Hybridomas. Medical search

Cells artificially created by fusion of activated lymphocytes with neoplastic cells. The resulting hybrid cells are cloned and produce pure MONOCLONAL ANTIBODIES or T-cell products, identical to those produced by the immunologically competent parent cell.

Antibodies produced by a single clone of cells.

The property of antibodies which enables them to react with some ANTIGENIC DETERMINANTS and not with others. Specificity is dependent on chemical composition, physical forces, and molecular structure at the binding site.

Sites on an antigen that interact with specific antibodies.

Lymphocytes responsible for cell-mediated immunity. Two types have been identified - cytotoxic (T-LYMPHOCYTES, CYTOTOXIC) and helper T-lymphocytes (T-LYMPHOCYTES, HELPER-INDUCER). They are formed when lymphocytes circulate through the THYMUS GLAND and differentiate to thymocytes. When exposed to an antigen, they divide rapidly and produce large numbers of new T cells sensitized to that antigen.

That region of the immunoglobulin molecule that varies in its amino acid sequence and composition, and comprises the binding site for a specific antigen. It is located at the N-terminus of the Fab fragment of the immunoglobulin. It includes hypervariable regions (COMPLEMENTARITY DETERMINING REGIONS) and framework regions.

The largest of polypeptide chains comprising immunoglobulins. They contain 450 to 600 amino acid residues per chain, and have molecular weights of 51-72 kDa.

Lymphoid cells concerned with humoral immunity. They are short-lived cells resembling bursa-derived lymphocytes of birds in their production of immunoglobulin upon appropriate stimulation.

Large, transmembrane, non-covalently linked glycoproteins (alpha and beta). Both chains can be polymorphic although there is more structural variation in the beta chains. The class II antigens in humans are called HLA-D ANTIGENS and are coded by a gene on chromosome 6. In mice, two genes named IA and IE on chromosome 17 code for the H-2 antigens. The antigens are found on B-lymphocytes, macrophages, epidermal cells, and sperm and are thought to mediate the competence of and cellular cooperation in the immune response. The term IA antigens used to refer only to the proteins encoded by the IA genes in the mouse, but is now used as a generic term for any class II histocompatibility antigen.

Any discrete, presumably solitary, mass of neoplastic PLASMA CELLS either in BONE MARROW or various extramedullary sites.

Fusion of somatic cells in vitro or in vivo, which results in somatic cell hybridization.

Any cell, other than a ZYGOTE, that contains elements (such as NUCLEI and CYTOPLASM) from two or more different cells, usually produced by artificial CELL FUSION.

Local surface sites on antibodies which react with antigen determinant sites on antigens (EPITOPES.) They are formed from parts of the variable regions of FAB FRAGMENTS.

Serological reactions in which an antiserum against one antigen reacts with a non-identical but closely related antigen.

Polypeptide chains, consisting of 211 to 217 amino acid residues and having a molecular weight of approximately 22 kDa. There are two major types of light chains, kappa and lambda. Two Ig light chains and two Ig heavy chains (IMMUNOGLOBULIN HEAVY CHAINS) make one immunoglobulin molecule.

Unique genetically-controlled determinants present on ANTIBODIES whose specificity is limited to a single group of proteins (e.g., another antibody molecule or an individual myeloma protein). The idiotype appears to represent the antigenicity of the antigen-binding site of the antibody and to be genetically codetermined with it. The idiotypic determinants have been precisely located to the IMMUNOGLOBULIN VARIABLE REGION of both immunoglobin polypeptide chains.

Genes encoding the different subunits of the IMMUNOGLOBULINS, for example the IMMUNOGLOBULIN LIGHT CHAIN GENES and the IMMUNOGLOBULIN HEAVY CHAIN GENES. The heavy and light immunoglobulin genes are present as gene segments in the germline cells. The completed genes are created when the segments are shuffled and assembled (B-LYMPHOCYTE GENE REARRANGEMENT) during B-LYMPHOCYTE maturation. The gene segments of the human light and heavy chain germline genes are symbolized V (variable), J (joining) and C (constant). The heavy chain germline genes have an additional segment D (diversity).

One of the types of light chains of the immunoglobulins with a molecular weight of approximately 22 kDa.

Morphologic alteration of small B LYMPHOCYTES or T LYMPHOCYTES in culture into large blast-like cells able to synthesize DNA and RNA and to divide mitotically. It is induced by INTERLEUKINS; MITOGENS such as PHYTOHEMAGGLUTININS, and by specific ANTIGENS. It may also occur in vivo as in GRAFT REJECTION.

Molecules on the surface of T-lymphocytes that recognize and combine with antigens. The receptors are non-covalently associated with a complex of several polypeptides collectively called CD3 antigens (ANTIGENS, CD3). Recognition of foreign antigen and the major histocompatibility complex is accomplished by a single heterodimeric antigen-receptor structure, composed of either alpha-beta (RECEPTORS, ANTIGEN, T-CELL, ALPHA-BETA) or gamma-delta (RECEPTORS, ANTIGEN, T-CELL, GAMMA-DELTA) chains.

A group of genetically identical cells all descended from a single common ancestral cell by mitosis in eukaryotes or by binary fission in prokaryotes. Clone cells also include populations of recombinant DNA molecules all carrying the same inserted sequence. (From King & Stansfield, Dictionary of Genetics, 4th ed)

Antigens on surfaces of cells, including infectious or foreign cells or viruses. They are usually protein-containing groups on cell membranes or walls and may be isolated.

Proteins, protein complexes, or glycoproteins secreted by suppressor T-cells that inhibit either subsequent T-cells, B-cells, or other immunologic phenomena. Some of these factors have both histocompatibility (I-J) and antigen-specific domains which may be linked by disulfide bridges. They can be elicited by haptens or other antigens and may be mass-produced by hybridomas or monoclones in the laboratory.

Genetically identical individuals developed from brother and sister matings which have been carried out for twenty or more generations, or by parent x offspring matings carried out with certain restrictions. All animals within an inbred strain trace back to a common ancestor in the twentieth generation.

An immunoassay utilizing an antibody labeled with an enzyme marker such as horseradish peroxidase. While either the enzyme or the antibody is bound to an immunosorbent substrate, they both retain their biologic activity; the change in enzyme activity as a result of the enzyme-antibody-antigen reaction is proportional to the concentration of the antigen and can be measured spectrophotometrically or with the naked eye. Many variations of the method have been developed.

Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.

Established cell cultures that have the potential to propagate indefinitely.

An encapsulated lymphatic organ through which venous blood filters.

A soluble substance elaborated by antigen- or mitogen-stimulated T-LYMPHOCYTES which induces DNA synthesis in naive lymphocytes.

A class of immunoglobulin bearing mu chains (IMMUNOGLOBULIN MU-CHAINS). IgM can fix COMPLEMENT. The name comes from its high molecular weight and originally being called a macroglobulin.

Allelic variants of the immunoglobulin light chains (IMMUNOGLOBULIN LIGHT CHAINS) or heavy chains (IMMUNOGLOBULIN HEAVY CHAINS) encoded by ALLELES of IMMUNOGLOBULIN GENES.

The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.

T-cell enhancement of the B-cell response to thymic-dependent antigens.

The processes triggered by interactions of ANTIBODIES with their ANTIGENS.

The major immunoglobulin isotype class in normal human serum. There are several isotype subclasses of IgG, for example, IgG1, IgG2A, and IgG2B.

Substances that are recognized by the immune system and induce an immune reaction.

The class of heavy chains found in IMMUNOGLOBULIN M. They have a molecular weight of approximately 72 kDa and they contain about 57 amino acid residues arranged in five domains and have more oligosaccharide branches and a higher carbohydrate content than the heavy chains of IMMUNOGLOBULIN G.

The complex formed by the binding of antigen and antibody molecules. The deposition of large antigen-antibody complexes leading to tissue damage causes IMMUNE COMPLEX DISEASES.

A heterogeneous group of immunocompetent cells that mediate the cellular immune response by processing and presenting antigens to the T-cells. Traditional antigen-presenting cells include MACROPHAGES; DENDRITIC CELLS; LANGERHANS CELLS; and B-LYMPHOCYTES. FOLLICULAR DENDRITIC CELLS are not traditional antigen-presenting cells, but because they hold antigen on their cell surface in the form of IMMUNE COMPLEXES for B-cell recognition they are considered so by some authors.

The genetic region which contains the loci of genes which determine the structure of the serologically defined (SD) and lymphocyte-defined (LD) TRANSPLANTATION ANTIGENS, genes which control the structure of the IMMUNE RESPONSE-ASSOCIATED ANTIGENS, HUMAN; the IMMUNE RESPONSE GENES which control the ability of an animal to respond immunologically to antigenic stimuli, and genes which determine the structure and/or level of the first four components of complement.

A hapten capable of eliciting both antibody formation and delayed hypersensitivity when bound to aromatic amino acids, polypeptides or proteins. It is used as an immunologic research tool.

A measure of the binding strength between antibody and a simple hapten or antigen determinant. It depends on the closeness of stereochemical fit between antibody combining sites and antigen determinants, on the size of the area of contact between them, and on the distribution of charged and hydrophobic groups. It includes the concept of "avidity," which refers to the strength of the antigen-antibody bond after formation of reversible complexes.

The major group of transplantation antigens in the mouse.

Genetic loci in the vertebrate major histocompatibility complex that encode polymorphic products which control the immune response to specific antigens. The genes are found in the HLA-D region in humans and in the I region in mice.

The phenomenon of immense variability characteristic of ANTIBODIES. It enables the IMMUNE SYSTEM to react specifically against the essentially unlimited kinds of ANTIGENS it encounters. Antibody diversity is accounted for by three main theories: (1) the Germ Line Theory, which holds that each antibody-producing cell has genes coding for all possible antibody specificities, but expresses only the one stimulated by antigen; (2) the Somatic Mutation Theory, which holds that antibody-producing cells contain only a few genes, which produce antibody diversity by mutation; and (3) the Gene Rearrangement Theory, which holds that antibody diversity is generated by the rearrangement of IMMUNOGLOBULIN VARIABLE REGION gene segments during the differentiation of the ANTIBODY-PRODUCING CELLS.

Antibodies that react with self-antigens (AUTOANTIGENS) of the organism that produced them.

T-cell receptors composed of CD3-associated alpha and beta polypeptide chains and expressed primarily in CD4+ or CD8+ T-cells. Unlike immunoglobulins, the alpha-beta T-cell receptors recognize antigens only when presented in association with major histocompatibility (MHC) molecules.

The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.

Derivatives of phenylacetic acid. Included under this heading are a variety of acid forms, salts, esters, and amides that contain the benzeneacetic acid structure. Note that this class of compounds should not be confused with derivatives of phenyl acetate, which contain the PHENOL ester of ACETIC ACID.

Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.

The process by which antigen is presented to lymphocytes in a form they can recognize. This is performed by antigen presenting cells (APCs). Some antigens require processing before they can be recognized. Antigen processing consists of ingestion and partial digestion of the antigen by the APC, followed by presentation of fragments on the cell surface. (From Rosen et al., Dictionary of Immunology, 1989)

A neoplasm originating from thymic tissue, usually benign, and frequently encapsulated. Although it is occasionally invasive, metastases are extremely rare. It consists of any type of thymic epithelial cell as well as lymphocytes that are usually abundant. Malignant lymphomas that involve the thymus, e.g., lymphosarcoma, Hodgkin's disease (previously termed granulomatous thymoma), should not be regarded as thymoma. (From Stedman, 25th ed)

Proteins secreted by the prostate gland. The major secretory proteins from the human prostate gland include PROSTATE-SPECIFIC ANTIGEN, prostate-specific acid phosphatase, prostate-specific membrane antigen, and prostate-specific protein-94.

One of the types of light chain subunits of the immunoglobulins with a molecular weight of approximately 22 kDa.

Benzene derivatives which are substituted with three nitro groups in any position.

Ordered rearrangement of B-lymphocyte variable gene regions coding for the kappa or lambda IMMUNOGLOBULIN LIGHT CHAINS, thereby contributing to antibody diversity. It occurs during the second stage of differentiation of the IMMATURE B-LYMPHOCYTES.

The production of ANTIBODIES by proliferating and differentiated B-LYMPHOCYTES under stimulation by ANTIGENS.

Deliberate stimulation of the host's immune response. ACTIVE IMMUNIZATION involves administration of ANTIGENS or IMMUNOLOGIC ADJUVANTS. PASSIVE IMMUNIZATION involves administration of IMMUNE SERA or LYMPHOCYTES or their extracts (e.g., transfer factor, immune RNA) or transplantation of immunocompetent cell producing tissue (thymus or bone marrow).

Soluble protein factors generated by activated lymphocytes that affect other cells, primarily those involved in cellular immunity.

The classes of immunoglobulins found in any species of animal. In man there are nine classes that migrate in five different groups in electrophoresis; they each consist of two light and two heavy protein chains, and each group has distinguishing structural and functional properties.

Autoantibodies directed against various nuclear antigens including DNA, RNA, histones, acidic nuclear proteins, or complexes of these molecular elements. Antinuclear antibodies are found in systemic autoimmune diseases including systemic lupus erythematosus, Sjogren's syndrome, scleroderma, polymyositis, and mixed connective tissue disease.

Antibodies which react with the individual structural determinants (idiotopes) on the variable region of other antibodies.

Glycoproteins expressed on all mature T-cells, thymocytes, and a subset of mature B-cells. Antibodies specific for CD5 can enhance T-cell receptor-mediated T-cell activation. The B-cell-specific molecule CD72 is a natural ligand for CD5. (From Abbas et al., Cellular and Molecular Immunology, 2d ed, p156)

Abnormal immunoglobulins characteristic of MULTIPLE MYELOMA.

Antibodies elicited in a different species from which the antigen originated. These antibodies are directed against a wide variety of interspecies-specific antigens, the best known of which are Forssman, Hanganutziu-Deicher (H-D), and Paul-Bunnell (P-B). Incidence of antibodies to these antigens--i.e., the phenomenon of heterophile antibody response--is useful in the serodiagnosis, pathogenesis, and prognosis of infection and latent infectious states as well as in cancer classification.

Endogenous tissue constituents that have the ability to interact with AUTOANTIBODIES and cause an immune response.

Multi-subunit proteins which function in IMMUNITY. They are produced by B LYMPHOCYTES from the IMMUNOGLOBULIN GENES. They are comprised of two heavy (IMMUNOGLOBULIN HEAVY CHAINS) and two light chains (IMMUNOGLOBULIN LIGHT CHAINS) with additional ancillary polypeptide chains depending on their isoforms. The variety of isoforms include monomeric or polymeric forms, and transmembrane forms (B-CELL ANTIGEN RECEPTORS) or secreted forms (ANTIBODIES). They are divided by the amino acid sequence of their heavy chains into five classes (IMMUNOGLOBULIN A; IMMUNOGLOBULIN D; IMMUNOGLOBULIN E; IMMUNOGLOBULIN G; IMMUNOGLOBULIN M) and various subclasses.

A basic enzyme that is present in saliva, tears, egg white, and many animal fluids. It functions as an antibacterial agent. The enzyme catalyzes the hydrolysis of 1,4-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrin. EC 3.2.1.17.

Proteins, glycoprotein, or lipoprotein moieties on surfaces of tumor cells that are usually identified by monoclonal antibodies. Many of these are of either embryonic or viral origin.

Substances elaborated by viruses that have antigenic activity.

Ordered rearrangement of B-lymphocyte variable gene regions coding for the IMMUNOGLOBULIN CHAINS, thereby contributing to antibody diversity. It occurs during the differentiation of the IMMATURE B-LYMPHOCYTES.

Repertoire of human antibodies against the polysaccharide capsule of Streptococcus pneumoniae serotype 6B. (1/2670)

We examined the repertoire of antibodies to Streptococcus pneumoniae 6B capsular polysaccharide induced with the conventional polysaccharide vaccine in adults at the molecular level two ways. In the first, we purified from the sera of seven vaccinees antipneumococcal antibodies and determined their amino acid sequences. Their VH regions are mainly the products of VH3 family genes (candidate genes, 3-23, 3-07, 3-66, and 3-74), but the product of a VH1 family gene (candidate gene, 1-03) is occasionally used. All seven individuals have small amounts of polyclonal kappa+ antibodies (Vkappa1 to Vkappa4 families), although kappa+ antibodies are occasionally dominated by antibodies formed with the product of the A27 Vkappa gene. In contrast, lambda+ anti-6B antibodies are dominated by the antibodies derived from one of 3 very similar Vlambda2 family genes (candidate genes, 2c, 2e, and 2a2) and Clambda1 gene product. The Vlambda2(+) antibodies express the 8.12 idiotype, which is expressed on anti-double-stranded-DNA antibodies. In one case, Vlambda is derived from a rarely expressed Vlambda gene, 10a. In the second approach, we studied a human hybridoma (Dob1) producing anti-6B antibody. Its VH region sequence is closely related to those of the 3-15 VH gene (88% nucleotide homology) and JH4 (92% homology). Its VL region is homologous to the 2a2 Vlambda2 gene (91%) and Jlambda1/Clambda1. Taken together, the V region of human anti-6B antibodies is commonly formed by a VH3 and a Vlambda2 family gene product. (+info)

Mechanisms of double-strand-break repair during gene targeting in mammalian cells. (2/2670)

In the present study, the mechanism of double-strand-break (DSB) repair during gene targeting at the chromosomal immunoglobulin mu-locus in a murine hybridoma was examined. The gene-targeting assay utilized specially designed insertion vectors genetically marked in the region of homology to the chromosomal mu-locus by six diagnostic restriction enzyme site markers. The restriction enzyme markers permitted the contribution of vector-borne and chromosomal mu-sequences in the recombinant product to be determined. The use of the insertion vectors in conjunction with a plating procedure in which individual integrative homologous recombination events were retained for analysis revealed several important features about the mammalian DSB repair process:The presence of the markers within the region of shared homology did not affect the efficiency of gene targeting. In the majority of recombinants, the vector-borne marker proximal to the DSB was absent, being replaced with the corresponding chromosomal restriction enzyme site. This result is consistent with either formation and repair of a vector-borne gap or an "end" bias in mismatch repair of heteroduplex DNA (hDNA) that favored the chromosomal sequence. Formation of hDNA was frequently associated with gene targeting and, in most cases, began approximately 645 bp from the DSB and could encompass a distance of at least 1469 bp. The hDNA was efficiently repaired prior to DNA replication. The repair of adjacent mismatches in hDNA occurred predominantly on the same strand, suggesting the involvement of a long-patch repair mechanism. (+info)

The molecular basis of multiple vector insertion by gene targeting in mammalian cells. (3/2670)

Gene targeting using sequence insertion vectors generally results in integration of one copy of the targeting vector generating a tandem duplication of the cognate chromosomal region of homology. However, occasionally the target locus is found to contain >1 copy of the integrated vector. The mechanism by which the latter recombinants arise is not known. In the present study, we investigated the molecular basis by which multiple vectors become integrated at the chromosomal immunoglobulin mu locus in a murine hybridoma. To accomplish this, specially designed insertion vectors were constructed that included six diagnostic restriction enzyme markers in the Cmu region of homology to the target chromosomal mu locus. This enabled contributions by the vector-borne and chromosomal Cmu sequences at the recombinant locus to be ascertained. Targeted recombinants were isolated and analyzed to determine the number of vector copies integrated at the chromosomal immunoglobulin mu locus. Targeted recombinants identified as bearing >1 copy of the integrated vector resulted from a Cmu triplication formed by two vector copies in tandem. Examination of the fate of the Cmu region markers suggested that this class of recombinant was generated predominantly, if not exclusively, by two targeted vector integration events, each involving insertion of a single copy of the vector. Both vector insertion events into the chromosomal mu locus were consistent with the double-strand-break repair mechanism of homologous recombination. We interpret our results, taken together, to mean that a proportion of recipient cells is in a predetermined state that is amenable to targeted but not random vector integration. (+info)

Infrequent translation of a nonsense codon is sufficient to decrease mRNA level. (4/2670)

In many organisms nonsense mutations decrease the level of mRNA. In the case of mammalian cells, it is still controversial whether translation is required for this nonsense-mediated RNA decrease (NMD). Although previous analyzes have shown that conditions that impede translation termination at nonsense codons also prevent NMD, the residual level of termination was unknown in these experiments. Moreover, the conditions used to impede termination might also have interfered with NMD in other ways. Because of these uncertainties, we have tested the effects of limiting translation of a nonsense codon in a different way, using two mutations in the immunoglobulin mu heavy chain gene. For this purpose we exploited an exceptional nonsense mutation at codon 3, which efficiently terminates translation but nonetheless maintains a high level of mu mRNA. We have shown 1) that translation of Ter462 in the double mutant occurs at only approximately 4% the normal frequency, and 2) that Ter462 in cis with Ter3 can induce NMD. That is, translation of Ter462 at this low (4%) frequency is sufficient to induce NMD. (+info)

Soluble class I MHC with beta2-microglobulin covalently linked peptides: specific binding to a T cell hybridoma. (5/2670)

Soluble forms of the mouse MHC class I molecule, Dd, were produced in which the peptide binding groove was uniformly occupied by peptides attached via a covalent flexible peptide linker to the N terminus of the associated beta2-microglobulin. The MHC heavy chain and beta2-microglobulin were firmly associated, and the molecules displayed an Ab epitope requiring proper occupancy of the peptide binding groove. Soluble Dd containing a covalent version of a well-characterized Dd-binding peptide from HIV stimulated a T cell hybridoma specific for this combination. Furthermore, a tetravalent version of this molecule bound specifically with apparent high avidity to this hybridoma. (+info)

Cloning, expression, and characterization of the Fab fragment of the anti-lysozyme antibody HyHEL-5. (6/2670)

Hybridoma cDNAs encoding the individual chains of the Fab fragment of the well characterized murine monoclonal antibody HyHEL-5 were cloned and sequenced. The recombinant Fab fragment was produced by expressing each chain in a separate Escherichia coli pET vector, denaturing inclusion bodies and co-refolding. Characterization of the purified Fab by MALDI-TOF mass spectrometry and N-terminal amino acid sequencing demonstrated proper processing of the individual chains. The association of the recombinant Fab fragment with hen egg lysozyme and the avian epitope variant bobwhite quail lysozyme was found by isothermal titration calorimetry to have energetics very similar to that of the HyHEL-5 IgG. Heterologous expression of the HyHEL-5 Fab fragment opens the way to structure/function studies in this well-known system. (+info)

A novel 62-kilodalton egg antigen from Schistosoma mansoni induces a potent CD4(+) T helper cell response in the C57BL/6 mouse. (7/2670)

In infection with Schistosoma mansoni, hepatic granuloma formation is mediated by CD4(+) T helper (Th) cells sensitized to schistosomal egg antigens. There is considerable variation among infected individuals with respect to both severity of disease and the T-cell response to egg antigens. In the BL/6 mouse, the egg granulomas are relatively small and the relevant sensitizing egg antigens are largely unknown. We investigated the CD4(+) Th cell response of infected BL/6 mice to egg antigens fractionated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and found a prominent lymphoproliferative response to be directed against a 62-kDa component. With the aid of a specific T-cell hybridoma, 4E6, the 62-kDa antigen was isolated; following partial digestion with endoproteinase Glu-C, an internal amino acid sequence was found to be identical with one present in the enzyme phosphoenolpyruvate carboxykinase (PEPCK) of the organisms Caenorhabditis elegans and Treponema pallidum and to differ by one residue from PEPCK of various other species. In CD4(+) Th cells from 7.5- 8.5-week-infected BL/6 mice, the purified 62-kDa molecule elicited a potent proliferative response which, based on cytokine analysis, was of a mixed Th-1 and Th-2 type. Our results reveal a novel egg antigen of particular prominence in the BL/6 mouse and suggest that the immune response in schistosomiasis is a product of sensitization to egg antigens that may vary considerably in immunogenicity from strain to strain. (+info)

Immunosuppressant deoxyspergualin-induced inhibition of cell proliferation is accompanied with an enhanced reduction of tetrazolium salt. (8/2670)

Deoxyspergualin (DSG) has both antitumor and immunosuppressive activities. We explored the mechanism of DSG activities using an aqueous soluble analogue, methyldeoxyspergualin (MeDSG) for in vitro culture studies. It is known that DSG has inhibitory effects on cell proliferation, and we also observed that MeDSG inhibited [3H]-thymidine incorporation by rapidly dividing murine T cell hybridomas. However, when tetrazolium (MTT) colorimetric assay was adopted to evaluate its inhibitory effects on cell proliferation, MeDSG induced an enhanced MTT reduction. When we examined whether these results were applicable to the actively dividing cells of other origins than T cells, similar effects were seen with Raji cells, J774.1 cells and NIH3T3 cells. N-30, another analogue which was capable of suppressing anti-SRBC antibody production in vivo, also induced inhibition of cell growth and an enhanced MTT reduction. In contrast, the analogue which failed to prevent the antibody production, neither enhanced MTT reduction nor inhibited cell proliferation. Our results demonstrated that the ability to generate MTT formazan in dividing cells is a common property among, DSG analogue with the immunosuppressive and antiproliferative activities. (+info)

Antibodies Hybridomas. 3 (2): 65-74. doi:10.3233/HAB-1992-3202. PMID 1633267. Roes J, Rajewsky K (1992). "Cell autonomous ...

https://en.wikipedia.org/wiki/IGHD

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https://en.wikipedia.org/wiki/LSP1

Hybridoma. 19 (5): 375-385. doi:10.1089/02724570050198893. ISSN 0272-457X. PMID 11128027. Li, Y.; Foss, C. A.; Summerfield, D. ...

https://en.wikipedia.org/wiki/Collagen_hybridizing_peptide

Hybridoma. 28 (4): 251-7. doi:10.1089/hyb.2009.0017. PMID 19663697. Palumbo KS, Wands JR, Safran H, King T, Carlson RI, de la ...

https://en.wikipedia.org/wiki/ASPH

1986). "Pancreatic amylase expression in human pancreatic development". Hybridoma. 5 (2): 137-45. doi:10.1089/hyb.1986.5.137. ...

https://en.wikipedia.org/wiki/AMY1A

Hybridoma. 11 (1): 33-9. doi:10.1089/hyb.1992.11.33. PMID 1737638. Bolstad N, Warren DJ, Nustad K (October 2013). "Heterophilic ...

https://en.wikipedia.org/wiki/Heterophile_antibody

Hybridoma. 17 (5): 431-5. doi:10.1089/hyb.1998.17.431. PMID 9873988. Enright, Helen (Oct 1995). "Paraneoplastic autoimmune ...

https://en.wikipedia.org/wiki/Anti-actin_antibodies

Hybridoma. 20 (3): 159-66. doi:10.1089/027245701750293484. PMID 11461664. Yoon HG, Chan DW, Reynolds AB, Qin J, Wong J (Sep ...

https://en.wikipedia.org/wiki/ZBTB33

Hybridoma. 20 (3): 149-57. doi:10.1089/027245701750293475. PMID 11461663.. ...

https://en.wikipedia.org/wiki/Camptodactyly-arthropathy-coxa_vara-pericarditis_syndrome

Hybridoma. 26 (4): 231-40. doi:10.1089/hyb.2007.0507. PMID 17725385. Stecca B, Mas C, Ruiz i Altaba A (May 2005). "Interference ...

https://en.wikipedia.org/wiki/Hedgehog_signaling_pathway

Hybridoma and Hybridomics. 21 (1): 1-10. doi:10.1089/15368590252917584. PMID 11991811. Bromidge T, Lynas C (Jul 2002). " ...

https://en.wikipedia.org/wiki/ING1

The National Centre for Cell Science (NCCS), Pune, India; national repository for cell lines/hybridomas etc. Public Health ... to produce a hybridoma which has the antibody specificity of the primary lymphocyte and the immortality of the myeloma. ...

https://en.wikipedia.org/wiki/Cell_culture

1999) Hybridoma 18: 343-349. 35. Daniel JM and Reynolds AB. The catenin p120ctn interacts with Kaiso, a novel BTB/POZ domain ... 2001) Hybridoma 20: 159-166. 33. Thoreson MA, Anastasiadis PZ, Daniel JM, Ireton RC, Wheelock MJ, Johnson KR, Hummingbird DK ...

https://en.wikipedia.org/wiki/Juliet_Daniel

Soll is now the Director of the Developmental Studies Hybridoma Bank (DSHB), a NIH National Resource, Director of the W.M. Keck ... He is the current director of the Developmental Studies Hybridoma Bank, Monoclonal Antibody Research Institute, and the WM Keck ... Developmental Studies Hybridoma Bank WM. Keck Dynamic Image Analysis Facility. ...

https://en.wikipedia.org/wiki/David_R._Soll

Adekar SP, Jones RM, Elias MD, Al-Saleem FH, Root MJ, Simpson LL, Dessain SK (2008) "Hybridoma populations enriched for ... CS1 maint: discouraged parameter (link) "Human IgA Monoclonal Antibodies That Neutralize Poliovirus, Produced by Hybridomas and ... "High efficiency creation of human monoclonal antibody-producing hybridomas." J Immunol Methods 291 (1-2): 109-22. ...

https://en.wikipedia.org/wiki/Scott_K._Dessain

This process is called hybridoma technology. Laboratory animals (e.g., mice) are first exposed to an antigen against which we ...

https://en.wikipedia.org/wiki/HAT_medium

"Organization and expression of immunoglobulin genes in fetal liver hybridomas". Proceedings of the National Academy of Sciences ...

https://en.wikipedia.org/wiki/Organization_and_expression_of_immunoglobulin_genes

"Activation-induced cytidine deaminase turns on somatic hypermutation in hybridomas". Nature. 415 (6873): 802-6. doi:10.1038/ ...

https://en.wikipedia.org/wiki/Activation-induced_cytidine_deaminase

The hybridomas can be grown indefinitely in a suitable cell culture medium. They can also be injected into mice (in the ... The medium must be enriched during in vitro selection to further favour hybridoma growth. This can be achieved by the use of a ... Rabbit B-cells can be used to form a rabbit hybridoma. Polyethylene glycol is used to fuse adjacent plasma membranes, but the ... Only fused hybrid cells referred to as hybridomas, are able to grow indefinitely in the medium because the spleen cell partner ...

https://en.wikipedia.org/wiki/Monoclonal_antibody

"Cloning by Limiting Dilution of Hybridoma".. ...

https://en.wikipedia.org/wiki/Dilution_cloning

For production of monoclonal antibodies by producing hybridoma. For production of Induced stem cells. To assess protein ...

https://en.wikipedia.org/wiki/Cell_fusion

Milstein, C (1999). "The hybridoma revolution: an offshoot of basic research.". BioEssays 21 (11): 966-73. doi:10.1002/(SICI) ...

https://pt.wikipedia.org/wiki/César_Milstein

"Activation-induced cell death in T cell hybridomas is due to apoptosis. Morphologic aspects and DNA fragmentation". Journal of ... "Role for c-myc in activation-induced apoptotic cell death in T cell hybridomas". Science. 257 (5067): 212-4. doi:10.1126/ ... "Cyclosporin a inhibits activation-induced cell death in T-cell hybridomas and thymocytes". Nature. 339 (6226): 625-6. doi: ...

https://en.wikipedia.org/wiki/Douglas_R._Green

In 1973 he described, with Edward Cohen, a method of producing antibodies involving human-mouse hybrid cells, or hybridomas. ... In 1975, Georges Köhler, César Milstein, and Niels Kaj Jerne, succeeded in making hybridomas that made antibodies to known ... the beginnings of hybridoma technology". Journal of the History of Biology 25 (2): 175-230. doi:10.1007/BF00162840. PMID ...

https://en.wikipedia.org/wiki/Jerrold_Schwaber

El Debs B, Utharala R, Balyasnikova IV, Griffiths AD, Merten CA (July 2012). "Functional single-cell hybridoma screening using ...

https://en.wikipedia.org/wiki/Droplet-based_microfluidics

He JL, Dai D, Zhou N, Zhou JY (2012) Analysis of Putative ORF3 Gene Within Porcine Circovirus Type 2. Hybridoma (Larchmt) 31(3 ...

https://en.wikipedia.org/wiki/Porcine_circovirus

Antibodies come from animal sources (or animal sourced hybridoma cultures). The secondary antibody recognises and binds to the ...

https://en.wikipedia.org/wiki/Western_blot

who isolated IL17A transcript from a rodent T-cell hybridoma. The protein encoded by IL17A is a founding member of IL-17 family ...

https://en.wikipedia.org/wiki/Interleukin_17

At first, mouse hybridoma cells whose monoclonal antibodies target one of the desired antigens are produced. Independently, rat ... These two cell types are hybridised, yielding hybrid-hybridomas or quadromas, which produce hybrid (trifunctional) antibody as ... hybridoma cells targeting the other antigen are produced. ...

https://en.wikipedia.org/wiki/Trifunctional_antibody

SeV's ability to fuse eukaryotic cells and to form syncytium was used to produce hybridoma cells capable of manufacturing ... and a myeloma tumor cell to produce hybridomas, capable of being grown indefinitely and of producing significant amounts of an ... "Cell surface sialic acid and the invasive and metastatic potential of T-cell hybridomas". Cancer Research. 46 (7): 3521-7. PMID ...

https://en.wikipedia.org/wiki/Murine_respirovirus

Some hybridomas in the ATCC collection are somatic cell hybrids. These cells are capable of producing immunoglobulins that are ... Hybridomas are immortalized cells derived from the fusion of B lymphoblasts with a myeloma fusion partner. ... Alphanumeric - Hybridomas Search a wide selection of hybridomas alphanumerically. Mouse Hybridomas Search for hybridomas by ... Hybridomas Hybridomas are immortalized cells derived from the fusion of B lymphoblasts with a myeloma fusion partner. Some ...

https://www.atcc.org/en/Products/Cells_and_Microorganisms/Hybridomas.aspx

... known as a hybridoma, multiplies rapidly in culture. Since the antibodies obtained from hybridomas are produced by clones ... hybrid cell, known as a hybridoma, multiplies rapidly in culture. Since the antibodies obtained from hybridomas are produced by ... As shown in step 4, only hybridomas can live in the HAT medium; unfused myeloma cells, lacking HGPRT, die in the medium, as do ... The resulting hybrid cells, called hybridomas, grow at the rate of myeloma cells but also produce large amounts of the desired ...

https://www.britannica.com/science/hybridoma

Hybridoma fusion and selection reagents are designed to assist fusion and select hybridomas from among normal cell populations ... Need Help Finding Hybridomas? View a comprehensive listing of ATCC hybridomas by antigenic determinant. ... Hybridoma Development * RPC5.4 (ATCC® TIB-12™) ATCC® Number: TIB-12™ Organism: Mus musculus, mouse ... PEG-4000 is used to assist in the fusion of myeloma and spleen cells to form hybridomas. ...

https://atcc.org/en/Products/Culture_Reagents/Hybridoma_Development.aspx?dsNav=N:4294967226

https://atcc.org/Products/Culture_Reagents/Hybridoma_Development.aspx?dsNav=Nrc:id-1000506-dynrank-disabled,N:4294922650&hasShowAll=1000506%7C

The hybridomas can be grown in culture, each culture starting with one viable hybridoma cell, producing cultures each of which ... Hybridomas at the US National Library of Medicine Medical Subject Headings (MeSH) "Hybridoma Technology". Understanding Cancer ... Once a hybridoma colony is established, it will continually grow in culture medium like RPMI-1640 (with antibiotics and fetal ... Hybridoma technology is a method for producing large numbers of identical antibodies (also called monoclonal antibodies). This ...

https://en.wikipedia.org/wiki/Hybridoma_technology

A rabbit hybridoma is a hybrid cell line formed by the fusion of an antibody producing rabbit B cell with a cancerous B-cell ( ... "Recombinant human interleukin-6 enhances the immunoglobulin secretion of a rabbit-rabbit hybridoma". Hybridoma. 20 (3): 189-98 ... The process of hybridoma formation in a rabbit first entails obtaining B-cells from a rabbit that has been immunized. There are ... Resulting antibodies from hybridomas are screened for an antigen which meets criteria of interest by diagnostic tests such as ...

https://en.wikipedia.org/wiki/Rabbit_hybridoma

... hybridoma technology to the production of human monoclonal antibodies, human lymphoid lines producing antibody with defined... ... Weiss, R. A., 1982, Hybridomas produce viruses as well as antibodies, Immunol. Today 3: 292-294.CrossRefGoogle Scholar ... Kozbor, D., and Roder, J., 1984, In vitro stimulated lymphocytes as a source of human hybridomas, Eur. J. Immunol., 14: 23-27. ... Milstein, C., and Cuello, A. C., 1983, Hybrid hybridomas and their use in immunohistochemistry, Nature 305: 537-540.PubMed ...

https://link.springer.com/chapter/10.1007/978-1-4684-4949-5_4

... ,Perhaps sheer in-house demand is causing shortages in your laboratory or fellow researchers, ... Mouse Anti-Drosophila fasciclin III Monoclonal Antibody, Unconjugated from Developmental Studies Hybridoma Bank. 10. Hybridoma ... Hybridoma Development from QED Bioscience Inc.. 2. Mouse Anti-Xenopus laevis Xenopus nuclear factor Antibody, Unconjugated from ... Mouse Anti-Quail cell marker Antibody, Unconjugated from Developmental Studies Hybridoma Bank. 7. Mouse Anti-Human VCAM-1 ...

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SECRETING HUMAN MYELOMA CELL LINE WILL BE DEVELOPED AND CHARACTERIZED FOR SPECIFIC APPLICATION TO HUMAN HYBRIDOMA PRO ... ...

https://www.sbir.gov/sbirsearch/detail/183744

The use of chitosan fibers as a matrix for immobilizing hybridoma cells was investigated. Optimal cell entrapment within ... Phillips, H.A., Scharer, J.M., Bols, N.C., Moo-Young, M. (1987). Effect of Oxygen on Antibody Production in Hybridoma Culture. ... Buehler, R.J. (1984). Production of monoclonal antibody from gel entrapped hybridoma cells.Karyon Technology News, 1, p 3. ... Chitosan fibers were found to be more effective than chitosan beads in the occlusion of the hybridoma cells. Maximum total cell ...

https://link.springer.com/article/10.1007/BF01876164

U-M Hybridoma Core The UM-Hybridoma Core research laboratory was established in 1980 under the leadership of the Michigan ... The UM-Hybridoma Core generates somatic-cell hybrids (hybridomas) that produce monoclonal antibodies of desired specificity. ... Hybridoma Core services are available to all University of Michigan investigators as well as to other institutions and the ... The Hybridoma Core has developed monoclonal antibodies against a wide variety of lymphocyte surface antigens, tumor cell ...

https://www.med.umich.edu/hybridoma/index.htm

Bovine x mouse hybridomas that secrete bovine immunoglobulin G1 Message Subject. (Your Name) has forwarded a page to you from ... The interspecific fusion of normal bovine lymphocytes with a nonsecreting mouse hybridoma produced stable cell lines secreting ...

http://science.sciencemag.org/content/220/4596/522

Hybridoma cell lines developed by Covance remain exclusive property of the client. A typical hybridoma development proje, ... Covance has generated hybridomas against a large variety of antigens. Prior to project initiation, you are provided with ... Hybridoma cell lines developed by Covance remain exclusive property of the client. A typical hybridoma development project has ... Mouse Anti-Xenopus laevis Xenopus nuclear factor Antibody, Unconjugated from Developmental Studies Hybridoma Bank. 3. Hybridoma ...

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Opti-Clone II - Hybridoma Cloning Factor. Hybridoma Cloning Factor. MPs Opti-Clone™ is a partially purified hybridoma growth ... 50X Concentrate)Hypoxanthine 13.61 mg/LAminopterin 2H2O 0.1906 mg/LThymidine 3.876 mg/LSelective for hybridoma cell growth and ...

https://www.mpbio.com/index.php?cPath=7_43_379_844&country=223

... chemically defined medium optimized for the growth of a variety of hybridomas and myelomas and the production of monoclonal ... CD Hybridoma Medium✝. CD Hybridoma AGT™✝✝. Hybridoma-SFM✝. PFHM-II. Media Type. Chemically-Defined Media. Chemically-Defined ... Hybridoma-SFM Serum-free formulation Hybridoma-SFM is a very low-protein medium (20 μg/mL) optimized for the growth of human, ... A-Gibco® CD Hybridoma Medium B-Gibco® IMDM+10%FBS C-Gibco® Hybridoma-SFM D, E and F are media from other manufacturers. ...

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HCGS),br,,B,From Cultured Human Endothelial Cells,/B,,br,HCGS will significantly improve the hybridoma cell cloning efficiency ... Hybridoma cell growth supplement, 25 mL. CN¥0.00 Key features and details. Hybridoma Cell Growth Supplement (HCGS) from ... HCGS will significantly improve the hybridoma cell cloning efficiency while eliminating feeder cell need.. HCGS may be used in ... cultured human endothelial cells will significantly improve the hybridoma cell cloning efficiency while eliminating feeder cell ...

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Early History of Specific Antibody-producing Lymphocyte Hybridomas Message Subject (Your Name) has forwarded a page to you from ...

http://cancerres.aacrjournals.org/content/41/3/1246

After fusion and selection of 54 HAT-resistant hybridomas, three clones have been obtained (1F1, 2H4 and 4D7) that secrete ... Sample records for hybridomas. from WorldWideScience.org. Sample records 1 - 1 shown. ... hybridoma supernatants) and 1:20.000 (ascitis fluid). The three MAbs showed a wide spectrum of reactivity, recognizing antigens ...

https://worldwidescience.org/topicpages/multi/PT/h/hybridomas.html

Would anyone have any information on a hybridoma protocol which specifically targets IgM antibodies? I read that they appear ... IgM hybridoma protocol - posted in Immunology: Hello all, We are looking to generate an IgM antibody against our protein of ... Of MAJOR importance in obtaining a good number of hybridomas is the quality of your FCS. We always pre-screen batches by ... IgM hybridoma protocol. Started by TonyL, Jan 26 2009 04:21 PM ... Why do you would obtain an IgM hybridoma?? The IgG monoclonal ...

http://www.protocol-online.org/forums/topic/5932-igm-hybridoma-protocol/

Screening hybridoma libraries for antibodies that bind to cell surface antigens is a time and resource intensive process. The ... Screening hybridoma libraries for antibodies that bind to cell surface antigens is a time and resource intensive process. The ... Application Note: Mix-and-Measure Assay for Screening Hybridoma Supernatants on IntelliCyt Platforms. 9 November 2018 ...

https://www.selectscience.net/application-articles/mix-and-measure-assay-for-screening-hybridoma-supernatants-on-intellicyt-platforms?artID=47631

For instance, the T cell hybridoma that recognizes SIINFEKL/Kb - cant find the hybridoma for sale anywhere. ... I cant seem to find a vendor that sells T cell hybridomas that I know exist. ... Where do you purchase T cell hybridomas - posted in Immunology: ... Where do you purchase T cell hybridomas. Started by Ahrenhase, ... I cant seem to find a vendor that sells T cell hybridomas that I know exist. For instance, the T cell hybridoma that ...

http://www.protocol-online.org/forums/topic/33785-where-do-you-purchase-t-cell-hybridomas/

Immunoregulatory lymphokines of T hybridomas from AIDS patients: constitutive and inducible suppressor factors ... Immunoregulatory lymphokines of T hybridomas from AIDS patients: constitutive and inducible suppressor factors ... Immunoregulatory lymphokines of T hybridomas from AIDS patients: constitutive and inducible suppressor factors ... Immunoregulatory lymphokines of T hybridomas from AIDS patients: constitutive and inducible suppressor factors ...

http://science.sciencemag.org/content/225/4657/66

Similar words for Hybridoma. Definition: noun. a hybrid cell resulting from the fusion of a lymphocyte and a tumor cell; used ... 1. hybridoma noun. a hybrid cell resulting from the fusion of a lymphocyte and a tumor cell; used to culture a specific ...

https://www.synonym.com/synonyms/hybridoma

... Kang Liu kang.liu at worldnet.att.net Tue Sep 14 22:59:59 EST 1999 * ... I am trying to CD8 T cells from OT-I transgenic mouse (using T cell proliferation assay)and B3Z T-T hybridoma (measure IL-2 ... Next message: T cells from Transgenic TCR mouse v.s. T-T hybridoma ... Next message: T cells from Transgenic TCR mouse v.s. T-T hybridoma ...

http://www.bio.net/bionet/mm/immuno/1999-September/014784.html

Hybridoma,Production,Performance,medicine,medical news today,latest medical news,medical newsletters,current medical news, ... 1Pettit S, Santos M, Tanner T, Huang N. Enhanced Growth and Productivity of a Hybridoma with Recombinant Human Albumin and ... To obtain a copy of the above-mentioned article entitled, Enhanced Growth and Productivity of a Hybridoma with Recombinant ... This research supports what customers have been reporting from their use of Cellastim and Lacromin for hybridoma cell culture ...

http://www.bio-medicine.org/medicine-news-1/InVitria-Reveals-Technology-to-Optimize-Hybridoma-Production-Performance-43244-1/

BD Biosciences provides flow cytometers, reagents, tools, and a wide range of services to support the work of researchers and clinicians who understand disease and improve care ...

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This review aims at discussing the reasons for hybridoma-derived idiotype vaccines, more difficult to produce but also more ... Most published trials based on hybridoma-derived idiotype vaccines are being analyzed, together with the yet non-peer reviewed ... All in all, the sole randomized trial ever conducted on hybridoma-derived idiotype vaccines failed to achieve its primary ... Hybridoma-derived idiotype vaccines have been used for the experimental treatment of human lymphoma over the last twenty years ...

http://www.mdpi.com/1424-8247/3/3/667/notes

Hybridomas and monoclonal antibodies : presented at the Thirty-Fourth Annual Meeting of the American Association of Blood Banks ... schema:name "Hybridomas and monoclonal antibodies : presented at the Thirty-Fourth Annual Meeting of the American Association ... Hybridomas and monoclonal antibodies : presented at the Thirty-Fourth Annual Meeting of the American Association of Blood Banks ... Hybridomas and monoclonal antibodies : presented at the Thirty-Fourth Annual Meeting of the American Association of Blood Banks ...

http://www.worldcat.org/title/hybridomas-and-monoclonal-antibodies-presented-at-the-thirty-fourth-annual-meeting-of-the-american-association-of-blood-banks-november-3-1981/oclc/12132275

Our Magic-HybridomaTM Platform allows generation of 50-200 mouse hybridoma clones per hybridoma cell fusion and relies on the ... Our Magic Hybridoma™ Platform allows generation of 50-200 mouse hybridoma clones per hybridoma cell fusion. This platform ... Magic Hybridoma™ Technology. Magic Hybridoma™ Technology. "Creative Biolabs is committed to providing highly customized ... Therefore, Magic Hybridoma™ Platform represents an revolutionary method to produce a large number of monoclonal antibodies for ...

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... a human-murine hybridoma suitable as a fusion partner for immortalizing an antibody-secreting B cell has been generated. The ... The human-murine hybridoma (the "immortalizing hybridoma") is an immortal cell line which results from the fusion of a murine ... "Non-secreting" hybridoma refers to a hybridoma which is capable of continuous reproduction and, therefore, is immortal, which ... A hybridoma "having human characteristics" refers to a hybridoma which retains detectable human-derived chromosomes such as ...

http://www.freepatentsonline.com/4634666.html

Since the antibodies obtained from hybridoma s are produced by clones derived from a single lymphocyte, they are called monoclonal antibodies. (britannica.com)
Hybridoma technology is a method for producing large numbers of identical antibodies (also called monoclonal antibodies). (wikipedia.org)
The hybridomas can be grown in culture, each culture starting with one viable hybridoma cell, producing cultures each of which consists of genetically identical hybridomas which produce one antibody per culture (monoclonal) rather than mixtures of different antibodies (polyclonal). (wikipedia.org)
In contrast to polyclonal antibodies, which are mixtures of many different antibody molecules, the monoclonal antibodies produced by each hybridoma line are all chemically identical. (wikipedia.org)
The next stage is a rapid primary screening process, which identifies and selects only those hybridomas that produce antibodies of appropriate specificity. (wikipedia.org)
Once a hybridoma colony is established, it will continually grow in culture medium like RPMI-1640 (with antibiotics and fetal bovine serum) and produce antibodies. (wikipedia.org)
Several efforts were made to generate rabbit monoclonal antibodies after the development of mouse hybridoma technology in the 1970s. (wikipedia.org)
Research was conducted into mouse-rabbit hetero-hybridomas to make rabbit monoclonal antibodies. (wikipedia.org)
Fusion of 240E-1 cells with rabbit lymphocytes produced hybridomas that secreted rabbit monoclonal antibodies in a consistent manner. (wikipedia.org)
Resulting antibodies from hybridomas are screened for an antigen which meets criteria of interest by diagnostic tests such as ELISA, Western blot, Immunohistochemistry and FACS. (wikipedia.org)
Several years before the application of Köhler and Milstein's (1975) hybridoma technology to the production of human monoclonal antibodies, human lymphoid lines producing antibody with defined antigenic specificity were established by Epstein-Barr virus (EBV) "immortalization" (Steinitz et al . (springer.com)
The UM-Hybridoma Core generates somatic-cell hybrids (hybridomas) that produce monoclonal antibodies of desired specificity. (umich.edu)
The Hybridoma Core has developed monoclonal antibodies against a wide variety of lymphocyte surface antigens, tumor cell antigens, purified proteins, hormones, hormone receptors and recombinant proteins. (umich.edu)
Consultation is available from the Hybridoma Core Director on the design of immunization strategies and screening assays to ensure efficient detection of the desired monoclonal antibodies. (umich.edu)
Gibco® CD Hybridoma Medium is a chemically defined, protein-free medium optimized for the production of monoclonal antibodies and recombinant proteins in human, mouse, and rat hybridomas and myelomas. (thermofisher.com)
PFHM-II is a protein-free medium optimized for the growth of human, mouse, and rat hybridomas and myelomas, and the production of monoclonal antibodies. (thermofisher.com)
After fusion and selection of 54 HAT-resistant hybridomas, three clones have been obtained (1F1, 2H4 and 4D7) that secrete IgG2a antibodies reactin (mais) g to BoHV-1 antigens. (worldwidescience.org)
Would anyone have any information on a hybridoma protocol which specifically targets IgM antibodies? (protocol-online.org)
The IgG monoclonal antibodies will have, always, more affinity, more specificity, and more antibody concentration than an IgM hybridoma secreting cell. (protocol-online.org)
Screening hybridoma libraries for antibodies that bind to cell surface antigens is a time and resource intensive process. (selectscience.net)
Therefore, Magic Hybridoma™ Platform represents an revolutionary method to produce a large number of monoclonal antibodies for each target. (creative-biolabs.com)
We frequently obtain over 50-200 hybridoma clones per fusion, thus providing the solid basis to isolate functional antibodies. (creative-biolabs.com)
Thus, a cloned hybridoma cell line has an unlimited capacity to grow in culture, producing immunoglobulin molecules with identical structure and antigen specificity, termed monoclonal antibodies (mAbs), which may have a wide variety of applications. (upf.edu)
New York, US-Mar 13, 2019 With abundant experience in recombinant antibody discovery and manufacturing, Creative Biolabs has been committed to the service about high-quality monoclonal antibodies by the hybridoma technology. (digitaljournal.com)
In general, the mAb is prepared by use of the hybridomas antibodies technology. (digitaljournal.com)
Based on the cell fusion technology, this technology allows for the fusion of sensitized B cells with the ability to secrete specific antibodies and myeloma cells capable of reproducing indefinitely, resulting in the production of B cell hybridoma. (digitaljournal.com)
On top of the hybridoma technology, Creative Biolabs also excels in the human hybridoma antibodies production using transgenic mice. (digitaljournal.com)
Besides, the transgenic technology also contributes to the discovery and development of hybridoma antibodies. (digitaljournal.com)
Hybridomas Producing Highly Specific Monoclonal Antibodies to Detect Mycobacterium Avium Subspecies Paratuberculosis" was invented by John P. Bannantine (Ames, Iowa). (highbeam.com)
S. Patent & Trademark Office: "Hybridoma cell lines which produce and secrete monoclonal antibodies which selectively bind to Mycobacterium avium subspecies paratuberculosis have been produced. (highbeam.com)
Splenocytes from mice bearing NNK-specific antibodies were used to create hybridomas. (mdpi.com)
Selected fluorochrome conjugated antibodies from Miltenyi Biotec were compared to commercially available hybridoma clones in flow cytometry analysis. (miltenyibiotec.com)
The development of hybridomas allowed for the production of monoclonal antibodies at scale. (blogspot.com)
These days, hybridomas have been largely replaced by techniques permitting the generation of humanised or human antibodies that are safer when used as therapeutics. (blogspot.com)
None-the-less, patent law relating to therapeutic antibodies recognises the initial dependence of the field on hybridoma technology. (blogspot.com)
In T 0032/17 the Board of Appeal considered whether a product-by-process claim, directed to antibodies produced from a number of hybridomas, was novel in view of a prior reference to the hybridomas. (blogspot.com)
The patent application did not provide any information about the structure or sequence of the claimed antibodies other than by reference to these deposited hybridomas. (blogspot.com)
The mention of the hybridomas in the catalogue was cited as an alleged prior use of the claimed antibodies. (blogspot.com)
The Board of Appeal found that the deposit information provided in the application as filed for the hybridomas did not in fact convey any technical information about the structure of the antibodies produced by the hybridomas, either explicitly or implicitly. (blogspot.com)
The patentee also filed an auxiliary request including a product claim directed to the hybridomas per se as opposed to antibodies produced by the hybridomas. (blogspot.com)
Craig R. Halberstadt, Bernhard O. Palsson, A. Rees Midgley, and Rane L. Curl, "Optimization and Mathematical Modeling of the Transtubular Bioreactor for the Production of Monoclonal Antibodies from a Hybridoma Cell Line", Biotechnol. (umich.edu)
Absolute Antibody offers hybridoma sequencing services to protect valuable antibodies from mutation or loss, and to enable antibody engineering and recombinant expression. (nepic.co.uk)
The resulting hybridoma cultures were screened for the production of antibodies directed against the soybean protoplasts and were then cloned. (eurekamag.com)
Hybridomas secreting antigen-specific antibodies are cloned to establish a stable line secreting a unique antibody with defined specificity and isotype. (wustl.edu)
gammadelta T cell hybridomas derived from mice infected with Listeria were preferentially stimulated by low density macrophages from peritoneal exudates of infected mice.Furthermore, primary splenic gammadelta T cells isolated from Listeria-infected mice were cytotoxic for low density macrophages in vitro, and cytotoxicity was inhibited in the presence of antibodies to the gammadelta TCR. (nih.gov)
Role of outer membrane protein H (OmpH)- and OmpA-specific monoclonal antibodies from hybridoma tumors in protection of mice against Pasteurella multocida. (asm.org)
The backpack hybridoma tumor system in BALB/c mice was used to continuously deliver immunoglobulin G2b (IgG2b) monoclonal antibodies (MAbs) specific for OmpH (MAb MT1) and OmpA (MAb MT4.1). (asm.org)
We studied the V region genes of three IgM hybridoma monoclonal antibodies (BUD 45.12.8, BUD 114.4.11 and BUD 94.91.8) which were selected for Sm reactivity and derived from B cells of a healthy child. (uwo.ca)
Production of hybridomas which secrete specific monoclonal antibodies need several steps to complete. (cainscrossing.org)
Screening of the antibodies: Hybridomas need to screened for desired antibody specificity. (cainscrossing.org)
Monoclonal antibodies are commonly assumed to be monospecific, but anecdotal studies have reported genetic diversity in antibody heavy chain and light chain genes found within individual hybridomas. (elsevier.com)
These findings, reflecting the current state of hybridomas used in research, reiterate the importance of using sequence-defined recombinant antibodies for research or diagnostic use. (elsevier.com)
Serum-free media are commonly used in the production of monoclonal antibodies in hybridoma and recombinant myeloma cells. (capricorn-scientific.com)
The Hybridoma Facility will produce monoclonal antibodies to antigens provided by investigators. (yu.edu)
In general Dr. Scharff and Ms. Buhl will meet with the Principle Investigator, or his or her representative, to discuss the scientific problem and make decisions about how to immunize animals, to screen for positive hybridomas once a fusion is done and on the types of antibodies that are required. (yu.edu)

Hybridomas are immortalized cells derived from the fusion of B lymphoblasts with a myeloma fusion partner. (atcc.org)

Production of monoclonal antibody from gel entrapped hybridoma cells. (springer.com)

However, these hetero-hybridomas were ultimately difficult to clone, and the clones, generally unstable, and did not secrete antibody over a prolonged period of time. (wikipedia.org)
Our Magic Hybridoma™ Platform allows generation of 50-200 mouse hybridoma clones per hybridoma cell fusion. (creative-biolabs.com)
This platform relies on the novel immunization adjuvant, Magic Mouse Adjuvant, which enables generation of a large number of hybridoma clones in mice using even a trace amount of immunogen for most antigens. (creative-biolabs.com)
Among all research adjuvants and vaccine adjuvants available in the market, Magic Mouse Adjuvant by far is the most efficient mouse adjuvant for all types of immunogens tested, in terms of its rapidness in raising immune responses and the big number of hybridoma clones it can produce. (creative-biolabs.com)
The media are optimized to support the selection and growth of hybridoma clones using Molecular Devices ClonePix™ line of mammalian colony pickers, but they are also compatible with other appropriate methods. (moleculardevices.com)
The activation of the three latter T cell hybridoma clones with C57BL/6 splenocytes could be blocked completely by either anti-I-A or anti-L3T4 antibody. (jimmunol.org)
Up to 5 positive hybridoma clones will be delivered to customer in the cryotube format. (abnova.com)
Hybridomas were prepared from clones 34 and 59, and both reacted readily with T2 cells transfected with H-2Kb (data for hybridoma 34.9, derived from clone no. 34, shown in Fig. 9 A) and not with T2 cells transfected with the H-2Ld molecule (data not shown). (nih.gov)
Our Magic-Hybridoma™ Platform allows generation of 50-200 mouse hybridoma clones per hybridoma cell fusion step. (creative-diagnostics.com)
They are suitable for a variety of hybridoma clones. (capricorn-scientific.com)

The ultimate goal is to identify a single hybridoma cell producing a monoclonal antibody with a desired specificity or function. (moleculardevices.com)
Our hybridoma services boast many advantages, such as high specificity, high affinity, and high accuracy, novel epitope recognition, and comprehensive functional testing. (digitaljournal.com)
Acquisition of an additional antigen specificity after mouse CD4 gene transfer into a T helper hybridoma. (rupress.org)
Selection of the hybridomas: Hybridoma selection in HAT (Hypoxanthine, Aminopterine,Thymidine ) medium is followed by screening of the antibody specificity. (cainscrossing.org)
The most abundant mRNA transcripts found in a hybridoma cell line did not necessarily encode the antibody chains providing the correct specificity. (elsevier.com)

Suitable for the culture of recombinant myeloma lines as well as traditional hybridomas, CD Hybridoma Medium outperforms serum-free and serum-supplemented hybridoma media in terms of cell culture density and IgG production (Figure 1). (thermofisher.com)
Article in Spring edition of Bioprocessing Journal reports InVitria's recombinant human serum albumin (Cellastim) and recombinant lactoferrin (Lacromin) cell culture supplements optimize cell growth and productivity in hybridoma cells. (bio-medicine.org)
Cellastim has been shown to not only optimize hybridoma performance, but also outperform other sources of native and recombinant albumin. (bio-medicine.org)
To obtain a copy of the above-mentioned article entitled, 'Enhanced Growth and Productivity of a Hybridoma with Recombinant Human Albumin and Lactoferrin,' please contact InVitria at info(at)InVitria(dot)com or 1-800-916-8311. (bio-medicine.org)
1Pettit S, Santos M, Tanner T, Huang N. Enhanced Growth and Productivity of a Hybridoma with Recombinant Human Albumin and Lactoferrin. (bio-medicine.org)
Absolute Antibody Ltd. , an industry-leading provider of recombinant antibody products and services, today announced the availability of the SeqPack™ Hybridoma Preservation Kit, which streamlines the sample submission process for hybridoma sequencing customers worldwide. (nepic.co.uk)
In addition to its hybridoma sequencing service, Absolute Antibody offers antibody protein sequencing, antibody engineering, and recombinant expression, as well as a recombinant reagents catalog. (nepic.co.uk)
Based on type, the cell line development market is segmented into a primary cell line, hybridomas, continuous cell lines, and recombinant cell line. (marketstudyreport.com)

A typical hybridoma development project has four phases: immunization, fusion, cloning and hybridoma stabilization. (bio-medicine.org)
Hybridoma Cell Growth Supplement (HCGS) from cultured human endothelial cells will significantly improve the hybridoma cell cloning efficiency while eliminating feeder cell need. (mpbio.com)
HCGS will significantly improve the hybridoma cell cloning efficiency while eliminating feeder cell need. (mpbio.com)
Until recently, hybridoma-producing laboratories have pursued a labor-intensive and time-consuming serial path of hybridoma culturing and limiting dilution cloning to achieve clonality. (moleculardevices.com)
How is Hybridoma Cloning Factor abbreviated? (thefreedictionary.com)
The methylcellulose-based semi-solid Medium D contains the selective reagents hypoxanthine, aminopterin and thymidine (HAT), therefore combining HAT selection and cloning of hybridomas into a single step. (stemcell.com)
Using the standard protocol for selection and cloning of hybridomas in ClonaCell™-HY media, fused myeloma cells and splenocytes are suspended in selective semi-solid medium and incubated in 10 cm plates. (stemcell.com)
This method enables isolation of producing hybridomas with a high probability of monoclonality after only a single round of cloning. (stemcell.com)
On the day of fusion, place the hybridoma semi-solid cloning medium, ClonaCell™-HY Medium D (Catalog #03804), at 2 - 8°C to thaw overnight. (stemcell.com)
BriClone hybridoma cloning medium has been developed to overcome and eliminate these common problems. (qedbio.com)
Add BriClone Hybridoma Cloning Medium to hybridoma culture medium as a 5% (v/v) supplement. (qedbio.com)
BriClone is suitable for use in the post-fusion stages of hybridoma production and when cloning hybridoma cell lines under conditions of limiting dilution. (qedbio.com)
The hybridomas analyzed were not biased towards those with cloning difficulties or known to have additional chains. (elsevier.com)
Hybridoma Supplement is a completely defined growth supplement for fusion, selection and cloning of hybridoma cells. (capricorn-scientific.com)

The interspecific fusion of normal bovine lymphocytes with a nonsecreting mouse hybridoma produced stable cell lines secreting bovine immunoglobulins. (sciencemag.org)
Hybridomas are obtained by fusing normal B lymphocytes with a myeloma (tumor B cell line). (upf.edu)
Stabilizing antibody secretion of human Epstein Barr virus-activated B-lymphocytes with hybridoma formation by electrofusion. (nih.gov)
Cell fusion to produce hybridomas: The washed lymphocytes are mixed with myeloma cells in an appropriate medium which then exposed to PEG (polyethylene glycol) for a period of time and fusion takes place (Biology Discussion, 2018). (cainscrossing.org)

Hybridoma - A cell hybrid resulting from the fusion of a cancer cell and a normal lymphocyte (a type of white blood cell). (academic.ru)
Development of tumor B-cell lymphocyte hybridoma (TBH) autovaccination. (nih.gov)
To improve patient immune recognition of autologous tumor cells, we have developed a tumor B-cell lymphocyte hybridoma (TBH) autovaccination protocol. (nih.gov)
In 1975, Kohler and Milstein gave a concept of "one lymphocyte-one antibody" in the form of hybridoma (Kumar et al. (cainscrossing.org)
The fundamental of hybridoma technology is based on the immortalization of B lymphocyte cells having antibody producing capacity but limited growth characteristics in vitro. (cainscrossing.org)

To facilitate the analysis of the functional activities of this T-cell subpopulation, we developed a panel of 10 murine T-cell hybridomas from splenocytes of immune C57BL/6 mice. (asm.org)
We have previously reported that activation of murine T cell hybridomas leads to expression of Fas (CD95) and its ligand (FasL) which subsequently interact, even on the same cell, leading to apoptotic cell death. (uni-konstanz.de)

The SeqPack™ Hybridoma Preservation Kit is particularly useful for sequencing contaminated hybridoma cell lines, as it preserves any intact cells and prevents the infection from spreading. (nepic.co.uk)

By careful screening and mutation, a human-murine hybridoma suitable as a fusion partner for immortalizing an antibody-secreting B cell has been generated. (freepatentsonline.com)
Hybridoma technology includes cell fusion, the plating of master cell cultures, and antibody screening. (moleculardevices.com)
Third, hybridoma generation and screening. (digitaljournal.com)
Syngene also offers services surrounding Hybridoma generation and screening. (syngeneintl.com)
After the fusion is performed, the Hybridoma Center will notify the investigator when to expect supernatants for screening. (wustl.edu)
ELISA screening may also be contracted to the Hybridoma Center. (wustl.edu)
In cases where the screen for positive hybridomas requires special expertise, someone from the PI's lab participates in the initial screening. (yu.edu)

I am trying to CD8 T cells from OT-I transgenic mouse (using T cell proliferation assay)and B3Z T-T hybridoma (measure IL-2 activation), both of which recognize the same epitope SIINFEKL on Kb molecules. (bio.net)
MAbs were detected in serum and peritoneal lavage samples of mice bearing hybridoma tumors by an enzyme-linked immunosorbent assay and an immunoblot assay. (asm.org)
To assay the ability of the anti-H-2Kb alloreactive T cells to recognize the H-2Kb produced by Drosophila melanogaster cells, T cell hybridomas were produced. (nih.gov)
Upon engagement of the TCR, hybridomas prepared using this fusion partner produce β-galactosidase which can be detected by a colormatic assay. (nih.gov)

These MAbs reacted with viral antigens in immunofluorescence (IFA) and immunoperoxidase (IPX) in dilutions up to 1:640 (hybridoma supernatants) and 1:20.000 (ascitis fluid). (worldwidescience.org)
Thanks to our advanced hybridoma technology , our company has the ability to provide the one-stop custom production of mAbs. (digitaljournal.com)
Nevertheless, the potential of mAbs derived specifically from technology based on human hybridomas remains largely unfulfilled. (asmscience.org)
The principal reason for the lack of a large number of hybridoma-derived mAb therapeutics has simply been the technical difficulty in generating stable hybridomas that secrete human mAbs of high affinity and functional activity. (asmscience.org)
This chapter reviews recent efforts to develop and employ novel methods for the efficient generation of human hybridomas secreting human mAbs for clinical use. (asmscience.org)

Monoclonal Anti-Human CD4 (mouse IgG1 isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cell line NS-1 and splenocytes from Balb/c mice immunized with CD4-Transfected mouse T-cell hybridoma, 3DT, followed by CD4 + human T-cell CEM cells. (sigmaaldrich.com)
Recent isolates of RX54-3 hybridoma cells (new cells) protect BALB/c mice against subsequent challenge with the tumorigenic myeloma parent cells used to construct this hybridoma. (aacrjournals.org)
Cytomedical therapy for IgG1 plasmacytosis in human interleukin-6 transgenic mice using hybridoma cells microencapsulated in alginate-poly(L)lysine-alginate membrane. (nii.ac.jp)
Immunized spleen cells derived from either mice or Armenian hamsters are fused and maintained at the Hybridoma Center. (wustl.edu)
gammadelta T cell hybridomas derived from mice infected with Listeria were preferentially stimulated by low density macrophages from peritoneal exudates of infected mice. (nih.gov)
Stimulation of the γδ T cell hybridoma BC10 by PEC fractions from day 6 Listeria-infected mice. (nih.gov)
C) Flow cytometric analysis of BC10 hybridomas after culture with PECs from day 3 or 9 Listeria-infected wild-type mice. (nih.gov)
Highly significant protection was observed in mice bearing MT1 hybridoma tumors against both intraperitoneal and intranasal challenge infections with homologous nontoxigenic P. multocida strains possessing MAb MT1-reacting epitopes, whereas the mice bearing MT4.1 hybridoma tumors were not protected. (asm.org)
The numbers of P. multocida organisms in the lungs of mice bearing MT1 hybridoma tumors were significantly less than those in lungs of mice bearing MT4.1 hybridoma tumors at 48 h postchallenge. (asm.org)
However, an enhanced phagocytosis by polymorphonuclear cells (PMNs) was observed in mice bearing MT1 hybridoma tumors. (asm.org)
P. multocida induced a more extensive and rapid influx of PMNs into the peritoneal cavity of mice bearing MT1 hybridoma tumors than of mice bearing MT4.1 hybridoma tumors. (asm.org)

B cell hybridomas were cultured on the chip and the secretion of immunoglobulins (antibody) specific to HEL was monitored in real-time and detected within only few minutes rather than after a 30-60 min incubation with standard ELISA experiments. (inserm.fr)

Mab Anti Islet 1, supplied by Developmental Studies Hybridoma Bank, used in various techniques. (bioz.com)

When such a single hybridoma cell forms a cell population, a specific antibody against an antigen epitope, also known as the mAb, can be prepared. (digitaljournal.com)

Some hybridomas in the ATCC collection are somatic cell hybrids. (atcc.org)

Effect of Oxygen on Antibody Production in Hybridoma Culture. (springer.com)
In comparative cell growth studies, Hybridoma TP4-3.1 (proprietary) cells growing in IMDM + 10% FBS were adapted to growth in other media through a minimum of 3 passages prior to evaluation of growth and monoclonal antibody production in batch culture. (thermofisher.com)
InVitria Reveals Technology to Optimize Hybridoma Production P... ( Article in S. (bio-medicine.org)
Also disclosed are methods of simulating hybridoma cells to increase monoclonal antibody production by administering a CD40 ligand polypeptide that stimulates B cell proliferation. (google.com.au)
Its service portfolio includes mouse and rat monoclonal antibody production using hybridoma technology, human, monkey, rabbit, chicken, dog, llama and camel monoclonal antibody production using various antibody library technologies. (digitaljournal.com)
In this report we describe a functional role for Shc in two events that occur during T cell activation, AICD and IL-2 production, in a T cell hybridoma line that has been previously shown to undergo apoptosis and produce IL-2 upon cross-linking of the TCR/CD3 complex. (jimmunol.org)
Monoclonal Antibody Technology: The Production and Characterization of Rodent and Human Hybridomas: The Production and Characterization of Rodent and Human Hybridomas Edition by Campbell, A.M. and Publisher Elsevier Science. (vitalsource.com)

Functional analysis of Histoplasma capsulatum-reactive T-cell hybridomas. (asm.org)
The role of class II MHC molecules in the activation of class I-reactive T cell hybridomas. (jimmunol.org)
To examine the role of Ia molecules in T cell responses to allo-class I major histocompatibility antigens, a series of allo-class I-reactive T cell hybridomas was established. (jimmunol.org)
Of 134 T cell hybridomas obtained from the fusion of C3H/HeJm or B10.HTT T cells stimulated with C57BL/6 splenocytes, nine T cell hybridomas were reactive to class I antigens and 126 T cell hybridomas were reactive to class II antigens. (jimmunol.org)

Covance has generated hybridomas against a large variety of antigens. (bio-medicine.org)
Of 10 hybridomas studied, only one recognized heterologous fungal antigens. (asm.org)
These hybridomas provide a useful tool for defining yeast-phase antigens that trigger T-cell activation. (asm.org)
Three of these T cell hybridomas, HTB-157.7, HTB-176.10, and HTB-177.2, could react to the EL-4 cell line that expresses H-2Kb and H-2Db class I antigens but lacks class II I-Ab molecules. (jimmunol.org)

XP Media and CloneMedia for Mouse Hybridoma Generation provides a complete solution that supports all stages of hybridoma cell line development. (moleculardevices.com)

Search a wide selection of hybridomas alphanumerically. (atcc.org)

Selected subclones were further tested for their ability to produce a stable hybridoma and monoclonal antibody secretion. (wikipedia.org)

Since the immunosuppressive drugs cyclosporin A (CsA) and FK506 block activation-induced apoptosis in T cell hybridomas, we examined whether such compounds affect cell death by interfering with expression of Fas, FasL or both, or whether they block Fas signal transduction. (uni-konstanz.de)
We therefore conclude that CsA and FK506 block activation-induced apoptosis in T cell hybridomas predominantly by interfering with activation signals leading to FasL expression and, further, that the regulation of the expression of Fas and FasL on activated T cells is differentially controlled. (uni-konstanz.de)

After immunization, B-cells are fused with a candidate rabbit fusion partner cell line to form hybridomas. (wikipedia.org)
This approach is based on immunization of a cancer patient with a hybridoma cell suspension derived from the fusion of autologous activated B-cells and autologous cancer cells. (nih.gov)

PEG-4000 is used to assist in the fusion of myeloma and spleen cells to form hybridomas. (atcc.org)
A rabbit hybridoma is a hybrid cell line formed by the fusion of an antibody producing rabbit B cell with a cancerous B-cell (myeloma). (wikipedia.org)
I understand that the fusion rate (# hybridomas obtained after fusion) is very low (5-10? (protocol-online.org)
The fusion product is called a hybridoma cell. (sciencephoto.com)
ClonaCell™-HY media are specifically designed to be a complete solution for hybridoma generation, from cell fusion to hybridoma expansion. (stemcell.com)
Perform fusion of myeloma cells and splenocytes to yield between 10 - 80 million fused hybridoma cells. (stemcell.com)
Collagen type II (C-II)-specific T cell hybridomas are transduced with a lentivirus carrying a triple fusion reporter gene (TFR) construct consisting of a fluorescent reporter gene (RG), a bioluminescent RG (hRluc), and a positron emission tomography (PET) RG. (spiedigitallibrary.org)
We are stll trying to establish the hybridomas using other techniques such as an electroric fusion. (nii.ac.jp)
On the scheduled fusion day, the investigator will bring the host animal to the Hybridoma Center - West Building, Room 4724. (wustl.edu)
If the investigator is late, the fusion may be cancelled at the discretion of the Hybridoma Center. (wustl.edu)
Because of variable loss of chromosomes, cloned hybridoma cells from one fusion exhibit different fingerprint patterns. (iospress.com)
Thus, antibody-secreting B cell hybridomas can be distinguished easily even when they originate from the same fusion. (iospress.com)
In this technology, immortal hybridomas which are cell fusion of B-cells and myeloma cells are grown on artificial medium. (cainscrossing.org)

In the current work we describe the isolation and characterization of a hybridoma secreting a high-affinity, NNK-specific monoclonal antibody. (mdpi.com)

The hybridoma culture supernatant, secondary enzyme labeled conjugate, and chromogenic substrate, are then incubated, and the formation of a colored product indicates a positive hybridoma. (wikipedia.org)

False-colour scanning electron micrograph of a hybridoma cell producing a monoclonal antibody to cytoskeleton protein. (sciencephoto.com)

High-throughput next-generation (NGS) sequencing is used to deliver all variable domains within a hybridoma, a process that works on any species or isotype and can often rescue contaminated or unproductive hybridoma cell lines. (nepic.co.uk)

The Washington University School of Medicine Hybridoma Center is under the faculty supervision of Kathleen Sheehan, PhD and is operated by Research Technicians Rebecca Andrews and Chelsea Mannie. (wustl.edu)

Search for hybridomas by mouse, lymphoblast or somatic cell used to develop the line. (atcc.org)
These antibody producing B-cells are then harvested from the mouse and, in turn, fused with immortal B cell cancer cells, a myeloma,[clarification needed] to produce a hybrid cell line called a hybridoma, which has both the antibody-producing ability of the B-cell and the longevity and reproductivity of the myeloma. (wikipedia.org)
Hybridoma -tested serum has been shown to support mouse myeloma cells and hybridoma cells in culture. (sigmaaldrich.com)
CD4-transfected mouse T-cell hybridoma, 3DT, followed by CD4+ human T-cell CEM cells. (sigmaaldrich.com)
We have transfected the mouse CD4 gene into a beef insulin (BI)-specific murine T helper hybridoma that lacks CD4 surface expression. (rupress.org)
A hybridoma is an immortalised mouse cell line that produces a particular monoclonal antibody. (blogspot.com)
Analysis of the interaction between an alpha (1----6)dextran-specific mouse hybridoma antibody and dextran B512 by affinity electrophoresis. (biomedsearch.com)
The mouse or Armenian hamster host to be fused may be obtained, immunized, boosted and bled by the investigator or can be contracted through the Hybridoma Center. (wustl.edu)
Equivalence of human and mouse CD4 in enhancing antigen responses by a mouse class II-restricted T cell hybridoma. (rupress.org)
We have examined the ability of hCD4 to interact functionally with mouse class II MHC molecules using the mouse T cell hybridoma BI-141, specific for beef insulin. (rupress.org)
We now demonstrate that expression of hCD4 results in an equivalent stimulation of antigen responses by this mouse T cell hybridoma. (rupress.org)
HybridomaPrime is a serum-free medium, optimized to support growth of human, mouse and rat hybridoma as well as myeloma cells. (capricorn-scientific.com)
Hybrid cells propagate by employing Hybridoma technology that may be cultured in a laboratory or subcultured in a mouse bodily cavity. (espritrock.org)

This monoclonal antibody is produced by using a technology called "Hybridoma technology" which was discovered by George Kohler of West Germany and Cesal Milstein of Argentina in 1975 (Global-research-online.net, 2018). (cainscrossing.org)
2018). The Immune System Hybridoma Technology Biochem t. online Available at: http://imgdealix.pw/The-Immune-System-Hybridoma-Technology-Biochem-t.html Accessed 4 Nov. 2018. (cainscrossing.org)

Fort Collins, CO (PRWEB) April 22, 2009-- Recent studies using both classical media and the leading commercial serum-free hybridoma media formulations proved that InVitria's cell culture supplements optimized cell growth and productivity of hybridoma cells.1 Further, the studies revealed that Cellastim supports cell adaptation and survival in chemically-defined media. (bio-medicine.org)
The hybridoma line, growth medium and the quantitation of human IgG were provided by Medarex (Milpitas, CA). The ImageXpress Velos System was configured with a 488 nm laser. (moleculardevices.com)
A protein-free medium for the growth of hybridomas and other cells of the immune system. (nih.gov)
ABC supports the long-term growth of murine hybridomas as well as other transformed cells of the immune system. (nih.gov)
The requirement of hybridoma growth for transferrin has been met by substituting the soluble organo-iron compound, sodium nitroprusside. (nih.gov)
Substantial improvement in the growth of hybridomas was afforded by the inclusion of 18 trace elements complexed to disodium ethylene diaminetetraacetate (EDTA). (nih.gov)
Hybridoma Qualified lots must show superior growth promotion, exceptional clonal growth at limiting dilution, and a lack of cytotoxicity during the growth studies. (rndsystems.com)
Each batch of BriClone is tested for its ability to support the outgrowth of hybridomas from freshly fused cells and for its ability to support the growth of hybridomas under conditions of limiting dilution. (qedbio.com)
Interleukin-HP1, a T cell-derived hybridoma growth factor that supports the in vitro growth of murine plasmacytomas. (rupress.org)
We have recently described the purification and NH2-terminal amino acid sequence of a T cell-derived hybridoma growth factor that was provisionally designated interleukin-HP1 (IL-HP1). (rupress.org)
Here we report that a T cell supernatant containing high titers of this hybridoma growth factor considerably facilitated the establishment of primary cultures of murine plasmacytomas. (rupress.org)
This conclusion was further supported by the finding that IL-HP1 purified to homogeneity supported the growth of both B cell hybridomas and plasmacytomas. (rupress.org)
For half-maximal growth, plasmacytomas, however, required a concentration of IL-HP1 of approximately 30 pM, which is approximately 200 times higher than that required by B cell hybridomas. (rupress.org)

the resulting hybridoma cells will produce the same secretion as the normal parent cells and proliferate indefinitely in culture like the parent tumor cells. (academic.ru)

A murine hybridoma cell line has been established that consistently forms large cytoplasmic inclusions. (rupress.org)
In contrast, the other three T cell hybridomas, CB-127.6, CB-221.7, and HTB-102.7, failed to react with EL-4 but reacted with the LB cell line which expresses class I (H-2Kb, H-2Db) and class II (I-Ab) molecules. (jimmunol.org)
Hybridoma cell line 500 expresses monoclonal antibody specific for Moloney murine leukemia virus (MuLV) gp80. (nih.gov)

Within 3 weeks, the reactivity of hybridomas to histoplasmin declined dramatically, yet the cells responded vigorously to yeast-phase preparations that were enriched for cytosol, cell wall, or cell membrane. (asm.org)
A) Reactivity of alloreactive anti H-2Kb hybridoma 34. (nih.gov)
A) Reactivity of alloreactive anti H-2Kb hybridoma 34.9 with non-transfected T2 and T2 cells transfected with H-2Kb. (nih.gov)
B) Reactivity of hybridoma 34.9 to immobilized soluble H-2Kb treated with either PBS at pH 7.4 (middle) or 100 μM sodium acetate/acetic acid buffer at pH 3.0 (right). (nih.gov)

The resulting hybrid cells, called hybridoma s, combine the longevity of the myeloma cell with the ability to produce a specific antibody and so are able to produce potentially unlimited amounts of the desired antibody. (britannica.com)
Each hybridoma produces a unique, sequence specific antibody. (blogspot.com)
The novelty of the claims thus depended on whether the claims could be said to be limited to the specific antibody sequences produced by the hybridomas. (blogspot.com)

Conventional methods to select and clone antibody-producing hybridomas involve multiple dilution steps in liquid medium. (stemcell.com)
Hybridoma cell lines EP-16 and 7P41A were cloned by limiting dilution under conditions designed to seed 1 cell/well in 96-well plates. (qedbio.com)

Shc is rapidly phosphorylated on tyrosine residues in response to TCR engagement, and phosphorylated Shc subsequently binds to Grb2 and mSos, two proteins involved in Ras activation, in both T cell hybridomas and normal human PBL ( 4 ). (jimmunol.org)
Some component in the rat hybridoma supernatant influenced gamma retrovirus infection, either with the cellular Pit receptor or with the envelope proteins, when added to human TE cells and gamma retrovirus. (biology-online.org)

Through these initiatives, the Hybridoma Core will remain on the cutting edge of monoclonal antibody technology, and continue to provide optimal service to investigators. (umich.edu)
CD Hybridoma Medium is available in a ready-to-use (1X) liquid format and in our easy-to-use Advanced Granulation Technology™ (AGT™) format. (thermofisher.com)
The Washington University School of Medicine Hybridoma Center (WUHC) provides monoclonal antibody technology to the University research community. (wustl.edu)
These hybrid cells produced monoclonal antibody and this process is point out as Hybridoma technology. (espritrock.org)
In the course of making the hybridomas, students and fellows can receive training on how to carry out the hybridoma technology. (yu.edu)

Because it is manufactured with animal origin-free materials, protein purification and downstream processing of cultures grown in CD Hybridoma Medium is streamlined compared to that required for cultures grown in less defined media. (thermofisher.com)

50 ml Serum-free Hybridoma Supplement (10x) Cat. (capricorn-scientific.com)

The transfected clone showed a 50-fold increased sensitivity towards BI in comparison to the same CD4- hybridoma. (rupress.org)

Furthermore, the activation of these three T cell hybridomas with C57BL/6-derived splenocytes was not blocked by either anti-I-A or anti-L3T4 antibody. (jimmunol.org)

On chip real time monitoring of B-cells hybridoma secretion of immunoglobulin. (inserm.fr)

Anatomy8

Organisms7

Diseases2

Chemicals and Drugs39

Analytical, Diagnostic and Therapeutic Techniques and Equipment5

Phenomena and Processes21

Information Science3

Health Care1

Repertoire of human antibodies against the polysaccharide capsule of Streptococcus pneumoniae serotype 6B. (1/2670)

Mechanisms of double-strand-break repair during gene targeting in mammalian cells. (2/2670)

The molecular basis of multiple vector insertion by gene targeting in mammalian cells. (3/2670)

Infrequent translation of a nonsense codon is sufficient to decrease mRNA level. (4/2670)

Soluble class I MHC with beta2-microglobulin covalently linked peptides: specific binding to a T cell hybridoma. (5/2670)

Cloning, expression, and characterization of the Fab fragment of the anti-lysozyme antibody HyHEL-5. (6/2670)

A novel 62-kilodalton egg antigen from Schistosoma mansoni induces a potent CD4(+) T helper cell response in the C57BL/6 mouse. (7/2670)

Immunosuppressant deoxyspergualin-induced inhibition of cell proliferation is accompanied with an enhanced reduction of tetrazolium salt. (8/2670)

Hybridomas

Antibodies, Monoclonal

Antibody Specificity

Epitopes

Mice, Inbred BALB C

T-Lymphocytes

Immunoglobulin Variable Region

Immunoglobulin Heavy Chains

B-Lymphocytes

Histocompatibility Antigens Class II

Plasmacytoma

Cell Fusion

Hybrid Cells

Binding Sites, Antibody

Cross Reactions

Immunoglobulin Light Chains

Immunoglobulin Idiotypes

Genes, Immunoglobulin

Immunoglobulin kappa-Chains

Lymphocyte Activation

Receptors, Antigen, T-Cell

Clone Cells

Antigens, Surface

Suppressor Factors, Immunologic

Mice, Inbred Strains

Enzyme-Linked Immunosorbent Assay

Molecular Sequence Data

Cell Line

Spleen

Interleukin-2

Immunoglobulin M

Immunoglobulin Allotypes

Amino Acid Sequence

Lymphocyte Cooperation

Mice, Inbred CBA

Antigen-Antibody Reactions

Histoplasmin

Immunoglobulin G

Antigens

Immunoglobulin mu-Chains

Antigen-Antibody Complex

Antigen-Presenting Cells

Major Histocompatibility Complex

p-Azobenzenearsonate

Antibody Affinity

H-2 Antigens

Genes, MHC Class II

Antibody Diversity

Autoantibodies

Receptors, Antigen, T-Cell, alpha-beta

Base Sequence

Mice, Inbred C57BL

Phenylacetates

Fluorescent Antibody Technique

Antigen Presentation

Thymoma

Mice, Inbred A

Prostatic Secretory Proteins

Immunoglobulin lambda-Chains

Trinitrobenzenes

Mice, Inbred NZB

Gene Rearrangement, B-Lymphocyte, Light Chain

Mice, Inbred AKR

Antibody Formation

Immunization

Lymphokines

Immunoglobulin Isotypes

Antibodies, Antinuclear

Antibodies, Anti-Idiotypic

Antigens, CD5

Myeloma Proteins

Antibodies, Heterophile

Autoantigens

Immunoglobulins

Muramidase

Antigens, Neoplasm

Antigens, Viral

Gene Rearrangement, B-Lymphocyte

Repertoire of human antibodies against the polysaccharide capsule of Streptococcus pneumoniae serotype 6B. (1/2670)

Mechanisms of double-strand-break repair during gene targeting in mammalian cells. (2/2670)