A technique that combines protein electrophoresis and double immunodiffusion. In this procedure proteins are first separated by gel electrophoresis (usually agarose), then made visible by immunodiffusion of specific antibodies. A distinct elliptical precipitin arc results for each protein detectable by the antisera.
Immunoelectrophoresis in which a second electrophoretic transport is performed on the initially separated antigen fragments into an antibody-containing medium in a direction perpendicular to the first electrophoresis.
Immunoelectrophoresis in which immunoprecipitation occurs when antigen at the cathode is caused to migrate in an electric field through a suitable medium of diffusion against a stream of antibody migrating from the anode as a result of endosmotic flow.
Technique involving the diffusion of antigen or antibody through a semisolid medium, usually agar or agarose gel, with the result being a precipitin reaction.
Serum that contains antibodies. It is obtained from an animal that has been immunized either by ANTIGEN injection or infection with microorganisms containing the antigen.
Electrophoresis applied to BLOOD PROTEINS.
Substances elaborated by bacteria that have antigenic activity.
Antibodies which elicit IMMUNOPRECIPITATION when combined with antigen.
A sterile solution containing the by-products of growth products of COCCIDIOIDES IMMITIS, injected intracutaneously as a test for COCCIDIOIDOMYCOSIS.
Serological reactions in which an antiserum against one antigen reacts with a non-identical but closely related antigen.
Substances that are recognized by the immune system and induce an immune reaction.
The sum of the weight of all the atoms in a molecule.
Electrophoresis in which a polyacrylamide gel is used as the diffusion medium.
Proteins that are present in blood serum, including SERUM ALBUMIN; BLOOD COAGULATION FACTORS; and many other types of proteins.
Pregnane derivatives containing two double bonds anywhere within the ring structures.
The species Oryctolagus cuniculus, in the family Leporidae, order LAGOMORPHA. Rabbits are born in burrows, furless, and with eyes and ears closed. In contrast with HARES, rabbits have 22 chromosome pairs.
Field of chemistry that pertains to immunological phenomena and the study of chemical reactions related to antigen stimulation of tissues. It includes physicochemical interactions between antigens and antibodies.
Serum globulins with high molecular weight. (Dorland, 28th ed)
Electrophoresis in which agar or agarose gel is used as the diffusion medium.
The formation of a solid in a solution as a result of a chemical reaction or the aggregation of soluble substances into complexes large enough to fall out of solution.
Chromatography on non-ionic gels without regard to the mechanism of solute discrimination.
Electrophoresis in which a pH gradient is established in a gel medium and proteins migrate until they reach the site (or focus) at which the pH is equal to their isoelectric point.
Substances of fungal origin that have antigenic activity.
A mitosporic fungal genus which causes COCCIDIOIDOMYCOSIS.
Electrophoresis in which cellulose acetate is the diffusion medium.
Polysaccharides found in bacteria and in capsules thereof.
The largest class of organic compounds, including STARCH; GLYCOGEN; CELLULOSE; POLYSACCHARIDES; and simple MONOSACCHARIDES. Carbohydrates are composed of carbon, hydrogen, and oxygen in a ratio of Cn(H2O)n.
Techniques for removal by adsorption and subsequent elution of a specific antibody or antigen using an immunosorbent containing the homologous antigen or antibody.
The complex formed by the binding of antigen and antibody molecules. The deposition of large antigen-antibody complexes leading to tissue damage causes IMMUNE COMPLEX DISEASES.
The processes triggered by interactions of ANTIBODIES with their ANTIGENS.
A group of related diseases characterized by an unbalanced or disproportionate proliferation of immunoglobulin-producing cells, usually from a single clone. These cells frequently secrete a structurally homogeneous immunoglobulin (M-component) and/or an abnormal immunoglobulin.
Serum globulins that migrate to the gamma region (most positively charged) upon ELECTROPHORESIS. At one time, gamma-globulins came to be used as a synonym for immunoglobulins since most immunoglobulins are gamma globulins and conversely most gamma globulins are immunoglobulins. But since some immunoglobulins exhibit an alpha or beta electrophoretic mobility, that usage is in decline.
An electrochemical process in which macromolecules or colloidal particles with a net electric charge migrate in a solution under the influence of an electric current.
A strong acid used as a protein precipitant in clinical chemistry and also as a caustic for removing warts.
Sensitive tests to measure certain antigens, antibodies, or viruses, using their ability to agglutinate certain erythrocytes. (From Stedman, 26th ed)
Tests that are dependent on the clumping of cells, microorganisms, or particles when mixed with specific antiserum. (From Stedman, 26th ed)
A synthetic steroid with antigonadotropic and anti-estrogenic activities that acts as an anterior pituitary suppressant by inhibiting the pituitary output of gonadotropins. It possesses some androgenic properties. Danazol has been used in the treatment of endometriosis and some benign breast disorders.
Serum proteins with an electrophoretic mobility that falls between ALPHA-GLOBULINS and GAMMA-GLOBULINS.
Techniques used to demonstrate or measure an immune response, and to identify or measure antigens using antibodies.
Sites on an antigen that interact with specific antibodies.
Centrifugation with a centrifuge that develops centrifugal fields of more than 100,000 times gravity. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)
Abnormal immunoglobulins characteristic of MULTIPLE MYELOMA.
An organism originally isolated from sewage, manure, humus, and soil, but recently found as a parasite in mammals and birds.
Organic compounds that generally contain an amino (-NH2) and a carboxyl (-COOH) group. Twenty alpha-amino acids are the subunits which are polymerized to form proteins.
Electrophoresis in which discontinuities in both the voltage and pH gradients are introduced by using buffers of different composition and pH in the different parts of the gel column. The term 'disc' was originally used as an abbreviation for 'discontinuous' referring to the buffers employed, and does not have anything to do with the shape of the separated zones.
The etiologic agent of murine typhus (see TYPHUS, ENDEMIC FLEA-BORNE).
Absence or reduced levels of PROTHROMBIN in the blood.
Serologic tests in which a positive reaction manifested by visible CHEMICAL PRECIPITATION occurs when a soluble ANTIGEN reacts with its precipitins, i.e., ANTIBODIES that can form a precipitate.
All blood proteins except albumin ( = SERUM ALBUMIN, which is not a globulin) and FIBRINOGEN (which is not in the serum). The serum globulins are subdivided into ALPHA-GLOBULINS; BETA-GLOBULINS; and GAMMA-GLOBULINS on the basis of their electrophoretic mobilities. (From Dorland, 28th ed)
The pH in solutions of proteins and related compounds at which the dipolar ions are at a maximum.
The major immunoglobulin isotype class in normal human serum. There are several isotype subclasses of IgG, for example, IgG1, IgG2A, and IgG2B.
Abnormal immunoglobulins synthesized by atypical cells of the MONONUCLEAR PHAGOCYTE SYSTEM. Paraproteins containing only light chains lead to Bence Jones paraproteinemia, while the presence of only atypical heavy chains leads to heavy chain disease. Most of the paraproteins show themselves as an M-component (monoclonal gammopathy) in electrophoresis. Diclonal and polyclonal paraproteins are much less frequently encountered.
DYSENTERY caused by intestinal amebic infection, chiefly with ENTAMOEBA HISTOLYTICA. This condition may be associated with amebic infection of the LIVER and other distant sites.
Conjugated protein-carbohydrate compounds including mucins, mucoid, and amyloid glycoproteins.
The restriction of a characteristic behavior, anatomical structure or physical system, such as immune response; metabolic response, or gene or gene variant to the members of one species. It refers to that property which differentiates one species from another but it is also used for phylogenetic levels higher or lower than the species.
A series of steps taken in order to conduct research.
An abnormal protein with unusual thermosolubility characteristics that is found in the urine of patients with MULTIPLE MYELOMA.
Serologic tests based on inactivation of complement by the antigen-antibody complex (stage 1). Binding of free complement can be visualized by addition of a second antigen-antibody system such as red cells and appropriate red cell antibody (hemolysin) requiring complement for its completion (stage 2). Failure of the red cells to lyse indicates that a specific antigen-antibody reaction has taken place in stage 1. If red cells lyse, free complement is present indicating no antigen-antibody reaction occurred in stage 1.
A genus of gram-positive, rod-shaped bacteria whose organisms are nonmotile. Filaments that may be present in certain species are either straight or wavy and may have swollen or clubbed heads.
An excess of GAMMA-GLOBULINS in the serum due to chronic infections or PARAPROTEINEMIAS.
Techniques used to separate mixtures of substances based on differences in the relative affinities of the substances for mobile and stationary phases. A mobile phase (fluid or gas) passes through a column containing a stationary phase of porous solid or liquid coated on a solid support. Usage is both analytical for small amounts and preparative for bulk amounts.
Separation technique in which the stationary phase consists of ion exchange resins. The resins contain loosely held small ions that easily exchange places with other small ions of like charge present in solutions washed over the resins.
One of the types of light chains of the immunoglobulins with a molecular weight of approximately 22 kDa.
Laboratory tests for evaluating the individual's clotting mechanism.
A chromatographic technique that utilizes the ability of biological molecules to bind to certain ligands specifically and reversibly. It is used in protein biochemistry. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)
One of the types of light chain subunits of the immunoglobulins with a molecular weight of approximately 22 kDa.
Inflammation of the coverings of the brain and/or spinal cord, which consist of the PIA MATER; ARACHNOID; and DURA MATER. Infections (viral, bacterial, and fungal) are the most common causes of this condition, but subarachnoid hemorrhage (HEMORRHAGES, SUBARACHNOID), chemical irritation (chemical MENINGITIS), granulomatous conditions, neoplastic conditions (CARCINOMATOUS MENINGITIS), and other inflammatory conditions may produce this syndrome. (From Joynt, Clinical Neurology, 1994, Ch24, p6)
A synthetic steroid that has anabolic and androgenic properties. (From Martindale, The Extra Pharmacopoeia, 30th ed, p1194)
Antigens on surfaces of cells, including infectious or foreign cells or viruses. They are usually protein-containing groups on cell membranes or walls and may be isolated.
An absence or reduced level of Antithrombin III leading to an increased risk for thrombosis.
Represents 15-20% of the human serum immunoglobulins, mostly as the 4-chain polymer in humans or dimer in other mammals. Secretory IgA (IMMUNOGLOBULIN A, SECRETORY) is the main immunoglobulin in secretions.
A basic science concerned with the composition, structure, and properties of matter; and the reactions that occur between substances and the associated energy exchange.
The property of antibodies which enables them to react with some ANTIGENIC DETERMINANTS and not with others. Specificity is dependent on chemical composition, physical forces, and molecular structure at the binding site.
The composition, conformation, and properties of atoms and molecules, and their reaction and interaction processes.
A major protein in the BLOOD. It is important in maintaining the colloidal osmotic pressure and transporting large organic molecules.
Purifying or cleansing agents, usually salts of long-chain aliphatic bases or acids, that exert cleansing (oil-dissolving) and antimicrobial effects through a surface action that depends on possessing both hydrophilic and hydrophobic properties.
A glycoprotein that is central in both the classical and the alternative pathway of COMPLEMENT ACTIVATION. C3 can be cleaved into COMPLEMENT C3A and COMPLEMENT C3B, spontaneously at low level or by C3 CONVERTASE at high level. The smaller fragment C3a is an ANAPHYLATOXIN and mediator of local inflammatory process. The larger fragment C3b binds with C3 convertase to form C5 convertase.
Non-nucleated disk-shaped cells formed in the megakaryocyte and found in the blood of all mammals. They are mainly involved in blood coagulation.
Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
A glycoprotein that is important in the activation of CLASSICAL COMPLEMENT PATHWAY. C4 is cleaved by the activated COMPLEMENT C1S into COMPLEMENT C4A and COMPLEMENT C4B.
Multi-subunit proteins which function in IMMUNITY. They are produced by B LYMPHOCYTES from the IMMUNOGLOBULIN GENES. They are comprised of two heavy (IMMUNOGLOBULIN HEAVY CHAINS) and two light chains (IMMUNOGLOBULIN LIGHT CHAINS) with additional ancillary polypeptide chains depending on their isoforms. The variety of isoforms include monomeric or polymeric forms, and transmembrane forms (B-CELL ANTIGEN RECEPTORS) or secreted forms (ANTIBODIES). They are divided by the amino acid sequence of their heavy chains into five classes (IMMUNOGLOBULIN A; IMMUNOGLOBULIN D; IMMUNOGLOBULIN E; IMMUNOGLOBULIN G; IMMUNOGLOBULIN M) and various subclasses.
A technique using antibodies for identifying or quantifying a substance. Usually the substance being studied serves as antigen both in antibody production and in measurement of antibody by the test substance.
Plasma glycoprotein member of the serpin superfamily which inhibits TRYPSIN; NEUTROPHIL ELASTASE; and other PROTEOLYTIC ENZYMES.
Proteins found in any species of bacterium.
Process of determining and distinguishing species of bacteria or viruses based on antigens they share.
The clumping together of suspended material resulting from the action of AGGLUTININS.
The lipid- and protein-containing, selectively permeable membrane that surrounds the cytoplasm in prokaryotic and eukaryotic cells.
Glycoproteins with a molecular weight of approximately 620,000 to 680,000. Precipitation by electrophoresis is in the alpha region. They include alpha 1-macroglobulins and alpha 2-macroglobulins. These proteins exhibit trypsin-, chymotrypsin-, thrombin-, and plasmin-binding activity and function as hormonal transporters.
Polypeptide chains, consisting of 211 to 217 amino acid residues and having a molecular weight of approximately 22 kDa. There are two major types of light chains, kappa and lambda. Two Ig light chains and two Ig heavy chains (IMMUNOGLOBULIN HEAVY CHAINS) make one immunoglobulin molecule.
Immunoglobulins produced in a response to BACTERIAL ANTIGENS.
Disorders caused by abnormalities in platelet count or function.
A type of ion exchange chromatography using diethylaminoethyl cellulose (DEAE-CELLULOSE) as a positively charged resin. (From McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)
A methylpentose whose L- isomer is found naturally in many plant glycosides and some gram-negative bacterial lipopolysaccharides.
Immunoglobulin molecules having a specific amino acid sequence by virtue of which they interact only with the ANTIGEN (or a very similar shape) that induced their synthesis in cells of the lymphoid series (especially PLASMA CELLS).
A genus of gram-positive, coccoid bacteria whose organisms occur in pairs or chains. No endospores are produced. Many species exist as commensals or parasites on man or animals with some being highly pathogenic. A few species are saprophytes and occur in the natural environment.
A plasma alpha 2 glycoprotein that accounts for the major antithrombin activity of normal plasma and also inhibits several other enzymes. It is a member of the serpin superfamily.
An iron-binding beta1-globulin that is synthesized in the LIVER and secreted into the blood. It plays a central role in the transport of IRON throughout the circulation. A variety of transferrin isoforms exist in humans, including some that are considered markers for specific disease states.
Classic quantitative assay for detection of antigen-antibody reactions using a radioactively labeled substance (radioligand) either directly or indirectly to measure the binding of the unlabeled substance to a specific antibody or other receptor system. Non-immunogenic substances (e.g., haptens) can be measured if coupled to larger carrier proteins (e.g., bovine gamma-globulin or human serum albumin) capable of inducing antibody formation.
Serum proteins that have the most rapid migration during ELECTROPHORESIS. This subgroup of globulins is divided into faster and slower alpha(1)- and alpha(2)-globulins.
Surface glycoproteins on platelets which have a key role in hemostasis and thrombosis such as platelet adhesion and aggregation. Many of these are receptors.
The outermost layer of a cell in most PLANTS; BACTERIA; FUNGI; and ALGAE. The cell wall is usually a rigid structure that lies external to the CELL MEMBRANE, and provides a protective barrier against physical or chemical agents.
The physical or physiological processes by which substances, tissue, cells, etc. take up or take in other substances or energy.
Passive agglutination tests in which antigen is adsorbed onto latex particles which then clump in the presence of antibody specific for the adsorbed antigen. (From Stedman, 26th ed)
A serine endopeptidase that is formed from TRYPSINOGEN in the pancreas. It is converted into its active form by ENTEROPEPTIDASE in the small intestine. It catalyzes hydrolysis of the carboxyl group of either arginine or lysine. EC 3.4.21.4.
A complex sulfated polymer of galactose units, extracted from Gelidium cartilagineum, Gracilaria confervoides, and related red algae. It is used as a gel in the preparation of solid culture media for microorganisms, as a bulk laxative, in making emulsions, and as a supporting medium for immunodiffusion and immunoelectrophoresis.
Endogenous substances, usually proteins, that are involved in the blood coagulation process.
The aggregation of ERYTHROCYTES by AGGLUTININS, including antibodies, lectins, and viral proteins (HEMAGGLUTINATION, VIRAL).
A class of immunoglobulin bearing mu chains (IMMUNOGLOBULIN MU-CHAINS). IgM can fix COMPLEMENT. The name comes from its high molecular weight and originally being called a macroglobulin.
A high-molecular-weight plasma protein, produced by endothelial cells and megakaryocytes, that is part of the factor VIII/von Willebrand factor complex. The von Willebrand factor has receptors for collagen, platelets, and ristocetin activity as well as the immunologically distinct antigenic determinants. It functions in adhesion of platelets to collagen and hemostatic plug formation. The prolonged bleeding time in VON WILLEBRAND DISEASES is due to the deficiency of this factor.
Compounds and molecular complexes that consist of very large numbers of atoms and are generally over 500 kDa in size. In biological systems macromolecular substances usually can be visualized using ELECTRON MICROSCOPY and are distinguished from ORGANELLES by the lack of a membrane structure.
Hydrolases that specifically cleave the peptide bonds found in PROTEINS and PEPTIDES. Examples of sub-subclasses for this group include EXOPEPTIDASES and ENDOPEPTIDASES.
An immunoassay utilizing an antibody labeled with an enzyme marker such as horseradish peroxidase. While either the enzyme or the antibody is bound to an immunosorbent substrate, they both retain their biologic activity; the change in enzyme activity as a result of the enzyme-antibody-antigen reaction is proportional to the concentration of the antigen and can be measured spectrophotometrically or with the naked eye. Many variations of the method have been developed.
Proteins which are found in membranes including cellular and intracellular membranes. They consist of two types, peripheral and integral proteins. They include most membrane-associated enzymes, antigenic proteins, transport proteins, and drug, hormone, and lectin receptors.
Plasma glycoprotein clotted by thrombin, composed of a dimer of three non-identical pairs of polypeptide chains (alpha, beta, gamma) held together by disulfide bonds. Fibrinogen clotting is a sol-gel change involving complex molecular arrangements: whereas fibrinogen is cleaved by thrombin to form polypeptides A and B, the proteolytic action of other enzymes yields different fibrinogen degradation products.
Polymers of ETHYLENE OXIDE and water, and their ethers. They vary in consistency from liquid to solid depending on the molecular weight indicated by a number following the name. They are used as SURFACTANTS, dispersing agents, solvents, ointment and suppository bases, vehicles, and tablet excipients. Some specific groups are NONOXYNOLS, OCTOXYNOLS, and POLOXAMERS.
Group of hemorrhagic disorders in which the VON WILLEBRAND FACTOR is either quantitatively or qualitatively abnormal. They are usually inherited as an autosomal dominant trait though rare kindreds are autosomal recessive. Symptoms vary depending on severity and disease type but may include prolonged bleeding time, deficiency of factor VIII, and impaired platelet adhesion.
The ability of a substance to be dissolved, i.e. to form a solution with another substance. (From McGraw-Hill Dictionary of Scientific and Technical Terms, 6th ed)
A species of gram-negative, facultatively anaerobic, rod-shaped bacteria (GRAM-NEGATIVE FACULTATIVELY ANAEROBIC RODS) commonly found in the lower part of the intestine of warm-blooded animals. It is usually nonpathogenic, but some strains are known to produce DIARRHEA and pyogenic infections. Pathogenic strains (virotypes) are classified by their specific pathogenic mechanisms such as toxins (ENTEROTOXIGENIC ESCHERICHIA COLI), etc.
A malignancy of mature PLASMA CELLS engaging in monoclonal immunoglobulin production. It is characterized by hyperglobulinemia, excess Bence-Jones proteins (free monoclonal IMMUNOGLOBULIN LIGHT CHAINS) in the urine, skeletal destruction, bone pain, and fractures. Other features include ANEMIA; HYPERCALCEMIA; and RENAL INSUFFICIENCY.
Blood-coagulation factor VIII. Antihemophilic factor that is part of the factor VIII/von Willebrand factor complex. Factor VIII is produced in the liver and acts in the intrinsic pathway of blood coagulation. It serves as a cofactor in factor X activation and this action is markedly enhanced by small amounts of thrombin.
A large lobed glandular organ in the abdomen of vertebrates that is responsible for detoxification, metabolism, synthesis and storage of various substances.
Presence of warmth or heat or a temperature notably higher than an accustomed norm.
An anionic surfactant, usually a mixture of sodium alkyl sulfates, mainly the lauryl; lowers surface tension of aqueous solutions; used as fat emulsifier, wetting agent, detergent in cosmetics, pharmaceuticals and toothpastes; also as research tool in protein biochemistry.
Bacterial polysaccharides that are rich in phosphodiester linkages. They are the major components of the cell walls and membranes of many bacteria.
A genus of gram-positive, aerobic bacteria. Most species are free-living in soil and water, but the major habitat for some is the diseased tissue of warm-blooded hosts.
A polysaccharide-producing species of STREPTOCOCCUS isolated from human dental plaque.
Any of numerous agile, hollow-horned RUMINANTS of the genus Capra, in the family Bovidae, closely related to the SHEEP.
Microscopy using an electron beam, instead of light, to visualize the sample, thereby allowing much greater magnification. The interactions of ELECTRONS with specimens are used to provide information about the fine structure of that specimen. In TRANSMISSION ELECTRON MICROSCOPY the reactions of the electrons that are transmitted through the specimen are imaged. In SCANNING ELECTRON MICROSCOPY an electron beam falls at a non-normal angle on the specimen and the image is derived from the reactions occurring above the plane of the specimen.
A class of lipoproteins of small size (4-13 nm) and dense (greater than 1.063 g/ml) particles. HDL lipoproteins, synthesized in the liver without a lipid core, accumulate cholesterol esters from peripheral tissues and transport them to the liver for re-utilization or elimination from the body (the reverse cholesterol transport). Their major protein component is APOLIPOPROTEIN A-I. HDL also shuttle APOLIPOPROTEINS C and APOLIPOPROTEINS E to and from triglyceride-rich lipoproteins during their catabolism. HDL plasma level has been inversely correlated with the risk of cardiovascular diseases.
Serum glycoproteins participating in the host defense mechanism of COMPLEMENT ACTIVATION that creates the COMPLEMENT MEMBRANE ATTACK COMPLEX. Included are glycoproteins in the various pathways of complement activation (CLASSICAL COMPLEMENT PATHWAY; ALTERNATIVE COMPLEMENT PATHWAY; and LECTIN COMPLEMENT PATHWAY).

Homogeneous pyruvate kinase isolated from yeast by two different methods is indistinguishable from pyruvate kinase in cell-free extract. (1/2055)

In this report, we have compared homogeneous yeast (Saccharomyces cerevisiae) pyruvate kinase to enzyme from cell-free extracts in several different ways: 1) isoelectric focusing of cell-free extracts indicates one peak of pyruvate kinase activity whose isoelectric point is the same as that of the pure enzyme; 2) antibody prepared to the pure enzyme produces a single, fused precipitin line against enzyme in the cell-free extract and pure enzyme; 3) immunoelectrophoresis of cell-free extract produces one precipitin arc which has the same mobility as that of the pure enzyme; and 4) immunoprecipitation of the pure enzyme from cell-free extract with subsequent solubilization in 1% sodium dodecyl sulfate and electrophoresis on sodium dodecyl sulfate-polyacrylamide gels produces a single protein band attributable to pyruvate kinase which co-migrates with the purified enzyme. Within the limits of the sensitivity of the methods employed, we conclude that the homogeneous pyruvate kinase prepared from yeast lysed either by Manton-Gaulin homogenization (Aust, A., Yun, S.-L., and Suelter, C. (1975) Methods Enzymol. 42, 176-182) or by toluolysis (Yun, S.-L., Aust, A.E., and Suelter, C.H. (1977) J. Biol. Chem. 251, 124-128) is identical with pyruvate kinase in cell-free extract.  (+info)

Enzymatic and immunological characterization of the Mycobacterium fortuitum complex. (2/2055)

The arylsulfatase isozymes of Mycobacterium fortuitum, M. peregrinum, M. chelonei subsp. chelonei, and M. chelonei subsp. abscessus were examined to determine the isozymal and immunological relationship among the members of the M. fortuitum complex. Cell extracts were subjected to electrophoresis on agarose and polyacrylamide gel, and arylsulfatase activity was localized using beta-naphthyl sulfate as substrate. Unique zymograms were produced for M. fortuitum, M. peregrinum, and M. chelonei which were characteristic for each species. The immunological relationship among the sulfatases was assayed by using immunodiffusion and immunoelectrophoresis followed by sulfatase staining for the enzyme. One of the isozymes of M. fortuitum and M. peregrinum cross-reacted, showing immunological identity. Antisera to sulfatases of M. fortuitum and M. peregrinum did not react with sulfatases of M. chelonei. The characterization of sulfatase isozymes in extracts of organisms in the M. fortuitum complex suggests the division of the M. fortuitum complex into two species, M. fortuitum and M. chelonei, with subspecies designations.  (+info)

The phenoloxidases of the ascomycete Podospora anserina. Structural differences between laccases of high and low molecular weight. (3/2055)

In order to investigate the extent of the relationship between the three copper-containing glycoproteins, laccases I, II and III (Mr70000, 80000 and 390000 respectively) of Podospora anserina, the following experiments were carried out on laccases II and III: (a) determination of amino acid composition; (b) determination of N-terminal and C-terminal amino acid; (c) determination of sugar composition; (d) dissociation studies on native and denatured laccases and also after removal of copper from the enzymes; (e) digestion of the carbohydrate moieties with the aid of glycosylhydrolases. A comparison between the results of these experiments and data previously obtained with laccase I allows the following conclusions to be drawn. 1. Laccases II and III are not identical. 2. Neither of these low molecular weight laccases are as complete molecules subunits of the oligomeric laccase I. 3. The possibility of partial identity of amino acid sequences of laccases I and III can not be excluded. 4. Laccase II possibly consists of subunits of Mr37000 whereas laccase III does not. 5. Digestion of 50% of the carbohydrate content leads to complete loss of serological specificity (serological reaction and cross reaction). This finding is discussed with regard to the possible role of the carbohydrate moiety as antigenic determinants and thus as the reason for the immunological relationship. As a consequence, at least three independent structural genes for laccases must be assumed.  (+info)

Inhibition of human seminal fluid DNA polymerase by an IgG fraction of seminal plasma from vasectomized men. (4/2055)

Immunoglobulin G (IgG) was isolated from ejaculates of intact and vasectomized men by precipitation with ammonium sulphate and DEAE-cellulose ionexchange chromatography. Velocity centrifugation revealed that all of the IgG from intact males was 7S protein while less than 40% of the seminal IgG of vasectomized men cosedimented with the 7S marker; the remaining, immunologically unidentifiable, protein was considerably smaller and heterogeneous in size. Only the 7S IgG from the post-vasectomy ejaculates inhibited the activity of a DNA polymerase from the seminal fluid of an intact male. These results suggest that formation of antibody reactive with the seminal fluid DNA polymerase is one manifestation of a vasectomy-associated autoimmune response in man.  (+info)

Complement activity in middle ear effusions. (5/2055)

Evidence for complement utilization in middle ear fluids (MEF) from patients with otitis media with effusion was sought. It was found that cleavage products of C3, C4 and Factor B could be demonstrated immunochemically in MEF, and that native C3 was present in much lower concentrations than other proteins, relative to their serum concentrations. Haemolytic assays for C1-C5 showed that early complement components are inactivated in MEF. Potential mechanisms for complement utilization in MEF are discussed.  (+info)

Enterobacterial common antigen: isolation from Shigella sonnei, purification and immunochemical characterization. (6/2055)

In the studies presented the effective procedure of isolation and purification of enterobacterial common antigen from Shigella sonnei has been elaborated. The method is based on sonification of bacterial suspension in the presence of lysozyme and EDTA and subsequent extraction of the pellet with boiling water. The crude extract of common antigen was purified by fractionation with ethanol and chromatography on silica gel and Sephadex LH-20. The comparison of several extraction procedures of enterobacterial common antigen from Shigella sonnei proved that the method described above is most effective. The purified enterobacterial common antigen preparation obtained preserved full biological activity: antigenicity (precipitation and activity in enzyme-linked immunosorbent assay), immunogenicity in rabbits, ability to coat erythrocytes (passive hemagglutination) and inhibitory activity in passive hemagglutination. The pure enterobacterial common antigen was identified to 90% as a polymer of N-acetyl-D-mannosaminuronic acid and N-acetyl-D-glucosamine (2:1, molar ratio), O-acetylated and containing 3.2% fatty acids (C16:0 and C18:1, not oleic). It contains 5.3% nitrogen, less than 4% protein, less than 0.5% phosphorus and less than 1.6% neutral sugar; glycerol and RNA were not found in the preparation.  (+info)

Immune response in the garter snake (Thamnophis ordinoides). (7/2055)

Garter snakes (Thamnophis ordinoides) were immunized with hen egg albumin, human gamma-globulin and Keyhole limpet haemocyanin in Freund's adjuvant. Antibody was consistently detected by radioimmunoelectrophoresis and in three different gamma- and beta-globulin precipitin lines called IgM (approximately or equal to 20S), Ig-1 (approximately or equal to 9S) and Ig-2 (approximately or equal to 8-5S). Early antibody (day 31 after immunization) was frequently Ig-M whereas Ig-2 and especially Ig-1 were detectable for the longest duration (992 days). After immunization with antigen in Freund's adjuvant, Ig-1 serum concentration showed the greatest increase, from almost undetectable levels to the most prominent immunoglobulin in immune serum.  (+info)

Plant microbody proteins. Purification and glycoprotein nature of glyoxysomal isocitrate lyase from cucumber cotyledons. (8/2055)

1. Isocitrate lyase from cotyledons of cucumber seedlings (Cucumis sativus) has been purified 100-fold. Two methods of preparing the soluble glyoxylate cycle enzyme are described: an elaborated method which used crude extracts of cucumber cotyledons, and another procedure which started with purified glyoxysomes from 4-day-old cotyledons and included a separation of glyoxysomal matrix enzymes by zonal centrifugation. The product behaved as a single species when tested by (a) polyacrylamide gel electrophoresis in the presence of dodecyl sulfate, (b) zonal centrifugation, and (c) double immunodiffusion against rabbit antibody to isocitrate lyase. 2. Isocitrate lyase of cucumber glyoxysomes exhibited a molecular weight of 255,000 and was composed of four apparently identical subunits of Mr 64,000. An isoelectric point of 5.9 was determined. 3. It was shown that isocitrate lyase is a glycoprotein, (a) by Schiff stain on polyacrylamide gels, (b) by periodate oxidation of the enzyme, subsequent reduction with NaB[3H]4 and electrophoretic analysis of the labelled glycoprotein, and (c) by incorporation of [3H]glucosamine in vivo into a protein which could be precipitated with antibodies to isocitrate lyase and revealed a 64,000-Mr band upon electrophoresis.  (+info)

1. Experimental principle Rocket immunoelectrophoresis (RIEP) is a quantitative detection technique that combines unidirectional immunodiffusion and electrophoresis. During electrophoresis, the antibody contained in the agar gel does not move, but the antigen in the sample is caused to swim toward the positive electrode under the action of the electric field. When the antigen and antibody molecules reach the appropriate ratio, a peak of insoluble immune complex shaped like a rocket is formed, and the height of the peak is positively correlated with the antigen concentration in the sample collection. Therefore, when the antibody concentration in the agar is fixed, the standard curve is drawn with the precipitation peak formed after the standard antigens of different dilutions swimming and the antigen concentration as the abscissa. The content of the antigen to be tested can be calculated according to the length of the precipitation peak of the sample; conversely, when the concentration of the ...
Guinea pig 7S immunoglobulins (γ1 and γ2) consist of two groups of molecules (K and L) bearing different types of L chains (κ and λ). Approximately one-third of
Looking for immunoelectrophoresis? Find out information about immunoelectrophoresis. A combination of the techniques of electrophoresis and immunodiffusion used to separate the components of a mixture of antigens and make them visible by... Explanation of immunoelectrophoresis
The WS-1B rocket, with a new high-performance rocket motor and warhead, reaches a maximum speed of Mach 5.2 and maximum flight altitude of 60km, giving a minimum firing range of 60km and a maximum firing range of 180km. The probability deviation is between 1% and 1.25%. The WS-1B rocket is longer than the WS-1, with length 6.37m but the same diameter. The take-off weight is 725kg with a 150kg warhead.. The free rocket consists of the warhead and fuse, an FG-43 rocket motor and the tail section. The FG-43 rocket motor is a single chamber, solid rocket motor with an advanced hydroxy-terminated polybutadine (HTPB) composition rocket propellant.. Two types of warhead can be fitted on the WS-IB rocket, a ZDB-2 blasting warhead or an SZB-1 submunition, which are selected according to the characteristics of the target.. The ZDB-2 blasting warhead is loaded with steel balls and prefabricated fragments. The SZB-1 submunition warhead provides an effective high power weapon against massed tanks. When the ...
National Documentation Centre (EKT). National Archive of PhD Theses.PhD thesis.1986 . Creators: ΚΩΤΣΟΒΑΣΙΛΗΣ, ΚΩΝΣΤΑΝΤΙΝΟΣ.IN THE PRESENT STUDY THE DEGREE OF GLYCATION OF A1-ACID GLYCOPROTEIN (A1AGP) IN MALIGNANCY WAS INVESTIGATED BY THE METHOD OF AFFINITY IMMUNE ELECTROPHORESISIN TWO DIMENSIONS WITH CONCANAVALIN A IN THE FIRST DIMENSION. THE IMMUNOELECTROPHORETIC PATTERN OF A1AGP IN THE SERA FROM PATIENTS WITH VARIOUS NEOPLASTIC DISORDERS WAS INVESTIGATED TO DETERMINE WHETHER THE DEGREE OF GLYCATION OF A1AGP IS AFFECTED IN THE VARIOUS MALIGNANT GROUPS AND TO WHAT EXTENT THE CHANGES OBSERVED ARE CONSISTENT WITHIN A TUMOR GROUP AND CAN BE CHARACTERISTIC OF THE GROUP. THE TUMORS STUDIED WERE CLINICALLY CLASSIFIED INTO 11 GROUPS IN WHICH 8 DIFFERENT TYPES OF CHARACTERISTIC GLYCATION PATTERNS OF A1AGP HAVE BEEN RECOGNIZED. STATISTICAL ANALYSIS OF THE DATA BY CHI-SQUARE AND STUDENTS T-TEST PROVIDED FURTHER SUPPORT FOR THE SIGNIFICANCE OF THE FINDINGS ALREADY MENTIONED..
Rocket Labs Electron commercial satellite launcher lifted off from New Zealand on its debut test flight Thursday and soared into space on its maiden mission.. The 55-foot-tall (17-meter) launcher did not reach orbit as intended, but Rocket Lab said the Electron successfully flew through the boosters first stage burn, stage separation, second stage ignition and payload fairing separation.. The Electron is designed to loft CubeSats and other small satellites into orbit, and Rocket Lab already has launch contracts with NASA and several commercial customers.. These video clips provided by Rocket Lab show multiple views of the Electron lifting off from its commercial base in New Zealand, plus imagery captured by an on-board camera showing first stage separation, second stage ignition and the release of the rockets payload fairing.. Read our full story on the test launch for details.. Video credits: Rocket Lab. ...
Let V be the total volume of the rocket. Let Vw be the volume of water in the rocket, and Va be the volume of air. Let Vai and Vwi be the inital volumes of air and water, at the time of launch. Let p be the gauge pressure inside the rocket (i.e. the pressure above atmospheric). Let pi be the pressure at the time of launch. Well assume static fluids at all times (this is not really true, but at low escape velocities it is inconsequential). Let m be the mass of the rocket (well assume that the water is the only important mass -- the mass of the air and rocket body will be considered negligible). Let o be the exhaust hole area at the bottom of the rocket, which is considered a plane hole. Let v be the exit velocity of the water. Let h be the height of the water in the rocket ...
Stomp Rocket Jr. Glow is 100% KID powered! Run, jump and STOMP to launch these rockets up to 100 feet in the air! All-foam Jr. Glow Stomp Rockets are great for younger kids. They glow in the dark and are perfect for fun during the day, after dusk and in gloomy weather too. Stomp Rocket Jr. Glow is strong and durable, and great for active, outdoor play. Stomp Rockets have won lots of awards from industry experts, including iParenting Media, Dr. Toy and Creative Child Magazine. Includes 4 all-foam glow-in-the-dark Stomp Rockets and a Stomp Launcher. Refill rockets also available (item #20015). For kids ages 3 and up ...
Atlas Copco Boomer Rocket Boomer M2 C Typical min. drift width 3.2 m Coverage 10 - 45 m² Rocket Boomer 282 Typical min. drift width 3.0 m Coverage 8 - 45 m² Rocket Boomer L1 C Typical min. drift width 3.2 m Rocket Boomer 281 Typical min. drift width 2.7 m Rocket Boomer 104 Typical min. drift width 1.8 m Rocket Boomer S1 L For roof heights ...
Urine immunoelectrophoresis is a lab test that measures immunoglobulins in a urine sample. Immunoglobulins are proteins that function as antibodies. Learn more.
rocket definition: The definition of a rocket is a long circular device that is launched into the air. (noun) An example of a rocket is what helps launch a guided missile into space. An example of a rocket is a firecracker. ...
Whole IgG antibodies are isolated as intact molecules from antisera by immunoaffinity chromatography. They have an Fc portion and two antigen binding Fab portions joined together by disulfide bonds and therefore they are divalent. The average molecular weight is reported to be about 160 kDa. The whole IgG form of antibodies is suitable for the majority of immunodetection procedures and is the most cost effective.Based on immunoelectrophoresis and/or ELISA, the antibody reacts with whole molecule mouse IgG. It also reacts with the light chains of other mouse immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. The antibody has been tested by ELISA and/or solid-phase adsorbed to ensure minimal cross-reaction with bovine, chicken, goat, guinea pig, syrian hamster, horse, human, rabbit and sheep serum proteins, but it may cross-react with immunoglobulins from other species ...
The antibody was raised in Rabbit by immunizing purified Mouse IgG. These antisera were then immunoaffinity purified from the antigen specific column with minimum cross-reaction to human serum proteins. Based on immunoelectrophoresis, the antibody reacts with the heavy chains on Mouse IgG and with the light chains common to most mouse immunoglobulines. No antibody was detected against non-immunoglobulin serum proteins. ...
Shiver of light - (merry gentry 9) (Heftet) av forfatter Laurell K. Hamilton. Pris kr 119. Se flere bøker fra Laurell K. Hamilton.
Rabbit anti-sheep IgG (H/L) is specific for sheep IgG heavy and light chains. Immunoelectrophoresis resulted in a single precipitin arc ag
Still Testing was originally slated to blast off during a launch window in November of last year, but the launch was delayed after a power fault in the ground systems triggered an automatic abort just seconds before liftoff. Once again, were expecting to scrub multiple times as we wait for perfect conditions and make sure everything on the vehicle is performing as it should, said Peter Beck, founder and CEO of Rocket Lab.. The Electron rocket, built with 3D-printed engines and a mostly composite construction, is designed for rapid manufacture and a high launch rate. The relatively small launch vehicle, only 56 feet tall, is catered to the growing demand for a rocket to place smaller satellites in orbit. Ultimately, Rocket Lab wants to ramp up to about 50 Electron launches per year from its private spaceport in New Zealand. Before it can do that, however, Rocket Lab needs to complete its vehicle testing program, which could continue with a launch as early as Friday night. ...
model rocket launch pad for sale - 25 - model rocket launch pad wholesalers & model rocket launch pad manufacturers from China manufacturers.
Rocket Lab successfully launched a rocket from the Hawkes Bays Mahia Peninsula on Sunday, sending its Electron satellite into orbit. The triumph means Rocket Lab will soon be ready to forge ahead in the commercial space industry. To discuss the significance of the launch were joined by Nick Rattenbury, an astrophysicist at the University of Auckland.
Rocket engines. View of the nozzles of rocket engines on the first stage of a Russian Proton-M launcher. The Proton-M has six RD-253 first-stage engines developing a total of 10.5 MN (mega-newtons) of thrust. He rocket has a payload capacity of 22 tonnes to low-earth orbit or 3.5 tonnes to geostationary orbit with the Briz upper stage. Photographed at the Baikonur space centre in Kazkhstan in 2007. - Stock Image C007/3258
How to Make a Rocket. Rockets illustrate Newtons Third Law of Motion: For every action, there is an equal and opposite reaction. The first rocket may have been a steam-propelled wooden pigeon invented by Archytas of Tarentum in the...
When the Rockets needed to get something going offensively late in Sundays game, coach Kevin McHale decided to throw something different at the Trail Blazers. The tactic worked, as the Rockets outscored the Trail Blazers 33-21 in the final quarter - Harden had 17 of those, and Lin had nine. The three guards did provide an offensive spark, but their defense also was key to the victory. In the last several wins for the Rockets, multiple lineups have worked in their favor.
Space Rocket - Star World is a Casual Game, Download Space Rocket - Star World For PC from the direct download link on PC Grim. Space Rocket - Star World is a
Stomp on the launch pad and generate a blast of air that propels a rocket 200 ft into the air. Rocket features a rounded nosecone made of foam for safe launches and landings. Kit includes 4 rockets and a launch pad.
How is Multiple Launch Rocket System Fire Direction System abbreviated? MLRSFDS stands for Multiple Launch Rocket System Fire Direction System. MLRSFDS is defined as Multiple Launch Rocket System Fire Direction System very rarely.
A long time ago, in a galaxy far far away (okay, really it was only over here a few years ago), I wrote an article about plot. The past couple days, Ive been reading Laurell K. Hamiltons latest book (I use that term loosely, based on the fact that it was produced by a publishing company,…
Vassilis Spanoulis got a chance when Kirk Snyders right hand was broken Monday night, then made enough of it to extend his playing time well into the fourth quarter of a 97-90 victory over the Knicks. More than anything, Spanoulis played 13 minutes without the waves of turnovers or defensive gambles that filled his preseason minutes. Van Gundy said Spanoulis is guaranteed increased playing time as long as he can keep his turnovers to a minimum. Theres that fine line between aggression - and he needs to play aggressively on offense - and being careful with the ball, especially as we struggle through this turnover period were going through. [...] his quickness could help offset the trend toward playing two smaller, quick guards, a backcourt style that has given the Rockets trouble. To help negate his turnover tendencies, the Rockets will likely keep Spanoulis at shooting guard, rather than the point guard position he played in the preseason. Shy about shootingWith a shot that is a work in
Nov 28, 2017, 05:01pm EST , Its Not Rocket Science. x ƶ�����L�uKY #�o*Ȝ����E iI�_ݷ[email protected]����C,�a�*Ɩ�������z�o=��>�*~�`)_��cF�B���d��W�\�C���F�Ս���WB��9h�,R���3���f7��7�R�Byx googletag.pubads().setTargeting(cdo_ei, it-s-not-rocket-science); bids: [{ bidder: rubicon, params: { accountId: 17282, siteId: 162036, zoneId: 776156, position: atf }}, }, Not one of us stood up and said, Dammit, stop!. Subscribe to gain access to 3 exclusive newsletter freebies PLUS teaching tips, free resources, and updates about whats going in my Its Not Rocket Science classroom and store each month. Directions: Answer each question, you do not … 3 - Draw an arrow (or more if needed) to show the transfer of electrons and move … Make sure to show all work to receive credit. Then answer the Flow here is the process of energy moving through a system. Hi Im Rebecca and Ive been ...
12 of Rocket League's Battle-Cars have been miniaturized into little pull-back racers! Each one comes packaged up inside a Rocket Ball, which when opened will reveal the surprise of which racer you've got. Select cars include DLC code for in-game content!
Lockout, shmockout. Big deal. Season or not, the Rockets want you to know that buying season tickets is still a safe investment. Fans wont lose a penny if any games are missed. The Rockets are offering two plans for season ticket holders. Season tic… … ...
CLEVELAND -- All-Star guard James Harden fired in 37 points in the Houston Rockets' 118-111 victory over the Cleveland Cavaliers on Saturday at Quicken Loans Arena. He hit the Cavaliers with a 17-point barrage in the third quarter, including a rare four-point play. He helped the Rockets post their
Dnepr rocket also dubbed Satan has blast off into earths orbit carrying over 30 satellites from 17 countries, including the US. The rocket itself is of Russia-Ukraine make.
Specialist training in the operation of advanced rocket launchers. 2% bonus per level to the rate of fire of modules requiring Rocket Specialization ...
Goat anti Mouse IgG antibody recognizes both the heavy and light chains of mouse IgG. Immunoelectrophoresis revealed a single precipitin a
Hello, Im completely new to shooters that require anything besides hitscan aim. Because of this I find aiming with rockets incredibly hard. I cant seem to hit much and most of the hits feel like random luck because I dont really know what Im doing. The only tip Ive gotten so far is to only use rockets if you can see enemys feet. What other sort of stuff should I know about? When do I aim at the floor besides them and when do I go for a direct hit? When should I jump in a rocket vs. rocket fight? How fast should I be shooting? Is there a generally accepted better crosshair to use with rockets, I always seem to aim too far up. And as if thats not hard enough - whats the logic behind dodging? What kind of positioning should I utilize? Do people jump to dodge? How to figure out your opponent while constantly getting hit? I really wish Reflex had bots and aim maps, would make practising a lot more enjoyable. Thanks ...
Many kids don't like dark rooms. Time to add some space-age whimsy (and light) to their wall with this Red Rocket. Remote controlled lights and sounds will help them not be so scared of the dark.
Michael Strano set off hydrogen-fueled rockets as a kid. Today his lab is developing nanosensors, bionic plants, nanoscale ice wires, and a brand-new source of energy.
http://www.serebii.net/anime/NextOn/585.jpgTeam Rocket Disband!? Travelling towards the next town, Team Rocket encounter an old friend whom has set up a Ramen shop in their honour. Seeing how successful he has been since he quit Team Rocket, Jessie & James start considering whether their vocation has taken them as far as its going to. Meanwhile, Ash & Co. near to Team Rocket, enter a valley thats inhabited by an Alternate Coloured Metagross... Visit The Episode Guide Discuss
The amount of data sent to or from mobile gadgets around the world annually will rocket to hit the equivalent of 33 billion full-length DVD films by the year 2016, computer networking titan Cisco forecast on Tuesday.
With Tuesdays addition of Omer Asik and the signing of point guard Jeremy Lin last week, the overhaul of the roster has brought the Rockets the type of young players at key positions they believe they can build around. … ...
Click for info on curbside pickup available at Cycle Gear stores & how were working to ensure a safe shopping experience. The Joe Rocket Womens Cleo Elite Jacket is a versatile mesh jacket with a waterproof liner and an insulated vest. Shop Cycle Gear for our 30-day best price guarantee & hassle free returns.
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The team behind a British attempt to break the land speed record says theyve teamed up with a Norwegian company for the cars rocket engine.
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https://youtu.be/SK-1iXJBI3U Students had fun today with flying rocket experiment. Conducted this activity to introduce reaction of acid with carbonate. Add sprite in film canister, to about 3/4. Add 3 spatulaful of baking soda on the cap. Place the cap over the film canister and shut it tight. Invert the film canister. Step away and watch…
I finally got my revised 3-stage rocket flying again today! Hopefully Ill get into bottle splicing when I get back into it later this year or next year, but…
Deska Rocket Science norských Apoptygma Berzerk už je venku sice hezkých pár týdnů, ale o její recenzi z pera Pavla Zelinky jsme vás nemohli a nechtěli ochudit.
A question came to me via the Ask the Expert service that Reading Rockets provides. With the teachers permission, Im including it here to get your opinion. Question:
A question came to me via the Ask the Expert service that Reading Rockets provides. With the teachers permission, Im including it here to get your opinion. Question:
An extract of mixed dog hair and dandruff from six different dog breeds (alsatian, boxer, collie, poodle, and long-haired and short-haired dachshund) was obtained by mild extraction, centrifugation, dialysis and freeze-drying. Extract of hair and dandruff from the individual dog breeds was obtained …
Semantic Scholar extracted view of [Immunoelectrophoretic examination of blood serum proteins in children with pituitary dwarfism]. by I Owczarczyk
Define rise like a rocket and fall like a stick. rise like a rocket and fall like a stick synonyms, rise like a rocket and fall like a stick pronunciation, rise like a rocket and fall like a stick translation, English dictionary definition of rise like a rocket and fall like a stick. n. 1. a. A rocket engine. b. A vehicle or device propelled by one or more rocket engines, especially such a vehicle designed to travel through space. 2.
Carl-Bertil Laurell (born 28 June 1919 in Uppsala, dead 18 September 2001 in Malmö) was a Swedish medical doctor and researcher. Laurell was Professor of clinical chemistry at Lund University. He named the blood plasma protein Transferrin, and discovered that an inherited lack of Alpha 1-antitrypsin could lead to emphysema. In 1976, he was made a member of the Royal Swedish Academy of Sciences. Carl-Bertil Laurell, biographical entry in Nationalencyclopedin (in Swedish ...
The defined antibody specificity is directed to albumin as tested against cat sera. In immunoelectrophoresis and double radial immunodiffusion (Ouchterlony), using various antiserum concentrations against appropriate concentrations of the immunogen, a single characteristic precipitin line is obtained which shows a reaction of identity with the precipitin lines obtained against cat serum and the purified albumin. As precipitating antiserum to identify or measure cat albumin by a variety of immunodiffusion techniques, including immunoelectrophoresis, single and double radial immunodiffusion (Mancini, Ouchterlony) and electroimmunodiffusion (Laurell). It has not been tested for use in nephelometry, ELISA or immunochemistry, but this does not exclude such use if proper controls are included ...
This list provides a one-stop forum on homemade rockets powered by compressed air and water, and related topics. Water rockets are typically made from discarded plastic soft drink bottles, partially filled with water, pumped with air to high pressure, and released. Single stage rockets can reach 500 feet, with top speeds over 100 miles per hour! Advanced rockets may use other materials, other gases, or no water. There are no rules, except to learn while having fun! The list has a history dating back to 1997, but moved from a private service to Yahoo in 2003 with 170 members and 17,000 messages in the archives. The old home page may be still available at http://cjh.polyplex.org/rockets/list/ Postings by new members are moderated (must be approved), until the new member is promoted to full posting status. This helps keep the group free of spam - spammers are quickly banned. Sorry for any inconvenience this might cause.
Bungee Rocket | Promotional Bungee Rocket imprinted with your logo.: Have fun with this promotional item which is like a fun sling shot. Foam rocket. Pull back, take aim and let them fly! Improved for better performance, better looks and most important of all, better durability. Let out your frustrations by scoring a touchdown with this mini football rocket! Made of lightweight stress foam, this fun little toy is great for the beach, park or football field. Even the most serious business people need a toy to play with! Decorate this item in a variety of colors with a printed name or logo and theyll remember your business forever. Hand out this stress reliever at your next event and send your brand visibility sky-high!
The Kelowna Rockets defeated the Vancouver Giants 5-4 in overtime on Friday night at Prospera Place in Kelowna.. Rockets Captain Cal Foote set up Carsen Twarynski for the winner in overtime as Kelowna came from behind to earn the win.. James Porter stopped 24 of the 28 shots he faced in the winning effort. His record improves to 6-2-2-0 on the season while overall the Rockets improved to 9-6-2-1.. The Rockets opened the scoring as Gordie Ballhorn (2) ripped home a power play marker at 13:53 of the first. Ty Ronning(16) and Tyler Benson(3) responded for the Giants and Vancouver took a 2-1 lead into the middle frame.. Kelowna trailed by one until late in the period when Leif Mattson (6) evened things at 2-2. The Rockets and Giants remained tied after 40 minutes.. The Giants regained a one goal lead but it was short lived as Kole Lind (10) scored a shorthanded marker to knot things up once again. Conner Bruggen-Cate (2) pulled Kelowna ahead on the power play at 11:46, but the Giants kept the ...
i. Reaction of identity: Antigens in two wells are identical (Agl and Ag2). So the reaction with the antibody results in a precisely similar precipitin lines. The precipitin lines dont cross, but form a continuous line. Fusion of the two precipitin lines indicates that the antibody is reacting with epitopes commonly present on the two antigens. ii. Reaction of non-identity: Two non-identical antigens (Agl and Ag3) are in the antigen wells while the antibody well has antibodies to both the antigens. The two antigen-antibody precipitin lines formed differ from each other. Hence the two precipitin lines completely cross (intersect) each other. iii. Reaction of partial identity: The antigenic determinants in the antigens of two wells are partially shared (Agl and Agla). Hence the antibody reacts with both the antigens and forms lines that do not form a complete cross. The precipitin line crosses in only one direction. The extended precipitin line is referred to as a spur. The spur indicates that ...
The aeolipile described in the first century BC (often known as Heros engine) essentially consists of a steam rocket on a bearing. It was created almost two millennia before the Industrial Revolution but the principles behind it were not well understood, and its full potential was not realised for a millennium. The availability of black powder to propel projectiles was a precursor to the development of the first solid rocket. Ninth Century Chinese Taoist alchemists discovered black powder in a search for the elixir of life; this accidental discovery led to fire arrows which were the first rocket engines to leave the ground. It is stated that the reactive forces of incendiaries were probably not applied to the propulsion of projectiles prior to the 13th century. A turning point in rocket technology emerged with a short manuscript entitled Liber Ignium ad Comburendos Hostes (abbreviated as The Book of Fires). The manuscript is composed of recipes for creating incendiary weapons from the ...
The X 4 is an antiaircraft rocket designed by the Germans to be launched from planes. It was manufactured and reported to have been successfully tested, but never reached the point of combat operation. It is a wire-controlled, rocket-propelled, fin-stabilized missile fitted with a proximity fuzed warhead. The propulsion system is a bi-fuel rocket. Stabilization is achieved by means of four large fins fitted to the body of the rocket, and four smaller fins fitted to the tail. The smaller fins bear solenoid-operated control surfaces through which two-dimensional directional control is achieved. These are operated from the parent aircraft by means of a control unit and two insulated wires leading to the rocket. These wires are about 3 3/4 miles long.. Precise information about the warhead and fuzing system has not so far been recovered. The warhead consists of an uncased moulded grain of dinitroglycol-based explosive which depends on high blast effect. The fuze is a combination of acoustic ...
Launch of Russian Proton Rocket with British Inmarsat 5-F2 Sat, Proton M rocket explosion July 2 2013 slow motion full HD, Proton M rocket explosion, NEW VIDEO 1, from Astrium team, July 2, 2013, Rocket Profile - Proton
The rocket motor 1/2A3-2T, price for 4 pieces - Model rocket motor series and is designed to drive a model rocket or space shuttle launched from the ramp.
New Triumph Rocket 3 R Black And Rocket 3 GT Triple Black Limited Editions. The ultimate muscle roadster now meaner and moodier. With just 1,000 of each
Our best selling needle - The addi® Rocket circulars. From the pointy tips to the connection from needle to the cable, these needles will become your go to choice for all your knitting. Organize everything in a beautiful linen needle case from Della Q.. Meet the addi® Rocket, a line of circular needles that combines the best of addi®. Styled after our popular addi Lace, these extra-pointy tips feature the same great plating that gives the classic addi Turbo® its incomparable zip. - Addi. 24″ / 60cm addi® Rocket Circular Needle ...
Our best selling needle - The addi® Rocket circulars. From the pointy tips to the connection from needle to the cable, these needles will become your go to choice for all your knitting.. Meet the addi® Rocket, a line of circular needles that combines the best of addi®. Styled after our popular addi Lace, these extra-pointy tips feature the same great plating that gives the classic addi Turbo® its incomparable zip. - Addi. 16″ / 40cm addi® Rocket Circular Needle ...
The Big Betty is the largest and tallest level 1 model rocket kit available! With a maximum altitude of 800 feet this rocket is simply impressive in flight
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Rocket Fuel is an indica-dominant cannabis strain that emits a scent that’s not unlike its name. Both scent and taste are strongly diesel-like with undertones of sweet spices. Cured nuggets of Rocket Fuel are small, round, and dense, and are colored deep green with long burnt orange pistils....
Tonight (Jan. 19), the private spaceflight company Rocket Lab will once again attempt a second test launch of its small-scale Electron rocket.
She Swings Blue Vol 1 Joint is Jumpin by The Rhythm Rockets @ARTISTdirect.com - Listen to Free Music from She Swings Blue Vol 1 Joint is Jumpin by The Rhythm Rockets. ARTISTdirect.com is where music and people connect.
Risk management methodologies have been widely used by many enterprises for decades. However, with increasing regulatory pressures (such as Sarbanes-Oxley and Basel III) and increasing governance obligations, many corporate leaders are looking to manage risk more effectively. Rocket CorVu solutions can help.. For example, Rocket CorRisk is a purpose-built enterprise risk management application that you can deploy rapidly to mitigate risks proactively. You can monitor risk management activities throughout the organization and automate essential workflows. Stakeholders can be alerted to potential threats, and risk management obligations can be fulfilled. Many companies are actively pursuing risk management approaches that extend beyond mere compliance, but that enable them to improve business performance proactively. CorRisk gives you a risk management framework for monitoring and managing the threats and obstacles to a successful business strategy. Other CorVu solutions help optimize performance ...
Rockets coach Kevin McHale, whose 23-year-old daughter Alexandra died on Nov. 25 from complications related to Lupus, returned from personal leave and coached the team in Saturdays loss to the Mavericks … Tonights game is the second between the teams in four days. The Spurs defeated the Rockets at the AT&T Center on Friday, 114-92 … Teams split last seasons series 2-2, each winning both games on respective home courts.. - Mike Monroe. ...
By Steven Apfelbaum Dames rocket has escaped from gardens and is rapidly invading the surrounding landscapes. Left unchecked, this beautiful, yet lethal plant will wreak havoc on the natural environment, threatening the survival of native plants and degrading habitat and water quality. Dames rocket...
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Florida took a small step Saturday toward becoming the world's ''space transportation hub'' when it launched the nation's first state-sponsored rocket. A 10-foot-long rocket carrying weather-sensing equipment for Florida State University streaked off a U.S. Air Force launch pad at 10 a.m. as about 50 people watched from this remote Panhandle beach 110 miles southwest of Tallahassee.
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Background: The SMAW system (launcher, ammunition and logistics support) was fielded in 1984 as a Marine Corps unique system. At that time, the SMAW included the MK153 Mod 0 launcher, the MK3 Mod 0 HEDP encased rocket, the MK4 Mod 0 practice rocket and the MK217 Mod 0 9mm spotting cartridge. The MK6 Mod 0 encased HEAA rocket is being added to the inventory. The MOD 0 has demonstrated several shortcomings. A series of modifications is currently planned to address the deficiencies. They include a resleeving process for bubbled launch tubes, rewriting/drafting operator and technical manuals, a kit that will reduce environmental intrusion into the trigger mechanism, and an optical sight modification to allow the new HEAA rocket to be used effectively against moving armor targets. Recently fielded were new boresight bracket kits that, when installed, will solve the loss of boresight problem between launch tube and spotting rifle. During Desert Storm, 150 launchers and 5,000 rockets were provided to ...
General Reporting Guidelines Use the POEMS response criteria when determining the disease status for POEMS. Immunofixation (IFE) and immunoelectrophoresis (IEP)...
Quality Export Container Transportation-Liquid Sodium Methoxide of Rocket Chemical for sale - buy cheap Sodium Methoxide Solution from sodiummethoxidesolution manufacturer.
Hello=all, I am very happy that Rama ruleset is confirmed for 1.2. This will mean big things for duel, however it brings up an issue for the atdm gamemode. In atdm you tend to have rounds that last a while, and you need more ammo than duel, especially when youre playing a bigger game like 4v4 for example. Ive found through playing that the ammo limits with the current ruleset are pretty good with atdm, however the new rama ruleset ammo caps wont give players enough options, especially with only 16 rockets. To fix this, I think voting on the arena mutator should change the max ammo as well. So, what should these new ammo values be? Here Ive gathered the values of ammo caps for different versions of reflex and cpma: Current ruleset ammo caps: grenades - 15 rockets - 25 ion - 150 bolt - 10 plasma - 120 Rama ruleset ammo caps: grenades - 10 rockets - 16 ion - 150 bolt - 10 plasma - 100 CPMA CA ammo caps: grenades - 15 rockets - 50 lg - 150 rail - 15 plasma - 50 OLD Reflex ammo caps: grenades - ...
There are 669 calories in 1 serving (8 oz) of Johnny Rockets Half Rings & Half Fries, without sauce. Youd need to walk 175 minutes to burn 669 calories. Visit CalorieKing to see calorie count and nutrient data for all portion sizes.
Along with the protection against weather, this mixture of metals provide an amazing heat insulator, reducing the temperature of the rocket on re-entry of the atmosphere by about 200 degrees Fahrenheit ...
Red Rocket Ale is a Red Ale - American Amber / Red style beer brewed by Bear Republic Brewing Co. in Cloverdale, CA. Score: 91 with 2,636 ratings and reviews. Last update: 10-25-2020.
The Expanse is a TV series on the SyFy channel, based on James Coreys Expanse novel series.. This is the most scientifically accurate TV show Ive ever seen. Bar none.. It also is incredibly close to the novels, which is rare.. First off, they actually have their spacecraft laid out like a skyscraper, the way God and Heinlein intended. Not stupidly like an airplane, like Buster Crabbes silly rocket ship from Just Imagine. Skyscraper spaceships are even more rare than media science fiction displaying no sound in airless space. The Expanse gets a big gold star in RocketCats list for getting spacecraft orientation right. The ships are gorgeous as well.. Secondly, they correctly make the point that in space the most valuable commodity is water. And the political situation hinges on it. True this was from the novels, but the TV show made a point of stating this in cold text printed on the TV screen during the introduction.. There is a tense situation in free fall where the crew is trying to get to ...
The piece of a Chinese rocket thats falling uncontrollably to Earth will mostly burn up on reentry, the Chinese government said Friday.
Re-attach the safety clip to the exhaust of the rocket OR use a grounded (to a grounded rod!) paperclip. Put the weapon in the safe or please dont shoot! status or cut the wires from from the magneto to the trigger. The CAREFULLY grasp the rear of the rocket and CAREFULLY remove it. GENTLY lay it on the ground and get the hell away from it! That thing contains a shaped charge of about a half kilogram of C4 or TNT. The easiest and safest way to unload it (for you) is to pull the trigger and dont have any body parts behind the backblast of the rocket. Your neighbors might not speak to you ever again, especially if you hit their chickenhouse. Just remember to keep yourself clear of the backblast from rockets, recoilless rifles, claymore mines, fougasse, and so on. There is also no truth to the rumor that C-4 suppositories will clean ya out real good. ...
Re-attach the safety clip to the exhaust of the rocket OR use a grounded (to a grounded rod!) paperclip. Put the weapon in the safe or please dont shoot! status or cut the wires from from the magneto to the trigger. The CAREFULLY grasp the rear of the rocket and CAREFULLY remove it. GENTLY lay it on the ground and get the hell away from it! That thing contains a shaped charge of about a half kilogram of C4 or TNT. The easiest and safest way to unload it (for you) is to pull the trigger and dont have any body parts behind the backblast of the rocket. Your neighbors might not speak to you ever again, especially if you hit their chickenhouse. Just remember to keep yourself clear of the backblast from rockets, recoilless rifles, claymore mines, fougasse, and so on. There is also no truth to the rumor that C-4 suppositories will clean ya out real good. ...
It is simple math: there are, oh, 12 or 13 better teams than the Rockets? And, what, a couple better coaches than Adelman?. There will be more better jobs available for Rick than better coaches available for the Rockets.. As you may know, Adelman is one if my favorite coaches. He is smart, honest and understands how to get the best from his players. I dont think it was possible for anyone to get more out of the Rockets cast of characters the last two seasons. That doesnt mean the years werent disappointing, only that the disappointment wasnt the coachs fault.. The Rockets are what they are because of the players they have. You dont like those players, dont blame Adelman.. Saddened by the circumstances, Dream Shakes Tom Martin advocates the candidacy of Spurs assistant Mike Budenholzer.. Stan Van Gundy would be an interesting hire, but one that I would support. Im not sold on bringing back Jeff Van Gundy, because as good a defensive coach as he was, his offenses were THAT bad. Mike ...
View Notes - sysprob3 from AERO 16.01 at MIT. Unified Systems Problem 3 WATER BOTTLE ROCKET PERFORMANCE ANALYSIS Learning Objectives: After completing this systems problem you will have: • Applied
Luis Scola, F/C, HOU Now you know why Scola was such a popular pick among experts in the middle rounds. Through two games, Scola is averaging 27.0 points and 16.0 rebounds. Obviously those averages aren t sustainable unless your name is Wilt Chamberlin, but Scola is proving that he will be a force in the pain for the Rockets this season. No Carl Landry and a limited Yao Ming means the uptick in production will continue for the Argentinian big man. Scola does not help out in blocks like other big men, but there is a real chance he could turn into a 20/10 guy with solid percentages this season ...
|h4||span style=color: #81c341;|Features: Purest Flavor, 2500mAh and 3.5ML Tank Capacity|/span||/h4| The VaporFi Rocket 3 provides a combination of innovation and quality found rarely in sub-ohm vape pens. The |a href=javascript: void(0) class=pop-u
Steve was first introduced to this incredible teaching tool in 2001 while speaking at the U.S. Space Camp for Educators. As part of the week-long experience, teachers learned how to use Homer Hickams Rocket Boys with multi-grade levels and in a variety of subject areas to not only inspire students to work hard to see that their dreams come true, but to also introduce and reinforce skills and concepts in some very creative ways. The documents below are offered by the U.S. Space Camp and the Space Academy for Educators in an effort to bring the wonder, discovery, and exploration of space and rocketry into a wide variety of classrooms.. ...
The Houston Rockets once again find themselves in a predicament determining whether to rebuild or retain their core group of players...
A while back my friend asked an easy way to make a bottle rocket .....so this Instructable was bornMake sure to wear the proper protective gear such as some kind of...
A Chinese Long March 2C rocket failed to reach the proper orbit after launching Thursday, likely destroying China's experimental SJ-11-04 satellite, according to state media.
PHOENIX (AP) -- Chris Paul scored 25 points to lead six Houston players in the double figures and the Rockets, still without injured James Harden, rolled past the Phoenix Suns 112-95 on Friday night.
Immunoelectrophoresis Electrophoresis Ling IT.; Cooksley S.; Bates PA.; Hempelmann E.; Wilson RJM. (1986). "Antibodies to the ... http://medical-dictionary.thefreedictionary.com/counter+immunoelectrophoresis Sherris, John C.; Ryan, Kenneth J.; Ray, C. L. ( ...
Immunoelectrophoresis Aizpurua-Olaizola, Oier; Sastre Torano, Javier; Pukin, Aliaksei; Fu, Ou; Boons, Geert Jan; de Jong, ... "rocket immunoelectrophoresis", affinity electrophoresis may be used as an alternative quantification of the protein. Some of ...
Trpinac, P., Bugarski, O .: Immunoelectrophoresis. Archives of Pharmacy 1959; 5: 257. Pavlović, V., Trpinac, P .: Application ...
Ayrault Jarrier M, Levy G, Polonovski J (August 1963). "[Study of Human Serum Alpha-Lipoproteins by Immunoelectrophoresis]". ...
Roy AK, Neuhaus OW (March 1966). "Identification of rat urinary proteins by zone and immunoelectrophoresis". Proceedings of the ...
Sophisticated applications include ELISA, enzyme-linked immunospot (Elispot), immunofluorescence, and immunoelectrophoresis. ... "Identification of Cytosolic Antigens from GW-39 Adenocarcinoma Cells by Crossed Immunoelectrophoresis and Immunofluorescence". ...
Immunoelectrophoresis ‎ *18:20, 7 November 2014 (diff , hist) . . (+1)‎ . . Good agricultural practice ‎ (→‎Food and ...
Solutions of sodium barbital have also been used as pH buffers for biological research, e.g., in immunoelectrophoresis or in ... Monthony JF, Wallace EG, Allen DM (October 1978). "A non-barbital buffer for immunoelectrophoresis and zone electrophoresis in ...
Immunoelectrophoresis fr:Immunofixation MedlinePlus Encyclopedia: 003543 - "Immunofixation - serum" MedlinePlus Encyclopedia: ... Immunofixation as immunoelectrophoresis, takes place in two steps: The first step is identical for both techniques. It consists ...
... immunoelectrophoresis, immunophenotyping, immunochromatographic assay and cyflometry. One of the earliest examples of ...
... is a clinical immunologist, and a pioneer in the field of clinical immunoelectrophoresis. He was a student of Henry ...
... crossed immunoelectrophoresis, and human specific IgE serologic surveys". Annals of Allergy, Asthma & Immunology. 95 (1): 45-52 ... crossed immunoelectrophoresis, and human specific igE serologic surveys. (Abstract)". Annals of Allergy, Asthma & Immunology. ...
"Characterization of extract of dog hair and dandruff from six different dog breeds by quantitative immunoelectrophoresis. ...
This is the text of choice for immunoelectrophoresis. Discontinuous native protein gel electrophoresis Protein purification ... or as immunoelectrophoresis. Traditionally, two classes of blood proteins are considered: serum albumin and globulin. They are ... or gel electrophoresis Immunoelectrophoresis Immunofixation Native gel electrophoresis QPNC-PAGE Paraprotein Fast parallel ... immunoelectrophoresis, counterelectrophoresis, and capillary electrophoresis. Each method has many variations with individual ...
Countercurrent immunoelectrophoresis has been shown to be an effective research diagnostic method, but has been largely ...
Allozymes, DNA-DNA hybridization, immunoelectrophoresis, restriction site analyses of mitochondrial DNA and serology were used ...
Sodium barbital and barbital have also been used as pH buffers for biological research, e.g., in immuno-electrophoresis or in ...
Immunoelectrophoresis and immunofixation studies help identify the type of immunoglobulin, the clonality of the light chain, ...
Gel electrophoresis Immunodiffusion, Immunoelectrophoresis SDD-AGE Northern blot SDS-polyacrylamide gel electrophoresis ...
CS1 maint: discouraged parameter (link) Ouchterlony O (1970), Handbook of Immunodiffusion and Immunoelectrophoresis, Ann Arbor ...
The precipitated cryoglobulins are examined by immunoelectrophoresis and immunofixation to detect and quantify the presence of ...
... immunoelectrophoresis, and magnetic immunoassay. Antibodies raised against human chorionic gonadotropin are used in over the ...
Pull-down assays are a common variation of immunoprecipitation and immunoelectrophoresis and are used identically, although ...
Dielectrophoresis Electroblotting Gel electrophoresis Gel electrophoresis of nucleic acids Immunoelectrophoresis Isoelectric ...
... can refer to: Immunoelectrophoresis, electrophoresis based separation methods for proteins Inclusion-exclusion principle, a ...
... commonly used to analyse proteins Immunoelectrophoresis, used to separate and characterize biomolecules on basis their ...
Food portal Agarose gel electrophoresis Algaculture Alginic acid Asepsis Carrageenan Immunodiffusion Immunoelectrophoresis Lima ...
... immunoelectrophoresis MeSH E05.196.401.568.250 - counterimmunoelectrophoresis MeSH E05.196.401.568.520 - immunoelectrophoresis ... immunoelectrophoresis MeSH E05.478.605.492.350.350.150 - counterimmunoelectrophoresis MeSH E05.478.605.492.350.350.350 - ... immunoelectrophoresis, two-dimensional MeSH E05.478.605.825 - radioimmunoprecipitation assay MeSH E05.478.610.310 - ...
... immunoelectrophoresis, two-dimensional MeSH E01.450.495.735.830 - radioallergosorbent test MeSH E01.450.495.735.840 - ... immunoelectrophoresis MeSH E01.450.495.735.645.350.350.150 - counterimmunoelectrophoresis MeSH E01.450.495.735.645.350.350.350 ...
Immunoelectrophoresis Definition Immunoelectrophoresis, also called gamma globulin [1] electrophoresis, or immunoglobulin ... Immunoelectrophoresis Gale Encyclopedia of Cancer COPYRIGHT 2002 The Gale Group Inc.. Immunoelectrophoresis. Definition. ... Immunoelectrophoresis Gale Encyclopedia of Medicine, 3rd ed. COPYRIGHT 2006 Thomson Gale. Immunoelectrophoresis. Definition. ... Immunoelectrophoresis. Immunoelectrophoresis is a technique that separates proteins on the basis of both their net charge (and ...
Crossed immunoelectrophoresis is also called two-dimensional quantitative immunoelectrophoresis ad modum Clarke and Freeman or ... Rocket immunoelectrophoresis is one-dimensional quantitative immunoelectrophoresis. The method has been used for quantitation ... fused rocket immunoelectrophoresis ad modum Svendsen and Harboe, affinity immunoelectrophoresis ad modum Bøg-Hansen. Agarose as ... crossed immunoelectrophoresis (two-dimensional quantitative immunoelectrophoresis ad modum Clarke and Freeman or ad modum ...
Serum immunoelectrophoresis is a lab test that measures proteins called immunoglobulins in the blood. Immunoglobulins are ... Serum immunoelectrophoresis is a lab test that measures proteins called immunoglobulins in the blood. Immunoglobulins are ...
Urine immunoelectrophoresis is a lab test that measures immunoglobulins in a urine sample. ... Urine immunoelectrophoresis is a lab test that measures immunoglobulins in a urine sample. ...
Urine immunoelectrophoresis is a lab test that measures immunoglobulins in a urine sample. Immunoglobulins are proteins that ... Urine immunoelectrophoresis is a lab test that measures immunoglobulins in a urine sample. ...
... is a two‐stage process. Electrophoresis is conducted in the first stage and immunoprecipitation using ... immunoelectrophoresis. (. IEP. ). The patient (P) shows an arc that is more dense than the control on the other side of the ... Immunoelectrophoresis differs from blotting techniques because with IE the entire procedure is conducted in an agarose gel and ... Immunoelectrophoresis. Stanley S Levinson, University of Louisville, Louisville, Kentucky, USA Published online: September 2009 ...
The immunoelectrophoresis-serum test is used to measure Ig levels. ... The immunoelectrophoresis-serum test (IEP-serum) is a blood test used to measure the types of Ig present in your blood, ... Immunoelectrophoresis-Serum Test. Medically reviewed by Steven Kim, MD on January 6, 2016. - Written by Darla Burke ... What Is an Immunoelectrophoresis-Serum Test?. Immunoglobulins (Igs) are a group of proteins also known as antibodies. ...
What is immunoelectrophoresis? Meaning of immunoelectrophoresis medical term. What does immunoelectrophoresis mean? ... Looking for online definition of immunoelectrophoresis in the Medical Dictionary? immunoelectrophoresis explanation free. ... immunoelectrophoresis. Also found in: Dictionary, Thesaurus, Acronyms, Encyclopedia, Wikipedia. Immunoelectrophoresis. ... immunoelectrophoresis. (ĭm′yə-nō-ĭ-lĕk′trə-fə-rē′sĭs, ĭ-myo͞o′-). n.. The separation and identification of proteins based on ...
Instruct patient on 24 hour urine collection. To begin the urine collection, have the patient void. DISCARD THIS URINE SPECIMEN. This is best done with the first morning specimen. Note the time and record on the requisition. From then on collect all the urine that is voided and place in container. If more than one container is needed, please mark the first as #1 of 2 and retain on the floor until the collection is complete. The final void should be made 24 hours after the first void described earlier. The specimen should be included with the collection. Refrigerate 24 hour urines during collection. Do not use urine preservatives. Mark each container with patient information, including height and weight, and date and time collection started and finished. Deliver specimens promptly to lab ...
... recovery and follow-up care for Immunoelectrophoresis - urine. ... Urine immunoelectrophoresis is a lab test that measures ... Learn about Immunoelectrophoresis - urine, find a doctor, complications, outcomes, ... Immunoelectrophoresis - urine. Immunoglobulin electrophoresis - urine; Gamma globulin electrophoresis - urine; Urine ...
Immuno-Electrophoresis. Agarose gels have a small number of fixed charges, which cause a phenomenon known as electroendosmosis ... This technique, immuno-electrophoresis, is much faster than immuno-diffusion, and has been applied in a variety of geometries, ... Immunodiffusion and immunoelectrophoresis are useful techniques for these purposes.. If antibody and antigen are present in ... Crossed immunoelectrophoresis of antigens and antiserum. In the first dimension, proteins are separated by standard ...
Immunoelectrophoresis of extracts of 200 intracranial tumours against rabbit anti-glioblastoma serum gave positive results (= ... Immunoelectrophoresis Medulloblastoma Posterior fossa tumours This is a preview of subscription content, log in to check access ... Immunoelectrophoresis of extracts of 200 intracranial tumours against rabbit anti-glioblastoma serum gave positive results (= ... Immunoelectrophoresis in the diagnosis of neuroectodermal and mesodermal intracranial tumours, especially those of the ...
Membrane protein analysis by two-dimensional immunoelectrophoresis Message Subject. (Your Name) has forwarded a page to you ... polyacrylamide gels and immunoelectrophoresis. After separation in the first dimension by electrophoresis in SDS, the proteins ...
... were studied by two-dimensional immunoelectrophoresis (2D-IEP) and 32 and 20 different antigenic components were detected, ... Antigenic Analyses of Two Axenized Strains of Entamoeba Histolytica by Two-Dimensional Immunoelectrophoresis * * Shu Mei Chang ... were studied by two-dimensional immunoelectrophoresis (2D-IEP) and 32 and 20 different antigenic components were detected, ...
... were evaluated in the immunoelectrophoresis (IEP) test for the diagnosis of human hydatid disease. A higher sensitivity was ... Evaluation of Whole and Purified Hydatid Fluid Antigens in the Diagnosis of Human Hydatidosis by the Immunoelectrophoresis Test ... Whole sheep hydatid cyst fluid (WHF) and purified hydatid fluid antigens (PHF) were evaluated in the immunoelectrophoresis (IEP ...
Serum immunoelectrophoresis is a lab test that measures proteins called immunoglobulins in the blood. Immunoglobulins are ... Immunoelectrophoresis - blood. IEP - serum; Immunoglobulin electrophoresis - blood; Gamma globulin electrophoresis; Serum ...
If you wish to reuse any or all of this article please use the link below which will take you to the Copyright Clearance Centers RightsLink service. You will be able to get a quick price and instant permission to reuse the content in many different ways.. ...
Immunoelectrophoresis - serum and urine. In: Chernecky CC, Berger BJ, eds. Laboratory Tests and Diagnostic Procedures. 6th ed. ... Urine immunoelectrophoresis is a lab test that measures immunoglobulins in a urine sample. Immunoglobulins are proteins that ... Serum immunoelectrophoresis is a lab test that measures proteins called immunoglobulins in the blood. Immunoglobulins are ... Immunoelectrophoresis - blood. IEP - serum; Immunoglobulin electrophoresis - blood; Gamma globulin electrophoresis; Serum ...
Mod-04 Lec-06 Immunoelectrophoresis and capillary electrophoresis tutorial of Analytical Technologies in Biotechnology course ... Electrophoresis of nucleic acids - Immunoelectrophoresis and capillary electrophoresis - Introduction and basic concepts - ...
Characterization of Extract of Dog Hair and Dandruff From Six Different Dog Breeds by Quantitative Immunoelectrophoresis. ... Characterization of Extract of Dog Hair and Dandruff From Six Different Dog Breeds by Quantitative Immunoelectrophoresis. ... Examination and characterization of the mixed extract by means of crossed immunoelectrophoresis revealed a precipitation ...
Find out information about immunoelectrophoresis. A combination of the techniques of electrophoresis and immunodiffusion used ... to separate the components of a mixture of antigens and make them visible by... Explanation of immunoelectrophoresis ... immunoelectrophoresis. Also found in: Dictionary, Thesaurus, Medical, Acronyms, Wikipedia. Immunoelectrophoresis. A combination ... immunoelectrophoresis. [¦im·yə·nō·i‚lek·trə·fə′rē·səs] (immunology) A serological procedure in which the components of an ...
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Comparison of counter immunoelectrophoresis with immunoblotting for detection of anti-extractable nuclear antigen antibodies in ... Comparison of counter immunoelectrophoresis with immunoblotting for detection of anti-extractable nuclear antigen antibodies in ... Article: Comparison of counter immunoelectrophoresis with immunoblotting for detection of anti-extractable nuclear antigen ... Anti-extractable nuclear antigen (ENA) antibodies were assayed by counter immunoelectrophoresis (CIE) and immunoblotting in ...
... Ask a Doctor about diagnosis, ... i had an immunoelectrophoresis done got back some results that i am afraid are bad news they found band of free kappa light ... Follow-up: Immunoelectrophoresis lab results, found band of free kappa light chain in urine. Advise? 40 minutes later ... Follow-up: Immunoelectrophoresis lab results, found band of free kappa light chain in urine. Advise? 1 hour later ...
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Immunoelectrophoresis- Use of an electrical field to separate proteins in a mixture (such as blood or urine), on the basis of ... Serum immuno-electrophoresis uses a second antibody that reacts with IgM. A spike in the Ig fraction indicates a large amount ... Immunoelectrophoresis. -Use of an electrical field to separate proteins in a mixture (such as blood or urine), on the basis of ... Serum immunoelectrophoresis uses a second antibody that reacts with IgM. A spike in the Ig fraction indicates a large amount of ...
7 Jul Rocket Immunoelectrophoresis is an adaptation of radial immunodiffusion developed by Laurell. It is also known as ... Rocket immunoelectrophoresis is one-dimensional quantitative immunoelectrophoresis.. Rocket immunoelectrophoresis. - PubMed - ... Rocket immunoelectrophoresis.. Immunoelectrophoresis is a general name for a number of biochemical methods for separation and ... Immunoelectrophoresis is a general name for a number of biochemical methods for separation Rocket immunoelectrophoresis is one- ...
Immunoelectrophoresis. BCH To learn the technique of immunoelectrophoresis. -Technique based on the principles of ... Immunoelectrophoresis. BCH To learn the technique of immunoelectrophoresis. -Technique based on the principles of ... The band begins to disappear at the 1: Rocket immunoelectrophoresis is one-dimensional quantitative immunoelectrophoresis. ... Immunoelectrophoresis is a variation of the Ouchterlony double diffusion in gel . a two-step procedure that combines the ...
Immunoelectrophoresis Reagents & Buffers / Agaroses. Acrylamide 3x cryst. , 99.9%, Agarose High EEO, Agarose Low EEO Regular ... Immunoelectrophoresis Reagents & Buffers / Agaroses Electrophoresis & Immunoelectrophoresis Reagents & Buffers / Agaroses. 10X ...
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  • Immunoelectrophoresis, also called gamma globulin electrophoresis, or immunoglobulin electrophoresis, is a method of determining the blood levels of three major immunoglobulins: immunoglobulin M (IgM), immunoglobulin G (IgG), and immunoglobulin A (IgA). (encyclopedia.com)
  • All variants of immunoelectrophoresis require immunoglobulins, also known as antibodies, reacting with the proteins to be separated or characterized. (wikipedia.org)
  • Serum immunoelectrophoresis is a lab test that measures proteins called immunoglobulins in the blood. (medlineplus.gov)
  • Urine immunoelectrophoresis is a lab test that measures immunoglobulins in a urine sample. (medlineplus.gov)
  • Immunoelectrophoresis variants need immunoglobulins/antibodies to react to proteins to be characterized or separated. (laboratoryinfo.com)
  • Serogrouping of Legionella pneumophila by double diffusion and counter immunoelectrophoresis. (bmj.com)
  • Anti-extractable nuclear antigen (ENA) antibodies were assayed by counter immunoelectrophoresis (CIE) and immunoblotting in patients with systemic lupus erythematosus (SLE). (hku.hk)
  • Counter immunoelectrophoresis for rapid testing of toxin producing Corynebacterium diphtheriae. (bvsalud.org)
  • http://medical-dictionary.thefreedictionary.com/counter+immunoelectrophoresis Sherris, John C. (wikipedia.org)
  • Two dimensional crossed immunoelectrophoresis on coverslips. (bvsalud.org)
  • conversely, when the concentration of the antigen in the agar is fixed, the content of the antibody to be tested can be determined (ie reverse rocket immunoelectrophoresis). (locketoys.com)
  • Sera from the patients were examined by means of crossed immunoelectrophoresis for the occurence and number of precipitating antibody specificites against Pseudomonas aeruginosa. (ox.ac.uk)
  • Immunoelectrophoresis in which the antigen or antibody is labeled with a radioisotope. (dictionary.com)
  • Examination and characterization of the mixed extract by means of crossed immunoelectrophoresis revealed a precipitation pattern composed of 25 antigens, some of which were mutually partially identical, and a high content of dog serum proteins was found. (nih.gov)
  • Diagnostic and prognostic significance of Pseudomonas aeruginosa precipitins determined by means of crossed immunoelectrophoresis. (ox.ac.uk)
  • Immunoelectrophoresis is a general name for a number of biochemical methods for separation and characterization of proteins based on electrophoresis and reaction with antibodies. (wikipedia.org)
  • Immunoelectrophoresis is an umbrella term for a number of biochemical procedures to separate and characterize proteins according to electrophoresis and reaction with antibodies. (laboratoryinfo.com)
  • Immunoelectrophoresis is a powerful analytical technique with high resolving power as it combines separation of antigens by electrophoresis with immunodiffusion against an antiserum. (encyclopedia.com)
  • Antigens in extracts of two strains of Entamoeba histolytica (HT-31 and HK-9) were studied by two-dimensional immunoelectrophoresis (2D-IEP) and 32 and 20 different antigenic components were detected, respectively. (ajtmh.org)
  • Whole sheep hydatid cyst fluid (WHF) and purified hydatid fluid antigens (PHF) were evaluated in the immunoelectrophoresis (IEP) test for the diagnosis of human hydatid disease. (ajtmh.org)
  • The main advantage of immunoelectrophoresis is that a number of antigens can be identified in serum. (microbenotes.com)
  • The antigens from P. siamensis recognised by rabbit hyperimmune serum were analysed by immunoelectrophoresis. (who.int)
  • In contrast to SDS-gel electrophoresis, the electrophoresis in agarose allows native conditions, preserving the native structure and activities of the proteins under investigation, therefore immunoelectrophoresis allows characterization of enzyme activities and ligand binding etc. in addition to electrophoretic separation. (wikipedia.org)
  • Immunoelectrophoresis differs from blotting techniques because with IE the entire procedure is conducted in an agarose gel and blotting is not necessary. (els.net)
  • Fused rocket immunoelectrophoresis is a modification of one-dimensional quantitative immunoelectrophorsis used for detailed measurement of proteins in fractions from protein separation experiments. (wikipedia.org)
  • Immunological methods for protein C measurement involve the Laurell rocket immunoelectrophoresis technique, radioimmunoassay technique or ELISA. (thefreedictionary.com)
  • A diagnosis of multiple myeloma was considered, but findings on serum protein electrophoresis, urine protein electrophoresis, and urine immunoelectrophoresis were normal. (thefreedictionary.com)
  • Rocket immunoelectrophoresis also referred to as electroimmunoassay is a simple, quick, and reproducible method for determining the concentration of a specific protein in a protein mixture. (bossanova.club)
  • Identifying a female specific protein with the aid of immunoelectrophoresis. (laboratoryinfo.com)
  • Immunoelectrophoresis created a breakthrough in protein identification and in immunology. (microbenotes.com)
  • Native human blood was replaced by either radiolabelled antithrombin III or radiolabelled prothrombin in the reaction mixture, which was incubated at 37 degrees C. At various time intervals the serum formed from the incubated blood was withdrawn and analysed by crossed immunoelectrophoresis against anti-(S protein) serum in the second dimension. (biomedsearch.com)
  • Serum free light chain analysis showed measuring lambda light chain of 2.59 g/L. Serum protein electrophoresis revealed low IgG, IgA, and IgM levels, with reported immunoelectrophoresis (IEP) showing monoclonal lambda light chain peak with the monoclonal protein. (springer.com)
  • The profit to be gained by testing Danish blood donors for hepatitis B surface antigen (HBsAg) with a third generation technique instead of the currently used immunoelectrophoresis was investigated by additional screening of 48 750 blood units by radioimmunoassay three weeks after donation. (bmj.com)
  • Most of the 23 donors positive on radioimmunoassay and negative on immunoelectrophoresis were healthy carriers of HBsAg (20) or had asymptomatic chronic liver disease (two). (bmj.com)
  • Immunoelectrophoresis of extracts of 200 intracranial tumours against rabbit anti-glioblastoma serum gave positive results (= precipitation) in all cases of tumours of neuroectodermal origin such as glioblastoma, astrocytoma, oligodendroglioma, ependymoma, neurinoma, and spongioblastoma. (springer.com)
  • After immunoelectrophoresis, the sharpest and sharp conical precipitation peaks appear at the clearest term, up to 2 to 5 cm. (locketoys.com)
  • Immunoelectrophoresis refers to precipitation in agar under an electric field. (microbenotes.com)
  • The use of immunoelectrophoresis in food analysis is limited by the availability of specific antibodies. (microbenotes.com)
  • Three phenotypical variants of normal human serum alpha2-macroglobulin revealed by immunoelectrophoresis and specific antibodies obtained in rabbits are presented. (meta.org)
  • Four types of immunoelectrophoresis (IEP) have been used: electroimmunoassay (EIA also called 'rocket' or 'Laurell rocket'), classical IEP, immunofixation electrophoresis (IFE) and immunoprecipitation of proteins after capillary electrophoresis. (els.net)
  • Immunofixation electrophoresis (IFE) is preferred over immunoelectrophoresis because of its greater sensitivity and simpler interpretation characteristics. (els.net)
  • Immunoelectrophoresis is slower, less sensitive, and more difficult to interpret than Immunofixation electrophoresis. (microbenotes.com)
  • In somewhat chronological order: Immunoelectrophoretic analysis (one-dimensional immunoelectrophoresis ad modum Grabar), crossed immunoelectrophoresis (two-dimensional quantitative immunoelectrophoresis ad modum Clarke and Freeman or ad modum Laurell), rocket-immunoelectrophoresis (one-dimensional quantitative immunoelectrophoresis ad modum Laurell), fused rocket immunoelectrophoresis ad modum Svendsen and Harboe, affinity immunoelectrophoresis ad modum Bøg-Hansen. (wikipedia.org)
  • Rocket immunoelectrophoresis is one-dimensional quantitative immunoelectrophoresis. (wikipedia.org)
  • Rocket immunoelectrophoresis method for the content of serum apolipoprotein A1 and B. (thefreedictionary.com)
  • 7 Jul Rocket Immunoelectrophoresis is an adaptation of radial immunodiffusion developed by Laurell. (bossanova.club)
  • Immunoelectrophoresis is a general name for a number of biochemical methods for separation Rocket immunoelectrophoresis is one-dimensional quantitative immunoelectrophoresis. (bossanova.club)
  • Rocket immunoelectrophoresis (RIEP) is a quantitative detection technique that combines unidirectional immunodiffusion and electrophoresis. (locketoys.com)
  • Lp(a) plasma concentrations were determined by rocket immunoelectrophoresis. (nih.gov)
  • Immunodiffusion and immunoelectrophoresis are useful techniques for these purposes. (nationaldiagnostics.com)
  • This is the basis of both immunodiffusion and immunoelectrophoresis. (nationaldiagnostics.com)
  • Chromatin immunoprecipitation Immunodiffusion Ouchterlony double immunodiffusion Radial immunodiffusion Immunoelectrophoresis Counterimmunoelectrophoresis. (bossanova.club)
  • IMSEAR at SEARO: Serodiagnosis of paragonimiasis by enzyme-linked immunosorbent assay and immunoelectrophoresis. (who.int)
  • Crossed immunoelectrophoresis has been used for studies of proteins in biological fluids, particularly human serum, and biological extracts. (wikipedia.org)
  • Affinity immunoelectrophoresis is based on changes in the electrophoretic pattern of proteins through specific interaction or complex formation with other macromolecules or ligands. (wikipedia.org)
  • Affinity immunoelectrophoresis has been used for estimation of binding constants, as for instance with lectins or for characterization of proteins with specific features like glycan content or ligand binding. (wikipedia.org)
  • Water-soluble membrane proteins may be analyzed by a new, rapid technique that combines electrophoresis on high-resolution sodium dodecyl sulfate (SDS) polyacrylamide gels and immunoelectrophoresis. (sciencemag.org)
  • In addition proteins are separated by gel electrophoresis on the basis of their apparent molecular weight, which is not accomplished by immunoelectrophoresis, but nevertheless immunoelectrophoretic methods are still useful rpcket non-reducing conditions are needed. (bossanova.club)
  • Immunoelectrophoresis is a general name for a number of biochemical methods for separation and characterization of proteins based on electrophoresis and reaction with immunoelectrophoresos. (bossanova.club)
  • Immunoelectrophoresis is a general name for a number of biochemical methods for separation and characterization of proteins based on electrophoresis and. (wonderfulaustralia.info)
  • Immunoelectrophoresis is an older method for qualitative analysis of M-proteins in serum and urine. (microbenotes.com)
  • The electrophoresis may be performed with a small volume of sample in a number of alternative ways with or without a supporting medium: SDS polyacrylamide gel electrophoresis (in short: gel electrophoresis, PAGE, or SDS-electrophoresis), free-flow electrophoresis, electrofocusing, isotachophoresis, affinity electrophoresis, immunoelectrophoresis, counterelectrophoresis, and capillary electrophoresis. (wikipedia.org)
  • Immunoelectrophoresis aids in the diagnosis and evaluation of the therapeutic response in many disease states affecting the immune system. (encyclopedia.com)
  • Diagnosis of ovine hydatidosis by Immunoelectrophoresis. (booktopia.com.au)
  • Some variants of affinity immunoelectrophoresis are similar to affinity chromatography by use of immobilized ligands. (wikipedia.org)
  • To find out possible differences with respect to the effect of the trypsin and chymotrypsin on the three variants, they were treated with the enzymes before immunoelectrophoresis. (meta.org)
  • Crossed immunoelectrophoresis is also called two-dimensional quantitative immunoelectrophoresis ad modum Clarke and Freeman or ad modum Laurell. (wikipedia.org)
  • The process of Immunoelectrophoresis is begun by separating the antigen mixture into component parts through the process of electrophoresis and double immuno-diffusion. (laboratoryinfo.com)
  • It can also be used in Ouchterlony double diffusion and immunoelectrophoresis. (mpbio.com)
  • The sensitivity and resolving power of crossed immunoelectrophoresis is than that of the classical immunoelectrophoretic analysis and there are multiple variations of the technique useful for various purposes. (wikipedia.org)
  • BCH To learn the technique of immunoelectrophoresis. (wonderfulaustralia.info)
  • Testing of Danish blood donors using a third generation technique identified a substantial number of donors positive for HBsAg overlooked by immunoelectrophoresis. (bmj.com)
  • It was Grabar and Williams who first coined the term immunoelectrophoresis in 1953. (laboratoryinfo.com)
  • Immunoelectrophoresis is also used frequently to diagnose multiple myeloma , a disease affecting the bone marrow . (encyclopedia.com)
  • The immunoelectrophoretic analysis ad modum Grabar is the classical method of immunoelectrophoresis. (wikipedia.org)
  • The two isoforms were isolated from human plasma by heparin-Sepharose chromatography, and the purity was investigated by isoelectric focusing and crossed immunoelectrophoresis. (ahajournals.org)
  • It should be noted that, because immunoelectrophoresis is not quantitative, it is being replaced by a procedure called immunofixation, which is more sensitive and easier to interpret. (encyclopedia.com)
  • Characterization of Extract of Dog Hair and Dandruff From Six Different Dog Breeds by Quantitative Immunoelectrophoresis. (nih.gov)
  • Immunoelectrophoresis is performed by placing serum on a slide containing a gel designed specifically for the test. (encyclopedia.com)
  • What Is an Immunoelectrophoresis-Serum Test? (healthline.com)
  • The immunoelectrophoresis-serum test (IEP-serum) is a blood test used to measure the types of Ig present in your blood, especially IgM, IgG, and IgA. (healthline.com)
  • Counter current line absorption immunoelectrophoresis is an alternative diagnostic screening test to counter current electrophoresis in Aleutian disease (AD) eradication programs. (thefreedictionary.com)
  • The product is tested for purity and specificity at final concentration by immunoelectrophoresis. (mpbio.com)
  • An immunoelectrophoresis procedure is performed using the machine as illustrated in the image above. (laboratoryinfo.com)
  • Immunoelectrophoresis lab results, found band of free kappa light chain in urine. (healthcaremagic.com)
  • Follow-up: Immunoelectrophoresis lab results, found band of free kappa light chain in urine. (healthcaremagic.com)
  • Immunofixation is similar to urine immunoelectrophoresis , but it may give more rapid results. (stlukes-stl.com)
  • This rule applies only to the specimen and would appear not to allow ordering of an Immunoelectrophoresis on an additional blood specimen. (thefreedictionary.com)