Etrolizumab

Motilin receptor

ERCC1

ZP3

Theodore Puck

Nociceptin receptor

FANCG

ERCC4

Proxyfan

VUF-5681

ST6GALNAC1

Granulocyte colony-stimulating factor

Relaxin/insulin-like family peptide receptor 3

Pindolol

Chemically defined medium

CDC2L1

Pertuzumab

B4GALNT1

Doxepin

Sertraline

Dosulepin

Etoperidone

Imipramine

Muscarinic antagonist

Nortriptyline

5-HT2B receptor

Meta-Chlorophenylpiperazine

Ro60-0175

ORG-12962

Robin Holliday

Type 2 diabetes

Positron emission tomography

Zygosity

Faktor aktivacije B-ćelija

Tonsil

ውክፔዲያ - ኣበራ ሞላ

Hypervitaminosis A

PSEN1

Hypothalamic-pituitary-adrenal axis

Acute inhalation injury

Autocrine signalling

L-xylulose reductase

Nociceptinski receptor - Vikipedija, slobodna enciklopedija

Spatial memory

Interleukin 17

Lithium, den frie encyklopædi

Retinoid

2009 United Nations Climate Change Conference

PRNP

Abscess

AKR1B1

Probiotic

Tuberkulóza - Wikipedie

Aminoacyl tRNA synthetase

Harold E. Varmus

氧气中毒 - 维基百科，自由的百科全书

سرطان پروستات - ویکی‌پدیا، دانشنامهٔ آزاد

Crenolanib

Hepatocyte growth factor

Receptor2

• We performed a structure-activity study with the human motilin receptor, which was recently cloned from thyroid tissue. (nih.gov)
• N-terminal fragments, Ala-analogs of motilin, and motilides were tested in a cell line that expresses the cloned human motilin receptor and apoaequorin. (nih.gov)

Amplified Chinese hamst1

• We previously established a ras-oncogene amplified Chinese hamster ovary (CHO) cell line, named ras clone I, as an universal host cell line for oncogene activated production (OAP) system to mass-produce recombinant protein by activation of the cytomegalovirus immediate early (CMV) promoter with ras protein. (elsevier.com)

Expressed in Chinese hamst2

• The study examined 5-HT activation of human 5-HT 1B receptors expressed in Chinese hamster ovary (CHO) cells. (bio-medicine.org)
• As these effects could involve the modulation of repolarizing currents, we assessed effects of H 2 O 2 on HERG (which encodes the cardiac potassium channel I Kr ) expressed in Chinese hamster ovary cells. (aspetjournals.org)

Assay9

• This study investigates the use of FlashPlate as a platform for the measurement of G-protein activation by means of an assay of [ 35 S]-GTPγS binding in Chinese hamster ovary cells. (bio-medicine.org)
• In the aequorin assay, cells co-expressing apoaequorin and the target receptor are first incubated with the co-factor coelenterazinein order to reconstitute the activea equorin enzyme. (perkinelmer.co.uk)
• Aequorin and Photina cell lines - the alternative calcium flux assay. (perkinelmer.co.uk)
• We were able to analyze the expressed and purified products (glycosylated cell-free EPO runs at 25-28 kDa, and unglycosylated protein runs at 20 kDa on an SDS-PAGE), identifying the presence of glycan moieties by PNGase shift assay. (mdsoar.org)
• Additionally, we tested the presence and absence of sugars on the cell-free EPO using a lectin-based assay system. (mdsoar.org)
• Then, the biological activity of rhGH was assessed by a gene reporter assay system [containing LHRE, TK promoter and Luc gene], using HEK 293 cells transfected with GH receptor and response element for STAT-5 measuring luciferase activity on a Berthold luminometer. (bvsalud.org)
• The results of gene reporter assay showed that rhGH produced by CHO cells is able to induce GH intracellular cell signaling . (bvsalud.org)
• Expression of rhIL-7 in CHO-K1 cells was confirmed by enzyme-linked immunosorbent assay (ELISA) and dot and western blots. (rbmb.net)
• Analysis Note: The biological activty is measured in a serum-free cell proliferation assay using the human breast carcinoma cell line, MCF-7. (thomassci.com)

Liter of cultured CHO cells2

• liter of cultured CHO cells, thereby making it possible to produce Alpha-1-antitrypsin from CHO cells at a scale that can eliminate the need for human donors in the future. (dtu.dk)
• This unique method resulted in production of a cell line capable of producing 1.2 grams per liter of cultured CHO cells. (alpha1.uk)

Proteins24

• Researchers from DTU Biosustain have successfully produced mammalian cell lines (CHO) that can produce 1.2 g/L recombinant Alpha-1-antitrypsin proteins with human glycosylation profiles. (dtu.dk)
• The researchers have successfully produced Alpha-1-antitrypsin in CHO cells - one of the most deployed cell lines for production of human therapeutic proteins. (dtu.dk)
• The researchers obtained this glycosylation profile by introducing a human gene encoding an enzyme capable of decorating the sugar backbones on the Alpha-1-antitrypsin proteins with the correct human moieties. (dtu.dk)
• Hence, this new knowledge about glycosylation profiles opens a new window for optimizing human proteins to improve their stability and function. (dtu.dk)
• Cell-Free Protein Synthesis (CFPS) offers many advantages for the production of recombinant therapeutic proteins using the HO cell-free system. (mdsoar.org)
• The researchers found a method to produce Alpha-1-antitrypsin in CHO cells which is the preferred cell line for producing human therapeutic proteins. (alpha1.uk)
• Our data imply that ras clone I is available as a general host cell for establishing the recombinant protein hyper-producing CHO cells by the OAP system, and suggest that further mass production of recombinant proteins in the OAP system can be possible by clarifying the accurate role of upregulated BiP protein. (elsevier.com)
• Cultivated mammalian cells , because of their capacity for proper protein folding , assembly and post-translational modification , have become the dominant system for production of recombinant proteins in clinical application. (bvsalud.org)
• The most generally used mammalian hosts for the production of recombinant proteins are CHO and HEK293 cells. (kowsarpub.com)
• In conclusion, transient transfection studies with HEK cells provide a simple and strong method for a high production of recombinant proteins in weeks, a process which is more time consuming in the CHO cell line. (kowsarpub.com)
• Among them, mammalian expression systems are the main expression systems for the production of recombinant human proteins. (kowsarpub.com)
• Integrins are heterodimeric integral membrane proteins composed of an alpha subunit and a beta subunit that function in cell surface adhesion and signaling. (nih.gov)
• Pretreatment of the cells with pertussis toxin, but not cholera toxin, abolished the (−)-U50,488H-induced increase in [ 35 S]GTPγS binding, which indicates the involvement of G i and/or G o proteins. (aspetjournals.org)
• Cetuximab fostered an ER stress response and the translocation of ER proteins to the cell membrane. (selleckchem.com)
• The goal of animal cell technology includes accomplishments of clonal expansion of differentiated cells with useful ability, optimisation of their culture conditions, modulation of their ability for production of medically and pharmaceutically important proteins, and the application of animal cells to gene therapy, artificial organs, and functional foods. (springer.com)
• Fusion proteins of the invention include a junction region having an amino acid change that reduces the ability of a junctional epitope to bind to MHC Class II, thereby reducing its interaction with a T-cell. (google.ca)
• More specifically, the invention relates to fusion proteins, made less immunogenic by identifying candidate T-cell epitopes and modifying the amino acid sequence to eliminate such epitopes. (google.ca)
• Many therapeutic proteins are normal human proteins. (google.ca)
• For example, interleukin-2, erythropoietin, and growth hormone are all human proteins that are given to humans who already usually make endogenous levels of these proteins. (google.ca)
• In general, immune responses against completely normal human proteins are rare when these proteins are used as therapeutics. (google.ca)
• These proteins include an antibody moiety and a cytokine moiety, and are useful for targeting cytokines to diseased cells, such as cancer cells. (google.ca)
• The BIOSTAT STR® can be connected to different cell retention devices, to retain viable cells in culture for increased space-time yields or to remove sensitive proteins for immediate purification. (sartorius.com)
• Tumor Necrosis Factor Binding Protein I (TBP-I), precursors and analogs thereof, are produced by transfecting eukaryotic cells with an expression vector comprising al DNA molecule encoding the whole human type I TNF receptor or a soluble domain thereof, and culturing the transfected cells, whereby the soluble proteins are secreted into the medium. (google.com)
• Abgent has over fifteen years of experience producing recombinant proteins in E. coli and mammalian cells (CHO and HEK293, etc), and we have added a powerful yeast expression platform to our menu of services. (abgent.com)

Gene25

• CD47 (Cluster of Differentiation 47) also known as integrin associated protein (IAP) is a transmembrane protein that in humans is encoded by the CD47 gene. (genscript.com)
• It is an example of a cell line transfected using our proprietary CBTGS gene screening and amplification system. (creativebiomart.net)
• The human CLCN1 gene encodes the pore-forming subunit of a voltage-gated chloride channel highly expressed in skeletal muscle. (creativebiomart.net)
• 1. Gene expression: qPCR experiments determined specific over-expression of human CLCN1.2. (creativebiomart.net)
• The human TRPM4 gene encodes the pore-forming subunit of TRPM4, a non-selective, cation-permeable channel expressed in lymphocytes, heart, vascular smooth muscle, intestine, and prostate. (creativebiomart.net)
• The human KCNMA1 gene encodes the pore-forming subunit of KCa1.1, a calcium-activated BK potassium channel. (creative-biogene.com)
• Its gene expression is lost in 90% of human pancreatic adenocarcinomas while SSTR2 expression in neuroblastoma correlates positively to overall and event-free survival. (innoget.com)
• The human HCN2 gene encodes the pore-forming subunit of a hyperpolarization-activated, cyclic nucleotide-modulated cation channel. (creative-bioarray.com)
• XRCC9 is a candidate tumor suppressor gene that might operate in a postreplication repair or a cell cycle checkpoint function. (elsevier.com)
• Thompson, Larry H. / The human XRCC9 gene corrects chromosomal instability and mutagen sensitivities in CHO UV40 cells . (elsevier.com)
• Haploid cells are a powerful genetic tool to analyze gene function. (news-medical.net)
• The researchers introduced a human gene which decorated the desired human moieties on the sugar backbones of Alpha-1-antitrypsin protein. (alpha1.uk)
• Recombinant Chinese hamster ovary (rCHO) cell lines expressing a high level (7 ㎍/mL) of recombinant human Ang4 protein (rhAng4) with an amino-terminal FLAG-tag was constructed by transfecting the expression vectors into dihydrofolate reductase (dhfr)-deficient CHO cells and the subsequent gene amplification in medium containing stepwise increments in methotrexate level such as 0.02, 0.08, and 0.32 μM. (kaist.ac.kr)
• We generated a C5λ hyper-producing cell line by transfecting ras clone I with the C5λ gene expression plasmid regulated by the CMV promoter, of which productivity was 5.3 times greater than the hyper productive CHO cell line generated by using conventional CHO cells. (elsevier.com)
• Introduction of the adenovirus E1A gene into the hyper-producing cell line derived from ras clone I resulted in further 9.5 times enhancement of the productivity, suggesting the synergistic effect of E1A and ras oncogenes on the recombinant protein production driven by the CMV promoter. (elsevier.com)
• CHO Versus HEK Cells, Gene Cell Tissue. (kowsarpub.com)
• Lymphocyte-activation gene 3, also known as LAG-3, is a protein which in humans is encoded by the LAG3 gene. (genscript.com)
• Our results demonstrated the feasibility of C12orf35 as the target site for exogenous gene integration, and strongly suggested that C12orf35 targeted integration mediated by CRISPR/Cas9 is a reliable strategy for the rapid development of recombinant CHO cell lines. (springer.com)
• Ding K, Han L, Zong H, Chen J, Zhang B, Zhu J (2017) Production process reproducibility and product quality consistency of transient gene expression in HEK293 cells with anti-PD1 antibody as the model protein. (springer.com)
• Point mutation in the Virbrio cholerae O139 cef (CHO cell elongating factor) gene alters the. (deepdyve.com)
• Shalu, O. 2012-02-20 00:00:00 Sequencing of the cef (CHO cell elongating factor) gene of Vibrio cholerae serogroup O139 revealed one nucleotide substitution (T to C at nucleotide 2015) as compared to cef of classical V. cholerae O1 and two substitutions (GT to AC at nucleotides 2014-2015) as compared to cef of V. cholerae O1 El Tor. (deepdyve.com)
• Through these studies, we have uncovered the dynamic range of secretion profiles of these analytes from single iPSC-derived neuronal and glial cells and have molecularly characterized subpopulations of these cells through immunostaining and gene expression analyses. (jneurosci.org)
• Fanconi anemia group G protein is a protein that in humans is encoded by the FANCG gene. (wikipedia.org)
• The present invention relates to human Tumor Necrosis Factor (TNF) binding Protein I, herein designated TBP-I, and more particularly, to the cloning of thee gene coding for said protein and its expression in host cells. (google.com)
• In our validation screen, we performed a drug-gene interaction analysis by exposing an edited pool of primary T cells to prolonged treatment with a sub-lethal dose of a specific small molecule inhibitor. (horizondiscovery.com)

Mammalian cell lines2

• Researchers from Technical University of Denmark have produced CHO or mammalian cell lines which can produce 1, 2 g/L recombinant Alpha-1-antitrypsin protein. (alpha1.uk)
• rhGH could be produced in mammalian cell lines at high levels with higher bioactivity. (bvsalud.org)

Genes8

• The human GABRA5, GABR2 and GABRG2 genes encode the Cl--selective, ionotropic GABAA receptor-channel. (creativebiomart.net)
• Furthermore, they removed a number of the CHO cells natural glycosylation genes using CRISPR-Cas9. (dtu.dk)
• CRISPR-Cas9 was used to remove a number of undesired natural glycosylation genes of CHO cells. (alpha1.uk)
• Although reporter genes have played a significant role in numerous applications, both in vitro and in vivo, they are most frequently used as indicators of transcriptional activity in cells . (bvsalud.org)
• Baser B, Spehr J, Bussow K, van den Heuvel J (2016) A method for specifically targeting two independent genomic integration sites for co-expression of genes in CHO cells. (springer.com)
• However, microarray studies also implicated additional transcriptional targets in GSI-I-dependent cell death, including genes in the unfolded protein response, nuclear factor-κB and p53 pathways. (pubmedcentralcanada.ca)
• hORFeome v3.1: A resource of human open reading frames representing over 10,000 human genes. (horizondiscovery.com)
• Our screen was able to identify novel genes that are essential for the survival of primary T cells and genes that modulate the response of the T cells to the small molecule inhibitor treatment. (horizondiscovery.com)

HEK2934

• HEK293 Human CD86 / B7-2 transfected / overexpressed for Western blot (WB) positive control. (bioon.com.cn)
• Mouse CD8A & CD8B Heterodimer HEK293 Cell Lysate (WB positive control) [ Ӧ? (bioon.com.cn)
• Human IL-23 (IL12B & IL23A Heterodimer) HEK293 Cell Lysate (WB positive control) [ Ӧ? (bioon.com.cn)
• HEK293 Human LILRB4 / CD85k / ILT3 transfected / overexpressed for Western blot (WB) positive control. (bioon.com.cn)

Antibody14

• SILu ™ Mab is a recombinant, stable isotope-labeled, human monoclonal antibody which incorporates [ 13 C 6 , 15 N 4 ]-Arginine and [ 13 C 6 , 15 N 2 ]-Lysine. (sigmaaldrich.com)
• SILu ™ MAB Stable-Isotope Labeled Universal Monoclonal Antibody Standard human has been used in a comparative study of automated antibody de novo sequencing. (sigmaaldrich.com)
• The λ light chain (C5λ) of human monoclonal antibody HB4C5 is expected to be potentially useful for lung cancer targeting. (elsevier.com)
• Cetuximab (anti-EGFR) is a recombinant chimeric monoclonal antibody that binds to the human epidermal growth factor receptor (EGFR) with high affinity. (selleckchem.com)
• The pharmacokinetics of hu1124, a human anti-CD11a antibody, were investigated in human subjects with psoriasis. (springer.com)
• Role of the LFA-1 molecule in cellular interactions required for antibody production in humans. (springer.com)
• TAKHZYRO is a first-of-its-kind fully human monoclonal antibody (mAb) that inhibits the activity of plasma kallikrein, an enzyme which is uncontrolled in people with HAE, to help prevent attacks. (yahoo.com)
• Trastuzumab is a humanized IgG1 monoclonal antibody that targets the HER2 receptor (human epidermal growth factor receptor 2 also known as HER2/neu) that is found on the cell membrane of epithelial cells. (invivogen.com)
• Binding of trastuzumab to HER2 results in cell death through different mechanisms including antibody-dependent cell-mediated cytotoxicity and phagocytosis [2, (invivogen.com)
• 2012. Trastuzumab induces antibody-dependent cell-mediated cytotoxicity (ADCC) in HER-2-non-amplified breast cancer cell lines. (invivogen.com)
• 2013. Trastuzumab mediates antibody-dependent cell- mediated cytotoxicity and phagocytosis to the same extent in both adjuvant and metastatic HER2/neu breast cancer patients. (invivogen.com)
• Ipilimumab is a fully human IgG1 monoclonal antibody that targets CTLA-4 (also known as CD152), a negative regulator of T cell activation. (invivogen.com)
• In addition, Ipilimumab induces antibody-dependent cell-mediated cytotoxicity (ADCC) and TNF-α production . (invivogen.com)
• 2013. The engagement of CTLA-4 on primary melanoma cell lines induces antibody-dependent cellular cytotoxicity and TNF-α production. (invivogen.com)

Species3

• Overall, the pharmacological characteristics of the human receptors are similar to other species with some species-specific characteristics. (biomedsearch.com)
• The main expression systems have members of the mammalian cell family, however, there are remarkable differences between the species. (kowsarpub.com)
• In the case of a subset of neurodegenerative disorders such as Alzheimer's disease (AD), there is growing evidence that neurotoxic species released from cells may underlie pathology. (jneurosci.org)

Adherent2

• The Quantum Cell Expansion System is a functionally closed and automated hollow-fiber bioreactor system that is designed to expand both adherent and suspension cells in a reproducible manner. (bioprocessintl.com)
• The hollow-fiber membrane requires a coating agent to help facilitate cellular adherence when culturing an adherent cell type such as MSCs. (bioprocessintl.com)

Membranes3

• Several experiments were performed to determine the optimum conditions for binding in FlashPlate coated with CHO cell membranes expressing the h5-HT 1B receptor. (bio-medicine.org)
• The aim of the present study was to better characterize effects of hydrogen peroxide, an important producer of oxygen free radicals, which diffuse easily through hydrophobic membranes ( Yu, 1994 ), on the gating properties of HERG expressed in a Chinese hamster ovary (CHO) cell line. (aspetjournals.org)
• In this study, the effects of activation of the human kappa receptor by agonists on [ 35 S]GTPγS binding to CHO cell membranes were examined. (aspetjournals.org)

Therapeutic5

• There is currently no treatment to safely desensitize mast cells to enable robust allergen immunotherapy with therapeutic levels of allergen. (jci.org)
• These data underscore the positive feedback regulation of endothelial tip cells by CCN1 through integrin alphavbeta3/VEGFR2 and increased YAP/TAZ activity, suggesting a promising therapeutic intervention for pathological angiogenesis. (nih.gov)
• By identifying which cells are responsible, we can better understand neural physiology and pathophysiology, more readily identify the underlying pathways responsible for analyte production, and ultimately use this information to guide the development of novel therapeutic strategies that target the cell types of relevance. (jneurosci.org)
• By binding CTLA-4, ipilimumab inhibits negative signals that physiologically downregulate T cell activation and exerts its therapeutic activity by upregulating the antitumor activity of T lymphocytes [1, (invivogen.com)
• The advent of CRISPR technologies and the use of T cells in personalized medicine have greatly accelerated both the therapeutic value of and interest in modified T cells. (horizondiscovery.com)

Produce Alpha-1-antitryps1

• A group of scientist from Novo Nordisk Foundation Center for Biosustainability at DTU has found a method to produce Alpha-1-antitrypsin protein in mammalian cells. (alpha1.uk)

20162

• Cheng JK, Lewis AM, Kim do S, Dyess T, Alper HS (2016) Identifying and retargeting transcriptional hot spots in the human genome. (springer.com)
• 2016) Cell Rep., 17(5):1438-1452. (horizondiscovery.com)

Endothelial cells5

• A high-affinity glycoprotein receptor of 170-235 kDa is expressed on vascular endothelial cells. (reprokine.com)
• Both endothelial cells and certain subsets of leukocytes express CD73. (hindawi.com)
• Endothelial (E)-selectin is a heavily glycosylated transmembrane protein expressed by activated endothelial cells in microvascular linings. (mpbio.com)
• LFA-1 interaction with ICAM-1 is one of at least three mechanisms for lymphocyte adhesion to cultured endothelial cells. (springer.com)
• Many of the local actions of snake venoms are mediated by a direct action on skeletal and vascular smooth muscle cells, endothelial cells, macrophages, neutrophils, mast cells, and platelets (17). (scielo.br)

Inhibits2

• Specifically, the authors show that delivery of liposomes containing both the specific antigen recognized by the mast cell-bound IgE and a high-affinity glycan ligand of the inhibitory receptor CD33 (CD33L) to targeted mast cells inhibits antigen-induced, FcεRI-dependent spleen tyrosine kinase (Syk) phosphorylation and downstream protein tyrosine kinase (PTK) phosphorylation, Ca++ flux, and β-hexosaminidase release (i.e., degranulation). (jci.org)
• Induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. (abcam.com)

Glycosylation2

• Normally, the CHO glycosylation profile of Alpha-1-antitrypsin is quite different from the human counterpart. (dtu.dk)
• Though human recombinant erythropoietin (rEPO) is produced in CHO cells , the loss in quality control is 80% due to incomplete glycosylation of the rEPO with low levels of fully glycosylated active rEPO. (bvsalud.org)

Erythropoietin4

• To investigate the current bottlenecks in cell-free glycoprotein production, we chose erythropoietin (40% glycosylated), an essential endogenous hormone which stimulates the development of red blood cells. (mdsoar.org)
• Here, we report the production of recombinant erythropoietin (EPO) using CHO cell-free system. (mdsoar.org)
• Erythropoietin is a glycohormone involved in the regulation of the blood cell levels. (bvsalud.org)
• The GPI anchor can be removed during the purification process to yield a therapeutically relevant recombinant erythropoietin molecule cells with a higher in vivo biological activity due to its high molecular weight of 40 kD. (bvsalud.org)

Jurkat2

• High Throughput Transfection of Stem Cells, Primary Cells and Difficult-to-Transfect Cell Lines: Jurkat, CHO, Human Skeletal Muscle Cells & Primary Neuronal Cell Transfection using a Scalable, Electroporation-Based Technology. (selectscience.net)
• Specifically, the transfection of Jurkat,CHO, human skeletal muscle cells and primary neuronal cells using MaxCyte's scalable electroporation technology and their performance in a variety of cell-based assays is described. (selectscience.net)

Reagents1

• This demonstrates how large-scale electroporation can be used to eliminate the reliance on stable cell lines and costly transfection reagents, by producing large numbers of quality transfected cells for use in high throughput cellular screening and profiling. (selectscience.net)

Characterization2

• These cells are valuable systems for further characterization of specific receptor subtypes and for the development of new ligands. (biomedsearch.com)
• The high-content interrogation of single cells with platforms optimized for the multiparameter characterization of cells in liquid and solid biopsy samples can. (nist.gov)

Isotype2

• Anti-HER2-Tra-hIgA2 features the constant region of the human IgA2 isotype and the variable region of trastuzumab. (invivogen.com)
• Anti-hCTLA4-hIgG4 (S228P) features the constant region of the human IgG4 (S228P) isotype and the variable region of ipilimumab. (invivogen.com)

Lines15

• 1. Remove the packaging cell lines from liquid nitrogen and carry out a quick thaw. (creativebiomart.net)
• In addition, intracellular accumulation of C5λ and upregulation of BiP was found in hyper-producing cell lines which were introduced E1A and ras oncogene. (elsevier.com)
• Site-specific integration of transgenes into CHO hot spots is an ideal strategy to overcome these challenges since it can generate isogenic cell lines with consistent productivity and stability. (springer.com)
• In this study, we investigated three sites with potential high transcriptional activities: C12orf35, HPRT, and GRIK1, to determine the possible transcriptional hot spots in CHO cells, and further construct a reliable site-specific integration strategy to develop recombinant cell lines efficiently. (springer.com)
• Stable cell lines were generated successfully after a single round of selection. (springer.com)
• Deep sequencing demonstrated that there was low level of off-target mutations caused by CRISPR/Cas9, but none of them contributed to the development process of transgene cell lines. (springer.com)
• Upstate has built a portfolio of ion channel cell lines to meet these new and evolving needs in the drug discovery industry. (bio-medicine.org)
• Our ion channel cell lines are available off of the shelf. (bio-medicine.org)
• These cell lines are fully validated using electrophysiology, stable (>25 passage) and licensed for drug discovery research. (bio-medicine.org)
• The objective is to provide the pharmaceutical industry with a range of ion channel cell lines to support drug discovery, lead optimisation and safety pharmacology functions. (bio-medicine.org)
• Effects of ROS on voltage-gated K + channels have been studied both in single cardiac myocytes isolated from animals and in transfected cell lines. (aspetjournals.org)
• As GSI-I (Z-LLNle-CHO) is also a derivative of a widely used proteosome inhibitor MG-132, we hypothesized that this compound might be active in precursor-B acute lymphoblastic leukemia (ALL) cell lines and patient samples. (pubmedcentralcanada.ca)
• Recent studies in multiple myeloma and in lymphoma-derived B-cell lines suggest that GSI sensitivity may be a general property of mature B-cell neoplasms. (pubmedcentralcanada.ca)
• Cetuximab is effective in K-ras wild-type, EGFR-expressing gastric cancer cell lines and xenografts. (selleckchem.com)
• Crown antibodies pass additional stringent quality requirements, including extended control sets, uniform results against multiple biologically relevant cell lines and tissues, and function in multiple applications. (abgent.com)

Chinese5

• Although few people realize it, modern medicine relies heavily on the ovarian cells of Chinese hamsters, not as a. (nist.gov)
• In this study, recombinant human growth hormone [rhGH] was produced in eukaryotic Chinese hamster ovary [CHO] cell and production and concentration of rhGH verified by ELISA and western blotting . (bvsalud.org)
• In the present study we expressed recombinant human interleukin-7 (rhIL-7) in Chinese hamster ovary ( CHO)-K1 cells. (rbmb.net)
• Chinese hamster ovary (CHO) cells are the most widely used mammalian hosts for recombinant protein production. (springer.com)
• PeproGrow AF-CHO is an animal component-free, serum-free, complete medium formulation for the in vitro cultivation of Chinese Hamster Ovary-S cells (Thermo Fisher Scientific catalog numbers 11619-012, R800-07, or A11557-01). (peprotech.com)

Adenosine receptor1

• In this study we present for the first time the comparative pharmacology of all known human adenosine receptor subtypes. (biomedsearch.com)

Specificity1

• Since the drug we treated the T cells with had a known mechanism of action, these hits directly supported the specificity, sensitivity and quality of our platform. (horizondiscovery.com)

20191

• Am J Respir Cell Mol Biol, 2019 Apr. (nih.gov)

Recombinant protein1

• 7. A method in accordance with claim 1 wherein said soluble recombinant protein having the tumor necrosis factor binding characteristics of human TBP-I consists of a protein extending from any one of residues 1-20 through any one of residues 180-182 of SEQ ID NO:2. (google.com)

Embryonic1

• Gibco™ Neurobasal™ Medium is a basal medium that meets the special cell culture requirements of pre-natal and embryonic neuronal cells when used with Gibco™ B-27™ Supplement. (fishersci.com)

Receptors6

• A member of the family of G-protein-coupled receptors, it functions at the surface of pigment producing cells (called melanocytes). (creative-biogene.com)
• Somatostatin receptors are activated by somatostatin secreted from nerve and endocrine cells. (innoget.com)
• The Somatostatin Receptors (SSTRs) are expressed in a tissue-specific manner and involved in the regulation of secretion of insulin, glucagon and growth hormone as well as cell growth induced by neuronal excitation in both the central and peripheral nervous systems. (innoget.com)
• Human 5-HT6 receptors are G protein-coupled receptors. (innoget.com)
• These immune cells are activated after recognition of pathogen nucleic acids by sensors such as Toll-like receptors (TLR). (nature.com)
• These receptors are expressed on the surfaces of a variety of different cells. (google.com)

Skeletal1

• Finally, infusion of amino acids in humans leads to the concomitant activation of S6K1, phosphorylation of IRS-1 Ser-1101, a reduction in IRS-1 function, and insulin resistance in skeletal muscle. (pnas.org)

Epithelial cells2

• As an example, in epithelial cells it was proposed that the movement of water could be directly linked to cystic fibrosis transmembrane conductance regulator (CFTR) protein activity through a cAMP-stimulated aqueous pore, or be dependent on aquaporin. (biologists.org)
• Integrins α v β 5 and α v β 6 Mediate IL-4-induced Collective Migration in Human Airway Epithelial Cells. (nih.gov)

Amino acids11

• The recombinant human CD86 consists of 467 amino acids and predicts a molecular mass of 53.1 kDa. (bioon.com.cn)
• VEGF is a homodimeric heavily glycosylated protein.The human factor occurs in several molecular variants of 121, 162, 145, 148, 165, 183, 189 , 206 amino acids, arising by alternative splicing of the mRNA.The splice forms of VEGF differ in biological properties such as the receptor types, which they recognize and their interaction with heparan sulfate proteoglycans. (reprokine.com)
• Amino acids influence the glucose uptake through GLUT4 in CHO-K1 cells under high glucose conditions. (biomedsearch.com)
• The present study was to explore whether amino acids as mixtures could enhance glucose uptake in CHO-K1 cells specifically. (biomedsearch.com)
• The cells in F-12K1 serum-free medium were exposed to normal (7 mM) and high glucose (12, 17 and 27 mM) in the presence and absence of amino acids mixture (AAM) in varying concentration (2.5, 5 and 10 mM). (biomedsearch.com)
• Addition of a mixture of amino acids was found to improve cell viability, which got altered by high glucose in the CHO-K1 cells. (biomedsearch.com)
• Vascular Endothelial Growth Factor Human Recombinant produced in CHO cells is a double, glycosylated, polypeptide chain containing 165 amino acids. (abcam.com)
• 4. The fusion protein of claim 1 , wherein the junction region comprises an IgG region wherein the LSLS amino acid sequence (amino acids 3-6 of SEQ ID NO:5) is mutated without generating a T-cell epitope. (google.ca)
• This complete medium contains L-alanyl-L-Glutamine, amino acids, vitamins, salts, and non-animal derived hydrolysates which is recommended for a 5 day batch culture with a seeding density of 0.5 x10 6 cells/mL. (peprotech.com)
• R-Spondin-1 Human Recombinant produced in CHO cells is a glycosylated monomer chain containing 233 amino acids and having a total molecular mass of 25.6kDa. (prospecbio.com)
• The recombinant human CCL7 consisting of 87 amino acids and has a calculated molecular mass of 10.5 kDa. (antikoerper-online.de)

Source1

• Pooled human cryoprecipitate (CPPT, Bonfils Blood Center) was examined as an alternative to FN because it is a rich source of extracellular matrix components, including fibrinogen (Freedman, 2010), and also because it was previously shown to help aid cellular adherence in tissue culture flasks (Nikolaychik, 1994). (bioprocessintl.com)

Neuronal3

• Used in the growth of neuronal cells from hippocampus, cortex and otregions of the brain. (fishersci.com)
• Further, we show that multiple cell types secrete high levels of Aβ and sAPPα, but cells expressing GABAergic neuronal markers are overrepresented. (jneurosci.org)
• Here, we have developed a methodology to examine secreted products relevant to AD from single cells and adapted this method to examine human neuronal and glial cells derived from induced pluripotent stem cells (iPSCs). (jneurosci.org)

Functional7

• The frozen cells approach to functional testing consists in the dissociation of cell culture from testing. (perkinelmer.com)
• These cells are then stored in liquid nitrogen or simply in a -80°C freezer, thawed and used directly in a functional, cellular GPCR test with previously validated performance. (perkinelmer.com)
• PerkinElmer's validated, ready-to-use AequoZen™or cAMPZen™ frozen, irradiated cells make it easier for you to perform functional testing of GPCRs. (perkinelmer.com)
• Our frozen, irradiated cAMPZen cells express a variety of GPCRs for binding and functional assays. (perkinelmer.co.uk)
• The human lymphocyte function-associated (HLFA) antigen and a related macrophage differentiation antigen (HMac-1): Functional effects of subunit-specific monoclonal antibodies. (springer.com)
• The TNFs affect cells In different ways, some of which resemble the functional modes of other inflammatory mediators, like interleukin-1 (IL-1) and interleukin-6 (IL-6). (google.com)
• Hultquist JF, Schumann K, Woo JM, Manganaro L, McGregor MJ, Doudna J, Simon V, Krogan NJ, Marson A. A Cas9 Ribonucleoprotein Platform for Functional Genetic Studies of HIV-Host Interactions in Primary Human T Cells . (horizondiscovery.com)

Cytoplasmic1

• The naturally occurring human BTLA monomer consists of a 127-amino-acid extracellular domain, a 21-amino-acid transmembrane domain, and a 111-amino-acid cytoplasmic domain. (peprotech.com)

Assays3

• Data in this poster is presented for the transient transfection of a variety of difficult-to-transfect cells and their use in downstream assays. (selectscience.net)
• Aequorin-based Ca2+ assays represent a new paradigm in drug discovery research for cell-based assays for Ca2+-coupled GPCRs and ion channels. (perkinelmer.co.uk)
• Enzymatic assays confirmed the ability of recombinant human PAP to hydrolyze [ 3 H]AMP, although at much lower rate than human CD73. (hindawi.com)

Sequence3

• A DNA sequence encoding the human CD86 (NP_008820.2) (Met1-His239) was expressed with the C-terminal His-tagged Fc region of human IgG1 at the C-terminus. (bioon.com.cn)
• A DNA sequence encoding the human LILRB4 (AAH26309.1) (Met1-Glu259) was expressed with a C-terminal polyhistidine tag. (bioon.com.cn)
• Protein Structure: A DNA sequence encoding the mature form of human CCL7 (NP_006264.2) (Gln 24-Leu 99) was expressed, with a polyhistide tag at the N-terminus. (antikoerper-online.de)

Affinity6

• The notion of xanthine-insensitivity of the A3 receptor should be dropped at least for the human receptor as xanthines with submicromolar affinity were found. (biomedsearch.com)
• Here, we demonstrated that liposomal nanoparticles bearing an allergen and a high-affinity glycan ligand of the inhibitory receptor CD33 profoundly suppressed IgE-mediated activation of mast cells, prevented anaphylaxis in Tg mice with mast cells expressing human CD33, and desensitized mice to subsequent allergen challenge for several days. (jci.org)
• In human thyroid slices, TSH activates these two intracellular cascades with a higher affinity for the adenylate cyclase activation (from 0.1 to 1 mU/ml TSH) than for phospholipase C activation (from 1 to 10 mU/ml TSH). (nih.gov)
• The rhAng4 secreted from rCHO cells was purified at a purification yield of 53.3% from the cultured medium using an anti-FLAG M2 agarose affinity gel. (kaist.ac.kr)
• The results demonstrated the production of a homogenous completely glycosylated human rEPO-g as a 42 kD band without any low molecular weight glycoform variants as shown by affinity chromatography followed by SDS-PAGE and anti- human EPO specific western blot . (bvsalud.org)
• It has been produced in CHO cells and purified by affinity chromatography with peptide M . (invivogen.com)

CRISPR6

• Before humans repurposed it, CRISPR/Cas9 was a sort of internal immune system bacteria use to defend themselves against phages, or viruses that infect bacteria, by slicing up the phages' DNA. (news-medical.net)
• The discovery of the first small-molecule inhibitors of the Streptococcus pyogenes Cas9 (SpCas9) protein could enable more precise control over CRISPR-Cas9-based genome editing, researchers report May 2 in the journal Cell. (news-medical.net)
• Bachu R, Bergareche I, Chasin LA (2015) CRISPR-Cas targeted plasmid integration into mammalian cells via non-homologous end joining. (springer.com)
• Horizon scientists have recently successfully completed a pooled lentiviral CRISPR-Cas9 knockout screen in primary T cells. (horizondiscovery.com)
• Our successful application of pooled CRISPR screens in primary T cells opens the doors to significant scientific discovery in crucial areas of research, including immuno-oncology. (horizondiscovery.com)
• 2018) Genome-wide CRISPR Screens in Primary Human T Cells Reveal Key Regulators of Immune Function . (horizondiscovery.com)

Dendritic cells3

• Plasmacytoid dendritic cells (pDC) are specialized in secretion of type I interferon in response to pathogens. (nature.com)
• Direct B-cell stimulation by peripheral blood monocyte-derived dendritic cells in idiopathic thrombo. (biomedsearch.com)
• Cetuximab promotes phagocytosis by dendritic cells . (selleckchem.com)

Measurement3

• Use of FlashPlate Technology for In Vitro Measurement of [35S]-GTPγS Binding in CHO Cells Expressing the Human 5-HT1B Receptor ( J. Watson and J.V. (bio-medicine.org)
• We have developed a method to assess the overall quality of a cell counting measurement. (nist.gov)
• Flexsafe STR® bags ensure excellent cell growth and robustness combined with single-use, non-invasive sensors for pH, DO, viable biomass monitoring and online glucose and lactate measurement. (sartorius.com)

Plasma containing Alpha-2

• In the US and other countries, the disorder is currently treated with fractions of human plasma containing Alpha-1-antitrypsin. (dtu.dk)
• The disorder is being treated in various countries including the United States by using human plasma containing Alpha-1-antitrypsin. (alpha1.uk)