Hydrolases
Any member of the class of enzymes that catalyze the cleavage of the substrate and the addition of water to the resulting molecules, e.g., ESTERASES, glycosidases (GLYCOSIDE HYDROLASES), lipases, NUCLEOTIDASES, peptidases (PEPTIDE HYDROLASES), and phosphatases (PHOSPHORIC MONOESTER HYDROLASES). EC 3.
Epoxide Hydrolases
Carboxylic Ester Hydrolases
Lysosomes
A class of morphologically heterogeneous cytoplasmic particles in animal and plant tissues characterized by their content of hydrolytic enzymes and the structure-linked latency of these enzymes. The intracellular functions of lysosomes depend on their lytic potential. The single unit membrane of the lysosome acts as a barrier between the enzymes enclosed in the lysosome and the external substrate. The activity of the enzymes contained in lysosomes is limited or nil unless the vesicle in which they are enclosed is ruptured. Such rupture is supposed to be under metabolic (hormonal) control. (From Rieger et al., Glossary of Genetics: Classical and Molecular, 5th ed)
Acid Phosphatase
Hexosaminidases
N-Acetylmuramoyl-L-alanine Amidase
Peptide Hydrolases
Mucolipidoses
A group of inherited metabolic diseases characterized by the accumulation of excessive amounts of acid mucopolysaccharides, sphingolipids, and/or glycolipids in visceral and mesenchymal cells. Abnormal amounts of sphingolipids or glycolipids are present in neural tissue. INTELLECTUAL DISABILITY and skeletal changes, most notably dysostosis multiplex, occur frequently. (From Joynt, Clinical Neurology, 1992, Ch56, pp36-7)
Molecular Sequence Data
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
Substrate Specificity
Receptor, IGF Type 2
Amino Acid Sequence
Acetylglucosaminidase
A beta-N-Acetylhexosaminidase that catalyzes the hydrolysis of terminal, non-reducing 2-acetamido-2-deoxy-beta-glucose residues in chitobiose and higher analogs as well as in glycoproteins. Has been used widely in structural studies on bacterial cell walls and in the study of diseases such as MUCOLIPIDOSIS and various inflammatory disorders of muscle and connective tissue.
alpha-L-Fucosidase
Xylosidases
A group of enzymes that catalyze the hydrolysis of alpha- or beta-xylosidic linkages. EC 3.2.1.8 catalyzes the endo-hydrolysis of 1,4-beta-D-xylosidic linkages; EC 3.2.1.32 catalyzes the endo-hydrolysis of 1,3-beta-D-xylosidic linkages; EC 3.2.1.37 catalyzes the exo-hydrolysis of 1,4-beta-D-linkages from the non-reducing termini of xylans; and EC 3.2.1.72 catalyzes the exo-hydrolysis of 1,3-beta-D-linkages from the non-reducing termini of xylans. Other xylosidases have been identified that catalyze the hydrolysis of alpha-xylosidic bonds.
Cellulase
Cellvibrio
beta-Glucosidase
Galactosidases
beta-Mannosidase
An enzyme that catalyzes the hydrolysis of terminal, non-reducing beta-D-mannose residues in beta-D-mannosides. The enzyme plays a role in the lysosomal degradation of the N-glycosylprotein glycans. Defects in the lysosomal form of the enzyme in humans result in a buildup of mannoside intermediate metabolites and the disease BETA-MANNOSIDOSIS.
Endo-1,4-beta Xylanases
Acid Anhydride Hydrolases
Arylsulfatases
beta-N-Acetylhexosaminidases
A hexosaminidase specific for non-reducing N-acetyl-D-hexosamine residues in N-acetyl-beta-D-hexosaminides. It acts on GLUCOSIDES; GALACTOSIDES; and several OLIGOSACCHARIDES. Two specific mammalian isoenzymes of beta-N-acetylhexoaminidase are referred to as HEXOSAMINIDASE A and HEXOSAMINIDASE B. Deficiency of the type A isoenzyme causes TAY-SACHS DISEASE, while deficiency of both A and B isozymes causes SANDHOFF DISEASE. The enzyme has also been used as a tumor marker to distinguish between malignant and benign disease.
Sequence Homology, Amino Acid
Pyrophosphatases
Cathepsins
N-Glycosyl Hydrolases
Cathepsin D
Cell Wall
Monocrotophos
Cloning, Molecular
Transferases (Other Substituted Phosphate Groups)
Cellulases
A family of glycosidases that hydrolyse crystalline CELLULOSE into soluble sugar molecules. Within this family there are a variety of enzyme subtypes with differing substrate specificities that must work together to bring about complete cellulose hydrolysis. They are found in structures called CELLULOSOMES.
Catalytic Domain
Vacuoles
Sequence Alignment
The arrangement of two or more amino acid or base sequences from an organism or organisms in such a way as to align areas of the sequences sharing common properties. The degree of relatedness or homology between the sequences is predicted computationally or statistically based on weights assigned to the elements aligned between the sequences. This in turn can serve as a potential indicator of the genetic relatedness between the organisms.
Catalysis
Cellulose
A polysaccharide with glucose units linked as in CELLOBIOSE. It is the chief constituent of plant fibers, cotton being the purest natural form of the substance. As a raw material, it forms the basis for many derivatives used in chromatography, ion exchange materials, explosives manufacturing, and pharmaceutical preparations.
Hydrogen-Ion Concentration
Saposins
Acetylesterase
Cellulose 1,4-beta-Cellobiosidase
Models, Molecular
Sterol Esterase
Phosphoric Monoester Hydrolases
Glucans
alpha-Mannosidase
An enzyme that catalyzes the HYDROLYSIS of terminal, non-reducing alpha-D-mannose residues in alpha-D-mannosides. The enzyme plays a role in the processing of newly formed N-glycans and in degradation of mature GLYCOPROTEINS. There are multiple isoforms of alpha-mannosidase, each having its own specific cellular location and pH optimum. Defects in the lysosomal form of the enzyme results in a buildup of mannoside intermediate metabolites and the disease ALPHA-MANNOSIDOSIS.
alpha-Glucosidases
Enzyme Stability
Cathepsin A
Oligosaccharides
Crystallography, X-Ray
Palmitoyl-CoA Hydrolase
Neocallimastigales
Escherichia coli
A species of gram-negative, facultatively anaerobic, rod-shaped bacteria (GRAM-NEGATIVE FACULTATIVELY ANAEROBIC RODS) commonly found in the lower part of the intestine of warm-blooded animals. It is usually nonpathogenic, but some strains are known to produce DIARRHEA and pyogenic infections. Pathogenic strains (virotypes) are classified by their specific pathogenic mechanisms such as toxins (ENTEROTOXIGENIC ESCHERICHIA COLI), etc.
Alteromonas
Aspartylglucosylaminase
An enzyme that catalyzes the conversion of N(4)-(beta-N-acetyl-D-glucosaminyl)-L-asparagine and water to N-acetyl-beta-D-glucosaminylamine and L-aspartate. It acts only on asparagine-oligosaccharides containing one amino acid, i.e. the ASPARAGINE has free alpha-amino and alpha-carboxyl groups. (From Enzyme Nomenclature, 1992)
Fibrobacter
Glucan Endo-1,3-beta-D-Glucosidase
Phosphoric Diester Hydrolases
Epoxy Compounds
Sequence Analysis, DNA
Bacteriolysis
Carboxylesterase
Binding Sites
Glucan 1,4-beta-Glucosidase
Piromyces
Glycosyltransferases
Enzymes that catalyze the transfer of glycosyl groups to an acceptor. Most often another carbohydrate molecule acts as an acceptor, but inorganic phosphate can also act as an acceptor, such as in the case of PHOSPHORYLASES. Some of the enzymes in this group also catalyze hydrolysis, which can be regarded as transfer of a glycosyl group from the donor to water. Subclasses include the HEXOSYLTRANSFERASES; PENTOSYLTRANSFERASES; SIALYLTRANSFERASES; and those transferring other glycosyl groups. EC 2.4.
Carboxypeptidases
Ubiquitin Thiolesterase
Base Sequence
Leucyl Aminopeptidase
A zinc containing enzyme of the hydrolase class that catalyzes the removal of the N-terminal amino acid from most L-peptides, particularly those with N-terminal leucine residues but not those with N-terminal lysine or arginine residues. This occurs in tissue cell cytosol, with high activity in the duodenum, liver, and kidney. The activity of this enzyme is commonly assayed using a leucine arylamide chromogenic substrate such as leucyl beta-naphthylamide.
Endopeptidases
Cathepsin B
A lysosomal cysteine proteinase with a specificity similar to that of PAPAIN. The enzyme is present in a variety of tissues and is important in many physiological and pathological processes. In pathology, cathepsin B has been found to be involved in DEMYELINATION; EMPHYSEMA; RHEUMATOID ARTHRITIS, and NEOPLASM INVASIVENESS.
Trichoderma
Sphingolipid Activator Proteins
Carbohydrate Metabolism
Gold Colloid, Radioactive
Acidianus
A genus of facultatively anaerobic coccoid ARCHAEA, in the family SULFOLOBACEAE. Cells are highly irregular in shape and thermoacidophilic. Lithotrophic growth occurs aerobically via sulfur oxidation in some species. Distribution includes solfataric springs and fields, mudholes, and geothermically heated acidic marine environments.
Mannose
Comamonadaceae
Dipeptidyl-Peptidases and Tripeptidyl-Peptidases
Subcellular Fractions
Components of a cell produced by various separation techniques which, though they disrupt the delicate anatomy of a cell, preserve the structure and physiology of its functioning constituents for biochemical and ultrastructural analysis. (From Alberts et al., Molecular Biology of the Cell, 2d ed, p163)
Aminopeptidases
beta-Galactosidase
Cytophaga
Lipase
Mutation
Protein Structure, Tertiary
The level of protein structure in which combinations of secondary protein structures (alpha helices, beta sheets, loop regions, and motifs) pack together to form folded shapes called domains. Disulfide bridges between cysteines in two different parts of the polypeptide chain along with other interactions between the chains play a role in the formation and stabilization of tertiary structure. Small proteins usually consist of only one domain but larger proteins may contain a number of domains connected by segments of polypeptide chain which lack regular secondary structure.
Ruminococcus
Cellulosomes
Sucrase-Isomaltase Complex
Acidithiobacillus thiooxidans
Pectins
High molecular weight polysaccharides present in the cell walls of all plants. Pectins cement cell walls together. They are used as emulsifiers and stabilizers in the food industry. They have been tried for a variety of therapeutic uses including as antidiarrheals, where they are now generally considered ineffective, and in the treatment of hypercholesterolemia.
Chitin
Cell Fractionation
Protein Conformation
The characteristic 3-dimensional shape of a protein, including the secondary, supersecondary (motifs), tertiary (domains) and quaternary structure of the peptide chain. PROTEIN STRUCTURE, QUATERNARY describes the conformation assumed by multimeric proteins (aggregates of more than one polypeptide chain).
alpha-Amylases
Monoacylglycerol Lipases
Pinocytosis
Models, Chemical
Temperature
Gram-Negative Anaerobic Straight, Curved, and Helical Rods
Naphthaleneacetic Acids
Enzymes
Endocytosis
Multigene Family
A set of genes descended by duplication and variation from some ancestral gene. Such genes may be clustered together on the same chromosome or dispersed on different chromosomes. Examples of multigene families include those that encode the hemoglobins, immunoglobulins, histocompatibility antigens, actins, tubulins, keratins, collagens, heat shock proteins, salivary glue proteins, chorion proteins, cuticle proteins, yolk proteins, and phaseolins, as well as histones, ribosomal RNA, and transfer RNA genes. The latter three are examples of reiterated genes, where hundreds of identical genes are present in a tandem array. (King & Stanfield, A Dictionary of Genetics, 4th ed)
Electrophoresis, Polyacrylamide Gel
Cell Compartmentation
Mutagenesis, Site-Directed
Vesicular Transport Proteins
A broad category of proteins involved in the formation, transport and dissolution of TRANSPORT VESICLES. They play a role in the intracellular transport of molecules contained within membrane vesicles. Vesicular transport proteins are distinguished from MEMBRANE TRANSPORT PROTEINS, which move molecules across membranes, by the mode in which the molecules are transported.
Aspergillus oryzae
Protein Structure, Secondary
Gangliosidoses
A group of autosomal recessive lysosomal storage disorders marked by the accumulation of GANGLIOSIDES. They are caused by impaired enzymes or defective cofactors required for normal ganglioside degradation in the LYSOSOMES. Gangliosidoses are classified by the specific ganglioside accumulated in the defective degradation pathway.
Xylan Endo-1,3-beta-Xylosidase
alpha-Galactosidase
Golgi Apparatus
A stack of flattened vesicles that functions in posttranslational processing and sorting of proteins, receiving them from the rough ENDOPLASMIC RETICULUM and directing them to secretory vesicles, LYSOSOMES, or the CELL MEMBRANE. The movement of proteins takes place by transfer vesicles that bud off from the rough endoplasmic reticulum or Golgi apparatus and fuse with the Golgi, lysosomes or cell membrane. (From Glick, Glossary of Biochemistry and Molecular Biology, 1990)
Microscopy, Electron
Microscopy using an electron beam, instead of light, to visualize the sample, thereby allowing much greater magnification. The interactions of ELECTRONS with specimens are used to provide information about the fine structure of that specimen. In TRANSMISSION ELECTRON MICROSCOPY the reactions of the electrons that are transmitted through the specimen are imaged. In SCANNING ELECTRON MICROSCOPY an electron beam falls at a non-normal angle on the specimen and the image is derived from the reactions occurring above the plane of the specimen.
Alkaline Phosphatase
Endosomes
Protein Processing, Post-Translational
Any of various enzymatically catalyzed post-translational modifications of PEPTIDES or PROTEINS in the cell of origin. These modifications include carboxylation; HYDROXYLATION; ACETYLATION; PHOSPHORYLATION; METHYLATION; GLYCOSYLATION; ubiquitination; oxidation; proteolysis; and crosslinking and result in changes in molecular weight and electrophoretic motility.
Naphthol AS D Esterase
Biocatalysis
Adenosylhomocysteinase
Liver
Clostridium thermocellum
Neocallimastix
Molecular Structure
Glucan 1,3-beta-Glucosidase
Cathepsin L
Mononuclear Phagocyte System
Mononuclear cells with pronounced phagocytic ability that are distributed extensively in lymphoid and other organs. It includes MACROPHAGES and their precursors; PHAGOCYTES; KUPFFER CELLS; HISTIOCYTES; DENDRITIC CELLS; LANGERHANS CELLS; and MICROGLIA. The term mononuclear phagocyte system has replaced the former reticuloendothelial system, which also included less active phagocytic cells such as fibroblasts and endothelial cells. (From Illustrated Dictionary of Immunology, 2d ed.)
Conserved Sequence
Bacteria
One of the three domains of life (the others being Eukarya and ARCHAEA), also called Eubacteria. They are unicellular prokaryotic microorganisms which generally possess rigid cell walls, multiply by cell division, and exhibit three principal forms: round or coccal, rodlike or bacillary, and spiral or spirochetal. Bacteria can be classified by their response to OXYGEN: aerobic, anaerobic, or facultatively anaerobic; by the mode by which they obtain their energy: chemotrophy (via chemical reaction) or PHOTOTROPHY (via light reaction); for chemotrophs by their source of chemical energy: CHEMOLITHOTROPHY (from inorganic compounds) or chemoorganotrophy (from organic compounds); and by their source for CARBON; NITROGEN; etc.; HETEROTROPHY (from organic sources) or AUTOTROPHY (from CARBON DIOXIDE). They can also be classified by whether or not they stain (based on the structure of their CELL WALLS) with CRYSTAL VIOLET dye: gram-negative or gram-positive.
Carbohydrates
Plants
Multicellular, eukaryotic life forms of kingdom Plantae (sensu lato), comprising the VIRIDIPLANTAE; RHODOPHYTA; and GLAUCOPHYTA; all of which acquired chloroplasts by direct endosymbiosis of CYANOBACTERIA. They are characterized by a mainly photosynthetic mode of nutrition; essentially unlimited growth at localized regions of cell divisions (MERISTEMS); cellulose within cells providing rigidity; the absence of organs of locomotion; absence of nervous and sensory systems; and an alternation of haploid and diploid generations.
Protein Binding
Solubility
Structure-Activity Relationship
Chromatography, Ion Exchange
Centrifugation, Zonal
Dinucleoside Phosphates
Saccharomyces cerevisiae
Fungi
A kingdom of eukaryotic, heterotrophic organisms that live parasitically as saprobes, including MUSHROOMS; YEASTS; smuts, molds, etc. They reproduce either sexually or asexually, and have life cycles that range from simple to complex. Filamentous fungi, commonly known as molds, refer to those that grow as multicellular colonies.
Sequence Homology
Bacillus
Carbohydrate Sequence
Proteins
Linear POLYPEPTIDES that are synthesized on RIBOSOMES and may be further modified, crosslinked, cleaved, or assembled into complex proteins with several subunits. The specific sequence of AMINO ACIDS determines the shape the polypeptide will take, during PROTEIN FOLDING, and the function of the protein.
Muramidase
A basic enzyme that is present in saliva, tears, egg white, and many animal fluids. It functions as an antibacterial agent. The enzyme catalyzes the hydrolysis of 1,4-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrin. EC 3.2.1.17.
Stereoisomerism
O-Acetyl-ADP-Ribose
Centrifugation, Density Gradient
Organophosphorus Compounds
Clostridium
Biological Transport
Penicillin Amidase
Lysosomal Storage Diseases
Arabidopsis
Organelles
Ceroid
Open Reading Frames
Sucrose
Gene Library
Sphingomonas
Interaction of inflammatory cells and oral microorganisms. II. Modulation of rabbit polymorphonuclear leukocyte hydrolase release by polysaccharides in response to Streptococcus mutans and Streptococcus sanguis. (1/2136)
The release of lysosomal hydrolases from polymorphonuclear leukocytes (PMNs) has been postulated in the pathogenesis of tissue injury in periodontal disease. In the present study, lysosomal enzyme release was monitored from rabbit peritoneal exudate PMNs exposed to Streptocccus mutans or Streptococcus sanguis. S. mutans grown in brain heart infusion (BHI) broth failed to promote significant PMN enzyme release. S. sanguis grown in BHI broth, although more effective than S. mutants, was a weak stimulus for promotion of PMN hydrolase release. Preincubation of washed, viable S. mutans in sucrose or in different-molecular-weight dextrans resulted in the ability of the organisms to provoke PMN release reactions. This effect could bot be demonstrated with boiled or trypsinized S. mutans or with viable S. sanguis. However, when grown in BHI broth supplemented with sucrose, but not with glucose, both S. mutans and S. sanguis triggered discharge of PMN enzymes. The mechanism(s) whereby dextran or sucrose modulates PMN-bacterial interaction may in some manner be related to promotion of microbial adhesiveness or aggregation by dextran and by bacterial synthesis of glucans from sucrose. (+info)Interaction of inflammatory cells and oral microorganisms. III. Modulation of rabbit polymorphonuclear leukocyte hydrolase release response to Actinomyces viscosus and Streptococcus mutans by immunoglobulins and complement. (2/2136)
In the absence of antiserum, rabbit polymorphonuclear leukocytes (PMNs) released lysosomal enzymes in response to Actinomyces viscosus (19246) but not to Streptococcus mutans (6715). Antibodies had a marked modulating influence on these reactions. PMN hydrolase release was significantly enhanced to both organisms when specific rabbit antiserum and isolated immunoglobulin G (IgG) were included in the incubations. Immune complex F(ab')2 fragments of IgG directed against S. mutans agglutinated bacteria. Immune complexes consisting of S. mutans and F(ab')2 fragments of IgG directed against this organism were not effective as bacteria-IgG complexes in stimulating PMN release. The intensity of the release response to bacteria-IgG complexes was also diminished when PMNs were preincubated with isolated Fc fragments derived from IgG. Fresh serum as a source of complement components had no demonstrable effect on PMN release either alone or in conjuction with antiserum in these experiments. These data may be relevant to the mechanisms and consequences of the interaction of PMNs and plaque bacteria in the pathogenesis of periodontal disease. (+info)Thermodynamic analysis of halide binding to haloalkane dehalogenase suggests the occurrence of large conformational changes. (3/2136)
Haloalkane dehalogenase (DhlA) hydrolyzes short-chain haloalkanes to produce the corresponding alcohols and halide ions. Release of the halide ion from the active-site cavity can proceed via a two-step and a three-step route, which both contain slow enzyme isomerization steps. Thermodynamic analysis of bromide binding and release showed that the slow unimolecular isomerization steps in the three-step bromide export route have considerably larger transition state enthalpies and entropies than those in the other route. This suggests that the three-step route involves different and perhaps larger conformational changes than the two-step export route. We propose that the three-step halide export route starts with conformational changes that result in a more open configuration of the active site from which the halide ion can readily escape. In addition, we suggest that the two-step route for halide release involves the transfer of the halide ion from the halide-binding site in the cavity to a binding site somewhere at the protein surface, where a so-called collision complex is formed in which the halide ion is only weakly bound. No large structural rearrangements are necessary for this latter process. (+info)A new hydrolase specific for taurine-conjugates of bile acids. (4/2136)
Through the investigation of the bile acid-deconjugation activities of human intestinal anaerobes, a new enzyme was discovered in Peptostreptococcus intermedius which hydrolyzed specifically the taurine-conjugates, but not the glycine-conjugates of bile acids. However, the enzymes in Streptococcus faecalis and Lactobacillus brevis hydrolyzed chiefly the glycine-conjugates. (+info)Identification and characterization of alkenyl hydrolase (lysoplasmalogenase) in microsomes and identification of a plasmalogen-active phospholipase A2 in cytosol of small intestinal epithelium. (5/2136)
A lysoplasmalogenase (EC 3.3.2.2; EC 3.3.2.5) that liberates free aldehyde from 1-alk-1'-enyl-sn-glycero-3-phospho-ethanolamine or -choline (lysoplasmalogen) was identified and characterized in rat gastrointestinal tract epithelial cells. Glycerophosphoethanolamine was produced in the reaction in equimolar amounts with the free aldehyde. The microsomal membrane associated enzyme was present throughout the length of the small intestines, with the highest activity in the jejunum and proximal ileum. The rate of alkenyl ether bond hydrolysis was dependent on the concentrations of microsomal protein and substrate, and was linear with respect to time. The enzyme hydrolyzed both ethanolamine- and choline-lysoplasmalogens with similar affinities; the Km values were 40 and 66 microM, respectively. The enzyme had no activity with 1-alk-1'-enyl-2-acyl-sn-glycero-3-phospho-ethanolamine or -choline (intact plasmalogen), thus indicating enzyme specificity for a free hydroxyl group at the sn-2 position. The specific activities were 70 nmol/min/mg protein and 57 nmol/min/mg protein, respectively, for ethanolamine- and choline-lysoplasmalogen. The pH optimum was between 6.8 and 7.4. The enzyme required no known cofactors and was not affected by low mM levels of Ca2+, Mg2+, EDTA, or EGTA. The detergents, Triton X-100, deoxycholate, and octyl glucoside inhibited the enzyme. The chemical and physical properties of the lysoplasmalogenase were very similar to those of the enzyme in liver and brain microsomes. In developmental studies the specific activities of the small intestinal and liver enzymes increased markedly, 11.1- and 3.4-fold, respectively, in the first approximately 40 days of postnatal life. A plasmalogen-active phospholipase A2 activity was identified in the cytosol of the small intestines (3.3 nmol/min/mg protein) and liver (0.3 nmol/min/mg protein) using a novel coupled enzyme assay with microsomal lysoplasmalogenase as the coupling enzyme. (+info)Galactosyltransferase, pyrophosphatase and phosphatase activities in luminal plasma of the cauda epididymidis and in the rete testis fluid of some mammals. (6/2136)
Galactosyltransferase activity was measured in the luminal plasma of the cauda epididymidis of mice, rats, rabbits, rams and boars, and in the rete testis fluid of rams and boars. The activities of nucleotide pyrophosphatase and alkaline phosphatase, which compete with galactosyltransferase for substrate, were also determined. In these species, galactosyltransferase activity in the luminal plasma of the cauda epididymidis was similar when the inhibitory effect of pyrophosphatase and phosphatase was minimized by assay conditions. However, under assay conditions that did not minimize the effect of these enzymes, the galactosyltransferase activities of these species were very different and were inversely correlated with the activities of pyrophosphatase and phosphatase. The ratio of galactosyltransferase activity to pyrophosphatase and phosphatase activity was much higher in the rete testis fluid than in the luminal plasma of the cauda epididymidis in both rams and boars. In rams, galactosyltransferase in the luminal plasma of the cauda epididymidis was more heat resistant than that in serum. These results suggest that there is a species difference in the availability of galactosyltransferase activity in the luminal plasma of the cauda epididymidis and that in some species, galactosyltransferase in the luminal fluid is unlikely to have any function. The results are also discussed with respect to the possible function of galactosyltransferase, pyrophosphatase and phosphatase in epididymal luminal plasma and rete testis fluid. (+info)Molecular cloning of cDNAs of mouse peptidylarginine deiminase type I, type III and type IV, and the expression pattern of type I in mouse. (7/2136)
Peptidylarginine deiminases (PADs), a group of post-translational enzymes, catalyze the conversion of protein-bound arginine residues to citrulline residues in a calcium ion-dependent manner and are widely distributed in various organs of vertebrates. Although the existence of four isoforms of PAD (types I, II, III, and IV) is reported in rodents, the relative functions of the isoforms with respect to their colocation in the tissues have yet to be explored. In this study, we cloned the full-length cDNA encoding mouse PAD type I by screening a uterine cDNA library and using the RACE method. This cDNA consists of an open reading frame of 1989 bases encoding 662 amino acids (73,823 Da), a 5'-untranslated region of 127 bases and a 3'-untranslated region of 1639 bases. Comparative reverse transcription-PCR and Northern-blot analyses detected PAD type I mRNA only in the epidermis and uterus. Administration of estrogen to adult ovariectomized mice increased the content of PAD type I mRNA in the uterus, providing evidence that its expression is under the control of the sex steroid hormone. We also cloned the full-length cDNAs of mouse PAD type III and type IV by the reverse transcription-PCR and RACE methods. The primary structure of PAD type III contains 664 amino acids (75,098 Da) deduced from the coding region of 1995 bases, and the primary structure of PAD type IV consists of 666 amino acids (74,475 Da) deduced from the coding region of 2001 bases. Comparison of the deduced amino acid sequences of all four isoforms of PAD showed about 50% identity with each other, the 3' regions being highly homologous compared with the 5' regions. (+info)Degradation of 1,2-dibromoethane by Mycobacterium sp. strain GP1. (8/2136)
The newly isolated bacterial strain GP1 can utilize 1, 2-dibromoethane as the sole carbon and energy source. On the basis of 16S rRNA gene sequence analysis, the organism was identified as a member of the subgroup which contains the fast-growing mycobacteria. The first step in 1,2-dibromoethane metabolism is catalyzed by a hydrolytic haloalkane dehalogenase. The resulting 2-bromoethanol is rapidly converted to ethylene oxide by a haloalcohol dehalogenase, in this way preventing the accumulation of 2-bromoethanol and 2-bromoacetaldehyde as toxic intermediates. Ethylene oxide can serve as a growth substrate for strain GP1, but the pathway(s) by which it is further metabolized is still unclear. Strain GP1 can also utilize 1-chloropropane, 1-bromopropane, 2-bromoethanol, and 2-chloroethanol as growth substrates. 2-Chloroethanol and 2-bromoethanol are metabolized via ethylene oxide, which for both haloalcohols is a novel way to remove the halide without going through the corresponding acetaldehyde intermediate. The haloalkane dehalogenase gene was cloned and sequenced. The dehalogenase (DhaAf) encoded by this gene is identical to the haloalkane dehalogenase (DhaA) of Rhodococcus rhodochrous NCIMB 13064, except for three amino acid substitutions and a 14-amino-acid extension at the C terminus. Alignments of the complete dehalogenase gene region of strain GP1 with DNA sequences in different databases showed that a large part of a dhaA gene region, which is also present in R. rhodochrous NCIMB 13064, was fused to a fragment of a haloalcohol dehalogenase gene that was identical to the last 42 nucleotides of the hheB gene found in Corynebacterium sp. strain N-1074. (+info)
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PADI4 - Wikipedia
Citrullination: a posttranslational modification in health and disease
Enzyme deregulation in disease
Regulating Transcription Through Arginine Deimination | Science Signaling
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Cit17]-Histone H3 (1-21)-GGK(Biotin)
Protein that switches cancers from inflammation to proliferation identified. ecancer - News
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OCA:4N24
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IAA-amino acid hydrolase elisa and antibody
Gel Pad Activity Center | Enabling Devices
TIGR02252
Biology-Online • View topic - Hydrolases
RCSB PDB - Protein Feature View
- Haloalkane dehalogenase 3 - P9WMR9 (DHAA MYCTU)
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RCSB PDB - Protein Feature View
- Haloacid dehalogenase, type II - Q12G50 (Q12G50 POLSJ)
BIOETYMOLOGY : ORIGIN IN BIOMEDICAL TERMS: hydrolase
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Peptidylarginine deiminase 2 is required for tumor necrosis factor alpha-induced citrullination and arthritis, but not...
Fumarylacetoacetate hydrolase - Wikipedia
atrazine chlorohydrolase
Summary Report | CureHunter
Iguratimod dose dependently inhibits the expression of citrullinated proteins and peptidylarginine deiminases 2 and 4 in...
Arginine deiminase augments the chemosensitivity of argininosuccinate synthetase-deficient pancreatic cancer cells to...
Some Biochemical Properties and the Classification of a Range of Bacterial Haloalkane Dehalogenases | Protein Engineering Group
The Devil in the Details: The Emerging Role of Anticitrulline Autoimmunity in Rheumatoid Arthritis | The Journal of Immunology
A C-terminal phosphatase module conserved in vertebrate CMP-sialic acid synthetases provides a tetramerization interface for...
Citrullinated proteins have increased immunogenicity and arthritogenicity and their presence in arthritic joints correlates...
Spontaneous secretion of peptidyl-arginine deiminase 2 and cell surface exposure of peptidyl-arginine deiminase 4 by...
Mechanistic studies of agmatine deiminase from multiple bacterial spec by Justin E. Jones, Christina J. Dreyton et al.
Depletion of Arginine by Recombinant Arginine Deiminase Induces nNOS-Activated Neurotoxicity in Neuroblastoma Cells
Spinal cord regeneration: A role for peptidylarginine deiminase (PAD) in secondary injury response : WestminsterResearch
Alpha/beta hydrolase domain-containing protein 3 | definition of alpha/beta hydrolase domain-containing protein 3 by Medical...
Abstract 20419: Citrullination in Actin-Tropomyosin-Myosin Complex: Novel Regulatory Mechanism | Circulation
Expression of citrulline and homocitrulline residues in the lungs of non-smokers and smokers: implications for autoimmunity in...
Dehalogenase | definition of dehalogenase by Medical dictionary
Electron-Transfer Photoredox Catalysis: Development of a Tin-Free Reductive Dehalogenation Reaction
Deuterium Exchange Measurement | Profiles RNS
Plus it
In silico design of potentially functional artificial metallo-haloalkane dehalogenase containing catalytic zinc. | FoldX
Hydrocarbon Dehalogenation in a Tortuous Microreactor
Citrullination
Proteomic characterization of serine hydrolase activity and composition in normal urine
DMOZ - Health: Conditions and Diseases: Nutritional and Metabolic Disorders: Inherited: Tyrosinemia
Objective The mechanisms that donate to the persistent activation of macrophages - Novel therapeutic agents in the treatment of...
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Citrullination regulates pluripotency and histone H1 binding to chromatin.
CATH Superfamily 3.40.50.1820
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Arginine - Nitric Oxide - L-Arginine - Cardiovascular - CardioMAXX
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Sequence Similarity Search - BLAST
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The entrance to the central cavity of the AtzA hexamer | Open-i
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Tree of glycosyl hydrolases of the GH13 family. Full ge | Open-i
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Assay Procedure for Creatinine Deiminase Microbial | China-Mainland | Sigma-Aldrich
PADI4 polyclonal antibody - (PAB14053) - Products - Abnova
Maltase-glucoamylase
"Glycoside hydrolases". CAZypedia. Retrieved 2021-04-30. Frandsen TP, Svensson B (May 1998). "Plant alpha-glucosidases of the ... glycoside hydrolase family 31. Molecular properties, substrate specificity, reaction mechanism, and comparison with family ...
Levanase
... (EC 3.2.1.65, levan hydrolase, 2,6-beta-D-fructan fructanohydrolase) is an enzyme with systematic name (2->6)-beta-D- ... "Fructan hydrolases". Methods Enzymol. 8: 621-628. doi:10.1016/0076-6879(66)08112-6. Levanase at the US National Library of ...
GTP cyclohydrolase II
This enzyme belongs to the family of hydrolases, those acting on carbon-nitrogen bonds other than peptide bonds, specifically ...
Succinyl-diaminopimelate desuccinylase
This enzyme belongs to the family of hydrolases, those acting on carbon-nitrogen bonds other than peptide bonds, specifically ...
CDC42
Its sequence domains include a P-loop containing nucleoside triphosphate hydrolase and a small GTP-binding protein domain.[6] ...
G protein
EC3 Hydrolases (list). *EC4 Lyases (list). *EC5 Isomerases (list). *EC6 Ligases (list) ...
GTPase
... s are a large family of hydrolase enzymes that bind to the nucleotide guanosine triphosphate (GTP) and hydrolyze it to ...
Dynein
Hydrolases: acid anhydride hydrolases (EC 3.6). 3.6.1. *Pyrophosphatase *Inorganic. *Thiamine. *Apyrase ...
Kynureninase
hydrolase activity. • 3-hydroxykynureninase activity. Cellular component. • cytosol. • mitochondrion. • cytoplasm. • ... Kynureninase or L-Kynurenine hydrolase (KYNU) (EC 3.7.1.3) is a PLP dependent enzyme that catalyses the cleavage of kynurenine ...
HDAC4
hydrolase activity. • protein deacetylase activity. • identical protein binding. • core promoter binding. • RNA polymerase II ...
Baway Wala
Mier, P. D.; van den Hurk, J. J. (October 1975). "Lysosomal hydrolases of the epidermis. 2. Ester hydrolases". The British ...
Catalytic triad
Cysteine and serine hydrolasesEdit. The same triad geometries been converged upon by serine proteases such as the chymotrypsin[ ... The same triad has also convergently evolved in α/β hydrolases such as some lipases and esterases, however orientation of the ... Enzymes that contain a catalytic triad use it for one of two reaction types: either to split a substrate (hydrolases) or to ... 2017). "Alpha/beta-hydrolases: A unique structural motif coordinates catalytic acid residue in 40 protein fold families". ...
Granulocyte
Acid hydrolases: further digest bacteria. *Lysozyme: dissolves cell walls of some gram-positive bacteria ...
X8 protein domain
The domain is also found in an Olive pollen allergen as well as at the C terminus of family 17 glycosyl hydrolases. Simpson C, ... Henrissat B, Davies GJ (December 2000). "Glycoside hydrolases and glycosyltransferases. Families, modules, and implications for ...
Purine nucleosidase
... ribonucleoside hydrolase, nucleoside hydrolase, N-ribosyl purine ribohydrolase, nucleosidase g, N-D-ribosylpurine ribohydrolase ... This enzyme belongs to the family of hydrolases, specifically those glycosylases that hydrolyse N-glycosyl compounds. The ... Tarr HLA (1955). "Fish muscle riboside hydrolases". Biochem. J. 59 (3): 386-391. doi:10.1042/bj0590386. PMC 1216255. PMID ... Mazumder-Shivakumar D, Bruice TC (2005). "Computational study of IAG-nucleoside hydrolase: determination of the preferred ...
Oligosaccharyltransferase
EC3 Hydrolases (list). *EC4 Lyases (list). *EC5 Isomerases (list). *EC6 Ligases (list) ...
Beta-alanine-pyruvate transaminase
EC3 Hydrolases (list). *EC4 Lyases (list). *EC5 Isomerases (list). *EC6 Ligases (list) ...
2-alkenal reductase
EC3 Hydrolases (list). *EC4 Lyases (list). *EC5 Isomerases (list). *EC6 Ligases (list) ...
Glycogen phosphorylase
EC3 Hydrolases (list). *EC4 Lyases (list). *EC5 Isomerases (list). *EC6 Ligases (list) ...
Superoxide dismutase
EC3 Hydrolases (list). *EC4 Lyases (list). *EC5 Isomerases (list). *EC6 Ligases (list) ...
L-xylulose reductase
EC3 Hydrolases (list). *EC4 Lyases (list). *EC5 Isomerases (list). *EC6 Ligases (list) ...
Cyclin-dependent kinase 4
EC3 Hydrolases (list). *EC4 Lyases (list). *EC5 Isomerases (list). *EC6 Ligases (list) ...
Beta-glucosidase
Hydrolase: sugar hydrolases (EC 3.2). 3.2.1: Glycoside hydrolases. Disaccharidase. *Sucrase/Sucrase-isomaltase/Invertase ...
Homoisocitrate dehydrogenase
EC3 Hydrolases (list). *EC4 Lyases (list). *EC5 Isomerases (list). *EC6 Ligases (list) ...
Aminoacyl tRNA synthetase
EC3 Hydrolases (list). *EC4 Lyases (list). *EC5 Isomerases (list). *EC6 Ligases (list) ...
Phosphogluconate dehydratase
EC3 Hydrolases (list). *EC4 Lyases (list). *EC5 Isomerases (list). *EC6 Ligases (list) ...
Factor X
hydrolase activity. Cellular component. • endoplasmic reticulum lumen. • intrinsic component of external side of plasma ...
Glutamate decarboxylase
EC3 Hydrolases (list). *EC4 Lyases (list). *EC5 Isomerases (list). *EC6 Ligases (list) ...
Muramyl ligase
EC3 Hydrolases (list). *EC4 Lyases (list). *EC5 Isomerases (list). *EC6 Ligases (list) ...
Complement component 1s
hydrolase activity. • metal ion binding. • identical protein binding. • serine-type endopeptidase activity. ...
Category:Hydrolases - Wikimedia Commons
hidrolasa (es); 加水分解酵素 (yue); Hidrolasa (eu); Гидролазы (ru); Hydrolase (de-ch); Hydrolase (de); hydrolase (en-gb); Հիդրոլազներ ... hydrolase (sco); Гідролази (uk); hydrolase (nn); hydrolase (nl); Hidrolaza (sh); 水解酶 (zh); hydrolazy (pl); Hydrolaasit (fi); ... Hydrolase (en-ca); hydroláza (cs); நீராற்பகுப்பி (ta); Idrolasi (it); hydrolase (fr); Гідралязы (be-tarask); hidrolasa (ca); ... Media in category "Hydrolases". The following 175 files are in this category, out of 175 total. ...
Glycoside hydrolase - Wikipedia
Glycoside hydrolases (also called glycosidases or glycosyl hydrolases) catalyze the hydrolysis of glycosidic bonds in complex ... Main article: List of glycoside hydrolase families. Glycoside hydrolases are classified into EC 3.2.1 as enzymes catalyzing the ... Bairoch, A. "Classification of glycosyl hydrolase families and index of glycosyl hydrolase entries in SWISS-PROT". 1999. ... a b CAZy Family Glycoside Hydrolase *^ Henrissat B, Callebaut I, Mornon JP, Fabrega S, Lehn P, Davies G (1995). "Conserved ...
Leukotriene-A4 hydrolase - Wikipedia
14-tetraenoate hydrolase. Other names in common use include LTA4 hydrolase, LTA4H, and leukotriene A4 hydrolase. This enzyme ... This enzyme belongs to the family of hydrolases, specifically those acting on ether bonds (ether hydrolases). The systematic ... Leukotriene A4 hydrolase, also known as LTA4H is a human gene.[1][2][3] The protein encoded by this gene is a bifunctional ... This hydrolase article is a stub. You can help Wikipedia by expanding it.. *v ...
Phosphoric monoester hydrolases - Wikipedia
Acid anhydride hydrolases - Wikipedia
Acid anhydride hydrolases are a class of hydrolase enzymes that catalyze the hydrolysis of an acid anhydride bond. They are ... 2012). Acid Anhydride Hydrolases: Advances in Research and Application. Media related to Acid anhydride hydrolases at Wikimedia ... Commons Acid+anhydride+hydrolases at the US National Library of Medicine Medical Subject Headings (MeSH) Biology portal v t e. ...
Bacterial peptidoglycan (murein) hydrolases
An overview of the different classes of peptidoglycan hydrolases and their cleavage sites is provided. The physiological ... Most bacteria have multiple peptidoglycan hydrolases capable of cleaving covalent bonds in peptidoglycan sacculi or its ... Bacterial peptidoglycan (murein) hydrolases FEMS Microbiol Rev. 2008 Mar;32(2):259-86. doi: 10.1111/j.1574-6976.2007.00099.x. ... Specialized hydrolases enlarge the pores in the peptidoglycan for the assembly of large trans-envelope complexes (pili, ...
Organic Solvent Tolerance of Retro-Friedel-Crafts Hydrolases | SpringerLink
These unique hydrolases are scarcely studied for biocatalytical applications in organic... ... Retro-Friedel-Crafts hydrolases are co-factor independent enzymes with unusual reactivity and selectivity. ... Two Friedel-Crafts hydrolases were selected, namely 2,6-diacetylphloroglucinol hydrolase (PhlG) from Pseudomonas fluorescens ... C-C hydrolase Enzyme stability Solvent tolerance Retro-Friedel-Crafts acylation This is a preview of subscription content, log ...
Production of polysaccharide hydrolases in the genus Rhizopus | SpringerLink
Polysaccharide hydrolase activity was assayed in a group of 28 selected Rhizopusstrains. The production of lichenases, ... Polysaccharide hydrolase activity was assayed in a group of 28 selectedRhizopus strains. The production of lichenases, ... Lukášová J., Zemek J., Augustín J., Kuniak L.: Production of hydrolases in staphylococci isolated from foodstuffs.Arch. ... Vincúrová M., Zemek J., Augustín J., Schwartz E., Kuniak L.: Hydrolases of polysaccharides in typical and atypical ...
Glycoside hydrolase superfamily (IPR017853) | InterPro | EMBL-EBI
Glycoside hydrolase superfamily (IPR017853). Short name: Glycoside_hydrolase_SF Overlapping entries *Glycoside hydrolase family ... Glycosyl hydrolases family 1, N-terminal conserved site (IPR033132). *Glycosyl hydrolase family 30, TIM-barrel domain ( ... Glycoside hydrolase, family 2, active site (IPR023232). *Uncharacterised protein family, glycosyl hydrolase catalytic domain ( ... O-Glycosyl hydrolases (EC:3.2.1.) are a widespread group of enzymes that hydrolyse the glycosidic bond between two or more ...
RCSB PDB - 1RGE: HYDROLASE, GUANYLORIBONUCLEASE
As a member of the wwPDB, the RCSB PDB curates and annotates PDB data according to agreed upon standards. The RCSB PDB also provides a variety of tools and resources. Users can perform simple and advanced searches based on annotations relating to sequence, structure and function. These molecules are visualized, downloaded, and analyzed by users who range from students to specialized scientists.
Microbial glycoside hydrolases as antibiofilm agents with cross-kingdom activity | PNAS
Recombinant Hydrolase Expression and Purification.. Hydrolases were expressed and purified as described (22, 23). ... Quantification of Hydrolase Binding to Culture Supernatants.. Details of culture supernatant production and hydrolase binding ... Treatment of A. fumigatus with Glycoside Hydrolases.. To visualize the effects of hydrolases on cell wall-associated GAG, ... Recombinant hydrolase domains from these proteins (Sph3h from A. fumigatus and PelAh from P. aeruginosa) were found to degrade ...
Glycoside hydrolase, family 45 (IPR000334) | InterPro | EMBL-EBI
Glycoside hydrolase, family 45 (IPR000334). Short name: Glyco_hydro_45 Domain relationships *RlpA-like protein, double-psi beta ... O-Glycosyl hydrolases (EC:3.2.1.) are a widespread group of enzymes that hydrolyse the glycosidic bond between two or more ... Glycoside hydrolase family 45 GH45 comprises enzymes with only one known activity; endoglucanase (EC:3.2.1.4). ... One of these families is known as the cellulase family K or as the glycosyl hydrolases family 45 [PMID: 8352747]. The best ...
MULTISPECIES: glycosyl hydrolase [Bacteroides] - Protein - NCBI
Lysosomal hydrolases of the epidermis. I. Glycosidases
Pla2g5 hydrolase gene ontology
Fig4 hydrolase gene ontology
Glycoside hydrolases and glycosyltransferases: families and functional modules. - PubMed - NCBI
Glycoside hydrolases and glycosyltransferases: families and functional modules.. Bourne Y1, Henrissat B. ... The past year has witnessed the expected increase in the number of solved structures of glycoside hydrolases and ... These structures show that, while glycoside hydrolases display an extraordinary variety of folds, glycosyltransferases and ...
Industrial Example 1: Hydrolases - Pharmaceuticals and Fine Chemicals | Coursera
In this example, were going to look at the industrial use of hydrolase enzymes, in particular the use of hydrolases to ... Industrial Example 1: Hydrolases. To view this video please enable JavaScript, and consider upgrading to a web browser that ... Lipase is a hydrolase and in this case its working in reverse. So rather than hydrolyzing an ester were converting an alcohol ... Many hydrolase reactions are enantioselective and the enantioselectivity is governed by the chiral nature of protein. The ...
RCSB PDB - 1GQ6: PROCLAVAMINATE AMIDINO HYDROLASE FROM STREPTOMYCES CLAVULIGERUS
PROCLAVAMINATE AMIDINO HYDROLASE. A, B, C. 313. Streptomyces clavuligerus. Mutation(s): 0 Gene Names: pah. EC: 3.5.3.11 (PDB ... Oligomeric Structure of Proclavaminic Acid Amidino Hydrolase: Evolution of a Hydrolytic Enzyme in Clavulanic Acid Biosynthesis ... by clavaminic acid synthase is separated from the other two by a step catalysed by proclavaminic acid amidino hydrolase (PAH), ... by clavaminic acid synthase is separated from the other two by a step catalysed by proclavaminic acid amidino hydrolase (PAH), ...
Patent US20020061580 - Alpha/beta hydrolase-fold enzymes - Google Patents
The present invention provides amino acid sequences for the hydrolase. The present invention provides host cells which comprise ... animal feeds and compositions used to treat a textile that include the hydrolase of the present invention. ... The present invention relates to the identification of novel hydrolases in gram positive microorganisms. ... nucleic acid encoding the hydrolase. The present invention also provides cleaning compositions, ...
Serine hydrolase
... The serine hydrolase superfamily is one of the largest known enzyme families comprising approximately 1% of ... Hydrolase: esterases (EC 3.1). 3.1.1: Carboxylic ester hydrolases. Cholinesterase - Pectinesterase - 6-phosphogluconolactonase ... EC1 Oxidoreductases/list - EC2 Transferases/list - EC3 Hydrolases/list - EC4 Lyases/list - EC5 Isomerases/list - EC6 Ligases/ ... The serine hydrolase superfamily is one of the largest known enzyme families comprising approximately 1% of the genes in the ...
carboxylic ester hydrolase activity | SGD
Gene Ontology Term: carboxylic ester hydrolase activity. GO ID. GO:0052689 Aspect. Molecular Function. Description. Catalysis ... carboxylic ester hydrolase activity, carboxylic esterase activity, cocaine esterase activity, esterase A, esterase B, ... carboxyl ester hydrolase activity, carboxylate esterase activity, carboxylesterase activity, carboxylic acid esterase activity ...
EPO - T 0823/12 (Glycosyl hydrolase/NOVOZYMES) of 23.8.2016
Glycosyl hydrolase family 61 (GH61) was one of 60 different families of glycosyl hydrolases (D9), and not all its members - ... T 0823/12 (Glycosyl hydrolase/NOVOZYMES) of 23.8.2016. European Case Law Identifier:. ECLI:EP:BA:2016:T082312.20160823. ... 2.8 The appellant further argued that, even if starting from D1 as the closest prior art, it was known that glycosyl hydrolases ... Even if one expected that more glycosyl hydrolase genes could be found, these would not necessarily be members of the GH61 ...
NIOSHTIC-2 Publications Search - 00036312 - In vitro hydrolase and phagocytic activities of alveolar macrophages.
N-carbamoyl-D-amino acid hydrolase - Ensifer adhaerens
CN hydrolasePROSITE-ProRule annotation. ,p>Manual validated information which has been generated by the UniProtKB automatic ... N-carbamoyl-D-amino acid hydrolase activity Source: UniProtKB ,p>Inferred from Direct Assay,/p> ,p>Used to indicate a direct ... N-carbamoyl-D-amino acid hydrolase1 Publication. ,p>Manually curated information which has been inferred by a curator based on ... sp,Q5S260,DCAS_ENSAD N-carbamoyl-D-amino acid hydrolase OS=Ensifer adhaerens OX=106592 PE=1 SV=1 ...
Site Directed Mutagenesis Of Dienelactone Hydrolase - Digital Library
Enzyme assays showed that dienelactone hydrolase activity of the mutants Arg 81 and Arg 206 was totally abolished. The DLHase ... Site-specific mutants of dienelactone hydrolase were created using mismatched oligonucleotides to prime DNA synthesis. ... The role of individual amino acid residues of the enzyme dienelactone hydrolase was investigated. Using the polymerase chain ... Chen, Wei, 1965-. Site Directed Mutagenesis Of Dienelactone Hydrolase, thesis, December 1992; Denton, Texas. (digital.library. ...
Peptide hydrolases | definition of Peptide hydrolases by Medical dictionary
What is Peptide hydrolases? Meaning of Peptide hydrolases medical term. What does Peptide hydrolases mean? ... Looking for online definition of Peptide hydrolases in the Medical Dictionary? Peptide hydrolases explanation free. ... redirected from Peptide hydrolases). Also found in: Dictionary, Thesaurus, Encyclopedia. endopeptidase. [en″do-pep´tĭ-dās] any ... Peptide hydrolases , definition of Peptide hydrolases by Medical dictionary https://medical-dictionary.thefreedictionary.com/ ...
Anti-Epoxide hydrolase antibody (ab96695) | Abcam
Rabbit polyclonal Epoxide hydrolase antibody validated for WB, IHC, ICC/IF and tested in Human. Referenced in 1 publication. ... All lanes : Anti-Epoxide hydrolase antibody (ab96695) at 1/1000 dilution. Lane 1 : HeLa whole cell lysate. Lane 2 : HepG2 whole ... ab96695, at a 1/200 dilution, staining Epoxide hydrolase in methanol fixed HeLa cells by Immunofluorescence analysis. Lower ... ab96695, at a 1/500 dilution, staining Epoxide hydrolase in paraffin embedded SG xenograft by Immunohistochemical analysis. ...
Anti-LTA4 Hydrolase Antibody Products | Biocompare
Compare Anti-LTA4 Hydrolase Antibody Products from leading suppliers on Biocompare. View specifications, prices, citations, ... Anti-LTA4 Hydrolase Antibody Products. Listed below are anti-LTA4 Hydrolase antibodies from multiple suppliers. LTA4 Hydrolase ... is a reported alias name for the human gene LTA4H, or leukotriene A4 hydrolase. The 611-amino acid protein is a member of the ...
Gene-centric metagenomics of the fiber-adherent bovine rumen microbiome reveals forage specific glycoside hydrolases | PNAS
2005) Novel hydrolase diversity retrieved from a metagenome library of bovine rumen microflora. Environ Microbiol 7:1996-2010. ... Other abundant glycosyl hydrolase families, which contain enzymes active on side chains of hemicelluloses and pectins, include ... Gene-centric metagenomics of the fiber-adherent bovine rumen microbiome reveals forage specific glycoside hydrolases. Jennifer ... In contrast, metagenomic analysis of a bovine rumen expression library (17) identified 22 glycoside hydrolase (GH) clones of ...
EnzymeGlycoside hydrolaseCell Wall HydrolaseLeukotrieneInhibitionProteinInhibitorsFAAHGeneFattyFumarylacetoacetate hydrolasePeptideHydrolyticHydrolysisOrganismEster hydrolase activityCatalyticSuperfamilyCatalyzeAntibodiesActivityDegradationEpoxide hydrolasesGlycosyl hydrolasesBacterial hydrolasesPeptidoglycan hydrolasesBacteriaMicrosomalEPHX2SubstratesLysosomalMetabolismClassificationArabidopsisEpoxyeicosatrienoic acidsIntestinalAcid anhydrideAminoReactivityOrganophosphorusNucleicPolysaccharide
Enzyme17
- One of the important occurrences of glycoside hydrolases in bacteria is the enzyme beta-galactosidase (LacZ), which is involved in regulation of expression of the lac operon in E. coli . (wikipedia.org)
- This enzyme belongs to the family of hydrolases , specifically those acting on ether bonds (ether hydrolases). (wikipedia.org)
- The serine hydrolase superfamily is one of the largest known enzyme families comprising approximately 1% of the genes in the human genome. (bionity.com)
- The role of individual amino acid residues of the enzyme dienelactone hydrolase was investigated. (unt.edu)
- Enzyme assays showed that dienelactone hydrolase activity of the mutants Arg 81 and Arg 206 was totally abolished. (unt.edu)
- Objectives: The purpose of this study was to determine if polymorphisms in microsomal epoxide hydrolase, an enzyme involved in the metabolism of reactive intermediates of vinyl chloride (VC), contribute to the variable susceptibility to the mutagenic effects of vinyl chloride among exposed workers. (cdc.gov)
- In biochemistry , a hydrolase is an enzyme that catalyzes the hydrolysis of a chemical bond . (wikidoc.org)
- The latter pathway features an isothiocyanate hydrolase that is homologous to a previously characterized bacterial enzyme, and converts isothiocyanate into products that are not toxic to the fungus. (nature.com)
- These endogenous lipid mediators are converted into inactive diols by soluble epoxide hydrolase (sEH), and so inhibiting this enzyme would be expected to enhance the stability and therapeutic actions of EETs [ 17 ]. (hindawi.com)
- Thus, human tumors can metabolize BLM, and while BLM hydrolase activity may be important in tumor resistance to the drug, these data suggest that either ( a ) the enzyme activity in the tumor homogenate does not reflect that in the clonogenic cells or ( b ) other mechanisms of resistance may be operative. (aacrjournals.org)
- The specific activities of three glycosyl hydrolases, including an α-glucosidase ( malA ), a β-glycosidase ( lacS ), and the major secreted α-amylase, were measured in the mutant strains using enzyme activity assays, Western blot analysis, and Northern blot analysis. (genetics.org)
- These revertants were pleiotropic and restored glycosyl hydrolase activity either partially or completely to wild-type levels as indicated by enzyme assays and Western blots. (genetics.org)
- Catalytic characteristics of hexahistidine-containing organophosphorus hydrolase (His 6 -OPH) and its enzyme-polyelectrolyte complexes with poly- l -glutamic acid or poly- l -aspartic acid (His 6 -OPH/PLD 50 ), hydrolyzing organophosphorous compounds, and N -acyl homoserine lactones were studied in the presence of various antibiotics (ampicillin, gentamicin, kanamycin, and rifampicin). (mdpi.com)
- A key enzyme in chronic lung inflammation is leukotriene A4 hydrolase (LTA4H). (harvard.edu)
- The Purification and Characterization of Phosphonopyruvate Hydrolase, a Novel Carbon-Phosphorus Bond Cleavage Enzyme from Variovorax sp. (semanticscholar.org)
- Acyl-enzyme intermediates are formed during lipase-catalyzed reactions and in an analogous way, retaining glycoside hydrolases form glycosyl-enzyme intermediates during catalysis. (lu.se)
- Probing enzyme promiscuity of SGNH hydrolases. (semanticscholar.org)
Glycoside hydrolase3
- A comparison of the glycoside hydrolase and cellulosome functional genes revealed that in the rumen microbiome, initial colonization of fiber appears to be by organisms possessing enzymes that attack the easily available side chains of complex plant polysaccharides and not the more recalcitrant main chains, especially cellulose. (pnas.org)
- The glycoside hydrolase family 3 (GH3) β -glucosidases are a structurally diverse family of enzymes. (iucr.org)
- Glycoside hydrolase family 3 (GH3) is one of the larger families in the CAZy classification and currently contains over 13 600 annotated protein sequences. (iucr.org)
Cell Wall Hydrolase3
- Effect of wounding on cell wall hydrolase activity in tomato fruit. (springer.com)
- Bacterial SH3 (SH3b) domains are commonly fused with papain-like Nlp/P60 cell wall hydrolase domains. (osti.gov)
- Each cell wall hydrolase (CWH) has a specific role regarding the PG: (i) cell wall amidase (CWA) cleaves the amide bond between N-acetylmuramic acid and L-alanine residue at the N-terminal of the stem peptide, (ii) cell wall glycosidase (CWG) catalyses the hydrolysis of the glycosidic linkages, whereas (iii) cell wall peptidase (CWP) cleaves amide bonds between amino acids within the PG chain. (frontiersin.org)
Leukotriene11
- Leukotriene A4 hydrolase , also known as LTA4H is a human gene . (wikipedia.org)
- Other names in common use include LTA4 hydrolase , LTA4H , and leukotriene A4 hydrolase . (wikipedia.org)
- Molecular cloning of a cDNA coding for human leukotriene A4 hydrolase. (wikipedia.org)
- Leukotriene A4 hydrolase in the human lung. (wikipedia.org)
- Other enzymes with epoxide hydrolase activity include leukotriene A4 hydrolase , Cholesterol-5,6-oxide hydrolase , MEST (gene) (Peg1/MEST), and Hepoxilin-epoxide hydrolase . (wikidoc.org)
- Epoxide hydrolase that catalyzes the final step in the biosynthesis of the proinflammatory mediator leukotriene B4. (antibodies-online.com)
- Additionally we are shipping Leukotriene A4 Hydrolase Antibodies (94) and Leukotriene A4 Hydrolase Proteins (14) and many more products for this protein. (antibodies-online.com)
- Mariana Trivilin Mendes and Paulo Flavio Silveira, "The Interrelationship between Leukotriene B4 and Leukotriene-A4-Hydrolase in Collagen/Adjuvant-Induced Arthritis in Rats," BioMed Research International , vol. 2014, Article ID 730421, 9 pages, 2014. (hindawi.com)
- The leukotriene A 4 hydrolase (LTA 4 H) protein is regarded as a relevant target for cancer therapy. (aacrjournals.org)
- Characterization of human airway epithelial cell leukotriene A4 hydrolase. (ovid.com)
- We have previously shown that human airway epithelial cells contain leukotriene A4 (LTA4) hydrolase activity. (ovid.com)
Inhibition3
- investigated whether inhibition of fatty acid amide hydrolase (FAAH) alters responses of dorsal horn neurons in neuropathic pain ( Fig. 1 ). (jneurosci.org)
- Epoxyeicosatrienoic and dihydroxyeicosatrienoic acids dilate human coronary arterioles via BK(Ca) channels: implications for soluble epoxide hydrolase inhibition. (wikipathways.org)
- Soluble epoxide hydrolase inhibition reveals novel biological functions of epoxyeicosatrienoic acids (EETs). (wikipathways.org)
Protein4
- We offer Fumarylacetoacetate hydrolase Peptides and Fumarylacetoacetate hydrolase Proteins for use in common research applications: ELISA, Protein Array, Western Blot. (novusbio.com)
- Each Fumarylacetoacetate hydrolase Peptide and Fumarylacetoacetate hydrolase Protein is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. (novusbio.com)
- Staphylococcus aureus produces two major PG hydrolases: major autolysin (Atl) and Aaa, a autolysin/ adhesin protein. (amrita.edu)
- The Peptidyl-tRNA Hydrolase 2 (PTRH2) gene codes for a highly conserved mitochondrial protein. (cags.org.ae)
Inhibitors2
- Recent development of inhibitors of the soluble epoxide hydrolase are proving to have potent anti-inflammatory, anti-hypertensive, and anti-nociceptive properties. (wikipathways.org)
- Augmentation of levels of endocannabinoids with inhibitors of fatty acid amide hydrolase (FAAH) is analgesic in models of acute and inflammatory pain states. (jneurosci.org)
FAAH4
- Fatty acid amide hydrolase (FAAH) terminates the endocannabinoid signaling pathway that regulates numerous neurobehavioral processes in animals by hydrolyzing a class of lipid mediators, N-acylethanolamines (NAEs). (unt.edu)
- The activity of AEA at its receptors is limited by cellular uptake through a specific membrane transporter, followed by intracellular degradation by a fatty acid amide hydrolase (FAAH). (ingentaconnect.com)
- The signaling function of NAE is terminated by fatty acid amide hydrolase (FAAH), which hydrolyzes NAE to ethanolamine and free fatty acid. (frontiersin.org)
- Here we provide evidence that a critical balance between anandamide synthesis by N-acylphosphatidylethanolamine-selective phospholipase D (NAPE-PLD) and its degradation by fatty acid amide hydrolase (FAAH) in mouse embryos and oviducts creates locally an appropriate "anandamide tone" for normal development of embryos and their oviductal transport. (ovid.com)
Gene2
- To better understand the basis of this system, spontaneous glycosyl hydrolase mutants were isolated using a genetic screen for mutations, which reduced expression of the lacS gene. (genetics.org)
- We conclude that the early flowering phenotype of AtFAAH overexpressors is, in part, explained by elevated FT gene expression resulting from the enhanced NAE hydrolase activity of AtFAAH, suggesting that NAE metabolism may participate in floral signaling pathways. (frontiersin.org)
Fatty4
- Fatty Acid Amide Hydrolase: A Potential Target for Next Generatio. (ingentaconnect.com)
- Fatty-acid amide hydrolase 1 (FAAH1) is a plasma membrane-bound hydrolase that converts oleamide to oleic acid (2). (cellsignal.com)
- This hydrolase also converts the cannabinoid anandamide, the endogenous ligand for the CB1 cannabinoid receptor, to arachidonic acid, suggesting a role in fatty-acid amide inactivation (2). (cellsignal.com)
- With the exception of the 5(6)-EET, these epoxy fatty acids are good substrates for the soluble epoxide hydrolase. (wikipathways.org)
Fumarylacetoacetate hydrolase2
- Our Fumarylacetoacetate hydrolase Peptides and Fumarylacetoacetate hydrolase Proteins can be used in a variety of model species: Human. (novusbio.com)
- Choose from our Fumarylacetoacetate hydrolase Peptides and Proteins. (novusbio.com)
Peptide5
- Think about an active hydrolase which breaks down peptide bonds. (biology-online.org)
- A hydrolase which breaks down peptide bonds would break down its bonds as well. (biology-online.org)
- 1. Uncentrifuged and centrifuged rat intestinal contents were assayed for peptide hydrolase activity with glycyl-l-phenylalanine (Gly-Phe) and l-phenylalanyl-glycine (Phe-Gly) as substrates in the absence and presence of the intestinal cytosol peptide hydrolase inhibitor p -hydroxymercuribenzoate. (portlandpress.com)
- 3. p -Hydroxymercuribenzoate markedly inhibited jejunal peptide hydrolase activity. (portlandpress.com)
- Although the presence of soluble bacterial enzymes cannot be excluded, peptide hydrolase enzymes in jejunal contents have the characteristics of mucosal cytosol enzymes whereas enzymes in ileal contents have the characteristics of mucosal brush border as well as cytosol enzymes. (portlandpress.com)
Hydrolytic4
- These unique hydrolases are scarcely studied for biocatalytical applications in organic chemistry yet, although many other hydrolytic enzymes (e.g. lipases) are commonly applied as catalysts. (springer.com)
- The alpha/beta hydrolase fold common to several hydrolytic enzymes of differing phylogenetic origin and catalytic function was described by Ollis et al. (google.com.au)
- In order to address this problem, this paper reports the application of a 96-well microplate fluorogenic assay, originally designed for purified hydrolytic enzymes, 12,13 to screen microbial whole cells for epoxide hydrolases (EHs) and Baeyer-Villiger monooxygenases (BVMOs). (scielo.br)
- The karrikin-1 (KAR1) ligand sits in the opening to the active site abutting a helical domain insert but distal from the canonical catalytic triad (Ser95-His246-Asp217) of α/β-hydrolases, consistent with the lack of detectable hydrolytic activity by purified KAI2. (sigmaaldrich.com)
Hydrolysis7
- Glycoside hydrolases (also called glycosidases or glycosyl hydrolases ) catalyze the hydrolysis of glycosidic bonds in complex sugars . (wikipedia.org)
- Glycoside hydrolases are classified into EC 3.2.1 as enzymes catalyzing the hydrolysis of O- or S-glycosides. (wikipedia.org)
- Glycoside hydrolases can also be classified according to the stereochemical outcome of the hydrolysis reaction: thus they can be classified as either retaining or inverting enzymes. (wikipedia.org)
- Phosphoric monoester hydrolases (or phosphomonoesterases) are enzymes that catalyse the hydrolysis of O-P bonds by nucleophilic attack of phosphorus by cysteine residues or coordinated metal ions. (wikipedia.org)
- Acid anhydride hydrolases are a class of hydrolase enzymes that catalyze the hydrolysis of an acid anhydride bond. (wikipedia.org)
- Interestingly, bacteria have also been reported to detoxify ITC hydrolysis products, but in a manner that is remarkably distinct from those in herbivores, by directly degrading ITCs into carbonyl sulfide and their corresponding amines using metallo-β-lactamase (MBL) enzymes named ITC hydrolases (ITCases) 18 , 19 . (nature.com)
- In this thesis, fungal glycoside hydrolases, cellulases and hemicellulases were studied with the aim of increasing our knowledge of the mechanisms involved in the enzymatic hydrolysis of cellulose and lignocellulose. (lu.se)
Organism1
- 1) A hydrolase which binds other hydrolase molecules of its type may have gotten eliminated thru natural selection if it did not impart any evolutionary advantage to the organism. (biology-online.org)
Ester hydrolase activity1
- Seventeen strains of lactic acid bacteria were assayed for their glycerol ester hydrolase activity by using an improved agar-well technique, and eight strains by determining the activity in cell-free extracts using a p H-stat procedure. (asm.org)
Catalytic3
- In this field, hydrolases have been mainly studied due to their commercial availability, high stability, broad substrate scope and high catalytic efficiency in media containing organic solvents. (rsc.org)
- To understand how the modular architecture of SH3b and NlpC/P60 affects the activity of the catalytic domain, three putative NlpC/P60 cell wall hydrolases were biochemically and structurally characterized. (osti.gov)
- Maslova O, Aslanli A, Stepanov N, Lyagin I, Efremenko E. Catalytic Characteristics of New Antibacterials Based on Hexahistidine-Containing Organophosphorus Hydrolase. (mdpi.com)
Superfamily1
- Several hydrolases of the SGNH superfamily, including the lipase SrLip from Streptomyces rimosus (Q93MW7), the acyl-CoA thioesterase I TesA from Pseudomonas aeruginosa (Q9HZY8) and the two lipolytic enzymes EstA (from P. aeruginosa, O33407) and EstP (from Pseudomonas putida, Q88QS0), were examined for promiscuity. (semanticscholar.org)
Catalyze3
- Acylpeptide hydrolases (APHs) catalyze the removal of N -acylated amino acids from blocked peptides. (mdpi.com)
- Peptidoglycan (PG) hydrolases or autolysins are a group of enzymes which catalyze the degradation of bacterial cell wall at specific sites. (amrita.edu)
- The transglycosylases, hydrolases which naturally catalyze mainly transfer reactions, are of special interest and might be useful guides for engineering of other hydrolases. (lu.se)
Antibodies2
- Listed below are anti-LTA4 Hydrolase antibodies from multiple suppliers. (biocompare.com)
- Your search returned 46 LTA4 Hydrolase Antibodies across 9 suppliers. (biocompare.com)
Activity11
- Polysaccharide hydrolase activity was assayed in a group of 28 selected Rhizopus strains. (springer.com)
- Estimation of the uptake potential of rabbit alveolar macrophages is performed in-vitro using P-32 labeled bacteria, with the lytic potential of the same cells prior to phagocytosis estimated from the activity of six lysosomal hydrolase enzymes and related to macrophages as a means for comparison with uptake activities. (cdc.gov)
- Results: Compared to the low-activity microsomal epoxide hydrolase genotype stratum, the odds ratio for the presence of the VC-induced mutant biomarkers increased to 1.16 (95% CI: 0.64-2.10) in the medium-activity genotype stratum and to 1.35 (95% CI: 0.66-2.77) in the high-activity genotype stratum. (cdc.gov)
- Recent studies show increased activity of soluble epoxide hydrolase (sEH) during obesity and metabolic dysfunction. (hindawi.com)
- The activity of some pneumococcal murein hydrolases (MHs) appears to be constrained by the membrane lipoteichoic acid (LTA) at the posttranslational level. (asmscience.org)
- The importance of BLM hydrolase in limiting the therapeutic effectiveness of BLM was examined by measuring BLM hydrolase activity and the response of human tumors to BLM in culture. (aacrjournals.org)
- No correlation was observed, however, between BLM hydrolase activity and response to BLM in soft agar. (aacrjournals.org)
- LTA4 hydrolase activity was present in the 15,000 x g and the 100,000 x g supernatants and was inactivated by heating at 56 degrees C or by pronase, as is the case for neutrophil LTA4 hydrolase. (ovid.com)
- Anion exchange chromatography of epithelial cell samples revealed that LTA4 hydrolase and aminopeptidase activity did not co-elute, whereas one of three peaks of aminopeptidase activity did co-elute in chromatograms of neutrophil samples. (ovid.com)
- Repeat kinetic analysis on 179-fold purified epithelial LTA4 hydrolase again revealed that it lacked significant aminopeptidase activity and retained its unique kinetic properties. (ovid.com)
- Ubiquitin C-terminal hydrolase-L3 promotes interferon antiviral activity by stabilizing type I-interferon receptor. (sigmaaldrich.com)
Degradation2
- In higher organisms glycoside hydrolases are found within the endoplasmic reticulum and Golgi apparatus where they are involved in processing of N-linked glycoproteins , and in the lysosome as enzymes involved in the degradation of carbohydrate structures. (wikipedia.org)
- The glycoside hydrolases are involved in the biosynthesis and degradation of glycogen in the body. (wikipedia.org)
Epoxide hydrolases2
- Biocatalysis reactions were performed on microtiter plates (200 µL) aiming at the utilization of fluorogenic substrates (100 µmol L -1 ) for rapid whole cell screening for epoxide hydrolases (EHs) and Baeyer-Villiger monoxygenases (BVMOs). (scielo.br)
- Gerry Brooks and epoxide hydrolases: four decades to a pharmaceutical. (wikipathways.org)
Glycosyl hydrolases1
- A classification system for glycosyl hydrolases, based on sequence similarity, has led to the definition of more than 100 different families. (wikipedia.org)
Bacterial hydrolases1
- The CW is remodelled by bacterial hydrolases, whose activities are carefully regulated to maintain cell integrity or lead to bacterial death. (frontiersin.org)
Peptidoglycan hydrolases3
- Most bacteria have multiple peptidoglycan hydrolases capable of cleaving covalent bonds in peptidoglycan sacculi or its fragments. (nih.gov)
- An overview of the different classes of peptidoglycan hydrolases and their cleavage sites is provided. (nih.gov)
- Moreover, peptidoglycan hydrolases are involved in lysis phenomena such as fratricide or developmental lysis occurring in bacterial populations. (nih.gov)
Bacteria1
- These results also suggest that new pathways for recycling PG turnover products, such as tracheal cytotoxin, may have evolved in bacteria in the human gut microbiome that involve NlpC/P60 cell wall hydrolases. (osti.gov)
Microsomal4
- Polymorphisms of microsomal epoxide hydrolase in French vinyl chloride workers. (cdc.gov)
- Conclusions: The results suggest that polymorphisms in microsomal epoxide hydrolase do not play a significant role in susceptibility to the mutagenic effects of vinyl chloride. (cdc.gov)
- Microsomal epoxide hydrolase (epoxide hydrolase 1, EH1, or mEH), soluble epoxide hydrolase (sEH, epoxide hydrolase 2, EH2, or cytoplasmic epoxide hydrolase), and the more recently discovered but not as yet well defined functionally, epoxide hydrolase 3 (EH3) and epoxide hydrolase 4 (EH4) are structurally closely related isozymes . (wikidoc.org)
- mEH therefore may play a minor role, compared to sEH, in limiting the bioactivity of these cell signaling compounds (see microsomal epoxide hydrolase ). (wikidoc.org)
EPHX21
- EPHX2 (soluble Epoxide Hydrolase 2, sEH) converts biologically active epoxyeicosatrienoic acids (EETs), anti-inflammatory and pro-fibrinolytic effectors into the less biologically active metabolites, dihydroxyeicostrienoic acids. (diabetesjournals.org)
Substrates1
- C. perfringens produces a battery of secreted carbohydrate-active enzymes called glycoside hydrolases whose activities suggest that their substrates are complex eukaryotic glycans, such as those found in the mucosal layer of the gut. (queensu.ca)
Lysosomal2
- Deficiency in specific lysosomal glycoside hydrolases can lead to a range of lysosomal storage disorders that result in developmental problems or death. (wikipedia.org)
- Lysosomal hydrolases of the epidermis. (nih.gov)
Metabolism1
- Given that DHA metabolism by cytochrome P450 and soluble epoxide hydrolase (sEH) enzymes affects retinal angiogenesis and vascular stability, we investigated the role of sEH in a mouse model of ROP. (jci.org)
Classification2
- Recent progress in glycosidase sequence analysis and 3D structure comparison has allowed the proposal of an extended hierarchical classification of the glycoside hydrolases. (wikipedia.org)
- Hydrolases are classified as EC 3 in the EC number classification of enzymes. (wikidoc.org)
Arabidopsis2
- Smoke-derived karrikin perception by the α/β-hydrolase KAI2 from Arabidopsis. (sigmaaldrich.com)
- Genetic studies in Arabidopsis implicate an α/β-hydrolase, KARRIKIN-INSENSITIVE 2 (KAI2) as a receptor for karrikins, germination-promoting butenolide small molecules found in the smoke of burned plants. (sigmaaldrich.com)
Epoxyeicosatrienoic acids2
- By diminishing the anti-inflammatory activities of epoxyeicosatrienoic acids (EETs), soluble epoxide hydrolase (sEH) has been considered a potential therapeutic target for epileptic seizures. (omicsonline.org)
- The soluble epoxide hydrolase (sEH), which is expressed in pulmonary artery smooth muscle cells, metabolizes cytochrome P450 (CYP) epoxygenase-derived epoxyeicosatrienoic acids (EETs) to their less active diols. (ahajournals.org)
Intestinal3
- Glycoside hydrolases are found in the intestinal tract and in saliva where they degrade complex carbohydrates such as lactose , starch , sucrose and trehalose . (wikipedia.org)
- Properties of food folates determined by stability and susceptibility to intestinal pteroylpolyglutamate hydrolase action. (biomedsearch.com)
- On the other hand, the presence of polyglutamyl folate necessitates the action of intestinal hydrolases, which could be affected by food constituents. (biomedsearch.com)
Acid anhydride4
- Acid Anhydride Hydrolases: Advances in Research and Application. (wikipedia.org)
- Acid Anhydride Hydrolases" is a descriptor in the National Library of Medicine's controlled vocabulary thesaurus, MeSH (Medical Subject Headings) . (umassmed.edu)
- This graph shows the total number of publications written about "Acid Anhydride Hydrolases" by people in this website by year, and whether "Acid Anhydride Hydrolases" was a major or minor topic of these publications. (umassmed.edu)
- Below are the most recent publications written about "Acid Anhydride Hydrolases" by people in Profiles. (umassmed.edu)
Amino3
- The present invention provides amino acid sequences for the hydrolase. (google.com.au)
- The present invention provides the nucleic acid and amino acid sequences for the hydrolases and methods for their use. (google.com.au)
- Recombinant fragment, corresponding to a region within amino acids 1-193 of Human Epoxide hydrolase (NP_000111). (abcam.com)
Reactivity1
- Retro-Friedel-Crafts hydrolases are co-factor independent enzymes with unusual reactivity and selectivity. (springer.com)
Organophosphorus1
- To date, the binding pose of organophosphorus (OP) compounds of APH, as well as the different OP compounds binding and inducing conformational changes in two domains, namely, α/β hydrolase and β-propeller, remain poorly understood. (mdpi.com)
Nucleic2
- The present invention provides host cells which comprise nucleic acid encoding the hydrolase. (google.com.au)
- For example, a nuclease is a hydrolase that cleaves nucleic acids. (wikidoc.org)
Polysaccharide1
- [3] Glycoside hydrolases can also be classified as exo or endo acting, dependent upon whether they act at the (usually non-reducing) end or in the middle, respectively, of an oligo/polysaccharide chain. (wikipedia.org)