A form of non-Hodgkin lymphoma having a usually diffuse pattern with both small and medium lymphocytes and small cleaved cells. It accounts for about 5% of adult non-Hodgkin lymphomas in the United States and Europe. The majority of mantle-cell lymphomas are associated with a t(11;14) translocation resulting in overexpression of the CYCLIN D1 gene (GENES, BCL-1).
A general term for various neoplastic diseases of the lymphoid tissue.
A group of heterogeneous lymphoid tumors generally expressing one or more B-cell antigens or representing malignant transformations of B-lymphocytes.
Any of a group of malignant tumors of lymphoid tissue that differ from HODGKIN DISEASE, being more heterogeneous with respect to malignant cell lineage, clinical course, prognosis, and therapy. The only common feature among these tumors is the absence of giant REED-STERNBERG CELLS, a characteristic of Hodgkin's disease.
The use of two or more chemicals simultaneously or sequentially in the drug therapy of neoplasms. The drugs need not be in the same dosage form.
Malignant lymphoma composed of large B lymphoid cells whose nuclear size can exceed normal macrophage nuclei, or more than twice the size of a normal lymphocyte. The pattern is predominantly diffuse. Most of these lymphomas represent the malignant counterpart of B-lymphocytes at midstage in the process of differentiation.
A group of heterogeneous lymphoid tumors representing malignant transformations of T-lymphocytes.
Malignant lymphoma in which the lymphomatous cells are clustered into identifiable nodules within the LYMPH NODES. The nodules resemble to some extent the GERMINAL CENTER of lymph node follicles and most likely represent neoplastic proliferation of lymph node-derived follicular center B-LYMPHOCYTES.
Antibodies obtained from a single clone of cells grown in mice or rats.
Administration of vaccines to stimulate the host's immune response. This includes any preparation intended for active immunological prophylaxis.
Unique genetically-controlled determinants present on ANTIBODIES whose specificity is limited to a single group of proteins (e.g., another antibody molecule or an individual myeloma protein). The idiotype appears to represent the antigenicity of the antigen-binding site of the antibody and to be genetically codetermined with it. The idiotypic determinants have been precisely located to the IMMUNOGLOBULIN VARIABLE REGION of both immunoglobin polypeptide chains.
A loose confederation of computer communication networks around the world. The networks that make up the Internet are connected through several backbone networks. The Internet grew out of the US Government ARPAnet project and was designed to facilitate information exchange.
A cyclic lipopeptide antibiotic that inhibits GRAM-POSITIVE BACTERIA.
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.
The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.
Partial cDNA (DNA, COMPLEMENTARY) sequences that are unique to the cDNAs from which they were derived.
Sequential operating programs and data which instruct the functioning of a digital computer.
Substances that are recognized by the immune system and induce an immune reaction.
Exclusive legal rights or privileges applied to inventions, plants, etc.
Endogenous tissue constituents that have the ability to interact with AUTOANTIBODIES and cause an immune response.
Substances elaborated by bacteria that have antigenic activity.
Proteins, glycoprotein, or lipoprotein moieties on surfaces of tumor cells that are usually identified by monoclonal antibodies. Many of these are of either embryonic or viral origin.
Antigens on surfaces of cells, including infectious or foreign cells or viruses. They are usually protein-containing groups on cell membranes or walls and may be isolated.
Substances elaborated by viruses that have antigenic activity.
Critical and exhaustive investigation or experimentation, having for its aim the discovery of new facts and their correct interpretation, the revision of accepted conclusions, theories, or laws in the light of newly discovered facts, or the practical application of such new or revised conclusions, theories, or laws. (Webster, 3d ed)
A superfamily of PROTEIN-SERINE-THREONINE KINASES that are activated by diverse stimuli via protein kinase cascades. They are the final components of the cascades, activated by phosphorylation by MITOGEN-ACTIVATED PROTEIN KINASE KINASES, which in turn are activated by mitogen-activated protein kinase kinase kinases (MAP KINASE KINASE KINASES).
Those individuals engaged in research.
A mitogen-activated protein kinase subfamily that regulates a variety of cellular processes including CELL GROWTH PROCESSES; CELL DIFFERENTIATION; APOPTOSIS; and cellular responses to INFLAMMATION. The P38 MAP kinases are regulated by CYTOKINE RECEPTORS and can be activated in response to bacterial pathogens.
Financial support of research activities.
Research that involves the application of the natural sciences, especially biology and physiology, to medicine.
Constituent composed of protein and phospholipid that is widely distributed in many tissues. It serves as a cofactor with factor VIIa to activate factor X in the extrinsic pathway of blood coagulation.
Calcium and magnesium salts used therapeutically in hepatobiliary dysfunction.
A class of lipoproteins of small size (18-25 nm) and light (1.019-1.063 g/ml) particles with a core composed mainly of CHOLESTEROL ESTERS and smaller amounts of TRIGLYCERIDES. The surface monolayer consists mostly of PHOSPHOLIPIDS, a single copy of APOLIPOPROTEIN B-100, and free cholesterol molecules. The main LDL function is to transport cholesterol and cholesterol esters to extrahepatic tissues.
Antibodies that react with self-antigens (AUTOANTIGENS) of the organism that produced them.
Immunoglobulin molecules having a specific amino acid sequence by virtue of which they interact only with the ANTIGEN (or a very similar shape) that induced their synthesis in cells of the lymphoid series (especially PLASMA CELLS).
The property of antibodies which enables them to react with some ANTIGENIC DETERMINANTS and not with others. Specificity is dependent on chemical composition, physical forces, and molecular structure at the binding site.
A class of immunoglobulin bearing mu chains (IMMUNOGLOBULIN MU-CHAINS). IgM can fix COMPLEMENT. The name comes from its high molecular weight and originally being called a macroglobulin.
Immunoglobulins produced in response to VIRAL ANTIGENS.
A cell line derived from cultured tumor cells.
CXCR receptors with specificity for CXCL12 CHEMOKINE. The receptors may play a role in HEMATOPOIESIS regulation and can also function as coreceptors for the HUMAN IMMUNODEFICIENCY VIRUS.
CXCR receptors that are expressed on the surface of a number of cell types, including T-LYMPHOCYTES; NK CELLS; DENDRITIC CELLS; and a subset of B-LYMPHOCYTES. The receptors are activated by CHEMOKINE CXCL9; CHEMOKINE CXCL10; and CHEMOKINE CXCL11.
Established cell cultures that have the potential to propagate indefinitely.
Benign and malignant neoplastic processes that arise from or secondarily involve the brain, spinal cord, or meninges.
IMMUNOGLOBULINS on the surface of B-LYMPHOCYTES. Their MESSENGER RNA contains an EXON with a membrane spanning sequence, producing immunoglobulins in the form of type I transmembrane proteins as opposed to secreted immunoglobulins (ANTIBODIES) which do not contain the membrane spanning segment.
Antibodies, often monoclonal, in which the two antigen-binding sites are specific for separate ANTIGENIC DETERMINANTS. They are artificial antibodies produced by chemical crosslinking, fusion of HYBRIDOMA cells, or by molecular genetic techniques. They function as the main mediators of targeted cellular cytotoxicity and have been shown to be efficient in the targeting of drugs, toxins, radiolabeled haptens, and effector cells to diseased tissue, primarily tumors.
B-cell lymphoid tumors that occur in association with AIDS. Patients often present with an advanced stage of disease and highly malignant subtypes including BURKITT LYMPHOMA; IMMUNOBLASTIC LARGE-CELL LYMPHOMA; PRIMARY EFFUSION LYMPHOMA; and DIFFUSE, LARGE B-CELL, LYMPHOMA. The tumors are often disseminated in unusual extranodal sites and chromosomal abnormalities are frequently present. It is likely that polyclonal B-cell lymphoproliferation in AIDS is a complex result of EBV infection, HIV antigenic stimulation, and T-cell-dependent HIV activation.
A medical specialty concerned with the hypersensitivity of the individual to foreign substances and protection from the resultant infection or disorder.
Studies beyond the bachelor's degree at an institution having graduate programs for the purpose of preparing for entrance into a specific field, and obtaining a higher degree.
Programs of training in medicine and medical specialties offered by hospitals for graduates of medicine to meet the requirements established by accrediting authorities.
Educational programs for dental graduates entering a specialty. They include formal specialty training as well as academic work in the clinical and basic dental sciences, and may lead to board certification or an advanced dental degree.
Educational programs for pharmacists who have a bachelor's degree or a Doctor of Pharmacy degree entering a specific field of pharmacy. They may lead to an advanced degree.
Dissertations embodying results of original research and especially substantiating a specific view, e.g., substantial papers written by candidates for an academic degree under the individual direction of a professor or papers written by undergraduates desirous of achieving honors or distinction.

Idiotype vaccination using dendritic cells after autologous peripheral blood stem cell transplantation for multiple myeloma--a feasibility study. (1/883)

The idiotype (Id) determinant on the multiple myeloma (MM) protein can be regarded as a tumor-specific marker. Immunotherapy directed at the MM Id may stem the progression of this disease. We report here on the first 12 MM patients treated at our institution with high-dose therapy and peripheral blood stem cell transplantation (PBSCT) followed by Id immunizations. MM patients received PBSCT to eradicate the majority of the disease. PBSCT produced a complete response in 2 patients, a partial response in 9 patients and stable disease in 1 patient. Three to 7 months after high-dose therapy, patients received a series of monthly immunizations that consisted of two intravenous infusions of Id-pulsed autologous dendritic cells (DC) followed by five subcutaneous boosts of Id/keyhole limpet hemocyanin (KLH) administered with adjuvant. Between 1 and 11 x 10(6) DC were obtained by leukapheresis in all patients even after PBSCT. The administration of Id-pulsed DC and Id/KLH vaccines were well tolerated with patients experiencing only minor and transient side effects. Two of 12 patients developed an Id-specific, cellular proliferative immune response and one of three patients studied developed a transient but Id-specific cytotoxic T-cell (CTL) response. Eleven of the 12 patients generated strong KLH-specific cellular proliferative immune responses showing the patients' immunocompetence at the time of vaccination. The two patients who developed a cellular Id-specific immune response remain in complete remission. Of the 12 treated patients, 9 are currently alive after autologous transplantation with a minimum follow-up of 16 months, 2 patients died because of recurrent MM and 1 patient succumbed to acute leukemia. These studies show that patients make strong anti-KLH responses despite recent high-dose therapy and that DC-based Id vaccination is feasible after PBSCT and can induce Id-specific T-cell responses. Further vaccine development is necessary to increase the proportion of patients that make Id-specific immune responses. The clinical benefits of Id vaccination in MM remain to be determined.  (+info)

DNA vaccination against the idiotype of a murine B cell lymphoma: mechanism of tumor protection. (2/883)

Several studies have shown that immunization with DNA, which encodes the idiotypic determinants of a B cell lymphoma, generates tumor-specific immunity. Although induction of antiidiotypic Abs has correlated with tumor protection, the effector mechanisms that contribute to tumor protection have not been clearly identified. This study evaluated the tumor protective effects of humoral and cellular immune mechanisms recruited by idiotype-directed DNA vaccines in the 38C13 murine B cell lymphoma model. Antiidiotypic Abs induced by DNA vaccination supported in vitro complement-mediated cytotoxicity of tumor cells, and simultaneous transfer of tumor cells and hyperimmune sera protected naive animals against tumor growth. However, in vitro stimulation of immune splenocytes with tumor cells failed to induce idiotype-specific cytotoxicity, and following vaccination, depletion of CD4 or CD8 T cell subsets did not compromise protection. Furthermore, protection of naive recipients against tumor challenge could not be demonstrated either by a Winn assay approach or by adoptive transfer of spleen and lymph node cells. Thus, in this experimental model, current evidence suggests that the tumor-protective effects of DNA vaccination can be largely attributed to idiotype-specific humoral immunity.  (+info)

Regulation of NOD mouse autoimmune diabetes by T cells that recognize a TCR CDR3 peptide. (3/883)

NOD mice spontaneously develop type I diabetes resulting from autoimmune destruction of their insulin-producing beta cells. Among the self-antigens targeted by NOD autoimmune T cells is a peptide, p277, from the sequence of the 60 kDa heat shock protein (hsp60). Common to the anti-p277 T cell populations of NOD mice is an idiotope, C9, that spans the CDR3 region of the C9 TCR. We now report: (i) that the C9 idiotope peptide can be presented directly to anti-C9 anti-idiotypic T cells by C9 T cells, (ii) that spontaneous anti-C9 anti-idiotypic T cell activity falls as disease progresses, but immunization can activate the anti-idiotypic T cells to regulate the autoimmune process, (iii) that the anti-idiotypic T cells secrete IFN-gamma, but appear to control the disease by down-regulating the IFN-gamma produced by the pathogenic population of anti-p277 T cells, (iv) that intrathymic administration of the C9 idiotope peptide at 1 week of age can accelerate the disease, and (v) that administering the p277 target peptide can up-regulate the anti-idiotypic T cells and arrest the disease process. Thus, the development of NOD diabetes can be regulated by a balance between anti-idiotypic and anti-target peptide autoimmunity, and anti-idiotypic regulation can lead to changes in the cytokine secretion of the autoimmune T cells involved in the disease process.  (+info)

Cutting edge: proteasome involvement in the degradation of unassembled Ig light chains. (4/883)

Several studies on disposal of nonsecreted Ig L chains have identified the endoplasmic reticulum as the site of degradation. Here, we examine degradation of a nonsecreted Ig L chain, T15L, and an experimentally endoplasmic reticulum-retained secretion-competent L chain, D16L, in the absence of H chains. We demonstrate that 1) degradation is specifically impaired by the proteasome-specific inhibitors carboxybenzyl-leucyl-leucyl-leucine vinyl sulfone (Z-L3VS) and lactacystin, 2) L chain degradation occurs early in the biosynthetic pathway, and 3) degradation does not require vesicular transport. Our findings indicate that previous assertions of L chain disposal within the endoplasmic reticulum must be modified. To our knowledge, we provide the first direct evidence supporting a new paradigm for removal of nonsecreted Ig L chains via dislocation to cytosolic proteasomes.  (+info)

Neonatal exposure to idiotype induces Schistosoma mansoni egg antigen-specific cellular and humoral immune responses. (5/883)

Exposure of neonatal mice to appropriate, cross-reactive Id (CRI) preparations alters immune responsiveness, ameliorates pathology, and prolongs survival of animals upon subsequent Schistosoma mansoni infection. However, because schistosome infections profoundly affect host immunobiology, which responses are effected by neonatal Id exposure alone and which responses are influenced by infection is unclear. To directly examine the schistosome soluble egg Ag (SEA)-specific immune responses altered by CRI exposure, neonatal mice were injected with CRI-expressing (CRI+) SEA-specific Ab preparations, SEA-specific Abs that did not express CRI (CRI-), or normal mouse Ig. At 9 wk of age, only mice that were neonatally exposed to CRI+ anti-SEA Abs displayed significant SEA-specific IgG serum levels and spleen cell proliferative responses. SEA-stimulated spleen cells from these CRI+-exposed mice also produced IFN-gamma, although not at significantly higher levels than mice receiving CRI- Id or normal mouse Ig. If CRI+-exposed mice were also injected with SEA at 8 wk of age, the 9-wk IFN-gamma responses were significantly higher than those of the other neonatal injection groups. The presence of both CRI and anti-CRI in the sera of animals neonatally injected with CRI, but receiving no exposure to S. mansoni Ags or infection, suggested a functional idiotypic network led to these responses. These data demonstrate that appropriate idiotypic exposure induces B and T cell responsiveness to the Ag recognized by the Id and support the hypothesis that neonatal idiotypic exposure can be an important immunoregulatory factor in schistosomiasis.  (+info)

Idiotype vaccination in human myeloma: generation of tumor-specific immune responses after high-dose chemotherapy. (6/883)

Igs contain unique portions, collectively termed idiotypes (Id), that can be recognized by the immune system. Id expressed by tumor cells in B-cell malignancies can be regarded as tumor-specific antigens and a target for vaccine immunotherapy. We have started a vaccination trial in multiple myeloma (MM) using Id-specific proteins conjugated to keyhole limpet hemocyanin (KLH) as immunogens and low doses of subcutaneous granulocyte-macrophage colony-stimulating factor (GM-CSF) or interleukin-2 (IL-2) as immunoadjuvants. Twelve patients who had previously been treated with high-dose chemotherapy followed by peripheral blood progenitor cell (PBPC) transplantation entered this study from August 1995 to January 1998. All patients were in first remission at the time of vaccination. They received subcutaneous injections of Id vaccines and immunoadjuvants in an outpatient setting. The generation of Id-specific T-cell proliferative responses was documented in 2 patients, whereas a positive Id-specific delayed-type hypersensitivity (DTH) reaction was observed in 8 of the 10 patients studied. DTH specificity was confirmed in 1 patient by investigating the reactivity to synthetic peptides derived from the VDJ sequence of the tumor-specific Ig heavy chain. None of the patients generated soluble immune responses to Id, whereas the generation of soluble and cellular immune responses to KLH was observed in 100% and 80%, respectively. Eleven patients completed the treatment, whereas 1 patient failed to finish owing to progression of disease. Freedom from disease progression (FFDP), measured from the date of first Id/KLH injection to the date of first treatment after vaccination or last follow-up, ranged from 9 to 36 months. These data indicate that the immune competence status of MM patients is still susceptible to specific immunization after high-dose chemotherapy and PBPC transplantation. It remains to be determined whether generation of Id-specific immune responses can reduce the relapse rate of patients with minimal residual disease.  (+info)

scFv multimers of the anti-neuraminidase antibody NC10: length of the linker between VH and VL domains dictates precisely the transition between diabodies and triabodies. (7/883)

Single-chain Fv antibody fragments (scFvs) incorporate a polypeptide linker to tether the VH and VL domains together. An scFv molecule with a linker 5-12 residues long cannot fold into a functional Fv domain and instead associates with a second scFv molecule to form a bivalent dimer (diabody). Direct ligation of VH and VL domains further restricts association and forces three scFv molecules to associate into a trivalent trimer (triabody). We have defined the effect of linker length on scFv association by constructing a series of scFvs from anti-neuraminidase antibody NC10 in which the linker varied from one to four glycine residues. NC10 scFv molecules containing linkers of three and four residues showed a strong preference for dimer formation (diabodies), whereas a linker length of one or two glycine residues prevented the formation of diabodies and directed scFv association into trimers (triabodies). The data suggest a relatively strict transition from dimer (diabody) to trimer (triabody) upon reduction of the linker length from three to two glycine residues. Modelling studies are consistent with three residues as the minimum linker length compatible with diabody formation. Electron microscope images of complexes formed between the NC10 scFv multimers and an anti-idiotype Fab' showed that the dimer was bivalent for antigen binding and the trimer was trivalent.  (+info)

Molecular and idiotypic analyses of the antibody response to Cryptococcus neoformans glucuronoxylomannan-protein conjugate vaccine in autoimmune and nonautoimmune mice. (8/883)

The antibody response to Cryptococcus neoformans capsular glucuronoxylomannan (GXM) in BALB/c mice frequently expresses the 2H1 idiotype (Id) and is restricted in variable gene usage. This study examined the immunogenicity of GXM-protein conjugates, V (variable)-region usage, and 2H1 Id expression in seven mouse strains: BALB/c, C57BL/6, A/J, C3H, NZB, NZW, and (NZB x NZW)F(1) (NZB/W). All mouse strains responded to vaccination with GXM conjugated to tetanus toxoid (TT), the relative magnitude of the antibody response being BALB/c approximately C3H > C57BL/6 approximately NZB approximately NZW approximately NZB/W > A/J. Analysis of serum antibody responses to GXM with polyclonal and monoclonal antibodies to the 2H1 Id revealed significant inter- and intrastrain differences in idiotype expression. Thirteen monoclonal antibodies (MAbs) (two immunoglobulin M [IgM], three IgG3, one IgG1, three IgG2a, two IgG2b, and two IgA) to GXM were generated from one NZB/W mouse and one C3H/He mouse. The MAbs from the NZB/W mouse were all 2H1 Id positive (Id(+)) and structurally similar to those previously generated in BALB/c mice, including the usage of a V(H) from the 7183 family and Vkappa5.1. Administration of both 2H1 Id(+) and Id(-) MAbs from NZB/W and C3H/H3 mice prolonged survival in a mouse model of cryptococcosis. Our results demonstrate (i) that V-region restriction as indicated by the 2H1 Id is a feature of both primary and secondary responses of several mouse strains; and (ii) that there is conservation of V-region usage and length of the third complementarity-determining region in antibodies from three mouse strains. The results suggest that V-region restriction is a result of antibody structural requirements necessary for binding an immunodominant antigen in GXM.  (+info)

Caulfield, M J.; Proffitt, M R.; and Cerny, J, Induction of idiotype-specific suppressor cells (sc) with antigen/antibody complexes. Abstr. (1982). Subject Strain Bibliography 1982. 1718 ...
Two synergizing antigen-specific helper T (Th) cell populations are required for an optimal TEPC15 (T15)-dominated antiphosphorylcholine (PC) plaque- forming cell response . In these studies, the two Th cell sets are shown to differ in their requirements for recognition of self-major histocompatibility complex (MHC)-encoded determinants by testing the ability of Th cells from F(1) {arrow} parent bone marrow chimeras to collaborate with PC-specific B cells bearing MHC-encoded determinants of either parental haplotypes. Previous studies have shown that one antigen-specific Th cell population is required for T-dependent anti-PC responses and activates PC-specific B cells only if the hapten, PC, is physically linked to the priming antigen. This Th cell, referred to as ThMHC, induces anti-PC responses that are mainly non-T15 in character, and it appears to be identical to the conventional antigen- specific Th cell. In these experiments, using T cells from (A X B)F(1) {arrow} parent A chimeras, ThMHC ...
Ossendorf V, Cornely O, Draube A, Monsef I, Engert A, Skoetz N. Idiotype vaccination for Non-Hodgkin lymphoma. Cochrane Database of Systematic Reviews 2016, Issue 2. Art. No.: CD008964. DOI: 10.1002/14651858.CD008964. ...
Rubin, B; Hertel, wulff B.; and Kimura, A, Alloantigen-specific idiotype-bearing receptors on mouse t lymphocytes. I. Specificity characterization and genetic association with the heavy-chain igg allotype. (1979). Subject Strain Bibliography 1979. 4576 ...
Antigen-binding receptors on T lymphocytes and IgG antibodies with the same antigen-binding specificity as the T-cell receptors display shared or identical idiotypes. This was shown using a system where adult F1 hybrid rats between two inbred strains were inoculated with T lymphocytes from one parental strain. Such F1 hybrid rats produce antibodies directed against idiotypic determinants present on IgG alloantibodies, produced in the T donor genotype strain and with specificity for the alloantigens of the other parental strain. The idiotypic nature of the F1 antialloantibody serum against the parental alloantibodies was demonstrated both by indirect hemagglutination tests or by gel diffusion using alloantisera with different specificity as targets. Furthermore, the F1 anti-T-lymphocyte sera could be shown to contain antibodies against idiotypic parental T lymphocytes as well. This was shown by the capacity of the antisera, in the presence of complement, to wipe out the relevant parental T-cell ...
Dive into the research topics of A common idiotype expressed on a murine anti-Sm monoclonal antibody and antibodies in SLE sera. Together they form a unique fingerprint. ...
Recently the minor B cell subpopulation that expresses the CD5 (Leu-1) antigen has been implicated as a source of IgM autoantibodies. Chronic lymphocytic leukemia (CLL), the most common leukemia in humans, represents a malignancy of small B lymphocytes that also express the CD5 antigen. However, little is known concerning the antibody variable region genes (V genes) that are used by these malignant CD5 B cells. We have found that a relatively high frequency of CLL patients have leukemic B cells with surface immunoglobulin (sIg) recognized by 17.109, a murine mAb specific for a kappa light chain associated crossreactive idiotype (CRI) associated with rheumatoid factor and other IgM autoantibodies. Flow cytometric analyses revealed that the relative expression of the 17.109-CRI by circulating leukemic B cells was directly proportional to the levels of sIg kappa light chain, indicating that there exists stable idiotype expression in the leukemic population. To examine this at the molecular level, ...
Looking for Idiotypes? Find out information about Idiotypes. The unique amino acid sequence and corresponding three-dimensional structure of the variable region of an immunoglobulin molecule that determines its... Explanation of Idiotypes
Rabbit anti-idiotypic antibodies were prepared by injection of specifically purified anti-p-azobenzoate antibodies (D) from individual donor rabbits. Benzoate derivatives were found to be strong inhibitors of the reactions of D with anti-D antisera. There was a close correlation between the combining affinities of the benzoate derivatives used and their effectiveness as inhibitors. Compounds tested that are chemically unrelated to benzoate were ineffective. The results indicate either that the combining site of anti-benzoate antibody is part of an important idiotypic determinant, which is sterically blocked by hapten, or that the hapten induces a conformational change which alters idiotypic determinants not involving the active site. Such conformational changes, if they occur, must be restricted since hapten has little effect on the reactions of F(ab)2 fragments of anti-benzoate antibodies with antisera directed to rabbit fragment Fab and no detectable effect on reactions with antibodies ...
Author(s): Markus Brede ,Ulrich Behn Subject(s): CX.3 Category: Abstract:. The talk deals with modelling a subsystem of the immune system, the so-called idiotypic network. Idiotypic networks, a concept conceived by N.K. Jerne in 1974, are functional networks of interacting antibodies and B-cells. In principle, Jernes framework provides solutions to many issues in immunology, such as immunological memory, mechanisms for antigen recognition and the question of self/non-self discrimination. Explaining the interconnection between the elementary components local dynamics, network formation and architecture, and possible modes of global system function appears to be an ideal playground of statistical mechanics. We present a simple cellular automaton model based on a graph representation of the system. From a simplified description of idiotypic interactions rules for the random evolution of networks of occupied and empty sites on these graphs are derived. In certain biologically relevant parameter ...
The anti-idiotypic (anti-Id) antibody (Ab) 9G4 binds a cross-reactive idiotope (CRI) present in a select group of human autoantibodies. This Id has been localized to the portion of immunoglobulin (Ig) heavy (H) chains encoded by the VH4-21 gene segment, a member of the human VH4 family. This gene segment is utilized by essentially all cold agglutinin (CA) Abs with I/i specificity isolated from patients with CA disease stemming from chronic lymphoproliferative disorders. In this study, mutational analysis of a CA has been used to determine the structural basis for 9G4 binding to Abs utilizing the VH4-21 gene segment. Recombinant CA H chain mutants were produced and their 9G4 reactivity determined. Mutants were generated by exchanging VH4-21 sequences in the FR1, CDR1, and CDR2 with corresponding sequences from a closely related gene segment V71-2, a VH4 family member that is associated neither with Abs having CA activity nor with Abs that react with 9G4. The results indicate that the motif AVY at ...
Purchase Idiotypes in Medicine: Autoimmunity, Infection and Cancer - 1st Edition. Print Book & E-Book. ISBN 9780444828071, 9780080534435
A monoclonal anti-idiotypic antibody specific to a human IgG 1 type monoclonal antibody possessing specificity to nicotinic acetylcholine receptor; a method for the production of the aforementioned monoclonal anti-idiotypic antibody by the steps of immunizing an animal with a human IgG 1 type monoclonal antibody specific to nicotinic acetylcholine receptor, collecting antibody-producing cells from the animal, fusing the collected cells with neoplastic cells, selecting from the product of fusion a hybridoma capable of producing a monoclonal anti-idiotypic antibody specific to the human IgG 1 type monoclonal antibody possessing specificity to nicotinic acetylcholine receptor, propagating the selected hybridoma thereby giving rise to said monoclonal anti-idiotypic antibody, and collecting the produced monoclonal anti-idiotypic antibody; and use of the monoclonal anti-idiotypic antibody as a reagent and as an adsorbent.
A monoclonal anti-idiotypic antibody specific to a human IgG1 type monoclonal antibody possessing specificity to nicotinic acetylcholine receptor; a method for the production of the aforementioned monoclonal anti-idiotypic antibody by the steps of immunizing an animal with a human IgG1 type monoclonal antibody specific to nicotinic acetylcholine receptor, collecting antibody-producing cells from the animal, fusing the collected cells with neoplastic cells, selecting from the product of fusion a hybridoma capable of producing a monoclonal anti-idiotypic antibody specific to the human IgG1 type monoclonal antibody possessing specificity to nicotinic acetylcholine receptor, propagating the selected hybridoma thereby giving rise to said monoclonal anti-idiotypic antibody, and collecting the produced monoclonal anti-idiotypic antibody; and use of the monoclonal anti-idiotypic antibody as a reagent and as an adsorbent.
Hybridoma-derived idiotype vaccines have been used for the experimental treatment of human lymphoma over the last twenty years, providing evidence of biological efficacy, clinical efficacy and clinical benefit. However, the product that has come closer to regulatory approval is unlikely to clear that hurdle due to the insufficiently robust data obtained in a recently closed clinical trial. This review aims at discussing the reasons for hybridoma-derived idiotype vaccines, more difficult to produce but also more successful than recombinant idiotype vaccines so far, are unlikely to gain regulatory approval. In particular, it is necessary to examine the many peculiar features of this therapeutic approach in a broader context, with special attention to concepts like customized active immunotherapy and randomization. Most published trials based on hybridoma-derived idiotype vaccines are being analyzed, together with the yet non-peer reviewed data from the only randomized study conducted so far with this
OBJECTIVE: To investigate whether autoreactive mechanisms occur in Lyme disease (LD) by determining IgA, IgG and IgM rheumatoid factor (RF) concentrations and RF associated cross reactive idiotype (CRI) expression in the serum of LD patients, with comparison to patients with rheumatoid arthritis (RA). METHODS: The RF isotype profiles were determined in 59 patients with LD; erythema migrans (EM) (n=19), neuroborreliosis (NB) (n=20) and Lyme arthritis (LA) (n=20). Mouse monoclonal antibodies (mAbs) G6 and G8 (V(H)1 gene associated), D12 (V(H)3 gene associated) and C7 (V(kappa)III gene associated) were then used to determine the RF associated CRI expression on IgM antibodies in 16 of these LD patients (eight seropositive for RF); (EM (n=3), NB (n=6), LA (n=7)). RESULTS: Seven (18%) patients with either NB or LA had increased concentrations of IgA RF compared with none with EM. Significant differences in the number of patients with raised concentrations of IgG RF or IgM RF were not found between the LD
High-dose therapy followed by autologous transplantation has improved survival in MM, however all patients eventually relapse, indicating the need for consolidation. Activation of idiotype-specific T cells by vaccination with autologous tumorprotein-loaded dendritic cells has been demonstrated. So-called Vaccibodies (consisting of two scFv (single chain Fragment variable) specific for surface molecules on APC (MHCII, CD40) linked to two exchangeable scFv from the mouse M315 myeloma protein and a Cγ3 dimerizing domain) elicit anti-Id antibodies in mice even in the absence of adjuvant, and stimulate Id-specific CD4+ T cells in vitro ...
Cell surface receptors for the 86-kDa glycoprotein (gp86) of human cytomegalovirus (HCMV) were identified by using two monoclonal anti-idiotype antibodies that bear the internal image of gp86. These antibodies bound to cells permissive for HCMV infection by both ELISA and immunofluorescence assay and inhibited HCMV plaque formation in human embryonic lung (HEL) cells. Immunoblot analysis showed specific binding of both internal image anti-idiotype antibodies as well as gp86 to an HEL cell membrane protein with an approximate molecular mass of 92.5 kDa. In addition, immunoprecipitation of radiolabeled membrane and cell surface proteins from human foreskin tissue, human foreskin fibroblasts, or HEL cells showed specific binding of anti-idiotype antibody predominantly to the 92.5-kDa protein.. ...
Many monoclonal antibodies that react with the lacto-N-fucopentaose III (LNF III) antigenic determinant, Gal beta 1-4(Fuc alpha 1-3)GlcNAc beta 1-3Gal beta 1-4Glc, have been described recently. The terminal trisaccharide of this determinant, fucosyllactosamine, is present on glycolipids and glycoproteins and on the surface of granulocytes, monocytes, and other cells. To study the structural and genetic diversity of these antibodies, syngeneic anti-idiotypic monoclonal antibodies were produced in BALB/c mice against PMN 6, a monoclonal antibody directed against this sequence. Anti-idiotypic antibodies 6B1 and 6C4 reacted with 50% of a panel of 20 anti-LNF III monoclonal antibodies, whereas 6A3 reacted strongly only with PMN 6. This indicates that the determinants recognized by 6C4 and 6B1 represent major cross-reactive idiotopes of this family of antibodies. The binding of idiotypic antibodies to a glycolipid bearing this antigenic determinant was completely inhibited by the three anti-idiotypic ...
Immunogenicity of Protein-315 and its Fab fragment (Fab-315) for isologous and heterologous strains of mice was investigated by comparing the characteristics of the anti-idiotypic response produced in BALB/c and A/J mice. The ability of these anti-idiotypic antisera to compete with DNP-lys for the ligand-binding site of Protein-315 were analyzed by comparing their hapten inhibition curves.. The anti-idiotypic responses of BALB/c mice to Protein-315 and to Fab-315 were similar to one another with regard to antibody specificity and sensitivity to inhibition by hapten, suggesting that both forms were equally immunogenic in inbred BALB/c mice. This observation indicates that the Fc portion of Protein-315 is not essential for the induction of anti-idiotypic response. Both BALB/c and A/J mice recognized the same antigenic determinants on Fab-315 since the anti-idiotypic antibodies produced by these animals were indistinguishable with regard to their interaction with Protein-315, Fv-315, and ...
B-cell lymphomas express surface immunoglobulin (immunoglobulin) containing unique idiotypic (idiotype) determinants which may be exploited as tumor specific markers. The inventor has produced murine monoclonal antibodies (MAbs) reactive with the idiotype marker derived from 67 patients with low grade, follicular, small cleaved cell lymphoma. Out of 199 monoclonal antibodies, 47 (24%) were found to react with pooled normal human serum immunoglobulin in concentrations ranging from 0.6 μg/ml to 160 μg/ml. Of these 40 monoclonal antibodies, 90% cross-reacted with idiotype present in normal serum in levels |50 μg/ml. Thirty-two of these anti-idiotypes were directed against a shared idiotope expressed on another patients lymphoma cells. The frequency of shared idiotope expression defined by each antibody ranged from 0.26% to 3.9% of the B-cell lymphomas tested. A panel of five anti-idiotype antibodies reacted with 80% of AIDS associated lymphomas. Based on the reactivity with these monoclonal antibodies,
Cardiac Myosin and Autoimmune Myocarditis (N. Rose et al.). Intracellular Autoantigens: Diagnostic Fingerprints but Aetiological Dilemmas (E. Tan et al.).. Significance of Carbohydrate Components of Cell Surfaces (T. Feizi).. How is Tolerance Generated (G. Nossal).. Therapeutic Immune Regulation in Experimental Interstitial Nephritis with Suppressor T-Cells and Their Soluble Factors (C. Kelly et al.).. Regulation of HLA Class II Expression and Its Role in Autoimmune Disease (M. Feldmann).. Idiotypes and Autoimmunity (J. Kearney et al.).. Molecular Basis for the Cross-reactive Idiotypes on Human Anti-IgG Autoantibodies (D. Carson et al.).. Autoimmunity and Immunodeficiency Disease (F. Rosen).. Deficiency of the Effector Mechanisms of the Immune Response and Autoimmunity (P. Lachmann & M. Walport).. Monoclonal Anti-Ia Antibody Therapy in Animal Models of Autoimmune Disease (H. McDevitt et al.).. Index of Contributors.. Subject Index.. ...
A panel of idiotypically cross-reactive murine monoclonal antibodies (Mabs) isolated in response to fluorescein (Fl) was generated and segregated based on Fl affinity. Intermediate affinity prototype Mab 9-40 (IgG1, $\kappa$; K$\sb{\rm a}$ = 3.3 $\times$ 10$\sp7$M$\sp{-1}$) possessed $>$90% active site quaternary structural homology to the intermediate affinity 9-40 idiotype family (comprised of 12-40, 3-24, 10-25, 5-14 and 5-27). The 9-40 family was 40-100% and 10-40% idiotypically related to high affinity prototype Mab 4-4-20 (IgG2a, $\kappa$; K$\sb{\rm a}$ = 1.8 $\times$ 10$\sp{10}$M$\sp{-1}$) and low affinity prototype Mabs 3-13 and 3-17 (K$\sb{\rm a}$ $\sim$ 5.0 $\times$ 10$\sp4$M$\sp{-1}$), respectively. Mabs 4-4-20 and 3-13/3-17 were idiotypically distinct. This anti-Fl panel spanned a greater affinity range than any previously reported idiotype family and was exploited to define specific active site residues (and their interactions with antigen) responsible for the observed Fl binding ...
TY - JOUR. T1 - The physiology of anti-idiotypic interactions. T2 - From clonal to paratopic selection. AU - Greally, John M.. PY - 1991. Y1 - 1991. N2 - On theoretical and experimental grounds, it has been proposed that the idiotypes of immunoglobulins and of T cell receptors are composed of multiple paratopes, as opposed to a single paratope and several idiotopes. This necessitates a revision of some of the basic principles of anti-idiotypic reactions. It is also possible to infer the presence of the same or similar paratopes on different idiotypes. A paratope cannot therefore be regarded as restricted to or unique on an idiotype. For these reasons, the perception of immunological specificity in terms of clonal units is misleading. This review proposes instead that the physiological unit of immunological specificity and regulation is the paratope. This essential alteration in the perception of the immune system is referred to as paratopic selection. The approach is assessed in terms of ...
Specific Interference with the Determination of the Tumour-Associated Glycoprotein 72 by Human Anti-Idiotypic Antibodies Formed after Treatment with the Anti-Tumour-Associated Glycoprotein 72 Antibody ...
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Three IgM monoclonal anti-DNA antibodies were produced by hybridoma techniques from an MRL-lpr/lpr mouse using denatured DNA (dDNA) as the selection antigen. All three antibodies also bound poly(dT), poly(rA), and the single-stranded random copolymer poly(dI,dT), and each antibody displayed a unique preference for a limited array of other ribo- and deoxyribopolynucleotides based on direct binding as well as inhibition studies. Inability to identify a common primary structure in the polynucleotides reactive with each antibody suggested that higher ordered structures may be important. This notion was supported by the finding that oligomers of thymidine of 25-30 nucleotides or less were ineffective in blocking antibody binding to dDNA or poly(dT). However, deliberate destabilization of putative secondary structures by decreasing counterion concentration and increasing temperature had little effect on antibody binding to poly(dT). Since the antigenic polynucleotides in general contain little known ...
An Idiotypic sequence of the light chain of the myeloma protein M315 produced by the MOPC315 tumor has been defined. This sequence is presented on MHC class II molecules to Id-specific CD4+ T cells. T cell receptor (TCR) genes from this clone were isolated and used to establish a TCR-transgenic mouse. Such TCR-transgenic mice are resistant to challenge with MOPC315 cells and the resistance can be transferred to other mice with purified Id-specific CD4+ T cells. Further experiments have demonstrated that myeloma protein secreted by the s.c. myeloma prime dendritic cells in the tumor with Id. These Id-primed dendritic cells then stimulate CD4 Id-specific CD4+ T cells that as activated cells somehow kill the myeloma cells.. In parallel, in vitro models have established mature B cells from pro-B cells and further generated membrane immunoglobulin B cells or plasma cells by additional stimuli , which may be improved by lymph node dendritic cells as well as antigen selected as idiotype specific ...
Anti-idiotype vaccination represents an innovative approach to target tumor-associated antigen-expressing cells. This approach comes directly from Jernes idiotypic network theory, which postulates that due to the huge potentiality for diversity of the immunoglobulin variable regions, the idiotype repertoire can mimic the universe of self and foreign epitopes [1].. NeuGc-containing gangliosides are attractive targets for cancer immunotherapy because these glycolipids are non-self antigens in humans [2, 3]. In contrast, they have been detected in different human tumors by antibodies and chemical analysis [4-6]. Recent experimental data suggest that N-glycolyl-GM3 ganglioside (NeuGcGM3) is relevant for tumor biology [7].. mAb-1E10 [8] is an IgG1 anti-idiotype (Ab2) mAb obtained by immunizing Balb/c mice with mAb-P3 (Ab1) [9] coupled to keyhole limpet hemocyanin (KLH) in the presence of Freunds adjuvant. This Ab2 inhibited the binding of mAb-P3 to NeuGcGM3 ganglioside. mAb-1E10 induced an ...
TY - JOUR. T1 - Emergence of immunoglobulin variants following treatment of a B cell leukemia with an immunotoxin composed of antiidiotypic antibody and saporin. AU - Glennie, M. J.. AU - McBride, H. M.. AU - Stirpe, F.. AU - Thorpe, P. E.. AU - Worth, A. T.. AU - Stevenson, G. T.. PY - 1987. Y1 - 1987. N2 - The potency and specificity of immunotoxins consisting of monoclonal antiidiotype conjugated to the ribosome-inactivating protein, saporin, have been evaluated in the treatment of guinea pig L2C B lymphocytic leukemia. The immunotoxins were therapeutically much more effective than their parent antibodies. Their specificity reflected that of their antiidiotype component. Although the leukemia emerged eventually in most animals treated with these conjugates, most of the cells showed altered Ig expression, which rendered them resistant to the therapy. Commonly, the emerging cells had lost μ heavy chain production, leaving them negative for intracellular, surface, and secreted IgM, but still ...
IDIOTYPES according to the free Medical Dictionary. The unique and characteristic parts of an antibodys variable region, which can themselves serve as antigens.
BACKGROUND: Therapeutic idiotypic (Id) vaccination is an experimental treatment for selected B cell malignancies. A broader use of Id-based vaccinatio
References for Abcams Anti-Progesterone Receptor (phospho S162) antibody [1064/E2] (ab58564). Please let us know if you have used this product in your…
E. S. Aleksandrova, F. Koralewski, M. I. Titov, A. V. Demin, A. N. Ignatova, A. V. Kozyr, A. V. Kolesnikov, A. Tramontano, S. Paul, D. Thomas, A. G. Gabibov, A. Friboulet ...
A major challenge in vaccinology is to predict vaccine efficacy [31-33]. Here, we used a multiparametric systems biology approach to identify gene signatures predictive of an immune response, using an experimental platform based on PBMCs from 6 HCV-positive subjects stimulated ex vivo with the IGKV3-20 light-chain protein, as candidate idiotype vaccine.. The cytokine pattern induced by IGKV3-20 was assessed by ELISA in culture supernatant of stimulated PBMCs, after 24 h or 6 days of incubation (Figure 1).. The results show that the stimulation induces an overall significant production of both Th1 (TNF-α) and Th2 cytokines (IL-6 and IL-10), with a prevalence of the latters. However, specific samples consistently show very different levels of TNF-α and IL-6 production, which are highest for samples BE and DN and lowest for sample MML. These results, although based on a small cohort, indicate a significant difference in the individual response to the same antigen and, in particular, suggest that ...
Polyclonal antibody for NR3C3/PGR detection. Host: Rabbit.Size: 100μg/vial. Tested applications: IHC-P. Reactive species: Human. NR3C3/PGR information: Molecular Weight: 98981 MW; Subcellular Localization: Nucleus. Cytoplasm. Nucleoplasmic shuttling is bo
Rabbit Polyclonal ID4 antibody N-Term for WB. Published in 2 Pubmed References. Order this anti-ID4 antibody. | Product number ABIN2780374
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Our laboratory is developing cancer vaccines that are being evaluated in animal models for their protective activity against tumors before they are administered to cancer patients. The vaccines are composed of antibodies mimicking tumor antigens (anti-idiotypic antibodies) or the antigens expressed in viruses (recombinant adeno- or vaccinia-viruses) and are selected based on their high probability of inducing both humoral and cellular immune responses in patients. The animal models we have developed for preclinical evaluation of the vaccines closely mimic the condition in cancer patients. In other studies which have reached clinical trials, we are evaluating the vaccinated patients immune responses to their tumors ...
This paper evaluates a representative sample of the best anti-ID and pro-ID publications and presents a conclusion as to the present state of the evidence and arguments regarding these positions. Published in Origins, n. 63.
Although there is multiple evidence for the functional heterogeneity and polyclonality of TRAb (1 - 3) both statements are questioned by some investigators (4, 5). Furthermore there is growing...
Η δημιουργία κι ο εμπλουτισμός της Πλατφόρμας τεκμηρίωσης των ερευνητικών αποτελεσμάτων και των ερευνητών (CRIS) Πανδώρα, έγινε στο πλαίσιο του Υποέργου 4 «Προμήθειες Εξοπλισμού Λογισμικού» της πράξης «Ψηφιακές υπηρεσίες ανοιχτής πρόσβασης της βιβλιοθήκης του Πανεπιστημίου Πειραιώς» με κωδικό ΟΠΣ «304169», του Επιχειρησιακού Προγράμματος Ψηφιακή Σύγκλιση ...
Η δημιουργία κι ο εμπλουτισμός της Πλατφόρμας τεκμηρίωσης των ερευνητικών αποτελεσμάτων και των ερευνητών (CRIS) Πανδώρα, έγινε στο πλαίσιο του Υποέργου 4 «Προμήθειες Εξοπλισμού Λογισμικού» της πράξης «Ψηφιακές υπηρεσίες ανοιχτής πρόσβασης της βιβλιοθήκης του Πανεπιστημίου Πειραιώς» με κωδικό ΟΠΣ «304169», του Επιχειρησιακού Προγράμματος Ψηφιακή Σύγκλιση ...
Ga-zi-an-tep-li sa-na-yi-ci ve tüc-car-lar, kriz söy-len-ti-le-ri-ne ve fe-la-ket tel-lal-la-rı-na inat ge-ce-gün-düz ça-lı-şıp üre-ti-yor, dün-ya-nın her ta-ra-fı-na ih-ra-cat ya-pı-yor, ül-ke-ye dö-viz ka-zan-dı-rı-yor...
Neuroblastoma treatment with chimeric anti-disialoganglioside GD2 antibody ch14.18 showed objective anti-tumor responses. Production of anti-idiotypic antibodies (Ab2) against ch14.18 (Ab1) in some cases was positively correlated with a more favorable prognosis. According to Jernes network theory, a subset of anti-idiotypic antibodies (Ab2beta) form an internal image of the antigen and induce antibodies (Ab3) against the original antigen. The molecular origin of the anti-idiotypic antibody response in tumor patients was not investigated previously. To clone anti-idiotypic antibodies, B-cells of a ch14.18-treated neuroblastoma patient with Ab2 serum reactivity were used to construct antibody phage display libraries (Methods described in Arthritis Rheum. 2000, 43:2722-2732). Upon repetitive selection of lambda and kappa Fab-phage display libraries on target antigens ch14.18 and the murine equivalent 14G2a, positive binders were enriched. Selected Ab2-clones GK2 and GK8 as well as another 38 ...
Eight monoclonal antibodies from the primary response of C57BL/6 mice against the hapten (4-hydroxy-3-nitrophenyl)acetyl (NP) were isolated. The antibodies carry lambda 1 light chains and have similar affinities for the immunizing hapten. Sequence analysis at the level of mRNA reveals that all antib …
General It is not known whether Simulect use will have a long-term effect on the ability of the immune system to respond to antigens first encountered during Simulect -induced immunosuppression. Immunogenicity Of renal transplantation patients treated with Simulect and tested for anti-idiotype antibodies, 4/339 developed an anti-idiotype antibody response, with no deleterious clinical effect upon the patient. In none of these cases was there evidence that the presence of anti-idiotype antibody accelerated Simulect clearance or decreased the period of receptor saturation. In Study 2, the incidence of human anti-murine antibody (HAMA) in renal transplantation patients treated with Simulect was 2/138 in patients not exposed to muromonab-CD3 and 4/34 in patients who subsequently received muromonab-CD3. The available clinical data on the use of muromonab-CD3 in patients previously treated with Simulect suggest that subsequent use of muromonab-CD3 or other murine anti-lymphocytic antibody preparations ...
Understanding the molecular mechanisms of immunological memory assumes importance in vaccine design. We had earlier hypothesized a mechanism for the maintenance of immunological memory through the operation of a network of idiotypic and anti-idiotypic antibodies (Ab2). Peptides derived from an internal image carrying anti-idiotypic antibody are hypothesized to facilitate the perpetuation of antigen specific T cell memory through similarity in peptide-MHC binding as that of the antigenic peptide. In the present work, the existence of such peptidomimics of the antigen in the Ab2 variable region and their similarity of MHC-I binding was examined by bioinformatics approaches. The analysis employing three known viral antigens and one tumor-associated antigen shows that peptidomimics from Ab2 variable regions have structurally similar MHC-I binding patterns as compared to antigenic peptides, indicating a structural basis for memory perpetuation. (C)) 2007 Elsevier Inc. All rights reserved.. ...
Therapy of lymphomas with immunotoxins comprised of soluble T-cell receptors conjugated to ricin A-chain.. Immunotoxins are conjugates of cell-binding moieties linked to toxins or toxin subunits. Many investigators have used antibodies directed against tumor specific-antigens as the cell- binding moiety. B-cell tumors are ideal for this type of therapy because of their tumor-specific idiotype (Id). However, B-cell tumors often secrete Id which competes with tumor cells for binding of anti-Id antibodies. Therefore, to overcome this obstacle, my research focuses on developing soluble T-cell receptors (TCR) from tumor specific T cell clones as the cell-binding moiety, and ricin-A chain as the toxic moiety. T-cells recognize cell-bound (but not soluble) antigen in association with products of the major histocompatibility complex. Therefore, free idiotype should not interfere with binding of soluble TCRs to tumor cells. Id specific T-cells have been cloned from mice immunized with the murine tumor, ...
Progesterone Receptor antibody [C262], C-term (progesterone receptor) for IP, WB. Anti-Progesterone Receptor mAb (GTX80350) is tested in Human, Mouse, Rat, Rabbit samples. 100% Ab-Assurance.
Antibodies, Treatment, Human, Patients, Plasma, Serum, Igg, Immunoglobulin, Monoclonal Antibodies, Therapeutic, Anti-idiotype Antibodies, Breast, Breast Cancer, Calibration, Cancer, Elisa, Enzyme-linked Immunosorbent Assay, Horseradish, Horseradish Peroxidase, Immunoassay
T cell vaccination (TCV) activates Tregs of 2 kinds: anti-idiotypic (anti-id) and anti-ergotypic (anti-erg). These regulators furnish a useful view of the physiology of T cell regulation of the immune response. Anti-id Tregs recognize specific effector clones by their unique TCR CDR3 peptides; anti-id networks of CD4+ and CD8+ Tregs have been described in detail. Here we shall focus on anti-erg T regulators. Anti-erg T cells, unlike anti-id T cells, do not recognize the clonal identity of effector T cells; rather, anti-erg T cells recognize the state of activation of target effector T cells, irrespective of their TCR specificity. We consider several features of anti-erg T cells: their ontogeny, subset markers, and target ergotope molecules; mechanisms by which they regulate other T cells; mechanisms by which they get regulated; and therapeutic prospects for anti-erg upregulation and downregulation.. ...
The basic components of the diagnostic test systems are antigens and specific antibodies. The main objective of developing express tests for the diagnosis of bovine leukemia virus (BLV) is to obtain a virus antigen drug, which is very time-consuming to prepare. This problem can be solved by producing anti-idiotype antibodies that have a chemical structure identical to that of the viral antigen and does not require large expenditures to manufacture [1, 2 ...
Rabbit recombinant monoclonal Progesterone Receptor antibody [SP2] validated for IHC and tested in Human. Immunogen corresponding to recombinant full length…
|strong|Human anti Cetuximab, clone AbD19834_IgG1|/strong|, is an anti-idiotypic antibody that binds specifically to cetuximab and inhibits the binding of the drug to its target, epidermal growth fact…
|strong|Human anti golimumab, clone AbD20692_hIgG1|/strong| is a Type 1 anti-idiotypic antibody that specifically recognizes free golimumab but not the drug/tumor necrosis factor alpha (TNFα) co…
Matusevich, O V; Egorov, V V; Gluzdikov, I A; Titov, M I; Shtro, A A; Slita, A V; Dukov, M I; Shurygina, A-P S; Smirnova, T D; Kudryavtsev, I V; Vasin, A V; Kiselev, O I; Zarubaev, Vladimir V. ...
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Barusa resident is the male avi modeling in my photos with me. Thank you Barusa for your help, your patience, and tolerating me ❨ Need mens stuff blogged, please contact. ...
成會明。2004。奈米碳管。五南出版社。 韋進全、張先鋒、王坤林著、楊明勳校訂。2009。奈米碳管巨觀體。五南出版社。 國立科學工藝博物館網站 http://nano.nstm.gov.tw。 黃建盛。2006。奈米碳管簡介。科學新天地第十三期。 黃啟仁。2008。哺乳動物及家禽免疫細胞作為益生菌調控免疫機制於體外模式之探討。碩士論文。中興大學。台中。 葉晨聖、曾 厚、張佑民、何佳安、黃世宏、李明威、陳志宏、方偉峰、孫啟光、王國禎、張立惠、黃暄益、吳信毅、陳東煌、吳炳慶、徐瑋玲、李國賓、黃志嘉、鄭豐裕、陳昭瑜、盧陽明、張富雄、廖韋晴、楊宗翰、陳敏慧、楊智强、謝達斌、楊鏡堂、蘇家豪、蕭俊龍。2007。教育部「生物及醫學科技人才培育先導型計畫」生醫奈米技術。 劉吉平、郝向陽。2003。奈米科學與技術。世茂出版社。 ...
This cell then secretes the unique idiotype, or immunoglobulin antigen characteristic of the individual tumor, which is ...
ROR-1 and the immunoglobulin light-chain idiotype expressed by the individual cancer. CARs targeting either CD33 or CD123 have ... Toxicity against CD19 results in B cell loss in circulation and in bone marrow that can be overcome by periodic immunoglobulin ... Loss of B cells can be countered with immunoglobulin. Startups including Juno Therapeutics exploit the combination of ...
... immunoglobulin idiotypes MeSH D12.776.124.790.651.705.500 - immunoglobulin heavy chains MeSH D12.776.124.790.651.705.500.350 - ... immunoglobulin constant regions MeSH D12.776.124.486.485.680.745 - immunoglobulin idiotypes MeSH D12.776.124.486.485.705 - ... immunoglobulin a MeSH D12.776.124.486.485.114.619.026.030 - immunoglobulin a, secretory MeSH D12.776.124.486.485.114.619.026. ... immunoglobulin d MeSH D12.776.124.486.485.114.619.251.500 - immunoglobulin delta-chains MeSH D12.776.124.486.485.114.619.312 - ...
... immunoglobulin idiotypes MeSH D23.050.705.115 - antigens, human platelet MeSH D23.050.705.230 - blood group antigens MeSH ...
So, when a full, high-fidelity copy of the idiotype is linked to a foreign protein (KLH), and administered with an immune- ... manufactured with an IgG isotype with each treatment vaccine produced to correspond with the patient's tumor immunoglobulin ... "Idiotype vaccine therapy (BiovaxID) in follicular lymphoma in first complete remission: Phase III clinical trial results". ... This approach is used because there is a unique protein called an "idiotype" expressed exclusively on the cancerous B-cells. ...
Immunoglobulins or TCRs with a shared idiotope are the same idiotype. Antibody idiotype is determined by: Gene rearrangement ... In immunology, an idiotype is a shared characteristic between a group of immunoglobulin or T-cell receptor (TCR) molecules ... Allotype (immunology) Isotype (immunology) Immune network theory Immunoglobulin+idiotypes at the US National Library of ... idiotype describes the distinctive sequence and region that makes any immunoglobulin/TCR unique from others of the same type ...
Idiotype cross-reaction - Idiotypes are antigenic epitopes found in the antigen-binding portion (Fab) of the immunoglobulin ... Idiotype network theory, proposed by Jerne, wherein a network of antibodies capable of neutralizing self-reactive antibodies ... In this case, the host-cell receptor is envisioned as an internal image of the virus, and the anti-idiotype antibodies can ... Plotz and Oldstone presented evidence that autoimmunity can arise as a result of a cross-reaction between the idiotype on an ...
... (IgE) is a type of antibody (or immunoglobulin (Ig) "isotype") that has only been found in mammals. IgE is ... Presence of a unique immunoglobulin as a carrier of reaginic activity". J. Immunol. 97 (1): 75-85. PMID 4162440.. ... Winter WE, Hardt NS, Fuhrman S (2000). "Immunoglobulin E: importance in parasitic infections and hypersensitivity responses". ... Johansson SG, Bennich H. Immunological studies of an atypical (myeloma) immunoglobulin. Immunology 1967; 13:381-94. ...
Immunoglobulin allotype) · ஒரினவகை (Isotype) · தன்வகை (idiotype) · நோயெதிர்ப்பிகளின் தொகுதி (Immune complex) · Paratope · ... Immunoglobulin class switching) · முக்கிய திசுப்பொருத்தக் கூட்டமைவு (MHC)/மனித வெள்ளையணு எதிர்ப்பி (HLA) · ...
Immunoglobulin allotype) · ஒரினவகை (Isotype) · தன்வகை (idiotype) · நோயெதிர்ப்பிகளின் தொகுதி (Immune complex) · Paratope · ... Immunoglobulin class switching) · முக்கிய திசுப்பொருத்தக் கூட்டமைவு (MHC)/மனித வெள்ளையணு எதிர்ப்பி (HLA) · ... killing cell Immunoglobulin Receptors) (KIR) மூலமாக அங்கீகரிக்கப்படுகின்றன, அதன் அடிப்படையில் கொல்லும் செல்களில் தடைகளை ...
The structure of immunoglobulin polypeptide chain is dictated and controlled by number of genes encoded in the germ line. ... Allotype (disambiguation) Idiotype Isotype "Pathology, Microbiology and Immunology". University of South Carolina School of ... Immunoglobulin+allotypes at the US National Library of Medicine Medical Subject Headings (MeSH) Overview at University of South ... However, it is important to mention that not all variations in immunoglobulin amino acid sequence pose as a determinant ...
Immunoglobulin lambda joining 3 is a protein that in humans is encoded by the IGLJ3 gene. "Human PubMed Reference:". National ... "The anti-DNA-associated idiotype 8.12 is encoded by the V lambda II gene family and maps to the vicinity of L chain CDR1". ... Combriato G, Klobeck HG (June 1991). "V lambda and J lambda-C lambda gene segments of the human immunoglobulin lambda light ... "Entrez Gene: Immunoglobulin lambda joining 3". Paul E, Iliev AA, Livneh A, Diamond B (December 1992). " ...
Idiotype Janeway, CA; Travers, P; Walport, M; et al. (2001). "Immunobiology: The Immune System in Health and Disease. 5th ... Immunoglobulin+Isotypes at the US National Library of Medicine Medical Subject Headings (MeSH) Overview at University of South ... In immunology, antibodies (immunoglobulins (Ig)) are classified into several types called isotypes or classes. The variable (V ... Stavnezer, Janet (1996). "Immunoglobulin Class Switching". Current Opinion in Immunology. 8 (2): 199-205. doi:10.1016/s0952- ...
IgA1 IgA2 Immunoglobulin D (IgD) Immunoglobulin E (IgE) Immunoglobulin G (IgG) IgG1 IgG2 IgG3 IgG4 Immunoglobulin M (IgM) ... antibodies Polyclonal antibodies Autoantibody Microantibody Neutralizing antibody Classification Allotype Isotype Idiotype ... Immunoglobulin heavy chain / Immunoglobulin light chain Co-receptors Stimulatory CD21 CD19 CD81 Inhibitory CD22 Accessory ... IL10RB Immunoglobulin superfamily (Some members) CSF1 CD117 (c-KIT) IL1 receptor family (IL1R) IL1R type 1 (CD121a) IL1R type 2 ...
D Immunoglobulin E Immunoglobulin G Immunoglobulin heavy chain Immunoglobulin light chain Immunoglobulin M Immunoglobulin Y ... Human leukocyte antigen Humoral immunity Hybridoma technology Hyperimmune globulin Hypersensitive response Idiotopes Idiotype ... Immunofixation Immunogen Immunogenetics Immunogenicity Immunoglobulin A Immunoglobulin class switching Immunoglobulin ...
... immunoglobulin constant regions MeSH D12.776.377.715.548.680.745 - immunoglobulin idiotypes MeSH D12.776.377.715.548.705 - ... immunoglobulin a MeSH D12.776.377.715.548.114.619.026.030 - immunoglobulin a, secretory MeSH D12.776.377.715.548.114.619.026. ... immunoglobulin d MeSH D12.776.377.715.548.114.619.251.500 - immunoglobulin delta-chains MeSH D12.776.377.715.548.114.619.312 - ... immunoglobulin g MeSH D12.776.377.715.548.114.619.393.522 - immunoglobulin gamma-chains MeSH D12.776.377.715.548.114.619.393. ...
Immunoglobulin-binding protein - Proteins such as protein A, protein G, and protein L that are capable of binding to antibodies ... Different antibody idiotypes, each have distinctly formed complementarity-determining regions. Allergen - A substance capable ... While antigens are the "target" of antibodies, immunoglobulin-binding proteins "attack" antibodies. T-dependent antigen - ...
Wilson, P. C.; Wilson, K.; Liu, Y. J.; Banchereau, J.; Pascual, V.; Capra, J. D. (2000). "Receptor revision of immunoglobulin ... Evidence that the VH4-21 gene segment is responsible for the major cross-reactive idiotype". Journal of Immunology. 146 (12): ... Hasemann, C. A.; Capra, J. D. (1990). "High-level production of a functional immunoglobulin heterodimer in a baculovirus ... Pascual, V.; Capra, J. D. (1991). Human immunoglobulin heavy-chain variable region genes: Organization, polymorphism, and ...
Somatic recombination of immunoglobulins, also known as V(D)J recombination, involves the generation of a unique immunoglobulin ... According to immune network theory, the adaptive immune system is regulated by interactions between idiotypes. ... The membrane-bound form of an antibody may be called a surface immunoglobulin (sIg) or a membrane immunoglobulin (mIg). It is ... Further information: Immunoglobulin light chain. In mammals there are two types of immunoglobulin light chain, which are called ...
Intravenous immunoglobulin (IVIG) was used successfully in an infant with IgA-associated autoimmune hemolytic anemia. ... with a distinct idiotype identified by the 9G4 rat murine monoclonal antibody. AIHA can be classified as warm autoimmune ...
In the early 1990s, Bogen and co-workers discovered a new type of collaboration between T and B-cells (Idiotype-driven T-B ... Mar;22(3):371-83.PMID 15780993 Weiss S, Bogen B. (1989) B-lymphoma cells process and present their endogenous immunoglobulin to ... Epub 2007 May 7. PMID 17485509 Lauritzsen GF, Weiss S, Dembic Z, Bogen B. (1994) Naive idiotype-specific CD4+ T cells and ... PMID 10706628 Fredriksen AB, Bogen B. (2007) Chemokine-idiotype fusion DNA vaccines are potentiated by bivalency and xenogeneic ...
Somatic recombination of immunoglobulins, also known as V(D)J recombination, involves the generation of a unique immunoglobulin ... According to immune network theory, the adaptive immune system is regulated by interactions between idiotypes. ... The membrane-bound form of an antibody may be called a surface immunoglobulin (sIg) or a membrane immunoglobulin (mIg). It is ... For more details on this topic, see Immunoglobulin light chain.. In mammals there are two types of immunoglobulin light chain, ...
Robertson, Miranda (1981). "Genes of lymphocytes II: T-cell receptors, idiotypes and the MHC". Nature. 291 (5812): 187-188. doi ... Robertson, Miranda (1983). "Tumours of lymphocytes: What happens when cellular oncogenes collide with immunoglobulin genes". ...
Yewdell J (4 October 2003). "He put the Id in Idiotype". EMBO Reports (Book review). 4 (10): 931. doi:10.1038/sj.embor.embor951 ... Many of the classical molecules of the adaptive immune system (for example, immunoglobulins and T-cell receptors) exist only in ... Saji F, Samejima Y, Kamiura S, Koyama M (May 1999). "Dynamics of immunoglobulins at the feto-maternal interface" (PDF). Reviews ... Housden NG, Harrison S, Roberts SE, Beckingham JA, Graille M, Stura E, Gore MG (June 2003). "Immunoglobulin-binding domains: ...
... s can only produce a single kind of antibody in a single class of immunoglobulin. In other words, every B cell is ... Primary amyloidosis (AL) is caused by the deposition of excess immunoglobulin light chains which are secreted from plasma cells ... Educational Resource on the Biology of Immunoglobulins. *Histology image: 21001loa - Histology Learning System at Boston ... and do not take up antigen because they no longer display significant quantities of immunoglobulin on the cell surface.[4] ...
... anti-idiotypes' (antigen receptors that react with the idiotype as if it were a foreign antigen). This theory, which builds on ... Antibodies (also known as immunoglobulin, Ig), are large Y-shaped proteins used by the immune system to identify and neutralize ... In antibody responses, B cells are activated to secrete antibodies, which are proteins also known as immunoglobulins. ... Dong Y, Taylor HE, Dimopoulos G (July 2006). "AgDscam, a hypervariable immunoglobulin domain-containing receptor of the ...
Immunoglobulins are glycoproteins in the immunoglobulin superfamily that function as antibodies. The terms antibody and ... structure (1972), immunoglobulin genes (1976). Founder: P Ehrlich[2] Mechanism [11][edit]. In humoral immune response, first ... Each immunoglobulin class differs in its biological properties and has evolved to deal with different antigens.[12] Antibodies ... immunoglobulin are often used interchangeably. They are found in the blood and tissue fluids, as well as many secretions. In ...
... anti-idiotype vaccine - anti-inflammatory - antiandrogen - antiandrogen therapy - anti-angiogenesis - antiangiogenic - antibody ... immunoglobulin - immunological adjuvant - immunology - immunomodulation - immunophenotyping - immunoscintigraphy - ...
Immunoglobulin allotype) · ஒரினவகை (Isotype) · தன்வகை (idiotype) · நோயெதிர்ப்பிகளின் தொகுதி (Immune complex) · Paratope · ... Immunoglobulin class switching) · முக்கிய திசுப்பொருத்தக் கூட்டமைவு (MHC)/மனித வெள்ளையணு எதிர்ப்பி (HLA) · ...
... s (also called leukocytes or leucocytes and abbreviated as WBCs) are the cells of the immune system that are involved in protecting the body against both infectious disease and foreign invaders. All white blood cells are produced and derived from multipotent cells in the bone marrow known as hematopoietic stem cells. Leukocytes are found throughout the body, including the blood and lymphatic system.[1] All white blood cells have nuclei, which distinguishes them from the other blood cells, the anucleated red blood cells (RBCs) and platelets. Types of white blood cells can be classified in standard ways. Two pairs of broadest categories classify them either by structure (granulocytes or agranulocytes) or by cell lineage (myeloid cells or lymphoid cells). These broadest categories can be further divided into the five main types: neutrophils, eosinophils (acidophiles), basophils, lymphocytes, and monocytes.[2] These types are distinguished by their physical and functional ...
TI-1 antigens have an intrinsic B cell activating activity, that can directly cause proliferation and differentiation of B lymphocytes without T cell stimulation and independently of their BCR specificity. TI-1 antigens activate B-cells via Toll like receptors, which are, in human, expressed on the surface of B lymphocytes after BCR stimulation. TI-1 antigens are classified as B-cell mitogens, because they induce numerous cell divisions. In higher concentrations, TI-1 antigens bind to BCR and TLR of various clones of B lymphocytes, which leads to production of multiclonal antibodies. But when the concentration of TI-1 is lower, it can activate only B lymphocytes with specific binding of TI-1 on their BCR, and leads to production of monoclonal antibodies.[1] This part of immune response may be important in some early stages of infection by extracellular pathogens, because it is rapidly activated and does not require T cell help or clonal maturation and expansion. An example of TI-1 antigen is ...
Neutrophils are normally found in the bloodstream and are the most abundant type of phagocyte, constituting 60% to 65% of the total circulating white blood cells.[4] One litre of human blood contains about five billion (5x109) neutrophils,[5] which are about 12-15 micrometers in diameter.[6] Once neutrophils have received the appropriate signals, it takes them about thirty minutes to leave the blood and reach the site of an infection.[7] Neutrophils do not return to the blood; they turn into pus cells and die.[7] Mature neutrophils are smaller than monocytes, and have a segmented nucleus with several sections(two to five segments); each section is connected by chromatin filaments. Neutrophils do not normally exit the bone marrow until maturity, but during an infection neutrophil precursors called myelocytes and promyelocytes are released.[8]. Neutrophils have three strategies for directly attacking micro-organisms: phagocytosis (ingestion), release of soluble anti-microbials (including granule ...
Idiotype Cross-Reaction - Idiotypes are antigenic epitopes found in the antigen-binding portion (Fab) of the immunoglobulin ... Idiotype Network theory, proposed by Jerne, wherein a network of antibodies capable of neutralizing self-reactive antibodies ... In this case, the host-cell receptor is envisioned as an internal image of the virus, and the anti-idiotype antibodies can ... Plotz and Oldstone presented evidence that autoimmunity can arise as a result of a cross-reaction between the idiotype on an ...
... anti-idiotypes' (antigen receptors that react with the idiotype as if it were a foreign antigen). This theory, which builds on ... Antibodies (also known as immunoglobulin, Ig), are large Y-shaped proteins used by the immune system to identify and neutralize ... In antibody responses, B cells are activated to secrete antibodies, which are proteins also known as immunoglobulins. ... based on interactions between idiotypes (unique molecular features of one clonotype, i.e. the unique set of antigenic ...
In the early 1950s Immunoglobulin(Ig) was used by doctors to treat patients with primary immunodeficiency through intramuscular ...
Killer-cell immunoglobulin-like receptors (KIRs) belong to a multigene family of more recently evolved Ig-like extracellular ... ILT or LIR (immunoglobulin-like receptor) - are recently discovered members of the Ig receptor family. ... NCR (natural cytotoxicity receptors), a type of type 1 transmembrane proteins of the immunoglobulin superfamily, upon ... or pan T marker CD3 or surface immunoglobulins (Ig) B cell receptors, but they usually express the surface markers CD16 (Fc ...
A defect in this gene results in an inability to undergo immunoglobulin class switching and is associated with hyper IgM ...
... s are phagocytes, capable of ingesting microorganisms or particles. For targets to be recognized, they must be coated in opsonins-a process known as antibody opsonization.[15] They can internalize and kill many microbes, each phagocytic event resulting in the formation of a phagosome into which reactive oxygen species and hydrolytic enzymes are secreted. The consumption of oxygen during the generation of reactive oxygen species has been termed the "respiratory burst", although unrelated to respiration or energy production. The respiratory burst involves the activation of the enzyme NADPH oxidase, which produces large quantities of superoxide, a reactive oxygen species. Superoxide decays spontaneously or is broken down via enzymes known as superoxide dismutases (Cu/ZnSOD and MnSOD), to hydrogen peroxide, which is then converted to hypochlorous acid (HClO), by the green heme enzyme myeloperoxidase. It is thought that the bactericidal properties of HClO are enough to kill bacteria ...
Different virus families have different levels of ability to alter their genomes and trick the immune system into not recognizing. Some viruses have relatively unchanging genomes like paramyxoviruses while others like influenza have rapidly changing genomes that inhibit our ability to create long lasting vaccines against the disease. Viruses in general have much faster rate of mutation of their genomes than human or bacterial cells. In general viruses with shorter genomes have faster rates of mutation than longer genomes since they have a faster rate of replication.[15] It was classically thought that viruses with an RNA genome always had a faster rate of antigenic variation than those with a DNA genome because RNA polymerase lacks a mechanism for checking for mistakes in translation but recent work by Duffy et al. shows that some DNA viruses have the same high rates of antigenic variation as their RNA counterparts.[15] Antigenic variation within viruses can be categories into 6 different ...
This change in shape allows the binding of plasma protein Factor B, which allows Factor D to cleave Factor B into Ba and Bb. Bb remains bound to C3(H2O) to form C3(H2O)Bb. This complex is also known as a fluid-phase C3-convertase. This convertase, the alternative pathway C3-convertase, although only produced in small amounts, can cleave multiple C3 proteins into C3a and C3b. The complex is believed to be unstable until it binds properdin, a serum protein. The addition of properdin forms the complex C3bBbP, a stable compound which can bind an additional C3b to form alternative pathway C5-convertase. The C5-convertase of the alternative pathway consists of (C3b)2BbP (sometimes referred to as C3b2Bb). After the creation of C5 convertase (either as (C3b)2BbP or C4b2a3b from the classical pathway), the complement system follows the same path regardless of the means of activation (alternative, classical, or lectin). C5-convertase cleaves C5 into C5a and C5b. C5b binds sequentially to C6, C7, C8 and ...
Central tolerance refers to the tolerance established by deleting autoreactive lymphocyte clones before they develop into fully immunocompetent cells. It occurs during lymphocyte development in the thymus[14][15] and bone marrow for T and B lymphocytes, respectively. In these tissues, maturing lymphocytes are exposed to self-antigens presented by medullary thymic epithelial cells and thymic dendritic cells, or bone marrow cells. Self-antigens are present due to endogenous expression, importation of antigen from peripheral sites via circulating blood, and in the case of thymic stromal cells, expression of proteins of other non-thymic tissues by the action of the transcription factor AIRE. Those lymphocytes that have receptors that bind strongly to self-antigens are removed by induction of apoptosis of the autoreactive cells, or by induction of anergy, a state of non-activity.[16] Weakly autoreactive B cells may also remain in a state of immunological ignorance where they simply do not respond to ...
... s have protein receptors on their cell surface that bind IgE, an immunoglobulin involved in macroparasite defense and ...
Activation of CD4+ T cells occurs through the simultaneous engagement of the T-cell receptor and a co-stimulatory molecule (like CD28, or ICOS) on the T cell by the major histocompatibility complex (MHCII) peptide and co-stimulatory molecules on the APC. Both are required for production of an effective immune response; in the absence of co-stimulation, T cell receptor signalling alone results in anergy. The signalling pathways downstream from co-stimulatory molecules usually engages the PI3K pathway generating PIP3 at the plasma membrane and recruiting PH domain containing signaling molecules like PDK1 that are essential for the activation of PKCθ, and eventual IL-2 production. Optimal CD8+ T cell response relies on CD4+ signalling.[39] CD4+ cells are useful in the initial antigenic activation of naïve CD8 T cells, and sustaining memory CD8+ T cells in the aftermath of an acute infection. Therefore, activation of CD4+ T cells can be beneficial to the action of CD8+ T cells.[40][41][42] The ...
"Organization and Expression of Immunoglobulin Genes (Chapter 5)". Immunology (Fifth ed.). New York. pp. 105-136. ISBN 0-7167- ... Monoclonal antibodies are structurally identical immunoglobulin molecules with identical epitope-specificity (all of them bind ...
Another laboratory test is the blood test for IgE (immunoglobulin production), such as the radioallergosorbent test (RAST) or ... are required for immunoglobulin E binding and activation of effector cells from allergic patients". FASEB Journal. 17 (12): ... Most humans mount significant Immunoglobulin E responses only as a defense against parasitic infections. However, some ... is an antigen that is capable of stimulating a type-I hypersensitivity reaction in atopic individuals through Immunoglobulin E ...
Immunoglobulins. Antibodies. QS 21. Immunoglobulin Idiotypes. Immunologic Factors. Physiological Effects of Drugs. Adjuvants, ... The idiotype of the immunoglobulin on a given B cell malignancy (Id) can serve as a clonal marker, and a previous pilot study ... Vaccination of Follicular Lymphomas With Tumor-Derived Immunoglobulin Idiotype. The safety and scientific validity of this ... Induction of immune responses in patients with B-cell lymphoma against the surface-immunoglobulin idiotype expressed by their ...
The idiotype of the immunoglobulin on a given B cell malignancy (Id) can serve as a clonal marker, and a previous pilot study ... The idiotype of the immunoglobulin on a given B cell malignancy (Id) can serve as a clonal. marker, and a previous pilot study ... Active Specific Immunotherapy for Follicular Lymphomas With Tumor-Derived Immunoglobulin Idiotype Antigen Vaccines. Trial Phase ... against the unique idiotype of the patients lymphoma and 2) to evaluate the ability of the. Id vaccine to clear the bone ...
"Immunoglobulin Idiotypes" by people in UAMS Profiles by year, and whether "Immunoglobulin Idiotypes" was a major or minor topic ... "Immunoglobulin Idiotypes" is a descriptor in the National Library of Medicines controlled vocabulary thesaurus, MeSH (Medical ... Below are the most recent publications written about "Immunoglobulin Idiotypes" by people in Profiles over the past ten years. ... Below are MeSH descriptors whose meaning is more general than "Immunoglobulin Idiotypes". ...
keywords = "Anti-idiotype, Atherosclerosis, Idiotype, Intravenous immunoglobulin, Oxidized low-density lipoprotein",. author ... title = "Anti-idiotypes to oxidized LDL antibodies in intravenous immunoglobulin preparations - Possible immunomodulation of ... T1 - Anti-idiotypes to oxidized LDL antibodies in intravenous immunoglobulin preparations - Possible immunomodulation of ... Anti-idiotypes to oxidized LDL antibodies in intravenous immunoglobulin preparations - Possible immunomodulation of ...
Immunoglobulin idiotypes; experimental and clinical applications. Immunoglobulin idiotypes; experimental and clinical ... Immunoglobulin Idiotypes , Kidney Tubules , Multiple Sclerosis/immunology , Neoplasms/immunology , Receptors, Cell Surface/ ...
A study has been made of the presence of the major rheumatoid Factor cross-reacting idiotype (RCRI) in the serum of patients ... 0/Immunoglobulin Idiotypes; 0/Pokeweed Mitogens; 9009-79-4/Rheumatoid Factor From MEDLINE®/PubMed®, a database of the U.S. ... Immunoglobulin Idiotypes / immunology*. Lymphocyte Activation. Male. Middle Aged. Pokeweed Mitogens / pharmacology. Rheumatoid ... A study has been made of the presence of the major rheumatoid Factor cross-reacting idiotype (RCRI) in the serum of patients ...
Pilot Study of Idiotype Vaccine and EPOCH-Rituximab Chemotherapy in Untreated Mantle Cell Lymphoma. ... To assess if EPOCH-R/idiotype vaccination is associated with a median progression-free survival consistent with 36 months; ... These results suggest that idiotype vaccines may be able to induce a clinically significant immune response against lymphoma. ... In the present study, we propose to investigate the efficacy of idiotype vaccine treatment in previously untreated patients ...
Biological: Follicular lymphoma, patient-specific, soluble protein idiotype vaccine 0.5 mg of idiotype conjugated to 0.5 mg of ... that is the capacity of inducing an idiotype- and tumor-specific immune response (Kwak LW et al. NEJM 1992); clinical efficacy ...
IDIOTYPE-ANTI-IDIOTYPE THEORY IN THE DETERMINATION OF ABNORMAL IM-MUNOGLOBULIN (aIgG) IN SERA OF PATIENTS WITH GRAVES DISEASE ... IDIOTYPE-ANTI-IDIOTYPE THEORY IN THE DETERMINATION OF ABNORMAL IM-MUNOGLOBULIN (aIgG) IN S ... a new method for detecting the abnormal immunoglobulin (aIgG) in the sera of patients with Graves disease (GD) was reported. In ...
Inherited immunoglobulin idiotypes of the mouse. Immunol. Rev. 34: 119. OpenUrlCrossRefPubMed ... The half-lives of serum immunoglobulins in adult mice. Eur. J. Immunol. 18: 313. ...
8. Idiotypes. Serological term--antigenicdeterminant unique to a given immunoglobulin.Used to describe the ... 4. 1. Antigenic determinants used to describe parts of immunoglobulins: isotypes and idiotypes.2. Immunological tests: ... Those rabbitantibodies recognize isotypic determinants,and will react with immunoglobulins in theserum of virtually all humans ... 1. Antigenic determinants onimmunoglobulins: isotypes and idiotypes 2. Immunoelectrophoresis, agglutination,radioimmunoassay, ...
Clonal deletion of specific thymocytes by an immunoglobulin idiotype. EMBO J. 12, 357-363 (1993).. ... Deletion of CD4+ T cells specific for a monoclonal immunoglobulin idiotype secreted by a plasmacytoma. Eur. J. Immunol. 26, ... Idiotype-specific Th cells support oligoclonal expansion of anti-dsDNA B cells in mice with lupus. J. Immunol. 193, 2691-2698 ( ... BCR ligation is required for idiotype-driven T-B collaboration in the spleen. (A) Experimental setup (Id-sp. T/anti-Id IgG, n ...
Recognition of immunoglobulin idiotypes by thymus-derived lymphocytes. Proc Natl Acad Sci U S A 1975; 72: 2357-60. ... including the presentation of epitopes derived from their own unique immunoglobulin idiotype to CD4+ and CD8+ T cells ( 5- 7). ... The clonotypic idiotype of malignant B cells was the first tumor-specific antigen identified as capable of eliciting a T cell- ... Lundin KU, Hofgaard PO, Omholt H, Munthe LA, Corthay A, Bogen B. Therapeutic effect of idiotype-specific CD4+ T cells against B ...
We examined host responses against ART-18 idiotype (Id) and mouse immunoglobulin in recipients immunomodulated with ART-18 mAb ... Pretreatment with cyclosporine and anti-interleukin 2 receptor antibody abrogates the anti-idiotype response in rat recipients ... Pretreatment with cyclosporine and anti-interleukin 2 receptor antibody abrogates the anti-idiotype response in rat recipients ... Pretreatment with cyclosporine and anti-interleukin 2 receptor antibody abrogates the anti-idiotype response in rat recipients ...
1977) Inherited immunoglobulin idiotypes of the mouse. Immunol. Rev. 34:119-138, pmid:66781. ... 1996) Somatic mutation of immunoglobulin V genes in vitro. Science. 271:1285-1289, pmid:8638111. ... 1981) The bone marrowthe major source of serum immunoglobulins, but still a neglected site of antibody formation. Clin. Exp. ... 1994) Analysis of individual immunoglobulin lambda light chain genes amplified from single cells is inconsistent with variable ...
The unique immunoglobulin (Ig) idiotype on the surface of each B-cell August 7, 2019. Published by: antibody ... The unique immunoglobulin (Ig) idiotype on the surface of each B-cell lymphoma represents an ideal tumor-specific antigen for ... randomized clinical tests are currently under way to determine the effectiveness of idiotype vaccination in individuals with ...
USA, 93:7252-7257 (1996)); idiotypic epitopes or antigens, for example, immunoglobulin idiotypes or T cell receptor idiotypes ...
There is provided an isolated immunoglobulin comprising two heavy polypeptide chains sufficient for the formation of a complete ... Such anti-idiotypes can be produced against human or animal idiotypes. A property of these anti-idiotypes is that they can be ... The invention also relates to anti-idiotypes capable of recognizing idiotypes of heavy-chain immunoglobulins. ... The immunoglobulins of the type described here-above can comprise type G immunoglobulins and especially immunoglobulins which ...
Anti-idiotypes to oxidized LDL antibodies in intravenous immunoglobulin preparations--possible immunomodulation of ...
The role of idiotype and the MHC in suppressor T cell pathways, in: Immunoglobulin Idiotypes: ICN-UCLA Symposia on Molecular ... Idiotype-anti-idiotype network. II. Activation of silent clones by treatment at birth with idiotypes is associated with the ... Maternal Environment Receptor Repertoire Limit Dilution Analysis Antibody Variable Region Immunoglobulin Idiotype ... Woodland, R., and Cantor, N., 1978, Idiotype-specific T helper cells are required to induce idiotype-positive B memory cells to ...
Anti-idiotypes oxidized LDL antibodies in intravenous immunoglobulin preparations: possible immunomodulation of atherosclerosis ... The reduced atherosclerosis correlated with the levels of immunoglobulin G (IgG) anti-oxLDL antibodies.11,42-45 Transfer of B ... Immunoglobulin treatment reduces atherosclerosis in apo E knockout mice. J Clin Invest. 1998; 102: 910-918. ... Immunoglobulin M type of autoantibodies to oxidized low-density lipoprotein has an inverse relation to carotid artery ...
Clonal deletion of specific thymocytes by an immunoglobulin idiotype. EMBO J. 12: 357-363. ... Immunoglobulin-specific T-B cell interaction. IV. B cell presentation of idiotypic determinant(s) of monoclonal anti-surface ... Activation and tolerance in CD4(+) T cells reactive to an immunoglobulin variable region. J. Exp. Med. 200: 1-11. ... Hybridoma proteins expressing the predominant idiotype of the antiazophenylarsonate response of A/J mice. Proc. Natl. Acad. Sci ...
The unique immunoglobulin (Ig) idiotype on the surface of each B-cell. The unique immunoglobulin (Ig) idiotype on the surface ...
Anti-idiotype sera raised against surface immunoglobulin of human neoplastic lymphocytes. J Exp Med (1976) 144(4):960-9. doi: ... Preliminary experience in treating lymphocytic leukaemia with antibody to immunoglobulin idiotypes on the cell surfaces. Br J ... Induction of immune responses in patients with B-cell lymphoma against the surface-immunoglobulin idiotype expressed by their ... Miller RA, Maloney DG, Warnke R, Levy R. Treatment of B-cell lymphoma with monoclonal anti-idiotype antibody. N Engl J Med ( ...
Immunoglobulin deficiency and idiotype expression in children developing Haemophilus influenzae type b disease after ... subnormal immunoglobulin concentrations were present in approximately 40% of those who developed invasive Hib disease (33). ... had evidence of low immunoglobulin levels (34). COMBINATION VACCINES TETRAMUNETM ...
... immunoregulation of immunoglobulin synthesis by an allotype-idiotype network. ... A bi-directional immune network mechanism: simultaneous induction of idiotype and anti-anti-idiotype in rabbits immunized with ... The common idiotypic specificity of anti-a2 allotype antibodies: contribution of H and L chains to idiotype expression. ... A common idiotypic specificity of rabbit antibodies to the a2 allotype of the immunoglobulin heavy chain variable region. ...
Isenberg DA, Collins C: Detection of cross-reactive anti-DNA antibody idiotypes on renal tissue-bound immunoglobulins from ... Koffler D, Agnello V, Carr I, et al.: Variable patterns of immunoglobulin and complement deposition in the kidneys of patients ... Blaese M, Grayson J, Steinberg AD: Increased immunoglobulin-secreting cells in the blood of patients with active systemic lupus ...
The unique immunoglobulin idiotype expressed on the surface of B lymphoma cells can be used as an effective antigen in tumor- ... The unique immunoglobulin (Ig) idiotype on the surface of each B-cell lymphoma represents an ideal tumor-specific antigen for ... IDIOTYPE VACCINATION AGAINST MURINE B-CELL LYMPHOMA - HUMORAL AND CELLULAR-RESPONSES ELICITED BY TUMOR-DERIVED IMMUNOGLOBULIN-M ... Mice vaccinated with the affinity-purified 38C13 scFv generate >10 micrograms/ml anti-idiotype immunoglobulins. These mice were ...
Recombinant hisitidine-tagged idiotype immunoglobulin Fab fragment of clonal B-cell receptors ... Recombinant histidine-tagged idiotype immunoglobulin Fab fragment of clonal B-cell receptors ... Recombinant histidine-tagged idiotype immunoglobulin Fab fragment of clonal B-cell receptors ... Recombinant histidine-tagged idiotype immunoglobulin Fab fragment of clonal B-cell receptors ...
  • Objective: The aim of this study was to examine whether intravenous immunoglobulin (IVIg) preparations contain anti-oxLDL and anti-anti-oxLDL antibodies. (elsevier.com)
  • Absorption assays were carried out to evaluate the presence of anti-idiotypes against anti-oxLDL antibodies in IVIg preparations. (elsevier.com)
  • To investigate the presence of anti-idiotypes against anti-oxLDL antibodies within IVIg, F(ab′) 2 fragments of IVIg IgG were used to absorb IgG F(ab′) 2 fragments from the pool of sera with high anti-oxLDL levels. (elsevier.com)
  • Monoclonal anti-idiotypic (anti-Id) antibodies (Ab2) were generated against idiotypes (Id) of canine parvovirus (CPV) specific monoclonal antibodies (MoAbs). (eur.nl)
  • Naturally occurring IgM antibodies, as well as induced antibodies, can recognize lipopolysaccharide (LPS) epitopes and feed into the NF-κB activation and differentiation cascade with one result being production of immunoglobulin (Ig) by B lymphocytes. (asmscience.org)
  • Among the proteins forming the class of the immunoglobulins, most of them are antibodies and accordingly present an antigen binding site or several antigen binding sites. (justia.com)
  • Anti-idiotype antibodies (anti-Id Abs) are antibodies to idiotopes that are located in the variable region, including the antigen binding site, of another antibody. (frontiersin.org)
  • This book "Anti-idiotype antibodies in cancer treatment" resumes the latest findings in the field. (frontiersin.org)
  • Anti-idiotype antibodies in cancer treatment: the pharmaceutical industry perspective. (frontiersin.org)
  • All the current knowledge of idiotypes of the various antibodies is incorporated in this volume. (elsevier.com)
  • Two mouse monoclonal antibodies to chicken immunoglobulin VH-associated idiotypes (Id), CId-1 and CId-2, were used as probes for Id determinants on mouse T cells. (rupress.org)
  • Complete amino acid sequence of heavy chain variable regions derived from two monoclonal anti-p-azophenylarsonate antibodies of BALB/c mice expressing the major cross-reactive idiotype of the A/J strain. (ac.be)
  • Idiotypic analysis of polyclonal and monoclonal anti-p-azophenylarsonate antibodies of BALB/c mice expressing the major cross-reactive idiotype of the A/J strain. (ac.be)
  • Coutinho and his colleagues, on the other hand, were studying 'natural immunoglobulins' as they emerge as components of newborn mice, and not as 'antibodies' formed in response to specific antigens. (scielo.br)
  • The antibodies against any single determinant may be of several immunoglobulin classes and idiotypes. (elsevier.com)
  • Clinical trials using murine monoclonal anti-idiotype antibodies as a targeting approach have shown some success. (elsevier.com)
  • The theoretic advantages of this approach over the previous use of anti-idiotype antibodies are addressed. (elsevier.com)
  • Previously we reported the use of anti-idiotype antibodies in the therapy of patients with B-cell malignancies. (semanticscholar.org)
  • Because synergy was demonstrated with the addition of alpha interferon to anti-idiotype antibodies in a murine lymphoma model, we performed a clinical trial combining these two agents. (semanticscholar.org)
  • Here we provide an update of the original trial of anti- idiotype antibodies alone and report the outcome of the new combination trial. (semanticscholar.org)
  • idiotypes are antibodies's varible regions (their own antigen recognition site), which are being attacked by other antibodies. (biology-online.org)
  • I did not look in the textbook for this one because I constantly use antibodies for blotting experiments, and idiotype was just something that came to me. (biology-online.org)
  • Immunoglobulins are glycoprotein molecules that function as antibodies and are produced by plasma cells in response to an immunogen. (milkconceptstore.com)
  • Immunoglobulins:Structure and Function • Definition: Glycoprotein molecules that are produced by plasma cells in response to an immunogen and which function as antibodies Immune serum Ag adsorbed serum О± 1 О± 2 ОІ Оі +-albumin globulins Mobility Amount of protein Antibodies fall into five main isotypes, which can be divided further, that have all different effector functions. (milkconceptstore.com)
  • Ultra-structure and Chemical Nature and Functions of Immunoglobulin (Ig) or Antibodies (Ab) Heavy Chains and Light Chains. (milkconceptstore.com)
  • Organization and Arrangement of Heavy and Light Chains in Immunoglobulins - Antibodies. (milkconceptstore.com)
  • idiotypeâ€"anti-idiotype network - a regulatory mechanism mounted in response to the unique or idiotypic determinants of lymphocytes or antibodies upon antigenic stimulation. (enacademic.com)
  • She began her work investigating the cell surface antigens of B lymphocytes and learned that anti-idiotype antibodies directed against the B cell receptor could lead to B cell death in leukemic and lymphomatous cells. (clldigitalarchive.org)
  • Some effects on leukaemic B lymphocytes of antibodies to defined regions of their surface immunoglobulin. (clldigitalarchive.org)
  • The idiotype appears to represent the antigenicity of the antigen-binding site of the antibody and to be genetically codetermined with it. (uams.edu)
  • Pretreatment with cyclosporine and anti-interleukin 2 receptor antibody abrogates the anti-idiotype response in rat recipients of cardiac allografts. (pnas.org)
  • Serological term--antigenicdeterminant unique to a given immunoglobulin.Used to describe the complementaritydetermining regions unique to a given antibody. (slideshare.net)
  • Antibody idiotype is determined by: Gene rearrangement Junctional diversity P-nucleotides (palindromic nucleotides at sites of single-strand breaks) N-nucleotides Somatic hypermutations The word idiotype comes from two Greek roots, idio meaning 'private, distinctive, peculiar' and typos meaning 'mark. (wikipedia.org)
  • The term "idiotype" is sometimes used to describe the collection of multiple idiotopes, and therefore overall antigen binding capacity, possessed by an antibody. (wikipedia.org)
  • He defined idiotype as the set of epitopes on the V region of an antibody molecule, where epitope means an antigenic determinant. (wikipedia.org)
  • Immunoglobulin M , or IgM for short, is a basic antibody that is present on B cells . (bionity.com)
  • It is also the first immunoglobulin expressed in the fetus (around 20 weeks) and also phylogenetically the earliest antibody to develop. (bionity.com)
  • The 16/6 idiotype (Id) antibody, which was the focus of a recent study by Kivity et al. (biomedcentral.com)
  • Dammacco F, Iodice G, Campobasso N: Treatment of adult patients with idiopathic thrombocytopenic purpura with intravenous immunoglobulin: Effects on circulating T cell subsets and PWM-induced antibody synthesis in vitro. (springer.com)
  • The 16/6-idiotype (16/6-Id) of the human anti-DNA antibody was found to induce experimental lupus in naïve mice, manifested by production of autoantibodies, leukopenia and elevated inflammatory markers, as well as kidney and brain involvement. (biomedcentral.com)
  • Human recombinant IL-2 has been linked to the anti-GD2 human-mouse chimeric or humanized forms of the 14.18 antibody (ch14.18 or hu14.18) at the carboxy terminus of the immunoglobulin heavy chain. (asm.org)
  • A determinant that confers on an immunoglobulin molecule an antigenic individuality that is analogous to the individuality of the molecule's antibody activity. (dictionary.com)
  • Study of idiotopic suppression induced by anti-cross-reactive idiotype monoclonal antibody in the anti-p-azophenylarsonate antibody response. (ac.be)
  • The primary objective of this phase 1 study was to evaluate the safety and tolerability of the anti-glucose regulated protein 78 monoclonal immunoglobulin M antibody PAT-SM6 in subjects with relapsed or refractory multiple myeloma. (haematologica.org)
  • The aim of this research was to prepare rabbit Immunoglobulin G as anti-idiotype antibody (Ab2) ofAvian Influenza Virus (AIV) H5N1. (unud.ac.id)
  • Polyvalent immunoglobulin (Ig) G form idiotypic/anti-idiotypic antibody pairs in serum and IVIg preparations. (biomedcentral.com)
  • The immunoglobulins derive their name from the finding that they migrate in the region of globulins when antibody-containing serum is placed in an electrical field. (milkconceptstore.com)
  • anti-idiotype antibody - An antibody directed against the antigen specific part of the sequence of an antibody or T cell receptor. (enacademic.com)
  • anti-idiotype - an·ti id·io·type (an″te idґe o tīp) an antibody directed against an idiotypic determinant of another antibody. (enacademic.com)
  • Antibody - Immunoglobulin redirects here. (enacademic.com)
  • An antibody, also known as an immunoglobulin, is a large Y-shaped protein used by the immune system to identify and neutralize foreign objects such as bacteria and viruses. (absoluteastronomy.com)
  • In immunology, an idiotype is a shared characteristic between a group of immunoglobulin or T-cell receptor (TCR) molecules based upon the antigen binding specificity and therefore structure of their variable region. (wikipedia.org)
  • The word "idiotype" became influential in immunology when Niels Jerne formulated his immune network theory. (wikipedia.org)
  • The idiotype of the immunoglobulin on a given B cell malignancy (Id) can serve as a clonal marker, and a previous pilot study in lymphoma patients has demonstrated that autologous Id protein can be formulated into an immunogenic, tumor specific antigen by conjugation to a carrier protein (KLH) and administration with an emulsion-based adjuvant. (clinicaltrials.gov)
  • The objectives of this study are: 1) to evaluate feasibility and toxicity of new vaccine formulations, and 2) to evaluate cellular and humoral immune responses against the unique idiotype of the patient's lymphoma. (clinicaltrials.gov)
  • The objectives of this study are: 1) to evaluate cellular and humoral immune responses against the unique idiotype of the patient's lymphoma and 2) to evaluate the ability of the Id vaccine to clear the bone marrow of malignant cells detectable by pathologic examination or molecular examination (polymerase chain reaction amplification of the rearranged bcl-2 oncogene). (knowcancer.com)
  • These results suggest that idiotype vaccines may be able to induce a clinically significant immune response against lymphoma. (clinicaltrials.gov)
  • In accordance with this, PCNSLs show rearranged immunoglobulin genes, ongoing somatic hypermutation, and persistent B cell lymphoma 6 (BCL6) activity but lack terminal B-cell differentiation resulting in a fixed IgM/IgD phenotype ( 5 - 8 ). (frontiersin.org)
  • We report here that T cell lines generated from lymphoma patients actively immunized with idiotype protein specifically recognized multiple, unique immunodominant epitopes in autologous tumor idiotype. (jci.org)
  • Idiotype Vaccine Following EPOCH-Rituximab Treatment in Untreated Mantle Cell Lymphoma. (lymphomation.org)
  • When based on hybridoma production techniques, idiotypic vaccination for relapsed B-cell malignancies was consistently feasible only in follicular lymphoma patients, whereas the main cause of failure in other settings was the short survival of idiotype-producing hybridomas. (lymphomation.org)
  • Immunogenicity of a plasmid DNA vaccine encoding chimeric idiotype in patients with B-cell lymphoma. (lymphomation.org)
  • Anti-idiotype vaccines for human follicular lymphoma. (lymphomation.org)
  • Kwak LW, Campbell MJ, Czerwinski DK, Hart S, Miller RA, Levy R Induction of immune responses in patients with B-cell lymphoma against the surface-immunoglobulin idiotype expressed by their tumors. (ucsf.edu)
  • Humoral immune response and immunoglobulin G Fc receptor genotype are associated with better clinical outcome following idiotype vaccination in follicular lymphoma patients regardless of their response to induction chemotherapy. (semanticscholar.org)
  • B-cell lymphoma often has tumor-specific surface immunoglobulins called idiotypes. (elsevier.com)
  • I describe a novel concept of using idiotype-specific peptides as an alternative targeting approach for the treatment of B-cell lymphoma. (elsevier.com)
  • In brief, octapeptides that bind to the surface idiotype of the B-cell lymphoma are isolated from a large synthetic peptide library (10 6 to 10 7 peptides). (elsevier.com)
  • Nevertheless, the molecular characterization of different lymphoid tumor histotypes revealed a set of stereotyped immunoglobulins among distinct B-cell lymphoma types. (unipd.it)
  • Generation of CD8+ T cell-mediated immunity against idiotype-negative lymphoma escapees. (semanticscholar.org)
  • A number of studies have shown that malignant B cells can process and present antigen to T cells in vitro , including the presentation of epitopes derived from their own unique immunoglobulin idiotype to CD4 + and CD8 + T cells ( 5 - 7 ). (aacrjournals.org)
  • While Ab's specific for tumor idiotype have been well described in patients with B cell malignancies, the precise antigenic epitopes in human idiotype recognized by autologous T cells remain largely unknown. (jci.org)
  • Association of idiotype-specific T cell responses with previously documented molecular remissions in idiotype-vaccinated patients suggests that the newly identified T cell epitopes may be clinically relevant. (jci.org)
  • The structure of immunoglobulins perfectly fits their transducer function: the bipolar molecules have variable regions for recognition of diversity of antigenic epitopes and have a few different constant regions which mediate effector functions. (milkconceptstore.com)
  • The variable region of antigen receptors of T cells (TCRs) and B cells (immunoglobulins) contain complementarity-determining regions (CDRs) with unique amino acid sequences. (wikipedia.org)
  • On theoretical and experimental grounds, it has been proposed that the idiotypes of immunoglobulins and of T cell receptors are composed of multiple paratopes, as opposed to a single paratope and several idiotopes. (elsevier.com)
  • Three to six months after completion of chemotherapy, patients who have achieved complete clinical remission or minimal disease status receive a series of 5 injections (given 1-2 months apart) of a vaccine consisting of 0.5 mg autologous tumor-derived immunoglobulin (Id) conjugated to KLH. (clinicaltrials.gov)
  • This cell then secretes the unique idiotype, or immunoglobulin antigen characteristic of the individual tumor, which is purified for use as the vaccine. (wikipedia.org)
  • To assess the clinical benefit in patients with plateau phase multiple myeloma treated with interferon-gamma vs aldesleukin in combination with idiotype-pulsed autologous dendritic cell vaccine APC8020. (knowcancer.com)
  • To perform detailed analyses of lymphocyte phenotypes and T-cell repertoires before and after idiotype-pulsed autologous dendritic cell vaccine APC8020. (knowcancer.com)
  • During culture, supernatant (containing idiotype) is collected until sufficient amounts have been produced to yield adequate dosage of vaccine. (wikipedia.org)
  • [4] Among the 75 patients receiving BiovaxID in the study, 35 received BiovaxID manufactured with an IgM isotype and 40 received BiovaxID manufactured with an IgG isotype with each treatment vaccine produced to correspond with the patient's tumor immunoglobulin isotype. (wikipedia.org)
  • The complete set of idiotopes contained within the variable region of a particular immunoglobulin molecule and making up the molecule's antigen binding site. (yourdictionary.com)
  • A set of one or more antigenic determinants specific to the variable region of an immunoglobulin molecule. (biology-online.org)
  • Antigenic determinants used to describe parts of immunoglobulins: isotypes and idiotypes.2. (slideshare.net)
  • Hädge D, Ambrosius HMol Immunol 1983 Jun 20:597-606Abstract Studies on comparison of antigenic relationships between the immunoglobulin of chicken bi. (gallusimmunotech.com)
  • 16. A method for enhancement of the effect of administration of a vaccinating antigen, an antigenic peptide sequence, or an anti-idiotype immune globulin, in a human or animal body which comprises administering as an adjuvant an effective amount for said enhancement of an immunotherapeutic preparation according to claim 5. (google.es)
  • idiotype - The antigenic specificities defined by the unique sequences (idiotopes) of the antigen combining site. (enacademic.com)
  • Here, we re-examine some of the arguments that underpin this proposed role of joint T cells, in light of recent results from transgenic mice in which a self-reactive T-cell receptor provokes disease, but from outside the joint and indirectly via B lymphocytes and immunoglobulins. (biomedcentral.com)
  • Natural immunoglobulins - In the late 80's, Coutinho and his colleagues produced many dozens of monoclonal immunologlobulins from the spleens of normal newborn Balb/c mice and analyzed the reactivities of these immunoglobulins. (scielo.br)
  • To make a long story short, Coutinho and his colleagues found that the natural immunoglobulins produced by newborn mice react, with variable binding affinities, to virtually everything, although each immunoglobulin had a well defined profile of reactivity. (scielo.br)
  • 1992). Multispecific immunoglobulins are no longer present in the serum of adult mice (Holmberg et al. (scielo.br)
  • mAb-1E10 [ 8 ] is an IgG1 anti-idiotype (Ab2) mAb obtained by immunizing Balb/c mice with mAb-P3 (Ab1) [ 9 ] coupled to keyhole limpet hemocyanin (KLH) in the presence of Freund's adjuvant. (biomedcentral.com)
  • The use of anti-idiotype (anti-id) vaccines for immunotherapy of human cancers is attractive, as immunization with true anti-id reagents (Ab2 beta) has been shown to induce both cellular and humoral immunity, frequently when the original antigen does not, or when a state of anergy to the self-expressed tumor-associated antigen exists. (nih.gov)
  • Targeted DNA vaccines for enhanced induction of idiotype-specific B and T cells. (frontiersin.org)
  • The therapeutic part focusses on harnessing anti-idiotypes for treating autoimmunological disorders, and on the employment of idiotypes for vaccines in cancer and infectious diseases, as well as explaining the manipulation of the idiotypic network in autoimmunity and cancer idiotypes and vaccines. (elsevier.com)
  • Therapeutic vaccines targeting B-NHL idiotype (Id) represent a promising approach to maintain the complete response induced by standard treatments. (unipd.it)
  • The idiotypic determinants have been precisely located to the IMMUNOGLOBULIN VARIABLE REGION of both immunoglobin polypeptide chains. (uams.edu)
  • Thus, idiotype describes the distinctive sequence and region that makes any immunoglobulin/TCR unique from others of the same type which is its variable region. (wikipedia.org)
  • Idiotypic vaccination has already proved capable (in responding patients) of: biological efficacy, that is the capacity of inducing an idiotype- and tumor-specific immune response (Kwak LW et al. (clinicaltrials.gov)
  • anti-idiotype - anti idiotypic /an tee id ee euh tip ik, an tuy /, adj. (enacademic.com)
  • Idiotypic determinants on the surface immunoglobulin of neoplastic lymphocytes: a therapeutic target. (clldigitalarchive.org)
  • The clonotypic surface Ig receptor expressed by malignant B cells, idiotype, is a tumor-specific antigen and an attractive target for active immunotherapy. (jci.org)
  • In follicular center cell lymphomas, another incurable disease, recent evidence suggests that molecular complete remissions may be achieved following idiotype vaccination in patients who have achieved minimal residual disease with combination chemotherapy. (clinicaltrials.gov)
  • Feasibility of idiotype vaccination was statistically compared among five different B-cell malignancies in first relapse. (lymphomation.org)
  • Anti-idiotype vaccination represents an innovative approach to target tumor-associated antigen-expressing cells. (biomedcentral.com)
  • Analysis of genetic relatedness between the two cancers considered concordance for immunoglobulin gene rearrangements, for immunoglobulin isotypes and idiotypes, and for cytogenetic abnormalities. (annals.org)
  • To compare T-cell immunity using two different methods of isolating the idiotype protein. (clinicaltrials.gov)
  • According to the theory of in vitro radioassay, using TSHAb as binder and 125I- staphylococcal protein A (125I-SPA) as tracer agent, a new method for detecting the abnormal immunoglobulin (aIgG) in the sera of patients with Graves disease (GD) was reported. (bvsalud.org)
  • This approach is used because there is a unique protein called an "idiotype" expressed exclusively on the cancerous B-cells. (wikipedia.org)
  • Staphlococcal induced human platelet injury mediated by protein A and immunoglobulin G Fc fragment receptor. (springer.com)
  • The measurement of intact ch14.18-IL-2 fusion protein is not confounded by free IL-2 or free ch14.18 when 100 ng or less of total immunoglobulin per ml is used during the assay procedure. (asm.org)
  • For example, spectrophotometric assays are confounded by other proteins in the serum (Bradford, Lowry, or bicinchoninic acid protein assay systems), and enzyme-linked immunosorbent assays (ELISAs) quantitating immunoglobulin G (IgG) are unable to distinguish the intact fusion protein from the parent immunoglobulin. (asm.org)
  • A new form of immunoblot (Panama blot), by which the reaction of natural immunoglobulins with complex protein mixtures may be analyzed by a special software and multivariate statistics, has been recently used to characterize human autoimmune diseases. (scielo.br)
  • Id-secreting clones are identified by a bioinformatics approach which confirms a match of each vaccine's unique idiotype sequence to the tumor's after which they are cultured (expanded) in a personal-scale, disposable hollow-fiber AutovaxID™ bioreactor system. (wikipedia.org)
  • A number of clinical trials have examined the efficacy of epitope-modified DCs, most often by "pulsing" DCs ex vivo with synthetic peptides based on antigens (5 , 6 , 7 , 8 , 9 , 10) , mutant oncogenes (11) , or immunoglobulin idiotypes (12) expressed by tumors. (aacrjournals.org)
  • Cellular expression of rheumatoid factor cross-reactive idiotype in patients with seropositive and seronegative rheumatoid arthritis. (biomedsearch.com)
  • A study has been made of the presence of the major rheumatoid Factor cross-reacting idiotype (RCRI) in the serum of patients with seropositive and seronegative rheumatoid arthritis as well as in the cytoplasm of plasmacells induced by cultivation of peripheral blood leucocytes (PBL) from the same RA patients and from normal humans. (biomedsearch.com)
  • More representative data on the B-cell receptor repertoire can be obtained by the clonotype analysis of anti-idiotype-treated animals (1,2) but, in this case, different immunization protocols can also produce misleading results. (springer.com)
  • Using in vitro immunization, we have reconstructed three consecutive steps of an idiotype network to show that the nucleocapsid of Semliki Forest virus contains a specific 'receptor' for the cytoplasmic tail of the E2 spike glycoprotein. (ox.ac.uk)
  • Such immunoglobulins can be used for several purposes, especially for diagnosis or therapeutical purposes including protection against pathological agents or regulation of the expression or activity of proteins. (justia.com)
  • The best developed version of immune network theory is called the symmetrical network theory, in which the distinction between idiotype and paratope plays no role. (wikipedia.org)
  • An alternative way to obtain a quantitative description of the repertoire of both mature and precursor B cells is to polyclonally expand the B-cell pool and to study the frequency of B cells which are precursors for clones secreting immunoglobulin with a given paratope or idiotype. (springer.com)
  • A paratope cannot therefore be regarded as restricted to or unique on an idiotype. (elsevier.com)
  • Those rabbitantibodies recognize isotypic determinants,and will react with immunoglobulins in theserum of virtually all humans, since allhumans express γ1 heavy chains and κ lightchains. (slideshare.net)
  • The clonotypic idiotype of malignant B cells was the first tumor-specific antigen identified as capable of eliciting a T cell-mediated immune response ( 1 , 2 ). (aacrjournals.org)
  • In B cell malignancies, the clonotypic surface Ig expressed by malignant B cells, idiotype (Id), is a tumor-specific antigen ( 4 - 6 ) and, therefore, provides a unique opportunity to target the antitumor immune responses. (jci.org)
  • Intradermal administration of uncoupled recombinant idiotype with appropriate adjuvants may overcome profound clinical immunosuppression and induce specific immune responses. (lymphomation.org)
  • Idiotype (Id) 16/6 marks a variable (V) region structure that occurs frequently in the human immunoglobulin repertoire. (rupress.org)
  • Human immunoglobulin A (IgA) is the second most common human immunoglobulin in serum. (milkconceptstore.com)
  • APC8020 is generated by loading DC with immunoglobulin idiotype prepared from the patient's serum. (knowcancer.com)
  • Up to now the structure proposed for immunoglobulins consists of a four-chain model referring to the presence of two identical light polypeptide chains (light chains) and two identical heavy polypeptide chains (heavy chains) linked together by disulfide bonds to form a y- or T-shaped macromolecules. (justia.com)
  • The inventors have surprisingly established that different molecules can be isolated from animals which naturally produce them, which molecules have functional properties of immunoglobulins these functions being in some cases related to structural elements which are distinct from those involved in the function of four-chain immunoglobulins due for instance to the absence of light chains. (justia.com)
  • There is provided an isolated immunoglobulin comprising two heavy polypeptide chains sufficient for the formation of a complete antigen binding site or several antigen binding sites, wherein the immunoglobulin is further devoid of light polypeptide chains. (freepatentsonline.com)
  • The invention does not start from the idea that the different fragments (light and heavy chains) and the different domains of these fragments of four-chain model immunoglobulin can be modified to define new or improved antigen binding sites or a four-chain model immunoglobulin. (justia.com)
  • Immunoglobulin Idiotypes and Their Expression: ICN-UCLA Symposia on Molecular and Cellular Biology , Volume XX (C. A. Janeway, Jr., E. E. Sercarz, and H. Wigzell, eds. (springer.com)
  • The invention relates to new isolated immunoglobulins which are devoid of light polypeptide chains. (justia.com)
  • These immunoglobulins do not consist in the degradation products of immunoglobulins composed of both heavy polypeptide and light polypeptide chains but to the contrary, the invention defines a new member of the family of the immunoglobulins, especially a new type of molecules capable of being involved in the immune recognition. (justia.com)
  • Intravenous immunoglobulin (IVIG) products are prepared from purified plasma immunoglobulins from large numbers of healthy donors. (biomedcentral.com)
  • Intravenous immunoglobulin (IVIG) products are being investigated as potential agents for treatment or prevention of AD. (biomedcentral.com)
  • Intravenous immunoglobulin (IVIg) is an effective treatment in chronic inflammatory demyelinating polyneuropathy (CIDP). (biomedcentral.com)
  • 1987). This multireactivity forms a network of immunoglobulins early in the operation of the immune system and interference with their formation results in distortions of the adult B cell repertoire (Vakil et al. (scielo.br)