AnatomyOrganismsDiseasesChemicals and DrugsAnalytical, Diagnostic and Therapeutic Techniques and EquipmentPhenomena and ProcessesInformation ScienceHealth Care
HybridomasAntibodies, MonoclonalAntibody SpecificityEpitopesMice, Inbred BALB CT-LymphocytesImmunoglobulin Variable RegionImmunoglobulin Heavy ChainsB-LymphocytesHistocompatibility Antigens Class IIPlasmacytomaCell FusionHybrid CellsBinding Sites, AntibodyCross ReactionsImmunoglobulin Light ChainsImmunoglobulin IdiotypesGenes, ImmunoglobulinImmunoglobulin kappa-ChainsLymphocyte ActivationReceptors, Antigen, T-CellClone CellsAntigens, SurfaceSuppressor Factors, ImmunologicMice, Inbred StrainsEnzyme-Linked Immunosorbent AssayMolecular Sequence DataCell LineSpleenInterleukin-2Immunoglobulin MImmunoglobulin AllotypesAmino Acid SequenceLymphocyte CooperationMice, Inbred CBAAntigen-Antibody ReactionsHistoplasminImmunoglobulin GAntigensImmunoglobulin mu-ChainsAntigen-Antibody ComplexAntigen-Presenting CellsMajor Histocompatibility Complexp-AzobenzenearsonateAntibody AffinityH-2 AntigensGenes, MHC Class IIAntibody DiversityAutoantibodiesReceptors, Antigen, T-Cell, alpha-betaBase SequenceMice, Inbred C57BLPhenylacetatesFluorescent Antibody TechniqueAntigen PresentationThymomaMice, Inbred AProstatic Secretory ProteinsImmunoglobulin lambda-ChainsTrinitrobenzenesMice, Inbred NZBGene Rearrangement, B-Lymphocyte, Light ChainMice, Inbred AKRAntibody FormationImmunizationLymphokinesImmunoglobulin IsotypesAntibodies, AntinuclearAntibodies, Anti-IdiotypicAntigens, CD5Myeloma ProteinsAntibodies, HeterophileAutoantigensImmunoglobulinsMuramidaseAntigens, NeoplasmAntigens, ViralGene Rearrangement, B-Lymphocyte
Hybridomas
Cells artificially created by fusion of activated lymphocytes with neoplastic cells. The resulting hybrid cells are cloned and produce pure MONOCLONAL ANTIBODIES or T-cell products, identical to those produced by the immunologically competent parent cell.
Antibodies, Monoclonal
Antibodies produced by a single clone of cells.
Antibody Specificity
The property of antibodies which enables them to react with some ANTIGENIC DETERMINANTS and not with others. Specificity is dependent on chemical composition, physical forces, and molecular structure at the binding site.
Epitopes
Sites on an antigen that interact with specific antibodies.
Mice, Inbred BALB C
T-Lymphocytes
Lymphocytes responsible for cell-mediated immunity. Two types have been identified - cytotoxic (T-LYMPHOCYTES, CYTOTOXIC) and helper T-lymphocytes (T-LYMPHOCYTES, HELPER-INDUCER). They are formed when lymphocytes circulate through the THYMUS GLAND and differentiate to thymocytes. When exposed to an antigen, they divide rapidly and produce large numbers of new T cells sensitized to that antigen.
Immunoglobulin Variable Region
That region of the immunoglobulin molecule that varies in its amino acid sequence and composition, and comprises the binding site for a specific antigen. It is located at the N-terminus of the Fab fragment of the immunoglobulin. It includes hypervariable regions (COMPLEMENTARITY DETERMINING REGIONS) and framework regions.
Immunoglobulin Heavy Chains
The largest of polypeptide chains comprising immunoglobulins. They contain 450 to 600 amino acid residues per chain, and have molecular weights of 51-72 kDa.
B-Lymphocytes
Lymphoid cells concerned with humoral immunity. They are short-lived cells resembling bursa-derived lymphocytes of birds in their production of immunoglobulin upon appropriate stimulation.
Histocompatibility Antigens Class II
Large, transmembrane, non-covalently linked glycoproteins (alpha and beta). Both chains can be polymorphic although there is more structural variation in the beta chains. The class II antigens in humans are called HLA-D ANTIGENS and are coded by a gene on chromosome 6. In mice, two genes named IA and IE on chromosome 17 code for the H-2 antigens. The antigens are found on B-lymphocytes, macrophages, epidermal cells, and sperm and are thought to mediate the competence of and cellular cooperation in the immune response. The term IA antigens used to refer only to the proteins encoded by the IA genes in the mouse, but is now used as a generic term for any class II histocompatibility antigen.
Plasmacytoma
Any discrete, presumably solitary, mass of neoplastic PLASMA CELLS either in BONE MARROW or various extramedullary sites.
Cell Fusion
Fusion of somatic cells in vitro or in vivo, which results in somatic cell hybridization.
Hybrid Cells
Any cell, other than a ZYGOTE, that contains elements (such as NUCLEI and CYTOPLASM) from two or more different cells, usually produced by artificial CELL FUSION.
Binding Sites, Antibody
Local surface sites on antibodies which react with antigen determinant sites on antigens (EPITOPES.) They are formed from parts of the variable regions of FAB FRAGMENTS.
Cross Reactions
Serological reactions in which an antiserum against one antigen reacts with a non-identical but closely related antigen.
Immunoglobulin Light Chains
Polypeptide chains, consisting of 211 to 217 amino acid residues and having a molecular weight of approximately 22 kDa. There are two major types of light chains, kappa and lambda. Two Ig light chains and two Ig heavy chains (IMMUNOGLOBULIN HEAVY CHAINS) make one immunoglobulin molecule.
Immunoglobulin Idiotypes
Unique genetically-controlled determinants present on ANTIBODIES whose specificity is limited to a single group of proteins (e.g., another antibody molecule or an individual myeloma protein). The idiotype appears to represent the antigenicity of the antigen-binding site of the antibody and to be genetically codetermined with it. The idiotypic determinants have been precisely located to the IMMUNOGLOBULIN VARIABLE REGION of both immunoglobin polypeptide chains.
Genes, Immunoglobulin
Genes encoding the different subunits of the IMMUNOGLOBULINS, for example the IMMUNOGLOBULIN LIGHT CHAIN GENES and the IMMUNOGLOBULIN HEAVY CHAIN GENES. The heavy and light immunoglobulin genes are present as gene segments in the germline cells. The completed genes are created when the segments are shuffled and assembled (B-LYMPHOCYTE GENE REARRANGEMENT) during B-LYMPHOCYTE maturation. The gene segments of the human light and heavy chain germline genes are symbolized V (variable), J (joining) and C (constant). The heavy chain germline genes have an additional segment D (diversity).
Immunoglobulin kappa-Chains
One of the types of light chains of the immunoglobulins with a molecular weight of approximately 22 kDa.
Lymphocyte Activation
Morphologic alteration of small B LYMPHOCYTES or T LYMPHOCYTES in culture into large blast-like cells able to synthesize DNA and RNA and to divide mitotically. It is induced by INTERLEUKINS; MITOGENS such as PHYTOHEMAGGLUTININS, and by specific ANTIGENS. It may also occur in vivo as in GRAFT REJECTION.
Receptors, Antigen, T-Cell
Molecules on the surface of T-lymphocytes that recognize and combine with antigens. The receptors are non-covalently associated with a complex of several polypeptides collectively called CD3 antigens (ANTIGENS, CD3). Recognition of foreign antigen and the major histocompatibility complex is accomplished by a single heterodimeric antigen-receptor structure, composed of either alpha-beta (RECEPTORS, ANTIGEN, T-CELL, ALPHA-BETA) or gamma-delta (RECEPTORS, ANTIGEN, T-CELL, GAMMA-DELTA) chains.
Clone Cells
A group of genetically identical cells all descended from a single common ancestral cell by mitosis in eukaryotes or by binary fission in prokaryotes. Clone cells also include populations of recombinant DNA molecules all carrying the same inserted sequence. (From King & Stansfield, Dictionary of Genetics, 4th ed)
Antigens, Surface
Antigens on surfaces of cells, including infectious or foreign cells or viruses. They are usually protein-containing groups on cell membranes or walls and may be isolated.
Suppressor Factors, Immunologic
Proteins, protein complexes, or glycoproteins secreted by suppressor T-cells that inhibit either subsequent T-cells, B-cells, or other immunologic phenomena. Some of these factors have both histocompatibility (I-J) and antigen-specific domains which may be linked by disulfide bridges. They can be elicited by haptens or other antigens and may be mass-produced by hybridomas or monoclones in the laboratory.
Mice, Inbred Strains
Genetically identical individuals developed from brother and sister matings which have been carried out for twenty or more generations, or by parent x offspring matings carried out with certain restrictions. All animals within an inbred strain trace back to a common ancestor in the twentieth generation.
Enzyme-Linked Immunosorbent Assay
An immunoassay utilizing an antibody labeled with an enzyme marker such as horseradish peroxidase. While either the enzyme or the antibody is bound to an immunosorbent substrate, they both retain their biologic activity; the change in enzyme activity as a result of the enzyme-antibody-antigen reaction is proportional to the concentration of the antigen and can be measured spectrophotometrically or with the naked eye. Many variations of the method have been developed.
Molecular Sequence Data
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
Cell Line
Established cell cultures that have the potential to propagate indefinitely.
Spleen
An encapsulated lymphatic organ through which venous blood filters.
Interleukin-2
A soluble substance elaborated by antigen- or mitogen-stimulated T-LYMPHOCYTES which induces DNA synthesis in naive lymphocytes.
Immunoglobulin M
A class of immunoglobulin bearing mu chains (IMMUNOGLOBULIN MU-CHAINS). IgM can fix COMPLEMENT. The name comes from its high molecular weight and originally being called a macroglobulin.
Immunoglobulin Allotypes
Allelic variants of the immunoglobulin light chains (IMMUNOGLOBULIN LIGHT CHAINS) or heavy chains (IMMUNOGLOBULIN HEAVY CHAINS) encoded by ALLELES of IMMUNOGLOBULIN GENES.
Amino Acid Sequence
The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.
Lymphocyte Cooperation
T-cell enhancement of the B-cell response to thymic-dependent antigens.
Mice, Inbred CBA
Antigen-Antibody Reactions
The processes triggered by interactions of ANTIBODIES with their ANTIGENS.
Histoplasmin
Immunoglobulin G
The major immunoglobulin isotype class in normal human serum. There are several isotype subclasses of IgG, for example, IgG1, IgG2A, and IgG2B.
Antigens
Substances that are recognized by the immune system and induce an immune reaction.
Immunoglobulin mu-Chains
The class of heavy chains found in IMMUNOGLOBULIN M. They have a molecular weight of approximately 72 kDa and they contain about 57 amino acid residues arranged in five domains and have more oligosaccharide branches and a higher carbohydrate content than the heavy chains of IMMUNOGLOBULIN G.
Antigen-Antibody Complex
The complex formed by the binding of antigen and antibody molecules. The deposition of large antigen-antibody complexes leading to tissue damage causes IMMUNE COMPLEX DISEASES.
Antigen-Presenting Cells
A heterogeneous group of immunocompetent cells that mediate the cellular immune response by processing and presenting antigens to the T-cells. Traditional antigen-presenting cells include MACROPHAGES; DENDRITIC CELLS; LANGERHANS CELLS; and B-LYMPHOCYTES. FOLLICULAR DENDRITIC CELLS are not traditional antigen-presenting cells, but because they hold antigen on their cell surface in the form of IMMUNE COMPLEXES for B-cell recognition they are considered so by some authors.
Major Histocompatibility Complex
The genetic region which contains the loci of genes which determine the structure of the serologically defined (SD) and lymphocyte-defined (LD) TRANSPLANTATION ANTIGENS, genes which control the structure of the IMMUNE RESPONSE-ASSOCIATED ANTIGENS, HUMAN; the IMMUNE RESPONSE GENES which control the ability of an animal to respond immunologically to antigenic stimuli, and genes which determine the structure and/or level of the first four components of complement.
p-Azobenzenearsonate
A hapten capable of eliciting both antibody formation and delayed hypersensitivity when bound to aromatic amino acids, polypeptides or proteins. It is used as an immunologic research tool.
Antibody Affinity
A measure of the binding strength between antibody and a simple hapten or antigen determinant. It depends on the closeness of stereochemical fit between antibody combining sites and antigen determinants, on the size of the area of contact between them, and on the distribution of charged and hydrophobic groups. It includes the concept of "avidity," which refers to the strength of the antigen-antibody bond after formation of reversible complexes.
H-2 Antigens
The major group of transplantation antigens in the mouse.
Genes, MHC Class II
Genetic loci in the vertebrate major histocompatibility complex that encode polymorphic products which control the immune response to specific antigens. The genes are found in the HLA-D region in humans and in the I region in mice.
Antibody Diversity
The phenomenon of immense variability characteristic of ANTIBODIES. It enables the IMMUNE SYSTEM to react specifically against the essentially unlimited kinds of ANTIGENS it encounters. Antibody diversity is accounted for by three main theories: (1) the Germ Line Theory, which holds that each antibody-producing cell has genes coding for all possible antibody specificities, but expresses only the one stimulated by antigen; (2) the Somatic Mutation Theory, which holds that antibody-producing cells contain only a few genes, which produce antibody diversity by mutation; and (3) the Gene Rearrangement Theory, which holds that antibody diversity is generated by the rearrangement of IMMUNOGLOBULIN VARIABLE REGION gene segments during the differentiation of the ANTIBODY-PRODUCING CELLS.
Autoantibodies
Antibodies that react with self-antigens (AUTOANTIGENS) of the organism that produced them.
Receptors, Antigen, T-Cell, alpha-beta
T-cell receptors composed of CD3-associated alpha and beta polypeptide chains and expressed primarily in CD4+ or CD8+ T-cells. Unlike immunoglobulins, the alpha-beta T-cell receptors recognize antigens only when presented in association with major histocompatibility (MHC) molecules.
Base Sequence
The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.
Mice, Inbred C57BL
Phenylacetates
Derivatives of phenylacetic acid. Included under this heading are a variety of acid forms, salts, esters, and amides that contain the benzeneacetic acid structure. Note that this class of compounds should not be confused with derivatives of phenyl acetate, which contain the PHENOL ester of ACETIC ACID.
Fluorescent Antibody Technique
Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
Antigen Presentation
The process by which antigen is presented to lymphocytes in a form they can recognize. This is performed by antigen presenting cells (APCs). Some antigens require processing before they can be recognized. Antigen processing consists of ingestion and partial digestion of the antigen by the APC, followed by presentation of fragments on the cell surface. (From Rosen et al., Dictionary of Immunology, 1989)
Thymoma
A neoplasm originating from thymic tissue, usually benign, and frequently encapsulated. Although it is occasionally invasive, metastases are extremely rare. It consists of any type of thymic epithelial cell as well as lymphocytes that are usually abundant. Malignant lymphomas that involve the thymus, e.g., lymphosarcoma, Hodgkin's disease (previously termed granulomatous thymoma), should not be regarded as thymoma. (From Stedman, 25th ed)
Mice, Inbred A
Prostatic Secretory Proteins
Proteins secreted by the prostate gland. The major secretory proteins from the human prostate gland include PROSTATE-SPECIFIC ANTIGEN, prostate-specific acid phosphatase, prostate-specific membrane antigen, and prostate-specific protein-94.
Immunoglobulin lambda-Chains
One of the types of light chain subunits of the immunoglobulins with a molecular weight of approximately 22 kDa.
Trinitrobenzenes
Benzene derivatives which are substituted with three nitro groups in any position.
Mice, Inbred NZB
Gene Rearrangement, B-Lymphocyte, Light Chain
Ordered rearrangement of B-lymphocyte variable gene regions coding for the kappa or lambda IMMUNOGLOBULIN LIGHT CHAINS, thereby contributing to antibody diversity. It occurs during the second stage of differentiation of the IMMATURE B-LYMPHOCYTES.
Mice, Inbred AKR
Antibody Formation
The production of ANTIBODIES by proliferating and differentiated B-LYMPHOCYTES under stimulation by ANTIGENS.
Immunization
Deliberate stimulation of the host's immune response. ACTIVE IMMUNIZATION involves administration of ANTIGENS or IMMUNOLOGIC ADJUVANTS. PASSIVE IMMUNIZATION involves administration of IMMUNE SERA or LYMPHOCYTES or their extracts (e.g., transfer factor, immune RNA) or transplantation of immunocompetent cell producing tissue (thymus or bone marrow).
Lymphokines
Soluble protein factors generated by activated lymphocytes that affect other cells, primarily those involved in cellular immunity.
Immunoglobulin Isotypes
The classes of immunoglobulins found in any species of animal. In man there are nine classes that migrate in five different groups in electrophoresis; they each consist of two light and two heavy protein chains, and each group has distinguishing structural and functional properties.
Antibodies, Antinuclear
Autoantibodies directed against various nuclear antigens including DNA, RNA, histones, acidic nuclear proteins, or complexes of these molecular elements. Antinuclear antibodies are found in systemic autoimmune diseases including systemic lupus erythematosus, Sjogren's syndrome, scleroderma, polymyositis, and mixed connective tissue disease.
Antibodies, Anti-Idiotypic
Antibodies which react with the individual structural determinants (idiotopes) on the variable region of other antibodies.
Antigens, CD5
Glycoproteins expressed on all mature T-cells, thymocytes, and a subset of mature B-cells. Antibodies specific for CD5 can enhance T-cell receptor-mediated T-cell activation. The B-cell-specific molecule CD72 is a natural ligand for CD5. (From Abbas et al., Cellular and Molecular Immunology, 2d ed, p156)
Myeloma Proteins
Abnormal immunoglobulins characteristic of MULTIPLE MYELOMA.
Antibodies, Heterophile
Antibodies elicited in a different species from which the antigen originated. These antibodies are directed against a wide variety of interspecies-specific antigens, the best known of which are Forssman, Hanganutziu-Deicher (H-D), and Paul-Bunnell (P-B). Incidence of antibodies to these antigens--i.e., the phenomenon of heterophile antibody response--is useful in the serodiagnosis, pathogenesis, and prognosis of infection and latent infectious states as well as in cancer classification.
Autoantigens
Endogenous tissue constituents that have the ability to interact with AUTOANTIBODIES and cause an immune response.
Immunoglobulins
Multi-subunit proteins which function in IMMUNITY. They are produced by B LYMPHOCYTES from the IMMUNOGLOBULIN GENES. They are comprised of two heavy (IMMUNOGLOBULIN HEAVY CHAINS) and two light chains (IMMUNOGLOBULIN LIGHT CHAINS) with additional ancillary polypeptide chains depending on their isoforms. The variety of isoforms include monomeric or polymeric forms, and transmembrane forms (B-CELL ANTIGEN RECEPTORS) or secreted forms (ANTIBODIES). They are divided by the amino acid sequence of their heavy chains into five classes (IMMUNOGLOBULIN A; IMMUNOGLOBULIN D; IMMUNOGLOBULIN E; IMMUNOGLOBULIN G; IMMUNOGLOBULIN M) and various subclasses.
Muramidase
A basic enzyme that is present in saliva, tears, egg white, and many animal fluids. It functions as an antibacterial agent. The enzyme catalyzes the hydrolysis of 1,4-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrin. EC 3.2.1.17.
Antigens, Neoplasm
Proteins, glycoprotein, or lipoprotein moieties on surfaces of tumor cells that are usually identified by monoclonal antibodies. Many of these are of either embryonic or viral origin.
Antigens, Viral
Substances elaborated by viruses that have antigenic activity.
Gene Rearrangement, B-Lymphocyte
Ordered rearrangement of B-lymphocyte variable gene regions coding for the IMMUNOGLOBULIN CHAINS, thereby contributing to antibody diversity. It occurs during the differentiation of the IMMATURE B-LYMPHOCYTES.

Repertoire of human antibodies against the polysaccharide capsule of Streptococcus pneumoniae serotype 6B. (1/2670)

We examined the repertoire of antibodies to Streptococcus pneumoniae 6B capsular polysaccharide induced with the conventional polysaccharide vaccine in adults at the molecular level two ways. In the first, we purified from the sera of seven vaccinees antipneumococcal antibodies and determined their amino acid sequences. Their VH regions are mainly the products of VH3 family genes (candidate genes, 3-23, 3-07, 3-66, and 3-74), but the product of a VH1 family gene (candidate gene, 1-03) is occasionally used. All seven individuals have small amounts of polyclonal kappa+ antibodies (Vkappa1 to Vkappa4 families), although kappa+ antibodies are occasionally dominated by antibodies formed with the product of the A27 Vkappa gene. In contrast, lambda+ anti-6B antibodies are dominated by the antibodies derived from one of 3 very similar Vlambda2 family genes (candidate genes, 2c, 2e, and 2a2) and Clambda1 gene product. The Vlambda2(+) antibodies express the 8.12 idiotype, which is expressed on anti-double-stranded-DNA antibodies. In one case, Vlambda is derived from a rarely expressed Vlambda gene, 10a. In the second approach, we studied a human hybridoma (Dob1) producing anti-6B antibody. Its VH region sequence is closely related to those of the 3-15 VH gene (88% nucleotide homology) and JH4 (92% homology). Its VL region is homologous to the 2a2 Vlambda2 gene (91%) and Jlambda1/Clambda1. Taken together, the V region of human anti-6B antibodies is commonly formed by a VH3 and a Vlambda2 family gene product.  (+info)

Mechanisms of double-strand-break repair during gene targeting in mammalian cells. (2/2670)

In the present study, the mechanism of double-strand-break (DSB) repair during gene targeting at the chromosomal immunoglobulin mu-locus in a murine hybridoma was examined. The gene-targeting assay utilized specially designed insertion vectors genetically marked in the region of homology to the chromosomal mu-locus by six diagnostic restriction enzyme site markers. The restriction enzyme markers permitted the contribution of vector-borne and chromosomal mu-sequences in the recombinant product to be determined. The use of the insertion vectors in conjunction with a plating procedure in which individual integrative homologous recombination events were retained for analysis revealed several important features about the mammalian DSB repair process:The presence of the markers within the region of shared homology did not affect the efficiency of gene targeting. In the majority of recombinants, the vector-borne marker proximal to the DSB was absent, being replaced with the corresponding chromosomal restriction enzyme site. This result is consistent with either formation and repair of a vector-borne gap or an "end" bias in mismatch repair of heteroduplex DNA (hDNA) that favored the chromosomal sequence. Formation of hDNA was frequently associated with gene targeting and, in most cases, began approximately 645 bp from the DSB and could encompass a distance of at least 1469 bp. The hDNA was efficiently repaired prior to DNA replication. The repair of adjacent mismatches in hDNA occurred predominantly on the same strand, suggesting the involvement of a long-patch repair mechanism.  (+info)

The molecular basis of multiple vector insertion by gene targeting in mammalian cells. (3/2670)

Gene targeting using sequence insertion vectors generally results in integration of one copy of the targeting vector generating a tandem duplication of the cognate chromosomal region of homology. However, occasionally the target locus is found to contain >1 copy of the integrated vector. The mechanism by which the latter recombinants arise is not known. In the present study, we investigated the molecular basis by which multiple vectors become integrated at the chromosomal immunoglobulin mu locus in a murine hybridoma. To accomplish this, specially designed insertion vectors were constructed that included six diagnostic restriction enzyme markers in the Cmu region of homology to the target chromosomal mu locus. This enabled contributions by the vector-borne and chromosomal Cmu sequences at the recombinant locus to be ascertained. Targeted recombinants were isolated and analyzed to determine the number of vector copies integrated at the chromosomal immunoglobulin mu locus. Targeted recombinants identified as bearing >1 copy of the integrated vector resulted from a Cmu triplication formed by two vector copies in tandem. Examination of the fate of the Cmu region markers suggested that this class of recombinant was generated predominantly, if not exclusively, by two targeted vector integration events, each involving insertion of a single copy of the vector. Both vector insertion events into the chromosomal mu locus were consistent with the double-strand-break repair mechanism of homologous recombination. We interpret our results, taken together, to mean that a proportion of recipient cells is in a predetermined state that is amenable to targeted but not random vector integration.  (+info)

Infrequent translation of a nonsense codon is sufficient to decrease mRNA level. (4/2670)

In many organisms nonsense mutations decrease the level of mRNA. In the case of mammalian cells, it is still controversial whether translation is required for this nonsense-mediated RNA decrease (NMD). Although previous analyzes have shown that conditions that impede translation termination at nonsense codons also prevent NMD, the residual level of termination was unknown in these experiments. Moreover, the conditions used to impede termination might also have interfered with NMD in other ways. Because of these uncertainties, we have tested the effects of limiting translation of a nonsense codon in a different way, using two mutations in the immunoglobulin mu heavy chain gene. For this purpose we exploited an exceptional nonsense mutation at codon 3, which efficiently terminates translation but nonetheless maintains a high level of mu mRNA. We have shown 1) that translation of Ter462 in the double mutant occurs at only approximately 4% the normal frequency, and 2) that Ter462 in cis with Ter3 can induce NMD. That is, translation of Ter462 at this low (4%) frequency is sufficient to induce NMD.  (+info)

Soluble class I MHC with beta2-microglobulin covalently linked peptides: specific binding to a T cell hybridoma. (5/2670)

Soluble forms of the mouse MHC class I molecule, Dd, were produced in which the peptide binding groove was uniformly occupied by peptides attached via a covalent flexible peptide linker to the N terminus of the associated beta2-microglobulin. The MHC heavy chain and beta2-microglobulin were firmly associated, and the molecules displayed an Ab epitope requiring proper occupancy of the peptide binding groove. Soluble Dd containing a covalent version of a well-characterized Dd-binding peptide from HIV stimulated a T cell hybridoma specific for this combination. Furthermore, a tetravalent version of this molecule bound specifically with apparent high avidity to this hybridoma.  (+info)

Cloning, expression, and characterization of the Fab fragment of the anti-lysozyme antibody HyHEL-5. (6/2670)

Hybridoma cDNAs encoding the individual chains of the Fab fragment of the well characterized murine monoclonal antibody HyHEL-5 were cloned and sequenced. The recombinant Fab fragment was produced by expressing each chain in a separate Escherichia coli pET vector, denaturing inclusion bodies and co-refolding. Characterization of the purified Fab by MALDI-TOF mass spectrometry and N-terminal amino acid sequencing demonstrated proper processing of the individual chains. The association of the recombinant Fab fragment with hen egg lysozyme and the avian epitope variant bobwhite quail lysozyme was found by isothermal titration calorimetry to have energetics very similar to that of the HyHEL-5 IgG. Heterologous expression of the HyHEL-5 Fab fragment opens the way to structure/function studies in this well-known system.  (+info)

A novel 62-kilodalton egg antigen from Schistosoma mansoni induces a potent CD4(+) T helper cell response in the C57BL/6 mouse. (7/2670)

In infection with Schistosoma mansoni, hepatic granuloma formation is mediated by CD4(+) T helper (Th) cells sensitized to schistosomal egg antigens. There is considerable variation among infected individuals with respect to both severity of disease and the T-cell response to egg antigens. In the BL/6 mouse, the egg granulomas are relatively small and the relevant sensitizing egg antigens are largely unknown. We investigated the CD4(+) Th cell response of infected BL/6 mice to egg antigens fractionated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and found a prominent lymphoproliferative response to be directed against a 62-kDa component. With the aid of a specific T-cell hybridoma, 4E6, the 62-kDa antigen was isolated; following partial digestion with endoproteinase Glu-C, an internal amino acid sequence was found to be identical with one present in the enzyme phosphoenolpyruvate carboxykinase (PEPCK) of the organisms Caenorhabditis elegans and Treponema pallidum and to differ by one residue from PEPCK of various other species. In CD4(+) Th cells from 7.5- 8.5-week-infected BL/6 mice, the purified 62-kDa molecule elicited a potent proliferative response which, based on cytokine analysis, was of a mixed Th-1 and Th-2 type. Our results reveal a novel egg antigen of particular prominence in the BL/6 mouse and suggest that the immune response in schistosomiasis is a product of sensitization to egg antigens that may vary considerably in immunogenicity from strain to strain.  (+info)

Immunosuppressant deoxyspergualin-induced inhibition of cell proliferation is accompanied with an enhanced reduction of tetrazolium salt. (8/2670)

Deoxyspergualin (DSG) has both antitumor and immunosuppressive activities. We explored the mechanism of DSG activities using an aqueous soluble analogue, methyldeoxyspergualin (MeDSG) for in vitro culture studies. It is known that DSG has inhibitory effects on cell proliferation, and we also observed that MeDSG inhibited [3H]-thymidine incorporation by rapidly dividing murine T cell hybridomas. However, when tetrazolium (MTT) colorimetric assay was adopted to evaluate its inhibitory effects on cell proliferation, MeDSG induced an enhanced MTT reduction. When we examined whether these results were applicable to the actively dividing cells of other origins than T cells, similar effects were seen with Raji cells, J774.1 cells and NIH3T3 cells. N-30, another analogue which was capable of suppressing anti-SRBC antibody production in vivo, also induced inhibition of cell growth and an enhanced MTT reduction. In contrast, the analogue which failed to prevent the antibody production, neither enhanced MTT reduction nor inhibited cell proliferation. Our results demonstrated that the ability to generate MTT formazan in dividing cells is a common property among, DSG analogue with the immunosuppressive and antiproliferative activities.  (+info)

Use of I region-restricted, antigen-specific T cell hybridomas to produce idiotypically specific anti-receptor antibodies. |...Use of I region-restricted, antigen-specific T cell hybridomas to produce idiotypically specific anti-receptor antibodies. |...
Murine T cell hybridomas bearing receptors for antigen plus I region gene products were used as immunogens in mice in an effort to raise anti-receptor antisera. The antisera were assayed for anti-receptor activity by the ability to inhibit interleukin 2 production by the T cell hybridomas stimulated by antigen and I region expressing antigen-presenting cells. The T cell hybridomas used in these experiments were made by fusing antigen-specific, I region-restricted BALB/c T cell blasts to the AKR thymoma, BW5147. Three groups of mice were immunized with the T cell hybridomas: (BALB/c X AKR)F1 animals, syngeneic to the hybridoma; (BALB.B X aKR)F1 animals, differing from the hybridomas at H2; and (C.B20 X AKR)F1 animals, differing from the hybridomas at Igh. Mice were immunized multiple times and sera from individual animals were assayed for anti-receptor antibodies. In all groups, some mice produced anti-receptor antibodies by the criterion that they were inhibitory in the assay mentioned above. ...
http://www.jimmunol.org/content/130/3/1033.long
Jens Christian Jensenius - Research outputs - Research - Aarhus UniversityJens Christian Jensenius - Research outputs - Research - Aarhus University
Mannose-binding lectin (MBL) substitution: recovery of opsonic function in vivo lags behind MBL serum levels. / Brouwer, Nannette; Frakking, Florine N J; van de Wetering, Marianne D; van Houdt, Michel; Hart, Margreet; Budde, Ilona Kleine; Strengers, Paul F W; Laursen, Inga; Houen, Gunnar; Roos, Dirk; Jensenius, Jens C; Caron, Huib N; Dolman, Koert M; Kuijpers, Taco W.. In: Journal of Immunology, Vol. 183, No. 5, 2009, p. 3496-504.. Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaper › Journal article › Research › peer-review ...
https://pure.au.dk/portal/en/persons/jens-christian-jensenius
Pharmaceuticals | Free Full-Text | Hybridoma-Derived Idiotype Vaccine for Lymphoma: Approval Must Wait | NotesPharmaceuticals | Free Full-Text | Hybridoma-Derived Idiotype Vaccine for Lymphoma: Approval Must Wait | Notes
Hybridoma-derived idiotype vaccines have been used for the experimental treatment of human lymphoma over the last twenty years, providing evidence of biological efficacy, clinical efficacy and clinical benefit. However, the product that has come closer to regulatory approval is unlikely to clear that hurdle due to the insufficiently robust data obtained in a recently closed clinical trial. This review aims at discussing the reasons for hybridoma-derived idiotype vaccines, more difficult to produce but also more successful than recombinant idiotype vaccines so far, are unlikely to gain regulatory approval. In particular, it is necessary to examine the many peculiar features of this therapeutic approach in a broader context, with special attention to concepts like customized active immunotherapy and randomization. Most published trials based on hybridoma-derived idiotype vaccines are being analyzed, together with the yet non-peer reviewed data from the only randomized study conducted so far with this
http://www.mdpi.com/1424-8247/3/3/667/notes
Unscramble hybridomas | Words unscrambled from letters hybridomas | Scrabble Word hybridomas | Words Made with the Letters...Unscramble hybridomas | Words unscrambled from letters hybridomas | Scrabble Word hybridomas | Words Made with the Letters...
Unscramble hybridomas, Unscramble letters hybridomas, Point value for hybridomas, Word Decoder for hybridomas, Word generator using the letters hybridomas, Word Solver hybridomas, Possible Scrabble words with hybridomas, Anagram of hybridomas
http://www.allscrabblewords.com/word-description/hybridomas
Analysis of T-cell hybridomas with an unusual MHC class II-dependent ligand specificity<...
TY - JOUR. T1 - Analysis of T-cell hybridomas with an unusual MHC class II-dependent ligand specificity. AU - Mendiratta, S. K.. AU - Singh, Nagendra. AU - Bal, V.. AU - Rath, S.. PY - 1996/1/1. Y1 - 1996/1/1. N2 - We have characterized two unusual T-cell hybridomas, 1E3 and 3B8, from H-2(k) mice immunized with I-Ab-transfected L cells (H-2(k)), that are stimulated by L cells transfected with I-Ab, I-A(k) or I-Eb, but not by non-transfected L cells. These hybridomas could not be stimulated by spleen cells from H-2(i3), H-2(k), H-2b or H-2(d) mice. Monoclonal anti-I-A antibodies did not block their responses, suggesting that mouse major histocompatibility complex (MHC) class II molecules may be peptide donors rather than restriction elements for them. The stimulation of these hybridomas by fibroblast targets was not blocked by an anti-H-2k(k),D(k)-specific monoclonal antibody. Lipopolysaccharide (LPS)-activated splenic and peritoneal exudate cells from H-2(k), H-2(d), H-2(i3), H-2b as well as ...
https://augusta.pure.elsevier.com/en/publications/analysis-of-t-cell-hybridomas-with-an-unusual-mhc-class-ii-depend
Changes of monosaccharide availability of human hybridoma lead to alteration of biological properties of human monoclonal...
TY - JOUR. T1 - Changes of monosaccharide availability of human hybridoma lead to alteration of biological properties of human monoclonal antibody. AU - Tachibana, Hirofumi. AU - Taniguchi, Kiyotaka. AU - Ushio, Yoshitaka. AU - Teruya, Kiichiro. AU - Osada, Kazuhiro. AU - Murakami, Hiroki. PY - 1994/1/1. Y1 - 1994/1/1. N2 - The effect of glucose and other monosaccharide availability in culture medium on production of antibody by human hybridomas has been studied. Human hybridoma cells C5TN produce an anti lung cancer human monoclonal antibody, and the light chain is N-glycosylated at the variable region. When the cell line was grown in the presence of various concentrations of glucose, the antibodies produced changed their antigen-binding activities. Analysis of the light chains produced under these condition revealed that four molecular-mass variant light chains ranging from about 26 to 32 kDa were secreted. The twenty six-kDa species, which corresponds to a non-glycosylated form of the light ...
https://kyushu-u.pure.elsevier.com/ja/publications/changes-of-monosaccharide-availability-of-human-hybridoma-lead-to
Equivalence of human and mouse CD4 in enhancing antigen responses by a mouse class II-restricted T cell hybridoma. | Journal of...Equivalence of human and mouse CD4 in enhancing antigen responses by a mouse class II-restricted T cell hybridoma. | Journal of...
We have examined the ability of hCD4 to interact functionally with mouse class II MHC molecules using the mouse T cell hybridoma BI-141, specific for beef insulin. We have previously shown that expression of mouse CD4 results in a marked enhancement of IL-2 release by BI-141 cells in response to beef insulin or, in a cross-reactive response, to pork insulin, on the appropriate mouse APCs. We now demonstrate that expression of hCD4 results in an equivalent stimulation of antigen responses by this mouse T cell hybridoma. The specificity of this effect was demonstrated by mAb and gp120 blocking studies. These data provide the first direct evidence for function of hCD4 and in an exclusively mouse system. ...
https://rupress.org/jem/article/170/6/1879/50218/Equivalence-of-human-and-mouse-CD4-in-enhancing
A murine hybridoma with large cytoplasmic inclusions of kappa light chains. | JEMA murine hybridoma with large cytoplasmic inclusions of kappa light chains. | JEM
A murine hybridoma cell line has been established that consistently forms large cytoplasmic inclusions. These structures bind antibody specific for mouse kappa L chain when stained in situ. SDS-PAGE analysis of isolated inclusion bodies produce a single protein band of approximately 26,000 Mr that reacts with anti-kappa antibody when transferred to nitrocellulose. No carbohydrate was detected in association with the purified protein. These data are consistent with the intracellular retention and deposition of complete kappa L chain protein. ...
http://jem.rupress.org/content/165/2/578
Detection and characterization of murine ecotropic recombinant virus in myeloma and hybridoma cells<...Detection and characterization of murine ecotropic recombinant virus in myeloma and hybridoma cells<...
TY - JOUR. T1 - Detection and characterization of murine ecotropic recombinant virus in myeloma and hybridoma cells. AU - Deo, Y.. AU - Ghebremariam, H.. AU - Cloyd, M.. PY - 1994. Y1 - 1994. N2 - Ecotropic recombinant virus (ERV), a relatively new class of murine retrovirus endogenous to mice, is expressed at significant levels by most murine myeloma and hybridoma cells examined. The routine XC, S+L-, mink cell focus-inducing (MCF), and reverse transcriptase (RT) tests are not suitable to detect and quantify the levels of ERV. A serological focus assay, based on specific anti-murine leukemia virus (MuLV) viral envelope (env) antibodies, is required to detect ERV. A more sensitive format of this serological focus assay includes co-cultivation of test article cells with the indicator (Mus dunni) cells. ERV isolated from murine hybridoma cells show a unique pattern of cross-reactivity with anti-MuLV env antibodies and this pattern is clearly distinct from that of ectropic and xenotropic ...
https://researchexperts.utmb.edu/en/publications/detection-and-characterization-of-murine-ecotropic-recombinant-vi
Plus itPlus it
The bispecific monoclonal antibody (Bi-MAb) HRS-3/AP-1 was developed by somatic hybridization of the 2 mouse hybridoma cell lines HRS-3 and AP-1, which produce monoclonal antibodies with reactivity against the Hodgkins- and Reed-Sternberg cell-associated CD30 antigen and alkaline phosphatase, respectively. After an active incubation with alkaline phosphatase, purified whole immunoglobulin molecules and F(ab′)2 fragments of the Bi-MAb were equally effective in converting a relatively noncytotoxic prodrug, mitomycin phosphate (MOP), into mitomycin alcohol, which was 100 times more toxic to the Hodgkins- and Reed-Sternberg cell line L540 (CD30+) than MOP. The cytotoxic activity of MOP was unaffected when the cells were pretreated with either the Bi-MAb or the enzyme alone. The Bi-MAb HRS-3/AP-1 did not bind to HPB-ALL cells (CD30-) and was not able to activate MOP on these cells. In cocultivation experiments with HPB-ALL and L540 cells, the activation of MOP by the Bi-MAb HRS-3/AP-1 and ...
https://cancerres.aacrjournals.org/content/50/21/6944
Expression and function of the transfected CD8 alpha chain in murine T cell hybridomas - GeoScience.netExpression and function of the transfected CD8 alpha chain in murine T cell hybridomas - GeoScience.net
Kanagawa, O.; Nakauchi, H.; Sekaly, R.P.; Maeda, K.; Takagaki, Y., 1990: Expression and function of the transfected CD8 alpha chain in murine T cell hybridomas
https://geoscience.net/research/007/333/007333832.php
MHC-II antigen presentation by W cells is usually important in order | Ubiquitin-Mediated Proteolysis of the Neuronal and Tumor...MHC-II antigen presentation by W cells is usually important in order | Ubiquitin-Mediated Proteolysis of the Neuronal and Tumor...
MHC-II antigen presentation by W cells is usually important in order for W cells to receive ideal costimulation from helper Compact disc4+ T cells. blend partner was created in the 1970s to generate B-cell hybridomas that secrete monoclonal antibodies (Kohler and Milstein, 1975). Thereafter Shortly, this technique was used to Capital t cells to create T-cell hybridomas that secrete IL-2 after TCR signaling (Kappler et al., 1982; Rock and roll et al., 1990). In light of the useful advantages of using peptide-specific T-cell hybridomas, researchers possess broadly used them as a device to quantitatively measure peptide-specific antigen demonstration by multiple types of antigen showing cells (APC). Vidovic et al exhibited that the adhesion substances and integrins in human being and murine Capital t cells are extremely extremely functionally conserved and murine T-cell:human being APC conversation happened easily (Vidovic et al., 2003). We and others possess utilized HLA-DR transgenic rodents to ...
http://pyr-41.com/mhc-ii-antigen-presentation-by-w-cells-is-usually-important-in-order/
Opti-Clone II - Hybridoma Cloning Factor (0910010) - MP BiomedicalsOpti-Clone II - Hybridoma Cloning Factor (0910010) - MP Biomedicals
MPs Opti-Clone™ is a partially purified hybridoma growth medium supplement which improves the cloning efficiency of murine B-cell hybridomas. It will enhance the growth of hybridomas cultured at low cell densities, and it will dramatically increase the number of antibody producing colonies during HAT selection. It supports the growth of hybridomas used in the manufacture of monoclonal antibodies. The formulation is suitable for use in cloning and fusion applications. Features: ∙ Promotes hybridoma growth ∙ Eliminates feeder cell layers ∙ Increases antibody production ∙ Improves stressed cells viability ∙ Improves surviving hybridomas ...
http://www.mpbio.com/product.php?pid=0910010&country=223
Induction of c-ets and c-fos gene expression upon antigenic stimulation of a T cell hybridoma with inducible cytolytic capacity...Induction of c-ets and c-fos gene expression upon antigenic stimulation of a T cell hybridoma with inducible cytolytic capacity...
Expression of cellular oncogenes was studied in a T cell hybridoma that undergoes cytolytic activation when stimulated by specific antigen or by anti-Thy-1 antibody. The activation occurs without induction of hybridoma proliferation, providing a model to examine oncogene expression during functional differentiation of lymphocytes. We found that c-fos and c-ets-1 mRNAs were transiently induced at high levels in the hybridoma 30 min and 4 h after stimulation, respectively. c-myc and c-ets-2 oncogenes were constitutively expressed in the hybridoma and their mRNA levels were unaffected during 4 h of stimulation, although c-myc expression was reduced in the later stage of stimulation. Inhibitors of T cell activation, cyclosporin A and anti-LFA-1 antibody, blocked the induction of c-fos and c-ets-1 mRNAs without reducing the levels of c-myc and c-ets-2. The results indicate that the functional activation of the CTL hybridoma is associated with induction of c-fos and c-ets-1 genes. ...
http://jem.rupress.org/content/166/3/810
New heteromyeloma cell lines for the production of human monoclonal antibodies. | OPENBS Open Archive UniBSNew heteromyeloma cell lines for the production of human monoclonal antibodies. | OPENBS Open Archive UniBS
The aim of this study was to establish hybridomas capable of long-term production of human monoclonal antibodies (mAbs). Heterohybridization was performed between the mouse myeloma cell line P3X63Ag8.653 and activated human peripheral blood lymphocytes (PBL). In order to achieve better retention of human chromosomes, as well as to improve the stability of the heterohybrids, one HAT-sensitive immunoglobulin (Ig)-non-secreting human x mouse (h x m) heteromyeloma was fused for a second time with activated human PBL. In this way, a panel of HAT-sensitive Ig-non-secreting h x h x m heteromyelomas was obtained and tested for its ability to generate stable human Ig-secreting heterohybrids with activated human PBL. Six lines were selected on the basis of their enhanced characteristics of fusion efficiency and genetic stability. When fused with in vitro immunized human PBL, they generated several h x h x h x m hybridomas stably secreting high yields (10-23 micrograms/ml/24 h) of human mAbs reactive with ...
https://iris.unibs.it/handle/11379/6833
Transmural pressure induces IL-6 secretion by intestinal epithelial cells<...
TY - JOUR. T1 - Transmural pressure induces IL-6 secretion by intestinal epithelial cells. AU - Kishikawa, H.. AU - Miura, S.. AU - Yoshida, H.. AU - Hirokawa, M.. AU - Nakamizo, H.. AU - Higuchi, H.. AU - Adachi, M.. AU - Nakatsumi, R. C.. AU - Suzuki, H.. AU - Saito, H.. AU - Ishii, H.. N1 - Copyright: Copyright 2008 Elsevier B.V., All rights reserved.. PY - 2002. Y1 - 2002. N2 - Although intestinal epithelial cells (IECs) are known as an important source for IL-6, it is not known whether mechanical forces affect IL-6 production. We investigated how transmural pressure modulates IL-6 synthesis and activation of transcription factors in IECs. Pressure was loaded onto IEC-18 cells by introducing compressed helium gas into the cell culture flask for 1-48 h. IL-6 release into the culture media was determined by cell proliferation bioassay using an IL-6-dependent mouse hybridoma cell line (7TD1). Exposure to pressure (80mmHg) significantly enhanced IL-6 release into the culture media from IEC-18 ...
https://keio.pure.elsevier.com/en/publications/transmural-pressure-induces-il-6-secretion-by-intestinal-epitheli
Primed B cells present type-II collagen to T cells.Primed B cells present type-II collagen to T cells.
article{9a5c9918-f28d-42f7-9e4a-e617d47af020, abstract = {Development of type-II collagen (CII)-induced arthritis (CIA) is dependent on a T-cell mediated activation of autoreactive B cells. However, it is still unclear if B cells can present CII to T cells. To investigate the role of B cells as antigen-presenting cells (APCs) for CII, we purified B cells from lymph nodes of immunized and nonimmunized mice. These B cells were used as APC for antigen-specific T-cell hybridomas. B cells from naïve mice did present native, triple-helical, CII (nCII) but also ovalbumin (OVA) and denatured CII (dCII) to antigen-specific T-cell hybridomas. In addition, B cells primed with nCII or OVA, but not dCII, activated the antigen-specific T-cell hybridomas two to three times better than naïve B cells. We conclude that antigen-primed B cells have the capacity to process and present CII to primed T cells, and antigen-primed antigen-specific B cells are more efficient as APC than naïve B cells. We further ...
https://lup.lub.lu.se/search/publication/107767
Technology / Software - Hybridoma cell lines secreting monoclonal antibodies (Ref. TEC0072) - Content - Knowledge Portal (UPF)Technology / Software - Hybridoma cell lines secreting monoclonal antibodies (Ref. TEC0072) - Content - Knowledge Portal (UPF)
Hybridomas are obtained by fusing normal B lymphocytes with a myeloma (tumor B cell line). Thus, a cloned hybridoma cell line has an unlimited capacity to grow in culture, producing...
https://www.upf.edu/web/knowledge/-/technology-software-hybridoma-cell-lines-secreting-monoclonal-antibodies-ref-tec0072-
XP Media and CloneMedia kits for Mouse Hybridoma Generation | Molecular devicesXP Media and CloneMedia kits for Mouse Hybridoma Generation | Molecular devices
Read PDF to know how XP Media & CloneMedia for Mouse Hybridoma Generation provide a complete solution that supports all stages of hybridoma cell line development.
https://www.moleculardevices.com/en/assets/app-note/reagents/xp-media-clonemedia-mouse-hybridoma-generation
False-col SEM of hybridoma cell - Stock Image G400/0031 - Science Photo LibraryFalse-col SEM of hybridoma cell - Stock Image G400/0031 - Science Photo Library
False-colour scanning electron micrograph of a hybridoma cell producing a monoclonal antibody to cytoskeleton protein. Monoclonal antibodies are produced by injecting a mouse with an antigen & harvesting its antibody response by removing the B lymphocytes. These are fused with myeloma (tumour) cells taken from a mutant lymphocyte source. The fusion product is called a hybridoma cell. B lymphocytes are short-lived; when fused with tumour cells, which divide indefinitely, the continuous production of antibody is secured. Hybridomas are screened for the required antibody; they are then cloned & produce that antibody. Magnification: x1500 at 35mm size. - Stock Image G400/0031
http://www.sciencephoto.com/media/214220/view
HybridomasHybridomas
Hybridomas are immortalized cells derived from the fusion of B lymphoblasts with a myeloma fusion partner. Some hybridomas in the ATCC collection are somatic cell hybrids. These cells are capable of producing immunoglobulins that are specific for viral, bacterial or cellular targets.
https://www.atcc.org/Products/Cells_and_Microorganisms/Hybridomas.aspx?slp=1
Adaltis S.r.l. - Hybridomas (Cell Lines) & Monoclonal Antibody ProductionAdaltis S.r.l. - Hybridomas (Cell Lines) & Monoclonal Antibody Production
Adaltis can produce purified and not purified antibody by using its cell lines. Antibodies can be purified on request by: HPLC , FPLC , affinity chromatography and gel filtration.. For production service information please contact: [email protected] ...
http://www.adaltis.net/services/hybridomas-cell-lines-monoclonal-antibody-production/
COG3 - DNA Damage Signalling and Repair InhibitorsCOG3 - DNA Damage Signalling and Repair Inhibitors
The variable-region genes of monoclonal antibody against spores were cloned from mouse hybridoma cells by reverse transcription-PCR. food spoilage (9). Control of the bacterial spores in meals processing is vital that you ensure the basic safety and an extended shelf lifestyle of foods. To keep the product quality and basic safety of foods, polyclonal and […]. ...
http://ranscombehouseglynde.com/tag/cog3/
Patent US6391637 - Use of CD40 ligand, a cytokine that binds CD40, to stimulate hybridoma cells - Google PatentsPatent US6391637 - Use of CD40 ligand, a cytokine that binds CD40, to stimulate hybridoma cells - Google Patents
There is disclosed a polypeptide (CD40-L) and DNA sequences, vectors and transformed host cells useful in providing CD40-L polypeptides. More particularly, this invention provides isolated human and murine CD40-L polypeptides that bind to the extracellular binding region of a CD40 receptor. Also disclosed are methods of simulating hybridoma cells to increase monoclonal antibody production by administering a CD40 ligand polypeptide that stimulates B cell proliferation.
http://www.google.com.au/patents/US6391637
METHODS OF PRODUCING HYBRIDOMAS AND MONOCLONAL ANTIBODIES AND ANTIBODIES PRODUCED THEREBY - diagram, schematic, and image...METHODS OF PRODUCING HYBRIDOMAS AND MONOCLONAL ANTIBODIES AND ANTIBODIES PRODUCED THEREBY - diagram, schematic, and image...
METHODS OF PRODUCING HYBRIDOMAS AND MONOCLONAL ANTIBODIES AND ANTIBODIES PRODUCED THEREBY - diagram, schematic, and image 150 ...
http://www.patentsencyclopedia.com/imgfull/20130018176_150
Patent US5700466 - Method of ameliorating or preventing septic shock using a monoclonal ... - Google PatentsPatent US5700466 - Method of ameliorating or preventing septic shock using a monoclonal ... - Google Patents
The present invention pertains to the novel hybridoma SDW18.1.1, hybridomas obtained from SDW18.1.1, monoclonal antibodies obtained from such hybridomas and derivatives of such monoclonal antibodies. The novel hybridomas are formed by fusion of cells from a mouse myeloma line and spleen cells from a mouse previously immunized with cachectin/TNF. Diagnostic and therapeutic utilities for the monoclonal antibodies and their derivatives are proposed, and testing procedures, materials in kit form and pharmaceutical compositions are likewise set forth.
http://www.google.com.au/patents/US5700466
Anti-ABeta Globulomer Antibodies, Antigen-Binding Moieties Thereof, Corresponding Hybridomas, Nucleic Acids, Vectors, Host...Anti-ABeta Globulomer Antibodies, Antigen-Binding Moieties Thereof, Corresponding Hybridomas, Nucleic Acids, Vectors, Host...
Anti-ABeta Globulomer Antibodies, Antigen-Binding Moieties Thereof, Corresponding Hybridomas, Nucleic Acids, Vectors, Host Cells, Methods of Producing Said Antibodies, Compositions Comprising Said Antibodies, Uses Of Said Antibodies And Methods Of Using Said Antibodies - diagram, schematic, and image 83 ...
http://www.patentsencyclopedia.com/imgfull/20090191190_83
DO11.10 TCR Antibody, anti-mouse - Hybridomas - MACS Antibodies - Products - Miltenyi Biotec - Danmark
The DO11.10 mouse model is a valuable tool for studies of T cell immigration, immunoregulation, development, activation, and function. The KJ1-26 monoclonal antibody reacts with the T cell receptor (TCR) expressed on lymphocytes of the DO11.10 transgenic mouse and the TCR of the BALB/c-derived DO11.10 and DO11.10.24 T cell hybridoma. The DO11.10 TCR is specific for the chicken ovalbumin (OVA) peptide (323-339) in the context of I-A[d] major histocompatibility (MHC) molecules. Transgenic DO11.10 T cells also recognize OVA peptide from jungle fowl and turkey in the presence of A20-1.11, the H-2d-bearing, antigen-presenting B cell lymphoma. - Danmark
https://www.miltenyibiotec.com/DK-en/products/do11-10-tcr-antibody-anti-mouse-kj1-26.html
Fibroblast bluesFibroblast blues
In a previous article, RAMPITSCH at BCRSSU.AGR.CA wrote: ,Hello nets: ,Im not sure if this is the right place to ask, but ..... ,I have a hybridoma cell line contaminated with fibroblasts. Normally I dont ,have a problem eliminating these since they stick to the plate a lot better ,than the hybridomas do: simply transferring the cells to a new plate usually ,does the trick. Now I have a line of fibroblasts which refuses to die (even ,after two months and more transfers than I care to remember). The hybridoma ,cell line (needless to say an important one) is growing poorly as it is barely ,able to attach to the plate and cant compete with these aggressive fibro- ,blasts (maybe they have been transformed?). Is there a miracle anti-fibro- ,blast agent? Is there any way of selectively getting rid of these fibroblasts? , ,Thanks for any help and comments, ,Chris R. , Chris: I may be brain-cramping but cant you just sort out the hybridomas? Or even pan them? Use something like an anti-Ig-FITC ...
http://www.bio.net/bionet/mm/immuno/1993-July/000454.html
Regulation of hybridoma antibody production by coculture with antigen by J J. Gibbons, Y T. Kim et al.Regulation of hybridoma antibody production by coculture with antigen by J J. Gibbons, Y T. Kim et al.
Gibbons, J J.; Kim, Y T.; and Siskind, G W., Regulation of hybridoma antibody production by coculture with antigen specific or idiotype specific immune spleen cell. Abstr. (1982). Subject Strain Bibliography 1982. 1805 ...
https://mouseion.jax.org/ssbb1982/1805/
Kinetic characterization of metabolism and antibody production of a hybridoma cell line in high density homogeneous perfusion...Kinetic characterization of metabolism and antibody production of a hybridoma cell line in high density homogeneous perfusion...
All rights reserved. No part of this book may be reproduced in any form by any electronic or mechanical means without permission in writing from the author ...
http://libarchive.dartmouth.edu/cdm/compoundobject/collection/dcdis/id/150415/rec/45
Growth limitation in hybridoma cell cultures: The role of inhibitory or toxic metabolites | SpringerLinkGrowth limitation in hybridoma cell cultures: The role of inhibitory or toxic metabolites | SpringerLink
Hybridoma cells usually grow to fairly low cell densities in batch cultures (1-3×106 cells/ml). The reason for this is either that essential nutritional components of the medium are consumed, or that
https://link.springer.com/article/10.1007%2FBF00135634
KAKEN - Research Projects | INDUCTION OF HUMAN HEPATOMA-SPECIFIC IMMUNE RESPONSE BY HEPATOMA-DENDRITIC CELL HYBRIDOMAS (KAKENHI...KAKEN - Research Projects | INDUCTION OF HUMAN HEPATOMA-SPECIFIC IMMUNE RESPONSE BY HEPATOMA-DENDRITIC CELL HYBRIDOMAS (KAKENHI...
Principal Investigator：SHIMIZU Yukihiro, Project Period (FY)：1999 - 2000, Research Category：Grant-in-Aid for Scientific Research (C), Section：一般, Research Field：Gastroenterology
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-11670485/
Institut für Softwaretechnik und Theoretische Informatik: Multimodal InteractionInstitut für Softwaretechnik und Theoretische Informatik: Multimodal Interaction
Topics In this course we will set the basics for an understanding of multimodal communication between humans and multimodal interaction between humans and machines. We will start with clarifying the basic principles of human-human communication and human-machine interaction. We will then describe the processes taking place in humans when perceiving auditory, visual and tactile signals, as well as how these perceptions are integrated in order to form a multimodal perception. The signals can be generated and received by machines which are able to interact with humans in limited domains. The set-up of such machines will be discussed, and limitations as well as potential solutions to overcome these limitations will be explained. The course is held in the MOOC format. Only the first session will take place in the lecture hall, where further information will be given and registration takes place. Being present in this session is mandatory for participating in the course. The remainder of the course ...
https://www.qu.tu-berlin.de/menue/studium_und_lehre/vergangene_semester/lehrangebot_ss_2019/multimodal_interaction/parameter/maxhilfe/mobil/
Institut für Softwaretechnik und Theoretische Informatik: Multimodal InteractionInstitut für Softwaretechnik und Theoretische Informatik: Multimodal Interaction
Language: English VL Time: MOOC; New lecture available every week. VL Room: Online ISIS course: https://isis.tu-berlin.de/course/view.php?id=23345 Intro to the course and Q&A session in Zoom on April 19th, 2021 at 12:00-14:00. The link will be provided in the ISIS course. Please have a look into the intro video of the the course in ISIS and the structure of the course before the online session on April 19th. During this Zoom session we will introduce the general plan of the course and you can ask your questions about it. From 26th of April the lectures with chapters will be unlocked weekly, with homework and/or project tasks for each chapter. Topics In this course we will set the basics for an understanding of multimodal communication between humans and multimodal interaction between humans and machines. We will start with clarifying the basic principles of human-human communication and human-machine interaction. We will then describe the processes taking place in humans when perceiving ...
https://www.qu.tu-berlin.de/menue/studium_und_lehre/lehrangebot_ss_2021/multimodal_interaction/parameter/de/font2/maxhilfe/
Corona Virus Disease (COVID-19)Corona Virus Disease (COVID-19)
1. It is the disease caused by the novel coronavirus. Patients may have fever with respiratory symptoms - cough, runny nose, sore throat or difficulty breathing. It can even be fatal if the symptoms are severe.. 2. Route of Transmission: Human-Human transmission through droplets from coughing and sneezing of infected person and close contact with COVID-19 cases.. 3. Incubation period: 2-14 days. ...
https://ddc.moph.go.th/viralpneumonia/eng/ind_knowledge.php
News | KurzweilAINews | KurzweilAI
An international team has developed an AI algorithm with social skills that has outperformed humans in the ability to cooperate with people and machines in playing a variety of two-player games. The researchers, led by Iyad Rahwan, PhD, an MIT Associate Professor of Media Arts and Sciences, tested humans and the algorithm, called S# (S sharp), in three types of interactions: machine-machine, human-machine, and human-human. In most instances, machines programmed with… read more. ...
http://www.kurzweilai.net/news?main=/news/news_single.html%3Fid%253D11237
News | KurzweilAINews | KurzweilAI
An international team has developed an AI algorithm with social skills that has outperformed humans in the ability to cooperate with people and machines in playing a variety of two-player games. The researchers, led by Iyad Rahwan, PhD, an MIT Associate Professor of Media Arts and Sciences, tested humans and the algorithm, called S# (S sharp), in three types of interactions: machine-machine, human-machine, and human-human. In most instances, machines programmed with… read more. ...
http://www.kurzweilai.net/news?main=/news/news.html%3Fcat%253D14%2526d%253D0
Join us for: Plantarium: Whats up at the Hub: Tema Genus: TEMA - Institutionen för Tema
The Gothenburg-based artist Nino Mick suggests in one of their poems that ultimately biology is our only home. The film programme suggests different ways of approaching such an understanding of biology as home. This film programme consists of a screening of two movies: Pojktanten and a short film/installation, Sporing Lips of Transposed Desire. Each of these two audio-visual artefacts explore floral-aesthetics through something that can be rendered as plant-human intimacies. The programme is inviting the audience as entrants - more than simply viewers - to engage in these ecological intimacies and to think of the ethics and potentials of thinking intimacies as beyond a human-human relationality. Approaching the films through such an angle of ecological intimacy might allow to perceive a decentred understanding of the human through multiplicities and entanglements with others. Wibke will offer in a short introductory talk to the programme as a thinking tool to apply to the film ...
http://www.tema.liu.se/tema-g/Posthuman/arkiv-av-kalendarium/call-for-participants-for-a-workshop-plantarium?l=sv
Clinical Trials Registry | Internet Stroke Center
During the therapy session, visual 3D motion tracking will be used via Kinect, audio will be recorded, and basic measurements (i.e. the distance between a participants wrist and elbow) will be taken. After the patient-therapist interaction has been mapped, members of the research team will build computer based models of the therapist and patient using the insights gained from the human-human interaction. The models developed in this proposed study will be used as a template for programming safe and intuitive humanoid-patient interactions for future study. The model of the therapist will be implemented on the Robot (w/o a patient involved) and in a simulation environment where it will be tested with a computer-based model of the patient. ...
http://www.strokecenter.org/trials/clinicalstudies/robot-assisted-stroke-rehabilitation-based-on-patient-therapist-interactions/description
mouse anti-estrogen receptor alpha, ligand binding domain (aa 304-554) hybridoma (ERalpha BZ1) at eagle-i Network Shared...mouse anti-estrogen receptor alpha, ligand binding domain (aa 304-554) hybridoma (ERalpha BZ1) at eagle-i Network Shared...
mouse anti-estrogen receptor alpha, ligand binding domain (aa 304-554) hybridoma (ERalpha BZ1) is an eagle-i resource of type Hybridoma cell line at eagle-i Network Shared Resource Repository.
http://shared.eagle-i.net/i/00000140-d7b2-23d7-fa61-b3d480000000
mouse anti-late bloomer hybridoma (10C9 anti-late bloomer) at eagle-i Network Shared Resource Repositorymouse anti-late bloomer hybridoma (10C9 anti-late bloomer) at eagle-i Network Shared Resource Repository
mouse anti-late bloomer hybridoma (10C9 anti-late bloomer) is an eagle-i resource of type Hybridoma cell line at eagle-i Network Shared Resource Repository.
http://shared.eagle-i.net/i/00000140-d7d6-e7d9-fa61-b3d480000000
Analysis of suppressor T cells induced by donor-specific transfusion (DST): establishment of a human T cell hybridoma producing...
TY - JOUR. T1 - Analysis of suppressor T cells induced by donor-specific transfusion (DST). T2 - establishment of a human T cell hybridoma producing an antigen-nonspecific suppressor factor.. AU - Fujiwara, T.. AU - Sakagami, K.. AU - Kusaka, S.. AU - Uda, M.. AU - Orita, K.. PY - 1992. Y1 - 1992. N2 - Formation of suppressor T cells (Ts) induced by donor-specific transfusion (DST) is one of the most commonly suggested mechanisms for the beneficial effect of DST. In this study, we established a human T cell hybridoma derived from the peripheral blood lymphocytes (PBL) of a DST-treated patient, which produced an antigen-nonspecific suppressor factor. Post-DST PBL were fused with an azaguanine-resistant mutant of a human T cell leukemia cell line, CCRF-CEM(AG). After selection and cloning, we established one clone producing the mixed lymphocyte reaction (MLR) inhibitory factor (C524: 18%-43% suppression). Suppressive activity of the supernatant obtained from C524 after activation by PHA was highly ...
https://okayama.pure.elsevier.com/en/publications/analysis-of-suppressor-t-cells-induced-by-donor-specific-transfus
Production of Monoclonal Antibody by Hybridoma Technology Introduction - Free Dissertation Writing For StudentsProduction of Monoclonal Antibody by Hybridoma Technology Introduction - Free Dissertation Writing For Students
Cesar Milstein and Georges J.F. Kohler invented the production of monoclonal antibodies in 1975 for which they got the Noble Prize of 1984 for Medicine and Physiology along with Niels Kaj Jerne, who made other contributions to immunology. The term hybridoma was introduced by Leonard Herzenberg in 1976-1977. Principle: The method starts by injecting a mice with immunogen. The mice should response to immunogenic reaction. A kind of somatic cell, the B-cell, produces antibodies that bind to the immunogen. These recently created antibodies are then collected from the mice. These isolated cells are merged with immortal B-cells (a myeloma cell), in order to provide a hybrid cell known as Hybridoma. These hybridomas have the antibody producing ability of the B-cell and also have the reproducibility and longevity. The hybridomas are fully grown in culture medium. In addition, the manufactured antibodies are all chemically identical in distinct to polyclonal antibody.. Hybridoma Production of Monoclonal ...
https://artandspirituality.net/production-of-monoclonal-antibody-by-hybridoma-technology-introduction/
Guaranteed Mouse Monoclonal Antibody Production Services - Syd LabsGuaranteed Mouse Monoclonal Antibody Production Services - Syd Labs
The most comprehensive custom mouse monoclonal antibody production services: custom production of monoclonal antibodies against non-modified, phospho-specific, methylation-specific, and acetylation-specific peptides and proteins; monoclonal antibodies for ELISA, Western blot, IP, IF, ICC, and IHC applications. Guaranteed ELISA > 1:40 000.
http://www.sydlabs.com/guaranteed-mouse-monoclonal-antibody-production-services-p4.htm
Monoclonal antibodies to various morphologic components of human skin<...
TY - JOUR. T1 - Monoclonal antibodies to various morphologic components of human skin. AU - Aiba, S.. AU - Masuko, T.. AU - Hosokawa, M.. AU - Hashimoto, Y.. PY - 1983/1/1. Y1 - 1983/1/1. N2 - Somatic cell hybrids were established from the mouse myeloma, P3x63Ag8.653 cells, and the spleen cells of a mouse hyperimmune to human epidermal cells. Indirect immunofluorescence test with hybridoma culture fluids displayed that 253 out of 263 hybridoma cultures secreted antibodies reactive with the frozen sections of human skin. The hybridomas secreting unique antibodies to skin components were cloned and designated as AHS-1 to -8. Monoclonal antibodies (MoAb) from AHS-1, AHS-2, and AHS-3 hybridomas did detect cytoplasmic antigens present in the epidermal layer, eccrine ducts and glands (except MoAb AHS-1), and hair follicles. Enzyme-linked immunosorbent assay showed that the antigen recognized by either MoAb AHS-1 or MoAb AHS-2, but not by MoAb AHS-3, shares the antigenic determinant with antigen(s) ...
https://tohoku.pure.elsevier.com/en/publications/monoclonal-antibodies-to-various-morphologic-components-of-human-
HybriFree: a robust and rapid method for the development of monoclonal antibodies from different host species | BMC...HybriFree: a robust and rapid method for the development of monoclonal antibodies from different host species | BMC...
Monoclonal antibodies (MAbs) are made by identical immune cells and target one particular epitope by monovalent or monospecific affinity. The high affinity and selective binding of MAbs to epitopes in target antigens makes them highly potent tools for use in biochemistry, molecular biology and medicine. The first working method described for the isolation of monoclonal antibodies was hybridoma technology, based on forming hybrid cell lines (hybridomas) by fusing an antibody-producing B-cell with a myeloma cell [1]. The antibodies produced by a particular hybridoma clone share the same specificity. Thus, individual clones can be screened for the production of an antibody with the desired affinity. However, hybridoma technology has shortcomings: it takes a relatively long time (on the order of months) and has not been widely applied to organisms other than mice. Moreover, antibody sequence information is unavailable by this method. Thus, when a hybridoma-screened antibody is selected for further ...
https://bmcbiotechnol.biomedcentral.com/articles/10.1186/s12896-016-0232-6
Services Provided: Module 1 - NEI Vision Research CoreServices Provided: Module 1 - NEI Vision Research Core
Our hybridoma bank contains over 20 murine hybridomas. Moreover, we have extensive experience in the purification and characterization of numerous murine monoclonal antibodies for in vitro and in vivo use. We have also generated monoclonal antibodies de novo by in vivo immunization and subsequent in vitro fusion of heterologous fusion partners from Chinese hamsters and rats, and produced IgA-secreting hybridomas de novo. Depending on the quantity of monoclonal antibodies required, hybridomas are propagated in either static flat tissue culture flasks or in roller bottle cultures. Monoclonal antibodies are purified by affinity column chromatography using protein G charged columns. The affinity purified monoclonal antibodies are dialyzed against deionized water, concentrated on a vacuum concentrator, resuspended in sterile PBS, and the protein concentration determined by BCA. The purity of the monoclonal antibodies is confirmed by western blotting with commercial anti-mouse IgG or IgM, antibodies, ...
http://www.utsouthwestern.edu/labs/nei-vision/modules/module-1/services-provided.html
Monoclonal Antibody Production | MD Anderson Cancer CenterMonoclonal Antibody Production | MD Anderson Cancer Center
The MD Anderson Monoclonal Antibody Core Facility provides custom monoclonal antibody production and purification to researchers at MD Anderson and beyond. Learn more.
https://www.mdanderson.org/research/research-resources/core-facilities/monoclonal-antibody-core-facility.html
Caco-2 Vs Hybridoma cells - Cell BiologyCaco-2 Vs Hybridoma cells - Cell Biology
hi friends ! iam handling few cell lines ! we recently bought Caco-2 cell line. i have ended up mixing caco-2 cells with hybridoma cells and now iam unable to remove this contamination. Hybridoma cell line has a doubling time of just 24hrs is growing fast and soon will replace Caco-2 cells. caco-2 cells are really slow growing cells. i tried few mwthods to isolate them but failed ...
http://www.protocol-online.org/biology-forums/posts/15068.html
Commonalities Between pDCs and B Cells | Science SignalingCommonalities Between pDCs and B Cells | Science Signaling
Plasmacytoid dendritic cells (pDCs) are a subpopulation of dendritic cells that secrete large amounts of interferons (IFNs) in response to stimuli that activate the Toll-like receptors TLR9 or TLR7. The C-type lectin blood dendritic cell antigen 2 (BDCA2) is uniquely expressed by pDCs, and antibodies that bind BDCA2 inhibit IFN production in response to TLR signaling. Cao et al. found that the abundance of BDCA2 at the cell surface was increased, and signaling was reconstituted in Jurkat cells or a mouse T cell hybridoma cell line when the cells were also transfected to express the γ subunit of the Fcε receptor (FcεR1γ) but not when BDCA2 was coexpressed with the immunoreceptor tyrosine-based activation motif (ITAM) adaptor DAP12 or the related protein DAP10. Analysis of the mRNA and protein abundance of signaling proteins associated with either B cell receptor (BCR) or T cell receptor (TCR) signaling revealed that the signaling cascade of isolated human pDCs most resembled that downstream ...
http://stke.sciencemag.org/content/2007/411/tw404
Monoclonal Ab
Monoclonal Antibody Production Service (Taiwan) Start Order Immediately by Completing the Inquiry Form.. I. Hybridoma fusion and screening service: 16 weeks (USD 5,100). II. MAP Immunogen monoclonal antibody production: Total 19 weeks (USD 5,800). III. LAE antigen monoclonal antibody production: Total 20 weeks (USD 6,600). IV. Optional Services. I Hybridoma fusion and screening service: 16 weeks. ...
http://laebio.com/SvcmAb.asp
Monoclonal antibodies that immunoreact with a cation-stimulated plant membrane ATPase | Biochemical JournalMonoclonal antibodies that immunoreact with a cation-stimulated plant membrane ATPase | Biochemical Journal
Hybridoma technology has been used successfully to generate monoclonal-antibody probes against protoplast membrane antigens. Hybridomas secreting monoclonal antibodies that either inhibit or stimulate a putative plasma-membrane marker enzyme, (K+ + Mg2+)-stimulated pH 6.5 ATPase, have been identified and cloned. The specificity of monoclonal-antibody probes on the activity of other phosphate-hydrolysing enzymes has also been examined. The production and identification of monospecific antibodies capable of immunoreacting with particular component proteins in a complex plant membrane mixture highlight the usefulness of hybridoma methodology for the enzymologist, especially since such monoclonal antibodies can be used in the purification of proteins by immunoaffinity techniques. ...
http://www.biochemj.org/content/203/1/51
Modified hybridoma methodology: Antigen-directed chemically mediated cell fusion<...Modified hybridoma methodology: Antigen-directed chemically mediated cell fusion<...
TY - JOUR. T1 - Modified hybridoma methodology. T2 - Antigen-directed chemically mediated cell fusion. AU - Kranz, David M. AU - Herron, James N.. AU - Billing, Patricia A.. AU - Voss, Edward W.. PY - 1980/1/1. Y1 - 1980/1/1. N2 - The concept of antigen-directed cell fusions to increase the yield of hybridomas was investigated. To facilitate cell-cell contact, antigen conjugated cells were used in cell fusion studies. Specifically, fluorescyl conjugated murine myeloma cells (Sp 2/0-Ag14) incubated with murine immune (anti-fluorescyl) splenocytes formed aggregates containing fluorescent and non-fluorescent cells. Fusion of these populations with polyethylene glycol resulted in a greater number of anti-fluorescyl hybridomas relative to normal fusions under non-antigen directed condition. Ligand binding data indicated that despite the multicellular aggregates the hybridomas resulting from chemically mediated fusions produced only one monoclonal Ig.. AB - The concept of antigen-directed cell fusions ...
https://experts.illinois.edu/en/publications/modified-hybridoma-methodology-antigen-directed-chemically-mediat-2
Plus itPlus it
Within the functional group of transcriptional regulation, we find the transcription factor Fosl2 (also known as Fra2), a Fos family member, among the most prominently up-regulated by both chemopreventive compounds. Interestingly, an antitumor promoter, the phenolic antioxidant tert-butylhydroquinone, was reported to induce expression of Fra2 (Fosl2) as well as Fra1. Furthermore, the authors concluded that inhibitory activator protein-1 complexes composed of Jun-Fra heterodimers, induced by tert-butylhydroquinone, antagonize the transcriptional effects of the tumor promoter 12-O-tetradecanoylphorbol-13-acetate, which are mediated by Jun-Fos heterodimers (21). Similarly, inhibition of interleukin-6-stimulated cell growth of human myeloma and mouse hybridoma cells was shown to be associated with increased expression of Fra2 protein (22). Fra2 has also been associated with differentiation in epidermis, and exogenous expression of Fra-2 (Fosl2) repressed activator protein-1 transcriptional activity ...
http://cancerpreventionresearch.aacrjournals.org/content/2/2/175
Plus itPlus it
Recent isolates of RX54-3 hybridoma cells (new cells) protect BALB/c mice against subsequent challenge with the tumorigenic myeloma parent cells used to construct this hybridoma. In contrast, hybridoma cells which have been maintained in tissue culture for long periods of time (old cells) are not protective. In the present study, we compared a number of properties of the new and old hybridoma cells and determined which line was more similar to the parent myeloma. We found that new hybridoma cells resembled myeloma cells in: (a) possessing A- and C-type viral particles on transmission electron microscopy and a relatively smooth surface on scanning electron microscopy; (b) being sensitive to a hypotonic solution containing the dye propidium iodide; (c) having similar DNA histograms on flow cytometric analysis; (d) being sensitive to the bacteriocin colicin HSC 10; and (e) being tumorigenic in nude mice. In contrast, old hybridoma cells differed in all of these characteristics from new hybridoma ...
http://cancerres.aacrjournals.org/content/42/5/1904
Hybridomas and monoclonal antibodies : presented at the Thirty-Fourth Annual Meeting of the American Association of Blood Banks...Hybridomas and monoclonal antibodies : presented at the Thirty-Fourth Annual Meeting of the American Association of Blood Banks...
Get this from a library! Hybridomas and monoclonal antibodies : presented at the Thirty-Fourth Annual Meeting of the American Association of Blood Banks, November 3, 1981. [Serafeim P Masouredis;]
http://www.worldcat.org/title/hybridomas-and-monoclonal-antibodies-presented-at-the-thirty-fourth-annual-meeting-of-the-american-association-of-blood-banks-november-3-1981/oclc/12132275
Hybridoma culture using Eppendorf New Brunswick™ CelliGen® 310 with Packed-bed Fibra-Cel® Basket Impeller - Pharmaceutical...Hybridoma culture using Eppendorf New Brunswick™ CelliGen® 310 with Packed-bed Fibra-Cel® Basket Impeller - Pharmaceutical...
receive-email Wed like to keep you informed about relevant promotions, products and services, if you would like not to receive these, check this box.. ...
https://www.pharmaceutical-business-review.com/suppliers/eppendorf__cell__manipulation__liquid__handling/whitepapers/hybridoma-culture-using-eppendorf-new-brunswick-celligen-310-with-packed-bed-fibra-cel-basket-impeller/
Monoclonal Antibody Production - Sandwich ELISA guaranteed package - ProteoGenixMonoclonal Antibody Production - Sandwich ELISA guaranteed package - ProteoGenix
Premium package for production and purification of monoclonal antibodies guaranteed on sandwich ELISA. Discover also our list of available Packages online
https://www.proteogenix.science/antibody-production/monoclonal-antibody/premium-elisa-sandwich/
Monoclonal Antibody Production And PurificationMonoclonal Antibody Production And Purification
Production and Purification of your monoclonal antibodies (IgG, IgM and more) from Hybridoma or Cell Culture Supernatants produced by Davids or send to us.
https://www.davids-bio.com/pages/antibodies-monoclonal-production.html
Enhancement of hybridoma formation, clonability and cell proliferation in a nanoparticle-doped aqueous environment | BMC...Enhancement of hybridoma formation, clonability and cell proliferation in a nanoparticle-doped aqueous environment | BMC...
The isolation and production of human monoclonal antibodies is becoming an increasingly important pursuit as biopharmaceutical companies migrate their drug pipelines away from small organic molecules. As such, optimization of monoclonal antibody technologies is important, as this is becoming the new rate-limiting step for discovery and development of new pharmaceuticals. The major limitations of this system are the efficiency of isolating hybridoma clones, the process of stabilizing these clones and optimization of hybridoma cell secretion, especially for large-scale production. Many previous studies have demonstrated how perturbations in the aqueous environment can impact upon cell biology. In particular, radio frequency (RF) irradiation of solutions can have dramatic effects on behavior of solutions, cells and in particular membrane proteins, although this effect decays following removal of the RF. Recently, it was shown that nanoparticle doping of RF irradiated water (NPD water) produced a stabilized
https://bmcbiotechnol.biomedcentral.com/articles/10.1186/1472-6750-8-3/figures/4
Monoclonal Antibody Production - ELISA guaranteed package (Premium) - ProteoGenixMonoclonal Antibody Production - ELISA guaranteed package (Premium) - ProteoGenix
Premium package for production and purification of monoclonal antibodies guaranteed on ELISA system. Discover also our list of available Packages online
https://www.proteogenix.science/antibody-production/monoclonal-antibody/premium-elisa/
Epitopes of the cholera family of enterotoxinsEpitopes of the cholera family of enterotoxins
Hybridoma-derived monoclonal antibodies were raised to enterotoxins of the cholera family and to chimeric B-subunit proteins in which individual amino acid residues of a heat-labile, cholera-related enterotoxin from an Escherichia coli strain of porcine origin (P-LT) were substituted with correspond …
https://pubmed.ncbi.nlm.nih.gov/2440089/?dopt=Abstract
Altweb: Publications: Proceedings: Alternatives in Monoclonal Antibody Production
September 24-25, 1997 , Baltimore, MD. A workshop of The Johns Hopkins Center for Alternatives to Animal Testing and The Office for Protection from Research Risks, National Institutes of Health ...
http://altweb.jhsph.edu/pubs/proceedings/mab/
Monoclonal Antibody Production Service | Abbexa LtdMonoclonal Antibody Production Service | Abbexa Ltd
At Abbexa, we have exciting offers and news about our products and services that we want to share with you. You can select if you would like to be contacted about specific topics below. We will always treat your data with care. We never share your details with any other companies.. If you change your mind later, you can stop receiving our updates at any time by changing your contact preferences in your account, or letting us know by email or phone.. ...
https://www.abbexa.com/monoclonal-antibody-production-service
Generation of Anti-chemokine Monoclonal Antibodies with Migration Neutralizing Capacity: Their Potential as Therapeutic Agents...Generation of Anti-chemokine Monoclonal Antibodies with Migration Neutralizing Capacity: Their Potential as Therapeutic Agents...
Purpose: : Recruitment of immune cell subsets to the site of transplantation is potentiated in part by primordial chemokines such as CXCL1/KC and later CXCL9/Mig and CXCL10/IP-10, which are markedly up-regulated in high risk (HR) allogeneic corneal transplant recipients but not in syngeneic or normal risk allogeneic recipients. The purpose of this study is to address the effect of newly generated anti-chemokine mAbs as potential therapy for diminishing the influx of inflammatory immune cells into HR corneal allografts, therefore leading to enhanced graft survival. Methods: : Wild type (wt) C57BL/6 (B6), mice were immunized with CXCL1 peptide plus universal T cell epitope (PADRE) as adjuvant and boosted 14 days later with the same combination. Hybridoma lines were created by fusion of splenocytes from immunized mice with SP2/IL-6 myeloma cells. Anti-CXCL1/KC clones were selected based on their ability to bind whole CXCL1/KC by ELISA. Thioglycollate-elicited neutrophils or macrophages from ...
http://iovs.arvojournals.org/article.aspx?articleid=2353149
Hybridoma Hybridomas 4. Screening 5. Cloning and propagation - Free Critical Thinking For Students
An suitable adjuvant is blended with microgram or milligram amounts of immunogen and injected at multiple site of the mouse at various times in a repetitious manners. At times the mice is bled and examination for antibodies of purposed specificity is made. Highest concentration of antibodies are established after the mouse is immense with 2-3 individual delicate doses of antigen. At the point when concentration of antibody are most extreme the mouse is yielded and the spleen is taken. Then it is separated into individual spleenocyte by applying enzyme or instrumental process. Lymphocytes of the spleen is separated from rest of the organelles by Density gradient centrifugation . ...
https://modernloveok.com/hybridoma-technology-is-applied-to-induce-a-hybrid-cell-by-fusing-beta-lymphocytes-with-myeloma-cell/
IL-10 Antibody, anti-human | Hybridomas | MACS Antibodies | Products | Miltenyi Biotec | Canada
Interleukin 10 (IL‑10) is a cytokine predominantly secreted by CD4+ memory and effector T cells and antigen-presenting cells, for example, monocytes/macrophages and dendritic cells. IL-10 has important suppressive functions on immune responses and is believed to be involved in the maintenance of tolerance. IL-10 blocks activation of cytokine synthesis by Tʜ1 cells, activated monocytes, and NK cells. It can stimulate immunoglobulin production by B cells.The Anti-IL‑10 antibodies has been designed for intracellular staining of IL‑10-producing cells. Cells can be stimulated for IL‑10 production, for example, by polyclonal stimulation with mitogens. For induction of IL‑10 production by antigen-specific T cells, cells are re-stimulated with the respective antigen. IL‑10 can be accumulated in the cells by addition of secretion inhibitors like brefeldin A. After fixation and permeabilization of the cell sample, IL‑10-producing cells can be stained intracellularly with Anti-IL‑10 antibodies.
https://www.miltenyibiotec.com/CA-en/products/il-10-antibody-anti-human-jes3-9d7.html
Positive Tested Species Reactivity: Drosophila-melanogaster, Insects, Clonality: MonoclonalPositive Tested Species Reactivity: Drosophila-melanogaster, Insects, Clonality: Monoclonal
Hybridoma technology is used to fuse fusion a B cell and myeloma to form a hybridoma that produces identical monoclonal antibodies.
https://dshb.biology.uiowa.edu/monoclonal/custitem34/Drosophila-melanogaster,Insects/custitem36/Monoclonal
Positive Tested Species Reactivity: Wallaby, Clonality: MonoclonalPositive Tested Species Reactivity: Wallaby, Clonality: Monoclonal
Hybridoma technology is used to fuse fusion a B cell and myeloma to form a hybridoma that produces identical monoclonal antibodies.
https://dshb.biology.uiowa.edu/monoclonal/custitem34/Wallaby/custitem36/Monoclonal
Hybridoma technologyHybridoma technology
The hybridomas can be grown in culture, each culture starting with one viable hybridoma cell, producing cultures each of which ... Hybridomas at the US National Library of Medicine Medical Subject Headings (MeSH) "Hybridoma Technology". Understanding Cancer ... Once a hybridoma colony is established, it will continually grow in culture medium like RPMI-1640 (with antibiotics and fetal ... Hybridoma technology is a method for producing large numbers of identical antibodies (also called monoclonal antibodies). This ...
https://en.wikipedia.org/wiki/Hybridoma_technology
Rabbit hybridomaRabbit hybridoma
A rabbit hybridoma is a hybrid cell line formed by the fusion of an antibody producing rabbit B cell with a cancerous B-cell ( ... "Recombinant human interleukin-6 enhances the immunoglobulin secretion of a rabbit-rabbit hybridoma". Hybridoma. 20 (3): 189-98 ... The process of hybridoma formation in a rabbit first entails obtaining B-cells from a rabbit that has been immunized. There are ... Resulting antibodies from hybridomas are screened for an antigen which meets criteria of interest by diagnostic tests such as ...
https://en.wikipedia.org/wiki/Rabbit_hybridoma
Developmental Studies Hybridoma BankDevelopmental Studies Hybridoma Bank
7 hybridoma deposits) RIKEN Stephen T. Warren (1 hybridoma deposit) Emory University Zena Werb (1 hybridoma deposit) University ... Wieschaus deposited 7 hybridomas National Academy of Sciences Members who have deposited hybridomas Utpal Banerjee (1 hybridoma ... 1 hybridoma deposit) Harvard University Max Cooper (1 hybridoma deposit) Emory University Marilyn Gist Farquhar (1 hybridoma ... There are currently over 5000 hybridomas in the DSHB collection. The DSHB has obtained hybridomas from a variety of individuals ...
https://en.wikipedia.org/wiki/Developmental_Studies_Hybridoma_Bank
IGHDIGHD
Antibodies Hybridomas. 3 (2): 65-74. doi:10.3233/HAB-1992-3202. PMID 1633267. Roes J, Rajewsky K (1992). "Cell autonomous ...
https://en.wikipedia.org/wiki/IGHD
LSP1LSP1
Hybridoma. 17 (6): 497-507. doi:10.1089/hyb.1998.17.497. PMID 9890705. Pulford K, Jones M, Banham AH, et al. (1999). " ...
https://en.wikipedia.org/wiki/LSP1
Collagen hybridizing peptideCollagen hybridizing peptide
Hybridoma. 19 (5): 375-385. doi:10.1089/02724570050198893. ISSN 0272-457X. PMID 11128027. Li, Y.; Foss, C. A.; Summerfield, D. ...
https://en.wikipedia.org/wiki/Collagen_hybridizing_peptide
ASPHASPH
Hybridoma. 28 (4): 251-7. doi:10.1089/hyb.2009.0017. PMID 19663697. Palumbo KS, Wands JR, Safran H, King T, Carlson RI, de la ...
https://en.wikipedia.org/wiki/ASPH
AMY1AAMY1A
1986). "Pancreatic amylase expression in human pancreatic development". Hybridoma. 5 (2): 137-45. doi:10.1089/hyb.1986.5.137. ...
https://en.wikipedia.org/wiki/AMY1A
Heterophile antibodyHeterophile antibody
Hybridoma. 11 (1): 33-9. doi:10.1089/hyb.1992.11.33. PMID 1737638. Bolstad N, Warren DJ, Nustad K (October 2013). "Heterophilic ...
https://en.wikipedia.org/wiki/Heterophile_antibody
ICOSLGICOSLG
Hybridoma. 30 (4): 361-368. doi:10.1089/hyb.2011.0014. PMID 21851236. Khayyamian S, Hutloff A, Büchner K, Gräfe M, Henn V, ...
https://en.wikipedia.org/wiki/ICOSLG
Anti-actin antibodiesAnti-actin antibodies
Hybridoma. 17 (5): 431-5. doi:10.1089/hyb.1998.17.431. PMID 9873988. Enright, Helen (Oct 1995). "Paraneoplastic autoimmune ...
https://en.wikipedia.org/wiki/Anti-actin_antibodies
ZBTB33ZBTB33
Hybridoma. 20 (3): 159-66. doi:10.1089/027245701750293484. PMID 11461664. Yoon HG, Chan DW, Reynolds AB, Qin J, Wong J (Sep ...
https://en.wikipedia.org/wiki/ZBTB33
Camptodactyly-arthropathy-coxa vara-pericarditis syndromeCamptodactyly-arthropathy-coxa vara-pericarditis syndrome
Hybridoma. 20 (3): 149-57. doi:10.1089/027245701750293475. PMID 11461663. (Articles with short description, Short description ...
https://en.wikipedia.org/wiki/Camptodactyly-arthropathy-coxa_vara-pericarditis_syndrome
MonocyteMonocyte
Hybridoma. 7 (6): 521-527. doi:10.1089/hyb.1988.7.521. PMID 2466760. Passlick, Bernward; Flieger, Dimitri; Ziegler-Heitbrock, H ...
https://en.wikipedia.org/wiki/Monocyte
Hedgehog signaling pathwayHedgehog signaling pathway
Hybridoma. 26 (4): 231-40. doi:10.1089/hyb.2007.0507. PMID 17725385. Stecca B, Mas C, Ruiz i Altaba A (May 2005). "Interference ...
https://en.wikipedia.org/wiki/Hedgehog_signaling_pathway
ING1ING1
Hybridoma and Hybridomics. 21 (1): 1-10. doi:10.1089/15368590252917584. PMID 11991811. Bromidge T, Lynas C (Jul 2002). " ...
https://en.wikipedia.org/wiki/ING1
Cell cultureCell culture
The National Centre for Cell Science (NCCS), Pune, India; national repository for cell lines/hybridomas etc. Public Health ... to produce a hybridoma which has the antibody specificity of the primary lymphocyte and the immortality of the myeloma. ...
https://en.wikipedia.org/wiki/Cell_culture
Bispecific monoclonal antibodyBispecific monoclonal antibody
In 1975, the problem of producing pure antibodies was solved by the creation of hybridoma technology, and the new era of ... These BsAbs are often manufactured with the quadroma, or the hybrid hybridoma, method. However, the quadroma method relies on ... In 1983, Milstein and Cuello created hybrid-hybridoma (quadroma) technology. In 1988, the single-chain variable fragment (scFv ... S2CID 4161444.(subscription required) Milstein C, Cuello AC (October 1983). "Hybrid hybridomas and their use in ...
https://en.wikipedia.org/wiki/Bispecific_monoclonal_antibody
Juliet DanielJuliet Daniel
1999) Hybridoma 18: 343-349. 35. Daniel JM and Reynolds AB. The catenin p120ctn interacts with Kaiso, a novel BTB/POZ domain ... 2001) Hybridoma 20: 159-166. 33. Thoreson MA, Anastasiadis PZ, Daniel JM, Ireton RC, Wheelock MJ, Johnson KR, Hummingbird DK ...
https://en.wikipedia.org/wiki/Juliet_Daniel
Myc-tagMyc-tag
"Source for 9E10 anti-myc hybridoma". 2009-09-22. (Biochemistry, Peptide sequences). ... is available from the non-commercial Developmental Studies Hybridoma Bank. Protein tag Polyhistidine-tag SpyTag Flag-tag Evan ...
https://en.wikipedia.org/wiki/Myc-tag
HAT mediumHAT medium
This process is called hybridoma technology. Laboratory animals (e.g., mice) are first exposed to an antigen against which we ...
https://en.wikipedia.org/wiki/HAT_medium
Organization and expression of immunoglobulin genesOrganization and expression of immunoglobulin genes
"Organization and expression of immunoglobulin genes in fetal liver hybridomas". Proceedings of the National Academy of Sciences ...
https://en.wikipedia.org/wiki/Organization_and_expression_of_immunoglobulin_genes
Activation-induced cytidine deaminaseActivation-induced cytidine deaminase
"Activation-induced cytidine deaminase turns on somatic hypermutation in hybridomas". Nature. 415 (6873): 802-6. doi:10.1038/ ...
https://en.wikipedia.org/wiki/Activation-induced_cytidine_deaminase
Monoclonal antibodyMonoclonal antibody
The hybridomas can be grown indefinitely in a suitable cell culture medium. They can also be injected into mice (in the ... The medium must be enriched during in vitro selection to further favour hybridoma growth. This can be achieved by the use of a ... Rabbit B-cells can be used to form a rabbit hybridoma. Polyethylene glycol is used to fuse adjacent plasma membranes, but the ... Only fused hybrid cells referred to as hybridomas, are able to grow indefinitely in the medium because the spleen cell partner ...
https://en.wikipedia.org/wiki/Monoclonal_antibody
Dilution cloningDilution cloning
"Cloning by Limiting Dilution of Hybridoma". (All articles with dead external links, Articles with dead external links from July ...
https://en.wikipedia.org/wiki/Dilution_cloning
Leonard HerzenbergLeonard Herzenberg
During a sabbatical in the laboratory of Cesar Milstein between 1976 and 1977, Herzenberg coined the term hybridoma for hybrid ... "The hybridoma revolution: an offshoot of basic research". BioEssays. 21 (11): 966-973. doi:10.1002/(SICI)1521-1878(199911)21:11 ...
https://en.wikipedia.org/wiki/Leonard_Herzenberg
César MilsteinCésar Milstein
The term hybridoma was proposed by Len Herzenberg during a sabbatical in my laboratory in 1976/1977. At a high-table ... The term hybridoma was coined by Leonard Herzenberg during his sabbatical in Milstein's laboratory between 1976 and 1977. ... The development of the hybridoma technology coupled to advances in nucleic acid sequencing allowed Milstein to chart the ... Milstein, César (11 October 1999). "The hybridoma revolution: an offshoot of basic research". BioEssays. 21 (11): 966-973. doi: ...
https://en.wikipedia.org/wiki/César_Milstein
DonanemabDonanemab
Little M, Kipriyanov SM, Le Gall F, Moldenhauer G (August 2000). "Of mice and men: hybridoma and recombinant antibodies". ...
https://en.wikipedia.org/wiki/Donanemab
Cell fusionCell fusion
For production of monoclonal antibodies by producing hybridoma. For production of Induced stem cells. To assess protein ...
https://en.wikipedia.org/wiki/Cell_fusion
Douglas R. GreenDouglas R. Green
Shi, Y. F.; Sahai, B. M.; Green, D. R. (1989). "Cyclosporin a inhibits activation-induced cell death in T-cell hybridomas and ... "Activation-induced cell death in T cell hybridomas is due to apoptosis. Morphologic aspects and DNA fragmentation". Journal of ... "Role for c-myc in activation-induced apoptotic cell death in T cell hybridomas". Science. 257 (5067): 212-4. Bibcode:1992Sci... ...
https://en.wikipedia.org/wiki/Douglas_R._Green
Browsing by Subject HybridomasBrowsing by Subject "Hybridomas"
Hybridoma technology with special reference to parasitic diseases : a notebook of papers and techniques presented at two ... Properties of the monoclonal antibodies produced by hybridoma technology and their application to the study of diseases : ... 1-5 October l979 and Hybridoma Technology with Special Reference to Parasitic Diseases, held in Belo Horizonte, Brazil, 19-23 ...
https://extranet.who.int/iris/restricted/browse?authority=Hybridomas&type=mesh
Antibody Discovery via Hybridomas | Molecular DevicesAntibody Discovery via Hybridomas | Molecular Devices
Optimizing the antibody discovery workflow from fusion to clone selection can dramatically accelerate hybridoma screening and ... Hybridoma Workflow In this video, Justin Dranschak presents Molecular Devices solution for a hybridoma workflow. Watch this ... now called a hybridoma cell. Because every B-cell produces a unique antibody, single-cell cloning of hybridomas can be used to ... Hybridoma workflow. Step 1: Fusion The process of fusing B cells, expressing unique antibodies, with myeloma cells creating a ...
https://www.moleculardevices.com/applications/monoclonal-antibodies-mabs/hybridoma
Contact - Antibody Hybridoma Core - Mayo Clinic ResearchContact - Antibody Hybridoma Core - Mayo Clinic Research
Contact the Antibody Hybridoma Core at Mayo Clinics campus in Rochester, Minnesota, to request services or schedule a ... Antibody Hybridoma Core. *Mayo Clinic. Guggenheim Building, Third Floor, Room 303. 200 First St. SW. Rochester, MN 55905 ...
https://www.mayo.edu/research/core-resources/antibody-hybridoma-core/contact
Anti-α-Tubulin antibody, Mouse monoclonal clone DM1A, purified from hybridoma cell culture | Sigma-AldrichAnti-α-Tubulin antibody, Mouse monoclonal clone DM1A, purified from hybridoma cell culture | Sigma-Aldrich
... purified from hybridoma cell culture; Suitable for immunoprecipitation (IP); Anti-α-Tubulin antibody, Mouse monoclonal has been ... Anti-α-Tubulin antibody, Mouse monoclonal (mouse IgG1 isotype) is derived from the hybridoma DM1A produced by the fusion of ...
https://www.sigmaaldrich.com/ES/es/product/sigma/t6199
TCR-mediated adhesion of T cell hybridomas to planar bilayers containing purified MHC class II/peptide complexes and receptor...TCR-mediated adhesion of T cell hybridomas to planar bilayers containing purified MHC class II/peptide complexes and receptor...
TCR-mediated adhesion of T cell hybridomas to planar bilayers containing purified MHC class II/peptide complexes and receptor ... specific T cell hybridoma, 3A9, to artificial bilayers containing covalent MHC class II/peptide complexes or adhesion molecules ...
https://pubmed.ncbi.nlm.nih.gov/8757322/
A novel selection strategy for antibody producing hybridoma cells based on a new transgenic fusion cell line | Scientific...A novel selection strategy for antibody producing hybridoma cells based on a new transgenic fusion cell line | Scientific...
The stably transfected myeloma cell line was used for the generation of hybridoma cells and an antigen- and isotype-specific ... based on an artificial cell surface construct by which secreted antibodies were connected to the corresponding hybridoma cell ... Isotype- and antigen-specific sorting of HA-AP-EGF-R+ hybridoma cells. Ten days after fusion HA-AP-EGF-R+ hybridoma cells were ... Staining of HA-AP-EGF-R bearing hybridoma cells. HA-AP-EGF-R bearing hybridoma cells were stained for receptor expression using ...
https://www.nature.com/articles/s41598-020-58571-w?error=cookies_not_supported
Antibodies & Hybridomas - page 1 of 12Antibodies & Hybridomas - page 1 of 12
To get the most hits and fastest results, use as few words as possible in your search string ...
https://www.linscottsdirectory.com/antibodies?q=pbr
Why Use Semi-Solid Medium for Cloning Hybridomas and CHO Cells | STEMCELL TechnologiesWhy Use Semi-Solid Medium for Cloning Hybridomas and CHO Cells | STEMCELL Technologies
Cloning hybridomas and CHO cells can be done faster and more efficiently using semi-solid cloning, compared to traditional ... Why Use Semi-Solid Medium for Cloning Hybridomas and CHO Cells Cloning hybridomas and CHO cells can be done faster and more ... Cloning hybridomas and CHO cells can be done faster and more efficiently using semi-solid cloning. During semi-solid cloning, ... Semi-solid methylcellulose-based medium for hybridoma selection and cloning, without HAT (serum-containing) ...
https://www.stemcell.com/semi-solid-cloning-with-clonacell.html
Hybridoma (Larchmt) | Scholars@DukeHybridoma (Larchmt) | [email protected]
Hybridoma (Larchmt) * Publication Venue For. * In vivo electroporation and non-protein based screening assays to identify ...
https://scholars.duke.edu/display/jou1557-8348
CD3 Antibody, anti-human | Hybridomas | MACS Antibodies | Products | Miltenyi Biotec | España
The CD3 antibody recognizes the human CD3 antigen which is present on mature human T cells, thymocytes, and NKT cells. The epitope recognized by the antibody is located on the ε-chain of the CD3 complex. Clone OKT3 has been shown to activate T cell function.Activation and proliferation of T cells can be induced by interaction of the T cell receptor with peptide-MHC complexes expressed on the surface of antigen-presenting cells (APCs). The T cell receives a signal transduced through the CD3 complex. Cytokines or other costimulatory signals are required in addition. Activated T cells can be used for any downstream processes, such as cytokine analysis or immunoprecipitation. Cells can also be transfected with high efficiency. | España
https://www.miltenyibiotec.com/ES-en/products/cd3-antibody-anti-human-okt3.html
Genetic control of immunity to Heligmosomoides polygyrus: Presentation of promiscuous antigens to parasite-specific T cell...
... using a number of T cell hybridomas produced in A and E positive and negative mice. By using fixed and irradiated antigen ... using a number of T cell hybridomas produced in A and E positive and negative mice. By using fixed and irradiated antigen ... using a number of T cell hybridomas produced in A and E positive and negative mice. By using fixed and irradiated antigen ... using a number of T cell hybridomas produced in A and E positive and negative mice. By using fixed and irradiated antigen ...
https://mayoclinic.pure.elsevier.com/en/publications/genetic-control-of-immunity-to-heligmosomoides-polygyrus-presenta
Hybridoma
... -- Vol.1, no.1 (1982) - Vol.20, no.4 (2001) -.-- New York ISSN 0272-457X. ...
http://portalrev.enfermagem.bvs.br/index.php?mfn=13379&about=access%E2%8C%A9=pt
Growth of hybridoma cells under different agitation conditions - Fingerprint - Experts@MinnesotaGrowth of hybridoma cells under different agitation conditions - Fingerprint - [email protected]
Dive into the research topics of Growth of hybridoma cells under different agitation conditions. Together they form a unique ...
https://experts.umn.edu/en/publications/growth-of-hybridoma-cells-under-different-agitation-conditions/fingerprints/
Anti-Stagonospora nodorum hybridoma - Oxford University InnovationAnti-Stagonospora nodorum hybridoma - Oxford University Innovation
Stagonospora nodorum is an economically important foliar pathogen of cereals. The information presented here on these pages has been provided by the scientists and academics who have created the reagents. As far as Oxford University Innovation is able to establish this information is accurate but no representation is given as to its accuracy or completeness. It is the responsibility of the customer to determine the suitability for any purpose of the reagents and Oxford University Innovation shall not be liable for any claims, losses, liabilities, expenses or damages arising from any use of the materials. Nothing on these pages will form part of any contract or licence nor be regarded as a warranty or representation in relation to the products. Any supply would be subject to the terms and conditions of a separate agreement. Oxford University Innovation makes no representation that any item in this catalogue is not subject to third party intellectual property rights.. ...
https://innovation.ox.ac.uk/materials/anti-stagonospora-nodorum-economically-important-foliar-pathogen-cereals-2/
Glycosylation of antibody in a lectin-resistant human hybridoma is insensitive to glucose<...
Tachibana H, Ushio Y, Murakami H. Glycosylation of antibody in a lectin-resistant human hybridoma is insensitive to glucose. In ... Tachibana, H, Ushio, Y & Murakami, H 1995, Glycosylation of antibody in a lectin-resistant human hybridoma is insensitive to ... Glycosylation of antibody in a lectin-resistant human hybridoma is insensitive to glucose. / Tachibana, Hirofumi; Ushio, ... Tachibana, H., Ushio, Y., & Murakami, H. (1995). Glycosylation of antibody in a lectin-resistant human hybridoma is insensitive ...
https://kyushu-u.pure.elsevier.com/en/publications/glycosylation-of-antibody-in-a-lectin-resistant-human-hybridoma-i
Human antibodies and hybridomas
... / Butterworth Heinemann Publishers.-- Vol.1, no.1 (1990) - Vol.7, no.4 (1996) -.-- Stoneham ...
http://portalrev.enfermagem.bvs.br/index.php?issn=0956-960X&lang=pt
Results of search for 'su:{Hybridomas}' › WHO HQ Library catalog
Hybridoma technology with special reference to parasitic diseases. by UNDP/World Bank/WHO Special Programme for Research and ... Hybridoma technology with special reference to parasitic diseases : a notebook of papers and techniques presented at two ... Properties of the monoclonal antibodies produced by hybridoma technology and their application to the study of diseases : ... Properties of the monoclonal antibodies produced by hybridoma technology and their application to the study of diseases : ...
https://kohahq.searo.who.int/cgi-bin/koha/opac-search.pl?q=su:%7BHybridomas%7D
Details for: Monoclonal antibodies and T-cell hybridomas : › WHO HQ Library catalog
Monoclonal antibodies and T-cell hybridomas : perspectives and technical advances / editors, Günter J. Hämmerling, Ulrich ... Hybridomas -- immunology , T-LymphocytesNLM classification: QW 575 ...
https://kohahq.searo.who.int/cgi-bin/koha/opac-search.pl?q=ccl=Provider%3AElsevier%20%20and%20se%3AResearch%20monographs%20in%20immunology&sort_by=relevance_dsc&count=20&limit=au:H%C3%A4mmerling,%20G%C3%BCnter%20J.
Catalog #24102 - Cloning and sequencing of rat hybridoma IgG variable regions - Curia GlobalCatalog #24102 - Cloning and sequencing of rat hybridoma IgG variable regions - Curia Global
Cloning and sequencing of rat hybridoma IgG variable regions ... Cloning and sequencing of rat hybridoma IgG variable regions. ... 1. Extract and purify total RNA from hybridoma cells or clonal B cell. ... Catalog #24101 - Cloning and sequencing of mouse hybridoma IgG variable regions ... Catalog #24103 - Cloning and sequencing of rabbit hybridoma IgG variable regions ...
https://curiaglobal.com/biologics/antibody-production/antibody-variable-region-sequencing-and-cloning/catalog-24102-cloning-and-sequencing-of-rat-hybridoma-igg-variable-regions/
Hybridoma Sequencing Pitfalls - Abterra BiosciencesHybridoma Sequencing Pitfalls - Abterra Biosciences
Learn about common mistakes made during hybridoma sequencing projects. ... Impurity of cells/hybridomas: Diluting and culturing hybridomas as monoclonal is not always accurate, so a sample can ... Abterra Bio uses NGS for hybridoma sequencing and our software can assess clonality of your hybridomas. We will give you access ... Not all hybridomas produce monoclonal antibodies. In addition, a sample could have contamination of other organisms like ...
https://abterrabio.com/sequencing-pitfalls-resources/
Core - Hybridoma/Immunohistochemistry - Carol WikstrandCore - Hybridoma/Immunohistochemistry - Carol Wikstrand
Core - Hybridoma/Immunohistochemistry Wikstrand, Carol J. Duke University, Durham, NC, United States ...
https://grantome.com/grant/NIH/P30-CA014236-27-9037
UltraDOMA-PF Hybridoma Medium | RUWAG SWITZERLANDUltraDOMA-PF Hybridoma Medium | RUWAG SWITZERLAND
Monoclonal Antibody Development | Hybridoma Development | Antibody GenerationMonoclonal Antibody Development | Hybridoma Development | Antibody Generation
Hybridoma & Cell Lines Rescue Services , Immune Response Assays , Monoclonal Antibody & Hybridoma Development Services , ... Monoclonal Antibody and Hybridoma Development Services. One of our core competency and expertise is in development of new ... hybridoma monoclonal antibody. Our key personals have over 25 years of expertise in this area, making many monoclonal ...
https://antibodyresearch.com/monoclonal-antibody-and-hybridoma-development-services/
China good quality Custom Monoclonal Antibody on salesChina good quality Custom Monoclonal Antibody on sales
Methadone Hybridoma Metabolization Monoclonal Anti body from China, Chinas leading mouse monoclonal antibodies product, with ... Custom Monoclonal Antibody Hybridoma Monoclonal Antibody Mouse Monoclonal Antibodies Drug Abuse Test Kit Rapid Test Kit ... Mouse anti-Methadone Hybridoma Monoclone Antibody Drug of Abuse For In Vitro Research ... Mouse anti-Methadone Hybridoma Monoclone Antibody is the high performance product to meet ...
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Science Clips - Monday, July 19, 2010Science Clips - Monday, July 19, 2010
Hybridoma. 2010 Jun;29(3):271-271.. *Comparative analysis of direct fluorescence, zenon labeling, and quantum dot nanocrystal ...
https://www.cdc.gov/library/sciclips/issues/v2issue29.html
Semi-solid cloning of mouse hybridomas to generate mouse monoclonal antibodiesSemi-solid cloning of mouse hybridomas to generate mouse monoclonal antibodies
This video outlines the steps in semi-solid cloning of mouse hybridomas to generate mouse monoclonal antibodies using ClonaCell ... This video outlines the steps in semi-solid cloning of mouse hybridomas to generate mouse monoclonal antibodies using ClonaCell ... Semi-solid cloning of mouse hybridomas to generate mouse monoclonal antibodies. March 17, 2014 ...
https://cellculturedish.com/semi-solid-cloning-mouse-hybridomas-generate-mouse-monoclonal-antibodies/
In vitro antibody synthesis in 20 μl hanging drops: Initiation of secondary responses and a simple method of cloning hybridomas...In vitro antibody synthesis in 20 μl hanging drops: Initiation of secondary responses and a simple method of cloning hybridomas...
In vitro antibody synthesis in 20 μl hanging drops: Initiation of secondary responses and a simple method of cloning hybridomas ... In vitro antibody synthesis in 20 μl hanging drops: Initiation of secondary responses and a simple method of cloning hybridomas ... The hanging drop method also provides a reliable yet simple procedure of cloning hybridoma cells while permitting direct ... The hanging drop method also provides a reliable yet simple procedure of cloning hybridoma cells while permitting direct ...
https://ohsu.pure.elsevier.com/en/publications/in-vitro-antibody-synthesis-in-20-%CE%BCl-hanging-drops-initiation-of--2
Hybridoma Sequencing Service - KMD Bioscience-Professional CRO Service Supplier
The acquisition of the monoclonal antibody gene sequence of hybridoma cells is the basis for recombinant antibodies and the ... Hybridoma Sequencing Service. The acquisition of the monoclonal antibody gene sequence of hybridoma cells is the basis for ... Customers can provide antigen and hybridoma cell lines directly. --Customers can provide antigens, hybridoma cell culture ... In the process of preparing recombinant antibodies, after customer supplies the hybridoma cells, there are two heavy chain and ...
http://kmdbioscience.com/sv-Hybridoma-Sequencing-Service
Difference between revisions of "Hybridoma cells clones" - Tanaka Wiki
for cloning: use Hybridoma cloning factor (HCF) (Hybrid - max Hybridoma media supplement #ECO1021N (50 ml)) use @ 10 % ... Thawing of Hybridoma cells 1) thaw a vial of cells quickly @ 37 degree 2) transfer into 10 ml fresh High serum media 3) ... Passaging of Hybridoma cells Materials: - HS- media - 15 ml falcon tube - 25 cm2 corning cell culture flasks (Pax 7 cells need ... Thaw the 1st AB (f.ex.: mouse anti- Pax7 - Hybridoma supernatants) @Rt or ...
https://wiki.tanakalab.org/index.php?title=Hybridoma_cells_clones&diff=prev&oldid=806
2019 AACC: Exhibitors2019 AACC: Exhibitors
Will your company display a new product or promote a new indication/enhanced feature(s) for an existing product in your booth ...
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AntibodiesCellsSpecificityPhage displayAntigenClonesClonalityMiceAssayCloningProliferationCulturesTechnologyCovalentReferencesMediumHumanPageCoreTechniqueAntibodiesAntibodySupernatantDEVELOPMENTAL STUDHelper T cell hybridomConjugate1984MabsTechnologySuppressorSerum-FreeCharacterizationCloneViabilityGenerationVitroLymphocyteProduction1989ProteinsSuitableFacilityFactorSegmentGene
Antibodies25
  • Hybridoma technology is a method for mass-producing antibodies in a hybrid cell line generated from the fusion of antibody-producing B-cells with an immortalized myeloma cell line, now called a hybridoma cell. (moleculardevices.com)
  • Because every B-cell produces a unique antibody, single-cell cloning of hybridomas can be used to generate a diverse library of unique monoclonal antibodies at a large scale, which are very frequently used in the prevention, diagnosis, and treatment of disease. (moleculardevices.com)
  • The process of fusing B cells, expressing unique antibodies, with myeloma cells creating a hybrid cell line is called a hybridoma. (moleculardevices.com)
  • The identification of clonally-derived hybridoma cell lines which are producing high amounts of monoclonal antibodies. (moleculardevices.com)
  • To address these issues we developed a novel selective technology based on an artificial cell surface construct by which secreted antibodies were connected to the corresponding hybridoma cell when they possess the desired antigen-specificity. (nature.com)
  • Especially the hybridoma technique which results in full-length monoclonal antibodies can be cumbersome, labour-intensive and time-consuming (Fig. 1A ). (nature.com)
  • To facilitate the isolation of specific antibody-producing hybridomas, a method has to be established which temporarily restricts the cells from releasing the antibody into the culture medium and thus retaining the genotype (the antibody-coding genes) and the phenotype (the produced antibodies) in one entity. (nature.com)
  • Human antibodies and hybridomas / Butterworth Heinemann Publishers. (bvs.br)
  • Monoclonal antibodies and T-cell hybridomas : perspectives and technical advances / editors, Günter J. Hämmerling, Ulrich Hämmerling and John F. Kearney. (who.int)
  • NGS provides greater sequencing depth and would be able to capture polyclonal antibodies from a hybridoma sample. (abterrabio.com)
  • Not all hybridomas produce monoclonal antibodies. (abterrabio.com)
  • 1999. 1, Generation of Hybridomas: Permanent Cell Lines Secreting Monoclonal Antibodies. (abterrabio.com)
  • This video outlines the steps in semi-solid cloning of mouse hybridomas to generate mouse monoclonal antibodies using ClonaCell™-HY. (cellculturedish.com)
  • The acquisition of the monoclonal antibody gene sequence of hybridoma cells is the basis for recombinant antibodies and the development of antibody drugs. (kmdbioscience.com)
  • In the process of preparing recombinant antibodies, after customer supplies the hybridoma cells, there are two heavy chain and two light chain problems, which leads to the diversity of subsequent light and heavy chain pairing combinations, making the preparation of recombinant antibodies time-consuming and labor-intensive. (kmdbioscience.com)
  • King, G 2014, ' Klotho MAb: Hybridoma Lines KL-115 and KL-234 ', Monoclonal Antibodies in Immunodiagnosis and Immunotherapy , vol. 33, no. 6, pp. 449. (elsevier.com)
  • The Antibody and Bioconjugates Discovery group at NIBR/NBC-San Diego is seeking an antibody expert to lead a powerful hybridoma platform for discovery of monoclonal antibodies. (gijobs.com)
  • Our RabMAb ® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. (abcam.com)
  • Thesis: Investigation of the hybridomas stabilities and neutralization capabilities of monoclonal antibodies developed against the glycoproteins of Crimean-Congo Hemorrhagic Fever Virus (CCHFV). (uni-muenster.de)
  • VICTORIA, July 13, 2020 / - IMMUNOPRECISE ANTIBODIES LTD. (the "Company" or "IPA") (TSX VENTURE: IPA) (OTCQB: IPATF) (FSE:TQB2), a leader in full-service, therapeutic antibody discovery and development, today announced the identification of additional, human lead candidate antibodies generated against SARS-CoV-2, also demonstrating potent in vitro neutralizing activity, discovered using the Company's B cell Select™ and Single Step Cloning Hybridoma Platforms. (ipatherapeutics.com)
  • More particularly it concerns murine monoclonal anti-human breast cancer antibodies, hybridomas that produce those antibodies, immunochemicals made from those antibodies, and diagnostic and therapeutic methods that use those immunochemicals. (justia.com)
  • The murine x murine hybridomas that produce the above described antibodies are progeny of those hybridomas are other aspects of the invention. (justia.com)
  • The affinity and kinetics of monoclonal antibodies produced from a hybridoma cell line cultured in two different types of medium were measured. (biopharminternational.com)
  • In this study, the authors evaluate the Octet QKe platform to establish an analytical method to assess media condition effect on the quality and activity of cultured antibodies by measuring the affinity and kinetics of monoclonal antibodies (mAbs) produced from a hybridoma cell line cultured in two different types of medium. (biopharminternational.com)
  • These homophilic antibodies can be immortalized as monoclonal antibodies using the hybridoma or phage display technique. (medscape.com)
Cells14
  • Anti-α-Tubulin antibody, Mouse monoclonal (mouse IgG1 isotype) is derived from the hybridoma DM1A produced by the fusion of mouse myeloma cells (NS1) and splenocytes from BALB/c mice immunized with purified chick brain tubulin. (sigmaaldrich.com)
  • The stably transfected myeloma cell line was used for the generation of hybridoma cells and an antigen- and isotype-specific screening method was established. (nature.com)
  • The picture (reprinted by permission from Springer Nature 10 ) shows the process of monoclonal antibody generation via conventional hybridoma technology ( A ) and via the new selection approach using transgenic fusion cell lines ( B ). The fusion with transgenic myeloma cells allows a fast and efficient hybridoma screening in an isotype- or antigen-specific manner and allows an early screening for possible cross-reactivities. (nature.com)
  • Cloning hybridomas and CHO cells can be done faster and more efficiently using semi-solid cloning. (stemcell.com)
  • 1. Extract and purify total RNA from hybridoma cells or clonal B cell. (curiaglobal.com)
  • The hanging drop method also provides a reliable yet simple procedure of cloning hybridoma cells while permitting direct visualization of the number of cells in each drop. (elsevier.com)
  • Mouse hybridoma cells producing monoclonal antibody against the 1465 antigenic particles of O type apthhoviruses were proliferated on the non-woven polyester fabric (NWPF) discs in stationary and stirred conditions. (hacettepe.edu.tr)
  • In this study, we established stable transfectants expressing two types of activin receptors, ActRI and ActRIB, to clarify the role of these receptors in activin signalling for growth inhibition in HS-72 mouse B-cell hybridoma cells. (elsevier.com)
  • These fused cells, called hybridomas, could be grown in culture. (si.edu)
  • Hybridoma cells: Yes. (cdc.gov)
  • A. W. Hohmann, L. Spatz, M. Irigoyen and A. Manheimer-Lory -- Introduction -- Immortalized B cells to probe the human antibody repertoire -- Infectious diseases -- Human immunodeficiency virus -- Erythrocyte antigens -- Human histocompatibility antigens -- Tumor antigens -- Autoantibodies -- Immortalization of B cells with EBV -- EBV transformation of B cells: methodology -- Making human hybridomas -- Hybridoma methodology -- References -- 4. (routledge.com)
  • Immune splenocytes were fused with mouse myeloma cells and hybridomas were selected based on some specificity of the culture media for breast or breast cancer antigens. (justia.com)
  • specimins, hybridoma cells, display cases. (cooperhewitt.org)
  • Hybridoma clones have been derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice immunized with recombinant SARS-CoV-2 Nucleoprotein (C518, C524, C527). (hytest.fi)
Specificity1
  • Specificity: Hybridoma tissue culture supernatants (CSN) were screened using an indirect ELISA as previously described. (cdc.gov)
Phage display1
  • Superseding hybridoma technology with phage display libraries. (routledge.com)
Antigen1
  • The critical issue in the development of antigen-specific hybridomas is the lack of any direct connection between the hybridoma cell and the released antibody. (nature.com)
Clones2
  • Our proprietary Mouse Quick-Hybridoma™ Platform allows generation of 50+ hybridoma clones per hybridoma cell fusion step. (advbiomart.net)
  • This platform is developed based on our new mouse adjuvant, FastAb™ Mouse Adjuvant , which enables generation of a large number of hybridoma clones using less immunogens. (advbiomart.net)
Clonality1
  • Abterra Bio uses NGS for hybridoma sequencing and our software can assess clonality of your hybridomas. (abterrabio.com)
Mice2
  • The role of MHC class II in the presentation of Heligmosomoides polygyrus antigens has been investigated, using a number of T cell hybridomas produced in A and E positive and negative mice. (elsevier.com)
  • A final boost of 50 mu g was given 3 weeks after the sixth immunization, and mice were sacrificed 3 days later for hybridoma production. (cdc.gov)
Assay1
  • All of these delta hIL-6 proteins were inactive in the IL-6 hybridoma growth factor (HGF) assay and unable to inhibit the HGF activity of the recombinant human wild-type IL-6 (wt hIL-6). (uniparthenope.it)
Cloning1
  • Bell, EB, Brown, M & Rittenberg, MB 1983, ' In vitro antibody synthesis in 20 μl hanging drops: Initiation of secondary responses and a simple method of cloning hybridomas ', Journal of Immunological Methods , vol. 62, no. 2, pp. 137-145. (elsevier.com)
Proliferation1
  • Complex subunit associated factors are involved in hybridoma growth, Eosinohils, eritroid proliferation and derived from promotor binding stimulating subunits on the DNA binding complex. (elisachina.com)
Cultures1
  • The medium elevates glucose and glutamine levels to deliver nutrients to the tissue-dense hollow-fiber hybridoma cultures. (the-scientist.com)
Technology2
  • Schematic overview about conventional hybridoma technology compared side by side to the new selection approach. (nature.com)
  • Hybridoma technology with special reference to parasitic diseases. (who.int)
Covalent1
  • To better understand the physical basis of the recognition stage of Ag presentation, we examined adhesion of the lysozyme- specific T cell hybridoma, 3A9, to artificial bilayers containing covalent MHC class II/peptide complexes or adhesion molecules. (nih.gov)
References1
  • In this video , Justin Dranschak, manager for BioPharma platforms, presents our solution for a hybridoma workflow and references the systems to aid in your research. (moleculardevices.com)
Medium1
  • Unisyn Technologies, based in San Diego, has introduced Hybrid Grow specialized basal medium for growing hybridomas in the company's Cell-Pharm hollow-fiber bioreactors. (the-scientist.com)
Human2
  • Tachibana, H , Ushio, Y & Murakami, H 1995, ' Glycosylation of antibody in a lectin-resistant human hybridoma is insensitive to glucose ', In Vitro Cellular & Developmental Biology - Animal: Journal of the Society for In Vitro Biology , vol. 31, no. 4, pp. 261-262. (elsevier.com)
  • Description: Description of target: The human interferon-beta 2 gene (IFNB2) product is identical to that for the B-cell stimulation factor-2 (BSF-2), the hybridoma growth factor (HGF) ("interleukin-6"), and the hepatocyte stimulating factor (HSF). (ayurvedandindia.com)
Page1
  • La liste des publications du groupe antérieure à 2014 est visible en bas de page (données saisies de façon manuelle). (ibs.fr)
Core1
  • One of our core competency and expertise is in development of new hybridoma monoclonal antibody. (antibodyresearch.com)
Technique1
  • To confer this basic principle to the hybridoma technique would require to capture the synthesized antibody on the surface of the synthesizing hybridoma cell (Fig. 1B ). (nature.com)
Antibodies22
  • The hybridoma collection of IZSLER Biobank contains an initial selection of those hybridoma that produce monoclonal antibodies regularly used in in-house diagnostic assays. (ibvr.org)
  • The hybridoma currently included in the Biobank are a sample of the many hybridoma generated during 30 years of research and development activities conducted at IZSLER to produce monoclonal antibodies against a wide spectrum of viruses, bacteria and proteins, mainly of veterinary interest, that were shown to be strategic tools for both research and diagnostic purposes. (ibvr.org)
  • The monoclonal antibodies expressed by each hybridoma are controlled through a series of immunological assays aimed at identifying and characterising their reactivity profile. (ibvr.org)
  • Moreover, the hybrid cell, called a hybridoma, pro-duces and secretes antibodies characteristic of the clone from which the normal lymphocyte is taken. (biologydiscussion.com)
  • Dr. Jean-Christophe Bourdon, Head of the p53 isoform laboratory at the University of Dundee, and his team have been using a CellMaker Plus 8L system to effectively produce a range of p53 antibodies from murine hybridoma cells. (cellexus.com)
  • B cell hybridomas expressing class I and II MHC molecules and producing antibodies directed against hemagglutinin protein of Rinderpest virus and human Mucin-1 have been used as surrogate B cells to study T cell responses against the antigens. (iisc.ac.in)
  • The hybridoma technology allows the production of large quantities of specific antibodies of a single isotype. (elsevier.com)
  • The critical chapters on generating monoclonal antibodies and growing hybridomas, which demystified hybridoma generation, have been greatly expanded and updated to make these procedures easy to follow and adaptable to current research needs. (cshlpress.com)
  • As Dr. Greenfield notes in his preface to this second edition: "The Antibodies manual provided our laboratory with guidance in the form of protocols and recommendations for setting up a hybridoma facility. (cshlpress.com)
  • Monoclonal antibodies can be produced using hybridomas. (labmanager.com)
  • We can isotype and sequence mouse or rat antibodies from hybridoma cell lines generated at the facility as well as cell lines from outside sources. (monash.edu)
  • Human Antibodies is an international journal designed to bring together all aspects of human hybridomas and antibody technology, along with factors that modulate host antibody repertoire and effectiveness, such as vaccines, infectious agents, and microbiome. (iospress.com)
  • There she learned the techniques of working with live cells, cell biology, hybridoma technology, and monoclonal antibodies. (nih.gov)
  • We report here the generation of hybridomas secreting monoclonal antibodies to rat lung fibroblast-pneumonocyte factor. (elsevier.com)
  • Recombinant antibodies have many advantages when compared to the typical hybridoma technology for producing monoclonal antibodies. (nih.gov)
  • The resultant hybrid cell or hybridoma has the capacity to produce antibodies of predetermined specificity and to grow "immortally" in culture. (irg-wp.com)
  • In contrast, hybridoma-based systems for producing monoclonal antibodies are subject to genetic drift and instability, increasing the potential for lot-to-lot variability or loss of antibody expression. (cellsignal.com)
  • Where polyclonal antibodies are purified directly from the serum of the immunized host, and monoclonals are purified from either hybridoma-derived tissue culture supernatant or ascites, recombinant antibodies are instead purified from the tissue culture supernatants of transfected host cell lines. (cellsignal.com)
  • A hybridoma cell can secrete and reproduce antibodies while proliferating indefinitely. (creativitea.org)
  • Monoclonal antibodies were produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, E. coli-derived, recombinant human IFNg. (sbhsciences.com)
  • Antibodies may also be defined through a deposited hybridoma cell producing the antibodies. (bdl-ip.com)
  • In Oct 2018, Immunoprecise Antibodies Ltd., one of the major player in life science market signed an agreement with 15 pharmaceutical companies to develop novel antibodies with the help of company's hybridoma technology. (fortunebusinessinsights.com)
Antibody14
  • Antigen-specific and antibody-mediated growth inhibition of suppressor T cell hybridomas. (harvard.edu)
  • Hybridoma are submitted to a double series of cloning procedure to assure the stability of the hybridoma and the clonality of the antibody produced. (ibvr.org)
  • To determine the sequence of the MAb, the cDNA of your antibody can be isolated from the hybridoma cell line and its subcloning and expression in mammalian cells. (exonbio.com)
  • Mouse anti- beta HCG Mab Hybridoma Monoclone Antibody is the high performance product to meet your different biological research and manufactory requirements. (custom-monoclonalantibody.com)
  • A series of human hybridomas were derived by the fusion of the immature T cell line , 1M , with lymphocytes from the peripheral blood of a colon carcinoma patient in long term remission who was shown to produce anti-tumor antibody . (musc.edu)
  • These hybridomas express the T cell surface markers CD2 , CD3 , CD4 , and CD8 and secrete human IgG2/kappa antibody . (musc.edu)
  • ClonaCell™-HY AOF Expansion Medium promotes robust expansion of hybridomas, adaptation from serum-containing to serum-free media, and stabilizing the viability and antibody productivity of hybridoma cell lines. (stemcell.com)
  • The absence of serum in this medium facilitates detection and purification of the desired hybridoma-derived antibody without interference from serum-derived immunoglobulins. (stemcell.com)
  • At first, VH and VL gene sequences were cloned from hybridoma cell lines secreting monoclonal antibody against LMG, and then thoroughly by database-assisted sequence analysis. (nih.gov)
  • The antibody sequencing protocol involves isolating the mRNA from hybridoma cells followed by cDNA synthesis and PCR amplification of heavy- and light-chain variable region genes. (monash.edu)
  • High-throughput screening for the identification of antibody hits in culture supernatants of hybridoma clones is usually performed in microtiter plates (MTPs) with 96 or more wells. (eppendorf.com)
  • Crystallization chaperones generated using recombinant technologies have emerged as superior alternatives that increase the throughput and eliminate inherent limitations associated with antibody production by animal immunization and the hybridoma technology. (nih.gov)
  • Diatec produces our very own fully human IgE antibody from a monoclonal hybridoma cell line. (diatec.com)
  • This antibody was produced from a hybridoma (mouse myeloma fused with spleen cells from a rat) immunized with mouse recombinant protein of CCL-25. (reliatech.de)
Supernatant2
  • We injected the hybridoma cells into the peritoneal cavity of pristane-primed F1 (AKR/J X BALB/c) mice, and a large amount of pure MNSF was obtained from the ascites, the characteristics of which were similar to those in the culture supernatant. (jimmunol.org)
  • Purified from hybridoma tissue culture supernatant. (enzolifesciences.com)
DEVELOPMENTAL STUD1
  • The reagents produced by this group are distributed at a minimal cost by the Developmental Studies Hybridoma Bank , ensuring broad availability and wide accessibility. (nih.gov)
Helper T cell hybridom1
  • cDNA libraries were constructed from one suppressor and two helper T cell hybridomas. (rupress.org)
Conjugate1
  • Hybridomas were screened in a solid-phase indirect enzyme-linked immunosorbent assay (ELISA) against a 2,4 TDI-human serum albumin (2,4 TDI-HSA) protein conjugate. (cdc.gov)
19841
Mabs2
  • Three hybridomas producing 2,4 TDI-HSA reactive IgM MAbs were obtained. (cdc.gov)
  • Moreover, hybridomas production or generation of mAbs usually takes around ten months. (creativitea.org)
Technology3
  • in Hybridoma Technology with Special Reference to Parasite Diseases, UNDP/World Bank, Geneva 1979, p. 65. (crossref.org)
  • Hybridoma technology was the first mAb production method that allowed for in vivo scientific research and is still the primary and preferred platform for mAb derivatisation (3). (cellexus.com)
  • I am a Fungal Immunologist with a specialist interest in hybridoma technology. (exeter.ac.uk)
Suppressor1
  • Isolation and characterization of a monoclonal nonspecific suppressor factor (MNSF) produced by a T cell hybridoma. (jimmunol.org)
Serum-Free2
  • ClonaCell™-HY AOF Expansion Medium is an animal origin-free (AOF) and serum-free liquid medium optimized for hybridoma expansion after hypoxanthine, aminopterin, thymidine (HAT) selection. (stemcell.com)
  • ab110304 was produced in vitro using hybridomas grown in serum-free medium. (abcam.com)
Characterization2
  • Iglesias-Ussel, MD, Zavadil, J & Scharff, MD 2009, ' Molecular characterization of hybridoma subclones spontaneously switching at high frequencies in vitro ', Journal of Immunological Methods , vol. 350, no. 1-2, pp. 71-78. (elsevier.com)
  • Five of these hybridomas selected for further characterization are the subject of this dissertation . (musc.edu)
Clone2
  • With this method, a stable E17 hybridoma clone was selected, and its product in culture medium was isolated and characterized. (jimmunol.org)
  • It is often observed that more than one clone is found in a monoclonal hybridoma cell line. (exonbio.com)
Viability1
  • In most cases, cryopreserved hybridomas can be thawed directly into ClonaCell™-HY AOF Expansion Medium while maintaining a high level of viability. (stemcell.com)
Generation2
  • Generation of functional single-chain fragment variable from hybridoma and development of chemiluminescence enzyme immunoassay for determination of total malachite green in tilapia fish. (nih.gov)
  • Generation of hybridomas secret. (uncg.edu)
Vitro2
  • We have shown previously that forced expression of activation induced cytidine deaminase (AID) in hybridomas increased their very low frequency of class switch recombination (CSR) in vitro only ∼ 7-13 fold. (elsevier.com)
  • Since we had previously identified rare hybridoma subclones that spontaneously switched at more than 100 times higher frequencies, we have now examined those higher switching variants to search for ways to further increase the frequency of isotype switching in vitro. (elsevier.com)
Lymphocyte1
  • The hybridomas will proliferate because the lymphocyte nuclei con-tribute functional HGPRT genes to the heterokaryon. (biologydiscussion.com)
Production2
  • In this report we describe a functional role for Shc in two events that occur during T cell activation, AICD and IL-2 production, in a T cell hybridoma line that has been previously shown to undergo apoptosis and produce IL-2 upon cross-linking of the TCR/CD3 complex. (jimmunol.org)
  • Splenocytes were isolated 24 h after the last exposure for hybridoma production. (cdc.gov)
19891
Proteins1
  • Thus, the MNSF obtained from the E17 hybridoma consists of functionally identical but physicochemically different discrete proteins. (jimmunol.org)
Suitable1
  • The particular hybridomas producing the desired anti-body can be identified using a suitable assay proce-dure, and these can be selected and separately cloned. (biologydiscussion.com)
Facility1
  • Paul Kapke, an associate scientist in the Office of Biotechnology and manager of the Hybridoma Facility, is retiring May 31. (iastate.edu)
Factor1
  • A cytokine that stimulates the growth and differentiation of B-LYMPHOCYTES and is also a growth factor for HYBRIDOMAS and plasmacytomas. (nih.gov)
Segment1
  • The hybridomas segment is also expected to register considerable revenue growth in the years to come. (pharmiweb.com)
Gene3
  • Three T cell hybridomas do not contain detectable heavy chain variable gene transcripts. (rupress.org)
  • AID was not responsible for the ∼ 100 fold increase in CSR, so we used whole-genome gene expression profiling to provide a platform for studying candidate molecular pathways underlying spontaneous CSR in hybridomas. (elsevier.com)
  • CD40L is discussed in relation to a potential role in supporting B cell tumors and it has been discovered that the molecular defect in the X-linked Hyper-IgM-Syndrome is targeted to the CD40L gene, it is functional involved in B cell hybridomas and chronic lymphocytic leukemia as well as several autoimmune diseases. (diaclone.com)
DR3 Antibody, anti-human | Hybridomas | MACS Antibodies | Products | Miltenyi Biotec | Canada
DR3 Antibody, anti-human | Hybridomas | MACS Antibodies | Products | Miltenyi Biotec | Canada (miltenyibiotec.com)
2019 AACC: Exhibitors
2019 AACC: Exhibitors (events.jspargo.com)
Protocol for Cultureware Preparation with Semi-Solid Methylcellulose Medium for Cell Culture | STEMCELL Technologies
Protocol for Cultureware Preparation with Semi-Solid Methylcellulose Medium for Cell Culture | STEMCELL Technologies (stemcell.com)
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A single WNT enhancer drives specification and regeneration of the Drosophila wing | Nature Communications
A single WNT enhancer drives specification and regeneration of the Drosophila wing | Nature Communications (nature.com)
Recombinant Anti-CD13 antibody [EPR4058] KO Tested (ab108310) | Abcam
Recombinant Anti-CD13 antibody [EPR4058] KO Tested (ab108310) | Abcam (abcam.com)
EvoPAD - Merve Çalişkan
EvoPAD - Merve Çalişkan (uni-muenster.de)
Mobilan: a recombinant adenovirus carrying Toll-like receptor 5 self-activating cassette for cancer immunotherapy | Oncogene
Mobilan: a recombinant adenovirus carrying Toll-like receptor 5 self-activating cassette for cancer immunotherapy | Oncogene (nature.com)
Bispecific antibodies: a mechanistic review of the pipeline | Nature Reviews Drug Discovery
Bispecific antibodies: a mechanistic review of the pipeline | Nature Reviews Drug Discovery (nature.com)
Plus it
Plus it (jneurosci.org)
Kinetic Analysis of Antibodies from Different Cultured Media
Kinetic Analysis of Antibodies from Different Cultured Media (biopharminternational.com)
Live-cell imaging reveals enhancer-dependent Sox2 transcription in the absence of enhancer proximity | eLife
Live-cell imaging reveals enhancer-dependent Sox2 transcription in the absence of enhancer proximity | eLife (elifesciences.org)
2021 AACC: Exhibitors
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2020 AACC: Exhibitors
2020 AACC: Exhibitors (events.jspargo.com)
PerCP/Cyanine5.5 anti-mouse CD16/32 Antibody anti-CD16/32 - 93
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Frontiers | Six Weeks of Low-Load Blood Flow Restricted and High-Load Resistance Exercise Training Produce Similar Increases in...
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FGF ligands in Drosophila have distinct activities required to support cell migration and differentiation | Development | The...
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Anti-CD161, Alexa Fluor 488, Clone: PK136, R D Systems:Antibodies:Primary | Fisher Scientific
Anti-CD161, Alexa Fluor 488, Clone: PK136, R D Systems:Antibodies:Primary | Fisher Scientific (fishersci.com)
B7-H1/PD-L1 Rat anti-Mouse, Clone: 929903, R D Systems 100μg; Unlabeled:Antibodies, | Fisher Scientific
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Human IgG ELISA Antibody Pair Kit | STEMCELL Technologies
Human IgG ELISA Antibody Pair Kit | STEMCELL Technologies (stemcell.com)