Technique involving the diffusion of antigen or antibody through a semisolid medium, usually agar or agarose gel, with the result being a precipitin reaction.
A technique that combines protein electrophoresis and double immunodiffusion. In this procedure proteins are first separated by gel electrophoresis (usually agarose), then made visible by immunodiffusion of specific antibodies. A distinct elliptical precipitin arc results for each protein detectable by the antisera.
Antibodies which elicit IMMUNOPRECIPITATION when combined with antigen.
Serum that contains antibodies. It is obtained from an animal that has been immunized either by ANTIGEN injection or infection with microorganisms containing the antigen.
Serologic tests based on inactivation of complement by the antigen-antibody complex (stage 1). Binding of free complement can be visualized by addition of a second antigen-antibody system such as red cells and appropriate red cell antibody (hemolysin) requiring complement for its completion (stage 2). Failure of the red cells to lyse indicates that a specific antigen-antibody reaction has taken place in stage 1. If red cells lyse, free complement is present indicating no antigen-antibody reaction occurred in stage 1.
Immunoglobulins produced in a response to FUNGAL ANTIGENS.
Serological reactions in which an antiserum against one antigen reacts with a non-identical but closely related antigen.
Chemical analysis based on the phenomenon whereby light, passing through a medium with dispersed particles of a different refractive index from that of the medium, is attenuated in intensity by scattering. In turbidimetry, the intensity of light transmitted through the medium, the unscattered light, is measured. In nephelometry, the intensity of the scattered light is measured, usually, but not necessarily, at right angles to the incident light beam.
Substances of fungal origin that have antigenic activity.
A mitosporic fungal genus which causes COCCIDIOIDOMYCOSIS.
Sensitive tests to measure certain antigens, antibodies, or viruses, using their ability to agglutinate certain erythrocytes. (From Stedman, 26th ed)
Infection with a fungus of the genus COCCIDIOIDES, endemic to the SOUTHWESTERN UNITED STATES. It is sometimes called valley fever but should not be confused with RIFT VALLEY FEVER. Infection is caused by inhalation of airborne, fungal particles known as arthroconidia, a form of FUNGAL SPORES. A primary form is an acute, benign, self-limited respiratory infection. A secondary form is a virulent, severe, chronic, progressive granulomatous disease with systemic involvement. It can be detected by use of COCCIDIOIDIN.
Substances that are recognized by the immune system and induce an immune reaction.
Viral disease of horses caused by the equine infectious anemia virus (EIAV; INFECTIOUS ANEMIA VIRUS, EQUINE). It is characterized by intermittent fever, weakness, and anemia. Chronic infection consists of acute episodes with remissions.
Substances elaborated by bacteria that have antigenic activity.
The species Oryctolagus cuniculus, in the family Leporidae, order LAGOMORPHA. Rabbits are born in burrows, furless, and with eyes and ears closed. In contrast with HARES, rabbits have 22 chromosome pairs.
The type species of DELTARETROVIRUS that causes a form of bovine lymphosarcoma (ENZOOTIC BOVINE LEUKOSIS) or persistent lymphocytosis.
The major immunoglobulin isotype class in normal human serum. There are several isotype subclasses of IgG, for example, IgG1, IgG2A, and IgG2B.
A series of steps taken in order to conduct research.
A lymphoid neoplastic disease in cattle caused by the bovine leukemia virus. Enzootic bovine leukosis may take the form of lymphosarcoma, malignant lymphoma, or leukemia but the presence of malignant cells in the blood is not a consistent finding.
Chronic respiratory disease caused by the VISNA-MAEDI VIRUS. It was formerly believed to be identical with jaagsiekte (PULMONARY ADENOMATOSIS, OVINE) but is now recognized as a separate entity.
Sites on an antigen that interact with specific antibodies.
Discontinuation of the habit of smoking, the inhaling and exhaling of tobacco smoke.
The sum of the weight of all the atoms in a molecule.
A mitosporic fungal genus. P. brasiliensis (previously Blastomyces brasiliensis) is the etiologic agent of PARACOCCIDIOIDOMYCOSIS.
A mitosporic Onygenales fungal genus causing HISTOPLASMOSIS in humans and animals. Its single species is Histoplasma capsulatum which has two varieties: H. capsulatum var. capsulatum and H. capsulatum var. duboisii. Its teleomorph is AJELLOMYCES capsulatus.
Studies determining the effectiveness or value of processes, personnel, and equipment, or the material on conducting such studies. For drugs and devices, CLINICAL TRIALS AS TOPIC; DRUG EVALUATION; and DRUG EVALUATION, PRECLINICAL are available.
An immunoassay utilizing an antibody labeled with an enzyme marker such as horseradish peroxidase. While either the enzyme or the antibody is bound to an immunosorbent substrate, they both retain their biologic activity; the change in enzyme activity as a result of the enzyme-antibody-antigen reaction is proportional to the concentration of the antigen and can be measured spectrophotometrically or with the naked eye. Many variations of the method have been developed.
A genus of destructive root-parasitic OOMYCETES in the family Pythiaceae, order Peronosporales, commonly found in cultivated soils all over the world. Differentiation of zoospores takes place in a vesicle.
Infection resulting from inhalation or ingestion of spores of the fungus of the genus HISTOPLASMA, species H. capsulatum. It is worldwide in distribution and particularly common in the midwestern United States. (From Dorland, 27th ed)
Electrophoresis in which discontinuities in both the voltage and pH gradients are introduced by using buffers of different composition and pH in the different parts of the gel column. The term 'disc' was originally used as an abbreviation for 'discontinuous' referring to the buffers employed, and does not have anything to do with the shape of the separated zones.
Electrophoresis in which a polyacrylamide gel is used as the diffusion medium.
Diagnostic procedures involving immunoglobulin reactions.
Any of numerous agile, hollow-horned RUMINANTS of the genus Capra, in the family Bovidae, closely related to the SHEEP.
Chromatography on non-ionic gels without regard to the mechanism of solute discrimination.
Immunoelectrophoresis in which immunoprecipitation occurs when antigen at the cathode is caused to migrate in an electric field through a suitable medium of diffusion against a stream of antibody migrating from the anode as a result of endosmotic flow.
Proteins that regulate the signaling activity of GTP-BINDING PROTEINS. They are divided into three categories depending upon whether they stimulate GTPase activity (GTPASE-ACTIVATING PROTEINS), inhibit release of GDP; (GUANINE NUCLEOTIDE DISSOCIATION INHIBITORS); or exchange GTP for GDP; (GUANINE NUCLEOTIDE EXCHANGE FACTORS).
A species of LENTIVIRUS, subgenus equine lentiviruses (LENTIVIRUSES, EQUINE), causing acute and chronic infection in horses. It is transmitted mechanically by biting flies, mosquitoes, and midges, and iatrogenically through unsterilized equipment. Chronic infection often consists of acute episodes with remissions.
Classic quantitative assay for detection of antigen-antibody reactions using a radioactively labeled substance (radioligand) either directly or indirectly to measure the binding of the unlabeled substance to a specific antibody or other receptor system. Non-immunogenic substances (e.g., haptens) can be measured if coupled to larger carrier proteins (e.g., bovine gamma-globulin or human serum albumin) capable of inducing antibody formation.
Organic compounds that generally contain an amino (-NH2) and a carboxyl (-COOH) group. Twenty alpha-amino acids are the subunits which are polymerized to form proteins.
Field of chemistry that pertains to immunological phenomena and the study of chemical reactions related to antigen stimulation of tissues. It includes physicochemical interactions between antigens and antibodies.
Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
Large, hoofed mammals of the family EQUIDAE. Horses are active day and night with most of the day spent seeking and consuming food. Feeding peaks occur in the early morning and late afternoon, and there are several daily periods of rest.
Diseases of domestic cattle of the genus Bos. It includes diseases of cows, yaks, and zebus.
Immunoelectrophoresis in which a second electrophoretic transport is performed on the initially separated antigen fragments into an antibody-containing medium in a direction perpendicular to the first electrophoresis.
A computer based method of simulating or analyzing the behavior of structures or components.
Process of determining and distinguishing species of bacteria or viruses based on antigens they share.
Immunoglobulins produced in response to VIRAL ANTIGENS.
Tests that are dependent on the clumping of cells, microorganisms, or particles when mixed with specific antiserum. (From Stedman, 26th ed)
Domesticated bovine animals of the genus Bos, usually kept on a farm or ranch and used for the production of meat or dairy products or for heavy labor.
Serologic tests in which a positive reaction manifested by visible CHEMICAL PRECIPITATION occurs when a soluble ANTIGEN reacts with its precipitins, i.e., ANTIBODIES that can form a precipitate.
Glycoproteins with the electrophoretic mobility of BETA-GLOBULINS, secreted by the placental TROPHOBLASTS into the maternal bloodstream during PREGNANCY. They can be detected 18 days after OVULATION and reach 200 mg/ml at the end of pregnancy. They are associated with fetal well-being.
A genus of fungi in the family Entomophthoraceae, order Entomorphthorales. They are primarily parasites of insects and spiders, but have been found to cause mycotic infections of the nose in man and horses.
Insertion of viral DNA into host-cell DNA. This includes integration of phage DNA into bacterial DNA; (LYSOGENY); to form a PROPHAGE or integration of retroviral DNA into cellular DNA to form a PROVIRUS.
A class of immunoglobulin bearing mu chains (IMMUNOGLOBULIN MU-CHAINS). IgM can fix COMPLEMENT. The name comes from its high molecular weight and originally being called a macroglobulin.
A mycosis affecting the skin, mucous membranes, lymph nodes, and internal organs. It is caused by Paracoccidioides brasiliensis. It is also called paracoccidioidal granuloma. Superficial resemblance of P. brasiliensis to Blastomyces brasiliensis (BLASTOMYCES) may cause misdiagnosis.
Represents 15-20% of the human serum immunoglobulins, mostly as the 4-chain polymer in humans or dimer in other mammals. Secretory IgA (IMMUNOGLOBULIN A, SECRETORY) is the main immunoglobulin in secretions.
A technique using antibodies for identifying or quantifying a substance. Usually the substance being studied serves as antigen both in antibody production and in measurement of antibody by the test substance.
The restriction of a characteristic behavior, anatomical structure or physical system, such as immune response; metabolic response, or gene or gene variant to the members of one species. It refers to that property which differentiates one species from another but it is also used for phylogenetic levels higher or lower than the species.
Multi-subunit proteins which function in IMMUNITY. They are produced by B LYMPHOCYTES from the IMMUNOGLOBULIN GENES. They are comprised of two heavy (IMMUNOGLOBULIN HEAVY CHAINS) and two light chains (IMMUNOGLOBULIN LIGHT CHAINS) with additional ancillary polypeptide chains depending on their isoforms. The variety of isoforms include monomeric or polymeric forms, and transmembrane forms (B-CELL ANTIGEN RECEPTORS) or secreted forms (ANTIBODIES). They are divided by the amino acid sequence of their heavy chains into five classes (IMMUNOGLOBULIN A; IMMUNOGLOBULIN D; IMMUNOGLOBULIN E; IMMUNOGLOBULIN G; IMMUNOGLOBULIN M) and various subclasses.
A form of alveolitis or pneumonitis due to an acquired hypersensitivity to inhaled antigens associated with farm environment. Antigens in the farm dust are commonly from bacteria actinomycetes (SACCHAROPOLYSPORA and THERMOACTINOMYCES), fungi, and animal proteins in the soil, straw, crops, pelts, serum, and excreta.
Electrophoresis in which a pH gradient is established in a gel medium and proteins migrate until they reach the site (or focus) at which the pH is equal to their isoelectric point.
The largest class of organic compounds, including STARCH; GLYCOGEN; CELLULOSE; POLYSACCHARIDES; and simple MONOSACCHARIDES. Carbohydrates are composed of carbon, hydrogen, and oxygen in a ratio of Cn(H2O)n.
All blood proteins except albumin ( = SERUM ALBUMIN, which is not a globulin) and FIBRINOGEN (which is not in the serum). The serum globulins are subdivided into ALPHA-GLOBULINS; BETA-GLOBULINS; and GAMMA-GLOBULINS on the basis of their electrophoretic mobilities. (From Dorland, 28th ed)
Members of the phylum Arthropoda, composed of organisms having a hard, jointed exoskeleton and paired jointed legs. It includes the class INSECTS and the subclass ARACHNIDA, many species of which are important medically as parasites or as vectors of organisms capable of causing disease in man.
Immunoglobulin molecules having a specific amino acid sequence by virtue of which they interact only with the ANTIGEN (or a very similar shape) that induced their synthesis in cells of the lymphoid series (especially PLASMA CELLS).
The type species of YATAPOXVIRUS, a tumor-producing DNA virus discovered in monkeys in Yaba, Nigeria. It has been found to produce histiocytomas in monkeys and humans.
Serum globulins that migrate to the gamma region (most positively charged) upon ELECTROPHORESIS. At one time, gamma-globulins came to be used as a synonym for immunoglobulins since most immunoglobulins are gamma globulins and conversely most gamma globulins are immunoglobulins. But since some immunoglobulins exhibit an alpha or beta electrophoretic mobility, that usage is in decline.
Diseases of the domestic or wild goat of the genus Capra.
A genus of onygenacetous mitosporic fungi whose perfect state is Ajellomyces (see ONYGENALES). The species Blastomyces dermatitidis (perfect state Ajellomyces dermatitidis) causes blastomycosis.
Antigens of the virion of the HEPATITIS B VIRUS or the Dane particle, its surface (HEPATITIS B SURFACE ANTIGENS), core (HEPATITIS B CORE ANTIGENS), and other associated antigens, including the HEPATITIS B E ANTIGENS.
A species of LENTIVIRUS, subgenus ovine-caprine lentiviruses (LENTIVIRUSES, OVINE-CAPRINE), that can cause chronic pneumonia (maedi), mastitis, arthritis, and encephalomyelitis (visna) in sheep. Maedi is a progressive pneumonia of sheep which is similar to but not the same as jaagsiekte (PULMONARY ADENOMATOSIS, OVINE). Visna is a demyelinating leukoencephalomyelitis of sheep which is similar to but not the same as SCRAPIE.
A genus of PASTEURELLACEAE described as gram-negative, nonsporeforming, nonmotile, facultative anaerobes. Most members are found both as pathogens and commensal organisms in the respiratory, alimentary, and genital tracts of animals.
The formation of a solid in a solution as a result of a chemical reaction or the aggregation of soluble substances into complexes large enough to fall out of solution.
The pH in solutions of proteins and related compounds at which the dipolar ions are at a maximum.
Complex neurobehavioral disorder characterized by distinctive facial features (FACIES), developmental delay and INTELLECTUAL DISABILITY. Behavioral phenotypes include sleep disturbance, maladaptive, self-injurious and attention-seeking behaviors. The sleep disturbance is linked to an abnormal circadian secretion pattern of MELATONIN. The syndrome is associated with de novo deletion or mutation and HAPLOINSUFFICIENCY of the retinoic acid-induced 1 protein on chromosome 17p11.2.
The protein components that constitute the common core of small nuclear ribonucleoprotein particles. These proteins are commonly referred as Sm nuclear antigens due to their antigenic nature.
Serologic tests in which a known quantity of antigen is added to the serum prior to the addition of a red cell suspension. Reaction result is expressed as the smallest amount of antigen which causes complete inhibition of hemagglutination.
Techniques used to separate mixtures of substances based on differences in the relative affinities of the substances for mobile and stationary phases. A mobile phase (fluid or gas) passes through a column containing a stationary phase of porous solid or liquid coated on a solid support. Usage is both analytical for small amounts and preparative for bulk amounts.
Any of the ruminant mammals with curved horns in the genus Ovis, family Bovidae. They possess lachrymal grooves and interdigital glands, which are absent in GOATS.
Virus diseases caused by the Lentivirus genus. They are multi-organ diseases characterized by long incubation periods and persistent infection.
Broad spectrum antifungal agent used for long periods at high doses, especially in immunosuppressed patients.
Techniques used to demonstrate or measure an immune response, and to identify or measure antigens using antibodies.
Polysaccharides found in bacteria and in capsules thereof.
A synthetic retinoid that is used orally as a chemopreventive against prostate cancer and in women at risk of developing contralateral breast cancer. It is also effective as an antineoplastic agent.
Antisera from immunized animals that is purified and used as a passive immunizing agent against specific BACTERIAL TOXINS.
The measurement of infection-blocking titer of ANTISERA by testing a series of dilutions for a given virus-antiserum interaction end-point, which is generally the dilution at which tissue cultures inoculated with the serum-virus mixtures demonstrate cytopathology (CPE) or the dilution at which 50% of test animals injected with serum-virus mixtures show infectivity (ID50) or die (LD50).
A family of sterols commonly found in plants and plant oils. Alpha-, beta-, and gamma-isomers have been characterized.
The thin, yellow, serous fluid secreted by the mammary glands during pregnancy and immediately postpartum before lactation begins. It consists of immunologically active substances, white blood cells, water, protein, fat, and carbohydrates.
An electrochemical process in which macromolecules or colloidal particles with a net electric charge migrate in a solution under the influence of an electric current.
A genus of gram-positive, coccoid bacteria whose organisms occur in pairs or chains. No endospores are produced. Many species exist as commensals or parasites on man or animals with some being highly pathogenic. A few species are saprophytes and occur in the natural environment.
Commercially prepared reagent sets, with accessory devices, containing all of the major components and literature necessary to perform one or more designated diagnostic tests or procedures. They may be for laboratory or personal use.
A species of the genus BRUCELLA whose natural hosts are cattle and other bovidae. Abortion and placentitis are frequently produced in the pregnant animal. Other mammals, including humans, may be infected.
Viruses whose taxonomic relationships have not been established.
A glycoprotein that is central in both the classical and the alternative pathway of COMPLEMENT ACTIVATION. C3 can be cleaved into COMPLEMENT C3A and COMPLEMENT C3B, spontaneously at low level or by C3 CONVERTASE at high level. The smaller fragment C3a is an ANAPHYLATOXIN and mediator of local inflammatory process. The larger fragment C3b binds with C3 convertase to form C5 convertase.
A 53-kDa protein that is a positive regulator of the alternate pathway of complement activation (COMPLEMENT ACTIVATION PATHWAY, ALTERNATIVE). It stabilizes the ALTERNATIVE PATHWAY C3 CONVERTASE (C3bBb) and protects it from rapid inactivation, thus facilitating the cascade of COMPLEMENT ACTIVATION and the formation of MEMBRANE ATTACK COMPLEX. Individuals with mutation in the PFC gene exhibit properdin deficiency and have a high susceptibility to infections.
Centrifugation with a centrifuge that develops centrifugal fields of more than 100,000 times gravity. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)
A chromatographic technique that utilizes the ability of biological molecules to bind to certain ligands specifically and reversibly. It is used in protein biochemistry. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)
Autoantibodies directed against various nuclear antigens including DNA, RNA, histones, acidic nuclear proteins, or complexes of these molecular elements. Antinuclear antibodies are found in systemic autoimmune diseases including systemic lupus erythematosus, Sjogren's syndrome, scleroderma, polymyositis, and mixed connective tissue disease.
Substances that are toxic to the intestinal tract causing vomiting, diarrhea, etc.; most common enterotoxins are produced by bacteria.
A collection of single-stranded RNA viruses scattered across the Bunyaviridae, Flaviviridae, and Togaviridae families whose common property is the ability to induce encephalitic conditions in infected hosts.
An organism of the vegetable kingdom suitable by nature for use as a food, especially by human beings. Not all parts of any given plant are edible but all parts of edible plants have been known to figure as raw or cooked food: leaves, roots, tubers, stems, seeds, buds, fruits, and flowers. The most commonly edible parts of plants are FRUIT, usually sweet, fleshy, and succulent. Most edible plants are commonly cultivated for their nutritional value and are referred to as VEGETABLES.
The type species of ORBIVIRUS causing a serious disease in sheep, especially lambs. It may also infect wild ruminants and other domestic animals.
The production of ANTIBODIES by proliferating and differentiated B-LYMPHOCYTES under stimulation by ANTIGENS.
Proteins that are present in blood serum, including SERUM ALBUMIN; BLOOD COAGULATION FACTORS; and many other types of proteins.
The study of serum, especially of antigen-antibody reactions in vitro.
A species of LENTIVIRUS, subgenus ovine-caprine lentiviruses (LENTIVIRUSES, OVINE-CAPRINE), closely related to VISNA-MAEDI VIRUS and causing acute encephalomyelitis; chronic arthritis; PNEUMONIA; MASTITIS; and GLOMERULONEPHRITIS in goats. It is transmitted mainly in the colostrum and milk.
Any of various animals that constitute the family Suidae and comprise stout-bodied, short-legged omnivorous mammals with thick skin, usually covered with coarse bristles, a rather long mobile snout, and small tail. Included are the genera Babyrousa, Phacochoerus (wart hogs), and Sus, the latter containing the domestic pig (see SUS SCROFA).

The isolation and partial characterization of the serum lipoproteins and apolipoproteins of the rainbow trout. (1/3737)

1. VLD (very-low-density), LD (low-density) and HD (high-density) lipoproteins were isolated from the serum of trout (Salmo gairdneri Richardson). 2. Each lipoprotein class resembled that of the human in immunological reactivity, electrophoretic behaviour and appearance in the electron microscope. Trout LD lipoprotein, however, was of greater density than human LD lipoprotein. 3. The trout lipoproteins have lipid compositions which are similar to those of the corresponding human components, except for their high contents of long-chain unsaturated fatty acids. 4. HD and LD lipoproteins were immunologically non-identical, whereas LD lipoproteins possessed antigenic determinants in common with VLD lipoproteins. 5. VLD and HD lipoproteins each contained at least seven different apoproteins, whereas LD liprotein was composed largely of a single apoprotein which resembled human apolipoprotein B. 6. At least one, and possibly three, apoprotein of trout HD lipoprotein showed features which resemble human apoprotein A-1.7. The broad similarity between the trout and human lipoprotein systems suggests that both arose from common ancestral genes early in evolutionary history.  (+info)

Studies of the binding of different iron donors to human serum transferrin and isolation of iron-binding fragments from the N- and C-terminal regions of the protein. (2/3737)

1. Trypsin digestion of human serum transferrin partially saturated with iron(III)-nitrilotriacetate at pH 5.5 or pH 8.5 produces a carbohydrate-containing iron-binding fragment of mol.wt. 43000. 2. When iron(III) citrate, FeCl3, iron (III) ascorabate and (NH4)2SO4,FeSO4 are used as iron donors to saturate the protein partially, at pH8.5, proteolytic digestion yields a fragment of mol.wt. 36000 that lacks carbohydrate. 3. The two fragments differ in their antigenic structures, amino acid compositions and peptide 'maps'. 4. The fragment with mol.wt. 36000 was assigned to the N-terminal region of the protein and the other to the C-terminal region. 5. The distribution of iron in human serum transferrin partially saturated with various iron donors was examined by electrophoresis in urea/polyacrylamide gels and the two possible monoferric forms were unequivocally identified. 6. The site designated A on human serum transferrin [Harris (1977) Biochemistry 16, 560--564] was assigned to the C-terminal region of the protein and the B site to the N-terminal region. 7. The distribution of iron on transferrin in human plasma was determined.  (+info)

Mushroom worker's lung resulting from indoor cultivation of Pleurotus osteatus. (3/3737)

Indoor cultivation of oyster mushroom Pleurotus osteatus lead to an outbreak of extrinsic allergic alveolitis in two workers. High titer of indirect fluorescent antibody and positive precipitins against basidiospores of P. osteatus were demonstrated in sera of the patients. Mushroom workers should protect themselves from the basidiospores, being aware of their pathogenicity.  (+info)

Chemical and immunochemical measurement of total iron-binding capacity compared. (4/3737)

Radiometric, colorimetric, and two immunochemical methods for measuring total iron-binding capacity are compared. We evaluated the procedures on the basis of precision, applicability to a pediatric population, and accuracy as assessed by analytical recovery of purified transferrin. The immunoephelometric assay for transferrin provides significant advantages over the other methods examined.  (+info)

Purification of two dexamethasone-binding proteins from rat-liver cytosol. (5/3737)

Two dexamethasone-binding proteins have been purified from rat liver cytosol. The main purification steps are: precipitation by protamine sulphate, affinity chromatography on CH-Sepharose 4B to which 11-deoxycorticosterone is linked through a disulfide bond and DEAE-cellulose chromatography. Two binding components elute from the DEAE-cellulose column at 0.12 M and 0.2 M NaCl, respectively. By means of dodecylsulphate/polyacrylamide gel electrophoresis it was demonstrated that both components are composed predominantly of a single polypeptide with molecular weights of about 45 000 and 90 000. Antibodies to the two polypeptides have been elicited in rabbits. The antibodies to the 45 000-Mr polypeptide cross react with the 90 000-Mr component. Likewise the antibodies to the 90 000-Mr protein precipitate the 45 000-Mr polypeptide. Either of the two antibody preparations immunoprecipitates the major part (approximately 70%) of the dexamethasone-binding activity of the cytosol.  (+info)

Carbohydrate on human factor VIII/von Willebrand factor. Impairment of function by removal of specific galactose residues. (6/3737)

Human factor VIII/von Willebrand factor protein containing 120 +/- 12 nmol of sialic acid and 135 +/- 13 nmol of galactose/mg of protein was digested with neuraminidase. The affinity of native factor VIII/von Willebrand factor and its asialo form for the hepatic lectin that specifically binds asialoglycoproteins was assessed from in vitro binding experiments. Native factor VIII/von Willebrand factor exhibited negligible affinity while binding of the asialo derivative was comparable to that observed for asialo-alpha1-acid glycoprotein. Incubation of asialo-factor VIII/von Willebrand factor with Streptococcus pneumoniae beta-galactosidase removed only 62% of the galactose but abolished binding to the purified hepatic lectin. When the asialo derivative was incubated with purified beta-D-galactoside alpha2 leads to 6 sialyltransferase and CMP-[14C]NeuAc, only 61% of the galactose incorporated [14C]NeuAc. From the known specificites of these enzymes, it is concluded that galactose residues important in lectin binding are present in a terminal Gal/beta1 leads to 4GlcNAc sequence on asialo-factor VIII/von Willebrand factor. The relative ristocetin-induced platelet aggregating activity of native, asialo-, and agalacto-factor VIII/von Willebrand factor was 100:38:12, respectively, while procoagulant activity was 100:100:103.  (+info)

Immunological comparison of the proteins of chicken and rat liver ribosomes. (7/3737)

A comparison of the proteins of chicken and rat liver ribosomes using immunochemical techniques was undertaken. The procedures included quantitative precipitation, passive hemagglutination, and immunodiffusion on Ouchterlony plates. The results indicate that antisera specific for chicken or rat liver ribosomes recognize only about 20% of common determinants. While there are important reservations, the results suggest extensive differences in the proteins of rat and chicken liver ribosomes. Despite those differences, rat and chicken liver ribosomal proteins maintain some homologous sequences present in bacterial ribosomal proteins. An enriched antibody preparation against chicken 80 S ribosomes inhibited the poly(U)-directed synthesis of polyphenylalanine and the elongation factor G (EF-G)-catalyzed binding of [3H]GDP to Escherichia coli ribosomes. Thus, chicken liver ribosomes, like ribosomes from rat liver and yeast, must have proteins homologous with those of E. coli ribosomes.  (+info)

Homogeneous pyruvate kinase isolated from yeast by two different methods is indistinguishable from pyruvate kinase in cell-free extract. (8/3737)

In this report, we have compared homogeneous yeast (Saccharomyces cerevisiae) pyruvate kinase to enzyme from cell-free extracts in several different ways: 1) isoelectric focusing of cell-free extracts indicates one peak of pyruvate kinase activity whose isoelectric point is the same as that of the pure enzyme; 2) antibody prepared to the pure enzyme produces a single, fused precipitin line against enzyme in the cell-free extract and pure enzyme; 3) immunoelectrophoresis of cell-free extract produces one precipitin arc which has the same mobility as that of the pure enzyme; and 4) immunoprecipitation of the pure enzyme from cell-free extract with subsequent solubilization in 1% sodium dodecyl sulfate and electrophoresis on sodium dodecyl sulfate-polyacrylamide gels produces a single protein band attributable to pyruvate kinase which co-migrates with the purified enzyme. Within the limits of the sensitivity of the methods employed, we conclude that the homogeneous pyruvate kinase prepared from yeast lysed either by Manton-Gaulin homogenization (Aust, A., Yun, S.-L., and Suelter, C. (1975) Methods Enzymol. 42, 176-182) or by toluolysis (Yun, S.-L., Aust, A.E., and Suelter, C.H. (1977) J. Biol. Chem. 251, 124-128) is identical with pyruvate kinase in cell-free extract.  (+info)

Silver-staining of immunoprecipitates extends the sensitivity of the radial immunodiffusion assay by tenfold. This modification permits the quantification of apolipoproteins A-I, A-II, C, and E at levels of 0.2-1.0 mg/dl in plasma samples at a sensitivity threshold of 10 ng. The silver-enhanced radial immunodiffusion method is readily adapted from the standard method, simple and inexpensive to perform, and does not require costly instrumentation. These advantages make the modified RID assay an attractive alternative to other forms of immunoassay.
This lab activity is designed to study highly specific lock-key matching properties of antigen-antibody and how this highly specific interaction can be exploited as a tool for research and analysis. This study involves the use of an immunodiffusion technique in which antigen and antibody are allowed to diffuse in a solid agarose medium. When antigen and antibody meet, antigen-antibody complex is formed, which leads to precipitation. Antigen-antibody precipitate is formed in the zone where the concentration of the two matching pair reaches an optimal known as the zone of equivalence, which results in formation of a visible opaque precipitate region in agarose medium. Those regions of precipitation can be used for determination of concentration or titer of both antigen and antibody. The Antigen-Antibody Interaction kit is a hands-on study of both Ouchterlony Double Diffusion and Radial Immunodiffusion techniques. This kit also provides additional guidance materials for teaching other types of ...
Tumor antigens on the cell surface of thymic lymphoma cells from chemical- and virus-induced lymphomas of C57BL mice and virus-induced tumors of Wistar-Furth rats have been studied with the use of immunofluorescence on viable cells with rat, rabbit, and monkey antisera. In young mice given intrathymic injections of a murine leukemia virus, a new cell surface antigen can be detected as early as 2 to 4 days postinjection in some thymuses. Rat antisera to virus-induced rat thymic lymphoma cells gave a precipitin line in Ouchterlony double diffusion analysis when tested with virus isolated from plasma of tumor-bearing rats or from mouse lymphoma extracts. This reaction is due to the group-specific (internal) antigen of the murine leukemia viruses, since ether treatment of the virus preparations was required to obtain it. The results indicate that both radiation and certain chemicals may activate the same leukemia virus, which is endemic in this low leukemia strain.. ...
We investigated the effect of fibronectin on epithelial migration onto the stroma in cultured rabbit cornea. Rabbit plasma fibronectin was purified by affinity chromatography using gelatin-Sepharose 4B, and its purity was confirmed by SDS polyacrylamide slab gel electrophoresis. Antibody against rabbit plasma fibronectin raised in guinea pigs formed a single precipitin line against rabbit plasma and purified rabbit plasma fibronectin by Ouchterlony double diffusion test. When rabbit cornea was cut into small blocks and cultured in TCM-199 medium alone, corneal epithelial cells began to migrate on the cut edge of the corneal stroma. The addition of purified rabbit plasma fibronectin to the culture medium significantly enhanced epithelial migration. The degree of enhancement depended on the amount of fibronectin added. When guinea pig IgG anti-rabbit plasma fibronectin was added, epithelial migration was significantly inhibited when compared with that in control cultured corneal blocks. The ...
Definition of double diffusion precipitin test in the Financial Dictionary - by Free online English dictionary and encyclopedia. What is double diffusion precipitin test? Meaning of double diffusion precipitin test as a finance term. What does double diffusion precipitin test mean in finance?
The defined antibody specificity is directed to albumin as tested against cat sera. In immunoelectrophoresis and double radial immunodiffusion (Ouchterlony), using various antiserum concentrations against appropriate concentrations of the immunogen, a single characteristic precipitin line is obtained which shows a reaction of identity with the precipitin lines obtained against cat serum and the purified albumin. As precipitating antiserum to identify or measure cat albumin by a variety of immunodiffusion techniques, including immunoelectrophoresis, single and double radial immunodiffusion (Mancini, Ouchterlony) and electroimmunodiffusion (Laurell). It has not been tested for use in nephelometry, ELISA or immunochemistry, but this does not exclude such use if proper controls are included ...
As a qualitative serum with an optimized protein content to study the normal protein composition of bovine serum. To identify or quantitate a normal serum protein component using a variety of immunodiffusion techniques including immunoelectrophoresis, single and double radial immunodiffusion and electroimmunodiffusion. As a reference serum in nephelometry and other automated precipitation techniques. As a blocking agent or as a negative control in non-precipitating antibody-binding assays e.g. in serodiagnostic immunofluorescence and immunoenzyme tests. This normal reference serum does not contain assigned values for individual proteins but can be used as an internal relative standard for quantitative protein assays such as double radial immunodiffusion (Mancini, Fahey), ELISA, Western immunoblotting and electroimmunodiffusion (Laurell), expressing the results obtained with the serum sample under investigation as a percentage of the protein concentration in the normal reference serum ...
Looking for immunodiffusion? Find out information about immunodiffusion. A serological procedure in which antigen and antibody solutions are permitted to diffuse toward each other through a gel matrix; interaction is manifested... Explanation of immunodiffusion
Immunodiffusion Plates are polystyrene with close-fitting covers to minimize gel dehydration. Plate bottoms are recessed 7 mm to prevent scratching on the bench surfaces. The plate is hydrophilic for easy gel pouring and better adherence to the plate.
Hyperactive secretion and pathogenic effects of interleukin (IL)-17 and IgA have been detected in different arthropathies. Recent evidence has revealed that TH17 cytokines regulate mucosal IgA secretion. However, it is unknown whether and how IL-17 mediates synovial IgA production. Here we aim to investigate the connection of synovial IL-17 with IgA production in the joint. In this study we included synovial fluids (SF) from patients with rheumatoid arthritis (RA; n = 66), spondyloarthritis (SpA; n = 18) and osteoarthritis (OA; n = 36). The levels of IL-17, IL-6, transforming growth factor (TGF)-β1, B-cell-activating factor of the TNF family (BAFF) and anti-lipopolyssacharide (LPS) immunoglobulin (Ig)A were investigated by enzyme-linked immunosorbent assay (ELISA). Total IgA was measured by radial immunodiffusion assay. Synovial fluid-derived mononuclear cells (SFMC) were stimulated with bacterial antigens or SF-conditioned media, and cytokines and IgA were analyzed in the supernatants. IL-17, IL-6 and
i. Reaction of identity: Antigens in two wells are identical (Agl and Ag2). So the reaction with the antibody results in a precisely similar precipitin lines. The precipitin lines dont cross, but form a continuous line. Fusion of the two precipitin lines indicates that the antibody is reacting with epitopes commonly present on the two antigens. ii. Reaction of non-identity: Two non-identical antigens (Agl and Ag3) are in the antigen wells while the antibody well has antibodies to both the antigens. The two antigen-antibody precipitin lines formed differ from each other. Hence the two precipitin lines completely cross (intersect) each other. iii. Reaction of partial identity: The antigenic determinants in the antigens of two wells are partially shared (Agl and Agla). Hence the antibody reacts with both the antigens and forms lines that do not form a complete cross. The precipitin line crosses in only one direction. The extended precipitin line is referred to as a spur. The spur indicates that ...
GPM.1.8.2.0001.15 Gel Immunodiffusion Ministry of Health of the Russian Federation General Pharmacopoeia Monograph Gel immunodiffusion GPM.1.8.2.0001.15 First
Exercise 12: Serological Testing: Activity 2: Comparing Samples with Ouchterlony Double Diffusion Lab Report Pre-lab Quiz Results You scored 100% by
Tseng, C.-E., Buyon, J. P., Kim, M., Belmont, H. M., Mackay, M., Diamond, B., Marder, G., Rosenthal, P., Haines, K., Ilie, V. and Abramson, S. B. (2006), The effect of moderate-dose corticosteroids in preventing severe flares in patients with serologically active, but clinically stable, systemic lupus erythematosus: Findings of a prospective, randomized, double-blind, placebo-controlled trial. Arthritis & Rheumatism, 54: 3623-3632. doi: 10.1002/art.22198 ...
Pulmonary tuberculosis (PTB) is global disease affecting about one third of the worlds population with its attendant mortality and morbidity. Acute phase proteins have been used in monitoring the progression of infections but not in relation to PTB in this environment. The levels of total protein, albumin, α-2-macroglobulin, transferrin, and haptoglobulin were determined in 23 patients with PTB and 17- age / sex matched PTB-free controls using spectrophotometric and immunodiffusion methods respectively. The result showed that α-2-macroglobulin was significantly raised in PTB patients compared with controls ( ...
Alternative methods are being sought to measure the potency of influenza vaccines. Label-free technologies that do not require the use of hemagglutinin (HA)-specific antisera are particularly attractive as the preparation of antiserum delays availability of potency reagents. The objective of these experiments was to evaluate the use of a Corning Epic® label-free method to quantify functional influenza hemagglutinin in rHA preparations. The method was optimized to quantify recombinant HA (rHA) of B/Brisbane/60/2008 (B/BR/08). Fetuin was immobilized onto plates and the change in wavelength of refracted light measured using an Enspire (Perkin Elmer) instrument. The change in wavelength measured in response to addition of rHA of B/BR/08 was proportional to its concentration and was optimal in the presence of native rHA conformations. However, the assay was strain-dependent and did not correlate with HAU measured using turkey red blood cells. The Corning Epic® label-free method is suitable for quantifying
To determine Equine IgG levels our laboratory uses only a standardized radial immunodiffusion test, which gives a more accurate result than many of the semi-quantitative tests available. Results are available by phone or fax. This test requires a plasma sample. ...
Summary The particulate forms of HBsAg were analysed for the presence of HBeAG on their surfaces. By immunodiffusion analysis, anti-HBe did not form precipitin bands with the purified forms of HBsAg and hyperimmune guinea pig antisera to these forms did not react with HBeAg. Lines of non-identity were observed between the HBeAg determinants (e1 and e2) and the Dane particles and filaments isolated from an HBeAg-positive serum. Finally, anti-HBe failed to precipitate the polymerase-positive subpopulation of Dane particles, indicating that anti-HBe has no direct role in virus neutralization.
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Ebbesen, P, Amyloid induction with casein in mice of different ages and investigation for casein antibodies using the single radial diffusion technique. (1971). Subject Strain Bibliography 1971. 857 ...
Human autoantibody against Sm antigen, buffered with 0.01M phosphate, 0.13 M NaCl, 0.02% NaN3. For use in ENA Ouchterlony kit. Shows the Sm precipitate band when tested using double immunodiffusion against rabbit thymus extract (RTE-1000).
Draw blood in a plain red-top tube(s), serum gel tube is acceptable. Spin down and send 1 mL of serum refrigerated in a plastic vial.
a technique used to detect the reaction between an antigen and an antibody by observing the behavior of a combination of such species as they diffuse through a gel ...
M. J. BREITENBACH CO. + MARTIN H. SMITH CO. MANUFACTURERS M. J. BREITENBACH CO. CANCEL VARIETIES USED TO PAY TAX ON $1 SIZE BOTTLE SINGLE LINE SANSERIF COMPANY INITIAL VARIETY SINGLE LINE COMPANY INITIAL VARIETY WITH SERIFS In virtually every grouping of proprietary battleship cancels, one finds a red two-and-one-half cent (RB28) cancelled with the initials M…
I would need to double-check but label should be straight/no formatting/single line text controls while the textarea are rich/with formatting/multi line text controls. Im surprised that carriage return actually work with labels, i would have expected that it made the text disappear from the area for the label (i.e. it would show up if the label control was given more height ...
my newest suspension idea. single line with whoopie on each end.i moved the rings that the chain links attach to,to the inside of the whoopies.now i have an adjustable structural ridge(by loosening the rings knots and moving them)and an easily adjustable whoopie on each end.the buries of the whoppies are no longer making my setup longer,and are under the tarp.no more accidently pulling the loop out of the whoopie. http://www.hammockforums.net/forum/picture.php?albumid=234&pictureid=2596 the
At Thu, 27 Oct 2005 17:06:27 +0200, Alfred M Szmidt wrote: , , When I came to the Hurd in 1997, it was essentially dead. It was , not yet officially declared death, but it was pretty close. , , You must have a skewed view of history, the Hurd was no where near , `essentially dead in 1997. Huh, yeah, it should have been 1998. , on FOO. During the past 5 years the Hurd has seen about as many , changes that it saw during 1996. For the record, you didnt , contribute a single line until 1999. I joined the Hurd in April 1998, not 1997 as I wrongly wrote above. My first post was, ironically, an email to help-hurd with the subject title: Subject: hello, seems that hurd is stuck. Message-ID: ,[email protected], I am not going to argue with you about the meaning of the word dead, but GNU 0.2 was released in June 1997, and was so bug-riddled that you could hardly do anything at all with it except to look at the blinking cursor. I was the first one to report the critical bugs in it, one year after the ...
Looking for online definition of Double Immunodiffusion in the Medical Dictionary? Double Immunodiffusion explanation free. What is Double Immunodiffusion? Meaning of Double Immunodiffusion medical term. What does Double Immunodiffusion mean?
TY - JOUR. T1 - A cohort study of the association between serum immunoglobulin G concentration and preweaning health, growth, and survival in Holstein calves. AU - Pithua, P.. AU - Aly, Sharif S. PY - 2013. Y1 - 2013. N2 - The association between serum IgG concentration and preweaning health performance in Holstein calves were evaluated in a prospective cohort study design framework. Post colostral blood samples were collected from 561 calves and serum IgG concentrations were quantitated using a single radial immunodiffusion assay. Preweaning treatments for diarrhea, pneumonia, omphalitis and frequency of antibiotic therapy plus calf death events were recorded until calves were weaned at approximately 60 d of age. Logistic regression modeling was used to evaluate the association between failure of passive transfer of immunity (FPT, serum IgG , 10g/L) and risk of diarrhea, pneumonia, omphalitis, frequency of antibiotic therapy and death. Calves with FPT were twice as likely to suffer from scours ...
The complex antigenic properties of adenovirus-soluble complete and incomplete hemagglutinins were utilized to study the specificity of adenovirus anti-hemagglutinin sera in immunodiffusion and counterimmunoelectrophoresis tests. Pentons, penton dimers, fiber dimers, and/or dodecons of adenovirus types 2, 3, 5, 7a, 8, and 11 were extensively purified by ion-exchange and exclusion chromatographies. Components were determined to be purified proteins by immunologic, electron microscopic, and polyacrylamide gel electrophoretic criteria. Specific antigenic components were identified by immunoelectrophoresis and by various biologic characteristics. Rabbit antisera prepared against these hemagglutinating components were type-specific in standard serologic, Ouchterlony immunodiffusion, immunoelectrophoretic, and counterimmunoelectrophoretic tests. Counterimmunoelectrophoresis was particularly well suited to a rapid identification and typing of adenovirus isolates for diagnostic purposes.. ...
TY - JOUR. T1 - An immunologic study of the apoproteins of rat lung surfactant. AU - Katyal, S. L.. AU - Singh, Gurmukh. PY - 1979/1/1. Y1 - 1979/1/1. N2 - An immunologic analysis of lung surfactant-associated proteins, designed to detect surfactant-specific protein(s), was performed. Rat lung surfactant was isolated and purified and was used to raise antisera in rabbits. The antisera were found to react with both rat serum and lung surfactant. Antibodies against rat serum proteins were removed from the IgG fraction of the antiserum by means of affinity chromatography using rat serum linked to Sepharose 4B. The specificity of the purified IgG thus prepared (surfactant-specific IgG) was checked by immunodiffusion analysis and by immunoperoxidase and immunofluorescence staining of sections of lungs obtained from adult, newborn, and fetal rats. Immunodiffusion analysis revealed a single precipitin line against rat lung surfactant, but no reactivity with rat serum proteins. After immunoperoxidase ...
Antisera were raised against rods of 17 named Arthrobacter and Aureobacterium strains. Antigenic relationships between these strains, other soil bacteria and new Arthrobacter isolates from several soils were studied, using agglutination, immunodiffusion, immunofluorescence and ELISA techniques. Many of the named Arthrobacter species had common antigens, and there were also common antigens amongst named Arthrobacter strains and many fresh Arthrobacter isolates. Agglutination, ELISA and immunofluorescence tests revealed greater antigenic differences between the named strains than did immunodiffusion tests. Serological similarities between the 17 named strains and the fresh Arthrobacter isolates were calculated using S coefficients. The occurrence of named strains in serogroups based on immunodiffusion data supported the taxonomic scheme for arthrobacters in Bergey's Manual of Systematic Bacteriology. The distribution of soil isolates in serogroups resembled that in groups based on numerical analysis
Background: Trichomoniasis caused by Trichomonas vaginalis, is one of the most commonsexually transmitted parasites in the world, accounting for approximately 170 millioninfections annually. Although the factor behinds such incidence is still not clear,local and systemic host immune response is involved.Objectives: Accordingly, the present research was planned to determine the level ofimmunoglobulins (IgA, IgG and IgM) in sera and vaginal washes of women infectedwith T. vaginalis.Materials and Methods: Fifteen women infected with T. vaginalis (culture confirmed) and15 healthy females were inspected for total level of IgA, IgG and IgM in their seraand vaginal washes by means of single radial immunodiffusion.Results: The total level of IgA, IgG and IgM in serum and vaginal wash of patients and controlsshowed no significant difference, with the exception of IgG, which showed asignificant increased mean in the sera of patients.Conclusion: Humoral immune response is important in controlling T. ...
Evidence exists that C is involved in the coagulation process (Zimmerman et al., J. Exp. Med., No. 6, 1971). It has been reported that C3 (β1C/β1A) is significantly depressed in most patients with DIC (Tomar et al., Thromb. Diath. Haemorrh., 3: 27, 1972). In the present study C3 and C4 were quantitated by single radial immunodiffusion in the plasma of patients suffering mostly from severe septic and traumatic conditions with and without shock. Examinations including detailed coagulation studies were performed on 30 selected patients and 14 showed characteristic signs of DIC. C3 was markedly diminished in 13, C4 in 9 of the 14 DIC patients. In patients with septicemia but without DIC, a larger variation of results was found. In this group, depressions of C3 were observed in 6, and of C4 in 3 of 10 cases and seemed to occur more frequently when the condition was complicated by shock.. ...
Twenty cats with FIP and twenty with FIV were studied. The objective of the present study was to establish the diagnostic value of these two tests in both diseases. Correlation between AGP and Albumin/Globulin ratio (A/G) and CD4/CD8 ratio were analyzed in both groups.. FIV was confirmed by serology and PCR (Polymerase Chain Reaction). FIP was confirmed by histopathology (vasculitis and perivasculitis). In addition to the evaluation of the clinical signs blood chemistry, hematology and other complementary diagnostic methods. CD4/CD8 by flow cytometry and AGP test were performed in order to establish the diagnostic value of these two tests in both diseases. CD4/CD8 ratio was performed with: 8100-01 Sowthern Biotechnology, anti CD4 Vpg 34, Willett, Glasgow University, anti CD8, VPG9, Willett, Glasgow University and Fluorescent antimouse IgG Southern Biotechnology, Becton and Dickinson Facs Scan Citometer. Feline α 1 glycoprotein (AGP) was performed with a radial immunodiffusion test provided by ...
A glycoprotein with an apparent 340,000 mol wt (gp 340K) was isolated from rat kidney saline-soluble extract by ammonium sulfate precipitation, DE 52 ion-exchange cellulose chromatography, concanavalin A affinity column, Sephacryl S-300 gel filtration, and discontinuous polyacrylamide gel electrophoresis (PAGE). The relative purity of gp 340K was examined by double immunodiffusion analysis, disc PAGE, and immunoelectrophoresis. Injection of rabbit gp 340K antiserum into pregnant rats during the organogenetic period induced abnormal embryonic development, fetal growth retardation, and embryonic death. Antiserum against the immunocomplexes isolated by immobilized protein A also produced the same embryotoxic effects. The biologic effects of the antisera appeared to be dose dependent. Defects such as anophthalmia, hydrocephaly, exencephaly, cleft palate, cleft lip, and some cardiovascular anomalies were observed. The most frequently observed anomaly was anophthalmia. Immunofluorescent localization ...
Previous studies demonstrated the cross antigenicity between endothelial and epithelial basement membranes. In the present study we have isolated and characterized chemically and immunologically the antigenic components of basement membranes from the glomerulus, alveolus, and lens capsule. The basement membranes were solubilized by reduction and alkylation and antisera prepared in rabbits. On immunodiffusion two precipitin lines of identity form when antiserum to either reduced and alkylated glomerulus or lens-capsule basement membranes is reacted with both antigens. Reduced and alkylated alveolus basement membrane reacts similarly with both antisera. Upon fractionation of the basement membranes two antigenic components are obtained. One is ...
The reactivity of the antiserum is directed to the Fc and Fab subunits of both subclasses, IgG1 and IgG2. It includes a certain degree of reactivity with other immunoglobulins via the common Fab portion. In immunoelectrophoresis against goat serum a single characteristic precipitin line with a spur is usually obtained, representing primarily IgG2. If the IgG1 level in the sample approaches the concentration of IgG2, it may become visible as a second precipitin line. It does not react with any non-Ig protein in goat serum, as tested by immunoelectrophoresis and double radial immunodiffusion. As unlabelled primary or secondary reagent for indirect detection techniques, to prepare conjugates with markers of the users own choice, to prepare an insoluble immunoaffinity adsorbent or a solid phase antibody reagent by coupling to an artificial carrier and as catching or detection antibody in nonisotopic methodology and solid phase immunochemistry. When applied in any cytochemical or histochemical ...
Glyceraldehyde 3-phosphate dehydrogenases (EC 1.2.1.12 and 1.2.1.13) have been purified from the seed, root, etiolated, and green shoot of peas (Pisum sativum). These enzymes are tetramers of 140,000 daltons, with subunits of 35,000 daltons. The enzymes differ in isoelectric point. The seed enzyme has a pI of 5.1, and the root enzyme has a pI of 4.5. The cytoplasmic enzyme from etiolated shoots is slightly acidic with a pI of 5.7 to 6.1 and is found in two separable forms. The chloroplast enzyme (from green shoots) is most basic with a pI of 8.0.. In immunodiffusion experiments, the seed, root, and cytoplasmic enzymes of the etiolated shoot share antigenic homology, while the chloroplast enzyme does not cross react antigenically with the extra-chloroplast enzymes. The antiserum to the pea chloroplast enzyme did, however, cross react with glyceraldehyde 3-phosphate dehydrogenase purified from the spinach chloroplast. Therefore, the chloroplast enzyme is significantly different from the ...
or by regullar mail to: Mihai Larian Brad, Facultatea de Inginerie, Universitatea de Vest Vasile Goldis, Str. Cocorilor nr. 57, Arad, 310426, Romania. Please send only doc format. Page setup: Paper A4 format, Portrait, Top 1cm, Bottom 1cm, Right 2 cm, Left 2cm, Gutter 0cm, Mirror margins, Header 1cm, Footer 1cm. Please do not insert page numbers or any other text in Header and Footer. Title: Century Gothic 16, All caps, bold, shadow, center, single line. Title should be only in english. Subtitles: Main subtitles (such as Introduction, aterials and methods, Results and Discussion, Conclusions, References) should be Arial 10, All Caps, bold, left, single line. Sub-subtitles should be Arial 10, Sentence case, bold, left, single line. Authors: Arial 10, Title case, bold, center, single line. Full name is required with your given name followed by your surname (i.e. John Hopkins). Authors names should be separated by commas (i.e. John Hopkins, Jane Harper). For each author, there has to be an ...
Molybdenum EndPoint ID Test Kits help simplify the testing process. Each kit is packaged in a durable plastic case and includes all items to complete approximately 60-75 tests.. ...
Adiga, PR (1981) Biochemical And Immunological Studies On Riboflavin-Carrier Protein (Rcp) Essential For Fetal Survival In The Pregnant Rat - A New Approach To Fertility-Control. In: Indian Journal of Biochemistry & Biophysics, 18 (4). pp. 36-37. Full text not available from this repository ...
A double diffusion test system for the titering and semi-quantitative determination of autoantibodies to Jo-1 in human serum. The presence of these a...
A double diffusion test system for the screening and semi-quantitative determination of autoantibodies to SS-A/SS-B in human serum. The presence of t...
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When it comes to growing intestines, the first inch is the hardest -, especially in a Petri dish. Scientists at Cincinnati Childrens Hospital Medical Center have met that benchmark: they recently reported in Nature Medicine that they had grown a piece of gut-nerves, muscles and all& ...
Goat anti Mouse IgG antibody recognizes both the heavy and light chains of mouse IgG. Immunoelectrophoresis revealed a single precipitin a
, Mouse IgG2a antibody, GTX29163, Applications: ELISA, ID, IE, WB; ELISA, Immunodiffusion, Immunoelectrophoresis, Western Blot (WB); CrossReactivity: Mouse
This is very nice to get this all at a glance, but the ObjDef column is limited. I can widen the column in the grid, but only so far. And the contents dont contain any of the newline characters… its just one looonnngggg string of text that I cant read. I could copy/paste the contents into Excel, but again, it will just be a single line of text with no newline characters. And even so, SSMS will not output any more than 65536 characters in a column in a grid result window, so we may not get the full code anyway ...
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You can use the Netfw.inf file to implement custom Windows Firewall settings when performing an interactive installation of Windows XP SP2 from a central file share that contains all of the Windows XP SP2 files.. The default contents of the Netfw.inf file are the following:. Note Some parts of the following code snippet have been displayed in multiple lines only for better readability. These should be entered in a single line.. ...
A university cafeteria line in the student center is a self-serve facility in which students select the food items they want and then form a single line to pay the cashier. Students arrive at a rate of about four per minute.
mutually exclusive choices of a required item are grouped with parentheses: ( ) The brackets, vertical bars, and parentheses mentioned above are merely symbolic and should not be included in the typed command. All commands can be truncated to unique strings. Keyword truncation is not allowed unless a commands manual page states otherwise. Multiple commands can be combined on a single line, separated by semicolons. This may be useful because the display is not redrawn until the last command in a semicolon-separated series has been executed; that is, multiple graphical changes such as changes in representation, color, and display state can be made to appear simultaneously instead of one by one. ...
Functional UI Testing. You dont need to write a single line of code to add conditional logic, invoke JavaScript and desktop commands.
Wufoo is a web form builder that helps anyone create beautiful forms, surveys and invitations without writing a single line of code.
A quote can be a single line from one character or a memorable dialog between several characters. Please make your quotes accurate. Quotes will be submitted for approval by the RT staff.. ...
Wufoo is a web form builder that helps anyone create beautiful forms, surveys and invitations without writing a single line of code.
Wufoo is a web form builder that helps anyone create beautiful forms, surveys and invitations without writing a single line of code.
Wufoo is a web form builder that helps anyone create beautiful forms, surveys and invitations without writing a single line of code.
Instructions for Authors General Information--submission Single line spacing for manuscripts is preferred for this journal.Please place images and tables near ...
for(i in 1:num_folds){ train_ids,- folds[[i]] test_ids,-all_participants[!(all_participants%in%folds[[i]])] #generate train/test train,-subset(aids,id%in%train_ids) test,-subset(aids,id%in%test_ids) #fit models aids_lm ,- lm(formula=CD4~obstime+drug+gender+prevOI+AZT,data=train) aids_lmm ,-lmer(formula=CD4~obstime+drug+gender+prevOI+AZT+(1,id),data=train) test_participants ,- unique(test[[id]]) for(participant in test_participants){ prediction_subset ,- subset(test,id==participant) #predict y_pred_lm ,- predict(aids_lm,newdata=prediction_subset) y_pred_lmm ,- predict(aids_lmm,newdata=prediction_subset,allow.new.levels=TRUE) y_true ,- prediction_subset[[CD4]] MAE_within_subjects_lm,- c(MAE_within_subjects_lm,mean_absolute_error(y_pred_lm,y_true)) MAE_within_subjects_lmm,- c(MAE_within_subjects_lmm,mean_absolute_error(y_pred_lmm,y_true)) MSE_within_subjects_lm,-c(MSE_within_subjects_lm,mean_squared_error(y_pred_lm,y_true)) ...
The test uses immunodiffusion. A strip of filter paper impregnated with diphtheria antitoxin is buried just beneath the surface ...
Serologic specificity demonstrated by immunodiffusion. Related to Mycobacterium fortuitum: can be distinguished by its intense ...
The findings were confirmed by immunodiffusion. Lines of identity were observed when antigen 5s, or phospholipases, from the ...
David-West, Tam S (1966). "The standardisation of some immunodiffusion systems in agar gel". Journal of Pathology and ...
In addition, virus antigen can be detected double immunodiffusion. Postmortem examination may reveal a variety of clinical ...
Differential characteristics 5 species-specific antigens, demonstrable in immunodiffusion tests. Not assumed to be pathogenic. ...
Coleman RM, Kaufman L (February 1972). "Use of the immunodiffusion test in the serodiagnosis of aspergillosis". Applied ...
Ridell, M (March 1983). "Immunodiffusion analyses of Mycobacterium farcinogenes, Mycobacterium senegalense and some other ...
Double immunodiffusion (DID) and counterimmunoelectrophoresis (CIEP), two forms of gel-based techniques, are used to gain ... Bunn, C.; Kveder, T. (1996). "Counterimmunoelectrophoresis and immunodiffusion for the detection of antibodies to soluble ... double immunodiffusion (DID) [1] and counterimmunoelectrophoresis (CIEP). Both of these techniques require the precipitation of ... which are screened for by Ouchterlony double immuno diffusion techniques and confirmed by immunoblotting. On anti-nuclear ...
Single radial immunodiffusion assay (SRID), also known as the Mancini method, is a protein assay that detects the amount of ... specific viral antigen by immunodiffusion in a semi-solid medium (e.g. agar). The medium contains antiserum specific to the ... ". "Pierce Protein Biology". Rodda, S.J.; Gallichio, H.A.; Hampson, A.W (1981). "The Single Radial Immunodiffusion Assay ...
Using ELISA is more specific than using a Western blot or Arc2 immunodiffusion. Proteases secreted by F. hepatica have been ...
Other detection methods include the use of enzyme-linked-immuno-sorbent-assays and radial immunodiffusion. Different analytical ...
The virus can be detected with ELISA, immunodiffusion tests, or a nucleic acid hybridization assay. The Cymbidium mosaic virus ...
Gel immunodiffusion is commonly used in endemic areas, diagnoses 95% of cases with high specificity. Complement fixation allows ...
... double immunodiffusion, crossed immunoelectrophoresis, and human specific IgE serologic surveys". Annals of Allergy, Asthma & ... double immunodiffusion, crossed immunoelectrophoresis, and human specific igE serologic surveys. (Abstract)". Annals of Allergy ...
Differential characteristics Characterised by the possession of 6 species-specific antigens demonstrable in immunodiffusion ...
Diagnosis relies on agar gel immunodiffusion, ELISA (enzyme linked immunosorbent assay) and PCR (polymerase chain reaction). ...
Malmquist, W. A.; Van der Maaten, M. J.; Boothe, A. D. (January 1969). "Isolation, immunodiffusion, immunofluorescence, and ...
They can be confirmed using virus isolation, complement fixation, ELISA, immunodiffusion or histopathology (following ... agar gel immunodiffusion, indirect immunofluorescence (IIF), and virus neutralization (VN). Virus neutralization identifies ...
The tests adopted were the Coggins Test (also known as the Agar Gel Immuno-Diffusion (AGID) Test). In 1995, the Blood Typing ...
Other detection methods include use of enzyme-linked-immuno-sorbent-assays in vitro and radial immunodiffusion. Alpha-1 ...
Research based on immunodiffusion technique and comparison of protein and DNA sequences revealed the close relationships ...
... immunofluorescence test or agar gel immunodiffusion test. They, however, can be isolated by using the HinDill restriction ...
India Ouchterlony double immunodiffusion, an immunological technique named after Örjan Ouchterlony This disambiguation page ...
"Immunodiffusion". ScienceDirect. Elsevier B.V. Archived from the original on 2017-05-02. Retrieved 2017-05-19.. (2) "Photograph ... "Photograph of radial immunodiffusion in a Petri dish". Edvotek, Inc. 2017. Archived from the original (photograph) on 2017-08- ... Petri dishes are also used for cell cultivation of isolated cells from eukaryotic organisms, such as in immunodiffusion studies ... of double immunodiffusion in a Petri dish" (photograph). Retrieved 2017-05-15.. (3) " ...
Immunodiffusion, a modified direct complement-fixation test, and enzyme-linked immunosorbent assay also have been used ...
The Coggins test (agar immunodiffusion) is a sensitive diagnostic test for equine infectious anemia developed by Dr. Leroy ...
Histology, virus isolation, electron microscopy, immunoperoxidase and immunofluorescent staining, immunodiffusion, complement ...
... immunodiffusion) and specific lipid patterns. A numerical taxonomy comparison showed close relationship to Mycobacterium ...
immunodiffusion (plural immunodiffusions). *a technique used to detect the reaction between an antigen and an antibody by ... Retrieved from "https://en.wiktionary.org/w/index.php?title=immunodiffusion&oldid=44714844" ...
The low EEO and high gel strength is specifically selected and tested for immunoelectrophoresis and immunodiffusion ... The low EEO and high gel strength is specifically selected and tested for immunoelectrophoresis and immunodiffusion ...
The backbone of agarose is that of alternating ß-D-galactapyranose and 3,6-anhydro-a-L-galactapyranose linked together by glycosidic bonds. Sulfate ester, methoxyl and carboxyl groups in various amounts and combinations are attached to the backbone.
LSU Health Sciences Centers Department of Microbiology, Immunology and Parasitology is located in New Orleans, LA, and serves the scientific community by teaching and training graduate, medical, dental, and dental hygiene students in all aspects of microbiology, immunology and parasitology.
... immunodiffusion explanation free. What is immunodiffusion? Meaning of immunodiffusion medical term. What does immunodiffusion ... Looking for online definition of immunodiffusion in the Medical Dictionary? ... single radial immunodiffusion, Radial immunodiffusion, Double Immunodiffusion. immunodiffusion. [im″u-no-dĭ-fu´zhun] the ... immunodiffusion. Also found in: Dictionary, Thesaurus, Legal, Encyclopedia, Wikipedia.. Related to immunodiffusion: ...
Associations of Immunodiffusion with chemical compounds. *Radial double immunodiffusion experiments using antiserum to C1q, C1r ... C-reactive protein (CRP) was assayed by radial immunodiffusion [30].. *Double immunodiffusion precipitation revealed that ALDH1 ... Disease relevance of Immunodiffusion. *C3b inactivator in the rheumatic diseases. Measurement by radial immunodiffusion and by ... Biological context of Immunodiffusion. *This result was confirmed by an immunodiffusion test that demonstrated the absence of ...
Draw blood in a plain red-top tube(s), serum gel tube is acceptable. Spin down and send 1 mL of serum refrigerated in a plastic vial.
Immunodiffusion and immunoelectrophoresis are useful techniques for these purposes.. If antibody and antigen are present in ... This is the basis of both immunodiffusion and immunoelectrophoresis. In both techniques a gradient of antigen, or of antibody ... This technique, immuno-electrophoresis, is much faster than immuno-diffusion, and has been applied in a variety of geometries, ... Of the common patterns of wells containing antibody antiserum (Ab) and antigen samples (Ag) used in immunodiffusion, the system ...
Other Fungal Immunodiffusion Plates, 1 Series Fungal Immunodiffusion Plates, 4 Series Histoplasma Antigen for Fungal ... Immunodiffusion. Immunodiffusion & Complement Fixation products, including aspergillus, histoplasma, Coccidioides, and Endo- ... Immunodiffusion Coccidioides "F" Antigen for Fungal Immunodiffusion (1 ml) Coccidioides "F" Antigen for Fungal Immunodiffusion ... for Fungal Immunodiffusion Anti-Coccidioides "F" Control Serum (Goat or Human or Rabbit) for Fungal Immunodiffusion Anti- ...
What is Double Immunodiffusion? Meaning of Double Immunodiffusion medical term. What does Double Immunodiffusion mean? ... Looking for online definition of Double Immunodiffusion in the Medical Dictionary? Double Immunodiffusion explanation free. ... redirected from Double Immunodiffusion). Also found in: Acronyms. gel dif·fu·sion pre·cip·i·tin tests in two di·men·sions. ... Double Immunodiffusion , definition of Double Immunodiffusion by Medical dictionary https://medical-dictionary. ...
immunodiffusion answers are found in the Tabers Medical Dictionary powered by Unbound Medicine. Available for iPhone, iPad, ... immuno- + diffusion] A test method in which an antigen and antibody are placed in a gel, where they diffuse toward each other. ... immunodiffusion is a topic covered in the Tabers Medical Dictionary. To view the entire topic, please sign in or purchase a ... "Immunodiffusion." Tabers Medical Dictionary, 23rd ed., F.A. Davis Company, 2017. Tabers Online, www.tabers.com/tabersonline/ ...
... of precipitating antibodies and standardization of streptococcus group B antisera by the reverse radial immunodiffusion ... of precipitating antibodies and standardization of streptococcus group B antisera by the reverse radial immunodiffusion ...
Photograph of precipitin circles in a Petri dish during radial immunodiffusion. "Diffusion Patterns". Immunodiffusion ... radial immunodiffusion or Mancini method) Double diffusion in two dimensions (Ouchterlony double immunodiffusion) "Use of the ... Immunodiffusion is a diagnostic test which involves diffusion through a substance such as agar which is generally soft gel agar ... Photographs of Ouchterlony immunodiffusion patterns showing stained precipitin lines of full identity, partial identity and non ...
Radial immunodiffusion (RID) or Mancini method, Mancini immunodiffusion or single radial immunodiffusion assay, is an ... Stanley J (2002). "Chapter 12: Precipitation: Single Radial Immunodiffusion: Laboratory Technique 12-1: Radial Immunodiffusion ... Introductory video demonstrating radial immunodiffusion technique (3:43 minutes). "Radial Immunodiffusion (Mancini Technique ... Davis NC, Ho M (1976). "Chapter 2: Quantitation of Immunoglobulins: Radial Immunodiffusion". In Rose N, Friedman H (eds.). ...
... * Other Fungal Immunodiffusion Plates, 1 Series Fungal Immunodiffusion Plates, 4 ... Antigen for Fungal Immunodiffusion (1 ml) Coccidioides "F" Antigen for Fungal Immunodiffusion (5 ml) Coccidioides "TP" Antigen ... for Fungal Immunodiffusion Anti-Coccidioides "F" Control Serum (Goat or Human or Rabbit) for Fungal Immunodiffusion Anti- ... LeadCare® ImmunoCard STAT ImmunoCard Premier TRU Merifluor Immunodiffusion Latex Agglutination TruQuick * Proficiency and ...
The CPT codes provided are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payer being billed ...
Detection of paracoccidioidomycosis circulating antigen by the immunoelectroosmophoresis-immunodiffusion technique. Preliminary ... R. E.; GEZUELE, E.; GIMENEZ, A. C. de; PENA, M. I. & MACKINNON, J. E. - Immunoelectroosmophoresis immunodiffusion in ... Detection of paracoccidioidomycosis circulating antigen by the immunoelectroosmophoresis-immunodiffusion technique: preliminary ... Detection of paracoccidioidomycosis circulating antigen by the immunoelectroosmophoresis-immunodiffusion technique: preliminary ...
Studies of echovirus type 6 antigens in immunodiffusion. 3. Antibody response against echovirus 6 antigens in human infections ... Studies of echovirus type 6 antigens in immunodiffusion. 1. Antigen composition of native and heated virus preparations. Acta ... Studies of Echovirus type 6 antigens in immunodiffusion. 2. Immunoelectrophoretic investigation. Acta Pathologica et ... Studies of echovirus type 6 antigens in immunodiffusion. 3. Antibody response against echovirus 6 antigens in human infections ...
... DSpace/Manakin Repository. ... as determined by the radial immunodiffusion technique, were not significantly different. show less ... as determined by radial immunodiffusion and with phenotype determination on polyacrylamide gel electrophoresis have been re- ...
Key words: Mareks disease, Herpesvirus of turkey, Polymerase chain reaction, Agar gel immunodiffusion test. ... Comparision of Polymerase Chain Reaction and Agar Gel Immunodiffusion test in Detection of Mareks Disease Virus ...
On the basis of immunoglobulin G concentration assessed using radial immunodiffusion, 20.9% of colostrum samples were of low ... Evaluation of colostrum quality in the Czech Republic using radial immunodiffusion and different types of refractometers. ... Evaluation of colostrum quality in the Czech Republic using radial immunodiffusion and different types of refractometers. ... Evaluation of colostrum quality in the Czech Republic using radial immunodiffusion and different types of refractometers. ...
Find out information about immunodiffusion. A serological procedure in which antigen and antibody solutions are permitted to ... diffuse toward each other through a gel matrix; interaction is manifested... Explanation of immunodiffusion ... single radial immunodiffusion, Radial immunodiffusion, Double Immunodiffusion immunodiffusion. [¦im·yə·nō·də′fyü·zhən] ( ... immunodiffusion. Also found in: Dictionary, Thesaurus, Medical, Legal, Wikipedia.. Related to immunodiffusion: ...
... of immunodiffusion testing. 2. Determine the concentration of an immunoprotein by preparation of a reference curve and ... Retrieved from " https: Radial immunodiffusion RID or Mancini method, Mancini immunodiffusion or single radial immunodiffusion ... Chromatin immunoprecipitation Immunodiffusion Ouchterlony double immunodiffusion Radial immunodiffusion Immunoelectrophoresis ... Immunoprecipitation Chromatin immunoprecipitation Immunodiffusion Ouchterlony double immunodiffusion Radial immunodiffusion ...
Agar gel immunodiffusion test for diagnosis of clinical paratuberculosis in cattle. Author(s) Sherman, D. M.. Markham, R. J. ... Agar gel immunodiffusion test for diagnosis of clinical paratuberculosis in cattle. Primary tabs. *View(active tab) ... Show moreDuring a 19-month period, the agar gel immunodiffusion (AGID) test was used as an aid in differential diagnosis of ... During a 19-month period, the agar gel immunodiffusion (AGID) test was used as an aid in differential diagnosis of ...
... avian paramyxoviruses consisting of ten reference strains and seven recent isolates were classified by immunodiffusion into six ... Classification of Avian Paramyxoviruses by Immunodiffusion on the Basis of Antigenic Specificity of their M Protein Antigens * ... avian paramyxoviruses consisting of ten reference strains and seven recent isolates were classified by immunodiffusion into six ...
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Mancini immunodiffusion, Mancini method, Radial immunodiffusion, Radial Immunodiffusion Advantages, Radial Immunodiffusion ... Radial Immunodiffusion Objectives, Radial Immunodiffusion Principle, Radial Immunodiffusion Procedure, Radial Immunodiffusion ... Mancini immunodiffusion Radial Immunodiffusion- Objectives, Principle, Procedure, Results, Applications, Advantages and ... Immuno-diffusion is a technique for the detection or measurement of antibodies and antigens by their precipitation which ...
Immunodiffusion of Antigen or Antibodies ! Immunodiffusion refers to the movement of antigen or antibody or both antigen and ... Double immunodiffusion can also be used for semi quantitative analysis of antigen or antibody (Fig. 27.3): ... Single Immunodiffusion: Either antigen or antibody remains fixed and the other reactant moves (Diffusion technique of Oudin). ... Single Radial Immunodiffusion: Mancini introduced this novel technique for accurate quantitation of antigens. The antibody is ...
Order your next veterinary HISTOPLASMA ANTIBODY IMMUNODIFFUSION detection test from MiraVet Dx: a trusted fungal infection ... The Histoplasma Antibody Immunodiffusion test is used for the in vitro determination of precipitating antibodies to Histoplasma ...
GPM.1.8.2.0001.15 Gel Immunodiffusion Ministry of Health of the Russian Federation General Pharmacopoeia Monograph Gel ... Gel immunodiffusion GPM.1.8.2.0001.15. First Edition. The present General Pharmacopoeia Monograph applies to the method used ... Immunodiffusion specificity is confirmed by the presence of a precipitation line of the standard reagent and the respective ... The principle of the gel immunodiffusion method is that a precipitate (appearing as precipitation lines) forms as a result of ...
  • Chromatin immunoprecipitation Immunodiffusion Ouchterlony double immunodiffusion Radial immunodiffusion Immunoelectrophoresis Counterimmunoelectrophoresis. (berneice.space)
  • Double immunodiffusion for semi-quantitative analysis of antigen. (yourarticlelibrary.com)
  • The purpose of this work was to evaluate two serological assays: double immunodiffusion (DI) and immunoblotting (IB) in immunodiagnosis of paracoccidioidomycosis (PCM). (scielo.br)
  • Radial immunodiffusion (RID) or Mancini method, Mancini immunodiffusion or single radial immunodiffusion assay, is an immunodiffusion technique used in immunology to determine the quantity or concentration of an antigen in a sample. (wikipedia.org)
  • The figures below show different variations of the immunodiffusion assay. (nationaldiagnostics.com)
  • However more sensitive and specific methods like enzyme-linked immunoabsorbent assay (ELISA) and radio immunoassay (RIA) have almost replaced the immunodiffusion methods. (yourarticlelibrary.com)
  • Silver-enhanced radial immunodiffusion assay of plasma apolipoproteins. (jax.org)
  • Silver-staining of immunoprecipitates extends the sensitivity of the radial immunodiffusion assay by tenfold. (jax.org)
  • Sera from fasting subjects were used to determine, by means of quantitative immunodiffusion assay, the level of each individual protein. (elsevier.com)
  • The reference values of the three main types of serum haptoglobin Hp 1-1, Hp 2-1, and Hp 2-2, as determined by radial immunodiffusion and with phenotype determination on polyacrylamide gel electrophoresis have been re-evaluated for both sexes. (uu.nl)
  • Approximately 10 days after each injection, a blood sample was collected, the serum was separated and tested for anti-[alpha]2M antibody activity by immunodiffusion (ID) and immunoelectrophoresis (IEP), prior and after its absorbance to normal rat serum. (thefreedictionary.com)
  • The immunodiffusion methods are used for qualitative and quantitative analysis of serum proteins. (yourarticlelibrary.com)
  • Immunodiffusion specificity is confirmed by the presence of a precipitation line of the standard reagent and the respective serum precipitating the species specific proteins. (pharmacopoeia.ru)
  • Identification from crossed electro-immunodiffusion patterns of serum. (semanticscholar.org)
  • An attempt to identify the individuality from crossed electro-immunodiffusion (CEID) patterns of serum was made by using coefficient of variation (CV), the ratio of standard deviation to mean of ratios of area in couples of corresponding peaks in the CEID patterns. (semanticscholar.org)
  • Radial immunodiffusion, a variation of the agar precipitation technique, is used in clinical immunology for the detection and quantitation of all classes of immunoglobulins, complement, ceruplasmin, transferrin, and other serum components. (lsuhsc.edu)
  • Measurement of neonatal equine immunoglobulins for assessment of colostral immunoglobulin transfer: Comparison of single radial immunodiffusion with the zinc sulfate turbidity test, serum electrophoresis, etc. (elsevier.com)
  • Fingerprint Dive into the research topics of 'Measurement of neonatal equine immunoglobulins for assessment of colostral immunoglobulin transfer: Comparison of single radial immunodiffusion with the zinc sulfate turbidity test, serum electrophoresis, etc. (elsevier.com)
  • Agostoni, A, Vergani, C & Lomanto, B 1967, ' Characterization of serum proteins by thin-layer gel filtration combined with immunodiffusion ', The Journal of Laboratory and Clinical Medicine , vol. 69, no. 3, pp. 522-529. (elsevier.com)
  • An agar gel immunodiffusion (AGID) test using gp51/gp30T- as the antigen for the detection of BLV antibodies in serum was developed and compared to traditional AGID, which uses wild type BLV antigen derived from fetal lamb kidney cells. (bvsalud.org)
  • The affinity purified antibodies react only with the IgA in serum and secretions by immunodiffusion and IEP techniques. (abcam.com)
  • A study was designed to determine whether quantitative determinations could be made of organophosphate exposure by determining the ratio of serum cholinesterase activity to the amount of total butyrylcholinesterase (BCHE) protein determined by immunodiffusion. (cdc.gov)
  • Immunodiffusion is a diagnostic test which involves diffusion through a substance such as agar which is generally soft gel agar (2%) or agarose (2%), used for the detection of antibodies or antigen. (wikipedia.org)
  • A reverse radial immunodiffusion test, in which antigen is incorporated in the agar, can be used to quantitate the amount of antibody in a sample. (thefreedictionary.com)
  • Detection of paracoccidioidomycosis circulating antigen by the immunoelectroosmophoresis-immunodiffusion technique. (scielo.br)
  • Immunodiffusion refers to the movement of antigen or antibody or both antigen and antibody molecules in a support medium by diffusion. (yourarticlelibrary.com)
  • The principle of the gel immunodiffusion method is that a precipitate (appearing as precipitation lines) forms as a result of the interaction between an antigen and an antibody taken in an equivalent ratio. (pharmacopoeia.ru)
  • The Candida Immunodiffusion test is an FDA approved procedure to detect precipitating antibodies to both Candida cytoplasmic and mannan antigen fractions. (acromios.eu)
  • One of these, a heat-labile antigen which participates in the immunodiffusion reaction corresponding to the complement fixation reaction (IDCF), has been characterized as a 110-kDa native protein that, when subjected to reducing conditions and heat, yields a 48-kDa component. (elsevier.com)
  • Johnson, SM & Pappagianis, D 1992, ' The coccidioidal complement fixation and immunodiffusion-complement fixation antigen is a chitinase ', Infection and Immunity , vol. 60, no. 7, pp. 2588-2592. (elsevier.com)
  • Guinea pig antiserum containing known quantities of antibody to foot-and-mouth disease virus (140S antigen) and virus protein subunit (12S antigen) was used as a standard in radial immunodiffusion (RID) analyses for determining the antibody content of other antisera. (jimmunol.org)
  • Unlike the conventional radial immunodiffusion , the antigen ( IgG ) is held immobile in the gel while the antibody (Anti IgG ) diffuses radially from the well (7 microliters) and the diameter of the resulting immuneprecipitates after immunodiffusion at 4 degrees C for 24 hr, represents a linear correlation with the antibody titre. (bvsalud.org)
  • During a 19-month period, the agar gel immunodiffusion (AGID) test was used as an aid in differential diagnosis of paratuberculosis in 51 cattle with a history of chronic weight loss and/or chronic diarrhea. (islandscholar.ca)
  • Immunodiffusion and immunoelectrophoresis are useful techniques for these purposes. (nationaldiagnostics.com)
  • This is the basis of both immunodiffusion and immunoelectrophoresis. (nationaldiagnostics.com)
  • verrucosum were antigenically studied in an attempt to identify them by different immunological procedures, i.e. immunodiffusion, two-dimensional immunoelectrophoresis, reversed phase high performance liquid chromatography and hybridoma techniques. (springer.com)
  • Photograph of precipitin circles in a Petri dish during radial immunodiffusion. (wikipedia.org)
  • Photographs of Ouchterlony immunodiffusion patterns showing stained precipitin lines of full identity, partial identity and non-identity. (wikipedia.org)
  • Of the 44 patients, 37 (84%) received a diagnosis by using [greater than or equal to] 1 of the following serologic tests for Coccidioides exposure: complement fixation, immunodiffusion , or tube precipitin. (thefreedictionary.com)
  • Differing methodology and equations used in quantitating immunoglobulins by radial immunodiffusion--a comparative evaluation of reported and commercial techniques" (PDF). (wikipedia.org)
  • However, in contrast to the data available in the literature, the reference values of haptoglobin for men and for women, as determined by the radial immunodiffusion technique, were not significantly different. (uu.nl)
  • The two routine antibody detection methodologies are complement fixation (CF) and immunodiffusion (ID), because of convenience, availability, and accuracy of these assays. (biomedcentral.com)
  • Immunodiffusion and complement fixation (CF) methods can detect coccidioidal immunoglobulin G (IgG). (medscape.com)
  • Studies of echovirus type 6 antigens in immunodiffusion. (eurekamag.com)
  • Radial immunodiffusion (RID) [8, 9, 10, 11] is used extensively for the quantitative estimation of antigens. (thefreedictionary.com)
  • Seventeen avian paramyxoviruses consisting of ten reference strains and seven recent isolates were classified by immunodiffusion into six species on the basis of the antigenic specificity of their M protein (MP) antigens. (microbiologyresearch.org)
  • The objectives were to determine the immunoglobulin G concentration of colostrum in Czech dairy cows, to compare refractometer results with results achieved using the radial immunodiffusion and to evaluate the reliability of three types of refractometers and recommend the best solution for the evaluation of colostrum quality. (vuzv.cz)
  • On the basis of immunoglobulin G concentration assessed using radial immunodiffusion, 20.9% of colostrum samples were of low quality (immunoglobulin G (vuzv.cz)
  • As early as 1949, Orjan Ouchterlony used antibodies as reagents for immunodiffusion methods (26), but the major breakthrough was with the application of RIA, which was developed by Solomon Berson and Rosalyn Yalow and for which Yalow was awarded the Nobel Prize. (thefreedictionary.com)
  • The Histoplasma Antibody Immunodiffusion test is used for the in vitro determination of precipitating antibodies to Histoplasma sp. (miravistavets.com)
  • Immunodiffusion test result was positive for all 5 patients. (thefreedictionary.com)
  • The isolates were confirmed as nontoxigenic C diphtheriae by the California State Department of Public Health Microbial Diseases Laboratory (Berkeley, CA), using both PCR and the Elek immunodiffusion test. (thefreedictionary.com)
  • The Immunodiffusion test confirms late stage P.C.C. when antibody levels have risen significantly. (acromios.eu)
  • Reverse Single Radial Immunodiffusion (SRID) for estimating titre of anti IgG antisera is reported. (bvsalud.org)
  • The purified toxin was highly specific for antisera raised in rabbits against the purified toxin, as revealed by immunodiffusion and immunoblot assays. (thefreedictionary.com)
  • The figure above illustrates a principle underlying the usefulness of immunodiffusion, that with shifts in antibody concentration, a corresponding shift in the region of precipitation will occur. (nationaldiagnostics.com)
  • Describe the principle(s) of immunodiffusion testing. (berneice.space)
  • Development of an enzyme?linked immunoassay for the quantitation of influenza haemagglutinin: an alternative method to single radial immunodiffusion. (essayhub.net)
  • 2017. https://www.tabers.com/tabersonline/view/Tabers-Dictionary/733404/all/immunodiffusion. (tabers.com)
  • PENA, M. I. & MACKINNON, J. E. - Immunoelectroosmophoresis immunodiffusion in paracoccidioidomycosis. (scielo.br)
  • paracoccidioidomycosis, immunodiffusion, immunoblotting. (scielo.br)
  • Immunodiffusion studies of morphologically similar dermatophyte species. (springer.com)
  • The immunodiffusion method is widely used in clinical practice and it is based on the precipitation of the anti-M and anti-H antibodies. (thefreedictionary.com)
  • C3 activator, C1-INH, IgA, IgG and IgM were estimated by the single radial immunodiffusion method of Salimonu et al. (thefreedictionary.com)
  • The silver-enhanced radial immunodiffusion method is readily adapted from the standard method, simple and inexpensive to perform, and does not require costly instrumentation. (jax.org)
  • A 7 well, EIA immunodiffusion template, is used to form well patterns. (drugs.com)
  • For example, it can accommodate standard pour, spiral or surface inoculated plates, as well as large bioassay and Single Radial Immunodiffusion (SRD) plates. (thefreedictionary.com)
  • the principles of single-radial-immunodiffusion (3). (berneice.space)
  • Reverse single radial immunodiffusion for estimation of titre of anti IgG antibody. (bvsalud.org)