Immunodiffusion
Immunoelectrophoresis
A technique that combines protein electrophoresis and double immunodiffusion. In this procedure proteins are first separated by gel electrophoresis (usually agarose), then made visible by immunodiffusion of specific antibodies. A distinct elliptical precipitin arc results for each protein detectable by the antisera.
Immune Sera
Complement Fixation Tests
Serologic tests based on inactivation of complement by the antigen-antibody complex (stage 1). Binding of free complement can be visualized by addition of a second antigen-antibody system such as red cells and appropriate red cell antibody (hemolysin) requiring complement for its completion (stage 2). Failure of the red cells to lyse indicates that a specific antigen-antibody reaction has taken place in stage 1. If red cells lyse, free complement is present indicating no antigen-antibody reaction occurred in stage 1.
Cross Reactions
Nephelometry and Turbidimetry
Chemical analysis based on the phenomenon whereby light, passing through a medium with dispersed particles of a different refractive index from that of the medium, is attenuated in intensity by scattering. In turbidimetry, the intensity of light transmitted through the medium, the unscattered light, is measured. In nephelometry, the intensity of the scattered light is measured, usually, but not necessarily, at right angles to the incident light beam.
Hemagglutination Tests
Coccidioidomycosis
Infection with a fungus of the genus COCCIDIOIDES, endemic to the SOUTHWESTERN UNITED STATES. It is sometimes called valley fever but should not be confused with RIFT VALLEY FEVER. Infection is caused by inhalation of airborne, fungal particles known as arthroconidia, a form of FUNGAL SPORES. A primary form is an acute, benign, self-limited respiratory infection. A secondary form is a virulent, severe, chronic, progressive granulomatous disease with systemic involvement. It can be detected by use of COCCIDIOIDIN.
Equine Infectious Anemia
Rabbits
Leukemia Virus, Bovine
Immunoglobulin G
Enzootic Bovine Leukosis
Pneumonia, Progressive Interstitial, of Sheep
Antigen-Antibody Reactions
Paracoccidioides
Histoplasma
Evaluation Studies as Topic
Enzyme-Linked Immunosorbent Assay
An immunoassay utilizing an antibody labeled with an enzyme marker such as horseradish peroxidase. While either the enzyme or the antibody is bound to an immunosorbent substrate, they both retain their biologic activity; the change in enzyme activity as a result of the enzyme-antibody-antigen reaction is proportional to the concentration of the antigen and can be measured spectrophotometrically or with the naked eye. Many variations of the method have been developed.
Pythium
Histoplasmosis
Electrophoresis, Disc
Electrophoresis in which discontinuities in both the voltage and pH gradients are introduced by using buffers of different composition and pH in the different parts of the gel column. The term 'disc' was originally used as an abbreviation for 'discontinuous' referring to the buffers employed, and does not have anything to do with the shape of the separated zones.
Electrophoresis, Polyacrylamide Gel
Goats
Chromatography, Gel
Counterimmunoelectrophoresis
Blastomycosis
A fungal infection that may appear in two forms: 1, a primary lesion characterized by the formation of a small cutaneous nodule and small nodules along the lymphatics that may heal within several months; and 2, chronic granulomatous lesions characterized by thick crusts, warty growths, and unusual vascularity and infection in the middle or upper lobes of the lung.
Infectious Anemia Virus, Equine
A species of LENTIVIRUS, subgenus equine lentiviruses (LENTIVIRUSES, EQUINE), causing acute and chronic infection in horses. It is transmitted mechanically by biting flies, mosquitoes, and midges, and iatrogenically through unsterilized equipment. Chronic infection often consists of acute episodes with remissions.
Radioimmunoassay
Classic quantitative assay for detection of antigen-antibody reactions using a radioactively labeled substance (radioligand) either directly or indirectly to measure the binding of the unlabeled substance to a specific antibody or other receptor system. Non-immunogenic substances (e.g., haptens) can be measured if coupled to larger carrier proteins (e.g., bovine gamma-globulin or human serum albumin) capable of inducing antibody formation.
Amino Acids
Immunochemistry
Fluorescent Antibody Technique
Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
Horses
Cattle Diseases
Immunoelectrophoresis, Two-Dimensional
Serotyping
Agglutination Tests
Cattle
Precipitin Tests
Pregnancy-Specific beta 1-Glycoproteins
Entomophthora
Brucellosis, Bovine
Immunoglobulin M
Paracoccidioidomycosis
Immunoglobulin A
Immunoassay
Species Specificity
The restriction of a characteristic behavior, anatomical structure or physical system, such as immune response; metabolic response, or gene or gene variant to the members of one species. It refers to that property which differentiates one species from another but it is also used for phylogenetic levels higher or lower than the species.
Immunoglobulins
Multi-subunit proteins which function in IMMUNITY. They are produced by B LYMPHOCYTES from the IMMUNOGLOBULIN GENES. They are comprised of two heavy (IMMUNOGLOBULIN HEAVY CHAINS) and two light chains (IMMUNOGLOBULIN LIGHT CHAINS) with additional ancillary polypeptide chains depending on their isoforms. The variety of isoforms include monomeric or polymeric forms, and transmembrane forms (B-CELL ANTIGEN RECEPTORS) or secreted forms (ANTIBODIES). They are divided by the amino acid sequence of their heavy chains into five classes (IMMUNOGLOBULIN A; IMMUNOGLOBULIN D; IMMUNOGLOBULIN E; IMMUNOGLOBULIN G; IMMUNOGLOBULIN M) and various subclasses.
Farmer's Lung
A form of alveolitis or pneumonitis due to an acquired hypersensitivity to inhaled antigens associated with farm environment. Antigens in the farm dust are commonly from bacteria actinomycetes (SACCHAROPOLYSPORA and THERMOACTINOMYCES), fungi, and animal proteins in the soil, straw, crops, pelts, serum, and excreta.
Isoelectric Focusing
Carbohydrates
Serum Globulins
Agar
A complex sulfated polymer of galactose units, extracted from Gelidium cartilagineum, Gracilaria confervoides, and related red algae. It is used as a gel in the preparation of solid culture media for microorganisms, as a bulk laxative, in making emulsions, and as a supporting medium for immunodiffusion and immunoelectrophoresis.
Antibodies
Yaba monkey tumor virus
gamma-Globulins
Serum globulins that migrate to the gamma region (most positively charged) upon ELECTROPHORESIS. At one time, gamma-globulins came to be used as a synonym for immunoglobulins since most immunoglobulins are gamma globulins and conversely most gamma globulins are immunoglobulins. But since some immunoglobulins exhibit an alpha or beta electrophoretic mobility, that usage is in decline.
Blastomyces
Hepatitis B Antigens
Visna-maedi virus
A species of LENTIVIRUS, subgenus ovine-caprine lentiviruses (LENTIVIRUSES, OVINE-CAPRINE), that can cause chronic pneumonia (maedi), mastitis, arthritis, and encephalomyelitis (visna) in sheep. Maedi is a progressive pneumonia of sheep which is similar to but not the same as jaagsiekte (PULMONARY ADENOMATOSIS, OVINE). Visna is a demyelinating leukoencephalomyelitis of sheep which is similar to but not the same as SCRAPIE.
Actinobacillus
Chemical Precipitation
Isoelectric Point
Antigen-Antibody Complex
snRNP Core Proteins
Hemagglutination Inhibition Tests
Chromatography
Techniques used to separate mixtures of substances based on differences in the relative affinities of the substances for mobile and stationary phases. A mobile phase (fluid or gas) passes through a column containing a stationary phase of porous solid or liquid coated on a solid support. Usage is both analytical for small amounts and preparative for bulk amounts.
Sheep
Lentivirus Infections
Coccidioidin
Immunologic Techniques
Antibody Specificity
Chromatography, DEAE-Cellulose
Antitoxins
Neutralization Tests
The measurement of infection-blocking titer of ANTISERA by testing a series of dilutions for a given virus-antiserum interaction end-point, which is generally the dilution at which tissue cultures inoculated with the serum-virus mixtures demonstrate cytopathology (CPE) or the dilution at which 50% of test animals injected with serum-virus mixtures show infectivity (ID50) or die (LD50).
Colostrum
Electrophoresis
Streptococcus
Reagent Kits, Diagnostic
Brucella abortus
Complement C3
A glycoprotein that is central in both the classical and the alternative pathway of COMPLEMENT ACTIVATION. C3 can be cleaved into COMPLEMENT C3A and COMPLEMENT C3B, spontaneously at low level or by C3 CONVERTASE at high level. The smaller fragment C3a is an ANAPHYLATOXIN and mediator of local inflammatory process. The larger fragment C3b binds with C3 convertase to form C5 convertase.
Properdin
A 53-kDa protein that is a positive regulator of the alternate pathway of complement activation (COMPLEMENT ACTIVATION PATHWAY, ALTERNATIVE). It stabilizes the ALTERNATIVE PATHWAY C3 CONVERTASE (C3bBb) and protects it from rapid inactivation, thus facilitating the cascade of COMPLEMENT ACTIVATION and the formation of MEMBRANE ATTACK COMPLEX. Individuals with mutation in the PFC gene exhibit properdin deficiency and have a high susceptibility to infections.
Ultracentrifugation
Chromatography, Affinity
Antibodies, Antinuclear
Autoantibodies directed against various nuclear antigens including DNA, RNA, histones, acidic nuclear proteins, or complexes of these molecular elements. Antinuclear antibodies are found in systemic autoimmune diseases including systemic lupus erythematosus, Sjogren's syndrome, scleroderma, polymyositis, and mixed connective tissue disease.
Enterotoxins
Encephalitis Viruses
Plants, Edible
An organism of the vegetable kingdom suitable by nature for use as a food, especially by human beings. Not all parts of any given plant are edible but all parts of edible plants have been known to figure as raw or cooked food: leaves, roots, tubers, stems, seeds, buds, fruits, and flowers. The most commonly edible parts of plants are FRUIT, usually sweet, fleshy, and succulent. Most edible plants are commonly cultivated for their nutritional value and are referred to as VEGETABLES.
Bluetongue virus
Antibody Formation
Blood Proteins
Arthritis-Encephalitis Virus, Caprine
Swine
Any of various animals that constitute the family Suidae and comprise stout-bodied, short-legged omnivorous mammals with thick skin, usually covered with coarse bristles, a rather long mobile snout, and small tail. Included are the genera Babyrousa, Phacochoerus (wart hogs), and Sus, the latter containing the domestic pig (see SUS SCROFA).
The isolation and partial characterization of the serum lipoproteins and apolipoproteins of the rainbow trout. (1/3737)
1. VLD (very-low-density), LD (low-density) and HD (high-density) lipoproteins were isolated from the serum of trout (Salmo gairdneri Richardson). 2. Each lipoprotein class resembled that of the human in immunological reactivity, electrophoretic behaviour and appearance in the electron microscope. Trout LD lipoprotein, however, was of greater density than human LD lipoprotein. 3. The trout lipoproteins have lipid compositions which are similar to those of the corresponding human components, except for their high contents of long-chain unsaturated fatty acids. 4. HD and LD lipoproteins were immunologically non-identical, whereas LD lipoproteins possessed antigenic determinants in common with VLD lipoproteins. 5. VLD and HD lipoproteins each contained at least seven different apoproteins, whereas LD liprotein was composed largely of a single apoprotein which resembled human apolipoprotein B. 6. At least one, and possibly three, apoprotein of trout HD lipoprotein showed features which resemble human apoprotein A-1.7. The broad similarity between the trout and human lipoprotein systems suggests that both arose from common ancestral genes early in evolutionary history. (+info)Studies of the binding of different iron donors to human serum transferrin and isolation of iron-binding fragments from the N- and C-terminal regions of the protein. (2/3737)
1. Trypsin digestion of human serum transferrin partially saturated with iron(III)-nitrilotriacetate at pH 5.5 or pH 8.5 produces a carbohydrate-containing iron-binding fragment of mol.wt. 43000. 2. When iron(III) citrate, FeCl3, iron (III) ascorabate and (NH4)2SO4,FeSO4 are used as iron donors to saturate the protein partially, at pH8.5, proteolytic digestion yields a fragment of mol.wt. 36000 that lacks carbohydrate. 3. The two fragments differ in their antigenic structures, amino acid compositions and peptide 'maps'. 4. The fragment with mol.wt. 36000 was assigned to the N-terminal region of the protein and the other to the C-terminal region. 5. The distribution of iron in human serum transferrin partially saturated with various iron donors was examined by electrophoresis in urea/polyacrylamide gels and the two possible monoferric forms were unequivocally identified. 6. The site designated A on human serum transferrin [Harris (1977) Biochemistry 16, 560--564] was assigned to the C-terminal region of the protein and the B site to the N-terminal region. 7. The distribution of iron on transferrin in human plasma was determined. (+info)Mushroom worker's lung resulting from indoor cultivation of Pleurotus osteatus. (3/3737)
Indoor cultivation of oyster mushroom Pleurotus osteatus lead to an outbreak of extrinsic allergic alveolitis in two workers. High titer of indirect fluorescent antibody and positive precipitins against basidiospores of P. osteatus were demonstrated in sera of the patients. Mushroom workers should protect themselves from the basidiospores, being aware of their pathogenicity. (+info)Chemical and immunochemical measurement of total iron-binding capacity compared. (4/3737)
Radiometric, colorimetric, and two immunochemical methods for measuring total iron-binding capacity are compared. We evaluated the procedures on the basis of precision, applicability to a pediatric population, and accuracy as assessed by analytical recovery of purified transferrin. The immunoephelometric assay for transferrin provides significant advantages over the other methods examined. (+info)Purification of two dexamethasone-binding proteins from rat-liver cytosol. (5/3737)
Two dexamethasone-binding proteins have been purified from rat liver cytosol. The main purification steps are: precipitation by protamine sulphate, affinity chromatography on CH-Sepharose 4B to which 11-deoxycorticosterone is linked through a disulfide bond and DEAE-cellulose chromatography. Two binding components elute from the DEAE-cellulose column at 0.12 M and 0.2 M NaCl, respectively. By means of dodecylsulphate/polyacrylamide gel electrophoresis it was demonstrated that both components are composed predominantly of a single polypeptide with molecular weights of about 45 000 and 90 000. Antibodies to the two polypeptides have been elicited in rabbits. The antibodies to the 45 000-Mr polypeptide cross react with the 90 000-Mr component. Likewise the antibodies to the 90 000-Mr protein precipitate the 45 000-Mr polypeptide. Either of the two antibody preparations immunoprecipitates the major part (approximately 70%) of the dexamethasone-binding activity of the cytosol. (+info)Carbohydrate on human factor VIII/von Willebrand factor. Impairment of function by removal of specific galactose residues. (6/3737)
Human factor VIII/von Willebrand factor protein containing 120 +/- 12 nmol of sialic acid and 135 +/- 13 nmol of galactose/mg of protein was digested with neuraminidase. The affinity of native factor VIII/von Willebrand factor and its asialo form for the hepatic lectin that specifically binds asialoglycoproteins was assessed from in vitro binding experiments. Native factor VIII/von Willebrand factor exhibited negligible affinity while binding of the asialo derivative was comparable to that observed for asialo-alpha1-acid glycoprotein. Incubation of asialo-factor VIII/von Willebrand factor with Streptococcus pneumoniae beta-galactosidase removed only 62% of the galactose but abolished binding to the purified hepatic lectin. When the asialo derivative was incubated with purified beta-D-galactoside alpha2 leads to 6 sialyltransferase and CMP-[14C]NeuAc, only 61% of the galactose incorporated [14C]NeuAc. From the known specificites of these enzymes, it is concluded that galactose residues important in lectin binding are present in a terminal Gal/beta1 leads to 4GlcNAc sequence on asialo-factor VIII/von Willebrand factor. The relative ristocetin-induced platelet aggregating activity of native, asialo-, and agalacto-factor VIII/von Willebrand factor was 100:38:12, respectively, while procoagulant activity was 100:100:103. (+info)Immunological comparison of the proteins of chicken and rat liver ribosomes. (7/3737)
A comparison of the proteins of chicken and rat liver ribosomes using immunochemical techniques was undertaken. The procedures included quantitative precipitation, passive hemagglutination, and immunodiffusion on Ouchterlony plates. The results indicate that antisera specific for chicken or rat liver ribosomes recognize only about 20% of common determinants. While there are important reservations, the results suggest extensive differences in the proteins of rat and chicken liver ribosomes. Despite those differences, rat and chicken liver ribosomal proteins maintain some homologous sequences present in bacterial ribosomal proteins. An enriched antibody preparation against chicken 80 S ribosomes inhibited the poly(U)-directed synthesis of polyphenylalanine and the elongation factor G (EF-G)-catalyzed binding of [3H]GDP to Escherichia coli ribosomes. Thus, chicken liver ribosomes, like ribosomes from rat liver and yeast, must have proteins homologous with those of E. coli ribosomes. (+info)Homogeneous pyruvate kinase isolated from yeast by two different methods is indistinguishable from pyruvate kinase in cell-free extract. (8/3737)
In this report, we have compared homogeneous yeast (Saccharomyces cerevisiae) pyruvate kinase to enzyme from cell-free extracts in several different ways: 1) isoelectric focusing of cell-free extracts indicates one peak of pyruvate kinase activity whose isoelectric point is the same as that of the pure enzyme; 2) antibody prepared to the pure enzyme produces a single, fused precipitin line against enzyme in the cell-free extract and pure enzyme; 3) immunoelectrophoresis of cell-free extract produces one precipitin arc which has the same mobility as that of the pure enzyme; and 4) immunoprecipitation of the pure enzyme from cell-free extract with subsequent solubilization in 1% sodium dodecyl sulfate and electrophoresis on sodium dodecyl sulfate-polyacrylamide gels produces a single protein band attributable to pyruvate kinase which co-migrates with the purified enzyme. Within the limits of the sensitivity of the methods employed, we conclude that the homogeneous pyruvate kinase prepared from yeast lysed either by Manton-Gaulin homogenization (Aust, A., Yun, S.-L., and Suelter, C. (1975) Methods Enzymol. 42, 176-182) or by toluolysis (Yun, S.-L., Aust, A.E., and Suelter, C.H. (1977) J. Biol. Chem. 251, 124-128) is identical with pyruvate kinase in cell-free extract. (+info)
Silver-enhanced radial immunodiffusion assay of plasma apolipoprotein by B Y. Ishida and B Paigen
Antigen-Antibody Interactions
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A cohort study of the association between serum immunoglobulin G concentration and preweaning health, growth, and survival in...
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Immunodiffusion
Photograph of precipitin circles in a Petri dish during radial immunodiffusion. "Diffusion Patterns". Immunodiffusion ... Ouchterlony double immunodiffusion) Coleman, R. M.; Kaufman, L. (1972). "Use of the Immunodiffusion Test in the Serodiagnosis ... Immunodiffusion is a diagnostic test which involves diffusion through a substance such as agar which is generally soft gel agar ... Immunodiffusion at the US National Library of Medicine Medical Subject Headings (MeSH) v t e (Biological techniques and tools, ...
Radial immunodiffusion
... (RID) or Mancini method, Mancini immunodiffusion or single radial immunodiffusion assay, is an ... Stanley J (2002). "Chapter 12: Precipitation: Single Radial Immunodiffusion: Laboratory Technique 12-1: Radial Immunodiffusion ... Introductory lecture/slideshow illustrating radial immunodiffusion theory and technique. (6:56 minutes) "Radial Immunodiffusion ... Introductory video demonstrating radial immunodiffusion technique (3:43 minutes). "Radial Immunodiffusion (Mancini Technique ...
Ouchterlony double immunodiffusion
... (also known as passive double immunodiffusion) is an immunological technique used in the ... Photographs of Ouchterlony immunodiffusion patterns showing stained precipitin lines of full identity, partial identity and non ... "Diffusion Patterns". Immunodiffusion principles and application. Archived from the original on 2019-12-11. Retrieved 2017-05-19 ... 2008). "Immunodiffusion and the Ouchterlony Method". Fundamental Immunology (6th ed.). Lippincott Williams & Wilkins. pp. 176- ...
Elek's test
The test uses immunodiffusion. A strip of filter paper impregnated with diphtheria antitoxin is buried just beneath the surface ...
Mycobacterium flavescens
Serologic specificity demonstrated by immunodiffusion. Related to Mycobacterium fortuitum: can be distinguished by its intense ...
Vespula
The findings were confirmed by immunodiffusion. Lines of identity were observed when antigen 5s, or phospholipases, from the ...
Antigen-antibody interaction
Adams, Trudy; Osborn, Sancha; Rijpkema, Sjoerd (2005). "An immuno-diffusion assay to assess the protective antigen content of ... Lietze, Arthur (1969). "Quantitation of Food Adulterants by Multiple Radial Immunodiffusion. I. Cross-Reacting Antigen Mixtures ... Hornbeck, P. (2001). "Double-immunodiffusion assay for detecting specific antibodies". Current Protocols in Immunology. Chapter ...
Eumycetoma
Other tests include ELISA, immunodiffusion, and PCR with DNA sequencing (so-called DNA barcoding). X rays and ultrasonography ... Other tests include ELISA, immunodiffusion, and DNA Barcoding. Treatment includes surgical removal of affected tissue and ...
Tam David-West
David-West, Tam S (1966). "The standardisation of some immunodiffusion systems in agar gel". Journal of Pathology and ...
Avian adenovirus
In addition, virus antigen can be detected double immunodiffusion. Postmortem examination may reveal a variety of clinical ...
Mycobacterium gilvum
Differential characteristics 5 species-specific antigens, demonstrable in immunodiffusion tests. Not assumed to be pathogenic. ...
Chronic pulmonary aspergillosis
Coleman RM, Kaufman L (February 1972). "Use of the immunodiffusion test in the serodiagnosis of aspergillosis". Applied ...
Immunoelectrophoresis
Radial immunodiffusion The radial immunodiffusion is an immunoassay technique for determining the concentration of a particular ... Immuno-Diffusion (Articles with short description, Short description matches Wikidata, All articles with unsourced statements, ... The main two principles of immunoelectrophoresis are zone electrophoresis and immunodiffusion. Agarose as 1% gel slabs of about ...
Mycobacterium farcinogenes
Ridell, M (March 1983). "Immunodiffusion analyses of Mycobacterium farcinogenes, Mycobacterium senegalense and some other ...
Extractable nuclear antigen
Double immunodiffusion (DID) and counterimmunoelectrophoresis (CIEP), two forms of gel-based techniques, are used to gain ... "Abbexa - Antibodies, Proteins, ELISA kits". Bunn, C.; Kveder, T. (1996). "Counterimmunoelectrophoresis and immunodiffusion for ... double immunodiffusion (DID) and counterimmunoelectrophoresis (CIEP). Both of these techniques require the precipitation of ... which are screened for by Ouchterlony double immuno diffusion techniques and confirmed by immunoblotting. On anti-nuclear ...
Virus quantification
Single radial immunodiffusion assay (SRID), also known as the Mancini method, is a protein assay that detects the amount of ... specific viral antigen by immunodiffusion in a semi-solid medium (e.g. agar). The medium contains antiserum specific to the ... ". "Pierce Protein Biology". Rodda, S.J.; Gallichio, H.A.; Hampson, A.W (1981). "The Single Radial Immunodiffusion Assay ...
Fasciola hepatica
Using ELISA is more specific than using a Western blot or Arc2 immunodiffusion. Proteases secreted by F. hepatica have been ...
Alpha-1 antitrypsin
Other detection methods include the use of enzyme-linked-immuno-sorbent-assays and radial immunodiffusion. Different analytical ...
Cymbidium mosaic virus
The virus can be detected with ELISA, immunodiffusion tests, or a nucleic acid hybridization assay. The Cymbidium mosaic virus ...
Paracoccidioidomycosis
Gel immunodiffusion is commonly used in endemic areas, diagnoses 95% of cases with high specificity. Complement fixation allows ...
Tree nut allergy
Goetz, DW (July 2005). "Cross-reactivity among edible nuts: double immunodiffusion, crossed immunoelectrophoresis, and human ... double immunodiffusion, crossed immunoelectrophoresis, and human specific IgE serologic surveys". Annals of Allergy, Asthma & ...
Mycobacterium duvalii
Differential characteristics Characterised by the possession of 6 species-specific antigens demonstrable in immunodiffusion ...
Bovine leukemia virus
Diagnosis relies on agar gel immunodiffusion, ELISA (enzyme linked immunosorbent assay) and PCR (polymerase chain reaction). ...
Bovine foamy virus
Malmquist, W. A.; Van der Maaten, M. J.; Boothe, A. D. (January 1969). "Isolation, immunodiffusion, immunofluorescence, and ...
Avian orthoreovirus
They can be confirmed using virus isolation, complement fixation, ELISA, immunodiffusion or histopathology (following ... agar gel immunodiffusion, indirect immunofluorescence (IIF), and virus neutralization (VN). Virus neutralization identifies ...
Horse racing in the Philippines
The tests adopted were the Coggins Test (also known as the Agar Gel Immuno-Diffusion (AGID) Test). In 1995, the Blood Typing ...
Alpha-1 antitrypsin deficiency
Other detection methods include use of enzyme-linked-immuno-sorbent-assays in vitro and radial immunodiffusion. Alpha-1 ...
Ferae
Research based on immunodiffusion technique and comparison of protein and DNA sequences revealed the close relationships ...
Capripoxvirus
... immunofluorescence test or agar gel immunodiffusion test. They, however, can be isolated by using the HinDill restriction ...
Auchterlonie
Ouchterlony O (1970), Handbook of Immunodiffusion and Immunoelectrophoresis, Ann Arbor, MI: Ann Arbor-Humphrey Science ... was a Swedish bacteriologist and immunologist who is credited with the creation of the Ouchterlony double immuno diffusion test ...
Browsing by Subject "Immunodiffusion"
A collaborative study on the use of single radial immunodiffusion for the assay of rabies virus glycoprotein / by M. Ferguson,...
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histoplasma immunodiffusion tests
MiraVista Diagnostics offers histoplasma immunodiffusion tests. Contact us to order tests today. ... Contact us to learn more about histoplasma immunodiffusion tests.. Share Share Email Print ... MiraVista Diagnostics Added Histoplasma Immunodiffusion Tests to its Fungal Test Menu. MiraVista Diagnostics has added ... histoplasma immunodiffusion tests to its menu of fungal infection diagnostic antibody detection tests. This assay aids in the ...
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Comparison of electrophoresis, isoelectric focusing, and immunodiffusion in determinations of cow's and goat's milk in Serra da...
Une étude comparative de trois méthodes - électrophorèse (PAGE), isoélectrotocalisation (IEF) et immunodiffusion radiale (RID ... Key words: PAGE and IEF whey proteins / cows and goats milk detection / immunodiffusion / Serra da Estrela cheese Mots clés ... Comparison of electrophoresis, isoelectric focusing, and immunodiffusion in determinations of cows and goats milk in Serra da ... Abstract - A comparative study of electrophoresis (PAGE), isoelectric focusing (IEF), and radial immunodiffusion (RID) in ...
096719: Candida Antibodies, Qualitative | Labcorp
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MESH TREE NUMBER CHANGES - 2008 MeSH
Adenovirus Antibody (1E11) (NB200-425): Novus Biologicals
Neurologic Manifestations in Primary Sjögren Syndrome: A Stu... : Medicine
The presence of anti-Ro/SSA, anti-La/SSB, and ENA antibodies was evaluated by Ouchterlony double gel immunodiffusion (all ... Screening for autoantibodies to Ro/SSA and La/SSB was systematically performed by Ouchterlony double gel immunodiffusion and by ... Immunoblotting was more sensitive (46%) than Ouchterlony immunodiffusion (26%). Anti-Ro/SSA antibodies were not more frequent ... positive detection of anti-Ro/SSA by Ouchterlony immunodiffusion; SSA WB, Ouc-, positive detection of anti-Ro/SSA by ...
anti-Complement C4 Antikörper (AA 1450-1723) | Produkt Nr. ABIN6995381
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Plasma IgG concentrations were determined by radial immunodiffusion assay. Neutrophil function was assessed by a flow cytometry ... Plasma IgG concentrations were determined by radial immunodiffusion assay. Neutrophil function was assessed by a flow cytometry ... Plasma IgG concentrations were determined by radial immunodiffusion assay. Neutrophil function was assessed by a flow cytometry ...
Development of an Immunochromatographic Test Strip for Detection of Cholera Toxin
LOINC 15437-7 - Avian encephalomyelitis virus Ab [Presence] in Serum by Immune diffusion (ID)
Versiti - Diagnostic Laboratories | Versiti
Case Definitions for Infectious Conditions Under Public Health Surveillance
Herd Health of Llamas and Alpacas - Exotic and Laboratory Animals - Merck Veterinary Manual
RadialDouble immunodiffusionAgar-gel immunodiffusion testAntibodiesELISAAGIDAntibodyRadialeImmunoblotHistoplasmaSerumComplementCompositionSerumAssayAntigenDetermined by radial immunodiffusionElectrophoresisRadial immunodiffusion methodAntibodiesAntigensComplementSerologicalPrecipitin linesImmunoelectrophoresisSRIDTestDoubleSingleMethodRESULTSPositiveHumanSamplesResultVirusMassTechniques
Radial5
- Radial Immunodiffusion is a technique used extensively for the quantitative estimation of antigens. (synbiosis.com)
- IMSEAR at SEARO: Immunoglobulin estimation in cerebrospinal fluid by modified radial immunodiffusion. (who.int)
- Abstract - A comparative study of electrophoresis (PAGE), isoelectric focusing (IEF), and radial immunodiffusion (RID) in determinations of cow's and goat's milks in Serra da Estrela cheeses was carried out. (dairy-journal.org)
- Plasma IgG concentrations were determined by radial immunodiffusion assay. (avma.org)
- Moreover, other associated tests include Protein-C antigenic assay (which is not based on function or activity and helps to distinguish between type-1 and type-2 PC abnormalities) is performed by ELISA, radial immunodiffusion - RID etc. (rtdiagnostics.net)
Double immunodiffusion2
- In all the patients, the diagnosis of systemic PCM was made by the demonstration of the P. brasiliensis organisms or positive serology, DID (double immunodiffusion). (nih.gov)
- Antibodies were tested for specificity using double-immunodiffusion assays in agarose. (antibodiesinc.com)
Agar-gel immunodiffusion test2
- EIA was difficult to diagnose and identify until 1970, when the agar-gel immunodiffusion test, commonly known as the Coggins test, was developed. (okstate.edu)
- Serum samples (n = 533) were collected from adult male and female goats for detection of antibodies against CAEV by agar gel immunodiffusion test. (edu.pe)
Antibodies2
- Immunoblot reactivity as well as die presence of precipitating canine antibodies, determined by Ouchterlony immunodiffusion (ID), was found to be strictly associated with one of the DF ANA subtypes. (slu.se)
- Detection of antibodies to Blastomyces, Coccidioides, and Histoplasma by agar gel immunodiffusion (AGID). (tamu.edu)
ELISA1
- Agar gel immunodiffusion (AGID), enzyme-linked immunosorbent assay (ELISA), and polymerase chain reaction (PCR) tests are all used to identify BLV-positive animals. (usda.gov)
AGID1
- Agar Gel Immunodiffusion (AGID) test: This test, which identifies a certain protein on the Brucella organism, is considered the most accurate test. (embracepetinsurance.com)
Antibody2
- MiraVista Diagnostics has added histoplasma immunodiffusion tests to its menu of fungal infection diagnostic antibody detection tests. (miravistalabs.com)
- The Coccidioides Antibody, Complement Fixation and Immunodiffusion test contains 1 test with 2 biomarkers. (ultalabtests.com)
Radiale1
- inflammatoires et nutritionnelles ont ete dosees par immunodiffusion radiale de Mancini. (bvsalud.org)
Immunoblot1
- Immunodiffusion and immunoblot procedures showed that these strains were identical. (bmj.com)
Histoplasma2
- Contact us to learn more about histoplasma immunodiffusion tests. (miravistalabs.com)
- Histoplasma H and M bands were demonstrated in blood by immunodiffusion (ID) conducted by the National Center for Mycology (Edmonton), and culture of sputum and lung biopsy material yielded Histoplasma capsulatum. (cdc.gov)
Serum1
- A study was designed to determine whether quantitative determinations could be made of organophosphate exposure by determining the ratio of serum cholinesterase activity to the amount of total butyrylcholinesterase (BCHE) protein determined by immunodiffusion. (cdc.gov)
Complement1
- Immunodiffusion and complement fixation (CF) methods can detect coccidioidal immunoglobulin G (IgG). (medscape.com)
Composition1
- Morphology and Composition of Immunodiffusion Precipitin Complexes Evaluated via Microscopy and Proteomics. (bvsalud.org)
Serum7
- METHODS: Serum samples stored at -20 degrees C from 50 patients with pigeon breeder's disease, 50 control samples from patients with other respiratory diseases, and 50 healthy blood donors were examined for the precipitating antibodies and IgG antibodies to antigens present in extract of pigeon droppings by immunodiffusion and enzyme linked immunosorbent assay (ELISA), respectively. (bmj.com)
- We created dichotomous variables to indicate whether positive test results for histoplasmosis were obtained by enzyme immunoassay (of serum, urine, or another or unspecified specimen type), immunodiffusion, complement fixation, PCR, culture, microscopy, or other or unspecified histoplasmosis test. (medscape.com)
- A study was designed to determine whether quantitative determinations could be made of organophosphate exposure by determining the ratio of serum cholinesterase activity to the amount of total butyrylcholinesterase (BCHE) protein determined by immunodiffusion. (cdc.gov)
- A radial immunodiffusion technique was used to measure LMW-C1q in the serum of 54 patients. (jimmunol.org)
- 10 g/L. Immunoglobulin G can be measured directly in calf serum using radial immunodiffusion (RID), or indirectly estimated by measuring total protein (TP). (gla.ac.uk)
- ProtoCOL 3 is the next generation instrument for colony counting, zone measurements (inhibition and antibiotic susceptibility testing), membranes, PETRIFILM TM plates and a range of other applications including: spiral plates, OPKA (opsonophagocytic killing assay), SBA (serum bactericidal assay), Multi-sector, Multi-well, SRD (single radial immunodiffusion) and Ames. (emphordlas.com)
- Determination of immunoglobulin in the serum was performed by radial immunodiffusion Mancini G. (facemsport.ro)
Assay4
- Comparison of immunodiffusion and enzyme linked immunosorbent assay in the detection of abnormal antibodies in pigeon breeder's disease. (bmj.com)
- Abstract The single radial immunodiffusion assay has been the accepted method for determining the potency of inactivated influenza vaccines since 1978. (bionity.com)
- Radial immunodiffusion assay belongs to the precipitation method. (uniba.sk)
- This quantitative assay is based on immunodiffusion of antigens and antibodies in the gel and can be used in level analysis of complement components (C3, C4), inflammation proteins (CRP) and immunoglobulins (IgG, IgM and IgA). (uniba.sk)
Antigen2
- The Ouchterlony double-immunodiffusion showed that the antigen prepared from the tilapia strain possessed surface antigens similar to A. hydrophila type strain and the strains isolated from pacu. (usp.br)
- This paper describes the incidence of Au/SH antigen as determined by the immunodiffusion technic in 206 patients with a variety of diseases, and hospital personnel at a university medical center. (sma.org)
Determined by radial immunodiffusion1
- C6 and Factor B were determined by radial immunodiffusion. (edu.au)
Electrophoresis3
- The final product is then subjected to rigorous quality control assays including immunodiffusion, solid-phase enzyme immunoassays, gel electrophoresis and solid-phase binding assays. (vectorlabs.com)
- Peak A, showed antigenic cross reactivity with rabbit anti-(Torpedo membrane) antiserum in immunodiffusion and electrophoresis assays and inhibited the precipitation of 125I-labelled purified Torpedo acetylcholine receptor by rabbit anti-AChR IgG. (bath.ac.uk)
- Separation of nucleic acids and proteins by electrophoresis, chromatography or immunodiffusion. (hispanagar.com)
Radial immunodiffusion method2
- IgG1 concentrations were tested using a radial immunodiffusion method. (inrae.fr)
- C3 activator, C1 esterase inhibitor (C1-INH), IgA, IgG and IgM were estimated by the single radial immunodiffusion method. (smj.org.sg)
Antibodies2
Antigens4
- The 13 sera from horses with proven active pythiosis were positive in immunodiffusion tests with the culture filtrate antigens. (nih.gov)
- Single radial immunodiffusion (RID) is used extensively for the quantitative estimation of antigens. (androbose.in)
- 20. Detection of tumor-specific antigens in human mucinous cystadenocarcinoma of the ovary by immunodiffusion. (nih.gov)
- Immunodiffusion also indicates identity, cross reaction and non identify between different antigens. (alltro.in)
Complement1
- Measurement of complement components in systemic lupus erythematosus by radial immunodiffusion. (umassmed.edu)
Serological1
- The analysis of PCM was confirmed by direct examination of biological fluids and/or serological immunodiffusion checks. (saracatinib.info)
Precipitin lines1
- Identification of caprine arthritis-encephalitis retrovirus proteins in immunodiffusion precipitin lines. (microbiologyresearch.org)
Immunoelectrophoresis3
- The different elapid venoms, with the exception of that of N. naja oxiana , showed a considerable number of identical and similar precipitin components by immunodiffusion and immunoelectrophoresis. (ajtmh.org)
- In immunoelectrophoresis and radial immunodiffusion (Ouchterlony), using various antiserum concentrations against fresh normal swine plasma a single precipitin line is obtained which shows a reaction of identity with the precipitin line obtained with purified alpha-1antitrypsin. (lsbio.com)
- In precipitating techniques as immunoelectrophoresis and single or double radial immunodiffusion to identify the presence of alpha-1antitrypsin in swine plasma or other body fluids or to determine its concentration. (lsbio.com)
SRID1
- The routinely used method to quantitate the haemagglutinin (HA) content in the influenza vaccines is Single Radial Immunodiffusion (SRID). (nih.gov)
Test6
- A practical, sensitive, and specific immunodiffusion test was developed for diagnosing and monitoring pythiosis in horses. (nih.gov)
- Immunodiffusion test for diagnosing human pythiosis. (nih.gov)
- Immunodiffusion test results indicating H band, M band, or both were considered positive. (medscape.com)
- Four case-patients had B. ranarum cultured from surgical specimens, and four had a positive serologic result using an immunodiffusion test at CDC (1). (cdc.gov)
- Double immunodiffusion test suggested that P158 has high antigenicity homology with BBWV2 isolate B935. (zju.edu.cn)
- The Elek test is an immunodiffusion test that tests for the toxigenicity of the toxin. (xs4all.nl)
Double3
- When analyzed immunochemically by double immunodiffusion, this low m.w. (jimmunol.org)
- AACE was found to be superior for this to double immunodiffusion. (gla.ac.uk)
- Specific immune response Ag-Ab was detected by double immunodiffusion in agar gel in 100% of rabbits using protocols I and II and 60% with protocol III in large macrocysts while 100, 60 and 40% response in protocols I, II and III in small macrocysts respectively. (edu.pe)
Single1
- Single radial immunodiffusion (SRD) is a simple yet powerful technique that is routinely used in many clinical laboratories for a wide variety of analyses. (synbiosis.com)
Method1
- It is shown that at the stage of acceptance of raw materials the method of identification of meat on the basis of immunodiffusion is effective and specific, it is more economic than methods on the basis of PCR-diagnostics. (rudn.ru)
RESULTS1
- there was no direct correlation with the immunodiffusion results. (ajtmh.org)
Positive1
- Serology by immunodiffusion is positive in more than 80% of cases. (mhmedical.com)
Human1
- Immunodiffusion studies in human schistosomiasis mansoni. (usp.br)
Samples1
- Samples seropositive by EIA underwent confirmatory immunodiffusion testing at the University of California Davis Coccidioidomycosis Reference Laboratory (Davis, CA, USA). (cdc.gov)
Result1
- We defined seroconversion as IgG or IgM detected in a posttransfer sample by EIA, confirmed by immunodiffusion if performed, with a seronegative pretransfer EIA result. (cdc.gov)
Virus1
- The virus was found by immunodiffusion analysis to be serologically related to several definitive tombusvirus species. (sipav.org)
Mass1
- Total 1.25(OH)2D was measured by mass spectrometry and vitamin D binding protein (DBP) by immunodiffusion. (eurekalert.org)
Techniques1
- ab229797 reacted only with anti IgE and not with anti IgG, IgA, IgM or IgD by immunodiffusion and IEP techniques. (abcam.com)