A technique using antibodies for identifying or quantifying a substance. Usually the substance being studied serves as antigen both in antibody production and in measurement of antibody by the test substance.
Immunologic techniques based on the use of: (1) enzyme-antibody conjugates; (2) enzyme-antigen conjugates; (3) antienzyme antibody followed by its homologous enzyme; or (4) enzyme-antienzyme complexes. These are used histologically for visualizing or labeling tissue specimens.
Fluoroimmunoassay where detection of the hapten-antibody reaction is based on measurement of the increased polarization of fluorescence-labeled hapten when it is combined with antibody. The assay is very useful for the measurement of small haptenic antigens such as drugs at low concentrations.
An immunoenzyme test for the presence of drugs and other substances in urine and blood. The test uses enzyme linked antibodies that react only with the particular drug for which the sample is being tested.
Commercially prepared reagent sets, with accessory devices, containing all of the major components and literature necessary to perform one or more designated diagnostic tests or procedures. They may be for laboratory or personal use.
Binary classification measures to assess test results. Sensitivity or recall rate is the proportion of true positives. Specificity is the probability of correctly determining the absence of a condition. (From Last, Dictionary of Epidemiology, 2d ed)
An immunoassay utilizing an antibody labeled with an enzyme marker such as horseradish peroxidase. While either the enzyme or the antibody is bound to an immunosorbent substrate, they both retain their biologic activity; the change in enzyme activity as a result of the enzyme-antibody-antigen reaction is proportional to the concentration of the antigen and can be measured spectrophotometrically or with the naked eye. Many variations of the method have been developed.
The use of fluorescence spectrometry to obtain quantitative results for the FLUORESCENT ANTIBODY TECHNIQUE. One advantage over the other methods (e.g., radioimmunoassay) is its extreme sensitivity, with a detection limit on the order of tenths of microgram/liter.
Classic quantitative assay for detection of antigen-antibody reactions using a radioactively labeled substance (radioligand) either directly or indirectly to measure the binding of the unlabeled substance to a specific antibody or other receptor system. Non-immunogenic substances (e.g., haptens) can be measured if coupled to larger carrier proteins (e.g., bovine gamma-globulin or human serum albumin) capable of inducing antibody formation.
Serological reactions in which an antiserum against one antigen reacts with a non-identical but closely related antigen.
Positive test results in subjects who do not possess the attribute for which the test is conducted. The labeling of healthy persons as diseased when screening in the detection of disease. (Last, A Dictionary of Epidemiology, 2d ed)
Studies determining the effectiveness or value of processes, personnel, and equipment, or the material on conducting such studies. For drugs and devices, CLINICAL TRIALS AS TOPIC; DRUG EVALUATION; and DRUG EVALUATION, PRECLINICAL are available.
Method of analyzing chemicals using automation.
Techniques used for determining the values of photometric parameters of light resulting from LUMINESCENCE.
Detection of drugs that have been abused, overused, or misused, including legal and illegal drugs. Urine screening is the usual method of detection.
The major immunoglobulin isotype class in normal human serum. There are several isotype subclasses of IgG, for example, IgG1, IgG2A, and IgG2B.
Chemical analysis based on the phenomenon whereby light, passing through a medium with dispersed particles of a different refractive index from that of the medium, is attenuated in intensity by scattering. In turbidimetry, the intensity of light transmitted through the medium, the unscattered light, is measured. In nephelometry, the intensity of the scattered light is measured, usually, but not necessarily, at right angles to the incident light beam.
Europium. An element of the rare earth family of metals. It has the atomic symbol Eu, atomic number 63, and atomic weight 152. Europium is used in the form of its salts as coatings for cathode ray tubes and in the form of its organic derivatives as shift reagents in NMR spectroscopy.
Antibodies produced by a single clone of cells.
The statistical reproducibility of measurements (often in a clinical context), including the testing of instrumentation or techniques to obtain reproducible results. The concept includes reproducibility of physiological measurements, which may be used to develop rules to assess probability or prognosis, or response to a stimulus; reproducibility of occurrence of a condition; and reproducibility of experimental results.
The property of antibodies which enables them to react with some ANTIGENIC DETERMINANTS and not with others. Specificity is dependent on chemical composition, physical forces, and molecular structure at the binding site.
Immunoglobulins produced in response to VIRAL ANTIGENS.
A microanalytical technique combining mass spectrometry and gas chromatography for the qualitative as well as quantitative determinations of compounds.
A class of immunoglobulin bearing mu chains (IMMUNOGLOBULIN MU-CHAINS). IgM can fix COMPLEMENT. The name comes from its high molecular weight and originally being called a macroglobulin.
Negative test results in subjects who possess the attribute for which the test is conducted. The labeling of diseased persons as healthy when screening in the detection of disease. (Last, A Dictionary of Epidemiology, 2d ed)
Antibodies that are chemically bound to a substrate material which renders their location fixed.
Passive agglutination tests in which antigen is adsorbed onto latex particles which then clump in the presence of antibody specific for the adsorbed antigen. (From Stedman, 26th ed)
A cardiotonic glycoside obtained mainly from Digitalis lanata; it consists of three sugars and the aglycone DIGOXIGENIN. Digoxin has positive inotropic and negative chronotropic activity. It is used to control ventricular rate in ATRIAL FIBRILLATION and in the management of congestive heart failure with atrial fibrillation. Its use in congestive heart failure and sinus rhythm is less certain. The margin between toxic and therapeutic doses is small. (From Martindale, The Extra Pharmacopoeia, 30th ed, p666)
A system for verifying and maintaining a desired level of quality in a product or process by careful planning, use of proper equipment, continued inspection, and corrective action as required. (Random House Unabridged Dictionary, 2d ed)
A basis of value established for the measure of quantity, weight, extent or quality, e.g. weight standards, standard solutions, methods, techniques, and procedures used in diagnosis and therapy.
Diagnostic procedures involving immunoglobulin reactions.
Excrement from the INTESTINES, containing unabsorbed solids, waste products, secretions, and BACTERIA of the DIGESTIVE SYSTEM.
Determination, by measurement or comparison with a standard, of the correct value of each scale reading on a meter or other measuring instrument; or determination of the settings of a control device that correspond to particular values of voltage, current, frequency or other output.
Substances used for the detection, identification, analysis, etc. of chemical, biological, or pathologic processes or conditions. Indicators are substances that change in physical appearance, e.g., color, at or approaching the endpoint of a chemical titration, e.g., on the passage between acidity and alkalinity. Reagents are substances used for the detection or determination of another substance by chemical or microscopical means, especially analysis. Types of reagents are precipitants, solvents, oxidizers, reducers, fluxes, and colorimetric reagents. (From Grant & Hackh's Chemical Dictionary, 5th ed, p301, p499)
Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
Immunoglobulin molecules having a specific amino acid sequence by virtue of which they interact only with the ANTIGEN (or a very similar shape) that induced their synthesis in cells of the lymphoid series (especially PLASMA CELLS).
Immunoglobulins produced in a response to BACTERIAL ANTIGENS.
Techniques used to carry out clinical investigative procedures in the diagnosis and therapy of disease.
Small uniformly-sized spherical particles, of micrometer dimensions, frequently labeled with radioisotopes or various reagents acting as tags or markers.
The range or frequency distribution of a measurement in a population (of organisms, organs or things) that has not been selected for the presence of disease or abnormality.
Measurable and quantifiable biological parameters (e.g., specific enzyme concentration, specific hormone concentration, specific gene phenotype distribution in a population, presence of biological substances) which serve as indices for health- and physiology-related assessments, such as disease risk, psychiatric disorders, environmental exposure and its effects, disease diagnosis, metabolic processes, substance abuse, pregnancy, cell line development, epidemiologic studies, etc.
The development and use of techniques and equipment to study or perform chemical reactions, with small quantities of materials, frequently less than a milligram or a milliliter.
Substances elaborated by viruses that have antigenic activity.
Liquid chromatographic techniques which feature high inlet pressures, high sensitivity, and high speed.
An anticonvulsant that is used to treat a wide variety of seizures. It is also an anti-arrhythmic and a muscle relaxant. The mechanism of therapeutic action is not clear, although several cellular actions have been described including effects on ion channels, active transport, and general membrane stabilization. The mechanism of its muscle relaxant effect appears to involve a reduction in the sensitivity of muscle spindles to stretch. Phenytoin has been proposed for several other therapeutic uses, but its use has been limited by its many adverse effects and interactions with other drugs.
Concentration or quantity that is derived from the smallest measure that can be detected with reasonable certainty for a given analytical procedure.
Substances elaborated by bacteria that have antigenic activity.
Antibodies elicited in a different species from which the antigen originated. These antibodies are directed against a wide variety of interspecies-specific antigens, the best known of which are Forssman, Hanganutziu-Deicher (H-D), and Paul-Bunnell (P-B). Incidence of antibodies to these antigens--i.e., the phenomenon of heterophile antibody response--is useful in the serodiagnosis, pathogenesis, and prognosis of infection and latent infectious states as well as in cancer classification.
Substances of fungal origin that have antigenic activity.
Measurement of the polarization of fluorescent light from solutions or microscopic specimens. It is used to provide information concerning molecular size, shape, and conformation, molecular anisotropy, electronic energy transfer, molecular interaction, including dye and coenzyme binding, and the antigen-antibody reaction.
The first alpha-globulins to appear in mammalian sera during FETAL DEVELOPMENT and the dominant serum proteins in early embryonic life.
Form of radioimmunoassay in which excess specific labeled antibody is added directly to the test antigen being measured.
Sensitive assay using radiolabeled ANTIGENS to detect specific ANTIBODIES in SERUM. The antigens are allowed to react with the serum and then precipitated using a special reagent such as PROTEIN A sepharose beads. The bound radiolabeled immunoprecipitate is then commonly analyzed by gel electrophoresis.
In screening and diagnostic tests, the probability that a person with a positive test is a true positive (i.e., has the disease), is referred to as the predictive value of a positive test; whereas, the predictive value of a negative test is the probability that the person with a negative test does not have the disease. Predictive value is related to the sensitivity and specificity of the test.
Type species of CHLAMYDIA causing a variety of ocular and urogenital diseases.
Serum that contains antibodies. It is obtained from an animal that has been immunized either by ANTIGEN injection or infection with microorganisms containing the antigen.
The type (and only) species of RUBIVIRUS causing acute infection in humans, primarily children and young adults. Humans are the only natural host. A live, attenuated vaccine is available for prophylaxis.
A milky, product excreted from the latex canals of a variety of plant species that contain cauotchouc. Latex is composed of 25-35% caoutchouc, 60-75% water, 2% protein, 2% resin, 1.5% sugar & 1% ash. RUBBER is made by the removal of water from latex.(From Concise Encyclopedia Biochemistry and Molecular Biology, 3rd ed). Hevein proteins are responsible for LATEX HYPERSENSITIVITY. Latexes are used as inert vehicles to carry antibodies or antigens in LATEX FIXATION TESTS.
Techniques used to demonstrate or measure an immune response, and to identify or measure antigens using antibodies.
Antibodies reactive with HIV ANTIGENS.
A 60-kDa extracellular protein of Streptomyces avidinii with four high-affinity biotin binding sites. Unlike AVIDIN, streptavidin has a near neutral isoelectric point and is free of carbohydrate side chains.
A genus of REOVIRIDAE, causing acute gastroenteritis in BIRDS and MAMMALS, including humans. Transmission is horizontal and by environmental contamination. Seven species (Rotaviruses A thru G) are recognized.
Infections with bacteria of the genus CHLAMYDIA.
The major hormone derived from the thyroid gland. Thyroxine is synthesized via the iodination of tyrosines (MONOIODOTYROSINE) and the coupling of iodotyrosines (DIIODOTYROSINE) in the THYROGLOBULIN. Thyroxine is released from thyroglobulin by proteolysis and secreted into the blood. Thyroxine is peripherally deiodinated to form TRIIODOTHYRONINE which exerts a broad spectrum of stimulatory effects on cell metabolism.
The application of medical knowledge to questions of law.
EPIDEMIOLOGIC STUDIES based on the detection through serological testing of characteristic change in the serum level of specific ANTIBODIES. Latent subclinical infections and carrier states can thus be detected in addition to clinically overt cases.
The clear, viscous fluid secreted by the SALIVARY GLANDS and mucous glands of the mouth. It contains MUCINS, water, organic salts, and ptylin.
Polysaccharides consisting of mannose units.
Sites on an antigen that interact with specific antibodies.
In vitro method for producing large amounts of specific DNA or RNA fragments of defined length and sequence from small amounts of short oligonucleotide flanking sequences (primers). The essential steps include thermal denaturation of the double-stranded target molecules, annealing of the primers to their complementary sequences, and extension of the annealed primers by enzymatic synthesis with DNA polymerase. The reaction is efficient, specific, and extremely sensitive. Uses for the reaction include disease diagnosis, detection of difficult-to-isolate pathogens, mutation analysis, genetic testing, DNA sequencing, and analyzing evolutionary relationships.
An enzyme isolated from horseradish which is able to act as an antigen. It is frequently used as a histochemical tracer for light and electron microscopy. Its antigenicity has permitted its use as a combined antigen and marker in experimental immunology.
Procedures for collecting, preserving, and transporting of specimens sufficiently stable to provide accurate and precise results suitable for clinical interpretation.
The status during which female mammals carry their developing young (EMBRYOS or FETUSES) in utero before birth, beginning from FERTILIZATION to BIRTH.
A form of fluorescent antibody technique utilizing a fluorochrome conjugated to an antibody, which is added directly to a tissue or cell suspension for the detection of a specific antigen. (Bennington, Saunders Dictionary & Encyclopedia of Laboratory Medicine and Technology, 1984)
The clear portion of BLOOD that is left after BLOOD COAGULATION to remove BLOOD CELLS and clotting proteins.
A method of measuring the effects of a biologically active substance using an intermediate in vivo or in vitro tissue or cell model under controlled conditions. It includes virulence studies in animal fetuses in utero, mouse convulsion bioassay of insulin, quantitation of tumor-initiator systems in mouse skin, calculation of potentiating effects of a hormonal factor in an isolated strip of contracting stomach muscle, etc.
Elements of limited time intervals, contributing to particular results or situations.
INFLAMMATION of any segment of the GASTROINTESTINAL TRACT from ESOPHAGUS to RECTUM. Causes of gastroenteritis are many including genetic, infection, HYPERSENSITIVITY, drug effects, and CANCER.
The species Oryctolagus cuniculus, in the family Leporidae, order LAGOMORPHA. Rabbits are born in burrows, furless, and with eyes and ears closed. In contrast with HARES, rabbits have 22 chromosome pairs.
Incorrect diagnoses after clinical examination or technical diagnostic procedures.
The beta subunit of human CHORIONIC GONADOTROPIN. Its structure is similar to the beta subunit of LUTEINIZING HORMONE, except for the additional 30 amino acids at the carboxy end with the associated carbohydrate residues. HCG-beta is used as a diagnostic marker for early detection of pregnancy, spontaneous abortion (ABORTION, SPONTANEOUS); ECTOPIC PREGNANCY; HYDATIDIFORM MOLE; CHORIOCARCINOMA; or DOWN SYNDROME.
A specific protein in egg albumin that interacts with BIOTIN to render it unavailable to mammals, thereby producing biotin deficiency.
The taking of a blood sample to determine its character as a whole, to identify levels of its component cells, chemicals, gases, or other constituents, to perform pathological examination, etc.
Any technique by which an unknown color is evaluated in terms of standard colors. The technique may be visual, photoelectric, or indirect by means of spectrophotometry. It is used in chemistry and physics. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)
A common inhabitant of the colon flora in human infants and sometimes in adults. It produces a toxin that causes pseudomembranous enterocolitis (ENTEROCOLITIS, PSEUDOMEMBRANOUS) in patients receiving antibiotic therapy.
Emission of LIGHT when ELECTRONS return to the electronic ground state from an excited state and lose the energy as PHOTONS. It is sometimes called cool light in contrast to INCANDESCENCE. LUMINESCENT MEASUREMENTS take advantage of this type of light emitted from LUMINESCENT AGENTS.
A measure of the binding strength between antibody and a simple hapten or antigen determinant. It depends on the closeness of stereochemical fit between antibody combining sites and antigen determinants, on the size of the area of contact between them, and on the distribution of charged and hydrophobic groups. It includes the concept of "avidity," which refers to the strength of the antigen-antibody bond after formation of reversible complexes.
A water-soluble, enzyme co-factor present in minute amounts in every living cell. It occurs mainly bound to proteins or polypeptides and is abundant in liver, kidney, pancreas, yeast, and milk.
Elements of the lanthanoid series including atomic number 57 (LANTHANUM) through atomic number 71 (LUTETIUM).
The complex formed by the binding of antigen and antibody molecules. The deposition of large antigen-antibody complexes leading to tissue damage causes IMMUNE COMPLEX DISEASES.
An acute infectious disease caused by the RUBELLA VIRUS. The virus enters the respiratory tract via airborne droplet and spreads to the LYMPHATIC SYSTEM.
Methods utilizing the principles of MICROFLUIDICS for sample handling, reagent mixing, and separation and detection of specific components in fluids.
The study of the structure, growth, function, genetics, and reproduction of viruses, and VIRUS DISEASES.
Infection with any of the rotaviruses. Specific infections include human infantile diarrhea, neonatal calf diarrhea, and epidemic diarrhea of infant mice.
Antibodies that react with self-antigens (AUTOANTIGENS) of the organism that produced them.
A glycoprotein hormone secreted by the adenohypophysis (PITUITARY GLAND, ANTERIOR). Thyrotropin stimulates THYROID GLAND by increasing the iodide transport, synthesis and release of thyroid hormones (THYROXINE and TRIIODOTHYRONINE). Thyrotropin consists of two noncovalently linked subunits, alpha and beta. Within a species, the alpha subunit is common in the pituitary glycoprotein hormones (TSH; LUTEINIZING HORMONE and FSH), but the beta subunit is unique and confers its biological specificity.
Any of a variety of procedures which use biomolecular probes to measure the presence or concentration of biological molecules, biological structures, microorganisms, etc., by translating a biochemical interaction at the probe surface into a quantifiable physical signal.
A gonadotropic glycoprotein hormone produced primarily by the PLACENTA. Similar to the pituitary LUTEINIZING HORMONE in structure and function, chorionic gonadotropin is involved in maintaining the CORPUS LUTEUM during pregnancy. CG consists of two noncovalently linked subunits, alpha and beta. Within a species, the alpha subunit is virtually identical to the alpha subunits of the three pituitary glycoprotein hormones (TSH, LH, and FSH), but the beta subunit is unique and confers its biological specificity (CHORIONIC GONADOTROPIN, BETA SUBUNIT, HUMAN).
An analytical method for detecting and measuring FLUORESCENCE in compounds or targets such as cells, proteins, or nucleotides, or targets previously labeled with FLUORESCENCE AGENTS.
Proteins prepared by recombinant DNA technology.
Antigens produced by various strains of HEPATITIS A VIRUS such as the human hepatitis A virus (HEPATITIS A VIRUS, HUMAN).
Laboratory and other services provided to patients at the bedside. These include diagnostic and laboratory testing using automated information entry.
Immunologic techniques involved in diagnosis.
The interaction of two or more substrates or ligands with the same binding site. The displacement of one by the other is used in quantitative and selective affinity measurements.
Tests that are dependent on the clumping of cells, microorganisms, or particles when mixed with specific antiserum. (From Stedman, 26th ed)
Alkaloids found in OPIUM from PAPAVER that induce analgesic and narcotic effects by action upon OPIOID RECEPTORS.
Represents 15-20% of the human serum immunoglobulins, mostly as the 4-chain polymer in humans or dimer in other mammals. Secretory IgA (IMMUNOGLOBULIN A, SECRETORY) is the main immunoglobulin in secretions.
Antibodies found in adult RHEUMATOID ARTHRITIS patients that are directed against GAMMA-CHAIN IMMUNOGLOBULINS.
A class of chemicals derived from barbituric acid or thiobarbituric acid. Many of these are GABA MODULATORS used as HYPNOTICS AND SEDATIVES, as ANESTHETICS, or as ANTICONVULSANTS.
Immunologic method used for detecting or quantifying immunoreactive substances. The substance is identified by first immobilizing it by blotting onto a membrane and then tagging it with labeled antibodies.
Chromatographic techniques in which the mobile phase is a liquid.
C(23)-steroids with methyl groups at C-10 and C-13 and a five-membered lactone at C-17. They are aglycone constituents of CARDIAC GLYCOSIDES and must have at least one double bond in the molecule. The class includes cardadienolides and cardatrienolides. Members include DIGITOXIN and DIGOXIN and their derivatives and the STROPHANTHINS.
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
Immunoglobulins raised by any form of viral hepatitis; some of these antibodies are used to diagnose the specific kind of hepatitis.
A methyl xanthine derivative from tea with diuretic, smooth muscle relaxant, bronchial dilation, cardiac and central nervous system stimulant activities. Theophylline inhibits the 3',5'-CYCLIC NUCLEOTIDE PHOSPHODIESTERASE that degrades CYCLIC AMP thus potentiates the actions of agents that act through ADENYLYL CYCLASES and cyclic AMP.
Narrow pieces of material impregnated or covered with a substance used to produce a chemical reaction. The strips are used in detecting, measuring, producing, etc., other substances. (From Dorland, 28th ed)
3 beta,12 beta,14-Trihydroxy-5 beta-card-20(22)-enolide. A cardenolide which is the aglycon of digoxin. Can be obtained by hydrolysis of digoxin or from Digitalis orientalis L. and Digitalis lanata Ehrh.
A series of steps taken in order to conduct research.
Agents that induce NARCOSIS. Narcotics include agents that cause somnolence or induced sleep (STUPOR); natural or synthetic derivatives of OPIUM or MORPHINE or any substance that has such effects. They are potent inducers of ANALGESIA and OPIOID-RELATED DISORDERS.
Drugs obtained and often manufactured illegally for the subjective effects they are said to produce. They are often distributed in urban areas, but are also available in suburban and rural areas, and tend to be grossly impure and may cause unexpected toxicity.
An intermediate in the metabolism of DIAZEPAM to OXAZEPAM. It may have actions similar to those of diazepam.
Serologic tests based on inactivation of complement by the antigen-antibody complex (stage 1). Binding of free complement can be visualized by addition of a second antigen-antibody system such as red cells and appropriate red cell antibody (hemolysin) requiring complement for its completion (stage 2). Failure of the red cells to lyse indicates that a specific antigen-antibody reaction has taken place in stage 1. If red cells lyse, free complement is present indicating no antigen-antibody reaction occurred in stage 1.
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
A mass spectrometry technique using two (MS/MS) or more mass analyzers. With two in tandem, the precursor ions are mass-selected by a first mass analyzer, and focused into a collision region where they are then fragmented into product ions which are then characterized by a second mass analyzer. A variety of techniques are used to separate the compounds, ionize them, and introduce them to the first mass analyzer. For example, for in GC-MS/MS, GAS CHROMATOGRAPHY-MASS SPECTROMETRY is involved in separating relatively small compounds by GAS CHROMATOGRAPHY prior to injecting them into an ionization chamber for the mass selection.
Analogs or derivatives of AMPHETAMINE. Many are sympathomimetics and central nervous system stimulators causing excitation, vasopressin, bronchodilation, and to varying degrees, anorexia, analepsis, nasal decongestion, and some smooth muscle relaxation.
Sensitive tests to measure certain antigens, antibodies, or viruses, using their ability to agglutinate certain erythrocytes. (From Stedman, 26th ed)
Domesticated bovine animals of the genus Bos, usually kept on a farm or ranch and used for the production of meat or dairy products or for heavy labor.
Polymerized forms of styrene used as a biocompatible material, especially in dentistry. They are thermoplastic and are used as insulators, for injection molding and casting, as sheets, plates, rods, rigid forms and beads.
An acute inflammation of the INTESTINAL MUCOSA that is characterized by the presence of pseudomembranes or plaques in the SMALL INTESTINE (pseudomembranous enteritis) and the LARGE INTESTINE (pseudomembranous colitis). It is commonly associated with antibiotic therapy and CLOSTRIDIUM DIFFICILE colonization.
An opioid analgesic with actions and uses similar to those of MORPHINE, apart from an absence of cough suppressant activity. It is used in the treatment of moderate to severe pain, including pain in obstetrics. It may also be used as an adjunct to anesthesia. (From Martindale, The Extra Pharmacopoeia, 30th ed, p1092)
A glycoprotein that is secreted into the luminal surface of the epithelia in the gastrointestinal tract. It is found in the feces and pancreaticobiliary secretions and is used to monitor the response to colon cancer treatment.
The processes triggered by interactions of ANTIBODIES with their ANTIGENS.
An infant during the first month after birth.
Ligand-binding assays that measure protein-protein, protein-small molecule, or protein-nucleic acid interactions using a very large set of capturing molecules, i.e., those attached separately on a solid support, to measure the presence or interaction of target molecules in the sample.
A family of galactoside hydrolases that hydrolyze compounds with an O-galactosyl linkage. EC 3.2.1.-.
Those hepatitis B antigens found on the surface of the Dane particle and on the 20 nm spherical and tubular particles. Several subspecificities of the surface antigen are known. These were formerly called the Australia antigen.
An examination of chemicals in the blood.
An inactive metabolite of PROGESTERONE by reduction at C5, C3, and C20 position. Pregnanediol has two hydroxyl groups, at 3-alpha and 20-alpha. It is detectable in URINE after OVULATION and is found in great quantities in the pregnancy urine.
Controlled operation of an apparatus, process, or system by mechanical or electronic devices that take the place of human organs of observation, effort, and decision. (From Webster's Collegiate Dictionary, 1993)
Immunoglobulins produced in a response to PROTOZOAN ANTIGENS.
An enzyme that catalyzes the conversion of an orthophosphoric monoester and water to an alcohol and orthophosphate. EC
The study of fluid channels and chambers of tiny dimensions of tens to hundreds of micrometers and volumes of nanoliters or picoliters. This is of interest in biological MICROCIRCULATION and used in MICROCHEMISTRY and INVESTIGATIVE TECHNIQUES.
The causative agent of venereal and non-venereal syphilis as well as yaws.
A complex of closely related aminoglycosides obtained from MICROMONOSPORA purpurea and related species. They are broad-spectrum antibiotics, but may cause ear and kidney damage. They act to inhibit PROTEIN BIOSYNTHESIS.
Studies which start with the identification of persons with a disease of interest and a control (comparison, referent) group without the disease. The relationship of an attribute to the disease is examined by comparing diseased and non-diseased persons with regard to the frequency or levels of the attribute in each group.
Blood proteins that bind to THYROID HORMONES such as THYROXINE and transport them throughout the circulatory system.
Silicone polymers which consist of silicon atoms substituted with methyl groups and linked by oxygen atoms. They comprise a series of biocompatible materials used as liquids, gels or solids; as film for artificial membranes, gels for implants, and liquids for drug vehicles; and as antifoaming agents.
A major metabolite of PROCAINAMIDE. Its anti-arrhythmic action may cause cardiac toxicity in kidney failure.
Examination of urine by chemical, physical, or microscopic means. Routine urinalysis usually includes performing chemical screening tests, determining specific gravity, observing any unusual color or odor, screening for bacteriuria, and examining the sediment microscopically.
Partial proteins formed by partial hydrolysis of complete proteins or generated through PROTEIN ENGINEERING techniques.
A genus of small, circular RNA viruses in the family ASTROVIRIDAE. They cause GASTROENTERITIS and are found in the stools of several vertebrates including humans. Transmission is by the fecal-oral route and there are at least eight human serotypes. The type species is Human astrovirus.
The top portion of the pharynx situated posterior to the nose and superior to the SOFT PALATE. The nasopharynx is the posterior extension of the nasal cavities and has a respiratory function.
The process of observing, recording, or detecting the effects of a chemical substance administered to an individual therapeutically or diagnostically.
An infection of the SMALL INTESTINE caused by the flagellated protozoan GIARDIA LAMBLIA. It is spread via contaminated food and water and by direct person-to-person contact.
The specialty of ANALYTIC CHEMISTRY applied to assays of physiologically important substances found in blood, urine, tissues, and other biological fluids for the purpose of aiding the physician in making a diagnosis or following therapy.
An analytical method used in determining the identity of a chemical based on its mass using mass analyzers/mass spectrometers.
Technique involving the diffusion of antigen or antibody through a semisolid medium, usually agar or agarose gel, with the result being a precipitin reaction.
Antigens associated with specific proteins of the human adult T-cell immunodeficiency virus (HIV); also called HTLV-III-associated and lymphadenopathy-associated virus (LAV) antigens.
A group of enzymes that catalyzes the hydrolysis of terminal, non-reducing beta-D-galactose residues in beta-galactosides. Deficiency of beta-Galactosidase A1 may cause GANGLIOSIDOSIS, GM1.
Inflammation of the UTERINE CERVIX.
Substances that are toxic to the intestinal tract causing vomiting, diarrhea, etc.; most common enterotoxins are produced by bacteria.

Immunological comparison of the proteins of chicken and rat liver ribosomes. (1/4669)

A comparison of the proteins of chicken and rat liver ribosomes using immunochemical techniques was undertaken. The procedures included quantitative precipitation, passive hemagglutination, and immunodiffusion on Ouchterlony plates. The results indicate that antisera specific for chicken or rat liver ribosomes recognize only about 20% of common determinants. While there are important reservations, the results suggest extensive differences in the proteins of rat and chicken liver ribosomes. Despite those differences, rat and chicken liver ribosomal proteins maintain some homologous sequences present in bacterial ribosomal proteins. An enriched antibody preparation against chicken 80 S ribosomes inhibited the poly(U)-directed synthesis of polyphenylalanine and the elongation factor G (EF-G)-catalyzed binding of [3H]GDP to Escherichia coli ribosomes. Thus, chicken liver ribosomes, like ribosomes from rat liver and yeast, must have proteins homologous with those of E. coli ribosomes.  (+info)

Performance and specificity of monoclonal immunoassays for cyclosporine monitoring: how specific is specific? (2/4669)

BACKGROUND: Immunoassays designed for the selective measurement of cyclosporin A (CsA) inadvertently show cross-reactivity for CsA metabolites. The extent and clinical significance of the resulting overestimation is controversial. A comprehensive assessment of old and new methods in clinical specimens is needed. METHODS: In a comprehensive evaluation, CsA was analyzed in 145 samples with the new CEDIA assay and compared with the Emit assay with the old and new pretreatments, the TDx monoclonal and polyclonal assays, the AxSYM, and HPLC. All samples were from patients with liver and/or kidney transplants. RESULTS: The CEDIA offered the easiest handling, followed by the AxSYM, which showed the longest calibration stability. The TDx monoclonal assay provided the lowest detection limit and the lowest CVs. The mean differences compared with HPLC were as follows: Emit, 9-12%; CEDIA, 18%; AxSYM, 29%; and TDx monoclonal, 57%. The CycloTrac RIA paralleled the Emit results. In contrast to the mean differences, substantial (>200%) and variable overestimations of the CsA concentration were observed in individual patient samples. Metabolic ratios, estimates of the overall concentrations of several cross-reacting metabolites (nonspecific TDx polyclonal/specific reference method), correlated with the apparent biases of the various monoclonal assays. Metabolic ratios varied up to 10-fold, which translated into biases for individual samples between -7% and +174%. The higher the cross-reactivity of an assay was, the higher was the range of biases observed. The interindividual differences markedly exceeded other factors of influence (organ transplanted, hepatic function). CONCLUSION: Because assay bias cannot be predicted in individual samples, substantially erratic CsA dosing can result. The specificity of CsA assays for parent CsA remains a major concern.  (+info)

Automated homogeneous immunoassay for gentamicin on the dimension clinical chemistry system. (3/4669)

BACKGROUND: Monitoring of the concentration of gentamicin in serum and plasma during therapy is widely recommended and practiced in hospitals. Our aim was to develop a homogeneous immunoassay based on particle-enhanced turbidimetric inhibition immunoassay technology to quantify gentamicin on the Dimension clinical chemistry system. METHODS: Assay performance was assessed on each of the Dimension models in a 15-instrument interlaboratory comparison study. A split-sample comparison (n = 1171) was also performed between the gentamicin methods on the Dimension system and the Abbott TDx analyzer, using multiple reagent and calibrator lots on multiple instruments. RESULTS: The Dimension method was linear to 25.1 micromol/L (12.0 microg/mL) with a detection limit of 0.63 micromol/L (0.3 microg/mL). Calibration was stable for 30 days. The within-run imprecision (CV) was <1.3%, and total imprecision ranged from 1.8% to 3.2% between 4.2 micromol/L (2.0 microg/mL) and 16.7 micromol/L (8.0 microg/mL) gentamicin. Linear regression analysis of the results on the Dimension method (DM) vs the Abbott TDx yielded the following equation: DM = 0.98TDx - 0.42; r = 0.987. Minimal interference was observed from structurally related compounds such as sagamicin, netilmicin, and sisomicin. CONCLUSION: The monoclonal antibody used in this method has similar reactivities toward the individual gentamicin subspecies C1, C1a, and C2, thus providing analytical recovery not significantly dependent on relative subspecies concentrations.  (+info)

Serum IGF-binding protein-6 and prostate specific antigen in breast cancer. (4/4669)

OBJECTIVE: Recent studies have demonstrated the presence of the IGF-binding proteins (IGFBPs) and prostate specific antigen (PSA), an IGFBP protease. in human breast tissue. We sought to investigate the differences in serum IGFs, IGFBP-1, -3 and -6, and PSA between patients with surgically proven breast cancer and patients with benign breast disease. DESIGN AND METHODS: Concentrations of IGFs, IGFBP-1, -3 and -6, and PSA were determined in the sera from 57 patients with breast cancer (CA), and 46 women with benign breast disease (BBD) using immunoassays for IGFs and IGFBPs and an ultrasensitive ELISA for PSA. RESULTS: The mean (+/- S.E.M.) serum IGFBP-6 level in the CA group, 127 (16) ng/ml, was statistically significantly lower than in the BBD group, 157 (10) ng/ml (P = 0.016). Patients with CA had an elevated geometric mean serum PSA level of 0.018 (range: 0.0015-0.107) ng/ml, compared with 0.007 (range: 0.0015-0.019) ng/ml in women with BBD (P = 0.025). Mean serum IGFBP-1 concentrations were significantly lower in the CA group, 16 (2) ng/ml, versus 37 (4) ng/ml in the BBD group (P = 0.001). Mean serum IGFBP-3 concentrations were also lower in the CA group versus the BBD group, at 1981 (65) ng/ml, versus 2603 (140) ng/ml (P = 0.002) respectively. In the CA group, statistically significant correlations between PSA and IGFBP-6 (r = 0.413; P = 0.001), and between PSA and IGFBP-1 (r = -0.329; P = 0.021) were seen. Differences in IGF-I and -II between the two groups were not statistically significant. CONCLUSION: Lower serum concentrations of IGFBP-6, -3 and -1, but higher PSA concentrations were seen in the breast cancer group, and collectively these would suggest that there is an increase in bioavailable IGF-I in breast cancer.  (+info)

Differential immunodiagnosis between cystic hydatid disease and other cross-reactive pathologies. (5/4669)

We assessed an Echinococcus granulosus hydatid fluid antigen-ELISA (EgHF-ELISA) as a serologic prescreening test for E. granulosus infections, supplemented by more specific confirmatory tests, including arc-5 immunoprecipitation and antigen B subunit 8-kD immunoblotting. The diagnostic sensitivity of the EgHF-ELISA was 91%. With regard to the test specificity of the EgHF-ELISA (overall = 82%), we observed relatively frequent cross-reactions in tumor patients (6%) and in patients with other parasitic diseases. Cestode-related cross-reactivity can be resolved by the complementary use of E. multilocularis-specific antigens or Taenia solium cysticercosis-specific immunoblotting. Immunoblotting based upon the detection of antibody reactivity to the 8-kD antigen of EgHF, or if appropriately detectable, to the 29-kD and 34-kD bands exhibited a 91% diagnostic sensitivity and an overall specificity of 97% or 94%, respectively. Thus, immunoblotting provided a 99% discrimination between seropositive pre-operative cystic hydatid disease cases and cross-reactive non-cestode parasitic infections or malignancies.  (+info)

An immunoluminometric assay for N-terminal pro-brain natriuretic peptide: development of a test for left ventricular dysfunction. (6/4669)

Measurement of plasma levels of brain natriuretic peptide (BNP) has been used to assess left ventricular dysfunction and prognosis. Levels of the N-terminus of the precursor of BNP (NT-proBNP) have been reported to be elevated to a greater extent than BNP in left ventricular dysfunction. We have devised a non-radioactive sensitive and specific assay for NT-proBNP based on a competitive ligand binding principle. The chemiluminescent label 4-(2-succinimidyloxycarbonylethyl)phenyl-10-methylacridinium 9-carboxylate fluorosulphonate was used to label peptides representing domains in the middle and C-terminal sections of NT-proBNP. Assay of the C-terminal section of NT-proBNP (amino acids 65-76) in patients with proven left ventricular dysfunction [left ventricular wall motion index median 0.9 (range 0.3-1.4)] revealed elevated values [median 639 (386-911) fmol/ml] compared with normal controls [left ventricular wall motion index of 2 in all, NT-proBNP median 159 (120-245) fmol/ml, P<0.001]. Measurement of the middle section of NT-proBNP (amino acids 37-49) was not a discriminating test. It is thus possible to derivatize small peptides with a methyl acridinium label and preserve immunodetection with specific antibodies. Such methodology may allow non-radioactive immunoluminometric assays to be devised.  (+info)

Endometrial evaluation is not predictive for in vitro fertilization treatment. (7/4669)

PURPOSE: The main purpose of this study was to evaluate ovarian function by clomiphene citrate (CC) challenge test in a group of tubal infertile women and to study endometrial morphological maturation in the early luteal phase of CC-stimulated cycles as compared to in vitro fertilization (IVF) treatment outcome. METHODS AND RESULTS: Four women presented with strongly retarded, proliferative endometrium in the luteal phase. Of these, three presented with impaired ovarian function, high basal follicle-stimulating hormone, and high follicle-stimulating hormone levels after clomiphene stimulation on cycle day 10. In the remaining 30 women, showing an in-phase endometrium after CC stimulation, a comparison of six morphological characteristics did not reveal any significant differences between the 14 women who did become pregnant and the 16 who did not. No significant differences in endometrial thickness were observed between the groups. Significant differences were found when comparing estradiol and progesterone area under the curve during the luteal phase (P < 0.001 and P < 0.01, respectively) between those who did and those who did not become pregnant. CONCLUSIONS: Luteal endometrium morphology was not a sharp instrument to detect differences between women who did and women who did not become pregnant following IVF treatment, while ovarian function, as measured by hormonal markers, seemed to be a more reliable prognostic factor for IVF treatment outcome.  (+info)

Malignant transformation of human prostatic epithelium is associated with the loss of androgen receptor immunoreactivity in the surrounding stroma. (8/4669)

The cellular pathways involved in the pathogenesis of hormone resistance remain unclear. Studies evaluating the role of changes in human androgen receptor (hAR) expression in the progression of prostatic tumors have been inconclusive. Androgenic influence over prostatic growth is mediated via the regulation of interactions between stromal and epithelial cells. We hypothesized that neoplastic transformation of the prostate would be associated with alterations in hAR expression in the adjacent stroma. Using immunohistochemical techniques, we determined hAR positivity in the epithelium and adjacent stroma of sections from 17 benign and 39 malignant prostatic glands. We found that whereas the expression of the receptor decreased in both cellular compartments as the tissues dedifferentiated, the depletion was more pronounced in the stromal nuclei (P<0.0001). However, in sections from both untreated and hormone-resistant prostate cancer tissues, although heterogeneity of hAR expression in malignant epithelia was increased, there appeared to be a unique field effect around the cancerous prostate glands that resulted in a decreased expression of the receptor in the adjacent benign glands and its total loss in the surrounding stroma. The loss of hAR in the stroma adjacent to malignant prostatic epithelium may play an important role in prostate cancer progression. Furthermore, the similarity of the lack of stromal hAR expression in newly diagnosed and hormone-resistant prostate cancer (P = 0.85) may be an indication that the mechanisms responsible for the acquisition of hormone independence are established early in the malignant transformation process.  (+info)

i-colloid 40 nm gold nanoparticles produced by IMRAs pulsed-laser ablation process are stabilized without surfactants or capping agents such as citrate, and conjugates for lateral flow immunoassays can be generated by high-efficiency passive adsorption of antibodies to particle surfaces. The tightly controlled laser-ablation process generates gold colloids that produce consistent lateral flow assay results. Pulsed-laser ablation also enables production of gold-alloy nanoparticles, such as gold-platinum, that can be passively conjugated like gold but have optical signatures distinct from gold due to their shifted surface plasmon resonance. Multicolor, multiplexed lateral flow immunoassays can be performed with combinations of gold and gold-alloy nanoparticles.. ...
472827490 - EP 3117213 A1 20170118 - SYSTEM AND METHOD FOR LATERAL FLOW IMMUNOASSAY TESTING - [origin: US2015260708A1] A lateral flow immunoassay system is provided that includes a housing having a base portion forming a first chamber therein and a body portion formed with three openings in fluid communication with the first chamber, a vial containing a buffer agent, and a sample collector for introducing a sample fluid into the first chamber via the second opening. The vial is mounted to the housing such that a dispensing side extends into the first opening for dispensing the buffer agent therefrom into the first chamber. The housing allows the buffer agent and sample fluid to be mixed within a reaction well formed within the first chamber to form a test sample mixture. The body portion is configured to receive a receiving end of an elongated holder in the third opening and allow a test strip secured in the holder to be brought into communication with the text sample mixture.[origin: US2015260708A1] A
Quantitative lateral flow immunoassay for total prostate specific antigen in serum / I. P. Andreeva, V. G. Grigorenko, A. M. Egorov, A. P. Osipov // Analytical Letters. - 2016. - Vol. 49, no. 4. - P. 579-588. A simple method for the rapid determination of prostate-specific antigen (PSA) in serum is reported using a lateral flow immunoassay with gold nanoparticles as the label. The method uses the intensity of colored test lines to determine PSA from 0.3 to 30 ng/ml. The limit of detection was 0.3 ng/ml and the coefficient of variation was less than 10%. The analysis time was approximately twenty minutes. The novel method showed good correlation with enzyme linked immunosorbent assay (ELISA) measurements of prostate-specific antigen concentration in human serum with a linear regression coefficient of 0.985. The developed system was stable for at least twelve months when stored from +4 to 30oC and has potential application for clinical practice. [ DOI ...
TY - GEN. T1 - Detection and Categorisation of Multilevel High-Sensitivity Cardiovascular Biomarkers from Lateral Flow Immunoassay Images via Recurrent Neural Networks. AU - Jing, Min. AU - Mc Laughlin, Donal. AU - Steele, David. AU - Mc Namee, Sara. AU - Mac Namee, Brian. AU - Cullen, Patrick. AU - Finlay, Dewar. AU - McLaughlin, James. PY - 2020/2/29. Y1 - 2020/2/29. N2 - Lateral Flow Immunoassays (LFA) have the potential to provide low cost, rapid and highly efficacious Point-of-Care (PoC) diagnostic testing in resource limited settings. Traditional LFA testing is semi-quantitative based on the calibration curve, which faces challenges in the detection of multilevel high-sensitivity biomarkers due its low sensitivity. This paper proposes a novel framework in which the LFA images are acquired from a designed CMOS reader system under controlled lighting. Unlike most existing approaches based on image intensity, the proposed system does not require detection of region of interest (ROI), instead ...
TY - JOUR. T1 - A multi-target lateral flow immunoassay enabling the specific and sensitive detection of total antibodies to SARS COV-2. AU - Cavalera, Simone. AU - Colitti, Barbara. AU - Rosati, Sergio. AU - Ferrara, Gianmarco. AU - Bertolotti, Luigi. AU - Nogarol, Chiara. AU - Guiotto, Cristina. AU - Cagnazzo, Celeste. AU - Denina, Marco. AU - Fagioli, Franca. AU - Di Nardo, Fabio. AU - Chiarello, Matteo. AU - Baggiani, Claudio. AU - Anfossi, Laura. PY - 2021/2/1. Y1 - 2021/2/1. N2 - © 2020 Elsevier B.V. A rapid test for detecting total immunoglobulins directed towards the nucleocapsid protein (N) of severe acute syndrome coronavirus 2 (SARS CoV-2) was developed, based on a multi-target lateral flow immunoassay comprising two test lines. Both test lines bound to several classes of immunoglobulins (G, M, and A). Specific anti-SARS immunoglobulins were revealed by a colorimetric probe formed by N and gold nanoparticles. Targeting the total antibodies response to infection enabled achieving 100% ...
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TY - JOUR. T1 - Concordance assessment between a multiplexed competitive Luminex immunoassay, a multiplexed IgG Luminex immunoassay, and a pseudovirion-based neutralization assay for detection of human papillomaviruse types 16 and 18. AU - Brown, Darron. AU - Müller, Martin. AU - Sehr, Peter. AU - Pawlita, Michael. AU - Seitz, Hanna. AU - Rubio, Ivonne. AU - Antonello, Joseph. AU - Radley, David. AU - Roberts, Christine. AU - Saah, Alfred. PY - 2014/10/7. Y1 - 2014/10/7. N2 - There are two approved vaccines against anogenital human papillomaviruses (HPV) and a nine-valent vaccine is currently under development. Although there are several assays available to measure antibodies elicited by HPV vaccines, there is currently no global standard for HPV antibody assays. In the current study, antibody responses to HPV16 and HPV18 among young men and women vaccinated with a quadrivalent HPV6/11/16/18 (qHPV) vaccine were assessed using three assays: a competitive Luminex immunoassay (cLIA-4) which ...
Rapid mass spectrometric immunoassay methods for detecting and/or quantifying antibody and antigen analytes utilizing affinity capture to isolate the analytes and internal reference species (for quantification) followed by mass spectrometric analysis of the isolated analyte/internal reference species. Quantification is obtained by normalizing and calibrating obtained mass spectrum against the mass spectrum obtained for an antibody/antigen of known concentration.
Procalcitonin (PCT) is a current, frequently-used marker for severe bacterial infection. The aim of this study was to develop a cost-effective detection kit for rapid quantitative and on-site detection of PCT. To develop the new PCT quantitative detecting kit, a double-antibody sandwich immunofluorescent assay was employed based on time-resolved immunofluorescent assay (TRFIA) combined with lateral flow immunoassay (LFIA). The performance of the new developed kit was evaluated in the aspects of linearity, precision, accuracy, and specificity. Two-hundred thirty-four serum samples were enrolled to carry out the comparison test. The new PCT quantitative detecting kit exhibited a higher sensitivity (0.08 ng/mL). The inter-assay coefficient of variation (CV) and the intra-assay CV were 5.4%-7.7% and 5.7%-13.4%, respectively. The recovery rates ranged from 93% to 105%. Furthermore, a high correlation (n = 234, r = 0.977, p | 0.0001) and consistency (Kappa = 0.875) were obtained when compared with the PCT kit
Rapid, simple, and cost-effective diagnostics are needed to improve healthcare at the point of care (POC). However, the most widely used POC diagnostic, the lateral flow immunoassay (LFA), is ∼1000-times less sensitive and has a smaller analytical range than laboratory tests, requiring a confirmatory test to establish truly negative results. Here, a rational and systematic strategy is used to design the LFA contrast label (i.e., gold nanoparticles) to improve the analytical sensitivity, analytical detection range, and antigen quantification of LFAs. Specifically, we discovered that the size (30, 60, or 100 nm) of the gold nanoparticles is a main contributor to the LFA analytical performance through both the degree of receptor interaction and the ultimate visual or thermal contrast signals. Using the optimal LFA design, we demonstrated the ability to improve the analytical sensitivity by 256-fold and expand the analytical detection range from 3 log10 to 6 log10 for diagnosing patients with ...
Template matching method is presented to identify the peaks from the scanned signals of lateral flow immunoassay strips. The template is composed of two pulses separated by the distance of the control and the target ligand line in the assay, and is convolved with the scanned signal to deliver the maximum at the center of the two peaks. The peak regions were identified with the predefined distances from the center. Glycosylated haemoglobin immunoassay strips and fluorescent strip readers from Boditechmed Inc. were tested to estimate the lot and reader variations of the concentration measurands. The results showed the robustness of the propose method ...
TY - JOUR. T1 - Microfluidic-based multiplex immunoassay system integrated with an array of QD-encoded microbeads. AU - Han, Sang Won. AU - Jang, Eunji. AU - Koh, Won Gun. PY - 2015/3/31. Y1 - 2015/3/31. N2 - Here, we developed a multiplex immunoassay platform within microfluidic devices that combines suspension and the planar microarray format. For the suspension microarray format, QD-embedded polymeric microbeads with an average diameter of 24 μm were prepared using the Shirasu Porous Glass (SPG) membrane emulsification technique. To furnish the microbeads with resolvable spectral codes, QDs with two different colors (450 nm for blue and 520 nm for green) were used and different spectral codes were obtained by changing the ratio of emission intensity of the two different QDs within the microbeads. The surfaces of the QD-encoded microbeads were then functionalized with probe antibodies for immunoassays. The planar microarray format was achieved by an array of microholes fabricated in PDMS. ...
The diagnosis of periprosthetic joint infection (PJI) remains a challenge. However, recent studies showed that synovial fluid biomarkers have demonstrated greater diagnostic accuracy than the currently used PJI diagnostic tests. In many diagnostic tests, combining several biomarkers into panels is critical for improving diagnostic efficiency, enhancing the diagnostic precision for specific diseases, and reducing cost. In this study, we prove that combining alpha-defensin and C-reactive protein (CRP) as biomarkers possesses the potential to provide accurate PJI diagnosis. To further verify the result, we developed a multi-target lateral flow immunoassay strip (msLFIA) with staking pad design to obtain on-site rapid response for clinical diagnosis of PJI. A total of 10 synovial fluid samples were tested using the msLFIA, and the results showed that the combined measurements of synovial fluid alpha-defensin and CRP levels were consistent with those obtained from a commercial enzyme-linked immunosorbent
This study was conducted to develop a simple, rapid, and accurate lateral flow immunoassay (LFIA) detection method for point-of-care diagnosis. The one-dot LFIA for aflatoxin B1 (AFB1) was based on the modified competitive binding format using competition between AFB1 and colloidal gold-AFB1-BSA conjugate for antibody binding sites in the test zone. A Smartphone-based reading system consisting of a Samsung Galaxy S2 Smartphone, a LFIA reader, and a Smartphone application for the image acquisition and data analysis. The detection limit of one-dot LFIA for AFB1 is 5 μg/kg. This method provided semi-quantitative analysis of AFB1 samples in the range of 5 to 1,000 μg/kg. Using combination of the one-dot LFIA and the Smartphone-based reading system, it is possible to conduct a more fast and accurate point-of-care diagnosis.
The presence of harmful algal blooms (HABs) can result in the accumulation of phycotoxins in shellfish such as mussels, scallops and oysters. Depending on the toxins present, consumption of contaminated shellfish can lead to illness in humans including amnesic, diarrheic or paralytic shellfish poisoning (ASP, DSP or PSP respectively). There is currently a lack of suitable rapid screening tools to complement accepted reference methods for the detection of marine biotoxins. The aims of the thesis were to develop a range of simple and accurate lateral flow immunoassays (LFIA) for the rapid screening of phycotoxins from shellfish extracts, which could be performed either in a laboratory or in the field. As a result, three novel single-step LFIAs were developed and validated for the major regulated toxins responsible for ASP, DSP and PSP, respectively. One of the challenges was to ensure that the thresholds for test~ were based at concentrations relevant to regulations, whilst minimizing the risks of ...
This paper is concerned with the problem of learning structure of the lateral flow immunoassay (LFIA) devices via short but available time series of the experiment measurement. The model for the LFIA is considered as a nonlinear state-space model that includes equations describing both the biochemical reaction process of LFIA system and the observation output. Especially, the time-delays occurring among the biochemical reactions are considered in the established model. Furthermore, we utilize the unscented Kalman filter (UKF) algorithm to simultaneously identify not only the states but also the parameters of the improved state-space model by using short but high-dimensional experiment data in terms of images. It is shown via experiment results that the UKF approach is particularly suitable for modelling the LFIA devices. The identified model with time-delay is of great significance for the quantitative analysis of LFIA in both the accurate prediction of the dynamic process of the concentration
China immunoassay analyzer catalog of My-B020 Auto Poct Immunoassay Analyzer Chemiluminescence Immunoassay Analyzer System, My-B020A Full Automated Chemiluminescence Immunoassay Analyzer Price provided by China manufacturer - Guangzhou Maya Medical Equipment Co., Ltd., page1.
Designed with one of the most advanced software packages on the market, the ADVIA Centaur XPT Immunoassay System is among the highest-throughput systems available. The ADVIA Centaur XPT System delivers the results that clinicians depend upon for accurate diagnoses and better patient care-and does so predictably and consistently. Siemens unites innovative workflow solutions with clinical excellence in the ADVIA Centaur XPT System, leading to greater laboratory productivity to stay ahead of increasing workload demands.. ...
TY - JOUR. T1 - Paper-based Vertical Flow Immunoassay (VFI) for detection of bio-threat pathogens. AU - Chen, Peng. AU - Gates-Hollingsworth, Marcellene. AU - Pandit, Sujata. AU - Park, Anson. AU - Montgomery, Douglas. AU - AuCoin, David. AU - Gu, Jian. AU - Zenhausern, Frederic. PY - 2019/1/1. Y1 - 2019/1/1. N2 - Currently, the standard method for identifying biological agents of potential threats to national security and public health, such as pathogens, virus, and toxins, mainly rely on microbiological cultivation. This method is time-consuming and it requires sophisticated equipment and well-trained personnel, which are often unavailable in remote areas or at point-of-need. Therefore, an alternative rapid, simple, and sensitive method for detecting bio-threat agents is in crucial need. We report a paper-based Vertical Flow Immunoassay (VFI) device that can overcome these limitations. The VFI device utilizes a nanoporous nitrocellulose membrane encapsulated in a stainless steel filter holder. ...
Read independent reviews on cAMP-Screen ® and cAMP-Screen Direct ® Chemiluminescent Immunoassay System for Determination of cAMP Concentration from Thermo Fisher Scientific on SelectScience
While lateral flow test formats can be utilized with whole blood and low sample volumes, their diagnostic characteristics are inferior to immunoassays based on chemiluminescence immunoassay (CLIA) or enzyme-linked immunosorbent assay (ELISA) technology. CLIAs and ELISAs can be automated to a high degree but commonly require larger serum or plasma volumes for sample processing. We addressed the suitability of EDTA-anticoagulated whole blood as an alternative sample material for antibody testing against SARS-CoV-2 by electro-CLIA (ECLIA; Roche, Rotkreuz, Switzerland) and ELISA (IgG and IgA; Euroimmun, Germany). Simultaneously drawn venous serum and EDTA-anticoagulated whole blood samples from 223 individuals were included. Correction of the whole blood results for hematocrit led to a good agreement with the serum results for weakly to moderately positive antibody signals. In receiver-operating characteristic curve analysis, all three assays displayed comparable diagnostic accuracy (area under the ...
The global automated immunoassay analyzer market is projected to exhibit a healthy CAGR during the forecast period. Rapid adoption of automated technology in diagnostic laboratories to spur demand for automated immunoassay analyzer. An automated immunoassay analyzer is a method or technique to spot the existence of a particular substance in the human body. It could be an antibody, antigen, hormone, pathogen, or an enzyme. Immunoassay technique is based on the principle of antibody and antigen specificity.. Growing aged population has led to high diagnostic test volumes, growing inclination toward lab automation, increasing occurrences of infectious disease. In addition, technological advancements for the development of applicable lab equipment, and growing occurrences of infectious disease epidemics such as hepatitis infection, Zika disease, HIV, and others will propel automated immunoassay analyzers in the years to come. However, lack of skilled professionals, strict regulatory framework and ...
An accurate, rapid and cost-effective detection on the environmental hazardous chemicals is the cornerstone of efficient food safe management. Hereby we discuss the relevance of an emerging technology, multiplexed competitive immunoassays read by flow cytometry, for the detection of Clenbuterol, Chloramphenicol and sulfadimidine. In these assays, multiple fluorescent microspheres, conjugated to different test antigens, constitute the solid phase for detecting antigens in biological samples. These assays are more sensitive than traditional immunoassays , have a high throughput capacity provide a wide analytical dynamic range and are powerful tools for exposure analysis and assessment offering low-cost screening with minimal sample pretreatment requirements combined and served a better alternative for the instrumental detection on the derivatives of those metabolites that often require expensive instrumentation,. Additionally, they have multiplexing ability-i. e, they are capable of measuring multiple
Farm collaborated with BioKit to help launch its first proprietary clinical analysis device, a chemiluminescent immunoassay analyzer designed to establish and enhance BioKits brand identity.
Scientists at the forefront of proteomics research can achieve exceptional accuracy and speed using an expanded family of affinity-based consumables f
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Whether you need ultrasensitive detection from a Singulex assay, maximum throughput from a Gyrolab workstation, robust ELISAs or rich profiling data from a Luminex panel, our immunoassay platforms help you get data you can trust out of each sample, with minimal repetition, so you can make the best next-step decisions.
Whether you need ultrasensitive detection from a Singulex assay, maximum throughput from a Gyrolab workstation, robust ELISAs or rich profiling data from a Luminex panel, our immunoassay platforms help you get data you can trust out of each sample, with minimal repetition, so you can make the best next-step decisions.
The statistical surveying report on the global Automatic Chemiluminescence Immunoassay Analyzer Market has been presented by using professional or expert knowledge through standard and modified...
Find FIA 8000 Quantitative Immunoassay Analyzer from China Manufacturer, Manufactory, Factory and Supplier - Nanjing Getein Biotechnology Co., Ltd. on ECVV.com
We are experts in the development of super-fast and/or super-sensitive ELISAs and multiplexed assays. Competitive immunoassays is an eagle-i resource of type Material analysis service at Charles Drew University of Medicine and Science.
The global microfluidic immunoassay market is expected to grow at a CAGR of 12.7% from 2019 to reach $2.01 billion by 2025, Microfluidic Immunoassay Market by Product Type (Lab-on-chip, POC Analyzers, Reagents & Assay), Technology, Application (Cardiac, Troponin, BNP) - Global Forecast to 2025
Mouse CD23 Multiplex Immunoassay Kit Mplex Immunoassay Kits datasheet (ab221510). Abcam offers quality products including antibodies, assays and other reagents.
Evaluation of a new competitive immunoassay (BioElisa Syphilis) for screening for Treponema pallidum antibodies at various stages of syphilis.: The BioElisa Syp
Presence of anti-HCV antibodies indicates prior exposure to HCV infection. However, the assay may be negative early in acute HCV infection, in immunosuppressed individuals. Anti-HCV antibodies can be detected by rapid immunoassays, enzyme-linked immunosorbent assays (ELISA), or chemiluminescent immunoassays. Quality of some of the test kits for anti-HCV may be a reason for concern.. Rapid immunoassay tests use a unique combination of modified HCV antigens conserved across all genotypes. However, some studies have demonstrated that rapid tests cannot be solely relied upon in screening patients from high- risk population in India. ELISA tests indicate presence of antibodies against HCV core, NS3 and NS5 regions. A study in North India has demonstrated the value of blood banks screening using two ELISA kits concurrently to improve detection of contaminated blood.. Enhanced chemiluminescence immunoassay (ECi) a quantitative assay based on chemiluminescent reaction, which is as good as ELISA.. ...
Our overall objective is to develop and standardize methodologies, accelerating the identification and development of non-invasive biomarkers as well as define new strategies to tackle major diseases such as cancer and parasitic diseases. Thus, REDiEX signifies a significant advance towards personalized medicine aiming to develop new tools and markers with high social impact that will generate economic benefits and equity in global health.
Group created with the aim of developing simple methods to identify biomarkers when preventive medicine requires them, for a proper diagnosis of a disease or the control of a patient.
bioMérieux announces the CE marking of 3 dengue immunoassays on VIDAS® Marcy lEtoile, France - April, 7th 2021... | April 20, 2021
Johnson & Johnson Vitros EciQ Immunoassay Analyzer, Enzyme for Sale. Check out our extensive catalog of New & Used Johnson & Johnson Vitros EciQ Immunoassay Analyzer, Enzyme devices. More than 200,000 items of Laboratory devices & medical equipment. 485634568
Excellent retention of beads, even after extensive washing. According to a publication at Journal of Immunological Methods by Prof. Beaumier at Baylor and Dr. Biswas at Merck, the DA-Bead method for analysis was comparable to, if not superior to, the conventional method in terms of consistency/precision, accuracy, sensitivity and dynamic range.. It is known that bead-based assays sometimes suffer from bead aggregation with plasma, blood, and other challenging biological samples which affects detectability of proteins and jeopardizes quality of data. Scientific studies have shown that DropArray may reduce bead aggregation and may better permit reading beads individually reporting the signals of target proteins as they are.. ...
Although there are extensive literature reports on the use of gold nanoparticle (AuNP) based homogeneous assays for detection of biomolecules, very few experimental description and procedures involving their preparation are described. In this study, AuNPs conjugated to Bovine Serum Albumin or Envelope protein from Dengue II were developed as a homogeneous immunoassay for antibody detection. We report here optimization of key parameters to prepare an immunoassay like conjugation protein concentration, centrifugation time, electrolyte addition and assay temperature. We determined that saturating protein concentrations improved AuNPs surface coverage and uniformity of the assay and addition of sodium chloride improved sensitivity of the antibody detection method and assay stability. Furthermore, we showed that dynamic light scattering can be used to monitor changes in gold nanoparticles in the preparation and detection steps. Additionally, numerical simulations of the plasmonic optical response of AuNPs
Sigma-Aldrich offers abstracts and full-text articles by [Matthew A Stiegel, Joachim D Pleil, Jon R Sobus, Marsha K Morgan, Michael C Madden].
One of the UKs leaders in the recruitment of science sales/commercial people at all levels from graduate to sales director. Our particular areas of interest are life sciences, biotechnology, molecular biology, chemistry, diagnostics, immunology and instrumentation. New jobs will be posted here as soon as we receive them along with news and information from Seltek, the life science / biotech markets and the recruitment industry in general.. ...
Biolan is a biotechnology company that works mainly in developing and marketing new biosensors capable of detecting health and agro-food-related molecules.
PubMed comprises more than 30 million citations for biomedical literature from MEDLINE, life science journals, and online books. Citations may include links to full-text content from PubMed Central and publisher web sites.
Federal contract opportunity W9113M-08-R-0010 for other measuring and controlling device manufacturing at Army Contracting Command U. S. Army Space and Missile Defense Command.
Multiplex immunoassays are an extremely powerful tool for protein detection and quantitation. Get a general overview and tips on how to optimize your results.
The New Quantitative Immunoassay Life Science Dashboard from Percepta Associates projects significant potential for switching primary immunoassay product suppliers
TY - JOUR. T1 - Simultaneous detection and quantification of six equine cytokines in plasma using a fluorescent microsphere immunoassay (FMIA). AU - Hall, SA. AU - Stucke, D. AU - Morrone, B. AU - Lebelt, D. AU - Zanella, AJ. N1 - 1023306. PY - 2015. Y1 - 2015. N2 - Cytokines are cell signalling proteins that mediate a number of different physiological responses. They are also biomarkers for inflammatory conditions and potential diagnostic references for diseases. Until recently, simultaneous quantification of cytokine profiles had not been possible. Now however, fluorescent microsphere immunoassays (FMIA) are able to measure multiple cytokines in a single sample. The following pro-inflammatory and anti inflammatory cytokines were quantified in equine plasma and serum samples: Interleukin (IL)-2, IL-4, IL-6, IL-10, Interferon (IFN)-γ, Tumor necrosis factor (TNF)-α.. AB - Cytokines are cell signalling proteins that mediate a number of different physiological responses. They are also biomarkers ...
Dry Fluorescence Immunoassay Analyzer Sales Market report provides a detailed evaluation of the market by highlighting information on different aspects which include drivers, restraints, opportunities, threats, and global markets including progress trends, competitive landscape analysis, and key regions expansion status. This report is comprehensive numerical analyses of the Dry Fluorescence Immunoassay Analyzer Sales industry and provides data for making strategies to increase the market growth and success. Dry Fluorescence Immunoassay Analyzer Sales Market finds essential elements of the this market in light of present industry, this market requests, business methodologies used by Dry Fluorescence Immunoassay Analyzer Sales Market players and the future prospects from different edges in detail.. Global Dry Fluorescence Immunoassay Analyzer Sales Market Report 2021-2026 contains systematic and point to point information regarding to Dry Fluorescence Immunoassay Analyzer Sales Industry and ...
EN] A lateral flow immunoassay (LFIA) was developed in the competitive reaction format and applied to test sulphathiazole (STZ) residues in honey samples. To prepare the assay test, a hapten conjugate and goat antirabbit antiserum as capture and control reagent, respectively, were dispensed on nitrocellulose membrane. Polyclonal antiserum against sulphathiazole was conjugated to colloidal gold nanoparticles and used as the detection reagent. The visual limit of detection (cut-off value) of the sulphathiazole LFIA was 15 ng/g, reaching qualitative results within 10 min. The assay was evaluated with STZ spiked honey samples from different geographical origins (n = 25). The results were in good agreement with those obtained from liquid chromatography separation and mass spectroscopy detection (LC-MS), indicating that the LFIA test might be used as a qualitative method for the determination of sulphathiazole residues without expensive equipment. The test was also highly specific, showing no ...
China Chemiluminescence Immunoassay,Fully Auto Chemiluminescence Immunoassay,Chemiluminescence Immune Assay,Clia Chemical Lumind Immunoassay, we offered that you can trust. Welcome to do business with us.
In this webinar, we share best practices to troubleshoot antibody conjugation to AnteoBind™ activated Estapor® europium microspheres in assay development and initial scale-up procedures.
Immunoassay Analyzer, Enzyme Manufacturer: Siemens Model: Immulite 2000 Condition: As is Year manufactured: 2005 (Estimated year) Location: Canada, Ontario Availability: Immediate Price: $5950.00. Seimens Immulite 2000, Immunoassay Analyzer - Hamilton - Medical equipment, supplies, Hamilton - 1430060
Myriad RBM has ultrasensitive immunoassays for all of these proteins from our CLIA-certified laboratory for viral pneumonia applications including COVID-19
North America Chemiluminescence Immunoassays (CLIA) Market provides pictorial methodology along with detailed sizing by capturing all dependent markets
Vitamin B12 is very important for the human body. Early diagnosis of vitamin B12 deficiency is essential because it is associated with many problems such as fatigue, lethargy, depression, poor memory, breathlessness, headaches, pale skin, mania, psychosis, etc. Most of the vitamin B12 molecules in serum bind to specific proteins as a complex; so, although researchers have developed some sensitive methods to quantify vitamin B12, few can be used for human serum detection of vitamin B12 because of disturbed releasing process. In this study, we are aiming to develop a rapid and accurate chemiluminescence immunoassay (CLIA) for human serum testing ...
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Chemiluminescence immunoassay clia kits for the laboratory. Famous supplier of clia kits reagent,You can get the clia test kits from Elabscience.
Looking for inhibition immunonephelometry? Find out information about inhibition immunonephelometry. A technique that uses a constant amount of antibody and a predetermined quantity of the same reactant as that assayed, chemically coupled to a... Explanation of inhibition immunonephelometry
Phadia (Pharmacia Diagnostics) Phadia 100 Immunoassay Analyzer, Enzyme for Sale. Check out our extensive catalog of New & Used Phadia (Pharmacia Diagnostics) Phadia 100 Immunoassay Analyzer, Enzyme devices. More than 200,000 items of Laboratory devices & medical equipment. 951075864
TY - JOUR. T1 - Performance characteristics of commercial immunoassays for the detection of IgG and IgM antibodies to b2 glycoprotein i and an initial assessment of newly developed reference materials for assay calibration. AU - Willis, Rohan. AU - Pierangeli, Silvia S.. AU - Jaskowski, Troy D.. AU - Malmberg, Elisabeth. AU - Guerra, Marta. AU - Salmon, Jane E.. AU - Petri, Michelle. AU - Branch, D. Ware. AU - Tebo, Anne E.. N1 - Funding Information: Corresponding author: Anne E. Tebo, PhD, 500 Chipeta Way, Salt Lake City, UT 84108; [email protected] Acknowledgments: M.P. is supported by grant from the National Institutes of Health (NIH) RO1 AR43727. M.G., J.S., M.P., and D.W.B. received grant support for the PROMISSE Study from the National Institutes of Health/National Institute of Arthritis and Musculoskeletal and Skin Diseases (NIH/NIAMS, RO1 AR49772). Anti-β2 glycoprotein I IgG and IgM kits were provided free of charge for this investigation by Bio-Rad, Corgenix, INOVA Diagnostics, ...
[107 Pages Report] Check for Discount on United States Automated Immunoassay Analyzers Market Report 2017 report by QYResearch Group. In this report, the United States Automated Immunoassay Analyzers market...
Optimize the patient workflow and improve patient care with Radiometers AQT90 FLEX immunoassay analyzer placed in emergency departments at the point of care
Optimize the patient workflow and improve patient care with Radiometers AQT90 FLEX immunoassay analyser placed in emergency departments at the point of care
A Luminex (Luminex Corp., Austin, TX) multiplex swine cytokine assay was developed to measure 8 cytokines simultaneously in pig serum for use in assessment of vaccine candidates. The fluorescent microsphere immunoassay (FMIA) was tested on archived sera in a porcine reproductive and respiratory syndrome virus (PRRSV) vaccine/challenge study. This FMIA simultaneously detects innate (IL-1β, IL-8, IFN-α, TNF-α, IL-12), regulatory (IL-10), Th1 (IFN-γ) and Th2 (IL-4) cytokines. These proteins were measured to evaluate serum cytokine levels associated with vaccination strategies that provided for different levels of protective immunity against PRRSV. Pigs were vaccinated with a modified-live virus (MLV) vaccine and subsequently challenged with a non-identical PRRSV isolate (93% identity in the glycoprotein (GP5) gene). Protection (as defined by no serum viremia) was observed in the MLV vaccinated pigs after PRRSV challenge but not those vaccinated with killed virus vaccine with adjuvant (KV/ADJ) (99%
TY - JOUR. T1 - Rapid and sensitive detection of 17β-estradiol in environmental water using automated immunoassay system with bacterial magnetic particles. AU - Tanaka, Tsuyoshi. AU - Takeda, Hajime. AU - Ueki, Fumiko. AU - Obata, Kimimichi. AU - Tajima, Hideji. AU - Takeyama, Haruko. AU - Goda, Yasuhiro. AU - Fujimoto, Shigeru. AU - Matsunaga, Tadashi. PY - 2004/3/4. Y1 - 2004/3/4. N2 - A fully automated immunoassay of 17β-estradiol (E2) was performed using anti-E2 monoclonal antibody immobilized on bacterial magnetic particles (AntiE2-BMPs) and alkaline phosphatase-conjugated E2 (ALP-E2). E2 concentration in environmental water samples was evaluated by decrease in luminescence based on competitive reaction. A linear correlation between the luminescence intensity and E2 concentration was obtained between 0.5 and 5ppb. The minimum detectable concentration of E2 was 20ppt. All measurement steps were done within 0.5h. The analysis of environmental water samples by a commercially available ELISA ...
Accurate ovulation prediction in pigs could lead to considerable reduction in losses incurred through failed artificial insemination, poorly controlled litter sizes and unnecessary culling of inaccurately diagnosed barren sows. The aim of this work is to predict the onset of oestrus in pigs by monitoring oestradiol levels in saliva. An automated method is proposed, for use outside the laboratory, based on single-use screen-printed electrochemical immunosensors. This approach follows on from previous work by the same authors towards an automated electrochemical progesterone monitor. This paper will describe the preparation of oestradiol sensors by immobilisation of monoclonal antibody onto the carbon electrode surface. Use of the sensors will be described in a competitive immunoassay format, where oestradiol (in buffer or saliva) competes against alkaline-phosphatase-labelled antigen for surface binding. Introduction of the enzyme substrate 1-naphthyl phosphate results in hydrolisis to produce ...
Streptococcus pneumoniae, Haemophilus influenzae, and Moraxella catarrhalis are pathogens commonly associated with infectious diseases in childhood. This study aimed to develop a fluorescent multiplexed bead-based immunoassay (FMIA) using recombinant proteins for the quantitation of serum IgG antibodies against these bacteria. Eight pneumococcal proteins (Ply, CbpA, PspA1, PspA2, PcpA, PhtD, SP1732-3 and SP2216-1), 3 proteins of H. influenzae (NTHi Protein D, NTHi0371-1, NTHi0830), and 5 proteins of M. catarrhalis (MC Omp CD, MC_RH4_2506, MC_RH4_1701, MC_RH4_3729-1, MC_RH4_4730) were used to develop the FMIA. Optimal coupling concentrations for each protein, comparison of singleplex and multiplex assays, specificity, reproducibility, and correlation to ELISA for six pneumococcal antigens were determined for validation. FMIA was then used to analyze acute and convalescent paired serum samples of 50 children with non-severe pneumonia. The coupling concentrations varied for different antigens, ...
results for this questionWhat is IgM/IgG rapid point of care?What is IgM/IgG rapid point of care?COVID-19 IgM/IgG Rapid Test BioMedomics has developed and launched one of the worlds first rapid point-of-care lateral flow immunoassays to aid in the diagnosis of coronavirus infection. The test detects both early marker and late marker, IgM/IgG antibodies in human finger-prick (capillary) or venous whole blood, serum, and plasma samples.COVID-19 IgM/IgG Rapid Test BioMedomics Inc.. ...
TY - JOUR. T1 - Production of monoclonal antibodies to ferritin and development of the enzyme immunoassay system. AU - Nozawa, S.. AU - Tsukazaki, K.. AU - Narisawa, S.. PY - 1985. Y1 - 1985. UR - http://www.scopus.com/inward/record.url?scp=0022353283&partnerID=8YFLogxK. UR - http://www.scopus.com/inward/citedby.url?scp=0022353283&partnerID=8YFLogxK. M3 - Article. C2 - 3910740. AN - SCOPUS:0022353283. VL - 37. SP - 2775. EP - 2783. JO - Nippon Sanka Fujinka Gakkai zasshi. JF - Nippon Sanka Fujinka Gakkai zasshi. SN - 0300-9165. IS - 12. ER - ...
An improved sandwich immunoassay for Toxoplasma gondii antigens associated with acute forms of Toxoplasma infection is disclosed. The assay employs either an F(ab)2 fragment of IgG which reacts specifically with such antigens or a monoclonal antibody for such antigens as the substrate layer in the sandwich. The assay is sensitive and, unlike prior sandwich assays for the antigen, is not affected significantly by cross-reactions with rheumatoid factor.
Emax ImmunoAssay Systems, Promega - Each : Dectect picogram levels of the neurotrophic and growth factors per milliliter of sample using these system
If you are a looking to purchase an immunoassay analyzer for your laboratory, or to replace an existing instrument, this guide provides important information to help you make the right decision.Learn about the different types of immunoassay technology, automation options, disease state assays and other important considerations.
Particle reagents, having polyether polyamine linking groups, for use in turbidimetric immunoassays are provided. These linking agents permit ready covalent attachment of compounds of biological interest to polymer particles and lead to immunoassays of improved precision.
As clinical diagnostic and surveillance tools, serological assays such as the enzyme-linked immunosorbent assay (ELISA) and the lateral flow immunoassay (LFIA) have been extensively used in the detection and identification of viruses and other pathogens. They achieve this aim through the use of highly specific recombinant antigens, or fusion proteins, to detect serum antibodies, which are used by the immune system to neutralize pathogenic bacteria, viruses or other microorganisms that cause disease. Serological assays have been successfully used in the detection of hepatitis viruses, the Ebola virus, HIV and influenza ...
Currently available enzyme immunoassay methods for peptides can be divided into two groups, homogeneous and heterogeneous methods. Homogeneous enzyme immunoassay methods, in which signals that are directly obtained from a mixture of test samples and reagents correlate with the amount of peptide in test samples, are simpler and quicker but less sensitive than heterogeneous enzyme immunoassay methods, in which free and bound forms of enzyme-labeled reactants are separated from each other.
Product availability varies by country.. * Under development. Not available for sale.. † Not available for sale in the U.S.. ‡ ADVIA Centaur HCV and HIV assays are developed, manufactured, and sold by Siemens for Ortho-Clinical Diagnostics, Inc. and Grifols Diagnostic Solutions Inc.. § Not CE marked. The products/features (mentioned herein) are not commercially available in all countries. Due to regulatory reasons their future availability cannot be guaranteed. Please contact your local Siemens organization for further details. ...
Product availability varies by country.. * Under development. Not available for sale.. † Not available for sale in the U.S.. ‡ The ADVIA Centaur HCV and HIV assays are developed, manufactured and sold by Siemens for Ortho-Clinical Diagnostics, Inc. and Grifols Diagnostic Solutions Inc.. § Not CE marked. The products/features (mentioned herein) are not commercially available in all countries. Due to regulatory reasons their future availability cannot be guaranteed. Please contact your local Siemens organization for further details. ...
The CDIA™ Olaquindox Metabolite Colloidal Gold Test Cassette (Meat) Is A Lateral Flow Immunoassay For The Detection Of Olaquindox Metabolite In Meat.
TY - JOUR. T1 - Comparison of different cyclosporine immunoassays to monitor C0 and C2 blood levels from kidney transplant recipients. T2 - Not simply overestimation. AU - Cattaneo, Dario. AU - Zenoni, Stefania. AU - Murgia, Stefano. AU - Merlini, Simona. AU - Baldelli, Sara. AU - Perico, Norberto. AU - Gotti, Eliana. AU - Ottomano, Cosimo. AU - Crippa, Alberto. AU - Remuzzi, Giuseppe. PY - 2005/5. Y1 - 2005/5. N2 - Background: Immunoassays used for the measurement of cyclosporine (CsA) usually show cross-reactivity for CsA metabolites, usually resulting in unacceptable bias. Methods: To assess the performance of different immunoassays, CsA concentrations were analyzed in 132 samples using ACMIA, EMIT-VIVA, CEDIA-PLUS, and HPLC. Samples were collected from kidney transplant patients monitored with the traditional blood CsA trough level (C0, n=73) and the new sampling at 2-h post CsA dosing (C2, n=59). Results: Overall, the correlations between HPLC and other methods were good (r values ranging ...
Product availability varies by country.. * Under development. Not available for sale.. † Not available for sale in the U.S.. ‡ The ADVIA Centaur HCV and HIV assays are developed, manufactured and sold by Siemens for Ortho-Clinical Diagnostics, Inc. and Grifols Diagnostic Solutions Inc.. § Not CE marked ...
The CDIA™ Erythrosin Colloidal Gold Test Cassette (Soft Drinks) Is A Lateral Flow Immunoassay For The Detection Of Erythrosin In Soft Drinks.
Product Description specificalThe micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human CGalpha. Standards or samples are added to the appropriate micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human CGalpha and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human CGalpha, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit(RLU) value is measured spectrophotometrically by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human CGalpha. You can calculate the concentration of Human CGalpha in the samples by comparing the RLU value of the samples to the standard curve. ...
The problem is particularly prominent in developing countries, where infectious diseases often go undetected for long periods of time, resulting in widespread epidemics.. Currently, cheap point-of-care methods employed to detect infectious diseases have their limitations, particularly when it comes to detecting very low levels of markers in the blood.. Now, Imperial researchers, alongside their collaborators at University College London, have developed a new diagnostic tool for HIV that is affordable, capable of withstanding harsh conditions and highly sensitive, making the early detection of the disease possible.. The research, published in the journal ACS Nano, refined a current diagnostic method known in the field as a lateral flow immunoassay, a similar method employed by pregnancy tests.. In the case of HIV, if a patient is positive, virus components present in the blood will bind to coloured particles on the paper test, indicating HIV infection.. To the best of our knowledge, our newly ...
The ALUXBIO product portfolio encompasses Drugs of Abuse test , Womens Health test, Infectious Disease test ,Tumor Marker test and Cardiac Markers Test.The majority of products utilize lateral flow immunoassay technology. More than 100 specifications are available. Our manufacturing facility is ISO 13485:2003 certified, and most of our products are CE marked.
Immunoassay analyzer in work processing,analysis and diagnostics for ,allergy,anemia,cardiac,diabetes,growth ,hormone,hepatitis,Inflammation,tumor markers and the other microbiology parameters ...
AIA-360 Automated Immunoassay Analyzers size and affordability make it an excellent fit for POLs and small hospitals,as well as for specialty testing or for use as a back-up analyzer.
The report forecast global Automated Immunoassay Analyzers market to grow to reach xxx Million USD in 2019 with a CAGR of xx% during the period 2020-2024...
Pyrimethanil is a widely used post-harvest fungicide whose residues are commonly found in fruits and vegetables. We herein report the synthesis of two new functionalized derivatives of pyrimethanil carrying an equivalent spacer arm at different positions of the molecule. The influence of the linker tethering site on the affinity of monoclonal antibodies was shown. Moreover, the development of competitive immunoassays in different formats and with high sensitivity to the target analyte - IC,inf,50,/inf, values below 0.3μg/L - is described. Optimized assays were characterized by the determination of the limit of quantification, trueness, and precision using water-diluted QuEChERS extracts of fruits and vegetables fortified with pyrimethanil. Finally, in-field treated and blind spiked strawberry, tomato, and cucumber samples were analyzed by the developed immunoassays and a reference chromatographic method. Using Deming regression analysis, we show that results from both methodologies were ...
Reveal for Histamine is a single-step lateral flow assay based on a competitive immunoassay format. The extract is wicked through a reagent zone, which contains histamine-specific antibodies conjugated to coloured particles. If histamine is present, it will be captured by the particle-antibody complex. The histamine-antibody-particle complex then is wicked onto a membrane, which contains a zone of histamine conjugated to a protein carrier. This zone captures only uncomplexed antibody-particle conjugate, allowing the coloured particles to concentrate and form a visible line. The membrane also contains a control zone where the antibody-particle complex present in the reagent zone is captured by an antibody, forming a visible line. The control line will always form regardless of the presence of histamine, ensuring the strip is functioning properly. In addition, the control line also is used as a reference for comparison to the test zone for visual interpretation of the test results. If histamine is ...
Electrochemiluminescence immunoassay (ECLIA) for the in vitro quantitative determination of tacrolimus in human whole blood IndicationThe Elecsys Tacrolimus assay is used as an aid in the management of heart, liver and kidney transplant patients receiving tacrolimus therapy. Organ transplant patients are prescribed immunosuppressant drugs (ISD) like e.g. tacrolimus so that the immune system does not reject the newly transplanted organ. ISD assays are used to determine the drug concentration in the patients blood as a guide for effective and well-tolerated doses as the desired therapeutic effect is only obtained in a narrow therapeutic range: The dose must be high enough to prevent organ rejection, and the dose must be low enough to avoid drug toxicity and opportunistic infections ...
China Medical Technologies, Inc., a medical device company, develops, manufactures, and markets immunodiagnostic and molecular diagnostic products. It uses molecular diagnostic technologies, including fluorescent in situ hybridization (FISH) and surface plasmon resonance (SPR), and enhanced chemiluminescence immunoassay (ECLIA), an immunodiagnostic technology in the development, manufacturing, and distribution of diagnostic products for the detection of various cancers, diseases, and disorders, as well as companion diagnostic tests for targeted cancer drugs. The company offers immunodiagnostic products, such as ECLIA analyzers and reagent kits for various clinical applications, including anemia, cardiac diseases, food safety, growth disorders, hepatitis, HIV, infertility, liver fibrosis, metabolic function, reproductive endocrinology, SARS, thyroid disorders, and tumors tests. It also provides FISH probes, a molecular diagnostic IVD reagent for the prenatal diagnosis of various genetic diseases; ...
PubMed comprises more than 30 million citations for biomedical literature from MEDLINE, life science journals, and online books. Citations may include links to full-text content from PubMed Central and publisher web sites.
In the report the esoteric testing market, the market is expected to reach US$ $ 45,081.6 Mn in 2025 from US$ 19,868.2 in 2017. The market is estimated to grow with a CAGR of 11.7% from 2018-2025. The esoteric testing market is driven by the driving...
Interpretation of hormone levels in older patients: points for consideration. (Sztefko and Szybowska 2012) Download. Blood hormone and tumor marker concentrations are usually determined by immunochemical methods which are based on an unique reaction between antigen and assay capture antibody. Despite the speed and simplicity of assays performance on automatic immunochemistry platforms, the interpretation of final results requires a deep knowledge of method fallibility. General lack of immunoassays standardization, presence of cross-reacting substances in patients sample, limitation of free hormones measurement due to abnormal analyte binding protein concentrations, assay interferences due to patients autoantibodies, and heterophilic antibodies, as well as proper interpretation of very low- and very high-sample analyte levels, are the main points discussed in respect to hormones and tumor markers measurement in geriatric population.. Laboratory values in fit aging individuals--sexagenarians ...
A method and apparatus for performing a chemiluminescent immunoassay featuring electrochemical techniques to generate an oxidant used to trigger the chemiluminescence of the labelled immunoreactant. The generation of the oxidant is precisely controlled and the oxidant precisely and uniformly delivered to provide a more accurate, precise and sensitive assay.
Vaccines in vitro characterization is required to identify optimal formulation conditions to ensure physical, chemical, and biological integrity of antigens and adjuvants. Analytical methods currently in use are mostly suitable for the characterization of unformulated antigens - requiring the complete desorption from aluminum-based adjuvants - and are not always able to reveal individual antigens in vaccine combinations. Here, the Luminex technology is proposed as proof of concept for the development of an improved analytical method for vaccine characterization, based on the use of specific antibodies bound to magnetic microspheres presenting unique digital signatures for simultaneous recognition of respective antigens in the entire formulation. TdaP combination vaccines were chosen as a model to develop an assay able to quantify acellular pertussis antigens and their levels of adsorption to adjuvant at the same time. This assay was directly applicable on the commercial vaccine product, avoiding ...
SERS-based immunoassay[edit]. SERS-based immunoassay is a novel technique for detection of low abundance biomarkers. Using ... Early detection of pancreatic cancer biomarkers was accomplished using SERS-based immunoassay approach.[43] This technology has ... "Multiplex detection of pancreatic cancer biomarkers using a SERS-based immunoassay". IOP Nanotechnology. Bibcode:2017Nanot.. ...
The unit count will often still remain in use.[clarify] Such a change has happened with the immunoassay standards for prolactin ... Ekins R (1991). "Immunoassay standardization". Scand J Clin Lab Invest Suppl. 205: 33-46. doi:10.3109/00365519109104600. PMID ... Wicher JT (1991). "Calibration is the key to immunoassay but the ideal calibrator is unattainable". Scand J Clin Lab Invest ...
That Tjien Ngo (1988). Nonisotopic immunoassay. Plenum Press. p. 71. F. Yamagishi; T. Stanford; C. van Ast (2001). "Biosensors ...
Immunoassay. *Boronate affinity chromatography. In the United States, HbA1c testing laboratories are certified by the National ...
An important practical relevance of the phenomenon is as a type of interference that plagues certain immunoassays and ... Tate, Jill; Ward, Greg (2004-05-01). "Interferences in Immunoassay". The Clinical Biochemist Reviews. 25 (2): 105-120. ISSN ... Miller, James (April 2004). "Interference in immunoassays: avoiding erroneous results" (PDF). CLI Online. CLI. Retrieved 3 ... Blocking antibody Schiettecatte, Johan; Anckaert, Ellen; Smitz, Johan (2012-03-23). "Interferences in Immunoassays". Advances ...
In the heterogeneous immunoassay the separation step will wash away the free substrate unable to bind due to HAMA blocking; ... Darwish, IA (2006). "Immunoassay Methods and their Applications in Pharmaceutical Analysis: Basic Methodology and Recent ... Tate, J; Ward, G (2004). "Interferences in immunoassay". Clin Biochem Rev. 25 (2): 105-20. PMC 1904417. PMID 18458713.. ... HAMA interferences can give false positive or negative immunoassay results. HAMA bridging interference produces artificially ...
Sarah Tiplady (2013). "7.3: Lateral Flow and Consumer Diagnostics". The Immunoassay Handbook. pp. 533-536. Retrieved 2013-10-17 ... "Chapter 7.4: Consumer Diagnostics-Clearblue Pregnancy and Fertility Testing". The Immunoassay Handbook. pp. 537-540. Retrieved ...
Kricka, Larry J (1993). "Ultrasensitive immunoassay techniques". Clinical Biochemistry. 26 (5): 325-331. doi:10.1016/0009-9120( ...
Furthermore, immunoassays on thiol-ene "synthetic paper" micropillar scaffolds have shown to generate a superior fluorescence ... Guo, W; Vilaplana, L; Hansson, J; Marco, P; van der Wijngaart, W (2020). "Immunoassays on thiol-ene synthetic paper generate a ... Silzel J. W., Cercek B., Dodson C., Tsay T., Obremski R. J. (1998). "Mass-sensing, multianalyte microarray immunoassay with ... Ekins RP (1989). "Multi-analyte immunoassay". J Pharm Biomed Anal. 7 (2): 155-68. doi:10.1016/0731-7085(89)80079-2. PMID ...
A new avidin-biotin ELISA assay for its measurement". Journal of Immunoassay. 13 (1): 47-60. doi:10.1080/15321819208019824. ... using immunoassays such as nephelometry or particle-enhanced turbidimetry. It is a more expensive test than serum creatinine ( ... "Adult cystatin C reference intervals determined by nephelometric immunoassay". Clinical Biochemistry. 40 (13-14): 1084-7. doi: ...
Mills E, Potts A, Plumb G, Lambert N, Morgan M (1997). "Development of a rapid dipstick immunoassay for the detection of peanut ... In these tests, peanut allergens are measured in foods by immunoassay with human IgE antibodies. In 2003 a monoclonal antibody- ... Keating MU, Jones RT, Worley NJ, Shively CA, Yunginger JW (July 1990). "Immunoassay of peanut allergens in food-processing ... Yeung JM, Collins PG (1996). "Enzyme immunoassay for determination of peanut proteins in food products". Journal of AOAC ...
Examples of these methods are the MSIA (mass spectrometric immunoassay), developed by Randall Nelson in 1995, and the SISCAPA ( ... 1995). "Mass Spectrometric Immunoassay". Analytical Chemistry. 67 (7): 1153-1158. doi:10.1021/ac00103a003. ISSN 0003-2700. PMID ... Generally, proteins may be detected by using either antibodies (immunoassays) or mass spectrometry. If a complex biological ... The lower limit of detection with conventional immunoassay technology is the upper femtomolar range (10−13 M). Digital ...
... and immunoassay test kits. New field analytical methods are fast developing. However, some have used standard laboratory ... Immunoassay and Enzymatic Assays". "Technologies for Cleaning up Contaminated Sites" (PDF). 2015-01-27. http://www.triadcentral ...
Method: Microparticle Enzyme Immunoassay. Beckman Coulter provides the equipment and reagents used in the 2009-2011 CDC manuals ... Method: Microparticle Enzyme Immunoassay. Lab 18 TSH - Thyroid Stimulating Hormone. 1999-2000. ...
Immunoassay of human aldose reductase". Biochemical Pharmacology. 46 (1): 21-8. doi:10.1016/0006-2952(93)90343-U. PMID 8347133 ...
Both heterogeneous immunoassays (antigens interacting with immobilized antibodies) and homogeneous immunoassays (antigens ... have been incorporated onto DMF immunoassay platforms. The incorporation of magnetic bead-based assays onto a DMF immunoassay ... Shamsi MH, Choi K, Ng AH, Wheeler AR (February 2014). "A digital microfluidic electrochemical immunoassay". Lab on a Chip. 14 ( ... The advanced fluid handling capabilities of digital microfluidics (DMF) allows for the adoption of DMF as an immunoassay ...
Assay Immunoassay Hoskins, W. M.; Craig, R. (1962-01-01). "Uses of Bioassay in Entomology". Annual Review of Entomology. 7 (1 ...
September 2017). "Immunoassay for human serum erythroferrone". Blood. 130 (10): 1243-1246. doi:10.1182/blood-2017-04-777987. ... Erythroferrone levels in blood have been shown by immunoassay to be higher after blood loss or erythropoetin administration. ...
... fluorescent immunoassay products (Sofia); and (5) molecular diagnostic products. These products include assays such as QuickVue ... Quidel's core competencies and capabilities include immunoassay development, automated manufacturing, monoclonal antibody ...
Shamsi MH, Choi K, Ng AH, Wheeler AR (February 2014). "A digital microfluidic electrochemical immunoassay". Lab on a Chip. 14 ( ... magnetic microparticles in the fluids replaced the typical fluorescence-based detection of droplets in the immunoassay of ...
The photoacoustic immunoassay labels and detects target proteins using nanoparticles that can generate strong acoustic signals ... Zhao Y, Cao M, McClelland JF, Lu M (2016). "A photoacoustic immunoassay for biomarker detection". Biosensors and Bioelectronics ...
Fluorescence polarization immunoassay is widely used. This test is slower and has a lower sensitivity than digoxin immunoassay ... and their detection by digoxin immunoassays". Clinical Chemistry. 42 (10): 1654-8. doi:10.1093/clinchem/42.10.1654. PMID ...
Another identification mechanism is through immunoassay. Abbott Labs' AxSYM is an immunoassay device utilizing Fluorescence ... Polarization Immunoassay (FPIA) technology that can determine the presence and quantify salicylates. The introduction of a ...
Use of enzymes in immunoassay techniques. Analyst, 109: 533-547. Gugerli, P. and Gehriger, W. (1980). Enzyme-linked ... Laboratory techniques in biochemistry and molecular biology practice and theory of enzyme immunoassays, volume 15, Elsevier ...
The photoacoustic immunoassay labels and detects target proteins using nanoparticles that can generate strong acoustic signals ... "A photoacoustic immunoassay for biomarker detection". Biosensors and Bioelectronics. 85: 261-66. doi:10.1016/j.bios.2016.05.028 ...
Surround optical-fiber immunoassay (SOFIA). *XENPAK. References[edit]. *^ "Optical Fiber". www.thefoa.org. The Fiber Optic ...
In the 1970s, the discovery of monoclonal antibodies led to the development of the relatively simple and cheap immunoassays, ... Yalow RS, Berson SA (July 1960). "Immunoassay of endogenous plasma insulin in man". Journal of Clinical Investigation. 39 (7): ...
2007 Dec 12;605(1):70-9. Nelson R, Krone J, Bieber A. Mass spectrometric immunoassay. Anal Chem. 1995 Ackermann BL. Hybrid ... Serum thyroglobulin: a model of immunoassay imperfection. 2006;(8):12-4. Hoofnagle AN, Roth MY. Improving the Measurement of ... "mass spectrometric immunoassay",) in which antibodies are used to enrich target proteins, which are analyzed intact by MS; and ... including conventional immunoassays (e.g., ELISA assays). Elimination of such autoantibody interferences drove the adoption of ...
Evaluation of the Abbott AxSYM Immunoassay Analyser - Agnes D'Souza, M. J. Wheeler, Great Britain. Medical Devices Agency (6S)- ... Smith, J; Osikowicz, G (October 1993). "Abbott AxSYM random and continuous access immunoassay system for improved workflow in ... Evaluation of the Abbott AxSYM immunoassay analyser. Medical Devices Agency. 1996. ISBN 9781858395388. ...
Yalow, RS; Berson, SA (1960). "Immunoassay of endogenous plasma insulin in man". J Clin Invest. 39 (7): 1157-75. doi:10.1172/ ...
Mass spectrometric immunoassay (MSIA) is a method that uses antibodies to analyze protein differences in biological samples.[1] ... 1995). "Mass Spectrometric Immunoassay". Analytical Chemistry. 67 (7): 1153-1158. doi:10.1021/ac00103a003. ISSN 0003-2700. PMID ... Retrieved from "https://en.wikipedia.org/w/index.php?title=Mass_spectrometric_immunoassay&oldid=758985804" ...
Definition Immunoassays are chemical tests used to detect or quantify a specific substance, the analyte, in a blood or body ... Law, B. Immunoassay: A Practical Guide. London: Taylor and Francis, 1996.. Wild, D., ed. Immunoassay Handbook, 2nd Ed. London: ... Chemiluminescent immunoassay- An immunoassay in which the label is a molecule that emits light when excited by a chemical ... Enzyme immunoassay- An immunoassay using an enzyme as the label. The enzymatic reaction product is measured to determine the ...
Immunoassays are chemical tests used to detect or quantify a specific substance, the analyse, in a blood or body fluid sample, ...
Migratory detection immunoassay. US4978502 *. Dec 15, 1987. Dec 18, 1990. Dole Associates, Inc.. Immunoassay or diagnostic ... Three Layered Dry Phase Immunoassay Device. A light emitting immunoassay device in accordance with the present invention was ... The immunoassay devices are preferably held or positioned by use of a holding assembly such as a test strip holder known in the ... Multi-spot enzyme immunoassay method. US4818677 *. Dec 3, 1987. Apr 4, 1989. Monoclonal Antibodies, Inc.. Membrane assay using ...
A thin layer of constant thickness of a liquid specimen for immunoassay is thus receivable between the complementary surfaces. ... Apparatus for performing an immunoassay, comprises in combination a reaction tube closed at its bottom, and in the reaction ... A further object of the present invention is to provide an element for immunoassay which can perform immunoassay with a small ... Solid phase immunoassay with carriers matching the shape of sample wells. US6207463. 20 Oct 1995. 27 Mar 2001. Labsystems Oy. ...
horseradish peroxidase and b-galatosidase.Enzyme Immunoassay (EIA) • In enzyme immunoassays (EIA). ... Immunoassays that require separation of the bound Ab-Ag* complex are referred to as being heterogeneous immunoassays. • ... Those that do not require separation are referred to as homogeneous immunoassays.Heterogeneous and Homogeneous Immunoassays ... Immunoassays use one or more select antibodies to detect analytes of interest. ...
Immunoassays are analytical techniques that are widely used to detect and quantitate the concentration of a protein, metabolite ... Immunoassays are analytical techniques that are widely used to detect and quantitate the concentration of a protein, metabolite ... Homogeneous immunoassays do not require a physical separation step.. *Antigens present in excess concentrations can produce ... Immunoassays are also the mainstays in research, for example, understanding the pathophysiology of medical disorders in order ...
Membrane-based immunoassay method. US5432057 *. 28 Aug 1992. 11 Jul 1995. Syva Company. Simultaneous calibration heterogeneous ... Simultaneous calibration heterogeneous immunoassay. US5354692 *. 8 Sep 1992. 11 Oct 1994. Pacific Biotech, Inc.. Analyte ... Simultaneous calibration heterogeneous immunoassay. US5308775 *. 15 Sep 1992. 3 May 1994. Abbott Laboratories. Assay devices ... Simultaneous calibration heterogeneous immunoassay. US4540659 *. 16 Jul 1982. 10 Sep 1985. Syva Company. Simultaneous ...
Immunoassay using magnetic particle. EP0420186A3 *. Sep 26, 1990. Jul 31, 1991. Fujirebio Inc.. Immunoassay using magnetic ... Metal sol particle immunoassay. US4342739 *. Jan 8, 1980. Aug 3, 1982. Fuji Photo Film Co., Ltd.. Novel material for ... Laser magnetic immunoassay method and method by a magnetophoresis apparatus therefor. US5238811 *. Apr 27, 1992. Aug 24, 1993. ... A method of immunoassay comprising the steps of reacting an antigen with an antibody, one of which is the biologically active ...
Quantitative Measurement of Active Neutrophil Elastase Levels Using ProteaseTag™ Active NE Immunoassay. *Download PDF Copy ... Active NE is captured using the NE-Tag in the ProteaseTag™ Active NE Immunoassay (Figure 1). A following antibody step allows ... ProteaseTag™ Active Neutrophil Elastase Immunoassay (PA001) from ProAxsis. Before starting the assay, samples of sputum were ... On binding, they bridge a solid support, facilitating their incorporation into a format of immunoassay for particular ...
... described according to their kinetics and structure only a few have turned out to be marker enzymes in the enzyme immunoassay ( ... Porstmann B., Porstmann T. (1988) Chromogenic Substrates for Enzyme Immunoassay. In: Ngo T.T. (eds) Nonisotopic Immunoassay. ... 5-tetramethylbenzidine as an Ames test negative chromogen for horse-radish peroxidase in enzyme-immunoassay, J. of Immunoassay ... Cox, C., 1983, NAD labeled immunoassays, Trends in Anal. Chem., 2: 129-131.CrossRefGoogle Scholar ...
... Leading medical CMO to provide high-precision, ... is proud to announce the opening of the companys first lateral flow immunoassay (LFI) production facility. Co-located within ...
Immunoassay Cellular System is a no-wash bioluminescent immunoassay that measures levels of target analytes in cell lysates ... Lumit™ Immunoassay vs. Western Blot Lumit™ Immunoassays are easier and much faster than traditional Western blots while ... The Lumit™ Immunoassay Cellular System is a no-wash bioluminescent immunoassay that measures target analytes directly in cell ... How the Lumit™ Immunoassay Cellular System Works Lumit™ Immunoassay Cellular Systems include Lumit™ Secondary Antibodies ( ...
Worldwide, IMMULITE systems have been chosen for various types of immunoassay testing due to their flexible platforms and ... The IMMULITE chemiluminescent immunoassay system is designed for ease of use, reliability, high-performance assays and fully ... Access Immunoassay System. 8. Access 2 Immunoassay System. 9. ADVIA Centaur Immunoassay System. 10. AIA - 600 II Immunoassay ... Immulite 1000 Immunoassay System. 2. Immulite 2000 Advanced Immunoassay System. 3. Immulite 2500 SMS. 4. Immulite PAP. 5. ...
R&D Systems™ Quantikine™ Immunoassay Control Set 919 for Human Total IL-18. Immunoassay control set for Human Interleukin 18 ... R&D Systems™ Quantikine Immunoassay Control Group 10 Control for Human High Sensitivity IL-1 beta, IL-4, IL-7, IL-8, IL-10, IL- ... R&D Systems™ Quantikine Immunoassay Control Group 8 Control for Human CXCL6/GCP-2, CXCL8/IL-8, CXCL10/IP-10, CXCL11/I-TAC, ... R&D Systems™ Quantikine Immunoassay Control Group 9 Control for Human AgRP, Carbonic Anhydrase IX, CD23/Fc epsilon RII, FGF-19 ...
... ,ARUP Laboratories is a national reference laboratory and a worldwide leader ... Access Immunoassay System. 4. Access 2 Immunoassay System. 5. ADVIA Centaur Immunoassay System. 6. AIA - 600 II Immunoassay ... AIA - 360 Immunoassay Analyzer. 8. AIA - 1800 Immunoassay Analyzer. 9. Immulite Chemiluminescent Immunoassay System. 10. ...
Using the advantages of both instrumental analysis and immunoassay, we support you to construct an advanced quality control ... SmartAssay test kits are complete immunoassays for analyzing pesticide residue, including all required reagents. These kits ...
Multi-step assays are often called separation immunoassays or heterogeneous immunoassays. Some immunoassays can be carried out ... Immunoassays can be run in a number of different formats. Generally, an immunoassay will fall into one of several categories ... Immunoassays come in many different formats and variations. Immunoassays may be run in multiple steps with reagents being added ... "The Immunoassay Handbook", 3rd Edition, David Wild, Ed., Elsevier,2008 Immunoassay at the US National Library of Medicine ...
... method to efficiently express high levels of a recombinant untagged NT-proBNP for use as a calibrator in NT-proBNP immunoassays ... In such immunoassays, the purified analyte is also the calibration material. (see The Immunoassay Handbook, edited by David ... protein is capable of functioning as a calibrator in immunoassays specific for NT-proBNP such as those immunoassays disclosed ... 3. The purified NT-BNP of claim 2 capable of functioning as a calibrator in an immunoassay specific for NT-BNP. 4. A series of ...
Some of the major factors to consider when designing a highly parallel multiplex immunoassay are variability, sample collection ... Variability between immunoassay kits. Multiplex immunoassay kits, even from the same manufacturer, may not give identical ... Multiplex immunoassays and enzyme-linked immunosorbent assays (ELISA) are used to determine and analyze the presence of an ... Multiplex immunoassays can be differentiated by the surface to which the antigens or antibodies are attached. These surfaces ...
The immunoassay of claim 9, wherein the sample is taken from a human. 12. The immunoassay of claim 11, wherein the antibody is ... 2. The immunoassay of claim 1, wherein the sample is a human sample. 3. The immunoassay of claim 2, wherein the antibody is ... 4. The immunoassay of claim 3, wherein the antibody is attached to a solid support. 5. The immunoassay of claim 4, wherein the ... 8. The immunoassay of claim 7, wherein R7 is 2,4-dinitrophenyl. 9. The immunoassay of claim 3, wherein the protecting group is ...
Switching to an automated immunoassay platform. Switching from an ELISA format to Gyrolab immunoassay technology should not ... Home Magazine BioPerspectives Automating and Miniaturizing Immunoassays: Continuing the Progress to Maximum Productivity ... Transferring immunoassays from traditional standard formats such as ELISA onto an automated platform like Gyrolab offers ... Miniaturizing immunoassays can deliver tremendous savings in sample volumes, animal use, and reagent consumption. Although ...
An enzyme immunoassay is any of several immunoassay methods that use an enzyme bound to an antigen or antibody. These may ... include: Enzyme-linked immunosorbent assay (ELISA) Enzyme multiplied immunoassay technique (EMIT) This article includes a list ...
An improved microscale diffusion immunoassay utilizing multivalent reactants is disclosed. In particular, a method for ... Microscale diffusion immunoassay. US20020090644. 14 Feb 2000. 11 Jul 2002. Weigl Bernhard H.. Microscale diffusion immunoassay ... The present invention relates to immunoassays, and, more particularly, to improved microscale diffusion immunoassays utilizing ... Flow injection immunoassays have taken advantage of specific flow conditions (see U. de Alwis and G. S. Wilson, Anal. Chem. 59 ...
Browse 352 market data tables and 40 figures spread through 305 pages and in-depth TOC on "Immunoassay Market" ... This segment is also expected to grow at the highest CAGR during the forecast period, owing to the increase in immunoassay ... Based on region, the global immunoassay market is dominated by North America, followed by Europe, Asia, and the Rest of the ... In Vitro Diagnostic (IVD) Market [Instruments, Reagents & Data Management Systems] [Technique (Immunoassay, Clinical Chemistry ...
Immunoassay and Other Bioanalytical Techniques describes the strength and the versatility of these methods in a wide range of ... including multiplexed immunoassays. It presents standard and innovative applications of immunochemical and related methods, ...
The present invention is directed to a membrane-based immunoassay method for an analyte of interest having at least two ... Immunoassay, 13 127-131 (1990).. 4. Bangs, L., "New Developments In Particle-Based Tests and Immunoassays," J. Int. Fed. Clin. ... Immunoassay, 13 127 131 (1990).. 8. *. Bangs, L., New Developments In Particle Based Tests and Immunoassays, J. Int. Fed. Clin ... "Latex Immunoassays," J. Clin. Immunoassay, 13 127-131 (1980) both of which are incorporated herein by reference. Various ...
Immunoassays are presently used worldwide for the rapid screening of drugs. Despite the fact that they are a highly valuable ... Schütz H. Drogenscreening mit Immunoassays. Pharmazie in unserer Zeit 1999;28:320-328.PubMedCrossRefGoogle Scholar ... Immunoassays are presently used worldwide for the rapid screening of drugs. Despite the fact that they are a highly valuable ... Effect of glutaraldehyde (Urin Aid) on detection of abused drugs in urine by immunoassay. Clin Chem 1994;40:1605, 1606.PubMed ...
... applications and companies active in the industry , Biokit, Carolina Liquid ... Analysis of Automated Immunoassay Analyzers Market applications and companies active in the industry , Biokit, Carolina Liquid ... The emergence of automation in the medical sector is one of the many drivers that will fuel automated immunoassay analyzers ... India is one of those regions that is likely to observe exponential growth in automated immunoassay analyzers market. In India ...
See also changes related to Immunoassay, or pages that link to Immunoassay or to this page or whose text contains "Immunoassay" ... Immunosorbent technique [r]: Sensitive immunoassay that uses an enzyme linked to an antibody or antigen as a marker for the ... Retrieved from "http://en.citizendium.org/wiki?title=Immunoassay/Related_Articles&oldid=100394252" ...
  • An example of a qualitative assay is an immunoassay test for pregnancy. (encyclopedia.com)
  • The purpose of an immunoassay is to measure (or, in a qualitative assay, to detect) an analyte. (encyclopedia.com)
  • The molecule detected by the immunoassay is often referred to as an "analyte" and is in many cases a protein, although it may be other kinds of molecules, of different sizes and types, as long as the proper antibodies that have the required properties for the assay are developed. (wikipedia.org)
  • Immunoassays may be run in multiple steps with reagents being added and washed away or separated at different points in the assay. (wikipedia.org)
  • Enzyme Linked Immunosorbent Assay (ELISA) is an application of solid phase heterogeneous sandwich immunoassay that combines enzyme-antibody label reagent with a solid phase bound antibody. (scribd.com)
  • These may include: Enzyme-linked immunosorbent assay (ELISA) Enzyme multiplied immunoassay technique (EMIT) This article includes a list of related items that share the same name (or similar names). (wikipedia.org)
  • It shows (a) how miniaturizing immunoassays can deliver significant savings in sample volumes and (b) how the Gyrolab platform secures smooth assay/method transfer between key entities such as pharma companies, CROs, and CMOs, even those on different continents. (genengnews.com)
  • Although reducing assay volumes in manual ELISA immunoassays is possible, it is tedious to carry out and challenging to maintain assay performance. (genengnews.com)
  • ELISA method development and the design of our assay validations are tailored to meet both client and regulatory requirements, and we are experienced in working with clients on the generation and qualification of critical immunoassay reagents. (criver.com)
  • On the basis of technique the market is segmented into enzyme-linked immunosorbent assay (ELISA), Radioimmunoassay (RIA), chemiluminescence immunoassays (CLIA) and others. (openpr.com)
  • Diagnostic accuracy of the immunoassay was assessed against a whole spike protein-based recombinant immunofluorescence assay (rIFA) by receiver operating characteristic (ROC) analyses. (nih.gov)
  • To find the best assay for your needs, choose from popular immunoassay categories below or contact our field application specialists (FAS) , who can answer your questions and provide you with individualized immunoassay support, including information about new immunoassay technologies. (thermofisher.com)
  • All our immunoassay systems feature standardized test menus, assay protocols, instrument processes and reference ranges. (beckmancoulter.com)
  • During development, an immunoassay, including components and individual assay steps, goes through many levels of screening to ensure that it accurately and reproducibly measures the target protein of interest, with stringent quality control measures to ensure consistency over time. (rndsystems.com)
  • the most common types are enzyme-linked immunosorbent assay (ELISA) and enzyme-multiplied immunoassay technique (EMIT). (thefreedictionary.com)
  • Enzyme immunoassay (EIA) and enzyme-linked immunosorbent assay (ELISA) have become household names for medical laboratories, manufacturers of in vitro diagnostic products, regulatory bodies, and external quality assessment and proficiency-testing organizations. (thefreedictionary.com)
  • The BED IgG-Capture Enzyme Immunoassay (BED assay) is a commercially available and widely used test for recent HIV infection. (nih.gov)
  • The assay is designed for use on all Roche immunoassay systems for low-, mid- and high-volume testing environments. (rttnews.com)
  • This new immunoassay was built for use with the Cobas e601 analyzer. (uspharmacist.com)
  • The purpose of this study is to demonstrate the ability of the Strep A Fluorescent Immunoassay Analyzer to accurately detect a throat swab specimen for the presence or absence of Strep A when compared to culture. (clinicaltrials.gov)
  • This benchtop immunoassay analyzer fits labs with space constraints and delivers fast, reliable results. (beckmancoulter.com)
  • This immunoassay analyzer gives technologists the benefit of ease of use without sacrificing throughput. (beckmancoulter.com)
  • ResearchMoz added Latest Research Report titled ' Global Chemiluminescence immunoassay analyzer Industry 2015: Market Forecasts for Liquefied Natural Gas (LNG), Oil Storage, Pipelines and Gas Processing ' to it's Large Report database. (sbwire.com)
  • Albany, NY -- ( SBWIRE ) -- 02/08/2016 -- The Global Chemiluminescence immunoassay analyzer Industry Report 2015 is a professional and in-depth study on the current state of the Chemiluminescence immunoassay analyzer industry. (sbwire.com)
  • The Chemiluminescence immunoassay analyzer market analysis is provided for the international markets including development trends, competitive landscape analysis, and key regions development status. (sbwire.com)
  • The Chemiluminescence immunoassay analyzer industry development trends and marketing channels are analyzed. (sbwire.com)
  • The Freedom EVOlyzer is a new enzyme immunoassay (EIA) analyzer that offers fully automated microplate processing. (thefreedictionary.com)
  • Some immunoassays can be carried out simply by mixing the reagents and sample and making a physical measurement. (wikipedia.org)
  • Radioactive isotopes can be incorporated into immunoassay reagents to produce a radioimmunoassay (RIA). (wikipedia.org)
  • SmartAssay test kits are complete immunoassays for analyzing pesticide residue, including all required reagents. (horiba.com)
  • Novel conjugates of protected aldehyde active metabolites of cyclophosphamide including reagents and immunogens thereof and monoclonal antibodies generated by these immunogens, said reagents and immunogens useful in immunoassays for the monitoring of the active metabolites of cyclophosphamide in patients being treated with cyclophosphamide. (freepatentsonline.com)
  • Among the various products and services available, the reagents and kits segment accounted for the largest share of the immunoassay market in 2014. (pitchengine.com)
  • This segment is also expected to grow at the highest CAGR during the forecast period, owing to the increase in immunoassay tests conducted due to rising aging population, rising number of chronic diseases, increased variety of reagents and the analytical techniques employed, trends like the high-growth at-home testing market, and accessibility to a wide range of reagents. (pitchengine.com)
  • In Vitro Diagnostic (IVD) Market [Instruments, Reagents & Data Management Systems] [Technique (Immunoassay, Clinical Chemistry, Molecular Diagnostics, Haematology) & Applications (Diabetes, Infectious Diseases, Cancer & Cardiology)] Systems, End Users] - Forecast To 2017. (pitchengine.com)
  • Global Immunoassay Reagents and Devices Market accounted to USD 16.25 billion in 2016 growing at a CAGR of 7.8% during the forecast period of 2017 to 2024. (openpr.com)
  • The global immunoassay reagents market is segmented on the basis of product type, technique, application, end-user, and geography. (openpr.com)
  • On the basis of product type the global immunoassay reagents market is segmented into reagents, analyzers, software and services. (openpr.com)
  • On the basis of application the global immunoassay reagents markt is segmented into infectious diseases, endocrinology, cardiology, oncology and others. (openpr.com)
  • The Global Immunoassay Reagents and Devices Market Report contains an in-depth profiling of the key market players, along with the recent developments (new product launches, partnerships, agreements, collaborations, and joint ventures) and strategies adopted by them to sustain and strengthen their positions in the market. (openpr.com)
  • The stringent regulatory procedures for the approval of the immunoassay products and the technological hurdles of the immunoassay kits and reagents have impacted the market. (marketsandmarkets.com)
  • Also, the technological hurdles posed by the immunoassay kits and reagents such as cross-reactivity of the antibodies, low detection limits of the available antibodies, false negatives and others decelerates the growth of the immunoassay market. (marketsandmarkets.com)
  • Reagents & kits segment dominated the immunoassay market in 2020. (marketsandmarkets.com)
  • Based on product, the immunoassay market is segmented into reagents & kits and analyzers. (marketsandmarkets.com)
  • Reagents & kits account for the largest share of the immunoassay market and is expected to grow at the highest CAGR. (marketsandmarkets.com)
  • In 2017, the reagents and kits segment accounted for the largest share of the immunoassays market. (webnewswire.com)
  • GenScript offers a broad range of protein detection and immunoassay products, including ONE-HOUR Western™ Detection System, Express™ ELISA Kits, Protein A ELISA Kit, cell lys ate products and related reagents for protein analysis that support your research. (genscript.com)
  • This guideline addresses components for harmonizing and assessing the quality of immunoassay systems for several commonly used dose-response indicator categories, e.g., radioisotopes, enzymes, fluorescence, luminescence, reagents, and experimental components criteria essential to characterizing an immunoassay. (clsi.org)
  • MARLBOROUGH, Mass. , April 28, 2016 /PRNewswire/ -- Web Industries, Inc., innovative provider of precision contract manufacturing and converting services, is proud to announce the opening of the company's first lateral flow immunoassay (LFI) production facility. (prnewswire.com)
  • I think 'Multiplex Flow Immunoassay' simply refers to the scientific method used in performing an ANA test. (healingwell.com)
  • Lateral Flow Immunoassay for Rapid Detection of Grapevine Leafroll-Associated Virus. (harvard.edu)
  • To further verify the result, we developed a multi-target lateral flow immunoassay strip (msLFIA) with staking pad design to obtain on-site rapid response for clinical diagnosis of PJI. (nature.com)
  • In addition, we developed a multi-target lateral flow immunoassay strip (msLFIA) with staking pad design to obtain on-site rapid response for clinical diagnosis of PJI, which the multi-target design is used to increase specificity and the stacking pad design is to enhance detection sensitivity. (nature.com)
  • Web Industries, Inc., a provider of precision contract manufacturing and converting services, opened the company's first lateral flow immunoassay production facility co-located within the company's existing location in Boston, Massachusetts . (areadevelopment.com)
  • Multi-step assays are often called separation immunoassays or heterogeneous immunoassays. (wikipedia.org)
  • Immunoassays that require separation of the bound Ab-Ag* complex are referred to as being heterogeneous immunoassays. (scribd.com)
  • Heterogeneous immunoassays require a physical separation between antibody‐bound and free analytes. (els.net)
  • Schematic representation of competitive limited‐reagent (a) and immunometric excess‐reagent (b) heterogeneous immunoassays. (els.net)
  • Photophysics of Heterogeneous Immunoassays (O.S. Khahil). (booktopia.com.au)
  • Qualitative immunoassays are often used to detect antigens on infectious agents and antibodies that the body produces to fight them. (encyclopedia.com)
  • For example, immunoassays are used to detect antigens on Hemophilus, Cryptococcus , and Streptococcus organisms in the cerebrospinal fluid (CSF) of meningitis patients. (encyclopedia.com)
  • Most, though not all, immunoassays involve chemically linking antibodies or antigens with some kind of detectable label. (wikipedia.org)
  • Immunoassays employ a variety of different labels to allow for detection of antibodies and antigens. (wikipedia.org)
  • An immunoassay is a test that uses immunocomplexing when antibodies and antigens are brought together. (scribd.com)
  • Multiplex immunoassays can be differentiated by the surface to which the antigens or antibodies are attached. (news-medical.net)
  • For the detection and quantitation of antibodies or antigens an immunoassay has to fulfill three basic requirements: highly specific, highly sensitive, and reproducible. (springer.com)
  • Antigen Quantification: Measurement of Multivalent Antigens by Solid-Phase Immunoassay (R.G. Hamilton). (booktopia.com.au)
  • RSV antigens were detected by rapid enzyme immunoassay in the nasal aspirates of 6. (thefreedictionary.com)
  • Immunoassays are chemical tests used to detect or quantify a specific substance, the analyte, in a blood or body fluid sample, using an immunological reaction. (encyclopedia.com)
  • In other words, in some immunoassays, the analyte may be an antibody rather than an antigen. (wikipedia.org)
  • Multiplex immunoassays and enzyme-linked immunosorbent assays (ELISA) are used to determine and analyze the presence of an analyte in a sample through the binding of complimentary antigen-antibody pairs. (news-medical.net)
  • The present invention is directed to a membrane-based immunoassay method for an analyte of interest having at least two sterically separate antigenic sites. (google.com.au)
  • Our microfluidic method facilitates hands-free saliva analysis by integrating sample pretreatment (filtering, enrichment, mixing) with electrophoretic immunoassays to quickly measure analyte concentrations in minimally pretreated saliva samples. (pnas.org)
  • The invention concerns a method for the detection of an analyte in a sample liquid by an enzyme-immunoassay in which an enzyme-labelled compound is partitioned between a solid and a liquid phase and the amount of enzyme label in the liquid phase outside the solid phase is determined as a measure of the. (google.co.uk)
  • While ELISA is still commonly used to characterise a single analyte, laboratories and organisations are moving towards multiplex immunoassays. (intechopen.com)
  • Based on the sandwich immunoassay principle, a test strip was made of a nitrocellulose membrane dotted with monoclonal antibodies against VCO1 as analyte capture molecules. (hindawi.com)
  • Key players in the immunoassay market include are Roche Diagnostics (Switzerland), Siemens Healthcare (Germany), Abbott Laboratories (U.S.), Beckman Coulter (U.S.), Ortho Clinical Diagnostics (U.S.), and bioMérieux (France). (pitchengine.com)
  • On the basis of technology, the immunoassay market is segmented into ELISA, rapid tests, western blotting, enzyme-linked immunospot (ELISPOT), immuno-PCR, and other immunoassay technologies. (webnewswire.com)
  • Immunoassays may be qualitative (positive or negative) or quantitative (amount measured). (encyclopedia.com)
  • Quantitative immunoassays are performed by measuring the signal produced by the indicator reaction. (encyclopedia.com)
  • A newer approach to immunoassays involves combining real-time quantitative polymerase chain reaction (RT qPCR) and traditional immunoassay techniques. (wikipedia.org)
  • cGMP Enzyme Immunoassay Kit has been used for the quantitative determination of cyclic guanine monophosphate (cGMP) concentration in parasite tissues and mouse aortic artery homogenates. (sigmaaldrich.com)
  • Our multiplex immunoassays use the FirePlex ® particle technology to enable the quantitative measurement of many human proteins and peptides at the same time using as little as 12.5 μL of sample per well. (abcam.com)
  • The microengraving immunoassay is based on intaglio printing, in which a glass slide is temporarily sealed to the array of nanowells to capture proteins secreted by confined cells in both a multiplexed and quantitative manner. (mdpi.com)
  • OTCQX: RHHBY) today announced the launch of the Elecsys Anti-p53 immunoassay for the in vitro quantitative determination of anti-p53 antibodies. (roche.com)
  • Anti-p53 Elecsys Anti-p53 immunoassay for the in vitro quantitative determination of anti-p53 autoantibodies in human serum and plasma. (roche.com)
  • Intended as an aid in the in the diagnosis of myocardial infarction, the electrochemiluminescence (ECL) immunoassay is used for the in vitro quantitative determination of TnT-hs in human serum and plasma, the company said. (rttnews.com)
  • The IMMULITE chemiluminescent immunoassay system is designed for ease of use, reliability, high-performance assays and fully integrated operation. (bio-medicine.org)
  • Developed by Roche Diagnostics, the Elecsys anti-HCV immunoassay is used to detect anti-HCV through the use of electrochemiluminescence (ECL) technology. (uspharmacist.com)
  • With the completion of this acquisition, Abbott strengthened its diagnostics portfolio and established itself as a leading player in the point-of-care testing and immunoassays market. (marketsandmarkets.com)
  • The increasing significance of the companion diagnostics, technological progress of immunoassay products, condition-specific biomarkers immunoassays and other emerging technologies are propelling the growth of the immunoassay market. (marketsandmarkets.com)
  • The use of the automated immunoassay analyzers for cardiac ailments, endocrine diseases, and cancer has observed a rise since the last few years, owing to the accurate and speedy diagnosis capability of the product. (openpr.com)
  • The immunoassay market in several countries have witnessed massive growth amid the pandemic, owing to the mass scale diagnosis of COVID-19. (marketsandmarkets.com)
  • Immunoassay diagnosis has become an instrumental part of the diagnostic regime of these diseases. (marketsandmarkets.com)
  • The massive adoption of the rapid tests and point-of-care testing for diseases diagnosis and drug and alcohol abuse have thrusted the growth spurt of the global immunoassay market. (marketsandmarkets.com)
  • Enzyme immunoassay in the diagnosis of Lyme borreliosis. (prohealth.com)
  • This innovative multiplex immunoassay is a powerful alternative to ELISAs and VNTs for veterinary diagnosis of flavivirus-related diseases. (hindawi.com)
  • LAB Maastricht UMC+ in the Netherlands has a long history with myasthenia gravis, using gold standard immunoassays to enable accurate diagnosis and provide the information necessary to allow effective treatment. (tecan.com)
  • Immuno modules -These modules are designed for solid phase immunoassays. (thermofisher.com)
  • Immunochemical Considerations in the Analysis of Data from Non-Competitive Solid-Phase Immunoassays (J.H. Peterman). (booktopia.com.au)
  • Considerations of the Interpretation of the Specificity of Monoclonal Antibodies Determined in Solid-Phase Immunoassays (F.J. Stevens). (booktopia.com.au)
  • Mass spectrometric immunoassay ( MSIA ) is a method that uses antibodies to analyze protein differences in biological samples. (wikipedia.org)
  • The new Thermo Scientific Mass Spectrometric Immunoassay (MSIA) provides a robust technology designed to purify target proteins, peptides and therapeutic antibodies in a simplified way for downstream mass spectrometric analysis. (businesswire.com)
  • Immunoassays which employ enzymes are referred to as enzyme immunoassays (EIAs), of which enzyme-linked immunosorbent assays (ELISAs) and enzyme multiplied immunoassay technique (EMIT) are the most common types. (wikipedia.org)
  • The first article in this two-article series ( Automating and Miniaturizing Immunoassays: Starting the Switch to Maximum Productivity ) describes the Gyrolab™ immunoassay technology platform, focusing on its ability to save time during biopharma development and to generate higher-quality data compared with traditional enzyme-linked immunosorbent assays (ELISAs). (genengnews.com)
  • The simplest immunoassay method measures the quantity of precipitate, which forms after the reagent antibody (precipitin) has incubated with the sample and reacted with its respective antigen to form an insoluble aggregate. (encyclopedia.com)
  • Miniaturizing immunoassays can deliver tremendous savings in sample volumes, animal use, and reagent consumption. (genengnews.com)
  • Immunoassay analyzers are used to identify and detect the concentration of specific substances in a sample, usually using an antibody as a reagent. (beckmancoulter.com)
  • Immunoassay is the method of choice for measuring analytes normally present at very low concentrations that cannot be determined accurately by other less expensive tests. (encyclopedia.com)
  • Analytes in biological liquids such as serum or urine are frequently measured using immunoassays for medical and research purposes. (wikipedia.org)
  • Immunoassays use one or more select antibodies to detect analytes of interest. (scribd.com)
  • The validation of novel biomarkers and their amalgamation into multiplex immunoassays confers the prospects of simultaneous measurement of multiple analytes in a single sample, thereby minimising cost, time and sample. (intechopen.com)
  • The mass spec immunoassay (MSIA) workflow was developed to provide a simple, automated process for purification of targeted analytes for downstream detection using multiple analytical techniques, such as mass spectrometry. (tecan.com)
  • Immunoassays are analytical techniques that are widely used to detect and quantitate the concentration of a protein, metabolite, or drug in a biological sample. (els.net)
  • Immunoassay analyses reduce costs by providing real-time data, thus reducing the time spent waiting for analytical results and the amount of material that has to be excavated. (cpeo.org)
  • The analytical applicability of the presented immunoassay is verified by CRP measurements in human blood serum samples. (mdpi.com)
  • Analytical Comparison of the Architect Syphilis TP and Liaison Treponema Automated Chemiluminescent Immunoassays and Their Performance in a Reverse Syphilis Screening Algorithm. (harvard.edu)
  • Although immunoanalytical techniques are established and advantageous over alternative screening analytical platforms, one of the challenges for immunoassays is multiplexing. (intechopen.com)
  • The emergence of automation in the medical sector is one of the many drivers that will fuel automated immunoassay analyzers market. (openpr.com)
  • The deployment of automation in the current and forthcoming product portfolio will lead to consumers giving preference to automated immunoassay analyzers, which is likely to boost automated immunoassay analyzers industry size noticeably. (openpr.com)
  • According to Global Market Insights, Inc., "Worldwide automated immunoassay analyzers market having had a revenue of USD 8 billion in 2016, will collect a revenue of USD 25 billion by 2024 with an annual growth rate of more than 15% over 2017-2024. (openpr.com)
  • Speaking of which, automated immunoassay analyzers industry share from hospitals is slated to generate considerable revenue by 2024. (openpr.com)
  • India is one of those regions that is likely to observe exponential growth in automated immunoassay analyzers market. (openpr.com)
  • In addition, the government has also been promoting public & private partnerships to provide excellent medical service mainly across rural areas, which consequentially, will fuel Asia Pacific automated immunoassay analyzers market share considerably. (openpr.com)
  • These analyzers have been gaining prominence among consumers subject to the requirement of monitoring therapeutic drugs, which is likely to augment automated immunoassay analyzers industry size substantially. (openpr.com)
  • On these grounds, automated immunoassay analyzers market size from infectious diseases will collect a revenue of more than USD 7 billion by 2024. (openpr.com)
  • To carry out vital activities like analysis and evaluation of test results, this product is prominently used in various diagnostic laboratories, subject to which automated immunoassay analyzers market share from diagnostic laboratories will exhibit the highest annual growth rate over the coming timeframe. (openpr.com)
  • The emergence of software based technological advancements in instruments to perform multiple tests at the same time is also bound to favorably enhance the automated immunoassay analyzers industry growth. (openpr.com)
  • The United States has been one of lucrative business avenues for automated immunoassay analyzers market. (openpr.com)
  • Pertaining to the same, U.S. dominated North America automated immunoassay analyzers market share in 2016, by accounting for 90% of the regional contribution. (openpr.com)
  • The U.S. government is also promoting the usage of automated products by implementing supportive regulatory norms, which will significantly propel U.S. automated immunoassay analyzers market share. (openpr.com)
  • Chemiluminescence, basically a chemical reaction, constitutes one of the lucrative product segments of automated immunoassay analyzers industry. (openpr.com)
  • The massive research, valuations and approvals of several immunoassay consumables and analyzers have alleviated the issues of the SARS-CoV2 detection. (marketsandmarkets.com)
  • Beckman Coulter offers a variety of scalable immunoassay analyzers and assays, all of which can help optimize your laboratory's uptime, reliability and performance. (beckmancoulter.com)
  • Extend the services of your core laboratory with our range of immunoassay analyzers and comprehensive portfolio of assays. (beckmancoulter.com)
  • The new Elecsys Anti-p53 immunoassay uses the well-established electrochemiluminescence immunoassay "ECLIA" technology and is intended for use on cobas e immunoassay analyzers. (roche.com)
  • This high growth can be attributed to time-effectiveness and cost effectiveness provided by mass spectrometry as compared to the enzyme assays and immunoassays technology. (prnewswire.com)
  • Because of the complexity and long time commitments involved in the development of such assays, particularly highly specific or efficient assays and immunoassays, many pharmaceutical companies, biotechs, and academic institutions have found that it becomes a bottleneck, holding up valuable research. (genscript.com)
  • Discover the benefits of automation in the mass spec immunoassay (MSIA) workflow. (tecan.com)
  • Schematic representation of a homogeneous immunoassay in which, when present, the sample antigen binds to the antibody, thereby allowing access of a substrate to the active site of an enzyme labelled with the antigen. (els.net)
  • Multiplex immunoassay kits, even from the same manufacturer, may not give identical results. (news-medical.net)
  • Retrieved on January 28, 2020 from https://www.news-medical.net/life-sciences/Designing-a-Multiplex-Immunoassay-Factors-to-Consider.aspx. (news-medical.net)
  • b) Multiplex Immunoassay Protein Standard Mix, such as ab221503 . (abcam.com)
  • In a competitive immunoassay. (scribd.com)
  • cGMP Enzyme Immunoassay Kit is non-radioactive, competitive immunoassay for the quantitation of cyclic guanine monophosphate (cGMP) in biological fluids (serum, plasma, urine and saliva), tissue culture media samples or in samples containing very low concentrations of cyclic nucleotides. (sigmaaldrich.com)
  • Non-radioactive, competitive immunoassay for the quantitation of cGMP in biological fluids (serum, plasma, urine and saliva), tissue culture media samples, or in samples containing very low concentrations of cyclic nucleotides. (sigmaaldrich.com)
  • The recent study by Fact.MR on global Chemiluminescence Immunoassays (CLIA) market offers a 5-year forecast between 2020 and 2025. (bccresearch.com)
  • The study analyzes crucial trends that are currently determining the growth of the global chemiluminescence immunoassays (CLIA) market. (bccresearch.com)
  • The study also provides dynamics that are responsible for influencing the future status of the global chemiluminescence immunoassays (CLIA) market over the forecast period. (bccresearch.com)
  • A detailed assessment of the global chemiluminescence immunoassays (CLIA) market value chain analysis, business execution, and supply chain analysis across the regional markets has been covered in the report. (bccresearch.com)
  • A list of prominent companies operating in the global chemiluminescence immunoassays (CLIA) market along with their product portfolio enhances the reliability of this comprehensive research study. (bccresearch.com)
  • The study offers a comprehensive analysis on diverse features including production capacities, demand, product developments, revenue generation and sales in the global chemiluminescence immunoassays (CLIA) market across the globe. (bccresearch.com)
  • A comprehensive estimate on the global chemiluminescence immunoassays (CLIA) market has been provided through an optimistic as well as a conservative scenario, taking into account the sales of global chemiluminescence immunoassays (CLIA) devices during the forecast period. (bccresearch.com)
  • The global chemiluminescence immunoassays (CLIA) market has been analyzed for each market segment, in terms of value (US$ Mn). (bccresearch.com)
  • Market estimates at global and regional levels for global chemiluminescence immunoassays (CLIA) is available in terms of "US$ Mn" for value, along with market attractiveness evaluation in the report. (bccresearch.com)
  • Key sections have been elaborated in the global chemiluminescence immunoassays (CLIA) market report, which have helped to deliver projection on the regional markets. (bccresearch.com)
  • These chapters include the regional macros (economic and business environment outlook), which are expected to have a momentous influence on the growth of global chemiluminescence immunoassays (CLIA) market during the forecast period. (bccresearch.com)
  • Country-specific valuation on demand for global chemiluminescence immunoassays (CLIA) has been offered for each regional market, along with the market scope estimate and forecast, price index, and impact analysis of dynamics of prominence in regions and countries. (bccresearch.com)
  • The report sheds light on the leading manufacturers of global chemiluminescence immunoassays (CLIA) devices along with their detailed profiles. (bccresearch.com)
  • Essential and up-to-date data information related to the market performers, who are principally engaged in the production of global chemiluminescence immunoassays (CLIA) devices, has been brought with the help of a detailed dashboard view. (bccresearch.com)
  • Company presence is mapped and presented through the matrix for all the prominent players thus providing the readers with actionable insights, which helps in thoughtfully presenting market status, and predicting the competition level in global chemiluminescence immunoassays (CLIA) market. (bccresearch.com)
  • This report analyzes the worldwide markets for Immunoassay Systems in US$ Million by the following product segments - Enzyme Immunoassay Systems, Fluorescence Immunoassay Systems, Chemiluminescence Immunoassay Systems, Radio Immunoassay Systems, and Nephelometric Immunoassay Systems. (mdtmag.com)
  • Drug‐of‐abuse immunoassay testing on a chemistry analyser versus a microchip array. (els.net)
  • Thyrotropin (TSH), free thyroxine (fT4) and testosterone assays have been used as a probe to evaluate the performances of a new modular chemiluminescence (CL) immunoassay analyser, the Abbott Architect™ i 2000. (degruyter.com)
  • BioCheck is a provider of high quality enzyme immunoassay research services and products, and a leading provider of immunoassay kits for cardiac and tumor biomarkers, infectious diseases, thyroid function, steroids, and fertility hormones. (thefreedictionary.com)
  • There are a variety of detectors used in immunoassays such as enzymes, fluorophores, luminescent tags, electrochemical reactions and immunosensors. (els.net)
  • Hence, we have developed a simple, cost effective, and label-free electrochemical immunoassay for the measurement of CRP in a drop of serum sample using an immunosensor strip made up of a screen printed carbon electrode (SPE) modified with anti-CRP functionalized gold nanoparticles (AuNPs). (mdpi.com)
  • A new homogeneous electrochemical immunoassay strategy was developed for ultrasensitive detection of carcinoembryonic antigen (CEA) based on target-induced proximity hybridization coupled with rolling circle amplification (RCA). (rsc.org)
  • Immunoassay control set for Human Interleukin 18 protein levels. (fishersci.com)
  • The instant application discloses a protein calibrator for use in the NT-proBNP immunoassays disclosed in application Ser. (freepatentsonline.com)
  • 0002] This invention relates to the expression and purification of proteins, more particularly to the expression and purification of a protein calibrator used for standardization of an immunoassay, and most particularly to the expression and purification of NT-BNP used to calibrate standards in an immunoassay which measures the concentration of NT-proBNP in bodily fluids. (freepatentsonline.com)
  • Sensitive immunoassay that uses an enzyme linked to an antibody or antigen as a marker for the detection of a specific protein, especially an antigen or antibody. (citizendium.org)
  • By taking advantage of the Luminex ® technology, we have developed two multiplex immunoassays to monitor protein targets of depletion or retention. (sigmaaldrich.com)
  • A novel immunoassay that identifies and quantifies blood levels of the PAM4 protein may detect early-stage pancreatic cancer, according to study findings presented ahead of the 2010 Gastrointestinal Cancers Symposium. (healio.com)
  • Microengraving is a novel immunoassay forcharacterizing multiple protein secretions from single cells. (mdpi.com)
  • Our study directly validates that protein secretion rates can be quantified by the microengraving immunoassay. (mdpi.com)
  • Immunoassay using antibody-enzyme conjugates. (springer.com)
  • Enzyme (EIA) immunoassay was developed as an alternative to radioimmunoassay (RIA). (encyclopedia.com)
  • Radioimmunoassay, enzyme and non-enzyme-based immunoassays. (springer.com)
  • Immunoassays measure the formation of antibody-antigen complexes and detect them via an indicator reaction. (encyclopedia.com)
  • In some cases, an immunoassay may use an antigen to detect for the presence of antibodies, which recognize that antigen, in a solution. (wikipedia.org)
  • Immunoassay tests are designed to detect specific chemicals by measuring the chemicals' response to specific antibodies. (cpeo.org)
  • Using the conventional monoplex immunoassay ELISA technique as a benchmark, the procedure was used to detect several grapevine and tree fruit viruses. (nih.gov)
  • Dona, V., 1985, Homogeneous colorimetric enzyme inhibition immunoassay for cortisol in human serum with Fab anti-glucose-6-phosphate dehydrogenase as a label modulator, J. Immunol. (springer.com)
  • On binding, they bridge a solid support, facilitating their incorporation into a format of immunoassay for particular quantification. (news-medical.net)
  • The researchers used the PAM4-serum enzyme immunoassay for the detection and quantification of PAM4 in the serum of 68 patients who had surgical resection of pancreatic cancer and compared them with 19 healthy participants. (healio.com)
  • Linearity of dilution (natural linearity, spiked linearity) is another important fundamental of immunoassay accuracy and specificity. (rndsystems.com)
  • 51 monoclonal antibodies (McAb) with putative specificity for human IgA, the IgA subclasses, Am allotypes or secretory component (SC) were evaluated for immunoreactivity and specificity by nine laboratories employing immunodiffusion, agglutination, immunohistological assays, immunoblotting and direct binding and competitive inhibition enzyme immunoassays. (tudelft.nl)
  • Immunoassay determinations of the serum levels of certain proteins or carbohydrates serve as tumor markers. (cms.gov)
  • We offer two basic categories of services: 1) Immunoassay development service for drug compound discovery and 2) immunoassay development service for therapeutic antibody detection. (genscript.com)
  • This type of immunoassay is now used in around 100 million clinical tests every year worldwide, enabling clinicians to measure a wide range of proteins, pathogens and other molecules in blood samples. (wikipedia.org)
  • Quantify up to 75 proteins and peptides per well, using just 12.5 µL of sample, with FirePlex ® immunoassays. (abcam.com)
  • This invention relates to an element for immunoassay. (google.ca)
  • The present invention relates to immunoassays, and, more particularly, to improved microscale diffusion immunoassays utilizing multivalent reactants. (google.co.uk)
  • Taking an interdisciplinary approach that emphasizes the adaptability of immunochemical and related bioanalytical methods to a variety of matrices, Immunoassay and Other Bioanalytical Techniques describes the strength and the versatility of these methods in a wide range of environmental and biological measurement applications. (routledge.com)
  • Immunoassay methods are common and commercially available. (cpeo.org)
  • The PMR report offers an assessment of the key dynamics of the immunoassay interference blocker market which includes market drivers, industry trends, and opportunities held by the market. (bccresearch.com)
  • The immunoassay interference blocker market report also studies the significant market dynamics including market drivers, trends, and restraints. (bccresearch.com)
  • This report explicates on vital dynamics such as the drivers, restraints and opportunities for key market players along with key stakeholders as well as emerging players associated with the development of chemiluminescence immunoassays (CLIA). (bccresearch.com)
  • Persistence Market Research (PMR) recently published a report on the immunoassay interference blocker market that provides readers with an overall market outlook with the help of an extensive market analysis. (bccresearch.com)
  • The PMR report on immunoassay interference blocker market examines the historical and current market scenario and offers a forecast for the period, 2019-2029. (bccresearch.com)
  • The PMR study helps companies in this landscape gain a complete idea of the market and aid in taking key business decisions with the help of vital findings and exclusive insights into the immunoassay interference blocker market. (bccresearch.com)
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  • How much will the immunoassay interference blocker market value in the 2029? (bccresearch.com)
  • What are key trends that are likely to shape the immunoassay interference blocker market in the coming years? (bccresearch.com)
  • Which type is likely to remain the sought-after immunoassay interference blocker type over the next ten years? (bccresearch.com)
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  • PMR's study on immunoassay interference blocker market begins with an executive summary that sheds light on the various immunoassay interference blocker market aspects covered in the report. (bccresearch.com)
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  • This product is the result of years of R&D aimed at creating the most powerful immunoassay interference blocker in the market. (biospace.com)
  • Immunoassays used to assess thyroid function are vulnerable to different types of interference that may affect clinical decision-making. (degruyter.com)
  • For this reason, RIA has been largely replaced in routine clinical laboratory practice by enzyme immunoassay. (encyclopedia.com)
  • Whether you run dozens or thousands of tests per day, you can rely on the same innovation and quality in our immunoassay instruments and systems that you know and trust from the rest of our total laboratory solution portfolio. (beckmancoulter.com)
  • This immunoassay system supports the expansion needs of your growing laboratory. (beckmancoulter.com)