Nucleic Acid Hybridization
Widely used technique which exploits the ability of complementary sequences in single-stranded DNAs or RNAs to pair with each other to form a double helix. Hybridization can take place between two complimentary DNA sequences, between a single-stranded DNA and a complementary RNA, or between two RNA sequences. The technique is used to detect and isolate specific sequences, measure homology, or define other characteristics of one or both strands. (Kendrew, Encyclopedia of Molecular Biology, 1994, p503)
In Situ Hybridization
In Situ Hybridization, Fluorescence
Hybridization, Genetic
DNA Probes
Species- or subspecies-specific DNA (including COMPLEMENTARY DNA; conserved genes, whole chromosomes, or whole genomes) used in hybridization studies in order to identify microorganisms, to measure DNA-DNA homologies, to group subspecies, etc. The DNA probe hybridizes with a specific mRNA, if present. Conventional techniques used for testing for the hybridization product include dot blot assays, Southern blot assays, and DNA:RNA hybrid-specific antibody tests. Conventional labels for the DNA probe include the radioisotope labels 32P and 125I and the chemical label biotin. The use of DNA probes provides a specific, sensitive, rapid, and inexpensive replacement for cell culture techniques for diagnosing infections.
Base Sequence
Molecular Sequence Data
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
RNA, Messenger
RNA sequences that serve as templates for protein synthesis. Bacterial mRNAs are generally primary transcripts in that they do not require post-transcriptional processing. Eukaryotic mRNA is synthesized in the nucleus and must be exported to the cytoplasm for translation. Most eukaryotic mRNAs have a sequence of polyadenylic acid at the 3' end, referred to as the poly(A) tail. The function of this tail is not known for certain, but it may play a role in the export of mature mRNA from the nucleus as well as in helping stabilize some mRNA molecules by retarding their degradation in the cytoplasm.
Comparative Genomic Hybridization
Polymerase Chain Reaction
In vitro method for producing large amounts of specific DNA or RNA fragments of defined length and sequence from small amounts of short oligonucleotide flanking sequences (primers). The essential steps include thermal denaturation of the double-stranded target molecules, annealing of the primers to their complementary sequences, and extension of the annealed primers by enzymatic synthesis with DNA polymerase. The reaction is efficient, specific, and extremely sensitive. Uses for the reaction include disease diagnosis, detection of difficult-to-isolate pathogens, mutation analysis, genetic testing, DNA sequencing, and analyzing evolutionary relationships.
Cloning, Molecular
RNA Probes
RNA, usually prepared by transcription from cloned DNA, which complements a specific mRNA or DNA and is generally used for studies of virus genes, distribution of specific RNA in tissues and cells, integration of viral DNA into genomes, transcription, etc. Whereas DNA PROBES are preferred for use at a more macroscopic level for detection of the presence of DNA/RNA from specific species or subspecies, RNA probes are preferred for genetic studies. Conventional labels for the RNA probe include radioisotope labels 32P and 125I and the chemical label biotin. RNA probes may be further divided by category into plus-sense RNA probes, minus-sense RNA probes, and antisense RNA probes.
Oligonucleotide Probes
Synthetic or natural oligonucleotides used in hybridization studies in order to identify and study specific nucleic acid fragments, e.g., DNA segments near or within a specific gene locus or gene. The probe hybridizes with a specific mRNA, if present. Conventional techniques used for testing for the hybridization product include dot blot assays, Southern blot assays, and DNA:RNA hybrid-specific antibody tests. Conventional labels for the probe include the radioisotope labels 32P and 125I and the chemical label biotin.
Sequence Analysis, DNA
RNA, Ribosomal, 16S
Blotting, Southern
DNA
A deoxyribonucleotide polymer that is the primary genetic material of all cells. Eukaryotic and prokaryotic organisms normally contain DNA in a double-stranded state, yet several important biological processes transiently involve single-stranded regions. DNA, which consists of a polysugar-phosphate backbone possessing projections of purines (adenine and guanine) and pyrimidines (thymine and cytosine), forms a double helix that is held together by hydrogen bonds between these purines and pyrimidines (adenine to thymine and guanine to cytosine).
DNA, Ribosomal
Chromosome Aberrations
Chromosome Mapping
Amino Acid Sequence
Oligonucleotide Array Sequence Analysis
Digoxigenin
Blotting, Northern
Species Specificity
The restriction of a characteristic behavior, anatomical structure or physical system, such as immune response; metabolic response, or gene or gene variant to the members of one species. It refers to that property which differentiates one species from another but it is also used for phylogenetic levels higher or lower than the species.
DNA, Complementary
Gene Amplification
A selective increase in the number of copies of a gene coding for a specific protein without a proportional increase in other genes. It occurs naturally via the excision of a copy of the repeating sequence from the chromosome and its extrachromosomal replication in a plasmid, or via the production of an RNA transcript of the entire repeating sequence of ribosomal RNA followed by the reverse transcription of the molecule to produce an additional copy of the original DNA sequence. Laboratory techniques have been introduced for inducing disproportional replication by unequal crossing over, uptake of DNA from lysed cells, or generation of extrachromosomal sequences from rolling circle replication.
Immunohistochemistry
Gene Expression
Gene Dosage
The number of copies of a given gene present in the cell of an organism. An increase in gene dosage (by GENE DUPLICATION for example) can result in higher levels of gene product formation. GENE DOSAGE COMPENSATION mechanisms result in adjustments to the level GENE EXPRESSION when there are changes or differences in gene dosage.
Sequence Homology, Nucleic Acid
Genes
Transcription, Genetic
Gene Library
RNA, Bacterial
DNA Primers
Chromosome Banding
Staining of bands, or chromosome segments, allowing the precise identification of individual chromosomes or parts of chromosomes. Applications include the determination of chromosome rearrangements in malformation syndromes and cancer, the chemistry of chromosome segments, chromosome changes during evolution, and, in conjunction with cell hybridization studies, chromosome mapping.
RNA
A polynucleotide consisting essentially of chains with a repeating backbone of phosphate and ribose units to which nitrogenous bases are attached. RNA is unique among biological macromolecules in that it can encode genetic information, serve as an abundant structural component of cells, and also possesses catalytic activity. (Rieger et al., Glossary of Genetics: Classical and Molecular, 5th ed)
DNA Restriction Enzymes
Enzymes that are part of the restriction-modification systems. They catalyze the endonucleolytic cleavage of DNA sequences which lack the species-specific methylation pattern in the host cell's DNA. Cleavage yields random or specific double-stranded fragments with terminal 5'-phosphates. The function of restriction enzymes is to destroy any foreign DNA that invades the host cell. Most have been studied in bacterial systems, but a few have been found in eukaryotic organisms. They are also used as tools for the systematic dissection and mapping of chromosomes, in the determination of base sequences of DNAs, and have made it possible to splice and recombine genes from one organism into the genome of another. EC 3.21.1.
Gene Expression Profiling
Restriction Mapping
Aneuploidy
The chromosomal constitution of cells which deviate from the normal by the addition or subtraction of CHROMOSOMES, chromosome pairs, or chromosome fragments. In a normally diploid cell (DIPLOIDY) the loss of a chromosome pair is termed nullisomy (symbol: 2N-2), the loss of a single chromosome is MONOSOMY (symbol: 2N-1), the addition of a chromosome pair is tetrasomy (symbol: 2N+2), the addition of a single chromosome is TRISOMY (symbol: 2N+1).
Reverse Transcriptase Polymerase Chain Reaction
Gene Expression Regulation, Developmental
Translocation, Genetic
Sensitivity and Specificity
Phenotype
Sequence Homology, Amino Acid
Molecular Probe Techniques
Plasmids
Nucleic Acid Probes
Genes, rRNA
Bacterial Typing Techniques
Peptide Nucleic Acids
DNA analogs containing neutral amide backbone linkages composed of aminoethyl glycine units instead of the usual phosphodiester linkage of deoxyribose groups. Peptide nucleic acids have high biological stability and higher affinity for complementary DNA or RNA sequences than analogous DNA oligomers.
Tissue Distribution
Accumulation of a drug or chemical substance in various organs (including those not relevant to its pharmacologic or therapeutic action). This distribution depends on the blood flow or perfusion rate of the organ, the ability of the drug to penetrate organ membranes, tissue specificity, protein binding. The distribution is usually expressed as tissue to plasma ratios.
Gene Expression Regulation
Chromosomes, Human, Pair 1
Chromosomes, Human
Chromosomes, Artificial, Bacterial
Y Chromosome
RNA, Ribosomal
The most abundant form of RNA. Together with proteins, it forms the ribosomes, playing a structural role and also a role in ribosomal binding of mRNA and tRNAs. Individual chains are conventionally designated by their sedimentation coefficients. In eukaryotes, four large chains exist, synthesized in the nucleolus and constituting about 50% of the ribosome. (Dorland, 28th ed)
Oligonucleotides
Chromosomes, Human, Pair 7
Sequence Alignment
The arrangement of two or more amino acid or base sequences from an organism or organisms in such a way as to align areas of the sequences sharing common properties. The degree of relatedness or homology between the sequences is predicted computationally or statistically based on weights assigned to the elements aligned between the sequences. This in turn can serve as a potential indicator of the genetic relatedness between the organisms.
Chromosomes, Human, Pair 17
Repetitive Sequences, Nucleic Acid
Sequences of DNA or RNA that occur in multiple copies. There are several types: INTERSPERSED REPETITIVE SEQUENCES are copies of transposable elements (DNA TRANSPOSABLE ELEMENTS or RETROELEMENTS) dispersed throughout the genome. TERMINAL REPEAT SEQUENCES flank both ends of another sequence, for example, the long terminal repeats (LTRs) on RETROVIRUSES. Variations may be direct repeats, those occurring in the same direction, or inverted repeats, those opposite to each other in direction. TANDEM REPEAT SEQUENCES are copies which lie adjacent to each other, direct or inverted (INVERTED REPEAT SEQUENCES).
Cytogenetic Analysis
Paraffin Embedding
Nucleic Acid Denaturation
Disruption of the secondary structure of nucleic acids by heat, extreme pH or chemical treatment. Double strand DNA is "melted" by dissociation of the non-covalent hydrogen bonds and hydrophobic interactions. Denatured DNA appears to be a single-stranded flexible structure. The effects of denaturation on RNA are similar though less pronounced and largely reversible.
Organ Specificity
Mutation
Escherichia coli
A species of gram-negative, facultatively anaerobic, rod-shaped bacteria (GRAM-NEGATIVE FACULTATIVELY ANAEROBIC RODS) commonly found in the lower part of the intestine of warm-blooded animals. It is usually nonpathogenic, but some strains are known to produce DIARRHEA and pyogenic infections. Pathogenic strains (virotypes) are classified by their specific pathogenic mechanisms such as toxins (ENTEROTOXIGENIC ESCHERICHIA COLI), etc.
Chromosomes, Human, Pair 8
Hybrid Cells
Polymorphism, Restriction Fragment Length
Chromosomes, Human, Pair 11
Genetic Markers
Cytogenetics
Electrophoresis, Agar Gel
Cosmids
Autoradiography
Chromosome Disorders
Poly A
Multigene Family
A set of genes descended by duplication and variation from some ancestral gene. Such genes may be clustered together on the same chromosome or dispersed on different chromosomes. Examples of multigene families include those that encode the hemoglobins, immunoglobulins, histocompatibility antigens, actins, tubulins, keratins, collagens, heat shock proteins, salivary glue proteins, chorion proteins, cuticle proteins, yolk proteins, and phaseolins, as well as histones, ribosomal RNA, and transfer RNA genes. The latter three are examples of reiterated genes, where hundreds of identical genes are present in a tandem array. (King & Stanfield, A Dictionary of Genetics, 4th ed)
Polyploidy
Genotype
Chromosomes
Chromosomes, Human, Pair 12
DNA, Recombinant
Chromosomes, Human, Pair 14
Gene Rearrangement
Chromosome Painting
A technique for visualizing CHROMOSOME ABERRATIONS using fluorescently labeled DNA probes which are hybridized to chromosomal DNA. Multiple fluorochromes may be attached to the probes. Upon hybridization, this produces a multicolored, or painted, effect with a unique color at each site of hybridization. This technique may also be used to identify cross-species homology by labeling probes from one species for hybridization with chromosomes from another species.
Chromosomes, Human, Pair 18
Soil Microbiology
Chromosomes, Human, Pair 3
DNA Transposable Elements
Discrete segments of DNA which can excise and reintegrate to another site in the genome. Most are inactive, i.e., have not been found to exist outside the integrated state. DNA transposable elements include bacterial IS (insertion sequence) elements, Tn elements, the maize controlling elements Ac and Ds, Drosophila P, gypsy, and pogo elements, the human Tigger elements and the Tc and mariner elements which are found throughout the animal kingdom.
Chromosomes, Human, Pair 13
Biotin
Reproducibility of Results
The statistical reproducibility of measurements (often in a clinical context), including the testing of instrumentation or techniques to obtain reproducible results. The concept includes reproducibility of physiological measurements, which may be used to develop rules to assess probability or prognosis, or response to a stimulus; reproducibility of occurrence of a condition; and reproducibility of experimental results.
X Chromosome
Brain
The part of CENTRAL NERVOUS SYSTEM that is contained within the skull (CRANIUM). Arising from the NEURAL TUBE, the embryonic brain is comprised of three major parts including PROSENCEPHALON (the forebrain); MESENCEPHALON (the midbrain); and RHOMBENCEPHALON (the hindbrain). The developed brain consists of CEREBRUM; CEREBELLUM; and other structures in the BRAIN STEM.
Cell Nucleus
Within a eukaryotic cell, a membrane-limited body which contains chromosomes and one or more nucleoli (CELL NUCLEOLUS). The nuclear membrane consists of a double unit-type membrane which is perforated by a number of pores; the outermost membrane is continuous with the ENDOPLASMIC RETICULUM. A cell may contain more than one nucleus. (From Singleton & Sainsbury, Dictionary of Microbiology and Molecular Biology, 2d ed)
Genes, erbB-2
The erbB-2 gene is a proto-oncogene that codes for the erbB-2 receptor (RECEPTOR, ERBB-2), a protein with structural features similar to the epidermal growth factor receptor. Its name originates from the viral oncogene homolog (v-erbB) which is a truncated form of the chicken erbB gene found in the avian erythroblastosis virus. Overexpression and amplification of the gene is associated with a significant number of adenocarcinomas. The human c-erbB-2 gene is located at 17q21.2.
Interphase
Cells, Cultured
Genomic Library
Transcription Factors
Papillomaviridae
A family of small, non-enveloped DNA viruses infecting birds and most mammals, especially humans. They are grouped into multiple genera, but the viruses are highly host-species specific and tissue-restricted. They are commonly divided into hundreds of papillomavirus "types", each with specific gene function and gene control regions, despite sequence homology. Human papillomaviruses are found in the genera ALPHAPAPILLOMAVIRUS; BETAPAPILLOMAVIRUS; GAMMAPAPILLOMAVIRUS; and MUPAPILLOMAVIRUS.
Protein Biosynthesis
Bacteria
One of the three domains of life (the others being Eukarya and ARCHAEA), also called Eubacteria. They are unicellular prokaryotic microorganisms which generally possess rigid cell walls, multiply by cell division, and exhibit three principal forms: round or coccal, rodlike or bacillary, and spiral or spirochetal. Bacteria can be classified by their response to OXYGEN: aerobic, anaerobic, or facultatively anaerobic; by the mode by which they obtain their energy: chemotrophy (via chemical reaction) or PHOTOTROPHY (via light reaction); for chemotrophs by their source of chemical energy: CHEMOLITHOTROPHY (from inorganic compounds) or chemoorganotrophy (from organic compounds); and by their source for CARBON; NITROGEN; etc.; HETEROTROPHY (from organic sources) or AUTOTROPHY (from CARBON DIOXIDE). They can also be classified by whether or not they stain (based on the structure of their CELL WALLS) with CRYSTAL VIOLET dye: gram-negative or gram-positive.
Chromosomes, Human, Pair 20
Water Microbiology
Tissue Fixation
Immunoenzyme Techniques
Pregnancy
Chromosomes, Human, Pair 9
Metaphase
Cluster Analysis
A set of statistical methods used to group variables or observations into strongly inter-related subgroups. In epidemiology, it may be used to analyze a closely grouped series of events or cases of disease or other health-related phenomenon with well-defined distribution patterns in relation to time or place or both.
Oligodeoxyribonucleotides
Chromosomes, Human, Pair 22
Rats, Sprague-Dawley
Biological Evolution
Tumor Virus Infections
Chromosomes, Plant
Gene Deletion
Testis
Antisense Elements (Genetics)
Embryo, Mammalian
Fatty Acids
Liver
Physical Chromosome Mapping
Mapping of the linear order of genes on a chromosome with units indicating their distances by using methods other than genetic recombination. These methods include nucleotide sequencing, overlapping deletions in polytene chromosomes, and electron micrography of heteroduplex DNA. (From King & Stansfield, A Dictionary of Genetics, 5th ed)
DNA-Binding Proteins
Reproductive Isolation
RNA, Ribosomal, 18S
Genetic Speciation
Chromosomes, Human, Pair 15
Fluorescent Dyes
Chromosomes, Human, Pair 16
Chromosomes, Human, Pair 6
Gene Expression Regulation, Neoplastic
DNA Fingerprinting
A technique for identifying individuals of a species that is based on the uniqueness of their DNA sequence. Uniqueness is determined by identifying which combination of allelic variations occur in the individual at a statistically relevant number of different loci. In forensic studies, RESTRICTION FRAGMENT LENGTH POLYMORPHISM of multiple, highly polymorphic VNTR LOCI or MICROSATELLITE REPEAT loci are analyzed. The number of loci used for the profile depends on the ALLELE FREQUENCY in the population.
Evaluation Studies as Topic
Diploidy
Embryo, Nonmammalian
Centromere
Evolution, Molecular
Genome, Human
Chromosome Breakage
Cattle
Chick Embryo
DNA Copy Number Variations
Zebrafish
Microsatellite Repeats
Expressed Sequence Tags
DNA, Satellite
Highly repetitive DNA sequences found in HETEROCHROMATIN, mainly near centromeres. They are composed of simple sequences (very short) (see MINISATELLITE REPEATS) repeated in tandem many times to form large blocks of sequence. Additionally, following the accumulation of mutations, these blocks of repeats have been repeated in tandem themselves. The degree of repetition is on the order of 1000 to 10 million at each locus. Loci are few, usually one or two per chromosome. They were called satellites since in density gradients, they often sediment as distinct, satellite bands separate from the bulk of genomic DNA owing to a distinct BASE COMPOSITION.
Alleles
Receptor, erbB-2
A cell surface protein-tyrosine kinase receptor that is overexpressed in a variety of ADENOCARCINOMAS. It has extensive homology to and heterodimerizes with the EGF RECEPTOR, the ERBB-3 RECEPTOR, and the ERBB-4 RECEPTOR. Activation of the erbB-2 receptor occurs through heterodimer formation with a ligand-bound erbB receptor family member.
Tumor Cells, Cultured
Herpesvirus 4, Human
Nucleic Acid Conformation
Chromosomes, Artificial, Yeast
Chromosomes in which fragments of exogenous DNA ranging in length up to several hundred kilobase pairs have been cloned into yeast through ligation to vector sequences. These artificial chromosomes are used extensively in molecular biology for the construction of comprehensive genomic libraries of higher organisms.
Chromosomes, Bacterial
Exons
Carrier Proteins
Genome
Formaldehyde
A highly reactive aldehyde gas formed by oxidation or incomplete combustion of hydrocarbons. In solution, it has a wide range of uses: in the manufacture of resins and textiles, as a disinfectant, and as a laboratory fixative or preservative. Formaldehyde solution (formalin) is considered a hazardous compound, and its vapor toxic. (From Reynolds, Martindale The Extra Pharmacopoeia, 30th ed, p717)
Kidney
Chromosomes, Human, Pair 21
Nucleic Acid Amplification Techniques
Chickens
Chromosomes, Human, Pair 4
Spontaneous heterosis in larval life-history traits of hemiclonal frog hybrids. (1/2497)
European water frog hybrids Rana esculenta (Rana ridibunda x Rana lessonae) reproduce hemiclonally, transmitting only their ridibunda genome to gametes. We compared fitness-related larval life-history traits of natural R. esculenta from Poland with those of the two sympatric parental species and of newly generated F1 hybrids. Compared with either parental species, F1 hybrid offspring had higher survival, higher early growth rates, a more advanced developmental stage by day 49, and earlier metamorphosis, but similar mass at metamorphosis. R. esculenta from natural lineages had trait values intermediate between those of F1 offspring and of the two parental species. The data support earlier observations on natural R. esculenta that had faster larval growth, earlier metamorphosis, and higher resistance to hypoxic conditions compared with either parental species. Observing larval heterosis in F1 hybrids in survival, growth rate, and time to metamorphosis, however, at an even higher degree than in hybrids from natural lineages, demonstrates that heterosis is spontaneous and results from hybridity per se rather than from subsequent interclonal selection; in natural lineages the effects of hybridity and of clonal history are confounded. This is compelling evidence for spontaneous heterosis in hybrid clonals. Results on hemiclonal fish hybrids (Poeciliopsis) showed no spontaneous heterosis; thus, our frog data are not applicable to all hybrid clonals. Our data do show, however, that heterosis is an important potential source for the extensively observed ecological success of hybrid clonals. We suggest that heterosis and interclonal selection together shape fitness of natural R. esculenta lineages. (+info)Molecular characterization of two endogenous double-stranded RNAs in rice and their inheritance by interspecific hybrids. (2/2497)
We completely sequenced 13,936 nucleotides (nt) of a double-stranded RNA (dsRNA) of wild rice (W-dsRNA). A single long open reading frame (13,719 nt) containing the conserved motifs of RNA-dependent RNA polymerase and RNA helicase was located in the coding strand. The identity between entire nucleotide sequence of W-dsRNA and that of the dsRNA of temperate japonica rice (J-dsRNA, 13,952 nt) was 75.5%. A site-specific discontinuity (nick) was identified at nt 1,197 from the 5' end of the coding strand of W-dsRNA. This nick is also located at nt 1,211 from the 5' end in the coding strand of J-dsRNA. The dsRNA copy number was increased more than 10-fold in pollen grains of both rice plants. This remarkable increase may be responsible for the highly efficient transmission of J-dsRNA via pollen that we already reported. J-dsRNA and W-dsRNA were also efficiently transmitted to interspecific F1 hybrids. Seed-mediated dsRNA transmission to F2 plants was also highly efficient when the maternal parent was wild rice. The efficiency of dsRNA transmission to F2 plants was reduced when the maternal parent was temperate japonica rice; however, the reduced rates in F2 plants were returned to high levels in F3 plants. (+info)Construction and analysis of hybrid Escherichia coli-Bacillus subtilis dnaK genes. (3/2497)
The highly conserved DnaK chaperones consist of an N-terminal ATPase domain, a central substrate-binding domain, and a C-terminal domain whose function is not known. Since Bacillus subtilis dnaK was not able to complement an Escherichia coli dnaK null mutant, we performed domain element swap experiments to identify the regions responsible for this finding. It turned out that the B. subtilis DnaK protein needed approximately normal amounts of the cochaperone DnaJ to be functional in E. coli. The ATPase domain and the substrate-binding domain form a species-specific functional unit, while the C-terminal domains, although less conserved, are exchangeable. Deletion of the C-terminal domain in E. coli DnaK affected neither complementation of growth at high temperatures nor propagation of phage lambda but abolished degradation of sigma32. (+info)A novel ontogenetic pathway in hybrid embryos between species with different modes of development. (4/2497)
To investigate the bases for evolutionary changes in developmental mode, we fertilized eggs of a direct-developing sea urchin, Heliocidaris erythrogramma, with sperm from a closely related species, H. tuberculata, that undergoes indirect development via a feeding larva. The resulting hybrids completed development to form juvenile adult sea urchins. Hybrids exhibited restoration of feeding larval structures and paternal gene expression that have been lost in the evolution of the direct-developing maternal species. However, the developmental outcome of the hybrids was not a simple reversion to the paternal pluteus larval form. An unexpected result was that the ontogeny of the hybrids was distinct from either parental species. Early hybrid larvae exhibited a novel morphology similar to that of the dipleurula-type larva typical of other classes of echinoderms and considered to represent the ancestral echinoderm larval form. In the hybrid developmental program, therefore, both recent and ancient ancestral features were restored. That is, the hybrids exhibited features of the pluteus larval form that is present in both the paternal species and in the immediate common ancestor of the two species, but they also exhibited general developmental features of very distantly related echinoderms. Thus in the hybrids, the interaction of two genomes that normally encode two disparate developmental modes produces a novel but harmonious ontongeny. (+info)Introgression through rare hybridization: A genetic study of a hybrid zone between red and sika deer (genus Cervus) in Argyll, Scotland. (5/2497)
In this article we describe the structure of a hybrid zone in Argyll, Scotland, between native red deer (Cervus elaphus) and introduced Japanese sika deer (Cervus nippon), on the basis of a genetic analysis using 11 microsatellite markers and mitochondrial DNA. In contrast to the findings of a previous study of the same population, we conclude that the deer fall into two distinct genetic classes, corresponding to either a sika-like or red-like phenotype. Introgression is rare at any one locus, but where the taxa overlap up to 40% of deer carry apparently introgressed alleles. While most putative hybrids are heterozygous at only one locus, there are rare multiple heterozygotes, reflecting significant linkage disequilibrium within both sika- and red-like populations. The rate of backcrossing into the sika population is estimated as H = 0.002 per generation and into red, H = 0.001 per generation. On the basis of historical evidence that red deer entered Kintyre only recently, a diffusion model evaluated by maximum likelihood shows that sika have increased at approximately 9.2% yr-1 from low frequency and disperse at a rate of approximately 3.7 km yr-1. Introgression into the red-like population is greater in the south, while introgression into sika varies little along the transect. For both sika- and red-like populations, the degree of introgression is 30-40% of that predicted from the rates of current hybridization inferred from linkage disequilibria; however, in neither case is this statistically significant evidence for selection against introgression. (+info)A dynamically regulated 14-3-3, Slob, and Slowpoke potassium channel complex in Drosophila presynaptic nerve terminals. (6/2497)
Slob is a novel protein that binds to the carboxy-terminal domain of the Drosophila Slowpoke (dSlo) calcium-dependent potassium (K(Ca)) channel. A yeast two-hybrid screen with Slob as bait identifies the zeta isoform of 14-3-3 as a Slob-binding protein. Coimmunoprecipitation experiments from Drosophila heads and transfected cells confirm that 14-3-3 interacts with dSlo via Slob. All three proteins are colocalized presynaptically at Drosophila neuromuscular junctions. Two serine residues in Slob are required for 14-3-3 binding, and the binding is dynamically regulated in Drosophila by calcium/calmodulin-dependent kinase II (CaMKII) phosphorylation. 14-3-3 coexpression dramatically alters dSlo channel properties when wild-type Slob is present but not when a double serine mutant Slob that is incapable of binding 14-3-3 is present. The results provide evidence for a dSlo/Slob/14-3-3 regulatory protein complex. (+info)Genetics of graft-versus-host disease, I. A locus on chromosome 1 influences development of acute graft-versus-host disease in a major histocompatibility complex mismatched murine model. (7/2497)
Graft-versus-host disease (GVHD) is the major complication occurring after bone marrow transplantation. The severity of GVHD varies widely, with this variation generally being attributed to variation in the degree of disparity between host and donor for minor histocompatibility antigens. However, it is also possible that other forms of polymorphism, such as polymorphisms in immune effector molecules, might play a significant role in determining GVHD severity. In order to investigate this hypothesis, we are studying the genetic factors that influence GVHD development in a murine model. We here report the first results of this analysis, which demonstrate that a locus on Chromosome 1 of the mouse, and possibly also a locus on Chromosome 4, exert considerable influence over the development of one aspect of acute GVHD - splenomegaly - in a parent-->F1 murine model. These results demonstrate that non-MHC genes can exert quite significant effects on the development of GVHD-associated pathology and that gene mapping can be used as a tool to identify these loci. Further analysis of such loci will allow identification of the mechanism whereby they influence GVHD and may lead in the future to improved selection of donors for human bone marrow transplantation. (+info)Suppressor T-cell activity in responder X nonresponder (C57BL/10 X DBA/1)F1 spleen cells responsive to L-glutamic acid60-L-alanine30-L-tyrosine10. (8/2497)
The ability of spleen cells from (responder X nonresponder)F(1) mice immunized with various GAT-Mphi, GAT-MBSA, and soluble GAT to develop IgG GAT-specific PFC responses in vitro after stimulation with responder and nonresponder parental and F(1) GAT-Mphi, was investigated. F(1) spleen cells from mice immunized with F(1) GAT-Mphi or GAT-MBSA developed secondary responses to responder and nonresponder parental and F(1) GAT- Mphi, but not to unrelated third party GAT-Mphi. Spleen cells from F(1) mice immunized with either parental GAT-Mphi developed secondary responses to F(1) GAT-Mphi and only the parental GAT-Mphi used for immunization in vivo. Soluble GAT-primed F(1) spleen cells responded to F(1) and responder parental, but not nonresponder parental, GAT-Mphi. Simultaneous immunization in vivo with the various GAT-Mphi or GAT-MBSA plus soluble GAT modulated the response pattern of these F(1) spleen cells such that they developed secondary responses only to F(1) and parental responder GAT-Mphi regardless of the response pattern observed after immunization with the various GAT-Mphi or GAT-MBSA alone. These observations demonstrate the critical importance of the physical state of the GAT used for immunization in determining the subsequent response pattern of immune F(1) spleen cells to the parental and F(1) GAT-Mphi. Further, suppressor T cells, capable of inhibiting primary responses to GAT by virgin F(1) spleen cells stimulated by nonresponder parental GAT-Mphi, were demonstrated in spleens of F(1) mice immunized with soluble GAT, but not those primed with F(1) GAT-Mphi. Because responder parental mice develop both helper and suppressor T cells after immunization with GAT-Mphi, and soluble GAT preferentially stimulates suppressor T cells whereas GAT-Mphi stimulate helper T cells in nonresponder parental mice, these observations suggest that distinct subsets of T cells exist in F(1) mice which behave phenotypically as responder and nonresponder parental T cells after immunization with soluble GAT and GAT- Mphi. (+info)
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Get PDF - Inheritance in Nicotiana tabacum XXIX: The relationships of residual chromosome homology to interspecific gene...
Understanding the extent of interspecific hybridization and exactly how ecological segregation
Experimental hybridization and backcrossing reveal forces of reproductive isolation in Microbotryum | BMC Evolutionary Biology ...
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New hybrid count- and geometry-based method for quantification of left ventricular volumes and ejection fraction from ECG-gated...
Symmetric interspecies hybrids of mouse and human hemoglobin: Molecular basis of their abnormal oxygen affinity<...
A quantitative genetic model for mixed diploid and triploid hybrid progenies in tree breeding and evolution<...
Eco-ethological aspects of two natural hybrids involving Abramis brama (L.), of the River Meuse basin (Belgium) and...
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Introgression
... , also known as introgressive hybridization, in genetics is the transfer of genetic material from one species into ... Chimera (genetics) Genetic engineering Genetic erosion Genetic pollution Transgene Transgenic plant Anderson, Edgar; Hubricht, ... Introgression or introgressive hybridization is the incorporation (usually via hybridization and backcrossing) of novel genes ... Introgressive hybridization. New York: Wiley & Sons Harrison, R (2014). "Hybridization, Introgression, and the Nature of ...
Frederick Parker-Rhodes
A case of hybridization in Psilocybe (Deconica)". New Phytologist 49 (3): 335-343. (subscription required) ________________. ( ... Genetic isolation in Panaeolus papilionaceous". New Phytologist 49: 328-334. ________________. (1950). "The Basidiomycetes of ...
Pinus ponderosa
Conkle, MT; Critchfield, WB (1988). "Genetic variation and hybridization of ponderosa pine". In Baumgartner, DM; Lotan, JE (eds ... Van Haverbeke, DF (1986). Genetic variation in ponderosa pine: A 15-Year Test of provenances in the Great Plains. USDA Forest ... ISBN 90-04-13916-8. Haller, JR (1962). Variation and hybridization in ponderosa and Jeffrey pines. University of California ...
Annona reticulata
It is also a genetic resource for hybridization. The leaves and the branches can be used for tanning as they contain blue ...
Solanum tuberosum Group Phureja
By hybridization with Solanum tuberosum by the Scottish Crop Research Institute, varieties were obtained who are adapted to the ... Dodds, Kenneth S.; Paxman, G. J. (1962). "The Genetic System of Cultivated Diploid Potatoes". Evolution. 16 (2): 154. doi: ... Hybridization of Crop Plants.. Nueva York: American Society of Agronomy, Crop Science Society of America. pp. 483-494. ISBN 0- ... 89118-034-6. ↑ Hawkes, J.G. (1990). The potato: evolution, biodiversity and genetic resources.. Londres: Belhaven Press,. pp. ...
Leopold Kirschner
"Genetic characterization of pathogenic Leptospira species by DNA hybridization". International Journal of Systematic ...
Pallid sturgeon
Tranah, G. J.; D. E. Campton; B. May (2004). "Genetic Evidence for Hybridization of Pallid and Shovelnose Sturgeon". Journal of ... However, the genetic variability among pallid sturgeon was found to be far less than that between them and the shovelnose ... Hybrids are most common in the Atchafalaya River in Louisiana, and DNA sequencing in these hybrids showed a genetic distinction ... Another reason for DNA testing was to determine the rates of hybridization between pallid and shovelnose sturgeon. The southern ...
Leptospira kirschneri
"Genetic characterization of pathogenic Leptospira species by DNA hybridization". International Journal of Systematic ...
Gulf menhaden
Genetic evidence for asymmetric hybridization between menhadens (Brevoortia spp.) from peninsular Florida. Journal of Fish ... Morphological and genetic investigations of two western Gulf of Mexico menhadens (Brevoortia spp.). Journal of Fish Biology 70a ... and hybridization between these species has been demonstrated using morphological and DNA evidence. Gulf menhaden also may have ... and populations of Gulf menhaden throughout the Gulf of Mexico are generally thought to comprise a single genetic stock. The ...
Rhesus macaque
"Anthropogenic macaque hybridization and genetic pollution of a threatened population". 7 (1). Tropical Natural History: 11-23 ... pet releases of the different species into existing troops are diluting the gene pool and putting its genetic integrity at risk ...
Bernard Dujon
Morales, Lucia; Dujon, Bernard (December 2012). "Evolutionary Role of Interspecies Hybridization and Genetic Exchanges in ... Bernard Dujon decided to adapt the mitochondrial gene to the universal genetic code in order to be able to express it in a ... At that time, no one had any idea of the genetic content of mitochondria, except that it contained DNA. Bernard Dujon was ... In Gif-sur-Yvette, Bernard Dujon started to study a strange genetic phenomenon, linked to mitochondrial genetics, whose study ...
Cultural amalgamation
This in turn creates a genetic mergence through the birth of children. This genetic process, also known as hybridization, ... Bourdieu describes hybridization as a much more subtle, hidden or disguised form for powerful material contributions in ... "Hybridization". Genome.gov. Retrieved 2022-04-07. . (CS1 errors: missing periodical, Cultural concepts, Culture). ... Procreating between two individuals from two isolated and different cultures creates a hybridization state in the resulting ...
Nagendra Kumar Singh
for BAC DNA fingerprinting, genetic mapping and BAC filter hybridization 2. Cloning Lab, used for shotgun cloning of BAC clones ... The structure and genetic control of a new class of disulphide-linked proteins in wheat endosperm. Theoretical and Applied ... "Structure and Genetic control of endosperm proteins in wheat and rye". There he was awarded Dr K.P. Barley Prize best post ...
Canada lynx
Schwartz, M. K. (2004). "Hybridization between Canada lynx and bobcats: genetic results and management implications". ... Hybridization between Canada lynxes and bobcats has been reported in the southern periphery of the range. Hybridization between ... Rueness, E. K.; Stenseth, N. C.; O'Donoghue, M.; Boutin, S.; Ellegren, H. & Jakobsen, K. S. (2003). "Ecological and genetic ... Schwartz, M. K.; Mills, L. S.; Ortega, Y.; Ruggiero, L. F.; Allendorf, F. W. (2003). "Landscape location affects genetic ...
Red wolf
"Genetic evidence for a recent origin by hybridization of red wolves". Molecular Ecology. 8 (1): 139-144. doi:10.1046/j.1365- ... eastern wolf hybridization, eastern coyotes are the result of eastern wolf × western coyote hybridization, and red wolves are ... the hybridization could not have occurred recently but supports a much more ancient hybridization. The group found deficiencies ... Genetic data supports a close relationship between the eastern and red wolves, but not close enough to support these as one ...
Tufted titmouse
Tufted titmice will occasionally hybridize with the black-crested titmouse; the hybridization range is very narrow, however, ... due to genetic differences. Tufted Titmouse (Tuftie) with a bit of snow on her beak. Tufted Titmouse facing the camera, Tufted ...
Patellifolia
As a result, they show only weak genetic barriers to hybridization. Patellifolia is a rather old genus; the divergence from its ... But recent molecular genetic studies by Kadereit et al. (2006) and Romeiras et al. (2016) revealed a deep genetic ... The island species evolved recently, and because of their isolation they developed only small genetic differentiation. ...
Dianthus acicularis
Mii, M. (2012). "Ornamental Plant Breeding Through Interspecific Hybridization, Somatic Hybridization and Genetic ...
Italian wall lizard
Capula M (2002). "Genetic evidence of natural hybridization between Podarcis sicula and Podarcis tiliguerta (Reptilia)". ... There have also been reports of hybridization between P. siculus and other species of the Podarcis genus, such as P. tilguerta ...
Rhus michauxii
2002). Genetic Variation and Evidence of Hybridization in the Genus Rhus (Anacardiaceae). Journal of Heredity. 93(1) 37-41. ... Conservation activities include genetic analysis in an effort to understand the genetic variability of populations. Plant ... It is clonal, often reproducing vegetatively, so populations are low in genetic variability. It may hybridize with the common ...
Cleavage (embryo)
"Preimplantation genetic diagnosis and chromosome analysis of blastomeres using comparative genomic hybridization". Human ... A single cell can be removed from a pre-compaction eight-cell embryo and used for genetic screening, and the embryo will ... Kim HJ, Kim CH, Lee SM, Choe SA, Lee JY, Jee BC, Hwang D, Kim KC (September 2012). "Outcomes of preimplantation genetic ...
Falkland steamer duck
Possible hybridization could account for the genetic similarities between the two species. Some scientists have proposed that ... In fact, both the Falkland steamer duck and the closely-related flying steamer duck contain genetic sequences associated with ... Lele, Abhimanyu; Ottenburghs, Jente (2019). "Digest: A single genetic origin and a role for bone development pathways in ... Colihueque, Nelson; Gantz, Alberto (2019-11-28). "Molecular genetic studies of Chilean avifauna: an overview about current ...
Rhinella pombali
"Delimiting genetic units in Neotropical toads under incomplete lineage sorting and hybridization". BMC Evolutionary Biology. 12 ... It was originally described in 2004 as a distinct species, but a genetic study published in 2012 found it to be a hybrid of ...
Koshu (grape)
"Genetic Analysis of East Asian Grape Cultivars Suggests Hybridization with Wild Vitis". Nami Goto-Yamamoto, Jason Sawler, Sean ...
TM7x
Fluorescent in situ hybridization (FISH) can be used to observe cell separation. and single cells can be acquired using flow ... Genetic approaches, such as complete DNA sequencing or whole genome sequencing. and 16S RNA sequencing. define the microbiome, ... to conduct genetic analysis. These methods, in conjunction with well-established sample preparation, staining techniques and co ... July 2007). "Dissecting biological "dark matter" with single-cell genetic analysis of rare and uncultivated TM7 microbes from ...
Rhinella crucifer
"Delimiting genetic units in Neotropical toads under incomplete lineage sorting and hybridization". BMC Evolutionary Biology. 12 ...
Tremblay's salamander
This hybridization created two all-female species: Tremblay's and silvery salamanders. These genetic curiosities possess three ... The males' chromosome contribution only stimulates the egg's development; its genetic material is ignored. It is not often ...
Joyce Van Eck
"Professor Joyce Van Eck - Improving Crops By Genetic Engineering And Targeted Genome Editing • scientia.global". February 15, ... "Transfer of large amounts of DNA via somatic hybridization and particle bombardment". hdl:2027/coo.31924062830223 - via ...
Vivipary
... hybridization and genetic mosaicism in Posidonia australis(Posidoniaceae)". Annals of Botany. 117 (2): 237-47. doi:10.1093/aob/ ...
Bolivian river dolphin
"Living between Rapids: Genetic Structure and Hybridization in Botos ( Cetacea: Iniidae: Inia Spp.) of the Madeira River, Brazil ... Preserving habitats is important for species richness, genetic diversity, and ecosystem complexity. Bolivian river dolphins are ...
Almond
The genetic basis for bitterness involves a single gene, the bitter flavor furthermore being recessive,[17][18] both aspects ... However, through natural hybridisation between different almond varieties, a new variety that was self-pollinating with a high ... G. Ladizinsky (1999). "On the origin of almond". Genetic Resources and Crop Evolution. 46 (2): 143-147. doi:10.1023/A: ... "Use of recessive homozygous genotypes to assess genetic control of kernel bitterness in almond". Euphytica. Springer. 153 (1-2 ...
Giant trevally
Initial evidence of hybridisation was morphological characteristics intermediate to the two species; later genetic tests ... which was considered to be the reason for hybridisation.[52] ...
Broiler
Recent genetic analysis has revealed that the gene for yellow skin was incorporated into domestic birds through hybridization ...
Sweet pea
Many genetic principles were discovered or confirmed in this species. It was used by Punnett in early studies of genetic ... Hybridization with Lathyrus belinensis[edit]. Like the blue rose, the yellow sweet pea remains elusive. Lathyrus belinensis is ... It is highly suitable as a genetic subject because of its ability to self-pollinate and its easily observed Mendelian traits ...
Oncogenomics
Array Comparative Genome Hybridization: This technique measures the DNA copy number differences between normal and cancer ... Before BRAF, the genetic mechanism of melanoma development was unknown and therefore prognosis for patients was poor.[71] ... Cooper DN, Youssoufian H (February 1988). "The CpG dinucleotide and human genetic disease". Hum. Genet. 78 (2): 151-5. doi: ... Cancer is a genetic disease caused by accumulation of DNA mutations and epigenetic alterations leading to unrestrained cell ...
Thymus
Autoimmune polyendocrine syndrome type 1, is a rare genetic autoimmune syndrome that results from a genetic defect of the ... The condition is diagnosed by fluorescent in situ hybridization and treated with thymus transplantation.[15] ... Immunodeficiency can be profound.[9] Loss of the thymus at an early age through genetic mutation (as in DiGeorge syndrome, ... Genetic analysis including karyotyping may reveal specific abnormalities that may influence prognosis or treatment, such as the ...
Kit fox
... but this hybridization is quite restricted in scope.[3] ... "Genetic Subdivisions among Small Canids: Mitochondrial DNA ...
Neoplasm
These cells are presumed to be monoclonal - that is, they are derived from the same cell,[10] and all carry the same genetic or ... Tumors in humans occur as a result of accumulated genetic and epigenetic alterations within single cells, which cause the cell ... which can be caused by genetic mutations. Not all types of neoplasms cause a tumorous overgrowth of tissue, however (such as ... "Genetic reconstruction of individual colorectal tumor histories". Proc. Natl. Acad. Sci. U.S.A. 97 (3): 1236-41. Bibcode ...
Common buzzard
a b c Kruckenhauser, L., Haring, E., Pinsker, W., Riesing, M. J., Winkler, H., Wink, M., & Gamauf, A. (2004). Genetic vs. ... Elorriaga, J., & Muñoz, A. R. (2013). Hybridisation between the Common Buzzard Buteo buteo buteo and the North African race of ... However, the distinctiveness of these African buzzards has generally been supported.[11][12][13] Genetic studies have further ... first results of a genetic analysis based on nucleotide sequences of the cytochrome b gene. Alauda, 68(1), 55-58. ...
Bottlenose dolphin
Some recent genetic evidence suggests the Indo-Pacific bottlenose dolphin belongs in the genus Stenella, since it is more like ... such as past hybridization between Stenella and ancestral Tursiops) and incomplete lineage sorting, and thus support T. ... In general, genetic variation between populations is significant, even among nearby populations.[22] As a result of this ... Charlton-Robb, K.; Taylor, A. C.; McKechnie, S. W. (February 2015). "Population genetic structure of the Burrunan dolphin ( ...
जीव विज्ञान - विकिपीडिया
1866 ग्रेगर मेंडल - पादपों में संकरण (hybridization) के प्रयोगों के बारे में पहला प्रकाशन, आनुवंशिकी की स्थापना ... जैवप्रौद्योगिकी (Biotechnology) - the study of the manipulation of living matter, including genetic modification and synthetic ...
Spruce
Douglas, G.W. (1975). Spruce (Picea) hybridization in west-central British Columbia. B.C. Min. For., Forest Science, Smithers ... genetic composition of the seeds produced by a stand is determined by both the seed trees and the pollen parents, and species ... Species classification for seeds collected from spruce stands in which introgressive hybridization between white and Sitka ... Species classification of seedlots collected in areas where hybridization of white and Sitka spruces has been reported has ...
హోమో - వికీపీడియా
Evidence from Studies of Interspecific Hybridization". Proceedings of the National Academy of Sciences (in ఇంగ్లీష్). 71 (7): ... Reich, D; Green, RE; Kircher, M (December 2010). "(December 2010). "Genetic history of an archaic hominin group from Denisova ... This study raises the possibility of observed genetic affinities between archaic and modern human populations being mostly due ... Genetic evidence has been adduced for an age of roughly 270,000 years.[89] ...
Hormon rasta oslobađajući hormonski receptor
Maheshwari HG, Silverman BL, Dupuis J, Baumann G (1998). „Phenotype and genetic analysis of a syndrome caused by an ... to 7p14 by in situ hybridization.". Genomics 19 (1): 193-5. DOI:10.1006/geno.1994.1045. PMID 8188233. ...
படிவளர்ச்சிக் கொள்கை - தமிழ் விக்கிப்பீடியா
Wright, S (1984). Evolution and the Genetics of Populations, Volume 1: Genetic and Biometric Foundations. The University of ... "Experiments in Plant Hybridization".. .mw-parser-output cite.citation{font-style:inherit}.mw-parser-output .citation q{quotes ... இதற்குத் தகவமைவு அல்லது மரபணு பிறழ்வு நகர்ச்சி (Genetic drift) என்று பெயர். இக்கொள்கை புவியிலுள்ள உயிர்களின் பொது ...
Egg
Lokki, J., Suomalainen, E., Saura, A, & Lankinen, P. (Mars 1975). «Genetic Polymorphism and Evolution in Parthenogenetic ... Cuellar, O; McKinney, CO (juni 1976). «Natural hybridization between parthenogenetic and bisexual lizards: detection of ...
Land snail
There have been hybridizations of snail species; although these do not occur commonly in the wild, in captivity they can be ... Selander, R. K.; Ochman, H. (1983). "The genetic structure of populations as illustrated by molluscs". In Rattazzi, M. C.; ...
Invasive species
Genetic pollution is unintentional hybridization and introgression, which leads to homogenization or replacement of local ... Genetic Pollution from Farm Forestry using eucalypt species and hybrids; A report for the RIRDC/L&WA/FWPRDC]; Joint Venture ... Harmful effects of hybridization have led to a decline and even extinction of native species.[92][93] For example, ... For example, the bee Lasioglossum leucozonium, shown by population genetic analysis to be an invasive species in North America, ...
Deletion (genetics)
In genetics, a deletion (also called gene deletion, deficiency, or deletion mutation) (sign: Δ) is a mutation (a genetic ... In particular, microarray-comparative genomic hybridization (CGH) based on the use of BAC clones promises a sensitive strategy ... Deletions are responsible for an array of genetic disorders, including some cases of male infertility, two thirds of cases of ... Deletions can be caused by errors in chromosomal crossover during meiosis, which causes several serious genetic diseases. ...
Reverse transcription polymerase chain reaction
Genetic disease diagnosisEdit. RT-PCR can be used to diagnose genetic disease such as Lesch-Nyhan syndrome. This genetic ... "Method for detection of specific RNAs in agarose gels by transfer to diazobenzyloxymethyl-paper and hybridization with DNA ... RT-PCR is widely used in the diagnosis of genetic diseases and, semiquantitatively, in the determination of the abundance of ...
Black stork
... genetic analysis via DNA-DNA hybridization and mitochondrial cytochrome b DNA by Beth Slikas in 1997 found that it was basal ( ... Slikas, Beth (1997). "Phylogeny of the Avian Family Ciconiidae (Storks) Based on CytochromebSequences and DNA-DNA Hybridization ...
Proto-oncogene tyrosine-protein kinase Src
A common mechanism is that there are genetic mutations that result in the increased activity or the overexpression of the c-Src ... "Detection and enumeration of transformation-defective strains of avian sarcoma virus with molecular hybridization". Virology. ...
Genomic imprinting
The first imprinted genetic disorders to be described in humans were the reciprocally inherited Prader-Willi syndrome and ... that these imprinted genes are responsible for the triploid block effect in flowering plants that prevents hybridization ... A hypothesis for the origin of this genetic variation states that the host-defense system responsible for silencing foreign DNA ... It is an epigenetic process that involves DNA methylation and histone methylation without altering the genetic sequence. These ...
Indian python
A genetic study published in 2017 showed that the Burmese pythons in Florida are hybrids with P. molurus. In the literature, ... population reveals possible hybridization with the Indian python (P. molurus)". Ecology and Evolution. 8 (17): 9034-9047. doi: ...
Liquidambar acalycina
Hoey, Margaret; Parks, Clifford (1994). "Genetic Divergence in Liquidambar styraciflua, L. formosana, and L. acalycina". ... "Molecular evidence for natural intergeneric hybridization between Liquidambar and Altingia". Journal of Plant Research. 123 (2 ...
MiR-137
In particular, in situ hybridisation showed an enhanced expression of miR-137 within the dentate gyrus and the molecular layer ... Cross-Disorder Group of the Psychiatric Genomics Consortium; Genetic Risk Outcome of Psychosis (GROUP) Consortium (2013). " ...
Prunus × nudiflora
natural hybridization either in the Izu peninsula, on Izu-oshima Island or on Cheju-do Island in Korea, although the ... June 1998). "Genetic relationship of Prunus yedoensis, native and cultivar, based on internal transcribed spacer sequences of ... 2018). "Draft genome sequence of wild Prunus yedoensis reveals massive inter-specific hybridization between sympatric flowering ... 2017). "Chloroplast Noncoding DNA Sequences Reveal Genetic Distinction and Diversity between Wild and Cultivated Prunus ...
Cell cycle
Many of the relevant genes were first identified by studying yeast, especially Saccharomyces cerevisiae; genetic nomenclature ... "Comprehensive identification of cell cycle-regulated genes of the yeast Saccharomyces cerevisiae by microarray hybridization". ... including the detection and repair of genetic damage as well as the prevention of uncontrolled cell division. The molecular ...
Timeline of human evolution
Patterson N, Richter DJ, Gnerre S, Lander ES, Reich D (2006). "Genetic evidence for complex speciation of humans and ... "Mitochondrial pseudogenes suggest repeated inter-species hybridization in hominid evolution". bioRxiv: 134502. doi:10.1101/ ... Callaway, Ewen (26 July 2012). "Hunter-gatherer genomes a trove of genetic diversity". Nature. doi:10.1038/nature.2012.11076. ... Stein, Richard A. (October 2015). "Copy Number Analysis Starts to Add Up". Genetic Engineering & Biotechnology News. 35 (17): ...
Short interspersed nuclear element
The activity of SINEs however has genetic vestiges which do not seem to play a significant role, positive or negative, and ... The genes that had high hybridization E-values were genes particularly involved in metabolic and signaling pathways. Almost all ... The distribution of SINEs to genes was significantly more similar than that of other non-coding genetic elements and even ... SINEs are also implicated in certain types of genetic disease in humans and other eukaryotes. In essence, short interspersed ...
Fluorescence in situ hybridization in combination with the comet assay and micronucleus test in genetic toxicology
Dryad | Data -- Long-term persisting hybrid swarm and geographic difference in hybridization pattern: genetic consequences of...
Data from: Long-term persisting hybrid swarm and geographic difference in hybridization pattern: genetic consequences of ... Long-term persisting hybrid swarm and geographic difference in hybridization pattern: genetic consequences of secondary contact ... and assessed the genetic consequences of secondary contact by combining morphological and genetic approaches. We performed ... Pattern of hybridization between V. atratum and V. japonicum is unidirectional and differs among populations. We concluded that ...
Multilocus genetic analyses provide insight into speciation and hybridization in aquatic grasses, genus Ruppia | - CCMAR
Urine Tumor Markers in Bladder Cancer Diagnosis: Overview of Urine Tumor Markers, Genetic Aberrations in Bladder Cancer,...
Commercially available tests include the following: Urine cytology Fluorescence in situ hybridization (FISH) Nuclear matrix ... Genetic Aberrations in Bladder Cancer. The study of genetic aberrations commonly associated with urothelial carcinoma provides ... Homozygous loss of band 9p21, the site for the tumor suppressor gene P16, is a known early genetic event in the development of ... Fluorescence In Situ Hybridization. A commercial FISH assay (UroVysion Bladder Cancer Kit, Abbott Molecular; DesPlaines, Ill), ...
العربية
Distribution of DYZ2 repetitive sequences on the human Y chromosome
In this work, we have performed non-radioactive in situ hybridization of a cloned DYZ2 fragment at higher stringency … ... In this work, we have performed non-radioactive in situ hybridization of a cloned DYZ2 fragment at higher stringency conditions ... Genetic Techniques * Heterochromatin * Humans * In Situ Hybridization * Male * Multigene Family* * Repetitive Sequences, ...
Recommendations for the Laboratory-Based Detection of Chlamydia trachomatis and Neisseria gonorrhoeae - 2014
Nucleic acid hybridization/probe tests. Two nucleic acid hybridization assays are FDA-cleared to detect C. trachomatis or N. ... Genetic mutations and/or acquisition of genetic material from closely related bacteria species might result in antibiotic- ... Nucleic acid genetic transformation tests. The Gonostat test (Sierra Diagnostics, Incorporated, Sonora, California) uses a ... Genetic transformation as a tool for detection of Neisseria gonorrhoeae. J Clin Microbiol 1976;4:71-81. ...
fluorescence in situ hybridization Archives : Inside Children's Blog
Genetic, morphological, and chemical patterns of plant hybridization | Revista Chilena de Historia Natural | Full Text
Hybridization is considered an important evolutionary force since it may lead to (1) an increase of the intraspecific genetic ... In this review, we give a general historical background of the study of plant hybridization. Also, we review some of the tools ... Because of the importance of plant hybridization in evolution, it is of great importance to accurately identify hybrid ... This is of special importance when hybridization leads to evolutionary novelty in the form of polyploidy, transgressive ...
Molecular genetic testing and the future of clinical genomics | Nature Reviews Genetics
Genomic technologies are reaching the point of being able to detect genetic variation in patients at high accuracy and reduced ... In parallel, we outline implementation challenges that will be necessary to address to ensure the future of genetic medicine. ... The authors review current technologies for clinical genetic testing. Moves are being made towards whole-genome and whole-exome ... Hybridization of a nucleic acid sample to a very large set of oligonucleotide probes, which are attached to a solid support, to ...
Hybridization between Calopteryx splendens and C. haemorrhoidalis confirmed by morphological and genetic analyses - Worldwide...
Hybridization between Calopteryx splendens and C. haemorrhoidalis confirmed by morphological and genetic analyses M. Olalla ... Hybridization between Calopteryx splendens and C. haemorrhoidalis confirmed by morphological and genetic analyses. ... Hybridization between Calopteryx splendens and C. haemorrhoidalis confirmed by morphological and genetic analyses ... Hybridization between Calopteryx haemorrhoidalis and any of its congeners has not been reported until now. We observed ...
Myelodysplastic syndrome: MedlinePlus Medical Encyclopedia
Cytogenetics and fluorescent in situ hybridization (FISH) are used for genetic analysis. Cytogenetic testing can detect ... Genetic variations may help determine response to treatment.. Some of these tests will help your provider determine what type ... Certain genetic disorders. *Exposure to environmental or industrial chemicals, fertilizers, pesticides, solvents, or heavy ... translocations and other genetic abnormalities. FISH is used to identify specific changes within chromosomes. ...
تصفح EMRO Journal Articles (EMHJ) حسب العنوان
A genetic epidemiological study of malformations at birth in Egypt Temtamy, S.A. (1998) A total of 3000 consecutive ... Cytogenetic studies included fluorescence in situ hybridization [FISH] when indicated. In all, 15 patients had chromosomal ... A diagnostic clinical genetic study of craniofacial dysmorphism Farag, H.M. (1999) A diagnostic evaluation of craniofacial ... Complete genetic examination, pedigree analysis, anthropometric measurements and radiological studies were carried out. ...
Direct fluorescence analysis of genetic polymorphisms by hybridization with oligonucleotide arrays on glass supports | Smith...
Direct fluorescence analysis of genetic polymorphisms by hybridization with oligonucleotide arrays on glass supports. *Posted ... Home » Direct fluorescence analysis of genetic polymorphisms by hybridization with oligonucleotide arrays on glass supports. ... Direct fluorescence analysis of genetic polymorphisms by hybridization with oligonucleotide arrays on glass supports. ...
NIOSHTIC-2 Search Results - Full View
Sweet pea - Wikipedia
Many genetic principles were discovered or confirmed in this species. It was used by Punnett in early studies of genetic ... Hybridization with Lathyrus belinensis[edit]. Like the blue rose, the yellow sweet pea remains elusive. Lathyrus belinensis is ... It is highly suitable as a genetic subject because of its ability to self-pollinate and its easily observed Mendelian traits ...
Talking Glossary of Genetic Terms | NHGRI
In Situ Hybridization. In situ hybridization is a laboratory technique used to localize a sequence of DNA or RNA in a ... Genetic Map. A genetic map (also called a linkage map) shows the relative location of genetic markers (reflecting sites of ... Genetic Epidemiology. Genetic epidemiology is a field of science focused on the study of how genetic factors influence human ... Genetic Counseling. Genetic counseling refers to guidance relating to genetic disorders that a specialized healthcare ...
Genetic and physical maps of the Primula vulgaris S locus and localization by chromosome in situ hybridization - Molecular...
Integration of genetic and physical maps of the Primula vulgaris S locus and localization by chromosome in situ hybridization. ... Genetic and physical maps of the Primula vulgaris S locus and localization by chromosome in situ hybridization. Posted by Pat ... Integration of genetic and physical maps of the Primula vulgaris S locus and localization by chromosome in situ hybridization. ... food security genes genetic resources genetics genome genome evolution genomes genomics germplasm heslop harrison hybridization ...
The evolution of spring fen ecotypes in Rhinanthus: genetic evidence for parallel origins in Scandinavia after the last ice age...
Ducarme V, Wesselingh RA (2005) Detecting hybridization in mixed populations of Rhinanthus minor and Rhinanthus angustifolius. ... Genetic diversity within populations. The genetic diversity of individual populations was estimated as the number of alleles (A ... 2000) to explore the genetic structuring of populations from Gotland only, and to explore the genetic structuring of the ... Peakall R, Smouse PE (2012) GenAlEx 6.5: genetic analysis in Excel. Population genetic software for teaching and research-an ...
Amniochip Detects 150 Genetic Syndromes from Amniotic Fluid
... has developed a chip that is capable of detecting 150 different genetic syndromes from a few milliliters of amniotic ... It uses comparative genomic hybridization micro-array technology to identify areas with differences in hybridization between ... Amniochip Detects 150 Genetic Syndromes from Amniotic Fluid. April 11th, 2011 Wouter Stomp Genetics, Pathology ... Genetadi Biotech (Derio, Spain) has developed a chip that is capable of detecting 150 different genetic syndromes from a few ...
Defective Repair Replication of DNA in Xeroderma Pigmentosum | Semantic Scholar
Genetic heterogeneity of xeroderma pigmentosum demonstrated by somatic cell hybridization.. *E. A. de Weerd-Kastelein, W. ... Genetic Analysis of DNA Repair Defect in Xeroderma Pigmentosum Cells: Identification of Complementing Genes. *G. P. Kaur, R. ... DNA repair and genetic recombination: studies on mutants of Escherichia coli defective in these processes.. *P. Howard-Flanders ... including alkylating agents and X-rays are reviewed and the role of DNA Repair in genetic recombination is reviewed.. Expand. ...
Hybridisation and introgression between Brassica napus and Brassica rapa in the Netherlands
Scientists peel back ancient layers of banana DNA - The Jerusalem Post
... specifically hybridization and a selection for heterozygotes, as mechanisms for the transition from wild to cultivated bananas ... The study identified a history of genetic engineering, ... "Breeders need to understand the genetic make-up of todays ... The study identified a history of genetic engineering, specifically hybridization and a selection for heterozygotes, as ... The study identified a history of genetic engineering, specifically hybridization and a selection for heterozygotes, as ...
Thank Plate Tectonics for Tasty Oranges | Discover Magazine
Grapefruit was created through hybridization, not a genetic mutation. *genetics. 1. free article. left ... Within this new genetic study, scientists now have a catalog of genes for more than 60 kinds of citrus. This database could ... A new and sweeping genetic study, published Wednesday in Nature, has pinpointed the origins and evolution of citrus. The study ... First-Ever Genetic Analysis Of A Neanderthal Family Paints A Fascinating Picture Of A Close-Knit Community ...
Methicillin-Susceptible, Vancomycin-Resistant Staphylococcus aureus, Brazil - Volume 21, Number 10-October 2015 - Emerging...
VS-MSSA and VR-MSSA have the characteristic CC5 genetic traits described by Kos et al. (8). The genome of VR-MSSA has a ... Plasmids carrying the vanA gene cluster were detected by using S1 nuclease digestion followed by hybridization with a vanA ... MRSA USA300 genetic lineage that has rapidly disseminated in the United States and the northern region of South America (USA300 ... which suggested a close genetic relationship and probably representing the same organism that acquired pBRZ01. Phylogenetic ...
Source: 2009 v.166 no.3 / Subject: 4 selected / Subject term: Brazil - PubAg Search Results
Publication : USDA ARS
In this study, genetic variability among isolates was determined by DNA hybridization analysis of restriction endonuclease (RE ... Seven distinct genetic groups were detected. A previous report has shown that sphB contains a region of 45 base pair direct ... Hybridization was performed using digests of over 200 different isolates from different geographic regions. ... Technical Abstract: It was previously found that genetic variability among Leptospira borgpetersenii serovar hardjo type ...
Situ HybridizationFluorescentGenomeRole of hybridizationPolymerase chain reIntrogressionInterspecificSpeciesGenomicSpeciationGenesMutationsChromosomePhenotypicProbeDetectionHybridsDiagnosisPhylogeographyCytogeneticPopulation geneticsDiploidAmplificationVariationVirulenceAnalysisMutationPulsed-field gel electPopulationsPhenotypeHeterogeneityAnalysesDiversityGeographicBiotechPlantFISHFetal2021LineageDifferencesPatternsArrayAnomaliesChromosomesAutosomal recessiveCharacterizationDisordersOutcomes
Situ Hybridization22
- The potential of these two methods can be enhanced by the combination with fluorescence in situ hybridization (FISH) techniques. (nih.gov)
- Cytogenetics and fluorescent in situ hybridization (FISH) are used for genetic analysis. (medlineplus.gov)
- Cytogenetic studies included fluorescence in situ hybridization [FISH] when indicated. (who.int)
- We have characterized this region by Illumina sequencing and bioinformatic analysis, together with chromosome in situ hybridization. (molcyt.org)
- Fluorescence in situ hybridization (FISH) looks for extra copies of the HER2 gene. (healthline.com)
- Cytogenetic studies, fluorescent in situ hybridization, and polymerase chain reaction tests detect genetic and chromosomal changes in cells. (medicalnewstoday.com)
- The global in-situ hybridization market size was USD 14.32 Billion in 2021 and is expected to register a revenue CAGR of 8.14% during the forecast period. (emergenresearch.com)
- Rising prevalence of cancer, genetic disorders, and infectious diseases and increasing usage of in-situ hybridization techniques for visualizing and quantifying viral load are major factors driving market revenue growth. (emergenresearch.com)
- In addition, increasing number of research initiatives to enhance sensitivity of conventional ISH and growing usage of in-situ hybridization in combination with immunohistochemistry for protein detection are some other factors expected to contribute to revenue growth of the market between 2022 and 2030. (emergenresearch.com)
- In-Situ Hybridization (ISH) is a molecular biology technique for detection and localization of nucleic acids in cells, histological sections, and other biological samples. (emergenresearch.com)
- Increasing demand for in-situ hybridization can also be attributed to rising prevalence of cancer. (emergenresearch.com)
- Fluorescence in-situ hybridization enables mapping of genome of individuals using fluorophores, which can be used by oncology researchers to determine extra copies of DNA regions and genes. (emergenresearch.com)
- COVID-19 pandemic has significantly driven revenue growth of the in-situ hybridization market, as a number of research studies have developed viral RNA detection techniques using in-situ hybridization. (emergenresearch.com)
- Demand for procedure is expected to increase following increasing usage of in-situ hybridization for diagnosis of virus and localization of specific location on viral genome, which is expected to drive market revenue growth over the coming years. (emergenresearch.com)
- If Wolf-Hirschhorn is suspected during pregnancy, genetic testing can also be performed as well as a more sophisticated test called fluorescent in situ hybridization (FISH). (verywellhealth.com)
- In situ hybridization confirmed these findings. (bvsalud.org)
- These probes are used in nucleic acid hybridization, in situ hybridization and other molecular biology procedures. (jrank.org)
- Rates of pathologic complete practice due to its high cost and the extensive resources required, response (pCR) following anthracycline/taxane chemotherapy are surrogate definitions of the subtype based on semiquantitative 25%-35%, and patients achieving pCR have better outcomes from IHC scoring of ER, PR, and in situ hybridization tests for HER2 among those patients with TNBC [11]. (2medicalcare.com)
- By use of in situ hybridization to rat tibia, expression of LRP5 was detected in areas of bone involved in remodeling. (elsevier.com)
- Mks1 expression in mouse embryos, as determined by in situ hybridization, agrees well with the tissue phenotype of MKS. (nature.com)
- In situ hybridization analysis of Mks1 expression in mouse embryos. (nature.com)
- The syndrome diagnosis is clinical based on physical and behavioral data which can be confirmed by the analysis of chromosome 15 segment (q11-q13) through methylation or in situ hybridization 10 . (bvsalud.org)
Fluorescent1
- Another method, called comparative genome hybridization, works with fluorescent DNA snippets and shows gaps and duplications in the genome more precisely. (mpg.de)
Genome7
- Whole-exome and whole-genome sequencing may become a first-line clinical test for some naive diagnostic cases, but classic genetic tests will continue to be used for the high analytical sensitivity of specific defects and for the confirmation of genome findings. (nature.com)
- M. S. Mehes, K. K. Nkongolo and P. Michael, "Genetic Analysis of Pinus strobus and Pinus monticola Populations from Canada Using ISSR and RAPD Markers: Development of Genome-Specific SCAR Markers," Plant Systematics and Evolution, Vol. 267, No. 1-4, 2007, pp. 47-63. (scirp.org)
- Also covered are molecular genetic techniques for genome engineering. (cshlpress.com)
- Additional experiments introduce fundamental techniques in yeast genomics, including both performance and interpretation of Synthetic Genetic Array analysis, multiplexed whole genome and barcode sequencing, and comparative genomic hybridization to DNA arrays. (cshlpress.com)
- An appealing alternative to characterize sequence polymorphisms among related organisms is array Comparative Genome Hybridization (array CGH). (biomedcentral.com)
- Bioinformatics uses sophisticated analyses of large amounts of genetic information to clarify the relationships between species, explain the evolution of groups of genes, and assemble information from genome sequencing projects. (cdc.gov)
- No genetic anomalies could be identified by comparative genome hybridisation analysis of their genomes or by analysis of genes known to be associated with these types of anomalies. (cdc.gov)
Role of hybridization3
- The role of hybridization and introgression in the diversification of animals. (cell.com)
- The role of hybridization in lineage divergence and speciation remains a fundamental question of biology. (oxy.edu)
- the role of hybridization, limited gene flow and natural selection in shaping patterns of population structure. (wikidot.com)
Polymerase chain re1
- We used Spectral Karyoryping (SKY), mapping with fluorescently labeled genomic clones (FISH), comparative genomic hybridization (CGH), expression array, real time polymerase chain reaction and Western blot to analyze 15 primary adenocarcinoma and 9 pairs of high and low invasive cell cultures to detect molecular changes. (cdc.gov)
Introgression9
- Rates of hybridization and introgression are increasing dramatically worldwide because of translocations of organisms and habitat modifications by humans. (cell.com)
- Controversy has surrounded the setting of appropriate conservation policies to deal with hybridization and introgression. (cell.com)
- Extinction by hybridization and introgression. (cell.com)
- Hybridization and introgression between introduced and native fish. (cell.com)
- Natural hybridization and introgression in fishes: methods of detection and genetic interpretations. (cell.com)
- Contact zones provide the testing ground to directly measure both the consequences of genetic exchange between divergent lineages and the selective forces that mediate introgression. (oxy.edu)
- Until it is proven that absolute purity is significant, and can be indisputably identified, the NABR includes all Bison for their genetic contribution, but can exclude introgression occurring since 1492 , i.e. hybrids, by process of genetic testing and ethical management. (bisoncentral.com)
- should these birds be included in releases, genetic introgression throughout the tiny remaining population may occur. (speciesonthebrink.org)
- In Uzifly, population diversity results from effective migration rate of the Uzifly and crossing with different geographic populations that likely cause cytoplasmic introgression through hybridization events. (entomoljournal.com)
Interspecific3
- Interspecific hybridization can result in significant shifts in allele frequencies. (scirp.org)
- Extent, Processes and Evolutionary Impact of Interspecific Hybridization in Animals," Philosophical Transactions of the Royal Society of Biological Sciences, Vol. 363, No. 1505, 2008, pp. 2805-2811. (scirp.org)
- The transport of new resistance genes into potato throug the use of interspecific hybridization at diploid level. (gate2biotech.com)
Species20
- The aim of this study was to demonstrate refugia isolation and subsequent secondary contact between two perennial Asclepioid species and to assess the genetic consequences of the secondary contact. (datadryad.org)
- We modeled the range shift of two ecologically distinct Vincetoxicum species using the species distribution model (SDM) and assessed the genetic consequences of secondary contact by combining morphological and genetic approaches. (datadryad.org)
- Gregor 1938 ) in order to conserve the genetic diversity and long-term adaptive potential of species that occur in Scandinavia (Lundqvist et al. (springer.com)
- This confirms the genetic closeness of P. mariana and P. rubens species. (scirp.org)
- I am particularly interested in how speciation in the marine environment occurs, hybridization between species, and population genetics of marine organisms. (rollins.edu)
- While I am continuing to investigate hybridization between two sister species of sea stars in the Northwest Atlantic (Asterias forbesi and A. rubens), I am also collaborating on a population genetics study of the white mangrove in the Caribbean. (rollins.edu)
- Hybridization has contributed to the extinction of many species through direct and indirect means. (cell.com)
- Hybridization of bird species. (cell.com)
- Comparative Genomic Hybridization (CGH) with DNA microarrays has many biological applications including surveys of copy number changes in tumorogenesis, species detection and identification, and functional genomics studies among related organisms. (biomedcentral.com)
- New genetic tests show that all eastern coyotes are actually a mix of three species: coyote, wolf and dog. (iflscience.com)
- In other words, there is no single new genetic entity that should be considered a unique species. (iflscience.com)
- The more recent date for the dog hybridization likely results from a cross-species breeding event at the very leading edge of the wave of colonizing coyotes in the east, possibly after a few females first spanned the St Lawrence seaway into upstate New York, where they would have encountered abundant feral dogs, but no other coyotes. (iflscience.com)
- Hybridization with dogs is most likely along the leading edge of expanding coyote populations, where same-species breeding opportunities are hard to come by. (iflscience.com)
- The percent of the total genetic markers recognized as historic lineage present in one individual of a particular species population. (bisoncentral.com)
- Using this germplasm, physiology was linked to the transcriptome using RNASeq and this was used, alongside extensive high throughput phenotypic data obtained in world-leading platforms at three partner sites, to determine regulatory networks underpinning the genetic architecture of drought tolerance in all three species. (europa.eu)
- In his book Genetics and the Origin of Species , published in 1937, Dobzhansky argued that genetic mutations were sources of variability that, through natural selection, could lead to evolutionary change, and he suggested that these processes could lead to speciation of populations that are isolated long enough. (crev.info)
- Previous work on a variety of marine bivalve species uncovered substantial genetic inviability among the offspring of inbred crosses, suggesting a large load of early-acting deleterious recessive mutations. (datacite.org)
- The seven chromosome scaffolds were anchored to a previously published genetic linkage map with a high degree of synteny and comparisons to genomes of closely related species within the Rosoideae revealed chromosome-scale rearrangements that have occurred over relatively short evolutionary periods. (nibio.no)
- In my opinion, there are much nicer outcomes of hybridizations with species only distantly related. (kingsnake.com)
- Research interests: population ecology, molecular ecology of mammals, animal behavior, metapopulation theory, hybridization and species boundaries in mammals, distribution, taxonomy and population genetics of ground squirrels. (rusmam.ru)
Genomic9
- Genomic technologies are reaching the point of being able to detect genetic variation in patients at high accuracy and reduced cost, offering the promise of fundamentally altering medicine. (nature.com)
- It uses comparative genomic hybridization micro-array technology to identify areas with differences in hybridization between patient and control DNA. (medgadget.com)
- The new device for enhanced prenatal diagnosis, developed by Genetadi "is based on Comparative Genomic Hybridisation" (aCGH) microarray technology. (medgadget.com)
- In this study, genetic variability among isolates was determined by DNA hybridization analysis of restriction endonuclease (RE) genomic digests with cloned hemolysin genes, hemolysin being a putative virulence factor. (usda.gov)
- We report the first comprehensive investigation of DNA copy number across multitude of genes in NF1 tumors using high-resolution array comparative genomic hybridization (CGH), with the aim to identify molecular signatures that delineate malignant from benign NF1 tumors. (aacrjournals.org)
- Rickert CH, Simon R, Bergmann M, Dockhorn-Dworniczak B, Paulus W. Comparative genomic hybridization in pineal germ cell tumors. (medscape.com)
- Isolated populations with novel phenotypes present an exciting opportunity to uncover the genetic basis of ecologically significant adaptation, and genomic scans have often, but not always, led to candidate genes directly related to an adaptive phenotype. (datacite.org)
- Sanger sequencing or DNA-enrichment methods and massively parallel nucleotide sequencing) and quantitative deletion/duplication (e.g., multiple ligation-dependent probe amplification (MLPA), quantitative PCR, or array comparative genomic hybridization) methodologies to identify disease-associated, protein-coding variants in genes associated with this clinical spectrum. (concertgenetics.com)
- Additionally, Prader-Willi and Angelman syndromes are the main recognized human diseases determined by mechanisms of genomic imprinting, that is, a genetic phenomenon in which certain genes are expressed by only one allele 7 . (bvsalud.org)
Speciation2
- Phylogeography, hybridization and speciation. (cell.com)
- Understanding how environmental stress alters the consequences of hybridization is important, because the rate of hybridization and the likelihood of hybrid speciation both appear elevated in harsh, disturbed or marginal habitats. (datacite.org)
Genes3
- Within this new genetic study, scientists now have a catalog of genes for more than 60 kinds of citrus. (discovermagazine.com)
- Genetic testing looks for these changes to individual genes. (healthline.com)
- Over 400 genetic syndromes that include hearing loss have been described, including two of the most common: Pendred syndrome (associated with pathogenic variants in SLC26A4 ) and Usher syndrome (associated with pathogenic variants in multiple genes). (concertgenetics.com)
Mutations4
- The study shows how the fruit emerged at a time of geological upheaval more than 8 million years ago in Southern Asia and spread thanks to genetic mutations that produced more palatable fruit for animals - as well as our human ancestors. (discovermagazine.com)
- Though the genetic changes associated with lung cancer are not well understood, the pattern of mutations observed in lung adenocarcinoma from tobacco exposed patients is distinct from that of lung adenocarcinoma from unexposed patients. (cdc.gov)
- Forward genetic analysis using OCT screening identifies Sfxn3 mutations leading to progressive outer retinal degeneration in mice. (bvsalud.org)
- Genetic engineered heat resistant DNA polymerases, that have proofreading functions and make fewer mutations in the amplified DNA products, are available commercially. (jrank.org)
Chromosome3
- Research and utilization of genetic systems controlling growth and development of wheat by means of chromosome manipulations. (gate2biotech.com)
- Wolf-Hirschhorn syndrome is caused by a missing piece (deletion) of genetic material near the end of the short (p) arm of chromosome 4 . (verywellhealth.com)
- Introduction: Prader-Willi syndrome (PWS) is a neurobehavioral genetic disease whose cause is failure on chromosome 15. (bvsalud.org)
Phenotypic2
- We present an integrated genetic and physical map across the P. vulgaris S locus flanked by phenotypic and DNA sequence markers. (molcyt.org)
- 2014) Geographical and environmental gradients shape phenotypic trait variation and genetic structure in Populus trichocarpa . (wikidot.com)
Probe2
- Genetic testing must be available globally through validated simple technologies for molecular diagnostics (such as direct PCR, linkage analysis or multiplex ligation-dependent probe amplification). (nature.com)
- Plasmids carrying the vanA gene cluster were detected by using S1 nuclease digestion followed by hybridization with a vanA probe ( 4 ). (cdc.gov)
Detection4
- Detection of mRNA molecules and studying functional state of cells are some major applications that have enabled researchers to gain insights on disease prognosis, drug discovery and development, and use of biomarkers for detection of genetic disorders. (emergenresearch.com)
- The detection of prenatal structural anomalies should lead to further genetic evaluation so that many of these conditions can be identified before birth. (medscape.com)
- Constant monitoring of genetic changes in the circulating influenza A(H1N1)pdm09 viruses is important for maintaining the sensitivity of molecular detection assays. (who.int)
- Influenza detection was genetic material of influenza viruses. (who.int)
Hybrids2
- The objective of the present study was to assess the level of genetic variation in populations of P. mariana × P. rubens hybrids derived from artificial crosses. (scirp.org)
- Any policy that deals with hybrids must be flexible and must recognize that nearly every situation involving hybridization is different enough that general rules are not likely to be effective. (cell.com)
Diagnosis3
- Genetic tests are one part of the diagnosis process. (healthline.com)
- Genetic testing is necessary to confirm the diagnosis. (verywellhealth.com)
- In about half of the cases a genetic diagnosis is not possible, leaving the patient with the uncertainty of the origin of the problem", says Stefan Mundlos from the Max Planck Institute for Molecular Genetics and Charité - Universitätsmedizin Berlin. (mpg.de)
Phylogeography1
- Research interests: fauna, taxonomy, hybridization and phylogeography of mammals of the Russian Plain and adjacent territories. (rusmam.ru)
Cytogenetic1
- Cytogenetic testing can detect translocations and other genetic abnormalities. (medlineplus.gov)
Population genetics1
- I have also worked on hybridization and population genetics studies of blue mussels and sea urchins. (rollins.edu)
Diploid4
- Breeders need to understand the genetic make-up of today's domesticated diploid bananas for their crosses between cultivars, and this study is a major first step toward the characterization in great detail of many of these cultivars. (jpost.com)
- Survival of polyploids in nature depends on several factors, including competition from diploid relatives and increased genetic diversity. (upv.es)
- Furthermore, although diploid individuals were grouped in a single widespread genetic cluster, tetraploids were grouped in two highly differentiated clusters and showed significant isolation by distance. (upv.es)
- This genetic pattern in C. seridis may be related to a minimal gene flow with diploid relatives and/or other genetic factors, such as rare polyploidization events, founder effects or an increased selfing rate. (upv.es)
Amplification2
Variation5
- R. Narendrula and K. Nkongolo, "Genetic Variation in Picea mariana × P. rubens Hybrid Populations Assessed with ISSR and RAPD Markers," American Journal of Plant Sciences , Vol. 3 No. 6, 2012, pp. 731-737. (scirp.org)
- R. C. Lewontin and L. C. Birch, "Hybridization as a Source of Variation for Adaptation to New Environments," Evolution, Vol. 20, No. 3, 1966, pp. 315-336. (scirp.org)
- I am interested in understanding and exploring the genetic composition, connectivity, and variation of aquatic invertebrates populations (mainly marine). (rollins.edu)
- Genetic variation is low (Matsui and Hayashi 1992, Matsui et al. (amphibiaweb.org)
- Genetic drift: variation without innovation, aim or purpose. (crev.info)
Virulence1
- The fi lter was then submerged the potential for a broad and well-characterized set of con- in 2× SSC with gentle agitation, air dried, and the DNA UV trol strains relative to virulence factor amplifi cation and (254 nm) cross-linked at 120,000J/cm2 (CL-1000 cross- confi rmed by Southern hybridization. (cdc.gov)
Analysis10
- Results: Multivariate analysis, model-based Bayesian analysis, and non-model-based discriminant analysis of principal components confirmed the hybridization between V. atratum and V. japonicum. (datadryad.org)
- In addition, Bayesian-based clustering analysis and coalescent-based estimates of long-term gene flow showed patterns of introgressive hybridization in three morphologically 'pure' V. japonicum populations. (datadryad.org)
- Complete genetic examination, pedigree analysis, anthropometric measurements and radiological studies were carried out. (who.int)
- We have employed a combination of classical genetics and three-point crosses with molecular genetic analysis of recombinants to generate the map. (molcyt.org)
- F. C. Yeh and T. J. B. Boyle, "Population Genetic Analysis of Codominant and Dominant Markers and Quantitative Traits," Belgian Journal of Botany, Vol. 129, 1997, pp. 157-163. (scirp.org)
- These methods emphasize combinations of classical and modern genetic approaches, including isolation and characterization of mutants, two-hybrid analysis, tetrad analysis, complementation, and recombination. (cshlpress.com)
- Disruption of the target gene was confirmed by Southern hybridization analysis. (mmrrc.org)
- Spoligotype analysis ( 13 ) is a form of DNA sequencing by hybridization. (cdc.gov)
- The M group shows the worst outcomes and the IM genetic analysis of breast cancer is not easily available in clinical group shows the best outcomes [8]. (2medicalcare.com)
- Combining genetic analysis with structural modeling of specific Tudor domains, we propose that these domains serve as `docking platforms' for polar granule assembly. (biologists.com)
Mutation5
- These tests can tell your doctor whether your cancer is related to a genetic mutation and what treatment might work best. (healthline.com)
- The genetic factors are less detailed, although some genetic profiles, such as the E-cadherin, IL-1B, mutation, are known to increase the risk of developing this malignancy (6,10,11). (who.int)
- Genetic data indicate that this mutation resulted in reduced viability of the homozygous mutant mice. (mmrrc.org)
- In most cases, this is not an inherited genetic disorder but rather a mutation that occurs spontaneously. (verywellhealth.com)
- PubMed MEDLINE and Cochrane Library databases, factors are less detailed, although some genetic profiles, retrieving all the articles published from December 1971 such as the E-cadherin, IL-1B , mutation, are known to to March 2022. (who.int)
Pulsed-field gel elect1
- Colonies from each mating experiment were subjected to digestion with Sma I and pulsed-field gel electrophoresis to investigate genetic relatedness ( 1 ). (cdc.gov)
Populations4
- Moreover, our findings show that the pattern of hybridization between V. atratum and V. japonicum is unidirectional and differs among populations. (datadryad.org)
- Table (.csv format) with data on 17 morphological characters studied on hybridization between Vincetoxicum atratum, V. japonicum and hybrid populations in Japan. (datadryad.org)
- Progenies from backcross populations created through a series of controlled pollinations among P. mariana and P. rubens trees across the hybridization index were analyzed. (scirp.org)
- We assessed fitness, morphometrics and molecular genetic composition over 14 generations of hybridization between two highly divergent populations of the marine copepod Tigriopus californicus. (datacite.org)
Phenotype2
- It was previously found that genetic variability among Leptospira borgpetersenii serovar hardjo type hardjobovis (H-HB) isolates correlated with geographic location, and that genetically variable isolates differed in phenotype during experimental infection. (usda.gov)
- PWS is the most common phenotype of genetic obesity. (bvsalud.org)
Heterogeneity3
- Genetic heterogeneity of xeroderma pigmentosum demonstrated by somatic cell hybridization. (semanticscholar.org)
- SRS may comprise different disorders with clinically similar phenotypes or may result from disruption of different components of a single biochemical or endocrinological pathway, in either case reflecting its genetic heterogeneity. (bmj.com)
- Given the genetic heterogeneity of hearing loss, selecting a comprehensive testing approach such as a hearing loss gene panel may be the optimal test once obvious conditions have been excluded. (concertgenetics.com)
Analyses1
- To assess the origins, distinctness and conservation value of ecotypes, it is appropriate to use a combination of molecular-genetic analyses (Lowe et al. (springer.com)
Diversity3
- The polyploid origin of C. seridis, the genetic diversity and population structure of the three cytotypes, and the degree of genetic differentiation among them were analyzed in seven mixed-ploidy zones, involving different subspecies and ecological conditions. (upv.es)
- When compared with the related C. aspera, a low genetic diversity was observed in C. seridis, which is uncommon in tetraploids. (upv.es)
- This section provides a small set of examplesout of the huge number of genetic diversity studies, conducted in both wild andcultivated plants. (daleko.space)
Geographic2
- Hybridization was performed using digests of over 200 different isolates from different geographic regions. (usda.gov)
- For my doctoral research, I researched the geographic distribution and hybridization levels between Mytilus spp. (rollins.edu)
Biotech1
- Genetadi Biotech (Derio, Spain) has developed a chip that is capable of detecting 150 different genetic syndromes from a few milliliters of amniotic fluid. (medgadget.com)
Plant2
- Production of new plant genetic resources for wheat resistance breeding by wide hybridization and with utilization of tissue cultures. (gate2biotech.com)
- However, recent studies have found that natural hybridization has played an important role in the evolution of many plant and animal taxa. (cell.com)
FISH1
- This paper reviews the relevant literature on advantages and limitations of Comet-FISH and MN-FISH assays application in genetic toxicology. (nih.gov)
Fetal1
- Wolf-Hirschhorn syndrome causes malformations in most parts of the body because the genetic error occurs during fetal development. (verywellhealth.com)
20211
- 2021): New phenotyping tools for soybean breeding and genetic resources characterization. (boku.ac.at)
Lineage1
- VR-MRSA belongs to sequence type (ST) 8 and is phylogenetically related to the community-associated (CA) MRSA USA300 genetic lineage that has rapidly disseminated in the United States and the northern region of South America (USA300-Latin American variant [USA300-LV]) ( 1 , 2 ). (cdc.gov)
Differences3
- We concluded that these differences in the genetic consequences of secondary contact are caused by historical colonization processes and/or natural selection. (datadryad.org)
- Differences in PCR product size were consistent with differences observed by aforementioned hybridization. (usda.gov)
- Despite the large differences in the environment and lifestyle of these countries, they still share genetic and cultural similarities and have common scientific societies. (who.int)
Patterns1
- However, genetic forms of hearing loss can follow autosomal, X-linked, or mitochondrial inheritance patterns. (concertgenetics.com)
Array1
- The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. (mmrrc.org)
Anomalies1
- A diagnostic evaluation of craniofacial anomalies, either isolated or as part of a genetic syndrome was conducted on 25 patients [8 females, 17 males], age range 2 months to 47 years. (who.int)
Chromosomes1
- A study by Sokolova et al of 9 genetic markers for detecting urothelial carcinoma showed that polysomy of chromosomes 3, 7, and 17 and deletion of 9p21 were the most sensitive and specific markers, detecting 95% of recurrent urothelial carcinomas. (medscape.com)
Autosomal recessive3
- Autosomal recessive is a pattern of inheritance characteristic of some genetic disorders. (genome.gov)
- Sickle cell anemia is an example of an autosomal recessive genetic disorder. (genome.gov)
- The majority of prelingual deafness is genetic, typically follows an autosomal recessive inheritance pattern, and is non-syndromic. (concertgenetics.com)
Characterization1
- Hoang et al the dominant method in genetics-based diagnostic testing Genetic characterization for influenza in Viet Nam because it is less expensive. (who.int)
Disorders4
- Clinical molecular genetic testing is transforming personalized medicine and is appropriate for a range of applications, such as rare disease diagnostics and predictive testing for common disorders. (nature.com)
- Autosomal dominant is a pattern of inheritance characteristic of some genetic disorders. (genome.gov)
- Many genetic disorders can be detected early in pregnancy using various noninvasive and invasive techniques. (medscape.com)
- A team of researchers at the Max Planck Institute for Molecular Genetics and Charité - Universitätsmedizin Berlin led by human geneticists Malte Spielmann and Stefan Mundlos analyzed clinical samples from patients with genetic developmental disorders with the Hi-C method. (mpg.de)
Outcomes1
- Genetic cancer risk assessment in general practice: systematic review of tools available, clinician attitudes, and patient outcomes. (cdc.gov)