Immunoelectrophoresis. Medical search

A technique that combines protein electrophoresis and double immunodiffusion. In this procedure proteins are first separated by gel electrophoresis (usually agarose), then made visible by immunodiffusion of specific antibodies. A distinct elliptical precipitin arc results for each protein detectable by the antisera.

Immunoelectrophoresis in which a second electrophoretic transport is performed on the initially separated antigen fragments into an antibody-containing medium in a direction perpendicular to the first electrophoresis.

Immunoelectrophoresis in which immunoprecipitation occurs when antigen at the cathode is caused to migrate in an electric field through a suitable medium of diffusion against a stream of antibody migrating from the anode as a result of endosmotic flow.

Technique involving the diffusion of antigen or antibody through a semisolid medium, usually agar or agarose gel, with the result being a precipitin reaction.

Serum that contains antibodies. It is obtained from an animal that has been immunized either by ANTIGEN injection or infection with microorganisms containing the antigen.

Electrophoresis applied to BLOOD PROTEINS.

Substances elaborated by bacteria that have antigenic activity.

Antibodies which elicit IMMUNOPRECIPITATION when combined with antigen.

A sterile solution containing the by-products of growth products of COCCIDIOIDES IMMITIS, injected intracutaneously as a test for COCCIDIOIDOMYCOSIS.

Serological reactions in which an antiserum against one antigen reacts with a non-identical but closely related antigen.

Substances that are recognized by the immune system and induce an immune reaction.

The sum of the weight of all the atoms in a molecule.

Electrophoresis in which a polyacrylamide gel is used as the diffusion medium.

Proteins that are present in blood serum, including SERUM ALBUMIN; BLOOD COAGULATION FACTORS; and many other types of proteins.

Pregnane derivatives containing two double bonds anywhere within the ring structures.

The species Oryctolagus cuniculus, in the family Leporidae, order LAGOMORPHA. Rabbits are born in burrows, furless, and with eyes and ears closed. In contrast with HARES, rabbits have 22 chromosome pairs.

Field of chemistry that pertains to immunological phenomena and the study of chemical reactions related to antigen stimulation of tissues. It includes physicochemical interactions between antigens and antibodies.

Serum globulins with high molecular weight. (Dorland, 28th ed)

Electrophoresis in which agar or agarose gel is used as the diffusion medium.

The formation of a solid in a solution as a result of a chemical reaction or the aggregation of soluble substances into complexes large enough to fall out of solution.

Chromatography on non-ionic gels without regard to the mechanism of solute discrimination.

Electrophoresis in which a pH gradient is established in a gel medium and proteins migrate until they reach the site (or focus) at which the pH is equal to their isoelectric point.

Substances of fungal origin that have antigenic activity.

A mitosporic fungal genus which causes COCCIDIOIDOMYCOSIS.

Electrophoresis in which cellulose acetate is the diffusion medium.

Polysaccharides found in bacteria and in capsules thereof.

The largest class of organic compounds, including STARCH; GLYCOGEN; CELLULOSE; POLYSACCHARIDES; and simple MONOSACCHARIDES. Carbohydrates are composed of carbon, hydrogen, and oxygen in a ratio of Cn(H2O)n.

Techniques for removal by adsorption and subsequent elution of a specific antibody or antigen using an immunosorbent containing the homologous antigen or antibody.

The complex formed by the binding of antigen and antibody molecules. The deposition of large antigen-antibody complexes leading to tissue damage causes IMMUNE COMPLEX DISEASES.

The processes triggered by interactions of ANTIBODIES with their ANTIGENS.

A group of related diseases characterized by an unbalanced or disproportionate proliferation of immunoglobulin-producing cells, usually from a single clone. These cells frequently secrete a structurally homogeneous immunoglobulin (M-component) and/or an abnormal immunoglobulin.

Serum globulins that migrate to the gamma region (most positively charged) upon ELECTROPHORESIS. At one time, gamma-globulins came to be used as a synonym for immunoglobulins since most immunoglobulins are gamma globulins and conversely most gamma globulins are immunoglobulins. But since some immunoglobulins exhibit an alpha or beta electrophoretic mobility, that usage is in decline.

An electrochemical process in which macromolecules or colloidal particles with a net electric charge migrate in a solution under the influence of an electric current.

A strong acid used as a protein precipitant in clinical chemistry and also as a caustic for removing warts.

Sensitive tests to measure certain antigens, antibodies, or viruses, using their ability to agglutinate certain erythrocytes. (From Stedman, 26th ed)

Tests that are dependent on the clumping of cells, microorganisms, or particles when mixed with specific antiserum. (From Stedman, 26th ed)

A synthetic steroid with antigonadotropic and anti-estrogenic activities that acts as an anterior pituitary suppressant by inhibiting the pituitary output of gonadotropins. It possesses some androgenic properties. Danazol has been used in the treatment of endometriosis and some benign breast disorders.

Serum proteins with an electrophoretic mobility that falls between ALPHA-GLOBULINS and GAMMA-GLOBULINS.

Techniques used to demonstrate or measure an immune response, and to identify or measure antigens using antibodies.

Sites on an antigen that interact with specific antibodies.

Centrifugation with a centrifuge that develops centrifugal fields of more than 100,000 times gravity. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)

Abnormal immunoglobulins characteristic of MULTIPLE MYELOMA.

An organism originally isolated from sewage, manure, humus, and soil, but recently found as a parasite in mammals and birds.

Organic compounds that generally contain an amino (-NH2) and a carboxyl (-COOH) group. Twenty alpha-amino acids are the subunits which are polymerized to form proteins.

Electrophoresis in which discontinuities in both the voltage and pH gradients are introduced by using buffers of different composition and pH in the different parts of the gel column. The term 'disc' was originally used as an abbreviation for 'discontinuous' referring to the buffers employed, and does not have anything to do with the shape of the separated zones.

The etiologic agent of murine typhus (see TYPHUS, ENDEMIC FLEA-BORNE).

Absence or reduced levels of PROTHROMBIN in the blood.

Serologic tests in which a positive reaction manifested by visible CHEMICAL PRECIPITATION occurs when a soluble ANTIGEN reacts with its precipitins, i.e., ANTIBODIES that can form a precipitate.

All blood proteins except albumin ( = SERUM ALBUMIN, which is not a globulin) and FIBRINOGEN (which is not in the serum). The serum globulins are subdivided into ALPHA-GLOBULINS; BETA-GLOBULINS; and GAMMA-GLOBULINS on the basis of their electrophoretic mobilities. (From Dorland, 28th ed)

The pH in solutions of proteins and related compounds at which the dipolar ions are at a maximum.

The major immunoglobulin isotype class in normal human serum. There are several isotype subclasses of IgG, for example, IgG1, IgG2A, and IgG2B.

Abnormal immunoglobulins synthesized by atypical cells of the MONONUCLEAR PHAGOCYTE SYSTEM. Paraproteins containing only light chains lead to Bence Jones paraproteinemia, while the presence of only atypical heavy chains leads to heavy chain disease. Most of the paraproteins show themselves as an M-component (monoclonal gammopathy) in electrophoresis. Diclonal and polyclonal paraproteins are much less frequently encountered.

DYSENTERY caused by intestinal amebic infection, chiefly with ENTAMOEBA HISTOLYTICA. This condition may be associated with amebic infection of the LIVER and other distant sites.

Conjugated protein-carbohydrate compounds including mucins, mucoid, and amyloid glycoproteins.

The restriction of a characteristic behavior, anatomical structure or physical system, such as immune response; metabolic response, or gene or gene variant to the members of one species. It refers to that property which differentiates one species from another but it is also used for phylogenetic levels higher or lower than the species.

A series of steps taken in order to conduct research.

An abnormal protein with unusual thermosolubility characteristics that is found in the urine of patients with MULTIPLE MYELOMA.

Serologic tests based on inactivation of complement by the antigen-antibody complex (stage 1). Binding of free complement can be visualized by addition of a second antigen-antibody system such as red cells and appropriate red cell antibody (hemolysin) requiring complement for its completion (stage 2). Failure of the red cells to lyse indicates that a specific antigen-antibody reaction has taken place in stage 1. If red cells lyse, free complement is present indicating no antigen-antibody reaction occurred in stage 1.

A genus of gram-positive, rod-shaped bacteria whose organisms are nonmotile. Filaments that may be present in certain species are either straight or wavy and may have swollen or clubbed heads.

An excess of GAMMA-GLOBULINS in the serum due to chronic infections or PARAPROTEINEMIAS.

Techniques used to separate mixtures of substances based on differences in the relative affinities of the substances for mobile and stationary phases. A mobile phase (fluid or gas) passes through a column containing a stationary phase of porous solid or liquid coated on a solid support. Usage is both analytical for small amounts and preparative for bulk amounts.

Separation technique in which the stationary phase consists of ion exchange resins. The resins contain loosely held small ions that easily exchange places with other small ions of like charge present in solutions washed over the resins.

One of the types of light chains of the immunoglobulins with a molecular weight of approximately 22 kDa.

Laboratory tests for evaluating the individual's clotting mechanism.

A chromatographic technique that utilizes the ability of biological molecules to bind to certain ligands specifically and reversibly. It is used in protein biochemistry. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)

One of the types of light chain subunits of the immunoglobulins with a molecular weight of approximately 22 kDa.

Inflammation of the coverings of the brain and/or spinal cord, which consist of the PIA MATER; ARACHNOID; and DURA MATER. Infections (viral, bacterial, and fungal) are the most common causes of this condition, but subarachnoid hemorrhage (HEMORRHAGES, SUBARACHNOID), chemical irritation (chemical MENINGITIS), granulomatous conditions, neoplastic conditions (CARCINOMATOUS MENINGITIS), and other inflammatory conditions may produce this syndrome. (From Joynt, Clinical Neurology, 1994, Ch24, p6)

A synthetic steroid that has anabolic and androgenic properties. (From Martindale, The Extra Pharmacopoeia, 30th ed, p1194)

Antigens on surfaces of cells, including infectious or foreign cells or viruses. They are usually protein-containing groups on cell membranes or walls and may be isolated.

An absence or reduced level of Antithrombin III leading to an increased risk for thrombosis.

Represents 15-20% of the human serum immunoglobulins, mostly as the 4-chain polymer in humans or dimer in other mammals. Secretory IgA (IMMUNOGLOBULIN A, SECRETORY) is the main immunoglobulin in secretions.

A basic science concerned with the composition, structure, and properties of matter; and the reactions that occur between substances and the associated energy exchange.

The property of antibodies which enables them to react with some ANTIGENIC DETERMINANTS and not with others. Specificity is dependent on chemical composition, physical forces, and molecular structure at the binding site.

The composition, conformation, and properties of atoms and molecules, and their reaction and interaction processes.

A major protein in the BLOOD. It is important in maintaining the colloidal osmotic pressure and transporting large organic molecules.

Purifying or cleansing agents, usually salts of long-chain aliphatic bases or acids, that exert cleansing (oil-dissolving) and antimicrobial effects through a surface action that depends on possessing both hydrophilic and hydrophobic properties.

A glycoprotein that is central in both the classical and the alternative pathway of COMPLEMENT ACTIVATION. C3 can be cleaved into COMPLEMENT C3A and COMPLEMENT C3B, spontaneously at low level or by C3 CONVERTASE at high level. The smaller fragment C3a is an ANAPHYLATOXIN and mediator of local inflammatory process. The larger fragment C3b binds with C3 convertase to form C5 convertase.

Non-nucleated disk-shaped cells formed in the megakaryocyte and found in the blood of all mammals. They are mainly involved in blood coagulation.

Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.

A glycoprotein that is important in the activation of CLASSICAL COMPLEMENT PATHWAY. C4 is cleaved by the activated COMPLEMENT C1S into COMPLEMENT C4A and COMPLEMENT C4B.

Multi-subunit proteins which function in IMMUNITY. They are produced by B LYMPHOCYTES from the IMMUNOGLOBULIN GENES. They are comprised of two heavy (IMMUNOGLOBULIN HEAVY CHAINS) and two light chains (IMMUNOGLOBULIN LIGHT CHAINS) with additional ancillary polypeptide chains depending on their isoforms. The variety of isoforms include monomeric or polymeric forms, and transmembrane forms (B-CELL ANTIGEN RECEPTORS) or secreted forms (ANTIBODIES). They are divided by the amino acid sequence of their heavy chains into five classes (IMMUNOGLOBULIN A; IMMUNOGLOBULIN D; IMMUNOGLOBULIN E; IMMUNOGLOBULIN G; IMMUNOGLOBULIN M) and various subclasses.

A technique using antibodies for identifying or quantifying a substance. Usually the substance being studied serves as antigen both in antibody production and in measurement of antibody by the test substance.

Plasma glycoprotein member of the serpin superfamily which inhibits TRYPSIN; NEUTROPHIL ELASTASE; and other PROTEOLYTIC ENZYMES.

Proteins found in any species of bacterium.

Process of determining and distinguishing species of bacteria or viruses based on antigens they share.

The clumping together of suspended material resulting from the action of AGGLUTININS.

The lipid- and protein-containing, selectively permeable membrane that surrounds the cytoplasm in prokaryotic and eukaryotic cells.

Glycoproteins with a molecular weight of approximately 620,000 to 680,000. Precipitation by electrophoresis is in the alpha region. They include alpha 1-macroglobulins and alpha 2-macroglobulins. These proteins exhibit trypsin-, chymotrypsin-, thrombin-, and plasmin-binding activity and function as hormonal transporters.

Polypeptide chains, consisting of 211 to 217 amino acid residues and having a molecular weight of approximately 22 kDa. There are two major types of light chains, kappa and lambda. Two Ig light chains and two Ig heavy chains (IMMUNOGLOBULIN HEAVY CHAINS) make one immunoglobulin molecule.

Immunoglobulins produced in a response to BACTERIAL ANTIGENS.

Disorders caused by abnormalities in platelet count or function.

A type of ion exchange chromatography using diethylaminoethyl cellulose (DEAE-CELLULOSE) as a positively charged resin. (From McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)

A methylpentose whose L- isomer is found naturally in many plant glycosides and some gram-negative bacterial lipopolysaccharides.

Immunoglobulin molecules having a specific amino acid sequence by virtue of which they interact only with the ANTIGEN (or a very similar shape) that induced their synthesis in cells of the lymphoid series (especially PLASMA CELLS).

A genus of gram-positive, coccoid bacteria whose organisms occur in pairs or chains. No endospores are produced. Many species exist as commensals or parasites on man or animals with some being highly pathogenic. A few species are saprophytes and occur in the natural environment.

A plasma alpha 2 glycoprotein that accounts for the major antithrombin activity of normal plasma and also inhibits several other enzymes. It is a member of the serpin superfamily.

An iron-binding beta1-globulin that is synthesized in the LIVER and secreted into the blood. It plays a central role in the transport of IRON throughout the circulation. A variety of transferrin isoforms exist in humans, including some that are considered markers for specific disease states.

Classic quantitative assay for detection of antigen-antibody reactions using a radioactively labeled substance (radioligand) either directly or indirectly to measure the binding of the unlabeled substance to a specific antibody or other receptor system. Non-immunogenic substances (e.g., haptens) can be measured if coupled to larger carrier proteins (e.g., bovine gamma-globulin or human serum albumin) capable of inducing antibody formation.

Serum proteins that have the most rapid migration during ELECTROPHORESIS. This subgroup of globulins is divided into faster and slower alpha(1)- and alpha(2)-globulins.

Surface glycoproteins on platelets which have a key role in hemostasis and thrombosis such as platelet adhesion and aggregation. Many of these are receptors.

The outermost layer of a cell in most PLANTS; BACTERIA; FUNGI; and ALGAE. The cell wall is usually a rigid structure that lies external to the CELL MEMBRANE, and provides a protective barrier against physical or chemical agents.

The physical or physiological processes by which substances, tissue, cells, etc. take up or take in other substances or energy.

Passive agglutination tests in which antigen is adsorbed onto latex particles which then clump in the presence of antibody specific for the adsorbed antigen. (From Stedman, 26th ed)

A serine endopeptidase that is formed from TRYPSINOGEN in the pancreas. It is converted into its active form by ENTEROPEPTIDASE in the small intestine. It catalyzes hydrolysis of the carboxyl group of either arginine or lysine. EC 3.4.21.4.

A complex sulfated polymer of galactose units, extracted from Gelidium cartilagineum, Gracilaria confervoides, and related red algae. It is used as a gel in the preparation of solid culture media for microorganisms, as a bulk laxative, in making emulsions, and as a supporting medium for immunodiffusion and immunoelectrophoresis.

Endogenous substances, usually proteins, that are involved in the blood coagulation process.

The aggregation of ERYTHROCYTES by AGGLUTININS, including antibodies, lectins, and viral proteins (HEMAGGLUTINATION, VIRAL).

A class of immunoglobulin bearing mu chains (IMMUNOGLOBULIN MU-CHAINS). IgM can fix COMPLEMENT. The name comes from its high molecular weight and originally being called a macroglobulin.

A high-molecular-weight plasma protein, produced by endothelial cells and megakaryocytes, that is part of the factor VIII/von Willebrand factor complex. The von Willebrand factor has receptors for collagen, platelets, and ristocetin activity as well as the immunologically distinct antigenic determinants. It functions in adhesion of platelets to collagen and hemostatic plug formation. The prolonged bleeding time in VON WILLEBRAND DISEASES is due to the deficiency of this factor.

Compounds and molecular complexes that consist of very large numbers of atoms and are generally over 500 kDa in size. In biological systems macromolecular substances usually can be visualized using ELECTRON MICROSCOPY and are distinguished from ORGANELLES by the lack of a membrane structure.

Hydrolases that specifically cleave the peptide bonds found in PROTEINS and PEPTIDES. Examples of sub-subclasses for this group include EXOPEPTIDASES and ENDOPEPTIDASES.

An immunoassay utilizing an antibody labeled with an enzyme marker such as horseradish peroxidase. While either the enzyme or the antibody is bound to an immunosorbent substrate, they both retain their biologic activity; the change in enzyme activity as a result of the enzyme-antibody-antigen reaction is proportional to the concentration of the antigen and can be measured spectrophotometrically or with the naked eye. Many variations of the method have been developed.

Proteins which are found in membranes including cellular and intracellular membranes. They consist of two types, peripheral and integral proteins. They include most membrane-associated enzymes, antigenic proteins, transport proteins, and drug, hormone, and lectin receptors.

Plasma glycoprotein clotted by thrombin, composed of a dimer of three non-identical pairs of polypeptide chains (alpha, beta, gamma) held together by disulfide bonds. Fibrinogen clotting is a sol-gel change involving complex molecular arrangements: whereas fibrinogen is cleaved by thrombin to form polypeptides A and B, the proteolytic action of other enzymes yields different fibrinogen degradation products.

Polymers of ETHYLENE OXIDE and water, and their ethers. They vary in consistency from liquid to solid depending on the molecular weight indicated by a number following the name. They are used as SURFACTANTS, dispersing agents, solvents, ointment and suppository bases, vehicles, and tablet excipients. Some specific groups are NONOXYNOLS, OCTOXYNOLS, and POLOXAMERS.

Group of hemorrhagic disorders in which the VON WILLEBRAND FACTOR is either quantitatively or qualitatively abnormal. They are usually inherited as an autosomal dominant trait though rare kindreds are autosomal recessive. Symptoms vary depending on severity and disease type but may include prolonged bleeding time, deficiency of factor VIII, and impaired platelet adhesion.

The ability of a substance to be dissolved, i.e. to form a solution with another substance. (From McGraw-Hill Dictionary of Scientific and Technical Terms, 6th ed)

A species of gram-negative, facultatively anaerobic, rod-shaped bacteria (GRAM-NEGATIVE FACULTATIVELY ANAEROBIC RODS) commonly found in the lower part of the intestine of warm-blooded animals. It is usually nonpathogenic, but some strains are known to produce DIARRHEA and pyogenic infections. Pathogenic strains (virotypes) are classified by their specific pathogenic mechanisms such as toxins (ENTEROTOXIGENIC ESCHERICHIA COLI), etc.

A malignancy of mature PLASMA CELLS engaging in monoclonal immunoglobulin production. It is characterized by hyperglobulinemia, excess Bence-Jones proteins (free monoclonal IMMUNOGLOBULIN LIGHT CHAINS) in the urine, skeletal destruction, bone pain, and fractures. Other features include ANEMIA; HYPERCALCEMIA; and RENAL INSUFFICIENCY.

Blood-coagulation factor VIII. Antihemophilic factor that is part of the factor VIII/von Willebrand factor complex. Factor VIII is produced in the liver and acts in the intrinsic pathway of blood coagulation. It serves as a cofactor in factor X activation and this action is markedly enhanced by small amounts of thrombin.

A large lobed glandular organ in the abdomen of vertebrates that is responsible for detoxification, metabolism, synthesis and storage of various substances.

Presence of warmth or heat or a temperature notably higher than an accustomed norm.

An anionic surfactant, usually a mixture of sodium alkyl sulfates, mainly the lauryl; lowers surface tension of aqueous solutions; used as fat emulsifier, wetting agent, detergent in cosmetics, pharmaceuticals and toothpastes; also as research tool in protein biochemistry.

Bacterial polysaccharides that are rich in phosphodiester linkages. They are the major components of the cell walls and membranes of many bacteria.

A genus of gram-positive, aerobic bacteria. Most species are free-living in soil and water, but the major habitat for some is the diseased tissue of warm-blooded hosts.

A polysaccharide-producing species of STREPTOCOCCUS isolated from human dental plaque.

Any of numerous agile, hollow-horned RUMINANTS of the genus Capra, in the family Bovidae, closely related to the SHEEP.

Microscopy using an electron beam, instead of light, to visualize the sample, thereby allowing much greater magnification. The interactions of ELECTRONS with specimens are used to provide information about the fine structure of that specimen. In TRANSMISSION ELECTRON MICROSCOPY the reactions of the electrons that are transmitted through the specimen are imaged. In SCANNING ELECTRON MICROSCOPY an electron beam falls at a non-normal angle on the specimen and the image is derived from the reactions occurring above the plane of the specimen.

A class of lipoproteins of small size (4-13 nm) and dense (greater than 1.063 g/ml) particles. HDL lipoproteins, synthesized in the liver without a lipid core, accumulate cholesterol esters from peripheral tissues and transport them to the liver for re-utilization or elimination from the body (the reverse cholesterol transport). Their major protein component is APOLIPOPROTEIN A-I. HDL also shuttle APOLIPOPROTEINS C and APOLIPOPROTEINS E to and from triglyceride-rich lipoproteins during their catabolism. HDL plasma level has been inversely correlated with the risk of cardiovascular diseases.

Serum glycoproteins participating in the host defense mechanism of COMPLEMENT ACTIVATION that creates the COMPLEMENT MEMBRANE ATTACK COMPLEX. Included are glycoproteins in the various pathways of complement activation (CLASSICAL COMPLEMENT PATHWAY; ALTERNATIVE COMPLEMENT PATHWAY; and LECTIN COMPLEMENT PATHWAY).

Homogeneous pyruvate kinase isolated from yeast by two different methods is indistinguishable from pyruvate kinase in cell-free extract. (1/2055)

In this report, we have compared homogeneous yeast (Saccharomyces cerevisiae) pyruvate kinase to enzyme from cell-free extracts in several different ways: 1) isoelectric focusing of cell-free extracts indicates one peak of pyruvate kinase activity whose isoelectric point is the same as that of the pure enzyme; 2) antibody prepared to the pure enzyme produces a single, fused precipitin line against enzyme in the cell-free extract and pure enzyme; 3) immunoelectrophoresis of cell-free extract produces one precipitin arc which has the same mobility as that of the pure enzyme; and 4) immunoprecipitation of the pure enzyme from cell-free extract with subsequent solubilization in 1% sodium dodecyl sulfate and electrophoresis on sodium dodecyl sulfate-polyacrylamide gels produces a single protein band attributable to pyruvate kinase which co-migrates with the purified enzyme. Within the limits of the sensitivity of the methods employed, we conclude that the homogeneous pyruvate kinase prepared from yeast lysed either by Manton-Gaulin homogenization (Aust, A., Yun, S.-L., and Suelter, C. (1975) Methods Enzymol. 42, 176-182) or by toluolysis (Yun, S.-L., Aust, A.E., and Suelter, C.H. (1977) J. Biol. Chem. 251, 124-128) is identical with pyruvate kinase in cell-free extract. (+info)

Enzymatic and immunological characterization of the Mycobacterium fortuitum complex. (2/2055)

The arylsulfatase isozymes of Mycobacterium fortuitum, M. peregrinum, M. chelonei subsp. chelonei, and M. chelonei subsp. abscessus were examined to determine the isozymal and immunological relationship among the members of the M. fortuitum complex. Cell extracts were subjected to electrophoresis on agarose and polyacrylamide gel, and arylsulfatase activity was localized using beta-naphthyl sulfate as substrate. Unique zymograms were produced for M. fortuitum, M. peregrinum, and M. chelonei which were characteristic for each species. The immunological relationship among the sulfatases was assayed by using immunodiffusion and immunoelectrophoresis followed by sulfatase staining for the enzyme. One of the isozymes of M. fortuitum and M. peregrinum cross-reacted, showing immunological identity. Antisera to sulfatases of M. fortuitum and M. peregrinum did not react with sulfatases of M. chelonei. The characterization of sulfatase isozymes in extracts of organisms in the M. fortuitum complex suggests the division of the M. fortuitum complex into two species, M. fortuitum and M. chelonei, with subspecies designations. (+info)

The phenoloxidases of the ascomycete Podospora anserina. Structural differences between laccases of high and low molecular weight. (3/2055)

In order to investigate the extent of the relationship between the three copper-containing glycoproteins, laccases I, II and III (Mr70000, 80000 and 390000 respectively) of Podospora anserina, the following experiments were carried out on laccases II and III: (a) determination of amino acid composition; (b) determination of N-terminal and C-terminal amino acid; (c) determination of sugar composition; (d) dissociation studies on native and denatured laccases and also after removal of copper from the enzymes; (e) digestion of the carbohydrate moieties with the aid of glycosylhydrolases. A comparison between the results of these experiments and data previously obtained with laccase I allows the following conclusions to be drawn. 1. Laccases II and III are not identical. 2. Neither of these low molecular weight laccases are as complete molecules subunits of the oligomeric laccase I. 3. The possibility of partial identity of amino acid sequences of laccases I and III can not be excluded. 4. Laccase II possibly consists of subunits of Mr37000 whereas laccase III does not. 5. Digestion of 50% of the carbohydrate content leads to complete loss of serological specificity (serological reaction and cross reaction). This finding is discussed with regard to the possible role of the carbohydrate moiety as antigenic determinants and thus as the reason for the immunological relationship. As a consequence, at least three independent structural genes for laccases must be assumed. (+info)

Inhibition of human seminal fluid DNA polymerase by an IgG fraction of seminal plasma from vasectomized men. (4/2055)

Immunoglobulin G (IgG) was isolated from ejaculates of intact and vasectomized men by precipitation with ammonium sulphate and DEAE-cellulose ionexchange chromatography. Velocity centrifugation revealed that all of the IgG from intact males was 7S protein while less than 40% of the seminal IgG of vasectomized men cosedimented with the 7S marker; the remaining, immunologically unidentifiable, protein was considerably smaller and heterogeneous in size. Only the 7S IgG from the post-vasectomy ejaculates inhibited the activity of a DNA polymerase from the seminal fluid of an intact male. These results suggest that formation of antibody reactive with the seminal fluid DNA polymerase is one manifestation of a vasectomy-associated autoimmune response in man. (+info)

Complement activity in middle ear effusions. (5/2055)

Evidence for complement utilization in middle ear fluids (MEF) from patients with otitis media with effusion was sought. It was found that cleavage products of C3, C4 and Factor B could be demonstrated immunochemically in MEF, and that native C3 was present in much lower concentrations than other proteins, relative to their serum concentrations. Haemolytic assays for C1-C5 showed that early complement components are inactivated in MEF. Potential mechanisms for complement utilization in MEF are discussed. (+info)

Enterobacterial common antigen: isolation from Shigella sonnei, purification and immunochemical characterization. (6/2055)

In the studies presented the effective procedure of isolation and purification of enterobacterial common antigen from Shigella sonnei has been elaborated. The method is based on sonification of bacterial suspension in the presence of lysozyme and EDTA and subsequent extraction of the pellet with boiling water. The crude extract of common antigen was purified by fractionation with ethanol and chromatography on silica gel and Sephadex LH-20. The comparison of several extraction procedures of enterobacterial common antigen from Shigella sonnei proved that the method described above is most effective. The purified enterobacterial common antigen preparation obtained preserved full biological activity: antigenicity (precipitation and activity in enzyme-linked immunosorbent assay), immunogenicity in rabbits, ability to coat erythrocytes (passive hemagglutination) and inhibitory activity in passive hemagglutination. The pure enterobacterial common antigen was identified to 90% as a polymer of N-acetyl-D-mannosaminuronic acid and N-acetyl-D-glucosamine (2:1, molar ratio), O-acetylated and containing 3.2% fatty acids (C16:0 and C18:1, not oleic). It contains 5.3% nitrogen, less than 4% protein, less than 0.5% phosphorus and less than 1.6% neutral sugar; glycerol and RNA were not found in the preparation. (+info)

Immune response in the garter snake (Thamnophis ordinoides). (7/2055)

Garter snakes (Thamnophis ordinoides) were immunized with hen egg albumin, human gamma-globulin and Keyhole limpet haemocyanin in Freund's adjuvant. Antibody was consistently detected by radioimmunoelectrophoresis and in three different gamma- and beta-globulin precipitin lines called IgM (approximately or equal to 20S), Ig-1 (approximately or equal to 9S) and Ig-2 (approximately or equal to 8-5S). Early antibody (day 31 after immunization) was frequently Ig-M whereas Ig-2 and especially Ig-1 were detectable for the longest duration (992 days). After immunization with antigen in Freund's adjuvant, Ig-1 serum concentration showed the greatest increase, from almost undetectable levels to the most prominent immunoglobulin in immune serum. (+info)

Plant microbody proteins. Purification and glycoprotein nature of glyoxysomal isocitrate lyase from cucumber cotyledons. (8/2055)

1. Isocitrate lyase from cotyledons of cucumber seedlings (Cucumis sativus) has been purified 100-fold. Two methods of preparing the soluble glyoxylate cycle enzyme are described: an elaborated method which used crude extracts of cucumber cotyledons, and another procedure which started with purified glyoxysomes from 4-day-old cotyledons and included a separation of glyoxysomal matrix enzymes by zonal centrifugation. The product behaved as a single species when tested by (a) polyacrylamide gel electrophoresis in the presence of dodecyl sulfate, (b) zonal centrifugation, and (c) double immunodiffusion against rabbit antibody to isocitrate lyase. 2. Isocitrate lyase of cucumber glyoxysomes exhibited a molecular weight of 255,000 and was composed of four apparently identical subunits of Mr 64,000. An isoelectric point of 5.9 was determined. 3. It was shown that isocitrate lyase is a glycoprotein, (a) by Schiff stain on polyacrylamide gels, (b) by periodate oxidation of the enzyme, subsequent reduction with NaB[3H]4 and electrophoretic analysis of the labelled glycoprotein, and (c) by incorporation of [3H]glucosamine in vivo into a protein which could be precipitated with antibodies to isocitrate lyase and revealed a 64,000-Mr band upon electrophoresis. (+info)

Crossed immunoelectrophoresis is also called two-dimensional quantitative immunoelectrophoresis ad modum Clarke and Freeman or ... Rocket immunoelectrophoresis is one-dimensional quantitative immunoelectrophoresis. The method has been used for quantitation ... Identification of nanomaterial interaction with C3 protein complement and 2D immunoelectrophoresis 2D immunoelectrophoresis is ... As a result, newer immunoelectrophoresis techniques have largely supplanted the conventional immunoelectrophoresis. Homburger, ...

https://en.wikipedia.org/wiki/Immunoelectrophoresis

Electrophoresis Immunoelectrophoresis Ling IT.; Cooksley S.; Bates PA.; Hempelmann E.; Wilson RJM. (1986). "Antibodies to the ... "Counter immunoelectrophoresis". Sherris, John C.; Ryan, Kenneth J.; Ray, C. L. (2004). Sherris medical microbiology: an ...

https://en.wikipedia.org/wiki/Counterimmunoelectrophoresis

Immunoelectrophoresis Aizpurua-Olaizola, Oier; Sastre Torano, Javier; Pukin, Aliaksei; Fu, Ou; Boons, Geert Jan; de Jong, ... "rocket immunoelectrophoresis", affinity electrophoresis may be used as an alternative quantification of the protein. Some of ...

https://en.wikipedia.org/wiki/Affinity_electrophoresis

Immunoelectrophoresis Miller, Linda E. (2021). "18, Immunoproliferative Diseases: Role of the Laboratory in Evaluating ... Immunofixation as immunoelectrophoresis, takes place in two steps: The first step is identical for both techniques. It consists ...

https://en.wikipedia.org/wiki/Immunofixation

Simple and inexpensive, immunoelectrophoresis quickly became a widely-used method of analysis in clinical biology with ... His award-winning development of Immunoelectrophoresis made it possible to identify specific bodily proteins, opening new ... In 1953 Grabar developed immunoelectrophoresis, combining electrophoresis and immunochemical analysis to create an "immuno- ... Arquembourg, Pierre C. (1975). Immunoelectrophoresis: Theory, Methods, Identifications, Interpretation. S. Karger. pp. 6, 12. ...

https://en.wikipedia.org/wiki/Pierre_Grabar

Trpinac, P., Bugarski, O .: Immunoelectrophoresis. Archives of Pharmacy 1959; 5: 257. Pavlović, V., Trpinac, P .: Application ...

https://en.wikipedia.org/wiki/Pavle_Trpinac

Ayrault Jarrier M, Levy G, Polonovski J (August 1963). "[Study of Human Serum Alpha-Lipoproteins by Immunoelectrophoresis]". ...

https://en.wikipedia.org/wiki/Apolipoprotein_D

Roy AK, Neuhaus OW (March 1966). "Identification of rat urinary proteins by zone and immunoelectrophoresis". Proceedings of the ...

https://en.wikipedia.org/wiki/Major_urinary_proteins

Sophisticated applications include ELISA, enzyme-linked immunospot (Elispot), immunofluorescence, and immunoelectrophoresis. ... "Identification of Cytosolic Antigens from GW-39 Adenocarcinoma Cells by Crossed Immunoelectrophoresis and Immunofluorescence". ...

https://en.wikipedia.org/wiki/Antigen-antibody_interaction

Solutions of sodium barbital have also been used as pH buffers for biological research, e.g., in immunoelectrophoresis or in ... Monthony JF, Wallace EG, Allen DM (October 1978). "A non-barbital buffer for immunoelectrophoresis and zone electrophoresis in ...

https://en.wikipedia.org/wiki/Barbital

The technology of immunoelectrophoresis, immunoprecipitation in gel and fluorescent microscopy was introduced. In his days a ...

https://en.wikipedia.org/wiki/Armed_Forces_Institute_of_Pathology_(Pakistan)

... immunoelectrophoresis, immunophenotyping, immunochromatographic assay and cyflometry. One of the earliest examples of ...

https://en.wikipedia.org/wiki/Immunochemistry

Goetz, DW (July 2005). "Cross-reactivity among edible nuts: double immunodiffusion, crossed immunoelectrophoresis, and human ... crossed immunoelectrophoresis, and human specific IgE serologic surveys". Annals of Allergy, Asthma & Immunology. 95 (1): 45-52 ...

https://en.wikipedia.org/wiki/Tree_nut_allergy

Gerald Penn is a clinical immunologist, and a pioneer in the field of clinical immunoelectrophoresis. He was a student of Henry ...

https://en.wikipedia.org/wiki/Gerald_Penn_(immunologist)

"Characterization of extract of dog hair and dandruff from six different dog breeds by quantitative immunoelectrophoresis. ...

https://en.wikipedia.org/wiki/Hypoallergenic_dog_breed

... or as immunoelectrophoresis.[citation needed] Traditionally, two classes of blood proteins are considered: serum albumin and ... or gel electrophoresis Immunoelectrophoresis Immunofixation SDD-AGE Native gel electrophoresis QPNC-PAGE Paraprotein Fast ... immunoelectrophoresis, counterelectrophoresis, and capillary electrophoresis. Each method has many variations with individual ...

https://en.wikipedia.org/wiki/Gel_electrophoresis_of_proteins

Ouchterlony O (1970), Handbook of Immunodiffusion and Immunoelectrophoresis, Ann Arbor, MI: Ann Arbor-Humphrey Science ...

https://en.wikipedia.org/wiki/Auchterlonie

Allozymes, DNA-DNA hybridization, immunoelectrophoresis, restriction site analyses of mitochondrial DNA and serology were used ...

https://en.wikipedia.org/wiki/Academy_of_Natural_Sciences_of_Drexel_University

Ouchterlony O (1970), Handbook of Immunodiffusion and Immunoelectrophoresis, Ann Arbor, MI: Ann Arbor-Humphrey Science ...

https://en.wikipedia.org/wiki/Örjan_Ouchterlony

Sodium barbital and barbital have also been used as pH buffers for biological research, e.g., in immuno-electrophoresis or in ...

https://en.wikipedia.org/wiki/Barbiturate

Immunoelectrophoresis and immunofixation studies help identify the type of immunoglobulin, the clonality of the light chain, ...

https://en.wikipedia.org/wiki/Waldenström_macroglobulinemia

Gel electrophoresis Immunodiffusion, Immunoelectrophoresis SDD-AGE Northern blot SDS-polyacrylamide gel electrophoresis ...

https://en.wikipedia.org/wiki/Agarose_gel_electrophoresis

... is also used widely for a number of other applications, for example immunodiffusion and immunoelectrophoresis, as the ...

https://en.wikipedia.org/wiki/Agarose

The precipitated cryoglobulins are examined by immunoelectrophoresis and immunofixation to detect and quantify the presence of ...

https://en.wikipedia.org/wiki/Cryoglobulinemia

... immunoelectrophoresis, and magnetic immunoassay. Antibodies raised against human chorionic gonadotropin are used in over the ...

https://en.wikipedia.org/wiki/Antibody

Pull-down assays are a common variation of immunoprecipitation and immunoelectrophoresis and are used identically, although ...

https://en.wikipedia.org/wiki/Methods_to_investigate_protein–protein_interactions

Dielectrophoresis Electroblotting Gel electrophoresis Gel electrophoresis of nucleic acids Immunoelectrophoresis Isoelectric ...

https://en.wikipedia.org/wiki/Electrophoresis

... may refer to: Immunoelectrophoresis Inclusion-exclusion principle Integrated electric propulsion Isoelectric point ...

https://en.wikipedia.org/wiki/IEP

... two-dimensional immunoelectrophoresis MeSH H01.181.529.307.437.575 - iontophoresis MeSH H01.181.529.307.437.663 - isoelectric ... immunoelectrophoresis MeSH H01.181.529.307.437.568.250 - counterimmunoelectrophoresis MeSH H01.181.529.307.437.568.520 - ...

https://en.wikipedia.org/wiki/List_of_MeSH_codes_(H01)

... commonly used to analyse proteins Immunoelectrophoresis, used to separate and characterize biomolecules on basis their ...

https://en.wikipedia.org/wiki/Electrophoresis_(disambiguation)

Serum immunoelectrophoresis is a lab test that measures proteins called immunoglobulins in the blood. Immunoglobulins are ... Serum immunoelectrophoresis is a lab test that measures proteins called immunoglobulins in the blood. Immunoglobulins are ...

https://medlineplus.gov/ency/article/003541.htm

... View/. Open. emhj_2001_7_6_907 ... Countercurrent immunoelectrophoresis could detect only 62.0% of cases, whereas the pathology and ultrasound results were ... The results of countercurrent immunoelectrophoresis were compared with pathology and ultrasound reports to determine whether ... ultrasound imaging or countercurrent immunoelectrophoresis?. EMHJ - Eastern Mediterranean Health Journal, 7 (‎6)‎, 907-911, ...

https://apps.who.int/iris/handle/10665/119107

Corradetti, A; Verolini, F; Ilardi, A; Bucci, A; World Health Organization (‎World Health OrganizationWorld Health Organization, 1966)‎ ...

https://extranet.who.int/iris/restricted/browse?locale-attribute=en&type=mesh&authority=Immunoelectrophoresis

LOINC Code 49274-4 Immunoelectrophoresis for Serum or Plasma Narrative ... 49274-4Immunoelectrophoresis for Serum or Plasma NarrativeActive. Fully-Specified Name. Component. Interpretation. Property. ... Immunoelectrophoresis. Additional Names. Short Name. Interpretation SerPl IEP-Imp. Display Name. Interpretation IEP Nar [Interp ...

https://loinc.org/49274-4/

Urine immunoelectrophoresis. Light-chain proteins are best detected and identified using immunoelectrophoresis with ... Serum protein electrophoresis and immunoelectrophoresis. These can be used to evaluate and quantitate the abnormal monoclonal ...

https://www.medscape.com/answers/244082-192320/what-is-the-role-of-urinalysis-in-the-workup-of-light-chain-nephropathy

Detection of Bacteroides infection by counter immunoelectrophoresis test.. Authors: Lalitha, M K. Mathai, E. Elias, L. Anandi, ... Detection of Bacteroides infection by counter immunoelectrophoresis test. Indian Journal of Medical Research. 1991 May; 93(): ...

https://imsear.searo.who.int/handle/123456789/23101

keywords = "immunoelectrophoresis, two-dimensional electrophoresis, α-GPD null mutants",. author = "Lee, {Chi Yu} and David ... Mutants which express no apparent α-GPDH activity were analyzed by two-dimensional gels and immunoelectrophoresis in an attempt ... Mutants which express no apparent α-GPDH activity were analyzed by two-dimensional gels and immunoelectrophoresis in an attempt ... Mutants which express no apparent α-GPDH activity were analyzed by two-dimensional gels and immunoelectrophoresis in an attempt ...

https://researchexperts.utmb.edu/en/publications/analyses-of-genetic-variants-of-l-glycerol-3-phosphate-dehydrogen

Immunoelectrophoresis of Cataractous Lenses. AMA Arch Ophthalmol. 1959;61(5):738-744. doi:10.1001/archopht.1959.00940090740010 ...

https://jamanetwork.com/journals/jamaophthalmology/article-abstract/625832

CIEP, counter-immunoelectrophoresis; IAT, serum iodine agglutination test; NA, samples not available; ND, assays not done; +, ...

https://wwwnc.cdc.gov/eid/article/20/12/14-0289-t2

HI, CF, NT, IFA, counter immunoelectrophoresis Footnotes. ,,,Click on the PDF icon to the left to view a copy of this virus ...

https://wwwn.cdc.gov/Arbocat/VirusDetails.aspx?ID=460&SID=4

Guillain-Barré syndrome (GBS) can be described as a collection of clinical syndromes that manifests as an acute inflammatory polyradiculoneuropathy with resultant weakness and diminished reflexes. With poliomyelitis under control in developed countries, GBS is now the most important cause of acute flaccid paralysis.

http://emedicine.medscape.com/article/315632-workup

Immunoelectrophoresis * Immunoglobulin A / isolation & purification * Immunoglobulin G / isolation & purification * ...

https://pubmed.ncbi.nlm.nih.gov/1091417/?dopt=Abstract

The Diagnosis of Human Pulmonary Hydatidosis by the Immunoelectrophoresis Test Luis A. Yarzábal ...

https://www.ajtmh.org/search?f_0=author&q_0=Luis+Yarzabal

Immunoelectrophoresis. Samples of IgM or PSA-IgM preparations (10 μL) were subjected to immunoelectrophoresis on 1% agar gel ...

https://www.hindawi.com/journals/dm/2013/923819/

Categories: Immunoelectrophoresis Image Types: Photo, Illustrations, Video, Color, Black&White, PublicDomain, ...

https://phil.cdc.gov/AdvancedSearchResults.aspx?Search=Immunoelectrophoresis&parentid=11396&catid=13744

2-D Immunoelectrophoresis use Immunoelectrophoresis. Two-Dimensional 2-D Polyacrylamide Gel Electrophoresis use Electrophoresis ...

https://decs.bvs.br/I/DeCS2017_Alfab-0a9.htm

Amyotrophic lateral sclerosis (ALS) is the most common degenerative disease of the motor neuron system. The disorder is named for its underlying pathophysiology, with

https://emedicine.medscape.com/article/1170097-workup

6.1.3 Immunoelectrophoresis. 6.1.3.1 Immunoelectrophoresis Market Estimates and Forecasts, 2014 - 2025 (USD Million). 6.1.4 ... 38 Global immunoelectrophoresis surgery market, 2014 - 2025 (USD Million) Fig. 39 Global antibody identification surgery market ...

https://www.grandviewresearch.com/industry-analysis/mammalian-polyclonal-igg-antibody-market/toc

Immunoelectrophoresis, Immunohistochemistry, Immunocytochemistry, Antibody Identification, Western Blotting), End Users ( ... 5.2.3. Immunoelectrophoresis. 5.2.4. Immunohistochemistry. 5.2.5. Immunocytochemistry. 5.2.6. Antibody Identification. 5.2.7. ... Immunoelectrophoresis. - Immunohistochemistry. - Immunocytochemistry. - Antibody Identification. - Western Blotting. Global ... Immunoelectrophoresis, Immunohistochemistry, Immunocytochemistry, Antibody Identification, Western Blotting), End Users ( ...

https://www.visiongain.com/report/mammalian-polyclonal-igg-antibody-market-report-2020-2030/

Avibacterium paragallinarum; Coturnix japonica; antiserum; bacterial antigens; chickens; farmers; immunoelectrophoresis; ...

https://pubag.nal.usda.gov/?f%5Bpublication_year_rev_facet%5D%5B%5D=7981-2019&f%5Bsource%5D%5B%5D=2019+v.48+no.2&f%5Bsubject_facet%5D%5B%5D=Avibacterium+paragallinarum&f%5Bsubject_facet%5D%5B%5D=chickens&f%5Bsubject_term%5D%5B%5D=bacterial+antigens&f%5Btext_availability_facet%5D%5B%5D=Citation+in+PubAg&per_page=50&sort=date-desc

Antigenic Homogeneity among Legionella pneumophila Serogroups 1 to 6 Evaluated by Crossed Immunoelectrophoresis JETTE M. ... In addition, reproducibility of the antigenic profile of L. pneumophila by crossed immunoelectrophoresis was demonstrated. ... pneumophila serogroup 1 system and the polyvalent crossed immunoelectrophoresis system with L. pneumophila serogroups 1 to 6 ...

https://www.microbiologyresearch.org/content/journal/ijsem/36/3

Immunoelectrophoresis (IEP) 5 * Immunofluorescence (IF) 4 * Immunofluorescence (fixed cells) (IF/ICC) 3 ...

https://www.antikoerper-online.de/antibody/6995381/anti-Complement+4+C4+AA+1450-1723+antibody/

Immunoelectrophoresis performed using serum and urine transthyretin samples revealed no monoclonal spike. The results of ...

https://journals.lww.com/jcma/Fulltext/2022/11000/Prompt_diagnosis_of_a_wild_type_transthyretin.12.aspx

Various electrophoresis systems, including; SDS-PAGE, Western blot and immunoelectrophoresis. *ELISA. *Fluorescence microscopy ...

https://www.worc.ac.uk/courses/human-biology-mphilphd

... counter-immunoelectrophoresis and radio-immune precipitation assay. An enzyme-linked immunosorbent assay (ELISA) that detects ...

https://www.emro.who.int/emhj-volume-16-2010/volume-16-issue-4/article-02.html

The next step is to use immunoelectrophoresis to determine the exact type of proteins in the blood. During this process, ...

https://www.healthline.com/health/m-protein

Additionally, serum samples were screened for anti-pseudomonal antibodies using crossed immunoelectrophoresis 13. To assess ...

https://erj.ersjournals.com/content/26/3/458

Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum. No reaction was observed against ...

https://www.abcam.com/goat-fab2-mouse-iggigmiga-hl-tritc-preadsorbed-ab6004.html

Quantitative immunoelectrophoresis of proteins in human erythrocyte membranes. Analysis of protein bands obtained by sodium ...

https://www.semanticscholar.org/topic/Hymenophyllum-palmatifidum/1375893

RÉSUMÉ L'objectif de cette étude réalisée en Iraq était de définir la sensibilité et la spécificité d'un test ELISA commercial pour la détection de l'antigène de Giardia lamblia dans les selles. (who.int)
Frequencies of autoantibodies to Sm, nRNP, Ro (SSA) and La (SSB) were determined by countercurrent immunoelectrophoresis (CIE) and/or enzyme linked immunosorbent assays (ELISA) in 106 whites and 60 blacks with systemic lupus erythematosus. (nih.gov)
Based on immunoelectrophoresis and/or ELISA, the antibody reacts with whole molecule rat IgG. (jacksonimmuno.com)
Most workers reacted positively with Micropolyspora-faeni antigen, but is was not possible with either ELISA or counter immunoelectrophoresis to separate the ill from the workers who were not ill. (cdc.gov)
Specificity By immunoelectrophoresis and ELISA, this antibody reacts specifically with human IgE. (qedbio.com)
1975). An antigen detection ELISA and counter-immunoelectrophoresis have been used to detect infections of Brugia pahangi in cats (Au et al. (aavp.org)

Serum immunoelectrophoresis is a lab test that measures proteins called immunoglobulins in the blood. (medlineplus.gov)
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum. (abcam.com)
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Mouse IgA alpha and anti-Mouse serum. (novusbio.com)
Based on Immunoelectrophoresis, no reactivity is observed to: non-immunoglobulin rabbit serum proteins, serum proteins from bovine, human, or mouse , IgG from human or mouse. (thermofisher.com)
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti- Rat IgG, anti-Rat IgG F(ab')2 and anti-Rat Serum. (rockland.com)

Lee, CY, Niesel, D & Bewley, GC 1980, ' Analyses of genetic variants of l-glycerol-3-phosphate dehydrogenase in Drosophila melanogaster by two-dimensional gel electrophoresis and immunoelectrophoresis ', Biochemical Genetics , vol. 18, no. 9-10, pp. 1003-1018. (utmb.edu)
Methods for detection of Bence Jone proteins include conventional high-resolution electrophoresis or capillary zone electrophoresis followed by confirmation through immunofixation electrophoresis (preferable) or immunoelectrophoresis. (richardvigilantebooks.com)

The proteins of 46 human bile specimens, collected by several different routes have been studied by crossed immunoelectrophoresis, by rocket immunoelectrophoresis and by radioimmunoassay. (bmj.com)
Rocket Immunoelectrophoresis (RIEP) also known as electro-immuno diffusion is a simple, quick and reproducible method for determining the concentration of antigen (Ag) in an unknown sample. (microbiologynote.com)

Diagnosis of cystic echinococcosis: ultrasound imaging or countercurrent immunoelectrophoresis? (who.int)
The results of countercurrent immunoelectrophoresis were compared with pathology and ultrasound reports to determine whether serological tests could be helpful for diagnosis. (who.int)
Countercurrent immunoelectrophoresis could detect only 62.0% of cases, whereas the pathology and ultrasound results were positive for 96.3% of cases. (who.int)

including immunofluorescent assay, enzyme immunoassay, counter-immunoelectrophoresis and radio-immune precipitation assay. (who.int)

Light-chain proteins are best detected and identified using immunoelectrophoresis with monospecific antikappa and antilambda sera. (medscape.com)
Mutants which express no apparent α-GPDH activity were analyzed by two-dimensional gels and immunoelectrophoresis in an attempt to identify and characterize the inactive proteins. (utmb.edu)
The next step is to use immunoelectrophoresis to determine the exact type of proteins in the blood. (healthline.com)
Quantitative immunoelectrophoresis of proteins in human erythrocyte membranes. (semanticscholar.org)
In the past, specific proteins were analyzed using a variety of specialized methods, such as radial immunodiffusion, immunoelectrophoresis or using dedicated nephelometers. (roche.com)

The most valuable serologic test in the diagnosis of human hydatid disease is immunoelectrophoresis. (medscape.com)

IMSEAR at SEARO: Detection of Bacteroides infection by counter immunoelectrophoresis test. (who.int)

The purified enzyme was free of other known brush border enzymes and appeared homogeneous in immunoelectrophoresis and polyacrylamide gel electrophoresis in the presence of SDS. (nih.gov)
Electrophoresis and immunoelectrophoresis of concentrated urine. (brighamandwomens.org)
Immunoelectrophoresis (IEP) was the first practical method that combined electrophoresis and immunoprecipitation for identifying and characterizing proteins within complex mixtures. (biofron.com)
Comparisons of the migration patterns of various protein fractions in gels by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, line-rocket immunoelectrophoresis, and enzyme-specific staining confirmed the identity of the two strains of R . quintana and their similarity to the vole agent. (microbiologyresearch.org)
The isolated protein was homogenous by the criteria of polyacrylamide gel electrophoresis, gel filtration chromatography, immuno-double diffusion and immunoelectrophoresis. (ias.ac.in)

Anti-Apolipoprotein B, goat polyclonal, recognizes human apolipoprotein B. It is validated for Western blotting, immunoelectrophoresis, and immunoprecipitation. (merckmillipore.com)
Immunoelectrophoresis in which immunoprecipitation occurs when antigen at the cathode is caused to migrate in an electric field through a suitable medium of diffusion against a stream of antibody migrating from the anode as a result of endosmotic flow. (lookformedical.com)

Streptococcus group B typing: comparison of counter-immunoelectrophoresis with the precipitin method. (nih.gov)
The different elapid venoms, with the exception of that of N. naja oxiana , showed a considerable number of identical and similar precipitin components by immunodiffusion and immunoelectrophoresis. (ajtmh.org)
In immunoelectrophoresis and radial immunodiffusion (Ouchterlony), using various antiserum concentrations against fresh normal swine plasma a single precipitin line is obtained which shows a reaction of identity with the precipitin line obtained with purified alpha-1antitrypsin. (lsbio.com)

Diagnosis of cystic echinococcosis: ultrasound imaging or countercurrent immunoelectrophoresis? (who.int)
The results of countercurrent immunoelectrophoresis were compared with pathology and ultrasound reports to determine whether serological tests could be helpful for diagnosis. (who.int)

Urine immunoelectrophoresis revealed kappa light chains. (hindawi.com)
Immunofixation is similar to urine immunoelectrophoresis , but it may give more rapid results. (healthing.ca)

In precipitating techniques as immunoelectrophoresis and single or double radial immunodiffusion to identify the presence of alpha-1antitrypsin in swine plasma or other body fluids or to determine its concentration. (lsbio.com)

Weak serological cross-reactivity was observed by haemagglutination and two-dimensional immunoelectrophoresis. (microbiologyresearch.org)
Minimum cross-reactivity is observed to the light chains or to non-immunoglobulin goat serum proteins based on immunoelectrophoresis. (agrisera.com)

Light-chain proteins are best detected and identified using immunoelectrophoresis with monospecific antikappa and antilambda sera. (medscape.com)
Monospecific as determined by immunoelectrophoresis (IEP) against 2X pooled human serum and neat pooled human plasma. (merckmillipore.com)

Protein immunoelectrophoresis of serum and urine from the proband and from 19 family members was performed to detect monoclonal immunoproteins. (nih.gov)
Within this family, a sibship of seven included three individuals (including the proband) with histologically verified MM and two individuals with a monoclonal gammopathy of unknown significance (MGUS), as determined by immunoelectrophoresis of serum and urine. (nih.gov)
A finding of increased rouleaux formation suggests multiple myeloma, and the workup should be directed toward immunoelectrophoresis of serum and urine. (medscape.com)

Antisera forms multiple arcs against normal human serum by immunoelectrophoresis. (leebio.com)
The affinity purified anti human haptoglobin forms a single preciptin band with human serum haptoglobin and purified human haptoglobin by immunoelectrophoresis and double diffusion. (leebio.com)

Summary: The partial characterization of a mouse hydrosoluble testis specific protein by crossed immunoelectrophoresis, ion‐exchange chromatography and SDS‐Page was made. (uchile.cl)

Serum immunoelectrophoresis showed IgM K-type monoclonal gammopathy. (biomedcentral.com)

This antibody reacts with the heavy chains on goat IgG based on immunoelectrophoresis. (agrisera.com)

In crossed immunoelectrophoresis the polymer reacted with specific antisera to serotypes. (microbiologyresearch.org)

Specificity of hyper immune sera raised against CPS was tested by counter current immunoelectrophoresis. (ijcea.org)

A manual of quantitative immunoelectrophoresis: methods and applications. (microbiologyresearch.org)

The development of a Klebsiella serotyping method by counter-current immunoelectrophoresis (CIE) is described. (nih.gov)

Anatomy5

Organisms10

Diseases9

Chemicals and Drugs61

Analytical, Diagnostic and Therapeutic Techniques and Equipment34

Phenomena and Processes13

Disciplines and Occupations2

Technology, Industry, Agriculture2

Health Care1

Homogeneous pyruvate kinase isolated from yeast by two different methods is indistinguishable from pyruvate kinase in cell-free extract. (1/2055)

Enzymatic and immunological characterization of the Mycobacterium fortuitum complex. (2/2055)

The phenoloxidases of the ascomycete Podospora anserina. Structural differences between laccases of high and low molecular weight. (3/2055)

Inhibition of human seminal fluid DNA polymerase by an IgG fraction of seminal plasma from vasectomized men. (4/2055)

Complement activity in middle ear effusions. (5/2055)

Enterobacterial common antigen: isolation from Shigella sonnei, purification and immunochemical characterization. (6/2055)

Immune response in the garter snake (Thamnophis ordinoides). (7/2055)

Plant microbody proteins. Purification and glycoprotein nature of glyoxysomal isocitrate lyase from cucumber cotyledons. (8/2055)

Immunoelectrophoresis

Immunoelectrophoresis, Two-Dimensional

Counterimmunoelectrophoresis

Immunodiffusion

Immune Sera

Blood Protein Electrophoresis

Antigens, Bacterial

Precipitins

Coccidioidin

Cross Reactions

Antigens

Molecular Weight

Electrophoresis, Polyacrylamide Gel

Blood Proteins

Pregnadienes

Rabbits

Immunochemistry

Macroglobulins

Electrophoresis, Agar Gel

Chemical Precipitation

Chromatography, Gel

Isoelectric Focusing

Antigens, Fungal

Coccidioides

Electrophoresis, Cellulose Acetate

Polysaccharides, Bacterial

Carbohydrates

Immunosorbent Techniques

Antigen-Antibody Complex

Antigen-Antibody Reactions

Paraproteinemias

gamma-Globulins

Electrophoresis

Trichloroacetic Acid

Hemagglutination Tests

Agglutination Tests

Danazol

Beta-Globulins

Immunologic Techniques

Epitopes

Ultracentrifugation

Myeloma Proteins

Acholeplasma laidlawii

Amino Acids

Electrophoresis, Disc

Rickettsia typhi

Hypoprothrombinemias

Sepharose

Precipitin Tests

Serum Globulins

Isoelectric Point

Immunoglobulin G

Paraproteins

Dysentery, Amebic

Glycoproteins

Species Specificity

Methods

Bence Jones Protein

Complement Fixation Tests

Actinomyces

Hypergammaglobulinemia

Chromatography

Chromatography, Ion Exchange

Immunoglobulin kappa-Chains

Blood Coagulation Tests

Chromatography, Affinity

Immunoglobulin lambda-Chains

Meningitis

Stanozolol

Antigens, Surface

Antithrombin III Deficiency

Immunoglobulin A

Chemistry

Antibody Specificity

Chemical Phenomena

Serum Albumin

Detergents

Complement C3

Blood Platelets

Fluorescent Antibody Technique