Immunoelectrophoresis: A technique that combines protein electrophoresis and double immunodiffusion. In this procedure proteins are first separated by gel electrophoresis (usually agarose), then made visible by immunodiffusion of specific antibodies. A distinct elliptical precipitin arc results for each protein detectable by the antisera.Immunoelectrophoresis, Two-Dimensional: Immunoelectrophoresis in which a second electrophoretic transport is performed on the initially separated antigen fragments into an antibody-containing medium in a direction perpendicular to the first electrophoresis.Counterimmunoelectrophoresis: Immunoelectrophoresis in which immunoprecipitation occurs when antigen at the cathode is caused to migrate in an electric field through a suitable medium of diffusion against a stream of antibody migrating from the anode as a result of endosmotic flow.Immunodiffusion: Technique involving the diffusion of antigen or antibody through a semisolid medium, usually agar or agarose gel, with the result being a precipitin reaction.Immune Sera: Serum that contains antibodies. It is obtained from an animal that has been immunized either by ANTIGEN injection or infection with microorganisms containing the antigen.Blood Protein Electrophoresis: Electrophoresis applied to BLOOD PROTEINS.Antigens, Bacterial: Substances elaborated by bacteria that have antigenic activity.Precipitins: Antibodies which elicit IMMUNOPRECIPITATION when combined with antigen.Coccidioidin: A sterile solution containing the by-products of growth products of COCCIDIOIDES IMMITIS, injected intracutaneously as a test for COCCIDIOIDOMYCOSIS.Cross Reactions: Serological reactions in which an antiserum against one antigen reacts with a non-identical but closely related antigen.Antigens: Substances that are recognized by the immune system and induce an immune reaction.Molecular Weight: The sum of the weight of all the atoms in a molecule.Electrophoresis, Polyacrylamide Gel: Electrophoresis in which a polyacrylamide gel is used as the diffusion medium.Blood Proteins: Proteins that are present in blood serum, including SERUM ALBUMIN; BLOOD COAGULATION FACTORS; and many other types of proteins.Pregnadienes: Pregnane derivatives containing two double bonds anywhere within the ring structures.Rabbits: The species Oryctolagus cuniculus, in the family Leporidae, order LAGOMORPHA. Rabbits are born in burrows, furless, and with eyes and ears closed. In contrast with HARES, rabbits have 22 chromosome pairs.Immunochemistry: Field of chemistry that pertains to immunological phenomena and the study of chemical reactions related to antigen stimulation of tissues. It includes physicochemical interactions between antigens and antibodies.Macroglobulins: Serum globulins with high molecular weight. (Dorland, 28th ed)Electrophoresis, Agar Gel: Electrophoresis in which agar or agarose gel is used as the diffusion medium.Chemical Precipitation: The formation of a solid in a solution as a result of a chemical reaction or the aggregation of soluble substances into complexes large enough to fall out of solution.Chromatography, Gel: Chromatography on non-ionic gels without regard to the mechanism of solute discrimination.Isoelectric Focusing: Electrophoresis in which a pH gradient is established in a gel medium and proteins migrate until they reach the site (or focus) at which the pH is equal to their isoelectric point.Antigens, Fungal: Substances of fungal origin that have antigenic activity.Coccidioides: A mitosporic fungal genus which causes COCCIDIOIDOMYCOSIS.Electrophoresis, Cellulose Acetate: Electrophoresis in which cellulose acetate is the diffusion medium.Polysaccharides, Bacterial: Polysaccharides found in bacteria and in capsules thereof.Carbohydrates: The largest class of organic compounds, including STARCH; GLYCOGEN; CELLULOSE; POLYSACCHARIDES; and simple MONOSACCHARIDES. Carbohydrates are composed of carbon, hydrogen, and oxygen in a ratio of Cn(H2O)n.Immunosorbent Techniques: Techniques for removal by adsorption and subsequent elution of a specific antibody or antigen using an immunosorbent containing the homologous antigen or antibody.Antigen-Antibody Complex: The complex formed by the binding of antigen and antibody molecules. The deposition of large antigen-antibody complexes leading to tissue damage causes IMMUNE COMPLEX DISEASES.Antigen-Antibody Reactions: The processes triggered by interactions of ANTIBODIES with their ANTIGENS.Paraproteinemias: A group of related diseases characterized by an unbalanced or disproportionate proliferation of immunoglobulin-producing cells, usually from a single clone. These cells frequently secrete a structurally homogeneous immunoglobulin (M-component) and/or an abnormal immunoglobulin.gamma-Globulins: Serum globulins that migrate to the gamma region (most positively charged) upon ELECTROPHORESIS. At one time, gamma-globulins came to be used as a synonym for immunoglobulins since most immunoglobulins are gamma globulins and conversely most gamma globulins are immunoglobulins. But since some immunoglobulins exhibit an alpha or beta electrophoretic mobility, that usage is in decline.Electrophoresis: An electrochemical process in which macromolecules or colloidal particles with a net electric charge migrate in a solution under the influence of an electric current.Trichloroacetic Acid: A strong acid used as a protein precipitant in clinical chemistry and also as a caustic for removing warts.Hemagglutination Tests: Sensitive tests to measure certain antigens, antibodies, or viruses, using their ability to agglutinate certain erythrocytes. (From Stedman, 26th ed)Agglutination Tests: Tests that are dependent on the clumping of cells, microorganisms, or particles when mixed with specific antiserum. (From Stedman, 26th ed)Danazol: A synthetic steroid with antigonadotropic and anti-estrogenic activities that acts as an anterior pituitary suppressant by inhibiting the pituitary output of gonadotropins. It possesses some androgenic properties. Danazol has been used in the treatment of endometriosis and some benign breast disorders.Beta-Globulins: Serum proteins with an electrophoretic mobility that falls between ALPHA-GLOBULINS and GAMMA-GLOBULINS.Immunologic Techniques: Techniques used to demonstrate or measure an immune response, and to identify or measure antigens using antibodies.Epitopes: Sites on an antigen that interact with specific antibodies.Ultracentrifugation: Centrifugation with a centrifuge that develops centrifugal fields of more than 100,000 times gravity. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)Myeloma Proteins: Abnormal immunoglobulins characteristic of MULTIPLE MYELOMA.Acholeplasma laidlawii: An organism originally isolated from sewage, manure, humus, and soil, but recently found as a parasite in mammals and birds.Amino Acids: Organic compounds that generally contain an amino (-NH2) and a carboxyl (-COOH) group. Twenty alpha-amino acids are the subunits which are polymerized to form proteins.Electrophoresis, Disc: Electrophoresis in which discontinuities in both the voltage and pH gradients are introduced by using buffers of different composition and pH in the different parts of the gel column. The term 'disc' was originally used as an abbreviation for 'discontinuous' referring to the buffers employed, and does not have anything to do with the shape of the separated zones.Rickettsia typhi: The etiologic agent of murine typhus (see TYPHUS, ENDEMIC FLEA-BORNE).Hypoprothrombinemias: Absence or reduced levels of PROTHROMBIN in the blood.SepharosePrecipitin Tests: Serologic tests in which a positive reaction manifested by visible CHEMICAL PRECIPITATION occurs when a soluble ANTIGEN reacts with its precipitins, i.e., ANTIBODIES that can form a precipitate.Serum Globulins: All blood proteins except albumin ( = SERUM ALBUMIN, which is not a globulin) and FIBRINOGEN (which is not in the serum). The serum globulins are subdivided into ALPHA-GLOBULINS; BETA-GLOBULINS; and GAMMA-GLOBULINS on the basis of their electrophoretic mobilities. (From Dorland, 28th ed)Isoelectric Point: The pH in solutions of proteins and related compounds at which the dipolar ions are at a maximum.Immunoglobulin G: The major immunoglobulin isotype class in normal human serum. There are several isotype subclasses of IgG, for example, IgG1, IgG2A, and IgG2B.Paraproteins: Abnormal immunoglobulins synthesized by atypical cells of the MONONUCLEAR PHAGOCYTE SYSTEM. Paraproteins containing only light chains lead to Bence Jones paraproteinemia, while the presence of only atypical heavy chains leads to heavy chain disease. Most of the paraproteins show themselves as an M-component (monoclonal gammopathy) in electrophoresis. Diclonal and polyclonal paraproteins are much less frequently encountered.Dysentery, Amebic: DYSENTERY caused by intestinal amebic infection, chiefly with ENTAMOEBA HISTOLYTICA. This condition may be associated with amebic infection of the LIVER and other distant sites.Glycoproteins: Conjugated protein-carbohydrate compounds including mucins, mucoid, and amyloid glycoproteins.Species Specificity: The restriction of a characteristic behavior, anatomical structure or physical system, such as immune response; metabolic response, or gene or gene variant to the members of one species. It refers to that property which differentiates one species from another but it is also used for phylogenetic levels higher or lower than the species.Methods: A series of steps taken in order to conduct research.Bence Jones Protein: An abnormal protein with unusual thermosolubility characteristics that is found in the urine of patients with MULTIPLE MYELOMA.Complement Fixation Tests: Serologic tests based on inactivation of complement by the antigen-antibody complex (stage 1). Binding of free complement can be visualized by addition of a second antigen-antibody system such as red cells and appropriate red cell antibody (hemolysin) requiring complement for its completion (stage 2). Failure of the red cells to lyse indicates that a specific antigen-antibody reaction has taken place in stage 1. If red cells lyse, free complement is present indicating no antigen-antibody reaction occurred in stage 1.Actinomyces: A genus of gram-positive, rod-shaped bacteria whose organisms are nonmotile. Filaments that may be present in certain species are either straight or wavy and may have swollen or clubbed heads.Hypergammaglobulinemia: An excess of GAMMA-GLOBULINS in the serum due to chronic infections or PARAPROTEINEMIAS.Chromatography: Techniques used to separate mixtures of substances based on differences in the relative affinities of the substances for mobile and stationary phases. A mobile phase (fluid or gas) passes through a column containing a stationary phase of porous solid or liquid coated on a solid support. Usage is both analytical for small amounts and preparative for bulk amounts.Chromatography, Ion Exchange: Separation technique in which the stationary phase consists of ion exchange resins. The resins contain loosely held small ions that easily exchange places with other small ions of like charge present in solutions washed over the resins.Immunoglobulin kappa-Chains: One of the types of light chains of the immunoglobulins with a molecular weight of approximately 22 kDa.Blood Coagulation Tests: Laboratory tests for evaluating the individual's clotting mechanism.Chromatography, Affinity: A chromatographic technique that utilizes the ability of biological molecules to bind to certain ligands specifically and reversibly. It is used in protein biochemistry. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)Immunoglobulin lambda-Chains: One of the types of light chain subunits of the immunoglobulins with a molecular weight of approximately 22 kDa.Meningitis: Inflammation of the coverings of the brain and/or spinal cord, which consist of the PIA MATER; ARACHNOID; and DURA MATER. Infections (viral, bacterial, and fungal) are the most common causes of this condition, but subarachnoid hemorrhage (HEMORRHAGES, SUBARACHNOID), chemical irritation (chemical MENINGITIS), granulomatous conditions, neoplastic conditions (CARCINOMATOUS MENINGITIS), and other inflammatory conditions may produce this syndrome. (From Joynt, Clinical Neurology, 1994, Ch24, p6)Stanozolol: A synthetic steroid that has anabolic and androgenic properties. (From Martindale, The Extra Pharmacopoeia, 30th ed, p1194)Antigens, Surface: Antigens on surfaces of cells, including infectious or foreign cells or viruses. They are usually protein-containing groups on cell membranes or walls and may be isolated.Antithrombin III Deficiency: An absence or reduced level of Antithrombin III leading to an increased risk for thrombosis.Immunoglobulin A: Represents 15-20% of the human serum immunoglobulins, mostly as the 4-chain polymer in humans or dimer in other mammals. Secretory IgA (IMMUNOGLOBULIN A, SECRETORY) is the main immunoglobulin in secretions.Chemistry: A basic science concerned with the composition, structure, and properties of matter; and the reactions that occur between substances and the associated energy exchange.Antibody Specificity: The property of antibodies which enables them to react with some ANTIGENIC DETERMINANTS and not with others. Specificity is dependent on chemical composition, physical forces, and molecular structure at the binding site.Chemical Phenomena: The composition, conformation, and properties of atoms and molecules, and their reaction and interaction processes.Serum Albumin: A major protein in the BLOOD. It is important in maintaining the colloidal osmotic pressure and transporting large organic molecules.Detergents: Purifying or cleansing agents, usually salts of long-chain aliphatic bases or acids, that exert cleansing (oil-dissolving) and antimicrobial effects through a surface action that depends on possessing both hydrophilic and hydrophobic properties.Complement C3: A glycoprotein that is central in both the classical and the alternative pathway of COMPLEMENT ACTIVATION. C3 can be cleaved into COMPLEMENT C3A and COMPLEMENT C3B, spontaneously at low level or by C3 CONVERTASE at high level. The smaller fragment C3a is an ANAPHYLATOXIN and mediator of local inflammatory process. The larger fragment C3b binds with C3 convertase to form C5 convertase.Blood Platelets: Non-nucleated disk-shaped cells formed in the megakaryocyte and found in the blood of all mammals. They are mainly involved in blood coagulation.Fluorescent Antibody Technique: Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.Complement C4: A glycoprotein that is important in the activation of CLASSICAL COMPLEMENT PATHWAY. C4 is cleaved by the activated COMPLEMENT C1S into COMPLEMENT C4A and COMPLEMENT C4B.Immunoglobulins: Multi-subunit proteins which function in IMMUNITY. They are produced by B LYMPHOCYTES from the IMMUNOGLOBULIN GENES. They are comprised of two heavy (IMMUNOGLOBULIN HEAVY CHAINS) and two light chains (IMMUNOGLOBULIN LIGHT CHAINS) with additional ancillary polypeptide chains depending on their isoforms. The variety of isoforms include monomeric or polymeric forms, and transmembrane forms (B-CELL ANTIGEN RECEPTORS) or secreted forms (ANTIBODIES). They are divided by the amino acid sequence of their heavy chains into five classes (IMMUNOGLOBULIN A; IMMUNOGLOBULIN D; IMMUNOGLOBULIN E; IMMUNOGLOBULIN G; IMMUNOGLOBULIN M) and various subclasses.Immunoassay: A technique using antibodies for identifying or quantifying a substance. Usually the substance being studied serves as antigen both in antibody production and in measurement of antibody by the test substance.alpha 1-Antitrypsin: Plasma glycoprotein member of the serpin superfamily which inhibits TRYPSIN; NEUTROPHIL ELASTASE; and other PROTEOLYTIC ENZYMES.Bacterial Proteins: Proteins found in any species of bacterium.Serotyping: Process of determining and distinguishing species of bacteria or viruses based on antigens they share.Agglutination: The clumping together of suspended material resulting from the action of AGGLUTININS.Cell Membrane: The lipid- and protein-containing, selectively permeable membrane that surrounds the cytoplasm in prokaryotic and eukaryotic cells.alpha-Macroglobulins: Glycoproteins with a molecular weight of approximately 620,000 to 680,000. Precipitation by electrophoresis is in the alpha region. They include alpha 1-macroglobulins and alpha 2-macroglobulins. These proteins exhibit trypsin-, chymotrypsin-, thrombin-, and plasmin-binding activity and function as hormonal transporters.Immunoglobulin Light Chains: Polypeptide chains, consisting of 211 to 217 amino acid residues and having a molecular weight of approximately 22 kDa. There are two major types of light chains, kappa and lambda. Two Ig light chains and two Ig heavy chains (IMMUNOGLOBULIN HEAVY CHAINS) make one immunoglobulin molecule.Antibodies, Bacterial: Immunoglobulins produced in a response to BACTERIAL ANTIGENS.Blood Platelet Disorders: Disorders caused by abnormalities in platelet count or function.Chromatography, DEAE-Cellulose: A type of ion exchange chromatography using diethylaminoethyl cellulose (DEAE-CELLULOSE) as a positively charged resin. (From McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)Rhamnose: A methylpentose whose L- isomer is found naturally in many plant glycosides and some gram-negative bacterial lipopolysaccharides.Antibodies: Immunoglobulin molecules having a specific amino acid sequence by virtue of which they interact only with the ANTIGEN (or a very similar shape) that induced their synthesis in cells of the lymphoid series (especially PLASMA CELLS).Streptococcus: A genus of gram-positive, coccoid bacteria whose organisms occur in pairs or chains. No endospores are produced. Many species exist as commensals or parasites on man or animals with some being highly pathogenic. A few species are saprophytes and occur in the natural environment.Antithrombin III: A plasma alpha 2 glycoprotein that accounts for the major antithrombin activity of normal plasma and also inhibits several other enzymes. It is a member of the serpin superfamily.Transferrin: An iron-binding beta1-globulin that is synthesized in the LIVER and secreted into the blood. It plays a central role in the transport of IRON throughout the circulation. A variety of transferrin isoforms exist in humans, including some that are considered markers for specific disease states.Radioimmunoassay: Classic quantitative assay for detection of antigen-antibody reactions using a radioactively labeled substance (radioligand) either directly or indirectly to measure the binding of the unlabeled substance to a specific antibody or other receptor system. Non-immunogenic substances (e.g., haptens) can be measured if coupled to larger carrier proteins (e.g., bovine gamma-globulin or human serum albumin) capable of inducing antibody formation.Alpha-Globulins: Serum proteins that have the most rapid migration during ELECTROPHORESIS. This subgroup of globulins is divided into faster and slower alpha(1)- and alpha(2)-globulins.Platelet Membrane Glycoproteins: Surface glycoproteins on platelets which have a key role in hemostasis and thrombosis such as platelet adhesion and aggregation. Many of these are receptors.Cell Wall: The outermost layer of a cell in most PLANTS; BACTERIA; FUNGI; and ALGAE. The cell wall is usually a rigid structure that lies external to the CELL MEMBRANE, and provides a protective barrier against physical or chemical agents.Absorption: The physical or physiological processes by which substances, tissue, cells, etc. take up or take in other substances or energy.Latex Fixation Tests: Passive agglutination tests in which antigen is adsorbed onto latex particles which then clump in the presence of antibody specific for the adsorbed antigen. (From Stedman, 26th ed)Trypsin: A serine endopeptidase that is formed from TRYPSINOGEN in the pancreas. It is converted into its active form by ENTEROPEPTIDASE in the small intestine. It catalyzes hydrolysis of the carboxyl group of either arginine or lysine. EC 3.4.21.4.Agar: A complex sulfated polymer of galactose units, extracted from Gelidium cartilagineum, Gracilaria confervoides, and related red algae. It is used as a gel in the preparation of solid culture media for microorganisms, as a bulk laxative, in making emulsions, and as a supporting medium for immunodiffusion and immunoelectrophoresis.Blood Coagulation Factors: Endogenous substances, usually proteins, that are involved in the blood coagulation process.Hemagglutination: The aggregation of ERYTHROCYTES by AGGLUTININS, including antibodies, lectins, and viral proteins (HEMAGGLUTINATION, VIRAL).Immunoglobulin M: A class of immunoglobulin bearing mu chains (IMMUNOGLOBULIN MU-CHAINS). IgM can fix COMPLEMENT. The name comes from its high molecular weight and originally being called a macroglobulin.von Willebrand Factor: A high-molecular-weight plasma protein, produced by endothelial cells and megakaryocytes, that is part of the factor VIII/von Willebrand factor complex. The von Willebrand factor has receptors for collagen, platelets, and ristocetin activity as well as the immunologically distinct antigenic determinants. It functions in adhesion of platelets to collagen and hemostatic plug formation. The prolonged bleeding time in VON WILLEBRAND DISEASES is due to the deficiency of this factor.Macromolecular Substances: Compounds and molecular complexes that consist of very large numbers of atoms and are generally over 500 kDa in size. In biological systems macromolecular substances usually can be visualized using ELECTRON MICROSCOPY and are distinguished from ORGANELLES by the lack of a membrane structure.Peptide Hydrolases: Hydrolases that specifically cleave the peptide bonds found in PROTEINS and PEPTIDES. Examples of sub-subclasses for this group include EXOPEPTIDASES and ENDOPEPTIDASES.Enzyme-Linked Immunosorbent Assay: An immunoassay utilizing an antibody labeled with an enzyme marker such as horseradish peroxidase. While either the enzyme or the antibody is bound to an immunosorbent substrate, they both retain their biologic activity; the change in enzyme activity as a result of the enzyme-antibody-antigen reaction is proportional to the concentration of the antigen and can be measured spectrophotometrically or with the naked eye. Many variations of the method have been developed.Membrane Proteins: Proteins which are found in membranes including cellular and intracellular membranes. They consist of two types, peripheral and integral proteins. They include most membrane-associated enzymes, antigenic proteins, transport proteins, and drug, hormone, and lectin receptors.Fibrinogen: Plasma glycoprotein clotted by thrombin, composed of a dimer of three non-identical pairs of polypeptide chains (alpha, beta, gamma) held together by disulfide bonds. Fibrinogen clotting is a sol-gel change involving complex molecular arrangements: whereas fibrinogen is cleaved by thrombin to form polypeptides A and B, the proteolytic action of other enzymes yields different fibrinogen degradation products.Polyethylene Glycols: Polymers of ETHYLENE OXIDE and water, and their ethers. They vary in consistency from liquid to solid depending on the molecular weight indicated by a number following the name. They are used as SURFACTANTS, dispersing agents, solvents, ointment and suppository bases, vehicles, and tablet excipients. Some specific groups are NONOXYNOLS, OCTOXYNOLS, and POLOXAMERS.von Willebrand Diseases: Group of hemorrhagic disorders in which the VON WILLEBRAND FACTOR is either quantitatively or qualitatively abnormal. They are usually inherited as an autosomal dominant trait though rare kindreds are autosomal recessive. Symptoms vary depending on severity and disease type but may include prolonged bleeding time, deficiency of factor VIII, and impaired platelet adhesion.Solubility: The ability of a substance to be dissolved, i.e. to form a solution with another substance. (From McGraw-Hill Dictionary of Scientific and Technical Terms, 6th ed)Escherichia coli: A species of gram-negative, facultatively anaerobic, rod-shaped bacteria (GRAM-NEGATIVE FACULTATIVELY ANAEROBIC RODS) commonly found in the lower part of the intestine of warm-blooded animals. It is usually nonpathogenic, but some strains are known to produce DIARRHEA and pyogenic infections. Pathogenic strains (virotypes) are classified by their specific pathogenic mechanisms such as toxins (ENTEROTOXIGENIC ESCHERICHIA COLI), etc.Multiple Myeloma: A malignancy of mature PLASMA CELLS engaging in monoclonal immunoglobulin production. It is characterized by hyperglobulinemia, excess Bence-Jones proteins (free monoclonal IMMUNOGLOBULIN LIGHT CHAINS) in the urine, skeletal destruction, bone pain, and fractures. Other features include ANEMIA; HYPERCALCEMIA; and RENAL INSUFFICIENCY.Factor VIII: Blood-coagulation factor VIII. Antihemophilic factor that is part of the factor VIII/von Willebrand factor complex. Factor VIII is produced in the liver and acts in the intrinsic pathway of blood coagulation. It serves as a cofactor in factor X activation and this action is markedly enhanced by small amounts of thrombin.Liver: A large lobed glandular organ in the abdomen of vertebrates that is responsible for detoxification, metabolism, synthesis and storage of various substances.Hot Temperature: Presence of warmth or heat or a temperature notably higher than an accustomed norm.Sodium Dodecyl Sulfate: An anionic surfactant, usually a mixture of sodium alkyl sulfates, mainly the lauryl; lowers surface tension of aqueous solutions; used as fat emulsifier, wetting agent, detergent in cosmetics, pharmaceuticals and toothpastes; also as research tool in protein biochemistry.Teichoic Acids: Bacterial polysaccharides that are rich in phosphodiester linkages. They are the major components of the cell walls and membranes of many bacteria.Mycobacterium: A genus of gram-positive, aerobic bacteria. Most species are free-living in soil and water, but the major habitat for some is the diseased tissue of warm-blooded hosts.Streptococcus mutans: A polysaccharide-producing species of STREPTOCOCCUS isolated from human dental plaque.Goats: Any of numerous agile, hollow-horned RUMINANTS of the genus Capra, in the family Bovidae, closely related to the SHEEP.Microscopy, Electron: Microscopy using an electron beam, instead of light, to visualize the sample, thereby allowing much greater magnification. The interactions of ELECTRONS with specimens are used to provide information about the fine structure of that specimen. In TRANSMISSION ELECTRON MICROSCOPY the reactions of the electrons that are transmitted through the specimen are imaged. In SCANNING ELECTRON MICROSCOPY an electron beam falls at a non-normal angle on the specimen and the image is derived from the reactions occurring above the plane of the specimen.Lipoproteins, HDL: A class of lipoproteins of small size (4-13 nm) and dense (greater than 1.063 g/ml) particles. HDL lipoproteins, synthesized in the liver without a lipid core, accumulate cholesterol esters from peripheral tissues and transport them to the liver for re-utilization or elimination from the body (the reverse cholesterol transport). Their major protein component is APOLIPOPROTEIN A-I. HDL also shuttle APOLIPOPROTEINS C and APOLIPOPROTEINS E to and from triglyceride-rich lipoproteins during their catabolism. HDL plasma level has been inversely correlated with the risk of cardiovascular diseases.Complement System Proteins: Serum glycoproteins participating in the host defense mechanism of COMPLEMENT ACTIVATION that creates the COMPLEMENT MEMBRANE ATTACK COMPLEX. Included are glycoproteins in the various pathways of complement activation (CLASSICAL COMPLEMENT PATHWAY; ALTERNATIVE COMPLEMENT PATHWAY; and LECTIN COMPLEMENT PATHWAY).

Homogeneous pyruvate kinase isolated from yeast by two different methods is indistinguishable from pyruvate kinase in cell-free extract. (1/2055)

In this report, we have compared homogeneous yeast (Saccharomyces cerevisiae) pyruvate kinase to enzyme from cell-free extracts in several different ways: 1) isoelectric focusing of cell-free extracts indicates one peak of pyruvate kinase activity whose isoelectric point is the same as that of the pure enzyme; 2) antibody prepared to the pure enzyme produces a single, fused precipitin line against enzyme in the cell-free extract and pure enzyme; 3) immunoelectrophoresis of cell-free extract produces one precipitin arc which has the same mobility as that of the pure enzyme; and 4) immunoprecipitation of the pure enzyme from cell-free extract with subsequent solubilization in 1% sodium dodecyl sulfate and electrophoresis on sodium dodecyl sulfate-polyacrylamide gels produces a single protein band attributable to pyruvate kinase which co-migrates with the purified enzyme. Within the limits of the sensitivity of the methods employed, we conclude that the homogeneous pyruvate kinase prepared from yeast lysed either by Manton-Gaulin homogenization (Aust, A., Yun, S.-L., and Suelter, C. (1975) Methods Enzymol. 42, 176-182) or by toluolysis (Yun, S.-L., Aust, A.E., and Suelter, C.H. (1977) J. Biol. Chem. 251, 124-128) is identical with pyruvate kinase in cell-free extract.  (+info)

Enzymatic and immunological characterization of the Mycobacterium fortuitum complex. (2/2055)

The arylsulfatase isozymes of Mycobacterium fortuitum, M. peregrinum, M. chelonei subsp. chelonei, and M. chelonei subsp. abscessus were examined to determine the isozymal and immunological relationship among the members of the M. fortuitum complex. Cell extracts were subjected to electrophoresis on agarose and polyacrylamide gel, and arylsulfatase activity was localized using beta-naphthyl sulfate as substrate. Unique zymograms were produced for M. fortuitum, M. peregrinum, and M. chelonei which were characteristic for each species. The immunological relationship among the sulfatases was assayed by using immunodiffusion and immunoelectrophoresis followed by sulfatase staining for the enzyme. One of the isozymes of M. fortuitum and M. peregrinum cross-reacted, showing immunological identity. Antisera to sulfatases of M. fortuitum and M. peregrinum did not react with sulfatases of M. chelonei. The characterization of sulfatase isozymes in extracts of organisms in the M. fortuitum complex suggests the division of the M. fortuitum complex into two species, M. fortuitum and M. chelonei, with subspecies designations.  (+info)

The phenoloxidases of the ascomycete Podospora anserina. Structural differences between laccases of high and low molecular weight. (3/2055)

In order to investigate the extent of the relationship between the three copper-containing glycoproteins, laccases I, II and III (Mr70000, 80000 and 390000 respectively) of Podospora anserina, the following experiments were carried out on laccases II and III: (a) determination of amino acid composition; (b) determination of N-terminal and C-terminal amino acid; (c) determination of sugar composition; (d) dissociation studies on native and denatured laccases and also after removal of copper from the enzymes; (e) digestion of the carbohydrate moieties with the aid of glycosylhydrolases. A comparison between the results of these experiments and data previously obtained with laccase I allows the following conclusions to be drawn. 1. Laccases II and III are not identical. 2. Neither of these low molecular weight laccases are as complete molecules subunits of the oligomeric laccase I. 3. The possibility of partial identity of amino acid sequences of laccases I and III can not be excluded. 4. Laccase II possibly consists of subunits of Mr37000 whereas laccase III does not. 5. Digestion of 50% of the carbohydrate content leads to complete loss of serological specificity (serological reaction and cross reaction). This finding is discussed with regard to the possible role of the carbohydrate moiety as antigenic determinants and thus as the reason for the immunological relationship. As a consequence, at least three independent structural genes for laccases must be assumed.  (+info)

Inhibition of human seminal fluid DNA polymerase by an IgG fraction of seminal plasma from vasectomized men. (4/2055)

Immunoglobulin G (IgG) was isolated from ejaculates of intact and vasectomized men by precipitation with ammonium sulphate and DEAE-cellulose ionexchange chromatography. Velocity centrifugation revealed that all of the IgG from intact males was 7S protein while less than 40% of the seminal IgG of vasectomized men cosedimented with the 7S marker; the remaining, immunologically unidentifiable, protein was considerably smaller and heterogeneous in size. Only the 7S IgG from the post-vasectomy ejaculates inhibited the activity of a DNA polymerase from the seminal fluid of an intact male. These results suggest that formation of antibody reactive with the seminal fluid DNA polymerase is one manifestation of a vasectomy-associated autoimmune response in man.  (+info)

Complement activity in middle ear effusions. (5/2055)

Evidence for complement utilization in middle ear fluids (MEF) from patients with otitis media with effusion was sought. It was found that cleavage products of C3, C4 and Factor B could be demonstrated immunochemically in MEF, and that native C3 was present in much lower concentrations than other proteins, relative to their serum concentrations. Haemolytic assays for C1-C5 showed that early complement components are inactivated in MEF. Potential mechanisms for complement utilization in MEF are discussed.  (+info)

Enterobacterial common antigen: isolation from Shigella sonnei, purification and immunochemical characterization. (6/2055)

In the studies presented the effective procedure of isolation and purification of enterobacterial common antigen from Shigella sonnei has been elaborated. The method is based on sonification of bacterial suspension in the presence of lysozyme and EDTA and subsequent extraction of the pellet with boiling water. The crude extract of common antigen was purified by fractionation with ethanol and chromatography on silica gel and Sephadex LH-20. The comparison of several extraction procedures of enterobacterial common antigen from Shigella sonnei proved that the method described above is most effective. The purified enterobacterial common antigen preparation obtained preserved full biological activity: antigenicity (precipitation and activity in enzyme-linked immunosorbent assay), immunogenicity in rabbits, ability to coat erythrocytes (passive hemagglutination) and inhibitory activity in passive hemagglutination. The pure enterobacterial common antigen was identified to 90% as a polymer of N-acetyl-D-mannosaminuronic acid and N-acetyl-D-glucosamine (2:1, molar ratio), O-acetylated and containing 3.2% fatty acids (C16:0 and C18:1, not oleic). It contains 5.3% nitrogen, less than 4% protein, less than 0.5% phosphorus and less than 1.6% neutral sugar; glycerol and RNA were not found in the preparation.  (+info)

Immune response in the garter snake (Thamnophis ordinoides). (7/2055)

Garter snakes (Thamnophis ordinoides) were immunized with hen egg albumin, human gamma-globulin and Keyhole limpet haemocyanin in Freund's adjuvant. Antibody was consistently detected by radioimmunoelectrophoresis and in three different gamma- and beta-globulin precipitin lines called IgM (approximately or equal to 20S), Ig-1 (approximately or equal to 9S) and Ig-2 (approximately or equal to 8-5S). Early antibody (day 31 after immunization) was frequently Ig-M whereas Ig-2 and especially Ig-1 were detectable for the longest duration (992 days). After immunization with antigen in Freund's adjuvant, Ig-1 serum concentration showed the greatest increase, from almost undetectable levels to the most prominent immunoglobulin in immune serum.  (+info)

Plant microbody proteins. Purification and glycoprotein nature of glyoxysomal isocitrate lyase from cucumber cotyledons. (8/2055)

1. Isocitrate lyase from cotyledons of cucumber seedlings (Cucumis sativus) has been purified 100-fold. Two methods of preparing the soluble glyoxylate cycle enzyme are described: an elaborated method which used crude extracts of cucumber cotyledons, and another procedure which started with purified glyoxysomes from 4-day-old cotyledons and included a separation of glyoxysomal matrix enzymes by zonal centrifugation. The product behaved as a single species when tested by (a) polyacrylamide gel electrophoresis in the presence of dodecyl sulfate, (b) zonal centrifugation, and (c) double immunodiffusion against rabbit antibody to isocitrate lyase. 2. Isocitrate lyase of cucumber glyoxysomes exhibited a molecular weight of 255,000 and was composed of four apparently identical subunits of Mr 64,000. An isoelectric point of 5.9 was determined. 3. It was shown that isocitrate lyase is a glycoprotein, (a) by Schiff stain on polyacrylamide gels, (b) by periodate oxidation of the enzyme, subsequent reduction with NaB[3H]4 and electrophoretic analysis of the labelled glycoprotein, and (c) by incorporation of [3H]glucosamine in vivo into a protein which could be precipitated with antibodies to isocitrate lyase and revealed a 64,000-Mr band upon electrophoresis.  (+info)

*Immunoelectrophoresis

Crossed immunoelectrophoresis is also called two-dimensional quantitative immunoelectrophoresis ad modum Clarke and Freeman or ... Rocket immunoelectrophoresis is one-dimensional quantitative immunoelectrophoresis. The method has been used for quantitation ... fused rocket immunoelectrophoresis ad modum Svendsen and Harboe, affinity immunoelectrophoresis ad modum Bøg-Hansen. Agarose as ... crossed immunoelectrophoresis (two-dimensional quantitative immunoelectrophoresis ad modum Clarke and Freeman or ad modum ...

*Counterimmunoelectrophoresis

Immunoelectrophoresis Electrophoresis Ling IT.; Cooksley S.; Bates PA.; Hempelmann E.; Wilson RJM. (1986). "Antibodies to the ... http://medical-dictionary.thefreedictionary.com/counter+immunoelectrophoresis Sherris, John C.; Ryan, Kenneth J.; Ray, C. L. ( ...

*Gel electrophoresis of proteins

This is the text of choice for immunoelectrophoresis. Discontinuous native protein gel electrophoresis Protein purification ... or as immunoelectrophoresis. Traditionally, two classes of blood proteins are considered: serum albumin and globulin. They are ... or gel electrophoresis Immunoelectrophoresis Immunofixation Native gel electrophoresis QPNC-PAGE Paraprotein Fast parallel ... immunoelectrophoresis, counterelectrophoresis, and capillary electrophoresis. Each method has many variations with individual ...

*Major urinary proteins

Roy AK, Neuhaus OW (1966). "Identification of rat urinary proteins by zone and immunoelectrophoresis". Proceedings of the ...

*Affinity electrophoresis

Immunoelectrophoresis Ream JA, Lewis LK, Lewis KA (October 2016). "Rapid agarose gel electrophoretic mobility shift assay for ... "rocket immunoelectrophoresis", affinity electrophoresis may be used as an alternative quantification of the protein. Some of ...

*Antigen-antibody interaction

Sophisticated applications include ELISA, enzyme-linked immunospot (Elispot), immunofluorescence, and immunoelectrophoresis. ... "Identification of Cytosolic Antigens from GW-39 Adenocarcinoma Cells by Crossed Immunoelectrophoresis and Immunofluorescence". ...

*Barbital

Solutions of sodium barbital have also been used as pH buffers for biological research, e.g., in immunoelectrophoresis or in ... Monthony, JF; Wallace, EG; Allen, DM (Oct 1978). "A non-barbital buffer for immunoelectrophoresis and zone electrophoresis in ...

*Gerald Penn

... is a clinical immunologist, and a pioneer in the field of clinical immunoelectrophoresis. He was a student of Henry ...

*Tree nut allergy

Goetz, DW (July 2005). "Cross-reactivity among edible nuts: double immunodiffusion, crossed immunoelectrophoresis, and human ...

*Hypoallergenic dog breed

"Characterization of extract of dog hair and dandruff from six different dog breeds by quantitative immunoelectrophoresis. ...

*Immunofixation

... as immunoelectrophoresis, takes place in two steps: The first step is identical for both techniques. It consists ... fr:Immunofixation Immunoelectrophoresis MedlinePlus Encyclopedia 003543 - "Immunofixation - serum" MedlinePlus Encyclopedia ...

*George Siber bibliography

Scheifele, DW; Ward, JI; Siber, GR (Dec 1981). "Advantage of latex agglutination over countercurrent immunoelectrophoresis in ...

*Haemophilus influenzae

Countercurrent immunoelectrophoresis has been shown to be an effective research diagnostic method, but has been largely ...

*Auchterlonie

Ouchterlony O (1970), Handbook of Immunodiffusion and Immunoelectrophoresis, Ann Arbor, MI: Ann Arbor-Humphrey Science ...

*Academy of Natural Sciences of Drexel University

Allozymes, DNA-DNA hybridization, immunoelectrophoresis, restriction site analyses of mitochondrial DNA and serology were used ...

*Barbiturate

Sodium barbital and barbital have also been used as pH buffers for biological research, e.g., in immunoelectrophoresis or in ...

*Waldenström's macroglobulinemia

Immunoelectrophoresis and immunofixation studies help identify the type of immunoglobulin, the clonality of the light chain, ...

*Electrophoresis

Dielectrophoresis Electroblotting Gel electrophoresis Gel electrophoresis of nucleic acids Immunoelectrophoresis Isoelectric ...

*Agarose gel electrophoresis

Gel electrophoresis Immunodiffusion, Immunoelectrophoresis SDD-AGE Northern blot SDS-polyacrylamide gel electrophoresis ...

*Agarose

... is also used widely for a number of other applications, for example immunodiffusion and immunoelectrophoresis, as the ...

*Cryoglobulinemia

The precipitated cryoglobulins are examined by immunoelectrophoresis and immunofixation to detect and quantify the presence of ...

*Antibody

... immunoelectrophoresis, and magnetic immunoassay. Antibodies raised against human chorionic gonadotropin are used in over the ...

*Methods to investigate protein-protein interactions

Pull-down assays are a common variation of immunoprecipitation and immunoelectrophoresis and are used identically, although ...

*Agar

... ose gel electrophoresis Algaculture Alginic acid Asepsis Carrageenan Immunodiffusion Immunoelectrophoresis Lima bean agar ...

*IEP

... can refer to: Science and technology Immunoelectrophoresis, electrophoresis based separation methods for proteins ...
Looking for immunoelectrophoresis? Find out information about immunoelectrophoresis. A combination of the techniques of electrophoresis and immunodiffusion used to separate the components of a mixture of antigens and make them visible by... Explanation of immunoelectrophoresis
The WS-1B rocket, with a new high-performance rocket motor and warhead, reaches a maximum speed of Mach 5.2 and maximum flight altitude of 60km, giving a minimum firing range of 60km and a maximum firing range of 180km. The probability deviation is between 1% and 1.25%. The WS-1B rocket is longer than the WS-1, with length 6.37m but the same diameter. The take-off weight is 725kg with a 150kg warhead.. The free rocket consists of the warhead and fuse, an FG-43 rocket motor and the tail section. The FG-43 rocket motor is a single chamber, solid rocket motor with an advanced hydroxy-terminated polybutadine (HTPB) composition rocket propellant.. Two types of warhead can be fitted on the WS-IB rocket, a ZDB-2 blasting warhead or an SZB-1 submunition, which are selected according to the characteristics of the target.. The ZDB-2 blasting warhead is loaded with steel balls and prefabricated fragments. The SZB-1 submunition warhead provides an effective high power weapon against massed tanks. When the ...
Rocket Labs Electron commercial satellite launcher lifted off from New Zealand on its debut test flight Thursday and soared into space on its maiden mission.. The 55-foot-tall (17-meter) launcher did not reach orbit as intended, but Rocket Lab said the Electron successfully flew through the boosters first stage burn, stage separation, second stage ignition and payload fairing separation.. The Electron is designed to loft CubeSats and other small satellites into orbit, and Rocket Lab already has launch contracts with NASA and several commercial customers.. These video clips provided by Rocket Lab show multiple views of the Electron lifting off from its commercial base in New Zealand, plus imagery captured by an on-board camera showing first stage separation, second stage ignition and the release of the rockets payload fairing.. Read our full story on the test launch for details.. Video credits: Rocket Lab. ...
Let V be the total volume of the rocket. Let Vw be the volume of water in the rocket, and Va be the volume of air. Let Vai and Vwi be the inital volumes of air and water, at the time of launch. Let p be the gauge pressure inside the rocket (i.e. the pressure above atmospheric). Let pi be the pressure at the time of launch. Well assume static fluids at all times (this is not really true, but at low escape velocities it is inconsequential). Let m be the mass of the rocket (well assume that the water is the only important mass -- the mass of the air and rocket body will be considered negligible). Let o be the exhaust hole area at the bottom of the rocket, which is considered a plane hole. Let v be the exit velocity of the water. Let h be the height of the water in the rocket ...
rocket definition: The definition of a rocket is a long circular device that is launched into the air. (noun) An example of a rocket is what helps launch a guided missile into space. An example of a rocket is a firecracker. ...
Shiver of light - (merry gentry 9) (Heftet) av forfatter Laurell K. Hamilton. Pris kr 119. Se flere bøker fra Laurell K. Hamilton.
Rabbit anti-sheep IgG (H/L) is specific for sheep IgG heavy and light chains. Immunoelectrophoresis resulted in a single precipitin arc ag
The Electron rocket, built with 3D-printed engines and a mostly composite construction, is designed for rapid manufacture and a high launch rate. The relatively small launch vehicle, only 56 feet tall, is catered to the growing demand for a rocket to place smaller satellites in orbit. Ultimately, Rocket Lab wants to ramp up to about 50 Electron launches per year from its private spaceport in New Zealand. Before it can do that, however, Rocket Lab needs to complete its vehicle testing program, which could continue with a launch as early as Friday night. ...
model rocket launch pad for sale - 25 - model rocket launch pad wholesalers & model rocket launch pad manufacturers from China manufacturers.
Rocket Lab successfully launched a rocket from the Hawkes Bays Mahia Peninsula on Sunday, sending its Electron satellite into orbit. The triumph means Rocket Lab will soon be ready to forge ahead in the commercial space industry. To discuss the significance of the launch were joined by Nick Rattenbury, an astrophysicist at the University of Auckland.
Rocket engines. View of the nozzles of rocket engines on the first stage of a Russian Proton-M launcher. The Proton-M has six RD-253 first-stage engines developing a total of 10.5 MN (mega-newtons) of thrust. He rocket has a payload capacity of 22 tonnes to low-earth orbit or 3.5 tonnes to geostationary orbit with the Briz upper stage. Photographed at the Baikonur space centre in Kazkhstan in 2007. - Stock Image C007/3258
How to Make a Rocket. Rockets illustrate Newtons Third Law of Motion: For every action, there is an equal and opposite reaction. The first rocket may have been a steam-propelled wooden pigeon invented by Archytas of Tarentum in the...
When the Rockets needed to get something going offensively late in Sundays game, coach Kevin McHale decided to throw something different at the Trail Blazers. The tactic worked, as the Rockets outscored the Trail Blazers 33-21 in the final quarter - Harden had 17 of those, and Lin had nine. The three guards did provide an offensive spark, but their defense also was key to the victory. In the last several wins for the Rockets, multiple lineups have worked in their favor.
How is Multiple Launch Rocket System Fire Direction System abbreviated? MLRSFDS stands for Multiple Launch Rocket System Fire Direction System. MLRSFDS is defined as Multiple Launch Rocket System Fire Direction System very rarely.
Stomp on the launch pad and generate a blast of air that propels a rocket 200 ft into the air. Rocket features a rounded nosecone made of foam for safe launches and landings. Kit includes 4 rockets and a launch pad.
A long time ago, in a galaxy far far away (okay, really it was only over here a few years ago), I wrote an article about plot. The past couple days, Ive been reading Laurell K. Hamiltons latest book (I use that term loosely, based on the fact that it was produced by a publishing company,…
Vassilis Spanoulis got a chance when Kirk Snyders right hand was broken Monday night, then made enough of it to extend his playing time well into the fourth quarter of a 97-90 victory over the Knicks. More than anything, Spanoulis played 13 minutes without the waves of turnovers or defensive gambles that filled his preseason minutes. Van Gundy said Spanoulis is guaranteed increased playing time as long as he can keep his turnovers to a minimum. Theres that fine line between aggression - and he needs to play aggressively on offense - and being careful with the ball, especially as we struggle through this turnover period were going through. [...] his quickness could help offset the trend toward playing two smaller, quick guards, a backcourt style that has given the Rockets trouble. To help negate his turnover tendencies, the Rockets will likely keep Spanoulis at shooting guard, rather than the point guard position he played in the preseason. Shy about shootingWith a shot that is a work in
12 of Rocket League's Battle-Cars have been miniaturized into little pull-back racers! Each one comes packaged up inside a Rocket Ball, which when opened will reveal the surprise of which racer you've got. Select cars include DLC code for in-game content!
Lockout, shmockout. Big deal. Season or not, the Rockets want you to know that buying season tickets is still a safe investment. Fans wont lose a penny if any games are missed. The Rockets are offering two plans for season ticket holders. Season tic… … ...
CLEVELAND -- All-Star guard James Harden fired in 37 points in the Houston Rockets' 118-111 victory over the Cleveland Cavaliers on Saturday at Quicken Loans Arena. He hit the Cavaliers with a 17-point barrage in the third quarter, including a rare four-point play. He helped the Rockets post their
Dnepr rocket also dubbed Satan has blast off into earths orbit carrying over 30 satellites from 17 countries, including the US. The rocket itself is of Russia-Ukraine make.
Specialist training in the operation of advanced rocket launchers. 2% bonus per level to the rate of fire of modules requiring Rocket Specialization ...
Goat anti Mouse IgG antibody recognizes both the heavy and light chains of mouse IgG. Immunoelectrophoresis revealed a single precipitin a
Hello, Im completely new to shooters that require anything besides hitscan aim. Because of this I find aiming with rockets incredibly hard. I cant seem to hit much and most of the hits feel like random luck because I dont really know what Im doing. The only tip Ive gotten so far is to only use rockets if you can see enemys feet. What other sort of stuff should I know about? When do I aim at the floor besides them and when do I go for a direct hit? When should I jump in a rocket vs. rocket fight? How fast should I be shooting? Is there a generally accepted "better" crosshair to use with rockets, I always seem to aim too far up. And as if thats not hard enough - whats the logic behind dodging? What kind of positioning should I utilize? Do people jump to dodge? How to figure out your opponent while constantly getting hit? I really wish Reflex had bots and aim maps, would make practising a lot more enjoyable. Thanks ...
Many kids don't like dark rooms. Time to add some space-age whimsy (and light) to their wall with this Red Rocket. Remote controlled lights and sounds will help them not be so scared of the dark.
Michael Strano set off hydrogen-fueled rockets as a kid. Today his lab is developing nanosensors, bionic plants, nanoscale ice wires, and a brand-new source of energy.
http://www.serebii.net/anime/NextOn/585.jpgTeam Rocket Disband!? Travelling towards the next town, Team Rocket encounter an old friend whom has set up a Ramen shop in their honour. Seeing how successful he has been since he quit Team Rocket, Jessie & James start considering whether their vocation has taken them as far as its going to. Meanwhile, Ash & Co. near to Team Rocket, enter a valley thats inhabited by an Alternate Coloured Metagross... Visit The Episode Guide Discuss
The amount of data sent to or from mobile gadgets around the world annually will rocket to hit the equivalent of 33 billion full-length DVD films by the year 2016, computer networking titan Cisco forecast on Tuesday.
With Tuesdays addition of Omer Asik and the signing of point guard Jeremy Lin last week, the overhaul of the roster has brought the Rockets the type of young players at key positions they believe they can build around. … ...
Advertise on Rocket Fuel? See their Reviews, Cost, Contacts, Competitors. Pricing models include % of Media Spend, CPA, CPC, CPM, CPV supporting Desktop Display, Email, Mobile Display, Social, Desktop Video, Mobile Video channels
Trouvez tous les livres de Wood, Ingrid; Como, Denise - A Breeders Guide to Genetics: Relax, Its Not Rocket Science. Sur eurolivre.fr,vous pouvez commander des livres anciens et neufs.COMPARER ET acheter IMMÉDIATEMENT au meilleur prix. 9781414024776
https://youtu.be/SK-1iXJBI3U Students had fun today with flying rocket experiment. Conducted this activity to introduce reaction of acid with carbonate. Add sprite in film canister, to about 3/4. Add 3 spatulaful of baking soda on the cap. Place the cap over the film canister and shut it tight. Invert the film canister. Step away and watch…
Deska Rocket Science norských Apoptygma Berzerk už je venku sice hezkých pár týdnů, ale o její recenzi z pera Pavla Zelinky jsme vás nemohli a nechtěli ochudit.
A question came to me via the Ask the Expert service that Reading Rockets provides. With the teachers permission, Im including it here to get your opinion. Question:
A question came to me via the Ask the Expert service that Reading Rockets provides. With the teachers permission, Im including it here to get your opinion. Question:
WALTHAM, Mass., July 26, 2017 /PRNewswire/ -- Rocket Software, a global technology provider specializing in modernization and optimization, has augmented the...
On May 5, 2017, scientists will launch a sounding rocket 200 miles up into the atmosphere, where in just five minutes, it will take 1,500 images of the sun. The NASA-funded RAISE mission is designed to scrutinize split-second ...
A communications satellite flying piggyback aboard a SpaceX Falcon 9 rocket fell out of orbit and burned up in the atmosphere following a problem during liftoff
HOUSTON - Kevin Durant scored 32 and the Warriors used a big third quarter to build a huge lead and coast to their sixth straight victory, 125-108 Friday over the Rockets. In a matchup of two o
, Mouse IgG2a antibody, GTX29163, Applications: ELISA, ID, IE, WB; ELISA, Immunodiffusion, Immunoelectrophoresis, Western Blot (WB); CrossReactivity: Mouse
The other day I walked outside to find my car covered in pollen, so clearly we are entering the peak season for seasonal allergies. The question I always address at this time of year is how to reduce allergy symptoms naturally. First, start with overall health and diet. For instance, people who consume diets higher…
How do we live longer healthier lives? Is it inevitable that our cells will stop functioning properly and we will get chronic degenerative diseases like diabetes, heart disease, and dementia? My goal is to support my body now so that I can delay the
Hi friends we are here again to present to you the new Sci-Fi Dungeon. Because your suggestions, we have decided to offer it to you in a new format, with more combination possibilities. This time, on one hand, we´ve made it easier for you to get it; on the other hand, you have three different ways to do it each with their own kickstarter campaign for funding ...
One of the main problems with diarrhoea is that its so bloomin difficult to spell. If you have trouble remembering how to spell Diarrhoea, remember this handy phrase: Dashing - In - A - Rush? - Really - Hurry - Or - Else - Accident.
The age of imaginary friends has entered my calm household. Who you might ask? Why Sophie has added an imaginary friend--namely Rocket from Little Einsteins on Playhouse Disney. There he is in that picture. No, not any of the smiling children (although Sophie assures me that June is her friend, too, but apparently not close enough for Sophie to let her in the house) but, the rocket ship ...
Engineers behind the British Bloodhound Land Speed Record (LSR) vehicle are turning their eyes to hydrogen power for their next record attempt.
2016年初夏に新雑誌が誕生します。詳細はここで発表します。夏、宇宙、音楽、美しいもの、をテーマにまったく新しいコンセプトの雑誌になります。たくさんの人に読んでいただけるよう、気合いを入れて企画中です。
I have had this happen once.. Unfortunately ended up losing the rocket, but they promptly replaced the case and the motor - also a G138. Burned through...
Dont worry, training your dog does not need to be rocket science, in fact, even the most challenging of puppies can respond to the right type of training. .
http://www.serebii.net/anime/NextOn/481.jpgGood Luck, Starly! On their way to the next town, Ash decides to train his Starly ready for the next gym. However, while training, Starly gets caught up with many flying Pok mon as they get trapped by Team Rocket. Can Ash rescue his feathered friend? Visit The Episode Guide Discuss
An extract of mixed dog hair and dandruff from six different dog breeds (alsatian, boxer, collie, poodle, and long-haired and short-haired dachshund) was obtained by mild extraction, centrifugation, dialysis and freeze-drying. Extract of hair and dandruff from the individual dog breeds was obtained …
Define rise like a rocket and fall like a stick. rise like a rocket and fall like a stick synonyms, rise like a rocket and fall like a stick pronunciation, rise like a rocket and fall like a stick translation, English dictionary definition of rise like a rocket and fall like a stick. n. 1. a. A rocket engine. b. A vehicle or device propelled by one or more rocket engines, especially such a vehicle designed to travel through space. 2.
Carl-Bertil Laurell (born 28 June 1919 in Uppsala, dead 18 September 2001 in Malmö) was a Swedish medical doctor and researcher. Laurell was Professor of clinical chemistry at Lund University. He named the blood plasma protein Transferrin, and discovered that an inherited lack of Alpha 1-antitrypsin could lead to emphysema. In 1976, he was made a member of the Royal Swedish Academy of Sciences. Carl-Bertil Laurell, biographical entry in Nationalencyclopedin (in Swedish ...
The defined antibody specificity is directed to albumin as tested against cat sera. In immunoelectrophoresis and double radial immunodiffusion (Ouchterlony), using various antiserum concentrations against appropriate concentrations of the immunogen, a single characteristic precipitin line is obtained which shows a reaction of identity with the precipitin lines obtained against cat serum and the purified albumin. As precipitating antiserum to identify or measure cat albumin by a variety of immunodiffusion techniques, including immunoelectrophoresis, single and double radial immunodiffusion (Mancini, Ouchterlony) and electroimmunodiffusion (Laurell). It has not been tested for use in nephelometry, ELISA or immunochemistry, but this does not exclude such use if proper controls are included ...
This list provides a one-stop forum on homemade rockets powered by compressed air and water, and related topics. Water rockets are typically made from discarded plastic soft drink bottles, partially filled with water, pumped with air to high pressure, and released. Single stage rockets can reach 500 feet, with top speeds over 100 miles per hour! Advanced rockets may use other materials, other gases, or no water. There are no rules, except to learn while having fun! The list has a history dating back to 1997, but moved from a private service to Yahoo in 2003 with 170 members and 17,000 messages in the archives. The old home page may be still available at http://cjh.polyplex.org/rockets/list/ Postings by new members are moderated (must be approved), until the new member is promoted to full posting status. This helps keep the group free of spam - spammers are quickly banned. Sorry for any inconvenience this might cause.
... : Have fun with this promotional item which is like a fun sling shot. Foam rocket. Pull back, take aim and let them fly! Improved for better performance, better looks and most important of all, better durability. Let out your frustrations by scoring a touchdown with this mini football rocket! Made of lightweight stress foam, this fun little toy is great for the beach, park or football field. Even the most serious business people need a toy to play with! Decorate this item in a variety of colors with a printed name or logo and theyll remember your business forever. Hand out this stress reliever at your next event and send your brand visibility sky-high!
The Kelowna Rockets defeated the Vancouver Giants 5-4 in overtime on Friday night at Prospera Place in Kelowna.. Rockets Captain Cal Foote set up Carsen Twarynski for the winner in overtime as Kelowna came from behind to earn the win.. James Porter stopped 24 of the 28 shots he faced in the winning effort. His record improves to 6-2-2-0 on the season while overall the Rockets improved to 9-6-2-1.. The Rockets opened the scoring as Gordie Ballhorn (2) ripped home a power play marker at 13:53 of the first. Ty Ronning(16) and Tyler Benson(3) responded for the Giants and Vancouver took a 2-1 lead into the middle frame.. Kelowna trailed by one until late in the period when Leif Mattson (6) evened things at 2-2. The Rockets and Giants remained tied after 40 minutes.. The Giants regained a one goal lead but it was short lived as Kole Lind (10) scored a shorthanded marker to knot things up once again. Conner Bruggen-Cate (2) pulled Kelowna ahead on the power play at 11:46, but the Giants kept the ...
i. Reaction of identity: Antigens in two wells are identical (Agl and Ag2). So the reaction with the antibody results in a precisely similar precipitin lines. The precipitin lines dont cross, but form a continuous line. Fusion of the two precipitin lines indicates that the antibody is reacting with epitopes commonly present on the two antigens. ii. Reaction of non-identity: Two non-identical antigens (Agl and Ag3) are in the antigen wells while the antibody well has antibodies to both the antigens. The two antigen-antibody precipitin lines formed differ from each other. Hence the two precipitin lines completely cross (intersect) each other. iii. Reaction of partial identity: The antigenic determinants in the antigens of two wells are partially shared (Agl and Agla). Hence the antibody reacts with both the antigens and forms lines that do not form a complete cross. The precipitin line crosses in only one direction. The extended precipitin line is referred to as a spur. The spur indicates that ...
The X 4 is an antiaircraft rocket designed by the Germans to be launched from planes. It was manufactured and reported to have been successfully tested, but never reached the point of combat operation. It is a wire-controlled, rocket-propelled, fin-stabilized missile fitted with a proximity fuzed warhead. The propulsion system is a bi-fuel rocket. Stabilization is achieved by means of four large fins fitted to the body of the rocket, and four smaller fins fitted to the tail. The smaller fins bear solenoid-operated control surfaces through which two-dimensional directional control is achieved. These are operated from the parent aircraft by means of a control unit and two insulated wires leading to the rocket. These wires are about 3 3/4 miles long.. Precise information about the warhead and fuzing system has not so far been recovered. The warhead consists of an uncased moulded grain of dinitroglycol-based explosive which depends on high blast effect. The fuze is a combination of acoustic ...
Launch of Russian Proton Rocket with British Inmarsat 5-F2 Sat, Proton M rocket explosion July 2 2013 slow motion full HD, Proton M rocket explosion, NEW VIDEO 1, from Astrium team, July 2, 2013, Rocket Profile - Proton
The Big Betty is the largest and tallest level 1 model rocket kit available! With a maximum altitude of 800 feet this rocket is simply impressive in flight
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Rocket Fuel is an indica-dominant cannabis strain that emits a scent that’s not unlike its name. Both scent and taste are strongly diesel-like with undertones of sweet spices. Cured nuggets of Rocket Fuel are small, round, and dense, and are colored deep green with long burnt orange pistils....
Tonight (Jan. 19), the private spaceflight company Rocket Lab will once again attempt a second test launch of its small-scale Electron rocket.
... com - Listen to Free Music from She Swings Blue Vol 1 Joint is Jumpin by The Rhythm Rockets. ARTISTdirect.com is where music and people connect.
Rockets coach Kevin McHale, whose 23-year-old daughter Alexandra died on Nov. 25 from complications related to Lupus, returned from personal leave and coached the team in Saturdays loss to the Mavericks … Tonights game is the second between the teams in four days. The Spurs defeated the Rockets at the AT&T Center on Friday, 114-92 … Teams split last seasons series 2-2, each winning both games on respective home courts.. - Mike Monroe. ...
By Steven Apfelbaum Dames rocket has escaped from gardens and is rapidly invading the surrounding landscapes. Left unchecked, this beautiful, yet lethal plant will wreak havoc on the natural environment, threatening the survival of native plants and degrading habitat and water quality. Dames rocket...
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Hello=all, I am very happy that Rama ruleset is confirmed for 1.2. This will mean big things for duel, however it brings up an issue for the atdm gamemode. In atdm you tend to have rounds that last a while, and you need more ammo than duel, especially when youre playing a bigger game like 4v4 for example. Ive found through playing that the ammo limits with the current ruleset are pretty good with atdm, however the new rama ruleset ammo caps wont give players enough options, especially with only 16 rockets. To fix this, I think voting on the arena mutator should change the max ammo as well. So, what should these new ammo values be? Here Ive gathered the values of ammo caps for different versions of reflex and cpma: Current ruleset ammo caps: grenades - 15 rockets - 25 ion - 150 bolt - 10 plasma - 120 Rama ruleset ammo caps: grenades - 10 rockets - 16 ion - 150 bolt - 10 plasma - 100 CPMA CA ammo caps: grenades - 15 rockets - 50 lg - 150 rail - 15 plasma - 50 OLD Reflex ammo caps: grenades - ...
There are 669 calories in 1 serving (8 oz) of Johnny Rockets Half Rings & Half Fries, without sauce. Youd need to walk 175 minutes to burn 669 calories. Visit CalorieKing to see calorie count and nutrient data for all portion sizes.
Along with the protection against weather, this mixture of metals provide an amazing heat insulator, reducing the temperature of the rocket on re-entry of the atmosphere by about 200 degrees Fahrenheit ...
View Notes - sysprob3 from AERO 16.01 at MIT. Unified Systems Problem 3 WATER BOTTLE ROCKET PERFORMANCE ANALYSIS Learning Objectives: After completing this systems problem you will have: • Applied
Luis Scola, F/C, HOU Now you know why Scola was such a popular pick among experts in the middle rounds. Through two games, Scola is averaging 27.0 points and 16.0 rebounds. Obviously those averages aren t sustainable unless your name is Wilt Chamberlin, but Scola is proving that he will be a force in the pain for the Rockets this season. No Carl Landry and a limited Yao Ming means the uptick in production will continue for the Argentinian big man. Scola does not help out in blocks like other big men, but there is a real chance he could turn into a 20/10 guy with solid percentages this season ...
|h4||span style=color: #81c341;|Features: Purest Flavor, 2500mAh and 3.5ML Tank Capacity|/span||/h4| The VaporFi Rocket 3 provides a combination of innovation and quality found rarely in sub-ohm vape pens. The |a href=javascript: void(0) class=pop-u
Steve was first introduced to this incredible teaching tool in 2001 while speaking at the U.S. Space Camp for Educators. As part of the week-long experience, teachers learned how to use Homer Hickams Rocket Boys with multi-grade levels and in a variety of subject areas to not only inspire students to work hard to see that their dreams come true, but to also introduce and reinforce skills and concepts in some very creative ways. The documents below are offered by the U.S. Space Camp and the Space Academy for Educators in an effort to bring the wonder, discovery, and exploration of space and rocketry into a wide variety of classrooms.. ...
A while back my friend asked an easy way to make a bottle rocket .....so this Instructable was bornMake sure to wear the proper protective gear such as some kind of...
A Chinese Long March 2C rocket failed to reach the proper orbit after launching Thursday, likely destroying China's experimental SJ-11-04 satellite, according to state media.
PHOENIX (AP) -- Chris Paul scored 25 points to lead six Houston players in the double figures and the Rockets, still without injured James Harden, rolled past the Phoenix Suns 112-95 on Friday night.
The large hybrid rocket engine designed to power Britain’s supersonic Bloodhound car to supersonic speeds has undergone the first series of tests.
In an effort to prove the Earth is flat, 61-year-old "Mad" Mike Hughes plans to launch himself thousands of feet in the air in a homemade rocket. But his launch-which was originally set for Saturday-has been postponed, The Washington Post reported Friday afternoon.. .repubhubembed{display:none;}. ...
The fascinating story behind Britains Black Knight rocket and the top secret testing station established on The Needles headland in 1950s & 60s.
Main Components *Radio Controlled Receiver *4017 decade counter *Estes rocket igniters *A diode & transistor per active 4017 output pin. *One resistor...
Aerie recently reported the results of its second phase III registration trial in the United States named Rocket 2, where the primary efficacy endpoint was to demonstrate non-inferiority of IOP lowering for Rhopressa compared with timolol.
Reading Rockets offers information and resources on how children can learn to read and why many struggle and how caring adults can help
Join Dr. Greg Vogt as he discusses the history of rockets, and explains how astronauts and scientific experiments are launched into space.
The bully is firing snot rockets. He holds his head back and blocks one nostril with his finger, blowing out the other one. Small hard gobs of mucus are flying around him ...
TriSept Corp., a launch integration and mission management company based in Chantilly, Virginia, announced work Aug. 5 with Rocket Lab and Millennium Space Systems on Dragracer, a mission to demonstrate a way to de-orbit satellites at the conclusion of their missions.
The hits keep coming for the Los Angeles Lakers, who not only lost on Saturday after leading throughout regulation of a 138-134 loss to the Rockets in Houston, but also had to watch point guard
Weve been watching as the SpaceX Grasshoppers leap has grown higher and higher with each successive launch (and landing!), and the rockets flights never fa...
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Definition : Tubes covered with supporting media that are used to separate an ionic mixture of solutes into its components by the differences in migration rate when an electric field is applied (electrophoresis). These columns are usually made of glass or plastic (e.g., acrylic) tubes; they are usually mounted vertically, with both ends placed into buffer wells that make the electrical connection between the columns and the electrodes. Typically, several columns are run simultaneously in an electrophoretic system.. Entry Terms : "Columns, Electrophoresis" , "Immunoelectrophoresis Equipment" , "Apparatus, Electrophoresis" , "Electrophoresis Equipment" , "Chromatography Equipment". UMDC code : 18551 ...
Six chicken 7S immunoglobulin (Ig) allotypic specificities were demonstrated with antisera produced in highly inbred chickens. A radioimmunoassay which utilized intact, radiolabeled 7S Ig alloantigens was developed, and was used for the structural and genetic analysis of these specificities. The binding of dinitrophenylated alloantiserum (DAA) with radiolabeled alloantigen was demonstrated by the addition of rabbit anti-dinitrophenyl antiserum followed by precipitation of complexes with sheep anti-rabbit γ-globulin antiserum. Binding inhibition assays with the DAA method detected allotypes on 2 to 5 ng of 7S Ig alloantigen. Structural analyses of the six allotypic specificities were performed by direct binding and binding inhibition analysis with the DAA assay. Specificities CS-1.1 and CS-1.2 were present on papain-produced Fab fragments and isolated 7S Ig heavy (H) chains; but not on light (L) chains, Fc fragments, or 17S Ig. For both specificities H chains reassociated with L chains were ...
BROOMFIELD - Interns from a local aerospace company set the bar high Saturday for rocket launches at airshows everywhere - about 5,000 feet high.. The rocket launch that kicked off the Rocky Mountain Airshows second day was the first of its kind at an airshow. And getting Federal Aviation Administration officials to approve the launch has been about as difficult as rocket science. "Weve been working the details with them for over a year now to get this approved," said Greg Arend, of United Launch Alliance. Arend is structures development manager and intern rocket project manager for the Denver-based company. "They have regulations for air shows, and they have regulations for rocket launches. But they didnt - up until now - have regulations for rocket launches at airshows.". ULA interns spent their summer evenings building Stars N Stripes, the 17-foot rocket that accelerated to 300 mph in five seconds.. The high-powered rocket quickly disappeared into the atmosphere after taking off in a ...
Looking for online definition of Double Immunodiffusion in the Medical Dictionary? Double Immunodiffusion explanation free. What is Double Immunodiffusion? Meaning of Double Immunodiffusion medical term. What does Double Immunodiffusion mean?
Looking for online definition of alpha heavy chain disease in the Medical Dictionary? alpha heavy chain disease explanation free. What is alpha heavy chain disease? Meaning of alpha heavy chain disease medical term. What does alpha heavy chain disease mean?
1. 1. The presence of a low molecular weight protein (1.2 S), having antigenic determinants in common with the homologous Bence Jones protein (3.4 S), has been observed in the urine of a patient with multiple myeloma. Its amino acid composition and α-NH2-terminal amino acid residue make it likely that ... read more the 1.2 S component is a protein representing the NH2-terminal, variable, moiety of the Bence Jones protein. 2. 2. After digesting Bence Jones protein (type K, InV(a−)) with pepsin, an antigenically active peptide could be isolated via immunoprecipitation with the homologous antiserum. From its amino acid composition this peptide appears to be located at the COOH-terminal end of the Bence Jones protein, i.e. in the constant part of the polypeptide chain; it must perforce contain the group-specific antigenic determinant. show less ...
HOUSTON - The wait is almost over. In little more than 24 hours, the Rockets will tip-off the preseason portion of their 2012-13 schedule when they take on Kevin Durant, Russell Westbrook and the rest of the reigning Western Conference champions from Oklahoma City Wednesday night in McAllen, Texas.. And while the star power on hand certainly adds to the anticipation of and build up for tomorrows exhibition opener, lets face it, from a Rockets perspective the opponent is largely irrelevant. Houstons coaching staff wont be worried about what KD and company do; theyll simply be concerned with watching to see whether or not their own players can execute the game plan and play within the system. Or as assistant coach Kelvin Sampson told his club during Mondays practice: "Basketball isnt a game of great plays; its a game of eliminating mistakes." All eyes, then, should be focused on how cleanly Houstons young players can function on the floor together, and the amount of error-free ball they ...
Abstract Echography and/or chest radiograph in association with six serologic (immunologic) methods were tested for their ability to diagnose human hydatidosis. The immunologic techniques used were latex agglutination, counter immunoelectrophoresis, enzyme-linked immunosorbent assay (ELISA) with whole hydatid fluid and with antigen 5, a thermobabile lipoprotein that elicits the arc 5 precipitin line in immunoelectrophoresis. In this report, the results of examinations of 273 patients are presented. Of these, 243 cases were surgically proven to have hydatidosis, whereas 30 of the 273 that were strongly suspected of having hydatid cysts by radiology were shown to have other pathologies. The ELISA was more sensitive than the other methods, with 204 of 243 sera shown to be positive. The remaining 39 sera that gave false-negative results were tested using two recently developed methods, enzyme-linked immunoelectrodiffusion assay (ELIEDA) and immunoblotting (electrophoretic migration of hydatid fluid antigens
Objective. To describe the effect of HIV serostatus on serum proteins, serum protein electrophoresis (SPEP) patterns and monoclonal bands. Setting. Inkosi Albert Luthuli Central Hospital, Durban. Design. Retrospective, anonymous analysis of routine laboratory results. Results. Monoclonal bands were not increased in HIV-positive patients, who were younger and had increased polyclonal and oligoclonal bands and total proteins when compared with HIV-negative patients.
  • Immunoelectrophoresis differs from blotting techniques because with IE the entire procedure is conducted in an agarose gel and blotting is not necessary. (els.net)
  • Crossed immunoelectrophoresis has been used for studies of proteins in biological fluids, particularly human serum, and biological extracts. (wikipedia.org)
  • Affinity immunoelectrophoresis is based on changes in the electrophoretic pattern of proteins through specific interaction or complex formation with other macromolecules or ligands. (wikipedia.org)
  • Affinity immunoelectrophoresis has been used for estimation of binding constants, as for instance with lectins or for characterization of proteins with specific features like glycan content or ligand binding. (wikipedia.org)
  • Immunoelectrophoresis is an older method for qualitative analysis of M-proteins in serum and urine. (microbenotes.com)
  • This rule applies only to the specimen and would appear not to allow ordering of an Immunoelectrophoresis on an additional blood specimen. (thefreedictionary.com)
  • Immunoelectrophoresis created a breakthrough in protein identification and in immunology. (microbenotes.com)
  • On the basis of these findings, immunoelectrophoresis is considered to be a reliable method for differentiation between tumour tissue of neuroectodermal and non-neuroectodermal origin. (springer.com)