Immunoglobulin Fab Fragments
Univalent antigen-binding fragments composed of one entire IMMUNOGLOBULIN LIGHT CHAIN and the amino terminal end of one of the IMMUNOGLOBULIN HEAVY CHAINS from the hinge region, linked to each other by disulfide bonds. Fab contains the IMMUNOGLOBULIN VARIABLE REGIONS, which are part of the antigen-binding site, and the first IMMUNOGLOBULIN CONSTANT REGIONS. This fragment can be obtained by digestion of immunoglobulins with the proteolytic enzyme PAPAIN.
Multi-subunit proteins which function in IMMUNITY. They are produced by B LYMPHOCYTES from the IMMUNOGLOBULIN GENES. They are comprised of two heavy (IMMUNOGLOBULIN HEAVY CHAINS) and two light chains (IMMUNOGLOBULIN LIGHT CHAINS) with additional ancillary polypeptide chains depending on their isoforms. The variety of isoforms include monomeric or polymeric forms, and transmembrane forms (B-CELL ANTIGEN RECEPTORS) or secreted forms (ANTIBODIES). They are divided by the amino acid sequence of their heavy chains into five classes (IMMUNOGLOBULIN A; IMMUNOGLOBULIN D; IMMUNOGLOBULIN E; IMMUNOGLOBULIN G; IMMUNOGLOBULIN M) and various subclasses.
Immunoglobulin Heavy Chains
Immunoglobulin Light Chains
Genes encoding the different subunits of the IMMUNOGLOBULINS, for example the IMMUNOGLOBULIN LIGHT CHAIN GENES and the IMMUNOGLOBULIN HEAVY CHAIN GENES. The heavy and light immunoglobulin genes are present as gene segments in the germline cells. The completed genes are created when the segments are shuffled and assembled (B-LYMPHOCYTE GENE REARRANGEMENT) during B-LYMPHOCYTE maturation. The gene segments of the human light and heavy chain germline genes are symbolized V (variable), J (joining) and C (constant). The heavy chain germline genes have an additional segment D (diversity).
Immunoglobulin preparations used in intravenous infusion, containing primarily IMMUNOGLOBULIN G. They are used to treat a variety of diseases associated with decreased or abnormal immunoglobulin levels including pediatric AIDS; primary HYPERGAMMAGLOBULINEMIA; SCID; CYTOMEGALOVIRUS infections in transplant recipients, LYMPHOCYTIC LEUKEMIA, CHRONIC; Kawasaki syndrome, infection in neonates, and IDIOPATHIC THROMBOCYTOPENIC PURPURA.
Immunoglobulin Fc Fragments
Crystallizable fragments composed of the carboxy-terminal halves of both IMMUNOGLOBULIN HEAVY CHAINS linked to each other by disulfide bonds. Fc fragments contain the carboxy-terminal parts of the heavy chain constant regions that are responsible for the effector functions of an immunoglobulin (COMPLEMENT fixation, binding to the cell membrane via FC RECEPTORS, and placental transport). This fragment can be obtained by digestion of immunoglobulins with the proteolytic enzyme PAPAIN.
Immunoglobulin Variable Region
That region of the immunoglobulin molecule that varies in its amino acid sequence and composition, and comprises the binding site for a specific antigen. It is located at the N-terminus of the Fab fragment of the immunoglobulin. It includes hypervariable regions (COMPLEMENTARITY DETERMINING REGIONS) and framework regions.
Binding Sites, Antibody
Molecular Sequence Data
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
Amino Acid Sequence
Immunoglobulin A, Secretory
Immunoglobulin Constant Regions
The domains of the immunoglobulin molecules that are invariable in their amino acid sequence within any class or subclass of immunoglobulin. They confer biological as well as structural functions to immunoglobulins. One each on both the light chains and the heavy chains comprises the C-terminus half of the IMMUNOGLOBULIN FAB FRAGMENT and two or three of them make up the rest of the heavy chains (all of the IMMUNOGLOBULIN FC FRAGMENT)
Enzyme-Linked Immunosorbent Assay
An immunoassay utilizing an antibody labeled with an enzyme marker such as horseradish peroxidase. While either the enzyme or the antibody is bound to an immunosorbent substrate, they both retain their biologic activity; the change in enzyme activity as a result of the enzyme-antibody-antigen reaction is proportional to the concentration of the antigen and can be measured spectrophotometrically or with the naked eye. Many variations of the method have been developed.
A measure of the binding strength between antibody and a simple hapten or antigen determinant. It depends on the closeness of stereochemical fit between antibody combining sites and antigen determinants, on the size of the area of contact between them, and on the distribution of charged and hydrophobic groups. It includes the concept of "avidity," which refers to the strength of the antigen-antibody bond after formation of reversible complexes.
Polymorphism, Restriction Fragment Length
Heavy chains of IMMUNOGLOBULIN G having a molecular weight of approximately 51 kDa. They contain about 450 amino acid residues arranged in four domains and an oligosaccharide component covalently bound to the Fc fragment constant region. The gamma heavy chain subclasses (for example, gamma 1, gamma 2a, and gamma 2b) of the IMMUNOGLOBULIN G isotype subclasses (IgG1, IgG2A, and IgG2B) resemble each other more closely than the heavy chains of the other IMMUNOGLOBULIN ISOTYPES.
Immunoglobulin Class Switching
Gene rearrangement of the B-lymphocyte which results in a substitution in the type of heavy-chain constant region that is expressed. This allows the effector response to change while the antigen binding specificity (variable region) remains the same. The majority of class switching occurs by a DNA recombination event but it also can take place at the level of RNA processing.
A 15 kD "joining" peptide that forms one of the linkages between monomers of IMMUNOGLOBULIN A or IMMUNOGLOBULIN M in the formation of polymeric immunoglobulins. There is one J chain per one IgA dimer or one IgM pentamer. It is also involved in binding the polymeric immunoglobulins to POLYMERIC IMMUNOGLOBULIN RECEPTOR which is necessary for their transcytosis to the lumen. It is distinguished from the IMMUNOGLOBULIN JOINING REGION which is part of the IMMUNOGLOBULIN VARIABLE REGION of the immunoglobulin light and heavy chains.
Unique genetically-controlled determinants present on ANTIBODIES whose specificity is limited to a single group of proteins (e.g., another antibody molecule or an individual myeloma protein). The idiotype appears to represent the antigenicity of the antigen-binding site of the antibody and to be genetically codetermined with it. The idiotypic determinants have been precisely located to the IMMUNOGLOBULIN VARIABLE REGION of both immunoglobin polypeptide chains.
The measurement of infection-blocking titer of ANTISERA by testing a series of dilutions for a given virus-antiserum interaction end-point, which is generally the dilution at which tissue cultures inoculated with the serum-virus mixtures demonstrate cytopathology (CPE) or the dilution at which 50% of test animals injected with serum-virus mixtures show infectivity (ID50) or die (LD50).
A species of gram-negative, facultatively anaerobic, rod-shaped bacteria (GRAM-NEGATIVE FACULTATIVELY ANAEROBIC RODS) commonly found in the lower part of the intestine of warm-blooded animals. It is usually nonpathogenic, but some strains are known to produce DIARRHEA and pyogenic infections. Pathogenic strains (virotypes) are classified by their specific pathogenic mechanisms such as toxins (ENTEROTOXIGENIC ESCHERICHIA COLI), etc.
Serum globulins that migrate to the gamma region (most positively charged) upon ELECTROPHORESIS. At one time, gamma-globulins came to be used as a synonym for immunoglobulins since most immunoglobulins are gamma globulins and conversely most gamma globulins are immunoglobulins. But since some immunoglobulins exhibit an alpha or beta electrophoretic mobility, that usage is in decline.
Receptors, Polymeric Immunoglobulin
Specialized Fc receptors (RECEPTORS, FC) for polymeric immunoglobulins, which mediate transcytosis of polymeric IMMUNOGLOBULIN A and IMMUNOGLOBULIN M into external secretions. They are found on the surfaces of epithelial cells and hepatocytes. After binding to IMMUNOGLOBULIN A, the receptor-ligand complex undergoes endocytosis, transport by vesicle, and secretion into the lumen by exocytosis. Before release, the part of the receptor (SECRETORY COMPONENT) that is bound to IMMUNOGLOBULIN A is proteolytically cleaved from its transmembrane tail. (From Rosen et al., The Dictionary of Immunology, 1989)
The characteristic 3-dimensional shape of a protein, including the secondary, supersecondary (motifs), tertiary (domains) and quaternary structure of the peptide chain. PROTEIN STRUCTURE, QUATERNARY describes the conformation assumed by multimeric proteins (aggregates of more than one polypeptide chain).
Immunoglobulin Joining Region
A segment of the immunoglobulin heavy chains, encoded by the IMMUNOGLOBULIN HEAVY CHAIN GENES in the J segment where, during the maturation of B-LYMPHOCYTES; the gene segment for the variable region upstream is joined to a constant region gene segment downstream. The exact position of joining of the two gene segments is variable and contributes to ANTIBODY DIVERSITY. It is distinguished from the IMMUNOGLOBULIN J CHAINS; a separate polypeptide that serves as a linkage piece in polymeric IGA or IGM.
Electrophoresis, Polyacrylamide Gel
A technique that combines protein electrophoresis and double immunodiffusion. In this procedure proteins are first separated by gel electrophoresis (usually agarose), then made visible by immunodiffusion of specific antibodies. A distinct elliptical precipitin arc results for each protein detectable by the antisera.
A cardiotonic glycoside obtained mainly from Digitalis lanata; it consists of three sugars and the aglycone DIGOXIGENIN. Digoxin has positive inotropic and negative chronotropic activity. It is used to control ventricular rate in ATRIAL FIBRILLATION and in the management of congestive heart failure with atrial fibrillation. Its use in congestive heart failure and sinus rhythm is less certain. The margin between toxic and therapeutic doses is small. (From Martindale, The Extra Pharmacopoeia, 30th ed, p666)
A deoxyribonucleotide polymer that is the primary genetic material of all cells. Eukaryotic and prokaryotic organisms normally contain DNA in a double-stranded state, yet several important biological processes transiently involve single-stranded regions. DNA, which consists of a polysugar-phosphate backbone possessing projections of purines (adenine and guanine) and pyrimidines (thymine and cytosine), forms a double helix that is held together by hydrogen bonds between these purines and pyrimidines (adenine to thymine and guanine to cytosine).
Receptors, Antigen, B-Cell
Polymerase Chain Reaction
In vitro method for producing large amounts of specific DNA or RNA fragments of defined length and sequence from small amounts of short oligonucleotide flanking sequences (primers). The essential steps include thermal denaturation of the double-stranded target molecules, annealing of the primers to their complementary sequences, and extension of the annealed primers by enzymatic synthesis with DNA polymerase. The reaction is efficient, specific, and extremely sensitive. Uses for the reaction include disease diagnosis, detection of difficult-to-isolate pathogens, mutation analysis, genetic testing, DNA sequencing, and analyzing evolutionary relationships.
Fluorescent Antibody Technique
Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
Staphylococcal Protein A
A protein present in the cell wall of most Staphylococcus aureus strains. The protein selectively binds to the Fc region of human normal and myeloma-derived IMMUNOGLOBULIN G. It elicits antibody activity and may cause hypersensitivity reactions due to histamine release; has also been used as cell surface antigen marker and in the clinical assessment of B lymphocyte function.
Genes, Immunoglobulin Heavy Chain
Somatic Hypermutation, Immunoglobulin
Recombinant Fusion Proteins
Protein Structure, Tertiary
The level of protein structure in which combinations of secondary protein structures (alpha helices, beta sheets, loop regions, and motifs) pack together to form folded shapes called domains. Disulfide bridges between cysteines in two different parts of the polypeptide chain along with other interactions between the chains play a role in the formation and stabilization of tertiary structure. Small proteins usually consist of only one domain but larger proteins may contain a number of domains connected by segments of polypeptide chain which lack regular secondary structure.
White blood cells formed in the body's lymphoid tissue. The nucleus is round or ovoid with coarse, irregularly clumped chromatin while the cytoplasm is typically pale blue with azurophilic (if any) granules. Most lymphocytes can be classified as either T or B (with subpopulations of each), or NATURAL KILLER CELLS.
Immunoglobulin Switch Region
A site located in the INTRONS at the 5' end of each constant region segment of a immunoglobulin heavy-chain gene where recombination (or rearrangement) occur during IMMUNOGLOBULIN CLASS SWITCHING. Ig switch regions are found on genes encoding all five classes (IMMUNOGLOBULIN ISOTYPES) of IMMUNOGLOBULIN HEAVY CHAINS.
Specific molecular sites on the surface of various cells, including B-lymphocytes and macrophages, that combine with IMMUNOGLOBULIN Gs. Three subclasses exist: Fc gamma RI (the CD64 antigen, a low affinity receptor), Fc gamma RII (the CD32 antigen, a high affinity receptor), and Fc gamma RIII (the CD16 antigen, a low affinity receptor).
DNA Restriction Enzymes
Enzymes that are part of the restriction-modification systems. They catalyze the endonucleolytic cleavage of DNA sequences which lack the species-specific methylation pattern in the host cell's DNA. Cleavage yields random or specific double-stranded fragments with terminal 5'-phosphates. The function of restriction enzymes is to destroy any foreign DNA that invades the host cell. Most have been studied in bacterial systems, but a few have been found in eukaryotic organisms. They are also used as tools for the systematic dissection and mapping of chromosomes, in the determination of base sequences of DNAs, and have made it possible to splice and recombine genes from one organism into the genome of another. EC 3.21.1.
Lymphocytes responsible for cell-mediated immunity. Two types have been identified - cytotoxic (T-LYMPHOCYTES, CYTOTOXIC) and helper T-lymphocytes (T-LYMPHOCYTES, HELPER-INDUCER). They are formed when lymphocytes circulate through the THYMUS GLAND and differentiate to thymocytes. When exposed to an antigen, they divide rapidly and produce large numbers of new T cells sensitized to that antigen.
The extracellular moiety of the POLYMERIC IMMUNOGLOBULIN RECEPTOR found alone or complexed with IGA or IGM, in a variety of external secretions (tears, bile, colostrum.) Secretory component is derived by proteolytic cleavage of the receptor during transcytosis. When immunoglobulins IgA and IgM are bound to the receptor, during their transcytosis secretory component becomes covalently attached to them generating SECRETORY IMMUNOGLOBULIN A or secretory IMMUNOGLOBULIN M.
Microscopy using an electron beam, instead of light, to visualize the sample, thereby allowing much greater magnification. The interactions of ELECTRONS with specimens are used to provide information about the fine structure of that specimen. In TRANSMISSION ELECTRON MICROSCOPY the reactions of the electrons that are transmitted through the specimen are imaged. In SCANNING ELECTRON MICROSCOPY an electron beam falls at a non-normal angle on the specimen and the image is derived from the reactions occurring above the plane of the specimen.
Complement System Proteins
Serum glycoproteins participating in the host defense mechanism of COMPLEMENT ACTIVATION that creates the COMPLEMENT MEMBRANE ATTACK COMPLEX. Included are glycoproteins in the various pathways of complement activation (CLASSICAL COMPLEMENT PATHWAY; ALTERNATIVE COMPLEMENT PATHWAY; and LECTIN COMPLEMENT PATHWAY).
A glycoprotein that is central in both the classical and the alternative pathway of COMPLEMENT ACTIVATION. C3 can be cleaved into COMPLEMENT C3A and COMPLEMENT C3B, spontaneously at low level or by C3 CONVERTASE at high level. The smaller fragment C3a is an ANAPHYLATOXIN and mediator of local inflammatory process. The larger fragment C3b binds with C3 convertase to form C5 convertase.
Sequence Analysis, DNA
Members of the class of compounds composed of AMINO ACIDS joined together by peptide bonds between adjacent amino acids into linear, branched or cyclical structures. OLIGOPEPTIDES are composed of approximately 2-12 amino acids. Polypeptides are composed of approximately 13 or more amino acids. PROTEINS are linear polypeptides that are normally synthesized on RIBOSOMES.
Immunoglobulin Gm Allotypes
Nucleic Acid Hybridization
Widely used technique which exploits the ability of complementary sequences in single-stranded DNAs or RNAs to pair with each other to form a double helix. Hybridization can take place between two complimentary DNA sequences, between a single-stranded DNA and a complementary RNA, or between two RNA sequences. The technique is used to detect and isolate specific sequences, measure homology, or define other characteristics of one or both strands. (Kendrew, Encyclopedia of Molecular Biology, 1994, p503)
Deliberate stimulation of the host's immune response. ACTIVE IMMUNIZATION involves administration of ANTIGENS or IMMUNOLOGIC ADJUVANTS. PASSIVE IMMUNIZATION involves administration of IMMUNE SERA or LYMPHOCYTES or their extracts (e.g., transfer factor, immune RNA) or transplantation of immunocompetent cell producing tissue (thymus or bone marrow).
The restriction of a characteristic behavior, anatomical structure or physical system, such as immune response; metabolic response, or gene or gene variant to the members of one species. It refers to that property which differentiates one species from another but it is also used for phylogenetic levels higher or lower than the species.
Sequence Homology, Amino Acid
Combinatorial Chemistry Techniques
Sequence Homology, Nucleic Acid
Gene Rearrangement, B-Lymphocyte, Heavy Chain
Gene Rearrangement, B-Lymphocyte
Plasma glycoprotein clotted by thrombin, composed of a dimer of three non-identical pairs of polypeptide chains (alpha, beta, gamma) held together by disulfide bonds. Fibrinogen clotting is a sol-gel change involving complex molecular arrangements: whereas fibrinogen is cleaved by thrombin to form polypeptides A and B, the proteolytic action of other enzymes yields different fibrinogen degradation products.
Platelet Membrane Glycoproteins
Procedures by which protein structure and function are changed or created in vitro by altering existing or synthesizing new structural genes that direct the synthesis of proteins with sought-after properties. Such procedures may include the design of MOLECULAR MODELS of proteins using COMPUTER GRAPHICS or other molecular modeling techniques; site-specific mutagenesis (MUTAGENESIS, SITE-SPECIFIC) of existing genes; and DIRECTED MOLECULAR EVOLUTION techniques to create new genes.
Dose-Response Relationship, Immunologic
The scattering of x-rays by matter, especially crystals, with accompanying variation in intensity due to interference effects. Analysis of the crystal structure of materials is performed by passing x-rays through them and registering the diffraction image of the rays (CRYSTALLOGRAPHY, X-RAY). (From McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)
Technique using an instrument system for making, processing, and displaying one or more measurements on individual cells obtained from a cell suspension. Cells are usually stained with one or more fluorescent dyes specific to cell components of interest, e.g., DNA, and fluorescence of each cell is measured as it rapidly transverses the excitation beam (laser or mercury arc lamp). Fluorescence provides a quantitative measure of various biochemical and biophysical properties of the cell, as well as a basis for cell sorting. Other measurable optical parameters include light absorption and light scattering, the latter being applicable to the measurement of cell size, shape, density, granularity, and stain uptake.
Differentiation antigens residing on mammalian leukocytes. CD stands for cluster of differentiation, which refers to groups of monoclonal antibodies that show similar reactivity with certain subpopulations of antigens of a particular lineage or differentiation stage. The subpopulations of antigens are also known by the same CD designation.
Cell Adhesion Molecules
Morphologic alteration of small B LYMPHOCYTES or T LYMPHOCYTES in culture into large blast-like cells able to synthesize DNA and RNA and to divide mitotically. It is induced by INTERLEUKINS; MITOGENS such as PHYTOHEMAGGLUTININS, and by specific ANTIGENS. It may also occur in vivo as in GRAFT REJECTION.
Complementarity Determining Regions
Three regions (CDR1; CDR2 and CDR3) of amino acid sequence in the IMMUNOGLOBULIN VARIABLE REGION that are highly divergent. Together the CDRs from the light and heavy immunoglobulin chains form a surface that is complementary to the antigen. These regions are also present in other members of the immunoglobulin superfamily, for example, T-cell receptors (RECEPTORS, ANTIGEN, T-CELL).
Antibodies, often monoclonal, in which the two antigen-binding sites are specific for separate ANTIGENIC DETERMINANTS. They are artificial antibodies produced by chemical crosslinking, fusion of HYBRIDOMA cells, or by molecular genetic techniques. They function as the main mediators of targeted cellular cytotoxicity and have been shown to be efficient in the targeting of drugs, toxins, radiolabeled haptens, and effector cells to diseased tissue, primarily tumors.
Interaction of inflammatory cells and oral microorganisms. III. Modulation of rabbit polymorphonuclear leukocyte hydrolase release response to Actinomyces viscosus and Streptococcus mutans by immunoglobulins and complement. (1/2903)In the absence of antiserum, rabbit polymorphonuclear leukocytes (PMNs) released lysosomal enzymes in response to Actinomyces viscosus (19246) but not to Streptococcus mutans (6715). Antibodies had a marked modulating influence on these reactions. PMN hydrolase release was significantly enhanced to both organisms when specific rabbit antiserum and isolated immunoglobulin G (IgG) were included in the incubations. Immune complex F(ab')2 fragments of IgG directed against S. mutans agglutinated bacteria. Immune complexes consisting of S. mutans and F(ab')2 fragments of IgG directed against this organism were not effective as bacteria-IgG complexes in stimulating PMN release. The intensity of the release response to bacteria-IgG complexes was also diminished when PMNs were preincubated with isolated Fc fragments derived from IgG. Fresh serum as a source of complement components had no demonstrable effect on PMN release either alone or in conjuction with antiserum in these experiments. These data may be relevant to the mechanisms and consequences of the interaction of PMNs and plaque bacteria in the pathogenesis of periodontal disease. (+info)
Labeling of the internal pool of GP IIb-IIIa in platelets by c7E3 Fab fragments (abciximab): flow and endocytic mechanisms contribute to the transport. (2/2903)Abciximab is a new antiplatelet therapeutic in ischemic cardiovascular disease. The drug, chimeric Fab fragments of a murine monoclonal antibody (MoAb) (c7E3), blocks GP IIb-IIIa function. However, its capacity to reach all receptor pools in platelets is unknown. Electron microscopy and immunogold labeling were used to localize abciximab in platelets of patients receiving the drug for up to 24 hours. Studies on frozen-thin sections showed that c7E3 Fab, in addition to the surface pool, also labeled the surface-connected canalicular system (SCCS) and alpha-granules. Analysis of gold particle distribution showed that intraplatelet labeling was not accumulative and in equilibrium with the surface pool. After short-term incubations of platelets with c7E3 Fab in vitro, gold particles were often seen in lines within thin elements of the SCCS, some of which appeared in contact with alpha-granules. Little labeling was associated with Glanzmann's thrombasthenia platelets, confirming that the channels contained bound and not free c7E3 Fab. Endocytosis of abciximab in clathrin-containing vesicles was visualized by double staining and constitutes an alternative mechanism of transport. The remaining free pool of GP IIb-IIIa was evaluated with the MoAb AP-2; flow cytometry showed it to be about 9% on the surface of nonstimulated platelets but 33% on thrombin-activated platelets. The ability of drugs to block all pools of GP IIb-IIIa and then to be associated with secretion-dependent residual aggregation must be considered when evaluating their efficiency in a clinical context. (+info)
Recognition of polynucleotides by antibodies to poly(I), poly(C). (3/2903)The binding of anti poly(I). poly (C) Fab fragments to double or triple stranded polynucletides has been studied by fluorescence. Association constants were deduced from competition experiments. The comparison of the association constants leads to the conclusion that several atoms of the base residues do not interact with the amino acid residues of the binding site of Fab fragment while the hydroxyl groups of furanose rings interact. These results suggest that the Fab fragments do not bind to the major groove of the double stranded polynucleotides. An interaction between the C(2)O group of pyrimidine residues and Fab fragments cannot be excluded. Circular dichroism of poly(I). poly(C) or poly(I). poly(br5C)-Fab fragments complexes are very different from the circular dichroism of free polynucleotides which suggests a deformation of the polynucleotides bound to the Fab fragments. (+info)
Efficient IgG-mediated suppression of primary antibody responses in Fcgamma receptor-deficient mice. (4/2903)IgG antibodies can suppress more than 99% of the antibody response against the antigen to which they bind. This is used clinically to prevent rhesus-negative (Rh-) women from becoming immunized against Rh+ erythrocytes from their fetuses. The suppressive mechanism is poorly understood, but it has been proposed that IgG/erythrocyte complexes bind to the inhibitory Fc receptor for IgG (FcgammaRIIB) on the B cell surface, thereby triggering negative signals that turn off the B cell. We show that IgG induces the same degree of suppression of the response to sheep erythrocytes in animals lacking the known IgG-binding receptors FcgammaRIIB, FcgammaRI + III, FcgammaRI + IIB + III, and FcRn (the neonatal Fc receptor) as in wild-type animals. Reinvestigation of the ability of F(ab')2 fragments to suppress antibody responses demonstrated that they were nearly as efficient as intact IgG. In addition, monoclonal IgE also was shown to be suppressive. These findings suggest that IgG inhibits antibody responses through Fc-independent mechanisms, most likely by masking of antigenic epitopes, thereby preventing B cells from binding and responding to antigen. In agreement with this, we show that T cell priming is not abolished by passively administered IgG. The results have implications for the understanding of in vivo regulation of antibody responses and Rh prophylaxis. (+info)
Head-to-tail dimers and interdomain flexibility revealed by the crystal structure of HIV-1 capsid protein (p24) complexed with a monoclonal antibody Fab. (5/2903)The crystal structure of an intact molecule of HIV-1 capsid protein (p24) in complex with a monoclonal antibody fragment recognizing an epitope on the C-terminal domain has been determined at 3 A resolution. The helical N- and C-terminal domains of p24 are linked by an extended peptide forming a flexibly linked dumb-bell-shaped molecule 75 A in overall length. The p24 construct used is a variant with an N-terminal extension that mimics to some extent the Gag context of p24. We observed a novel head-to-tail dimer of p24 molecules which occurs through the formation of a substantial intermolecular interface between the N- and C-terminal domains. Comparison with previously observed p24 dimers shows that the same residues and secondary structural elements can partake in different interfaces revealing a remarkable stickiness and plasticity of the p24 molecule, properties which, combined with the inter-domain flexibility, are presumably important in the assembly and maturation of viral particles. Previous mutagenesis studies designed to test specific N-N and C-C homodimer interfaces do not discriminate fully against the possibility of the observed N-C interface. (+info)
Flexibility of the major antigenic loop of foot-and-mouth disease virus bound to a Fab fragment of a neutralising antibody: structure and neutralisation. (6/2903)The interaction of foot-and-mouth disease virus (FMDV) serotype C (clone C-S8c1) with a strongly neutralising monoclonal antibody (MAb) 4C4 has been studied by combining data from cryoelectron microscopy and x-ray crystallography. The MAb 4C4 binds to the exposed flexible GH-loop of viral protein 1 (VP1), which appears to retain its flexibility, allowing movement of the bound Fab. This is in striking contrast to MAb SD6, which binds to the same GH-loop of VP1 but exhibits no movement of the bound Fab when observed under identical conditions. However, MAbs 4C4 and SD6 have very similar neutralisation characteristics. The known atomic structure of FMDV C-S8c1 and that of the 4C4 Fab cocrystallised with a synthetic peptide corresponding to the GH-loop of VP1 were fitted to the cryoelectron microscope density map. The best fit of the 4C4 Fab is compatible only with monovalent binding of the MAb in agreement with the neutralisation data on 4C4 MAbs, Fab2s, and Fabs. The position of the bound GH-loop is related to other known positions of this loop by a hinge rotation about the base of the loop. The 4C4 Fab appears to interact almost exclusively with the G-H loop of VP1, making no other contacts with the viral capsid. (+info)
Analysis of the interaction of monoclonal antibodies with surface IgM on neoplastic B-cells. (7/2903)In vitro studies identified three Burkitts lymphoma cell lines, Ramos, MUTU-I and Daudi, that were growth inhibited by anti-IgM antibody. However, only Ramos and MUTU-I were sensitive to monoclonal antibodies (mAb) recognizing the Fc region of surface IgM (anti-Fc mu). Experiments using anti-Fc mu mAb (single or non-crossblocking pairs), polyclonal anti-mu Ab, and hyper-crosslinking with a secondary layer of Ab, showed that growth inhibition of B-cell lines was highly dependent on the extent of IgM crosslinking. This was confirmed by using Fab', F(ab')2 and F(ab')3 derivatives from anti-Fc mu mAb, where increasing valency caused corresponding increases in growth arrest and apoptosis, presumably as a result of more efficient BCR-crosslinking on the cell surface. The ability of a single mAb to induce growth arrest was highly dependent on epitope specificity, with mAb specific for the Fc region (C mu2-C mu4 domains) being much more effective than those recognizing the Fab region (anti-L chain, anti-Id and anti-Fd mu, or C mu1). Only when hyper-crosslinked with polyclonal anti-mouse IgG did the latter result in appreciable growth inhibition. Binding studies showed that these differences in function were not related to differences in the affinity, but probably related to intrinsic crosslinking capacity of mAb. (+info)
Structural details of proteinase entrapment by human alpha2-macroglobulin emerge from three-dimensional reconstructions of Fab labeled native, half-transformed, and transformed molecules. (8/2903)Three-dimensional electron microscopy reconstructions of native, half-transformed, and transformed alpha2-macroglobulins (alpha2Ms) labeled with a monoclonal Fab Fab offer new insight into the mechanism of its proteinase entrapment. Each alpha2M binds four Fabs, two at either end of its dimeric protomers approximately 145 A apart. In the native structure, the epitopes are near the base of its two chisel-like features, laterally separated by 120 A, whereas in the methylamine-transformed alpha2M, the epitopes are at the base of its four arms, laterally separated by 160 A. Upon thiol ester cleavage, the chisels on the native alpha2M appear to split with a separation and rotation to give the four arm-like extensions on transformed alpha2M. Thus, the receptor binding domains previously enclosed within the chisels are exposed. The labeled structures further indicate that the two protomeric strands that constitute the native and transformed molecules are related and reside one on each side of the major axes of these structures. The half-transformed structure shows that the two Fabs at one end of the molecule have an arrangement similar to those on the native alpha2M, whereas on its transformed end, they have rotated. The rotation is associated with a partial untwisting of the strands and an enlargement of the openings to the cavity. We propose that the enlarged openings permit the entrance of the proteinase. Then cleavage of the remaining bait domains by a second proteinase occurs with its entrance into the cavity. This is followed by a retwisting of the strands to encapsulate the proteinases and expose the receptor binding domains associated with the transformed alpha2M. (+info)
Early abciximab administration before transfer for primary percutaneous coronary interventions for ST-elevation myocardial...
Background There are conflicting data on the clinical benefit from early administration of abciximab from a large randomized trial and a registry. However, both sources suggest that a benefit may depend on the baseline risk profile of the patients. We evaluated the role of early abciximab administration in patients with ST-segment-elevation myocardial infarction (STEMI) referred for primary percutaneous coronary intervention stratified by the STEMI Thrombolysis In Myocardial Infarction (TIMI) risk score. Methods A total of 1,650 patients were enrolled into the EUROTRANSFER Registry. One thousand eighty-six patients received abciximab (66%). Abciximab was administered early in 727 patients (EA) and late in 359 patients (LA). We used the TIMI risk score for risk stratification. Patients with scores 3 constituted the high-risk group of 616 patients (56.7%), whereas 470 patients formed the low-risk cohort. Factoring in the timing of the abciximab administration resulted in 4 groups of patients who ...
Recognition properties of a panel of human recombinant Fab fragments to the CD4 binding site of gp120 that show differing...
Six recombinant human Fab fragments that were derived from the same human immunodeficiency virus type 1 (HIV-1)-infected individual and are directed against the CD4 binding site (CD4bs) of the gp120 envelope glycoprotein were studied. A range of neutralizing activity against the HIV-1 (HXBc2) isolate was observed, with Fab b12 exhibiting the greatest potency among the Fabs tested. The neutralizing potency of Fab b12 was better than that of monoclonal whole antibodies directed against the third variable (V3) region of gp120. To explore the basis for the efficient neutralizing activity of b12, the recognition of a panel of HIV-1 gp120 mutants by the six Fabs was studied. The patterns of sensitivity to particular gp120 amino acid changes were similar for all six Fabs to those seen for anti-CD4bs monoclonal antibodies derived from HIV-1-infected individuals by conventional means. In addition, recognition by Fab b12 demonstrated an atypical sensitivity to changes in the V1 and V2 variable regions. ...
Prognostic impact of blood transfusion after primary angioplasty for acute myocardial infarction: analysis from the CADILLAC ...
Prognostic impact of blood transfusion after primary angioplasty for acute myocardial infarction: analysis from the CADILLAC (Controlled Abciximab and Device Investigation to Lower Late Angioplasty Complications) Trial.
SUV39H1 recombinant Fab fragment (Biotin) - (RAB00258) - Products - Abnova
Recombinant mouse Fab fragment raised against human SUV39H1. Original antibody is raised against recombinant protein corresponding to amino acids 42-100 of human SUV39H1. (RAB00258) - Products - Abnova
Beta-galactosidase recombinant Fab fragment - (RAB00031) - Products - Abnova
Recombinant His-tagged mouse Fab fragment raised against beta-galactosidase. Original antibody is raised against beta-galactosidase. (RAB00031) - Products - Abnova
Isolation of human Fab fragments against ovarian carcinoma using guided selection.<...
TY - JOUR. T1 - Isolation of human Fab fragments against ovarian carcinoma using guided selection.. AU - Figini, Mariangela. AU - Green, Andrew. AU - Colotta, Francesco. AU - Canevari, Silvana. PY - 2003. Y1 - 2003. UR - http://www.scopus.com/inward/record.url?scp=1842833210&partnerID=8YFLogxK. UR - http://www.scopus.com/inward/citedby.url?scp=1842833210&partnerID=8YFLogxK. M3 - Article. C2 - 12412473. AN - SCOPUS:1842833210. VL - 207. SP - 145. EP - 159. JO - Methods in Molecular Biology. JF - Methods in Molecular Biology. SN - 1064-3745. ER - ...
Recombinant Anti-Human tenascin c, st2146 Antibody Fab Fragment - Creative Biolabs
Recombinant Anti-Human tenascin c, st2146 Antibody Fab Fragment expressed in E. coli system can be provided from Creative Biolabs.
Recombinant Anti-Human phosphatidylserine Antibody Fab Fragment - Creative Biolabs
Recombinant Anti-Human phosphatidylserine Antibody Fab Fragment expressed in E. coli system can be provided from Creative Biolabs.
Screening of Human Antibody Fab Fragment against HBsAg and the Construction of its dsFv Form
1. Sidhu SS, Koide S. Phage display for engineering and analyzing protein interaction interfaces. Curr Opin Struct Biol. 2007;17:481-7 2. Brekke O.H, Loset G.A. New technologies in therapeutic antibody development. Curr Opin Pharmacol. 2003;3:544-50 3. Thullier P, Lafaye P, Megret F. et al. A recombinant Fab neutralizes dengue virus in vitro. J Biotechnol. 1999;69:183-90 4. Skerra A. Bacterial expression of immunoglobulin fragments. Curr Opin Immunol. 1993;5:256-62 5. Skerra A, Pluckthun A. Assembly of a functional immunoglobulin Fv fragment in Escherichia coli. Science. 1988;240:1038-41 6. Glockshuber R, Malia M, Pfitzinger I. et al. A comparison of strategies to stabilize immunoglobulin Fv-fragments. Biochemistry. 1990;29:1362-7 7. Kirsch M, Zaman M, Meier D. et al. Parameters affecting the display of antibodies on phage. J Immunol Meth. 2005;301:173-85 8. Marks JD, Hoogenboom HR, Griffiths AD. et al. Molecular evolution of proteins on filamentous phage. J Biol Chem. 1992;267:16007-10 9. Bird ...
Fab Antibody Production | Sino Biological
Learn information about Fab antibody fragment, covering definition, molecular weight, structure, expression, production, purification and services.
Abciximab Bolus Injection Does Not Reduce Cerebral Ischemic Complications of Elective Carotid Artery Stenting | Stroke
Abciximab has been shown to reduce ischemic complications in percutaneous coronary interventions. The effects were more consistent in patients who experienced unstable angina.7-10⇓⇓⇓ In a small, placebo-controlled study, abciximab was used in patients with recent stroke.11 Abciximab showed minimal improvement in clinical outcome with no complications exceeding placebo. In another case report, thrombotic complications during angioplasty of cerebral arteries could be successfully treated with abciximab.12,13⇓. According to these promising results, abciximab was used in several small, uncontrolled studies in patients undergoing carotid artery stenting.14-16⇓⇓ Chastain et al16 used abciximab in 23 high-risk patients prophylactically or intraprocedurally as a bailout therapy. The study did not include a control group. Qureshi et al15 administered abciximab only prophylactically in 20 high-risk procedures involving not only the carotid arteries but also the vertebral and basilar arteries. ...
EP 3182999 A1 20170628 - ANTI-LAG3 ANTIBODIES AND ANTIGEN-BINDING FRAGMENTS
479000617 - EP 3182999 A1 2017-06-28 - ANTI-LAG3 ANTIBODIES AND ANTIGEN-BINDING FRAGMENTS - [origin: WO2016028672A1] The present invention includes antibodies and antigen-binding fragments thereof that specifically bind to human or cynomolgous monkey LAG3 as well as immunoglobulin chains thereof and polynucleotides encoding the same along with injection devices comprising such antibodies or fragments. Vaccines including such antibodies and fragments as well as compositions comprising the antibodies and fragments (e.g., including anti-PD1 antibodies) are included in the invention. Methods for treating or preventing cancer or infection using such compositions are also provided. In addition, methods for recombinant expression of the antibodies and fragments are part of the present invention.[origin: WO2016028672A1] The present invention includes antibodies and antigen-binding fragments thereof that specifically bind to human or cynomolgous monkey LAG3 as well as immunoglobulin chains thereof and
Anti-Chicken IgY Fab Antibody | Donkey anti-Chicken Polyclonal FITC | LSBio
Chicken IgY Fab antibody LS-C750840 is an FITC-conjugated donkey polyclonal antibody to chicken Chicken IgY Fab. Validated for IHC.
Intraprocedural abciximab bolus versus pretreatment oral dual antiplatelet medication for endovascular stenting of unruptured...
Results There were 52 procedures in the pretreatment group and 47 in the abciximab group. More flow-diverting stents were placed in the pretreatment group than in the abciximab group (45 vs 23, p,0.001), and the aneurysm diameter was larger (11.2±6.7 vs 8.3±4.7 mm, p=0.01). There were 11 thrombotic and 7 access site complications, with no significant difference between the groups (p,0.99 and p=0.12, respectively). There were no intracranial hemorrhages. In patients with postoperative MRI, there was no difference in the presence of diffusion-restricted lesions between groups (p=0.20). Multivariate analysis of a composite of any complication did not show significant associations with aneurysm or patient variables in either group. ...
Clinical and demographic characteristics predictive of treatment outcomes for certolizumab pegol in moderate to severe Crohns...
These analyses identified several influential parameters for short-and long-term remission of Crohns disease with certolizumab pegol treatment. The data yield valuable hypotheses regarding factors that influence certolizumab pegol treatment. More investigation is needed. (ClinicalTrials.gov identifier NCT00552058).Predictors for initial remission (N = 377) included age, haematocrit, prior IBD surgery and entry HBI (P , 0.05 for all). Predictors for loss of remission (N = 437) included HBI, serum albumin concentration, haematocrit, smoking status and exposure. Predictors of maintenance of remission (N = 437) included haematocrit, IBD surgery, HBI, disease duration, serum albumin concentration and exposure. Significant predictors were confirmed with stepwise multivariate regression models.Patients who had completed placebo-controlled studies (PRECiSE 1/PRECiSE 2, P1/P2) enrolled in P3 and received open-label CZP 400 mg every 4 weeks up to 7 years. Baseline predictors included, but were not ...
Certolizumab pegol for the treatment of Crohns disease. | IKMB
In patients with moderate-to-severe Crohns disease, induction and maintenance therapy with certolizumab pegol was associated with a modest improvement in response rates, as compared with placebo, but with no significant improvement in remission rates. (ClinicalTrials.gov number, NCT00152490 [ClinicalTrials.gov].).Among patients with a baseline CRP level of at least 10 mg per liter, 37% of patients in the certolizumab group had a response at week 6, as compared with 26% in the placebo group (P=0.04). At both weeks 6 and 26, the corresponding values were 22% and 12%, respectively (P=0.05). In the overall population, response rates at week 6 were 35% in the certolizumab group and 27% in the placebo group (P=0.02); at both weeks 6 and 26, the response rates were 23% and 16%, respectively (P=0.02). At weeks 6 and 26, the rates of remission in the two groups did not differ significantly (P=0.17). Serious adverse events were reported in 10% of patients in the certolizumab group and 7% of those in the ...
Human-Derived Anti-Oxidized LDL Autoantibody Blocks Uptake of Oxidized LDL by Macrophages and Localizes to Atherosclerotic...
To understand the potential role(s) that autoantibodies to OxLDL play in atherogenesis in humans, we isolated several human monoclonal IgG Fab antibodies that bound to epitopes of OxLDL, initially focusing on MDA-LDL, a model epitope of OxLDL. To our knowledge, these are the first human monoclonal antibodies against oxidation-specific epitopes of OxLDL to be characterized. All 3 Fab antibodies showed distinct antigen-binding specificity to MDA-LDL compared with other unrelated antigens. To isolate these antibodies, we used a phage display combinatorial library technique that enables rapid enrichment of desired Fab clones by using antigen-coated surfaces.10 This technique has been used to isolate monoclonal Fab antibodies from immunized animals and to study pathogenic autoantibodies in various human autoimmune disorders.23-25 Although it is not feasible to directly compare hybridomas and combinatorial libraries, several studies have demonstrated that repertoires cloned from phage display ...
Cap formation - Wikipedia
When molecules on the surface of cell are crosslinked, they are moved to one end of the cell to form a cap. This phenomenon, the process of which is called cap formation, was discovered in 1971 on lymphocytes and is a property of amoebae and all locomotory animal cells except sperm. The crosslinking is most easily achieved using a polyvalent antibody to a surface antigen on the cell. Cap formation can be visualised by attaching a fluorophore, such as fluorescein, to the antibody. The antibody is bound to the cell. If the antibody is non-crosslinking (such as a Fab antibody fragment), the bound antibody is uniformly distributed. This can be done at 0 °C, room temperature, or 37 °C. If the antibody is crosslinking and bound to the cells at 0 °C, the distribution of antibodies has a patchy appearance. These patches are two-dimensional precipitates of antigen-antibody complex and are quite analogous to the three-dimensional precipitates that form in solution. If cells with patches are warmed ...
Baculo Expression Vector pAc-kappa-Fc - Phage Display - Products
|p||strong|Introduction|/strong||br /|Recombinant antibody technologies have been widely used to produce various single-chain Fv or Fab antibody fragments of different specificity. The randomized combination of cloned variable heavy and light chain imm
Induction of cell-cell adhesion by monovalent antibodies in a germ cell culture | Development
Four monoclonal antibodies, XT-I, MT-23, MT-24 and MT-29, that bind the XT-1-differentiation-antigen of male germ cells have been used to investigate the biological role of the XT-1-molecule of germ cells in short-term primary culture. Cultures from 10 days postpartum mice demonstrate increasing numbers of antigen-positive germ cells and increased antigen expression per cell with succeeding days of culture. Treatment of the antigen-positive cultures with three of the monoclonal antibodies, XT-I, MT-23 and MT-24, increases germ cell-germ cell adhesion in a dose-dependent fashion. Treatment with the fourth monoclonal antibody, MT-29, does not induce cell adhesion. The monovalent, Fab fragment of XT-I-antibody also elicits tight cell adhesion, thus ruling out antibody cross linking of molecules or cells. Saturating or near saturating amounts of the positive antibodies are required to produce adhesion, a result consistent with perturbation of a function that is performed by the sum of action of many ...
RCSB PDB - 6XCM: Structure of the SARS-CoV-2 spike glycoprotein in complex with the C105 neutralizing antibody Fab fragment ...
As a member of the wwPDB, the RCSB PDB curates and annotates PDB data according to agreed upon standards. The RCSB PDB also provides a variety of tools and resources. Users can perform simple and advanced searches based on annotations relating to sequence, structure and function. These molecules are visualized, downloaded, and analyzed by users who range from students to specialized scientists.
HuCAL Fab-Cys3H Negative Control Antibody, clone AbD14892 | Bio-Rad
|strong|HuCAL Fab-Cys3H negative control, clone AbD14892|/strong| is a recombinant Fab antibody fragment in the format Fab-Cys3H with specificity for green fluorescent protein (GFP). It has no known r…
A randomized, placebo-controlled trial of certolizumab pegol (CDP870) for treatment of Crohns disease
BACKGROUND & AIMS: To investigate the efficacy and safety of certolizumab pegol (a polyethylene-glycolated Fab fragment of anti-tumor necrosis factor
Accuracy and safety of <sup>99m</sup>Tc-labeled anti-D-dimer (DI-80B3) Fab fragments (ThromboView®) in the diagnosis of deep...
TY - JOUR. T1 - Accuracy and safety of 99mTc-labeled anti-D-dimer (DI-80B3) Fab fragments (ThromboView®) in the diagnosis of deep vein thrombosis. T2 - A phase II study. AU - Douketis, James D.. AU - Ginsberg, Jeffrey S.. AU - Haley, Susan. AU - Julian, Jim. AU - Dwyer, Miriam. AU - Levine, Mark. AU - Eisenberg, Paul R.. AU - Smart, Richard. AU - Tsui, Wendy. AU - White, Richard H. AU - Morris, Timothy A.. AU - Kaatz, Scott. AU - Comp, Philip C.. AU - Crowther, Mark A.. AU - Kearon, Clive. AU - Kassis, Jeannine. AU - Bates, Shannon M.. AU - Schulman, Sam. AU - Desjardins, Louis. AU - Taillefer, Raymond. AU - Begelman, Susan M.. AU - Gerometta, Mike. PY - 2012/9. Y1 - 2012/9. N2 - Background: The assessment of patients with suspected deep vein thrombosis (DVT) remains challenging despite current diagnostic algorithms. 99mTc-labelled DI-DD3B6/22-80B3 Fab́ fragments ( 99mTc-DI-80B3, ThromboView®) is a novel diagnostic test that uses a radiolabelled humanized monoclonal antibody fragment ...
Study to Test the Safe and Effective Use of an e-Device for the Self-injection of Certolizumab Pegol Solution by Subjects With...
Subjects must have been prescribed Certolizumab Pegol (CZP) and must have been self-injecting CZP using the pre-filled syringe for at least 3 months prior to Visit 1. Subjects with RA, PsA, or AS must have been on a stable Q2W (every 2 weeks) or Q4W (every 4 weeks) CZP dosing regimen for at least 3 months prior to Screening. Subjects with CD must have been on a stable Q4W CZP dosing regimen for at least 3 months prior to Visit 1 ...
Human IgG F(ab)2 Antibody 209-1104
Anti-Human IgG F(ab)2 Antibody generated in goat recognizes the dimeric Fab portion of the human IgG molecule. Human IgG F(ab)2 is a proteolytic fragment of immunoglobulin G (IgG) obtained by limited digestion with the enzyme pepsin under controlled conditions of temperature, time and pH. F(ab)2 molecules lack the Fc portion of IgG and therefore receptors that bind human IgG F(c) will not bind human IgG F(ab)2 molecules. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.
Certolizumab pegol for the treatment of rheumatoid arthritis | Guidance | NICE
This guidance has been updated and replaced by adalimumab, etanercept, infliximab, certolizumab pegol, golimimab, tocilizumab and abatacept for rheumatoid
UCB, Inc.-Sponsored Data on Cimzia® (certolizumab pegol) to be Highlighted at 2013 American College of Rheumatology Annual...
UCB, Inc.-Sponsored Data on Cimzia® (certolizumab pegol) to be Highlighted at 2013 American College of Rheumatology Annual Scientific Meeting - read this article along with other careers information, tips and advice on BioSpace
New Data Presented From WELCOME Trial Show Positive Impact of CIMZIA(R) (certolizumab pegol) on Hospitalizations and Surgeries...
According to new results from the WELCOME trial, exploratory data analyzing the impact of treatment with CIMZIA( ) (certolizumab pegol) - the only PEGylated anti-TNF (alpha) (Tumor
A Phase 2, Open-label, Multicenter Study to Assess the Safety and Efficacy of Certolizumab Pegol in Children and Adolescents...
This trial was about to investigate the efficacy of induction certolizumab pegol [Cimzia, UCB] in children and adolescents with active Crohns disease.
Physical and Functional Bivalency Observed Among TCR/CD3 Complexes Isolated from Primary T Cells | The Journal of Immunology
Although we have shown that bivalent TCR/CD3 are present among digitonin-solubilized complexes, we do not know the proportion of complexes that they represent. One focus of future experiments must be to develop other methods that would permit a quantitative estimate of the prevalence of bivalency among all TCR/CD3 complexes. However, despite this current technical limitation, the data in Fig. 7 imply that the proportion of bivalent complexes is sufficiently high to impact the outcome of the pMHC-Ig fusion protein binding assay performed. Thus, based on the assumption that this assay reflects a true potential for functional impact, we speculate that it is likely that a biologically significant number of TCR/CD3 complexes display bivalency.. It is not known which motifs might specifically interact to compose a bivalent complex. Even in the standard monovalent model of TCR/CD3, the interactions between subunit dimers (αβ/εγ/εδ/ζζ) that compose the multiprotein complex are not fully ...
Certolizumab Pegol (Cimzia) use while Breastfeeding
Advice for mothers using Certolizumab Pegol (Cimzia) while breastfeeding. Includes possible effects on breastfed infants and lactation.
RCSB PDB - 3HC0: BHA10 IgG1 wild-type Fab - antibody directed at human LTBR
As a member of the wwPDB, the RCSB PDB curates and annotates PDB data according to agreed upon standards. The RCSB PDB also provides a variety of tools and resources. Users can perform simple and advanced searches based on annotations relating to sequence, structure and function. These molecules are visualized, downloaded, and analyzed by users who range from students to specialized scientists.
Monoclonal Antibody Fragment Separation and Characterization Using Size Exclusion Chromatography Coupled with Mass Spectrometry...
Monoclonal ntibody Fragment Separation and haracterization Using Size Exclusion hromatography oupled with Mass Spectrometry uthors Haiying hen Katherine McLaughlin Sepax Technologies, Inc. 5 Innovation
Anti-BACE2 [1/ 9]
Banner DW, Gsell B, Benz J, Bertschinger J, Burger D, Brack S, Cuppuleri S, Debulpaep M, Gast A, Grabulovski D, Hennig M, Hilpert H, Huber W, Kuglstatter A, Kusznir E, Laeremans T, Matile H, Miscenic C, Rufer AC, Schlatter D, Steyaert J, Stihle M, Thoma R Mapping the conformational space accessible to BACE2 using surface mutants and cocrystals with Fab fragments, Fynomers and Xaperones. Acta Crystallogr D Biol Crystallogr. 2013 Jun;69(Pt 6):1124-37 PMID:23695257 Note on publication: Describes the role of BACE2 as a potential therapeutic target for the pathogenesis of diabetes and Alzheimers Disease. Also describes the characterization of the crystal structure of BACE2 with the aid of BACE2-binding antibody Fab fragments. Reviews: No reviews available for this antibody yet. Be the first to submit a review at pAbmAbs and enter into their monthly prize draw ...
A novel LFA-1 activation epitope maps to the I domain. | Journal of Cell Biology | Rockefeller University Press
A panel of 21 alpha-subunit (CD11a) and 10 beta-subunit (CD18) anti-LFA-1 mAbs was screened for ability to activate LFA-1. A single anti-CD11a mAb, MEM-83, was identified which was able to directly induce the binding of T cells to purified ICAM-1 immobilized on plastic. This ICAM-1 binding could be achieved by monovalent Fab fragments of mAb MEM-83 at concentrations equivalent to whole antibody, was associated with appearance of the activation reporter epitope detected by mAb 24, and was completely inhibited by anti-ICAM-1 and LFA-1 blocking mAbs. The epitope recognized by mAb MEM-83 was distinct from that recognized by mAb NKI-L16, an anti-CD11a mAb previously reported to induce LFA-1 activation, in that it was constitutively present on freshly isolated peripheral blood mononuclear cells and was not divalent cation dependent for expression. The ICAM-1 binding activity induced by mAb MEM-83 was, however, dependent on the presence of Mg2+ divalent cations. Using an in vitro-translated CD11a ...
Rabbit IgG antibody, Fab fragment (Rhodamine) | GeneTex
, Rabbit IgG antibody, Fab fragment (Rhodamine), GTX27051, Applications: ELISA, FACS, ICC/IF; ELISA, Flow cytometry/FACS, Immunocytochemistry/ Immunofluorescence (ICC/IF); CrossReactivity: Rabbit
Anti Mouse IgG Fab Affinity Purified Antibody | Bio-Rad
|strong|Goat anti mouse IgG Fab antibody|/strong| recognizes mouse IgG Fab fragment. This antibody will also react with light chains from other mouse immunoglobulins and may cross-react with Fab frag…
Sequencing and Quantifying IgG Fragments and Antigen-Binding Regions by Mass Spectrometry | Bioinformatics Solutions Inc.
de Costa, D., et al. Sequencing and Quantifying IgG Fragments and Antigen-Binding Regions by Mass Spectrometry. J Proteome Res. 9(6), 2937-45. 04/06/2010.. ...
Angioplasty Complications - Health Hearty
For people affected by heart diseases, angioplasty is a life-saver. The complications involved in this procedure are very rare, but in case they occur, they can sometimes prove to be life-threatening. This article provides some information on the complications involved with this procedure.
What Is Pharmacology - Abciximab - Medicalrealm
Abciximab is an anti platelet anti thrombotic drug. The half life of abciximab is 10 - 30 minute. Abciximab is useful as an additional/ adjunct therapy with aspirin and heparin in patient ( high risk) while undergoing clinical procedure such as coronary a
4LVO CRYSTAL STRUCTURE OF PFSUB1-PRODOMAIN-NIMP.M7 FAB COMPLEX WITH ADDED CACL2 | 4LVO B | P15056 | BRAF | Serine/threonine...
Fullscreen (supported by IE11, latest versions of Firefox, Chrome, Safari (not including iOS Safari), Edge, Chrome for Android, Samsung Internet) ...
Overview of Cimzia | Crohns Disease Forum
Certolizumab pegol (Cimzia) Certolizumab pegol (Cimzia) is a pegylated humanized antibody fragment also directed against TNF alpha, acting similarly to...
Certolizumab pegol injection
Visit your doctor or health care professional for regular checks on your progress. Tell your doctor or healthcare professional if your symptoms do not start to get better or if they get worse. Your condition will be monitored carefully while you are receiving this medicine.. You will be tested for tuberculosis (TB) before you start this medicine. If your doctor prescribes any medicine for TB, you should start taking the TB medicine before starting this medicine. Make sure to finish the full course of TB medicine.. Call your doctor or health care professional for advice if you get a fever, chills, sore throat, or other symptoms of an infection. Do not treat yourself. This medicine may decrease your bodys ability to fight infection. Try to avoid being around people who are sick.. Talk to your doctor about your risk of cancer. You may be more at risk for certain types of cancers if you take this medicine.. ...
Support During COVID‐19 ‐ CIMZIA® (certolizumab pegol)
Find resources and support to help you manage your CIMZIA treatment plan during the coronavirus pandemic. See full prescribing information including boxed warning on serious infections.
Idarucizumab | SeekHealthZ
Idarucizumab is a humanized monoclonal antibody fragment indicated for dabigatran-treated patients when reversal of the anticoagulant effects of dabigatran is
Chemical Biology Seminar | Caltech
Due to their specificity and favorable pharmacological properties, monoclonal antibodies (mAbs) are being rapidly developed and armed to image and treat disease. Invariably, chemical methods and/or extensive genetic engineering efforts are required to add novel functionality to mAbs. Through diffraction methods, we have identified a novel peptide interface within a cavity formed by the light and heavy chains of the cetuximab Fab that may provide an entirely novel, noncovalent route to functionalize and/or manipulate mAbs. Distinct from other Fab binding proteins such as protein A or protein L, we show that this peptide interaction is exclusive to cetuximab. In other words, the peptide binding site absent in human mAbs. Moreover, we demonstrate that the presence of the peptide (which we have named a meditope) does not affect antigen binding. Anticipating that this non-covalent method could be used to direct an imaging agent to a tumor cell, we created a bivalent meditope analog and show that this ...
Intracoronary Thrombus and Platelet Glycoprotein IIb/IIIa Receptor Blockade With Tirofiban in Unstable Angina or Non-Q-Wave...
This angiographic study in PRISM-PLUS was the first to examine the effects of platelet GP IIb/IIIa receptor blockade on the culprit lesion in patients with UA or NQWMI. It was also the largest prospective study that examined the angiographic characteristics of culprit lesions and their prognostic significance. The study demonstrated that the combination of tirofiban, heparin, and aspirin significantly reduced the thrombus burden of the culprit lesion by 23% beyond the effects of heparin and aspirin, resulting in decreased coronary obstruction and improved distal flow. These data are consistent with the 32% reduction in risk of death, MI, or RI observed at 7 days with the combination therapy and with the 43% reduction in the risk of death or MI. The present study also provided evidence that persistence of an angiographic thrombus after a course of medical therapy is an indicator of a worse prognosis, which suggests important pathophysiological concepts and therapeutic implications.. The ...
Standard versus low-dose weight-adjusted heparin in patients treated with the platelet glycoprotein IIb/IIIa receptor antibody...
Standard versus low-dose weight-adjusted heparin in patients treated with the platelet glycoprotein IIb/IIIa receptor antibody fragment Abciximab (c7E3 Fab) during percutaneous coronary revascularization Academic Article Article ...
Influence of growth temperature on the production of antibody Fab fragments in different microbes: A host comparative analysis<...
TY - JOUR. T1 - Influence of growth temperature on the production of antibody Fab fragments in different microbes: A host comparative analysis. AU - Dragosits, Martin. AU - Frascotti, Gianni. AU - Bernard-Granger, Lise. AU - Vázquez, Felícitas. AU - Giuliani, Maria. AU - Baumann, Kristin. AU - Rodríguez-Carmona, Escarlata. AU - Tokkanen, Jaana. AU - Parrilli, Ermenegilda. AU - Wiebe, Marilyn G.. AU - Kunert, Renate. AU - Maurer, Michael. AU - Gasser, Brigitte. AU - Sauer, Michael. AU - Branduardi, Paola. AU - Pakula, Tiina. AU - Saloheimo, Markku. AU - Penttilä, Merja. AU - Ferrer, Pau. AU - Luisa Tutino, Maria. AU - Villaverde, Antonio. AU - Porro, Danilo. AU - Mattanovich, Diethard. PY - 2011/1/1. Y1 - 2011/1/1. N2 - Microorganisms encounter diverse stress conditions in their native habitats but also during fermentation processes, which have an impact on industrial process performance. These environmental stresses and the physiological reactions they trigger, including changes in the ...
Evidence for Prevention of Death and Myocardial Infarction With Platelet Membrane Glycoprotein IIb/IIIa Receptor Blockade by...
Despite considerable research into pharmacologic and revascularization treatments for the acute coronary ischemic syndromes, outcome among patients hospitalized with unstable angina remains unsatisfactory. Recently reported large-scale randomized trials, for example, have demonstrated that the risk of death or MI within the first 4 to 6 weeks after development of unstable angina is as high as 9% to 11% ([27, 28]). Although percutaneous myocardial revascularization has been advocated as a means of reducing morbidity in this group of patients, particularly those with symptoms refractory to medical therapy (), several studies have suggested that patients undergoing a coronary intervention in the setting of unstable angina are at elevated risk for ischemic complications compared with patients treated by revascularization for more stable indications ([5-8]).. The current study evaluated clinical outcome in the subgroup of 489 patients who met rigorous criteria for unstable angina in the ...
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جامعة الأندلس الخاصة للعلوم الطبية - Cimzia (Certolizumab Pegol Injection)
Patients were tested at multiple time points for antibodies to certolizumab pegol during Studies CD1 and CD2. The overall percentage of antibody positive patients was 8% in patients continuously exposed to CIMZIA, approximately 6% were neutralizing in vitro. No apparent correlation of antibody development to adverse events or efficacy was observed. Patients treated with concomitant immunosuppressants had a lower rate of antibody development than patients not taking immunosuppressants at baseline (3% and 11%, respectively). The following adverse events were reported in Crohns disease patients who were antibody-positive (N = 100) at an incidence at least 3% higher compared to antibody-negative patients (N = 1,242): abdominal pain, arthralgia, edema peripheral, erythema nodosum, injection site erythema, injection site pain, pain in extremity, and upper respiratory tract infection.. The overall percentage of patients with antibodies to certolizumab pegol detectable on at least one occasion was 7% ...
High frequency of antidrug antibodies and association of random drug levels with efficacy in certolizumab pegol-treated...
This study demonstrates for the first time that certolizumab ADAbs were detectable in 37% of patients with RA over 12 months of treatment. Detectable ADAbs were associated with lower certolizumab drug concentrations, but not independently with treatment response. However, higher certolizumab drug levels were associated with better 12 months EULAR response. Following adjustment, ADAb concentrations and biologic adherence remained the most important predictors of drug levels over time.. Our data demonstrates that even small, non-glycosylated fragments such as certolizumab can be immunogenic. The higher levels of ADAbs detected compared with previous certolizumab trials1 ,2 is noteworthy. However, in contrast to other biologics, ADAbs against certolizumab may be detected more easily even in the presence of drug. Certolizumab is a Fab fragment, monovalent, and therefore, drug-ADAb complexes easily dissociate and can thus be detected despite the drug not necessarily being more immunogenic. RIA, for ...
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Examining the Long Term Safety, Efficacy, and Corticosteroid-sparing Effect of Certolizumab Pegol in Crohn's Disease -...
Certolizumab pegol (CDP870), an anti-tumor necrosis factor (TNF)α, humanized antibody Fab fragment - polyethylene glycol conjugate, solution for injection, in 10 mM sodium acetate buffer and 125 mM sodium chloride, pH 4.7, supplied in 3 mL vials with a fill of 1.4 mL (an extractable volume of 1 mL corresponds to a dose of 200 mg).. Dosing is every 4 weeks from Week 2 until Week 34, or until CDP870 is available for a Crohns disease indication in the patients country. Subjects who were Non-completers of C87059 (COSPAR I, NCT00349752) receive an additional CDP870 400 mg dose at Week 2. ...
Lack of Benefit From Intravenous Platelet Glycoprotein IIb/IIIa Receptor Inhibition as Adjunctive Treatment for Percutaneous...
Thank you for your interest in spreading the word on Circulation.. NOTE: We only request your email address so that the person you are recommending the page to knows that you wanted them to see it, and that it is not junk mail. We do not capture any email address. ...
Real-life experience with the specific reversal agent idarucizumab for the management of emergency situations in dabigatran...
Non-vitamin K antagonist oral anticoagulants (NOACs) have a favorable benefit-risk profile compared with vitamin K antagonists. However, the lack of specific reversal agents has made the management of some patients receiving long-term treatment with NOACs problematic in emergency situations such as major bleeding events or urgent procedures. Idarucizumab, a fully humanized Fab antibody fragment that binds specifically and with high affinity to dabigatran, was recently approved for use in adult patients treated with dabigatran when rapid reversal of its anticoagulant effect is required. Clinical experience with idarucizumab is currently limited. We report 11 real-life clinical cases in which idarucizumab was used after multidisciplinary consultation in a variety of emergency situations including severe postoperative bleeding, emergency high-bleeding-risk surgery (hip/spine surgery and neurosurgery), invasive diagnostic testing (lumbar puncture), intracranial bleeding (pre-pontine subarachnoid ...
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The Efficacy of Certolizumab Pegol over 4 Years in Psoriatic Arthritis Patients With and Without Concomitant Use of DMARDs |...
|p|In RAPID-PsA (NCT01087788) certolizumab pegol (CZP) improved signs and symptoms of psoriatic arthritis (PsA) over 4-years treatment.|/p|
certolizumab pegol | Ligand page | IUPHAR/BPS Guide to PHARMACOLOGY
The IUPHAR/BPS Guide to Pharmacology. certolizumab pegol ligand page. Quantitative data and detailed annnotation of the targets of licensed and experimental drugs.
Certolizumab pegol biosimilar - PanPharmaceuticals USA - AdisInsight
PanPharmaceuticals USA was developing a biosimilar of certolizumab pegol [see RDI profile 800010395], for the treatment of autoimmune diseases. The humanised
Engineering an Improved IgG4 Molecule with Reduced Disulfide Bond Heterogeneity and Increased Fab Domain Thermal Stability -...
The integrity of antibody structure, stability, and biophysical characterization are becoming increasingly important as antibodies receive increasing scrutiny from regulatory authorities. We altered the disulfide bond arrangement of an IgG4 molecule by mutation of the Cys at the N terminus of the heavy chain constant domain 1 (CH1) (Kabat position 127) to a Ser and introduction of a Cys at a variety of positions (positions 227-230) at the C terminus of CH1. An inter-LC-CH1 disulfide bond is thus formed, which mimics the disulfide bond arrangement found in an IgG1 molecule. The antibody species present in the supernatant following transient expression in Chinese hamster ovary cells were analyzed by immunoblot to investigate product homogeneity, and purified product was analyzed by a thermofluor assay to determine thermal stability. We show that the light chain can form an inter-LC-CH1 disulfide bond with a Cys when present at several positions on the upper hinge (positions 227-230) and that such ...
New RAPID 1 Post-Hoc Analysis Showed Early Response to Cimzia® (certolizumab pegol) at Week 12 was Predictive of Week 5... (...
...BRUSSELS and CHICAGO Nov. 7 2011 /- UCB today announced... This post-hoc analysis is consistent with previous evidence that sugg...Both RAPID3 and EULAR response criteria are measures of disease activi...The RAPID3 patient-derived assessment of disease activity has been sho...,New,RAPID,1,Post-Hoc,Analysis,Showed,Early,Response,to,Cimzia®,(certolizumab,pegol),at,Week,12,was,Predictive,of,Week,52,Response,medicine,advanced medical technology,medical laboratory technology,medical device technology,latest medical technology,Health
Complementarity determining region-independent recognition of a superantigen by B-cell antigen receptors of mantle cell...
This raised the question as to whether SpA might act as a superantigen, triggering activation of the BCR signaling pathway in a substantial proportion of MCL patients.. Therefore, we explored the interaction of SpA with lymphoma BCR-derived immunoglobulins harboring the SpA binding motif. Since SpA has a high affinity to the Fc domain of human IgG, representative MCL Igs were expressed as Fab fragments. Six different MCL- (with and without SpA binding motif), two CLL- and one FL-derived Fab fragments were produced and tested for SpA reactivity using an ELISA with coated SpA. In fact, all Fab fragments exhibiting the SpA binding motif bound to SpA in this assay, whereas Fab fragments without SpA binding motif were non-reactive (Figure 1B).. Having established that SpA binds to a substantial proportion of MCL-derived immunoglobulins in vitro, we next investigated whether this interaction is sufficient to activate the BCR signaling pathway in human B-cells expressing SpA-reactive MCL BCR. We ...
Peroxidase Conjugated Affinity Purified Anti-SHEEP IgG F(ab)2 (RABBIT)
Peroxidase Conjugated Affinity Purified Anti-SHEEP IgG F(ab)2 (RABBIT), Peroxidase Conjugated Affinity Purified anti-Sheep IgG F(ab )2 [Rabbit]; N/A Peroxidase Conjugated Affinity Purified Anti-SHEEP IgG F(ab)2 (RABBIT)IGHG1
Goat IgG antibody, Fab fragment (HRP) | GeneTex
, Goat IgG antibody, Fab fragment (HRP), GTX26667, Applications: ELISA, IHC, WB; ELISA, Immunohistochemistry (IHC), Western Blot (WB); CrossReactivity: Goat
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segment 44 - Precipitin Reactions • Insoluble lattice of Ab and Ag • Ab must be bivalent or polyvalent • Fab fragments will not...
View Notes - segment_44 from MMG 451 at Michigan State University. Precipitin Reactions • Insoluble lattice of Ab and Ag. • Ab must be bivalent or polyvalent: • Fab fragments will not work. •
Antibody Deficiencies | The Online Metabolic and Molecular Bases of Inherited Disease | OMMBID | McGraw-Hill Medical
Antibodies are produced by plasma cells, the terminally differentiated descendants of the B-cell lineage. The early stages of B-cell development occur in the bone marrow, where pro-B cells, which are derived from hematopoietic stem cells, undergo rearrangement of the immunoglobulin heavy-chain genes. This process occurs throughout the life of the individual. At the next stage of B-cell differentiation, in pre-B cells, there is rearrangement of the light-chain genes. This allows the expression of the intact immunoglobulin molecule on the cell surface of the B cell. Each B cell and its progeny express only one rearranged immunoglobulin heavy chain and one light chain. When antigen binds to the surface of the B cell, if the appropriate environmental signals are received, the B cell proliferates and differentiates into a memory B cell that can respond more rapidly to future exposures to that antigen or to a plasma cell that secretes high concentrations of antibodies.. ...
cel s bind to the Fc portion of the antibody, a signal ing cascade is Targeting cancer with a mAb was described by Milstein in initiated to kil the cancer cel s. However, the Fc domain of an intact 198123. Over the past two decades, the feasibility of antibody-based mAb can also bind to the Fc receptors on normal cel s, as occurs with tissue targeting has been clinical y demonstrated (reviewed in macrophages. This may lead to increased immunogenicity - the refs 24,25) with 17 different mAbs approved by the US Food and ability to evoke an immune response - and liver and spleen uptake of Drug Administration (FDA)26. The mAb rituximab (Rituxan) was the nanocarrier. An additional advantage of whole/intact antibodies approved in 1997 for treatment of patients with non-Hodgkins is their ability to maintain stability during long-term storage. lymphoma - a type of cancer that originates in lymphocytes27. Although antibody fragments including antigen-binding fragments A year later, Trastuzumab ...
2010 Cowboy Cadillac Roamer 4X4 Series M-Ford 250hp Gas Prices, Values & Specs, 2010 Cowboy Cadillac Roamer 4X4 Series M-Ford...
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2012 BMW 328i Sport vs. 2013 Cadillac ATS 2.0 Turbo
Cadillac smartly realizes BMW rules the 3 Series segment, and looking over the spec sheet for the ATS, its clear Cadillac engineers used BMW specs as they would a recipe for their grandmothers lasagna. Read on to learn more on the 2012 BMW 328i Sport going up against the 2013 Cadillac ATS Turbo in this comparison brought to you by the automotive experts at Motor Trend.
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First phase 3 study evaluating Cimzia® (certolizumab pegol) in early rheumatoid arthritis presented at EULAR 2014 | MedTrials -...
Phase I (one) medical and clinical trial recruitment. Volunteer to join trials or advertise a clinical trial or medical study on MedTrials.
Scalable High Throughput Selection From Phage-displayed Synthetic Antibody Libraries | Protocol (Translated to Swedish)
En metod beskrivs med visuell ackompanjemang för att bedriva skalbara, hög genomströmning val från faguppvisade kombi syntetiska...
All Wales Medicines Strategy Group (AWMSG) - certolizumab pegol (Cimzia)
AWMSG ADVICE SUPERSEDED BY NICE GUIDANCE (TA445) NICE GUIDANCE ISSUED MAY 2017 (Refer to NICE website for full guidance on NICE recommendations, including any specific restrictions on the use of the technology ...
Abstract P-45: Structural Studies of Multispecific Antibody/Antigen Complexes by Cryo-EM | International Journal of Biomedicine
Background: Multispecific antibodies are artificially engineered molecules designed to bind simultaneously to several (different) antigens. Potential advantages of generating viable multispecific antibodies include the identification of malignant cells coupled with the concurrent recruitment of immune cells and the blocking of complex viral escape mechanisms. The cross-over dual-variable immunoglobulin (CODV-Ig) has been proposed as a universal bispecific therapeutic format. Its unique antigen-binding fragment (Fab) architecture provides pM affinities for ligands, no positional effect in target binding and a stable self-supporting structure. However, the three-dimensional arrangement of the constant and antigen-binding fragments in the CODV-Ig format may play a role in its in vivo effects. To further understand the structure and function of multispecific antibodies based on the CODV-Ig format high-resolution structural information is required. Towards this, we use cryo-electron microscopy ...
Anti-Myogenin antibody [F5D] (ab1835) | Abcam
Mouse monoclonal Myogenin antibody [F5D] validated for WB, IHC, Flow Cyt, ICC/IF and tested in Human, Mouse and Rat. Referenced in 29 publications and 7…
Anti-Myogenin antibody [F5D] (ab1835) References | Abcam
Anti-Myogenin antibody [F5D] (ab1835) has been cited in 29 publications. References for Human, Mouse, Rat, Cow, Cat in ICC, ICC/IF, IF, IHC, IHC-Fr, IHC-P, WB
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Cadillac CTS-V is the first sedan to reach 200mph without mods
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... is made from the Fab fragments of an immunoglobulin that targets the glycoprotein IIb/IIIa receptor on the platelet ...
1996). "Crystallization of a complex between the Fab fragment of a human immunoglobulin M (IgM) rheumatoid factor (RF-AN) and ... Immunoglobulin lambda-like polypeptide 1 is a protein that in humans is encoded by the IGLL1 gene. IGLL1 has also recently been ... 1989). "Immunoglobulin lambda light-chain-related genes 14.1 and 16.1 are expressed in pre-B cells and may encode the human ... 1991). "The immunoglobulin lambda-like gene cluster (14.1, 16.1 and F lambda 1) contains gene(s) selectively expressed in pre-B ...
List of MeSH codes (D12.776.124)
... immunoglobulin fab fragments MeSH D12.776.124.486.485.538.500 - immunoglobulin fc fragments MeSH D12.776.124.486.485.538. ... immunoglobulin fab fragments MeSH D12.776.124.790.651.538.500 - immunoglobulin fc fragments MeSH D12.776.124.790.651.538. ... immunoglobulin fragments MeSH D12.776.124.486.485.680.650 - immunoglobulin fab fragments MeSH D12.776.124.486.485.680.650.500 ... immunoglobulin fab fragments MeSH D12.776.124.486.485.797.590 - immunoglobulin joining region MeSH D12.776.124.486.485.900 - ...
Digoxin immune fab
DigiFab is a sterile, lyophilized preparation of digoxin-immune ovine Fab (monovalent) immunoglobulin fragments. It is prepared ... papain is used to cleave the antibody into Fab and Fc fragments) should not use ovine digoxin immune fab. Because it is ... digesting it with papain and isolating the digoxin-specific Fab fragments by affinity chromatography. These antibody fragments ... Digoxin immune fab or digoxin-specific antibody is an antidote for overdose of digoxin. It is made from immunoglobulin ...
The primary treatment of digoxin toxicity is digoxin immune fab, which is an antibody made up of anti-digoxin immunoglobulin ... "Treatment of 150 cases of life-threatening digitalis intoxication with digoxin-specific Fab antibody fragments. Final report of ... Fab dose can be determined by two different methods. First method is based on the amount of digoxin ingested whereas the second ... Treatment of severe toxicity is with digoxin-specific antibody fragments. Its use is recommended in those who have a serious ...
The enzyme papain can be used to cleave an immunoglobulin monomer into two Fab fragments and an Fc fragment. Conversely, the ... two Fab fragments and one Fc fragment An antibody digested by pepsin yields two fragments: a F(ab')2 fragment and a pFc' ... which is only half the size of the Fab fragment, yet retains the original specificity of the parent immunoglobulin. Fabs have ... The F(ab')2 fragment can be split into two Fab' fragments by mild reduction. Heavy and light chains, variable and constant ...
Immunoglobulin heavy constant alpha 1
Streptococcal IgA1 protease, digestion, Fab and Fc fragments, and the complete amino acid sequence of the alpha 1 heavy chain ... Streptococcal IgA1 protease, digestion, Fab and Fc fragments, and the complete amino acid sequence of the alpha 1 heavy chain ... Immunoglobulin heavy constant alpha 1 is a immunoglobulin gene with symbol IGHA1. It encodes a constant (C) segment of ... Immunoglobulin A is an antibody that plays a critical role in immune function in the mucous membranes. IgA shows the same ...
... fragments, such as Fab and nanobodies are not considered as antibody mimetics. Common advantages over antibodies are ... Each immunoglobulin domain has a similar structure, characteristic of all the members of the immunoglobulin superfamily: it is ... Streptococcal IgA1 protease, digestion, Fab and Fc fragments, and the complete amino acid sequence of the alpha 1 heavy chain ... Somatic recombination of immunoglobulins, also known as V(D)J recombination, involves the generation of a unique immunoglobulin ...
Fragment crystallizable region
This type of antigen-binding fragment is called Fcab. Fcab fragments can be inserted into a full immunoglobulin by swapping the ... Antibody Fab region Protein tag Janeway, CA, Jr.; et al. (2001). Immunobiology (5th ed.). Garland Publishing. ISBN 978-0-8153- ... "Introducing antigen-binding sites in structural loops of immunoglobulin constant domains: Fc fragments with engineered HER2/neu ... The fragment crystallizable region (Fc region) is the tail region of an antibody that interacts with cell surface receptors ...
1992). "Crystal structure of a chimeric Fab' fragment of an antibody binding tumour cells". J. Mol. Biol. 227 (1): 253-64. doi: ... Immunoglobulin kappa constant, also known as IGKC, is a human gene that encodes the constant domain of kappa-type light chains ... 2001). "Structure of CD40 ligand in complex with the Fab fragment of a neutralizing humanized antibody". Structure. 9 (4): 321- ... The primary structure of a monoclonal immunoglobulin L-chain of kappa-type, subgroup 3 (Bence-Jones protein Ti). IV. The ...
... two Fab fragments and one Fc fragment. An antibody can also be digested by pepsin to produce two fragments: a F(ab')2 fragment ... Whole antibody products consist of the entire antibody molecule, often immunoglobulin G (IgG), whereas antibody fragments are ... The fragment antigen-binding (Fab fragment) is a region on an antibody that binds to antigens, such as venoms. The molecular ... or Fab, is the selective antigen binding region. An antibody, such as IgG, can be digested by papain to produce three fragments ...
In an antibody, the Fab (fragment, antigen-binding) region is formed from the amino-terminal end of both the light and heavy ... "Immunoglobulins- antigen-antibody reactions and selected tests". Microbiology and Immunology. University of South Carolina ... chains of the immunoglobulin polypeptide. This region, called the variable (V) domain, is composed of amino acid sequences that ...
"Primary structure of a human IgA1 immunoglobulin. IV. Streptococcal IgA1 protease, digestion, Fab and Fc fragments, and the ... Antibody fragments, such as Fab and nanobodies are not considered as antibody mimetics. Common advantages over antibodies are ... Structurally an antibody is also partitioned into two antigen-binding fragments (Fab), containing one VL, VH, CL, and CH1 ... Somatic recombination of immunoglobulins, also known as V(D)J recombination, involves the generation of a unique immunoglobulin ...
In parallel with this work, Huber solved the structures of several immunoglobulin fragments. He was the first to determine the ... which was also the first variable and the first constant domains in Fab-fragments. Huber's structure of citrate synthase ... Colman, P. M.; Deisenhofer, J; Huber, R (1976). "Structure of the human antibody molecule Kol (immunoglobulin G1): An electron ... "Crystallographic structure studies of an IgG molecule and an Fc fragment". Nature. 264 (5585): 415-20. Bibcode:1976Natur.264.. ...
... which is missing in the Fab fragment. In case the IgG immunoglobulin was more suitable for the treatment or some other ... Fab fragment antibodies can be used for detection of not bound drugs or free drugs in the serum. Fab antibodies have also been ... Both scFv and Fab fragment recombinant antibodies are routinely produced using the antibody phage display. From all the ... Structurally Fab fragments consist of two sets of variable and constant components, which create two polypetide chains. ...
1992). "Crystal structure of a chimeric Fab' fragment of an antibody binding tumour cells". J. Mol. Biol. 227 (1): 253-64. doi: ... "Entrez Gene: IGHG1 immunoglobulin heavy constant gamma 1 (G1m marker)". Ponstingl H, Hilschmann N (1977). "[The rule of ... 2001). "Structure of CD40 ligand in complex with the Fab fragment of a neutralizing humanized antibody". Structure. 9 (4): 321- ... Deisenhofer J (1981). "Crystallographic refinement and atomic models of a human Fc fragment and its complex with fragment B of ...
Primary and secondary antibodies
"F(ab')₂ Fragment Secondary Antibodies - Jackson ImmunoResearch". www.jacksonimmuno.com. Retrieved 2021-01-29. "Fab Fragment ... yielding two monovalent Fab moieties. They can be used to block endogenous immunoglobulins on cells, tissues or other surfaces ... Papain digestion generates Fab fragments, which removes the entire Fc fragment including the hinge region, ... F(ab')2 fragments are generated by pepsin digestion to remove most of the Fc fragment, this avoids recognition by Fc receptors ...
In one approach to overcome these limitations, recombinant binding fragments (Fab, Fv or scFv) or domains (VH, VHH) of ... Skrlec, K; Strukelj, B; Berlec, A (July 2015). "Non-immunoglobulin scaffolds: a focus on their targets". Trends Biotechnol. 33 ... Crivianu-Gaita, V; Thompson, M (November 2016). "Aptamers, antibody scFv, and antibody Fab' fragments: An overview and ... This strategy is also valid for antibody fragments. However, in the absence of specific structural data, other strategies must ...
Monoclonal antibody therapy
... the Fab (fragment-antigen binding) part can be separated from the Fc (fragment constant) part of the molecule. The Fab ... Omalizumab inhibits human immunoglobulin E (IgE) and is useful in moderate-to-severe allergic asthma. Alzheimer's disease (AD) ... Jefferis R, Lefranc MP (July-August 2009). "Human immunoglobulin allotypes: possible implications for immunogenicity". mAbs. 1 ... Immunoglobulin G (IgG) antibodies are large heterodimeric molecules, approximately 150 kDa and are composed of two kinds of ...
List of MeSH codes (D12.776)
... immunoglobulin fab fragments MeSH D12.776.377.715.548.538.500 - immunoglobulin fc fragments MeSH D12.776.377.715.548.538. ... immunoglobulin fragments MeSH D12.776.377.715.548.680.650 - immunoglobulin fab fragments MeSH D12.776.377.715.548.680.650.500 ... immunoglobulin fab fragments MeSH D12.776.377.715.548.797.590 - immunoglobulin joining region MeSH D12.776.377.715.548.900 - ... immunoglobulin fc fragments MeSH D12.776.377.715.548.680.660.249 - cd4 immunoadhesins MeSH D12.776.377.715.548.680.660.500 - ...
Single chain variable fragments (scFv) and Fab fragments also bind to Protein L. Despite this wide binding range, Protein L is ... immobilize or detect immunoglobulins. Each of these immunoglobulin-binding proteins has a different antibody binding profile in ... Binding of mouse immunoglobulins is restricted to those having VκI light chains. Given these specific requirements for ... Unlike Protein A and Protein G, which bind to the Fc region of immunoglobulins (antibodies), Protein L binds antibodies through ...
"Introducing antigen-binding sites in structural loops of immunoglobulin constant domains: Fc fragments with engineered HER2/neu ... This type of antibodies are therefore able to recognise two different antigens, one at their Fab region and a second one at the ... This antibody fragment is part of the modular antibody technology of F-star Biotechnology Ltd. Wozniak-Knopp G, Bartl S, Bauer ... Fcabs are antibodies fragments engineered from the constant region of an antibody (Fc). In naturally occurring antibodies (such ...
SpAB protein domain
... and Fab (the Ig fragment responsible for antigen recognition). The native state of the B domain, deviates a lot since its inter ... Protein A contains five highly homologous immunoglobulin (Ig)-binding domains in tandem (designated domains E, D, A, B and C), ... "Crystal structure of a Staphylococcus aureus protein A domain complexed with the Fab fragment of a human IgM antibody: ... It does this though SpAB binding to the Fc fragment of IgG. The B domain of SpA (SpAB) consists of three a-helices which are ...
... and even smaller than Fab fragments (~50 kDa, one light chain and half a heavy chain) and single-chain variable fragments (~25 ... these are called VHH fragments. Cartilaginous fishes also have heavy-chain antibodies (IgNAR, 'immunoglobulin new antigen ... A single-domain antibody (sdAb), also known as a nanobody, is an antibody fragment consisting of a single monomeric variable ... An alternative approach is to split the dimeric variable domains from common immunoglobulin G (IgG) from humans or mice into ...
Fab construction is a complex technique as Fab fragments contain both variable domains and constant regions. The same heavy and ... "Dextran as a Generally Applicable Multivalent Scaffold for Improving Immunoglobulin-Binding Affinities of Peptide and ... "Therapeutic anti-IgE monoclonal antibody single chain variable fragment (scFv) safety and immunomodulatory effects after one ... light variable chains used for scFv construction can be used in the construction of Fab. Construction of pre-made phage display ...
When IgG molecules, specific for a certain antigen or surface component, bind to the pathogen with their Fab region (fragment ... Two types of FcεR are known: the high-affinity receptor FcεRI is a member of the immunoglobulin superfamily (it has two Ig-like ... Its name is derived from its binding specificity for a part of an antibody known as the Fc (fragment crystallizable) region. Fc ... It is composed of two extracellular Ig-like domains, and is a member of both the immunoglobulin superfamily and the multi-chain ...
The ability to manipulate the antibody genes make it possible to generate new antibodies and antibody fragments, such as Fab ... Affimer binders have been produced to a large number of targets including ubiquitin chains, immunoglobulins and C-reactive ... Synthetic antibodies include recombinant antibodies, nucleic acid aptamers and non-immunoglobulin protein scaffolds. As a ... or from non-immunoglobulin protein scaffolds / peptide aptamers, into which hypervariable loops are inserted to form the ...
It binds the heavy chain within the Fc region of most immunoglobulins and also within the Fab region in the case of the human ... Deisenhofer J (April 1981). "Crystallographic refinement and atomic models of a human Fc fragment and its complex with fragment ... "Crystal structure of a Staphylococcus aureus protein A domain complexed with the Fab fragment of a human IgM antibody: ... Protein A can bind with strong affinity to the Fc portion of immunoglobulin of certain species as shown in the below table. In ...
"Entrez Gene: FCAR Fc fragment of IgA, receptor for". Bakema JE, van Egmond M (November 2011). "The human immunoglobulin A Fc ... as shown by targeting FcαRI in transgenic mice models with anti-FcαRI Fab antibodies, which mimic the binding of monomeric IgA ... Fc fragment of IgA receptor (FCAR) is a human gene that codes for the transmembrane receptor FcαRI, also known as CD89 (Cluster ... Aleyd E, Heineke MH, van Egmond M (November 2015). "The era of the immunoglobulin A Fc receptor FcαRI; its function and ...
TNX-224, an anti-Factor D Fab, the antigen-binding fragment of a humanized monoclonal antibody targeting Factor D of the human ... an anti-immunoglobulin E chimeric monoclonal antibody, in patients with seasonal allergic rhinitis". Clin Pharmacol Ther. 62 (6 ... an Fab fragment of a humanized antibody against Factor D of the human immune complement system to be tested for treating ... that pertained to the use of humanized antibodies for targeting immunoglobulin E (IgE) and IgE-expressing B lymphocytes for the ...
Index of biochemistry articles
... eye proteins fab immunoglobulin - facilitated diffusion - factor VIII - FADH - FADH2 - Fat - Fatty acid - fc immunoglobulin - ... peptide fragment - peptide initiation factor - peptide receptor - peptide termination factor - peripheral membrane protein - ... immunoglobulin - immunoglobulin joining region - immunoglobulin variable region - immunologic receptor - immunology - In vivo ... heavy-chain immunoglobulin - Hela cell - helminth protein - helper T cell - hemopexin - hemoglobin - herpes simplex virus ...
Acute lymphoblastic leukemia
FAB) system that heavily relied on morphological assessment. The FAB system takes into account information on size, cytoplasm, ... This technology uses a single chain variable fragment (scFv) designed to recognize the cell surface marker CD19 as a method of ... next to the immunoglobulin heavy- or light-chain gene enhancers, leading to increased C-MYC expression and increased cell ... French-American-British (FAB) co-operative group". British Journal of Haematology. 33 (4): 451-8. doi:10.1111/j.1365-2141.1976. ...
Universal Darwinism Work by Rodney Porter with the enzyme papain resulted in cleavage of the antibody into Fab and Fc fragments ... set of whole genome duplication events at the origin of vertebrates that gave rise to the entire super-family of immunoglobulin ... and heavy-chain fragments. Together, this work allowed the antibody structure to be sequenced and reconstructed, resulting in ... structure of the vertebrate antibody by cleaving the covalent disulfide bridges that join the component chain fragments ...
Derek George Smyth
... juxtaposing the FAB and (FAB)2 fragments in the 4-chain IgG molecule, locating the bridge that links the half molecules, and ... Smyth, D.G. and Utsumi, S. (1967) Structure at the "hinge" region of rabbit immunoglobulin-G. Nature, 216, 232-235. Fanger, M.W ... Smyth, D.G., Snell, C.R. and Massey, D.E. (1978) Isolation of the C-fragment and C'-fragment of lipotropin from pig pituitary ... which they called the C-Fragment of lipotropin. … The most significant of the endorphins seems likely to be the C-Fragment. A ...
Antibodies are Y-shaped proteins produced by some B cells and are composed of two regions: an antigen-binding fragment (Fab), ... Durvalumab (Imfinzi) is a human immunoglobulin G1 kappa (IgG1κ) monoclonal antibody that blocks the interaction of programmed ... Anti-PD-1 drugs contain not only an Fab region that binds PD-1 but also an Fc region. Experimental work indicates that the Fc ... Antibodies are formed of a binding region (Fab) and the Fc region that can be detected by immune system cells via their Fc ...
An antibody digested by papain yields three fragments: two 50 kDa Fab fragments and one 50 kDa Fc fragment. The papain-digested ... In immunology, papain is known to cleave the Fc (crystallisable) portion of immunoglobulins (antibodies) from the Fab (antigen- ... the Fab fragment is still able to bind to and neutralize appropriate antigens, most commonly seen in the use of sheep anti- ... Crotalid toxin antibody preparations, known as CroFab and in Digibind, a similar sheep antiserum fragment, used to neutralize ...
Its Fab fragment was crystallized in complex with the ED3 domain of DENV2. Its epitope straddles β-strands A and G of ED3. The ... ED3 is a continuous polypeptide segment; its fold is compact and immunoglobulin-like. Dengue virus is transmitted by species of ... Its Fab fragment was crystallized in complex with the sE protein of DENV4. Its epitope is included in domain 1 (ED1) of the E ... Its Fab and scFv fragments were crystallized in complex with the ED3 domain of DENV1. Its epitope is located around β-strands C ...
A key feature of these models included the idea that the antigen binding domains of antibodies (Fab) include amino acids from ... Edelman and his colleagues used cyanogen bromide and proteases to fragment the antibody protein subunits into smaller pieces ... Cummingham, B.; Gottlieb, P.; Konigsberg, W.; Edelman, G. (1968). "The covalent structure of a human gamma G-immunoglobulin. V ... "The covalent structure of an entire gammaG immunoglobulin molecule". Proceedings of the National Academy of Sciences of the ...
Immunoglobulin G (IgG) is the major type of antibody present in the serum. It is part of the adaptive immune system, but it ... The antibody binds to microbes with the variable Fab domain, and the Fc domain binds to Fc receptors (FcR) to induce ... They need to retain protein fragments of a suitable size for specific bacterial recognition, so the peptides are only partially ...
Different mechanisms of antibody-mediated neutralization of parvoviruses revealed using the Fab fragments of monoclonal...
Eight monoclonal antibodies and their Fab fragments were tested for neutralization of canine parvovirus and feline ... Immunoglobulin Fab Fragments / immunology* * Immunoglobulin G / immunology * Leukemia Virus, Feline / immunology* * ... All Fabs reduced capsid binding of virus to purified feline TfR in vitro, but the highly neutralizing Fabs were more efficient ... Eight monoclonal antibodies and their Fab fragments were tested for neutralization of canine parvovirus and feline ...
Competent antigen-binding fragments (Fab) from secretory immunoglobulin a using streptococcus sanguis immunoglobulin a protease...
Competent antigen-binding fragments (Fab) from secretory immunoglobulin a using streptococcus sanguis immunoglobulin a protease ... Competent antigen-binding fragments (Fab) from secretory immunoglobulin a using streptococcus sanguis immunoglobulin a protease ... Competent antigen-binding fragments (Fab) from secretory immunoglobulin a using streptococcus sanguis immunoglobulin a protease ... T1 - Competent antigen-binding fragments (Fab) from secretory immunoglobulin a using streptococcus sanguis immunoglobulin a ...
Abciximab (ReoPro) use while Breastfeeding
JCI - Production of Antibodies Specific for Fc, Fab′, and Streptokinase-Streptodornase In Vitro by Peripheral Blood Cells from...
FRAGMENTS OF IMMUNOGLOBULIN G. ... the immunoglobulin (Ig)M anti-Fc, anti-Fab′, and ... IDENTIFICATION OF IMMUNE COMPLEXES IN CULTURE SUPERNATANTS CONTAINING HIDDEN ANTIBODIES REACTIVE WITH Fab′ FRAGMENTS OF ... Production of Antibodies Specific for Fc, Fab′, and Streptokinase-Streptodornase In Vitro by Peripheral Blood Cells from ... Production of Antibodies Specific for Fc, Fab′, and Streptokinase-Streptodornase In Vitro by Peripheral Blood Cells from ...
Third-Degree Atrioventricular Block (Complete Heart Block) Medication: Sympathomimetic agents or vagolytic agents,...
SCOPe 2.08: Structural Classification of Proteins - extended
Description: igg-fab fragment of mouse monoclonal antibody ctm01. Class: immunoglobulin. Keywords: immunoglobulin, fab fragment ... Compound: igg ctm01 fab (heavy chain). Species: Mus musculus [TaxId:10090]. Database cross-references and differences (RAF- ... Compound: igg ctm01 fab (light chain). Species: Mus musculus [TaxId:10090]. Database cross-references and differences (RAF- ...
Investigational Heptavalent Botulinum Antitoxin (HBAT) to Replace Licensed Botulinum Antitoxin AB and Investigational Botulinum...
... intact immunoglobulin G (IgG) and ≥90% Fab and F(ab)2 immunoglobulin fragments; these fragments are created by the enzymatic ... Fab and F(ab)2 fragments are cleared from circulation more rapidly than intact IgG (2), and repeat HBAT dosing might be ... cleavage and removal of Fc immunoglobulin components in a process sometimes referred to as despeciation. ...
protein data bank
Fab Fragments Fab Fragments, Immunoglobulin Fab Immunoglobulin Fragments Fragment Immunoglobulins, Fab Fragment, Fab Ig Fab ... Fab Fragment. Fab Fragment Immunoglobulins. Fab Fragment, Immunoglobulin. Fab Fragments. Fab Fragments, Immunoglobulin. Fab ... Fragment Immunoglobulins, Fab. Fragment, Fab. Ig Fab Fragments. Immunoglobulin Fab Fragment. Immunoglobulin Fragments, Fab. ... Immunoglobulin Fab Fragments Entry term(s). Fab Fragment Fab Fragment Immunoglobulins Fab Fragment, Immunoglobulin ...
sg:pub.10.1186/s12968-016-0299-1 - Springer Nature SciGraph
GPIIb-IIIa antagonist-induced reduction in platelet surface factor V/Va binding and phosphatidylserine expression in whole blood
Improved tumor targeting with chemically cross-linked recombinant antibody fragments. - Wellcome Centre for Human Genetics
To facilitate cross-linking of Fab fragments, a chimeric B72.3 Fab fragment has been expressed with a hinge sequence ... Fragments such as F(ab)2, Fab, Fv and single chain Fv (scFv) offer faster blood clearance but also lower overall tumor doses ... These fragments, Fab delta Cys and scFv delta Cys were cross-linked with linkers containing two or three maleimide groups to ... cB72.3 90Y-labeled tri-Fab was found not to accumulate in the kidney or the bone, resulting in an attractive antibody fragment ...
Elliot Frohman - Research output - University of Texas Southwestern Medical Center
PRIME PubMed | Comparison of Preclinical Properties of Several Available Antivenoms in the Search for Effective Treatment of...
AnimalsAntibodies, NeutralizingAntibody SpecificityAntiveninsEuropeHealth ResourcesImmunoglobulin Fab FragmentsSnake BitesTime ... Intravenous Vipera berus Venom-Specific Fab Fragments and Intramuscular Vipera ammodytes Venom-Specific F(ab)2 Fragments in ... ViperaTAb contains Fab fragments against the venom of V. berus. In 2014 the production of Zagreb antivenom was discontinued. ... ViperaTAb contains Fab fragments against the venom of V. berus. In 2014 the production of Zagreb antivenom was discontinued. ...
Human Immunoglobulin G (IgG-Fab) Antibody | 24133-05011 | Universal Biologicals
Human Immunoglobulin G Fab fragment purified from human serum Antibody Type. Polyclonal ... immunoglobulin, IGHG 1, IGHG 2, IGHG 3, IGHG 4, Immunoglobulin Gm3, Immunoglobulin Gm4, Immunoglobulin Gm2, Immunoglobulin Gm1 ... Human Immunoglobulin G (IgG-Fab) Antibody. Move your mouse over image or click to enlarge ...
DeCS 2018 - July 31, 2018 version
Fab Fragment Immunoglobulins use Immunoglobulin Fab Fragments Fab Fragment, Immunoglobulin use Immunoglobulin Fab Fragments ... Fab Fragments, Immunoglobulin use Immunoglobulin Fab Fragments Fab Immunoglobulin Fragments use Immunoglobulin Fab Fragments ... Fab Fragments use Immunoglobulin Fab Fragments ...
Naoki Kaneko, Ph.D., D.D.S. | Harvard Catalyst Profiles | Harvard Catalyst
Third-Degree Atrioventricular Block (Complete Heart Block) Medication: Sympathomimetic agents or vagolytic agents,...
Presumed Copperhead Snakebite and Antivenom Administration in the Third Trimester. | [email protected]
Immunoglobulin Fab Fragments * Pregnancy * Pregnancy Outcome * Snake Bites Attention Stats. *About. *Support ... Crotalidae polyvalent immune Fab was administered. The patient felt significantly better with improvement in swelling. She had ... This case supports the use of Crotalidae polyvalent immune Fab for venomous snakebites in pregnant patients to prevent possible ...
Human IgA Immunoglobulin Monoclonal Mouse Antibody (IA761) - Biotium
The chains form two domains, the Fab (antigen binding) fragment and the Fc (constant) fragment. Immunoglobulin A (IgA) is the ... Immunoglobulin Am2; Immunoglobulin Heavy Constant Alpha 1; Immunoglobulin Heavy Constant Alpha 2 ... Immunoglobulins are four-chain, Y-shaped, monomeric structures comprised of two identical heavy chains and two identical light ... This MAb is specific to heavy chain of IgA and shows minimal cross-reaction with heavy chains of other immunoglobulins. It is ...
Anti-Phencyclidine Monoclonal Antibody - InterveXion Therapeutics
Disposition of a monoclonal anti-phencyclidine Fab fragment of immunoglobulin G in rats. J Pharmacol Exp Ther. 1993 Sep;266(3): ... Antiphencyclidine monoclonal Fab fragments reverse phencyclidine-induced behavioral effects and ataxia in rats. J Pharmacol Exp ... Anti-phencyclidine monoclonal Fab fragments markedly alter phencyclidine pharmacokinetics in rats. J Pharmacol Exp Ther. 1994 ... Crystal structure of monoclonal 6B5 Fab complexed with phencyclidine. J Biol Chem. 1998 Oct 30;273(44):28576-82. PubMed PMID: ...
Antibody - Wikipedia
"Primary structure of a human IgA1 immunoglobulin. IV. Streptococcal IgA1 protease, digestion, Fab and Fc fragments, and the ... Antibody fragments, such as Fab and nanobodies are not considered as antibody mimetics. Common advantages over antibodies are ... Structurally an antibody is also partitioned into two antigen-binding fragments (Fab), containing one VL, VH, CL, and CH1 ... Somatic recombination of immunoglobulins, also known as V(D)J recombination, involves the generation of a unique immunoglobulin ...
Leukocyte-platelet aggregation, platelet surface P-selectin, and platelet surface glycoprotein IIIa after percutaneous coronary...
Enhancement of colorectal tumor targeting using a novel biparatopic monoclonal antibody against carcinoembryonic antigen in...
Localization indices of the tumor in various organs revealed 1.3 to 4.1 in PR1A3 IgG-pretreated mice, 2.4 to 6.6 in fragment ... Tumors were localized using 125I-labeled MAbs: IgG, F(ab)(2) and Fab of PR1A3, and biparatopic MAb of PR1A3 and T84.66. ... fragments from PR1A3 and T84.66. Fifty-nine tumors from 2 human colorectal carcinoma cell lines with high (KM-12c) and low ( ... Biparatopic MAb was prepared by using cross-linking of reduced Fab ...
Pesquisa | Portal Regional da BVS
We found that the use of Fab fragments or immunoglobulins from different species or reversal of the antibody pair led to either ... Also, the use of higher plasma dilutions or pre-clearing of plasma immunoglobulins were ineffective. Finally, we found that a ... Fragmentos Fab das Imunoglobulinas/imunologia , Imunoglobulina G/imunologia , Camundongos , Ativação de Neutrófilo , ...
Structures of the sugar chains of rabbit immunoglobulin G: occurrence of asparagine-linked sugar chains in Fab fragment.. 1985 ... Structures of asparagine linked oligosaccharides of immunoglobulins (IgY) isolated from egg-yolk of Japanese quail.. 1993. ... Comparative structural study of the N-linked oligosaccharides of human normal and pathological immunoglobulin G.. 1987. ...
Protein Concepts Dictionary
IMMUNOGLOBULIN FAB FRAGMENTS 2.3 IMMUNOGLOBULIN DOMAIN 2.3 IMMUNOGLOBULIN 2.3 IMMUNE RESPONSE 2.3 HUMAN PODOPLANIN 2.3 FAB 2.3 ... IMMUNOGLOBULIN LIKE 6.2 HLA A2 6.2 5 OP RU 5.4 PROTEIN BINDING 5.4 MHC FOLD 5.4 IG FOLD 5.4 GLYCOLIPID ANTIGEN PRESENTATION 5.4 ... IMMUNOGLOBULIN FOLD 3.9 GLYCOPROTEIN 3.9 CROSS REACTIVITY 3.9 AUTOIMMUNITY 3.1 VIRULENCE FACTORS 3.1 TCR DMF5 3.1 TCR DMF4 3.1 ...
AntibodiesSpecificityProteinsDisulfide bondsAntibody fragmentSerumPEGylated anti-TNFSpeciesCrotalidae polyvalent immune FabAffinityAntigen-bindingProteolyticProteaseRheumatoid arthritisChainsIgG1Overlap extensioMoleculesDigestionCleavageAntivenomPolyspecificRecombinantProteinTumor1993VitroMolecular weightHumanHingeFoldLightSpecificChainRapidlyStructure
- Eight monoclonal antibodies and their Fab fragments were tested for neutralization of canine parvovirus and feline panleukopenia virus. (nih.gov)
- Antibodies are glycoproteins belonging to the immunoglobulin superfamily . (wikipedia.org)
- Fab fragment antibodies are generated by papain digestion of whole IgG antibodies to remove the entire Fc portion, including the hinge region. (jacksonimmuno.com)
- Therapeutic monoclonal antibodies and Fc-fusion proteins containing antibody Fc fragment may tend to destabilize (e.g. unfold and aggregate), which leads to loss of functions and increase of adverse risks. (omicsdi.org)
- Bispecific antibodies such as the BPCAM antibody are among the next-generation antibody therapeutics capable of binding two separate target antigens using the two components of immunoglobulin G (IgG) molecules. (lifestylesimplify.com)
- Out of the existing antibodies, three Fab therapeutic fragments have been approved by FDA. (lifestylesimplify.com)
- These three fragments of therapeutic antibodies are ReoPro® (abciximab) - an anti-gpIIb/IIIa Fab fragment meant to prevent blood clots in angioplasty. (lifestylesimplify.com)
- An improvement in the development of antibodies expansion in structure and functions of immunoglobulin G gave space for the next generation antibodies fragments, which are in clinical testing. (lifestylesimplify.com)
- When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition. (rockland.com)
- 1990). The specificity of immunoglobulins depends upon the amino acidity sequences of three hypervariable loops of both heavy as well as the light chains of the variable site. (iassist2012.org)
- 1 One neutralizing unit is determined as the amount of the mixed monospecific Fab proteins necessary to neutralize one LD 50 of each of the four venoms, where the LD 50 is the amount of venom that would be lethal in 50% of mice. (druginserts.com)
- The circulating monocytes are found to be deregulated in ALS regarding subtype constitution, function and gene expression and application of human immunoglobulins or fusion proteins containing only the human Fc, but not the Fab antibody fragment increased CNS invasion of peripheral monocytes and delayed the disease onset. (semanticscholar.org)
- No antibody was detected against non-immunoglobulin serum proteins. (jacksonimmuno.com)
- For this reason a ribosomal skipping sequence enabling the translation of two separate proteins from one open reading frame was genetically incorporated between the displayed antibody fragment and a green fluorescent protein (GFP) as reporter protein. (tu-darmstadt.de)
- Univalent antigen-binding fragments composed of one entire IMMUNOGLOBULIN LIGHT CHAIN and the amino terminal end of one of the IMMUNOGLOBULIN HEAVY CHAINS from the hinge region, linked to each other by disulfide bonds. (bvsalud.org)
- Formation of disulfide bonds between heavy and light chains is challenge for production of recombinant immunoglobulin G (IgG) and fragment antigen-binding (Fab). (elsevier.com)
- Stabilisation of the Fc fragment of human IgG1 by engineered intradomain disulfide bonds. (omicsdi.org)
- We report the stabilization of the human IgG1 Fc fragment by engineered intradomain disulfide bonds. (omicsdi.org)
- Additionally, we have introduced the intradomain disulfide bonds into an IgG Fc fragment engineered in C-terminal loops of the CH3 domain for binding to Her2/neu, and observed an increase of the T(m) of the CH3 domain for 7.5°C for CysP4, 15.5°C for CysP2 and 19°C for the CysP2 and CysP4 disulfide bonds combined in one molecule. (omicsdi.org)
- Comprehensive elucidation of the structural and functional roles of engineered disulfide bonds in antibody Fc fragment. (omicsdi.org)
- Our results give a comprehensive elucidation of structural and functional effects caused by additional disulfide bonds in the Fc fragment, which is important for Fc engineering toward the desired clinical performance. (omicsdi.org)
- The interchain disulfide bonds are preserved, the two Fab' fragments remain associated in a form called F(ab') 2 . (imgt.org)
- cB72.3 90Y-labeled tri-Fab was found not to accumulate in the kidney or the bone, resulting in an attractive antibody fragment for tumor therapy. (ox.ac.uk)
- Translation of the protein of interest and the ribosomal skipping sequence (called 2A peptide) results in release of the N-terminally located antibody fragment from the polypeptide chain and secretion to the cell surface. (tu-darmstadt.de)
- Each monospecific antivenin is prepared by fractionating the immunoglobulin from the ovine serum, digesting it with papain, and isolating the venom specific Fab fragments on ion exchange and affinity chromatography columns. (druginserts.com)
- Immunoglobulins: Types and Functions and the complement system Complement system Serum glycoproteins participating in the host defense mechanism of complement activation that creates the complement membrane attack complex. (lecturio.com)
- IgG IgG The major immunoglobulin isotype class in normal human serum. (lecturio.com)
- Cimzia® (certolizumab pegol) - this antibody is a PEGylated anti-TNFα Fab fragment used for Crohn's disease and rheumatoid arthritis. (lifestylesimplify.com)
- We have examined the tumor targeting of several novel fragments produced by chemical cross-linking of Fab' or scFv to dimeric and trimeric species. (ox.ac.uk)
- 1. To be eligible for risk-benefit assessment by WHO, an antivenom product must consist of a polyspecific antivenom immunoglobulin preparation, with claimed efficacy in treating envenoming by Bungarus caeruleus , Daboia russelii , Echis carinatus and Naja naja (sometimes referred to as the "big four" species)1. (who.int)
- The antibody may cross-react with immunoglobulins from other species. (jacksonimmuno.com)
Crotalidae polyvalent immune Fab3
- Crotalidae polyvalent immune Fab was administered. (duke.edu)
- This case supports the use of Crotalidae polyvalent immune Fab for venomous snakebites in pregnant patients to prevent possible maternal and fetal morbidity and mortality. (duke.edu)
- CROFAB [Crotalidae Polyvalent Immune Fab (Ovine)] is a sterile, nonpyrogenic, purified, lyophilized preparation of ovine Fab (monovalent) immunoglobulin fragments obtained from the blood of healthy sheep flocks immunized with one of the following North American snake venoms: Crotalus atrox (Western Diamondback rattlesnake), Crotalus adamanteus (Eastern Diamondback rattlesnake), Crotalus scutulatus (Mojave rattlesnake), and Agkistrodon piscivorus (Cottonmouth or Water Moccasin). (druginserts.com)
- There was no specific correlation between Fab binding affinity and neutralization. (nih.gov)
- Digoxin immune Fab is an immunoglobulin fragment with a specific and high affinity for both digoxin and digitoxin molecules. (medscape.com)
- Pharmacokinetic mechanisms for obtaining high renal coelimination of phencyclidine and a monoclonal antiphencyclidine antigen-binding fragment of immunoglobulin G in the rat. (intervexion.com)
- 11. The method of any one of claims 1 to 10, wherein the anti-ILT4 antibody or antigen binding fragment thereof comprises a heavy chain variable region of SEQ ID NO: 19 and a light chain variable region of SEQ ID NO: 14. (sumobrain.com)
- 15. The method of claim 13 or 14, wherein the PD-1 antagonist is an anti-PD-1 antibody or antigen binding fragment thereof. (sumobrain.com)
- 18. The method of claim 15, wherein the anti-PD-1 antibody or antigen binding fragment thereof comprises a heavy chain variable region of SEQ ID NO:9 and a light chain variable region of SEQ ID NON. (sumobrain.com)
- 19. The method of claim 15, wherein the anti-PD-1 antibody or antigen binding fragment thereof comprises a heavy chain of SEQ ID NO: 10 and a light chain of SEQ ID NO:5. (sumobrain.com)
- This fragment can be obtained by digestion of immunoglobulins with the proteolytic enzyme PAPAIN . (bvsalud.org)
- The Fab fragments are the simplest form of antibody- p53 antibody , a derived molecule formed by proteolytic digestion. (lifestylesimplify.com)
- Rat IgG F(ab')2 Fragment Peroxidase Conjugated is a proteolytic fragment of immunoglobulin G (IgG) obtained by limited digestion with the enzyme pepsin under controlled conditions of temperature, time and pH. (rockland.com)
- Immunoglobulin A (IgA) protease, a family of bacterial enzymes, cleaves human IgA I at a single bond generating distinct Fab and Fc fragments. (nyu.edu)
- Deleted genes encoded the glycerol transport and metabolism systems GtsABCD and GlpOKF and the Mycoplasma Ig binding protein-Mycoplasma Ig protease (MIB-MIP) immunoglobulin cleavage system. (bvsalud.org)
- To study antibody (Ab) biosynthesis in rheumatoid arthritis (RA), the immunoglobulin (Ig)M anti-Fc, anti-Fab′, and antistreptokinase-streptodornase (SKSD) produced by peripheral blood lymphocytes (PBL) were measured at intervals from 1 to 19 d in culture. (jci.org)
- Structures of the sugar chains of rabbit immunoglobulin G: occurrence of asparagine-linked sugar chains in Fab fragment. (unicarbkb.org)
- It also reacts with the light chains of other rat immunoglobulins. (jacksonimmuno.com)
- IgM IgM A class of immunoglobulin bearing mu chains (immunoglobulin mu-chains). (lecturio.com)
- Solitary crystal SU1498 X-ray diffraction research show that antibody Fab fragments are multimeric protein comprising light (L) and weighty (H) polypeptide chains showing up as four homologous globular domains, structured SU1498 in pairs, that talk about a common 3-D set up. (iassist2012.org)
- Furthermore, reactivity analysis using an enzyme-linked immunosorbent assay (ELISA) revealed that anti-PT Fab IgG1 subtype showed the highest reactivity against target compound paclitaxel. (elsevier.com)
- To investigate how amino acid sequences of the first heavy chain constant region (CH1) effect on assembly between heavy chain (VH-CH1 or Fd) and light chain (VL-CL), the CH1 gene sequence of an anti-paclitaxel fragment antigen-binding (anti-PT Fab IgG2a) was modified using the splicing by overlap extension-polymerase chain reaction (SOE-PCR) to be gene sequences encoding amino acid sequence of IgG1, IgG2b, and IgG3 subtypes. (elsevier.com)
- To this end, yeast-displayed recombinant antibody libraries from splenic mRNA of chickens immunized with epidermal growth factor receptor (EGFR) and human chorionic gonadotropin (hCG) were constructed as single chain variable fragments (scFv) by overlap extension polymerase chain reaction. (tu-darmstadt.de)
- These fragments, Fab' delta Cys and scFv' delta Cys were cross-linked with linkers containing two or three maleimide groups to produce dimeric and trimeric molecules with increased avidity for antigen. (ox.ac.uk)
- Fc fragments and whole IgG molecules have been removed. (jacksonimmuno.com)
- This product possesses the F(ab')2 fragment, recognized by the two F(ab) fragments yielded from the digestion of the antibody below the disulfide bond hinge region followed by Peroxidase conjugation. (rockland.com)
- these fragments are created by the enzymatic cleavage and removal of Fc immunoglobulin components in a process sometimes referred to as despeciation. (cdc.gov)
- Zagreb antivenom comprises V. ammodytes-specific F(ab')2 fragments. (unboundmedicine.com)
- The World Health Organization (WHO), acting through its Regulation and Prequalification Department, is now calling for applications from licensed manufacturers of snake antivenom immunoglobulin products who wish to have those products evaluated for potential listing by WHO as recommended for procurement. (who.int)
- Viperfav is a polyspecific preparation based on F(ab')2 fragments against V. aspis, V. berus, and V. ammodytes venoms. (unboundmedicine.com)
- Improved tumor targeting with chemically cross-linked recombinant antibody fragments. (ox.ac.uk)
- Insufficient formation of the bond influences productive yield and quality of recombinant IgG and Fab. (elsevier.com)
- The folding efficiency and reactivity of the anti-PT Fab was influenced by CH1 amino acid sequence, which raises the possibility that this modification can be applied to improve recombinant Fab production. (elsevier.com)
- An antibody ( Ab ), also known as an immunoglobulin ( Ig ),  is a large, Y-shaped protein used by the immune system to identify and neutralize foreign objects such as pathogenic bacteria and viruses . (wikipedia.org)
- The corresponding PDB (Protein Data Bank) format file is from Mike's immunoglobulin structure function site (http://www.umass.edu/microbio/rasmol/padlan.htm) . (imgt.org)
- Fragments such as F(ab')2, Fab', Fv and single chain Fv (scFv) offer faster blood clearance but also lower overall tumor doses. (ox.ac.uk)
- Biodistribution studies in the nude mouse LS174T xenograft model with scFv, di-scFv, and tri-scFv demonstrated that these fragments clear extremely rapidly from the circulation and give rise to only low levels of activity accumulated at the tumor. (ox.ac.uk)
- Di-Fab (DFM) and tri-Fab (TFM) however, accumulated relatively high levels of activity at the tumor with high tumor:blood ratios generated, demonstrating improved targeting compared to IgG. (ox.ac.uk)
- Localization indices of the tumor in various organs revealed 1.3 to 4.1 in PR1A3 IgG-pretreated mice, 2.4 to 6.6 in fragment MAbs of PR1A3-pretreated mice and 2 to 4.6 in biparatopic MAb-pretreated mice. (ox.ac.uk)
- Structural research of idiotypic cascades have already been completed using specifically antibody fragments (evaluated in Mariuzza and Poljak, 1993, Skillet et al. (iassist2012.org)
- All Fabs reduced capsid binding of virus to purified feline TfR in vitro, but the highly neutralizing Fabs were more efficient competitors. (nih.gov)
- The molecular weight of Fab fragments is about 50 kDa. (jacksonimmuno.com)
- Comparative structural study of the N-linked oligosaccharides of human normal and pathological immunoglobulin G. (unicarbkb.org)
- Here, we describe HIV-1 Env Fab-dimerized glycan (FDG)-reactive bnAbs without VH-swapped domains from simian-human immunodeficiency virus (SHIV)-infected macaques. (bvsalud.org)
- To facilitate cross-linking of Fab' fragments, a chimeric B72.3 Fab' fragment has been expressed with a hinge sequence containing a single cysteine residue. (ox.ac.uk)
- The immunoglobulin fold includes two antiparallel -bedding shaped by three and four antiparallel strands in the continuous light (CL) and weighty (CH1) domains, and five and four antiparallel strands in adjustable light (VL) and weighty (VH) domains. (iassist2012.org)
- The conservative method for Fab library generation relies on using a three-step protocol including individual heavy- and light chain sub-libraries generated in haploid yeast strains followed by chain combination using yeast mating. (tu-darmstadt.de)
- Each vial contains 40 mg of purified digoxin-specific antibody fragments, which will bind approximately 0.6 mg of digoxin or digitoxin. (medscape.com)
- CROFAB is a venom-specific Fab fragment of immunoglobulin G (IgG) that works by binding and neutralizing venom toxins, facilitating their redistribution away from target tissues and their elimination from the body. (druginserts.com)
- 2G12 is a broadly neutralizing Ab (bnAb) that targets a conserved glycan patch on Env of geographically diverse HIV-1 strains using a unique heavy-chain (VH) domain-swapped architecture that results in fragment antigen-binding (Fab) dimerization. (bvsalud.org)
- Fab and F(ab') 2 fragments are cleared from circulation more rapidly than intact IgG ( 2 ), and repeat HBAT dosing might be indicated for some wound or intestinal colonization patients if in situ botulinum toxin production continues after clearance of antitoxin. (cdc.gov)
- Crystal structure of monoclonal 6B5 Fab complexed with phencyclidine. (intervexion.com)