Immunoassay
Immunoenzyme Techniques
Fluorescence Polarization Immunoassay
Enzyme Multiplied Immunoassay Technique
Reagent Kits, Diagnostic
Sensitivity and Specificity
Enzyme-Linked Immunosorbent Assay
Fluoroimmunoassay
Radioimmunoassay
Cross Reactions
False Positive Reactions
Evaluation Studies as Topic
Luminescent Measurements
Substance Abuse Detection
Immunoglobulin G
Nephelometry and Turbidimetry
Europium
Reproducibility of Results
Antibody Specificity
Gas Chromatography-Mass Spectrometry
Immunoglobulin M
False Negative Reactions
Antibodies, Immobilized
Latex Fixation Tests
Digoxin
Quality Control
Reference Standards
Feces
Calibration
Indicators and Reagents
Fluorescent Antibody Technique
Antibodies
Clinical Laboratory Techniques
Microspheres
Reference Values
Biological Markers
Microchemistry
Chromatography, High Pressure Liquid
Phenytoin
Limit of Detection
Antibodies, Heterophile
Fluorescence Polarization
alpha-Fetoproteins
Immunoradiometric Assay
Radioimmunoprecipitation Assay
Predictive Value of Tests
Immune Sera
Rubella virus
Latex
Immunologic Techniques
Streptavidin
Rotavirus
Thyroxine
Seroepidemiologic Studies
Saliva
Polymerase Chain Reaction
Horseradish Peroxidase
Specimen Handling
Pregnancy
Fluorescent Antibody Technique, Direct
Serum
Biological Assay
Gastroenteritis
Rabbits
Chorionic Gonadotropin, beta Subunit, Human
Avidin
Blood Specimen Collection
Colorimetry
Clostridium difficile
Luminescence
Antibody Affinity
Biotin
Lanthanoid Series Elements
Antigen-Antibody Complex
Rubella
Microfluidic Analytical Techniques
Virology
Rotavirus Infections
Autoantibodies
Thyrotropin
Biosensing Techniques
Chorionic Gonadotropin
Fluorometry
Hepatitis A Antigens
Point-of-Care Systems
Binding, Competitive
Agglutination Tests
Opiate Alkaloids
Immunoglobulin A
Rheumatoid Factor
Barbiturates
Immunoblotting
Cardenolides
Blotting, Western
Hepatitis Antibodies
Theophylline
Reagent Strips
Digoxigenin
Narcotics
Street Drugs
Nordazepam
Complement Fixation Tests
Molecular Sequence Data
Tandem Mass Spectrometry
Amphetamines
Hemagglutination Tests
Cattle
Polystyrenes
Enterocolitis, Pseudomembranous
Oxymorphone
Carcinoembryonic Antigen
Antigen-Antibody Reactions
Protein Array Analysis
Galactosidases
Hepatitis B Surface Antigens
Pregnanediol
Automation
Alkaline Phosphatase
Microfluidics
Gentamicins
Case-Control Studies
Thyroxine-Binding Proteins
Dimethylpolysiloxanes
Acecainide
Urinalysis
Peptide Fragments
Mamastrovirus
Nasopharynx
Drug Monitoring
Giardiasis
Chemistry, Clinical
Mass Spectrometry
Immunodiffusion
HIV Antigens
beta-Galactosidase
Immunological comparison of the proteins of chicken and rat liver ribosomes. (1/4669)
A comparison of the proteins of chicken and rat liver ribosomes using immunochemical techniques was undertaken. The procedures included quantitative precipitation, passive hemagglutination, and immunodiffusion on Ouchterlony plates. The results indicate that antisera specific for chicken or rat liver ribosomes recognize only about 20% of common determinants. While there are important reservations, the results suggest extensive differences in the proteins of rat and chicken liver ribosomes. Despite those differences, rat and chicken liver ribosomal proteins maintain some homologous sequences present in bacterial ribosomal proteins. An enriched antibody preparation against chicken 80 S ribosomes inhibited the poly(U)-directed synthesis of polyphenylalanine and the elongation factor G (EF-G)-catalyzed binding of [3H]GDP to Escherichia coli ribosomes. Thus, chicken liver ribosomes, like ribosomes from rat liver and yeast, must have proteins homologous with those of E. coli ribosomes. (+info)Performance and specificity of monoclonal immunoassays for cyclosporine monitoring: how specific is specific? (2/4669)
BACKGROUND: Immunoassays designed for the selective measurement of cyclosporin A (CsA) inadvertently show cross-reactivity for CsA metabolites. The extent and clinical significance of the resulting overestimation is controversial. A comprehensive assessment of old and new methods in clinical specimens is needed. METHODS: In a comprehensive evaluation, CsA was analyzed in 145 samples with the new CEDIA assay and compared with the Emit assay with the old and new pretreatments, the TDx monoclonal and polyclonal assays, the AxSYM, and HPLC. All samples were from patients with liver and/or kidney transplants. RESULTS: The CEDIA offered the easiest handling, followed by the AxSYM, which showed the longest calibration stability. The TDx monoclonal assay provided the lowest detection limit and the lowest CVs. The mean differences compared with HPLC were as follows: Emit, 9-12%; CEDIA, 18%; AxSYM, 29%; and TDx monoclonal, 57%. The CycloTrac RIA paralleled the Emit results. In contrast to the mean differences, substantial (>200%) and variable overestimations of the CsA concentration were observed in individual patient samples. Metabolic ratios, estimates of the overall concentrations of several cross-reacting metabolites (nonspecific TDx polyclonal/specific reference method), correlated with the apparent biases of the various monoclonal assays. Metabolic ratios varied up to 10-fold, which translated into biases for individual samples between -7% and +174%. The higher the cross-reactivity of an assay was, the higher was the range of biases observed. The interindividual differences markedly exceeded other factors of influence (organ transplanted, hepatic function). CONCLUSION: Because assay bias cannot be predicted in individual samples, substantially erratic CsA dosing can result. The specificity of CsA assays for parent CsA remains a major concern. (+info)Automated homogeneous immunoassay for gentamicin on the dimension clinical chemistry system. (3/4669)
BACKGROUND: Monitoring of the concentration of gentamicin in serum and plasma during therapy is widely recommended and practiced in hospitals. Our aim was to develop a homogeneous immunoassay based on particle-enhanced turbidimetric inhibition immunoassay technology to quantify gentamicin on the Dimension clinical chemistry system. METHODS: Assay performance was assessed on each of the Dimension models in a 15-instrument interlaboratory comparison study. A split-sample comparison (n = 1171) was also performed between the gentamicin methods on the Dimension system and the Abbott TDx analyzer, using multiple reagent and calibrator lots on multiple instruments. RESULTS: The Dimension method was linear to 25.1 micromol/L (12.0 microg/mL) with a detection limit of 0.63 micromol/L (0.3 microg/mL). Calibration was stable for 30 days. The within-run imprecision (CV) was <1.3%, and total imprecision ranged from 1.8% to 3.2% between 4.2 micromol/L (2.0 microg/mL) and 16.7 micromol/L (8.0 microg/mL) gentamicin. Linear regression analysis of the results on the Dimension method (DM) vs the Abbott TDx yielded the following equation: DM = 0.98TDx - 0.42; r = 0.987. Minimal interference was observed from structurally related compounds such as sagamicin, netilmicin, and sisomicin. CONCLUSION: The monoclonal antibody used in this method has similar reactivities toward the individual gentamicin subspecies C1, C1a, and C2, thus providing analytical recovery not significantly dependent on relative subspecies concentrations. (+info)Serum IGF-binding protein-6 and prostate specific antigen in breast cancer. (4/4669)
OBJECTIVE: Recent studies have demonstrated the presence of the IGF-binding proteins (IGFBPs) and prostate specific antigen (PSA), an IGFBP protease. in human breast tissue. We sought to investigate the differences in serum IGFs, IGFBP-1, -3 and -6, and PSA between patients with surgically proven breast cancer and patients with benign breast disease. DESIGN AND METHODS: Concentrations of IGFs, IGFBP-1, -3 and -6, and PSA were determined in the sera from 57 patients with breast cancer (CA), and 46 women with benign breast disease (BBD) using immunoassays for IGFs and IGFBPs and an ultrasensitive ELISA for PSA. RESULTS: The mean (+/- S.E.M.) serum IGFBP-6 level in the CA group, 127 (16) ng/ml, was statistically significantly lower than in the BBD group, 157 (10) ng/ml (P = 0.016). Patients with CA had an elevated geometric mean serum PSA level of 0.018 (range: 0.0015-0.107) ng/ml, compared with 0.007 (range: 0.0015-0.019) ng/ml in women with BBD (P = 0.025). Mean serum IGFBP-1 concentrations were significantly lower in the CA group, 16 (2) ng/ml, versus 37 (4) ng/ml in the BBD group (P = 0.001). Mean serum IGFBP-3 concentrations were also lower in the CA group versus the BBD group, at 1981 (65) ng/ml, versus 2603 (140) ng/ml (P = 0.002) respectively. In the CA group, statistically significant correlations between PSA and IGFBP-6 (r = 0.413; P = 0.001), and between PSA and IGFBP-1 (r = -0.329; P = 0.021) were seen. Differences in IGF-I and -II between the two groups were not statistically significant. CONCLUSION: Lower serum concentrations of IGFBP-6, -3 and -1, but higher PSA concentrations were seen in the breast cancer group, and collectively these would suggest that there is an increase in bioavailable IGF-I in breast cancer. (+info)Differential immunodiagnosis between cystic hydatid disease and other cross-reactive pathologies. (5/4669)
We assessed an Echinococcus granulosus hydatid fluid antigen-ELISA (EgHF-ELISA) as a serologic prescreening test for E. granulosus infections, supplemented by more specific confirmatory tests, including arc-5 immunoprecipitation and antigen B subunit 8-kD immunoblotting. The diagnostic sensitivity of the EgHF-ELISA was 91%. With regard to the test specificity of the EgHF-ELISA (overall = 82%), we observed relatively frequent cross-reactions in tumor patients (6%) and in patients with other parasitic diseases. Cestode-related cross-reactivity can be resolved by the complementary use of E. multilocularis-specific antigens or Taenia solium cysticercosis-specific immunoblotting. Immunoblotting based upon the detection of antibody reactivity to the 8-kD antigen of EgHF, or if appropriately detectable, to the 29-kD and 34-kD bands exhibited a 91% diagnostic sensitivity and an overall specificity of 97% or 94%, respectively. Thus, immunoblotting provided a 99% discrimination between seropositive pre-operative cystic hydatid disease cases and cross-reactive non-cestode parasitic infections or malignancies. (+info)An immunoluminometric assay for N-terminal pro-brain natriuretic peptide: development of a test for left ventricular dysfunction. (6/4669)
Measurement of plasma levels of brain natriuretic peptide (BNP) has been used to assess left ventricular dysfunction and prognosis. Levels of the N-terminus of the precursor of BNP (NT-proBNP) have been reported to be elevated to a greater extent than BNP in left ventricular dysfunction. We have devised a non-radioactive sensitive and specific assay for NT-proBNP based on a competitive ligand binding principle. The chemiluminescent label 4-(2-succinimidyloxycarbonylethyl)phenyl-10-methylacridinium 9-carboxylate fluorosulphonate was used to label peptides representing domains in the middle and C-terminal sections of NT-proBNP. Assay of the C-terminal section of NT-proBNP (amino acids 65-76) in patients with proven left ventricular dysfunction [left ventricular wall motion index median 0.9 (range 0.3-1.4)] revealed elevated values [median 639 (386-911) fmol/ml] compared with normal controls [left ventricular wall motion index of 2 in all, NT-proBNP median 159 (120-245) fmol/ml, P<0.001]. Measurement of the middle section of NT-proBNP (amino acids 37-49) was not a discriminating test. It is thus possible to derivatize small peptides with a methyl acridinium label and preserve immunodetection with specific antibodies. Such methodology may allow non-radioactive immunoluminometric assays to be devised. (+info)Endometrial evaluation is not predictive for in vitro fertilization treatment. (7/4669)
PURPOSE: The main purpose of this study was to evaluate ovarian function by clomiphene citrate (CC) challenge test in a group of tubal infertile women and to study endometrial morphological maturation in the early luteal phase of CC-stimulated cycles as compared to in vitro fertilization (IVF) treatment outcome. METHODS AND RESULTS: Four women presented with strongly retarded, proliferative endometrium in the luteal phase. Of these, three presented with impaired ovarian function, high basal follicle-stimulating hormone, and high follicle-stimulating hormone levels after clomiphene stimulation on cycle day 10. In the remaining 30 women, showing an in-phase endometrium after CC stimulation, a comparison of six morphological characteristics did not reveal any significant differences between the 14 women who did become pregnant and the 16 who did not. No significant differences in endometrial thickness were observed between the groups. Significant differences were found when comparing estradiol and progesterone area under the curve during the luteal phase (P < 0.001 and P < 0.01, respectively) between those who did and those who did not become pregnant. CONCLUSIONS: Luteal endometrium morphology was not a sharp instrument to detect differences between women who did and women who did not become pregnant following IVF treatment, while ovarian function, as measured by hormonal markers, seemed to be a more reliable prognostic factor for IVF treatment outcome. (+info)Malignant transformation of human prostatic epithelium is associated with the loss of androgen receptor immunoreactivity in the surrounding stroma. (8/4669)
The cellular pathways involved in the pathogenesis of hormone resistance remain unclear. Studies evaluating the role of changes in human androgen receptor (hAR) expression in the progression of prostatic tumors have been inconclusive. Androgenic influence over prostatic growth is mediated via the regulation of interactions between stromal and epithelial cells. We hypothesized that neoplastic transformation of the prostate would be associated with alterations in hAR expression in the adjacent stroma. Using immunohistochemical techniques, we determined hAR positivity in the epithelium and adjacent stroma of sections from 17 benign and 39 malignant prostatic glands. We found that whereas the expression of the receptor decreased in both cellular compartments as the tissues dedifferentiated, the depletion was more pronounced in the stromal nuclei (P<0.0001). However, in sections from both untreated and hormone-resistant prostate cancer tissues, although heterogeneity of hAR expression in malignant epithelia was increased, there appeared to be a unique field effect around the cancerous prostate glands that resulted in a decreased expression of the receptor in the adjacent benign glands and its total loss in the surrounding stroma. The loss of hAR in the stroma adjacent to malignant prostatic epithelium may play an important role in prostate cancer progression. Furthermore, the similarity of the lack of stromal hAR expression in newly diagnosed and hormone-resistant prostate cancer (P = 0.85) may be an indication that the mechanisms responsible for the acquisition of hormone independence are established early in the malignant transformation process. (+info)The symptoms of chlamydia infections can vary depending on the location of the infection. In genital infections, symptoms may include:
* Discharge from the penis or vagina
* Painful urination
* Abnormal bleeding or spotting
* Painful sex
* Testicular pain in men
* Pelvic pain in women
In eye infections, symptoms can include:
* Redness and swelling of the eye
* Discharge from the eye
* Pain or sensitivity to light
In respiratory infections, symptoms may include:
* Cough
* Fever
* Shortness of breath or wheezing
If left untreated, chlamydia infections can lead to serious complications, such as pelvic inflammatory disease (PID) in women and epididymitis in men. Chlamydia infections can also increase the risk of infertility and other long-term health problems.
Chlamydia infections are typically diagnosed through a physical examination, medical history, and laboratory tests such as a nucleic acid amplification test (NAAT) or a culture test. Treatment for chlamydia infections typically involves antibiotics, which can effectively cure the infection. It is important to note that sexual partners of someone with a chlamydia infection should also be tested and treated, as they may also have the infection.
Prevention methods for chlamydia infections include safe sex practices such as using condoms and dental dams, as well as regular screening and testing for the infection. It is important to note that chlamydia infections can be asymptomatic, so regular testing is crucial for early detection and treatment.
In conclusion, chlamydia is a common sexually transmitted bacterial infection that can cause serious complications if left untreated. Early detection and treatment are key to preventing long-term health problems and the spread of the infection. Safe sex practices and regular screening are also important for preventing chlamydia infections.
Gastroenteritis can be classified into different types based on the cause:
Viral gastroenteritis - This is the most common type of gastroenteritis and is caused by norovirus or rotavirus.
Bacterial gastroenteritis - This type is caused by bacteria such as salmonella, E. coli, or campylobacter.
Parasitic gastroenteritis - This is caused by parasites such as giardia or cryptosporidium.
Foodborne gastroenteritis - This type is caused by consuming contaminated food or water.
Gastroenteritis can be treated with antibiotics for bacterial infections, anti-diarrheal medications, and hydration therapy to prevent dehydration. In severe cases, hospitalization may be necessary.
Prevention measures include proper hand washing, avoiding close contact with people who are sick, and avoiding contaminated food and water. Vaccines are also available for some types of gastroenteritis such as rotavirus.
Source: 'Rubella' in Duane Gubler (ed.), up-to-date online clinical reference, retrieved on March 14, 2023 from
The symptoms of rotavirus infection can range from mild to severe and may include:
* Diarrhea
* Vomiting
* Fever
* Abdominal pain
* Dehydration
* Loss of appetite
* Weight loss
In severe cases, rotavirus infection can lead to complications such as:
* Dehydration
* Malnutrition
* Electrolyte imbalance
* Acute kidney injury
* Septicemia
* Death (rare)
The diagnosis of rotavirus infection is based on a combination of clinical symptoms, laboratory tests, and medical imaging. Laboratory tests may include:
* Stool testing for the presence of rotavirus antigens or genetic material
* Blood testing for signs of dehydration or electrolyte imbalance
There is no specific treatment for rotavirus infection, but rather supportive care to manage symptoms and prevent complications. This may include:
* Fluid replacement therapy to prevent dehydration
* Anti-diarrheal medications to slow down bowel movements
* Pain management with medication
* Rest and hydration
Prevention is key in managing rotavirus infections. Vaccines are available to protect against rotavirus infection, and good hygiene practices such as frequent handwashing and avoiding close contact with people who are sick can also help prevent the spread of the virus.
Overall, while rotavirus infections can be severe and potentially life-threatening, with proper supportive care and prevention measures, most children recover fully within a few days to a week.
PSE can be a serious condition, especially in older adults or those with weakened immune systems, as it can lead to life-threatening complications such as inflammation of the bowel wall, perforation of the bowel, and sepsis. PSE is often diagnosed through a combination of clinical symptoms, laboratory tests, and imaging studies such as X-rays or CT scans. Treatment typically involves antibiotics to eradicate the infection, as well as supportive care to manage symptoms such as fluid replacement, pain management, and wound care. In severe cases, surgery may be necessary to remove damaged portions of the intestine.
Prevention measures for PSE include proper hand hygiene, isolation precautions, and environmental cleaning to reduce the transmission of C. diff spores. Probiotics, which are live microorganisms that are similar to the beneficial bacteria found in the gut, have also been shown to be effective in preventing PSE recurrence.
Giardiasis is a disease caused by the protozoan parasite Giardia duodenalis, which is found in contaminated water, food, or direct contact with infected individuals. The parasite enters the small intestine and feeds on the mucosal lining, causing inflammation, diarrhea, and abdominal cramps.
Prevalence:
Giardiasis is a common disease worldwide, affecting approximately 500 million people annually, with higher prevalence in developing countries. In the United States, it is estimated that over 1.5 million people are infected each year, with the highest incidence rates found among children and travelers to endemic areas.
Symptoms:
The symptoms of giardiasis can vary in severity but typically include:
* Diarrhea (sometimes bloody)
* Abdominal cramps
* Weight loss
* Fatigue
* Nausea and vomiting
* Fever
* Headache
In some cases, the infection can lead to more severe complications such as:
* Malabsorption (deficiency of essential nutrients)
* Inflammation of the intestine
* Rectal prolapse
Diagnosis:
The diagnosis of giardiasis is based on a combination of clinical symptoms, laboratory tests, and medical history. The most common diagnostic techniques include:
* Microscopic examination of stool samples for the presence of Giardia eggs or trophozoites
* Enzyme-linked immunosorbent assay (ELISA) to detect antigens or antibodies against Giardia in stool or blood samples
* Polymerase chain reaction (PCR) to detect the parasite's DNA in stool samples
Treatment:
The treatment of giardiasis typically involves the use of antiparasitic drugs, such as metronidazole or tinidazole. These medications are effective against the parasite and can be administered orally or intravenously, depending on the severity of the infection. The duration of treatment varies depending on the individual case, but it is generally between 5-10 days.
Prevention:
Preventing giardiasis involves avoiding exposure to contaminated water or food sources. Some measures that can be taken to prevent the infection include:
* Avoiding consumption of untreated water, especially when traveling to areas with poor sanitation
* Avoiding contact with people who have diarrhea or are infected with Giardia
* Properly storing and cooking food to kill any parasites that may be present
* Avoiding raw or undercooked meat, especially pork and wild game
* Washing hands frequently, especially before eating or preparing food
It is important to note that giardiasis can be a recurring infection, so it is important to take preventive measures consistently.
Symptoms of cervicitis may include:
* Pain or discomfort during sex
* Vaginal bleeding or spotting
* Abnormal vaginal discharge
* Itching or burning sensation in the vagina
* Pain or pressure in the lower abdomen
To diagnose cervicitis, a healthcare provider may perform a physical examination and may also use tests such as a pelvic examination, Pap smear, or vaginal swab culture to rule out other conditions.
Treatment for cervicitis typically involves antibiotics if the condition is caused by a bacterial infection. If the cause is an STI, treatment may involve antiviral or antibacterial medication. In addition, home remedies such as applying warm compresses to the area, taking over-the-counter pain relievers, and avoiding sexual intercourse until the symptoms resolve can be helpful.
Preventive measures for cervicitis include:
* Practicing safe sex by using condoms or other barrier methods
* Getting regular Pap smears to detect any abnormal cell changes in the cervix
* Avoiding douching, as it can disrupt the natural balance of bacteria in the vagina and lead to infection
* Wearing breathable cotton underwear and avoiding tight-fitting clothing that can trap moisture and bacteria close to the skin.
Immunoassay
Chemiluminescent immunoassay
Enzyme immunoassay
Magnetic immunoassay
Fluorescence polarization immunoassay
Mass spectrometric immunoassay
Turbidimetric inhibition immunoassay
Cloned enzyme donor immunoassay
Enzyme multiplied immunoassay technique
Surround optical-fiber immunoassay
International unit
Trinder glucose activity test
Hook effect
Human anti-mouse antibody
Clearblue
CSPD (molecule)
Antibody microarray
Cystatin C
Amniotic sac
Ara h1
Proteomics
Triad (environmental science)
Thyroid function tests
Campylobacter fetus
QuidelOrtho
AKR1B1
Digital microfluidics
Erythroferrone
Adeyinka Gladys Falusi
Droplet-based microfluidics
Explore more below...
Immunoassay Webinars | Beckman Coulter
Detectable by commercial radioimmunoassay or enzyme immunoassay
tests. Carbon Monoxide Poisoning -- South Dakota
Japanese Encephalitis Workup: Approach Considerations, Lumbar Puncture, Immunoassays
Browsing by Subject "Immunoassay"
State of the art of immunoassay methods for B-type natriuretic peptides: An update
UniCel DxI 600 Access Immunoassay System from Beckman Coulter : Get Quote, RFQ, Price or Buy
Immunoassays for the Evaluation of Antiphospholipid Syndrome (APS) | AACC.org
Immunoassay control method based on light scattering | (1999) | Bilyi | Publications | Spie
Kavain Interference with Amphetamine Immunoassay - PubMed
Patent Detail: Microchannel Magneeto-Immunoassay (Superfund Research Program)
28.2 Bn Cancer Biomarkers Markets, 2026 by Profiling Technology (Omics, Imaging, Immunoassay, Bioinformatics), & Application ...
Rapid Field Immunoassay for Detecting Antibody to Sin Nombre Virus in Deer Mice - Volume 13, Number 10-October 2007 - Emerging...
Critical Binding Reagents in Immunoassays: The Key to Precision, Consistency, and Success
Dried Blood Spots - Preparing and Processing for Use in Immunoassays and in Molecular Techniques | Protocol (Translated to...
Rat Cytokine/Chemokine Immunoassay Panel
Diagnostic Microbiology & Infectious Disease Products | Buy Molecular Controls MDx, Immunoassay Solutions IDx & Over 1200...
Glucagon-Like Peptide-2 - Competitive ELISA, Immobilized antibody - Immunoassays | BioVendor Research and Diagnostics Products
Diabetes Laboratory Immunoassays Market 2023 Segments Analysis by Top Key Players :Bayer AG, Jei Daniel Biotech Corp., Becton...
Nano-immunoassay with improved performance for detection of cancer biomarkers.
Bio-Plex Multiplex Immunoassay Publications Library | Bio-Rad
Immunoassay - Whitepapers - Siemens Healthineers Portugal
Immunoassay Technology - Clinical Trials Arena
Developing and implementing nano-immunoassays to advance quantitative proteomic analysis, biomarker assessment and molecular...
A biomimetic engineered bone platform for advanced testing of prosthetic implants | Scientific Reports
Immunoassay Testing | Redhills
Enzyme Immunoassay - Bio EMM
A duplex recombinant viral nucleoprotein microbead immunoassay for simultaneous detection of seroresponses to human respiratory...
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dot-immunoassay | Chemistry. Biology. Ecology
Enzyme immunoassays1
- IgM dot enzyme immunoassays for CSF and serum are simple, portable tests that compare favorably with capture ELISA for field diagnosis (sensitivity of 98.3% and specificity of 99.2% when compared with capture ELISA as the standard). (medscape.com)
Fluorescent Immunoassay2
- The Sofia 2 SARS Antigen Fluorescent Immunoassay (FIA) uses advanced immunofluorescence-based lateral flow technology in a sandwich design for qualitative detection of nucleocapsid protein from SARS-CoV-2. (who.int)
- Two unique test cartridges contain a single-use, digital fluorescent immunoassay antigen test that returns accurate results in 15 minutes or less. (nih.gov)
Beckman Coulter3
- Urine immunoassay was performed on a Beckman Coulter AU480 Analyser using CEDIA amphetamine-type substances reagent and DRI ethanol reagent. (nih.gov)
- The DxI 600 uses the same reagents as other UniCel Access systems, so your laboratory can deliver consistent patient results, no matter which Beckman Coulter immunoassay system performs the test. (news-medical.net)
- To determine if the presence of MDPV can produce a false-positive PCP immunoassay result by the Synchron system, the author requested that an MDPV 'spiking' experiment be conducted at ThermoFisher's California laboratory using the PCP reagent manufactured for Beckman Coulter by ThermoFisher on the Beckman Coulter DxC analyzer. (erowid.org)
ELISA3
- Japanese encephalitis virus-specific IgM capture-enzyme-linked immunoassay (ELISA) on serum or CSF is the standard diagnostic test for Japanese encephalitis. (medscape.com)
- Recently, we acquired and implemented the Luminex xMAP immunoassay system, which is a multiplex in-solution ELISA assay system providing complementary assays to the Simple Western platform (such as cytokine, metabolite, immune response, serum/plasma biomarker analysis, etc. (nih.gov)
- ELISA stands for enzyme-linked immunoassay. (medlineplus.gov)
Reagents2
- With up to 50 reagents on-board, the UniCel DxI 600 Access Immunoassay System enables you have the flexibility to run both routine and specialized tests with amazing simplicity. (news-medical.net)
- In this white paper we discuss the importance of critical binding reagents and provide detailed information on critical reagent preparation, labeling, and characterization in immunoassays. (worldwide.com)
Multiplex3
- Bastarache JA, Koyama T, Wickersham NE, Ware LB. Validation of a multiplex electrochemiluminescent immunoassay platform in human and mouse samples. (nih.gov)
- BACKGROUND: Despite the widespread use of multiplex immunoassays, there are very few scientific reports that test the accuracy and reliability of a platform prior to publication of experimental data. (nih.gov)
- METHODS: In this study, our goal was to test the accuracy and reproducibility of an electrochemiluminescent multiplex immunoassay platform (Meso Scale Discovery, MSD®) and compare this platform to validated, singleplex immunoassays (R&D Systems®) using actual study subject (human plasma and mouse bronchoalveolar lavage fluid (BAL) and plasma) samples. (nih.gov)
Antibody2
- The diagnosis of Japanese encephalitis is supported by a capture immunoassay methodology demonstrating IgM antibody in the CSF or the serum. (medscape.com)
- We developed a 1-hour field enzyme immunoassay (EIA) for detecting antibody to Sin Nombre virus in deer mice ( Peromyscus maniculatus ). (cdc.gov)
Falsely2
- However, when analysed by GCMS they were negative for amphetamine-type but contained kavain.The kava powders and kavain standard all cross reacted with the amphetamine immunoassay to give falsely detected results. (nih.gov)
- Immunoassay Measurement of Serum Estradiol: fulvestrant injection can interfere with estradiol measurement by immunoassay, resulting in falsely elevated estradiol levels. (nih.gov)
Lateral flow4
- One is a lateral flow immunoassay with a reader which delivers electronic results intended to be used in point-of-care settings. (nih.gov)
- A lateral flow immunoassay capable of detecting SARS-CoV-2 with visually read results in 20 minutes. (nih.gov)
- A single-use, lateral-flow test strip immunoassay that can provide results in 15 minutes or less. (nih.gov)
- The Sofia test kit, a lateral flow immunoassay, is used with Sofia and Sofia 2 Analyzers which give electronic results within 15 minutes. (nih.gov)
Detection5
- The 2006 revised Sapporo laboratory criteria for APS as previously mentioned includes the lupus anticoagulant tests as well as immunoassays for the detection of IgG and IgM antibodies to cardiolipin (CL) and β2GPI. (aacc.org)
- I will be addressing the rationale for these suggestions during the course of this presentation with a focus on the immunoassays for the detection of IgG and IgM antibodies to CL and β2GPI. (aacc.org)
- Nano-immunoassay with improved performance for detection of cancer biomarkers. (nih.gov)
- Nano-immunoassay utilizing surface-enhanced Raman scattering (SERS) effect is a promising analytical technique for early detection of cancer. (nih.gov)
- Title : A duplex recombinant viral nucleoprotein microbead immunoassay for simultaneous detection of seroresponses to human respiratory syncytial virus and metapneumovirus infections* Personal Author(s) : Zhang, Yange;Brooks, W. Abdullah;Goswami, Doli;Rahman, Mustafizur;Luby, Stephen P.;Erdman, Dean D. (cdc.gov)
Diagnosis1
- Performance of LC-MS/MS and immunoassay based 24-h urine free cortisol in the diagnosis of Cushing's syndrome. (bvsalud.org)
Urine3
- We encountered unexpected false positive urine results in three patients for amphetamine-type substances by immunoassay, measured as part of community drug prevention programmes. (nih.gov)
- Three different kava powders were purchased from local kava clubs and dissolved in ethanol, then evaporated and reconstituted in blank urine and analysed by immunoassay, GC-MS for amphetamine-type substances. (nih.gov)
- The patient urine samples tested positive by CEDIA immunoassay for amphetamines. (nih.gov)
Biomarkers1
- Based on profiling technologies, the cancer biomarkers market has been segmented into omics technologies, imaging technologies, immunoassay, bioinformatics and cytogenetics. (dutchnews.nl)
Hormone1
- Comparability of antim?llerian hormone levels among commercially available immunoassays. (ucdenver.edu)
Sensitivity1
- To address the challenges of conventional proteomic approaches, such as poor sensitivity and assay reproducibility, unreliable data quantification and lack of assay robustness, we have recently developed and implemented Simple Western immunoassays in the CCR Collaborative Protein Technology Resource core. (nih.gov)
Methods5
- The aim of this review article is to give an update on the state of the art of the immunoassay methods for the measurement of B-type natriuretic peptide (BNP) and its related peptides. (nih.gov)
- Because BNP immunoassay methods show large (up to 50%) systematic differences in values, the use of identical decision values for all immunoassay methods, as suggested by the most recent international guidelines, seems unreasonable. (nih.gov)
- Since proBNP significantly cross-reacts with all commercial immunoassay methods considered specific for BNP, manufacturers should test and clearly declare the degree of cross-reactivity of glycosylated and non-glycosylated proBNP in their BNP immunoassay methods. (nih.gov)
- Clinicians should take into account that there are large systematic differences between methods when they compare results from different laboratories that use different BNP immunoassays. (nih.gov)
- ROC analysis revealed the highest AUC for one of the immunoassays , although differences between the three methods were not significant. (bvsalud.org)
Results3
- Further investigation revealed that abusers of psychoactive Bath Salts in multiple states were presenting with reactive PCP screening immunoassay results. (erowid.org)
- A number of states' Department of Corrections Probation and Parole Divisions informed one of the authors (AMM) that they had also noted a recent unexplained surge in PCP-positive screening immunoassay test results. (erowid.org)
- As abuse of MDPV continues and additional designer compounds enter the psychoactive drug abuse arena, we recommend that all positive PCP screening immunoassay results be confirmed using additional analytical metho dologies (e.g. (erowid.org)
Procedure1
- Immunoassay testing is an investigative procedure to quantitatively assess and measure the presence and/or amount or functional activity of a target analyte. (redhillsrepro.com)
Laboratory1
- Our team of research specialists at Mr Accuracy Reports has published a stunning report on the Diabetes Laboratory Immunoassays Market which incorporates a 360-degree idea on its Supply Chain Analysis during the estimated period of 2023-2032. (tewahanui.info)
Technology1
- The technology is an automated capillary immunoassay platform providing quantitative profiling of proteins and their post-translational modifications. (nih.gov)
Diagnostic2
- In the present study we compared diagnostic accuracy of UFC measured with LC-MS/MS vs. immunoassay in patient with CS and patients where CS has been excluded. (bvsalud.org)
- Direct comparison with LC-MS/MS indicates that high diagnostic accuracy can be obtained with suitable immunoassays . (bvsalud.org)
Platform1
- We demonstrate that reproducibility of the platform is enhanced by using atomically smooth mica surface as a template for preparation of capture surface in SERS sandwich immunoassay. (nih.gov)
Mass2
- Additionally authentic kavain standard was also tested for cross reactivity by immunoassay and analysed by GC-MS to compare the mass fragmentation pattern and retention time with the kava powder and patient specimens. (nih.gov)
- Doubts have been raised regarding specificity of immunoassays , and mass spectrometric techniques have been proposed as an alternative. (bvsalud.org)
Details1
- See Immunoassays for more details. (medscape.com)
Tests1
- Detectable by commercial radioimmunoassay or enzyme immunoassay tests. (cdc.gov)