Immunologic techniques based on the use of: (1) enzyme-antibody conjugates; (2) enzyme-antigen conjugates; (3) antienzyme antibody followed by its homologous enzyme; or (4) enzyme-antienzyme complexes. These are used histologically for visualizing or labeling tissue specimens.
The study of microorganisms such as fungi, bacteria, algae, archaea, and viruses.
A publication issued at stated, more or less regular, intervals.
Techniques used in microbiology.
A quantitative measure of the frequency on average with which articles in a journal have been cited in a given period of time.
Hospital facilities equipped to carry out investigative procedures.
"The business or profession of the commercial production and issuance of literature" (Webster's 3d). It includes the publisher, publication processes, editing and editors. Production may be by conventional printing methods or by electronic publishing.
A subtype of BRADYKININ RECEPTOR that is induced in response to INFLAMMATION. It may play a role in chronic inflammation and has a high specificity for KININS lacking the C-terminal ARGININE such as des-Arg(10)-kallidin and des-Arg(9)-bradykinin. The receptor is coupled to G-PROTEIN, GQ-G11 ALPHA FAMILY and G-PROTEIN, GI-GO ALPHA FAMILY signaling proteins.
A system of metabolic interactions by products produced in the distal nephron of the KIDNEY. These products include KALLIKREIN; KININS; KININASE I; KININASE II; and ENKEPHALINASE. This system participates in the control of renal functions. It interacts with the RENIN-ANGIOTENSIN-ALDOSTERONE SYSTEM to regulate BLOOD PRESSURE, generation of PROSTAGLANDINS, release of VASOPRESSINS, and WATER-ELECTROLYTE BALANCE.
A generic term used to describe a group of polypeptides with related chemical structures and pharmacological properties that are widely distributed in nature. These peptides are AUTACOIDS that act locally to produce pain, vasodilatation, increased vascular permeability, and the synthesis of prostaglandins. Thus, they comprise a subset of the large number of mediators that contribute to the inflammatory response. (From Goodman and Gilman's The Pharmacologic Basis of Therapeutics, 8th ed, p588)
A family of trypsin-like SERINE ENDOPEPTIDASES that are expressed in a variety of cell types including human prostate epithelial cells. They are formed from tissue prokallikrein by action with TRYPSIN. They are highly similar to PROSTATE-SPECIFIC ANTIGEN.
Proteolytic enzymes from the serine endopeptidase family found in normal blood and urine. Specifically, Kallikreins are potent vasodilators and hypotensives and increase vascular permeability and affect smooth muscle. They act as infertility agents in men. Three forms are recognized, PLASMA KALLIKREIN (EC 3.4.21.34), TISSUE KALLIKREIN (EC 3.4.21.35), and PROSTATE-SPECIFIC ANTIGEN (EC 3.4.21.77).
Ulceration of the GASTRIC MUCOSA due to contact with GASTRIC JUICE. It is often associated with HELICOBACTER PYLORI infection or consumption of nonsteroidal anti-inflammatory drugs (NSAIDS).
Ulcer that occurs in the regions of the GASTROINTESTINAL TRACT which come into contact with GASTRIC JUICE containing PEPSIN and GASTRIC ACID. It occurs when there are defects in the MUCOSA barrier. The common forms of peptic ulcers are associated with HELICOBACTER PYLORI and the consumption of nonsteroidal anti-inflammatory drugs (NSAIDS).
Tumors or cancer of the LUNG.
A heterogeneous aggregate of at least three distinct histological types of lung cancer, including SQUAMOUS CELL CARCINOMA; ADENOCARCINOMA; and LARGE CELL CARCINOMA. They are dealt with collectively because of their shared treatment strategy.
The use of two or more chemicals simultaneously or sequentially in the drug therapy of neoplasms. The drugs need not be in the same dosage form.
Evaluation undertaken to assess the results or consequences of management and procedures used in combating disease in order to determine the efficacy, effectiveness, safety, and practicability of these interventions in individual cases or series.
Proteins, glycoprotein, or lipoprotein moieties on surfaces of tumor cells that are usually identified by monoclonal antibodies. Many of these are of either embryonic or viral origin.
Carbohydrate antigens expressed by malignant tissue. They are useful as tumor markers and are measured in the serum by means of a radioimmunoassay employing monoclonal antibodies.
INFLAMMATION of the LIVER in humans caused by a member of the ORTHOHEPADNAVIRUS genus, HEPATITIS B VIRUS. It is primarily transmitted by parenteral exposure, such as transfusion of contaminated blood or blood products, but can also be transmitted via sexual or intimate personal contact.
Those hepatitis B antigens found on the surface of the Dane particle and on the 20 nm spherical and tubular particles. Several subspecificities of the surface antigen are known. These were formerly called the Australia antigen.
The type species of the genus ORTHOHEPADNAVIRUS which causes human HEPATITIS B and is also apparently a causal agent in human HEPATOCELLULAR CARCINOMA. The Dane particle is an intact hepatitis virion, named after its discoverer. Non-infectious spherical and tubular particles are also seen in the serum.
INFLAMMATION of the LIVER with ongoing hepatocellular injury for 6 months or more, characterized by NECROSIS of HEPATOCYTES and inflammatory cell (LEUKOCYTES) infiltration. Chronic hepatitis can be caused by viruses, medications, autoimmune diseases, and other unknown factors.
Antibodies to the HEPATITIS B ANTIGENS, including antibodies to the surface (Australia) and core of the Dane particle and those to the "e" antigens.
A group of heterogeneous lymphoid tumors generally expressing one or more B-cell antigens or representing malignant transformations of B-lymphocytes.
INFLAMMATION of the LIVER in humans caused by HEPATITIS C VIRUS, a single-stranded RNA virus. Its incubation period is 30-90 days. Hepatitis C is transmitted primarily by contaminated blood parenterally, and is often associated with transfusion and intravenous drug abuse. However, in a significant number of cases, the source of hepatitis C infection is unknown.
Clonal hematopoietic stem cell disorders characterized by dysplasia in one or more hematopoietic cell lineages. They predominantly affect patients over 60, are considered preleukemic conditions, and have high probability of transformation into ACUTE MYELOID LEUKEMIA.
Clonal expansion of myeloid blasts in bone marrow, blood, and other tissue. Myeloid leukemias develop from changes in cells that normally produce NEUTROPHILS; BASOPHILS; EOSINOPHILS; and MONOCYTES.
Form of leukemia characterized by an uncontrolled proliferation of the myeloid lineage and their precursors (MYELOID PROGENITOR CELLS) in the bone marrow and other sites.
The worsening of a disease over time. This concept is most often used for chronic and incurable diseases where the stage of the disease is an important determinant of therapy and prognosis.
Active immunization where vaccine is administered for therapeutic or preventive purposes. This can include administration of immunopotentiating agents such as BCG vaccine and Corynebacterium parvum as well as biological response modifiers such as interferons, interleukins, and colony-stimulating factors in order to directly stimulate the immune system.
A tumor derived from mesothelial tissue (peritoneum, pleura, pericardium). It appears as broad sheets of cells, with some regions containing spindle-shaped, sarcoma-like cells and other regions showing adenomatous patterns. Pleural mesotheliomas have been linked to exposure to asbestos. (Dorland, 27th ed)
A protein-tyrosine kinase receptor that is closely related in structure to the INSULIN RECEPTOR. Although commonly referred to as the IGF-I receptor, it binds both IGF-I and IGF-II with high affinity. It is comprised of a tetramer of two alpha and two beta subunits which are derived from cleavage of a single precursor protein. The beta subunit contains an intrinsic tyrosine kinase domain.
The original member of the family of endothelial cell growth factors referred to as VASCULAR ENDOTHELIAL GROWTH FACTORS. Vascular endothelial growth factor-A was originally isolated from tumor cells and referred to as "tumor angiogenesis factor" and "vascular permeability factor". Although expressed at high levels in certain tumor-derived cells it is produced by a wide variety of cell types. In addition to stimulating vascular growth and vascular permeability it may play a role in stimulating VASODILATION via NITRIC OXIDE-dependent pathways. Alternative splicing of the mRNA for vascular endothelial growth factor A results in several isoforms of the protein being produced.
A family of closely related RECEPTOR PROTEIN-TYROSINE KINASES that bind vascular endothelial growth factors. They share a cluster of seven extracellular Ig-like domains which are important for ligand binding. They are highly expressed in vascular endothelial cells and are critical for the physiological and pathological growth, development and maintenance of blood and lymphatic vessels.
A 200-230-kDa tyrosine kinase receptor for vascular endothelial growth factors found primarily in endothelial and hematopoietic cells and their precursors. VEGFR-2 is important for vascular and hematopoietic development, and mediates almost all endothelial cell responses to VEGF.
A family of angiogenic proteins that are closely-related to VASCULAR ENDOTHELIAL GROWTH FACTOR A. They play an important role in the growth and differentiation of vascular as well as lymphatic endothelial cells.
Neoplasms of the thin serous membrane that envelopes the lungs and lines the thoracic cavity. Pleural neoplasms are exceedingly rare and are usually not diagnosed until they are advanced because in the early stages they produce no symptoms.
A group of malignant lymphomas thought to derive from peripheral T-lymphocytes in lymph nodes and other nonlymphoid sites. They include a broad spectrum of lymphocyte morphology, but in all instances express T-cell markers admixed with epithelioid histiocytes, plasma cells, and eosinophils. Although markedly similar to large-cell immunoblastic lymphoma (LYMPHOMA, LARGE-CELL, IMMUNOBLASTIC), this group's unique features warrant separate treatment.
A group of heterogeneous lymphoid tumors representing malignant transformations of T-lymphocytes.
A general term for various neoplastic diseases of the lymphoid tissue.
They are oval or bean shaped bodies (1 - 30 mm in diameter) located along the lymphatic system.
A group of lymphomas exhibiting clonal expansion of malignant T-lymphocytes arrested at varying stages of differentiation as well as malignant infiltration of the skin. MYCOSIS FUNGOIDES; SEZARY SYNDROME; LYMPHOMATOID PAPULOSIS; and PRIMARY CUTANEOUS ANAPLASTIC LARGE CELL LYMPHOMA are the best characterized of these disorders.
CXCR receptors with specificity for CXCL12 CHEMOKINE. The receptors may play a role in HEMATOPOIESIS regulation and can also function as coreceptors for the HUMAN IMMUNODEFICIENCY VIRUS.
The medical science concerned with the prevention, diagnosis, and treatment of diseases in animals.
An immunoassay utilizing an antibody labeled with an enzyme marker such as horseradish peroxidase. While either the enzyme or the antibody is bound to an immunosorbent substrate, they both retain their biologic activity; the change in enzyme activity as a result of the enzyme-antibody-antigen reaction is proportional to the concentration of the antigen and can be measured spectrophotometrically or with the naked eye. Many variations of the method have been developed.
Discussion of lists of works, documents or other publications, usually with some relationship between them, e.g., by a given author, on a given subject, or published in a given place, and differing from a catalog in that its contents are restricted to holdings of a single collection, library, or group of libraries. (from The ALA Glossary of Library and Information Science, 1983)
A list of works, documents, and other publications on medical subjects and topics of interest to the field of medicine.
Use for general articles concerning veterinary medical education.
Educational institutions for individuals specializing in the field of veterinary medicine.

Immunohistochemical expression of mdm2 and p21WAF1 in invasive cervical cancer: correlation with p53 protein and high risk HPV infection. (1/12862)

AIM: To investigate the immunocytochemical staining pattern of mdm2 and p21WAF1 proteins in invasive cervical cancer and to determine its relation with the expression of p53 and with the high risk HPV infection. METHODS: Immunocytochemistry for p53, mdm2, and p21WAF1 was performed in 31 paraffin embedded sections of invasive cervical cancer. The results were assessed by image analysis, evaluating for each protein the optical density of the immunostained area, scored as percentage of the total nuclear area. The presence of high risk human papillomavirus (HPV) infection was detected by using the polymerase chain reaction. RESULTS: Immunostaining for both mdm2 and p21WAF1 was correlated with p53 expression; however, the correlation between p53 and mdm2 (R = 0.49; p < 0.01) was more significant than between p53 and p21WAF1 (R = 0.31; p < 0.05); the less stringent correlation between p53 and p21WAF1 might reflect the p53 independent mechanisms of p21WAF1 induction. Similar average levels of p53, mdm2, and p21WAF1 immunostaining were found in the presence or absence of high risk HPV-DNA, without significant differences between the two groups. CONCLUSIONS: These data suggest that mdm2 and p21WAF1 proteins are expressed in invasive cervical cancer and that their immunocytochemical staining pattern is not abrogated by the presence of high risk HPV genomic sequences.  (+info)

Expression of vascular endothelial growth factor in human oral squamous cell carcinoma: its association with tumour progression and p53 gene status. (2/12862)

AIMS: To correlate vascular endothelial growth factor (VEGF) expression in oral squamous cell carcinoma with the clinicopathological characteristics and prognosis; and to assess whether p53 gene status is associated with VEGF expression in human cancers. METHODS: Tumour specimens from 45 patients with oral squamous cell carcinomas were examined. Expression of VEGF was determined using an immunohistochemical method, and a tumour was considered positive when more than 5% of the neoplastic cells showed VEGF immunoreactivity. The p53 gene status was screened using a polymerase chain reaction--single strand conformation polymorphism analysis. RESULTS: VEGF positive staining was detected in 19 (42.2%) of the 45 cases. VEGF immunoreactivity did not correlate with the histological degree of tumour differentiation, clinical stages, or lymph node metastasis. The patients with VEGF positive tumours had a significantly worse prognosis than those with VEGF negative tumours. The five year overall survival rate of the VEGF negative patients was 76.5%, as compared with 48.8% for the VEGF positive patients. No significant association between VEGF expression and the p53 gene status of the tumours was found. CONCLUSIONS: VEGF is a good prognostic indicator of the survival of patients with oral squamous cell carcinoma. The p53 gene status does not seem to be associated with VEGF expression in these cancers.  (+info)

Overexpression of human homologs of the bacterial DnaJ chaperone in the synovial tissue of patients with rheumatoid arthritis. (3/12862)

OBJECTIVE: To study the expression of the chaperone family of J proteins in the synovial tissue of patients with rheumatoid arthritis (RA) or osteoarthritis. METHODS: Rabbit antibodies specific for a synthetic peptide (pHSJ1: EAYEVLSDKHKREIYD), representing the most conserved part of all J domains thus far identified--among them the Drosophila tumor suppressor Tid56--were used in immunohistochemical analyses of frozen sections of synovial tissue and immunoblotting of protein extracts of adherent synovial cells. IgG specific for Tid56 was also used. RESULTS: Both antisera predominantly and intensely stained synovial lining cells from RA patients; other cells did not stain or stained only faintly. In immunoblots, anti-pHSJ1 specifically detected several bands with molecular weights of >74 kd (type I), 57-64 kd (type II), 41-48 kd (type III), and < or =36 kd (type IV). The strongest band detected in RA adherent synovial cells was the type II band, whereas in a B cell line, a type I band was prominent. CONCLUSION: Several potentially new members of the J family are described. The type II band represents the human homolog of the Drosophila Tid56 protein and is strongly expressed in RA synovial tissue.  (+info)

Expression of B7 costimulatory molecules by salivary gland epithelial cells in patients with Sjogren's syndrome. (4/12862)

OBJECTIVE: To investigate the expression of B7 costimulatory molecules in the lymphoepithelial lesions of salivary gland (SG) biopsy tissues and in SG epithelial cell lines derived from patients with Sjogren's syndrome (SS). METHODS: B7.1 and B7.2 protein expression was studied by immunohistochemistry in minor SGs obtained from 11 patients with SS and 10 disease control patients with nonspecific sialadenitis and in cultured SG epithelial cell lines obtained from minor SGs from 15 SS patients and 15 control patients. B7.1 and B7.2 messenger RNA (mRNA) expression by SG epithelial cell lines was examined by reverse transcription-polymerase chain reaction (RT-PCR). RESULTS: In biopsy tissues from SS patients, but not control patients, ductal and acinar epithelial cells showed increased expression of both B7.1 and B7.2. Intense spontaneous B7.1 protein expression (as well as HLA-ABC, but not B7.2 or HLA-DR) was also found in 73% of SG epithelial cell lines from SS patients versus 13% of those from control patients (P < 0.01). Interferon-y treatment induced, or up-regulated, B7.1, B7.2, and HLA-DR expression in all SG epithelial cell lines tested. B7.1 and B7.2 expression by SG epithelial cell lines was also verified at the mRNA level by RT-PCR. CONCLUSION: Human SG epithelia are intrinsically capable of expressing B7 proteins upon activation. In SS patients, the expression of B7 molecules by SG epithelial tissues and by SG epithelial cell lines indicates the activated status of SG epithelial cells in this disorder and, possibly, their capacity for presenting antigens to T cells.  (+info)

Localization of sodium bicarbonate cotransporter (NBC) protein and messenger ribonucleic acid in rat epididymis. (5/12862)

An acidic environment is important for sperm maturation in the epididymis and also helps to maintain mature sperm in an immotile state during storage in this organ. Both an Na+/H+ exchanger and an H+ATPase have been implicated in this process. The H+ATPase is concentrated in specialized apical (and/or narrow) and clear cells of the epididymis, while the Na+/H+ exchanger has not yet been localized in situ. As in other proton-secreting epithelia, bicarbonate transport occurs in the epididymis, where it is implicated in luminal acidification. In this study we used an antibody raised against a fusion protein (maltose-binding protein: MBP-NBC-5) from the C-terminus of the recently cloned rat kidney Na+/HCO3- cotransporter (NBC) to localize this protein in the epididymis and vas deferens of the rat. The distribution of the respective mRNA was mapped by in situ hybridization. NBC message was strongly expressed in the initial segment and the intermediate zone of the epididymis, and the NBC-5 antibody gave a strong basolateral staining in both principal cells and apical/narrow cells in this region. Western blotting revealed a single band at about 160 kDa in the epididymis. The intensity of staining as well as mRNA levels decreased in the cauda epididymidis and in the vas deferens, where only weak staining was seen. Basolateral NBC may function in parallel with apical proton secretion to regulate luminal acidification and/or bicarbonate reabsorption in the excurrent duct system.  (+info)

Epithelial hyperproliferation and transglutaminase 1 gene expression in Stevens-Johnson syndrome conjunctiva. (6/12862)

In Stevens-Johnson syndrome, pathological keratinization of the ordinarily nonkeratinized corneal and conjunctival mucosal epithelia results in severe visual loss. We examined conjunctiva covering cornea in five eyes in the chronic cicatricial phase of Stevens-Johnson syndrome. Normal conjunctiva from five age-matched individuals was studied also. The number of epithelial cells in Stevens-Johnson syndrome conjunctiva that were immunoreactive with a monoclonal antibody, Ki-67, to a nuclear antigen found only in proliferating cells was greater than normal (93.8+/-19.8 cells above 100 basal cells versus 12.8+/-0.5 cells above 100 basal cells; P = 0.009). In addition, although clinical inflammation was mild, massive lymphocytic infiltration was seen in the substantia propria of conjunctiva covering cornea. In situ hybridization documented transglutaminase 1 (keratinocyte transglutaminase) mRNA in suprabasal cells of the abnormally thickened conjunctival epithelium in all Stevens-Johnson syndrome patients. In contrast, no message was detected in normal conjunctival or corneal epithelia. Transglutaminase 1 is expressed during the terminal differentiation of keratinocytes where it helps synthesize cornified cell envelopes. We speculate that in Stevens-Johnson syndrome, epithelial hyperproliferation, and transglutaminase 1 gene expression lead to the pathological keratinization of ocular surface mucosal epithelia.  (+info)

Rescue of diabetes-related impairment of angiogenesis by intramuscular gene therapy with adeno-VEGF. (7/12862)

Diabetes is a major risk factor for coronary and peripheral artery diseases. Although diabetic patients often present with advanced forms of these diseases, it is not known whether the compensatory mechanisms to vascular ischemia are affected in this condition. Accordingly, we sought to determine whether diabetes could: 1) impair the development of new collateral vessel formation in response to tissue ischemia and 2) inhibit cytokine-induced therapeutic neovascularization. Hindlimb ischemia was created by femoral artery ligation in nonobese diabetic mice (NOD mice, n = 20) and in control C57 mice (n = 20). Hindlimb perfusion was evaluated by serial laser Doppler studies after the surgery. In NOD mice, measurement of the Doppler flow ratio between the ischemic and the normal limb indicated that restoration of perfusion in the ischemic hindlimb was significantly impaired. At day 14 after surgery, Doppler flow ratio in the NOD mice was 0.49+/-0.04 versus 0.73+/-0.06 for the C57 mice (P< or =0.005). This impairment in blood flow recovery persisted throughout the duration of the study with Doppler flow ratio values at day 35 of 0.50+/-0.05 versus 0.90+/-0.07 in the NOD and C57 mice, respectively (P< or =0.001). CD31 immunostaining confirmed the laser Doppler data by showing a significant reduction in capillary density in the NOD mice at 35 days after surgery (302+/-4 capillaries/mm2 versus 782+/-78 in C57 mice (P< or =0.005). The reduction in neovascularization in the NOD mice was the result of a lower level of vascular endothelial growth factor (VEGF) in the ischemic tissues, as assessed by Northern blot, Western blot and immunohistochemistry. The central role of VEGF was confirmed by showing that normal levels of neovascularization (compared with C57) could be achieved in NOD mice that had been supplemented for this growth factor via intramuscular injection of an adenoviral vector encoding for VEGF. We conclude that 1) diabetes impairs endogenous neovascularization of ischemic tissues; 2) the impairment in new blood vessel formation results from reduced expression of VEGF; and 3) cytokine supplementation achieved by intramuscular adeno-VEGF gene transfer restores neovascularization in a mouse model of diabetes.  (+info)

Expression and cellular localization of the CC chemokines PARC and ELC in human atherosclerotic plaques. (8/12862)

Local immune responses are thought to play an important role in the development of atherosclerosis. Histological studies have shown that human atherosclerotic lesions contain T lymphocytes throughout all stages of development, many of which are in an activated state. A number of novel CC chemokines have been described recently, which are potent chemoattractants for lymphocytes: PARC (pulmonary and activation-regulated chemokine), ELC (EBI1-ligand chemokine), LARC (liver and activation-regulated chemokine), and SLC (secondary lymphoid-tissue chemokine). Using reverse transcriptase-polymerase chain reaction and in situ hybridization, we have found gene expression for PARC and ELC but not for LARC or SLC in human atherosclerotic plaques. Immunohistochemical staining of serial plaque sections with specific cell markers revealed highly different expression patterns of PARC and ELC. PARC mRNA was restricted to CD68+ macrophages (n = 14 of 18), whereas ELC mRNA was widely expressed by macrophages and intimal smooth muscle cells (SMC) in nearly all of the lesions examined (n = 12 of 14). ELC mRNA was also found to be expressed in the medial SMC wall of highly calcified plaques (n = 4). Very low levels of ELC mRNA expression could also be detected in normal mammary arteries but no mRNA expression for PARC was detected in these vessels (n = 4). In vitro, ELC mRNA was found to be up-regulated in aortic SMC stimulated with tumor necrosis factor-a and interferon-gamma but not in SMC stimulated with serum. Both PARC and ELC mRNA were expressed by monocyte-derived macrophages but not monocytes. The expression patterns of PARC and ELC mRNA in human atherosclerotic lesions suggest a potential role for these two recently described CC chemokines in attracting T lymphocytes into atherosclerotic lesions.  (+info)

TY - JOUR. T1 - A one-step sandwich enzyme immunoassay for inactive precursor and complexed forms of human matrix metalloproteinase 9 (92 kDa gelatinase/type IV collagenase, gelatinase B) using monoclonal antibodies. AU - Fujimoto, Noboru. AU - Hosokawa, Nobuko. AU - Iwata, Kazushi. AU - Shinya, Takashi. AU - Okada, Yasunori. AU - Hayakawa, Taro. PY - 1994/11. Y1 - 1994/11. KW - Matrix metalloproteinase 9. KW - Monoclonal antibody. KW - Sandwich enzyme immunoassay. KW - Tissue inhibitor of metalloproteinases 1. UR - http://www.scopus.com/inward/record.url?scp=0027971387&partnerID=8YFLogxK. UR - http://www.scopus.com/inward/citedby.url?scp=0027971387&partnerID=8YFLogxK. U2 - 10.1016/0009-8981(94)90256-9. DO - 10.1016/0009-8981(94)90256-9. M3 - Article. C2 - 7704951. AN - SCOPUS:0027971387. VL - 231. SP - 79. EP - 88. JO - Clinica Chimica Acta. JF - Clinica Chimica Acta. SN - 0009-8981. IS - 1. ER - ...
Gentaur molecular products has all kinds of products like :search , BBridge \ Leptin ELISA Kit, Human A sandwich enzyme immunoassay using a sensitive polyclonal primary antibody quantitatively measures human leptin in serum, plasma, and tissue culture medium. Easily detect conc \ K1005-1 for more molecular products just contact us
Currently available enzyme immunoassay methods for peptides can be divided into two groups, homogeneous and heterogeneous methods. Homogeneous enzyme immunoassay methods, in which signals that are directly obtained from a mixture of test samples and reagents correlate with the amount of peptide in test samples, are simpler and quicker but less sensitive than heterogeneous enzyme immunoassay methods, in which free and bound forms of enzyme-labeled reactants are separated from each other.
Carceller, A.; Torres-Rodríguez, J.M.; Lowinger, M.; Alía, C., 1992: Standardized immunoenzyme analysis for detection of IgG antibodies against Candida albicans in systemic candidiasis
TY - JOUR. T1 - Production of monoclonal antibodies to ferritin and development of the enzyme immunoassay system. AU - Nozawa, S.. AU - Tsukazaki, K.. AU - Narisawa, S.. PY - 1985. Y1 - 1985. UR - http://www.scopus.com/inward/record.url?scp=0022353283&partnerID=8YFLogxK. UR - http://www.scopus.com/inward/citedby.url?scp=0022353283&partnerID=8YFLogxK. M3 - Article. C2 - 3910740. AN - SCOPUS:0022353283. VL - 37. SP - 2775. EP - 2783. JO - Nippon Sanka Fujinka Gakkai zasshi. JF - Nippon Sanka Fujinka Gakkai zasshi. SN - 0300-9165. IS - 12. ER - ...
Hepatitis A. HAV Ab. Competitive Enzyme ImmunoAssay (ELISA) for the determination of antibodies to Hepatitis A Virus in human plasma and sera.. HAV IgM. Enzyme ImmunoAssay (ELISA) for the determination of IgM class antibodies to Hepatitis A Virus in human plasma and sera.... Hepatitis B. HBc Ab. Competitive Enzyme ImmunoAssay (ELISA) for the determination of antibodies to Hepatitis B core Antigen in human plasma and sera.. HBc IgM. Enzyme ImmunoAssay (ELISA) for both the quantitative and qualitative determination of antibodies.... HBe Ag/Ab. Enzyme ImmunoAssay (ELISA) for the determination of Hepatitis B Virus \e\ Antigen and Antibody in human plasma and sera.. HBs Ab. Enzyme ImmunoAssay (ELISA) for both the quantitative and qualitative determination of antibodies.... HBs Ag. Third generation Enzyme Immunoassay for the determination of Hepatitis B surface Antigen or HBsAg in human serum and plasma.. HBs Ag Conf.. Third generation Enzyme Immunoassay for the determination of Hepatitis B surface ...
specificalIntended Uses: This CRYalphaB ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human CRYalphaB. This ELISA kit for research use only, not for therapeutic or diagnostic applications! Principle of the Assay,,CRYalphaB ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for CRYalphaB. Standards or samples are then added to the microtiter plate wells and CRYalphaB if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of CRYalphaB present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for CRYalphaB are added to each well to sandwich the CRYalphaB immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. ...
OUTLINE: Patients receive oral dasatinib twice daily on days 1-28. Treatment repeats every 28 days in the absence of disease progression or unacceptable toxicity.. Patients undergo tumor tissue and blood sample collection periodically for correlative studies. Tumor tissue samples are analyzed for EphA2 and PDGFRβ expression by immunohistochemistry. Tumor tissue samples may also be analyzed for phosphorylation of Src, EphA2, and PDGFRβ by western blot. Blood samples are analyzed for concentration of VEGF and PDGF by quantitative sandwich enzyme immunoassay technique; mesothelin-related protein level by Mesomark® assay; CSF-1 level by ELISA assay; and phosphorylation of Src by phospho-Src (pTyr418) human ELISA.. After completion of study treatment, patients are followed at least every 2 months for 1 year, then every 4 months for 1 year, then every 6 months for 1 year. ...
specificalPrinciple of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for MIB2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MIB2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MIB2 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MIB2 bound in the initial step. The color development is stopped and the intensity of the color is measured. ...
A new Enzyme ImmunoAssay (EIA) for PCDD/F TEQ measurement in extracts of environmental samples was described. The bioassay TEQ which derived from EIA and EROD were compared with each other and with results from chemical analysis. For all environmental samples, the EROD-TEQ is higher than the value from chemical analysis. However, the EIA-TEQ is much more identical with the value from chemical analysis. Our results indicate that the EIA assay is a complementary method to the EROD assay and should be useful as a rapid and sensitive screening tool for environmental samples in many situations. (C) 1999 Elsevier Science Ltd. All rights ...
Principle of the Assay This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for IL-15 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IL-15 present is bound by the immobilized antibody. Following incubation unbound sam
THE CYCLIC NUCLEOTIDES, CAMP AND CGMP ARE IMPORTANT REGULATORY MOLECULES THAT ARE NOW RECOGNIZED AS MEDIATORS IN A VAST NUMBER OF NORMAL AND PATHOLOGICAL PROCESSES. SUCH INSIGHTS HAVE INCREASED THE NEED FOR GENERALLY AVAILABLE TECHNOLOGY THAT WILL ALLOW THE CONVENIENT AND ACCURATE MEASUREMENT OF THE CYCLIC NUCLEOTIDES IN RESEARCH AND CLINICAL SETTINGS. THIS PHASE I PROPOSAL OUTLINES OUR INTEREST IN PURSUING THE GENERATION OF MURINE MONOCLONAL ANTI CAMP/CGMP ANTIBODIES AND FLUORESCENT ENZYME IMMUNOASSAY SYSTEMS FOR THE RAPID AND SENSITIVE MEASUREMENT OF THE CYCLIC NUCLEOTIDES IN BIOLOGICAL MATERIALS. HYBRIDOMAS WILL BE PREPARED VIA FUSION OF MOUSE MYELOMA CELLS WITH SPLEENS OF MICE IMMUNIZED WITH SUCCINYL-CAMP (OR CGMP) CONJUGATED TO BOVINE SERUM ALBUMIN. RESULTANT MONOCLONAL ANTIBODIES WILL BE ATTACHED TO BETA-GALACTOSIDASE AND UTILIZED IN A RAPID SOLID-PHASE ENZYME IMMUNOASSAY USING FLUOROGENIC SUBSTRATES. THESE EXPECTED RESULTS WILL SERVE AS THE BASIS OF PHASE II, WHERE WE ENVISION THAT THE ...
Quantitative sandwich enzyme immunoassay (EIA) systems, that can distinguish between active-form subtypes of mitogen-activated protein kinases (p44 and p42 MAP kinase, also called ERK1 and ERK2), were developed employing subtype-specific antibodies a
The invention concerns a method for the detection of an analyte in a sample liquid by an enzyme-immunoassay in which an enzyme-labelled compound is partitioned between a solid and a liquid phase and the amount of enzyme label in the liquid phase outside the solid phase is determined as a measure of the concentration of the analyte. The measurement is carried out in a non-porous molding having the liquid phase contained therein in contact with the solid phase. The method is particularly suitable for carrying out in a cuvette which can be filled via the porous matrix or on a test strip having a space in contact with the solid phase.
ASM journals are the most prominent publications in the field, delivering up-to-date and authoritative coverage of both basic and clinical microbiology.. About ASM , Contact Us , Press Room. ASM is a member of. ...
Due to the fact that the child is most often exposed to helminth infection, and the study of feces for parasite eggs is not always sufficiently informative, parents are usually recommended that children with suspected disability submit a general blood test andEIA.It is they who with the greatest accuracy will help to define worms in childrens organisms. Most of all, the helminths, such as pinworms and ascarids, affect the change in the indices in this study conducted in children. They can be determined by reduced hemoglobin or elevated white blood cells. Parents should know that it is possible to take a blood test for worms from a child in a private or public laboratory. The prices for this procedure in both institutions are approximately the same and quite low. The only thing that is required is the availability of special equipment that is designed to identify various types of worms in the childs body by blood. Also, remember that the baby should not be fed for 8 hours before the test. He ...
RayBio® EIA kits utilize the principle of Competitive Enzyme Immunoassay EIA in which the target protein and a biotin-conjugated peptide bind competitively to a capture antibody This assay requires only a single antibody generating signal output by interaction of the biotinylated competitor
TRANSIA PLATE Salmonella Gold is a sandwich enzyme immunoassay for the detection of Salmonella in food, feed and environmental samples. The test utilizes highly specific proprietary antibodies to create an antigen antibody complex which, if present, creates a color change reaction upon addition of the substrate which is read with a microplate reader. Standardized Protocol - Results in 24 hours : ...
Elisa kits,il-6 Elisa Kit,assay kit protein sandwich enzyme immunoassay for research scientists, vitro quantitative measurement of serum, Plasma , Urine ,tissue homogenates and Cell culture supernates
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TY - JOUR. T1 - Comparison of three anti-coccidioides antibody enzyme immunoassay kits for the diagnosis of coccidioidomycosis. AU - Malo, Joshua. AU - Holbrook, Eric. AU - Zangeneh, Tirdad. AU - Strawter, Chris. AU - Oren, Eyal -. AU - Robey, Ian. AU - Erickson, Heidi. AU - Carranza-Chahal, Racquel. AU - Durkin, Michelle. AU - Thompson, Cindy. AU - Hoover, Susan E.. AU - Ampel, Neil M.. AU - Wheat, L. Joseph. AU - Knox, Kenneth S.. PY - 2020/8/1. Y1 - 2020/8/1. N2 - Coccidioidomycosis is a common cause of community-acquired pneumonia in endemic areas of the southwestern United States. Clinical presentations range from self-limited disease to severe, disseminated disease. As such, early and accurate diagnosis is essential to ensure appropriate treatment and monitoring. Currently available diagnostic testing has variable accuracy, particularly in certain patient populations, and new tests may offer improved accuracy for the diagnosis of coccidioidomycosis. Serum samples from patients with ...
Associations between house dust-associated beta-(1,3)-glucan exposure and airway inflammatory reactions have been reported, while such exposures in early childhood have been suggested to protect against asthma and wheezing. Most epidemiological studies have used reservoir dust samples and an inhibition enzyme immunoassay (EIA) for beta-(1,3)-glucan exposure assessment. The objective of this ... read more study was to develop inexpensive but highly sensitive enzyme immunoassays to measure airborne beta-(1,3)-glucans in low-exposure environments, like homes. Specificities of available anti-beta-(1,3)-glucan antibodies were defined by direct and inhibition experiments. Three suitable antibody combinations were selected for sandwich EIAs. beta-(1,3)-Glucans in passive airborne dust collected with an electrostatic dust fall collector (EDC) and floor dust from seven homes were measured with the three EIAs. Floor dust samples were additionally analyzed in the inhibition EIA. The sandwich EIAs were ...
Results obtained with a recently introduced enzyme immunoassay system (EIA) for the detection of cryptococcal antigen (Meridian Diagnostics Inc) were compared with those obtained by a latex agglutination (LA) method (Immuno-Mycologics, Norman, Oklahoma, USA). Fifty four samples were examined. There was 92% agreement between the two methods. One false positive result was obtained with LA, and one sample was inevaluable. The EIA was rapid and simple to perform. There was some evidence that it gave fewer false positive reactions and improved the diagnosis of genuine early cases.. ...
Plasma exchange to remove HIT antibodies: dissociation between enzyme-immunoassay and platelet activation test reactivities Academic Article ...
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In a previous study employing conventional immunological marker analysis we found that 17% of high grade malignant lymphomas were devoid of cytoplasmic and membrane immunoglobulin and also sheep erythrocyte receptors. Cryostat sections from 24 of these cases (four of low grade and 20 of high grade malignancy) were stained with a panel of 30 monoclonal antibodies and six polyclonal antisera using a sensitive immunoperoxidase method. All tumours expressed the leucocyte common antigen (detected by monoclonal antibody 2D1) and all lacked epithelial cytokeratin (monoclonal antibody LE61), confirming their haematopoietic origin. All but one of the lymphomas expressed antigens characteristic of either B cells (17 cases) or T cells (six cases), while one case (morphologically a centroblastic lymphoma) had an unusual dual phenotype in which strong staining for T6 (marker of immature T cells) was associated with expression of the pan B lymphocyte antigens detectable with To15, anti-B1, anti-Leu12. This ...
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Purified viral lysates can be used in the manufacture of detection systems using solid-phase enzyme immunoassay, Western blotting, dot-blotting and related techniques. The material is valuable as an immunogen and for the purification of viral proteins.
Estrone EIA Reproductive Markers 011-CAN-E-420,The procedure follows the basic principle of a competitive enzyme immunoassay where there is competition between an unlabeled antigen and an labelled antigen bound to the limited binding sites of a specific antiserum . The amount of enzyme conjugate complex (HRP-avidin: biotin-labelled estrone) bou,medicine,medical supply,medical supplies,medical product
Hiramoto, R; Jurandowski, J; Bernecky, J; and Pressman, D, Immunohistochemical identification of tissue culture cells. (1961). Subject Strain Bibliography 1961. 1057 ...
Human frozen tissue section matched pair products include: Primary Pair (PP), Primary and Metastasis Pair (PM). PP consists of frozen tissue section of primary tumor and its adjacent normal tissue; PM consists of frozen tissue section of primary tumor and corresponding metastatic tumor. The frozen tissue sections in each pair are prepared from the same donor ...
The MaxSignal® Oxolinic Acid ELISA Kit is a competitive enzyme immunoassay for the quantitative analysis of Oxolinic Acid in shrimp, fish, and meat.
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MeSH-minor] Adenocarcinoma / drug therapy. Adenocarcinoma / metabolism. Adenocarcinoma / secondary. Adenocarcinoma, Clear Cell / drug therapy. Adenocarcinoma, Clear Cell / metabolism. Adenocarcinoma, Clear Cell / secondary. Adenocarcinoma, Mucinous / drug therapy. Adenocarcinoma, Mucinous / metabolism. Adenocarcinoma, Mucinous / secondary. Adult. Aged. Angiotensin II Type 1 Receptor Blockers / pharmacology. Animals. Apoptosis / drug effects. Benzimidazoles / pharmacology. Blotting, Western. Carcinoma, Medullary / drug therapy. Carcinoma, Medullary / metabolism. Carcinoma, Medullary / secondary. Case-Control Studies. Cell Proliferation / drug effects. Disease Progression. Electrophoretic Mobility Shift Assay. Female. Humans. Immunoenzyme Techniques. Inhibitor of Apoptosis Proteins. Male. Mice. Mice, Inbred BALB C. Mice, Nude. Middle Aged. Peritoneal Neoplasms / drug therapy. Peritoneal Neoplasms / metabolism. Peritoneal Neoplasms / pathology. Receptor, Angiotensin, Type 1 / chemistry. Receptor, ...
in Clinical Cancer Research : An Official Journal of the American Association for Cancer Research (1997), 3(2), 227-31. Tumor samples obtained from 72 patients resected for non-small cell lung cancer were stained immunohistochemically using an immunoperoxidase method and the MLuC5 monoclonal antibody specific for the 67 ... [more ▼]. Tumor samples obtained from 72 patients resected for non-small cell lung cancer were stained immunohistochemically using an immunoperoxidase method and the MLuC5 monoclonal antibody specific for the 67-kDa laminin receptor. Sixty-one of 72 patients (84.7%) displayed a MLuC5-positive reaction, which was usually localized in both the inner surface of the plasmatic membranes and the cytoplasm of neoplastic cells. When we compared the laminin receptor expression with clinicopathological and biological parameters such as histotype, grading, T status, N status, ploidy, proliferative activity, vessel invasion, and p53 protein accumulation, the following results were ...
TY - JOUR. T1 - Fibrous papule. T2 - An immunohistochemical study with an antibody to S-100 protein. AU - Spiegel, Joan. AU - Nadji, Mehrdad. AU - Penneys, Neal S.. PY - 1983/1/1. Y1 - 1983/1/1. N2 - We studied twenty biopsies taken from fibrous papules which were located in the central part of the face. The lesions, which were characteristic histologically, did not contain S-100 protein within the stellate cells in the papillary dermis, nor was this substance found in mesenchymal cells with some features of nevus cells. In control studies, S-100 protein was found using an unlabeled antibody peroxidase-antiperoxidase technic within nevus cells composing junctional, intradermal, and compound nevi, Spitz nevi, and halo nevi. It therefore seems unlikely that fibrous papule represents a form of degenerated nevus as some investigators have proposed.. AB - We studied twenty biopsies taken from fibrous papules which were located in the central part of the face. The lesions, which were characteristic ...
This study aimed at evaluating in outpatients an algorithm for the laboratory diagnosis of Clostridioides (Clostridium) difficile infection (CDI), i.e., enzyme immunoassays (EIAs) detecting bacterial glutamate dehydrogenase (GDH) and toxin A/B, followed by polymerase chain reaction (PCR) analyses of samples with discordant EIA results.. In total, 9802 examinations of stool samples by GDH and toxin EIAs performed in 7263 outpatients and 488 inpatients were analyzed retrospectively. Samples with discordant EIA results had been tested by a commercially available PCR assay detecting genes of the C. difficile-specific triose phosphate isomerase (tpi) and toxin B (tcdB).. Concordant EIA results (686 C. difficile-positive, 8121 negative) were observed for 8807 (89.8%; 95% CI, 89.2-90.4%) samples. Of 958 samples with discordant EIA results, 895 were analyzed using PCR and 580 of 854 GDH-positive/borderline, toxin-negative samples (67.9%; 95% CI, 64.7-71.0%) were positive for tpi and tcdB, while 274 ...
primary intravaginal (IVAG) infection in genetically athymic (nude) mice. Nude (nu/nu) N: NIH(S) and Balb/c mice, as well as their euthymic counterparts were IVAG infected with 5 x 105 pfu of HSV-2. The progression of the infection was followed by HSV-2 immunolabeling using the peroxidase-antiperoxidase technique in tissue sections of the whole body, electron microscopy, and viremia titration at two different timepoints. 70% of athymic NIH mice, 30% of euthymic NIH mice, and 80% of both athymic and euthymic Balb/c mice developed acute vulvovaginitis and died between 8-10 days post-infection (pi). Viremia was not detected in either athymic or euthymic mice. HSV-2 replicated in the vulvovaginal, vesical and perianal epithelia, then progressed towards the central nervous system mainly along autonomic nerves and ganglia. HSV-2 antigens were not detected in liver, spleen, kidney, skin, heart, lung or bone marrow. The conclusion is that the T-cell immune response seems to limit the IVAG infection of ...
We have adapted the PCR-EIA for amplification and detection of an autolysin gene fragment of the pneumococcus. The assay was found to have a quantitative sensitivity for the detection of DNA equivalent to the amount of DNA from three organisms. It detected all tested serotypes of pneumococcus, whereas other streptococci, H. influenzae type b, and S. aureus yielded a negative result.. The qualitative sensitivity and specificity of the assay were estimated on the basis of the results of the assay for the positive controls (specimens for tests of sensitivity) and negative controls (specimens for tests of specificity) and the ability of the assay to detect additional cases of pneumococcal infection determined by the results of the assay with the culture-negative CSF specimens from patients with meningitis. Seven of the eight specimens that were culture or LA positive for pneumococcus were positive by PCR-EIA. The isolate from the only specimen that tested negative was the only one that showed ...
Its February and this patient appears to have influenza. I need a result right now. I know theres a rapid test available.. Anyone involved with infectious disease laboratory testing has heard the above refrain from physicians in the emergency room, intensive care unit, or infectious disease service. Assay manufacturers are constantly developing rapid diagnostic tests for a large variety of infectious diseases. However, an assays speed does not always correlate with an assays performance. Magauran et al,1 in this issue of Infectious Diseases in Clinical Practice, report their experience in using rapid assays for detecting influenza and respiratory syncytial virus (RSV) in their hands during a 2-year time frame. Their laboratory, at the Cleveland Clinic Foundation in Cleveland, Ohio, USA, instituted rapid enzyme immunoassay (EIA) testing for influenza and RSV in the fall of 2004. Specimens that tested negative by the rapid EIA procedures were then subjected to standard direct ...
Report No FR0444 P GALE, B HEGARTY, K WILSON AND C D WATTS APRIL 1994 SUMMARY I BENEFITS An up-to-date review is provided of commercially available kits and new developments for enzyme immunoassay of organics in potable and raw waters enabling Water Industry staff to make informed judgements on the utility of these simple, inexpensive procedures for their monitoring requirements. II OBJECTIVES To review the availability of enzyme immunoassay kits for analysis of pesticides and other substances of concern to the water industry. To evaluate the kits in relation to water industry requirements and conventional analytical methods. To stimulate the development of appropriate kits by discussion of the water industry s requirements with the leading enzyme immunoassay kit manufacturers. To report on the kits developed for the analysis of atrazine and uron pesticides. III REASONS Conventional methods for the analysis of pesticides and other organic chemicals in water often use techniques such as ...
The modified immunoenzymatic method for detection of antibody coated bacteria (IP ACB) was compared with immunofluorescence technique (IF ACB) in the diagnosis of urinary tract infection. For the study 100 patients were employed with significant and insignificant bacteriuria. It was found that 81% of the results obtained by IP ACB and IF ACB were identical, however the immunoenzymatic method was more sensitive than immunofluorescence. Moreover, the IP ACB technique is simpler, less time consuming and may be performed by using the ordinary optic microscope.
The symptoms mentioned above are the mild symptoms for COVID-19, which begins mild and gradually develops into something severe. Some recover from these symptoms without hospitalization. However, others become critically ill and have difficulty in breathing.. The most vulnerable people who can contract the disease are the elderly and people who have underlying medical problems. These medical problems include heart problems, lung problems, diabetes, and cancer. However, those with mild symptoms can transmit the disease to other people.. Coronavirus COVID-19 IgM ELISA Assay Kit. The enzyme-linked immunosorbent assay (ELISA) is a biochemistry assay that detects proteins, peptides, hormones, and antigens from test samples. Detection is achieved by assessing the conjugated enzyme activity. The most crucial element in detection is the antigen-antibody interaction. ELISA assay uses the microplate-based enzyme immunoassay technique.. The pathogenic strains developed by coronaviruses cause respiratory ...
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A single human tumor tissue with 5-10 m thickness is mounted on a positively charged glass slide. The slides are fixed and dehydrated with acetone for consistent results with in situ hybridization and immunohistochemistry. More tumors maybe available upon request ...
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The ViraChip® is an immunoblot based on an enzyme-immunoassay in a microarray format, carrying highly purified specific antigens bound to nitrocellulose at defined positions. It is suited for the qualitative detection of antibodies against specific antigens in human serum.. One microarray is fixed at the bottom of each well in a standard microtiter plate (MTP). The single breakable wells are stored in a holding frame with 96 positions. During the serum incubation step antigen-specific antibodies bind to the immobilised antigens, herein after referred to as spots, on the microarray. During the conjugate reaction, the AP-conjugate binds to the antigen-antibody complex. The alkaline phosphatase converts the chromogen/substrate and thus, stains the antigen-antibody complex on the microarray purple. The washing procedures following serum, conjugate and chromogen/substrate incubation steps remove unbound antibodies and reagents.. The control spots include serum controls, conjugate controls, ...
This antibody is designed to detect laminin using western blotting analysis under non-reducing and non-heating conditions and for histology on frozen tissue sections and paraffin embedded tissue sections.
This antibody is designed to detect laminin using western blotting analysis under non-reducing and non-heating conditions and for histology on frozen tissue sections and paraffin embedded tissue sections.
Author(s): Stern, Kalyn Michiko | Abstract: Drug sensitization is thought to arise through changes in transcription, modification of signaling, and synaptic transmission. A novel rat gene, mrt1, may contribute to this neuronal plasticity as it is upregulated during sensitization. Mrt1 contains a PX domain which classifies it as a sorting nexins- thus mrt1 is also called SNX27. In addition, SNX27 also has a PDZ domain which has been demonstrated to interact with and mediate GIRK channel trafficking. To explore this interaction and the role that SNX27 may play in sensitization it is necessary to localize SNX27 expression. Using a novel SNX27 antibody I had three aims for my Masters project. First, to characterize the specificity of the antibody. Second, to characterize SNX27 expression in the brain, particularly in the hippocampus. Third, to correlate this expression back to an interaction with GIRK channels. The SNX27 antibody demonstrated good specificity for SNX27 protein. SNX27 was found to be
Principal Investigator:KATO Ihachi, Project Period (FY):1996 - 1997, Research Category:Grant-in-Aid for Scientific Research (B), Section:一般, Research Field:Conservative dentistry
The unlabeled antibody enzyme strategy of immunohistochemistry: preparation and properties of soluble antigen-antibody sophisticated (horseradish peroxidase-antihorseradish peroxidase) and its use in identification of spirochetes. Interleukin-10 (IL-10) impacts the growth and differentiation of many hemopoietic cells in vitro; notably, it is a potent suppressor of macrophage and T cell capabilities. In IL-10-deficient mice, generated by gene specializing in, […]. ...
An Autumn Peach Reviewing my past year with uterine (endometrial) cancer, I am grateful for and thank everyone for their support. Also wishing my sisters with all gynecologic and breast cancers the very best this fall season. October 10, 2013: A year ago today, my reading group met to discuss The Book Thief by Markus Zusak,…
Learn more about Radiation Therapy for Uterine (Endometrial) Cancer at Sky Ridge Medical Center Main Page Risk Factors ...
Learn more about Surgical Procedures for Uterine (Endometrial) Cancer at Sky Ridge Medical Center Main Page Risk Factors ...
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