Immunoassay
Immunoenzyme Techniques
Fluorescence Polarization Immunoassay
Enzyme Multiplied Immunoassay Technique
Reagent Kits, Diagnostic
Sensitivity and Specificity
Enzyme-Linked Immunosorbent Assay
An immunoassay utilizing an antibody labeled with an enzyme marker such as horseradish peroxidase. While either the enzyme or the antibody is bound to an immunosorbent substrate, they both retain their biologic activity; the change in enzyme activity as a result of the enzyme-antibody-antigen reaction is proportional to the concentration of the antigen and can be measured spectrophotometrically or with the naked eye. Many variations of the method have been developed.
Fluoroimmunoassay
Radioimmunoassay
Classic quantitative assay for detection of antigen-antibody reactions using a radioactively labeled substance (radioligand) either directly or indirectly to measure the binding of the unlabeled substance to a specific antibody or other receptor system. Non-immunogenic substances (e.g., haptens) can be measured if coupled to larger carrier proteins (e.g., bovine gamma-globulin or human serum albumin) capable of inducing antibody formation.
Cross Reactions
False Positive Reactions
Evaluation Studies as Topic
Luminescent Measurements
Substance Abuse Detection
Immunoglobulin G
Nephelometry and Turbidimetry
Chemical analysis based on the phenomenon whereby light, passing through a medium with dispersed particles of a different refractive index from that of the medium, is attenuated in intensity by scattering. In turbidimetry, the intensity of light transmitted through the medium, the unscattered light, is measured. In nephelometry, the intensity of the scattered light is measured, usually, but not necessarily, at right angles to the incident light beam.
Europium
Reproducibility of Results
The statistical reproducibility of measurements (often in a clinical context), including the testing of instrumentation or techniques to obtain reproducible results. The concept includes reproducibility of physiological measurements, which may be used to develop rules to assess probability or prognosis, or response to a stimulus; reproducibility of occurrence of a condition; and reproducibility of experimental results.
Antibody Specificity
Gas Chromatography-Mass Spectrometry
Immunoglobulin M
False Negative Reactions
Antibodies, Immobilized
Latex Fixation Tests
Digoxin
A cardiotonic glycoside obtained mainly from Digitalis lanata; it consists of three sugars and the aglycone DIGOXIGENIN. Digoxin has positive inotropic and negative chronotropic activity. It is used to control ventricular rate in ATRIAL FIBRILLATION and in the management of congestive heart failure with atrial fibrillation. Its use in congestive heart failure and sinus rhythm is less certain. The margin between toxic and therapeutic doses is small. (From Martindale, The Extra Pharmacopoeia, 30th ed, p666)
Quality Control
Reference Standards
Feces
Calibration
Indicators and Reagents
Substances used for the detection, identification, analysis, etc. of chemical, biological, or pathologic processes or conditions. Indicators are substances that change in physical appearance, e.g., color, at or approaching the endpoint of a chemical titration, e.g., on the passage between acidity and alkalinity. Reagents are substances used for the detection or determination of another substance by chemical or microscopical means, especially analysis. Types of reagents are precipitants, solvents, oxidizers, reducers, fluxes, and colorimetric reagents. (From Grant & Hackh's Chemical Dictionary, 5th ed, p301, p499)
Fluorescent Antibody Technique
Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
Antibodies
Clinical Laboratory Techniques
Microspheres
Reference Values
Biological Markers
Measurable and quantifiable biological parameters (e.g., specific enzyme concentration, specific hormone concentration, specific gene phenotype distribution in a population, presence of biological substances) which serve as indices for health- and physiology-related assessments, such as disease risk, psychiatric disorders, environmental exposure and its effects, disease diagnosis, metabolic processes, substance abuse, pregnancy, cell line development, epidemiologic studies, etc.
Microchemistry
Chromatography, High Pressure Liquid
Phenytoin
An anticonvulsant that is used to treat a wide variety of seizures. It is also an anti-arrhythmic and a muscle relaxant. The mechanism of therapeutic action is not clear, although several cellular actions have been described including effects on ion channels, active transport, and general membrane stabilization. The mechanism of its muscle relaxant effect appears to involve a reduction in the sensitivity of muscle spindles to stretch. Phenytoin has been proposed for several other therapeutic uses, but its use has been limited by its many adverse effects and interactions with other drugs.
Limit of Detection
Antibodies, Heterophile
Antibodies elicited in a different species from which the antigen originated. These antibodies are directed against a wide variety of interspecies-specific antigens, the best known of which are Forssman, Hanganutziu-Deicher (H-D), and Paul-Bunnell (P-B). Incidence of antibodies to these antigens--i.e., the phenomenon of heterophile antibody response--is useful in the serodiagnosis, pathogenesis, and prognosis of infection and latent infectious states as well as in cancer classification.
Fluorescence Polarization
Measurement of the polarization of fluorescent light from solutions or microscopic specimens. It is used to provide information concerning molecular size, shape, and conformation, molecular anisotropy, electronic energy transfer, molecular interaction, including dye and coenzyme binding, and the antigen-antibody reaction.
alpha-Fetoproteins
Immunoradiometric Assay
Radioimmunoprecipitation Assay
Sensitive assay using radiolabeled ANTIGENS to detect specific ANTIBODIES in SERUM. The antigens are allowed to react with the serum and then precipitated using a special reagent such as PROTEIN A sepharose beads. The bound radiolabeled immunoprecipitate is then commonly analyzed by gel electrophoresis.
Predictive Value of Tests
In screening and diagnostic tests, the probability that a person with a positive test is a true positive (i.e., has the disease), is referred to as the predictive value of a positive test; whereas, the predictive value of a negative test is the probability that the person with a negative test does not have the disease. Predictive value is related to the sensitivity and specificity of the test.
Immune Sera
Rubella virus
Latex
A milky, product excreted from the latex canals of a variety of plant species that contain cauotchouc. Latex is composed of 25-35% caoutchouc, 60-75% water, 2% protein, 2% resin, 1.5% sugar & 1% ash. RUBBER is made by the removal of water from latex.(From Concise Encyclopedia Biochemistry and Molecular Biology, 3rd ed). Hevein proteins are responsible for LATEX HYPERSENSITIVITY. Latexes are used as inert vehicles to carry antibodies or antigens in LATEX FIXATION TESTS.
Immunologic Techniques
Streptavidin
Rotavirus
Thyroxine
The major hormone derived from the thyroid gland. Thyroxine is synthesized via the iodination of tyrosines (MONOIODOTYROSINE) and the coupling of iodotyrosines (DIIODOTYROSINE) in the THYROGLOBULIN. Thyroxine is released from thyroglobulin by proteolysis and secreted into the blood. Thyroxine is peripherally deiodinated to form TRIIODOTHYRONINE which exerts a broad spectrum of stimulatory effects on cell metabolism.
Seroepidemiologic Studies
Saliva
Polymerase Chain Reaction
In vitro method for producing large amounts of specific DNA or RNA fragments of defined length and sequence from small amounts of short oligonucleotide flanking sequences (primers). The essential steps include thermal denaturation of the double-stranded target molecules, annealing of the primers to their complementary sequences, and extension of the annealed primers by enzymatic synthesis with DNA polymerase. The reaction is efficient, specific, and extremely sensitive. Uses for the reaction include disease diagnosis, detection of difficult-to-isolate pathogens, mutation analysis, genetic testing, DNA sequencing, and analyzing evolutionary relationships.
Horseradish Peroxidase
Specimen Handling
Pregnancy
Fluorescent Antibody Technique, Direct
Serum
Biological Assay
A method of measuring the effects of a biologically active substance using an intermediate in vivo or in vitro tissue or cell model under controlled conditions. It includes virulence studies in animal fetuses in utero, mouse convulsion bioassay of insulin, quantitation of tumor-initiator systems in mouse skin, calculation of potentiating effects of a hormonal factor in an isolated strip of contracting stomach muscle, etc.
Gastroenteritis
Rabbits
Chorionic Gonadotropin, beta Subunit, Human
The beta subunit of human CHORIONIC GONADOTROPIN. Its structure is similar to the beta subunit of LUTEINIZING HORMONE, except for the additional 30 amino acids at the carboxy end with the associated carbohydrate residues. HCG-beta is used as a diagnostic marker for early detection of pregnancy, spontaneous abortion (ABORTION, SPONTANEOUS); ECTOPIC PREGNANCY; HYDATIDIFORM MOLE; CHORIOCARCINOMA; or DOWN SYNDROME.
Avidin
Blood Specimen Collection
Colorimetry
Clostridium difficile
Luminescence
Antibody Affinity
A measure of the binding strength between antibody and a simple hapten or antigen determinant. It depends on the closeness of stereochemical fit between antibody combining sites and antigen determinants, on the size of the area of contact between them, and on the distribution of charged and hydrophobic groups. It includes the concept of "avidity," which refers to the strength of the antigen-antibody bond after formation of reversible complexes.
Biotin
Lanthanoid Series Elements
Antigen-Antibody Complex
Rubella
Microfluidic Analytical Techniques
Virology
Rotavirus Infections
Autoantibodies
Thyrotropin
A glycoprotein hormone secreted by the adenohypophysis (PITUITARY GLAND, ANTERIOR). Thyrotropin stimulates THYROID GLAND by increasing the iodide transport, synthesis and release of thyroid hormones (THYROXINE and TRIIODOTHYRONINE). Thyrotropin consists of two noncovalently linked subunits, alpha and beta. Within a species, the alpha subunit is common in the pituitary glycoprotein hormones (TSH; LUTEINIZING HORMONE and FSH), but the beta subunit is unique and confers its biological specificity.
Biosensing Techniques
Chorionic Gonadotropin
A gonadotropic glycoprotein hormone produced primarily by the PLACENTA. Similar to the pituitary LUTEINIZING HORMONE in structure and function, chorionic gonadotropin is involved in maintaining the CORPUS LUTEUM during pregnancy. CG consists of two noncovalently linked subunits, alpha and beta. Within a species, the alpha subunit is virtually identical to the alpha subunits of the three pituitary glycoprotein hormones (TSH, LH, and FSH), but the beta subunit is unique and confers its biological specificity (CHORIONIC GONADOTROPIN, BETA SUBUNIT, HUMAN).
Fluorometry
Hepatitis A Antigens
Point-of-Care Systems
Binding, Competitive
Agglutination Tests
Opiate Alkaloids
Immunoglobulin A
Rheumatoid Factor
Barbiturates
Immunoblotting
Cardenolides
C(23)-steroids with methyl groups at C-10 and C-13 and a five-membered lactone at C-17. They are aglycone constituents of CARDIAC GLYCOSIDES and must have at least one double bond in the molecule. The class includes cardadienolides and cardatrienolides. Members include DIGITOXIN and DIGOXIN and their derivatives and the STROPHANTHINS.
Blotting, Western
Hepatitis Antibodies
Theophylline
A methyl xanthine derivative from tea with diuretic, smooth muscle relaxant, bronchial dilation, cardiac and central nervous system stimulant activities. Theophylline inhibits the 3',5'-CYCLIC NUCLEOTIDE PHOSPHODIESTERASE that degrades CYCLIC AMP thus potentiates the actions of agents that act through ADENYLYL CYCLASES and cyclic AMP.
Reagent Strips
Digoxigenin
Narcotics
Street Drugs
Nordazepam
Complement Fixation Tests
Serologic tests based on inactivation of complement by the antigen-antibody complex (stage 1). Binding of free complement can be visualized by addition of a second antigen-antibody system such as red cells and appropriate red cell antibody (hemolysin) requiring complement for its completion (stage 2). Failure of the red cells to lyse indicates that a specific antigen-antibody reaction has taken place in stage 1. If red cells lyse, free complement is present indicating no antigen-antibody reaction occurred in stage 1.
Molecular Sequence Data
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
Tandem Mass Spectrometry
A mass spectrometry technique using two (MS/MS) or more mass analyzers. With two in tandem, the precursor ions are mass-selected by a first mass analyzer, and focused into a collision region where they are then fragmented into product ions which are then characterized by a second mass analyzer. A variety of techniques are used to separate the compounds, ionize them, and introduce them to the first mass analyzer. For example, for in GC-MS/MS, GAS CHROMATOGRAPHY-MASS SPECTROMETRY is involved in separating relatively small compounds by GAS CHROMATOGRAPHY prior to injecting them into an ionization chamber for the mass selection.
Amphetamines
Hemagglutination Tests
Cattle
Polystyrenes
Enterocolitis, Pseudomembranous
An acute inflammation of the INTESTINAL MUCOSA that is characterized by the presence of pseudomembranes or plaques in the SMALL INTESTINE (pseudomembranous enteritis) and the LARGE INTESTINE (pseudomembranous colitis). It is commonly associated with antibiotic therapy and CLOSTRIDIUM DIFFICILE colonization.
Oxymorphone
An opioid analgesic with actions and uses similar to those of MORPHINE, apart from an absence of cough suppressant activity. It is used in the treatment of moderate to severe pain, including pain in obstetrics. It may also be used as an adjunct to anesthesia. (From Martindale, The Extra Pharmacopoeia, 30th ed, p1092)
Carcinoembryonic Antigen
Antigen-Antibody Reactions
Protein Array Analysis
Galactosidases
Hepatitis B Surface Antigens
Pregnanediol
Automation
Alkaline Phosphatase
Microfluidics
Gentamicins
Case-Control Studies
Studies which start with the identification of persons with a disease of interest and a control (comparison, referent) group without the disease. The relationship of an attribute to the disease is examined by comparing diseased and non-diseased persons with regard to the frequency or levels of the attribute in each group.
Thyroxine-Binding Proteins
Dimethylpolysiloxanes
Silicone polymers which consist of silicon atoms substituted with methyl groups and linked by oxygen atoms. They comprise a series of biocompatible materials used as liquids, gels or solids; as film for artificial membranes, gels for implants, and liquids for drug vehicles; and as antifoaming agents.
Acecainide
Urinalysis
Peptide Fragments
Mamastrovirus
Nasopharynx
Drug Monitoring
Giardiasis
Chemistry, Clinical
Mass Spectrometry
Immunodiffusion
HIV Antigens
beta-Galactosidase
Immunological comparison of the proteins of chicken and rat liver ribosomes. (1/4669)
A comparison of the proteins of chicken and rat liver ribosomes using immunochemical techniques was undertaken. The procedures included quantitative precipitation, passive hemagglutination, and immunodiffusion on Ouchterlony plates. The results indicate that antisera specific for chicken or rat liver ribosomes recognize only about 20% of common determinants. While there are important reservations, the results suggest extensive differences in the proteins of rat and chicken liver ribosomes. Despite those differences, rat and chicken liver ribosomal proteins maintain some homologous sequences present in bacterial ribosomal proteins. An enriched antibody preparation against chicken 80 S ribosomes inhibited the poly(U)-directed synthesis of polyphenylalanine and the elongation factor G (EF-G)-catalyzed binding of [3H]GDP to Escherichia coli ribosomes. Thus, chicken liver ribosomes, like ribosomes from rat liver and yeast, must have proteins homologous with those of E. coli ribosomes. (+info)Performance and specificity of monoclonal immunoassays for cyclosporine monitoring: how specific is specific? (2/4669)
BACKGROUND: Immunoassays designed for the selective measurement of cyclosporin A (CsA) inadvertently show cross-reactivity for CsA metabolites. The extent and clinical significance of the resulting overestimation is controversial. A comprehensive assessment of old and new methods in clinical specimens is needed. METHODS: In a comprehensive evaluation, CsA was analyzed in 145 samples with the new CEDIA assay and compared with the Emit assay with the old and new pretreatments, the TDx monoclonal and polyclonal assays, the AxSYM, and HPLC. All samples were from patients with liver and/or kidney transplants. RESULTS: The CEDIA offered the easiest handling, followed by the AxSYM, which showed the longest calibration stability. The TDx monoclonal assay provided the lowest detection limit and the lowest CVs. The mean differences compared with HPLC were as follows: Emit, 9-12%; CEDIA, 18%; AxSYM, 29%; and TDx monoclonal, 57%. The CycloTrac RIA paralleled the Emit results. In contrast to the mean differences, substantial (>200%) and variable overestimations of the CsA concentration were observed in individual patient samples. Metabolic ratios, estimates of the overall concentrations of several cross-reacting metabolites (nonspecific TDx polyclonal/specific reference method), correlated with the apparent biases of the various monoclonal assays. Metabolic ratios varied up to 10-fold, which translated into biases for individual samples between -7% and +174%. The higher the cross-reactivity of an assay was, the higher was the range of biases observed. The interindividual differences markedly exceeded other factors of influence (organ transplanted, hepatic function). CONCLUSION: Because assay bias cannot be predicted in individual samples, substantially erratic CsA dosing can result. The specificity of CsA assays for parent CsA remains a major concern. (+info)Automated homogeneous immunoassay for gentamicin on the dimension clinical chemistry system. (3/4669)
BACKGROUND: Monitoring of the concentration of gentamicin in serum and plasma during therapy is widely recommended and practiced in hospitals. Our aim was to develop a homogeneous immunoassay based on particle-enhanced turbidimetric inhibition immunoassay technology to quantify gentamicin on the Dimension clinical chemistry system. METHODS: Assay performance was assessed on each of the Dimension models in a 15-instrument interlaboratory comparison study. A split-sample comparison (n = 1171) was also performed between the gentamicin methods on the Dimension system and the Abbott TDx analyzer, using multiple reagent and calibrator lots on multiple instruments. RESULTS: The Dimension method was linear to 25.1 micromol/L (12.0 microg/mL) with a detection limit of 0.63 micromol/L (0.3 microg/mL). Calibration was stable for 30 days. The within-run imprecision (CV) was <1.3%, and total imprecision ranged from 1.8% to 3.2% between 4.2 micromol/L (2.0 microg/mL) and 16.7 micromol/L (8.0 microg/mL) gentamicin. Linear regression analysis of the results on the Dimension method (DM) vs the Abbott TDx yielded the following equation: DM = 0.98TDx - 0.42; r = 0.987. Minimal interference was observed from structurally related compounds such as sagamicin, netilmicin, and sisomicin. CONCLUSION: The monoclonal antibody used in this method has similar reactivities toward the individual gentamicin subspecies C1, C1a, and C2, thus providing analytical recovery not significantly dependent on relative subspecies concentrations. (+info)Serum IGF-binding protein-6 and prostate specific antigen in breast cancer. (4/4669)
OBJECTIVE: Recent studies have demonstrated the presence of the IGF-binding proteins (IGFBPs) and prostate specific antigen (PSA), an IGFBP protease. in human breast tissue. We sought to investigate the differences in serum IGFs, IGFBP-1, -3 and -6, and PSA between patients with surgically proven breast cancer and patients with benign breast disease. DESIGN AND METHODS: Concentrations of IGFs, IGFBP-1, -3 and -6, and PSA were determined in the sera from 57 patients with breast cancer (CA), and 46 women with benign breast disease (BBD) using immunoassays for IGFs and IGFBPs and an ultrasensitive ELISA for PSA. RESULTS: The mean (+/- S.E.M.) serum IGFBP-6 level in the CA group, 127 (16) ng/ml, was statistically significantly lower than in the BBD group, 157 (10) ng/ml (P = 0.016). Patients with CA had an elevated geometric mean serum PSA level of 0.018 (range: 0.0015-0.107) ng/ml, compared with 0.007 (range: 0.0015-0.019) ng/ml in women with BBD (P = 0.025). Mean serum IGFBP-1 concentrations were significantly lower in the CA group, 16 (2) ng/ml, versus 37 (4) ng/ml in the BBD group (P = 0.001). Mean serum IGFBP-3 concentrations were also lower in the CA group versus the BBD group, at 1981 (65) ng/ml, versus 2603 (140) ng/ml (P = 0.002) respectively. In the CA group, statistically significant correlations between PSA and IGFBP-6 (r = 0.413; P = 0.001), and between PSA and IGFBP-1 (r = -0.329; P = 0.021) were seen. Differences in IGF-I and -II between the two groups were not statistically significant. CONCLUSION: Lower serum concentrations of IGFBP-6, -3 and -1, but higher PSA concentrations were seen in the breast cancer group, and collectively these would suggest that there is an increase in bioavailable IGF-I in breast cancer. (+info)Differential immunodiagnosis between cystic hydatid disease and other cross-reactive pathologies. (5/4669)
We assessed an Echinococcus granulosus hydatid fluid antigen-ELISA (EgHF-ELISA) as a serologic prescreening test for E. granulosus infections, supplemented by more specific confirmatory tests, including arc-5 immunoprecipitation and antigen B subunit 8-kD immunoblotting. The diagnostic sensitivity of the EgHF-ELISA was 91%. With regard to the test specificity of the EgHF-ELISA (overall = 82%), we observed relatively frequent cross-reactions in tumor patients (6%) and in patients with other parasitic diseases. Cestode-related cross-reactivity can be resolved by the complementary use of E. multilocularis-specific antigens or Taenia solium cysticercosis-specific immunoblotting. Immunoblotting based upon the detection of antibody reactivity to the 8-kD antigen of EgHF, or if appropriately detectable, to the 29-kD and 34-kD bands exhibited a 91% diagnostic sensitivity and an overall specificity of 97% or 94%, respectively. Thus, immunoblotting provided a 99% discrimination between seropositive pre-operative cystic hydatid disease cases and cross-reactive non-cestode parasitic infections or malignancies. (+info)An immunoluminometric assay for N-terminal pro-brain natriuretic peptide: development of a test for left ventricular dysfunction. (6/4669)
Measurement of plasma levels of brain natriuretic peptide (BNP) has been used to assess left ventricular dysfunction and prognosis. Levels of the N-terminus of the precursor of BNP (NT-proBNP) have been reported to be elevated to a greater extent than BNP in left ventricular dysfunction. We have devised a non-radioactive sensitive and specific assay for NT-proBNP based on a competitive ligand binding principle. The chemiluminescent label 4-(2-succinimidyloxycarbonylethyl)phenyl-10-methylacridinium 9-carboxylate fluorosulphonate was used to label peptides representing domains in the middle and C-terminal sections of NT-proBNP. Assay of the C-terminal section of NT-proBNP (amino acids 65-76) in patients with proven left ventricular dysfunction [left ventricular wall motion index median 0.9 (range 0.3-1.4)] revealed elevated values [median 639 (386-911) fmol/ml] compared with normal controls [left ventricular wall motion index of 2 in all, NT-proBNP median 159 (120-245) fmol/ml, P<0.001]. Measurement of the middle section of NT-proBNP (amino acids 37-49) was not a discriminating test. It is thus possible to derivatize small peptides with a methyl acridinium label and preserve immunodetection with specific antibodies. Such methodology may allow non-radioactive immunoluminometric assays to be devised. (+info)Endometrial evaluation is not predictive for in vitro fertilization treatment. (7/4669)
PURPOSE: The main purpose of this study was to evaluate ovarian function by clomiphene citrate (CC) challenge test in a group of tubal infertile women and to study endometrial morphological maturation in the early luteal phase of CC-stimulated cycles as compared to in vitro fertilization (IVF) treatment outcome. METHODS AND RESULTS: Four women presented with strongly retarded, proliferative endometrium in the luteal phase. Of these, three presented with impaired ovarian function, high basal follicle-stimulating hormone, and high follicle-stimulating hormone levels after clomiphene stimulation on cycle day 10. In the remaining 30 women, showing an in-phase endometrium after CC stimulation, a comparison of six morphological characteristics did not reveal any significant differences between the 14 women who did become pregnant and the 16 who did not. No significant differences in endometrial thickness were observed between the groups. Significant differences were found when comparing estradiol and progesterone area under the curve during the luteal phase (P < 0.001 and P < 0.01, respectively) between those who did and those who did not become pregnant. CONCLUSIONS: Luteal endometrium morphology was not a sharp instrument to detect differences between women who did and women who did not become pregnant following IVF treatment, while ovarian function, as measured by hormonal markers, seemed to be a more reliable prognostic factor for IVF treatment outcome. (+info)Malignant transformation of human prostatic epithelium is associated with the loss of androgen receptor immunoreactivity in the surrounding stroma. (8/4669)
The cellular pathways involved in the pathogenesis of hormone resistance remain unclear. Studies evaluating the role of changes in human androgen receptor (hAR) expression in the progression of prostatic tumors have been inconclusive. Androgenic influence over prostatic growth is mediated via the regulation of interactions between stromal and epithelial cells. We hypothesized that neoplastic transformation of the prostate would be associated with alterations in hAR expression in the adjacent stroma. Using immunohistochemical techniques, we determined hAR positivity in the epithelium and adjacent stroma of sections from 17 benign and 39 malignant prostatic glands. We found that whereas the expression of the receptor decreased in both cellular compartments as the tissues dedifferentiated, the depletion was more pronounced in the stromal nuclei (P<0.0001). However, in sections from both untreated and hormone-resistant prostate cancer tissues, although heterogeneity of hAR expression in malignant epithelia was increased, there appeared to be a unique field effect around the cancerous prostate glands that resulted in a decreased expression of the receptor in the adjacent benign glands and its total loss in the surrounding stroma. The loss of hAR in the stroma adjacent to malignant prostatic epithelium may play an important role in prostate cancer progression. Furthermore, the similarity of the lack of stromal hAR expression in newly diagnosed and hormone-resistant prostate cancer (P = 0.85) may be an indication that the mechanisms responsible for the acquisition of hormone independence are established early in the malignant transformation process. (+info)
Lateral Flow Immunoassay
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Rapid and sensitive detection of 17β-estradiol in environmental water using automated immunoassay system with bacterial...
Studies towards automated ovulation prediction in the pig industry: an electrochemical immunosensor for oestradiol in saliva ...
Otitis Media - nthi
purchase of igg igm rapid test kites in malaysia - IgG/IgM Rapid Test Supplier
Production of monoclonal antibodies to ferritin and development of the enzyme immunoassay system<...
Patent US4361647 - Sandwich immunoassay and compositions for use therein - Google Patents
Emax Immunoassay Systems, Promega - Each
How to Buy Immunoassay Analyzers
US4581337A - Polyether polyamines as linking agents for particle reagents useful in immunoassays
- Google Patents
Antibody Detection Using Serological Assays- AAT Bioquest - Doron Scientific
Development of an Ultra-Sensitive Enzyme Immunoassay for Insulin and Its Application to the Evaluation of Diabetic Risk by...
ADVIA Centaur CP - Siemens Healthineers Bosna i Hercegovina
ADVIA Centaur XP - Siemens Healthineers Bosna i Hercegovina
CDIA™ Olaquindox Metabolite Colloidal Gold Test Cassette, Meat - Creative Diagnostics
Comparison of different cyclosporine immunoassays to monitor C<sub>0</sub> and C<sub>2</sub> blood levels from kidney...
ADVIA Centaur XP - Siemens Healthineers Österreich
CDIA™ Erythrosin Colloidal Gold Test Cassette, Soft Drinks - Creative Diagnostics
Human CGa clia kit | biobool.com
New paper-based test can help in early detection of HIV | The Siasat Daily
ALUXBIO CO., LTD. - Medical Diagnostic Manufacturer of Rapid Tests, Urinalysis
Immunoassay analyzer in work processing,analysis and diagnostics for ,allergy,anemia,cardiac,diabetes,growth ,hormone,hepatitis...
AIA-360 Automated Immunoassay Analyzer | https://www.diagnostics.us.tosohbioscience.com
Global Automated Immunoassay Analyzers: Ken Research
DIGITAL.CSIC: Site-heterologous haptens and competitive monoclonal antibody-based immunoassays for pyrimethanil residue...
Reveal® for Histamine | NEOGEN
cobas.com
Bronte Capital: A stock called Comedy: the joke is on someone or other
Fully automated chemiluminescence immunoassay of insulin using antibody-protein A-bacterial magnetic particle complexes. -...
Esoteric Testing Market to 2025 - Global Analysis and Forecasts by Technology (Chemiluminescence Immunoassay, Real-Time PCR,...
Dr. Rons Research Review - January 16, 2013
Patent US4280815 - Electrochemiluminescent immunoassay and apparatus therefor - Google Patents
A formulation science perspective applied to combination vaccines: particles-based multiplex immunoassays for improved antigens...
Surface-enhanced Raman spectroscopy
SERS-based immunoassay[edit]. SERS-based immunoassay is a novel technique for detection of low abundance biomarkers. Using ... Early detection of pancreatic cancer biomarkers was accomplished using SERS-based immunoassay approach.[43] This technology has ... "Multiplex detection of pancreatic cancer biomarkers using a SERS-based immunoassay". IOP Nanotechnology. Bibcode:2017Nanot.. ...
International unit
The unit count will often still remain in use.[clarify] Such a change has happened with the immunoassay standards for prolactin ... Ekins R (1991). "Immunoassay standardization". Scand J Clin Lab Invest Suppl. 205: 33-46. doi:10.3109/00365519109104600. PMID ... Wicher JT (1991). "Calibration is the key to immunoassay but the ideal calibrator is unattainable". Scand J Clin Lab Invest ...
Trinder glucose activity test
That Tjien Ngo (1988). Nonisotopic immunoassay. Plenum Press. p. 71. F. Yamagishi; T. Stanford; C. van Ast (2001). "Biosensors ...
Glycated hemoglobin
Immunoassay. *Boronate affinity chromatography. In the United States, HbA1c testing laboratories are certified by the National ...
Hook effect
An important practical relevance of the phenomenon is as a type of interference that plagues certain immunoassays and ... Tate, Jill; Ward, Greg (2004-05-01). "Interferences in Immunoassay". The Clinical Biochemist Reviews. 25 (2): 105-120. ISSN ... Miller, James (April 2004). "Interference in immunoassays: avoiding erroneous results" (PDF). CLI Online. CLI. Retrieved 3 ... Blocking antibody Schiettecatte, Johan; Anckaert, Ellen; Smitz, Johan (2012-03-23). "Interferences in Immunoassays". Advances ...
Human anti-mouse antibody
In the heterogeneous immunoassay the separation step will wash away the free substrate unable to bind due to HAMA blocking; ... Darwish, IA (2006). "Immunoassay Methods and their Applications in Pharmaceutical Analysis: Basic Methodology and Recent ... Tate, J; Ward, G (2004). "Interferences in immunoassay". Clin Biochem Rev. 25 (2): 105-20. PMC 1904417. PMID 18458713.. ... HAMA interferences can give false positive or negative immunoassay results. HAMA bridging interference produces artificially ...
Clearblue
Sarah Tiplady (2013). "7.3: Lateral Flow and Consumer Diagnostics". The Immunoassay Handbook. pp. 533-536. Retrieved 2013-10-17 ... "Chapter 7.4: Consumer Diagnostics-Clearblue Pregnancy and Fertility Testing". The Immunoassay Handbook. pp. 537-540. Retrieved ...
CSPD (molecule)
Kricka, Larry J (1993). "Ultrasensitive immunoassay techniques". Clinical Biochemistry. 26 (5): 325-331. doi:10.1016/0009-9120( ...
Antibody microarray
Furthermore, immunoassays on thiol-ene "synthetic paper" micropillar scaffolds have shown to generate a superior fluorescence ... Guo, W; Vilaplana, L; Hansson, J; Marco, P; van der Wijngaart, W (2020). "Immunoassays on thiol-ene synthetic paper generate a ... Silzel J. W., Cercek B., Dodson C., Tsay T., Obremski R. J. (1998). "Mass-sensing, multianalyte microarray immunoassay with ... Ekins RP (1989). "Multi-analyte immunoassay". J Pharm Biomed Anal. 7 (2): 155-68. doi:10.1016/0731-7085(89)80079-2. PMID ...
Cystatin C
A new avidin-biotin ELISA assay for its measurement". Journal of Immunoassay. 13 (1): 47-60. doi:10.1080/15321819208019824. ... using immunoassays such as nephelometry or particle-enhanced turbidimetry. It is a more expensive test than serum creatinine ( ... "Adult cystatin C reference intervals determined by nephelometric immunoassay". Clinical Biochemistry. 40 (13-14): 1084-7. doi: ...
Ara h1
Mills E, Potts A, Plumb G, Lambert N, Morgan M (1997). "Development of a rapid dipstick immunoassay for the detection of peanut ... In these tests, peanut allergens are measured in foods by immunoassay with human IgE antibodies. In 2003 a monoclonal antibody- ... Keating MU, Jones RT, Worley NJ, Shively CA, Yunginger JW (July 1990). "Immunoassay of peanut allergens in food-processing ... Yeung JM, Collins PG (1996). "Enzyme immunoassay for determination of peanut proteins in food products". Journal of AOAC ...
Proteomics
Examples of these methods are the MSIA (mass spectrometric immunoassay), developed by Randall Nelson in 1995, and the SISCAPA ( ... 1995). "Mass Spectrometric Immunoassay". Analytical Chemistry. 67 (7): 1153-1158. doi:10.1021/ac00103a003. ISSN 0003-2700. PMID ... Generally, proteins may be detected by using either antibodies (immunoassays) or mass spectrometry. If a complex biological ... The lower limit of detection with conventional immunoassay technology is the upper femtomolar range (10−13 M). Digital ...
Triad (environmental science)
... and immunoassay test kits. New field analytical methods are fast developing. However, some have used standard laboratory ... Immunoassay and Enzymatic Assays". "Technologies for Cleaning up Contaminated Sites" (PDF). 2015-01-27. http://www.triadcentral ...
Thyroid function tests
Method: Microparticle Enzyme Immunoassay. Beckman Coulter provides the equipment and reagents used in the 2009-2011 CDC manuals ... Method: Microparticle Enzyme Immunoassay. Lab 18 TSH - Thyroid Stimulating Hormone. 1999-2000. ...
AKR1B1
Immunoassay of human aldose reductase". Biochemical Pharmacology. 46 (1): 21-8. doi:10.1016/0006-2952(93)90343-U. PMID 8347133 ...
Digital microfluidics
Both heterogeneous immunoassays (antigens interacting with immobilized antibodies) and homogeneous immunoassays (antigens ... have been incorporated onto DMF immunoassay platforms. The incorporation of magnetic bead-based assays onto a DMF immunoassay ... Shamsi MH, Choi K, Ng AH, Wheeler AR (February 2014). "A digital microfluidic electrochemical immunoassay". Lab on a Chip. 14 ( ... The advanced fluid handling capabilities of digital microfluidics (DMF) allows for the adoption of DMF as an immunoassay ...
Bioassay
Assay Immunoassay Hoskins, W. M.; Craig, R. (1962-01-01). "Uses of Bioassay in Entomology". Annual Review of Entomology. 7 (1 ...
Erythroferrone
September 2017). "Immunoassay for human serum erythroferrone". Blood. 130 (10): 1243-1246. doi:10.1182/blood-2017-04-777987. ... Erythroferrone levels in blood have been shown by immunoassay to be higher after blood loss or erythropoetin administration. ...
Quidel Corporation
... fluorescent immunoassay products (Sofia); and (5) molecular diagnostic products. These products include assays such as QuickVue ... Quidel's core competencies and capabilities include immunoassay development, automated manufacturing, monoclonal antibody ...
Droplet-based microfluidics
Shamsi MH, Choi K, Ng AH, Wheeler AR (February 2014). "A digital microfluidic electrochemical immunoassay". Lab on a Chip. 14 ( ... magnetic microparticles in the fluids replaced the typical fluorescence-based detection of droplets in the immunoassay of ...
Photoacoustic effect
The photoacoustic immunoassay labels and detects target proteins using nanoparticles that can generate strong acoustic signals ... Zhao Y, Cao M, McClelland JF, Lu M (2016). "A photoacoustic immunoassay for biomarker detection". Biosensors and Bioelectronics ...
Oleandrin
Fluorescence polarization immunoassay is widely used. This test is slower and has a lower sensitivity than digoxin immunoassay ... and their detection by digoxin immunoassays". Clinical Chemistry. 42 (10): 1654-8. doi:10.1093/clinchem/42.10.1654. PMID ...
Salicylate testing
Another identification mechanism is through immunoassay. Abbott Labs' AxSYM is an immunoassay device utilizing Fluorescence ... Polarization Immunoassay (FPIA) technology that can determine the presence and quantify salicylates. The introduction of a ...
Potato virus Y
Use of enzymes in immunoassay techniques. Analyst, 109: 533-547. Gugerli, P. and Gehriger, W. (1980). Enzyme-linked ... Laboratory techniques in biochemistry and molecular biology practice and theory of enzyme immunoassays, volume 15, Elsevier ...
Photoacoustic spectroscopy
The photoacoustic immunoassay labels and detects target proteins using nanoparticles that can generate strong acoustic signals ... "A photoacoustic immunoassay for biomarker detection". Biosensors and Bioelectronics. 85: 261-66. doi:10.1016/j.bios.2016.05.028 ...
Optical fiber
Surround optical-fiber immunoassay (SOFIA). *XENPAK. References[edit]. *^ "Optical Fiber". www.thefoa.org. The Fiber Optic ...
Pregnancy test
In the 1970s, the discovery of monoclonal antibodies led to the development of the relatively simple and cheap immunoassays, ... Yalow RS, Berson SA (July 1960). "Immunoassay of endogenous plasma insulin in man". Journal of Clinical Investigation. 39 (7): ...
Stable isotope standards and capture by anti-peptide antibodies
2007 Dec 12;605(1):70-9. Nelson R, Krone J, Bieber A. Mass spectrometric immunoassay. Anal Chem. 1995 Ackermann BL. Hybrid ... Serum thyroglobulin: a model of immunoassay imperfection. 2006;(8):12-4. Hoofnagle AN, Roth MY. Improving the Measurement of ... "mass spectrometric immunoassay",) in which antibodies are used to enrich target proteins, which are analyzed intact by MS; and ... including conventional immunoassays (e.g., ELISA assays). Elimination of such autoantibody interferences drove the adoption of ...
Abbott AxSYM
Evaluation of the Abbott AxSYM Immunoassay Analyser - Agnes D'Souza, M. J. Wheeler, Great Britain. Medical Devices Agency (6S)- ... Smith, J; Osikowicz, G (October 1993). "Abbott AxSYM random and continuous access immunoassay system for improved workflow in ... Evaluation of the Abbott AxSYM immunoassay analyser. Medical Devices Agency. 1996. ISBN 9781858395388. ...
Solomon Berson
Yalow, RS; Berson, SA (1960). "Immunoassay of endogenous plasma insulin in man". J Clin Invest. 39 (7): 1157-75. doi:10.1172/ ...
Mass spectrometric immunoassay - Wikipedia
Mass spectrometric immunoassay (MSIA) is a method that uses antibodies to analyze protein differences in biological samples.[1] ... 1995). "Mass Spectrometric Immunoassay". Analytical Chemistry. 67 (7): 1153-1158. doi:10.1021/ac00103a003. ISSN 0003-2700. PMID ... Retrieved from "https://en.wikipedia.org/w/index.php?title=Mass_spectrometric_immunoassay&oldid=758985804" ...
Immunoassay | Encyclopedia.com
Definition Immunoassays are chemical tests used to detect or quantify a specific substance, the analyte, in a blood or body ... Law, B. Immunoassay: A Practical Guide. London: Taylor and Francis, 1996.. Wild, D., ed. Immunoassay Handbook, 2nd Ed. London: ... Chemiluminescent immunoassay- An immunoassay in which the label is a molecule that emits light when excited by a chemical ... Enzyme immunoassay- An immunoassay using an enzyme as the label. The enzymatic reaction product is measured to determine the ...
Immunoassay analyser
Patent US5942407 - Light-emitting immunoassay - Google Patents
Migratory detection immunoassay. US4978502 *. Dec 15, 1987. Dec 18, 1990. Dole Associates, Inc.. Immunoassay or diagnostic ... Three Layered Dry Phase Immunoassay Device. A light emitting immunoassay device in accordance with the present invention was ... The immunoassay devices are preferably held or positioned by use of a holding assembly such as a test strip holder known in the ... Multi-spot enzyme immunoassay method. US4818677 *. Dec 3, 1987. Apr 4, 1989. Monoclonal Antibodies, Inc.. Membrane assay using ...
Patent US4495151 - Element for immunoassay - Google Patents
A thin layer of constant thickness of a liquid specimen for immunoassay is thus receivable between the complementary surfaces. ... Apparatus for performing an immunoassay, comprises in combination a reaction tube closed at its bottom, and in the reaction ... A further object of the present invention is to provide an element for immunoassay which can perform immunoassay with a small ... Solid phase immunoassay with carriers matching the shape of sample wells. US6207463. 20 Oct 1995. 27 Mar 2001. Labsystems Oy. ...
BBiomedicina | Immunoassay | Elisa
horseradish peroxidase and b-galatosidase.Enzyme Immunoassay (EIA) • In enzyme immunoassays (EIA). ... Immunoassays that require separation of the bound Ab-Ag* complex are referred to as being heterogeneous immunoassays. • ... Those that do not require separation are referred to as homogeneous immunoassays.Heterogeneous and Homogeneous Immunoassays ... Immunoassays use one or more select antibodies to detect analytes of interest. ...
Immunoassay
Immunoassays are analytical techniques that are widely used to detect and quantitate the concentration of a protein, metabolite ... Immunoassays are analytical techniques that are widely used to detect and quantitate the concentration of a protein, metabolite ... Homogeneous immunoassays do not require a physical separation step.. *Antigens present in excess concentrations can produce ... Immunoassays are also the mainstays in research, for example, understanding the pathophysiology of medical disorders in order ...
Patent US4849338 - Simultaneous calibration heterogeneous immunoassay - Google Patents
Membrane-based immunoassay method. US5432057 *. 28 Aug 1992. 11 Jul 1995. Syva Company. Simultaneous calibration heterogeneous ... Simultaneous calibration heterogeneous immunoassay. US5354692 *. 8 Sep 1992. 11 Oct 1994. Pacific Biotech, Inc.. Analyte ... Simultaneous calibration heterogeneous immunoassay. US5308775 *. 15 Sep 1992. 3 May 1994. Abbott Laboratories. Assay devices ... Simultaneous calibration heterogeneous immunoassay. US4540659 *. 16 Jul 1982. 10 Sep 1985. Syva Company. Simultaneous ...
Patent US4177253 - Magnetic particles for immunoassay - Google Patents
Immunoassay using magnetic particle. EP0420186A3 *. Sep 26, 1990. Jul 31, 1991. Fujirebio Inc.. Immunoassay using magnetic ... Metal sol particle immunoassay. US4342739 *. Jan 8, 1980. Aug 3, 1982. Fuji Photo Film Co., Ltd.. Novel material for ... Laser magnetic immunoassay method and method by a magnetophoresis apparatus therefor. US5238811 *. Apr 27, 1992. Aug 24, 1993. ... A method of immunoassay comprising the steps of reacting an antigen with an antibody, one of which is the biologically active ...
Measuring Neutrophil Elastase With ProteaseTag Immunoassay
Quantitative Measurement of Active Neutrophil Elastase Levels Using ProteaseTag™ Active NE Immunoassay. *Download PDF Copy ... Active NE is captured using the NE-Tag in the ProteaseTag™ Active NE Immunoassay (Figure 1). A following antibody step allows ... ProteaseTag™ Active Neutrophil Elastase Immunoassay (PA001) from ProAxsis. Before starting the assay, samples of sputum were ... On binding, they bridge a solid support, facilitating their incorporation into a format of immunoassay for particular ...
Chromogenic Substrates for Enzyme Immunoassay | SpringerLink
... described according to their kinetics and structure only a few have turned out to be marker enzymes in the enzyme immunoassay ( ... Porstmann B., Porstmann T. (1988) Chromogenic Substrates for Enzyme Immunoassay. In: Ngo T.T. (eds) Nonisotopic Immunoassay. ... 5-tetramethylbenzidine as an Ames test negative chromogen for horse-radish peroxidase in enzyme-immunoassay, J. of Immunoassay ... Cox, C., 1983, NAD labeled immunoassays, Trends in Anal. Chem., 2: 129-131.CrossRefGoogle Scholar ...
Web Industries Opens New Lateral Flow Immunoassay (LFI) Manufacturing Facility
Lumit™ Immunoassay Cellular Systems
Immunoassay Cellular System is a no-wash bioluminescent immunoassay that measures levels of target analytes in cell lysates ... Lumit™ Immunoassay vs. Western Blot Lumit™ Immunoassays are easier and much faster than traditional Western blots while ... The Lumit™ Immunoassay Cellular System is a no-wash bioluminescent immunoassay that measures target analytes directly in cell ... How the Lumit™ Immunoassay Cellular System Works Lumit™ Immunoassay Cellular Systems include Lumit™ Secondary Antibodies ( ...
Immulite Chemiluminescent Immunoassay System
Worldwide, IMMULITE systems have been chosen for various types of immunoassay testing due to their flexible platforms and ... The IMMULITE chemiluminescent immunoassay system is designed for ease of use, reliability, high-performance assays and fully ... Access Immunoassay System. 8. Access 2 Immunoassay System. 9. ADVIA Centaur Immunoassay System. 10. AIA - 600 II Immunoassay ... Immulite 1000 Immunoassay System. 2. Immulite 2000 Advanced Immunoassay System. 3. Immulite 2500 SMS. 4. Immulite PAP. 5. ...
Immunoassay and Immunology Controls
R&D Systems™ Quantikine™ Immunoassay Control Set 919 for Human Total IL-18. Immunoassay control set for Human Interleukin 18 ... R&D Systems™ Quantikine Immunoassay Control Group 10 Control for Human High Sensitivity IL-1 beta, IL-4, IL-7, IL-8, IL-10, IL- ... R&D Systems™ Quantikine Immunoassay Control Group 8 Control for Human CXCL6/GCP-2, CXCL8/IL-8, CXCL10/IP-10, CXCL11/I-TAC, ... R&D Systems™ Quantikine Immunoassay Control Group 9 Control for Human AgRP, Carbonic Anhydrase IX, CD23/Fc epsilon RII, FGF-19 ...
Osteocalcin by Electrochemiluminescent Immunoassay
... ,ARUP Laboratories is a national reference laboratory and a worldwide leader ... Access Immunoassay System. 4. Access 2 Immunoassay System. 5. ADVIA Centaur Immunoassay System. 6. AIA - 600 II Immunoassay ... AIA - 360 Immunoassay Analyzer. 8. AIA - 1800 Immunoassay Analyzer. 9. Immulite Chemiluminescent Immunoassay System. 10. ...
Immunoassay - HORIBA
Immunoassay - Wikipedia
Multi-step assays are often called separation immunoassays or heterogeneous immunoassays. Some immunoassays can be carried out ... Immunoassays can be run in a number of different formats. Generally, an immunoassay will fall into one of several categories ... Immunoassays come in many different formats and variations. Immunoassays may be run in multiple steps with reagents being added ... "The Immunoassay Handbook", 3rd Edition, David Wild, Ed., Elsevier,2008 Immunoassay at the US National Library of Medicine ...
Calibrator for NT-proBNP immunoassay - ALVI AZHAR
... method to efficiently express high levels of a recombinant untagged NT-proBNP for use as a calibrator in NT-proBNP immunoassays ... In such immunoassays, the purified analyte is also the calibration material. (see The Immunoassay Handbook, edited by David ... protein is capable of functioning as a calibrator in immunoassays specific for NT-proBNP such as those immunoassays disclosed ... 3. The purified NT-BNP of claim 2 capable of functioning as a calibrator in an immunoassay specific for NT-BNP. 4. A series of ...
Designing a Multiplex Immunoassay: Factors to Consider
Some of the major factors to consider when designing a highly parallel multiplex immunoassay are variability, sample collection ... Variability between immunoassay kits. Multiplex immunoassay kits, even from the same manufacturer, may not give identical ... Multiplex immunoassays and enzyme-linked immunosorbent assays (ELISA) are used to determine and analyze the presence of an ... Multiplex immunoassays can be differentiated by the surface to which the antigens or antibodies are attached. These surfaces ...
Cytoxan antibodies and immunoassay - Saladax Biomedical Inc.
The immunoassay of claim 9, wherein the sample is taken from a human. 12. The immunoassay of claim 11, wherein the antibody is ... 2. The immunoassay of claim 1, wherein the sample is a human sample. 3. The immunoassay of claim 2, wherein the antibody is ... 4. The immunoassay of claim 3, wherein the antibody is attached to a solid support. 5. The immunoassay of claim 4, wherein the ... 8. The immunoassay of claim 7, wherein R7 is 2,4-dinitrophenyl. 9. The immunoassay of claim 3, wherein the protecting group is ...
Automating and Miniaturizing Immunoassays
Switching to an automated immunoassay platform. Switching from an ELISA format to Gyrolab immunoassay technology should not ... Home Magazine BioPerspectives Automating and Miniaturizing Immunoassays: Continuing the Progress to Maximum Productivity ... Transferring immunoassays from traditional standard formats such as ELISA onto an automated platform like Gyrolab offers ... Miniaturizing immunoassays can deliver tremendous savings in sample volumes, animal use, and reagent consumption. Although ...
Enzyme immunoassay - Wikipedia
Patent US7550267 - Microscale diffusion immunoassay utilizing multivalent reactants - Google Patents
An improved microscale diffusion immunoassay utilizing multivalent reactants is disclosed. In particular, a method for ... Microscale diffusion immunoassay. US20020090644. 14 Feb 2000. 11 Jul 2002. Weigl Bernhard H.. Microscale diffusion immunoassay ... The present invention relates to immunoassays, and, more particularly, to improved microscale diffusion immunoassays utilizing ... Flow injection immunoassays have taken advantage of specific flow conditions (see U. de Alwis and G. S. Wilson, Anal. Chem. 59 ...
Immunoassay Market worth $23.7 Billion by 2019: MarketsandMarkets
Browse 352 market data tables and 40 figures spread through 305 pages and in-depth TOC on "Immunoassay Market" ... This segment is also expected to grow at the highest CAGR during the forecast period, owing to the increase in immunoassay ... Based on region, the global immunoassay market is dominated by North America, followed by Europe, Asia, and the Rest of the ... In Vitro Diagnostic (IVD) Market [Instruments, Reagents & Data Management Systems] [Technique (Immunoassay, Clinical Chemistry ...
Immunoassay and Other Bioanalytical Techniques - 1st Edition - Jeanett
Patent US5395754 - Membrane-based immunoassay method - Google Patents
The present invention is directed to a membrane-based immunoassay method for an analyte of interest having at least two ... Immunoassay, 13 127-131 (1990).. 4. Bangs, L., "New Developments In Particle-Based Tests and Immunoassays," J. Int. Fed. Clin. ... Immunoassay, 13 127 131 (1990).. 8. *. Bangs, L., New Developments In Particle Based Tests and Immunoassays, J. Int. Fed. Clin ... "Latex Immunoassays," J. Clin. Immunoassay, 13 127-131 (1980) both of which are incorporated herein by reference. Various ...
Immunoassays for drug screening in urine | SpringerLink
Immunoassays are presently used worldwide for the rapid screening of drugs. Despite the fact that they are a highly valuable ... Schütz H. Drogenscreening mit Immunoassays. Pharmazie in unserer Zeit 1999;28:320-328.PubMedCrossRefGoogle Scholar ... Immunoassays are presently used worldwide for the rapid screening of drugs. Despite the fact that they are a highly valuable ... Effect of glutaraldehyde (Urin Aid) on detection of abused drugs in urine by immunoassay. Clin Chem 1994;40:1605, 1606.PubMed ...
Analysis of Automated Immunoassay Analyzers Market
... applications and companies active in the industry , Biokit, Carolina Liquid ... Analysis of Automated Immunoassay Analyzers Market applications and companies active in the industry , Biokit, Carolina Liquid ... The emergence of automation in the medical sector is one of the many drivers that will fuel automated immunoassay analyzers ... India is one of those regions that is likely to observe exponential growth in automated immunoassay analyzers market. In India ...
Immunoassay - Related Articles - Citizendium
See also changes related to Immunoassay, or pages that link to Immunoassay or to this page or whose text contains "Immunoassay" ... Immunosorbent technique [r]: Sensitive immunoassay that uses an enzyme linked to an antibody or antigen as a marker for the ... Retrieved from "http://en.citizendium.org/wiki?title=Immunoassay/Related_Articles&oldid=100394252" ...
AssayAnalyzerReagentsFlow immunoassayHeterogeneous immunoassaysAntigensAnalytePlayers in the immunoassay marketMarket is segmentedQuantitativeImmulite Chemiluminescent Immunoassay SystemDiagnosticsDiagnosisSolid phase immunoassaysMass Spectrometric ImmunoassayEnzyme-linked immunosorbent assaysReagentAnalytesAnalyticalAnalyzersAssays and immunoassaysMSIAHomogeneous immunoassayMultiplex immunoassayCompetitive immunoassayGlobal chemiluminescenceChemiluminescence ImmunoassayAnalyserBiomarkersElectrochemicalProteinConjugatesRadioimmunoassayDetect2016ColorimetricQuantificationSpecificitySerumAntibody DetectionProteinsInvention relatesMeasurementCommercially availableDynamicsInterferenceLaboratory
Assay18
- An example of a qualitative assay is an immunoassay test for pregnancy. (encyclopedia.com)
- The purpose of an immunoassay is to measure (or, in a qualitative assay, to detect) an analyte. (encyclopedia.com)
- The molecule detected by the immunoassay is often referred to as an "analyte" and is in many cases a protein, although it may be other kinds of molecules, of different sizes and types, as long as the proper antibodies that have the required properties for the assay are developed. (wikipedia.org)
- Immunoassays may be run in multiple steps with reagents being added and washed away or separated at different points in the assay. (wikipedia.org)
- Enzyme Linked Immunosorbent Assay (ELISA) is an application of solid phase heterogeneous sandwich immunoassay that combines enzyme-antibody label reagent with a solid phase bound antibody. (scribd.com)
- These may include: Enzyme-linked immunosorbent assay (ELISA) Enzyme multiplied immunoassay technique (EMIT) This article includes a list of related items that share the same name (or similar names). (wikipedia.org)
- It shows (a) how miniaturizing immunoassays can deliver significant savings in sample volumes and (b) how the Gyrolab platform secures smooth assay/method transfer between key entities such as pharma companies, CROs, and CMOs, even those on different continents. (genengnews.com)
- Although reducing assay volumes in manual ELISA immunoassays is possible, it is tedious to carry out and challenging to maintain assay performance. (genengnews.com)
- ELISA method development and the design of our assay validations are tailored to meet both client and regulatory requirements, and we are experienced in working with clients on the generation and qualification of critical immunoassay reagents. (criver.com)
- On the basis of technique the market is segmented into enzyme-linked immunosorbent assay (ELISA), Radioimmunoassay (RIA), chemiluminescence immunoassays (CLIA) and others. (openpr.com)
- Diagnostic accuracy of the immunoassay was assessed against a whole spike protein-based recombinant immunofluorescence assay (rIFA) by receiver operating characteristic (ROC) analyses. (nih.gov)
- To find the best assay for your needs, choose from popular immunoassay categories below or contact our field application specialists (FAS) , who can answer your questions and provide you with individualized immunoassay support, including information about new immunoassay technologies. (thermofisher.com)
- All our immunoassay systems feature standardized test menus, assay protocols, instrument processes and reference ranges. (beckmancoulter.com)
- During development, an immunoassay, including components and individual assay steps, goes through many levels of screening to ensure that it accurately and reproducibly measures the target protein of interest, with stringent quality control measures to ensure consistency over time. (rndsystems.com)
- the most common types are enzyme-linked immunosorbent assay (ELISA) and enzyme-multiplied immunoassay technique (EMIT). (thefreedictionary.com)
- Enzyme immunoassay (EIA) and enzyme-linked immunosorbent assay (ELISA) have become household names for medical laboratories, manufacturers of in vitro diagnostic products, regulatory bodies, and external quality assessment and proficiency-testing organizations. (thefreedictionary.com)
- The BED IgG-Capture Enzyme Immunoassay (BED assay) is a commercially available and widely used test for recent HIV infection. (nih.gov)
- The assay is designed for use on all Roche immunoassay systems for low-, mid- and high-volume testing environments. (rttnews.com)
Analyzer9
- This new immunoassay was built for use with the Cobas e601 analyzer. (uspharmacist.com)
- The purpose of this study is to demonstrate the ability of the Strep A Fluorescent Immunoassay Analyzer to accurately detect a throat swab specimen for the presence or absence of Strep A when compared to culture. (clinicaltrials.gov)
- This benchtop immunoassay analyzer fits labs with space constraints and delivers fast, reliable results. (beckmancoulter.com)
- This immunoassay analyzer gives technologists the benefit of ease of use without sacrificing throughput. (beckmancoulter.com)
- ResearchMoz added Latest Research Report titled ' Global Chemiluminescence immunoassay analyzer Industry 2015: Market Forecasts for Liquefied Natural Gas (LNG), Oil Storage, Pipelines and Gas Processing ' to it's Large Report database. (sbwire.com)
- Albany, NY -- ( SBWIRE ) -- 02/08/2016 -- The Global Chemiluminescence immunoassay analyzer Industry Report 2015 is a professional and in-depth study on the current state of the Chemiluminescence immunoassay analyzer industry. (sbwire.com)
- The Chemiluminescence immunoassay analyzer market analysis is provided for the international markets including development trends, competitive landscape analysis, and key regions development status. (sbwire.com)
- The Chemiluminescence immunoassay analyzer industry development trends and marketing channels are analyzed. (sbwire.com)
- The Freedom EVOlyzer is a new enzyme immunoassay (EIA) analyzer that offers fully automated microplate processing. (thefreedictionary.com)
Reagents20
- Some immunoassays can be carried out simply by mixing the reagents and sample and making a physical measurement. (wikipedia.org)
- Radioactive isotopes can be incorporated into immunoassay reagents to produce a radioimmunoassay (RIA). (wikipedia.org)
- SmartAssay test kits are complete immunoassays for analyzing pesticide residue, including all required reagents. (horiba.com)
- Novel conjugates of protected aldehyde active metabolites of cyclophosphamide including reagents and immunogens thereof and monoclonal antibodies generated by these immunogens, said reagents and immunogens useful in immunoassays for the monitoring of the active metabolites of cyclophosphamide in patients being treated with cyclophosphamide. (freepatentsonline.com)
- Among the various products and services available, the reagents and kits segment accounted for the largest share of the immunoassay market in 2014. (pitchengine.com)
- This segment is also expected to grow at the highest CAGR during the forecast period, owing to the increase in immunoassay tests conducted due to rising aging population, rising number of chronic diseases, increased variety of reagents and the analytical techniques employed, trends like the high-growth at-home testing market, and accessibility to a wide range of reagents. (pitchengine.com)
- In Vitro Diagnostic (IVD) Market [Instruments, Reagents & Data Management Systems] [Technique (Immunoassay, Clinical Chemistry, Molecular Diagnostics, Haematology) & Applications (Diabetes, Infectious Diseases, Cancer & Cardiology)] Systems, End Users] - Forecast To 2017. (pitchengine.com)
- Global Immunoassay Reagents and Devices Market accounted to USD 16.25 billion in 2016 growing at a CAGR of 7.8% during the forecast period of 2017 to 2024. (openpr.com)
- The global immunoassay reagents market is segmented on the basis of product type, technique, application, end-user, and geography. (openpr.com)
- On the basis of product type the global immunoassay reagents market is segmented into reagents, analyzers, software and services. (openpr.com)
- On the basis of application the global immunoassay reagents markt is segmented into infectious diseases, endocrinology, cardiology, oncology and others. (openpr.com)
- The Global Immunoassay Reagents and Devices Market Report contains an in-depth profiling of the key market players, along with the recent developments (new product launches, partnerships, agreements, collaborations, and joint ventures) and strategies adopted by them to sustain and strengthen their positions in the market. (openpr.com)
- The stringent regulatory procedures for the approval of the immunoassay products and the technological hurdles of the immunoassay kits and reagents have impacted the market. (marketsandmarkets.com)
- Also, the technological hurdles posed by the immunoassay kits and reagents such as cross-reactivity of the antibodies, low detection limits of the available antibodies, false negatives and others decelerates the growth of the immunoassay market. (marketsandmarkets.com)
- Reagents & kits segment dominated the immunoassay market in 2020. (marketsandmarkets.com)
- Based on product, the immunoassay market is segmented into reagents & kits and analyzers. (marketsandmarkets.com)
- Reagents & kits account for the largest share of the immunoassay market and is expected to grow at the highest CAGR. (marketsandmarkets.com)
- In 2017, the reagents and kits segment accounted for the largest share of the immunoassays market. (webnewswire.com)
- GenScript offers a broad range of protein detection and immunoassay products, including ONE-HOUR Western™ Detection System, Express™ ELISA Kits, Protein A ELISA Kit, cell lys ate products and related reagents for protein analysis that support your research. (genscript.com)
- This guideline addresses components for harmonizing and assessing the quality of immunoassay systems for several commonly used dose-response indicator categories, e.g., radioisotopes, enzymes, fluorescence, luminescence, reagents, and experimental components criteria essential to characterizing an immunoassay. (clsi.org)
Flow immunoassay6
- MARLBOROUGH, Mass. , April 28, 2016 /PRNewswire/ -- Web Industries, Inc., innovative provider of precision contract manufacturing and converting services, is proud to announce the opening of the company's first lateral flow immunoassay (LFI) production facility. (prnewswire.com)
- I think 'Multiplex Flow Immunoassay' simply refers to the scientific method used in performing an ANA test. (healingwell.com)
- Lateral Flow Immunoassay for Rapid Detection of Grapevine Leafroll-Associated Virus. (harvard.edu)
- To further verify the result, we developed a multi-target lateral flow immunoassay strip (msLFIA) with staking pad design to obtain on-site rapid response for clinical diagnosis of PJI. (nature.com)
- In addition, we developed a multi-target lateral flow immunoassay strip (msLFIA) with staking pad design to obtain on-site rapid response for clinical diagnosis of PJI, which the multi-target design is used to increase specificity and the stacking pad design is to enhance detection sensitivity. (nature.com)
- Web Industries, Inc., a provider of precision contract manufacturing and converting services, opened the company's first lateral flow immunoassay production facility co-located within the company's existing location in Boston, Massachusetts . (areadevelopment.com)
Heterogeneous immunoassays5
- Multi-step assays are often called separation immunoassays or heterogeneous immunoassays. (wikipedia.org)
- Immunoassays that require separation of the bound Ab-Ag* complex are referred to as being heterogeneous immunoassays. (scribd.com)
- Heterogeneous immunoassays require a physical separation between antibody‐bound and free analytes. (els.net)
- Schematic representation of competitive limited‐reagent (a) and immunometric excess‐reagent (b) heterogeneous immunoassays. (els.net)
- Photophysics of Heterogeneous Immunoassays (O.S. Khahil). (booktopia.com.au)
Antigens9
- Qualitative immunoassays are often used to detect antigens on infectious agents and antibodies that the body produces to fight them. (encyclopedia.com)
- For example, immunoassays are used to detect antigens on Hemophilus, Cryptococcus , and Streptococcus organisms in the cerebrospinal fluid (CSF) of meningitis patients. (encyclopedia.com)
- Most, though not all, immunoassays involve chemically linking antibodies or antigens with some kind of detectable label. (wikipedia.org)
- Immunoassays employ a variety of different labels to allow for detection of antibodies and antigens. (wikipedia.org)
- An immunoassay is a test that uses immunocomplexing when antibodies and antigens are brought together. (scribd.com)
- Multiplex immunoassays can be differentiated by the surface to which the antigens or antibodies are attached. (news-medical.net)
- For the detection and quantitation of antibodies or antigens an immunoassay has to fulfill three basic requirements: highly specific, highly sensitive, and reproducible. (springer.com)
- Antigen Quantification: Measurement of Multivalent Antigens by Solid-Phase Immunoassay (R.G. Hamilton). (booktopia.com.au)
- RSV antigens were detected by rapid enzyme immunoassay in the nasal aspirates of 6. (thefreedictionary.com)
Analyte8
- Immunoassays are chemical tests used to detect or quantify a specific substance, the analyte, in a blood or body fluid sample, using an immunological reaction. (encyclopedia.com)
- In other words, in some immunoassays, the analyte may be an antibody rather than an antigen. (wikipedia.org)
- Multiplex immunoassays and enzyme-linked immunosorbent assays (ELISA) are used to determine and analyze the presence of an analyte in a sample through the binding of complimentary antigen-antibody pairs. (news-medical.net)
- The present invention is directed to a membrane-based immunoassay method for an analyte of interest having at least two sterically separate antigenic sites. (google.com.au)
- Our microfluidic method facilitates hands-free saliva analysis by integrating sample pretreatment (filtering, enrichment, mixing) with electrophoretic immunoassays to quickly measure analyte concentrations in minimally pretreated saliva samples. (pnas.org)
- The invention concerns a method for the detection of an analyte in a sample liquid by an enzyme-immunoassay in which an enzyme-labelled compound is partitioned between a solid and a liquid phase and the amount of enzyme label in the liquid phase outside the solid phase is determined as a measure of the. (google.co.uk)
- While ELISA is still commonly used to characterise a single analyte, laboratories and organisations are moving towards multiplex immunoassays. (intechopen.com)
- Based on the sandwich immunoassay principle, a test strip was made of a nitrocellulose membrane dotted with monoclonal antibodies against VCO1 as analyte capture molecules. (hindawi.com)
Players in the immunoassay market1
- Key players in the immunoassay market include are Roche Diagnostics (Switzerland), Siemens Healthcare (Germany), Abbott Laboratories (U.S.), Beckman Coulter (U.S.), Ortho Clinical Diagnostics (U.S.), and bioMérieux (France). (pitchengine.com)
Market is segmented1
- On the basis of technology, the immunoassay market is segmented into ELISA, rapid tests, western blotting, enzyme-linked immunospot (ELISPOT), immuno-PCR, and other immunoassay technologies. (webnewswire.com)
Quantitative9
- Immunoassays may be qualitative (positive or negative) or quantitative (amount measured). (encyclopedia.com)
- Quantitative immunoassays are performed by measuring the signal produced by the indicator reaction. (encyclopedia.com)
- A newer approach to immunoassays involves combining real-time quantitative polymerase chain reaction (RT qPCR) and traditional immunoassay techniques. (wikipedia.org)
- cGMP Enzyme Immunoassay Kit has been used for the quantitative determination of cyclic guanine monophosphate (cGMP) concentration in parasite tissues and mouse aortic artery homogenates. (sigmaaldrich.com)
- Our multiplex immunoassays use the FirePlex ® particle technology to enable the quantitative measurement of many human proteins and peptides at the same time using as little as 12.5 μL of sample per well. (abcam.com)
- The microengraving immunoassay is based on intaglio printing, in which a glass slide is temporarily sealed to the array of nanowells to capture proteins secreted by confined cells in both a multiplexed and quantitative manner. (mdpi.com)
- OTCQX: RHHBY) today announced the launch of the Elecsys Anti-p53 immunoassay for the in vitro quantitative determination of anti-p53 antibodies. (roche.com)
- Anti-p53 Elecsys Anti-p53 immunoassay for the in vitro quantitative determination of anti-p53 autoantibodies in human serum and plasma. (roche.com)
- Intended as an aid in the in the diagnosis of myocardial infarction, the electrochemiluminescence (ECL) immunoassay is used for the in vitro quantitative determination of TnT-hs in human serum and plasma, the company said. (rttnews.com)
Immulite Chemiluminescent Immunoassay System1
- The IMMULITE chemiluminescent immunoassay system is designed for ease of use, reliability, high-performance assays and fully integrated operation. (bio-medicine.org)
Diagnostics3
- Developed by Roche Diagnostics, the Elecsys anti-HCV immunoassay is used to detect anti-HCV through the use of electrochemiluminescence (ECL) technology. (uspharmacist.com)
- With the completion of this acquisition, Abbott strengthened its diagnostics portfolio and established itself as a leading player in the point-of-care testing and immunoassays market. (marketsandmarkets.com)
- The increasing significance of the companion diagnostics, technological progress of immunoassay products, condition-specific biomarkers immunoassays and other emerging technologies are propelling the growth of the immunoassay market. (marketsandmarkets.com)
Diagnosis7
- The use of the automated immunoassay analyzers for cardiac ailments, endocrine diseases, and cancer has observed a rise since the last few years, owing to the accurate and speedy diagnosis capability of the product. (openpr.com)
- The immunoassay market in several countries have witnessed massive growth amid the pandemic, owing to the mass scale diagnosis of COVID-19. (marketsandmarkets.com)
- Immunoassay diagnosis has become an instrumental part of the diagnostic regime of these diseases. (marketsandmarkets.com)
- The massive adoption of the rapid tests and point-of-care testing for diseases diagnosis and drug and alcohol abuse have thrusted the growth spurt of the global immunoassay market. (marketsandmarkets.com)
- Enzyme immunoassay in the diagnosis of Lyme borreliosis. (prohealth.com)
- This innovative multiplex immunoassay is a powerful alternative to ELISAs and VNTs for veterinary diagnosis of flavivirus-related diseases. (hindawi.com)
- LAB Maastricht UMC+ in the Netherlands has a long history with myasthenia gravis, using gold standard immunoassays to enable accurate diagnosis and provide the information necessary to allow effective treatment. (tecan.com)
Solid phase immunoassays3
- Immuno modules -These modules are designed for solid phase immunoassays. (thermofisher.com)
- Immunochemical Considerations in the Analysis of Data from Non-Competitive Solid-Phase Immunoassays (J.H. Peterman). (booktopia.com.au)
- Considerations of the Interpretation of the Specificity of Monoclonal Antibodies Determined in Solid-Phase Immunoassays (F.J. Stevens). (booktopia.com.au)
Mass Spectrometric Immunoassay2
- Mass spectrometric immunoassay ( MSIA ) is a method that uses antibodies to analyze protein differences in biological samples. (wikipedia.org)
- The new Thermo Scientific Mass Spectrometric Immunoassay (MSIA) provides a robust technology designed to purify target proteins, peptides and therapeutic antibodies in a simplified way for downstream mass spectrometric analysis. (businesswire.com)
Enzyme-linked immunosorbent assays2
- Immunoassays which employ enzymes are referred to as enzyme immunoassays (EIAs), of which enzyme-linked immunosorbent assays (ELISAs) and enzyme multiplied immunoassay technique (EMIT) are the most common types. (wikipedia.org)
- The first article in this two-article series ( Automating and Miniaturizing Immunoassays: Starting the Switch to Maximum Productivity ) describes the Gyrolab™ immunoassay technology platform, focusing on its ability to save time during biopharma development and to generate higher-quality data compared with traditional enzyme-linked immunosorbent assays (ELISAs). (genengnews.com)
Reagent3
- The simplest immunoassay method measures the quantity of precipitate, which forms after the reagent antibody (precipitin) has incubated with the sample and reacted with its respective antigen to form an insoluble aggregate. (encyclopedia.com)
- Miniaturizing immunoassays can deliver tremendous savings in sample volumes, animal use, and reagent consumption. (genengnews.com)
- Immunoassay analyzers are used to identify and detect the concentration of specific substances in a sample, usually using an antibody as a reagent. (beckmancoulter.com)
Analytes5
- Immunoassay is the method of choice for measuring analytes normally present at very low concentrations that cannot be determined accurately by other less expensive tests. (encyclopedia.com)
- Analytes in biological liquids such as serum or urine are frequently measured using immunoassays for medical and research purposes. (wikipedia.org)
- Immunoassays use one or more select antibodies to detect analytes of interest. (scribd.com)
- The validation of novel biomarkers and their amalgamation into multiplex immunoassays confers the prospects of simultaneous measurement of multiple analytes in a single sample, thereby minimising cost, time and sample. (intechopen.com)
- The mass spec immunoassay (MSIA) workflow was developed to provide a simple, automated process for purification of targeted analytes for downstream detection using multiple analytical techniques, such as mass spectrometry. (tecan.com)
Analytical5
- Immunoassays are analytical techniques that are widely used to detect and quantitate the concentration of a protein, metabolite, or drug in a biological sample. (els.net)
- Immunoassay analyses reduce costs by providing real-time data, thus reducing the time spent waiting for analytical results and the amount of material that has to be excavated. (cpeo.org)
- The analytical applicability of the presented immunoassay is verified by CRP measurements in human blood serum samples. (mdpi.com)
- Analytical Comparison of the Architect Syphilis TP and Liaison Treponema Automated Chemiluminescent Immunoassays and Their Performance in a Reverse Syphilis Screening Algorithm. (harvard.edu)
- Although immunoanalytical techniques are established and advantageous over alternative screening analytical platforms, one of the challenges for immunoassays is multiplexing. (intechopen.com)
Analyzers18
- The emergence of automation in the medical sector is one of the many drivers that will fuel automated immunoassay analyzers market. (openpr.com)
- The deployment of automation in the current and forthcoming product portfolio will lead to consumers giving preference to automated immunoassay analyzers, which is likely to boost automated immunoassay analyzers industry size noticeably. (openpr.com)
- According to Global Market Insights, Inc., "Worldwide automated immunoassay analyzers market having had a revenue of USD 8 billion in 2016, will collect a revenue of USD 25 billion by 2024 with an annual growth rate of more than 15% over 2017-2024. (openpr.com)
- Speaking of which, automated immunoassay analyzers industry share from hospitals is slated to generate considerable revenue by 2024. (openpr.com)
- India is one of those regions that is likely to observe exponential growth in automated immunoassay analyzers market. (openpr.com)
- In addition, the government has also been promoting public & private partnerships to provide excellent medical service mainly across rural areas, which consequentially, will fuel Asia Pacific automated immunoassay analyzers market share considerably. (openpr.com)
- These analyzers have been gaining prominence among consumers subject to the requirement of monitoring therapeutic drugs, which is likely to augment automated immunoassay analyzers industry size substantially. (openpr.com)
- On these grounds, automated immunoassay analyzers market size from infectious diseases will collect a revenue of more than USD 7 billion by 2024. (openpr.com)
- To carry out vital activities like analysis and evaluation of test results, this product is prominently used in various diagnostic laboratories, subject to which automated immunoassay analyzers market share from diagnostic laboratories will exhibit the highest annual growth rate over the coming timeframe. (openpr.com)
- The emergence of software based technological advancements in instruments to perform multiple tests at the same time is also bound to favorably enhance the automated immunoassay analyzers industry growth. (openpr.com)
- The United States has been one of lucrative business avenues for automated immunoassay analyzers market. (openpr.com)
- Pertaining to the same, U.S. dominated North America automated immunoassay analyzers market share in 2016, by accounting for 90% of the regional contribution. (openpr.com)
- The U.S. government is also promoting the usage of automated products by implementing supportive regulatory norms, which will significantly propel U.S. automated immunoassay analyzers market share. (openpr.com)
- Chemiluminescence, basically a chemical reaction, constitutes one of the lucrative product segments of automated immunoassay analyzers industry. (openpr.com)
- The massive research, valuations and approvals of several immunoassay consumables and analyzers have alleviated the issues of the SARS-CoV2 detection. (marketsandmarkets.com)
- Beckman Coulter offers a variety of scalable immunoassay analyzers and assays, all of which can help optimize your laboratory's uptime, reliability and performance. (beckmancoulter.com)
- Extend the services of your core laboratory with our range of immunoassay analyzers and comprehensive portfolio of assays. (beckmancoulter.com)
- The new Elecsys Anti-p53 immunoassay uses the well-established electrochemiluminescence immunoassay "ECLIA" technology and is intended for use on cobas e immunoassay analyzers. (roche.com)
Assays and immunoassays2
- This high growth can be attributed to time-effectiveness and cost effectiveness provided by mass spectrometry as compared to the enzyme assays and immunoassays technology. (prnewswire.com)
- Because of the complexity and long time commitments involved in the development of such assays, particularly highly specific or efficient assays and immunoassays, many pharmaceutical companies, biotechs, and academic institutions have found that it becomes a bottleneck, holding up valuable research. (genscript.com)
MSIA1
- Discover the benefits of automation in the mass spec immunoassay (MSIA) workflow. (tecan.com)
Homogeneous immunoassay1
- Schematic representation of a homogeneous immunoassay in which, when present, the sample antigen binds to the antibody, thereby allowing access of a substrate to the active site of an enzyme labelled with the antigen. (els.net)
Multiplex immunoassay3
- Multiplex immunoassay kits, even from the same manufacturer, may not give identical results. (news-medical.net)
- Retrieved on January 28, 2020 from https://www.news-medical.net/life-sciences/Designing-a-Multiplex-Immunoassay-Factors-to-Consider.aspx. (news-medical.net)
- b) Multiplex Immunoassay Protein Standard Mix, such as ab221503 . (abcam.com)
Competitive immunoassay3
- In a competitive immunoassay. (scribd.com)
- cGMP Enzyme Immunoassay Kit is non-radioactive, competitive immunoassay for the quantitation of cyclic guanine monophosphate (cGMP) in biological fluids (serum, plasma, urine and saliva), tissue culture media samples or in samples containing very low concentrations of cyclic nucleotides. (sigmaaldrich.com)
- Non-radioactive, competitive immunoassay for the quantitation of cGMP in biological fluids (serum, plasma, urine and saliva), tissue culture media samples, or in samples containing very low concentrations of cyclic nucleotides. (sigmaaldrich.com)
Global chemiluminescence15
- The recent study by Fact.MR on global Chemiluminescence Immunoassays (CLIA) market offers a 5-year forecast between 2020 and 2025. (bccresearch.com)
- The study analyzes crucial trends that are currently determining the growth of the global chemiluminescence immunoassays (CLIA) market. (bccresearch.com)
- The study also provides dynamics that are responsible for influencing the future status of the global chemiluminescence immunoassays (CLIA) market over the forecast period. (bccresearch.com)
- A detailed assessment of the global chemiluminescence immunoassays (CLIA) market value chain analysis, business execution, and supply chain analysis across the regional markets has been covered in the report. (bccresearch.com)
- A list of prominent companies operating in the global chemiluminescence immunoassays (CLIA) market along with their product portfolio enhances the reliability of this comprehensive research study. (bccresearch.com)
- The study offers a comprehensive analysis on diverse features including production capacities, demand, product developments, revenue generation and sales in the global chemiluminescence immunoassays (CLIA) market across the globe. (bccresearch.com)
- A comprehensive estimate on the global chemiluminescence immunoassays (CLIA) market has been provided through an optimistic as well as a conservative scenario, taking into account the sales of global chemiluminescence immunoassays (CLIA) devices during the forecast period. (bccresearch.com)
- The global chemiluminescence immunoassays (CLIA) market has been analyzed for each market segment, in terms of value (US$ Mn). (bccresearch.com)
- Market estimates at global and regional levels for global chemiluminescence immunoassays (CLIA) is available in terms of "US$ Mn" for value, along with market attractiveness evaluation in the report. (bccresearch.com)
- Key sections have been elaborated in the global chemiluminescence immunoassays (CLIA) market report, which have helped to deliver projection on the regional markets. (bccresearch.com)
- These chapters include the regional macros (economic and business environment outlook), which are expected to have a momentous influence on the growth of global chemiluminescence immunoassays (CLIA) market during the forecast period. (bccresearch.com)
- Country-specific valuation on demand for global chemiluminescence immunoassays (CLIA) has been offered for each regional market, along with the market scope estimate and forecast, price index, and impact analysis of dynamics of prominence in regions and countries. (bccresearch.com)
- The report sheds light on the leading manufacturers of global chemiluminescence immunoassays (CLIA) devices along with their detailed profiles. (bccresearch.com)
- Essential and up-to-date data information related to the market performers, who are principally engaged in the production of global chemiluminescence immunoassays (CLIA) devices, has been brought with the help of a detailed dashboard view. (bccresearch.com)
- Company presence is mapped and presented through the matrix for all the prominent players thus providing the readers with actionable insights, which helps in thoughtfully presenting market status, and predicting the competition level in global chemiluminescence immunoassays (CLIA) market. (bccresearch.com)
Chemiluminescence Immunoassay1
- This report analyzes the worldwide markets for Immunoassay Systems in US$ Million by the following product segments - Enzyme Immunoassay Systems, Fluorescence Immunoassay Systems, Chemiluminescence Immunoassay Systems, Radio Immunoassay Systems, and Nephelometric Immunoassay Systems. (mdtmag.com)
Analyser2
- Drug‐of‐abuse immunoassay testing on a chemistry analyser versus a microchip array. (els.net)
- Thyrotropin (TSH), free thyroxine (fT4) and testosterone assays have been used as a probe to evaluate the performances of a new modular chemiluminescence (CL) immunoassay analyser, the Abbott Architect™ i 2000. (degruyter.com)
Biomarkers1
- BioCheck is a provider of high quality enzyme immunoassay research services and products, and a leading provider of immunoassay kits for cardiac and tumor biomarkers, infectious diseases, thyroid function, steroids, and fertility hormones. (thefreedictionary.com)
Electrochemical3
- There are a variety of detectors used in immunoassays such as enzymes, fluorophores, luminescent tags, electrochemical reactions and immunosensors. (els.net)
- Hence, we have developed a simple, cost effective, and label-free electrochemical immunoassay for the measurement of CRP in a drop of serum sample using an immunosensor strip made up of a screen printed carbon electrode (SPE) modified with anti-CRP functionalized gold nanoparticles (AuNPs). (mdpi.com)
- A new homogeneous electrochemical immunoassay strategy was developed for ultrasensitive detection of carcinoembryonic antigen (CEA) based on target-induced proximity hybridization coupled with rolling circle amplification (RCA). (rsc.org)
Protein8
- Immunoassay control set for Human Interleukin 18 protein levels. (fishersci.com)
- The instant application discloses a protein calibrator for use in the NT-proBNP immunoassays disclosed in application Ser. (freepatentsonline.com)
- 0002] This invention relates to the expression and purification of proteins, more particularly to the expression and purification of a protein calibrator used for standardization of an immunoassay, and most particularly to the expression and purification of NT-BNP used to calibrate standards in an immunoassay which measures the concentration of NT-proBNP in bodily fluids. (freepatentsonline.com)
- Sensitive immunoassay that uses an enzyme linked to an antibody or antigen as a marker for the detection of a specific protein, especially an antigen or antibody. (citizendium.org)
- By taking advantage of the Luminex ® technology, we have developed two multiplex immunoassays to monitor protein targets of depletion or retention. (sigmaaldrich.com)
- A novel immunoassay that identifies and quantifies blood levels of the PAM4 protein may detect early-stage pancreatic cancer, according to study findings presented ahead of the 2010 Gastrointestinal Cancers Symposium. (healio.com)
- Microengraving is a novel immunoassay forcharacterizing multiple protein secretions from single cells. (mdpi.com)
- Our study directly validates that protein secretion rates can be quantified by the microengraving immunoassay. (mdpi.com)
Conjugates1
- Immunoassay using antibody-enzyme conjugates. (springer.com)
Radioimmunoassay2
- Enzyme (EIA) immunoassay was developed as an alternative to radioimmunoassay (RIA). (encyclopedia.com)
- Radioimmunoassay, enzyme and non-enzyme-based immunoassays. (springer.com)
Detect4
- Immunoassays measure the formation of antibody-antigen complexes and detect them via an indicator reaction. (encyclopedia.com)
- In some cases, an immunoassay may use an antigen to detect for the presence of antibodies, which recognize that antigen, in a solution. (wikipedia.org)
- Immunoassay tests are designed to detect specific chemicals by measuring the chemicals' response to specific antibodies. (cpeo.org)
- Using the conventional monoplex immunoassay ELISA technique as a benchmark, the procedure was used to detect several grapevine and tree fruit viruses. (nih.gov)
20161
- 2016) Immunoassay. (springer.com)
Colorimetric1
- Dona, V., 1985, Homogeneous colorimetric enzyme inhibition immunoassay for cortisol in human serum with Fab anti-glucose-6-phosphate dehydrogenase as a label modulator, J. Immunol. (springer.com)
Quantification2
- On binding, they bridge a solid support, facilitating their incorporation into a format of immunoassay for particular quantification. (news-medical.net)
- The researchers used the PAM4-serum enzyme immunoassay for the detection and quantification of PAM4 in the serum of 68 patients who had surgical resection of pancreatic cancer and compared them with 19 healthy participants. (healio.com)
Specificity2
- Linearity of dilution (natural linearity, spiked linearity) is another important fundamental of immunoassay accuracy and specificity. (rndsystems.com)
- 51 monoclonal antibodies (McAb) with putative specificity for human IgA, the IgA subclasses, Am allotypes or secretory component (SC) were evaluated for immunoreactivity and specificity by nine laboratories employing immunodiffusion, agglutination, immunohistological assays, immunoblotting and direct binding and competitive inhibition enzyme immunoassays. (tudelft.nl)
Serum1
- Immunoassay determinations of the serum levels of certain proteins or carbohydrates serve as tumor markers. (cms.gov)
Antibody Detection1
- We offer two basic categories of services: 1) Immunoassay development service for drug compound discovery and 2) immunoassay development service for therapeutic antibody detection. (genscript.com)
Proteins2
- This type of immunoassay is now used in around 100 million clinical tests every year worldwide, enabling clinicians to measure a wide range of proteins, pathogens and other molecules in blood samples. (wikipedia.org)
- Quantify up to 75 proteins and peptides per well, using just 12.5 µL of sample, with FirePlex ® immunoassays. (abcam.com)
Invention relates2
- This invention relates to an element for immunoassay. (google.ca)
- The present invention relates to immunoassays, and, more particularly, to improved microscale diffusion immunoassays utilizing multivalent reactants. (google.co.uk)
Measurement1
- Taking an interdisciplinary approach that emphasizes the adaptability of immunochemical and related bioanalytical methods to a variety of matrices, Immunoassay and Other Bioanalytical Techniques describes the strength and the versatility of these methods in a wide range of environmental and biological measurement applications. (routledge.com)
Commercially available1
- Immunoassay methods are common and commercially available. (cpeo.org)
Dynamics3
- The PMR report offers an assessment of the key dynamics of the immunoassay interference blocker market which includes market drivers, industry trends, and opportunities held by the market. (bccresearch.com)
- The immunoassay interference blocker market report also studies the significant market dynamics including market drivers, trends, and restraints. (bccresearch.com)
- This report explicates on vital dynamics such as the drivers, restraints and opportunities for key market players along with key stakeholders as well as emerging players associated with the development of chemiluminescence immunoassays (CLIA). (bccresearch.com)
Interference26
- Persistence Market Research (PMR) recently published a report on the immunoassay interference blocker market that provides readers with an overall market outlook with the help of an extensive market analysis. (bccresearch.com)
- The PMR report on immunoassay interference blocker market examines the historical and current market scenario and offers a forecast for the period, 2019-2029. (bccresearch.com)
- The PMR study helps companies in this landscape gain a complete idea of the market and aid in taking key business decisions with the help of vital findings and exclusive insights into the immunoassay interference blocker market. (bccresearch.com)
- The PMR report is divided in key sections that allows the report audience to gain a detailed understanding of individual aspects of the report on the immunoassay interference blocker market. (bccresearch.com)
- How much will the immunoassay interference blocker market value in the 2029? (bccresearch.com)
- What are key trends that are likely to shape the immunoassay interference blocker market in the coming years? (bccresearch.com)
- Which type is likely to remain the sought-after immunoassay interference blocker type over the next ten years? (bccresearch.com)
- What are the key challenges that are likely to impact the immunoassay interference blocker market competitors over the years? (bccresearch.com)
- Which countries are expected to remain profitable avenues for the immunoassay interference blocker business? (bccresearch.com)
- PMR's study on immunoassay interference blocker market begins with an executive summary that sheds light on the various immunoassay interference blocker market aspects covered in the report. (bccresearch.com)
- The section offers an immunoassay interference blocker market outlook, market trends, and showcases the significance of taking up the study. (bccresearch.com)
- The next chapter in the immunoassay interference blocker market is the market overview that offers an outlook into the immunoassay interference blocker market in terms of the market scope, definition, and limitations. (bccresearch.com)
- Following this is the market background which includes a detailed understanding of the macro-economic factors impacting the immunoassay interference blocker market. (bccresearch.com)
- The next chapter offers an outlook of the global immunoassay interference blocker market analysis and forecast in terms of both volume and value. (bccresearch.com)
- The section provides an assessment of key segments in the immunoassay interference blocker market on the basis of product type, application, end user, and region. (bccresearch.com)
- Clients are able to identify promising avenues with the help of this evaluation of the immunoassay interference blocker market segments. (bccresearch.com)
- The next chapter in the PMR study on the immunoassay interference blocker market offers an evaluation of the regional landscape. (bccresearch.com)
- The segment-wise and country-level evaluation of individual regions helps the audience of the immunoassay interference blocker market study to identify potential opportunities in key regions. (bccresearch.com)
- Backed with year-on-year growth projections, global value and volume share, this section is a vital section in the report on immunoassay interference blocker market. (bccresearch.com)
- The report on immunoassay interference blocker market by PMR offers a unique competitive assessment of the key market players. (bccresearch.com)
- This sections offers an overview of the nature of the immunoassay interference blocker market with the help of the market share held by leading and emerging players. (bccresearch.com)
- The unique dashboard view of the players in the immunoassay interference blocker market allows readers to understand the key strategies implemented by individual players and their performance in the immunoassay interference blocker market featuring the focus areas of the immunoassay interference blocker market players. (bccresearch.com)
- Additionally, the competitive structure of prominent players in the immunoassay interference blocker market is also discussed in the study. (bccresearch.com)
- The PMR analysis on the immunoassay interference blocker market is based on a detailed assessment of the market including both primary and secondary research. (bccresearch.com)
- This product is the result of years of R&D aimed at creating the most powerful immunoassay interference blocker in the market. (biospace.com)
- Immunoassays used to assess thyroid function are vulnerable to different types of interference that may affect clinical decision-making. (degruyter.com)
Laboratory3
- For this reason, RIA has been largely replaced in routine clinical laboratory practice by enzyme immunoassay. (encyclopedia.com)
- Whether you run dozens or thousands of tests per day, you can rely on the same innovation and quality in our immunoassay instruments and systems that you know and trust from the rest of our total laboratory solution portfolio. (beckmancoulter.com)
- This immunoassay system supports the expansion needs of your growing laboratory. (beckmancoulter.com)