High molecular weight mucoproteins that protect the surface of EPITHELIAL CELLS by providing a barrier to particulate matter and microorganisms. Membrane-anchored mucins may have additional roles concerned with protein interactions at the cell surface.
A gel-forming mucin that is predominantly expressed by submucosal glands of airway tissues and the SUBLINGUAL GLAND. It is one of the principal components of high molecular weight salivary mucin.
Mucins that are found on the surface of the gastric epithelium. They play a role in protecting the epithelial layer from mechanical and chemical damage.
A transmembrane mucin that is found in a broad variety of epithelial tissue. Mucin-4 may play a role in regulating cellular adhesion and in cell surface signaling from the ERBB-2 RECEPTOR PROTEIN-TYROSINE KINASE. Mucin-4 is a heterodimer of alpha and beta chains. The alpha and beta chains result from the proteolytic cleavage of a precursor protein.
Carbohydrate antigen elevated in patients with tumors of the breast, ovary, lung, and prostate as well as other disorders. The mucin is expressed normally by most glandular epithelia but shows particularly increased expression in the breast at lactation and in malignancy. It is thus an established serum marker for breast cancer.
A gel-forming mucin that is primarily found on the surface of gastric epithelium and in the RESPIRATORY TRACT. Mucin 5AC was originally identified as two distinct proteins, however a single gene encodes the protein which gives rise to the mucin 5A and mucin 5C variants.
A gel-forming mucin that is predominantly associated with the gastric epithelium.
A gel-forming mucin found predominantly in SMALL INTESTINE and variety of mucous membrane-containing organs. It provides a protective, lubricating barrier against particles and infectious agents.
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.
The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.
A membrane-bound mucin subtype that is primarily found in INTESTINAL MUCOSA. Two closely-related subtypes of this protein have been identified in humans.
A glandular epithelial cell or a unicellular gland. Goblet cells secrete MUCUS. They are scattered in the epithelial linings of many organs, especially the SMALL INTESTINE and the RESPIRATORY TRACT.
The viscous secretion of mucous membranes. It contains mucin, white blood cells, water, inorganic salts, and exfoliated cells.
A subcategory of mucins that contain SIALIC ACID.
Immunized T-lymphocytes which can directly destroy appropriate target cells. These cytotoxic lymphocytes may be generated in vitro in mixed lymphocyte cultures (MLC), in vivo during a graft-versus-host (GVH) reaction, or after immunization with an allograft, tumor cell or virally transformed or chemically modified target cell. The lytic phenomenon is sometimes referred to as cell-mediated lympholysis (CML). These CD8-positive cells are distinct from NATURAL KILLER CELLS and NATURAL KILLER T-CELLS. There are two effector phenotypes: TC1 and TC2.
The phenomenon of target cell destruction by immunologically active effector cells. It may be brought about directly by sensitized T-lymphocytes or by lymphoid or myeloid "killer" cells, or it may be mediated by cytotoxic antibody, cytotoxic factor released by lymphoid cells, or complement.
Tumors or cancer of the PANCREAS. Depending on the types of ISLET CELLS present in the tumors, various hormones can be secreted: GLUCAGON from PANCREATIC ALPHA CELLS; INSULIN from PANCREATIC BETA CELLS; and SOMATOSTATIN from the SOMATOSTATIN-SECRETING CELLS. Most are malignant except the insulin-producing tumors (INSULINOMA).
The genetic region which contains the loci of genes which determine the structure of the serologically defined (SD) and lymphocyte-defined (LD) TRANSPLANTATION ANTIGENS, genes which control the structure of the IMMUNE RESPONSE-ASSOCIATED ANTIGENS, HUMAN; the IMMUNE RESPONSE GENES which control the ability of an animal to respond immunologically to antigenic stimuli, and genes which determine the structure and/or level of the first four components of complement.
A cell line derived from cultured tumor cells.
They are oval or bean shaped bodies (1 - 30 mm in diameter) located along the lymphatic system.
A storage reservoir for BILE secretion. Gallbladder allows the delivery of bile acids at a high concentration and in a controlled manner, via the CYSTIC DUCT to the DUODENUM, for degradation of dietary lipid.
Tumors or cancer of the gallbladder.
A genus of chiefly Eurasian and African land snails including the principal edible snails as well as several pests of cultivated plants.
Presence or formation of GALLSTONES in the BILIARY TRACT, usually in the gallbladder (CHOLECYSTOLITHIASIS) or the common bile duct (CHOLEDOCHOLITHIASIS).
An emulsifying agent produced in the LIVER and secreted into the DUODENUM. Its composition includes BILE ACIDS AND SALTS; CHOLESTEROL; and ELECTROLYTES. It aids DIGESTION of fats in the duodenum.
Proteins that share the common characteristic of binding to carbohydrates. Some ANTIBODIES and carbohydrate-metabolizing proteins (ENZYMES) also bind to carbohydrates, however they are not considered lectins. PLANT LECTINS are carbohydrate-binding proteins that have been primarily identified by their hemagglutinating activity (HEMAGGLUTININS). However, a variety of lectins occur in animal species where they serve diverse array of functions through specific carbohydrate recognition.
The space and structures directly internal to the TYMPANIC MEMBRANE and external to the inner ear (LABYRINTH). Its major components include the AUDITORY OSSICLES and the EUSTACHIAN TUBE that connects the cavity of middle ear (tympanic cavity) to the upper part of the throat.
Inflammation of the middle ear with a clear pale yellow-colored transudate.
Inflammation of the MIDDLE EAR including the AUDITORY OSSICLES and the EUSTACHIAN TUBE.
Endoscopes for examining the interior of the larynx.
A mass of KERATIN-producing squamous EPITHELIUM that resembles an inverted (suck-in) bag of skin in the MIDDLE EAR. It arises from the eardrum (TYMPANIC MEMBRANE) and grows into the MIDDLE EAR causing erosion of EAR OSSICLES and MASTOID that contains the INNER EAR.
A phylum of gram-negative bacteria containing seven class-level groups from a wide variety of environments. Most members are chemoheterotrophs.
One of the three domains of life (the others being Eukarya and ARCHAEA), also called Eubacteria. They are unicellular prokaryotic microorganisms which generally possess rigid cell walls, multiply by cell division, and exhibit three principal forms: round or coccal, rodlike or bacillary, and spiral or spirochetal. Bacteria can be classified by their response to OXYGEN: aerobic, anaerobic, or facultatively anaerobic; by the mode by which they obtain their energy: chemotrophy (via chemical reaction) or PHOTOTROPHY (via light reaction); for chemotrophs by their source of chemical energy: CHEMOLITHOTROPHY (from inorganic compounds) or chemoorganotrophy (from organic compounds); and by their source for CARBON; NITROGEN; etc.; HETEROTROPHY (from organic sources) or AUTOTROPHY (from CARBON DIOXIDE). They can also be classified by whether or not they stain (based on the structure of their CELL WALLS) with CRYSTAL VIOLET dye: gram-negative or gram-positive.
Constituent of 30S subunit prokaryotic ribosomes containing 1600 nucleotides and 21 proteins. 16S rRNA is involved in initiation of polypeptide synthesis.
The section of the alimentary canal from the STOMACH to the ANAL CANAL. It includes the LARGE INTESTINE and SMALL INTESTINE.
Non-digestible food ingredients mostly of a carbohydrate base that improve human health by selectively stimulating the growth and/or activity of existing BACTERIA in the COLON.
A subspecialty of internal medicine concerned with the study of the physiology and diseases of the digestive system and related structures (esophagus, liver, gallbladder, and pancreas).
Sites on an antigen that interact with specific antibodies.
Tumors or cancer of the COLON.
The largest class of organic compounds, including STARCH; GLYCOGEN; CELLULOSE; POLYSACCHARIDES; and simple MONOSACCHARIDES. Carbohydrates are composed of carbon, hydrogen, and oxygen in a ratio of Cn(H2O)n.
Human colonic ADENOCARCINOMA cells that are able to express differentiation features characteristic of mature intestinal cells such as the GOBLET CELLS.
A trisaccharide antigen expressed on glycolipids and many cell-surface glycoproteins. In the blood the antigen is found on the surface of NEUTROPHILS; EOSINOPHILS; and MONOCYTES. In addition, CD15 antigen is a stage-specific embryonic antigen.
A cell surface receptor involved in regulation of cell growth and differentiation. It is specific for EPIDERMAL GROWTH FACTOR and EGF-related peptides including TRANSFORMING GROWTH FACTOR ALPHA; AMPHIREGULIN; and HEPARIN-BINDING EGF-LIKE GROWTH FACTOR. The binding of ligand to the receptor causes activation of its intrinsic tyrosine kinase activity and rapid internalization of the receptor-ligand complex into the cell.
Granular leukocytes with a nucleus that usually has two lobes connected by a slender thread of chromatin, and cytoplasm containing coarse, round granules that are uniform in size and stainable by eosin.
A family of synthetic protein tyrosine kinase inhibitors. They selectively inhibit receptor autophosphorylation and are used to study receptor function.
A salivary gland on each side of the mouth below the TONGUE.
A 6-kDa polypeptide growth factor initially discovered in mouse submaxillary glands. Human epidermal growth factor was originally isolated from urine based on its ability to inhibit gastric secretion and called urogastrone. Epidermal growth factor exerts a wide variety of biological effects including the promotion of proliferation and differentiation of mesenchymal and EPITHELIAL CELLS. It is synthesized as a transmembrane protein which can be cleaved to release a soluble active form.
Works containing information articles on subjects in every field of knowledge, usually arranged in alphabetical order, or a similar work limited to a special field or subject. (From The ALA Glossary of Library and Information Science, 1983)
A biomineral consisting of layers of crystalized ARAGONITE, separated by interlamellar layers of organic matrix containing beta-CHITIN, and surrounded by an intercrystalline organic membrane of silk fibroin. Nacre is the iridescent substance better known as mother-of-pearl that is secreted by MOLLUSCS.
The application of LUBRICANTS to diminish FRICTION between two surfaces.
A plant genus of the family Orobanchaceae that is parasitic on the roots of other plants. Members contain the flavones, APIGENIN and LUTEOLIN.
A genus of pearl oysters in the family Pteriidae, class BIVALVIA. Both cultured and natural pearls are obtained from species in the genus. They are distinct from the distantly related, edible true oysters of the family OSTREIDAE.
... also known as mucin 16 or MUC16 is a protein that in humans is encoded by the MUC16 gene. MUC16 is a member of the mucin family ... "Distinct evolution of the human carcinoma-associated transmembrane mucins, MUC1, MUC4 AND MUC16". Gene. 373: 28-34. doi:10.1016 ... Perez BH, Gipson IK (Nov 2008). "Focus on Molecules: human mucin MUC16". Experimental Eye Research. 87 (5): 400-1. doi:10.1016/ ... Mucin 16 is a membrane associated mucin that possesses a single transmembrane domain. A unique property of MUC16 is its large ...
"Immunohistochemical study of mucins in human intestinal spirochetosis". Human Pathology. 62: 126-133. doi:10.1016/j.humpath. ... Naresh, Ram; Hampson, David J. (1 January 2010). "Attraction of Brachyspira pilosicoli to mucin". Microbiology (Reading, ... Brachyspira species include pathogens in pigs, birds, dogs, and humans. B. pilosicoli colonizes millions of humans worldwide, ... Westerman, L. J. (2013). Human Intestinal Spirochaetosis (PhD thesis).[permanent dead link] Le Roy, Caroline I.; Mappley, Luke ...
2001). "Synthesis and localization of the mucin-associated TFF-peptides in the human uterus". Cell Tissue Res. 303 (1): 109-15 ... By cross-linking mucins through the bivalent binding of this glycotope, the trefoil factors are then able to reversible ... 1997). "Human intestinal trefoil factor is expressed in human hypothalamus and pituitary: evidence for a novel neuropeptide". ... 1997). "The three human trefoil genes TFF1, TFF2, and TFF3 are located within a region of 55 kb on chromosome 21q22.3". ...
Patton, Stuart (2001). "MUC1 and MUC-X, Epithelial Mucins of Breast and Milk". Bioactive Components of Human Milk. Advances in ... "Polymorphic forms of the epithelial mucin, PAS-I (MUC1), in milk of Holstein cows (Bos taurus)". Comparative Biochemistry and ... Patton began extensive work analyzing the nature of human milk, with special emphasis on its mucins. He discovered that the ... mucins MUC1 and MUC-X, which are transferred to the milk fat globule upon secretion, have greater size in human milk and ...
... is a mucin encoded by the MUC1 gene in humans. MUC1 is a glycoprotein with extensive O-linked glycosylation of its ... Mucins line the apical surface of epithelial cells in the lungs, stomach, intestines, eyes and several other organs. Mucins ... Mucin 1, cell surface associated (MUC1), also called polymorphic epithelial mucin (PEM) or epithelial membrane antigen or EMA, ... "Molecular cloning and expression of human tumor-associated polymorphic epithelial mucin". The Journal of Biological Chemistry. ...
Mucins are stored in granules inside the goblet cells before being released to the lumen of the organ. Mucin secretion in the ... List of human cell types derived from the germ layers Hodges, R.R.; Dartt, D.A. (2010). "Conjunctival Goblet Cells". ... In the conjunctiva goblet cells are a source of mucin in tears and they also secrete different types of mucins onto the ocular ... Goblet cells are simple columnar goblet shaped like epithelial cells that secrete gel-forming mucins, like mucin MUC5AC. The ...
Miraculin, is a glycoprotein extracted from Synsepalum dulcificum a berry which alters human tongue receptors to recognize sour ... One example of glycoproteins found in the body is mucins, which are secreted in the mucus of the respiratory and digestive ... The viral spike of the human immunodeficiency virus is heavily glycosylated.[8] Approximately half the mass of the spike is ... The sugars when attached to mucins give them considerable water-holding capacity and also make them resistant to proteolysis by ...
"The human proteome in salivary gland - The Human Protein Atlas". www.proteinatlas.org. Retrieved 2017-09-22.. ... Mucins are the principal organic constituents of mucus, the slimy visco-elastic material that coats all mucosal surfaces.[20] ... Examples of other specifically expressed proteins include the digestive amylase enzyme AMY1A, the mucin MUC7 and statherin, all ... In humans, between 0.5 and 1.5 litres of saliva are produced every day.[3] The secretion of saliva (salivation) is mediated by ...
Function in Humans[edit]. Mucins have been found to have important functions in defense against bacterial and fungal infections ... At least 20 human mucin genes have been distinguished by cDNA cloning - MUC1, MUC2, MUC3A, MUC3B, MUC4, MUC5AC, MUC5B, MUC6, ... Li Y, Martin LD, Spizz G, Adler KB (November 2001). "MARCKS protein is a key molecule regulating mucin secretion by human ... Mucin genes encode mucin monomers that are synthesized as rod-shape apomucin cores that are post-translationally modified by ...
In addition to GAGs, mucin is of interest in P-selectin mediated tumor metastasis.[25] Selective removal of mucin results in ... In human, P-selectin has nine repeats while E-selectin contains six and L-selectin has only two. P-selectin is anchored in ... "Heparin and cancer revisited: mechanistic connections involving platelets, P-selectin, carcinoma mucins, and tumor metastasis" ... P-selectin is located on chromosome 1q21-q24, spans , 50 kb and contains 17 exons in humans.[7] P-selectin is constitutively ...
The main component of mucus is a glycoprotein called mucin and the type secreted varies according to the region involved. Mucin ... "Salivary Mucins Protect Surfaces from Colonization by Cariogenic Bacteria". Applied and Environmental Microbiology. 81 (1): ... Saladin, Kenneth (2011). Human Anatomy. McGraw Hill. p. 659. ISBN 9780071222075. .. *^ a b c consultants Daniel Albert et al. ( ... Saladin, Kenneth (2011). Human Anatomy. McGraw Hill. p. 672. ISBN 9780071222075. .. *^ Wood, Jackie D. (2009), " ...
... type mucin simulates the acid mucin true goblet cells). Assessment of relationship to submucosal glands and transitional-type ... Use of the histochemical stain Alcian blue pH 2.5 is also frequently used to distinguish true intestinal-type mucins from their ... "Bile acids induce ectopic expression of intestinal guanylyl cyclase C through nuclear factor-kappaB and Cdx2 in human ...
... and mucins may aid in diagnosis.[45] Most tumors (,90%) are adenocarcinomas.[46] ... a cancer that forms glands or secretes mucin).[3] ... "Possible stem cell origin of human cholangiocarcinoma". World ...
... (MUC 4) is a mucin protein that in humans is encoded by the MUC4 gene. Like other mucins, MUC-4 is a high-molecular ... As a mucin, MUC-4 also alters adhesive properties of the cell. When overexpressed, the disorganization of mucins may reduce ... 1998). "Human mucin gene MUC4: organization of its 5'-region and polymorphism of its central tandem repeat array". Biochem. J. ... MUC-4 belongs to the human mucin family that is membrane-anchored and can range in molecular weight from 550 to 930 kDa for the ...
The mucin-selective protease StcE enables molecular and functional analysis of human cancer-associated mucins. Stacy A. Malaker ... The mucin-selective protease StcE enables molecular and functional analysis of human cancer-associated mucins ... The mucin-selective protease StcE enables molecular and functional analysis of human cancer-associated mucins ... The mucin-selective protease StcE enables molecular and functional analysis of human cancer-associated mucins ...
UDP-glucose and adenyl purines were assessed as described in and mucins were measured by an enzyme-linked lectin assay as ... Coordinated release of nucleotides and mucin from human airway epithelial Calu-3 cells.. Kreda SM1, Okada SF, van Heusden CA, ... Effective mucin dispersion into ASL requires salt and water secretion onto the mucosal surface, but how mucin secretion rate is ... Coordinated release of nucleotides and mucin from human airway epithelial Calu-3 cells ...
Mucin-type O-glycans in human colon and breast cancer: glycodynamics and functions.. Brockhausen I1. ... Mucin-type O-glycans in human colon and breast cancer: glycodynamics and functions ... Mucin-type O-glycans in human colon and breast cancer: glycodynamics and functions ... Mucin-type O-glycans in human colon and breast cancer: glycodynamics and functions ...
In addition to pancreatic and breast tumors, various mucin molecules are known to be produced by other tumors of epithelial ... Specific, major histocompatibility complex-unrestricted recognition of tumor-associated mucins by human cytotoxic T cells. D L ... Specific, major histocompatibility complex-unrestricted recognition of tumor-associated mucins by human cytotoxic T cells ... Specific, major histocompatibility complex-unrestricted recognition of tumor-associated mucins by human cytotoxic T cells ...
The main components of the mucus layer are heavily glycosylated mucins, to which H. pylori can adhere. Mucin glycosylation ... depending on the type of mucins and the ability of the mucins to bind to H. pylori. Tumor-derived mucins and mucins from the ... Here we have examined the H. pylori response to purified mucins from a range of tumor and normal human gastric tissue samples. ... albeit to a lesser degree than human mucins. Expression of genes important for the pathogenicity of H. pylori (babA, sabA, cagA ...
Mucins are highly O-glycosylated glycoproteins ubiquitous in mucous secretions on cell surfaces and in body fluids. Mucin O- ... Mucin type O-glycan biosynthesis - Homo sapiens (human) [ Pathway menu , Organism menu , Pathway entry , Download KGML , Show ... Important modifications of mucin O-glycans include O-acetylation of sialic acid and O-sulfation of galactose and N- ...
... and expression of A antigen could be demonstrated on the mucin of these patients. The resulting two reacting mucins were from ... Heterogeneity of Human Gallbladder Mucin in Bile. Michel J. A. van Wijland, J. Henriëtte Klinkspoor, Laurens Th. de Wit, Ronald ... Heterogeneity of Human Gallbladder Mucin in Bile. Michel J. A. van Wijland, J. Henriëtte Klinkspoor, Laurens Th. de Wit, Ronald ... 1. Human gallbladder mucin has been implicated to play a role in gallstone disease. In spite of this fact relatively little is ...
Santosh Kumar Behera, Ardhendu Bhusan Praharaj, Budheswar Dehury, Sapna Negi, Exploring the role and diversity of mucins in ... Identification of MUC5B Mucin Gene in Human Middle Ear With Chronic Otitis Media†. Authors. *. Hirokazu Kawano MD,. * ... Effects of cigarette smoking on mucin production in human middle ear epithelial cells, International Journal of Pediatric ... Y.P. Chen, H.H. Tong, M.A. James, T, Detection of Mucin Gene Expression in Normal Rat Middle Ear Mucosa by Reverse ...
... was isolated by dilution to extinction of faeces in anaerobic medium containing gastric mucin as the sole carbon and nitrogen s ... The diversity of mucin-degrading bacteria in the human intestine was investigated by combining culture and 16S rRNA-dependent ... Gastric Mucins / metabolism* * Genes, rRNA / genetics * Glucose / metabolism * Gram-Negative Anaerobic Bacteria / ... nov., a human intestinal mucin-degrading bacterium Int J Syst Evol Microbiol. 2004 Sep;54(Pt 5):1469-1476. doi: 10.1099/ijs. ...
Sialylation and sulfation of the carbohydrate chains in respiratory mucins from a patient with cystic fibrosis LO-GUIDICE J.-M ... Expression of Mucin-associated Sulfo-Le^a Carbohydrate Epitopes on Human Colon Carcinoma Cells * * TSUIJI Hitomi ... Inhibition of mucin glycosylation by aryl-N-acetyl-a-galactosaminidases in human colon cancer cells. KUAN S. F. ... Sulfated Lewis^x determinants-a major structural motif in glycans from LS174T HM7 human colon carcinoma mucin CAPON C. ...
NCI-H292 cells, a human pulmonary mucoepidermoid carcinoma cell line capable of expressing mucins (23), were grown in RPMI 1640 ... Thus, the present study shows for the first time that activated human eosinophils cause mucin production in human airway ... Therefore, we studied the effect of human eosinophil activation on mucin MUC5AC production in human airway epithelial (NCI-H292 ... Mucin hypersecretion involves two processes: mucin production and mucin secretion. The present studies show that secreted ...
Identification of MUC5B, MUC5AC and small amounts of MUC2 mucins in cystic fibrosis airway secretions Biochem J (November,1999) ... Cleavage in the GDPH sequence of the C-terminal cysteine-rich part of the human MUC5AC mucin Martin E. Lidell ; Martin E. ... This sequence is also found in the human MUC2 mucin, previously shown to be cleaved at the same site by a slow, non-enzymatic ... We expressed the C-terminal cysteine-rich part of the human MUC5AC mucin in CHO-K1 cells (Chinese-hamster ovary K1 cells) where ...
1995) Binding of Haemophilus influenzae to purified mucins from the human respiratory tract. Infect. Immun. 63:2485-2492. ... i) Immobilized mucin assay.A modified solid-phase mucin binding assay was performed with bovine mucin as described previously ( ... Bacteria were used to inoculate mucin-coated wells (1 μg of mucin/well inoculated with 5 × 106 CFU/well). (B) Mucin-coated ... Group A Streptococci Bind to Mucin and Human Pharyngeal Cells through Sialic Acid-Containing Receptors. Patricia A. Ryan, ...
Recently, we described dysplastic foci depleted of mucins (MDF) in the colon of rats treated with colon-specific carcinogens. ... Identification of Mucin Depleted Foci in the Human Colon. Angelo Pietro Femia, Augusto Giannini, Marilena Fazi, Elena Tarquini ... Identification of Mucin Depleted Foci in the Human Colon. Angelo Pietro Femia, Augusto Giannini, Marilena Fazi, Elena Tarquini ... Identification of Mucin Depleted Foci in the Human Colon. Angelo Pietro Femia, Augusto Giannini, Marilena Fazi, Elena Tarquini ...
The yield was 6 to 7 μg of pure mucin/T75 flask. For comparison with the concentration of mucins isolated from the normal human ... Isolation of normal human gut mucins.The mucus layer was scraped from a resected piece of a normal human colon, placed in cold ... La1-bound mucins were revealed with mouse anti-mucin polyclonal antibodies. Mucin binding was expressed as the absorbance at ... The same bacteria were incubated with normal human gut mucins at pH 5.0 and fixed with paraformaldehyde. La1-bound mucins were ...
These human mucin probes may be important in assessing the abnormal mucins associated with inflammatory diseases or carcinoma ... Degenerate 87-base-pair tandem repeats create hydrophilic/hydrophobic alternating domains in human mucin peptides mapped to ... Degenerate 87-base-pair tandem repeats create hydrophilic/hydrophobic alternating domains in human mucin peptides mapped to ... Degenerate 87-base-pair tandem repeats create hydrophilic/hydrophobic alternating domains in human mucin peptides mapped to ...
Abstract: : Purpose: 15(S)-HETE stimulates release of ocular surface mucins by a mechanism dependent on external Ca2+. This ... Involvement of TRPC Cation Channels in 15(S)-HETE- Regulated Mucin Secretion by Human Corneal Epithelial Cells. ... Involvement of TRPC Cation Channels in 15(S)-HETE- Regulated Mucin Secretion by Human Corneal Epithelial Cells. ... HETE-induced MUC1 mucin secretion by human corneal epithelial cells. Methods: MUC1 secretion was measured by quantitative ...
... but their effects on mucin gene expression in human esophageal adenocarcinoma cells is unknown. Human esophageal adenocarcinoma ... Deoxycholic acid induced MUC2 overexpression in human esophageal adenocarcinoma cells by activation of Nuclear factor-κB ... Bile acids have been linked to esophageal adenocarcinoma and mucin secretion, ... and alterations in MUC2 mucin have been associated with tumor progression in the esophagus. ...
PURPOSE: Mucins are important structural and functional components of the precorneal tear film, yet little is known of their ... MUC5AC mucin is a component of the human precorneal tear film. You will receive an email whenever this article is corrected, ... M M Jumblatt, R W McKenzie, J E Jumblatt; MUC5AC mucin is a component of the human precorneal tear film.. Invest. Ophthalmol. ... CONCLUSIONS: MUC5AC is synthesized by goblet cells of the normal human conjunctiva, and this mucin is a component of ...
The amino termini of the MUC3A and MUC3B mucins are 91% conserved at the amino acid level. Thus, MUC3A and MUC3B have highly ... Human intestinal mucin genes MUC3A and MUC3B are members of a membrane mucin gene family residing at chromosome 7q22. In this ... Initiation of transcription of the MUC3A human intestinal mucin from a TATA-less promoter and comparison with the MUC3B amino ... Initiation of transcription of the MUC3A human intestinal mucin from a TATA-less promoter and comparison with the MUC3B amino ...
Function in Humans[edit]. Mucins have been found to have important functions in defense against bacterial and fungal infections ... At least 20 human mucin genes have been distinguished by cDNA cloning - MUC1, MUC2, MUC3A, MUC3B, MUC4, MUC5AC, MUC5B, MUC6, ... Li Y, Martin LD, Spizz G, Adler KB (November 2001). "MARCKS protein is a key molecule regulating mucin secretion by human ... Mucin genes encode mucin monomers that are synthesized as rod-shape apomucin cores that are post-translationally modified by ...
... and 8 in human fetal tissues to establish the localization and age of onset of expression of each mucin gene during human ... We examined the expression of mucin genes, MUC3, 4, 5AC, 5B, 6, 7, ... Mucin glycoproteins play a key role in the normal function of the airway epithelium. ... Mucins, Pregnancy, Respiratory System ... Developmental expression of mucin genes in the human ...
Zalewska A, Zwierz K, Zółkowski K, Gindzieński A (2002). "Structure and biosynthesis of human salivary mucins". Acta Biochimica ... Mucin-7 is a protein that in humans is encoded by the MUC7 gene. In animals, the MUC7 gene is found in most placental mammals, ... Ge J, Catt DM, Gregory RL (November 2004). "Streptococcus mutans surface alpha-enolase binds salivary mucin MG2 and human ... "Human low-molecular-weight salivary mucin expresses the sialyl lewisx determinant and has L-selectin ligand activity". ...
Mucin-17 is a protein that in humans is encoded by the MUC17 gene. Membrane mucins, such as MUC17, function in epithelial cells ... 2006). "Characterization of human mucin MUC17. Complete coding sequence and organization". J. Biol. Chem. 281 (33): 23676-23685 ... "Entrez Gene: MUC17 mucin 17, cell surface associated". Kitamoto S, Yamada N, Yokoyama S, et al. (2011). "DNA methylation and ... Gum JR Jr; Crawley SC; Hicks JW; Szymkowski DE; Kim YS (Feb 2002). "MUC17, a novel membrane-tethered mucin". Biochem Biophys ...
Mucins are a primary ingredient of the mucus that makes up banana slug slime. Mucin proteins form chains and have sugar ... Mucin patterns protect humans too. Our lungs contain mucus that catches rogue dust particles and pathogens such as viruses and ... Mucin patterns help slugs interact with rough surfaces. Mucins reorganize to coat sand or rocks, allowing slugs to cruise ... The mucins in banana slug slime have to form small networks to coat the tiny spikes of sand. The giraffe saliva mucins, however ...
... copes with the mucin defense barrier. We report that upon in vitro infection of mucin-producing polarized human intestinal ... We identify MUC5AC, a gel-forming mucin, as a component of this structure. Formation of this gel does not depend on ... This phenomenon, which is triggered only by virulent strains, results in accumulation of mucins at the cell apical surface, ... In addition, transcriptional and biochemical analyses of infected cells reveal modulations of mucin gene expression and ...
Mucin genes are very polymorphic, and mucins play a key role in the pathogenesis of CF lung disease; therefore, mucin genes are ... These data provide detailed respiratory mucin gene VNTR allele distributions in CF patients. Our data also show a novel link ... Alleles, Cystic Fibrosis, Genotype, Humans, Lung, Minisatellite Repeats, Mucin 5AC, Phenotype, Polymorphism, Single Nucleotide ... Mucin genes are very polymorphic, and mucins play a key role in the pathogenesis of CF lung disease; therefore, mucin genes are ...
Additionally, these crosslinked polymers did not induce symptoms of toxicity or irritation in either human vaginal explants or ... Inhibition of the transport of HIV in vitro using a pH-responsive synthetic mucin-like polymer system. Biomaterials. 2011 Nov; ... Dive into the research topics of Inhibition of the transport of HIV in vitro using a pH-responsive synthetic mucin-like ... Inhibition of the transport of HIV in vitro using a pH-responsive synthetic mucin-like polymer system. / Mahalingam, Alamelu; ...
... mucins as design equivalents for human mucins to study their conversation with purified catechins and tea extracts. The mucin ... This system of mucin extraction unavoidably excludes the extraction of a fraction of mucins present in the "insoluble" thicker ... Polyphenol treated mucin solutions ended up fitted with Eq. (1) from which J, the typical thickness of the period divided ... In the two forms of mucin, the scattering profile of the inventory solution is described in its entirety with a I(q),q21.7 ...
The mucin-selective protease StcE enables molecular and functional analysis of human cancer-associated mucins. ... test kits for ph The mucin-selective protease StcE enables molecular and functional analysis of human cancer-associated mucins. ... CA125 encodes a protein that is a member of the mucin family. Mucins are high molecular weight, O-glycosylated proteins that ... CA125 encodes a protein that is a member of the mucin family. Mucins are high molecular weight, O-glycosylated proteins that ...
This summary is for the human ortholog.] This gene encodes a member of the mucin protein family. Mucins are high molecular ...
The model is supplemented with human gut microbiota and mucin-covered microcosms. My colleagues and I have applied for funding ... Moreover, these mucins serve as food for commensal bacteria and are highly resistant to protease activity unless destabilised. ... One of the current mantras in microbiology is that bacterial cells in the human body outnumber human cells 10 to one. This ... Moving on to paper of the month, I would just briefly highlight a study by Wu, Mirza and Tan, who used Caco-2 human colonic ...
Eating by humans for millennia devastated Japan s first glow-in-the-dark ice cream using jellyfish to biodegradable. Mucin to ... Mucins are used in artificial tears, food additives, and cosmetic products.[6]. When the cell was exposed to ultraviolet light ... Jellyfish benefits . Mucin keeps our eyeballs moist and is the basis of mucus in the nose that protects against invading ... The gravity issues faced by space-born jellyfish indicate that space-born humans would suffer the same problem if they returned ...
"Highly glycosylated regions of mucins, or glycopeptides, were obtained by proteolysis of human bronchial mucins. They were ... Human airway mucin glycosylation: A combinatory of carbohydrate determinants which vary in cystic fibrosis ... Human respiratory mucins by Lamblin, G Aubert, JP Perini, JM Klein, A Porchet, N Degand, P Roussel, P Published in The European ... "Human respiratory mucins are secreted by goblet cells and mucous glands of the respiratory mucosa. They consist of a broad ...
Distinct Transcriptional Profiles in Engineered Human Ectocervical Tissue Dependent on Menstrual Cycle Phase. (Accepted at ... In summary, I developed three microphysiologic human ectocervical tissue models that differentiate, produce mucins, and respond ... and mucin production, which remained viable for 28 days in vitro. The localization of differentiation-dependent mucins in ... and mucin production, which are important components of barrier defense. Additionally, mRNA transcripts for several mucins not ...
Impact of Mucin on Drug Diffusion: Development of a Straightforward in Vitro Method for the Determination of Drug Diffusivity ... Co-existing colloidal phases of human duodenal aspirates: Intraindividual fluctuations and interindividual variability in ... in the Presence of Mucin. Falavigna, M., Stein, P. C., Flaten, G. E. & di Cagno, M. P., 2020, I: Pharmaceutics. 12, 2, 13 s., ...
The mucin-selective protease StcE enables molecular and functional analysis of human cancer-associated mucins. ... test kits for ph The mucin-selective protease StcE enables molecular and functional analysis of human cancer-associated mucins. ... Human EG-VEGF(Endocrine Gland Derived Vascular Endothelial Growth Factor) ELISA Kit. Human EG-VEGF(Endocrine Gland Derived ... Description: Recombinant Human Endocrine Gland Derived Vascular Endothelial Growth Factor expressed in: Available from E.coli, ...
The mucin-selective protease StcE enables molecular and functional analysis of human cancer-associated mucins. ... test kits for ph The mucin-selective protease StcE enables molecular and functional analysis of human cancer-associated mucins. ... Human LIF(Leukemia Inhibitory Factor) ELISA Kit. Human LIF(Leukemia Inhibitory Factor) ELISA Kit ... The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human LIF. Standards or samples are ...
The mucin-selective protease StcE enables molecular and functional analysis of human cancer-associated mucins. ... test kits for ph The mucin-selective protease StcE enables molecular and functional analysis of human cancer-associated mucins. ... Should the Human BH3 Interacting Domain Death Agonist (Bid) ELISA Kit is proven to show malperformance, you will receive a ... Should the Human BH3 Interacting Domain Death Agonist (Bid) ELISA Kit is proven to show malperformance, you will receive a ...
The mucin-selective protease StcE enables molecular and functional analysis of human cancer-associated mucins. ... test kits for ph The mucin-selective protease StcE enables molecular and functional analysis of human cancer-associated mucins. ... Human KLK10(Kallikrein 10) ELISA Kit. Human KLK10(Kallikrein 10) ELISA Kit ... Description: KLK10 Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 266 amino ...
The mucin-selective protease StcE enables molecular and functional analysis of human cancer-associated mucins. ... The mucin-selective protease StcE enables molecular and functional analysis of human cancer-associated mucins. ... test kits for ph The mucin-selective protease StcE enables molecular and functional analysis of human cancer-associated mucins. ... test kits for ph The mucin-selective protease StcE enables molecular and functional analysis of human cancer-associated mucins. ...
The mucin-selective protease StcE enables molecular and functional analysis of human cancer-associated mucins. ... test kits for ph The mucin-selective protease StcE enables molecular and functional analysis of human cancer-associated mucins. ... Human CA1(Carbonic Anhydrase I) ELISA Kit. Human CA1(Carbonic Anhydrase I) ELISA Kit ... The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human CA1 . Standards or samples are ...
Mucosal integrity is maintained by the salivary components mucins, electrolytes, and water.12 The properties of mucin include ... Secretory IgA can directly neutralize viruses and mucins can inhibit human immunodeficiency virus (HIV) and herpes virus ... of the total mucin in the salivary secretion.13 The mucin covers the epithelial surfaces of the oral cavity. This results in ... soft tissues of the oral cavity are mucins, proline-rich glycoproteins, and water.12 Mucins are derived from the mucous- ...
  • Mucin-domain glycoproteins are found in nearly every tissue of the human body, and are important in biological processes ranging from embryogenesis to cancer. (pnas.org)
  • Here, we help address a hurdle to the study of mucin-domain glycoproteins by characterizing a bacterial protease with selectivity for mucins. (pnas.org)
  • Mucin-domain glycoproteins are known to be key players in a host of human diseases, especially cancer, wherein mucin expression and glycosylation patterns are altered. (pnas.org)
  • Mucins are highly O-glycosylated glycoproteins ubiquitous in mucous secretions on cell surfaces and in body fluids. (genome.jp)
  • Despite numerous reports on streptococcal adherence to various glycoproteins, no information is available on the interaction of these organisms with mucin, the major glycoprotein component of respiratory tract mucus. (asm.org)
  • Mucus consists of a major glycoprotein, mucin, in addition to several other components such as serum glycoproteins, lipids, and immunogloblulins ( 47 , 56 ). (asm.org)
  • Mucins are complex, carbohydrate-rich glycoproteins secreted by mucosal and submucosal glands and are generally subdivided into two major types, a secretory soluble type and a membrane-bound type (see references 47 and 48 for reviews). (asm.org)
  • Mucin glycoproteins play a key role in the normal function of the airway epithelium. (ox.ac.uk)
  • BACKGROUND AND AIMS: Mucin glycoproteins play a key role in the normal function of the epithelium lining the gastrointestinal tract. (ox.ac.uk)
  • Mucins are described as high molecular weight glycoproteins, which are considered to be the major protein components for the body mucus. (bu.edu)
  • Mucins are high molecular weight glycoproteins with 80% carbohydrates and 20% core protein. (abcam.com)
  • Cloning of partial cDNA encoding differentiation and tumor-associated mucin glycoproteins expressed by human mammary epithelium", Proc. (freepatentsonline.com)
  • MUC5AC (gastric mucin) belongs to the gel-forming mucin family of glycoproteins, the major components of the protective mucus layer on the mucosal surfaces of epithelial tissues. (novusbio.com)
  • This study is an attempt to biochemically characterise gastric mucins or mucus glycoproteins, (the main gel forming components of crude mucus scrapings off the mucosal surface), in carcinoma of the stomach (HCA), as compared to those in ulcer disease (HGU), post mortem specimens (PM) and samples obtained from organ transplant donor stomachs (HD). (nrf.ac.za)
  • There is intense interest in how bacteria interact with mucin glycoproteins in order to colonise mucosal surfaces. (mdpi.com)
  • In this study, we have assessed the feasibility of using recombinant mucin glycoproteins to study the interaction of the gastric pathogen Helicobacter pylori with MUC5AC, a mucin which the organism exhibits a distinct tropism for. (mdpi.com)
  • Mucins are a group of high molecular weight glycoproteins consisting of a mucin core protein and O-linked carbohydrates. (scbt.com)
  • The main structural component of mucus is the mucins, large, heavily glycosylated glycoproteins that form gels when sufficiently concentrated (Allen, 1981a). (springer.com)
  • Aslam, A., Spicer, R. D. and Corfield, A. P. (1997) Biochemical analysis of mucin glycoproteins in paediatric colonic mucus. (springer.com)
  • 1989) Structures of 0-glycosidically linked oligosaccharides isolated from human meconium glycoproteins. (springer.com)
  • This is because many antibodies selected for epithelial or tumor specificity have been found to react with high molecular weight glycoproteins which are produced by simple epithelial cells and have the properties of mucins. (springer.com)
  • S. J. Gendler, J. M Burchell, T. Duhig, D. Lamport, R. White, M. Parker and J. Taylor-Papadimitriou, Cloning of partial cDNA encoding differentiation and tumor-associated mucin glycoproteins expressed by human mammary epithelium, Proc. (springer.com)
  • M. Shimizu and K. Yamauchi, Isolation and characterisation of mucin-like glycoproteins in human milk fat globule membranes. (springer.com)
  • Abnormalities of mucin-type glycoproteins have been described in lung cancers, but their molecular basis is unknown. (elsevier.com)
  • Mucins (MUC) are high-molecular-weight glycoproteins constituted by a protein backbone (apomucin) and carbohydrate side chains. (springer.com)
  • Epithelial mucins are large complex cell surface and secreted glycoproteins produced by mucosal epithelial cells. (springer.com)
  • Eosinophil recruitment and mucus hypersecretion are characteristic of asthmatic airway inflammation, but eosinophils have not been shown to induce mucin production. (jimmunol.org)
  • Human tissues in fatal asthma are reported to show extensive eosinophil degranulation, especially in mucus plugs and in surface epithelium ( 7 ), and a positive correlation between eosinophil counts in bronchoalveolar lavage fluid and sputum production has been reported in asthmatic patients ( 8 ). (jimmunol.org)
  • Recent studies suggest that differences exist in eosinophil effector functions between mice and humans ( 11 , 12 ), so the role of eosinophils in mucus production in humans remains unknown. (jimmunol.org)
  • The present study was undertaken to characterize the interaction between mucin, the major glycoprotein component of mucus, and streptococci. (asm.org)
  • Although some mucins are membrane -bound due to the presence of a hydrophobic membrane-spanning domain that favors retention in the plasma membrane , most mucins are secreted as principal components of mucus by mucous membranes or are secreted to become a component of saliva . (wikipedia.org)
  • Muc5b and Muc5ac are the major oligomeric mucins in equine airway mucus. (semanticscholar.org)
  • Secreted by host goblet cells, mucus is largely composed of highly glycosylated mucin proteins. (nature.com)
  • The two major mucus secreting salivary glands, the sublingual and submandibular glands, are capable of producing mucins although little is known about differences in the type of mucins and amount that might be secreted by each gland. (bu.edu)
  • Therefore, the purpose of our research was to compare the differential expression patterns of the mucins MUC 1, MUC4 , MUC5B , MUC7 and MUC 19 from the two major mucus secreting salivary glands in the oral cavity. (bu.edu)
  • Gastric Mucin 5AC antigen is found in columnar mucus cells of surface gastric epithelium and in goblet cells of the fetal and precancerous colon but not in normal colon. (abcam.com)
  • 1993. Additionally we are shipping Mucin 6, Oligomeric Mucus/gel-Forming Antibodies (99) and Mucin 6, Oligomeric Mucus/gel-Forming Proteins (2) and many more products for this protein. (antibodies-online.com)
  • Mucins were extracted from crude mucus gel scrapings according to a carefully designed technique in which proteolytic inhibitors were used to minimise the possibility of endogenous proteolysis in the laboratory through possible contamination. (nrf.ac.za)
  • Mucin 3 is a major com-ponent of various mucus gels and is broadly expressed in normal and tumor cells. (scbt.com)
  • Mucin 5B Antibody (5B#19-2E) is a high quality monoclonal Mucin 5B antibody (also designated mucin 5B oligomeric mucus/gel-forming antibody or MUC5B antibody) suitable for the detection of the Mucin 5B protein of mouse, rat and human origin. (scbt.com)
  • Native mucus gels contain 2-10% mucin (mucus glycoprotein) dry weight, most of the balance being water. (springer.com)
  • The mucin proteins MUC5AC and MUC5B are the major glycoprotein components of mucus and have critical roles in airway defense. (genetics.org)
  • the genetic architecture of mucus phenotypes in both humans and animal models is not well characterized. (genetics.org)
  • Bacteria colonizing the mucus layer have evolved to use mucin as a carbon and energy source. (asm.org)
  • Current information on the effect of diet on the secretion of mucus and the recovery of mucin in ileal digesta is summarized. (jafs.com.pl)
  • Most important among these is the proteolytic breakdown of mucus gels into component mucin subunits and their subsequent release into the intestinal lumen. (jafs.com.pl)
  • Although regulations of MUC1 and MUC4 mucin genes by bile acids, such as DCA, CDCA and TCA, in human oesophageal cancer cells have been the thorough extensive study [ 14 - 16 ], the mechanisms responsible for regulation of MUC2 expression in the esophageal adenocarcinoma cells remain unknown. (biomedcentral.com)
  • Human intestinal mucin genes MUC3A and MUC3B are members of a membrane mucin gene family residing at chromosome 7q22. (auckland.ac.nz)
  • Mucin genes encode mucin monomers that are synthesized as rod-shape apomucin cores that are post-translationally modified by exceptionally abundant glycosylation . (wikipedia.org)
  • Developmental expression of mucin genes in the human respiratory tract. (ox.ac.uk)
  • We examined the expression of mucin genes, MUC3, 4, 5AC, 5B, 6, 7, and 8 in human fetal tissues to establish the localization and age of onset of expression of each mucin gene during human development. (ox.ac.uk)
  • CONCLUSIONS: Mucin genes are expressed from the early mid-trimester of gestation in the developing human fetal gastrointestinal tract. (ox.ac.uk)
  • The four human mucin genes MUC6, MUC2, MUC5AC, and MUC5B are located at chromosome 11p15.5. (univ-lille.fr)
  • These histological changes were reflected in variable expression of the secretory Muc5AC and low expression of membrane-associated Muc4 mucin genes. (semanticscholar.org)
  • Two novel mucin genes down-regulated in colorectal cancer identified by differential display. (semanticscholar.org)
  • Similar imperfect purine/pyrimidine mirror repeat (PMR) elements have previously been identified upstream of the human MUC1 mucin and CFTR genes. (nebraska.edu)
  • The Mucin 5B gene, which contains a 3′ cis-element, is one of the four mucin genes mapped to human chromosome 11p15. (scbt.com)
  • 1993) Expression of human mucin genes in respiratory, digestive, and reproductive tracts ascertained by in situ hybridization. (springer.com)
  • To identify genes that underlie variation in airway mucin levels, we performed genetic analyses in founder strains and incipient lines of the Collaborative Cross (CC) in a house dust mite mouse model of asthma. (genetics.org)
  • Multiple mucin genes and a diverse range of carbohydrate epitopes were observed with all six cell lines derived from mucinous adenocarcinomas. (elsevier.com)
  • Therefore, ovarian carcinoma cells from tumors of different histological types showed characteristic expression patterns of mucin genes and carbohydrate epitopes. (elsevier.com)
  • An analysis of whole-transcriptome sequencing (RNA-Seq) comparing the gene expression of A. muciniphila grown on mucin with that of the same bacterium grown on glucose confirmed the activity of the genes involved in mucin degradation and revealed most of these to be upregulated in the presence of mucin. (asm.org)
  • In this study, mucin-core-peptide-specific antibodies and cDNA probes were used to determine the relative expression of mucin genes corresponding to one membrane-bound mucin (MUC1), two intestinal mucins (MUC2 and MUC3), and one tracheobronchial mucin (MUC4) in normal (nonneoplastic) lung, and in lung neoplasms. (elsevier.com)
  • Because an epidermal growth factor receptor (EGFR) cascade induces MUC5AC mucin in airways, and because EGFR is up-regulated in asthmatic airways, we examined the effect of eosinophils on MUC5AC mucin production in NCI-H292 cells (a human airway epithelial cell line that produces mucins). (jimmunol.org)
  • Eosinophils were isolated from the peripheral blood of allergic patients, and their effects on MUC5AC mucin gene and protein synthesis were assessed using in situ hybridization and ELISAs. (jimmunol.org)
  • EGF, TGF-α) resulted in MUC5AC mucin gene and protein synthesis in human airway epithelial (NCI-H292) cells in vitro, and selective inhibitors of EGFR tyrosine kinase phosphorylation prevented ligand-induced mucin synthesis in vitro and prevented allergen-induced goblet cell metaplasia in pathogen-free rats ( 13 ). (jimmunol.org)
  • MUC5AC is the main gel-forming mucin expressed by goblet cells of the airways and stomach where it protects the underlying epithelia. (portlandpress.com)
  • We expressed the C-terminal cysteine-rich part of the human MUC5AC mucin in CHO-K1 cells (Chinese-hamster ovary K1 cells) where it formed disulfide-linked dimers in the ER (endoplasmic reticulum). (portlandpress.com)
  • MUC5AC mucin is a component of the human precorneal tear film. (arvojournals.org)
  • The mRNAs of MUC1, MUC4, and MUC5AC have previously been identified in human conjunctiva. (arvojournals.org)
  • The purpose of the this study was to quantify MUC5AC transcript levels, to identify MUC5AC protein in conjunctiva, tears, and goblet cells and to determine whether this mucin is secreted in response to the calcium ionophore ionomycin. (arvojournals.org)
  • METHODS: MUC5AC mRNA from normal human conjunctiva was identified, quantified, and compared with beta2-microglobulin levels using a competitive reverse transcription-polymerase chain reaction (RT-PCR) method. (arvojournals.org)
  • An antibody to a MUC5AC peptide was used to localize this mucin in conjunctival sections by immunohistochemistry. (arvojournals.org)
  • Conjunctival tissues were incubated with ionomycin, and secreted mucins were detected with Helix pomatia agglutinin conjugated to horseradish peroxidase and with anti-MUC5AC antiserum. (arvojournals.org)
  • Ionomycin stimulation of conjunctival secretion resulted in a fourfold increase in total mucin secretion and in a corresponding increase in secreted MUC5AC. (arvojournals.org)
  • CONCLUSIONS: MUC5AC is synthesized by goblet cells of the normal human conjunctiva, and this mucin is a component of conjunctival secretions and of normal human tears. (arvojournals.org)
  • The major secreted airway mucins are MUC5AC and MUC5B , while MUC2 is secreted mostly in the intestine but also in the airway. (wikipedia.org)
  • Genomic organization of the 3'-region of the human MUC5AC mucin gene: additional evidence for a common ancestral gene for the 11p15.5 mucin gene family. (semanticscholar.org)
  • The human mucin gene MUC5AC is mapped clustered with MUC2, MUC5B and MUC6 on chromosome 11p15.5. (semanticscholar.org)
  • It has been demonstrated that the three mucins MUC2, MUC5AC, and MUC5B contain several Cys-subdomains of 108 amino acid residues. (univ-lille.fr)
  • ab77576 is specific for the MUC5AC tandem repeat of human mucin. (abcam.com)
  • Immunohistochemistry-Paraffin: Mucin 5AC Antibody (45M1) - Azide and BSA Free [NBP2-32732] - Formalin-fixed, paraffin-embedded Rat Stomach stained with MUC5AC Monoclonal Antibody (45M1). (novusbio.com)
  • H. pylori bound better to secreted mucin than intracellular mucin suggesting that modifications on extracellular MUC5AC promoted binding. (mdpi.com)
  • In a paper entitled " Genome Reference and Sequence Variation in the Large Repetitive Central Exon of Human MUC5AC ," published in the American Journal of Respiratory Cell and Molecular Biology, corresponding authors Wanda O'Neal and Judith Voynow along with their collaborators describe the use of Single Molecule, Real-Time (SMRT®) Sequencing to characterize a complex mucin exon. (pacb.com)
  • MUC5AC, located on the P arm of chromosome 11, encodes one of the two major secreted mucins found in our airways and contributes to gastrointestinal homeostasis through its activity in the gut. (pacb.com)
  • Analysis of gene and protein expression of Muc5ac and Muc5b in incipient CC lines ( n = 154) suggested that post-transcriptional events were important regulators of mucin protein content in the airways. (genetics.org)
  • Given the importance of MUC5AC and MUC5B in muco-obstructive airway diseases, we sought to identify key regulators of these secreted mucins using an unbiased, genome-wide search. (genetics.org)
  • Seventeen human ovarian carcinoma cell lines were evaluated for their expression of mucin core polypeptide mRNAs (MUC1, MUC2, MUC3, MUC5AC, MUC5B, and MUC6) by the reverse transcription-polymerase chain reaction. (elsevier.com)
  • Expression of mucins (MUC1, MUC2, MUC5AC, and MUC6) and type 1 Lewis antigens in cases with and without Helicobacter pylori colonization in metaplastic glands of the human stomach. (springer.com)
  • Methods Sections were stained with H&E, Alcian blue-periodic acid Schiff (AB-PAS), polyclonal MUC5B antibody, and specific MUC5B riboprobe for histological, histochemical, immunohistochemical, and mucin mRNA analyses. (wiley.com)
  • In situ hybridization and immunohistochemistry studies demonstrated that the secretory cells of the middle ear mucosa with COM expressed MUC5B mucin mRNA and its product MUC5B mucin. (wiley.com)
  • Conclusions The MUC5B mucin gene and its product were identified in the middle ear secretory cells of patients with COM. (wiley.com)
  • MUC5B, the predominate mucin in the mouth and female genital tract, has been shown to significantly reduce attachment and biofilm formation of Streptococcus mutans , a bacteria with the potential to form cavities. (wikipedia.org)
  • [8] Similar effects of MUC5B and other mucins have been demonstrated with other pathogens, such as Candida albicans , Helicobacter pylori , and even HIV . (wikipedia.org)
  • We have shown that MG1 is actually a mixture of mucin gene products, containing MUC4, MUC5B and a novel salivary mucin (NSM), whereas MG2 is a single gene product encoded in the MUC7 gene. (grantome.com)
  • 2. investigate the biosynthesis and assembly of MUC4, MUC5B and NSM using mucin constructs expressed in COS-7 cells. (grantome.com)
  • The expression of a subset of mucins (MUC2, MUC6, MUC5B) was also correlated with sialyl-Tn expression in LS174T cells. (antibodies-online.com)
  • The MUC6 gene encodes gastric mucin, a secreted glycoprotein that plays an essential role in epithelial cytoprotection from acid, proteases, pathogenic microorganisms, and mechanical trauma in the gastrointestinal tract (summary by Toribara et al. (antibodies-online.com)
  • Aberrant mucin glycoprotein patterns of chronic rhinosinusitis patients with bacterial biofilms. (biomedsearch.com)
  • 4) Mucin glycoprotein MUC7 that has known bactericidal activity displays an overall reduced terminal sugar profile in biofilm positive CRS patients. (biomedsearch.com)
  • This study was undertaken to analyze the sera and tissue level detectable mucin-type glycoprotein (TF-antigen) by Peanut agglutinin (PNA) and its diagnostic index in serum as well tissues of human esophageal squamous cell carcinoma as marker. (biomedcentral.com)
  • The glycoprotein Mucin 16 (MUC16) has been previously investigated as a targetable tumor antigen. (sciencemag.org)
  • Here, we describe a human bispecific antibody (REGN4018) that binds both Mucin 16 (MUC16), a glycoprotein that is highly expressed on ovarian cancer cells, and CD3, thus bridging MUC16-expressing cells with CD3 + T cells. (sciencemag.org)
  • H. Sekine, T. Ohno and D. Kufe, Purification and characterization of a high molecular weight glycoprotein detectable in human milk and breast carcinomas, J. Immunol. (springer.com)
  • This sequence is also found in the human MUC2 mucin, previously shown to be cleaved at the same site by a slow, non-enzymatic process triggered by a pH below 6 [Lidell, Johansson and Hansson (2003) J. Biol. (portlandpress.com)
  • Mucin alterations are a common feature of esophageal neoplasia, and alterations in MUC2 mucin have been associated with tumor progression in the esophagus. (biomedcentral.com)
  • Deoxycholic acid induced MUC2 overexpression in human esophageal adenocarcinoma cells by activation of Nuclear factor-κB transcription through a process involving PKC-dependent but not PKA, independent of activation of MAP kinase. (biomedcentral.com)
  • We find that bile acids induce MUC2 expression in human esophageal adenocarcinoma cells at the level of transcription through a process that involves protein kinase C (PKC)-dependent activation of Nuclear factor-κB (NF-κB), primarily a MAP kinase-independent. (biomedcentral.com)
  • Ajoika, Y., Allison, L. J. and Jass, J. R. (1996) Significance of MUC1 and MUC2 mucin expression in colorectal cancer. (springer.com)
  • Mucin-associated carbohydrate epitopes were not detected on three of four serous adenocarcinoma cell lines, although MUC1 and MUC2 mRNAs were detected in all of them. (elsevier.com)
  • Normal lung tissues exhibited a distinct pattern of mucin gene expression, with high levels of MUC1 and MUC4 mRNA and low to absent levels of MUC2 and MUC3 mucin immunoreactivity and mRNA. (elsevier.com)
  • Two membrane mucins, MUC1 and MUC4 have been extensively studied in relation to their pathological implication in the disease process. (wikipedia.org)
  • determine the structural organization and sequence of mucin gene products comprising MG1 by characterization of a novel salivary mucin (NSM) and elucidating the C-terminal sequence of MUC4. (grantome.com)
  • Alternative splicing generates a family of putative secreted and membrane-associated MUC4 mucins. (semanticscholar.org)
  • Retinoic acid-dependent transforming growth factor-beta 2-mediated induction of MUC4 mucin expression in human pancreatic tumor cells follows retinoic acid receptor-alpha signaling pathway. (semanticscholar.org)
  • Complete sequence of the human mucin MUC4: a putative cell membrane-associated mucin. (semanticscholar.org)
  • METHODS: Human corneal epithelial cells were cultured in medium supplemented with various concentrations of dexamethasone (ranging from 10 to 10 M). Reverse transcription-polymerase chain reaction (RT-PCR) and Western blot analysis using monoclonal antibodies specific for human MUC1 (HMFG-1), MUC4 (1G8), and MUC16 (OC125) were performed to evaluate the effect of dexamethasone on membrane-associated mucin expression. (yonsei.ac.kr)
  • CONCLUSIONS: This study shows that MUC1, MUC4, and MUC16 are regulated differently by dexamethasone in human corneal epithelial cells. (yonsei.ac.kr)
  • Lung squamous-cell, adenosquamous, and large-cell carcinomas were characterized by increased levels of MUC4 mucin only. (elsevier.com)
  • The frequent occurrence of increased MUC4 transcripts in a variety of non-small-cell lung cancers indicates the potential importance of this type of mucin in lung cancer biology. (elsevier.com)
  • The MUC4 mucin is a high molecular weight, membrane-bound, and highly glycosylated protein. (nebraska.edu)
  • It is a multi-domain protein that is putatively cleaved into a large mucin-like subunit (MUC4α) and a C-terminal growth-factor like subunit (MUC4β). (nebraska.edu)
  • Further, MUC4 plays diverse functional roles in cancer initiation and progression as evident from its involvement in oncogenic transformation, proliferation, inhibition of apoptosis, motility and invasion, and resistance to chemotherapy in human cancer cells. (nebraska.edu)
  • Three anti MUC4α-N-Ter and one anti-MUC4α-C-Ter antibodies were characterized by several inmmunoassays including enzyme-linked immunosorbent assay (ELISA), immunoblotting, immunofluorescene, flow cytometry and immunoprecipitation using MUC4 expressing human pancreatic cancer cell lines. (nebraska.edu)
  • The antibodies also reacted with the MUC4 in human pancreatic tumor sections in immunohistochemical analysis. (nebraska.edu)
  • This review focuses on the glycosyltransferases of colon and breast cancer cells that determine the pathways of mucin-type O-glycosylation, and the proposed functional and pathological consequences of altered O-glycans. (nih.gov)
  • In order to define which glycans may be implicated in the binding of the bacteria to the gastric mucosa in humans, we have characterized the exact pattern of glycosylation of gastric mucins. (pasteur.fr)
  • We have also demonstrated that around 80% of O-glycans carried blood group A, B or H antigens, suggesting that the variation of gastric mucin glycosylation between individuals is partly due to the blood group status. (pasteur.fr)
  • Resurgence of gastric mucin during colonic carcinogenesis is suggestive of either re-expression of the peptide core of gastric mucin in the adult colon or due to changes in the glycosylation pattern of mucin, which expose the hidden Mucin 5AC antigen. (abcam.com)
  • The present study reveals that, during tumorigenesis, an aberrant glycosylation takes place in Golgi apparatus leading to over secretion of TF antigen into the cytoplasm along with mucin granules and later into cell membrane. (biomedcentral.com)
  • The vast majority of mucin glycosylation is constituted by O -glycans which are linked to serine (Ser) or threonine (Thr) residues on the protein backbone, and, in fact, more than 50% of the mucin molecular mass is composed of carbohydrates. (springer.com)
  • Mucin domains are densely O -glycosylated modular protein domains that are found in a wide variety of cell surface and secreted proteins. (pnas.org)
  • Western blots probed with 125 I-labeled mucin and a panel of monoclonal antibodies revealed that the streptococcal M protein is one of two cell wall-associated proteins responsible for this binding. (asm.org)
  • This observation is in accordance with recent studies showing that surface proteins of other lactobacilli participate in adhesion to epithelial cell lines, gastrointestinal mucins, or extracellular matrix proteins ( 1 , 26 , 58 , 60 ). (asm.org)
  • Mucins ( / ˈ m juː s ɪ n / ) are a family of high molecular weight , heavily glycosylated proteins ( glycoconjugates ) produced by epithelial tissues in most animals . (wikipedia.org)
  • Overexpression of the mucin proteins, especially MUC1 , is associated with many types of cancer. (wikipedia.org)
  • [9] [10] In the mouth, mucins can also recruit anti-microbial proteins such as statherins and histatine 1 , which further reduces risk of infection. (wikipedia.org)
  • Mucins are secreted as massive aggregates of proteins with molecular masses of roughly 1 to 10 million Da . (wikipedia.org)
  • The mucins are a family of highly glycosylated, secreted proteins with a basic structure consisting of a variable number of tandem repeats (VNTRs) encoded by 60 base pairs (Mucin 1), 69 base pairs (Mucin 2) and 51 base pairs (Mucin 3). (scbt.com)
  • Mucin 1 proteins are expressed as type I membrane proteins in addition to secreted forms. (scbt.com)
  • Mucin domains are densely O -glycosylated modular protein domains which might be present in all kinds of cell floor and secreted proteins . (novosides.eu)
  • Mucins are glycoconjugated proteins which are secreted by vesicles and discharged on the surface of the conjunctival epithelium of the eye. (google.com)
  • In the case of contact lenses, mucins are often viewed as a debris that, like other proteins, should not accumulate on the contact lens surface. (google.com)
  • Following cell fusion, hybridomas were screened using the aforementioned recombinant proteins ad lysates from human pancreatic cell lines. (nebraska.edu)
  • Abstract--"Mucin Based Immunity and Immunotherapy of Cancer", by O.J. Finn, Dept. of Molecular Genetics and Biochemistry, University of Pittsburgh School of Medicine, Pittsburgh, PA 15261, USA. (freepatentsonline.com)
  • Association of ulcerative colitis with rare VNTR alleles of the human intestinal mucin gene, MUC3. (ox.ac.uk)
  • Recently, evidence of linkage on chromosome 7q near the intestinal mucin gene MUC3 was reported by an affected sib-pair analysis. (ox.ac.uk)
  • 2. Considerable heterogeneity was found with both antibody and lectin typing, but there was no significant difference in heterogeneity between mucin from patients with gallstones and control subjects. (clinsci.org)
  • All mucin preparations reacting with the polyclonal antibody also bound to Helix pomatia agglutinin. (clinsci.org)
  • There are currently no images for Mucin 5AC Antibody (NBP2-34758V). (novusbio.com)
  • Antibody to gastric mucin M1 reacts with the gastric epithelium of normal human gastrointestinal tract as well as with the precancerous and cancerous colon but not with normal adult colon. (novusbio.com)
  • Immunohistochemistry-Paraffin: Mucin 5AC Antibody (45M1) [Azide Free] [NBP2-32732] - Human stomach. (novusbio.com)
  • Mucin 3 Antibody (M3.1) is a high quality monoclonal Mucin 3 antibody (also designated mucin 3A, cell surface associated antibody or MUC3A antibody) suitable for the detection of the Mucin 3 protein of human origin. (scbt.com)
  • Mucin 3 Antibody (M3.1) is available as the non-conjugated anti-Mucin 3 antibody. (scbt.com)
  • I am using Mucin 3 (M3.1): sc-7315 monoclonal antibody to stain paraffin embedded tissue. (scbt.com)
  • m-IgG Fc BP-HRP (mouse IgG Fc binding protein-HRP) and 1 BP-HRP">m-IgG 1 BP-HRP (mouse IgG 1 binding protein-HRP) are the preferred secondary detection reagents for Mucin 5B Antibody (5B#19-2E) for WB applications. (scbt.com)
  • These reagents are now offered in bundles with Mucin 5B Antibody (5B#19-2E) ( see ordering information below ). (scbt.com)
  • Mucin 5B Antibody (5B#19-2E) is available as both the non-conjugated anti-Mucin 5B antibody form, as well as multiple conjugated forms of anti-Mucin 5B antibody, including agarose, PE, FITC and multiple Alexa Fluor ® conjugates. (scbt.com)
  • Moreover, in a genetically engineered immunocompetent mouse expressing human CD3 and human MUC16 [humanized target (HuT) mice], REGN4018 inhibited growth of murine tumors expressing human MUC16, and combination with an anti-PD-1 antibody enhanced this efficacy. (sciencemag.org)
  • Effective mucin dispersion into ASL requires salt and water secretion onto the mucosal surface, but how mucin secretion rate is coordinated with ion and, ultimately, water transport rates is poorly understood. (nih.gov)
  • Objectives To identify the mucin gene and its expressing cells in the middle ear mucosa with chronic otitis media (COM), and to study the correlation between infiltration of inflammatory cells in the submucosa and expression of the mucin gene in the mucosal epithelium with COM. (wiley.com)
  • The dense "sugar coating" of mucins gives them considerable water-holding capacity and also makes them resistant to proteolysis , which may be important in maintaining mucosal barriers. (wikipedia.org)
  • Mucins are found on moist, mucosal epithelia, and are thought to combine mechanical protection of eye tissue as well as chemical and immune protection of mucosal tissue. (google.com)
  • In the case of eye drop solutions, the cationic polysaccharides, after binding to the mucosal eye tissue, may in turn promote the mucins in the eye, either by supplementing the mucin and/or by helping to bind and maintain mucin on the eye surface. (google.com)
  • IMPORTANCE Akkermansia muciniphila is among the most abundant mucosal bacteria in humans and in a wide range of other animals. (asm.org)
  • Authoritative and practical, Mucins: Methods and Protocols is designed to be a useful resource for those entering the mucin field and to facilitate those already studying mucins to broaden their experimental approaches to understanding mucosal biology. (springer.com)
  • The mucous secretions produced by some epithelial cells, particularly those lining the gastro-intestinal tract and the lungs, contain mucins along with other products and these components have been studied for some time at the biochemical level. (springer.com)
  • These results show that activated eosinophils induce mucin synthesis in human airway epithelial cells via EGFR activation, and they implicate TGF-α produced by eosinophils and epithelial cells in the EGFR activation that results in mucin production in human airway epithelium. (jimmunol.org)
  • Mucins anchor this tear film to the epithelium and protect the eye surface from bacterial, chemical and physical invasion of foreign bodies. (google.com)
  • For this purpose polyclonal and monoclonal antibodies against gallbladder mucin were raised. (clinsci.org)
  • Monoclonal antibodies specific for human polymorphic epithelial mucin. (freepatentsonline.com)
  • J. Burchell, H. Durbin and J. Taylor-Papadimitriou, Complexity of expression of antigenic determinants recognised by monoclonal antibodies HMFG-1 and HMFG-2, in normal and malignant human mammary epithelial cells, J. Immunol. (springer.com)
  • J. Burchell, S. Gendler, J. Taylor-Papadimitriou, A. Girling, A. Lewis, R. Millis and D. Lamport, Development and characterization of breast cancer reactive monoclonal antibodies directed to the core protein of the human milk mucin, Cancer Res. (springer.com)
  • C. Foster, P.A. Edwards, E.A. Dinsdale and A.M. Neville, Monoclonal antibodies to the human mammary gland. (springer.com)
  • R. L. Ceriani, J. A. Peterson, J. Y. Lee, R. Moncada and E. W. Blank, Characterisation of cell surface antigens of human mammary epithelial cells with monoclonal antibodies prepared against human milk fat globule, Som. (springer.com)
  • Resurgence of gastric mucin reactivity during colonic carcinogenesis is due to re-expression of the peptide core of gastric (or fetal colonic) mucins. (novusbio.com)
  • Mucin 2 coats the epithelia of the intestines and airways and is associated with colonic tumors. (scbt.com)
  • 1992) Oligosaccharide structures of mucins secreted by the human colonic cancer cell line CL.16E. (springer.com)
  • This MAb recognizes the peptide core of gastric mucin M1 (>1,000kDa) (recently identified as Mucin 5AC). (novusbio.com)
  • 1991) Evidence for different human tracheobronchial mucin peptides deduced from nucleotide cDNA sequences. (springer.com)
  • Nineteen of the 21 reacting mucins (90%) were from patients with blood group A (18 patients) or AB (one patient) and expression of A antigen could be demonstrated on the mucin of these patients. (clinsci.org)
  • The resulting two reacting mucins were from patients with type O. However, expression of the blood group antigen could not account for the lack of reactivity of the mucin of other patients. (clinsci.org)
  • Bile acids have been linked to esophageal adenocarcinoma and mucin secretion, but their effects on mucin gene expression in human esophageal adenocarcinoma cells is unknown. (biomedcentral.com)
  • Certain microorganisms and disease states may alter mucin biochemistry or expression. (ajtmh.org)
  • METHODS: Mucin gene expression was assayed by mRNA in situ hybridisation. (ox.ac.uk)
  • The authors propose that the expression of TF-antigen in human may play an important role during tumorigenesis establishing it as a chemically well-defined carcinoma-associated antigen. (biomedcentral.com)
  • Mucous differentiation and expression of Mucin 5B is retinoic acid- (RA) or retinol-dependent. (scbt.com)
  • Bobeck, L. A., Tsai, H., Biesbrock, A. R. and Levine, M. J. (1993) Molecular cloning, sequence and specificity of expression of the gene encoding the low molecular weight human salivary mucin (MUC7). (springer.com)
  • Specific immunotherapy by the use of mucins or carbohydrate vaccines has been attempted, in which expression of these epitopes by each histotype should clearly be assessed. (elsevier.com)
  • They were also examined for their expression of mucin-associated carbohydrate epitopes by flow cytometry. (elsevier.com)
  • The effect of glucocorticoid receptor antagonist (RU38486) on dexamethasone-induced mucin expression was estimated. (yonsei.ac.kr)
  • thus, the effect of dexamethasone on mucin expression is mediated by glucocorticoid receptors. (yonsei.ac.kr)
  • We conclude that the expression of one membrane-bound and several secretory-type mucins is independently regulated and markedly altered in lung neoplasms. (elsevier.com)
  • MUC6 apomucin shows a distinct normal tissue distribution that correlates with Lewis antigen expression in the human stomach. (springer.com)
  • HER2/neu-derived Peptides Are Shared Antigens among Human Non-Small Cell Lung Cancer and Ovarian Cancer", Cancer Research 54 3387-3390, Jul. (freepatentsonline.com)
  • Humans carry either a five or six tandem repeat version of the gene. (wikipedia.org)
  • Some modern people from Sub-Saharan Africa were found to carry a five tandem repeat variation of the MUC7 gene that is highly divergent from other, modern humans. (wikipedia.org)
  • Humoral Immunity against a Tandem Repeat Epitope of Human Mucin MUC-1 in Sera from Breast, Pancreatic, and Colon Cancer Patients.sup.1, Advances in Brief, Cancer Research 54, 2856-2860, Jun. (freepatentsonline.com)
  • This study demonstrates the feasibility of using recombinant mucins containing tandem repeat sequences to assess microbial mucin interactions. (mdpi.com)
  • Mucins share a common structural feature which is a tandem repeat domain comprising a variable number of tandem repeats (VNTRs) of identical or highly similar sequences rich. (springer.com)
  • Mucin-7 is a protein that in humans is encoded by the MUC7 gene. (wikipedia.org)
  • The coalescence age for the human variations of the MUC7 gene is estimated to be around 4.5 million years ago, which predates the human-Neanderthal split. (wikipedia.org)
  • Sepharose 4B gel chromatography, on the other hand, revealed that this degradation of the mucins was most extensive in the HCA group and decreased in order in the HGU, PH and HO groups. (nrf.ac.za)
  • The results indicated that mucins in gastric cancer, though different from those in other groups by virtue of their greater degradation, were similar to mucins from the ulcerated stomachs in that they co-fractionated with an albumin-carbohydrate complex in a caesium chloride gradient. (nrf.ac.za)
  • Mucin biology has been difficult to study at the molecular level, in part, because methods to manipulate and structurally characterize mucin domains are lacking. (pnas.org)
  • Mucin biology has been tough to check on the molecular stage, partially, as a result of strategies to govern and structurally characterize mucin domains are missing. (novosides.eu)
  • [6] A recently identified human mucin gene is MUC21 , previously known as chromosome 6 open reading frame 205. (wikipedia.org)
  • Neither mucin secretion nor ATP release from Calu-3 cells were affected by activation or inhibition of the cystic fibrosis transmembrane conductance regulator. (nih.gov)
  • Mucins are also overexpressed in lung diseases such as asthma , bronchitis , chronic obstructive pulmonary disease (COPD) or cystic fibrosis . (wikipedia.org)
  • After the preliminary characterization of their phenotype and tumor specificity, detailed studies performed with one of the cell lines, W.D., show that it recognizes a specific antigen, a large and heavily glycosylated mucin molecule, expressed on pancreatic and breast tumors and tumor cell lines. (pnas.org)
  • These findings are important because they demonstrate a specific T-cell response against a human tumor-associated antigen. (pnas.org)
  • Tumor Necrosis Factor Increases MUC1 mRNA in Cultured Human Nasal Epithelial Cells. (semanticscholar.org)
  • S. Gendler, J. Taylor-Papadimitriou, T. Duhig, J. Rothbard, and J. Burchell, A highly immunogenic region of a human polymorphic epithelial mucin expressed by carcinomas is made up of tandem repeats, J. Biol. (springer.com)
  • Mucin production in airways is induced by an epidermal growth factor receptor (EGFR) 3 cascade ( 13 ). (jimmunol.org)
  • 2006). "Characterization of human mucin MUC17. (wikipedia.org)
  • Biophysical Characterization of One-, Two-, and Three-Tandem Repeats of Human Mucin (muc-1) Protein Core.sup.1, Cancer Research 53, 5386-5394, Nov. 15, 1993. (freepatentsonline.com)
  • Purification and characterization of a human pancreatic adenocarcinoma mucin. (semanticscholar.org)
  • We exploited StcE's unique properties to improve sequence coverage, glycosite mapping, and glycoform analysis of recombinant human mucins by mass spectrometry. (pnas.org)
  • Using recombinant La1 EF-Tu protein, we were able to determine that its binding to intestinal cells and to mucins is pH dependent. (asm.org)
  • Recombinant fragment within Human Mucin 5AC aa 2300-2500. (abcam.com)
  • The interaction between microbes and mucins involves mucin carbohydrate side chains and microbial adhesin molecules. (ajtmh.org)
  • In both mucin glycans and HMOs, the priming carbohydrate structure is extended with galactose- N- acetylglucosamine disaccharides and chains often terminate with fucose or sialic acid residues [ 7 ]. (nature.com)
  • When grown on mucin medium, cells produced a capsule and were found to aggregate. (nih.gov)
  • Upon stimulation, MARCKS (myristylated alanine-rich C kinase substrate) protein coordinates the secretion of mucin from mucin-filled vesicles within the specialized epithelial cells. (wikipedia.org)
  • Mucins are secreted by epithelial surfaces that line the reproductive , respiratory, gastrointestinal tracts. (bu.edu)
  • N-linked oligosaccharides are also found on mucins, but in less abundance than O-linked sugars. (wikipedia.org)
  • These substrates are often derived from diet, such as complex polysaccharides from plants or human milk oligosaccharides (HMOs) during breastfeeding. (nature.com)
  • 1. Human gallbladder mucin has been implicated to play a role in gallstone disease. (clinsci.org)
  • In spite of this fact relatively little is known about the structure of human gallbladder mucin. (clinsci.org)
  • With these antibodies the immunoreactivity of gallbladder mucin from 60 patients with cholesterol gallstones and 20 subjects without stones was screened. (clinsci.org)
  • 3. We conclude that considerable patient to patient heterogeneity of human gallbladder mucin exists. (clinsci.org)
  • Although Mucin 5B is the prominent human gallbladder mucin, it is also expressed and secreted in the colon. (scbt.com)
  • In particular, the mucin-type O-glycans have several cancer-associated structures, including the T and Tn antigens, and certain Lewis antigens. (nih.gov)
  • Cancer-associated mucin antigens can be exploited in diagnosis and prognosis, and in the development of cancer vaccines. (nih.gov)
  • Our data suggest that nucleotide release is a mechanism by which mucin-secreting goblet cells produce paracrine signals for mucin hydration within the ASL. (nih.gov)
  • Mucins protect oral surfaces against desiccation, form a selectively permeable diffusion barrier between underlying surfaces and the external environment, lubricate hard and soft tissues to minimize mechanical injury, facilitate speech and swallowing and provide a physical barrier to protect against colonization of potentially harmful microbes and viruses. (grantome.com)
  • Mucin 5B is abnormally expressed in gastric carcinomatous tissues. (scbt.com)
  • A human tracheobronchial lambda gt 11 cDNA library was screened using antiserum prepared against the deglycosylated protein backbone of human tracheobronchial mucins. (biochemj.org)
  • Two cDNAs, designated JER 28 and 57, obtained from this immunoscreening, were used to isolate two other cDNA clones, JUL 7 and JUL 10, from a human tracheobronchial lambda gt 10 cDNA library. (biochemj.org)
  • The development of mucin-secreting cell lines has facilitated the study of mucin biology and aided our understanding of mucin-microbial interactions. (ajtmh.org)
  • Once in the large intestine mucin is fermented by resident microbial populations. (jafs.com.pl)
  • HMOs are indigestible by humans and are degraded throughout the gastrointestinal tract of breast-fed infants [ 21 ], becoming the primary microbiota-accessible carbohydrates available in the newborn diet. (nature.com)
  • The composition and activity of the microbiota in the human gastrointestinal tract are primarily shaped by nutrients derived from either food or the host. (asm.org)
  • It remains to be determined whether other members of the 7q22 membrane mucin gene family have amino-terminal domains similar to MUC3A and MUC3B. (auckland.ac.nz)
  • [11] Fusion of the vesicles to the plasma membrane causes release of the mucin, which as it exchanges Ca 2+ for Na + expands up to 600 fold. (wikipedia.org)
  • Membrane mucins, such as MUC17, function in epithelial cells to provide cytoprotection, maintain luminal structure, provide signal transduction, and confer antiadhesive properties upon cancer cells that lose their apical/basal polarization. (wikipedia.org)
  • 2008). "The C-terminus of the transmembrane mucin MUC17 binds to the scaffold protein PDZK1 that stably localizes it to the enterocyte apical membrane in the small intestine" (PDF). (wikipedia.org)
  • PURPOSE: To study the influence of dexamethasone on membrane-associated mucins produced by human corneal epithelial cells. (yonsei.ac.kr)
  • There are two main classes of mucins: membrane-bound mucins, contributing to the composition of the cellular glycocalyx, and secreted mucins, contributing to the composition of the extracellular matrix or the mucous barrier that covers all epithelial surfaces in the human body, except the skin. (springer.com)
  • In addition to pancreatic and breast tumors, various mucin molecules are known to be produced by other tumors of epithelial cell origin and could be expected to stimulate similar T-cell-mediated immune responses. (pnas.org)
  • Further analysis revealed that the M protein binds to the sialic acid moieties on mucin, and this interaction seems to be based on M-protein conformation rather than specific amino acid sequences. (asm.org)
  • Human TTF2 is a lectin that binds alpha-GlcNAc-capped mucin 6 g with antibiotic activity against Helicobacter pylori. (antibodies-online.com)
  • Evolutionary history of the 11p15 human mucin gene family. (univ-lille.fr)