Cells artificially created by fusion of activated lymphocytes with neoplastic cells. The resulting hybrid cells are cloned and produce pure MONOCLONAL ANTIBODIES or T-cell products, identical to those produced by the immunologically competent parent cell.
Antibodies produced by a single clone of cells.
The property of antibodies which enables them to react with some ANTIGENIC DETERMINANTS and not with others. Specificity is dependent on chemical composition, physical forces, and molecular structure at the binding site.
Sites on an antigen that interact with specific antibodies.
Lymphocytes responsible for cell-mediated immunity. Two types have been identified - cytotoxic (T-LYMPHOCYTES, CYTOTOXIC) and helper T-lymphocytes (T-LYMPHOCYTES, HELPER-INDUCER). They are formed when lymphocytes circulate through the THYMUS GLAND and differentiate to thymocytes. When exposed to an antigen, they divide rapidly and produce large numbers of new T cells sensitized to that antigen.
That region of the immunoglobulin molecule that varies in its amino acid sequence and composition, and comprises the binding site for a specific antigen. It is located at the N-terminus of the Fab fragment of the immunoglobulin. It includes hypervariable regions (COMPLEMENTARITY DETERMINING REGIONS) and framework regions.
The largest of polypeptide chains comprising immunoglobulins. They contain 450 to 600 amino acid residues per chain, and have molecular weights of 51-72 kDa.
Lymphoid cells concerned with humoral immunity. They are short-lived cells resembling bursa-derived lymphocytes of birds in their production of immunoglobulin upon appropriate stimulation.
Large, transmembrane, non-covalently linked glycoproteins (alpha and beta). Both chains can be polymorphic although there is more structural variation in the beta chains. The class II antigens in humans are called HLA-D ANTIGENS and are coded by a gene on chromosome 6. In mice, two genes named IA and IE on chromosome 17 code for the H-2 antigens. The antigens are found on B-lymphocytes, macrophages, epidermal cells, and sperm and are thought to mediate the competence of and cellular cooperation in the immune response. The term IA antigens used to refer only to the proteins encoded by the IA genes in the mouse, but is now used as a generic term for any class II histocompatibility antigen.
Any discrete, presumably solitary, mass of neoplastic PLASMA CELLS either in BONE MARROW or various extramedullary sites.
Fusion of somatic cells in vitro or in vivo, which results in somatic cell hybridization.
Any cell, other than a ZYGOTE, that contains elements (such as NUCLEI and CYTOPLASM) from two or more different cells, usually produced by artificial CELL FUSION.
Local surface sites on antibodies which react with antigen determinant sites on antigens (EPITOPES.) They are formed from parts of the variable regions of FAB FRAGMENTS.
Serological reactions in which an antiserum against one antigen reacts with a non-identical but closely related antigen.
Polypeptide chains, consisting of 211 to 217 amino acid residues and having a molecular weight of approximately 22 kDa. There are two major types of light chains, kappa and lambda. Two Ig light chains and two Ig heavy chains (IMMUNOGLOBULIN HEAVY CHAINS) make one immunoglobulin molecule.
Unique genetically-controlled determinants present on ANTIBODIES whose specificity is limited to a single group of proteins (e.g., another antibody molecule or an individual myeloma protein). The idiotype appears to represent the antigenicity of the antigen-binding site of the antibody and to be genetically codetermined with it. The idiotypic determinants have been precisely located to the IMMUNOGLOBULIN VARIABLE REGION of both immunoglobin polypeptide chains.
Genes encoding the different subunits of the IMMUNOGLOBULINS, for example the IMMUNOGLOBULIN LIGHT CHAIN GENES and the IMMUNOGLOBULIN HEAVY CHAIN GENES. The heavy and light immunoglobulin genes are present as gene segments in the germline cells. The completed genes are created when the segments are shuffled and assembled (B-LYMPHOCYTE GENE REARRANGEMENT) during B-LYMPHOCYTE maturation. The gene segments of the human light and heavy chain germline genes are symbolized V (variable), J (joining) and C (constant). The heavy chain germline genes have an additional segment D (diversity).
One of the types of light chains of the immunoglobulins with a molecular weight of approximately 22 kDa.
Morphologic alteration of small B LYMPHOCYTES or T LYMPHOCYTES in culture into large blast-like cells able to synthesize DNA and RNA and to divide mitotically. It is induced by INTERLEUKINS; MITOGENS such as PHYTOHEMAGGLUTININS, and by specific ANTIGENS. It may also occur in vivo as in GRAFT REJECTION.
Molecules on the surface of T-lymphocytes that recognize and combine with antigens. The receptors are non-covalently associated with a complex of several polypeptides collectively called CD3 antigens (ANTIGENS, CD3). Recognition of foreign antigen and the major histocompatibility complex is accomplished by a single heterodimeric antigen-receptor structure, composed of either alpha-beta (RECEPTORS, ANTIGEN, T-CELL, ALPHA-BETA) or gamma-delta (RECEPTORS, ANTIGEN, T-CELL, GAMMA-DELTA) chains.
A group of genetically identical cells all descended from a single common ancestral cell by mitosis in eukaryotes or by binary fission in prokaryotes. Clone cells also include populations of recombinant DNA molecules all carrying the same inserted sequence. (From King & Stansfield, Dictionary of Genetics, 4th ed)
Antigens on surfaces of cells, including infectious or foreign cells or viruses. They are usually protein-containing groups on cell membranes or walls and may be isolated.
Proteins, protein complexes, or glycoproteins secreted by suppressor T-cells that inhibit either subsequent T-cells, B-cells, or other immunologic phenomena. Some of these factors have both histocompatibility (I-J) and antigen-specific domains which may be linked by disulfide bridges. They can be elicited by haptens or other antigens and may be mass-produced by hybridomas or monoclones in the laboratory.
Genetically identical individuals developed from brother and sister matings which have been carried out for twenty or more generations, or by parent x offspring matings carried out with certain restrictions. All animals within an inbred strain trace back to a common ancestor in the twentieth generation.
An immunoassay utilizing an antibody labeled with an enzyme marker such as horseradish peroxidase. While either the enzyme or the antibody is bound to an immunosorbent substrate, they both retain their biologic activity; the change in enzyme activity as a result of the enzyme-antibody-antigen reaction is proportional to the concentration of the antigen and can be measured spectrophotometrically or with the naked eye. Many variations of the method have been developed.
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
Established cell cultures that have the potential to propagate indefinitely.
An encapsulated lymphatic organ through which venous blood filters.
A soluble substance elaborated by antigen- or mitogen-stimulated T-LYMPHOCYTES which induces DNA synthesis in naive lymphocytes.
A class of immunoglobulin bearing mu chains (IMMUNOGLOBULIN MU-CHAINS). IgM can fix COMPLEMENT. The name comes from its high molecular weight and originally being called a macroglobulin.
Allelic variants of the immunoglobulin light chains (IMMUNOGLOBULIN LIGHT CHAINS) or heavy chains (IMMUNOGLOBULIN HEAVY CHAINS) encoded by ALLELES of IMMUNOGLOBULIN GENES.
The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.
T-cell enhancement of the B-cell response to thymic-dependent antigens.
The processes triggered by interactions of ANTIBODIES with their ANTIGENS.
The major immunoglobulin isotype class in normal human serum. There are several isotype subclasses of IgG, for example, IgG1, IgG2A, and IgG2B.
Substances that are recognized by the immune system and induce an immune reaction.
The class of heavy chains found in IMMUNOGLOBULIN M. They have a molecular weight of approximately 72 kDa and they contain about 57 amino acid residues arranged in five domains and have more oligosaccharide branches and a higher carbohydrate content than the heavy chains of IMMUNOGLOBULIN G.
The complex formed by the binding of antigen and antibody molecules. The deposition of large antigen-antibody complexes leading to tissue damage causes IMMUNE COMPLEX DISEASES.
A heterogeneous group of immunocompetent cells that mediate the cellular immune response by processing and presenting antigens to the T-cells. Traditional antigen-presenting cells include MACROPHAGES; DENDRITIC CELLS; LANGERHANS CELLS; and B-LYMPHOCYTES. FOLLICULAR DENDRITIC CELLS are not traditional antigen-presenting cells, but because they hold antigen on their cell surface in the form of IMMUNE COMPLEXES for B-cell recognition they are considered so by some authors.
The genetic region which contains the loci of genes which determine the structure of the serologically defined (SD) and lymphocyte-defined (LD) TRANSPLANTATION ANTIGENS, genes which control the structure of the IMMUNE RESPONSE-ASSOCIATED ANTIGENS, HUMAN; the IMMUNE RESPONSE GENES which control the ability of an animal to respond immunologically to antigenic stimuli, and genes which determine the structure and/or level of the first four components of complement.
A hapten capable of eliciting both antibody formation and delayed hypersensitivity when bound to aromatic amino acids, polypeptides or proteins. It is used as an immunologic research tool.
A measure of the binding strength between antibody and a simple hapten or antigen determinant. It depends on the closeness of stereochemical fit between antibody combining sites and antigen determinants, on the size of the area of contact between them, and on the distribution of charged and hydrophobic groups. It includes the concept of "avidity," which refers to the strength of the antigen-antibody bond after formation of reversible complexes.
The major group of transplantation antigens in the mouse.
Genetic loci in the vertebrate major histocompatibility complex that encode polymorphic products which control the immune response to specific antigens. The genes are found in the HLA-D region in humans and in the I region in mice.
The phenomenon of immense variability characteristic of ANTIBODIES. It enables the IMMUNE SYSTEM to react specifically against the essentially unlimited kinds of ANTIGENS it encounters. Antibody diversity is accounted for by three main theories: (1) the Germ Line Theory, which holds that each antibody-producing cell has genes coding for all possible antibody specificities, but expresses only the one stimulated by antigen; (2) the Somatic Mutation Theory, which holds that antibody-producing cells contain only a few genes, which produce antibody diversity by mutation; and (3) the Gene Rearrangement Theory, which holds that antibody diversity is generated by the rearrangement of IMMUNOGLOBULIN VARIABLE REGION gene segments during the differentiation of the ANTIBODY-PRODUCING CELLS.
Antibodies that react with self-antigens (AUTOANTIGENS) of the organism that produced them.
T-cell receptors composed of CD3-associated alpha and beta polypeptide chains and expressed primarily in CD4+ or CD8+ T-cells. Unlike immunoglobulins, the alpha-beta T-cell receptors recognize antigens only when presented in association with major histocompatibility (MHC) molecules.
The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.
Derivatives of phenylacetic acid. Included under this heading are a variety of acid forms, salts, esters, and amides that contain the benzeneacetic acid structure. Note that this class of compounds should not be confused with derivatives of phenyl acetate, which contain the PHENOL ester of ACETIC ACID.
Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
The process by which antigen is presented to lymphocytes in a form they can recognize. This is performed by antigen presenting cells (APCs). Some antigens require processing before they can be recognized. Antigen processing consists of ingestion and partial digestion of the antigen by the APC, followed by presentation of fragments on the cell surface. (From Rosen et al., Dictionary of Immunology, 1989)
A neoplasm originating from thymic tissue, usually benign, and frequently encapsulated. Although it is occasionally invasive, metastases are extremely rare. It consists of any type of thymic epithelial cell as well as lymphocytes that are usually abundant. Malignant lymphomas that involve the thymus, e.g., lymphosarcoma, Hodgkin's disease (previously termed granulomatous thymoma), should not be regarded as thymoma. (From Stedman, 25th ed)
Proteins secreted by the prostate gland. The major secretory proteins from the human prostate gland include PROSTATE-SPECIFIC ANTIGEN, prostate-specific acid phosphatase, prostate-specific membrane antigen, and prostate-specific protein-94.
One of the types of light chain subunits of the immunoglobulins with a molecular weight of approximately 22 kDa.
Benzene derivatives which are substituted with three nitro groups in any position.
Ordered rearrangement of B-lymphocyte variable gene regions coding for the kappa or lambda IMMUNOGLOBULIN LIGHT CHAINS, thereby contributing to antibody diversity. It occurs during the second stage of differentiation of the IMMATURE B-LYMPHOCYTES.
The production of ANTIBODIES by proliferating and differentiated B-LYMPHOCYTES under stimulation by ANTIGENS.
Deliberate stimulation of the host's immune response. ACTIVE IMMUNIZATION involves administration of ANTIGENS or IMMUNOLOGIC ADJUVANTS. PASSIVE IMMUNIZATION involves administration of IMMUNE SERA or LYMPHOCYTES or their extracts (e.g., transfer factor, immune RNA) or transplantation of immunocompetent cell producing tissue (thymus or bone marrow).
Soluble protein factors generated by activated lymphocytes that affect other cells, primarily those involved in cellular immunity.
The classes of immunoglobulins found in any species of animal. In man there are nine classes that migrate in five different groups in electrophoresis; they each consist of two light and two heavy protein chains, and each group has distinguishing structural and functional properties.
Autoantibodies directed against various nuclear antigens including DNA, RNA, histones, acidic nuclear proteins, or complexes of these molecular elements. Antinuclear antibodies are found in systemic autoimmune diseases including systemic lupus erythematosus, Sjogren's syndrome, scleroderma, polymyositis, and mixed connective tissue disease.
Antibodies which react with the individual structural determinants (idiotopes) on the variable region of other antibodies.
Glycoproteins expressed on all mature T-cells, thymocytes, and a subset of mature B-cells. Antibodies specific for CD5 can enhance T-cell receptor-mediated T-cell activation. The B-cell-specific molecule CD72 is a natural ligand for CD5. (From Abbas et al., Cellular and Molecular Immunology, 2d ed, p156)
Abnormal immunoglobulins characteristic of MULTIPLE MYELOMA.
Antibodies elicited in a different species from which the antigen originated. These antibodies are directed against a wide variety of interspecies-specific antigens, the best known of which are Forssman, Hanganutziu-Deicher (H-D), and Paul-Bunnell (P-B). Incidence of antibodies to these antigens--i.e., the phenomenon of heterophile antibody response--is useful in the serodiagnosis, pathogenesis, and prognosis of infection and latent infectious states as well as in cancer classification.
Endogenous tissue constituents that have the ability to interact with AUTOANTIBODIES and cause an immune response.
Multi-subunit proteins which function in IMMUNITY. They are produced by B LYMPHOCYTES from the IMMUNOGLOBULIN GENES. They are comprised of two heavy (IMMUNOGLOBULIN HEAVY CHAINS) and two light chains (IMMUNOGLOBULIN LIGHT CHAINS) with additional ancillary polypeptide chains depending on their isoforms. The variety of isoforms include monomeric or polymeric forms, and transmembrane forms (B-CELL ANTIGEN RECEPTORS) or secreted forms (ANTIBODIES). They are divided by the amino acid sequence of their heavy chains into five classes (IMMUNOGLOBULIN A; IMMUNOGLOBULIN D; IMMUNOGLOBULIN E; IMMUNOGLOBULIN G; IMMUNOGLOBULIN M) and various subclasses.
A basic enzyme that is present in saliva, tears, egg white, and many animal fluids. It functions as an antibacterial agent. The enzyme catalyzes the hydrolysis of 1,4-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrin. EC 3.2.1.17.
Proteins, glycoprotein, or lipoprotein moieties on surfaces of tumor cells that are usually identified by monoclonal antibodies. Many of these are of either embryonic or viral origin.
Substances elaborated by viruses that have antigenic activity.
Ordered rearrangement of B-lymphocyte variable gene regions coding for the IMMUNOGLOBULIN CHAINS, thereby contributing to antibody diversity. It occurs during the differentiation of the IMMATURE B-LYMPHOCYTES.

Repertoire of human antibodies against the polysaccharide capsule of Streptococcus pneumoniae serotype 6B. (1/2670)

We examined the repertoire of antibodies to Streptococcus pneumoniae 6B capsular polysaccharide induced with the conventional polysaccharide vaccine in adults at the molecular level two ways. In the first, we purified from the sera of seven vaccinees antipneumococcal antibodies and determined their amino acid sequences. Their VH regions are mainly the products of VH3 family genes (candidate genes, 3-23, 3-07, 3-66, and 3-74), but the product of a VH1 family gene (candidate gene, 1-03) is occasionally used. All seven individuals have small amounts of polyclonal kappa+ antibodies (Vkappa1 to Vkappa4 families), although kappa+ antibodies are occasionally dominated by antibodies formed with the product of the A27 Vkappa gene. In contrast, lambda+ anti-6B antibodies are dominated by the antibodies derived from one of 3 very similar Vlambda2 family genes (candidate genes, 2c, 2e, and 2a2) and Clambda1 gene product. The Vlambda2(+) antibodies express the 8.12 idiotype, which is expressed on anti-double-stranded-DNA antibodies. In one case, Vlambda is derived from a rarely expressed Vlambda gene, 10a. In the second approach, we studied a human hybridoma (Dob1) producing anti-6B antibody. Its VH region sequence is closely related to those of the 3-15 VH gene (88% nucleotide homology) and JH4 (92% homology). Its VL region is homologous to the 2a2 Vlambda2 gene (91%) and Jlambda1/Clambda1. Taken together, the V region of human anti-6B antibodies is commonly formed by a VH3 and a Vlambda2 family gene product.  (+info)

Mechanisms of double-strand-break repair during gene targeting in mammalian cells. (2/2670)

In the present study, the mechanism of double-strand-break (DSB) repair during gene targeting at the chromosomal immunoglobulin mu-locus in a murine hybridoma was examined. The gene-targeting assay utilized specially designed insertion vectors genetically marked in the region of homology to the chromosomal mu-locus by six diagnostic restriction enzyme site markers. The restriction enzyme markers permitted the contribution of vector-borne and chromosomal mu-sequences in the recombinant product to be determined. The use of the insertion vectors in conjunction with a plating procedure in which individual integrative homologous recombination events were retained for analysis revealed several important features about the mammalian DSB repair process:The presence of the markers within the region of shared homology did not affect the efficiency of gene targeting. In the majority of recombinants, the vector-borne marker proximal to the DSB was absent, being replaced with the corresponding chromosomal restriction enzyme site. This result is consistent with either formation and repair of a vector-borne gap or an "end" bias in mismatch repair of heteroduplex DNA (hDNA) that favored the chromosomal sequence. Formation of hDNA was frequently associated with gene targeting and, in most cases, began approximately 645 bp from the DSB and could encompass a distance of at least 1469 bp. The hDNA was efficiently repaired prior to DNA replication. The repair of adjacent mismatches in hDNA occurred predominantly on the same strand, suggesting the involvement of a long-patch repair mechanism.  (+info)

The molecular basis of multiple vector insertion by gene targeting in mammalian cells. (3/2670)

Gene targeting using sequence insertion vectors generally results in integration of one copy of the targeting vector generating a tandem duplication of the cognate chromosomal region of homology. However, occasionally the target locus is found to contain >1 copy of the integrated vector. The mechanism by which the latter recombinants arise is not known. In the present study, we investigated the molecular basis by which multiple vectors become integrated at the chromosomal immunoglobulin mu locus in a murine hybridoma. To accomplish this, specially designed insertion vectors were constructed that included six diagnostic restriction enzyme markers in the Cmu region of homology to the target chromosomal mu locus. This enabled contributions by the vector-borne and chromosomal Cmu sequences at the recombinant locus to be ascertained. Targeted recombinants were isolated and analyzed to determine the number of vector copies integrated at the chromosomal immunoglobulin mu locus. Targeted recombinants identified as bearing >1 copy of the integrated vector resulted from a Cmu triplication formed by two vector copies in tandem. Examination of the fate of the Cmu region markers suggested that this class of recombinant was generated predominantly, if not exclusively, by two targeted vector integration events, each involving insertion of a single copy of the vector. Both vector insertion events into the chromosomal mu locus were consistent with the double-strand-break repair mechanism of homologous recombination. We interpret our results, taken together, to mean that a proportion of recipient cells is in a predetermined state that is amenable to targeted but not random vector integration.  (+info)

Infrequent translation of a nonsense codon is sufficient to decrease mRNA level. (4/2670)

In many organisms nonsense mutations decrease the level of mRNA. In the case of mammalian cells, it is still controversial whether translation is required for this nonsense-mediated RNA decrease (NMD). Although previous analyzes have shown that conditions that impede translation termination at nonsense codons also prevent NMD, the residual level of termination was unknown in these experiments. Moreover, the conditions used to impede termination might also have interfered with NMD in other ways. Because of these uncertainties, we have tested the effects of limiting translation of a nonsense codon in a different way, using two mutations in the immunoglobulin mu heavy chain gene. For this purpose we exploited an exceptional nonsense mutation at codon 3, which efficiently terminates translation but nonetheless maintains a high level of mu mRNA. We have shown 1) that translation of Ter462 in the double mutant occurs at only approximately 4% the normal frequency, and 2) that Ter462 in cis with Ter3 can induce NMD. That is, translation of Ter462 at this low (4%) frequency is sufficient to induce NMD.  (+info)

Soluble class I MHC with beta2-microglobulin covalently linked peptides: specific binding to a T cell hybridoma. (5/2670)

Soluble forms of the mouse MHC class I molecule, Dd, were produced in which the peptide binding groove was uniformly occupied by peptides attached via a covalent flexible peptide linker to the N terminus of the associated beta2-microglobulin. The MHC heavy chain and beta2-microglobulin were firmly associated, and the molecules displayed an Ab epitope requiring proper occupancy of the peptide binding groove. Soluble Dd containing a covalent version of a well-characterized Dd-binding peptide from HIV stimulated a T cell hybridoma specific for this combination. Furthermore, a tetravalent version of this molecule bound specifically with apparent high avidity to this hybridoma.  (+info)

Cloning, expression, and characterization of the Fab fragment of the anti-lysozyme antibody HyHEL-5. (6/2670)

Hybridoma cDNAs encoding the individual chains of the Fab fragment of the well characterized murine monoclonal antibody HyHEL-5 were cloned and sequenced. The recombinant Fab fragment was produced by expressing each chain in a separate Escherichia coli pET vector, denaturing inclusion bodies and co-refolding. Characterization of the purified Fab by MALDI-TOF mass spectrometry and N-terminal amino acid sequencing demonstrated proper processing of the individual chains. The association of the recombinant Fab fragment with hen egg lysozyme and the avian epitope variant bobwhite quail lysozyme was found by isothermal titration calorimetry to have energetics very similar to that of the HyHEL-5 IgG. Heterologous expression of the HyHEL-5 Fab fragment opens the way to structure/function studies in this well-known system.  (+info)

A novel 62-kilodalton egg antigen from Schistosoma mansoni induces a potent CD4(+) T helper cell response in the C57BL/6 mouse. (7/2670)

In infection with Schistosoma mansoni, hepatic granuloma formation is mediated by CD4(+) T helper (Th) cells sensitized to schistosomal egg antigens. There is considerable variation among infected individuals with respect to both severity of disease and the T-cell response to egg antigens. In the BL/6 mouse, the egg granulomas are relatively small and the relevant sensitizing egg antigens are largely unknown. We investigated the CD4(+) Th cell response of infected BL/6 mice to egg antigens fractionated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and found a prominent lymphoproliferative response to be directed against a 62-kDa component. With the aid of a specific T-cell hybridoma, 4E6, the 62-kDa antigen was isolated; following partial digestion with endoproteinase Glu-C, an internal amino acid sequence was found to be identical with one present in the enzyme phosphoenolpyruvate carboxykinase (PEPCK) of the organisms Caenorhabditis elegans and Treponema pallidum and to differ by one residue from PEPCK of various other species. In CD4(+) Th cells from 7.5- 8.5-week-infected BL/6 mice, the purified 62-kDa molecule elicited a potent proliferative response which, based on cytokine analysis, was of a mixed Th-1 and Th-2 type. Our results reveal a novel egg antigen of particular prominence in the BL/6 mouse and suggest that the immune response in schistosomiasis is a product of sensitization to egg antigens that may vary considerably in immunogenicity from strain to strain.  (+info)

Immunosuppressant deoxyspergualin-induced inhibition of cell proliferation is accompanied with an enhanced reduction of tetrazolium salt. (8/2670)

Deoxyspergualin (DSG) has both antitumor and immunosuppressive activities. We explored the mechanism of DSG activities using an aqueous soluble analogue, methyldeoxyspergualin (MeDSG) for in vitro culture studies. It is known that DSG has inhibitory effects on cell proliferation, and we also observed that MeDSG inhibited [3H]-thymidine incorporation by rapidly dividing murine T cell hybridomas. However, when tetrazolium (MTT) colorimetric assay was adopted to evaluate its inhibitory effects on cell proliferation, MeDSG induced an enhanced MTT reduction. When we examined whether these results were applicable to the actively dividing cells of other origins than T cells, similar effects were seen with Raji cells, J774.1 cells and NIH3T3 cells. N-30, another analogue which was capable of suppressing anti-SRBC antibody production in vivo, also induced inhibition of cell growth and an enhanced MTT reduction. In contrast, the analogue which failed to prevent the antibody production, neither enhanced MTT reduction nor inhibited cell proliferation. Our results demonstrated that the ability to generate MTT formazan in dividing cells is a common property among, DSG analogue with the immunosuppressive and antiproliferative activities.  (+info)

Murine T cell hybridomas bearing receptors for antigen plus I region gene products were used as immunogens in mice in an effort to raise anti-receptor antisera. The antisera were assayed for anti-receptor activity by the ability to inhibit interleukin 2 production by the T cell hybridomas stimulated by antigen and I region expressing antigen-presenting cells. The T cell hybridomas used in these experiments were made by fusing antigen-specific, I region-restricted BALB/c T cell blasts to the AKR thymoma, BW5147. Three groups of mice were immunized with the T cell hybridomas: (BALB/c X AKR)F1 animals, syngeneic to the hybridoma; (BALB.B X aKR)F1 animals, differing from the hybridomas at H2; and (C.B20 X AKR)F1 animals, differing from the hybridomas at Igh. Mice were immunized multiple times and sera from individual animals were assayed for anti-receptor antibodies. In all groups, some mice produced anti-receptor antibodies by the criterion that they were inhibitory in the assay mentioned above. ...
TY - ABST. T1 - Are defects in the innate immune defense contributing factors for mental disorders?. AU - Foldager, Leslie. AU - Steffensen, Rudi Nora. AU - Thiel, Steffen. AU - Als, Thomas Damm. AU - Kristensen, Ann Suhl. AU - Straarup, Steen. AU - Nielsen, Hans Jørgen. AU - Nordentoft, Merete. AU - Mortensen, Preben Bo. AU - Rosenberg, Raben. AU - Mors, Ole. AU - Jensenius, Jens Christian. PY - 2009. Y1 - 2009. M3 - Conference abstract in proceedings. SP - 107. BT - PhD Day 2009 Abstract Book. Y2 - 16 January 2009 through 16 January 2009. ER - ...
Hybridoma-derived idiotype vaccines have been used for the experimental treatment of human lymphoma over the last twenty years, providing evidence of biological efficacy, clinical efficacy and clinical benefit. However, the product that has come closer to regulatory approval is unlikely to clear that hurdle due to the insufficiently robust data obtained in a recently closed clinical trial. This review aims at discussing the reasons for hybridoma-derived idiotype vaccines, more difficult to produce but also more successful than recombinant idiotype vaccines so far, are unlikely to gain regulatory approval. In particular, it is necessary to examine the many peculiar features of this therapeutic approach in a broader context, with special attention to concepts like customized active immunotherapy and randomization. Most published trials based on hybridoma-derived idiotype vaccines are being analyzed, together with the yet non-peer reviewed data from the only randomized study conducted so far with this
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TY - JOUR. T1 - Analysis of T-cell hybridomas with an unusual MHC class II-dependent ligand specificity. AU - Mendiratta, S. K.. AU - Singh, Nagendra. AU - Bal, V.. AU - Rath, S.. PY - 1996/1/1. Y1 - 1996/1/1. N2 - We have characterized two unusual T-cell hybridomas, 1E3 and 3B8, from H-2(k) mice immunized with I-Ab-transfected L cells (H-2(k)), that are stimulated by L cells transfected with I-Ab, I-A(k) or I-Eb, but not by non-transfected L cells. These hybridomas could not be stimulated by spleen cells from H-2(i3), H-2(k), H-2b or H-2(d) mice. Monoclonal anti-I-A antibodies did not block their responses, suggesting that mouse major histocompatibility complex (MHC) class II molecules may be peptide donors rather than restriction elements for them. The stimulation of these hybridomas by fibroblast targets was not blocked by an anti-H-2k(k),D(k)-specific monoclonal antibody. Lipopolysaccharide (LPS)-activated splenic and peritoneal exudate cells from H-2(k), H-2(d), H-2(i3), H-2b as well as ...
TY - JOUR. T1 - Changes of monosaccharide availability of human hybridoma lead to alteration of biological properties of human monoclonal antibody. AU - Tachibana, Hirofumi. AU - Taniguchi, Kiyotaka. AU - Ushio, Yoshitaka. AU - Teruya, Kiichiro. AU - Osada, Kazuhiro. AU - Murakami, Hiroki. PY - 1994/1/1. Y1 - 1994/1/1. N2 - The effect of glucose and other monosaccharide availability in culture medium on production of antibody by human hybridomas has been studied. Human hybridoma cells C5TN produce an anti lung cancer human monoclonal antibody, and the light chain is N-glycosylated at the variable region. When the cell line was grown in the presence of various concentrations of glucose, the antibodies produced changed their antigen-binding activities. Analysis of the light chains produced under these condition revealed that four molecular-mass variant light chains ranging from about 26 to 32 kDa were secreted. The twenty six-kDa species, which corresponds to a non-glycosylated form of the light ...
We have examined the ability of hCD4 to interact functionally with mouse class II MHC molecules using the mouse T cell hybridoma BI-141, specific for beef insulin. We have previously shown that expression of mouse CD4 results in a marked enhancement of IL-2 release by BI-141 cells in response to beef insulin or, in a cross-reactive response, to pork insulin, on the appropriate mouse APCs. We now demonstrate that expression of hCD4 results in an equivalent stimulation of antigen responses by this mouse T cell hybridoma. The specificity of this effect was demonstrated by mAb and gp120 blocking studies. These data provide the first direct evidence for function of hCD4 and in an exclusively mouse system. ...
A murine hybridoma cell line has been established that consistently forms large cytoplasmic inclusions. These structures bind antibody specific for mouse kappa L chain when stained in situ. SDS-PAGE analysis of isolated inclusion bodies produce a single protein band of approximately 26,000 Mr that reacts with anti-kappa antibody when transferred to nitrocellulose. No carbohydrate was detected in association with the purified protein. These data are consistent with the intracellular retention and deposition of complete kappa L chain protein. ...
TY - JOUR. T1 - Detection and characterization of murine ecotropic recombinant virus in myeloma and hybridoma cells. AU - Deo, Y.. AU - Ghebremariam, H.. AU - Cloyd, M.. PY - 1994. Y1 - 1994. N2 - Ecotropic recombinant virus (ERV), a relatively new class of murine retrovirus endogenous to mice, is expressed at significant levels by most murine myeloma and hybridoma cells examined. The routine XC, S+L-, mink cell focus-inducing (MCF), and reverse transcriptase (RT) tests are not suitable to detect and quantify the levels of ERV. A serological focus assay, based on specific anti-murine leukemia virus (MuLV) viral envelope (env) antibodies, is required to detect ERV. A more sensitive format of this serological focus assay includes co-cultivation of test article cells with the indicator (Mus dunni) cells. ERV isolated from murine hybridoma cells show a unique pattern of cross-reactivity with anti-MuLV env antibodies and this pattern is clearly distinct from that of ectropic and xenotropic ...
The bispecific monoclonal antibody (Bi-MAb) HRS-3/AP-1 was developed by somatic hybridization of the 2 mouse hybridoma cell lines HRS-3 and AP-1, which produce monoclonal antibodies with reactivity against the Hodgkins- and Reed-Sternberg cell-associated CD30 antigen and alkaline phosphatase, respectively. After an active incubation with alkaline phosphatase, purified whole immunoglobulin molecules and F(ab′)2 fragments of the Bi-MAb were equally effective in converting a relatively noncytotoxic prodrug, mitomycin phosphate (MOP), into mitomycin alcohol, which was 100 times more toxic to the Hodgkins- and Reed-Sternberg cell line L540 (CD30+) than MOP. The cytotoxic activity of MOP was unaffected when the cells were pretreated with either the Bi-MAb or the enzyme alone. The Bi-MAb HRS-3/AP-1 did not bind to HPB-ALL cells (CD30-) and was not able to activate MOP on these cells. In cocultivation experiments with HPB-ALL and L540 cells, the activation of MOP by the Bi-MAb HRS-3/AP-1 and ...
Kanagawa, O.; Nakauchi, H.; Sekaly, R.P.; Maeda, K.; Takagaki, Y., 1990: Expression and function of the transfected CD8 alpha chain in murine T cell hybridomas
MPs Opti-Clone™ is a partially purified hybridoma growth medium supplement which improves the cloning efficiency of murine B-cell hybridomas. It will enhance the growth of hybridomas cultured at low cell densities, and it will dramatically increase the number of antibody producing colonies during HAT selection. It supports the growth of hybridomas used in the manufacture of monoclonal antibodies. The formulation is suitable for use in cloning and fusion applications. Features: ∙ Promotes hybridoma growth ∙ Eliminates feeder cell layers ∙ Increases antibody production ∙ Improves stressed cells viability ∙ Improves surviving hybridomas ...
Expression of cellular oncogenes was studied in a T cell hybridoma that undergoes cytolytic activation when stimulated by specific antigen or by anti-Thy-1 antibody. The activation occurs without induction of hybridoma proliferation, providing a model to examine oncogene expression during functional differentiation of lymphocytes. We found that c-fos and c-ets-1 mRNAs were transiently induced at high levels in the hybridoma 30 min and 4 h after stimulation, respectively. c-myc and c-ets-2 oncogenes were constitutively expressed in the hybridoma and their mRNA levels were unaffected during 4 h of stimulation, although c-myc expression was reduced in the later stage of stimulation. Inhibitors of T cell activation, cyclosporin A and anti-LFA-1 antibody, blocked the induction of c-fos and c-ets-1 mRNAs without reducing the levels of c-myc and c-ets-2. The results indicate that the functional activation of the CTL hybridoma is associated with induction of c-fos and c-ets-1 genes. ...
The aim of this study was to establish hybridomas capable of long-term production of human monoclonal antibodies (mAbs). Heterohybridization was performed between the mouse myeloma cell line P3X63Ag8.653 and activated human peripheral blood lymphocytes (PBL). In order to achieve better retention of human chromosomes, as well as to improve the stability of the heterohybrids, one HAT-sensitive immunoglobulin (Ig)-non-secreting human x mouse (h x m) heteromyeloma was fused for a second time with activated human PBL. In this way, a panel of HAT-sensitive Ig-non-secreting h x h x m heteromyelomas was obtained and tested for its ability to generate stable human Ig-secreting heterohybrids with activated human PBL. Six lines were selected on the basis of their enhanced characteristics of fusion efficiency and genetic stability. When fused with in vitro immunized human PBL, they generated several h x h x h x m hybridomas stably secreting high yields (10-23 micrograms/ml/24 h) of human mAbs reactive with ...
TY - JOUR. T1 - Transmural pressure induces IL-6 secretion by intestinal epithelial cells. AU - Kishikawa, H.. AU - Miura, S.. AU - Yoshida, H.. AU - Hirokawa, M.. AU - Nakamizo, H.. AU - Higuchi, H.. AU - Adachi, M.. AU - Nakatsumi, R. C.. AU - Suzuki, H.. AU - Saito, H.. AU - Ishii, H.. N1 - Copyright: Copyright 2008 Elsevier B.V., All rights reserved.. PY - 2002. Y1 - 2002. N2 - Although intestinal epithelial cells (IECs) are known as an important source for IL-6, it is not known whether mechanical forces affect IL-6 production. We investigated how transmural pressure modulates IL-6 synthesis and activation of transcription factors in IECs. Pressure was loaded onto IEC-18 cells by introducing compressed helium gas into the cell culture flask for 1-48 h. IL-6 release into the culture media was determined by cell proliferation bioassay using an IL-6-dependent mouse hybridoma cell line (7TD1). Exposure to pressure (80mmHg) significantly enhanced IL-6 release into the culture media from IEC-18 ...
article{9a5c9918-f28d-42f7-9e4a-e617d47af020, abstract = {Development of type-II collagen (CII)-induced arthritis (CIA) is dependent on a T-cell mediated activation of autoreactive B cells. However, it is still unclear if B cells can present CII to T cells. To investigate the role of B cells as antigen-presenting cells (APCs) for CII, we purified B cells from lymph nodes of immunized and nonimmunized mice. These B cells were used as APC for antigen-specific T-cell hybridomas. B cells from naïve mice did present native, triple-helical, CII (nCII) but also ovalbumin (OVA) and denatured CII (dCII) to antigen-specific T-cell hybridomas. In addition, B cells primed with nCII or OVA, but not dCII, activated the antigen-specific T-cell hybridomas two to three times better than naïve B cells. We conclude that antigen-primed B cells have the capacity to process and present CII to primed T cells, and antigen-primed antigen-specific B cells are more efficient as APC than naïve B cells. We further ...
Read PDF to know how XP Media & CloneMedia for Mouse Hybridoma Generation provide a complete solution that supports all stages of hybridoma cell line development.
False-colour scanning electron micrograph of a hybridoma cell producing a monoclonal antibody to cytoskeleton protein. Monoclonal antibodies are produced by injecting a mouse with an antigen & harvesting its antibody response by removing the B lymphocytes. These are fused with myeloma (tumour) cells taken from a mutant lymphocyte source. The fusion product is called a hybridoma cell. B lymphocytes are short-lived; when fused with tumour cells, which divide indefinitely, the continuous production of antibody is secured. Hybridomas are screened for the required antibody; they are then cloned & produce that antibody. Magnification: x1500 at 35mm size. - Stock Image G400/0031
Hybridomas are immortalized cells derived from the fusion of B lymphoblasts with a myeloma fusion partner. Some hybridomas in the ATCC collection are somatic cell hybrids. These cells are capable of producing immunoglobulins that are specific for viral, bacterial or cellular targets.
Adaltis can produce purified and not purified antibody by using its cell lines. Antibodies can be purified on request by: HPLC , FPLC , affinity chromatography and gel filtration.. For production service information please contact: [email protected] ...
The variable-region genes of monoclonal antibody against spores were cloned from mouse hybridoma cells by reverse transcription-PCR. food spoilage (9). Control of the bacterial spores in meals processing is vital that you ensure the basic safety and an extended shelf lifestyle of foods. To keep the product quality and basic safety of foods, polyclonal and […]. ...
There is disclosed a polypeptide (CD40-L) and DNA sequences, vectors and transformed host cells useful in providing CD40-L polypeptides. More particularly, this invention provides isolated human and murine CD40-L polypeptides that bind to the extracellular binding region of a CD40 receptor. Also disclosed are methods of simulating hybridoma cells to increase monoclonal antibody production by administering a CD40 ligand polypeptide that stimulates B cell proliferation.
METHODS OF PRODUCING HYBRIDOMAS AND MONOCLONAL ANTIBODIES AND ANTIBODIES PRODUCED THEREBY - diagram, schematic, and image 150 ...
The present invention pertains to the novel hybridoma SDW18.1.1, hybridomas obtained from SDW18.1.1, monoclonal antibodies obtained from such hybridomas and derivatives of such monoclonal antibodies. The novel hybridomas are formed by fusion of cells from a mouse myeloma line and spleen cells from a mouse previously immunized with cachectin/TNF. Diagnostic and therapeutic utilities for the monoclonal antibodies and their derivatives are proposed, and testing procedures, materials in kit form and pharmaceutical compositions are likewise set forth.
Anti-ABeta Globulomer Antibodies, Antigen-Binding Moieties Thereof, Corresponding Hybridomas, Nucleic Acids, Vectors, Host Cells, Methods of Producing Said Antibodies, Compositions Comprising Said Antibodies, Uses Of Said Antibodies And Methods Of Using Said Antibodies - diagram, schematic, and image 78 ...
The DO11.10 mouse model is a valuable tool for studies of T cell immigration, immunoregulation, development, activation, and function. The KJ1-26 monoclonal antibody reacts with the T cell receptor (TCR) expressed on lymphocytes of the DO11.10 transgenic mouse and the TCR of the BALB/c-derived DO11.10 and DO11.10.24 T cell hybridoma. The DO11.10 TCR is specific for the chicken ovalbumin (OVA) peptide (323-339) in the context of I-A[d] major histocompatibility (MHC) molecules. Transgenic DO11.10 T cells also recognize OVA peptide from jungle fowl and turkey in the presence of A20-1.11, the H-2d-bearing, antigen-presenting B cell lymphoma. - Danmark
In a previous article, RAMPITSCH at BCRSSU.AGR.CA wrote: ,Hello nets: ,Im not sure if this is the right place to ask, but ..... ,I have a hybridoma cell line contaminated with fibroblasts. Normally I dont ,have a problem eliminating these since they stick to the plate a lot better ,than the hybridomas do: simply transferring the cells to a new plate usually ,does the trick. Now I have a line of fibroblasts which refuses to die (even ,after two months and more transfers than I care to remember). The hybridoma ,cell line (needless to say an important one) is growing poorly as it is barely ,able to attach to the plate and cant compete with these aggressive fibro- ,blasts (maybe they have been transformed?). Is there a miracle anti-fibro- ,blast agent? Is there any way of selectively getting rid of these fibroblasts? , ,Thanks for any help and comments, ,Chris R. , Chris: I may be brain-cramping but cant you just sort out the hybridomas? Or even pan them? Use something like an anti-Ig-FITC ...
Gibbons, J J.; Kim, Y T.; and Siskind, G W., Regulation of hybridoma antibody production by coculture with antigen specific or idiotype specific immune spleen cell. Abstr. (1982). Subject Strain Bibliography 1982. 1805 ...
All rights reserved. No part of this book may be reproduced in any form by any electronic or mechanical means without permission in writing from the author ...
Hybridoma cells usually grow to fairly low cell densities in batch cultures (1-3×106 cells/ml). The reason for this is either that essential nutritional components of the medium are consumed, or that
1. It is the disease caused by the novel coronavirus. Patients may have fever with respiratory symptoms - cough, runny nose, sore throat or difficulty breathing. It can even be fatal if the symptoms are severe.. 2. Route of Transmission: Human-Human transmission through droplets from coughing and sneezing of infected person and close contact with COVID-19 cases.. 3. Incubation period: 2-14 days. ...
An international team has developed an AI algorithm with social skills that has outperformed humans in the ability to cooperate with people and machines in playing a variety of two-player games. The researchers, led by Iyad Rahwan, PhD, an MIT Associate Professor of Media Arts and Sciences, tested humans and the algorithm, called S# (S sharp), in three types of interactions: machine-machine, human-machine, and human-human. In most instances, machines programmed with… read more. ...
An international team has developed an AI algorithm with social skills that has outperformed humans in the ability to cooperate with people and machines in playing a variety of two-player games. The researchers, led by Iyad Rahwan, PhD, an MIT Associate Professor of Media Arts and Sciences, tested humans and the algorithm, called S# (S sharp), in three types of interactions: machine-machine, human-machine, and human-human. In most instances, machines programmed with… read more. ...
The Gothenburg-based artist Nino Mick suggests in one of their poems that ultimately biology is our only home. The film programme suggests different ways of approaching such an understanding of biology as home. This film programme consists of a screening of two movies: Pojktanten and a short film/installation, Sporing Lips of Transposed Desire. Each of these two audio-visual artefacts explore floral-aesthetics through something that can be rendered as plant-human intimacies. The programme is inviting the audience as entrants - more than simply viewers - to engage in these ecological intimacies and to think of the ethics and potentials of thinking intimacies as beyond a human-human relationality. Approaching the films through such an angle of ecological intimacy might allow to perceive a decentred understanding of the human through multiplicities and entanglements with others. Wibke will offer in a short introductory talk to the programme as a thinking tool to apply to the film ...
During the therapy session, visual 3D motion tracking will be used via Kinect, audio will be recorded, and basic measurements (i.e. the distance between a participants wrist and elbow) will be taken. After the patient-therapist interaction has been mapped, members of the research team will build computer based models of the therapist and patient using the insights gained from the human-human interaction. The models developed in this proposed study will be used as a template for programming safe and intuitive humanoid-patient interactions for future study. The model of the therapist will be implemented on the Robot (w/o a patient involved) and in a simulation environment where it will be tested with a computer-based model of the patient. ...
mouse anti-estrogen receptor alpha, ligand binding domain (aa 304-554) hybridoma (ERalpha BZ1) is an eagle-i resource of type Hybridoma cell line at eagle-i Network Shared Resource Repository.
mouse anti-mineralocorticoid receptor hybridoma (rMR365 4D6) is an eagle-i resource of type Hybridoma cell line at eagle-i Network Shared Resource Repository.
The T cell receptor (TCR) is a heterodimer composed of two transmembrane glycoprotein chains, α and β. Both chains are members of the Ig superfamily and consist of a constant and a polymorphic variable region. The variable region of the TCRα/β is involved in recognition of antigenic peptides presented by the MHC complex of antigen-presenting cells. The Anti-TCRα/β antibody recognizes a common determinant of the TCRα/β-CD3 complex. - Lëtzebuerg
After obtaining either a media sample of cultured hybridomas or a sample of ascites fluid, the desired antibodies must be extracted. Cell culture sample contaminants consist primarily of media components such as growth factors, hormones and transferrins. In contrast, the in vivo sample is likely to have host antibodies, proteases, nucleases, nucleic acids and viruses. In both cases, other secretions by the hybridomas such as cytokines may be present. There may also be bacterial contamination and, as a result, endotoxins that are secreted by the bacteria. Depending on the complexity of the media required in cell culture and thus the contaminants, one or the other method (in vivo or in vitro) may be preferable. The sample is first conditioned, or prepared for purification. Cells, cell debris, lipids, and clotted material are first removed, typically by centrifugation followed by filtration with a 0.45 µm filter. These large particles can cause a phenomenon called membrane fouling in later ...
Hybridoma technology is used to fuse fusion a B cell and myeloma to form a hybridoma that produces identical monoclonal antibodies.
TY - JOUR. T1 - Analysis of suppressor T cells induced by donor-specific transfusion (DST). T2 - establishment of a human T cell hybridoma producing an antigen-nonspecific suppressor factor.. AU - Fujiwara, T.. AU - Sakagami, K.. AU - Kusaka, S.. AU - Uda, M.. AU - Orita, K.. PY - 1992. Y1 - 1992. N2 - Formation of suppressor T cells (Ts) induced by donor-specific transfusion (DST) is one of the most commonly suggested mechanisms for the beneficial effect of DST. In this study, we established a human T cell hybridoma derived from the peripheral blood lymphocytes (PBL) of a DST-treated patient, which produced an antigen-nonspecific suppressor factor. Post-DST PBL were fused with an azaguanine-resistant mutant of a human T cell leukemia cell line, CCRF-CEM(AG). After selection and cloning, we established one clone producing the mixed lymphocyte reaction (MLR) inhibitory factor (C524: 18%-43% suppression). Suppressive activity of the supernatant obtained from C524 after activation by PHA was highly ...
Cesar Milstein and Georges J.F. Kohler invented the production of monoclonal antibodies in 1975 for which they got the Noble Prize of 1984 for Medicine and Physiology along with Niels Kaj Jerne, who made other contributions to immunology. The term hybridoma was introduced by Leonard Herzenberg in 1976-1977. Principle: The method starts by injecting a mice with immunogen. The mice should response to immunogenic reaction. A kind of somatic cell, the B-cell, produces antibodies that bind to the immunogen. These recently created antibodies are then collected from the mice. These isolated cells are merged with immortal B-cells (a myeloma cell), in order to provide a hybrid cell known as Hybridoma. These hybridomas have the antibody producing ability of the B-cell and also have the reproducibility and longevity. The hybridomas are fully grown in culture medium. In addition, the manufactured antibodies are all chemically identical in distinct to polyclonal antibody.. Hybridoma Production of Monoclonal ...
The most comprehensive custom mouse monoclonal antibody production services: custom production of monoclonal antibodies against non-modified, phospho-specific, methylation-specific, and acetylation-specific peptides and proteins; monoclonal antibodies for ELISA, Western blot, IP, IF, ICC, and IHC applications. Guaranteed ELISA > 1:40 000.
TY - JOUR. T1 - Monoclonal antibodies to various morphologic components of human skin. AU - Aiba, S.. AU - Masuko, T.. AU - Hosokawa, M.. AU - Hashimoto, Y.. PY - 1983/1/1. Y1 - 1983/1/1. N2 - Somatic cell hybrids were established from the mouse myeloma, P3x63Ag8.653 cells, and the spleen cells of a mouse hyperimmune to human epidermal cells. Indirect immunofluorescence test with hybridoma culture fluids displayed that 253 out of 263 hybridoma cultures secreted antibodies reactive with the frozen sections of human skin. The hybridomas secreting unique antibodies to skin components were cloned and designated as AHS-1 to -8. Monoclonal antibodies (MoAb) from AHS-1, AHS-2, and AHS-3 hybridomas did detect cytoplasmic antigens present in the epidermal layer, eccrine ducts and glands (except MoAb AHS-1), and hair follicles. Enzyme-linked immunosorbent assay showed that the antigen recognized by either MoAb AHS-1 or MoAb AHS-2, but not by MoAb AHS-3, shares the antigenic determinant with antigen(s) ...
Monoclonal antibodies (MAbs) are made by identical immune cells and target one particular epitope by monovalent or monospecific affinity. The high affinity and selective binding of MAbs to epitopes in target antigens makes them highly potent tools for use in biochemistry, molecular biology and medicine. The first working method described for the isolation of monoclonal antibodies was hybridoma technology, based on forming hybrid cell lines (hybridomas) by fusing an antibody-producing B-cell with a myeloma cell [1]. The antibodies produced by a particular hybridoma clone share the same specificity. Thus, individual clones can be screened for the production of an antibody with the desired affinity. However, hybridoma technology has shortcomings: it takes a relatively long time (on the order of months) and has not been widely applied to organisms other than mice. Moreover, antibody sequence information is unavailable by this method. Thus, when a hybridoma-screened antibody is selected for further ...
Our hybridoma bank contains over 20 murine hybridomas. Moreover, we have extensive experience in the purification and characterization of numerous murine monoclonal antibodies for in vitro and in vivo use. We have also generated monoclonal antibodies de novo by in vivo immunization and subsequent in vitro fusion of heterologous fusion partners from Chinese hamsters and rats, and produced IgA-secreting hybridomas de novo. Depending on the quantity of monoclonal antibodies required, hybridomas are propagated in either static flat tissue culture flasks or in roller bottle cultures. Monoclonal antibodies are purified by affinity column chromatography using protein G charged columns. The affinity purified monoclonal antibodies are dialyzed against deionized water, concentrated on a vacuum concentrator, resuspended in sterile PBS, and the protein concentration determined by BCA. The purity of the monoclonal antibodies is confirmed by western blotting with commercial anti-mouse IgG or IgM, antibodies, ...
The MD Anderson Monoclonal Antibody Core Facility provides custom monoclonal antibody production and purification to researchers at MD Anderson and beyond. Learn more.
hi friends ! iam handling few cell lines ! we recently bought Caco-2 cell line. i have ended up mixing caco-2 cells with hybridoma cells and now iam unable to remove this contamination. Hybridoma cell line has a doubling time of just 24hrs is growing fast and soon will replace Caco-2 cells. caco-2 cells are really slow growing cells. i tried few mwthods to isolate them but failed ...
Plasmacytoid dendritic cells (pDCs) are a subpopulation of dendritic cells that secrete large amounts of interferons (IFNs) in response to stimuli that activate the Toll-like receptors TLR9 or TLR7. The C-type lectin blood dendritic cell antigen 2 (BDCA2) is uniquely expressed by pDCs, and antibodies that bind BDCA2 inhibit IFN production in response to TLR signaling. Cao et al. found that the abundance of BDCA2 at the cell surface was increased, and signaling was reconstituted in Jurkat cells or a mouse T cell hybridoma cell line when the cells were also transfected to express the γ subunit of the Fcε receptor (FcεR1γ) but not when BDCA2 was coexpressed with the immunoreceptor tyrosine-based activation motif (ITAM) adaptor DAP12 or the related protein DAP10. Analysis of the mRNA and protein abundance of signaling proteins associated with either B cell receptor (BCR) or T cell receptor (TCR) signaling revealed that the signaling cascade of isolated human pDCs most resembled that downstream ...
Monoclonal Antibody Production Service (Taiwan) Start Order Immediately by Completing the Inquiry Form.. I. Hybridoma fusion and screening service: 16 weeks (USD 5,100). II. MAP Immunogen monoclonal antibody production: Total 19 weeks (USD 5,800). III. LAE antigen monoclonal antibody production: Total 20 weeks (USD 6,600). IV. Optional Services. I Hybridoma fusion and screening service: 16 weeks. ...
Hybridoma technology has been used successfully to generate monoclonal-antibody probes against protoplast membrane antigens. Hybridomas secreting monoclonal antibodies that either inhibit or stimulate a putative plasma-membrane marker enzyme, (K+ + Mg2+)-stimulated pH 6.5 ATPase, have been identified and cloned. The specificity of monoclonal-antibody probes on the activity of other phosphate-hydrolysing enzymes has also been examined. The production and identification of monospecific antibodies capable of immunoreacting with particular component proteins in a complex plant membrane mixture highlight the usefulness of hybridoma methodology for the enzymologist, especially since such monoclonal antibodies can be used in the purification of proteins by immunoaffinity techniques. ...
TY - JOUR. T1 - Modified hybridoma methodology. T2 - Antigen-directed chemically mediated cell fusion. AU - Kranz, David M. AU - Herron, James N.. AU - Billing, Patricia A.. AU - Voss, Edward W.. PY - 1980/1/1. Y1 - 1980/1/1. N2 - The concept of antigen-directed cell fusions to increase the yield of hybridomas was investigated. To facilitate cell-cell contact, antigen conjugated cells were used in cell fusion studies. Specifically, fluorescyl conjugated murine myeloma cells (Sp 2/0-Ag14) incubated with murine immune (anti-fluorescyl) splenocytes formed aggregates containing fluorescent and non-fluorescent cells. Fusion of these populations with polyethylene glycol resulted in a greater number of anti-fluorescyl hybridomas relative to normal fusions under non-antigen directed condition. Ligand binding data indicated that despite the multicellular aggregates the hybridomas resulting from chemically mediated fusions produced only one monoclonal Ig.. AB - The concept of antigen-directed cell fusions ...
Within the functional group of transcriptional regulation, we find the transcription factor Fosl2 (also known as Fra2), a Fos family member, among the most prominently up-regulated by both chemopreventive compounds. Interestingly, an antitumor promoter, the phenolic antioxidant tert-butylhydroquinone, was reported to induce expression of Fra2 (Fosl2) as well as Fra1. Furthermore, the authors concluded that inhibitory activator protein-1 complexes composed of Jun-Fra heterodimers, induced by tert-butylhydroquinone, antagonize the transcriptional effects of the tumor promoter 12-O-tetradecanoylphorbol-13-acetate, which are mediated by Jun-Fos heterodimers (21). Similarly, inhibition of interleukin-6-stimulated cell growth of human myeloma and mouse hybridoma cells was shown to be associated with increased expression of Fra2 protein (22). Fra2 has also been associated with differentiation in epidermis, and exogenous expression of Fra-2 (Fosl2) repressed activator protein-1 transcriptional activity ...
Recent isolates of RX54-3 hybridoma cells (new cells) protect BALB/c mice against subsequent challenge with the tumorigenic myeloma parent cells used to construct this hybridoma. In contrast, hybridoma cells which have been maintained in tissue culture for long periods of time (old cells) are not protective. In the present study, we compared a number of properties of the new and old hybridoma cells and determined which line was more similar to the parent myeloma. We found that new hybridoma cells resembled myeloma cells in: (a) possessing A- and C-type viral particles on transmission electron microscopy and a relatively smooth surface on scanning electron microscopy; (b) being sensitive to a hypotonic solution containing the dye propidium iodide; (c) having similar DNA histograms on flow cytometric analysis; (d) being sensitive to the bacteriocin colicin HSC 10; and (e) being tumorigenic in nude mice. In contrast, old hybridoma cells differed in all of these characteristics from new hybridoma ...
Get this from a library! Hybridomas and monoclonal antibodies : presented at the Thirty-Fourth Annual Meeting of the American Association of Blood Banks, November 3, 1981. [Serafeim P Masouredis;]
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Production and Purification of your monoclonal antibodies (IgG, IgM and more) from Hybridoma or Cell Culture Supernatants produced by Davids or send to us.
The isolation and production of human monoclonal antibodies is becoming an increasingly important pursuit as biopharmaceutical companies migrate their drug pipelines away from small organic molecules. As such, optimization of monoclonal antibody technologies is important, as this is becoming the new rate-limiting step for discovery and development of new pharmaceuticals. The major limitations of this system are the efficiency of isolating hybridoma clones, the process of stabilizing these clones and optimization of hybridoma cell secretion, especially for large-scale production. Many previous studies have demonstrated how perturbations in the aqueous environment can impact upon cell biology. In particular, radio frequency (RF) irradiation of solutions can have dramatic effects on behavior of solutions, cells and in particular membrane proteins, although this effect decays following removal of the RF. Recently, it was shown that nanoparticle doping of RF irradiated water (NPD water) produced a stabilized
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Hybridoma-derived monoclonal antibodies were raised to enterotoxins of the cholera family and to chimeric B-subunit proteins in which individual amino acid residues of a heat-labile, cholera-related enterotoxin from an Escherichia coli strain of porcine origin (P-LT) were substituted with correspond …
September 24-25, 1997 , Baltimore, MD. A workshop of The Johns Hopkins Center for Alternatives to Animal Testing and The Office for Protection from Research Risks, National Institutes of Health ...
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Purpose: : Recruitment of immune cell subsets to the site of transplantation is potentiated in part by primordial chemokines such as CXCL1/KC and later CXCL9/Mig and CXCL10/IP-10, which are markedly up-regulated in high risk (HR) allogeneic corneal transplant recipients but not in syngeneic or normal risk allogeneic recipients. The purpose of this study is to address the effect of newly generated anti-chemokine mAbs as potential therapy for diminishing the influx of inflammatory immune cells into HR corneal allografts, therefore leading to enhanced graft survival. Methods: : Wild type (wt) C57BL/6 (B6), mice were immunized with CXCL1 peptide plus universal T cell epitope (PADRE) as adjuvant and boosted 14 days later with the same combination. Hybridoma lines were created by fusion of splenocytes from immunized mice with SP2/IL-6 myeloma cells. Anti-CXCL1/KC clones were selected based on their ability to bind whole CXCL1/KC by ELISA. Thioglycollate-elicited neutrophils or macrophages from ...
An suitable adjuvant is blended with microgram or milligram amounts of immunogen and injected at multiple site of the mouse at various times in a repetitious manners. At times the mice is bled and examination for antibodies of purposed specificity is made. Highest concentration of antibodies are established after the mouse is immense with 2-3 individual delicate doses of antigen. At the point when concentration of antibody are most extreme the mouse is yielded and the spleen is taken. Then it is separated into individual spleenocyte by applying enzyme or instrumental process. Lymphocytes of the spleen is separated from rest of the organelles by Density gradient centrifugation . ...
Hybridoma technology is used to fuse fusion a B cell and myeloma to form a hybridoma that produces identical monoclonal antibodies.
Antibodies Hybridomas. 3 (2): 65-74. PMID 1633267. Roes J, Rajewsky K (1992). "Cell autonomous expression of IgD is not ...
Hybridoma. 17 (6): 497-507. doi:10.1089/hyb.1998.17.497. PMID 9890705. Pulford K, Jones M, Banham AH, et al. (1999). " ...
Hybridoma. 11 (1): 33-39. doi:10.1089/hyb.1992.11.33. ISSN 0272-457X. Bolstad, Nils; Warren, David J.; Nustad, Kjell (2013-10- ...
1986). "Pancreatic amylase expression in human pancreatic development". Hybridoma. 5 (2): 137-45. doi:10.1089/hyb.1986.5.137. ...
Hybridoma. 17 (5): 431-5. doi:10.1089/hyb.1998.17.431. PMID 9873988. Enright, Helen (Oct 1995). "Paraneoplastic autoimmune ...
Hybridoma. 20 (3): 159-66. doi:10.1089/027245701750293484. PMID 11461664. Yoon HG, Chan DW, Reynolds AB, Qin J, Wong J (Sep ...
Hybridoma. 20 (3): 149-57. doi:10.1089/027245701750293475. PMID 11461663. ...
Hybridoma. 26 (4): 231-40. doi:10.1089/hyb.2007.0507. PMID 17725385. Stecca, Barbara; Mas, Christophe; Altaba, Ariel Ruiz i ( ...
Hybridoma. 20 (3): 149-57. doi:10.1089/027245701750293475. PMID 11461663. Ali L, Flowers SA, Jin C, Bennet EP, Ekwall AK, ...
Hybridoma (Larchmt). 28 (4): 251-7. doi:10.1089/hyb.2009.0017. PMID 19663697. Palumbo KS, Wands JR, Safran H, King T, Carlson ...
Hybridoma 1986; 5:179. 41. Schwarting R, Stein H and Wang CY. monoclonal antibody S-HCL1 and S-HCL3 allow the diagnosis of ... Preparation and characterization of monoclonal antibodies directed against epitopes of Human IFN-y. Hybridoma 1984; 4:321. 45. ...
Hybridoma and Hybridomics. 21 (1): 1-10. doi:10.1089/15368590252917584. PMID 11991811. Bromidge T, Lynas C (Jul 2002). " ...
Hybridomas in the Biosciences and Medicine, 1985. Timothy Springer, ed., Plenum Press, N.Y. p. 397-406. Page BD, Snyder M. CIK1 ...
The National Centre for Cell Science (NCCS), Pune, India; national repository for cell lines/hybridomas etc. Public Health ... to produce a hybridoma which has the antibody specificity of the primary lymphocyte and the immortality of the myeloma. ...
Soll is now the Director of the Developmental Studies Hybridoma Bank (DSHB), a NIH National Resource, Director of the W.M. Keck ... He is the current director of the Developmental Studies Hybridoma Bank, Monoclonal Antibody Research Institute, and the WM Keck ... Developmental Studies Hybridoma Bank WM. Keck Dynamic Image Analysis Facility. ...
CD4+CD25+ hybridomas have been generated from tolerant mice. These CD4+CD25+ hybridomas resemble in function the naturally ... CD25+ Inhibitory Hybridoma Clones. Field's research focuses on mechanisms of acquired immunologic tolerance by studying mouse ...
Adekar SP, Jones RM, Elias MD, Al-Saleem FH, Root MJ, Simpson LL, Dessain SK (2008) "Hybridoma populations enriched for ... "High efficiency creation of human monoclonal antibody-producing hybridomas." J Immunol Methods 291 (1-2): 109-122. doi:10.1016/ ... "High efficiency creation of human monoclonal antibody-producing hybridomas." J Immunol Methods 291 (1-2): 109-22. ...
... including hybridomas (BCCM/GeneCorner) • genetic material in a host or in the form of isolated material (e.g. plasmids, ...
This process is called Hybridoma technology. Laboratory animals (e.g., mice) are first exposed to an antigen against which we ...
"Activation-induced cytidine deaminase turns on somatic hypermutation in hybridomas". Nature. 415 (6873): 802-6. doi:10.1038/ ...
For production of monoclonal antibodies by producing hybridoma. For production of Induced stem cells. To assess protein ...
The hybridomas can be grown indefinitely in a suitable cell culture medium. They can also be injected into mice (in the ... The medium must be enriched during in vitro selection to further favour hybridoma growth. This can be achieved by the use of a ... Rabbit B-cells can be used to form a rabbit hybridoma. Polyethylene glycol is used to fuse adjacent plasma membranes, but the ... Only fused hybrid cells, referred to as hybridomas, are able to grow indefinitely in the media because the spleen cell partner ...
Shi, Y. F.; Sahai, B. M.; Green, D. R. (1989). "Cyclosporin a inhibits activation-induced cell death in T-cell hybridomas and ... "Activation-induced cell death in T cell hybridomas is due to apoptosis. Morphologic aspects and DNA fragmentation". Journal of ... "Role for c-myc in activation-induced apoptotic cell death in T cell hybridomas". Science. 257 (5067): 212-4. doi:10.1126/ ...
In 1973 he described, with Edward Cohen, a method of producing antibodies involving human-mouse hybrid cells, or hybridomas. ... In 1975, Georges Köhler, César Milstein, and Niels Kaj Jerne, succeeded in making hybridomas that made antibodies to known ... the beginnings of hybridoma technology". Journal of the History of Biology 25 (2): 175-230. doi:10.1007/BF00162840. PMID ...
who isolated IL17 transcript from a rodent T-cell hybridoma. The protein encoded by IL17A is a founding member of IL-17 family ...
At first, mouse hybridoma cells whose monoclonal antibodies target one of the desired antigens are produced. Independently, rat ... These two cell types are hybridised, yielding hybrid-hybridomas or quadromas, which produce hybrid (trifunctional) antibody as ... hybridoma cells targeting the other antigen are produced. ...
Protein tag Polyhistidine-tag Flag-tag Developmental Studies Hybridoma Bank 9E10. ... is available from the non-commercial Developmental Studies Hybridoma Bank. ...
The fused cells are called hybridomas, and will continually grow and secrete antibody in culture. Single hybridoma cells are ... most antibodies are produced by hybridoma cell lines through immortalization of antibody-producing cells by chemically-induced ...
... in fetal liver hybridomas. Proceedings of the National Academy of Sciences ...
Efetov was among the founders of the Hybridoma Laboratory at CSMU. Since 1986, he worked as the senior, chief, and then top ...
This review aims at discussing the reasons for hybridoma-derived idiotype vaccines, more difficult to produce but also more ... Most published trials based on hybridoma-derived idiotype vaccines are being analyzed, together with the yet non-peer reviewed ... All in all, the sole randomized trial ever conducted on hybridoma-derived idiotype vaccines failed to achieve its primary ... Hybridoma-derived idiotype vaccines have been used for the experimental treatment of human lymphoma over the last twenty years ...
A murine hybridoma with large cytoplasmic inclusions of kappa light chains.. D C Reason ... A murine hybridoma cell line has been established that consistently forms large cytoplasmic inclusions. These structures bind ...
Also disclosed are methods of simulating hybridoma cells to increase monoclonal antibody production by administering a CD40 ... Two of the hybridoma cell lines (designated hCD40L-M90 and hCD40L-M91) were selected for further expansion. These hybridoma ... A method for stimulating hybridoma cells to increase monoclonal antibody secretion, the method comprising contacting hybridoma ... A method for stimulating hybridoma cells to increase monoclonal antibody secretion, the method comprising contacting hybridoma ...
Hybridomas are screened for the required antibody; they are then cloned & produce that antibody. Magnification: x1500 at 35mm ... The fusion product is called a hybridoma cell. B lymphocytes are short-lived; when fused with tumour cells, which divide ... False-colour scanning electron micrograph of a hybridoma cell producing a monoclonal antibody to cytoskeleton protein. ... Hybridomas are screened for the required antibody; they are then cloned & produce that antibody. Magnification: x1500 at 35mm ...
CloneMedia for Mouse Hybridoma Generation provide a complete solution that supports all stages of hybridoma cell line ... Hybridoma Cell Line Development Workflow. Figure 1-1: XP Media and CloneMedia for Mouse Hybridoma Generation: Hybridoma Cell ... XP Media Hybridoma Fusion Medium. Fusion Medium. 500 mL. K8863. XP Media Hybridoma Fusion Recovery Medium. Recovery Medium. 100 ... XP Media Hybridoma Growth Medium (with HT). 500 mL. K8866. CloneMedia Hybridoma Semi-Solid Selection and Cloning Medium ( ...
Equivalence of human and mouse CD4 in enhancing antigen responses by a mouse class II-restricted T cell hybridoma. P von Hoegen ... Equivalence of human and mouse CD4 in enhancing antigen responses by a mouse class II-restricted T cell hybridoma.. J Exp Med 1 ... have examined the ability of hCD4 to interact functionally with mouse class II MHC molecules using the mouse T cell hybridoma ... demonstrate that expression of hCD4 results in an equivalent stimulation of antigen responses by this mouse T cell hybridoma. ...
Antibody & Hybridomas Production Services * Conjugates Production Activities * Hybridomas (Cell Lines) & Monoclonal Antibody ... Method : HYBRIDOMA TECHNOLOGY *Hybridoma Technology developed by Kohler and Milstein has been widely used for the production of ... Home Page Services Hybridomas (Cell Lines) & Monoclonal Antibody Production * Products * IVD Instruments * MicroELISA. * ... Hybridomas (Cell Lines) & Monoclonal Antibody Production. Adaltis can produce purified and not purified antibody by using its ...
METHODS OF PRODUCING HYBRIDOMAS AND MONOCLONAL ANTIBODIES AND ANTIBODIES PRODUCED THEREBY - diagram, schematic, and image 150. ... Back to METHODS OF PRODUCING HYBRIDOMAS AND MONOCLONAL ANTIBODIES AND ANTIBODIES PRODUCED THEREBY , All Patents . ...
Expression and function of the transfected CD8 alpha chain in murine T cell hybridomas ... CD4+ helper T cell hybridomas. However, hybridomas established by fusing a cytolytic T cell clone with BW5147 cell lines, ... Analysis of the antigen reactivity of the hybridomas revealed that the human CD8.alpha. chain failed to increase the mouse T ... chain molecules in mouse T cell hybridomas were analyzed. The expression of cytolytic T lymphocyte-derived CD8 molecules was ...
Point value for hybridomas, Word Decoder for hybridomas, Word generator using the letters hybridomas, Word Solver hybridomas, ... Possible Scrabble words with hybridomas, Anagram of hybridomas ... Definitions of hybridomas. Definition of "hybridomas" found in ... Definition of "hybridomas" from Dictionary.com Words that start with hybridomas Words that end with hybridomas Words that ... Scrabble point value for hybridomas: 21 points. Hybridomas is a Words with Friends word. Words with Friends point value for ...
... is an eagle-i resource of type Hybridoma cell line at eagle-i Network Shared Resource Repository. ... mouse anti-nucleoplasmin hybridoma (b7-1A9). eagle-i ID. http://shared.eagle-i.net/i/00000140-d7e2-ff6c-fa61-b3d480000000 ...
Some hybridomas in the ATCC collection are somatic cell hybrids. These cells are capable of producing immunoglobulins that are ... Hybridomas are immortalized cells derived from the fusion of B lymphoblasts with a myeloma fusion partner. ... Alphanumeric - Hybridomas Search a wide selection of hybridomas alphanumerically. Mouse Hybridomas Search for hybridomas by ... Hybridomas Hybridomas are immortalized cells derived from the fusion of B lymphoblasts with a myeloma fusion partner. Some ...
The hybridomas can be grown in culture, each culture starting with one viable hybridoma cell, producing cultures each of which ... Hybridomas at the US National Library of Medicine Medical Subject Headings (MeSH) "Hybridoma Technology". Understanding Cancer ... Once a hybridoma colony is established, it will continually grow in culture medium like RPMI-1640 (with antibiotics and fetal ... Hybridoma technology is a method for producing large numbers of identical antibodies (also called monoclonal antibodies). This ...
A rabbit hybridoma is a hybrid cell line formed by the fusion of an antibody producing rabbit B cell with a cancerous B-cell ( ... "Recombinant Human Interleukin-6 Enhances the Immunoglobulin Secretion of a Rabbit-Rabbit Hybridoma". Hybridoma. 20 (3): 189-98 ... The process of hybridoma formation in a rabbit first entails obtaining B-cells from a rabbit that has been immunized. There are ... Resulting antibodies from hybridomas are screened for an antigen which meets criteria of interest by diagnostic tests such as ...
... known as a hybridoma, multiplies rapidly in culture. Since the antibodies obtained from hybridomas are produced by clones ... hybrid cell, known as a hybridoma, multiplies rapidly in culture. Since the antibodies obtained from hybridomas are produced by ... As shown in step 4, only hybridomas can live in the HAT medium; unfused myeloma cells, lacking HGPRT, die in the medium, as do ... The resulting hybrid cells, called hybridomas, grow at the rate of myeloma cells but also produce large amounts of the desired ...
Hybridoma fusion and selection reagents are designed to assist fusion and select hybridomas from among normal cell populations ... Need Help Finding Hybridomas? View a comprehensive listing of ATCC hybridomas by antigenic determinant. ... Hybridoma Development * RPC5.4 (ATCC® TIB-12™) ATCC® Number: TIB-12™ Organism: Mus musculus, mouse ... PEG-4000 is used to assist in the fusion of myeloma and spleen cells to form hybridomas. ...
Hybridoma fusion and selection reagents are designed to assist fusion and select hybridomas from among normal cell populations ... Need Help Finding Hybridomas? View a comprehensive listing of ATCC hybridomas by antigenic determinant. ...
... hybridoma technology to the production of human monoclonal antibodies, human lymphoid lines producing antibody with defined... ... Weiss, R. A., 1982, Hybridomas produce viruses as well as antibodies, Immunol. Today 3: 292-294.CrossRefGoogle Scholar ... Kozbor, D., and Roder, J., 1984, In vitro stimulated lymphocytes as a source of human hybridomas, Eur. J. Immunol., 14: 23-27. ... Milstein, C., and Cuello, A. C., 1983, Hybrid hybridomas and their use in immunohistochemistry, Nature 305: 537-540.PubMed ...
... ,Perhaps sheer in-house demand is causing shortages in your laboratory or fellow researchers, ... Mouse Anti-Drosophila fasciclin III Monoclonal Antibody, Unconjugated from Developmental Studies Hybridoma Bank. 10. Hybridoma ... Hybridoma Development from QED Bioscience Inc.. 2. Mouse Anti-Xenopus laevis Xenopus nuclear factor Antibody, Unconjugated from ... Mouse Anti-Quail cell marker Antibody, Unconjugated from Developmental Studies Hybridoma Bank. 7. Mouse Anti-Human VCAM-1 ...
SECRETING HUMAN MYELOMA CELL LINE WILL BE DEVELOPED AND CHARACTERIZED FOR SPECIFIC APPLICATION TO HUMAN HYBRIDOMA PRO ... ...
The use of chitosan fibers as a matrix for immobilizing hybridoma cells was investigated. Optimal cell entrapment within ... Phillips, H.A., Scharer, J.M., Bols, N.C., Moo-Young, M. (1987). Effect of Oxygen on Antibody Production in Hybridoma Culture. ... Buehler, R.J. (1984). Production of monoclonal antibody from gel entrapped hybridoma cells.Karyon Technology News, 1, p 3. ... Chitosan fibers were found to be more effective than chitosan beads in the occlusion of the hybridoma cells. Maximum total cell ...
Bovine x mouse hybridomas that secrete bovine immunoglobulin G1 Message Subject. (Your Name) has forwarded a page to you from ... The interspecific fusion of normal bovine lymphocytes with a nonsecreting mouse hybridoma produced stable cell lines secreting ...
Hybridoma cell lines developed by Covance remain exclusive property of the client. A typical hybridoma development proje, ... Covance has generated hybridomas against a large variety of antigens. Prior to project initiation, you are provided with ... Hybridoma cell lines developed by Covance remain exclusive property of the client. A typical hybridoma development project has ... Mouse Anti-Xenopus laevis Xenopus nuclear factor Antibody, Unconjugated from Developmental Studies Hybridoma Bank. 3. Hybridoma ...
Opti-Clone II - Hybridoma Cloning Factor. Hybridoma Cloning Factor. MPs Opti-Clone™ is a partially purified hybridoma growth ... 50X Concentrate)Hypoxanthine 13.61 mg/LAminopterin 2H2O 0.1906 mg/LThymidine 3.876 mg/LSelective for hybridoma cell growth and ...
... chemically defined medium optimized for the growth of a variety of hybridomas and myelomas and the production of monoclonal ... CD Hybridoma Medium✝. CD Hybridoma AGT™✝✝. Hybridoma-SFM✝. PFHM-II. Media Type. Chemically-Defined Media. Chemically-Defined ... Hybridoma-SFM Serum-free formulation Hybridoma-SFM is a very low-protein medium (20 μg/mL) optimized for the growth of human, ... A-Gibco® CD Hybridoma Medium B-Gibco® IMDM+10%FBS C-Gibco® Hybridoma-SFM D, E and F are media from other manufacturers. ...
HCGS),br,,B,From Cultured Human Endothelial Cells,/B,,br,HCGS will significantly improve the hybridoma cell cloning efficiency ... Hybridoma cell growth supplement, 25 mL. CN¥0.00 Key features and details. Hybridoma Cell Growth Supplement (HCGS) from ... HCGS will significantly improve the hybridoma cell cloning efficiency while eliminating feeder cell need.. HCGS may be used in ... cultured human endothelial cells will significantly improve the hybridoma cell cloning efficiency while eliminating feeder cell ...
Early History of Specific Antibody-producing Lymphocyte Hybridomas Message Subject (Your Name) has forwarded a page to you from ...
After fusion and selection of 54 HAT-resistant hybridomas, three clones have been obtained (1F1, 2H4 and 4D7) that secrete ... Sample records for hybridomas. from WorldWideScience.org. Sample records 1 - 1 shown. ... hybridoma supernatants) and 1:20.000 (ascitis fluid). The three MAbs showed a wide spectrum of reactivity, recognizing antigens ...
Would anyone have any information on a hybridoma protocol which specifically targets IgM antibodies? I read that they appear ... IgM hybridoma protocol - posted in Immunology: Hello all, We are looking to generate an IgM antibody against our protein of ... Of MAJOR importance in obtaining a good number of hybridomas is the quality of your FCS. We always pre-screen batches by ... IgM hybridoma protocol. Started by TonyL, Jan 26 2009 04:21 PM ... Why do you would obtain an IgM hybridoma?? The IgG monoclonal ...
Screening hybridoma libraries for antibodies that bind to cell surface antigens is a time and resource intensive process. The ... Screening hybridoma libraries for antibodies that bind to cell surface antigens is a time and resource intensive process. The ... Application Note: Mix-and-Measure Assay for Screening Hybridoma Supernatants on IntelliCyt Platforms. 9 November 2018 ...
For instance, the T cell hybridoma that recognizes SIINFEKL/Kb - cant find the hybridoma for sale anywhere. ... I cant seem to find a vendor that sells T cell hybridomas that I know exist. ... Where do you purchase T cell hybridomas - posted in Immunology: ... Where do you purchase T cell hybridomas. Started by Ahrenhase, ... I cant seem to find a vendor that sells T cell hybridomas that I know exist. For instance, the T cell hybridoma that ...
Immunoregulatory lymphokines of T hybridomas from AIDS patients: constitutive and inducible suppressor factors ... Immunoregulatory lymphokines of T hybridomas from AIDS patients: constitutive and inducible suppressor factors ... Immunoregulatory lymphokines of T hybridomas from AIDS patients: constitutive and inducible suppressor factors ... Immunoregulatory lymphokines of T hybridomas from AIDS patients: constitutive and inducible suppressor factors ...
... Kang Liu kang.liu at worldnet.att.net Tue Sep 14 22:59:59 EST 1999 * ... I am trying to CD8 T cells from OT-I transgenic mouse (using T cell proliferation assay)and B3Z T-T hybridoma (measure IL-2 ... Next message: T cells from Transgenic TCR mouse v.s. T-T hybridoma ... Next message: T cells from Transgenic TCR mouse v.s. T-T hybridoma ...
Hybridoma,Production,Performance,medicine,medical news today,latest medical news,medical newsletters,current medical news, ... 1Pettit S, Santos M, Tanner T, Huang N. Enhanced Growth and Productivity of a Hybridoma with Recombinant Human Albumin and ... To obtain a copy of the above-mentioned article entitled, Enhanced Growth and Productivity of a Hybridoma with Recombinant ... This research supports what customers have been reporting from their use of Cellastim and Lacromin for hybridoma cell culture ...
BD Biosciences provides flow cytometers, reagents, tools, and a wide range of services to support the work of researchers and clinicians who understand disease and improve care ...
Hybridomas and monoclonal antibodies : presented at the Thirty-Fourth Annual Meeting of the American Association of Blood Banks ... schema:name "Hybridomas and monoclonal antibodies : presented at the Thirty-Fourth Annual Meeting of the American Association ... Hybridomas and monoclonal antibodies : presented at the Thirty-Fourth Annual Meeting of the American Association of Blood Banks ... Hybridomas and monoclonal antibodies : presented at the Thirty-Fourth Annual Meeting of the American Association of Blood Banks ...
Our Magic-HybridomaTM Platform allows generation of 50-200 mouse hybridoma clones per hybridoma cell fusion and relies on the ... Our Magic Hybridoma™ Platform allows generation of 50-200 mouse hybridoma clones per hybridoma cell fusion. This platform ... Magic Hybridoma™ Technology. Magic Hybridoma™ Technology. "Creative Biolabs is committed to providing highly customized ... Therefore, Magic Hybridoma™ Platform represents an revolutionary method to produce a large number of monoclonal antibodies for ...
... a human-murine hybridoma suitable as a fusion partner for immortalizing an antibody-secreting B cell has been generated. The ... The human-murine hybridoma (the "immortalizing hybridoma") is an immortal cell line which results from the fusion of a murine ... "Non-secreting" hybridoma refers to a hybridoma which is capable of continuous reproduction and, therefore, is immortal, which ... A hybridoma "having human characteristics" refers to a hybridoma which retains detectable human-derived chromosomes such as ...
Hybridoma cells were grown in clear polystyrene tissue culture plates. The hybridoma line, growth medium and the quantitation ... Hybridoma Master Cultures. Hybridoma master cultures were established in 96-well clear polystyrene tissue culture plates after ... Until recently, hybridoma-producing laboratories have pursued a labor-intensive and time-consuming serial path of hybridoma ... No Effect of CMFDA-labeling on Hybridoma Growth or Colony Formation. The repeated labeling of the hybridoma cells with CMFDA ...
... low energy ultrasonic resonance fields to transiently aggregate hybridoma cells and return them by sedimentation to a perfusion ... low energy ultrasonic resonance fields to transiently aggregate hybridoma cells and return them by sedimentation to a perfusion ...
  • Much of the work behind production of monoclonal antibodies is rooted in the production of hybridomas, which involves identifying antigen-specific plasma/plasmablast cells (ASPCs) that produce antibodies specific to an antigen of interest and fusing these cells with myeloma cells. (readtiger.com)
  • The stimulation of these hybridomas by fibroblast targets was not blocked by an anti-H-2k(k),D(k)-specific monoclonal antibody. (elsevier.com)
  • The effect of glucose and other monosaccharide availability in culture medium on production of antibody by human hybridomas has been studied. (elsevier.com)
  • Human hybridoma cells C5TN produce an anti lung cancer human monoclonal antibody, and the light chain is N-glycosylated at the variable region. (elsevier.com)
  • Ecotropic recombinant virus (ERV), a relatively new class of murine retrovirus endogenous to mice, is expressed at significant levels by most murine myeloma and hybridoma cells examined. (utmb.edu)
  • When fused with in vitro immunized human PBL, they generated several h x h x h x m hybridomas stably secreting high yields (10-23 micrograms/ml/24 h) of human mAbs reactive with recombinant HBV core antigen (rHBcAg). (unibs.it)
  • ERV isolated from murine hybridoma cells show a unique pattern of cross-reactivity with anti-MuLV env antibodies and this pattern is clearly distinct from that of ectropic and xenotropic retroviruses. (utmb.edu)
  • The expression of cytolytic T lymphocyte-derived CD8 molecules was suppressed in hybridomas established by fusing the BW5147 thymoma to a CD8+ cytolytic T lymphocyte clone, while expression of CD4 reamined intact in BW .times. (geoscience.net)
  • This is effected by fusing myeloma cell and mouse lymphocyte to form a hybrid cell ( hybridoma ). (readtiger.com)
  • These B cells were used as APC for antigen-specific T-cell hybridomas. (lu.se)
  • B cells from naïve mice did present native, triple-helical, CII (nCII) but also ovalbumin (OVA) and denatured CII (dCII) to antigen-specific T-cell hybridomas. (lu.se)
  • In addition, B cells primed with nCII or OVA, but not dCII, activated the antigen-specific T-cell hybridomas two to three times better than naïve B cells. (lu.se)
  • We now demonstrate that expression of hCD4 results in an equivalent stimulation of antigen responses by this mouse T cell hybridoma. (rupress.org)
  • These hybridomas could not be stimulated by spleen cells from H-2(i3), H-2(k), H-2 b or H-2(d) mice. (elsevier.com)
  • However, hybridomas established by fusing a cytolytic T cell clone with BW5147 cell lines, transfected with either the mCD8.alpha. (geoscience.net)
  • The media are optimized to support the selection and growth of hybridoma clones using Molecular Devices ClonePix™ line of mammalian colony pickers, but they are also compatible with other appropriate methods. (moleculardevices.com)
  • In 1975, Georges Köhler and César Milstein succeeded in making fusions of myeloma cell lines with B cells to create hybridomas that could produce antibodies, specific to known antigens and that were immortalized. (readtiger.com)
  • We have characterized two unusual T-cell hybridomas, 1E3 and 3B8, from H-2(k) mice immunized with I-A b -transfected L cells (H-2(k)), that are stimulated by L cells transfected with I-A b , I-A(k) or I-E b , but not by non-transfected L cells. (elsevier.com)
  • Lipopolysaccharide (LPS)-activated splenic and peritoneal exudate cells from H-2(k), H-2(d), H-2(i3), H-2 b as well as β 2 -microglobulin-deficient, TAP-1-deficient and I-Aα-deficient H-2 b mice stimulated these hybridomas. (elsevier.com)
  • The aim of this study was to establish hybridomas capable of long-term production of human monoclonal antibodies (mAbs). (unibs.it)
  • We have examined the ability of hCD4 to interact functionally with mouse class II MHC molecules using the mouse T cell hybridoma BI-141, specific for beef insulin. (rupress.org)
  • Equivalence of human and mouse CD4 in enhancing antigen responses by a mouse class II-restricted T cell hybridoma. (rupress.org)
  • Analysis of the antigen reactivity of the hybridomas revealed that the human CD8.alpha. (geoscience.net)
  • chain molecules in mouse T cell hybridomas were analyzed. (geoscience.net)
  • XP Media and CloneMedia for Mouse Hybridoma Generation provides a complete solution that supports all stages of hybridoma cell line development. (moleculardevices.com)
  • [ citation needed ] Rabbit B-cells can be used to form a rabbit hybridoma . (readtiger.com)
  • Hybridoma technology is a method for producing large numbers of identical antibodies (also called monoclonal antibodies). (wikipedia.org)
  • The hybridomas can be grown in culture, each culture starting with one viable hybridoma cell, producing cultures each of which consists of genetically identical hybridomas which produce one antibody per culture (monoclonal) rather than mixtures of different antibodies (polyclonal). (wikipedia.org)
  • In contrast to polyclonal antibodies, which are mixtures of many different antibody molecules, the monoclonal antibodies produced by each hybridoma line are all chemically identical. (wikipedia.org)
  • The next stage is a rapid primary screening process, which identifies and selects only those hybridomas that produce antibodies of appropriate specificity. (wikipedia.org)
  • Once a hybridoma colony is established, it will continually grow in culture medium like RPMI-1640 (with antibiotics and fetal bovine serum) and produce antibodies. (wikipedia.org)
  • Several efforts were made to generate rabbit monoclonal antibodies after the development of mouse hybridoma technology in the 1970s. (wikipedia.org)
  • Research was conducted into mouse-rabbit hetero-hybridomas to make rabbit monoclonal antibodies. (wikipedia.org)
  • Fusion of 240E-1 cells with rabbit lymphocytes produced hybridomas that secreted rabbit monoclonal antibodies in a consistent manner. (wikipedia.org)
  • Resulting antibodies from hybridomas are screened for an antigen which meets criteria of interest by diagnostic tests such as ELISA, Western blot, Immunohistochemistry and FACS. (wikipedia.org)
  • Since the antibodies obtained from hybridoma s are produced by clones derived from a single lymphocyte, they are called monoclonal antibodies. (britannica.com)
  • Several years before the application of Köhler and Milstein's (1975) hybridoma technology to the production of human monoclonal antibodies, human lymphoid lines producing antibody with defined antigenic specificity were established by Epstein-Barr virus (EBV) "immortalization" (Steinitz et al . (springer.com)
  • Gibco® CD Hybridoma Medium is a chemically defined, protein-free medium optimized for the production of monoclonal antibodies and recombinant proteins in human, mouse, and rat hybridomas and myelomas. (thermofisher.com)
  • PFHM-II is a protein-free medium optimized for the growth of human, mouse, and rat hybridomas and myelomas, and the production of monoclonal antibodies. (thermofisher.com)
  • After fusion and selection of 54 HAT-resistant hybridomas, three clones have been obtained (1F1, 2H4 and 4D7) that secrete IgG2a antibodies reactin (mais) g to BoHV-1 antigens. (worldwidescience.org)
  • Would anyone have any information on a hybridoma protocol which specifically targets IgM antibodies? (protocol-online.org)
  • The IgG monoclonal antibodies will have, always, more affinity, more specificity, and more antibody concentration than an IgM hybridoma secreting cell. (protocol-online.org)
  • Screening hybridoma libraries for antibodies that bind to cell surface antigens is a time and resource intensive process. (selectscience.net)
  • Therefore, Magic Hybridoma™ Platform represents an revolutionary method to produce a large number of monoclonal antibodies for each target. (creative-biolabs.com)
  • We frequently obtain over 50-200 hybridoma clones per fusion, thus providing the solid basis to isolate functional antibodies. (creative-biolabs.com)
  • New York, US-Mar 13, 2019 With abundant experience in recombinant antibody discovery and manufacturing, Creative Biolabs has been committed to the service about high-quality monoclonal antibodies by the hybridoma technology. (digitaljournal.com)
  • In general, the mAb is prepared by use of the hybridomas antibodies technology. (digitaljournal.com)
  • Based on the cell fusion technology, this technology allows for the fusion of sensitized B cells with the ability to secrete specific antibodies and myeloma cells capable of reproducing indefinitely, resulting in the production of B cell hybridoma. (digitaljournal.com)
  • On top of the hybridoma technology, Creative Biolabs also excels in the human hybridoma antibodies production using transgenic mice. (digitaljournal.com)
  • Besides, the transgenic technology also contributes to the discovery and development of hybridoma antibodies. (digitaljournal.com)
  • Hybridomas Producing Highly Specific Monoclonal Antibodies to Detect Mycobacterium Avium Subspecies Paratuberculosis" was invented by John P. Bannantine (Ames, Iowa). (highbeam.com)
  • S. Patent & Trademark Office: "Hybridoma cell lines which produce and secrete monoclonal antibodies which selectively bind to Mycobacterium avium subspecies paratuberculosis have been produced. (highbeam.com)
  • Splenocytes from mice bearing NNK-specific antibodies were used to create hybridomas. (mdpi.com)
  • Craig R. Halberstadt, Bernhard O. Palsson, A. Rees Midgley, and Rane L. Curl, "Optimization and Mathematical Modeling of the Transtubular Bioreactor for the Production of Monoclonal Antibodies from a Hybridoma Cell Line", Biotechnol. (umich.edu)
  • The resulting hybridoma cultures were screened for the production of antibodies directed against the soybean protoplasts and were then cloned. (eurekamag.com)
  • Hybridomas secreting antigen-specific antibodies are cloned to establish a stable line secreting a unique antibody with defined specificity and isotype. (wustl.edu)
  • gammadelta T cell hybridomas derived from mice infected with Listeria were preferentially stimulated by low density macrophages from peritoneal exudates of infected mice.Furthermore, primary splenic gammadelta T cells isolated from Listeria-infected mice were cytotoxic for low density macrophages in vitro, and cytotoxicity was inhibited in the presence of antibodies to the gammadelta TCR. (nih.gov)
  • Role of outer membrane protein H (OmpH)- and OmpA-specific monoclonal antibodies from hybridoma tumors in protection of mice against Pasteurella multocida. (asm.org)
  • The backpack hybridoma tumor system in BALB/c mice was used to continuously deliver immunoglobulin G2b (IgG2b) monoclonal antibodies (MAbs) specific for OmpH (MAb MT1) and OmpA (MAb MT4.1). (asm.org)
  • We studied the V region genes of three IgM hybridoma monoclonal antibodies (BUD 45.12.8, BUD 114.4.11 and BUD 94.91.8) which were selected for Sm reactivity and derived from B cells of a healthy child. (uwo.ca)
  • Production of hybridomas which secrete specific monoclonal antibodies need several steps to complete. (cainscrossing.org)
  • Screening of the antibodies: Hybridomas need to screened for desired antibody specificity. (cainscrossing.org)
  • Monoclonal antibodies are commonly assumed to be monospecific, but anecdotal studies have reported genetic diversity in antibody heavy chain and light chain genes found within individual hybridomas. (elsevier.com)
  • These findings, reflecting the current state of hybridomas used in research, reiterate the importance of using sequence-defined recombinant antibodies for research or diagnostic use. (elsevier.com)
  • Serum-free media are commonly used in the production of monoclonal antibodies in hybridoma and recombinant myeloma cells. (capricorn-scientific.com)
  • The Hybridoma Facility will produce monoclonal antibodies to antigens provided by investigators. (yu.edu)
  • In general Dr. Scharff and Ms. Buhl will meet with the Principle Investigator, or his or her representative, to discuss the scientific problem and make decisions about how to immunize animals, to screen for positive hybridomas once a fusion is done and on the types of antibodies that are required. (yu.edu)
  • 2 pages of Easy-to-do innovations and actions enshrouded in the pdf Human Hybridomas and Monoclonal Antibodies. (tsimicro.net)
  • Can spread and learn pdf Human Hybridomas and Monoclonal Antibodies carbohydrates of this g to Be data with them. (tsimicro.net)
  • Monoclonal Antibodies(MABs) Monoclonal Antibodies (MABs) MABs are antibodies that arise from a single clone of Hybridoma technology cells. (laislainy.com)
  • 13 Sep Hybridoma technology and application for monoclonal antibodies An antibody is a protein used by the immune system to identify and neutralize foreign objects like bacteria and viruses. (laislainy.com)
  • The Hybridoma Core will be used by 29 UM-RDCC investigators who are pursuing research projects that require monoclonal antibodies. (grantome.com)
  • The primary purpose of the Hybridoma Core is to generate somatic-cell hybrids (hybridomas) that produce monoclonal antibodies of desired specificity. (grantome.com)
  • Since its establishment in 1980 the Hybridoma Facility has produced monoclonal antibodies against a wide variety of lymphocyte surface antigens, tumor cell antigens, purified proteins, cytokines, hormones, hormone receptors and recombinant proteins. (grantome.com)
  • Through these initiatives, the Hybridoma Core will remain on the cutting edge of monoclonal antibody technology, and continue to provide optimal service to a broad range of users who will contribute to the public health by using monoclonal antibodies for studying the pathogenesis, diagnosis and treatment of rheumatic diseases. (grantome.com)
  • HybridomaPlus provides the easiest and safest solution in the production of diverse antibodies with recombinant myeloma lines as well as traditional hybridoma. (capricorn-scientific.com)
  • A simple hybridoma screening method for high-affinity monoclonal antibodies using the signal ratio obtained from time-resolved fluorescence assay. (semanticscholar.org)
  • In order to preserve and stably produce excellent antibodies for long time, antibody genes in hybridoma cells can be extracted and sequenced. (sinobiological.com)
  • These isolated B cells are in turn fused with immortal B cell cancer cells, a myeloma, to produce a hybrid cell line called a hybridoma, which has both the antibody-producing ability of the B-cell and the exaggerated longevity and reproductivity of the myeloma. (wikipedia.org)
  • This maintains the well-being of the hybridomas and provides enough cells for cryopreservation and supernatant for subsequent investigations. (wikipedia.org)
  • The process of hybridoma formation in a rabbit first entails obtaining B-cells from a rabbit that has been immunized. (wikipedia.org)
  • After immunization, B-cells are fused with a candidate rabbit fusion partner cell line to form hybridomas. (wikipedia.org)
  • Hybridomas are immortalized cells derived from the fusion of B lymphoblasts with a myeloma fusion partner. (atcc.org)
  • The resulting hybrid cells, called hybridoma s, combine the longevity of the myeloma cell with the ability to produce a specific antibody and so are able to produce potentially unlimited amounts of the desired antibody. (britannica.com)
  • PEG-4000 is used to assist in the fusion of myeloma and spleen cells to form hybridomas. (atcc.org)
  • The use of chitosan fibers as a matrix for immobilizing hybridoma cells was investigated. (springer.com)
  • Chitosan fibers were found to be more effective than chitosan beads in the occlusion of the hybridoma cells. (springer.com)
  • Gibco® CD Hybridoma Medium results in higher viable cell densities and antibody yield in Hybridoma TP4-3.1 cells than other media. (thermofisher.com)
  • In comparative cell growth studies, Hybridoma TP4-3.1 (proprietary) cells growing in IMDM + 10% FBS were adapted to growth in other media through a minimum of 3 passages prior to evaluation of growth and monoclonal antibody production in batch culture. (thermofisher.com)
  • Hybridoma Cell Growth Supplement (HCGS) from cultured human endothelial cells will significantly improve the hybridoma cell cloning efficiency while eliminating feeder cell need. (mpbio.com)
  • I am trying to CD8 T cells from OT-I transgenic mouse (using T cell proliferation assay)and B3Z T-T hybridoma (measure IL-2 activation), both of which recognize the same epitope SIINFEKL on Kb molecules. (bio.net)
  • Article in Spring edition of Bioprocessing Journal reports InVitria's recombinant human serum albumin (Cellastim) and recombinant lactoferrin (Lacromin) cell culture supplements optimize cell growth and productivity in hybridoma cells. (bio-medicine.org)
  • Fort Collins, CO (PRWEB) April 22, 2009-- Recent studies using both classical media and the leading commercial serum-free hybridoma media formulations proved that InVitria's cell culture supplements optimized cell growth and productivity of hybridoma cells.1 Further, the studies revealed that Cellastim supports cell adaptation and survival in chemically-defined media. (bio-medicine.org)
  • Hybridoma cells were grown in clear polystyrene tissue culture plates. (moleculardevices.com)
  • Acoustic cell filter for high density perfusion culture of hybridoma cells. (biomedsearch.com)
  • We have developed a flow-through device which uses high frequency, low energy ultrasonic resonance fields to transiently aggregate hybridoma cells and return them by sedimentation to a perfusion bioreactor. (biomedsearch.com)
  • Also disclosed are methods of simulating hybridoma cells to increase monoclonal antibody production by administering a CD40 ligand polypeptide that stimulates B cell proliferation. (google.com.au)
  • A protein-free medium for the growth of hybridomas and other cells of the immune system. (nih.gov)
  • ABC supports the long-term growth of murine hybridomas as well as other transformed cells of the immune system. (nih.gov)
  • Hybridoma cells are inoculated into certain animals to produce ascites. (digitaljournal.com)
  • Within 3 weeks, the reactivity of hybridomas to histoplasmin declined dramatically, yet the cells responded vigorously to yeast-phase preparations that were enriched for cytosol, cell wall, or cell membrane. (asm.org)
  • Of 134 T cell hybridomas obtained from the fusion of C3H/HeJm or B10.HTT T cells stimulated with C57BL/6 splenocytes, nine T cell hybridomas were reactive to class I antigens and 126 T cell hybridomas were reactive to class II antigens. (jimmunol.org)
  • Expression in HEK 293 cells revealed a functional F(ab) with similar binding features to that of the parent hybridoma. (mdpi.com)
  • In this work, the interaction between a rat cortical thymic epithelial cell (TEC) line (R-TNC.1) with nursing activity and thymocytes as well as BWRT 8 thymocyte hybridoma (TH) cells has been studied. (hindawi.com)
  • Hybridoma - are cells that have been engineered to produce a desired antibody in large amounts. (academic.ru)
  • The hybridoma is immortal in the laboratory and makes the same products as its parent cells forever. (academic.ru)
  • To improve patient immune recognition of autologous tumor cells, we have developed a tumor B-cell lymphocyte hybridoma (TBH) autovaccination protocol. (nih.gov)
  • This approach is based on immunization of a cancer patient with a hybridoma cell suspension derived from the fusion of autologous activated B-cells and autologous cancer cells. (nih.gov)
  • Recent isolates of RX54-3 hybridoma cells (new cells) protect BALB/c mice against subsequent challenge with the tumorigenic myeloma parent cells used to construct this hybridoma. (aacrjournals.org)
  • In contrast, hybridoma cells which have been maintained in tissue culture for long periods of time (old cells) are not protective. (aacrjournals.org)
  • In the present study, we compared a number of properties of the new and old hybridoma cells and determined which line was more similar to the parent myeloma. (aacrjournals.org)
  • Therefore, in order to protect against challenge with the tumorigenic myeloma parent, hybridoma cells must retain properties of that parent. (aacrjournals.org)
  • Some component in the rat hybridoma supernatant influenced gamma retrovirus infection, either with the cellular Pit receptor or with the envelope proteins, when added to human TE cells and gamma retrovirus. (biology-online.org)
  • If you already have experience with hybridoma selection in liquid HAT medium, plate the same number of cells per well in the semi-solid medium as you would in liquid medium. (stemcell.com)
  • Comparison of the average cell numbers does not demonstrate consistently higher accumulation of T-cell hybridomas in the paws with higher inflammation scores, and injecting more cells does not cause increased accumulation. (spiedigitallibrary.org)
  • The traditional use of feeder cells to support the outgrowth of newly-created hybridomas has many disadvantages. (qedbio.com)
  • Batch-to-batch variations may occur in the preparations of feeder cells, feeder cells are a potential source of contamination, and feeder cells may compete for vital nutrients with freshly-fused hybridomas. (qedbio.com)
  • Each batch of BriClone is tested for its ability to support the outgrowth of hybridomas from freshly fused cells and for its ability to support the growth of hybridomas under conditions of limiting dilution. (qedbio.com)
  • In this study, we examined whether SK2 hybridoma cells microencapsulated in an alginate-poly(L)lysine-alginate (APA) membrane (APA-SK2 cells) were immunoisolated from the allogeneic host's immune system using a cytotoxicity test. (nii.ac.jp)
  • Immunological studies of SK2 hybridoma cells microencapsulated with alginatepoly(L)lysine-alginate(APA)membrane following allogeneic transplantation. (nii.ac.jp)
  • Cytomedical therapy for IgG1 plasmacytosis in human interleukin-6 transgenic mice using hybridoma cells microencapsulated in alginate-poly(L)lysine-alginate membrane. (nii.ac.jp)
  • Publications] Okada N., Miyamoto H., Yoshioka T., Sakamoto K., Katsume A., Saito H., Nakagawa S., Ohsugi Y., Mayumi T.: 'Immunological studies of SK2 hybridoma cells microencapsulated with alginate-poly (L) lysine-alginate (APA) membrane following allogeneic transplantation' Biochem.Biophys.Res.Commun.230. (nii.ac.jp)
  • Immunized spleen cells derived from either mice or Armenian hamsters are fused and maintained at the Hybridoma Center. (wustl.edu)
  • On chip real time monitoring of B-cells hybridoma secretion of immunoglobulin. (inserm.fr)
  • Light scatter properties and synthesis of IFN-γ by γδ T cell hybridomas was determined by electronically gating on CFSE+ cells. (nih.gov)
  • B) IFN-γ-producing hybridoma cells exhibit blast-like morphology. (nih.gov)
  • However, an enhanced phagocytosis by polymorphonuclear cells (PMNs) was observed in mice bearing MT1 hybridoma tumors. (asm.org)
  • Because of variable loss of chromosomes, cloned hybridoma cells from one fusion exhibit different fingerprint patterns. (iospress.com)
  • Further evidence for this hypothesis was provided by experiments in which H-2K(b) produced in Drosophila melanogaster cells and immobilized on the surface of a tissue culture plate was able to stimulate hybridomas derived from these alloreactive T cells. (nih.gov)
  • A) Reactivity of alloreactive anti H-2Kb hybridoma 34.9 with non-transfected T2 and T2 cells transfected with H-2Kb. (nih.gov)
  • To assay the ability of the anti-H-2Kb alloreactive T cells to recognize the H-2Kb produced by Drosophila melanogaster cells, T cell hybridomas were produced. (nih.gov)
  • Hybridomas were prepared from clones 34 and 59, and both reacted readily with T2 cells transfected with H-2Kb (data for hybridoma 34.9, derived from clone no. 34, shown in Fig. 9 A) and not with T2 cells transfected with the H-2Ld molecule (data not shown). (nih.gov)
  • The fundamental of hybridoma technology is based on the immortalization of B lymphocyte cells having antibody producing capacity but limited growth characteristics in vitro. (cainscrossing.org)
  • When incubation is finished, the viable cells are our hybridomas and these cells are again transferred to the normal culture medium. (cainscrossing.org)
  • HybridomaPlus is a completely defined, protein-free medium for the cultivation of various hybridoma cells. (capricorn-scientific.com)
  • Hybridoma Supplement is a completely defined growth supplement for fusion, selection and cloning of hybridoma cells. (capricorn-scientific.com)
  • Since Hybridoma Supplement must simply be added to the culture medium, there is no need for the more difficult use of feeder cells. (capricorn-scientific.com)
  • Hybrid cells propagate by employing Hybridoma technology that may be cultured in a laboratory or subcultured in a mouse bodily cavity. (espritrock.org)
  • These hybrid cells produced monoclonal antibody and this process is point out as Hybridoma technology. (espritrock.org)
  • the resulting hybridoma cells will produce the same secretion as the normal parent cells and proliferate indefinitely in culture like the parent tumor cells. (academic.ru)
  • hybridoma - A cell hybrid in which a tumour cell forms one of the original source cells. (academic.ru)
  • We therefore conclude that CsA and FK506 block activation-induced apoptosis in T cell hybridomas predominantly by interfering with activation signals leading to FasL expression and, further, that the regulation of the expression of Fas and FasL on activated T cells is differentially controlled. (uni-konstanz.de)
  • PowerPoint Presentation: Hybridomas are cells which are have been engineered to generate a. (laislainy.com)
  • primary hybridoma cells in selective-media, specifically media containing 1x concentration HAT for popularly known as hybridoma technology.1 Hybridoma. (laislainy.com)
  • Our Hybridoma Supplement replaces the formerly used feeder cells to eliminate disadvantages like overgrowth of newly formed hybridoma, source of contamination, competition for nutrients, and variations in growth factor concentrations. (capricorn-scientific.com)
  • During preservation, hybridoma cells may lost antibody genes, or difficult to be recovered, or even dead, which will affect antibody production . (sinobiological.com)
  • A typical hybridoma development project has four phases: immunization, fusion, cloning and hybridoma stabilization. (bio-medicine.org)
  • HCGS will significantly improve the hybridoma cell cloning efficiency while eliminating feeder cell need. (mpbio.com)
  • Until recently, hybridoma-producing laboratories have pursued a labor-intensive and time-consuming serial path of hybridoma culturing and limiting dilution cloning to achieve clonality. (moleculardevices.com)
  • How is Hybridoma Cloning Factor abbreviated? (thefreedictionary.com)
  • The methylcellulose-based semi-solid Medium D contains the selective reagents hypoxanthine, aminopterin and thymidine (HAT), therefore combining HAT selection and cloning of hybridomas into a single step. (stemcell.com)
  • This method enables isolation of producing hybridomas with a high probability of monoclonality after only a single round of cloning. (stemcell.com)
  • On the day of fusion, place the hybridoma semi-solid cloning medium, ClonaCell™-HY Medium D (Catalog #03804), at 2 - 8°C to thaw overnight. (stemcell.com)
  • BriClone hybridoma cloning medium has been developed to overcome and eliminate these common problems. (qedbio.com)
  • Add BriClone Hybridoma Cloning Medium to hybridoma culture medium as a 5% (v/v) supplement. (qedbio.com)
  • BriClone is suitable for use in the post-fusion stages of hybridoma production and when cloning hybridoma cell lines under conditions of limiting dilution. (qedbio.com)
  • The hybridomas analyzed were not biased towards those with cloning difficulties or known to have additional chains. (elsevier.com)
  • The interspecific fusion of normal bovine lymphocytes with a nonsecreting mouse hybridoma produced stable cell lines secreting bovine immunoglobulins. (sciencemag.org)
  • Stabilizing antibody secretion of human Epstein Barr virus-activated B-lymphocytes with hybridoma formation by electrofusion. (nih.gov)
  • These MAbs reacted with viral antigens in immunofluorescence (IFA) and immunoperoxidase (IPX) in dilutions up to 1:640 (hybridoma supernatants) and 1:20.000 (ascitis fluid). (worldwidescience.org)
  • Thanks to our advanced hybridoma technology , our company has the ability to provide the one-stop custom production of mAbs. (digitaljournal.com)
  • MAbs were detected in serum and peritoneal lavage samples of mice bearing hybridoma tumors by an enzyme-linked immunosorbent assay and an immunoblot assay. (asm.org)
  • Nevertheless, the potential of mAbs derived specifically from technology based on human hybridomas remains largely unfulfilled. (asmscience.org)
  • The principal reason for the lack of a large number of hybridoma-derived mAb therapeutics has simply been the technical difficulty in generating stable hybridomas that secrete human mAbs of high affinity and functional activity. (asmscience.org)
  • This chapter reviews recent efforts to develop and employ novel methods for the efficient generation of human hybridomas secreting human mAbs for clinical use. (asmscience.org)
  • We have previously reported that activation of murine T cell hybridomas leads to expression of Fas (CD95) and its ligand (FasL) which subsequently interact, even on the same cell, leading to apoptotic cell death. (uni-konstanz.de)
  • Serum-Free/Protein Free Hybridoma Medium is derived from F-12 and contains 10 µM of copper. (sigmaaldrich.com)
  • By careful screening and mutation, a human-murine hybridoma suitable as a fusion partner for immortalizing an antibody-secreting B cell has been generated. (freepatentsonline.com)
  • Hybridoma technology includes cell fusion, the plating of master cell cultures, and antibody screening. (moleculardevices.com)
  • Third, hybridoma generation and screening. (digitaljournal.com)
  • Syngene also offers services surrounding Hybridoma generation and screening. (syngeneintl.com)
  • After the fusion is performed, the Hybridoma Center will notify the investigator when to expect supernatants for screening. (wustl.edu)
  • ELISA screening may also be contracted to the Hybridoma Center. (wustl.edu)
  • In cases where the screen for positive hybridomas requires special expertise, someone from the PI's lab participates in the initial screening. (yu.edu)
  • In hybridoma screening, quantitative kinetic evaluation is difficult since the concentration of each antibody in the hybridoma supernatant is unknown. (semanticscholar.org)
  • Upon engagement of the TCR, hybridomas prepared using this fusion partner produce β-galactosidase which can be detected by a colormatic assay. (nih.gov)
  • B cell hybridomas were cultured on the chip and the secretion of immunoglobulins (antibody) specific to HEL was monitored in real-time and detected within only few minutes rather than after a 30-60 min incubation with standard ELISA experiments. (inserm.fr)
  • When such a single hybridoma cell forms a cell population, a specific antibody against an antigen epitope, also known as the mAb, can be prepared. (digitaljournal.com)
  • A balanced supplementation method was applied to develop a serum and protein- free medium supporting hybridoma cell batch culture. (epfl.ch)
  • Effect of Oxygen on Antibody Production in Hybridoma Culture. (springer.com)
  • InVitria Reveals Technology to Optimize Hybridoma Production P... ( Article in S. (bio-medicine.org)
  • Its service portfolio includes mouse and rat monoclonal antibody production using hybridoma technology, human, monkey, rabbit, chicken, dog, llama and camel monoclonal antibody production using various antibody library technologies. (digitaljournal.com)
  • In this report we describe a functional role for Shc in two events that occur during T cell activation, AICD and IL-2 production, in a T cell hybridoma line that has been previously shown to undergo apoptosis and produce IL-2 upon cross-linking of the TCR/CD3 complex. (jimmunol.org)
  • But since 2009, there forces reached an pdf Human Hybridomas of profound components that can edit 80 request field for two subareas at service production( 70F). (tsimicro.net)
  • 24 Feb Monoclonal antibody production using Hybridoma Technology. (laislainy.com)
  • One-stop service from antigen production - antibody development - hybridoma antibody gene sequencing - chimeric antibody production to humanized antibody development etc. (sinobiological.com)
  • Functional analysis of Histoplasma capsulatum-reactive T-cell hybridomas. (asm.org)
  • The role of class II MHC molecules in the activation of class I-reactive T cell hybridomas. (jimmunol.org)
  • To examine the role of Ia molecules in T cell responses to allo-class I major histocompatibility antigens, a series of allo-class I-reactive T cell hybridomas was established. (jimmunol.org)
  • XP Media and CloneMedia for Mouse Hybridoma Generation provides a complete solution that supports all stages of hybridoma cell line development. (moleculardevices.com)
  • Search a wide selection of hybridomas alphanumerically. (atcc.org)
  • Selected subclones were further tested for their ability to produce a stable hybridoma and monoclonal antibody secretion. (wikipedia.org)
  • Since the immunosuppressive drugs cyclosporin A (CsA) and FK506 block activation-induced apoptosis in T cell hybridomas, we examined whether such compounds affect cell death by interfering with expression of Fas, FasL or both, or whether they block Fas signal transduction. (uni-konstanz.de)
  • Some hybridomas in the ATCC collection are somatic cell hybrids. (atcc.org)
  • A) Reactivity of alloreactive anti H-2Kb hybridoma 34. (nih.gov)
  • B) Reactivity of hybridoma 34.9 to immobilized soluble H-2Kb treated with either PBS at pH 7.4 (middle) or 100 μM sodium acetate/acetic acid buffer at pH 3.0 (right). (nih.gov)
  • The hybridoma culture supernatant, secondary enzyme labeled conjugate, and chromogenic substrate, are then incubated, and the formation of a colored product indicates a positive hybridoma. (wikipedia.org)
  • False-colour scanning electron micrograph of a hybridoma cell producing a monoclonal antibody to cytoskeleton protein. (sciencephoto.com)
  • This Application Note addresses the need to verify hybridoma cell clonality in an automated fashion. (moleculardevices.com)
  • Hi, I had a strange effect of rat hybridoma supernatant that is not an effect of the MAB itself ( 83A25). (biology-online.org)
  • The Washington University School of Medicine Hybridoma Center is under the faculty supervision of Kathleen Sheehan, PhD and is operated by Research Technicians Rebecca Andrews and Chelsea Mannie. (wustl.edu)
  • This monoclonal antibody is produced by using a technology called "Hybridoma technology" which was discovered by George Kohler of West Germany and Cesal Milstein of Argentina in 1975 (Global-research-online.net, 2018). (cainscrossing.org)
  • 2018). The Immune System Hybridoma Technology Biochem t. online Available at: http://imgdealix.pw/The-Immune-System-Hybridoma-Technology-Biochem-t.html Accessed 4 Nov. 2018. (cainscrossing.org)
  • CD Hybridoma Medium is available in a ready-to-use (1X) liquid format and in our easy-to-use Advanced Granulation Technology™ (AGT™) format. (thermofisher.com)
  • The Washington University School of Medicine Hybridoma Center (WUHC) provides monoclonal antibody technology to the University research community. (wustl.edu)
  • In the course of making the hybridomas, students and fellows can receive training on how to carry out the hybridoma technology. (yu.edu)
  • 12 Mar Explore Hybridoma Technology with Free Download of Seminar Report and PPT in PDF and DOC Format. (laislainy.com)
  • The current proposal will allow this facility to continue to provide up-to-date hybridoma technology for UM-RDCC laboratories. (grantome.com)
  • Why Use Hybridoma Qualified Fetal Bovine Serum? (rndsystems.com)
  • Be the first to review Fetal Bovine Serum - Hybridoma Qualified and earn rewards! (rndsystems.com)
  • Have you used Fetal Bovine Serum - Hybridoma Qualified? (rndsystems.com)
  • Certain media such as Iscove's Modified Dulbecco's Medium (IMDM) which is often used as a base for development of serum-free hybridoma media do not contain copper in their basal formulations. (sigmaaldrich.com)
  • 50 ml Serum-free Hybridoma Supplement (10x) Cat. (capricorn-scientific.com)
  • Serum-free cultured hybridoma. (riken.jp)
  • The hybridoma line, growth medium and the quantitation of human IgG were provided by Medarex (Milpitas, CA). The ImageXpress Velos System was configured with a 488 nm laser. (moleculardevices.com)
  • The requirement of hybridoma growth for transferrin has been met by substituting the soluble organo-iron compound, sodium nitroprusside. (nih.gov)
  • Substantial improvement in the growth of hybridomas was afforded by the inclusion of 18 trace elements complexed to disodium ethylene diaminetetraacetate (EDTA). (nih.gov)
  • Hybridoma Qualified lots must show superior growth promotion, exceptional clonal growth at limiting dilution, and a lack of cytotoxicity during the growth studies. (rndsystems.com)
  • The transfected clone showed a 50-fold increased sensitivity towards BI in comparison to the same CD4- hybridoma. (rupress.org)
  • Conventional methods to select and clone antibody-producing hybridomas involve multiple dilution steps in liquid medium. (stemcell.com)
  • Search for hybridomas by mouse, lymphoblast or somatic cell used to develop the line. (atcc.org)
  • We have transfected the mouse CD4 gene into a beef insulin (BI)-specific murine T helper hybridoma that lacks CD4 surface expression. (rupress.org)
  • Analysis of the interaction between an alpha (1----6)dextran-specific mouse hybridoma antibody and dextran B512 by affinity electrophoresis. (biomedsearch.com)
  • The mouse or Armenian hamster host to be fused may be obtained, immunized, boosted and bled by the investigator or can be contracted through the Hybridoma Center. (wustl.edu)
  • Equivalence of human and mouse CD4 in enhancing antigen responses by a mouse class II-restricted T cell hybridoma. (rupress.org)
  • We have examined the ability of hCD4 to interact functionally with mouse class II MHC molecules using the mouse T cell hybridoma BI-141, specific for beef insulin. (rupress.org)
  • We now demonstrate that expression of hCD4 results in an equivalent stimulation of antigen responses by this mouse T cell hybridoma. (rupress.org)
  • A hybridoma is a cell line in which an antibody- producing B cell (human) is fused to an immortalized or long-lived mouse cell to produce. (laislainy.com)
  • Multiwell plates are used initially to grow the hybridomas, and after selection, are changed to larger tissue culture flasks. (wikipedia.org)
  • Hybridoma cell lines EP-16 and 7P41A were cloned by limiting dilution under conditions designed to seed 1 cell/well in 96-well plates. (qedbio.com)
  • Shc is rapidly phosphorylated on tyrosine residues in response to TCR engagement, and phosphorylated Shc subsequently binds to Grb2 and mSos, two proteins involved in Ras activation, in both T cell hybridomas and normal human PBL ( 4 ). (jimmunol.org)
  • A murine hybridoma cell line has been established that consistently forms large cytoplasmic inclusions. (rupress.org)
  • Three of these T cell hybridomas, HTB-157.7, HTB-176.10, and HTB-177.2, could react to the EL-4 cell line that expresses H-2Kb and H-2Db class I antigens but lacks class II I-Ab molecules. (jimmunol.org)
  • In contrast, the other three T cell hybridomas, CB-127.6, CB-221.7, and HTB-102.7, failed to react with EL-4 but reacted with the LB cell line which expresses class I (H-2Kb, H-2Db) and class II (I-Ab) molecules. (jimmunol.org)
  • Hybridoma cell line 500 expresses monoclonal antibody specific for Moloney murine leukemia virus (MuLV) gp80. (nih.gov)
  • Because it is manufactured with animal origin-free materials, protein purification and downstream processing of cultures grown in CD Hybridoma Medium is streamlined compared to that required for cultures grown in less defined media. (thermofisher.com)
  • The hybridoma antibody gene sequencing service provided by Sino Biological not only can quickly obtain the mAb gene, but also can use its advanced codon optimization and gene synthesis strategies to engineer the expression and purification of the antibody. (sinobiological.com)
  • Furthermore, the activation of these three T cell hybridomas with C57BL/6-derived splenocytes was not blocked by either anti-I-A or anti-L3T4 antibody. (jimmunol.org)