol per 400 l of resin column ...
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Objective: After detection of immunological abnormalities in dilated cardiomyopathy (DCM), including autoimmune reactivity against myocytes, attention has been focused on autoimmune mechanisms as potential key elements in DCM pathogenesis. DCM often appeared related to elevated autoantibody (AAB) levels against cardiac proteins including β1-adrenoreceptors. IgG reduction by unselective immunoadsorption (IA) i.e. extracorporeal IgG adsorption to levels at which β1-AABs become nearly undetectable, can be an efficient treatment. Nevertheless, there is controversy on whether β1-AAB removal by IA is indeed the major cause for cardiac improvement. We compared the results of unspecific IA and selective β1-AAB removal.. Methods: In 8/2000-1/2005 DCM patients with LVEF ≤ 30%; and evidence of serum β1-AABs underwent random selection for either unspecific or specific IA which was performed on 5 consecutive days with daily β1-AAB measurements. For unspecific (unselective) IA we used columns ...
Monoclonal antibodies are successful biologics in treating a variety of diseases, including the prevention or treatment of viral infections. CL184 is a 1:1 combination of two human monoclonal IgG1 antibodies (CR57 and ...
Monoclonal antibodies are substances produced in a lab that attach to certain proteins in the body (like a key in a lock). The antibodies can boost your bodys natural defenses against disease or can be used to kill cancer cells or slow the progress of a disease. Monoclonal antibodies are given through an intravenous...
Hoffmann, Frank; Kraft, Andrea; Heigl, Franz; Mauch, Erich; Koehler, Jürgen; Harms, Lutz; Kuempfel, Tania; Koehler, Wolfgang; Ehrlich, Sven; Bayas, Antonios; Weinmann-Menke, Julia; Beuker, Carolin; Henn, Karl-Heinz; Ayzenberg, Ilya; Ellrichmann, Gisa; Hellwig, Kerstin; Klingel, Reinhard; Fassbender, Cordula Marie; Fritz, Harald; Slowinski, Torsten; Weihprecht, Horst; Brand, Marcus; Stiegler, Thomas; Galle, Jan; Schimrigk, Sebastian (2018): Tryptophan immunoadsorption during pregnancy and breastfeeding in patients with acute relapse of multiple sclerosis and neuromyelitis optica. In: Therapeutic Advances in Neurological Disorders, Vol. 11 [PDF, 241kB] ...
"Enzyme-linked immunosorbent assay (ELISA)". British Society for Immunology. Mary Louise Turgeon (10 February 2015). Linne & ... These surveys are potentially the most direct and informative technique available to infer the dynamics of a population's ... Ringsrud's Clinical Laboratory Science - E-Book: The Basics and Routine Techniques. Elsevier Health Sciences. pp. 586-95, 543, ...
Other staining techniques include acid-fast staining, which will stain the oocysts red. One type of acid-fast stain is the ... Enzyme-linked immunosorbent assay (ELISA) also detects antigens. Polymerase chain reaction (PCR) is another way to diagnose ... There are several techniques to concentrate either the stool sample or the oocysts. The modified formalin-ethyl acetate (FEA) ... This can be done with direct fluorescent antibody (DFA) techniques. It can also be achieved through indirect immunofluorescence ...
The technique was developed by Kary Mullis in 1983 while working for the Cetus Corporation. Mullis would go on to win the Nobel ... Enzyme linked immunosorbent assays are used most commonly as diagnostic tests to detect HIV antibodies in blood samples to test ... The same technique can be used to create a gene insertion or deletion. Often, an antibiotic resistant gene is inserted along ... Techniques are used to affect the amino acid sequence of the protein (site-directed mutagenesis), the folding and conformation ...
It soon became the primary technique employed in equine drug testing. ELISA is an acronym for Enzyme Linked Immuno Sorbent ... Some drug testing laboratories have moved toward using the more specific mass spectrometric techniques as their primary ... equine drug testing was for all practical purposes dependent on a screening technique called thin layer chromatography (TLC). ... screening and testing technique. Zero Tolerance Testing Zero tolerance testing is not testing down to "zero" molecules, which ...
Immunochemical techniques include: enzyme-linked immunosorbent assay, immunoblotting (e.g., Western blot assay), precipitation ...
... the immunosorbent must be prepared. A technique to accomplish this was published by Wide and Jerker Porath in 1966. In 1971, ... The technique essentially requires any ligating reagent that can be immobilized on the solid phase along with a detection ... This is in contrast to "dry lab" techniques that use dry strips. Even if the sample is liquid (e.g., a measured small drop), ... Laboratory Techniques in Biochemistry and Molecular Biology. 25. pp. 1-6. ISBN 9780080887364. Hsieh, Y.-H.P.; Rao, Q. (2017). ...
Proceedings: ELISA, enzyme-linked immunosorbent assay-a new technique for sero-diagnosis of trichinosis. Parasitology 1974;69: ... The ELISA technique was conceptualized and developed by two Swedish scientists: Peter Perlmann (principal investigator) and Eva ... Enzyme-linked immunosorbent assay (ELISA) uses antibodies to detect proteins and other different immunogens. ... Stockholm : Univ.,Stockholm, 1975; LIBRIS record Lequin RM., Enzyme Immunoassay (EIA)/Enzyme-Linked Immunosorbent Assay (ELISA ...
Techniques include western blot, immunohistochemical staining, enzyme linked immunosorbent assay (ELISA) or mass spectrometry. ... There are several specific techniques and protocols that use antibodies for protein detection. The enzyme-linked immunosorbent ... A number of techniques allow to test for proteins produced during a particular disease, which helps to diagnose the disease ... Using bioinformatics techniques, researchers are capable of faster analysis and data storage. A good place to find lists of ...
Blood samples can also be tested using antibody reaction of enzyme-linked immunosorbent assay. Taenia solium deeply affects ... In secondary hosting, imaging techniques such as computed tomography and nuclear magnetic resonance are often employed. ...
... a semiquantitative stool examination technique. Other methods that can be used are enzyme-linked immunosorbent assay, ... the egg output can be quantified by using the Kato-Katz technique (20 to 50 mg of fecal material) or the Ritchie technique. ... The diagnosis is improved through the use of the Kato technique, ...
Molecular diagnostic techniques for phytoplasma detection began to emerge in the 1980s and included enzyme-linked immunosorbent ... More recent techniques allow infection levels to be assessed. Both quantitative PCR and bioimaging can effectively quantify ... Thus, classical diagnostic techniques, including symptom observation were used. Ultrathin sections of phloem tissue from plants ... In the early 1990s, polymerase chain reaction (PCR)-based techniques were developed: these are far more sensitive than ELISAs, ...
In each of these techniques, the antibodies for the protein of interest are added and the presence of the specific protein is ... Western blot (immunoblot assay) and enzyme-linked immunosorbent assay (ELISA) are two other methods used to detect proteins (or ...
The development of these diagnostic techniques has led to an increase in the speed of diagnosis, allowing for it to occur in- ... may be used to confirm the infection include serological methods such as lateral-flow devices and enzyme-linked immunosorbent ...
The enzyme-linked immunosorbent assay (ELISA) technique used for the analysis of YTXs is a recently developed method by Briggs ... The sensitivity of these techniques can, however, be poor due to the low molar absorptivity of the analytes. The technique ... This technique is also beneficial because it gives good levels of YTX recovery - ranging from 40-50%. A range of analytical ... The techniques used for YTX analysis include: CE with ultraviolet (UV) detection and CE coupled to mass spectrometry (MS). CEUV ...
Enzyme-linked immunosorbent assay (ELISA) screening of crops and seed potatoes replaced visual inspection in the early 1970s. ... A single nucleotide polymorphism-based technique for specific characterization of YO and YN isolates of Potato virus Y (PVY). J ... This means that quantitative PCR can be implemented as a high-throughput technique for sample screening. Quantitative PCR has ... Gugerli, P. and Gehriger, W. (1980). Enzyme-linked immunosorbent assay (ELISA) for the detection of potato leafroll virus and ...
Botulinum toxin can be detected by a variety of techniques, including enzyme-linked immunosorbent assays (ELISAs), ... They promoted the improvement of fermentation techniques among local residents, and thus eliminated the disease. From 1985-2015 ...
... funnel technique after culture by the Harada-Mori filter paper technique after culture in agar plates Culture techniques are ... Carroll SM, Karthigasu KT, Grove DI (1981). "Serodiagnosis of human strongyloidiasis by an enzyme-linked immunosorbent assay". ... Beal CB, Viens P, Grant RG, Hughes JM (March 1970). "A new technique for sampling duodenal contents: demonstration of upper ... The duodenal fluid can be examined using techniques such as the Enterotest string or duodenal aspiration. Larvae may be ...
It is a component of enhanced chemiluminescence enzyme-linked immunosorbent assay (ELISA) kits, used for the detection of ... Kricka, Larry J (1993). "Ultrasensitive immunoassay techniques". Clinical Biochemistry. 26 (5): 325-331. doi:10.1016/0009-9120( ...
The formalin-ether concentration technique (FECT) method of stool examination is most effective at diagnosing light cases of ... Serological methods that use enzyme-linked immunosorbent assay (ELISA) can help differentiate the eggs of C. sinensis from ...
Research on the viability of this new technique, as well as many others, as a means of monitoring the levels of rBST in dairy ... Dairy cattle do respond to the rBST antigen and have shown positive results in immunosorbent trials on milk samples. Tests ...
The enzyme-linked immunosorbent assay (ELISA) can be used as the technique to detect walnuts residues with great sensitivity ... Enzyme‐Linked Immunosorbent Assay technique containing high susceptibility and specificity is reliable method to investigate ... C and the time is set to 15 minutes for the current abstraction technique. The current abstraction technique can restrain the ... Three different techniques can be used in raw ground beef: the VIDAS ultraperformance E. coli test (ECPT UP), a noncommercial ...
Enzyme-linked immunosorbent analysis ELISA on particles filtered from the air. The original Fuchs, and the slightly modified ... Key characteristics of a technique that can be adapted to field use, as opposed to slow and labor-intensive methods such as ... One technique involves continual sampling of air through a nondispersive infrared analyzer. More complex instrumentation, such ... As with many branches of MASINT, specific techniques may overlap with the six major conceptual disciplines of MASINT defined by ...
Enzyme linked immunosorbent assay (ELISA) has become the most widely used technique for testing for anti-ENAs due to them being ... Three additional techniques, passive hemagglutination, enzyme linked immunosorbent assay (ELISA), and western blotting (WB), ... On top of that, false positive can occur and the disease specificity is lower than other techniques. While each technique has ... Due to limitations of gel-based techniques in disease sensitivity, other techniques have been explored in order to increase ...
Surface plasmon resonance is an example of technique that can detect binding between an unlabeled antibody and antigens. ... Immunoassays which employ enzymes are referred to as enzyme immunoassays (EIAs), of which enzyme-linked immunosorbent assays ( ... Immunoassays became considerably simpler to perform and more popular when techniques for chemically linked enzymes to ... Lequin R (2005). "Enzyme immunoassay (EIA)/enzyme-linked immunosorbent assay (ELISA)". Clin. Chem. 51 (12): 2415-8. doi:10.1373 ...
These may include: Enzyme-linked immunosorbent assay (ELISA) Enzyme multiplied immunoassay technique (EMIT) This article ...
Diagnosis of the oropouche infection is done through classic and molecular virology techniques. These include: Virus isolation ... tests and in-house-enzyme linked immunosorbent assay for total immunoglobulin, IgM, and IgG detection using convalescent sera ( ...
Enzyme-linked immunosorbent assay (ELISA) Transmission electron microscopy Virus isolation in cell culture In the 20th century ... include Full skin thickness biopsies Vesicular fluid if available Scabs Lymph node aspirates Lung and skin lesions Techniques ...
Many diagnostic techniques for torovirus infection in clinical specimens are now available such as hemagglutination (HA), ... enzyme-linked immunosorbent assay (ELISA), immune electron microscopy ,hemagglutination-inhibition tests (HA/HI), and nucleic ... It is also the reason that the molecular techniques are considered more promising than the serological assays. Pigs can be ... In 1984, torovirus like particles were detected with Electron Microscope (EM) technique in the human patients with ...
This technique is difficult to use on more than a single zinc finger at a time, so a multi-step processes that generated a ... is tested using a multitarget enzyme-linked immunosorbent assay (ELISA). The ZFPs are applied to microtitre wells coated with ... The techniques used in selection are similar to those described below except that the target oligonucleotide used in selection ... In order to keep the library size within reasonable limits, this technique is restricted to randomisation of only the key ...
... and enzyme-linked immunosorbent assay (ELISA). The name western blot is a play on the Southern blot, a technique for DNA ... The western blot technique was used during the 2014 FIFA World Cup in the anti-doping campaign for that event. In total, over ... Western blot technique uses three elements to achieve its task of separating a specific protein from a complex: separation by ... Further applications of the western blot technique include its use by the World Anti-Doping Agency (WADA). Blood doping is the ...
... and detecting proteins by enzyme-linked immunosorbent assay (ELISA) are methods best used in the early stages of the disease ... Education of caregivers in these techniques, and providing such barrier-separation supplies has been a priority of Doctors ...
IgE հակամարմինների հայտնաբերման լաբորատոր մեթոդները ներառում են իմունոֆերմենտային (enzyme-linked immunosorbent assay ELISA)[83] ... Revue Scientifique et Technique. 19 (1): 240-58. doi:10.20506/rst.19.1.1218. PMID 11189719. ...
The technique did have a 0.5-2.0% mortality rate, but that was considerably less than the 20-30% mortality rate of the disease ... Serologic tests and enzyme linked immunosorbent assays (ELISA), which measured Variola virus-specific immunoglobulin and ... Voltaire does not speculate on where the Circassians derived their technique from, though he reports that the Chinese have ... technique that were published in 1809, stated, "Had every practitioner been as uniformly successful in the disease as he was, ...
1994). "Enzyme-linked immunosorbent assay (ELISA)". Methods Mol Biol. 32: 461-466. doi:10.1385/0-89603-268-X:461. ISBN 0-89603- ... Rosetta Antibody is a novel antibody FV region structure prediction server, which incorporates sophisticated techniques to ...
ELISA (Enzyme-linked immunosorbent assay)[edit]. - quantitative analytical method that measures absorbance of color change from ... Analysis of foods and beverages : modern techniques. Charalambous, George, 1922-1994. Orlando: Academic Press. 1984. ISBN ...
Intense contact tracing and strict isolation techniques largely prevented further spread of the disease in the countries that ... and detecting proteins by enzyme-linked immunosorbent assay (ELISA) are methods best used in the early stages of the disease ... Education of caregivers in these techniques, and providing such barrier-separation supplies has been a priority of Doctors ...
The most commonly employed methods in medical laboratories include Enzyme-Linked Immunosorbent Assays, aka ELISA (among other ... Diagnostic testing includes molecular biology and genetic techniques which provide high accuracy and high sensitivity/ ...
Assessment of the A and B toxins by enzyme-linked immunosorbent assay (ELISA) for toxin A or B (or both) has a sensitivity of ... the differing terminology reflects the predominant techniques used for epidemiological typing. This strain is referred to as C ... effect observed with specific antisera is the practical gold standard for studies investigating new CDI diagnostic techniques.[ ...
Researchers in biochemistry use specific techniques native to biochemistry, but increasingly combine these with techniques and ... In fact, the enzyme-linked immunosorbent assay (ELISA), which uses antibodies, is one of the most sensitive tests modern ... These techniques allowed for the discovery and detailed analysis of many molecules and metabolic pathways of the cell, such as ... There has never been a hard-line among these disciplines in terms of content and technique. Today, the terms molecular biology ...
Benefits of this technique include the ability to perform genetic characterization, strain typing, and antiviral susceptibility ... These include both enzyme immunosorbent assays (EIA) and chromatographic immunoassays (CIA).[9][27] ... Common identification techniques include antigen testing, molecular testing, and viral culture.[9] ... when the viral burden may still be too low to be identified by traditional techniques. Because of its sensitivity, PCR can also ...
Medical staff should be trained and apply strict barrier nursing techniques (disposable face mask, gloves, goggles, and a gown ... that can be used for NP-capture enzyme-linked immunosorbent assay". Journal of Medical Virology. 76 (1): 111-118. doi:10.1002/ ... "Marburgvirus nucleoprotein-capture enzyme-linked immunosorbent assay using monoclonal antibodies to recombinant nucleoprotein: ...
Techniques such as computed tomography and magnetic resonance imaging made it possible to categorize mass of adipose tissue ... January 2004). "Enzyme-linked immunosorbent assay for circulating human resistin: resistin concentrations in normal subjects ... In the late 1980s and early 1990s insightful and powerful imaging techniques were discovered that would further help advance ...
The technique did have a 0.5-2.0% mortality rate, but that was considerably less than the 20-30% mortality rate of the disease ... Serologic tests and enzyme linked immunosorbent assays (ELISA), which measured Variola virus-specific immunoglobulin and ... Voltaire does not speculate on where the Circassians derived their technique from, though he reports that the Chinese have ... not knowing the value of this technique, and frightened of it, were left in danger." By 1800, approximately 2 million ...
Researchers in biochemistry use specific techniques native to biochemistry, but increasingly combine these with techniques and ... The enzyme-linked immunosorbent assay (ELISA), which uses antibodies, is one of the most sensitive tests modern medicine uses ... These techniques allowed for the discovery and detailed analysis of many molecules and metabolic pathways of the cell, such as ... with the development of new techniques such as chromatography, X-ray diffraction, dual polarisation interferometry, NMR ...
When coupled with diagnostic probes, this technique allows one to detect a small collection of molecules under very dilute ... structure generated via the WLA was designed to operate via a mechanism inspired by the Enzyme Linked ImmunoSorbent Assay ( ...
Diagnosis of leptospirosis is confirmed with tests such as enzyme-linked immunosorbent assay (ELISA) and polymerase chain ... This makes culture techniques useless for diagnostic purposes but is commonly used in research. ... Kidney tissue, using a silver staining technique, revealing the presence of Leptospira bacteria ...
Indirect ELISA and the western blotting technique are also used, but these techniques are not commonly used. During indirect ... Immunosorbent electron microscopy (ISEM) is the most commonly used detection method. ISEM is a process where tissue is ... Similar to indirect ELISA, the western blotting technique also uses two antibodies. The western blotting technique is used to ... To use this technique, first a small piece of tissue is taken off the grapevine. Cold phosphate buffered saline (PBS) is then ...
Using this technique combination, one can determine if a specific antigen is synthesized by a tissue or by a cell. Multiwell ... The enzyme-linked immunosorbent assay (ELISA) or Western blotting are two different ways that the purified antigen (or multiple ... Despite the different techniques used for non-radioactive assays, they require that ligands exhibit similar binding ... On the other hand, such techniques are also available to create receptor antagonists in order to prevent further cascades. Such ...
Various clinical scenarios and technique-related factors may impact the validity of results of the culture and sensitivity. ... Confirmation of Lyme disease is done through enzyme-linked immunosorbent assay (ELISA) followed by confirmation using Western ... In children, joint synovial fluid aspiration techniques aim at isolating the infectious organism by culture and sensitivity ...
Enzyme-linked immunosorbent assays (ELISAs) are easy to perform, provide a more objective interpretation of the results, and ... Development of the WB test over the years has benefited greatly in EPM diagnosis, however the WB technique is mainly a research ... 2003a;1:318-327 Enzyme-linked immunosorbent assays for detection of equine antibodies specific to Sarcocystis neurona surface ...
Laboratory methods to measure specific IgE antibodies for allergy testing include enzyme-linked immunosorbent assay (ELISA, or ... Revue Scientifique et Technique. 19 (1): 240-58. doi:10.20506/rst.19.1.1218. PMID 11189719. Carvalho EM, Bastos LS, Araújo MI ( ...
Real time PCR techniques using ISCfe1 insertion site is the most sensitive technique for diagnosis of Cfv-associated abortion. ... C. fetus can be diagnosed with polymerase chain reaction assays, enzyme linked immunosorbent assays and vaginal mucus ... "Evaluation of a monoclonal antibody-based enzyme-linked immunosorbent assay for detection of Campylobacter fetus in bovine ... Geographic Distribution and Recent Advances in Molecular Diagnostic Techniques". Reproduction in Domestic Animals. 45 (5): e221 ...
Immunologic enzyme-linked immunosorbent assay (ELISA) testing may be used for diagnosis. These tests are capable of a 90% ... current test of choice for diagnosis of giardiasis and provides increased sensitivity over more common microscopy techniques. A ... Rosenblatt JE, Sloan LM, Schneider SK (May-June 1993). "Evaluation of an enzyme-linked immunosorbent assay for the detection of ...
Generally these techniques require high sequence homology between parental sequences. These techniques are often used to ... followed by the identification of positive clones by enzyme linked immunosorbent assay. These selected phages are then ... This in vitro technique was one of the first techniques in the era of recombination. It begins with the digestion of homologous ... This technique results in the generation of tandem repeats of random fragments of the target gene via rolling circle ...
He is reported to have developed T3 and TSH hormone tests and the technique to contain cancerous cells using isotopes, ... is best known for his contributions to radioimmunoassays and enzyme-linked immunosorbent assays. He is credited with pioneering ...
A total of 37 serum samples from 27 cancer patients were tested by an enzyme-linked immunosorbent assay-inhibition technique ... Detection of Candida antigen in sera of patients with candidiasis by an enzyme-linked immunosorbent assay-inhibition technique. ... Detection of Candida antigen in sera of patients with candidiasis by an enzyme-linked immunosorbent assay-inhibition technique. ... Detection of Candida antigen in sera of patients with candidiasis by an enzyme-linked immunosorbent assay-inhibition technique. ...
Rotavirus, Polyacrylamide gel electrophoresis (PAGE) technique, Enzyme - linked immunosorbent assay (ELISA), Diahorrea ... PAGE and silver staining technique were performed as per the method of herring et al (5) and Merill et al (7). Briefly a 0.5ml ... PAGE and silver staining technique were performed as per the method of herring et al (5) and Merill et al (7). Briefly a 0.5ml ... The earliest technique used to diagnose Rotavirus infection was direct electron microscopy. The identification of virus is done ...
... and quantitative enzyme-linked immunosorbent assay (ELISA) for immunoglobulin G (IgG) antibodies to Bacillus anthracis ... Diagnostic-techniques; Antibody-response; Antigens; Bioassays; Biological-monitoring; Immunoglobulins; Enzyme-activity; ... Comparison of a multiplexed fluorescent covalent microsphere immunoassay and an enzyme-linked immunosorbent assay for ... We report a newly developed fluorescence covalent microbead immunosorbent assay (FCMIA) for B. anthracis PA which was Luminex ...
While commercial enzyme linked immunosorbent assays (ELISA) have been developed, they have not been fully validated. We ... Techniques. MIF test. Cpn species specific IgG and IgA antibodies were measured by our in house MIF test as described by Wang ... Frikha-Gargouri, O., Gdoura, R., Znazen, A. et al. Evaluation and optimization of a commercial enzyme linked immunosorbent ... Ngeh J, Gupta S, Goodbourn C: The reproducibility of an enzyme-linked immunosorbent assay for detection of Chlamydia pneumoniae ...
Abstract An enzyme-linked immunosorbent assay (ELISA) using a crude soluble antigen from adult Onchocerca volvulus was ... Techniques for Hemagglutination and Hemagglutination-Inhibition with Arthropod-Borne Viruses D. H. Clarke and J. Casals ... An enzyme-linked immunosorbent assay (ELISA) using a crude soluble antigen from adult Onchocerca volvulus was developed and ... Enzyme-Linked Immunosorbent Assay (Elisa) for Serodiagnosis of Guatemalan Onchocerciasis: Comparison with the Indirect ...
This IgY-based immunomagnetic bead enzyme-linked immunosorbent assay (IgY-IMB-ELISA) uses polyclonal IgY-coated magnetic beads ... Techniques for Hemagglutination and Hemagglutination-Inhibition with Arthropod-Borne Viruses D. H. Clarke and J. Casals ... The unreliability of the Kato-Katz technique limits its usefulness for evaluating S. mansoni infections. Trop Med Int Health 6 ... Comparison of a polymerase chain reaction and the Kato-Katz technique for diagnosing infection with Schistosoma mansoni . Am J ...
55 BIOLOGY AND MEDICINE, BASIC STUDIES; ARGENTINA; BABESIDAE; BLOOD SERUM; CATTLE; DIAGNOSIS; DIAGNOSTIC TECHNIQUES; ENZYME ... ETDEWEB / Search Results / Evaluation of an enzyme linked immunosorbent assay kit for the detection of Babesia bovis antibodies ... An enzyme linked immunosorbent assay (ELISA) for the detection of antibodies to Babesia bovis was evaluated by using sera of ... Evaluation of an enzyme linked immunosorbent assay kit for the detection of Babesia bovis antibodies in cattle in Argentina ...
... is a technique that can detect and quantify antigens or antibodies of interest in patient serum with high sensitivity and ... The enzyme-linked immunosorbent assay (ELISA) is a technique that can detect and quantify antigens or antibodies of interest in ... Techniques in Life Science and Biomedicine for the Non-Expert. Springer, Cham. * First Online 24 May 2018 ... Part of the Techniques in Life Science and Biomedicine for the Non-Expert book series (TLSBNE) ...
During the last decade enzyme-linked immunosorbent assay has been a technique of major interest to those engaged in ... Enzyme labelled immunosorbent assay techniques in foot and mouth disease virus research. J. Hyg. (London), 80: 391-399.Google ... During the last decade enzyme-linked immunosorbent assay has been a technique of major interest to those engaged in ... Detection and quantitation of foot and mouth disease virus by enzyme-labelled immunosorbent assay technique. J. Gen. Virol., 42 ...
Immunosorbent for diagnosis of epilepsy and group of risk US5344759A (en) * 1992-05-11. 1994-09-06. Her Majesty The Queen In ... Testing techniques. Patent Citations (3). * Cited by examiner, † Cited by third party. Publication number. Priority date. ... Immunosorbent for diagnosis of epilepsy and group of risk US5344759A (en) * 1992-05-11. 1994-09-06. Her Majesty The Queen In ... Single incubation immuno sorbent assay method using particle labels to detect test antigen specific antibodies in presence of ...
DNA recombinant techniques.Genes without signal peptides were amplified from the genomic DNA of L. interrogans serovar ... Evaluation of recombinant Leptospira antigen-based enzyme-linked immunosorbent assays for the serodiagnosis of leptospirosis. J ... Evaluation of two enzyme-linked immunosorbent assay methods for detection of immunoglobulin M antibodies in acute leptospirosis ... by enzyme-linked immunosorbent assays. The prevalences of total IgG antibodies against MPL17 and MPL21 were 38.5% and 21.2%, ...
The present invention relates to antigen-capture substrates useful for orienting capture antibodies for immunosorbent antigen ... Polymers can be coated onto the solid surface using a variety of techniques. In some embodiments the polymer is coated onto the ... including but not limited to enzyme-linked immunosorbent assay (ELISA), fluorescent-linked immunosorbent assay (FLISA), and ... Enzyme-linked immunosorbent assay (ELISA) is a well-known type of assay used for the detection of various antigens. The ...
1958) Techniques for hemagglutination and hemagglutination-inhibition with arthropod-borne viruses. Am. J. Trop. Med. Hyg. 7: ... Detection of Anti-Arboviral Immunoglobulin G by Using a Monoclonal Antibody-Based Capture Enzyme-Linked Immunosorbent Assay. ... Enzyme-linked immunosorbent assay (ELISA) provides a platform capable of integrating protocols for the wide variety of ... 1979) Detection of antibodies to alphaviruses by enzyme-linked immunosorbent assay. J. Clin. Microbiol. 10:583-585. ...
... fluorometric enzyme-linked immunosorbent assay (ELISA) is described for the detection and quantitation of metallothionein (MT ... Pesce, A.J., Krieger, N.J. and Michael, J.G. ( 1983 In: "Immunoenzymatic Techniques", S. Avrameas, P. Druet, R. Masseyeff and G ... Garvey J.S., Thomas D.G., Linton H.J. (1987) Enzyme-Linked Immunosorbent Assay (ELISA) for Metallothionein. In: Kägi J.H.R., ... Voller, A., Bidwell, D.E. and Bartlett, A. (1976). In: "Immunoenzymatic Assay Techniques", R. Malvano ed., Martinus Nijhoff, ...
Rapid enzyme-linked immunosorbent assay technique by ultrasound waves in a sonicator bath ... Ultrasound wave-mediated enzyme-linked immunosorbent assay technique. Pragya Sharma and Pradip Nahar. Analytica Chimica Acta ... a) Pragya Sharma, Pradip Nahar (2009) Ultrasound wave-mediated enzyme-linked immunosorbent assay technique. Analytica Chimica ... In fact any type of ELISA can be carried out by SELISA technique. SELISA method is sensitive and can detect lower concentration ...
Enzyme-linked Immunosorbent Assay) Data. Some Benefits of using Liquidator 96. ELISA is a common technique in modern biological ... METTLER TOLEDO supports and services your pipettes through their entire life-cycle, from good pipetting techniques to ...
Enzyme-linked Immunosorbent Assay) Data. Some Benefits of using Liquidator 96. ELISA is a common technique in modern biological ...
Serum Folate: This is also measured by ELISA technique. *Serum Methylmalonate and Homocysteine: In patients with cobalamin ... Serum Cobalamin: This is measured by ELISA (Enzyme Linked Immuno Sorbent Assay). Levels less than 100ng/L is diagnostic of a ...
Immunosorbent Techniques * Kinetics * Models, Molecular * Molecular Sequence Data * Plasmids * Protein Binding / drug effects ...
"Enzyme-linked immunosorbent assay (ELISA)". British Society for Immunology. Mary Louise Turgeon (10 February 2015). Linne & ... These surveys are potentially the most direct and informative technique available to infer the dynamics of a populations ... Ringsruds Clinical Laboratory Science - E-Book: The Basics and Routine Techniques. Elsevier Health Sciences. pp. 586-95, 543, ...
... bovis-infected animals not afforded by current diagnostic techniques. The present study describes assay development and field ... Development and evaluation of an enzyme-linked immunosorbent assay for use in the detection of bovine tuberculosis in cattle.. ... Development and Evaluation of an Enzyme-Linked Immunosorbent Assay for Use in the Detection of Bovine Tuberculosis in Cattle ... Development and Evaluation of an Enzyme-Linked Immunosorbent Assay for Use in the Detection of Bovine Tuberculosis in Cattle ...
in PIF, an indirect non-competitive enzyme-linked immunosorbent assay (INC-ELISA) was developed based on a novel immunoglobulin ... in PIF, an indirect non-competitive enzyme-linked immunosorbent assay (INC-ELISA) was developed based on a novel immunoglobulin ... During assay development, accuracy of the rapid detection method was confirmed by standard plate colony counting techniques. ... TABLE 2. Cross-reactivity of the developed indirect non-competitive enzyme-linked immunosorbent assay with non-Cronobacter ...
Other staining techniques include acid-fast staining, which will stain the oocysts red. One type of acid-fast stain is the ... Enzyme-linked immunosorbent assay (ELISA) also detects antigens. Polymerase chain reaction (PCR) is another way to diagnose ... There are several techniques to concentrate either the stool sample or the oocysts. The modified formalin-ethyl acetate (FEA) ... This can be done with direct fluorescent antibody (DFA) techniques. It can also be achieved through indirect immunofluorescence ...
Enzyme-linked immunosorbent assay (ELISA): A technique using antibodies for detecting specific proteins. Used to test for the ... particularly those techniques referred to as recombinant DNA techniques.. Genetically engineered organism (GEO): An organism ... Polymerase chain reaction (PCR): A technique used to create a large number of copies of a target DNA sequence of interest. One ... Field trial: A test of a new technique or variety, including biotech-derived varieties, done outside the laboratory but with ...
A sensitive technique (see immunoassay [1]) for accurately determining the amount of protein or other antigen in a given sample ... ELISA (enzyme-linked immunosorbent assay) A sensitive technique (see immunoassay) for accurately determining the amount of ... ELISA Enzyme‐linked immunosorbent assay; extremely sensitive and specific analytical technique using antibodies linked to an ...
... is a technique used to quantitatively detect an antigen within a sample such as peptides, proteins, antibodies and hormones, ... ELISA analysis and techniques. Enzyme-linked immunosorbent assay is a commonly used analytical technique performed in many ... ELISA (enzyme-linked immunosorbent assay) is a method used to quantitatively detect an antigen within a sample. An antigen is a ... Types of enzyme-linked immunosorbent assays (ELISAs). There are four major types of ELISAs: direct, indirect, competitive and ...
We have developed an enzyme-linked immunosorbent assay (ELISA) using three overlapping recombinant fragments of the human ... Although Western blot is a valuable serologic technique, its major limitation is that it is not quantitative. ELISA overcomes ... Enzyme-Linked Immunosorbent Assay and Serologic Responses to Pneumocystis jiroveci On This Page ... Enzyme-Linked Immunosorbent Assay and Serologic Responses to Pneumocystis jiroveci. Emerging Infectious Diseases. 2004;10(5): ...
Macroencapsulated immunosorbent assay technique. US4347312. 20 Mar 1980. 31 Aug 1982. Research Triangle Institute. Detection of ... "Immunochemical Techniques", Methods In Enzymology, vol. 73, pp. 339-348, (1981).. 65. Langone et al. "Immunochemical Techniques ... Such techniques are commonplace in the art, and form no part of the present invention. In the preferred embodiment, where the ... "Affinity Chromatography . . . Techniques", Elsevier Scientific Pub. Co., pp. 411-424, (1982).. 89. Van Hell, et al., "Particle ...
... techniques for the determination of immunoglobulin G to rickettsial lipopolysaccharides were developed. These techniques ... Use of enzyme-linked immunosorbent assay techniques with cross-reacting human sera in diagnosis of murine typhus and spotted ... Enzyme-linked immunosorbent assay (ELISA) techniques for the determination of immunoglobulin G to rickettsial ... These techniques provide a simple and convenient way to serodiagnose Mediterranean spotted fever and murine typhus with a ...
  • Recently, the Centers for Disease Control and Prevention reported an accurate, sensitive, specific, reproducible, and quantitative enzyme-linked immunosorbent assay (ELISA) for immunoglobulin G (IgG) antibodies to Bacillus anthracis protective antigen (PA) in human serum (C. P. Quinn, V. A. Semenova, C. M. Elie et al. (cdc.gov)
  • While commercial enzyme linked immunosorbent assays (ELISA) have been developed, they have not been fully validated. (biomedcentral.com)
  • Due to these drawbacks, several partially automated commercial enzyme linked immunosorbent assays (ELISA) have been developed. (biomedcentral.com)
  • An enzyme-linked immunosorbent assay (ELISA) using a crude soluble antigen from adult Onchocerca volvulus was developed and used as a serodiagnostic test for Guatemalan onchocerciasis. (ajtmh.org)
  • This IgY-based immunomagnetic bead enzyme-linked immunosorbent assay (IgY-IMB-ELISA) uses polyclonal IgY-coated magnetic beads as a capture antibody, and a monoclonal IgG as a detection antibody. (ajtmh.org)
  • An enzyme linked immunosorbent assay (ELISA) for the detection of antibodies to Babesia bovis was evaluated by using sera of 874 cattle carrying B. bovis antibodies, 700 sera of uninfected cattle, and 357 sera from calves from 16 herds subjected to different B. bovis inoculation rates. (osti.gov)
  • The enzyme-linked immunosorbent assay (ELISA) is a technique that can detect and quantify antigens or antibodies of interest in patient serum with high sensitivity and accuracy. (springer.com)
  • Engvall E, Perlmann P. Enzyme-linked immunosorbent assay (ELISA). (springer.com)
  • The enzymelinked immunosorbent assay (ELISA) for detection of antibodies to the bovine parvovirus. (springer.com)
  • Diagnosis of parasitic diseases by enzyme-linked immunosorbent assay (ELISA) with a modified micro-method: results for toxoplasmosis, amoebiasis, trichinosis, hydatidosis and aspergillosis. (springer.com)
  • Development of mycoplasma enzyme linked immunosorbent assay (Myco-ELISA) for the detection of antibody for mycoplasma infections in chickens. (springer.com)
  • Enzyme linked immunosorbent assay (ELISA) for the detection of antibodies to porcine cytomegalovirus. (springer.com)
  • Experiences with the enzyme linked immunosorbent assay (ELISA). (springer.com)
  • Diagnosis of bovine leukosis: ELISA, a new technique. (springer.com)
  • Rapid detection of antibodies of infectious bovine rhinotracheitis (IBR) virus by micro-enzyme linked immunosorbent assay (Micro-ELISA). (springer.com)
  • An enzyme linked immunosorbent assay (ELISA) for the detection of antibodies against Aujeszky's disease virus in pigs sera. (springer.com)
  • Enzyme-linked immunosorbent assay (ELISA) provides a platform capable of integrating protocols for the wide variety of arboviruses while offering well-documented advantages over more traditional serological methods such as the plaque-reduction neutralization test (PRNT), hemagglutination-inhibition test, and complement fixation test. (asm.org)
  • Garvey J.S., Thomas D.G., Linton H.J. (1987) Enzyme-Linked Immunosorbent Assay (ELISA) for Metallothionein. (springer.com)
  • In fact any type of ELISA can be carried out by SELISA technique. (scienceexchange.com)
  • This is also measured by ELISA technique. (medindia.net)
  • The enzyme-linked immunosorbent assay (ELISA) (/ɪˈlaɪzə/, /ˌiːˈlaɪzə/) is a commonly used analytical biochemistry assay, first described by Engvall and Perlmann in 1971. (wikipedia.org)
  • The present study describes assay development and field assessment of a new commercial enzyme-linked immunosorbent assay (ELISA) that detects antibody to M. bovis antigens MPB83 and MPB70 in infected cattle. (nih.gov)
  • in PIF, an indirect non-competitive enzyme-linked immunosorbent assay (INC-ELISA) was developed based on a novel immunoglobulin G (IgG), anti- Cronobacter IgG. (frontiersin.org)
  • ELISA (enzyme-linked immunosorbent assay) A sensitive technique (see immunoassay ) for accurately determining the amount of protein or other antigen in a given sample by means of an enzyme-catalysed colour change. (encyclopedia.com)
  • ELISA (enzyme-linked immunosorbent assay) is a method used to quantitatively detect an antigen within a sample. (moleculardevices.com)
  • We have developed an enzyme-linked immunosorbent assay (ELISA) using three overlapping recombinant fragments of the human Pneumocystis major surface glycoprotein (MsgA, MsgB, and MsgC) for analysis of antibody responses in HIV-positive patients and healthy blood donors. (cdc.gov)
  • Enzyme-linked immunosorbent assay (ELISA) techniques for the determination of immunoglobulin G to rickettsial lipopolysaccharides were developed. (naver.com)
  • So, Lisa, we're going to look at a different technique now called ELISA. (futurelearn.com)
  • Here, a simple and economical two-step enzyme-linked immunosorbent assay (ELISA) system is proposed for diagnosing NPC in high-risk populations, employing the peptide-based immunoglobulin A (IgA) EBNA1 plus viral capsid antigen p18 ELISA as an initial screening test and the IgA early antigen (EA) ELISA using a different set of EBV antigens as a confirmation test. (asm.org)
  • The immunodiagnostics is further segmented into enzyme-linked immunosorbent assay (ELISA), rapid tests, enzyme-linked immunospot (ELISPOT), radioimmunoassay (RIA), and western blot. (pharmiweb.com)
  • Cyclosporine is measured with high performance liquid chromatography (HPLC), fluorescence polarization immunoassay (FPIA), enzyme-multiplied-immunoassay techniques (EMIT), or liquid chromatography-tandem mass spectrometry (LC-MS/MS). Tacrolimus can be monitored with LC-MS/MS, enzyme-linked immunosorbent assay (ELISA), or microparticle enzyme immunoassay (MEIA). (ahrq.gov)
  • The sensitivity and specificity of the oral fluid-based immunoassay for HEV IgG (past HEV infection) andHEV IgA(recent HEV infection) antibodieswas calculated in reference toWantai's (BeijingWantai) serum-based HEV enzyme-linked immunosorbent assay (ELISA) kits for IgG and IgM antibodies, respectively. (cdc.gov)
  • RÉSUMÉ L'objectif de cette étude réalisée en Iraq était de définir la sensibilité et la spécificité d'un test ELISA commercial pour la détection de l'antigène de Giardia lamblia dans les selles. (who.int)
  • An enzyme-linked immunosorbent assay (ELISA) that detects excretory and secretory products of the organism is available now [3]. (who.int)
  • Se elaboraron dos sistemas de ELISA de captura de IgM (MAC-ELISA por sus siglas en inglés) para el diagnóstico de la infección aguda por el virus de (CHIKV) y dos métodos de ELISA de inhibición (MEI) para determinar el valor cuantitativo de los anticuerpos totales contra el CHIKV, en el Laboratorio Nacional de Virología de Nicaragua en 2014-2015, para lo cual se utilizaron anticuerpos monoclonales (AcMo) y sueros hiperinmunes. (scielosp.org)
  • La duración del procesamiento se redujo sustancialmente en comparación con el MAC-ELISA-CDC. (scielosp.org)
  • Con los sistemas de MAC-ELISA, se obtuvo una sensibilidad del 96,6% y del 97,4% y una especificidad del 98,8% y del 91,5% al utilizar AcMo y suero hiperinmune, respectivamente, en comparación con el método de los CDC. (scielosp.org)
  • Se estandarizaron dos sistemas de ELISA-MAC y dos de MEI y se comprobó que poseen la calidad adecuada para el diagnóstico y las investigaciones del chikungunya, con lo cual mejorará la eficiencia de la vigilancia epidemiológica en Nicaragua, el primer país centroamericano que produce sus propios reactivos para el diagnóstico serológico del CHIKV. (scielosp.org)
  • Most commercial clinical pathology laboratories will offer a range of ELISA-based techniques for a variety of purposes. (vetstream.com)
  • To identify the virus in animals, a nuclear related enzyme linked immunosorbent assay (ELISA) technology was developed. (iaea.org)
  • ELISA stands for Enzyme-Linked Immunosorbent Assay, the technique used to identify pathogens. (gardenguides.com)
  • and on various serological assays including precipitin tests, ELISA, blot immunoassays, immunosorbent electron microscopy and lateral flow immunoassays. (springer.com)
  • The West Nile Virus blood test is an enzyme-linked immunosorbent assay (ELISA) test. (brighthub.com)
  • ELISA is a biochemistry technique in which the antigen (virus) is affixed to the surface of the test area and washed over with an antibody, which sticks to the antigen. (brighthub.com)
  • Two common techniques used for protein quantification include Western blotting and enzyme-linked immunosorbent assay or ELISA. (news-medical.net)
  • The most common technique for the detection of allergen residues is the enzyme-linked immunosorbent assay (ELISA). (ift.org)
  • The recombinant proteins were used to evaluate their ability to bind to immunoglobulin G (IgG) (and IgG subclass) or IgM antibodies in serum samples from patients in the early and convalescent phases of leptospirosis ( n = 52) by enzyme-linked immunosorbent assays. (asm.org)
  • Monoclonal antibody (MAb)-based capture enzyme-linked immunosorbent assays (ELISAs) for the detection of anti-arboviral immunoglobulin G (IgG ELISAs) were developed for a comprehensive array of medically important arboviruses from the Alphavirus , Flavivirus , and Bunyavirus genera. (asm.org)
  • CA IX and hypoxia-inducible factor-1 α-subunit (HIF-1α) protein were quantified in 15 matched-paired WTs and NKs using enzyme-linked immunosorbent assays. (biomedcentral.com)
  • Diagnosis of bovine virus diarrhoea by two enzyme-linked immunosorbent assays. (google.ca)
  • The Use of Enzyme-Linked Immunosorbent Assays to Quantitate Proteins, Dori Germolec. (indigo.ca)
  • The sensitivity of the enzyme-linked amplified sorbent test (ELAST) was compared with those of other classic enzyme-linked immunosorbent assays (ELISAs), with or without previous acidic immunocomplex dissociation (ICD), in a series of samples at different stages of human immunodeficiency virus type 1 (HIV-1) infection. (nih.gov)
  • The confidence of proteome data was validated by enzyme-linked immunosorbent assays. (mdpi.com)
  • Possibly the best chapters in the book involve solid-phase extraction and enzyme-linked immunosorbent assays. (ift.org)
  • Comparison of a multiplexed fluorescent covalent microsphere immunoassay and an enzyme-linked immunosorbent assay for measurement of human immunoglobulin G antibodies to anthrax toxins. (cdc.gov)
  • An enzyme-linked immunosorbent assay for detection of IgG, IgA&IgM antibodies to rinderpest-virus in experimentally infected cattle. (springer.com)
  • The present invention relates to antigen-capture substrates useful for orienting capture antibodies for immunosorbent antigen determination. (freepatentsonline.com)
  • A technique using antibodies for detecting specific proteins. (usda.gov)
  • extremely sensitive and specific analytical technique using antibodies linked to an enzyme system to amplify sensitivity. (encyclopedia.com)
  • H. pylori IgG antibodies were measured through an enzyme-linked immunosorbent assay. (unboundmedicine.com)
  • It is also called as immunoblotting technique wherein specific antibodies are used to detect antigens. (openpr.com)
  • Detection of Candida antigen in sera of patients with candidiasis by an enzyme-linked immunosorbent assay-inhibition technique. (asm.org)
  • A total of 37 serum samples from 27 cancer patients were tested by an enzyme-linked immunosorbent assay-inhibition technique for the detection of Candida antigen. (asm.org)
  • The specific detection of foot and mouth disease virus whole particle antigen (140 S) by enzyme labelled immunosorbent assay. (springer.com)
  • Antigen requirement and specificity of enzyme-linked immunosorbent assay for detection of canine IgG against canine distemper viral antigen. (springer.com)
  • Detection of Mycoplasma bovis specific IgG in bovine serum by enzyme-linked immunosorbent assay. (springer.com)
  • The technique essentially requires any ligating reagent that can be immobilized on the solid phase along with a detection reagent that will bind specifically and use an enzyme to generate a signal that can be properly quantified. (wikipedia.org)
  • Development and evaluation of an enzyme-linked immunosorbent assay for use in the detection of bovine tuberculosis in cattle. (nih.gov)
  • Enzyme-linked immunosorbent assay for detection of human immunoglobulin G to lipopolysaccharide of spotted fever group rickettsiae. (naver.com)
  • The purpose of this review is to give an overview of recent advances in proteomic techniques for the detection and quantification of cytokines in melanoma research. (mdpi.com)
  • Various next generation molecular assay developed through advancements in the in-vitro detection techniques including genomics and proteomics are a growing trend in the global next generation molecular assay market and are expected to drive the growth of the next generation molecular assay market. (prsync.com)
  • Western blotting is a technique used for detection of protein. (openpr.com)
  • Improvements in the Passive Hemagglutination Technique for Serological Detection of Plant Viruses. (apsnet.org)
  • The single-step procedure is based on a column-switching technique which uses an immobilized antibody in an extraction column following concentration on a precolumn and separation on an analytical column. (biomedsearch.com)
  • An excellent and well arranged compilation of a range of antibody techniques. (booktopia.com.au)
  • An overview of application methods and techniques for use with your antibody. (abcam.com)
  • Development of a monoclonal antibody-based enzyme-linked immunosorbent assay for the analysis of 6-benzylaminopurine and its ribose adduct in bean sprouts. (cdc.gov)
  • An indirect competitive enzyme-linked immunosorbent assay (icELISA) was developed with a specific monoclonal antibody (mAb 3E5). (cdc.gov)
  • Use of enzyme-linked immunosorbent assay techniques with cross-reacting human sera in diagnosis of murine typhus and spotted fever. (naver.com)
  • Patients undergo a blood draw following diagnosis of breast cancer to assess levels of circulating tumor markers, calgranulin A and calgranulin B. Serum samples are evaluated by enzyme-linked immunosorbent assay for tumor marker expression. (clinicaltrials.gov)
  • New Generation Techniques in Rabies Diagnosis. (euttaranchal.com)
  • Enzyme labelled immunosorbent assay techniques in foot and mouth disease virus research. (springer.com)
  • In: "Immunoenzymatic Assay Techniques", R. Malvano ed. (springer.com)
  • Although this review is focused mainly on cancer and particularly melanoma, the discussed techniques are in general applicable to broad research field of biology and medicine, including stem cells, development, aging, immunology and intercellular communication. (mdpi.com)
  • These techniques provide a simple and convenient way to serodiagnose Mediterranean spotted fever and murine typhus with a single serum dilution. (naver.com)
  • Serum samples from 446 randomly selected persons belonging to different age groups and locations in Nigeria were tested for the presence of WSLV IgM using the flavivirus haemagglutination-inhibition (HI) test adopted to the solid-phase immunosorbent technique (SPIT). (meta.org)
  • Serum samples are evaluated by enzyme-linked immunosorbent assay for tumor marker expression. (clinicaltrials.gov)
  • For clinical evaluation of the four serological techniques, 260 paired acute and convalescent phase serum samples of suspected chikungunya cases were used. (scielosp.org)
  • HBP was measured using enzyme-linked immunosorbent technique in both serum & CSF. (scielo.br)
  • Another technique for measuring mRNA is reverse transcription followed by quantitative polymerase chain reaction (RT-qPCR). (news-medical.net)
  • All of these factors mandate that laboratories which provide diagnostic support for arboviruses have rapid and flexible serological techniques available to them. (asm.org)
  • However, in these studies laborious and poorly standardized cell-based serological techniques were used. (asm.org)
  • Immunoprecipitation is a versatile technique that enables isolation and purification of individual and complexed proteins. (abcam.com)
  • Some of the significant technologies incorporated in IVD include polymerase chain reaction (PCR), microarray techniques, sequencing technology, and mass spectrometry, which are used for test sample preparation. (pharmiweb.com)
  • There are a variety of techniques for sample preparation and visualization, and the method used should be tailored to the type of specimen under investigation and the degree of sensitivity required. (abcam.com)
  • Considering the high sensitivity, low cost, simplicity of equipment (only a plate reader), and possibility for full automation, ELAST appears to be a promising new tool for measuring viral load, especially in areas with few resources, in which the procedures based on molecular biology techniques may be difficult to install. (nih.gov)
  • Serologic tests offer opportunities for identification of M. bovis-infected animals not afforded by current diagnostic techniques. (nih.gov)
  • We report a newly developed fluorescence covalent microbead immunosorbent assay (FCMIA) for B. anthracis PA which was Luminex xMap technology. (cdc.gov)
  • Compared with the immunoassays, HPLC and LC-MS/MS offer more precise measures of the parent compound while minimizing measurement of metabolites, but they can also be more expensive, time-consuming, labor-intensive techniques and less standardized making them provider dependent. (ahrq.gov)
  • Measurement of Intraneuronal Free Calcium Using Fluorescent Probe Technique, Prasada Rao S. Kodavanti. (indigo.ca)
  • PCAs were detected by an indirect immunofluorescence technique using cryostat sections of rat gastric mucosa. (unboundmedicine.com)
  • Survey of Commercial Carnation Cultivars for Four Viruses in California by Indirect Enzyme-Linked Immunosorbent Assay. (apsnet.org)
  • During the last decade enzyme-linked immunosorbent assay has been a technique of major interest to those engaged in immunodiagnostics of human and animal diseases. (springer.com)
  • On the basis of techniques, the immunodiagnostics segment captured the highest market share in 2016, owing to the increase in demand for personalized medicines. (alliedmarketresearch.com)
  • A test of a new technique or variety, including biotech-derived varieties, done outside the laboratory but with specific requirements on location, plot size, methodology, etc. (usda.gov)
  • In this laboratory based course, students will learn to use current techniques in molecular and genetic engineering to address authentic research questions. (wpi.edu)
  • Do you want to incorporate modern techniques into your existing laboratory operations? (ift.org)
  • In vitro diagnostic (IVD) is a technique in which medical devices and reagents are used to examine specimens such as blood, urine, stool, tissues, and other body fluids, which are derived from the human body to detect diseases, conditions, and infections. (pharmiweb.com)
  • pronounced like the name "Eliza") is a testing technique used to detect and measure substances in biological samples. (neogen.com)
  • We observed the protein expression profiles in the epididymal luminal fluids in infertile and normal rats using isobaric tags for relative and absolute quantitation (iTRAQ) technique. (mdpi.com)
  • The content is divided into six main parts, the first of which presents techniques used in the biological characterization and transmission of viruses, while Part II covers purification and techniques concerning the physico-chemical properties of viruses. (springer.com)
  • However, the gene expression level can also be inferred by measuring the level of mRNA, which is achieved using a technique called Northern blotting. (news-medical.net)
  • immunoblot analysis and enzyme-linked immunosorbent analysis. (bio.net)
  • Simple high-performance liquid chromatographic column-switching technique for the on-line immunoaffinity extraction and analysis of flunitrazepam and its main metabolites in urine. (biomedsearch.com)
  • Rise in incidence & prevalence of chronic disease, technological advancements in IVD technique, and rise in healthcare expenditure fuel the market growth during the analysis period. (alliedmarketresearch.com)
  • Enzyme-Linked Immunosorbent Assay for Plant Viruses in Intact Leaf Tissue Disks. (apsnet.org)
  • Manipulation of an organism's genes by introducing, eliminating or rearranging specific genes using the methods of modern molecular biology, particularly those techniques referred to as recombinant DNA techniques. (usda.gov)
  • Included are basic RT-PCR techniques, cell culture systems, second-messenger signaling methods, and patch-clamp techniques. (indigo.ca)
  • of plastics material having a pore size of 10 microns or greater, through which the applied liquid sample must pass en route to the porous carrier material, the labelled specific binding reagent being freely soluble or dispersible in any liquid sample which enters the macroporous body. (google.com.au)
  • An outstanding collection of detailed, state-of-the-art techniques for studying many aspects of nervous system cell biology. (indigo.ca)
  • Tentatively, the global Next Generation Molecular Assay Market can be segmented on the basis of test type, technique, end user, and geography. (prsync.com)
  • The global hepatitis B diagnostic tests market is classified on the basis of test type, technique end use and geography. (medgadget.com)
  • Like the first volume, this text divides the chapters into four broad areas: Techniques for the Assessment of Toxicity in Whole Organisms, Cellular and Subcellular Toxicity, Contaminant Identification, and Impacts in Aquatic Ecosystems, and ends with a General Techniques section that anyone working in the field should find useful. (routledge.com)
  • Today, we're in the life sciences laboratories at Lancaster University to see some of the techniques we can use to determine if somebody is suffering from or has recently suffered from a virus. (futurelearn.com)
  • These powerful techniques will illuminate nervous system function, injury, degeneration, and the repair/regenerative process. (indigo.ca)
  • The first technique we're going to look at is western blotting, which was developed by W N Burnette in Seattle in the 1980s, based on earlier work by Ed Southern. (futurelearn.com)
  • And so western, which is the other technique we're looking at today, would actually be negative. (futurelearn.com)