Immunologic techniques based on the use of: (1) enzyme-antibody conjugates; (2) enzyme-antigen conjugates; (3) antienzyme antibody followed by its homologous enzyme; or (4) enzyme-antienzyme complexes. These are used histologically for visualizing or labeling tissue specimens.

Immunohistochemical expression of mdm2 and p21WAF1 in invasive cervical cancer: correlation with p53 protein and high risk HPV infection. (1/12862)

AIM: To investigate the immunocytochemical staining pattern of mdm2 and p21WAF1 proteins in invasive cervical cancer and to determine its relation with the expression of p53 and with the high risk HPV infection. METHODS: Immunocytochemistry for p53, mdm2, and p21WAF1 was performed in 31 paraffin embedded sections of invasive cervical cancer. The results were assessed by image analysis, evaluating for each protein the optical density of the immunostained area, scored as percentage of the total nuclear area. The presence of high risk human papillomavirus (HPV) infection was detected by using the polymerase chain reaction. RESULTS: Immunostaining for both mdm2 and p21WAF1 was correlated with p53 expression; however, the correlation between p53 and mdm2 (R = 0.49; p < 0.01) was more significant than between p53 and p21WAF1 (R = 0.31; p < 0.05); the less stringent correlation between p53 and p21WAF1 might reflect the p53 independent mechanisms of p21WAF1 induction. Similar average levels of p53, mdm2, and p21WAF1 immunostaining were found in the presence or absence of high risk HPV-DNA, without significant differences between the two groups. CONCLUSIONS: These data suggest that mdm2 and p21WAF1 proteins are expressed in invasive cervical cancer and that their immunocytochemical staining pattern is not abrogated by the presence of high risk HPV genomic sequences.  (+info)

Expression of vascular endothelial growth factor in human oral squamous cell carcinoma: its association with tumour progression and p53 gene status. (2/12862)

AIMS: To correlate vascular endothelial growth factor (VEGF) expression in oral squamous cell carcinoma with the clinicopathological characteristics and prognosis; and to assess whether p53 gene status is associated with VEGF expression in human cancers. METHODS: Tumour specimens from 45 patients with oral squamous cell carcinomas were examined. Expression of VEGF was determined using an immunohistochemical method, and a tumour was considered positive when more than 5% of the neoplastic cells showed VEGF immunoreactivity. The p53 gene status was screened using a polymerase chain reaction--single strand conformation polymorphism analysis. RESULTS: VEGF positive staining was detected in 19 (42.2%) of the 45 cases. VEGF immunoreactivity did not correlate with the histological degree of tumour differentiation, clinical stages, or lymph node metastasis. The patients with VEGF positive tumours had a significantly worse prognosis than those with VEGF negative tumours. The five year overall survival rate of the VEGF negative patients was 76.5%, as compared with 48.8% for the VEGF positive patients. No significant association between VEGF expression and the p53 gene status of the tumours was found. CONCLUSIONS: VEGF is a good prognostic indicator of the survival of patients with oral squamous cell carcinoma. The p53 gene status does not seem to be associated with VEGF expression in these cancers.  (+info)

Overexpression of human homologs of the bacterial DnaJ chaperone in the synovial tissue of patients with rheumatoid arthritis. (3/12862)

OBJECTIVE: To study the expression of the chaperone family of J proteins in the synovial tissue of patients with rheumatoid arthritis (RA) or osteoarthritis. METHODS: Rabbit antibodies specific for a synthetic peptide (pHSJ1: EAYEVLSDKHKREIYD), representing the most conserved part of all J domains thus far identified--among them the Drosophila tumor suppressor Tid56--were used in immunohistochemical analyses of frozen sections of synovial tissue and immunoblotting of protein extracts of adherent synovial cells. IgG specific for Tid56 was also used. RESULTS: Both antisera predominantly and intensely stained synovial lining cells from RA patients; other cells did not stain or stained only faintly. In immunoblots, anti-pHSJ1 specifically detected several bands with molecular weights of >74 kd (type I), 57-64 kd (type II), 41-48 kd (type III), and < or =36 kd (type IV). The strongest band detected in RA adherent synovial cells was the type II band, whereas in a B cell line, a type I band was prominent. CONCLUSION: Several potentially new members of the J family are described. The type II band represents the human homolog of the Drosophila Tid56 protein and is strongly expressed in RA synovial tissue.  (+info)

Expression of B7 costimulatory molecules by salivary gland epithelial cells in patients with Sjogren's syndrome. (4/12862)

OBJECTIVE: To investigate the expression of B7 costimulatory molecules in the lymphoepithelial lesions of salivary gland (SG) biopsy tissues and in SG epithelial cell lines derived from patients with Sjogren's syndrome (SS). METHODS: B7.1 and B7.2 protein expression was studied by immunohistochemistry in minor SGs obtained from 11 patients with SS and 10 disease control patients with nonspecific sialadenitis and in cultured SG epithelial cell lines obtained from minor SGs from 15 SS patients and 15 control patients. B7.1 and B7.2 messenger RNA (mRNA) expression by SG epithelial cell lines was examined by reverse transcription-polymerase chain reaction (RT-PCR). RESULTS: In biopsy tissues from SS patients, but not control patients, ductal and acinar epithelial cells showed increased expression of both B7.1 and B7.2. Intense spontaneous B7.1 protein expression (as well as HLA-ABC, but not B7.2 or HLA-DR) was also found in 73% of SG epithelial cell lines from SS patients versus 13% of those from control patients (P < 0.01). Interferon-y treatment induced, or up-regulated, B7.1, B7.2, and HLA-DR expression in all SG epithelial cell lines tested. B7.1 and B7.2 expression by SG epithelial cell lines was also verified at the mRNA level by RT-PCR. CONCLUSION: Human SG epithelia are intrinsically capable of expressing B7 proteins upon activation. In SS patients, the expression of B7 molecules by SG epithelial tissues and by SG epithelial cell lines indicates the activated status of SG epithelial cells in this disorder and, possibly, their capacity for presenting antigens to T cells.  (+info)

Localization of sodium bicarbonate cotransporter (NBC) protein and messenger ribonucleic acid in rat epididymis. (5/12862)

An acidic environment is important for sperm maturation in the epididymis and also helps to maintain mature sperm in an immotile state during storage in this organ. Both an Na+/H+ exchanger and an H+ATPase have been implicated in this process. The H+ATPase is concentrated in specialized apical (and/or narrow) and clear cells of the epididymis, while the Na+/H+ exchanger has not yet been localized in situ. As in other proton-secreting epithelia, bicarbonate transport occurs in the epididymis, where it is implicated in luminal acidification. In this study we used an antibody raised against a fusion protein (maltose-binding protein: MBP-NBC-5) from the C-terminus of the recently cloned rat kidney Na+/HCO3- cotransporter (NBC) to localize this protein in the epididymis and vas deferens of the rat. The distribution of the respective mRNA was mapped by in situ hybridization. NBC message was strongly expressed in the initial segment and the intermediate zone of the epididymis, and the NBC-5 antibody gave a strong basolateral staining in both principal cells and apical/narrow cells in this region. Western blotting revealed a single band at about 160 kDa in the epididymis. The intensity of staining as well as mRNA levels decreased in the cauda epididymidis and in the vas deferens, where only weak staining was seen. Basolateral NBC may function in parallel with apical proton secretion to regulate luminal acidification and/or bicarbonate reabsorption in the excurrent duct system.  (+info)

Epithelial hyperproliferation and transglutaminase 1 gene expression in Stevens-Johnson syndrome conjunctiva. (6/12862)

In Stevens-Johnson syndrome, pathological keratinization of the ordinarily nonkeratinized corneal and conjunctival mucosal epithelia results in severe visual loss. We examined conjunctiva covering cornea in five eyes in the chronic cicatricial phase of Stevens-Johnson syndrome. Normal conjunctiva from five age-matched individuals was studied also. The number of epithelial cells in Stevens-Johnson syndrome conjunctiva that were immunoreactive with a monoclonal antibody, Ki-67, to a nuclear antigen found only in proliferating cells was greater than normal (93.8+/-19.8 cells above 100 basal cells versus 12.8+/-0.5 cells above 100 basal cells; P = 0.009). In addition, although clinical inflammation was mild, massive lymphocytic infiltration was seen in the substantia propria of conjunctiva covering cornea. In situ hybridization documented transglutaminase 1 (keratinocyte transglutaminase) mRNA in suprabasal cells of the abnormally thickened conjunctival epithelium in all Stevens-Johnson syndrome patients. In contrast, no message was detected in normal conjunctival or corneal epithelia. Transglutaminase 1 is expressed during the terminal differentiation of keratinocytes where it helps synthesize cornified cell envelopes. We speculate that in Stevens-Johnson syndrome, epithelial hyperproliferation, and transglutaminase 1 gene expression lead to the pathological keratinization of ocular surface mucosal epithelia.  (+info)

Rescue of diabetes-related impairment of angiogenesis by intramuscular gene therapy with adeno-VEGF. (7/12862)

Diabetes is a major risk factor for coronary and peripheral artery diseases. Although diabetic patients often present with advanced forms of these diseases, it is not known whether the compensatory mechanisms to vascular ischemia are affected in this condition. Accordingly, we sought to determine whether diabetes could: 1) impair the development of new collateral vessel formation in response to tissue ischemia and 2) inhibit cytokine-induced therapeutic neovascularization. Hindlimb ischemia was created by femoral artery ligation in nonobese diabetic mice (NOD mice, n = 20) and in control C57 mice (n = 20). Hindlimb perfusion was evaluated by serial laser Doppler studies after the surgery. In NOD mice, measurement of the Doppler flow ratio between the ischemic and the normal limb indicated that restoration of perfusion in the ischemic hindlimb was significantly impaired. At day 14 after surgery, Doppler flow ratio in the NOD mice was 0.49+/-0.04 versus 0.73+/-0.06 for the C57 mice (P< or =0.005). This impairment in blood flow recovery persisted throughout the duration of the study with Doppler flow ratio values at day 35 of 0.50+/-0.05 versus 0.90+/-0.07 in the NOD and C57 mice, respectively (P< or =0.001). CD31 immunostaining confirmed the laser Doppler data by showing a significant reduction in capillary density in the NOD mice at 35 days after surgery (302+/-4 capillaries/mm2 versus 782+/-78 in C57 mice (P< or =0.005). The reduction in neovascularization in the NOD mice was the result of a lower level of vascular endothelial growth factor (VEGF) in the ischemic tissues, as assessed by Northern blot, Western blot and immunohistochemistry. The central role of VEGF was confirmed by showing that normal levels of neovascularization (compared with C57) could be achieved in NOD mice that had been supplemented for this growth factor via intramuscular injection of an adenoviral vector encoding for VEGF. We conclude that 1) diabetes impairs endogenous neovascularization of ischemic tissues; 2) the impairment in new blood vessel formation results from reduced expression of VEGF; and 3) cytokine supplementation achieved by intramuscular adeno-VEGF gene transfer restores neovascularization in a mouse model of diabetes.  (+info)

Expression and cellular localization of the CC chemokines PARC and ELC in human atherosclerotic plaques. (8/12862)

Local immune responses are thought to play an important role in the development of atherosclerosis. Histological studies have shown that human atherosclerotic lesions contain T lymphocytes throughout all stages of development, many of which are in an activated state. A number of novel CC chemokines have been described recently, which are potent chemoattractants for lymphocytes: PARC (pulmonary and activation-regulated chemokine), ELC (EBI1-ligand chemokine), LARC (liver and activation-regulated chemokine), and SLC (secondary lymphoid-tissue chemokine). Using reverse transcriptase-polymerase chain reaction and in situ hybridization, we have found gene expression for PARC and ELC but not for LARC or SLC in human atherosclerotic plaques. Immunohistochemical staining of serial plaque sections with specific cell markers revealed highly different expression patterns of PARC and ELC. PARC mRNA was restricted to CD68+ macrophages (n = 14 of 18), whereas ELC mRNA was widely expressed by macrophages and intimal smooth muscle cells (SMC) in nearly all of the lesions examined (n = 12 of 14). ELC mRNA was also found to be expressed in the medial SMC wall of highly calcified plaques (n = 4). Very low levels of ELC mRNA expression could also be detected in normal mammary arteries but no mRNA expression for PARC was detected in these vessels (n = 4). In vitro, ELC mRNA was found to be up-regulated in aortic SMC stimulated with tumor necrosis factor-a and interferon-gamma but not in SMC stimulated with serum. Both PARC and ELC mRNA were expressed by monocyte-derived macrophages but not monocytes. The expression patterns of PARC and ELC mRNA in human atherosclerotic lesions suggest a potential role for these two recently described CC chemokines in attracting T lymphocytes into atherosclerotic lesions.  (+info)

Immunoenzyme techniques are a group of laboratory methods used in immunology and clinical chemistry that combine the specificity of antibody-antigen reactions with the sensitivity and amplification capabilities of enzyme reactions. These techniques are primarily used for the detection, quantitation, or identification of various analytes (such as proteins, hormones, drugs, viruses, or bacteria) in biological samples.

In immunoenzyme techniques, an enzyme is linked to an antibody or antigen, creating a conjugate. This conjugate then interacts with the target analyte in the sample, forming an immune complex. The presence and amount of this immune complex can be visualized or measured by detecting the enzymatic activity associated with it.

There are several types of immunoenzyme techniques, including:

1. Enzyme-linked Immunosorbent Assay (ELISA): A widely used method for detecting and quantifying various analytes in a sample. In ELISA, an enzyme is attached to either the capture antibody or the detection antibody. After the immune complex formation, a substrate is added that reacts with the enzyme, producing a colored product that can be measured spectrophotometrically.
2. Immunoblotting (Western blot): A method used for detecting specific proteins in a complex mixture, such as a protein extract from cells or tissues. In this technique, proteins are separated by gel electrophoresis and transferred to a membrane, where they are probed with an enzyme-conjugated antibody directed against the target protein.
3. Immunohistochemistry (IHC): A method used for detecting specific antigens in tissue sections or cells. In IHC, an enzyme-conjugated primary or secondary antibody is applied to the sample, and the presence of the antigen is visualized using a chromogenic substrate that produces a colored product at the site of the antigen-antibody interaction.
4. Immunofluorescence (IF): A method used for detecting specific antigens in cells or tissues by employing fluorophore-conjugated antibodies. The presence of the antigen is visualized using a fluorescence microscope.
5. Enzyme-linked immunosorbent assay (ELISA): A method used for detecting and quantifying specific antigens or antibodies in liquid samples, such as serum or culture supernatants. In ELISA, an enzyme-conjugated detection antibody is added after the immune complex formation, and a substrate is added that reacts with the enzyme to produce a colored product that can be measured spectrophotometrically.

These techniques are widely used in research and diagnostic laboratories for various applications, including protein characterization, disease diagnosis, and monitoring treatment responses.

... and Related Labeling Techniques, 7th International Conference; by Ernest H. Beutner,Russell J. Nisengard (Editor),Boris Albini ... "s; by Ernst H. Beutner; Dowden, Hutchinson & Ross, Incorporated; 1/1/1973 Defined Immunoflourescence, Immunoenzyme Studies, ...
... his team was the first in the former Soviet Union to implement immunoenzyme methods, monoclonal antibody technique, and flow ...
... immunoenzyme techniques MeSH E05.478.567.350.170 - enzyme-linked immunosorbent assay MeSH E05.478.567.350.180 - enzyme ... embryo culture techniques MeSH E05.200.249.484 - organ culture techniques MeSH E05.200.249.617 - tissue culture techniques MeSH ... immunoenzyme techniques MeSH E05.478.588.400.170 - enzyme-linked immunosorbent assay MeSH E05.478.588.400.180 - enzyme ... immunoenzyme techniques MeSH E05.601.495.350.170 - enzyme-linked immunosorbent assay MeSH E05.601.495.350.180 - enzyme ...
... immunoenzyme techniques MeSH E01.450.495.410.350.200 - enzyme-linked immunosorbent assay MeSH E01.450.495.410.350.210 - enzyme ... fluorescent antibody technique, direct MeSH E01.450.495.225.230 - fluorescent antibody technique, indirect MeSH E01.450.495.225 ... multiplied immunoassay technique MeSH E01.450.495.410.380 - immunosorbent techniques MeSH E01.450.495.410.380.200 - enzyme- ... glucose clamp technique MeSH E01.450.150.100.355 - glucose tolerance test MeSH E01.450.150.100.450 - lactose tolerance test ...
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Immunoenzyme Techniques * Male * Pituitary Neoplasms / analysis* * Pituitary Neoplasms / pathology * Pituitary Neoplasms / ...
Immunoenzyme Techniques * Infant * Lysine / analogs & derivatives* * Lysine / metabolism * Middle Aged * Pigment Epithelium of ...
We applied various machine learning techniques using the tf-idf and tf-rf schemes and their BM25 versions. Our results show ... System biology techniques provide novel insights into the pathogenesis, risk stratification, and clinical management in NKTCL. ... METHODS: Site-Directed Mutagenesis technique was employed to introduce the change RGD to RGE (RGD-to-RGE) within Fbln5 cDNA ... Immunoenzyme Techniques. *Chromosome 12. *Single Nucleotide Polymorphism. *Messenger RNA. *Xenograft Models. *Apoptosis ...
... and Related Labeling Techniques, 7th International Conference; by Ernest H. Beutner,Russell J. Nisengard (Editor),Boris Albini ... "s; by Ernst H. Beutner; Dowden, Hutchinson & Ross, Incorporated; 1/1/1973 Defined Immunoflourescence, Immunoenzyme Studies, ...
Categories: Immunoenzyme Techniques Image Types: Photo, Illustrations, Video, Color, Black&White, PublicDomain, ...
van der Loos CM, Das PK, Van den Oord JJ, et al. Multiple immunoenzyme staining techniques. Use of fluoresceinated, ...
OBJECTIVES: To report 4 cases of primary mucinous carcinoma of the eyelid, demonstrate how p63 expression aids diagnosis, and review the literature regarding the local recurrence potential of these eyelid tumors. METHODS: Since 1991, we have examined 4 cases of primary mucinous carcinoma of the eyelid. Histology slides were reviewed and clinical information was obtained from the medical records. Published cases of primary mucinous carcinoma involving the eyelid were identified using Ovid MEDLINE and PubMed and references within the articles. RESULTS: Our average patient age was 73 years, the female:male ratio was 3:1, and patients had a painless nodular mass for 1 month to 2 years. No patient had another documented malignant tumor at the time of diagnosis. All tumors were dermis based in the upper eyelid, three-fourths invaded the orbicularis oculi muscle, and the average tumor diameter was 3.7 mm. All tumors expressed cytokeratin 7 and gross cystic disease fluid protein 15 and were negative for ...
Indirect fluorescent antibody technique using sonicated Wuchereria bancrofti microfilaria for immunodiagnosis of Bancroftian ... Detection of IgE-binding Onchocerca volvulus antigens after electrophoretic transfer and immuno-enzyme reaction. Acta Tropica, ... Identification of immunogenic proteins of Dipetalonema viteae (Filarioidea) by the "Western Blotting" technique. Tropical ... Using this technique, the antigens that are cross-reactive with different human sera could have been identified by their ...
Immunoenzyme Techniques. en_HK. dc.subject.mesh. In Situ Hybridization - methods. en_HK. ... we have applied the technique of chromosome in situ hybridization in 13 cases of PGL by using archival paraffin-embedded tissue ... we have applied the technique of chromosome in situ hybridization in 13 cases of PGL by using archival paraffin-embedded tissue ...
keywords = "Antibodies, Monoclonal, Humans, Immunoenzyme Techniques, Killer Cells, Natural, Lymph Nodes, Lymphadenitis, ...
Viral/blood antibodies; Immunoenzyme techniques; Peromyscus; Sentinel surveillance; Sin Nombre virus. Place of Publication. ...
Immunoenzyme techniques, Immunological techniques, Peptides, Drug residues, Polymerase chain reaction, Legionella, Bioactive ... Diagnostic techniques, Immune serum, Immunity, Immunization, Immunoassay, Immunochemistry, Immunodiagnosis, ...
The immunoenzyme polymer technique, indirect immunofluorescence technique, Mouse on Mouse polymer IHC Package (Abcam plc., ... Cambridge, UK), and tagged streptavidin-biotin (LSAB) staining technique had been employed for anti-trypsin and anti- ...
Immunoenzyme Techniques (MeSH) * Male (MeSH) * Middle Aged (MeSH) * Mouth Mucosa (MeSH) * Mutation (MeSH) ...
Antibody Enzyme Technique, Unlabeled -- See Immunoenzyme Techniques Immunologic techniques based on the use of: (1) enzyme- ... Antibody Enzyme Technic, Unlabeled -- See Immunoenzyme Techniques Immunologic techniques based on the use of: (1) enzyme- ... Antibody Dissociation -- See Immunologic Techniques Techniques used to demonstrate or measure an immune response, and to ... Antibody Dissociations -- See Immunologic Techniques Techniques used to demonstrate or measure an immune response, and to ...
Immunoenzyme Techniques Medicine & Life Sciences 58% * O-Glycan Chemical Compounds 27% View full fingerprint ...
diagnostic techniques Agriculture & Biology 28% * Immunoenzyme Techniques Medicine & Life Sciences 28% * testing Agriculture & ...
ELISpot techniques are amongst the most-sensitive methods available (up to 400x more sensitive than conventional ELISA) for ... Utilising sandwich immuno-enzyme technology, Diaclone ELISpot and Dual ELISpot assays can detect both secreted cytokines and ... These techniques are amongst the most sensitive available for analyte identification and quantification and benefit from a ...
This technique is designed to determine the frequency of cytokine producing cells under a given stimulation, and the follow-up ... The ELISPOT assay is based on sandwich immuno-enzyme technology. Cell secreted cytokines or soluble molecules are captured by ...
Enzyme-linked immunosorbent assay; Helicobacter pylori; Immunoenzyme techniques; Serology. Citation. Korean Journal of ...
Immunoenzyme Techniques. 1. 2003. 207. 0.060. Why? Phylogeny. 1. 2006. 823. 0.060 ...
Immunoenzyme Techniques. dc.subject. Reverse Transcriptase Polymerase Chain Reaction. dc.subject. Adaptation, Physiological. ...
Immunoenzyme Techniques. Immunologic techniques based on the use of: (1) enzyme-antibody conjugates; (2) enzyme-antigen ... AdjuvantIncidental FindingsImmunoenzyme TechniquesEsophagectomySeverity of Illness IndexROC CurveIncidenceAntineoplastic ... A set of techniques used when variation in several variables has to be studied simultaneously. In statistics, multivariate ... An imaging technique using compounds labelled with short-lived positron-emitting radionuclides (such as carbon-11, nitrogen-13 ...
Immunoenzyme Techniques * Lung * Molecular Sequence Data * Placenta * Polymerase Chain Reaction * Pregnancy Identity. PubMed ...
  • Cambridge, UK), and tagged streptavidin-biotin (LSAB) staining technique had been employed for anti-trypsin and anti-carboxypeptidase A antibodies, anti-calreticulin antibody, anti-DDIT3 antibody, and anti-ATF4 antibody, respectively. (bioskinrevive.com)
  • These techniques are amongst the most sensitive available for analyte identification and quantification and benefit from a rapid whole-assay time and the provision of robustly validated standards, controls and antibodies. (caltagmedsystems.co.uk)
  • To study the incidence of trisomy 3 and its implications for the pathogenesis of PGL in Hong Kong, we have applied the technique of chromosome in situ hybridization in 13 cases of PGL by using archival paraffin-embedded tissue sections. (hku.hk)
  • The immunoenzyme polymer technique, indirect immunofluorescence technique, Mouse on Mouse polymer IHC Package (Abcam plc. (bioskinrevive.com)
  • HIV-1 p24 antigen was also detected in PM brain tissue by TSA enhanced immunofluorescence and demonstrated increased sensitivity compared to the conventional immunofluorescence technique with a greatly reduced autofluorescence background. (ox.ac.uk)
  • However, standard enzyme immunoassay techniques are not sufficiently sensitive for the measurement of some antigens from other viruses, bacteria, and parasites in concentrations that commonly occur in body fluids during the course of infectious diseases. (johnshopkins.edu)
  • was extracted using sequential extraction techniques for the partial separation of the herbicidal fraction. (bvsalud.org)
  • The use of such techniques should lead to the development of efficient enzyme immunoassay systems for the direct detection of a wide range of bacterial, viral, and parasitic infections. (johnshopkins.edu)
  • ELISpot techniques are amongst the most-sensitive methods available (up to 400x more sensitive than conventional ELISA) for cytokine research and immune monitoring. (caltagmedsystems.co.uk)
  • An imaging technique using compounds labelled with short-lived positron-emitting radionuclides (such as carbon-11, nitrogen-13, oxygen-15 and fluorine-18) to measure cell metabolism. (lookformedical.com)
  • Results of search for 'su:{Immunoenzyme techniques. (who.int)
  • Development and evaluation of modern enzyme immunoassays for comprehensive syphilis serology = Ontwikkeling en evaluatie van moderne enzym immunoassays voor toepassing in de verschillende aspecten van syfilisserologie / door Otto Emmanuel Ijsselmuiden. (who.int)
  • The latter samples were processed separately and simultaneously run, via a quantitative sandwich enzyme immunoassay technique (R&D Systems, Minneapolis, MN, USA). (usuhs.edu)
  • To identify or quantitate a normal serum protein component using a variety of immunodiffusion techniques including immunoelectrophoresis, single and double radial immunodiffusion and electroimmunodiffusion. (exalpha.com)
  • According to the types of labeling substances, such as fluorescent dyes, radioisotopes, enzymes (mainly horseradish peroxidase and alkaline phosphatase), ferritin, colloidal gold, etc., it can be divided into immunofluorescence , radioimmunoassay, immunoenzyme Labeling method and immune gold and silver method. (ballyabio.com)
  • As a blocking agent or as a negative control in nonprecipitating antibody-binding assays e.g. in serodiagnostic immunofluorescence and immunoenzyme tests. (exalpha.com)
  • A system of immunoenzyme analysis using biotinylated monoclonal antibodies for typing hantavirus antigens]. (nih.gov)
  • 15. Detection of anti-nuclear antibodies from filter paper blood clots using indirect immunoenzyme technique: preliminary experience and results. (nih.gov)
  • As a reference serum in nephelometry and other automated precipitation techniques. (exalpha.com)
  • Immunoenzyme Techniques" is a descriptor in the National Library of Medicine's controlled vocabulary thesaurus, MeSH (Medical Subject Headings) . (rush.edu)