A hydroxylated form of the imino acid proline. A deficiency in ASCORBIC ACID can result in impaired hydroxyproline formation.
A polypeptide substance comprising about one third of the total protein in mammalian organisms. It is the main constituent of SKIN; CONNECTIVE TISSUE; and the organic substance of bones (BONE AND BONES) and teeth (TOOTH).
A non-essential amino acid that is synthesized from GLUTAMIC ACID. It is an essential component of COLLAGEN and is important for proper functioning of joints and tendons.
A hydroxylated derivative of the amino acid LYSINE that is present in certain collagens.
A mixed-function oxygenase that catalyzes the hydroxylation of a prolyl-glycyl containing peptide, usually in PROTOCOLLAGEN, to a hydroxyprolylglycyl-containing-peptide. The enzyme utilizes molecular OXYGEN with a concomitant oxidative decarboxylation of 2-oxoglutarate to SUCCINATE. The enzyme occurs as a tetramer of two alpha and two beta subunits. The beta subunit of procollagen-proline dioxygenase is identical to the enzyme PROTEIN DISULFIDE-ISOMERASES.
A process in which normal lung tissues are progressively replaced by FIBROBLASTS and COLLAGEN causing an irreversible loss of the ability to transfer oxygen into the bloodstream via PULMONARY ALVEOLI. Patients show progressive DYSPNEA finally resulting in death.
A complex of related glycopeptide antibiotics from Streptomyces verticillus consisting of bleomycin A2 and B2. It inhibits DNA metabolism and is used as an antineoplastic, especially for solid tumors.
A disease marked by repeated episodes of increased bone resorption followed by excessive attempts at repair, resulting in weakened, deformed bones of increased mass. The resultant architecture of the bone assumes a mosaic pattern in which the fibers take on a haphazard pattern instead of the normal parallel symmetry.
Acids derived from monosaccharides by the oxidation of the terminal (-CH2OH) group farthest removed from the carbonyl group to a (-COOH) group. (From Stedmans, 26th ed)
Experimentally induced chronic injuries to the parenchymal cells in the liver to achieve a model for LIVER CIRRHOSIS.
The process of embryo initiation in culture from vegetative, non-gametic, sporophytic, or somatic plant cells.
A metalloproteinase which degrades helical regions of native collagen to small fragments. Preferred cleavage is -Gly in the sequence -Pro-Xaa-Gly-Pro-. Six forms (or 2 classes) have been isolated from Clostridium histolyticum that are immunologically cross-reactive but possess different sequences and different specificities. Other variants have been isolated from Bacillus cereus, Empedobacter collagenolyticum, Pseudomonas marinoglutinosa, and species of Vibrio and Streptomyces. EC 3.4.24.3.
A condition due to a dietary deficiency of ascorbic acid (vitamin C), characterized by malaise, lethargy, and weakness. As the disease progresses, joints, muscles, and subcutaneous tissues may become the sites of hemorrhage. Ascorbic acid deficiency frequently develops into SCURVY in young children fed unsupplemented cow's milk exclusively during their first year. It develops also commonly in chronic alcoholism. (Cecil Textbook of Medicine, 19th ed, p1177)
Organic compounds that generally contain an amino (-NH2) and a carboxyl (-COOH) group. Twenty alpha-amino acids are the subunits which are polymerized to form proteins.
A rare amino acid found in elastin, formed by condensation of four molecules of lysine into a pyridinium ring.
A biosynthetic precursor of collagen containing additional amino acid sequences at the amino-terminal and carboxyl-terminal ends of the polypeptide chains.
Derivatives of OXALIC ACID. Included under this heading are a broad variety of acid forms, salts, esters, and amides that are derived from the ethanedioic acid structure.
An antineoplastic agent derived from BLEOMYCIN.
A fibrillar collagen consisting of three identical alpha1(III) chains that is widely distributed in many tissues containing COLLAGEN TYPE I. It is particularly abundant in BLOOD VESSELS and may play a role in tissues with elastic characteristics.
Placing of a hydroxyl group on a compound in a position where one did not exist before. (Stedman, 26th ed)
Derivatives of ACETIC ACID which contain an hydroxy group attached to the methyl carbon.
Restoration of integrity to traumatized tissue.
A solvent for oils, fats, lacquers, varnishes, rubber waxes, and resins, and a starting material in the manufacturing of organic compounds. Poisoning by inhalation, ingestion or skin absorption is possible and may be fatal. (Merck Index, 11th ed)
The most common form of fibrillar collagen. It is a major constituent of bone (BONE AND BONES) and SKIN and consists of a heterotrimer of two alpha1(I) and one alpha2(I) chains.
Polysaccharides composed of repeating galactose units. They can consist of branched or unbranched chains in any linkages.
A specialized CONNECTIVE TISSUE that is the main constituent of the SKELETON. The principle cellular component of bone is comprised of OSTEOBLASTS; OSTEOCYTES; and OSTEOCLASTS, while FIBRILLAR COLLAGENS and hydroxyapatite crystals form the BONE MATRIX.
Any pathological condition where fibrous connective tissue invades any organ, usually as a consequence of inflammation or other injury.
Bone loss due to osteoclastic activity.
Either of the pair of organs occupying the cavity of the thorax that effect the aeration of the blood.
An enzyme that catalyzes the conversion of an orthophosphoric monoester and water to an alcohol and orthophosphate. EC 3.1.3.1.
Surgical union or shunt between ducts, tubes or vessels. It may be end-to-end, end-to-side, side-to-end, or side-to-side.
Disorders affecting amino acid metabolism. The majority of these disorders are inherited and present in the neonatal period with metabolic disturbances (e.g., ACIDOSIS) and neurologic manifestations. They are present at birth, although they may not become symptomatic until later in life.
Heteropolysaccharides which contain an N-acetylated hexosamine in a characteristic repeating disaccharide unit. The repeating structure of each disaccharide involves alternate 1,4- and 1,3-linkages consisting of either N-acetylglucosamine or N-acetylgalactosamine.
Liver disease in which the normal microcirculation, the gross vascular anatomy, and the hepatic architecture have been variably destroyed and altered with fibrous septa surrounding regenerated or regenerating parenchymal nodules.
A non-metal element that has the atomic symbol P, atomic number 15, and atomic weight 31. It is an essential element that takes part in a broad variety of biochemical reactions.
Naturally occurring or experimentally induced animal diseases with pathological processes sufficiently similar to those of human diseases. They are used as study models for human diseases.
The first enzyme of the proline degradative pathway. It catalyzes the oxidation of proline to pyrroline-5-carboxylic acid in the presence of oxygen and water. The action is not reversible. The specific activity of proline oxidase increases with age. EC 1.5.3.-.
A plant genus of the family Musaceae, order Zingiberales, subclass Zingiberidae, class Liliopsida.
A strain of albino rat developed at the Wistar Institute that has spread widely at other institutions. This has markedly diluted the original strain.
Excretion of an excessive amount of OXALATES in the urine.

Variations in 35SO4 incorporation into glycosaminoglycans along canine coronary arteries. A possible index of artery wall stress. (1/1144)

Focal areas of accentuated wall stress along the course of canine coronary arteries may be revealed by the level of 35SO4 incorporation into glycosaminoglycans (GAG). In the anterior descending artery, 35SO4 incorporation in higher in the proximal than in the distal region and may be extraordinarily high as the vessel enters a proximally located muscle bridge and at the takeoff region of multidirectional branches. In the circumflex artery, the incorporation also is higher in the proximal than in the distal region and is high at the genu where the posterior descending artery forms. There are differences in uptake of 35SO4 in vessels even when the arteries arise from the same vascular bed.this was shown by the higher incorporation in the left coronary artery than in the right coronary artery. A general anatomical agreement exists between these sites of high 35SO4 incorporation and previously described locations of interval elastic disruption ans proliferation of intimal connective tissue in the dog.  (+info)

Suppression of experimental abdominal aortic aneurysms by systemic treatment with a hydroxamate-based matrix metalloproteinase inhibitor (RS 132908). (2/1144)

BACKGROUND: Abdominal aortic aneurysms (AAAs) are associated with chronic inflammation, disruption of medial elastin, and increased local production of elastolytic matrix metalloproteinases (MMPs). The purpose of this study was to investigate how treatment with a hydroxamate-based MMP antagonist (RS 132908) might affect the development of experimental AAAs. METHODS: Male Wistar rats underwent intraluminal perfusion of the abdominal aorta with 50 units of porcine pancreatic elastase followed by treatment for 14 days with RS 132908 (100 mg/kg/day subcutaneously; n = 8) or with vehicle alone (n = 6). The external aortic diameter (AD) was measured in millimeters before elastase perfusion and at death, with AAA defined as an increase in AD (DeltaAD) of at least 100%. Aortic wall elastin and collagen concentrations were measured with assays for desmosine and hydroxyproline, and fixed aortic tissues were examined by light microscopy. RESULTS: AAAs developed in all vehicle-treated rats, with a mean AD (+/- SE) that increased from 1.60 +/- 0.03 mm before perfusion to 5.98 +/- 1.02 mm on day 14 (DeltaAD = 276.4 +/- 67.7%). AAAs developed in only five of eight animals (62.5%) after MMP inhibition, with a mean AD that increased from 1.56 +/- 0.05 mm to 3.59 +/- 0.34 mm (DeltaAD = 128.1 +/- 18.7%; P <.05, vs vehicle). The overall inhibition of aortic dilatation attributable to RS 132908 was 53.6 +/- 6.8%. Aortic wall desmosine fell by 85.4% in the vehicle-treated rats (1210.6 +/- 87.8 pmol/sample to 176.7 +/- 33.4 pmol/sample; P <.05) but only by 65.6% in the animals treated with RS 312908 (416.2 +/- 120.5 pmol/sample). In contrast, hydroxyproline was not significantly affected by either elastase perfusion or drug treatment. Microscopic examination revealed the preservation of pericellular elastin and a greater degree of fibrocollagenous wall thickening after MMP inhibition, with no detectable difference in the extent of inflammation. CONCLUSIONS: Systemic MMP inhibition suppresses aneurysmal dilatation in the elastase-induced rodent model of AAA. Consistent with its direct inhibitory effect on various MMPs, RS 132908 promotes the preservation of aortic elastin and appears to enhance a profibrotic response within the aortic wall. Hydroxamate-based MMP antagonists may therefore be useful in the development of pharmacologic approaches to the suppression of AAAs.  (+info)

Altered connective tissue in children with congenital dislocation of the hip. (3/1144)

The umbilical cord was employed as a source of collagen in 10 children with congenital dislocation of hip. The amount of collagen and its solubility were measured in slices of the cords and in the umbilical veins and compared with the values in normal subjects. Both the amount of collagen and its solubility were decreased in children with congenital dislocation of the hip.  (+info)

Effects of pirfenidone on procollagen gene expression at the transcriptional level in bleomycin hamster model of lung fibrosis. (4/1144)

A time course study was carried out to elucidate the mechanisms for antifibrotic effect of pirfenidone (PD). Hamsters were intratracheally (i.t.) instilled with saline (SA) or bleomycin (BL) (7.5 units/kg/5 ml). The animals were fed a diet containing 0.5% PD or the same control diet (CD) without the drug 2 days before and throughout the study. The animals were sacrificed at various times after instillation. The lung hydroxyproline level in BL + CD groups was gradually increased and peaked at 21 days to 181% of the SA + CD control. The BL + PD-treated groups showed a gradual decrease in their lung collagen content, showing a maximum reduction of 40% at day 21. The lung malondialdehyde levels of the BL + CD groups were increased by several-fold of the corresponding SA + CD groups at various times. The lung prolyl hydroxylase (PH) activities in the BL + CD groups were also increased by several-fold of the corresponding SA + CD groups at these time points. The hamsters in the BL + PD showed a gradual decrease in the lung malondialdehyde levels from 10 to 21days compared with their corresponding BL + CD groups. Treatment with PD also reduced the lung PH activities in the BL + PD groups compared with the corresponding BL + CD groups. However, PD failed to manifest any direct inhibitory effect on PH activity in vitro. BL treatment increased the lung procollagen I and III gene expressions in the BL + CD groups by several-fold at varying times compared with the corresponding SA + CD, and treatment with PD in the BL + PD groups significantly down-regulated the BL-induced overexpression of these genes. Studies evaluating the regulation of these genes at the transcriptional level revealed PD significantly reduced the transcription of PC I at 14 days. Our results indicate that the antifibrotic effect of PD was partly due to suppression of the BL-induced inflammatory events and partly due to down-regulation of BL-induced overexpression of lung procollagen I and III genes.  (+info)

A new approach to assessing collagen turnover by using a micro-assay. A highly efficient and rapid turnover of collagen in rat periodontal tissues. (5/1144)

Measurement of [3H]proline incorporation into newly synthesized and mature collagen in connective tissues was used to compare rates and efficiency of collagen turnover. The approach minimizes label-recycling problems. By using a micro-assay to determine hydroxyproline specific radioactivities, a highly efficient and rapid collagen turnover in rat periodontal tissues was demonstrated.  (+info)

T cell independence of bleomycin-induced pulmonary fibrosis. (6/1144)

The role of T cells and cytokines in bleomycin (BLM)-induced fibrosis was evaluated in susceptible and resistant strains of normal and SCID mice. Histology and hydroxyproline analysis showed that BLM induced pulmonary fibrosis in C57BL/6 and (C57BL/6 x BALB/c)F1 mice, whereas BALB/c mice were resistant to the disease. To test whether lymphocytes were required for the induction of BLM-induced pulmonary fibrosis, SCID mice were injected intratracheally with BLM and evaluated for the development of pulmonary inflammation and fibrosis. Similar morphological changes and increases in hydroxyproline were observed in both C57BL/6 SCID and (C57BL/6 x CB.17)F1 SCID animals compared to those seen in wild-type C57BL/6 and (C57BL/6 x BALB/c)F1 mice. In contrast, CB.17 SCID mice, which are genetically similar to BALB/c mice, were resistant to disease induction. Analysis of the cellular infiltrate in BLM-treated C57Bl/6 SCID mice confirmed a lack of T cells in the lungs of SCID mice and demonstrated a pronounced accumulation of eosinophils in areas of developing pulmonary fibrosis. NK cells were significantly elevated in untreated SCID mice and did not increase further after BLM treatment. Analysis of selected cytokines 1 day after initiation of BLM-induced pulmonary fibrosis indicated that the levels of TNF-alpha and IFN-gamma appeared to segregate with fibrosis in both the SCID and wild-type mice. The data demonstrate that T cells are not required for the induction of fibrosis by BLM and suggest that responses by non-lymphoid cells may be sufficient for the induction of fibrosis.  (+info)

The action of the benzopyrones on an experimental model of lymphoedema: a contribution to their mode of action. (7/1144)

A number of preparations containing benzopyrones are used clinically as a therapy for lymphoedema; however, their exact mode of action is not well known. This work presents evidence which indicates that, as in the treatment of thermally induced oedemas, the benzopyrones work by enhancing the lysis of the accumulated proteins. This is evidenced by reduced levels of total protein in the extracellular compartment of the skin, while peptides and amino acids were increased in the serum at 6 and 12 h respectively after the drug's administration. Failure to observe very marked increases in peptides and amino acids at other times in the serum and skin was attributed to the rapid incorporation of these into the large number of maturing phagocytes which enter the lymphoedematous tissues. Likewise, protease activity levels were not elevated as expected. This possibly was the consequence of a number of factors including serum deactivation, inhibition of release and membrane stabilization.  (+info)

Histology and tissue chemistry of tidemark separation in hamsters. (8/1144)

Adult articular cartilage is divided by the tidemark into a deep calcified layer and a more superficial uncalcified layer. Histologic examination of articular cartilage from the knee joint of golden Syrian hamsters 123 days of age or older revealed defects at the tidemark in the tibia. Defects ranged from small separations of the calcified and uncalcified layers along the tidemark to progressively larger defects apparently formed by dissolution. These larger defects appeared as cavities in the noncalcified cartilage, had smooth rather than rough edges, frequently contained coalesced debris, and often resulted in a bulge in the articular surface. Occasionally, these large defects broke through the articular surface. Defects were not observed in tibial cartilage of younger (<90 days old) hamsters or in femoral cartilage from hamsters of any age. Exercise neither protected against nor increased the severity of the defects. Collagen cross-linking by pyridinoline was examined as a function of age and increased from 1,090 to 3,062 micromoles of pyridinoline/mole of hydroxyproline over the period of 1-9 months of age but was not correlated with defect formation. With increasing age, these focal tidemark defects could lead to osteoarthrosis-like cartilage lesions.  (+info)

TY - JOUR. T1 - Serial spot hydroxyproline/creatinine ratios in metastatic prostatic cancer. AU - Hopkins, S. C.. AU - Nissenkorn, I.. AU - Palmieri, G. M.A.. AU - Ikard, M.. AU - Moinuddin, M.. AU - Soloway, M. S.. PY - 1983/1/1. Y1 - 1983/1/1. N2 - Analysis of urinary hydroxyproline levels offers a marker to monitor osseous involvement in patients with metastatic malignancies. Such a marker is needed in patients with prostatic cancer when bone metastases predominate. Thirty-two men with stage D2 prostatic cancer were monitored by bone scan, acid and alkaline phosphatase values, and urinary hydroxyproline, beginning from 4 to 36 months after initiation of hormonal manipulation and/or systemic chemotherapy. In patients with disease progression determined by bone scan serial urinary hydroxyproline values progressively increased and were significantly elevated compared to urinary values obtained from patients with a stable or improving scan (p ,0.001). Simultaneous alkaline phosphatase ...
Trans-3-Hydroxy-L-Proline CAS: 4298-08-2 Amino Acid Pharmaceutical Grade Intermediates picture from Wuhan HengHeDa Pharm Co., Ltd. view photo of Trans-3-Hydroxy-L-Proline, 4298-08-2, Amino Acids.Contact China Suppliers for More Products and Price.
Diseases such as liver fibrosis and intestinal inflammation are characterized by accumulated components of the extracellular matrix (ECM). Given that fibrillar collagen structures were shown to serve as storage site for inactive proforms of matrixmetalloproteinases (MMPs), modulating this MMP-collagen interaction might offer a rational interventional (therapeutic) approach to enhance degradation of accumulated ECM. The synthetic triple helical collagen analogue (Gly-Pro-Hyp) 10 - (GPO) 10 - was shown to trigger release and enzymatic activation of collagen sequestered proMMP-2. In the presented study, we, for the first time, investigated how MMP-(GPO) 10 interaction impacts cellular responses in vitro . We found that recombinant proMMP-2 induced proliferation of hepatic stellate cells (HSC), which was enhanced after addition of (GPO) 10 reaching comparable levels following incubation with fully activated MMP-2. In addition, (GPO) 10 induced HSC migration similar to the platelet-derived growth
en] Chloro-4-phenyl thiomethylene bisphosphonate (tiludronate) is a new drug which can be used as an inhibitor of bone resorption. As it remains in bone for a long time, and as mineralisation defects have only been seen at doses much higher than those required to decrease osteoclastic activity, it could be given at high doses over a short period of time. Eighteen patients with Pagets disease of bone were randomly allocated to three therapeutic groups receiving respectively 600, 800, and 1200 mg/day tiludronate for five days. Serum alkaline phosphatase activity and the urinary hydroxyproline/creatinine ratio were quickly and drastically reduced in all three groups. A significant reduction of serum alkaline phosphatases and the hydroxyproline/creatinine ratio was still present six months after the five day therapeutic course, reflecting a sustained activity of tiludronate even after stopping treatment. Dose dependent short and long term reductions of bone turnover rate were observed. Biochemical ...
We report data from three patients with severe Pagets disease of bone who were treated with mithramycin.. Mithramycin infusion resulted in a fall in plasma calcium, phosphate, alkaline phosphatase, and urinary hydroxyproline excretion. There was an improvement in calcium and phosphorus balance in two of the three subjects studied. A pronounced or complete relief of bone pain occurred in all three.. We suggest that mithramycin exerts its beneficial effect in Pagets disease of bone by stimulating parathyroid hormone release. The parathyroid hormone released has a predominantly anabolic action on bone since its catabolic action is blocked by mithramycin, which inhibits bone resorption.. ...
Saline extracts of potato tubers contain a potent hemagglutininin that is not inhibited by monosaccharides. Carbohydrate inhibition studies have shown that the lectin has an extended binding site that can accommodate up to four carbohydrate units. The lectin binds oligosaccharides containing β(1,4)-linked GlcNAc. The tetrasaccharide is approximately 20 times more potent an inhibitor than the disaccharide 1 . STA also reacts with poly N-acetyllactosamine structures from N-linked oligosaccharides, albeit rather weakly 2 . Nevertheless, it is useful for histochemical staining of these structures 3 . Many lectins do not react with sulfated sugars, however, STA does bind to chitin sulfates and keratan sulfates 4 . Purified STA is one of the most highly glycosylated lectins, composed of approximately 50% carbohydrate by weight. The heavily-glycosylated portion is a hydroxyproline-containing sequence homologous to the cell wall extensions, while the carbohydrate-binding region is rich in cysteine and ...
Young (3 mo old) and old (18-24 mo old) wild-type (WT) and SPARC-null mice were studied. LV collagen content was measured histologically by collagen volume fraction, collagen composition was measured by hydroxyproline assay as soluble collagen (1 M NaCl extractable) versus insoluble collagen (mature cross-linked), and collagen morphological structure was examined by scanning electron microscopy ...
Askalite Formula: Teff-powered, premium science backed treats for skin of color. This is a peptide sourced from natural proteins and mimics the endogenous collagen forming amino acid version hydroxyproline. The collagen in the dermis is triple-helical structure of glycine, proline and hydroxyproline.
Description: The chemical reactions and pathways involving 4-hydroxyproline, C5H9NO3, a derivative of the amino acid proline. The presence of hydroxyproline is essential to produce stable triple helical tropocollagen, hence the problems caused by ascorbate deficiency in scurvy. This unusual amino acid is also present in considerable amounts in the major glycoprotein of primary plant cell walls.. ...
Yup, Ive been expressing bewilderment for months now as to why STURPs Ray Rogers (left) thought there was hydroxyproline in human blood. He cited the presence of hydroxyproline as crucial evidence against the Shroud blood ever having been heated, using that to dismiss any idea that the body image was a thermal imprint (scorch) -…
MIT and Harvard University chemists have discovered the structure of an unusual bacterial enzyme that can break down an amino acid found in collagen, which is the most abundant protein in the human body. The enzyme, known as hydroxy-L-proline dehydratase (HypD), has been found in a few hundred species of bacteria that live in the human gut, including Clostridioides difficile. The enzyme performs a novel chemical reaction that dismantles hydroxy-L-proline, the molecule that gives collagen its tough, triple-helix structure. Now that researchers know the structure of the enzyme, they can try to develop drugs that inhibit it. Such a drug could be useful in treating C. difficile infections, which are resistant to many existing antibiotics. This is very exciting because this enzyme doesnt exist in humans, so it could be a potential target, says Catherine Drennan, PhD, an MIT Professor of Chemistry and Biology and a Howard Hughes Medical Institute Investigator. If you could potentially inhibit that ...
The most important problem relating to safety and scientific feasibility of regular intake of MCPs is elevated from the nicely founded fact that the induction of collagen synthesis, generally assessed through the greater hydroxyproline concentrations, is usually connected to oxidative worry [28-thirty]. Also, MCPs of various origin are already proven to activate innate immune reaction of macrophages and neutrophils via Toll-like receptor 4, which leads to NADPH-oxidase (NOX4) activation and reactive oxygen species overproduction [31, 32 ...
Maggadani, B. P., ., H., & Lubnan, M. (2018). VALIDATION METHODS FOR THE ANALYSIS OF HYDROXYPROLINE FROM COLLAGEN UNDENATURED TYPE II COLLAGEN USING HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY FLUORESCENCE. International Journal of Applied Pharmaceutics, 10(1), 403-406. https://doi.org/10.22159/ijap.2018. ...
[65 Pages Report] Check for Discount on TRANS METHYL O-BENZYL-L-4-HYDROXYPROLINE Global Market and Forecast Research report by ChemReport. DescriptionWe provide independent and unbiased information on manufacturers, prices, production...
As a clarification of the above, molecular oxygen, as I understand it, is necessary for the post-transcriptional hydroxylation of proline to hydroxyproline - ie the oxygen in the hydroxyproline does not come from water, but is added from an O2 molecule by an oxygenase enzyme.. The hydroxyprolines are critical for collagen strength. Thus in the complete absence of O2, animals bodies simply would not be able to hold together beyond a certain size (hence my first question about whether or not the small size gets around the problem).. The level of O2 needed is actually much lower than current atmospheric O2 in aerobic environments, and may be low enough that it could be found even in environments that would be considered to be hypoxic. But the post seemed to suggest that these animals lived in a completely anoxic environment - there is no O2 at all? If which case, how do these animals synthesize collagen?. ...
In plants and animals, small peptide ligands that signal in cell-cell communication have been suggested to be a crucial component of development. A bioassay of single-cell transdifferentation demonstrates that a dodecapeptide with two hydroxyproline residues is the functional product of genes from the CLE family, which includes CLAVATA3 in Arabidopsis. The dodecapeptide suppresses xylem cell development at a concentration of 10-11 M and promotes cell division. An application, corresponding to all 26 Arabidopsis CLE protein family members, of synthetic dodecapeptides reveals two counteracting signaling pathways involved in stem cell fate. ...
Rabbit polyclonal Hydroxyproline antibody validated for WB, IHC. Referenced in 3 publications and 3 independent reviews. Immunogen corresponding to synthetic…
The network for 4 hydroxyproline metabolic process in your query organism is displayed on the left, if relationships are supported by the integrated data. Moving any of the genes in that network will simultaneously update the homologs in the networks displayed to the right (if they exist). Additionally, hovering over any nodes will highlight the identified functionally similar homologs in the other networks. Last, the bar above the networks allows you to remove/add additional organisms. Simily drag and drop the organism names in the desired order. ...
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If you wish to reuse any or all of this article please use the link below which will take you to the Copyright Clearance Centers RightsLink service. You will be able to get a quick price and instant permission to reuse the content in many different ways.. ...
TABLE-US-00001 NAME OF RAW MATERIAL WEIGHT % (MANUFACTURER) INCI 1 2 3 4 5 6 7 8 9 10 Pseudoceramides Pseudoceramide 176 N-(1-dodecanoyl)-4- 0.1 0.2 0.1 hydroxy-L-proline 1- hexadecyl ester Pseudoceramide 391 N-(1-hexadecanoyl)-4- 0.2 0.1 0.1 0.1 0.2 0.5 hydroxy-L-proline 1- hexadecyl ester Ceramide PC104 (Pacific hydroxypropyl- 0.1 Corporation) bispalmitamide MEA Ceramide SL (Sino Lion) hydroxyethyl-palmityl- 0.1 oxyhydroxypropyl- palmitamide Aqua-Ceramide (Kao) cetyloxypropyl-glyceryl- 0.1 0.1 methoxypropyl- myristamide Ceramides Ceramide 2 (Sederma) ceramide 2 0.1 Ceramide III (Cosmoferm) ceramide 3 0.1 Bisabolol (Alpha-)Bisabolol, natural bisabolol 0.2 0.3 0.2 0.5 0.1 (Symrise) Dragosantol (Symrise) bisabolol 0.3 0.4 0.2 0.3 0.3 Fatty acids Linoleic Acid linoleic acid 0.1 0.2 Palmitic Acid palmitic acid 0.1 0.1 0.2 Stearic Acid stearic acid 0.1 Sterols Cholesterol (Croda) cholesterol 0.1 0.5 Tamasterol (Tama Biochemicals) phytosterol 0.2 Other ingredients Abil 350 (Degussa- dimethicone 0.5 ...
Polyribosomes containing nascent [3H]proline-labeled collagen chains were isolated from chick embryo fibroblasts in culture. These nascent chains were nearly completely hydroxylated, as indicated by the presence of [3H]hydroxyproline and high hydroxyproline/proline ratios. The polyribosomes were suspended in D2O at 15 degrees and the infrared spectrum was determined using a reference cell containing collagen-depleted polyribosomes in D2O, matched to equal RNA content. The amide I and amide II bands were observed. When the polyribosomes were heated in D2O at 44 degrees in the infrared cells, the N--D amide II absorbance at 1480 cm-1 increased markedly, indicating that H leads to D exchange had occurred. Collagen-depleted polyribosomes showed no such changes in absorbance at 1450-1480 cm-1 upon heating. Polyribosomes recovered from the infrared cells after treatment at 44 degrees and cooling still contained collagen, as indicated by their [3H]hydroxyproline content. These data indicate that ...
Hello Kapil,. Thanks for your interesting questions.. Sorry for the delayed replay. I was swamped in my work for a while, needless to explain the pressure.. Answer to your 1st question: As we all know, speed vac or centrifugal evaporator works by reducing the pressure under vacuum so does the boiling point of a given solvent. The centrifugal force generated during this process will evaporate the solvent from top, thus it prevent loss or solute by solvent bumping (in other words, by violent boiling). During centrifugal evaporation of HCl from our sample, we all assume that there will be a small loss of hydroxy proline (HYP) but this will be a similar error if we treat all our samples in the same way. It is known that the loss of HYP on drying is ≤ 0.1% at 110 C and I am sure centrifugal evaporator with no heat (assuming we are not using heating option in the instrument) will not reach this temp.. In summary, you have to dry the HCl in the absence of heat in centrifugal evaporator. If you are ...
The intrinsic capacity of tendons to heal in response to injury has recently been demonstrated by many investigators. Electrical stimulation is often assumed to augment regeneration of various tissues. Using newly developed methods of whole-tendon culture, the authors examined the effect of direct-current electricity on healing in vitro. Deep flexor tendons of rabbits were excised, transected, repaired, and grown in an acellular culture medium for seven, 14, 21, or 42 days. Tendons through which a continuous 7-microAmp current was passed at the repair site were compared with nonstimulated controls. The incorporation of (14C)proline and its conversion to (14C)hydroxyproline was measured at seven days. The mean (14C)proline and (14C)hydroxyproline activities were 91% and 255% greater, respectively, in the stimulated group. The activity was also higher in the stimulated group, by 42 days. Histologic sections showed that intrinsic tenoblastic repair may be enhanced with electrical stimulation in ...
The collagen triple helix is stabilized by interchain hydrogen bonds. The sequence of the protein in the helical region consists of multiple repeats of the form -Gly-X-Y-, where X is often proline and Y is often a modified proline called 4-hydroxyproline. The glycine residues are located along the central axis of the triple helix, where tight packing of the protein strands can accommodate no other residue. For each -Gly-X-Y- triplet, one hydrogen bond forms between the amide hydrogen atom of glycine in one chain and the carbonyl oxygen atom of residue X in an adjacent chain. Hydrogen bonds involving the hydroxyl group of hydroxyproline may also stabilize the collagen triple helix. Unlike the more common α helix, the collagen helix has no intrachain hydrogen bonds ...
Background. Bone mineral density (BMD) is used to follow gain or loss of bone mass but cannot detect changes within a short period of time. Biochemical markers of bone turnover may be of value for prediction of individual bone loss.. Methods. We studied the relation between common inexpensive markers of bone turnover (serum alkaline phosphatase (ALP), osteocalcin (OC), urinary hydroxyproline (OHPr), and calcium (Ca)), BMD, age, and menopause in a combined cross-sectional and longitudinal design comprising 429 pre- and postmenopausal randomly selected women aged 21-79 years (mean 50 years). A follow-up was initiated after 5 years (including 192 of these women), which focused on changes in bone mass and the ability of these four common markers of bone turnover (sampled at baseline) to predict future bone loss.. Results. A marked increase was observed for all markers at the beginning of menopause. During the postmenopausal period ALP and Ca decreased to near premenopausal levels, while OC and OHPr ...
Proline has been grafted onto silica supports in a single step by reacting trans-4-hydroxy-l-proline with chloropropyl tethers, without the use of protecting groups for the proline amine and carboxylic acid functional groups. The resulting catalysts have been characterised to show that grafting is through re Catalysis on Chiral Surfaces: From Fundamental Aspects to Application
Stat4(-/-) mice were protected against bleomycin-induced dermal fibrosis, with a reduction in dermal thickening (mean ± SEM 65 ± 3% decrease; P = 0.03), hydroxyproline content (68 ± 5% decrease; P = 0.02), and myofibroblast counts (71 ± 6% decrease; P = 0.005). Moreover, the number of infiltrating leukocytes, especially T cells, was significantly decreased in the lesional skin of stat4(-/-) mice (mean ± SEM 63 ± 5% reduction in T cell count; P = 0.02). Stat4(-/-) mice also displayed decreased levels of inflammatory cytokines such as tumor necrosis factor α, interleukin-6 (IL-6), IL-2, and interferon-γ in lesional skin. Consistent with a primary role of STAT-4 in inflammation, STAT-4 deficiency did not ameliorate fibrosis in TSK-1 mice ...
Anti-fibrosis treatment with prizaquantel reduced areas of liver Sirius Red staining and liver hydroxyproline contents of mice 8 weeks post infection with Schis
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Soluble Collagen refers to the big, natural collagen molecules mostly extracted from fish or bovine skin. Spotting collagen on the ingredient...
Prolyl 4-hydroxylase (EC 1.14.11.2) catalyzes the formation of4-hydroxyproline in collagens by the hydroxylation of proline residues inX-Pro-Gly sequences. The reaction requires Fe2+, 2-oxoglutarate, O2, andascorbate and involves an oxidative decarboxylation of 2-oxoglutarate.Ascorbate is not consumed during most catalytic cycles, but the enzymealso catalyzes decarboxylation of 2-oxoglutarate without subsequenthydroxylation, and ascorbate is required as a specific alternative oxygenacceptor in such uncoupled reaction cycles. A number of compounds inhibitprolyl 4-hydroxylase competitively with respect to some of itscosubstrates or the peptide substrate, and recently many suicideinactivators have also been described. Such inhibitors and inactivatorsare of considerable interest, because the prolyl 4-hydroxylase reactionwould seem a particularly suitable target for chemical regulation of theexcessive collagen formation found in patients with various fibroticdiseases. The active prolyl 4-hydroxylase ...
Gelatin is a form of hydrolyzed collagen which brings a ready supply of four essential amino acids: glycine, proline, hydroxyproline, and glutamic acid. Nails and skin benefit from these amino acids.
Bleomycin-induced lung fibrosis in mice reproduces some key features of pulmonary fibrosis in humans including alveolar inflammation, myofibroblast proliferation, and collagen deposition. Glucocorticoids have been used as first-line therapy for the treatment of lung fibrosis, although their clinical efficacy is equivocal. We examined the effect of the glucocorticoid, methylprednisolone (MP), and the estrogen metabolite, 2-methoxyestradiol (2MEO) on bleomycin-induced bronchoalveolar inflammation, fibrosis, and changes in lung function. The characterization of the time-course of the bleomycin-induced fibrosis indicated that lung dry mass and hydroxyproline content showed less variance than histopathological assessment of fibrosis. The bleomycin-induced increases in bronchoalveolar lavage (BAL) fluid cell number and protein levels were not significantly influenced by treatment with either MP (1 mg.(kg body mass)(-1).day(-1), i.p.) or 2MEO (50 mg.(kg body mass)(-1).day(-1), i.p.). Lung fibrosis, ...
INTRODUCTION: Preterm cervical shortening and cervical insufficiency may be caused by a constitutional weakness of the cervix. The aim of this study was to assess the cervical collagen concentration in non-pregnant women with a history of cervical insufficiency or of a short cervix in the second trimester of pregnancy.. MATERIAL AND METHODS: In this case-control study we included non-pregnant women one year or more after pregnancy: 55 controls with a history of normal delivery; 27 women with a history of cervical insufficiency; and 10 women with a history of a short cervix (,5th percentile) and 10 women with a history of a long cervix (,95th percentile) at gestational weeks 18-20. We obtained biopsies (3 × 3-4 mm) from the ectocervix and determined the collagen concentration by measuring the hydroxyproline concentration.. RESULTS: Women with cervical insufficiency had lower collagen concentrations (63.5 ± 5.1%; mean ± SD) compared with controls (68.2 ± 5.4%; p = 0.0004); area under the ROC ...
Major changes in bone mineral density (BMD) and body composition occur during puberty. In the present longitudinal study, we evaluated BMD and calculated volumetric BMD [bone mineral apparent density (BMAD)], bone metabolism, and body composition of children (32 girls and 2 boys) with central precocious and early puberty before and during treatment with GnRH agonist (GnRH). Patients were studied at baseline and during treatment for 6 months (n = 34), 1 yr (n = 33), and 2 yr (n = 16). Lumbar spine and total body BMD and body composition were measured with dual-energy x-ray absorptiometry. The variables were compared with age- and sex-matched reference values of the same population and expressed as SD score (SDS). Bone age was assessed. Serum calcium, phosphate, alkaline phosphatase, osteocalcin, the carboxyterminal propeptide of type I collagen (PICP), cross-linked telopeptide of collagen I (ICTP), 1,25 dihydroxyvitamin D and urinary hydroxyproline/creatinine, and calcium/ creatinine ratios were ...
Background: ACE inhibitors have shown beneficial results in several studies after myocardial infarction (MI). However, these studies have shown conflicting results about the ideal starting time of the ACE inhibitors administration after MI and the importance of infarct size.Objectives: This study was designed to assess the long-term effects of lisinopril on mortality, cardiac function, and ventricular fibrosis after MI, in rats.Methods: Lisinopril (20 mg/kg/day) was given on day 1 or 21 days after coronary occlusion in small or large infarctions.Results: the mortality rate was reduced by 39% in early treatment and 30% in delayed treatment in comparison to the untreated rats. Early treatment reduced cardiac dysfunction in small MIs; however, delayed treatment did not. No statistical difference was observed among the groups for large MIs. No statistical difference was observed among the groups with large or small MIs on myocardial hydroxyproline concentration.Conclusions: Both early and delayed ...
The specific role of Toll-like receptor 4 (TLR4) in bleomycin-induced lung fibrosis of mice, a model of human idiopathic pulmonary fibrosis, has not been characterized. We injected bleomycin intratracheally into TLR4 knockout (TLR4-/-) and wild-type (WT) mice. Twenty-one days after injection, mice were sacrificed and their lungs were harvested for pathological, hydroxyproline, mRNA expression, and collagen I analyses. Body weight changes and mortality were observed.
Members of this family belong to the collagen superfamily [ (PUBMED:8240831) ]. Collagens are generally extracellular structural proteins involved in formation of connective tissue structure. The sequence is predominantly repeats of the G-X-Y and the polypeptide chains form a triple helix. The first position of the repeat is glycine, the second and third positions can be any residue but are frequently proline and hydroxyproline. Collagens are post-translationally modified by proline hydroxylase to form the hydroxyproline residues. Defective hydroxylation is the cause of scurvy. Some members of the collagen superfamily are not involved in connective tissue structure but share the same triple helical structure.. ...
Results TRB3 expression was increased in fibroblasts of patients with SSc and in murine models of SSc in a transforming growth factor-β (TGF-β)/Smad-dependent manner. Overexpression of TRB3 stimulated canonical TGF-β signalling and induced an activated phenotype in resting fibroblasts. In contrast, knockdown of TRB3 reduced the profibrotic effects of TGF-β and decreased the collagen synthesis. Moreover, siRNA-mediated knockdown of TRB3 exerted potent antifibrotic effects and ameliorated bleomycin as well as constitutively active TGF-β receptor I-induced fibrosis with reduced dermal thickening, decreased hydroxyproline content and impaired myofibroblast differentiation. ...
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Results Decreased expression of PARP1 was detected by immunohistochemistry in skin sections of SSc patients, particularly in fibroblasts. Inhibition of PARylation by 3AB augmented the stimulatory effects of TGFβ on fibroblasts in vitro. PARP1 inhibition increased Smad dependent transcription in reporter assays and promoted the transcription of TGFβ/Smad target genes. Treatment with 3AB also stimulated the collagen release and fostered the expression of contractile proteins with increased expression of α-smooth muscle actin (α-SMA) and enhanced formation of stress fiber formation compared to fibroblasts stimulated with TGFβ alone. Inhibition of PARylation also exacerbated experimental fibrosis in vivo. Treatment with 3AB induced a more severe fibrotic response to bleomycin with increased dermal thickening by up to 103% (p,0.0001), hydroxyproline contents and myofibroblast counts compared to control mice (p,0.0001 and p=0.0059). Inhibition of PARylation also strongly exacerbated fibrosis in ...
Keeping your skins collagen levels in check is so important and we cant say it enough! Read on to get some beauty tips and know more about how Payot Roselift Collagène range can help boost collagen naturally.
This gene encodes a component of prolyl 4-hydroxylase, a key enzyme in collagen synthesis composed of two identical alpha subunits and two beta subunits. The encoded protein is one of several different types of alpha subunits and provides the major part of the catalytic site of the active enzyme. In collagen and related proteins, prolyl 4-hydroxylase catalyzes the formation of 4-hydroxyproline that is essential to the proper three-dimensional folding of newly synthesized procollagen chains. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2014 ...
The protein composition of Collagen Type I & III consist of nineteen amino acids responsible for growth and maintenance of the body with unusually high percentages of glycine, proline, hydroxyproline, all of which are found in the skin and muscle. This is what make Collagen M.D. Advanced Connective Tissue Formula effective in those areas. ...
Collagen is a protein made up of amino-acids, which are in turn built of carbon, oxygen and hydrogen. Collagen contains specific amino acids - Glycine, Proline, Hydroxyproline and Arginine.
Although the lung can regenerate after injury, repeated injury results in fibrosis, which compromises respiratory function. Cao et al. injected damaging chemicals into the tracheas of mice to investigate how fibrosis due to repetitive injury occurred. Mice receiving a single injection recovered blood oxygen concentration and exhibited re-epithelialization by type 1 alveolar cells and proliferation of the surfactant-producing type 2 alveolar epithelial cells. However, mice receiving repeated injections did not and, instead, had the sustained presence of protein markers of fibrosis (α-smooth muscle actin, collagen I, and hydroxyproline). Examination of Notch signaling, a regulator of lung fibroblasts, using transgenic Notch reporter mice revealed that Notch activation occurred in perivascular fibroblasts after repetitive injury. Repetitive lung injury resulted in an increase in the abundance of the Notch ligand Jagged1 (Jag1) in the pulmonary capillary endothelial cells (PCECs), which function ...
This product is rich in 18 kinds of amino acids human body required, including hydroxyproline, glycine, proline, which serves as function like protecting skins but can not be synthesized by our
antiarrhythmic peptide: atrial peptide from bovine atria; hexapeptide composed of hydroxyproline(1), proline(1), glycine(3) & alanine(1); both N-terminal & carboxy-terminal residues are glycine
Alpha Visage affects Face Wash Clean Face. I presume I need to find multitudes who have same interest in Alpha Visage. Each company has a different Alpha Visage scale and for that matter, a different way of Alpha Visage. Its the conclusion: There is very a bit implausible with what I am telling yo
|p||strong|SYIS AMPULKY PURE COLLAGEN 100% 10x3ML|/strong||/p| |p|Ampulka Collagen Pure 100% obsahuje |strong|rozpustný kolagén - soluble collagen|/strong| – ktorý zadržiava vodu v pokožke a chráni ju pred vznikom vr&aac
A colorimetric, soluble collagen assay for the analysis of acid and pepsin-soluble collagens produced in situ or during in-vitro cell culture.
Hydroxyproline. This imino acid differs from proline due to a hydroxyl group on carbon 4. Hypusine. This amino acid is obtained ... and in hydroxyproline the hydroxylation of proline is critical for maintaining connective tissues. Another example is the ...
Adams E, Goldstone A (December 1960). "Hydroxyproline metabolism. IV. Enzymatic synthesis of gamma-hydroxyglutamate from Delta ...
"Hydroxyproline metabolism. III. Enzymatic synthesis of hydroxyproline from Delta1-pyrroline-3-hydroxy-5-carboxylate". J. Biol. ...
The organic compound citrulline is an α-amino acid. Its name is derived from citrullus, the Latin word for watermelon, from which it was first isolated in 1914 by Koga and Odake.[2] It was finally identified by Wada in 1930.[3] It has the formula H2NC(O)NH(CH2)3CH(NH2)CO2H. It is a key intermediate in the urea cycle, the pathway by which mammals excrete ammonia by converting it into urea. Citrulline is also produced as a byproduct of the enzymatic production of nitric oxide from the amino acid arginine, catalyzed by nitric oxide synthase.[4] ...
Besides the nervous system, GABA is also produced at relatively high levels in the insulin-producing β-cells of the pancreas. The β-cells secrete GABA along with insulin and the GABA binds to GABA receptors on the neighboring islet α-cells and inhibits them from secreting glucagon (which would counteract insulin's effects).[25] GABA can promote the replication and survival of β-cells[26][27][28] and also promote the conversion of α-cells to β-cells, which may lead to new treatments for diabetes.[29] GABA has also been detected in other peripheral tissues including intestines, stomach, Fallopian tubes, uterus, ovaries, testes, kidneys, urinary bladder, the lungs and liver, albeit at much lower levels than in neurons or β-cells. GABAergic mechanisms have been demonstrated in various peripheral tissues and organs, which include the intestines, the stomach, the pancreas, the Fallopian tubes, the uterus, the ovaries, the testes, the kidneys, the urinary bladder, the lungs, and the liver.[30] ...
Amino acid supplements, including L-ornithine, are frequently marketed to bodybuilders and weightlifters with claims for increasing levels of human growth hormone (HGH), muscle mass and strength. A clinical study reported that L-ornithine at 2 g/d did not increase HGH.[6] A review on the topic concluded "The use of specific amino acids to stimulate GH release by athletes is not recommended."[7] ...
Neurons that produce GABA as their output are called GABAergic neurons, and have chiefly inhibitory action at receptors in the adult vertebrate. Medium spiny cells are a typical example of inhibitory central nervous system GABAergic cells. In contrast, GABA exhibits both excitatory and inhibitory actions in insects, mediating muscle activation at synapses between nerves and muscle cells, and also the stimulation of certain glands.[4] In mammals, some GABAergic neurons, such as chandelier cells, are also able to excite their glutamatergic counterparts.[5] GABAA receptors are ligand-activated chloride channels: when activated by GABA, they allow the flow of chloride ions across the membrane of the cell. Whether this chloride flow is depolarizing (makes the voltage across the cell's membrane less negative), shunting (has no effect on the cell's membrane potential), or inhibitory/hyperpolarizing (makes the cell's membrane more negative) depends on the direction of the flow of chloride. When net ...
Specific sets of hydroxyproline O-β-galactosyltransferases, β-1,3-galactosyltransferases, β-1,6-galactosyltransferases, α- ... Proline residues in the protein backbone can be hydroxylated to Hydroxyproline (O) depending on the surrounding amino acids. ... Ogawa-Ohnishi, Mari; Matsubayashi, Yoshikatsu (2015-03-01). "Identification of three potent hydroxyproline O- ... and Analysis of Hydroxyproline-Rich Glycoproteins". Plant Physiology. 153 (2): 485-513. doi:10.1104/pp.110.156554. ISSN 0032- ...
1974). "Defective hydroxyproline metabolism in type II hyperprolinemia". Biochemical Medicine. 10 (4): 329-36. doi:10.1016/0006 ... "Genetic evidence for a common enzyme catalyzing the second step in the degradation of proline and hydroxyproline". J. Clin. ...
"Entrez Gene: TULP1 tubby like protein 1". Powles TJ, Rosset G, Leese CL, Bondy PK (1977). "Early morning hydroxyproline ...
The protein is one of the enzymes (4-hydroxy-2-oxoglutarate aldolase) involved in metabolism of hydroxyproline to glyoxylate. ... The enzyme overactivity can form excessive glyoxylate from hydroxyproline. Glyoxylate is catabolised to oxalate, resulting in ...
The core-specific lectin contains hydroxyproline, hydroxylysine, and glucosylgalactosylhydroxylysine residues". J Biol Chem. ...
Collagen consists of both 3‐hydroxyproline and 4‐hydroxyproline residues. Hydroxylation occurs at the γ-C atom, forming ... This is due to the fact that collagen makes up about 25-35% of the protein in our bodies and contains a hydroxyproline at ... hydroxyproline (Hyp), which stabilizes the secondary structure of collagen due to the strong electronegative effects of oxygen ...
Two amino acids are alkanolamines, formally speaking: serine and hydroxyproline. Veratridine and veratrine Tropane alkaloids ...
Min JH, Yang H, Ivan M, Gertler F, Kaelin WG, Pavletich NP (2002). "Structure of an HIF-1alpha -pVHL complex: hydroxyproline ... hydroxyproline recognition in signaling". Science. 296 (5574): 1886-9. Bibcode:2002Sci...296.1886M. doi:10.1126/science.1073440 ... "Structural basis for the recognition of hydroxyproline in HIF-1 alpha by pVHL". Nature. 417 (6892): 975-8. Bibcode:2002Natur. ...
Pyridinoline Elevated levels of serum and urinary hydroxyproline are also found. Bone scans are useful in determining the ...
Min JH, Yang H, Ivan M, Gertler F, Kaelin WG, Pavletich NP (2002). "Structure of an HIF-1alpha -pVHL complex: hydroxyproline ...
... hydroxyproline recognition in signaling". Science. 296 (5574): 1886-9. Bibcode:2002Sci...296.1886M. doi:10.1126/science.1073440 ... "Structural basis for the recognition of hydroxyproline in HIF-1 alpha by pVHL". Nature. 417 (6892): 975-8. doi:10.1038/ ...
... hydroxyproline recognition in signaling". Science. 296 (5574): 1886-9. doi:10.1126/science.1073440. PMID 12004076. S2CID ...
Kato H, Matsumura Y, Maeda H (May 1988). "Isolation and identification of hydroxyproline analogues of bradykinin in human urine ...
Wheat cells also release hydroxyproline glycoprotein (HRGP) in their cell walls. Secretion of HRGP is dependent on the signal ...
It was found that the elastic, collagen, and hydroxyproline components were higher in upper abdomen regions compared to lower ... The elastic, collagen, and hydroxyproline components were sampled and then studied. ...
This enzyme catalyses the following chemical reaction Hydrolysis of Xaa!Pro dipeptides; also acts on aminoacyl-hydroxyproline ...
Lower proline and hydroxyproline contents are characteristic of cold-water, but not warm-water fish; the latter tend to have ... Proline or hydroxyproline constitute about 1/6 of the total sequence. With glycine accounting for the 1/3 of the sequence, this ... The most common motifs in the amino acid sequence of collagen are glycine-proline-X and glycine-X-hydroxyproline, where X is ... Hydroxyproline derived from proline Hydroxylysine derived from lysine - depending on the type of collagen, varying numbers of ...
... hydroxyproline recognition in signaling". Science. 296 (5574): 1886-9. doi:10.1126/science.1073440. PMID 12004076. S2CID ...
... hydroxyproline recognition in signaling". Science. 296 (5574): 1886-9. Bibcode:2002Sci...296.1886M. doi:10.1126/science.1073440 ...
2002). "Structure of an HIF-1alpha -pVHL complex: hydroxyproline recognition in signaling". Science. 296 (5574): 1886-9. ...
Hydroxyproline-rich glycopeptides were found in tobacco in 2001 and AtPEPs (Arabidopsis thaliana Plant Elicitor Peptides) were ... The precursors to HypSys appear to represent a distinct subfamily of hydroxyproline-rich proteins found in cell walls. Upon ... Pearce G, Bhattacharya R, Chen YC, Barona G, Yamaguchi Y, Ryan CA (2009). "Isolation and characterization of hydroxyproline- ... Pearce, G.; Siems, W.; Bhattacharya, R.; Chen, Y.; Ryan, C. (2007). "Three hydroxyproline-rich glycopeptides derived from a ...
Hydroxyproline derived from proline. *Hydroxylysine derived from lysine - depending on the type of collagen, varying numbers of ... Lower proline and hydroxyproline contents are characteristic of cold-water, but not warm-water fish; the latter tend to have ... The most common motifs in the amino acid sequence of collagen are glycine-proline-X and glycine-X-hydroxyproline, where X is ... From the blood, the peptides (containing hydroxyproline) are transported into the target tissues (e.g., skin, bones, and ...
... and hydroxyproline-rich proteins of the Chlamydomonas cell wall; also cleaves azocasein, gelatin and Leu-Trp-Met-Arg-Phe-Ala ... hydroxy)proline-specific proteases". European Journal of Biochemistry. 170 (1-2): 485-91. doi:10.1111/j.1432-1033.1987.tb13725. ...
2S,4R)-4-Hydroxyproline, or L-hydroxyproline (C5H9O3N), is an amino acid, abbreviated as Hyp or O, e.g., in Protein Data Bank. ... Hydroxyproline is also found in the walls of oomycetes, fungus-like protists related to diatoms. (2S,4S)-cis-4-Hydroxyproline ... Hydroxyproline differs from proline by the presence of a hydroxyl (OH) group attached to the gamma carbon atom. Hydroxyproline ... Hydroxyproline is found in few proteins other than collagen. For this reason, hydroxyproline content has been used as an ...
Other names in common use include hydroxyproline epimerase, hydroxyproline 2-epimerase, and L-hydroxyproline epimerase. This ... In enzymology, a 4-hydroxyproline epimerase (EC 5.1.1.8) is an enzyme that catalyzes the chemical reaction trans-4-hydroxy-L- ... Adams E, Norton IL (May 1964). "Purification and properties of inducible hydroxyproline 2-epimerase from Pseudomonas". The ... The systematic name of this enzyme class is 4-hydroxyproline 2-epimerase. ...
Products containing HYDROXYPROLINE made by company: Apriori Beauty There are no products in the database that match your ...
Rabbit polyclonal Hydroxyproline antibody validated for WB, IHC. Referenced in 3 publications and 3 independent reviews. ... This antibody targets conjugated Trans-Hydroxyproline, it does not recognize free Trans-Hydroxyproline. ... Hydroxyproline, a non-essential amino acid derived from proline, with no known therapeutic use. Hydroxproline is used as a ... Anti-Hydroxyproline antibody (ab37067) at 1/500 dilution + lysate prepared from HEK293T cells at 1 µg. Secondary. HRP- ...
L-4-hydroxyproline EC Number:. 200-091-9. EC Name:. L-4-hydroxyproline. CAS Number:. 51-35-4 Molecular formula:. C5H9NO3 IUPAC ... L-4-hydroxyprolinehydroxyproline. CAS Number:. 51-35-4. Molecular formula:. C5H9NO3. ... L-4-hydroxyprolinehydroxyproline. CAS Number:. 51-35-4. Molecular formula:. C5H9NO3. ... L-4-hydroxyprolinehydroxyproline. CAS Number:. 51-35-4. Molecular formula:. C5H9NO3. ...
The PDB archive contains information about experimentally-determined structures of proteins, nucleic acids, and complex assemblies. As a member of the wwPDB, the RCSB PDB curates and annotates PDB data according to agreed upon standards. The RCSB PDB also provides a variety of tools and resources. Users can perform simple and advanced searches based on annotations relating to sequence, structure and function. These molecules are visualized, downloaded, and analyzed by users who range from students to specialized scientists.
Decrease in urinary hydroxyproline and serum calcium; Failure of three patients to decrease total hydroxyproline; Clinical and ... Hydroxyproline Excretion in Patients with Breast Cancer and Response to Treatment. Powles, T.J.; Leese, C.L.; Bondy, P.K. // ... Urinary hydroxyproline as a Non-Invasive Biomarker for diagnosing Osteofluorosis in Bovines. Gupta, A. R.; Dey, S.; Saini, M.; ... Urinary hydroxyproline an index for bone metabolism was determined in cattle from fluoride endemic area. Cattle (n=12) were ...
Hydroxyproline metabolism in mouse models of primary hyperoxaluria.. [John Knight, Ross P Holmes, Scott D Cramer, Tatsuya ... Hydroxyproline (Hyp) catabolism, which occurs mainly in the liver and kidney, is a prominent source of glyoxylate and could ...
I am estimating hydroxyproline in urine by Neuman metod. I have a problem in estimating hydroxyproline. I took all the care ... 1. Collagen content calculations from hydroxy proline assay:. g of hydroxy proline/ml X dilution factor (if any) X conversion ... than in making the basic standards from hydroxyproline. I ordered poly-l-hydroxyproline, but it simply wouldnt go into ... Briefly, hydroxyproline std conc 10 ug/ml, 20 ug/ml, 30 ug/ml and blank 0.0001 N HCl. One ml of each std / blank is reacted ...
SYNTHESIS OF HYDROXYPROLINE IN VITRO BY THE HYDROXYLATION OF PROLINE IN A PRECURSOR OF COLLAGEN. Darwin J. Prockop and Kale ... SYNTHESIS OF HYDROXYPROLINE IN VITRO BY THE HYDROXYLATION OF PROLINE IN A PRECURSOR OF COLLAGEN ... SYNTHESIS OF HYDROXYPROLINE IN VITRO BY THE HYDROXYLATION OF PROLINE IN A PRECURSOR OF COLLAGEN ... SYNTHESIS OF HYDROXYPROLINE IN VITRO BY THE HYDROXYLATION OF PROLINE IN A PRECURSOR OF COLLAGEN ...
hydroxyproline. by blazing » Tue Oct 18, 2011 6:53 am Can the 4-hyydroxyproline measure insoluble crosslink collagen content in ... hydroxyproline. Discussion of all aspects of biological molecules, biochemical processes and laboratory procedures in the field ... Insoluble cross-linked collagen can be measured by determination of the 4-hydroxyproline upon hydrolysis. There are now a few ... collagen composition was measured by hydroxyproline assay as soluble collagen (1 M NaCl extractable) versus insoluble collagen ...
... and Hydroxyproline Polypeptides in Human Neutrophils," Mediators of Inflammation, vol. 2005, no. 1, pp. 31-38, 2005. doi: ...
where to buy 51-35-4(L-Hydroxyproline).Also offer free database of 51-35-4(L-Hydroxyproline) including MSDS sheet(poisoning, ... Product Name: L-Hydroxyproline Synonyms: 4-Hydroxyproline;4-L-Hydroxyproline;delta-Hydroxyproline;Hypro;L-Proline, 4-hydroxy-, ... Product L-Hydroxyproline TNJ is a chemical company since 2001. Our specialization, your confidence-we keep committing to meet ... L-Hydroxyproline 1) Quick Response Within 12 hours; 2) Quality Guarantee: All products are strictly tested by our QC, confirmed ...
Hydroxyproline (Hyp) amino acid. Essential component of collagen. Stylized skeletal formula (chemical structure). Atoms are ... Caption: Hydroxyproline (Hyp) amino acid. Essential component of collagen. Stylized skeletal formula (chemical structure). ... hydroxyproline, hyp, icon, line, model, molecular, molecule, nitrogen, oxygen, proline, prolyl, ring, skeletal, structural, ...
This protein is highly basic and contains large amounts of lysine, dopa, and 3- and 4-hydroxyproline. The composition of this ... Polyphenolic Substance of Mytilus edulis: Novel Adhesive Containing L-Dopa and Hydroxyproline ... Polyphenolic Substance of Mytilus edulis: Novel Adhesive Containing L-Dopa and Hydroxyproline ... Polyphenolic Substance of Mytilus edulis: Novel Adhesive Containing L-Dopa and Hydroxyproline ...
4-hydroxy-2-pyrrolidinecarboxylic acid, a heterocyclic amino... Explanation of Hydroxyproline ... Find out information about Hydroxyproline. C5H9O3N An amino acid that is essentially limited to structural proteins of the ... Hydroxyproline. Also found in: Dictionary, Thesaurus, Medical, Wikipedia. hydroxyproline. [hī¦dräk·sə′prō‚lēn] (biochemistry) C ... Hydroxyproline , Article about Hydroxyproline by The Free Dictionary https://encyclopedia2.thefreedictionary.com/Hydroxyproline ...
These statements have not been evaluated by the Food and Drug Administration. This product is not intended to diagnose, treat, cure or prevent any disease.. ...
Hydroxyproline is a part of normal diets. As examples, meats and gelatin-containing foods have large amounts of hydroxyproline ... Hydroxyproline is an amino acid, a substance that is found naturally in our bodies, especially in collagen that is needed for ... How much hydroxyproline is broken down into oxalate and glycolate and eliminated by the kidneys into the urine? ... Hydroxyproline is broken down in the body and becomes oxalate and glycolate that is eliminated in the urine. ...
Crystal structure of a hydroxyproline epimerase from agrobacterium vitis, target efi-506420, with bound trans-4-oh-l-proline ... 4-HYDROXYPROLINE. HYDROXYPROLINE. C5 H9 N O3. PMMYEEVYMWASQN-DMTCNVIQSA-N. ... Crystal structure of a hydroxyproline epimerase from agrobacterium vitis, target efi-506420, with bound trans-4-oh-l-proline. * ... Crystal structure of a hydroxyproline epimerase from agrobacterium vitis, target efi-506420, with bound trans-4-oh-l-proline. ...
These results demonstrated that the content of amino acid (especially hydroxyproline and cysteine) had a synergistic effect on ... Synergistic effect of hydroxyproline and cysteine Z. Bao, Y. Sun, K. Rai, X. Peng, S. Wang, R. Nian and M. Xian, Biomater. Sci. ... The content of hydroxyproline was 13.4% in TC, which was similar with that in porcine collagen (PC, 13.6%) and higher than that ...
... and 4-hydroxyproline (an imino acid; also often referred to as an amino acid) were discovered in cattle, and the discovery of ... Important roles of dietary taurine, creatine, carnosine, anserine and 4-hydroxyproline in human nutrition and health Amino ... Of particular note, taurine, carnosine, anserine, and creatine are absent from plants, and hydroxyproline is negligible in many ... and 4-hydroxyproline (an imino acid; also often referred to as an amino acid) were discovered in cattle, and the discovery of ...
Hydroxyproline Colorimetric Assay Kit can be used for research. ... Hydroxyproline Assay Kit is designed to measure hydroxyproline ... Purified Hydroxyproline Colorimetric Assay Kit from Creative Biomart. ... Hydroxyproline Assay Kit (Colorimetric) is designed to measure hydroxyproline.. Description:. Hydroxyproline (4-hydroxyproline ... Hydroxyproline Colorimetric Assay Kit. Download Datasheet See All Hyp Related Kits Bring this labeled protein directly to your ...
As a specific amino acid in collagen, the detection of l-hydroxyproline (l-Hyp) can be used to indicate whether the hydrolyzed ... As a specific amino acid in collagen, the detection of L-hydroxyproline (L-Hyp) can be used to indicate whether the hydrolyzed ... Sensitive determination of L-hydroxyproline in dairy products by capillary electrophoresis with in-capillary optical fiber ... Sensitive determination of L-hydroxyproline in dairy products by capillary electrophoresis with in-capillary optical fiber ...
An analytical method was developed and validated for the measurement of hydroxyproline (Hyp) levels in mouse kidney by high- ... Bioanalytical Method for the Determination of Hydroxyproline in Mouse Kidney by High-Performance Liquid Chromatography with ...
I am trying to analyse the hydroxyproline to determine collagen content in rat heart samples, I am following Reddy and Enwemeka ... Hydroxyproline Standard - 1-5 µg/2 0.001N HCl ml.. Buffer-0.5 g citric acid monohydrate, 0.12 ml glacial acetic acid, 0.82 g ... Hydroxyproline oxidation was initiated by adding 50ul chloramine T solution to each well. Agitate and let stand for 20 minutes ... hydroxyproline assay to determine collagen content. Discussion of all aspects of biological molecules, biochemical processes ...
Hydroxyproline Excretion and Height Velocity in Adolescent Boys Message subject: (Your Name) has forwarded a page to you from ...
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The hydroxyproline is present as an hydroxyproline-rich cell wall glycoprotein. The protein is rich in hydroxyproline (36%), ... A Developmentally Regulated Hydroxyproline-Rich Glycoprotein from the Cell Walls of Soybean Seed Coats. Gladys I. Cassab, Jorge ... We determined the ratio of hydroxyproline to dry weight in a number of tissues within the seed; however, only the seed coat ... In soybean seeds the level of hydroxyproline is regulated in a developmental and tissue-specific manner. The seed coat contains ...
The location of arabinosyl:hydroxyproline transferase in the membrane system of potato tissue culture cells R J Owens; R J ... The results are discussed in relation to the synthesis of the hydroxy-proline-rich glycoprotein component of plant cell walls. ... R J Owens, D H Northcote; The location of arabinosyl:hydroxyproline transferase in the membrane system of potato tissue culture ... arabinose yielded a glycoprotein fraction containing labelled material with the characteristics of hydroxyproline arabinosides ...
Administration of L-hydroxyproline caused a large increase in urinary hydroxyproline excretion, as expected (Table 2). Recovery ... Lowry M, Hall DE, Brosnan JT: Hydroxyproline metabolism by the rat kidney: Distribution of renal enzymes of hydroxyproline ... Figure 1. Catabolic pathway of L-hydroxyproline. AspAT, aspartate aminotransferase; DH, dehydrogenase; Hyp, hydroxyproline; OAA ... L-hydroxyproline to oxalate plus glycolate was only 0.36%. The increase in oxalate excretion due to L-hydroxyproline ...
  • The seed coat contains approximately 77% of the total hydroxyproline in the seed at all stages of development. (plantphysiol.org)
  • Relation of urinary total hydroxyproline: Creatinine ratio to height velocity in children with retarded growth. (semanticscholar.org)
  • Urinary total hydroxyproline: creatinine ratio. (semanticscholar.org)
  • Increased serum and urine levels of hydroxyproline have also been demonstrated in Paget's disease. (wikipedia.org)
  • How much hydroxyproline is broken down into oxalate and glycolate and eliminated by the kidneys into the urine? (rarediseasesnetwork.org)
  • Hydroxyproline is broken down in the body and becomes oxalate and glycolate that is eliminated in the urine. (rarediseasesnetwork.org)
  • This study is being done to determine how much the metabolism of hydroxyproline contributes to oxalate and glycolate in the urine of patients with primary hyperoxaluria. (rarediseasesnetwork.org)
  • Blood and urine samples will be taken before, during and after the infusion in order to check the patient's levels of hydroxyproline, oxalate and glycolate. (rarediseasesnetwork.org)
  • A variety of disease states are believed to affect collagen turnover and can cause elevated serum or urine hydroxyproline. (creativebiomart.net)
  • It can be used to measure hydroxyproline from other biological samples such as serum or urine if they have undergone a prior purification process. (creativebiomart.net)
  • Measurement of hydroxyproline in various tissues or protein/peptide hydrolysates, serum & urine. (creativebiomart.net)
  • 4-hydroxyproline (OHP) in urine have been suggested as disease biomarkers for bone turnover and osteoporosis [27]. (thefreedictionary.com)
  • The bone degradation is accompanied with an increased formation of hydroxyproline, which is released into blood circulation and excreted with urine. (recipe.de)
  • 2018. Rapid determination of free prolyl dipeptides and 4-hydroxyproline in urine using flow-gated capillary electrophoresis -- In Proceedings: 14th Annual Symposium on Graduate Research and Scholarly Projects. (wichita.edu)
  • To determine these compounds in human urine samples, we report a rapid and accurate method for the selective detection of free prolyl dipeptides and 4-hydroxyproline (secondary amines) by using flow-gated capillary electrophoresis coupled with laser-induced fluorescence (CE-LIF) detection method. (wichita.edu)
  • therefore, these results suggest that an important role of mitochondrial SPT/AGT in carnivores is to convert L-hydroxyproline-derived glyoxylate into glycine in situ , preventing undesirable overflow into the production of oxalate. (asnjournals.org)
  • The major metabolic pathway of hydroxyproline-derived glyoxylate in rat kidney has been suggested to be transamination to glycine ( 3 ). (asnjournals.org)
  • A single peptide chain of collagen ([alpha]-chain) is comprised of 33% glycine (G), 10% proline (P), and 10% hydroxyproline (HyP) in a distinctive repeating pattern of [(glycine-X-Y).sub.n], where X is frequently proline and Y is frequently 4-hydroxyproline (some 3-hydroxyproline or 5-hydroxylysine). (thefreedictionary.com)
  • The collagen in the dermis is triple-helical structure of glycine, proline and hydroxyproline. (askaliteformula.com)
  • Glycine, proline, and hydroxyproline - the three most plentiful amino acids in collagen supplements - often end up in fibroblasts, special cells in your skin and connective tissue. (naturalforce.com)
  • Human collagen is made almost entirely of glycine, proline, and hydroxyproline, in roughly the same ratio you find in collagen supplements. (naturalforce.com)
  • It's easier to digest, which means you get maximum access to glycine, proline, hydroxyproline, and the other precious amino acids in collagen. (naturalforce.com)
  • Look for those top three amino acids (glycine, proline, and hydroxyproline) on the label. (naturalforce.com)
  • Glycine / Proline and Hydroxyproline (derived from proline) are the main amino acids that make up and stabilize the constitution of collagen. (viovet.co.uk)
  • The production of collagen in the body involves vitamin C, glycine, proline, hydroxyproline and lysine. (viovet.co.uk)
  • Urinary hydroxyproline an index for bone metabolism was determined in cattle from fluoride endemic area. (ebscohost.com)
  • Hydroxyproline and Collagen Metabolism. (ebscohost.com)
  • Presents a transcription of a clinical staff conference at the Clinical Center in Bethesda, Maryland, about the clinical implications of hydroxyproline and collagen metabolism. (ebscohost.com)
  • Hydroxyproline metabolism in mouse models of primary hyperoxaluria. (sigmaaldrich.com)
  • For this purpose, we investigated ( 1 ) the effect of glucagon on enzymes involved in L-hydroxyproline metabolism (experiment 1), and ( 2 ) the effect of glucagon induction of mitochondrial SPT/AGT on oxalate formation from L-hydroxyproline and that from glycolate in vivo (experiment 2). (asnjournals.org)
  • Proline metabolism was markedly changed in HCC tumor tissue, characterized with accelerated consumption of proline and accumulation of hydroxyproline, which significantly correlated with α-fetoprotein levels and poor prognosis in HCC. (aacrjournals.org)
  • Title: Modified assay for determination of hydroxyproline in a tissue hydrolyzate. (scientistsolutions.com)
  • A modified assay for the determination of hydroxyproline in tissue is presented. (scientistsolutions.com)
  • Insoluble cross-linked collagen can be measured by determination of the 4-hydroxyproline upon hydrolysis. (biology-online.org)
  • Right lungs were excised for hydroxyproline determination via the chloramine-T spectro-photometric absorbance assay as previously described (Fattman et al. (thefreedictionary.com)
  • Bioanalytical Method for the Determination of Hydroxyproline in Mouse Kidney by High-Performance Liquid Chromatography with Tandem Mass Spectrometric Detection. (sigmaaldrich.com)
  • Determination of 4-hydroxyproline was performed according to the procedure suggested by Kindt et al. (thefreedictionary.com)
  • The severity of pulmonary fibrosis was assessed by histopathologic evaluation and determination of hydroxyproline levels. (aspetjournals.org)
  • Determination of the subject material of hydroxyproline in liver tissue is regarded as a superior system to quantify fibrosis and to evaluate the effectiveness of new possibly antifibrotic agents. (thrombininhibitors.com)
  • The hydroxyproline is present as an hydroxyproline-rich cell wall glycoprotein. (plantphysiol.org)
  • Incubation of a particulate preparation from potato tissue culture cells with UDP-beta-L-[1-3H] arabinose yielded a glycoprotein fraction containing labelled material with the characteristics of hydroxyproline arabinosides. (portlandpress.com)
  • Here we report on the properties of extensin-2, a second salt-extractable hydroxyproline-rich glycoprotein from carrot. (niu.edu)
  • L-Hydroxyproline is detected in such hydroxyproline-rich glycoprotein of plant cell walls. (intechopen.com)
  • Identification of plant hydroxyproline rich glycoprotein sequences by performing sequence bias and motif search, prediction of hydroxylated proline residues and scraping relevant data from web based prediction services. (rdrr.io)
  • Hydroxyproline is produced by hydroxylation of the amino acid proline by the enzyme prolyl hydroxylase following protein synthesis (as a post-translational modification). (wikipedia.org)
  • Hydroxyproline is a major component of the protein collagen, comprising roughly 13.5% of mammalian collagen. (wikipedia.org)
  • This protein is highly basic and contains large amounts of lysine, dopa, and 3- and 4-hydroxyproline. (sciencemag.org)
  • The formation of L -hydroxyproline in living cells is effected by hydroxylation of the proline bonded to the protein components (the O atom of the hydroxyl group is incorporated into hydroxyproline upon fixation of molecular O 2 ). (thefreedictionary.com)
  • Hydroxyproline Assay Kit is designed to measure hydroxyproline in tissue or protein/peptide hydrolysates. (creativebiomart.net)
  • The sugar-protein linkage was resistant to hot alkaline hydrolysis, and the hydrolytic products showed similar electrophoretic and chromatographic behavior to authentic hydroxyproline-arabinosides prepared from potato tissue culture cell walls. (portlandpress.com)
  • Mitochondrial production of glyoxylate from hydroxyproline is assumed to be significant in carnivores, because the hydroxyproline content of collagen is about 10 to 13% ( 4 ) and collagen accounts for about 30% of total animal protein. (asnjournals.org)
  • Genes encoding hydroxyproline-rich glycoproteins (HRGPs) in green organisms, key protein constituents of the cell wall, carry short-repeat modules that are posited to specify proline hydroxylation and/or glycosylation events. (plantphysiol.org)
  • Hydroxyproline is the major building block of collagen which is regarded as one of the most important biomaterials [1] because of its widespread occurrence as structural protein in vertebrates [2]. (scienomics.com)
  • Hydroxyproline is necessary for the construction of the body's major structural protein, collagen. (healthmatters.io)
  • 4-Hydroxyproline, or hydroxyproline (C5H9O3N), is an uncommon amino acid, abbreviated as HYP, e.g., in Protein Data Bank.Hydroxyproline differs from proline by the presence of a hydroxyl (OH) group attached to the C (gamma) atom. (cloud-clone.com)
  • At the same time, the fruit extract effectively lowered the iron-overload induced raised levels of lipid per oxidation, protein carbonyl, hydroxyproline and liver iron by 49%, 67%, 67% and 26%, respectively, with oral treatment of 200 mg/kg b.w. dose of TCME. (biomedcentral.com)
  • Hydroxyproline rich glycoproteins (HRGPs) are also found in plant cell walls. (wikipedia.org)
  • Developmental localization and the role of hydroxyproline rich glycoproteins during somatic embryogenesis of banana (Musa spp. (ebscohost.com)
  • Effect of Alcohol on Total Urinary Hydroxyproline Excretion. (ebscohost.com)
  • Presents a study regarding the effect of alcohol on total urinary hydroxyproline excretion. (ebscohost.com)
  • Total urinary hydroxyproline excretion in a number of alcoholics while drinking and then withdrawn from alcohol. (ebscohost.com)
  • Intrauterine malnutrition and urinary hydroxyproline excretion. (semanticscholar.org)
  • Although it is not directly incorporated into proteins, hydroxyproline comprises roughly 4% of all amino acids found in animal tissue, an amount greater than seven other amino acids that are translationally incorporated. (wikipedia.org)
  • In addition to collagen, the mammalian proteins elastin and argonaute 2 have collagen-like domains in which hydroxyproline is formed. (wikipedia.org)
  • Hydroxyproline is found in few proteins other than collagen. (wikipedia.org)
  • other proteins contain negligible amounts of L -hydroxyproline or none at all. (thefreedictionary.com)
  • As a specific amino acid in collagen, the detection of L -hydroxyproline ( L -Hyp) can be used to indicate whether the hydrolyzed leather proteins are added to dairy products or not. (rsc.org)
  • This is a peptide sourced from natural proteins and mimics the endogenous collagen forming amino acid version hydroxyproline. (askaliteformula.com)
  • Transfers galactose from UDP-galactose to hydroxyproline residues in the arabinogalactan proteins (AGPs). (icr.ac.uk)
  • The addition of galactose onto the peptidyl hydroxyproline residues in AGP core proteins represents the first committed step in arabinogalactan polysaccharide addition. (icr.ac.uk)
  • Could a reduction in the amount of hydroxyproline in the diet be of benefit to PH patients? (rarediseasesnetwork.org)
  • This assay estimates the amount of hydroxyproline, which has been shown to account for approximately 14% the amino acids in collagen. (biology-online.org)
  • Focuses on the use of measured urinary excretion of hydroxyproline in monitoring the progression of metastatic breast cancer in Great Britain. (ebscohost.com)
  • Urinary excretion of hydroxyproline in workers occupationally exposed to vibration. (semanticscholar.org)
  • For this reason, hydroxyproline content has been used as an indicator to determine collagen and/or gelatin amount. (wikipedia.org)
  • Hi, I am trying to analyse the hydroxyproline to determine collagen content in rat heart samples, I am following Reddy and Enwemeka 1996 paper but unable to get a proper standard curve, even my blank (water) looks similar to my highest standard concentration (2--0ug). (biology-online.org)
  • Synthetic peptide corresponding to Hydroxyproline conjugated to Bovine Serum Albumin (BSA). (abcam.com)
  • Increase of urinary and serum hydroxyproline in subjects exposed to cadmium. (ebscohost.com)
  • The article presents a study which examines the increased concentrations of urinary and serum hydroxyproline resulting from cadmium (Cd) exposure. (ebscohost.com)
  • The effects of high fat diet (HFD) on the hydroxyproline (Hyp) fractions in serum and aortic tissues of rabbits and collagen content in the aortic tissues of rabbits have not been documented before. (biomedcentral.com)
  • Biochemical assay showed serum ALT actions, serum AST actions, and information screening compounds of hepatic hydroxyproline had been markedly greater in rats injected with CCl4 for 12 wk, that are consistent using the histological observations. (thrombininhibitors.com)
  • Emodin could reduce the scores of hepatic fibrosis grading, inhibit the ALT and AST activities in serum and lowered the articles of hepatic hydroxyproline. (thrombininhibitors.com)
  • It was subsequently shown that the increase in stability is primarily through stereoelectronic effects and that hydration of the hydroxyproline residues provides little or no additional stability. (wikipedia.org)
  • The arabinose residues are attached to hydroxyproline mostly in the form of trisaccharides. (plantphysiol.org)
  • Collagen molecules have a triple-helical structure and the presence of 4-hydroxyproline resulting from a posttranslational modification of peptide-bound prolyl residues provides a distinctive marker of these molecules [12]. (thefreedictionary.com)
  • Is specific for AGPs containing non-contiguous peptidyl hydroxyproline residues. (icr.ac.uk)
  • Hydroxyproline, a non-essential amino acid derived from proline, with no known therapeutic use. (abcam.com)
  • Vitiman C is required for the conversion process from proline to hydroxyproline, a deficincy in vitiman C can lead to defects in collagen synthesis, thus, resulting in easy bruising, internal bleeding, breakdown of connective tissue of the ligaments and tendons, and increased risk to blood vessel damage. (abcam.com)
  • These modifications include a change in the technique for tissue hydrolysis and a change in the preparation of the hydroxyproline oxidizing agent. (scientistsolutions.com)
  • Hydroxyproline in tissue hydrolysates is a direct measure of the amount of collagen or gelatin present. (creativebiomart.net)
  • In soybean seeds the level of hydroxyproline is regulated in a developmental and tissue-specific manner. (plantphysiol.org)
  • Hydroxyproline (hydroxyproline), is one of the imino acids, usually with a hydroxyl group in the fourth position, but sometimes also in the third position. (grantprintinginc.com)
  • An analytical method was developed and validated for the measurement of hydroxyproline (Hyp) levels in mouse kidney by high-performance liquid chromatography with tandem mass spectrometric detection (LC/MS/MS) using an analytical column specially designed for the LC/MS/MS analysis for intact amino acids. (sigmaaldrich.com)
  • Measurement of the fasting urinary hydroxyproline: creatinine ratio in normal adults and its variation with age and sex. (semanticscholar.org)
  • The RECIPE Complete Kit provides an accurate hydroxyproline measurement. (recipe.de)
  • Hydroxyproline levels are a measurement of fibrosis in the skeletal muscle. (jax.org)
  • Hydroxyproline oxidation was initiated by adding 50ul chloramine T solution to each well. (biology-online.org)
  • In the present study, we examined whether mitochondrial SPT/AGT plays a role in removing hydroxyproline-derived glyoxylate from oxidation to oxalate using rats with or without glucagon induction of liver mitochondrial SPT/AGT. (asnjournals.org)
  • The so-called dye-linked dehydrogenases catalyze the oxidation of various biomolecules in the presence of an artificial electron acceptor, in which several unique compounds related to plants as substrates such as opine(s) and L-hydroxyproline are contained. (intechopen.com)
  • Consumption of 30 g dry beef can fully meet daily physiological needs of the healthy 70-kg adult human for taurine and carnosine, and can also provide large amounts of creatine, anserine and 4-hydroxyproline to improve human nutrition and health, including metabolic, retinal, immunological, muscular, cartilage, neurological, and cardiovascular health. (nih.gov)
  • The conversion of proline to hydroxyproline requires vitamin C (ascorbic acid). (healthmatters.io)
  • Vitamin C deficiency results in the poor conversion of proline to hydroxyproline. (healthmatters.io)
  • 11] Reddy GK, Enwemeka CS, "A simplified method for the analysis of hydroxyproline in biological tissues," Clinical Biochemistry, vol. (thefreedictionary.com)
  • A simple method to determine nanogram levels of 4-hydroxyproline in biological tissues. (thefreedictionary.com)
  • Hydroxyproline helps to support the extracellular space of connective tissues in the skin. (askaliteformula.com)
  • Hydroxyproline is present in essentially all tissues and all genetic types of collagen. (healthmatters.io)
  • Are significant amounts of hydroxyproline metabolized (broken down) to oxalate in PH patients? (rarediseasesnetwork.org)
  • As examples, meats and gelatin-containing foods have large amounts of hydroxyproline. (rarediseasesnetwork.org)
  • Trans-4-Hydroxy-L-proline CAS NO:51-35-4 L-Hydroxyproline Molecular Formula:C5H9NO3 Appearance: White crystals or crystalline powder Package:25kgs/drum Application:1. (lookchem.com)
  • for example, low molecular weight Bio Cell is combined with proline hydroxyproline and vitamin C for healthy skin and hair. (thefreedictionary.com)
  • Since dye-linked dehydrogenation activity for several unique compounds related to plants as substrates including opine(s) and l -hydroxyproline had been reported in bacterial cell-free extract from 1950s to 1980s, those genetic and molecular information have only recently been elucidated [ 3 , 4 , 5 , 6 ]. (intechopen.com)
  • In enzymology, a 4-hydroxyproline epimerase (EC 5.1.1.8) is an enzyme that catalyzes the chemical reaction trans-4-hydroxy-L-proline ⇌ {\displaystyle \rightleftharpoons } cis-4-hydroxy-D-proline Hence, this enzyme has one substrate, trans-4-hydroxy-L-proline, and one product, cis-4-hydroxy-D-proline. (wikipedia.org)
  • The systematic name of this enzyme class is 4-hydroxyproline 2-epimerase. (wikipedia.org)
  • Other names in common use include hydroxyproline epimerase, hydroxyproline 2-epimerase, and L-hydroxyproline epimerase. (wikipedia.org)
  • Effect of carbimazole treatment on the hydroxyproline creatinine ratio. (ebscohost.com)
  • article{Allison1966UrinaryHC, title={Urinary hydroxyproline: Creatinine ratio of normal humans at various ages}, author={Donna J. Allison and AlexanderM. (semanticscholar.org)
  • Hydroxyproline is formed only as a post-translational modification in the peptide chain and proline hydroxylase does not hydroxylate free proline. (creativebiomart.net)
  • In the degradation pathway of bacteria, D-hydroxyproline dehydrogenase (HypDH) catalyzes the dehydrogenation reaction of cis-4-hydroxy-D-proline, and is classified into two types: homomeric and heteromeric enzymes. (intechopen.com)
  • 4-hydroxyproline (Hyp) and prolyl dipeptides such as prolyl hydroxyproline (Pro-Hyp) have been found as disease biomarkers in the diagnosis of bone turnover and osteoporosis. (wichita.edu)
  • In 1905, Hermann Leuchs synthesized a racemic mixture of 4-hydroxyproline. (wikipedia.org)
  • Because of the presence of two asymmetric carbon atoms per molecule, it has four optically active forms ( L -, D -, allo- L -, and allo- D -hydroxyprolines) and two racemic forms. (thefreedictionary.com)
  • Hydroxyproline (Hyp) catabolism, which occurs mainly in the liver and kidney, is a prominent source of glyoxylate and could account for a significant portion of the oxalate produced in PH. (sigmaaldrich.com)
  • 4-Hydroxyproline was strongly decreased in liver, muscle, and plasma. (thefreedictionary.com)
  • Hydroxyproline is a nonessential amino acid, which means that it is manufactured from other amino acids in the liver. (healthmatters.io)
  • Other hydroxyprolines also exist in nature, notably 2,3-cis 3,4-trans-dihydroxyproline, which occurs in diatom cell walls, and is postulated to have a role in silica deposition. (cloud-clone.com)
  • In the following, we present a computational study on the influence of stereoelectronic effects on the conformational properties of hydroxyproline and discuss the impact on the collagen triple-helix stability. (scienomics.com)
  • Better understanding of how the body processes hydroxyproline and oxalate may help us identify strategies to reduce oxalate production and could be beneficial for individuals with PH. (rarediseasesnetwork.org)
  • This study will also look at whether hydroxyproline is reabsorbed by the kidney and converted to glycolate and oxalate. (rarediseasesnetwork.org)
  • The present study found that urinary excretion of oxalate was markedly increased when a large dose of L-hydroxyproline or glycolate was administered to rats. (asnjournals.org)
  • Oxalate formation from L-hydroxyproline but not that from glycolate was significantly reduced when mitochondrial SPT/AGT had been induced by glucagon. (asnjournals.org)
  • LV collagen content was measured histologically by collagen volume fraction, collagen composition was measured by hydroxyproline assay as soluble collagen (1 M NaCl extractable) versus insoluble collagen (mature cross-linked), and collagen morphological structure was examined by scanning electron microscopy. (biology-online.org)
  • As hydroxyproline is a basic constituent of collagen structure, its content can serve as indicator of collagen synthesis. (thefreedictionary.com)
  • These results demonstrated that the content of amino acid (especially hydroxyproline and cysteine) had a synergistic effect on the thermal and mechanical properties of BCH, TCH and PCH, which would be an indicator for the thermal and mechanical properties of collagen hydrogels in future studies. (rsc.org)
  • One of the products formed during the conversion of L -hydroxyproline in the organism is glutamic acid. (thefreedictionary.com)
  • Pulmonary fibrosis was consistently reduced in PAR-2-deficient mice throughout the fibrotic phase, as evident from reduced Ashcroft scores (29%) and hydroxyproline levels (26%) at d 28. (springer.com)
  • The increased fibrosis in B10.mdx mice translates into an increase in hydroxyproline levels (compare mdx + Vehicle to Wild-type). (jax.org)
  • A test article that decreases fibrosis decreases the hydroxyproline levels in treated B10.mdx mice (mdx + Test Article). (jax.org)
  • Analysis of urinary hydroxyproline levels offers a marker to monitor osseous involvement in patients with metastatic malignancies. (elsevier.com)
  • A team of researchers found out that children with autistic spectrum disorder (ASD) have increased levels of the amino acid hydroxyproline. (news-medical.net)
  • Thirty-two men with stage D2 prostatic cancer were monitored by bone scan, acid and alkaline phosphatase values, and urinary hydroxyproline, beginning from 4 to 36 months after initiation of hormonal manipulation and/or systemic chemotherapy. (elsevier.com)
  • Glyoxylate formation from glycolate and that from L-hydroxyproline have been shown to occur in peroxisomes and mitochondria, respectively. (asnjournals.org)
  • The chemical reactions and pathways involving 4-hydroxyproline, C5H9NO3, a derivative of the amino acid proline. (systemsbiology.net)
  • Amino pyridine-based rhodamine conjugate (APR) has been developed as a first chemosensor for selective detection and quantification of L-4-Hydroxyproline (Hyp). (jyu.fi)