Hybridomas
Antibody Specificity
T-Lymphocytes
Lymphocytes responsible for cell-mediated immunity. Two types have been identified - cytotoxic (T-LYMPHOCYTES, CYTOTOXIC) and helper T-lymphocytes (T-LYMPHOCYTES, HELPER-INDUCER). They are formed when lymphocytes circulate through the THYMUS GLAND and differentiate to thymocytes. When exposed to an antigen, they divide rapidly and produce large numbers of new T cells sensitized to that antigen.
Immunoglobulin Variable Region
That region of the immunoglobulin molecule that varies in its amino acid sequence and composition, and comprises the binding site for a specific antigen. It is located at the N-terminus of the Fab fragment of the immunoglobulin. It includes hypervariable regions (COMPLEMENTARITY DETERMINING REGIONS) and framework regions.
Immunoglobulin Heavy Chains
B-Lymphocytes
Histocompatibility Antigens Class II
Large, transmembrane, non-covalently linked glycoproteins (alpha and beta). Both chains can be polymorphic although there is more structural variation in the beta chains. The class II antigens in humans are called HLA-D ANTIGENS and are coded by a gene on chromosome 6. In mice, two genes named IA and IE on chromosome 17 code for the H-2 antigens. The antigens are found on B-lymphocytes, macrophages, epidermal cells, and sperm and are thought to mediate the competence of and cellular cooperation in the immune response. The term IA antigens used to refer only to the proteins encoded by the IA genes in the mouse, but is now used as a generic term for any class II histocompatibility antigen.
Plasmacytoma
Cell Fusion
Hybrid Cells
Binding Sites, Antibody
Cross Reactions
Immunoglobulin Light Chains
Immunoglobulin Idiotypes
Unique genetically-controlled determinants present on ANTIBODIES whose specificity is limited to a single group of proteins (e.g., another antibody molecule or an individual myeloma protein). The idiotype appears to represent the antigenicity of the antigen-binding site of the antibody and to be genetically codetermined with it. The idiotypic determinants have been precisely located to the IMMUNOGLOBULIN VARIABLE REGION of both immunoglobin polypeptide chains.
Genes, Immunoglobulin
Genes encoding the different subunits of the IMMUNOGLOBULINS, for example the IMMUNOGLOBULIN LIGHT CHAIN GENES and the IMMUNOGLOBULIN HEAVY CHAIN GENES. The heavy and light immunoglobulin genes are present as gene segments in the germline cells. The completed genes are created when the segments are shuffled and assembled (B-LYMPHOCYTE GENE REARRANGEMENT) during B-LYMPHOCYTE maturation. The gene segments of the human light and heavy chain germline genes are symbolized V (variable), J (joining) and C (constant). The heavy chain germline genes have an additional segment D (diversity).
Immunoglobulin kappa-Chains
Lymphocyte Activation
Morphologic alteration of small B LYMPHOCYTES or T LYMPHOCYTES in culture into large blast-like cells able to synthesize DNA and RNA and to divide mitotically. It is induced by INTERLEUKINS; MITOGENS such as PHYTOHEMAGGLUTININS, and by specific ANTIGENS. It may also occur in vivo as in GRAFT REJECTION.
Receptors, Antigen, T-Cell
Molecules on the surface of T-lymphocytes that recognize and combine with antigens. The receptors are non-covalently associated with a complex of several polypeptides collectively called CD3 antigens (ANTIGENS, CD3). Recognition of foreign antigen and the major histocompatibility complex is accomplished by a single heterodimeric antigen-receptor structure, composed of either alpha-beta (RECEPTORS, ANTIGEN, T-CELL, ALPHA-BETA) or gamma-delta (RECEPTORS, ANTIGEN, T-CELL, GAMMA-DELTA) chains.
Clone Cells
A group of genetically identical cells all descended from a single common ancestral cell by mitosis in eukaryotes or by binary fission in prokaryotes. Clone cells also include populations of recombinant DNA molecules all carrying the same inserted sequence. (From King & Stansfield, Dictionary of Genetics, 4th ed)
Antigens, Surface
Suppressor Factors, Immunologic
Proteins, protein complexes, or glycoproteins secreted by suppressor T-cells that inhibit either subsequent T-cells, B-cells, or other immunologic phenomena. Some of these factors have both histocompatibility (I-J) and antigen-specific domains which may be linked by disulfide bridges. They can be elicited by haptens or other antigens and may be mass-produced by hybridomas or monoclones in the laboratory.
Mice, Inbred Strains
Genetically identical individuals developed from brother and sister matings which have been carried out for twenty or more generations, or by parent x offspring matings carried out with certain restrictions. All animals within an inbred strain trace back to a common ancestor in the twentieth generation.
Enzyme-Linked Immunosorbent Assay
An immunoassay utilizing an antibody labeled with an enzyme marker such as horseradish peroxidase. While either the enzyme or the antibody is bound to an immunosorbent substrate, they both retain their biologic activity; the change in enzyme activity as a result of the enzyme-antibody-antigen reaction is proportional to the concentration of the antigen and can be measured spectrophotometrically or with the naked eye. Many variations of the method have been developed.
Molecular Sequence Data
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
Interleukin-2
Immunoglobulin M
Immunoglobulin Allotypes
Amino Acid Sequence
Antigen-Antibody Reactions
Immunoglobulin G
Immunoglobulin mu-Chains
Antigen-Antibody Complex
Antigen-Presenting Cells
A heterogeneous group of immunocompetent cells that mediate the cellular immune response by processing and presenting antigens to the T-cells. Traditional antigen-presenting cells include MACROPHAGES; DENDRITIC CELLS; LANGERHANS CELLS; and B-LYMPHOCYTES. FOLLICULAR DENDRITIC CELLS are not traditional antigen-presenting cells, but because they hold antigen on their cell surface in the form of IMMUNE COMPLEXES for B-cell recognition they are considered so by some authors.
Major Histocompatibility Complex
The genetic region which contains the loci of genes which determine the structure of the serologically defined (SD) and lymphocyte-defined (LD) TRANSPLANTATION ANTIGENS, genes which control the structure of the IMMUNE RESPONSE-ASSOCIATED ANTIGENS, HUMAN; the IMMUNE RESPONSE GENES which control the ability of an animal to respond immunologically to antigenic stimuli, and genes which determine the structure and/or level of the first four components of complement.
p-Azobenzenearsonate
Antibody Affinity
A measure of the binding strength between antibody and a simple hapten or antigen determinant. It depends on the closeness of stereochemical fit between antibody combining sites and antigen determinants, on the size of the area of contact between them, and on the distribution of charged and hydrophobic groups. It includes the concept of "avidity," which refers to the strength of the antigen-antibody bond after formation of reversible complexes.
Genes, MHC Class II
Antibody Diversity
The phenomenon of immense variability characteristic of ANTIBODIES. It enables the IMMUNE SYSTEM to react specifically against the essentially unlimited kinds of ANTIGENS it encounters. Antibody diversity is accounted for by three main theories: (1) the Germ Line Theory, which holds that each antibody-producing cell has genes coding for all possible antibody specificities, but expresses only the one stimulated by antigen; (2) the Somatic Mutation Theory, which holds that antibody-producing cells contain only a few genes, which produce antibody diversity by mutation; and (3) the Gene Rearrangement Theory, which holds that antibody diversity is generated by the rearrangement of IMMUNOGLOBULIN VARIABLE REGION gene segments during the differentiation of the ANTIBODY-PRODUCING CELLS.
Autoantibodies
Receptors, Antigen, T-Cell, alpha-beta
Base Sequence
Phenylacetates
Derivatives of phenylacetic acid. Included under this heading are a variety of acid forms, salts, esters, and amides that contain the benzeneacetic acid structure. Note that this class of compounds should not be confused with derivatives of phenyl acetate, which contain the PHENOL ester of ACETIC ACID.
Fluorescent Antibody Technique
Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
Antigen Presentation
The process by which antigen is presented to lymphocytes in a form they can recognize. This is performed by antigen presenting cells (APCs). Some antigens require processing before they can be recognized. Antigen processing consists of ingestion and partial digestion of the antigen by the APC, followed by presentation of fragments on the cell surface. (From Rosen et al., Dictionary of Immunology, 1989)
Thymoma
A neoplasm originating from thymic tissue, usually benign, and frequently encapsulated. Although it is occasionally invasive, metastases are extremely rare. It consists of any type of thymic epithelial cell as well as lymphocytes that are usually abundant. Malignant lymphomas that involve the thymus, e.g., lymphosarcoma, Hodgkin's disease (previously termed granulomatous thymoma), should not be regarded as thymoma. (From Stedman, 25th ed)
Prostatic Secretory Proteins
Immunoglobulin lambda-Chains
Gene Rearrangement, B-Lymphocyte, Light Chain
Antibody Formation
Immunization
Deliberate stimulation of the host's immune response. ACTIVE IMMUNIZATION involves administration of ANTIGENS or IMMUNOLOGIC ADJUVANTS. PASSIVE IMMUNIZATION involves administration of IMMUNE SERA or LYMPHOCYTES or their extracts (e.g., transfer factor, immune RNA) or transplantation of immunocompetent cell producing tissue (thymus or bone marrow).
Lymphokines
Immunoglobulin Isotypes
Antibodies, Antinuclear
Autoantibodies directed against various nuclear antigens including DNA, RNA, histones, acidic nuclear proteins, or complexes of these molecular elements. Antinuclear antibodies are found in systemic autoimmune diseases including systemic lupus erythematosus, Sjogren's syndrome, scleroderma, polymyositis, and mixed connective tissue disease.
Antibodies, Anti-Idiotypic
Antigens, CD5
Glycoproteins expressed on all mature T-cells, thymocytes, and a subset of mature B-cells. Antibodies specific for CD5 can enhance T-cell receptor-mediated T-cell activation. The B-cell-specific molecule CD72 is a natural ligand for CD5. (From Abbas et al., Cellular and Molecular Immunology, 2d ed, p156)
Antibodies, Heterophile
Antibodies elicited in a different species from which the antigen originated. These antibodies are directed against a wide variety of interspecies-specific antigens, the best known of which are Forssman, Hanganutziu-Deicher (H-D), and Paul-Bunnell (P-B). Incidence of antibodies to these antigens--i.e., the phenomenon of heterophile antibody response--is useful in the serodiagnosis, pathogenesis, and prognosis of infection and latent infectious states as well as in cancer classification.
Autoantigens
Immunoglobulins
Multi-subunit proteins which function in IMMUNITY. They are produced by B LYMPHOCYTES from the IMMUNOGLOBULIN GENES. They are comprised of two heavy (IMMUNOGLOBULIN HEAVY CHAINS) and two light chains (IMMUNOGLOBULIN LIGHT CHAINS) with additional ancillary polypeptide chains depending on their isoforms. The variety of isoforms include monomeric or polymeric forms, and transmembrane forms (B-CELL ANTIGEN RECEPTORS) or secreted forms (ANTIBODIES). They are divided by the amino acid sequence of their heavy chains into five classes (IMMUNOGLOBULIN A; IMMUNOGLOBULIN D; IMMUNOGLOBULIN E; IMMUNOGLOBULIN G; IMMUNOGLOBULIN M) and various subclasses.
Muramidase
A basic enzyme that is present in saliva, tears, egg white, and many animal fluids. It functions as an antibacterial agent. The enzyme catalyzes the hydrolysis of 1,4-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrin. EC 3.2.1.17.
Antigens, Neoplasm
Repertoire of human antibodies against the polysaccharide capsule of Streptococcus pneumoniae serotype 6B. (1/2670)
We examined the repertoire of antibodies to Streptococcus pneumoniae 6B capsular polysaccharide induced with the conventional polysaccharide vaccine in adults at the molecular level two ways. In the first, we purified from the sera of seven vaccinees antipneumococcal antibodies and determined their amino acid sequences. Their VH regions are mainly the products of VH3 family genes (candidate genes, 3-23, 3-07, 3-66, and 3-74), but the product of a VH1 family gene (candidate gene, 1-03) is occasionally used. All seven individuals have small amounts of polyclonal kappa+ antibodies (Vkappa1 to Vkappa4 families), although kappa+ antibodies are occasionally dominated by antibodies formed with the product of the A27 Vkappa gene. In contrast, lambda+ anti-6B antibodies are dominated by the antibodies derived from one of 3 very similar Vlambda2 family genes (candidate genes, 2c, 2e, and 2a2) and Clambda1 gene product. The Vlambda2(+) antibodies express the 8.12 idiotype, which is expressed on anti-double-stranded-DNA antibodies. In one case, Vlambda is derived from a rarely expressed Vlambda gene, 10a. In the second approach, we studied a human hybridoma (Dob1) producing anti-6B antibody. Its VH region sequence is closely related to those of the 3-15 VH gene (88% nucleotide homology) and JH4 (92% homology). Its VL region is homologous to the 2a2 Vlambda2 gene (91%) and Jlambda1/Clambda1. Taken together, the V region of human anti-6B antibodies is commonly formed by a VH3 and a Vlambda2 family gene product. (+info)Mechanisms of double-strand-break repair during gene targeting in mammalian cells. (2/2670)
In the present study, the mechanism of double-strand-break (DSB) repair during gene targeting at the chromosomal immunoglobulin mu-locus in a murine hybridoma was examined. The gene-targeting assay utilized specially designed insertion vectors genetically marked in the region of homology to the chromosomal mu-locus by six diagnostic restriction enzyme site markers. The restriction enzyme markers permitted the contribution of vector-borne and chromosomal mu-sequences in the recombinant product to be determined. The use of the insertion vectors in conjunction with a plating procedure in which individual integrative homologous recombination events were retained for analysis revealed several important features about the mammalian DSB repair process:The presence of the markers within the region of shared homology did not affect the efficiency of gene targeting. In the majority of recombinants, the vector-borne marker proximal to the DSB was absent, being replaced with the corresponding chromosomal restriction enzyme site. This result is consistent with either formation and repair of a vector-borne gap or an "end" bias in mismatch repair of heteroduplex DNA (hDNA) that favored the chromosomal sequence. Formation of hDNA was frequently associated with gene targeting and, in most cases, began approximately 645 bp from the DSB and could encompass a distance of at least 1469 bp. The hDNA was efficiently repaired prior to DNA replication. The repair of adjacent mismatches in hDNA occurred predominantly on the same strand, suggesting the involvement of a long-patch repair mechanism. (+info)The molecular basis of multiple vector insertion by gene targeting in mammalian cells. (3/2670)
Gene targeting using sequence insertion vectors generally results in integration of one copy of the targeting vector generating a tandem duplication of the cognate chromosomal region of homology. However, occasionally the target locus is found to contain >1 copy of the integrated vector. The mechanism by which the latter recombinants arise is not known. In the present study, we investigated the molecular basis by which multiple vectors become integrated at the chromosomal immunoglobulin mu locus in a murine hybridoma. To accomplish this, specially designed insertion vectors were constructed that included six diagnostic restriction enzyme markers in the Cmu region of homology to the target chromosomal mu locus. This enabled contributions by the vector-borne and chromosomal Cmu sequences at the recombinant locus to be ascertained. Targeted recombinants were isolated and analyzed to determine the number of vector copies integrated at the chromosomal immunoglobulin mu locus. Targeted recombinants identified as bearing >1 copy of the integrated vector resulted from a Cmu triplication formed by two vector copies in tandem. Examination of the fate of the Cmu region markers suggested that this class of recombinant was generated predominantly, if not exclusively, by two targeted vector integration events, each involving insertion of a single copy of the vector. Both vector insertion events into the chromosomal mu locus were consistent with the double-strand-break repair mechanism of homologous recombination. We interpret our results, taken together, to mean that a proportion of recipient cells is in a predetermined state that is amenable to targeted but not random vector integration. (+info)Infrequent translation of a nonsense codon is sufficient to decrease mRNA level. (4/2670)
In many organisms nonsense mutations decrease the level of mRNA. In the case of mammalian cells, it is still controversial whether translation is required for this nonsense-mediated RNA decrease (NMD). Although previous analyzes have shown that conditions that impede translation termination at nonsense codons also prevent NMD, the residual level of termination was unknown in these experiments. Moreover, the conditions used to impede termination might also have interfered with NMD in other ways. Because of these uncertainties, we have tested the effects of limiting translation of a nonsense codon in a different way, using two mutations in the immunoglobulin mu heavy chain gene. For this purpose we exploited an exceptional nonsense mutation at codon 3, which efficiently terminates translation but nonetheless maintains a high level of mu mRNA. We have shown 1) that translation of Ter462 in the double mutant occurs at only approximately 4% the normal frequency, and 2) that Ter462 in cis with Ter3 can induce NMD. That is, translation of Ter462 at this low (4%) frequency is sufficient to induce NMD. (+info)Soluble class I MHC with beta2-microglobulin covalently linked peptides: specific binding to a T cell hybridoma. (5/2670)
Soluble forms of the mouse MHC class I molecule, Dd, were produced in which the peptide binding groove was uniformly occupied by peptides attached via a covalent flexible peptide linker to the N terminus of the associated beta2-microglobulin. The MHC heavy chain and beta2-microglobulin were firmly associated, and the molecules displayed an Ab epitope requiring proper occupancy of the peptide binding groove. Soluble Dd containing a covalent version of a well-characterized Dd-binding peptide from HIV stimulated a T cell hybridoma specific for this combination. Furthermore, a tetravalent version of this molecule bound specifically with apparent high avidity to this hybridoma. (+info)Cloning, expression, and characterization of the Fab fragment of the anti-lysozyme antibody HyHEL-5. (6/2670)
Hybridoma cDNAs encoding the individual chains of the Fab fragment of the well characterized murine monoclonal antibody HyHEL-5 were cloned and sequenced. The recombinant Fab fragment was produced by expressing each chain in a separate Escherichia coli pET vector, denaturing inclusion bodies and co-refolding. Characterization of the purified Fab by MALDI-TOF mass spectrometry and N-terminal amino acid sequencing demonstrated proper processing of the individual chains. The association of the recombinant Fab fragment with hen egg lysozyme and the avian epitope variant bobwhite quail lysozyme was found by isothermal titration calorimetry to have energetics very similar to that of the HyHEL-5 IgG. Heterologous expression of the HyHEL-5 Fab fragment opens the way to structure/function studies in this well-known system. (+info)A novel 62-kilodalton egg antigen from Schistosoma mansoni induces a potent CD4(+) T helper cell response in the C57BL/6 mouse. (7/2670)
In infection with Schistosoma mansoni, hepatic granuloma formation is mediated by CD4(+) T helper (Th) cells sensitized to schistosomal egg antigens. There is considerable variation among infected individuals with respect to both severity of disease and the T-cell response to egg antigens. In the BL/6 mouse, the egg granulomas are relatively small and the relevant sensitizing egg antigens are largely unknown. We investigated the CD4(+) Th cell response of infected BL/6 mice to egg antigens fractionated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and found a prominent lymphoproliferative response to be directed against a 62-kDa component. With the aid of a specific T-cell hybridoma, 4E6, the 62-kDa antigen was isolated; following partial digestion with endoproteinase Glu-C, an internal amino acid sequence was found to be identical with one present in the enzyme phosphoenolpyruvate carboxykinase (PEPCK) of the organisms Caenorhabditis elegans and Treponema pallidum and to differ by one residue from PEPCK of various other species. In CD4(+) Th cells from 7.5- 8.5-week-infected BL/6 mice, the purified 62-kDa molecule elicited a potent proliferative response which, based on cytokine analysis, was of a mixed Th-1 and Th-2 type. Our results reveal a novel egg antigen of particular prominence in the BL/6 mouse and suggest that the immune response in schistosomiasis is a product of sensitization to egg antigens that may vary considerably in immunogenicity from strain to strain. (+info)Immunosuppressant deoxyspergualin-induced inhibition of cell proliferation is accompanied with an enhanced reduction of tetrazolium salt. (8/2670)
Deoxyspergualin (DSG) has both antitumor and immunosuppressive activities. We explored the mechanism of DSG activities using an aqueous soluble analogue, methyldeoxyspergualin (MeDSG) for in vitro culture studies. It is known that DSG has inhibitory effects on cell proliferation, and we also observed that MeDSG inhibited [3H]-thymidine incorporation by rapidly dividing murine T cell hybridomas. However, when tetrazolium (MTT) colorimetric assay was adopted to evaluate its inhibitory effects on cell proliferation, MeDSG induced an enhanced MTT reduction. When we examined whether these results were applicable to the actively dividing cells of other origins than T cells, similar effects were seen with Raji cells, J774.1 cells and NIH3T3 cells. N-30, another analogue which was capable of suppressing anti-SRBC antibody production in vivo, also induced inhibition of cell growth and an enhanced MTT reduction. In contrast, the analogue which failed to prevent the antibody production, neither enhanced MTT reduction nor inhibited cell proliferation. Our results demonstrated that the ability to generate MTT formazan in dividing cells is a common property among, DSG analogue with the immunosuppressive and antiproliferative activities. (+info)
Use of I region-restricted, antigen-specific T cell hybridomas to produce idiotypically specific anti-receptor antibodies. |...
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Hybridoma technology
The hybridomas can be grown in culture, each culture starting with one viable hybridoma cell, producing cultures each of which ... Hybridomas at the US National Library of Medicine Medical Subject Headings (MeSH) "Hybridoma Technology". Understanding Cancer ... Once a hybridoma colony is established, it will continually grow in culture medium like RPMI-1640 (with antibiotics and fetal ... Hybridoma technology is a method for producing large numbers of identical antibodies (also called monoclonal antibodies). This ...
Rabbit hybridoma
A rabbit hybridoma is a hybrid cell line formed by the fusion of an antibody producing rabbit B cell with a cancerous B-cell ( ... "Recombinant human interleukin-6 enhances the immunoglobulin secretion of a rabbit-rabbit hybridoma". Hybridoma. 20 (3): 189-98 ... The process of hybridoma formation in a rabbit first entails obtaining B-cells from a rabbit that has been immunized. There are ... Resulting antibodies from hybridomas are screened for an antigen which meets criteria of interest by diagnostic tests such as ...
Developmental Studies Hybridoma Bank
7 hybridoma deposits) RIKEN Stephen T. Warren (1 hybridoma deposit) Emory University Zena Werb (1 hybridoma deposit) University ... Wieschaus deposited 7 hybridomas National Academy of Sciences Members who have deposited hybridomas Utpal Banerjee (1 hybridoma ... 1 hybridoma deposit) Harvard University Max Cooper (1 hybridoma deposit) Emory University Marilyn Gist Farquhar (1 hybridoma ... There are currently over 5000 hybridomas in the DSHB collection. The DSHB has obtained hybridomas from a variety of individuals ...
IGHD
Antibodies Hybridomas. 3 (2): 65-74. doi:10.3233/HAB-1992-3202. PMID 1633267. Roes J, Rajewsky K (1992). "Cell autonomous ...
LSP1
Hybridoma. 17 (6): 497-507. doi:10.1089/hyb.1998.17.497. PMID 9890705. Pulford K, Jones M, Banham AH, et al. (1999). " ...
Collagen hybridizing peptide
Hybridoma. 19 (5): 375-385. doi:10.1089/02724570050198893. ISSN 0272-457X. PMID 11128027. Li, Y.; Foss, C. A.; Summerfield, D. ...
ASPH
Hybridoma. 28 (4): 251-7. doi:10.1089/hyb.2009.0017. PMID 19663697. Palumbo KS, Wands JR, Safran H, King T, Carlson RI, de la ...
AMY1A
1986). "Pancreatic amylase expression in human pancreatic development". Hybridoma. 5 (2): 137-45. doi:10.1089/hyb.1986.5.137. ...
Heterophile antibody
Hybridoma. 11 (1): 33-9. doi:10.1089/hyb.1992.11.33. PMID 1737638. Bolstad N, Warren DJ, Nustad K (October 2013). "Heterophilic ...
ICOSLG
Hybridoma. 30 (4): 361-368. doi:10.1089/hyb.2011.0014. PMID 21851236. Khayyamian S, Hutloff A, Büchner K, Gräfe M, Henn V, ...
Anti-actin antibodies
Hybridoma. 17 (5): 431-5. doi:10.1089/hyb.1998.17.431. PMID 9873988. Enright, Helen (Oct 1995). "Paraneoplastic autoimmune ...
ZBTB33
Hybridoma. 20 (3): 159-66. doi:10.1089/027245701750293484. PMID 11461664. Yoon HG, Chan DW, Reynolds AB, Qin J, Wong J (Sep ...
Camptodactyly-arthropathy-coxa vara-pericarditis syndrome
Hybridoma. 20 (3): 149-57. doi:10.1089/027245701750293475. PMID 11461663. (Articles with short description, Short description ...
Monocyte
Hybridoma. 7 (6): 521-527. doi:10.1089/hyb.1988.7.521. PMID 2466760. Passlick, Bernward; Flieger, Dimitri; Ziegler-Heitbrock, H ...
Hedgehog signaling pathway
Hybridoma. 26 (4): 231-40. doi:10.1089/hyb.2007.0507. PMID 17725385. Stecca B, Mas C, Ruiz i Altaba A (May 2005). "Interference ...
ING1
Hybridoma and Hybridomics. 21 (1): 1-10. doi:10.1089/15368590252917584. PMID 11991811. Bromidge T, Lynas C (Jul 2002). " ...
Cell culture
The National Centre for Cell Science (NCCS), Pune, India; national repository for cell lines/hybridomas etc. Public Health ... to produce a hybridoma which has the antibody specificity of the primary lymphocyte and the immortality of the myeloma. ...
Bispecific monoclonal antibody
In 1975, the problem of producing pure antibodies was solved by the creation of hybridoma technology, and the new era of ... These BsAbs are often manufactured with the quadroma, or the hybrid hybridoma, method. However, the quadroma method relies on ... In 1983, Milstein and Cuello created hybrid-hybridoma (quadroma) technology. In 1988, the single-chain variable fragment (scFv ... S2CID 4161444.(subscription required) Milstein C, Cuello AC (October 1983). "Hybrid hybridomas and their use in ...
Juliet Daniel
1999) Hybridoma 18: 343-349. 35. Daniel JM and Reynolds AB. The catenin p120ctn interacts with Kaiso, a novel BTB/POZ domain ... 2001) Hybridoma 20: 159-166. 33. Thoreson MA, Anastasiadis PZ, Daniel JM, Ireton RC, Wheelock MJ, Johnson KR, Hummingbird DK ...
Myc-tag
"Source for 9E10 anti-myc hybridoma". 2009-09-22. (Biochemistry, Peptide sequences). ... is available from the non-commercial Developmental Studies Hybridoma Bank. Protein tag Polyhistidine-tag SpyTag Flag-tag Evan ...
HAT medium
This process is called hybridoma technology. Laboratory animals (e.g., mice) are first exposed to an antigen against which we ...
Organization and expression of immunoglobulin genes
"Organization and expression of immunoglobulin genes in fetal liver hybridomas". Proceedings of the National Academy of Sciences ...
Activation-induced cytidine deaminase
"Activation-induced cytidine deaminase turns on somatic hypermutation in hybridomas". Nature. 415 (6873): 802-6. doi:10.1038/ ...
Monoclonal antibody
The hybridomas can be grown indefinitely in a suitable cell culture medium. They can also be injected into mice (in the ... The medium must be enriched during in vitro selection to further favour hybridoma growth. This can be achieved by the use of a ... Rabbit B-cells can be used to form a rabbit hybridoma. Polyethylene glycol is used to fuse adjacent plasma membranes, but the ... Only fused hybrid cells referred to as hybridomas, are able to grow indefinitely in the medium because the spleen cell partner ...
Dilution cloning
"Cloning by Limiting Dilution of Hybridoma". (All articles with dead external links, Articles with dead external links from July ...
Leonard Herzenberg
During a sabbatical in the laboratory of Cesar Milstein between 1976 and 1977, Herzenberg coined the term hybridoma for hybrid ... "The hybridoma revolution: an offshoot of basic research". BioEssays. 21 (11): 966-973. doi:10.1002/(SICI)1521-1878(199911)21:11 ...
César Milstein
The term hybridoma was proposed by Len Herzenberg during a sabbatical in my laboratory in 1976/1977. At a high-table ... The term hybridoma was coined by Leonard Herzenberg during his sabbatical in Milstein's laboratory between 1976 and 1977. ... The development of the hybridoma technology coupled to advances in nucleic acid sequencing allowed Milstein to chart the ... Milstein, César (11 October 1999). "The hybridoma revolution: an offshoot of basic research". BioEssays. 21 (11): 966-973. doi: ...
Donanemab
Little M, Kipriyanov SM, Le Gall F, Moldenhauer G (August 2000). "Of mice and men: hybridoma and recombinant antibodies". ...
Cell fusion
For production of monoclonal antibodies by producing hybridoma. For production of Induced stem cells. To assess protein ...
Douglas R. Green
Shi, Y. F.; Sahai, B. M.; Green, D. R. (1989). "Cyclosporin a inhibits activation-induced cell death in T-cell hybridomas and ... "Activation-induced cell death in T cell hybridomas is due to apoptosis. Morphologic aspects and DNA fragmentation". Journal of ... "Role for c-myc in activation-induced apoptotic cell death in T cell hybridomas". Science. 257 (5067): 212-4. Bibcode:1992Sci... ...
Browsing by Subject "Hybridomas"
Hybridoma technology with special reference to parasitic diseases : a notebook of papers and techniques presented at two ... Properties of the monoclonal antibodies produced by hybridoma technology and their application to the study of diseases : ... 1-5 October l979 and Hybridoma Technology with Special Reference to Parasitic Diseases, held in Belo Horizonte, Brazil, 19-23 ...
Antibody Discovery via Hybridomas | Molecular Devices
Optimizing the antibody discovery workflow from fusion to clone selection can dramatically accelerate hybridoma screening and ... Hybridoma Workflow In this video, Justin Dranschak presents Molecular Devices solution for a hybridoma workflow. Watch this ... now called a hybridoma cell. Because every B-cell produces a unique antibody, single-cell cloning of hybridomas can be used to ... Hybridoma workflow. Step 1: Fusion The process of fusing B cells, expressing unique antibodies, with myeloma cells creating a ...
Contact - Antibody Hybridoma Core - Mayo Clinic Research
Anti-α-Tubulin antibody, Mouse monoclonal clone DM1A, purified from hybridoma cell culture | Sigma-Aldrich
TCR-mediated adhesion of T cell hybridomas to planar bilayers containing purified MHC class II/peptide complexes and receptor...
A novel selection strategy for antibody producing hybridoma cells based on a new transgenic fusion cell line | Scientific...
The stably transfected myeloma cell line was used for the generation of hybridoma cells and an antigen- and isotype-specific ... based on an artificial cell surface construct by which secreted antibodies were connected to the corresponding hybridoma cell ... Isotype- and antigen-specific sorting of HA-AP-EGF-R+ hybridoma cells. Ten days after fusion HA-AP-EGF-R+ hybridoma cells were ... Staining of HA-AP-EGF-R bearing hybridoma cells. HA-AP-EGF-R bearing hybridoma cells were stained for receptor expression using ...
Antibodies & Hybridomas - page 1 of 12
Why Use Semi-Solid Medium for Cloning Hybridomas and CHO Cells | STEMCELL Technologies
Cloning hybridomas and CHO cells can be done faster and more efficiently using semi-solid cloning, compared to traditional ... Why Use Semi-Solid Medium for Cloning Hybridomas and CHO Cells Cloning hybridomas and CHO cells can be done faster and more ... Cloning hybridomas and CHO cells can be done faster and more efficiently using semi-solid cloning. During semi-solid cloning, ... Semi-solid methylcellulose-based medium for hybridoma selection and cloning, without HAT (serum-containing) ...
Hybridoma (Larchmt) | [email protected]
CD3 Antibody, anti-human | Hybridomas | MACS Antibodies | Products | Miltenyi Biotec | España
The CD3 antibody recognizes the human CD3 antigen which is present on mature human T cells, thymocytes, and NKT cells. The epitope recognized by the antibody is located on the ε-chain of the CD3 complex. Clone OKT3 has been shown to activate T cell function.Activation and proliferation of T cells can be induced by interaction of the T cell receptor with peptide-MHC complexes expressed on the surface of antigen-presenting cells (APCs). The T cell receives a signal transduced through the CD3 complex. Cytokines or other costimulatory signals are required in addition. Activated T cells can be used for any downstream processes, such as cytokine analysis or immunoprecipitation. Cells can also be transfected with high efficiency. | España
Genetic control of immunity to Heligmosomoides polygyrus: Presentation of promiscuous antigens to parasite-specific T cell...
... using a number of T cell hybridomas produced in A and E positive and negative mice. By using fixed and irradiated antigen ... using a number of T cell hybridomas produced in A and E positive and negative mice. By using fixed and irradiated antigen ... using a number of T cell hybridomas produced in A and E positive and negative mice. By using fixed and irradiated antigen ... using a number of T cell hybridomas produced in A and E positive and negative mice. By using fixed and irradiated antigen ...
Growth of hybridoma cells under different agitation conditions - Fingerprint
- [email protected]
Anti-Stagonospora nodorum hybridoma - Oxford University Innovation
Stagonospora nodorum is an economically important foliar pathogen of cereals. The information presented here on these pages has been provided by the scientists and academics who have created the reagents. As far as Oxford University Innovation is able to establish this information is accurate but no representation is given as to its accuracy or completeness. It is the responsibility of the customer to determine the suitability for any purpose of the reagents and Oxford University Innovation shall not be liable for any claims, losses, liabilities, expenses or damages arising from any use of the materials. Nothing on these pages will form part of any contract or licence nor be regarded as a warranty or representation in relation to the products. Any supply would be subject to the terms and conditions of a separate agreement. Oxford University Innovation makes no representation that any item in this catalogue is not subject to third party intellectual property rights.. ...
Glycosylation of antibody in a lectin-resistant human hybridoma is insensitive to glucose<...
Tachibana H, Ushio Y, Murakami H. Glycosylation of antibody in a lectin-resistant human hybridoma is insensitive to glucose. In ... Tachibana, H, Ushio, Y & Murakami, H 1995, Glycosylation of antibody in a lectin-resistant human hybridoma is insensitive to ... Glycosylation of antibody in a lectin-resistant human hybridoma is insensitive to glucose. / Tachibana, Hirofumi; Ushio, ... Tachibana, H., Ushio, Y., & Murakami, H. (1995). Glycosylation of antibody in a lectin-resistant human hybridoma is insensitive ...
Human antibodies and hybridomas
Results of search for 'su:{Hybridomas}' › WHO HQ Library catalog
Hybridoma technology with special reference to parasitic diseases. by UNDP/World Bank/WHO Special Programme for Research and ... Hybridoma technology with special reference to parasitic diseases : a notebook of papers and techniques presented at two ... Properties of the monoclonal antibodies produced by hybridoma technology and their application to the study of diseases : ... Properties of the monoclonal antibodies produced by hybridoma technology and their application to the study of diseases : ...
Details for: Monoclonal antibodies and T-cell hybridomas : › WHO HQ Library catalog
Catalog #24102 - Cloning and sequencing of rat hybridoma IgG variable regions - Curia Global
Cloning and sequencing of rat hybridoma IgG variable regions ... Cloning and sequencing of rat hybridoma IgG variable regions. ... 1. Extract and purify total RNA from hybridoma cells or clonal B cell. ... Catalog #24101 - Cloning and sequencing of mouse hybridoma IgG variable regions ... Catalog #24103 - Cloning and sequencing of rabbit hybridoma IgG variable regions ...
Hybridoma Sequencing Pitfalls - Abterra Biosciences
Learn about common mistakes made during hybridoma sequencing projects. ... Impurity of cells/hybridomas: Diluting and culturing hybridomas as monoclonal is not always accurate, so a sample can ... Abterra Bio uses NGS for hybridoma sequencing and our software can assess clonality of your hybridomas. We will give you access ... Not all hybridomas produce monoclonal antibodies. In addition, a sample could have contamination of other organisms like ...
Core - Hybridoma/Immunohistochemistry - Carol Wikstrand
Monoclonal Antibody Development | Hybridoma Development | Antibody Generation
Hybridoma & Cell Lines Rescue Services , Immune Response Assays , Monoclonal Antibody & Hybridoma Development Services , ... Monoclonal Antibody and Hybridoma Development Services. One of our core competency and expertise is in development of new ... hybridoma monoclonal antibody. Our key personals have over 25 years of expertise in this area, making many monoclonal ...
China good quality Custom Monoclonal Antibody on sales
Methadone Hybridoma Metabolization Monoclonal Anti body from China, Chinas leading mouse monoclonal antibodies product, with ... Custom Monoclonal Antibody Hybridoma Monoclonal Antibody Mouse Monoclonal Antibodies Drug Abuse Test Kit Rapid Test Kit ... Mouse anti-Methadone Hybridoma Monoclone Antibody Drug of Abuse For In Vitro Research ... Mouse anti-Methadone Hybridoma Monoclone Antibody is the high performance product to meet ...
Science Clips - Monday, July 19, 2010
Semi-solid cloning of mouse hybridomas to generate mouse monoclonal antibodies
This video outlines the steps in semi-solid cloning of mouse hybridomas to generate mouse monoclonal antibodies using ClonaCell ... This video outlines the steps in semi-solid cloning of mouse hybridomas to generate mouse monoclonal antibodies using ClonaCell ... Semi-solid cloning of mouse hybridomas to generate mouse monoclonal antibodies. March 17, 2014 ...
In vitro antibody synthesis in 20 μl hanging drops: Initiation of secondary responses and a simple method of cloning hybridomas...
In vitro antibody synthesis in 20 μl hanging drops: Initiation of secondary responses and a simple method of cloning hybridomas ... In vitro antibody synthesis in 20 μl hanging drops: Initiation of secondary responses and a simple method of cloning hybridomas ... The hanging drop method also provides a reliable yet simple procedure of cloning hybridoma cells while permitting direct ... The hanging drop method also provides a reliable yet simple procedure of cloning hybridoma cells while permitting direct ...
Hybridoma Sequencing Service - KMD Bioscience-Professional CRO Service Supplier
The acquisition of the monoclonal antibody gene sequence of hybridoma cells is the basis for recombinant antibodies and the ... Hybridoma Sequencing Service. The acquisition of the monoclonal antibody gene sequence of hybridoma cells is the basis for ... Customers can provide antigen and hybridoma cell lines directly. --Customers can provide antigens, hybridoma cell culture ... In the process of preparing recombinant antibodies, after customer supplies the hybridoma cells, there are two heavy chain and ...
Difference between revisions of "Hybridoma cells clones" - Tanaka Wiki
for cloning: use Hybridoma cloning factor (HCF) (Hybrid - max Hybridoma media supplement #ECO1021N (50 ml)) use @ 10 % ... Thawing of Hybridoma cells 1) thaw a vial of cells quickly @ 37 degree 2) transfer into 10 ml fresh High serum media 3) ... Passaging of Hybridoma cells Materials: - HS- media - 15 ml falcon tube - 25 cm2 corning cell culture flasks (Pax 7 cells need ... Thaw the 1st AB (f.ex.: mouse anti- Pax7 - Hybridoma supernatants) @Rt or ...
AntibodiesCellsSpecificityPhage displayAntigenClonesClonalityMiceAssayCloningProliferationCulturesTechnologyCovalentReferencesMediumHumanPageCoreTechniqueAntibodiesAntibodySupernatantDEVELOPMENTAL STUDHelper T cell hybridomConjugate1984MabsTechnologySuppressorSerum-FreeCharacterizationCloneViabilityGenerationVitroLymphocyteProduction1989ProteinsSuitableFacilityFactorSegmentGene
Antibodies25
- Hybridoma technology is a method for mass-producing antibodies in a hybrid cell line generated from the fusion of antibody-producing B-cells with an immortalized myeloma cell line, now called a hybridoma cell. (moleculardevices.com)
- Because every B-cell produces a unique antibody, single-cell cloning of hybridomas can be used to generate a diverse library of unique monoclonal antibodies at a large scale, which are very frequently used in the prevention, diagnosis, and treatment of disease. (moleculardevices.com)
- The process of fusing B cells, expressing unique antibodies, with myeloma cells creating a hybrid cell line is called a hybridoma. (moleculardevices.com)
- The identification of clonally-derived hybridoma cell lines which are producing high amounts of monoclonal antibodies. (moleculardevices.com)
- To address these issues we developed a novel selective technology based on an artificial cell surface construct by which secreted antibodies were connected to the corresponding hybridoma cell when they possess the desired antigen-specificity. (nature.com)
- Especially the hybridoma technique which results in full-length monoclonal antibodies can be cumbersome, labour-intensive and time-consuming (Fig. 1A ). (nature.com)
- To facilitate the isolation of specific antibody-producing hybridomas, a method has to be established which temporarily restricts the cells from releasing the antibody into the culture medium and thus retaining the genotype (the antibody-coding genes) and the phenotype (the produced antibodies) in one entity. (nature.com)
- Human antibodies and hybridomas / Butterworth Heinemann Publishers. (bvs.br)
- Monoclonal antibodies and T-cell hybridomas : perspectives and technical advances / editors, Günter J. Hämmerling, Ulrich Hämmerling and John F. Kearney. (who.int)
- NGS provides greater sequencing depth and would be able to capture polyclonal antibodies from a hybridoma sample. (abterrabio.com)
- Not all hybridomas produce monoclonal antibodies. (abterrabio.com)
- 1999. 1, Generation of Hybridomas: Permanent Cell Lines Secreting Monoclonal Antibodies. (abterrabio.com)
- This video outlines the steps in semi-solid cloning of mouse hybridomas to generate mouse monoclonal antibodies using ClonaCell™-HY. (cellculturedish.com)
- The acquisition of the monoclonal antibody gene sequence of hybridoma cells is the basis for recombinant antibodies and the development of antibody drugs. (kmdbioscience.com)
- In the process of preparing recombinant antibodies, after customer supplies the hybridoma cells, there are two heavy chain and two light chain problems, which leads to the diversity of subsequent light and heavy chain pairing combinations, making the preparation of recombinant antibodies time-consuming and labor-intensive. (kmdbioscience.com)
- King, G 2014, ' Klotho MAb: Hybridoma Lines KL-115 and KL-234 ', Monoclonal Antibodies in Immunodiagnosis and Immunotherapy , vol. 33, no. 6, pp. 449. (elsevier.com)
- The Antibody and Bioconjugates Discovery group at NIBR/NBC-San Diego is seeking an antibody expert to lead a powerful hybridoma platform for discovery of monoclonal antibodies. (gijobs.com)
- Our RabMAb ® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. (abcam.com)
- Thesis: Investigation of the hybridomas stabilities and neutralization capabilities of monoclonal antibodies developed against the glycoproteins of Crimean-Congo Hemorrhagic Fever Virus (CCHFV). (uni-muenster.de)
- VICTORIA, July 13, 2020 / - IMMUNOPRECISE ANTIBODIES LTD. (the "Company" or "IPA") (TSX VENTURE: IPA) (OTCQB: IPATF) (FSE:TQB2), a leader in full-service, therapeutic antibody discovery and development, today announced the identification of additional, human lead candidate antibodies generated against SARS-CoV-2, also demonstrating potent in vitro neutralizing activity, discovered using the Company's B cell Select™ and Single Step Cloning Hybridoma Platforms. (ipatherapeutics.com)
- More particularly it concerns murine monoclonal anti-human breast cancer antibodies, hybridomas that produce those antibodies, immunochemicals made from those antibodies, and diagnostic and therapeutic methods that use those immunochemicals. (justia.com)
- The murine x murine hybridomas that produce the above described antibodies are progeny of those hybridomas are other aspects of the invention. (justia.com)
- The affinity and kinetics of monoclonal antibodies produced from a hybridoma cell line cultured in two different types of medium were measured. (biopharminternational.com)
- In this study, the authors evaluate the Octet QKe platform to establish an analytical method to assess media condition effect on the quality and activity of cultured antibodies by measuring the affinity and kinetics of monoclonal antibodies (mAbs) produced from a hybridoma cell line cultured in two different types of medium. (biopharminternational.com)
- These homophilic antibodies can be immortalized as monoclonal antibodies using the hybridoma or phage display technique. (medscape.com)
Cells14
- Anti-α-Tubulin antibody, Mouse monoclonal (mouse IgG1 isotype) is derived from the hybridoma DM1A produced by the fusion of mouse myeloma cells (NS1) and splenocytes from BALB/c mice immunized with purified chick brain tubulin. (sigmaaldrich.com)
- The stably transfected myeloma cell line was used for the generation of hybridoma cells and an antigen- and isotype-specific screening method was established. (nature.com)
- The picture (reprinted by permission from Springer Nature 10 ) shows the process of monoclonal antibody generation via conventional hybridoma technology ( A ) and via the new selection approach using transgenic fusion cell lines ( B ). The fusion with transgenic myeloma cells allows a fast and efficient hybridoma screening in an isotype- or antigen-specific manner and allows an early screening for possible cross-reactivities. (nature.com)
- Cloning hybridomas and CHO cells can be done faster and more efficiently using semi-solid cloning. (stemcell.com)
- 1. Extract and purify total RNA from hybridoma cells or clonal B cell. (curiaglobal.com)
- The hanging drop method also provides a reliable yet simple procedure of cloning hybridoma cells while permitting direct visualization of the number of cells in each drop. (elsevier.com)
- Mouse hybridoma cells producing monoclonal antibody against the 1465 antigenic particles of O type apthhoviruses were proliferated on the non-woven polyester fabric (NWPF) discs in stationary and stirred conditions. (hacettepe.edu.tr)
- In this study, we established stable transfectants expressing two types of activin receptors, ActRI and ActRIB, to clarify the role of these receptors in activin signalling for growth inhibition in HS-72 mouse B-cell hybridoma cells. (elsevier.com)
- These fused cells, called hybridomas, could be grown in culture. (si.edu)
- Hybridoma cells: Yes. (cdc.gov)
- A. W. Hohmann, L. Spatz, M. Irigoyen and A. Manheimer-Lory -- Introduction -- Immortalized B cells to probe the human antibody repertoire -- Infectious diseases -- Human immunodeficiency virus -- Erythrocyte antigens -- Human histocompatibility antigens -- Tumor antigens -- Autoantibodies -- Immortalization of B cells with EBV -- EBV transformation of B cells: methodology -- Making human hybridomas -- Hybridoma methodology -- References -- 4. (routledge.com)
- Immune splenocytes were fused with mouse myeloma cells and hybridomas were selected based on some specificity of the culture media for breast or breast cancer antigens. (justia.com)
- specimins, hybridoma cells, display cases. (cooperhewitt.org)
- Hybridoma clones have been derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice immunized with recombinant SARS-CoV-2 Nucleoprotein (C518, C524, C527). (hytest.fi)
Specificity1
- Specificity: Hybridoma tissue culture supernatants (CSN) were screened using an indirect ELISA as previously described. (cdc.gov)
Phage display1
- Superseding hybridoma technology with phage display libraries. (routledge.com)
Antigen1
- The critical issue in the development of antigen-specific hybridomas is the lack of any direct connection between the hybridoma cell and the released antibody. (nature.com)
Clones2
- Our proprietary Mouse Quick-Hybridoma™ Platform allows generation of 50+ hybridoma clones per hybridoma cell fusion step. (advbiomart.net)
- This platform is developed based on our new mouse adjuvant, FastAb™ Mouse Adjuvant , which enables generation of a large number of hybridoma clones using less immunogens. (advbiomart.net)
Clonality1
- Abterra Bio uses NGS for hybridoma sequencing and our software can assess clonality of your hybridomas. (abterrabio.com)
Mice2
- The role of MHC class II in the presentation of Heligmosomoides polygyrus antigens has been investigated, using a number of T cell hybridomas produced in A and E positive and negative mice. (elsevier.com)
- A final boost of 50 mu g was given 3 weeks after the sixth immunization, and mice were sacrificed 3 days later for hybridoma production. (cdc.gov)
Assay1
- All of these delta hIL-6 proteins were inactive in the IL-6 hybridoma growth factor (HGF) assay and unable to inhibit the HGF activity of the recombinant human wild-type IL-6 (wt hIL-6). (uniparthenope.it)
Cloning1
- Bell, EB, Brown, M & Rittenberg, MB 1983, ' In vitro antibody synthesis in 20 μl hanging drops: Initiation of secondary responses and a simple method of cloning hybridomas ', Journal of Immunological Methods , vol. 62, no. 2, pp. 137-145. (elsevier.com)
Proliferation1
- Complex subunit associated factors are involved in hybridoma growth, Eosinohils, eritroid proliferation and derived from promotor binding stimulating subunits on the DNA binding complex. (elisachina.com)
Cultures1
- The medium elevates glucose and glutamine levels to deliver nutrients to the tissue-dense hollow-fiber hybridoma cultures. (the-scientist.com)
Technology2
- Schematic overview about conventional hybridoma technology compared side by side to the new selection approach. (nature.com)
- Hybridoma technology with special reference to parasitic diseases. (who.int)
Covalent1
- To better understand the physical basis of the recognition stage of Ag presentation, we examined adhesion of the lysozyme- specific T cell hybridoma, 3A9, to artificial bilayers containing covalent MHC class II/peptide complexes or adhesion molecules. (nih.gov)
References1
- In this video , Justin Dranschak, manager for BioPharma platforms, presents our solution for a hybridoma workflow and references the systems to aid in your research. (moleculardevices.com)
Medium1
- Unisyn Technologies, based in San Diego, has introduced Hybrid Grow specialized basal medium for growing hybridomas in the company's Cell-Pharm hollow-fiber bioreactors. (the-scientist.com)
Human2
- Tachibana, H , Ushio, Y & Murakami, H 1995, ' Glycosylation of antibody in a lectin-resistant human hybridoma is insensitive to glucose ', In Vitro Cellular & Developmental Biology - Animal: Journal of the Society for In Vitro Biology , vol. 31, no. 4, pp. 261-262. (elsevier.com)
- Description: Description of target: The human interferon-beta 2 gene (IFNB2) product is identical to that for the B-cell stimulation factor-2 (BSF-2), the hybridoma growth factor (HGF) ("interleukin-6"), and the hepatocyte stimulating factor (HSF). (ayurvedandindia.com)
Page1
- La liste des publications du groupe antérieure à 2014 est visible en bas de page (données saisies de façon manuelle). (ibs.fr)
Core1
- One of our core competency and expertise is in development of new hybridoma monoclonal antibody. (antibodyresearch.com)
Technique1
- To confer this basic principle to the hybridoma technique would require to capture the synthesized antibody on the surface of the synthesizing hybridoma cell (Fig. 1B ). (nature.com)
Antibodies22
- The hybridoma collection of IZSLER Biobank contains an initial selection of those hybridoma that produce monoclonal antibodies regularly used in in-house diagnostic assays. (ibvr.org)
- The hybridoma currently included in the Biobank are a sample of the many hybridoma generated during 30 years of research and development activities conducted at IZSLER to produce monoclonal antibodies against a wide spectrum of viruses, bacteria and proteins, mainly of veterinary interest, that were shown to be strategic tools for both research and diagnostic purposes. (ibvr.org)
- The monoclonal antibodies expressed by each hybridoma are controlled through a series of immunological assays aimed at identifying and characterising their reactivity profile. (ibvr.org)
- Moreover, the hybrid cell, called a hybridoma, pro-duces and secretes antibodies characteristic of the clone from which the normal lymphocyte is taken. (biologydiscussion.com)
- Dr. Jean-Christophe Bourdon, Head of the p53 isoform laboratory at the University of Dundee, and his team have been using a CellMaker Plus 8L system to effectively produce a range of p53 antibodies from murine hybridoma cells. (cellexus.com)
- B cell hybridomas expressing class I and II MHC molecules and producing antibodies directed against hemagglutinin protein of Rinderpest virus and human Mucin-1 have been used as surrogate B cells to study T cell responses against the antigens. (iisc.ac.in)
- The hybridoma technology allows the production of large quantities of specific antibodies of a single isotype. (elsevier.com)
- The critical chapters on generating monoclonal antibodies and growing hybridomas, which demystified hybridoma generation, have been greatly expanded and updated to make these procedures easy to follow and adaptable to current research needs. (cshlpress.com)
- As Dr. Greenfield notes in his preface to this second edition: "The Antibodies manual provided our laboratory with guidance in the form of protocols and recommendations for setting up a hybridoma facility. (cshlpress.com)
- Monoclonal antibodies can be produced using hybridomas. (labmanager.com)
- We can isotype and sequence mouse or rat antibodies from hybridoma cell lines generated at the facility as well as cell lines from outside sources. (monash.edu)
- Human Antibodies is an international journal designed to bring together all aspects of human hybridomas and antibody technology, along with factors that modulate host antibody repertoire and effectiveness, such as vaccines, infectious agents, and microbiome. (iospress.com)
- There she learned the techniques of working with live cells, cell biology, hybridoma technology, and monoclonal antibodies. (nih.gov)
- We report here the generation of hybridomas secreting monoclonal antibodies to rat lung fibroblast-pneumonocyte factor. (elsevier.com)
- Recombinant antibodies have many advantages when compared to the typical hybridoma technology for producing monoclonal antibodies. (nih.gov)
- The resultant hybrid cell or hybridoma has the capacity to produce antibodies of predetermined specificity and to grow "immortally" in culture. (irg-wp.com)
- In contrast, hybridoma-based systems for producing monoclonal antibodies are subject to genetic drift and instability, increasing the potential for lot-to-lot variability or loss of antibody expression. (cellsignal.com)
- Where polyclonal antibodies are purified directly from the serum of the immunized host, and monoclonals are purified from either hybridoma-derived tissue culture supernatant or ascites, recombinant antibodies are instead purified from the tissue culture supernatants of transfected host cell lines. (cellsignal.com)
- A hybridoma cell can secrete and reproduce antibodies while proliferating indefinitely. (creativitea.org)
- Monoclonal antibodies were produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, E. coli-derived, recombinant human IFNg. (sbhsciences.com)
- Antibodies may also be defined through a deposited hybridoma cell producing the antibodies. (bdl-ip.com)
- In Oct 2018, Immunoprecise Antibodies Ltd., one of the major player in life science market signed an agreement with 15 pharmaceutical companies to develop novel antibodies with the help of company's hybridoma technology. (fortunebusinessinsights.com)
Antibody14
- Antigen-specific and antibody-mediated growth inhibition of suppressor T cell hybridomas. (harvard.edu)
- Hybridoma are submitted to a double series of cloning procedure to assure the stability of the hybridoma and the clonality of the antibody produced. (ibvr.org)
- To determine the sequence of the MAb, the cDNA of your antibody can be isolated from the hybridoma cell line and its subcloning and expression in mammalian cells. (exonbio.com)
- Mouse anti- beta HCG Mab Hybridoma Monoclone Antibody is the high performance product to meet your different biological research and manufactory requirements. (custom-monoclonalantibody.com)
- A series of human hybridomas were derived by the fusion of the immature T cell line , 1M , with lymphocytes from the peripheral blood of a colon carcinoma patient in long term remission who was shown to produce anti-tumor antibody . (musc.edu)
- These hybridomas express the T cell surface markers CD2 , CD3 , CD4 , and CD8 and secrete human IgG2/kappa antibody . (musc.edu)
- ClonaCell™-HY AOF Expansion Medium promotes robust expansion of hybridomas, adaptation from serum-containing to serum-free media, and stabilizing the viability and antibody productivity of hybridoma cell lines. (stemcell.com)
- The absence of serum in this medium facilitates detection and purification of the desired hybridoma-derived antibody without interference from serum-derived immunoglobulins. (stemcell.com)
- At first, VH and VL gene sequences were cloned from hybridoma cell lines secreting monoclonal antibody against LMG, and then thoroughly by database-assisted sequence analysis. (nih.gov)
- The antibody sequencing protocol involves isolating the mRNA from hybridoma cells followed by cDNA synthesis and PCR amplification of heavy- and light-chain variable region genes. (monash.edu)
- High-throughput screening for the identification of antibody hits in culture supernatants of hybridoma clones is usually performed in microtiter plates (MTPs) with 96 or more wells. (eppendorf.com)
- Crystallization chaperones generated using recombinant technologies have emerged as superior alternatives that increase the throughput and eliminate inherent limitations associated with antibody production by animal immunization and the hybridoma technology. (nih.gov)
- Diatec produces our very own fully human IgE antibody from a monoclonal hybridoma cell line. (diatec.com)
- This antibody was produced from a hybridoma (mouse myeloma fused with spleen cells from a rat) immunized with mouse recombinant protein of CCL-25. (reliatech.de)
Supernatant2
- We injected the hybridoma cells into the peritoneal cavity of pristane-primed F1 (AKR/J X BALB/c) mice, and a large amount of pure MNSF was obtained from the ascites, the characteristics of which were similar to those in the culture supernatant. (jimmunol.org)
- Purified from hybridoma tissue culture supernatant. (enzolifesciences.com)
DEVELOPMENTAL STUD1
- The reagents produced by this group are distributed at a minimal cost by the Developmental Studies Hybridoma Bank , ensuring broad availability and wide accessibility. (nih.gov)
Helper T cell hybridom1
- cDNA libraries were constructed from one suppressor and two helper T cell hybridomas. (rupress.org)
Conjugate1
- Hybridomas were screened in a solid-phase indirect enzyme-linked immunosorbent assay (ELISA) against a 2,4 TDI-human serum albumin (2,4 TDI-HSA) protein conjugate. (cdc.gov)
19841
- 1984. Hybridoma 3:301. (biolegend.com)
Mabs2
- Three hybridomas producing 2,4 TDI-HSA reactive IgM MAbs were obtained. (cdc.gov)
- Moreover, hybridomas production or generation of mAbs usually takes around ten months. (creativitea.org)
Technology3
- in Hybridoma Technology with Special Reference to Parasite Diseases, UNDP/World Bank, Geneva 1979, p. 65. (crossref.org)
- Hybridoma technology was the first mAb production method that allowed for in vivo scientific research and is still the primary and preferred platform for mAb derivatisation (3). (cellexus.com)
- I am a Fungal Immunologist with a specialist interest in hybridoma technology. (exeter.ac.uk)
Suppressor1
- Isolation and characterization of a monoclonal nonspecific suppressor factor (MNSF) produced by a T cell hybridoma. (jimmunol.org)
Serum-Free2
- ClonaCell™-HY AOF Expansion Medium is an animal origin-free (AOF) and serum-free liquid medium optimized for hybridoma expansion after hypoxanthine, aminopterin, thymidine (HAT) selection. (stemcell.com)
- ab110304 was produced in vitro using hybridomas grown in serum-free medium. (abcam.com)
Characterization2
- Iglesias-Ussel, MD, Zavadil, J & Scharff, MD 2009, ' Molecular characterization of hybridoma subclones spontaneously switching at high frequencies in vitro ', Journal of Immunological Methods , vol. 350, no. 1-2, pp. 71-78. (elsevier.com)
- Five of these hybridomas selected for further characterization are the subject of this dissertation . (musc.edu)
Clone2
- With this method, a stable E17 hybridoma clone was selected, and its product in culture medium was isolated and characterized. (jimmunol.org)
- It is often observed that more than one clone is found in a monoclonal hybridoma cell line. (exonbio.com)
Viability1
- In most cases, cryopreserved hybridomas can be thawed directly into ClonaCell™-HY AOF Expansion Medium while maintaining a high level of viability. (stemcell.com)
Generation2
Vitro2
- We have shown previously that forced expression of activation induced cytidine deaminase (AID) in hybridomas increased their very low frequency of class switch recombination (CSR) in vitro only ∼ 7-13 fold. (elsevier.com)
- Since we had previously identified rare hybridoma subclones that spontaneously switched at more than 100 times higher frequencies, we have now examined those higher switching variants to search for ways to further increase the frequency of isotype switching in vitro. (elsevier.com)
Lymphocyte1
- The hybridomas will proliferate because the lymphocyte nuclei con-tribute functional HGPRT genes to the heterokaryon. (biologydiscussion.com)
Production2
- In this report we describe a functional role for Shc in two events that occur during T cell activation, AICD and IL-2 production, in a T cell hybridoma line that has been previously shown to undergo apoptosis and produce IL-2 upon cross-linking of the TCR/CD3 complex. (jimmunol.org)
- Splenocytes were isolated 24 h after the last exposure for hybridoma production. (cdc.gov)
19891
- 1989. Hybridoma 8:605. (biolegend.com)
Proteins1
- Thus, the MNSF obtained from the E17 hybridoma consists of functionally identical but physicochemically different discrete proteins. (jimmunol.org)
Suitable1
- The particular hybridomas producing the desired anti-body can be identified using a suitable assay proce-dure, and these can be selected and separately cloned. (biologydiscussion.com)
Facility1
- Paul Kapke, an associate scientist in the Office of Biotechnology and manager of the Hybridoma Facility, is retiring May 31. (iastate.edu)
Factor1
- A cytokine that stimulates the growth and differentiation of B-LYMPHOCYTES and is also a growth factor for HYBRIDOMAS and plasmacytomas. (nih.gov)
Segment1
- The hybridomas segment is also expected to register considerable revenue growth in the years to come. (pharmiweb.com)
Gene3
- Three T cell hybridomas do not contain detectable heavy chain variable gene transcripts. (rupress.org)
- AID was not responsible for the ∼ 100 fold increase in CSR, so we used whole-genome gene expression profiling to provide a platform for studying candidate molecular pathways underlying spontaneous CSR in hybridomas. (elsevier.com)
- CD40L is discussed in relation to a potential role in supporting B cell tumors and it has been discovered that the molecular defect in the X-linked Hyper-IgM-Syndrome is targeted to the CD40L gene, it is functional involved in B cell hybridomas and chronic lymphocytic leukemia as well as several autoimmune diseases. (diaclone.com)