One of the mechanisms by which CELL DEATH occurs (compare with NECROSIS and AUTOPHAGOCYTOSIS). Apoptosis is the mechanism responsible for the physiological deletion of cells and appears to be intrinsically programmed. It is characterized by distinctive morphologic changes in the nucleus and cytoplasm, chromatin cleavage at regularly spaced sites, and the endonucleolytic cleavage of genomic DNA; (DNA FRAGMENTATION); at internucleosomal sites. This mode of cell death serves as a balance to mitosis in regulating the size of animal tissues and in mediating pathologic processes associated with tumor growth.
A family of intracellular CYSTEINE ENDOPEPTIDASES that play a role in regulating INFLAMMATION and APOPTOSIS. They specifically cleave peptides at a CYSTEINE amino acid that follows an ASPARTIC ACID residue. Caspases are activated by proteolytic cleavage of a precursor form to yield large and small subunits that form the enzyme. Since the cleavage site within precursors matches the specificity of caspases, sequential activation of precursors by activated caspases can occur.
A short pro-domain caspase that plays an effector role in APOPTOSIS. It is activated by INITIATOR CASPASES such as CASPASE 9. Isoforms of this protein exist due to multiple alternative splicing of its MESSENGER RNA.
Splitting the DNA into shorter pieces by endonucleolytic DNA CLEAVAGE at multiple sites. It includes the internucleosomal DNA fragmentation, which along with chromatin condensation, are considered to be the hallmarks of APOPTOSIS.
An in situ method for detecting areas of DNA which are nicked during APOPTOSIS. Terminal deoxynucleotidyl transferase is used to add labeled dUTP, in a template-independent manner, to the 3 prime OH ends of either single- or double-stranded DNA. The terminal deoxynucleotidyl transferase nick end labeling, or TUNEL, assay labels apoptosis on a single-cell level, making it more sensitive than agarose gel electrophoresis for analysis of DNA FRAGMENTATION.
Membrane proteins encoded by the BCL-2 GENES and serving as potent inhibitors of cell death by APOPTOSIS. The proteins are found on mitochondrial, microsomal, and NUCLEAR MEMBRANE sites within many cell types. Overexpression of bcl-2 proteins, due to a translocation of the gene, is associated with follicular lymphoma.
A member of the Bcl-2 protein family and homologous partner of C-BCL-2 PROTO-ONCOGENE PROTEIN. It regulates the release of CYTOCHROME C and APOPTOSIS INDUCING FACTOR from the MITOCHONDRIA. Several isoforms of BCL2-associated X protein occur due to ALTERNATIVE SPLICING of the mRNA for this protein.
A tumor necrosis factor receptor subtype found in a variety of tissues and on activated LYMPHOCYTES. It has specificity for FAS LIGAND and plays a role in regulation of peripheral immune responses and APOPTOSIS. Multiple isoforms of the protein exist due to multiple ALTERNATIVE SPLICING. The activated receptor signals via a conserved death domain that associates with specific TNF RECEPTOR-ASSOCIATED FACTORS in the CYTOPLASM.
The engulfing and degradation of microorganisms; other cells that are dead, dying, or pathogenic; and foreign particles by phagocytic cells (PHAGOCYTES).
Endogenous and exogenous compounds and that either inhibit CASPASES or prevent their activation.
A long pro-domain caspase that contains a caspase recruitment domain in its pro-domain region. Caspase 9 is activated during cell stress by mitochondria-derived proapoptotic factors and by CARD SIGNALING ADAPTOR PROTEINS such as APOPTOTIC PROTEASE-ACTIVATING FACTOR 1. It activates APOPTOSIS by cleaving and activating EFFECTOR CASPASES.
A CARD signaling adaptor protein that plays a role in the mitochondria-stimulated apoptosis (APOPTOSIS, INTRINSIC PATHWAY). It binds to CYTOCHROME C in the CYTOSOL to form an APOPTOSOMAL PROTEIN COMPLEX and activates INITIATOR CASPASES such as CASPASE 9.
The span of viability of a cell characterized by the capacity to perform certain functions such as metabolism, growth, reproduction, some form of responsiveness, and adaptability.
Semiautonomous, self-reproducing organelles that occur in the cytoplasm of all cells of most, but not all, eukaryotes. Each mitochondrion is surrounded by a double limiting membrane. The inner membrane is highly invaginated, and its projections are called cristae. Mitochondria are the sites of the reactions of oxidative phosphorylation, which result in the formation of ATP. They contain distinctive RIBOSOMES, transfer RNAs (RNA, TRANSFER); AMINO ACYL T RNA SYNTHETASES; and elongation and termination factors. Mitochondria depend upon genes within the nucleus of the cells in which they reside for many essential messenger RNAs (RNA, MESSENGER). Mitochondria are believed to have arisen from aerobic bacteria that established a symbiotic relationship with primitive protoeukaryotes. (King & Stansfield, A Dictionary of Genetics, 4th ed)
A protein of the annexin family isolated from human PLACENTA and other tissues. It inhibits cytosolic PHOSPHOLIPASE A2, and displays anticoagulant activity.
A cell line derived from cultured tumor cells.
The pathological process occurring in cells that are dying from irreparable injuries. It is caused by the progressive, uncontrolled action of degradative ENZYMES, leading to MITOCHONDRIAL SWELLING, nuclear flocculation, and cell lysis. It is distinct it from APOPTOSIS, which is a normal, regulated cellular process.
A CELL LINE derived from human T-CELL LEUKEMIA and used to determine the mechanism of differential susceptibility to anti-cancer drugs and radiation.
A large group of proteins that control APOPTOSIS. This family of proteins includes many ONCOGENE PROTEINS as well as a wide variety of classes of INTRACELLULAR SIGNALING PEPTIDES AND PROTEINS such as CASPASES.
The intracellular transfer of information (biological activation/inhibition) through a signal pathway. In each signal transduction system, an activation/inhibition signal from a biologically active molecule (hormone, neurotransmitter) is mediated via the coupling of a receptor/enzyme to a second messenger system or to an ion channel. Signal transduction plays an important role in activating cellular functions, cell differentiation, and cell proliferation. Examples of signal transduction systems are the GAMMA-AMINOBUTYRIC ACID-postsynaptic receptor-calcium ion channel system, the receptor-mediated T-cell activation pathway, and the receptor-mediated activation of phospholipases. Those coupled to membrane depolarization or intracellular release of calcium include the receptor-mediated activation of cytotoxic functions in granulocytes and the synaptic potentiation of protein kinase activation. Some signal transduction pathways may be part of larger signal transduction pathways; for example, protein kinase activation is part of the platelet activation signal pathway.
Cytochromes of the c type that are found in eukaryotic MITOCHONDRIA. They serve as redox intermediates that accept electrons from MITOCHONDRIAL ELECTRON TRANSPORT COMPLEX III and transfer them to MITOCHONDRIAL ELECTRON TRANSPORT COMPLEX IV.
A long pro-domain caspase that contains a death effector domain in its pro-domain region. Caspase 8 plays a role in APOPTOSIS by cleaving and activating EFFECTOR CASPASES. Activation of this enzyme can occur via the interaction of its N-terminal death effector domain with DEATH DOMAIN RECEPTOR SIGNALING ADAPTOR PROTEINS.
Derivatives of phosphatidic acids in which the phosphoric acid is bound in ester linkage to a serine moiety. Complete hydrolysis yields 1 mole of glycerol, phosphoric acid and serine and 2 moles of fatty acids.
Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others.
The termination of the cell's ability to carry out vital functions such as metabolism, growth, reproduction, responsiveness, and adaptability.
A transmembrane protein belonging to the tumor necrosis factor superfamily that was originally discovered on cells of the lymphoid-myeloid lineage, including activated T-LYMPHOCYTES and NATURAL KILLER CELLS. It plays an important role in immune homeostasis and cell-mediated toxicity by binding to the FAS RECEPTOR and triggering APOPTOSIS.
Conversion of an inactive form of an enzyme to one possessing metabolic activity. It includes 1, activation by ions (activators); 2, activation by cofactors (coenzymes); and 3, conversion of an enzyme precursor (proenzyme or zymogen) to an active enzyme.
Nuclear phosphoprotein encoded by the p53 gene (GENES, P53) whose normal function is to control CELL PROLIFERATION and APOPTOSIS. A mutant or absent p53 protein has been found in LEUKEMIA; OSTEOSARCOMA; LUNG CANCER; and COLORECTAL CANCER.
A member of the bcl-2 protein family that plays a role in the regulation of APOPTOSIS. Two major isoforms of the protein exist due to ALTERNATIVE SPLICING of the BCL2L1 mRNA and are referred to as Bcl-XS and Bcl-XL.
Technique using an instrument system for making, processing, and displaying one or more measurements on individual cells obtained from a cell suspension. Cells are usually stained with one or more fluorescent dyes specific to cell components of interest, e.g., DNA, and fluorescence of each cell is measured as it rapidly transverses the excitation beam (laser or mercury arc lamp). Fluorescence provides a quantitative measure of various biochemical and biophysical properties of the cell, as well as a basis for cell sorting. Other measurable optical parameters include light absorption and light scattering, the latter being applicable to the measurement of cell size, shape, density, granularity, and stain uptake.
Cells grown in vitro from neoplastic tissue. If they can be established as a TUMOR CELL LINE, they can be propagated in cell culture indefinitely.
Exogenous and endogenous compounds which inhibit CYSTEINE ENDOPEPTIDASES.
Inhibitors of SERINE ENDOPEPTIDASES and sulfhydryl group-containing enzymes. They act as alkylating agents and are known to interfere in the translation process.
Established cell cultures that have the potential to propagate indefinitely.
Enzymes that catalyze the transfer of multiple ADP-RIBOSE groups from nicotinamide-adenine dinucleotide (NAD) onto protein targets, thus building up a linear or branched homopolymer of repeating ADP-ribose units i.e., POLY ADENOSINE DIPHOSPHATE RIBOSE.
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
Products of proto-oncogenes. Normally they do not have oncogenic or transforming properties, but are involved in the regulation or differentiation of cell growth. They often have protein kinase activity.
A short pro-domain caspase that plays an effector role in APOPTOSIS. It is activated by INITIATOR CASPASES such as CASPASE 3 and CASPASE 10. Several isoforms of this protein exist due to multiple alternative splicing of its MESSENGER RNA.
An indolocarbazole that is a potent PROTEIN KINASE C inhibitor which enhances cAMP-mediated responses in human neuroblastoma cells. (Biochem Biophys Res Commun 1995;214(3):1114-20)
The relatively long-lived phagocytic cell of mammalian tissues that are derived from blood MONOCYTES. Main types are PERITONEAL MACROPHAGES; ALVEOLAR MACROPHAGES; HISTIOCYTES; KUPFFER CELLS of the liver; and OSTEOCLASTS. They may further differentiate within chronic inflammatory lesions to EPITHELIOID CELLS or may fuse to form FOREIGN BODY GIANT CELLS or LANGHANS GIANT CELLS. (from The Dictionary of Cell Biology, Lackie and Dow, 3rd ed.)
A group of cytochromes with covalent thioether linkages between either or both of the vinyl side chains of protoheme and the protein. (Enzyme Nomenclature, 1992, p539)
The voltage difference, normally maintained at approximately -180mV, across the INNER MITOCHONDRIAL MEMBRANE, by a net movement of positive charge across the membrane. It is a major component of the PROTON MOTIVE FORCE in MITOCHONDRIA used to drive the synthesis of ATP.
A long pro-domain caspase that contains a caspase recruitment domain in its pro-domain region. Activation of this enzyme can occur via the interaction of its caspase recruitment domain with CARD SIGNALING ADAPTOR PROTEINS. Caspase 2 plays a role in APOPTOSIS by cleaving and activating effector pro-caspases. Several isoforms of this protein exist due to multiple alternative splicing of its MESSENGER RNA.
A short pro-domain caspase that plays an effector role in APOPTOSIS. It is activated by INITIATOR CASPASES such as CASPASE 7; CASPASE 8; and CASPASE 10. Isoforms of this protein exist due to multiple alternative splicing of its MESSENGER RNA.
All of the processes involved in increasing CELL NUMBER including CELL DIVISION.
Molecules or ions formed by the incomplete one-electron reduction of oxygen. These reactive oxygen intermediates include SINGLET OXYGEN; SUPEROXIDES; PEROXIDES; HYDROXYL RADICAL; and HYPOCHLOROUS ACID. They contribute to the microbicidal activity of PHAGOCYTES, regulation of signal transduction and gene expression, and the oxidative damage to NUCLEIC ACIDS; PROTEINS; and LIPIDS.
Compounds or agents that combine with an enzyme in such a manner as to prevent the normal substrate-enzyme combination and the catalytic reaction.
Substances that inhibit or prevent the proliferation of NEOPLASMS.
A multi-domain mitochondrial membrane protein and member of the bcl-2 Protein family. Bak protein interacts with TUMOR SUPPRESSOR PROTEIN P53 and promotes APOPTOSIS.
Elements of limited time intervals, contributing to particular results or situations.
A member of the Bcl-2 protein family that reversibly binds MEMBRANES. It is a pro-apoptotic protein that is activated by caspase cleavage.
A promyelocytic cell line derived from a patient with ACUTE PROMYELOCYTIC LEUKEMIA. HL-60 cells lack specific markers for LYMPHOID CELLS but express surface receptors for FC FRAGMENTS and COMPLEMENT SYSTEM PROTEINS. They also exhibit phagocytic activity and responsiveness to chemotactic stimuli. (From Hay et al., American Type Culture Collection, 7th ed, pp127-8)
The complex series of phenomena, occurring between the end of one CELL DIVISION and the end of the next, by which cellular material is duplicated and then divided between two daughter cells. The cell cycle includes INTERPHASE, which includes G0 PHASE; G1 PHASE; S PHASE; and G2 PHASE, and CELL DIVISION PHASE.
The B-cell leukemia/lymphoma-2 genes, responsible for blocking apoptosis in normal cells, and associated with follicular lymphoma when overexpressed. Overexpression results from the t(14;18) translocation. The human c-bcl-2 gene is located at 18q24 on the long arm of chromosome 18.
The fission of a CELL. It includes CYTOKINESIS, when the CYTOPLASM of a cell is divided, and CELL NUCLEUS DIVISION.
Histochemical localization of immunoreactive substances using labeled antibodies as reagents.
Injuries to DNA that introduce deviations from its normal, intact structure and which may, if left unrepaired, result in a MUTATION or a block of DNA REPLICATION. These deviations may be caused by physical or chemical agents and occur by natural or unnatural, introduced circumstances. They include the introduction of illegitimate bases during replication or by deamination or other modification of bases; the loss of a base from the DNA backbone leaving an abasic site; single-strand breaks; double strand breaks; and intrastrand (PYRIMIDINE DIMERS) or interstrand crosslinking. Damage can often be repaired (DNA REPAIR). If the damage is extensive, it can induce APOPTOSIS.
A conserved class of proteins that control APOPTOSIS in both VERTEBRATES and INVERTEBRATES. IAP proteins interact with and inhibit CASPASES, and they function as ANTI-APOPTOTIC PROTEINS. The protein class is defined by an approximately 80-amino acid motif called the baculoviral inhibitor of apoptosis repeat.
The relationship between the dose of an administered drug and the response of the organism to the drug.
Strains of mice in which certain GENES of their GENOMES have been disrupted, or "knocked-out". To produce knockouts, using RECOMBINANT DNA technology, the normal DNA sequence of the gene being studied is altered to prevent synthesis of a normal gene product. Cloned cells in which this DNA alteration is successful are then injected into mouse EMBRYOS to produce chimeric mice. The chimeric mice are then bred to yield a strain in which all the cells of the mouse contain the disrupted gene. Knockout mice are used as EXPERIMENTAL ANIMAL MODELS for diseases (DISEASE MODELS, ANIMAL) and to clarify the functions of the genes.
The uptake of naked or purified DNA by CELLS, usually meaning the process as it occurs in eukaryotic cells. It is analogous to bacterial transformation (TRANSFORMATION, BACTERIAL) and both are routinely employed in GENE TRANSFER TECHNIQUES.
Serum glycoprotein produced by activated MACROPHAGES and other mammalian MONONUCLEAR LEUKOCYTES. It has necrotizing activity against tumor cell lines and increases ability to reject tumor transplants. Also known as TNF-alpha, it is only 30% homologous to TNF-beta (LYMPHOTOXIN), but they share TNF RECEPTORS.
A flavoprotein that functions as a powerful antioxidant in the MITOCHONDRIA and promotes APOPTOSIS when released from the mitochondria. In mammalian cells AIF is released in response to pro-apoptotic protein members of the bcl-2 protein family. It translocates to the CELL NUCLEUS and binds DNA to stimulate CASPASE-independent CHROMATIN condensation.
The first continuously cultured human malignant CELL LINE, derived from the cervical carcinoma of Henrietta Lacks. These cells are used for VIRUS CULTIVATION and antitumor drug screening assays.
Agents obtained from higher plants that have demonstrable cytostatic or antineoplastic activity.
A transmembrane-protein belonging to the TNF family of intercellular signaling proteins. It is a widely expressed ligand that activates APOPTOSIS by binding to TNF-RELATED APOPTOSIS-INDUCING LIGAND RECEPTORS. The membrane-bound form of the protein can be cleaved by specific CYSTEINE ENDOPEPTIDASES to form a soluble ligand form.
A pro-apoptotic protein and member of the Bcl-2 protein family that is regulated by PHOSPHORYLATION. Unphosphorylated Bad protein inhibits the activity of BCL-XL PROTEIN.
A negative regulatory effect on physiological processes at the molecular, cellular, or systemic level. At the molecular level, the major regulatory sites include membrane receptors, genes (GENE EXPRESSION REGULATION), mRNAs (RNA, MESSENGER), and proteins.
Glycoproteins found on the membrane or surface of cells.
Within a eukaryotic cell, a membrane-limited body which contains chromosomes and one or more nucleoli (CELL NUCLEOLUS). The nuclear membrane consists of a double unit-type membrane which is perforated by a number of pores; the outermost membrane is continuous with the ENDOPLASMIC RETICULUM. A cell may contain more than one nucleus. (From Singleton & Sainsbury, Dictionary of Microbiology and Molecular Biology, 2d ed)
Quaternary ammonium analog of ethidium; an intercalating dye with a specific affinity to certain forms of DNA and, used as diiodide, to separate them in density gradients; also forms fluorescent complexes with cholinesterase which it inhibits.
RNA sequences that serve as templates for protein synthesis. Bacterial mRNAs are generally primary transcripts in that they do not require post-transcriptional processing. Eukaryotic mRNA is synthesized in the nucleus and must be exported to the cytoplasm for translation. Most eukaryotic mRNAs have a sequence of polyadenylic acid at the 3' end, referred to as the poly(A) tail. The function of this tail is not known for certain, but it may play a role in the export of mature mRNA from the nucleus as well as in helping stabilize some mRNA molecules by retarding their degradation in the cytoplasm.
Studies have found, that humans evolved on a diet with a ratio of omega-6 (n-6) to omega-3 (n-3) essential fatty acids (EFA) of ... It has pro-apoptotic activity. It operates independently of the known cannabinoid and vanilloid receptors targeted by ... In humans, plasma OEA levels are also found positively correlated with positive mood and emotions. OEA acting mostly at ... In addition to metabolism by FAAH, COX-2 and LOXs, NAE 20:4 (AEA) can also undergo oxidation by several of human cytochrome ...
Human BAG1 genome location and BAG1 gene details page in the UCSC Genome Browser. Tang SC (2003). "BAG-1, an anti-apoptotic ... It enhances the anti-apoptotic effects of BCL2 and represents a link between growth factor receptors and anti-apoptotic ... 1996). "Cloning of cDNAs encoding the human BAG1 protein and localization of the human BAG1 gene to chromosome 9p12". Genomics ... 1998). "p53-inducible human homologue of Drosophila seven in absentia (Siah) inhibits cell growth: suppression by BAG-1". EMBO ...
FMR1-AS1 has an anti-apoptotic function. Long noncoding RNA GRCh38: Ensembl release 89: ENSG00000268066 - Ensembl, May 2017 " ... "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine. Ladd PD, Smith LE, ...
Sterile alpha motif and leucine zipper containing kinase AZK, also known as ZAK, is a human gene. This gene is a member of the ... The protein also has pro-apoptotic activity. Alternate transcriptional splice variants, encoding different isoforms, have been ... 2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci ... 2004). "Complete sequencing and characterization of 21,243 full-length human cDNAs". Nat. Genet. 36 (1): 40-5. doi:10.1038/ ...
Human Hsc70 has 85% identity with human Hsp70 (SDSC workbench, blosom26 default analysis). The scientific community has long ... In later stages of apoptosis the entire cell becomes fragmented, forming a number of plasma membrane-bounded apoptotic bodies ... Sarkar S, Pollack BP, Lin KT, Kotenko SV, Cook JR, Lewis A, Pestka S (Dec 2001). "hTid-1, a human DnaJ protein, modulates the ... Ajuh P, Kuster B, Panov K, Zomerdijk JC, Mann M, Lamond AI (Dec 2000). "Functional analysis of the human CDC5L complex and ...
2006). "Clearance of apoptotic cells in human SLE". Apoptosis and Its Relevance to Autoimmunity. Curr. Dir. Autoimmun. Current ... SLE is associated with defects in apoptotic clearance, and the damaging effects caused by apoptotic debris. Early apoptotic ... Apoptotic cells also express "find-me" signals, to attract macrophages and dendritic cells. When apoptotic material is not ... In some people with SLE, buildup of apoptotic debris can be observed in GC because of an ineffective clearance of apoptotic ...
... is a protein that in humans is encoded by the DSG3 gene. In the skin epidermis Desmoglein-3 is expressed in the ... "The fate of desmosomal proteins in apoptotic cells". The Journal of Biological Chemistry. 276 (44): 41175-81. doi:10.1074/jbc. ... Silos SA, Tamai K, Li K, Kivirikko S, Kouba D, Christiano AM, Uitto J (July 1996). "Cloning of the gene for human pemphigus ... Wang Y, Amagai M, Minoshima S, Sakai K, Green KJ, Nishikawa T, Shimizu N (April 1994). "The human genes for desmogleins (DSG1 ...
The patriarch hypothesis is a hypothesis that explains the occurrence of menopause in human females and how a long post-fertile ... doi:10.1016/s0015-0282(00)00417-9. Hsueh, A. J. (1994). "Ovarian follicle atresia: A hormonally controlled apoptotic process". ... However, INK4a/ARF situated on the human chromosome 9p21 does appear to act as a tumour suppressor therefore extending ... Hudson, M. (2005). Why do females (especially human females) survive beyond an age at which they can reproduce? Sussex ...
Apoptotic chromatin condensation inducer in the nucleus is a protein that in humans is encoded by the ACIN1 gene. GRCh38: ... "Entrez Gene: ACIN1 apoptotic chromatin condensation inducer 1". Human ACIN1 genome location and ACIN1 gene details page in the ... 2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci ... Kim JE, Tannenbaum SR, White FM (2005). "Global phosphoproteome of HT-29 human colon adenocarcinoma cells". J. Proteome Res. 4 ...
Apoptotic protease activating factor 1, also known as APAF1, is a human homolog of C. elegans CED-4 gene. This gene encodes a ... Kim H, Jung YK, Kwon YK, Park SH (1999). "Assignment of apoptotic protease activating factor-1 gene (APAF1) to human chromosome ... Human APAF1 genome location and APAF1 gene details page in the UCSC Genome Browser. Overview of all the structural information ... "Entrez Gene: APAF1 apoptotic peptidase activating factor 1". CS1 maint: discouraged parameter (link) Zou H, Henzel WJ, Liu X, ...
... is its human gene and is pro-apoptotic. The protein encoded by this gene is a bZIP transcription factor which can bind as ... CEBPE protein, human at the US National Library of Medicine Medical Subject Headings (MeSH) Human CEBPE genome location and ... Chumakov AM, Grillier I, Chumakova E, Chih D, Slater J, Koeffler HP (Mar 1997). "Cloning of the novel human myeloid-cell- ... Antonson P, Stellan B, Yamanaka R, Xanthopoulos KG (Jul 1996). "A novel human CCAAT/enhancer binding protein gene, C/EBPepsilon ...
We are far, but if we come to better understand the steps of this phenomenon in the Human, a coroner could via a "postmortem ... Javan, G. T., Can, I., Finley, S. J., & Soni, S. (2015). The apoptotic thanatotranscriptome associated with the liver of ... Whether there is the same in humans, because previous studies have already shown that in people dead by trauma, heart attack or ... Some authors in 2015 introduced the concept of "thanatotranscriptome apoptotic" Test another hypothesis is that after death, a ...
Their apoptotic cells are similar to homologous to mammalian. However, maintenance of long-lived cells involves proteins such ... as SDLAGL that are highly similar to yeast and human homologs. Certain inorganic materials, such as iron and selenium, ... Researchers have discovered that apoptotic factors are induced in the tissue that is rejected. Suberites consist of many ... Suberites can be susceptible to infection which often stimulates cell death through apoptotic pathways. Suberites, namely S. ...
... human gene location in the UCSC Genome Browser. SAP18 human gene details in the UCSC Genome Browser. v t e. ... Additionally, SAP18s splice variants are implicated in apoptotic cycles. SAP18 has been shown to interact with POLE2, and RGS10 ... Histone deacetylase complex subunit SAP18 is an enzyme that in humans is encoded by the SAP18 gene. Histone acetylation plays a ... GRCh38: Ensembl release 89: ENSG00000150459 - Ensembl, May 2017 "Human PubMed Reference:". National Center for Biotechnology ...
In human cardiac tissue, this gene was found to affect cell growth and death while in neural tissue it affected neuronal growth ... December 2003). "IRAS is an anti-apoptotic protein". Annals of the New York Academy of Sciences. 1009 (1): 400-12. Bibcode: ... In humans, this protein has been shown to bind to the adapter insulin receptor substrate 4 (IRS4) to mediate translocation of ... Nischarin is a protein that in humans is encoded by the NISCH gene. This gene encodes a nonadrenergic imidazoline-1 receptor ...
Suo, Huayi; Sun, Peng; Wang, Cun; Peng, Deguang; Zhao, Xin (2016). "Apoptotic effects of insect tea in HepG2 human hepatoma ... and claimed effects on human health. Insect teas are widely used in traditional Asian medicine, particularly in China. A few ...
Eyes absent homolog 2 is a protein that in humans is encoded by the EYA2 gene. This gene encodes a member of the eyes absent ( ... Clark SW, Fee BE, Cleveland JL (Feb 2002). "Misexpression of the eyes absent family triggers the apoptotic program". The ... PDBe-KB provides an overview of all the structure information available in the PDB for Human Eyes absent homolog 2 (EYA2) v t e ... Fee BE, Doyle CA, Cleveland JL (Feb 2002). "A novel Eyes Absent 2 protein is expressed in the human eye". Gene. 285 (1-2): 221- ...
Apoptosis regulator BAX, also known as bcl-2-like protein 4, is a protein that in humans is encoded by the BAX gene. BAX is a ... The BAX gene was the first identified pro-apoptotic member of the Bcl-2 protein family. Bcl-2 family members share one or more ... Human BAX genome location and BAX gene details page in the UCSC Genome Browser. Overview of all the structural information ... Komatsu K, Miyashita T, Hang H, Hopkins KM, Zheng W, Cuddeback S, Yamada M, Lieberman HB, Wang HG (2000). "Human homologue of S ...
Macrophages have also been found actively moving in and out of the vacuoles inside the TNCs during the times of high apoptotic ... "The human thymus microenvironment: in vivo identification of thymic nurse cells and other antigenically-distinct subpopulations ... "The human thymus microenvironment: in vivo identification of thymic nurse cells and other antigenically-distinct subpopulations ... Thus, since not all thymocytes internalized by TNCs went through apoptotic pathways, this was used to conclude that thymic ...
Apoptotic mechanisms in the CNS are still not well understood, yet it is thought that apoptosis of interneurons is a self- ... In humans, PCD in progenitor cells starts at gestational week 7 and remains until the first trimester. This process of cell ... The BCR-ABL oncogene has been found to be involved in the development of cancer in humans. c-Myc is involved in the regulation ... Several pro-apoptotic signals, such as TNF, TRAIL, and FADD, also induce autophagy. Additionally, Bcl-2 inhibits Beclin-1- ...
Sanchez-Margalet V, Martin-Romero C (July 2001). "Human leptin signaling in human peripheral blood mononuclear cells: ... It has anti-apoptotic as well as proliferative effects. STAT3 can promote oncogenesis by being constitutively active through ... In the report on human glioblastoma tumor, or brain cancer, STAT3 was shown to have an oncogenic or a tumor suppressor role ... Signal transducer and activator of transcription 3 (STAT3) is a transcription factor which in humans is encoded by the STAT3 ...
DnaJ homolog subfamily C member 3 is a protein that in humans is encoded by the DNAJC3 gene. The protein encoded by this gene ... In later stages of apoptosis the entire cell becomes fragmented, forming a number of plasma membrane-bounded apoptotic bodies ... Ladiges W, Morton J, Hopkins H, Wilson R, Filley G, Ware C, Gale M (Mar 2002). "Expression of human PKR protein kinase in ... Korth MJ, Edelhoff S, Disteche CM, Katze MG (Jan 1996). "Chromosomal assignment of the gene encoding the human 58-kDa inhibitor ...
FOXO1A+protein,+human at the US National Library of Medicine Medical Subject Headings (MeSH) This article incorporates text ... FOXO1 upregulates Fas ligand (FasL) transcriptionally resulting in apoptotic cell death. Additionally, FOXO1 trans-activate Bim ... Forkhead box protein O1 (FOXO1), also known as forkhead in rhabdomyosarcoma (FKHR), is a protein that in humans is encoded by ... Further, it was revealed that DNA damage-induced cell death in p53-deficient and p53-proficient cells was reduced when human ...
... and functional analysis of a novel human cDNA (BNIP3L) encoding a protein homologous to human NIP3". Genes Chromosomes Cancer. ... also an apoptotic protector. The protein encoded by this gene is a functional homolog of BNIP3, a proapoptotic protein. This ... "Toward a catalog of human genes and proteins: sequencing and analysis of 500 novel complete protein coding human cDNAs". Genome ... Human BNIP3L genome location and BNIP3L gene details page in the UCSC Genome Browser. Chen G, Cizeau J, Vande Velde C, Park JH ...
Neural development Axon guidance Pioneer neuron Pioneer axon Neural development in humans Human brain development timeline ... The absence of netrin 1 is responsible for apoptosis, while the presence of netrin 1 leads to an inhibition of the apoptotic ... In various human cancers, it has been shown that netrin becomes over-expressed. It has also been shown that certain receptors ... This has been observed in the human colon epithelium, where higher levels of natural cell death at the upper portion of the ...
Detection of viral DNA is also viable as the nucleosomal DNA of virus is subjected to apoptotic fragmentation. Cell-free fetal ... DNA fragments of the human immunodeficiency virus (HIV), Plasmodium, and Leishmania can be detected in the infected individuals ... As a result, urine samples contain cfDNA derived from apoptotic tumor cells, making ucfDNA favorable for the diagnosis of ... This is due to the apoptotic cells originating from the pathogens. Pathogen detection in urine covers for parasites, viruses, ...
Human BNIP3 genome location and BNIP3 gene details page in the UCSC Genome Browser. As of this edit, this article uses content ... BNIP3 is a member of the apoptotic Bcl-2 protein family. It can induce cell death while also assisting with cell survival. Like ... July 2003). "BNIP3 plays a role in hypoxic cell death in human epithelial cells that is inhibited by growth factors EGF and IGF ... BCL2/adenovirus E1B 19 kDa protein-interacting protein 3 is a protein that in humans is encoded by the BNIP3 gene. ...
Campanella ME, Chu H, Low PS (February 2005). "Assembly and regulation of a glycolytic enzyme complex on the human erythrocyte ... In addition, nuclear translocation of GAPDH has been reported in Parkinson's disease (PD), and several anti-apoptotic PD drugs ... GAPDH is overexpressed in multiple human cancers, such as cutaneous melanoma, and its expression is positively correlated with ... strongly reduces the apoptotic action of GAPDH by preventing its S-nitrosylation and might thus be used as a drug. GAPDH acts ...
But there was no evidence that p53 played a major role in human cancers, and the gene encoding p53 (TP53) was thought to be an ... Yu J, Wang Z, Kinzler KW, Vogelstein B, Zhang L (February 2003). "PUMA mediates the apoptotic response to p53 in colorectal ... In 1989, Vogelstein and his students discovered that TP53 not only played a role in human tumorigenesis, but that it was a ... Waldman T, Kinzler KW, Vogelstein B (1995). "p21 is necessary for the p53-mediated G1 arrest in human cancer cells". Cancer Res ...
Pathogen-infected apoptotic cells will trigger inflammation, but damaged cells that are degraded as part of the normal tissue ... which causes Legionnaire's disease in humans. Phagosome maturation in amoeba is very similar to that in macrophages, so they ... It is only in the adult that apoptotic cells are phagocytosed by professional phagocytes. Inflammation is only triggered by ... Phagosomes degrade senescent cells and apoptotic cells to maintain tissue homeostasis. Erythrocytes have one of the highest ...
regulation of apoptotic process. · cellular protein metabolic process. · ventricular cardiac muscle cell development. · ... Human laminopathies: nuclei gone genetically awry. Nat. Rev. Genet. December 2006, 7 (12): 940-52. PMID 17139325. doi:10.1038/ ... apoptotic process. · cellular component disassembly involved in execution phase of apoptosis. · activation of signaling protein ... Life at the edge: the nuclear envelope and human disease. Nat. Rev. Mol. Cell Biol. 2002, 3 (8): 575-85. PMID 12154369. doi: ...
Paired box gene 8, also known as PAX8, is a protein which in humans is encoded by the PAX8 gene.[5] ... negative regulation of apoptotic process involved in metanephric nephron tubule development. • S-shaped body morphogenesis. • ... regulation of apoptotic process. • pronephros development. • regulation of metanephric nephron tubule epithelial cell ... negative regulation of apoptotic process involved in metanephric collecting duct development. • kidney development. • ...
The disease is caused by yellow fever virus and is spread by the bite of an infected female mosquito.[3] It infects only humans ... Apoptotic masses known as Councilman bodies appear in the cytoplasm of hepatocytes.[29][30] ... Barnes, E. (2005). Diseases and Human Evolution. Albuquerque: University of New Mexico. ISBN 978-0-8263-3065-9. .. ... Pyriproxyfen is recommended as a chemical larvicide, mainly because it is safe for humans and effective even in small doses.[4] ...
Presenilin-1 (PS-1) is a presenilin protein that in humans is encoded by the PSEN1 gene.[5] Presenilin-1 is one of the four ... apoptotic process. • activation of MAPKK activity. • thymus development. • positive regulation of coagulation. • negative ... neuron apoptotic process. • single organismal cell-cell adhesion. • smooth endoplasmic reticulum calcium ion homeostasis. • ... negative regulation of apoptotic process. • negative regulation of transcription from RNA polymerase II promoter. • proteolysis ...
... of the human genome in five human cell lines". Genome Research. 17 (6): 691-707. doi:10.1101/gr.5704207. PMC 1891331. PMID ... "Apoptotic phosphorylation of histone H2B is mediated by mammalian sterile twenty kinase". Cell. 113 (4): 507-17. doi:10.1016/ ... a length to width ratio of more than 10 million to 1 in human DNA). For example, each human diploid cell (containing 23 pairs ... Youngson RM (2006). Collins Dictionary of Human Biology. Glasgow: HarperCollins. ISBN 978-0-00-722134-9. .. .mw-parser-output ...
Frizzled-5 is a protein that in humans is encoded by the FZD5 gene.[5][6][7] ... apoptotic process involved in morphogenesis. • regulation of chorionic trophoblast cell proliferation. • regulation of ... 2005). "Generation and annotation of the DNA sequences of human chromosomes 2 and 4". Nature. 434 (7034): 724-31. doi:10.1038/ ... Saitoh T, Hirai M, Katoh M (Jun 2001). "Molecular cloning and characterization of human Frizzled-5 gene on chromosome 2q33.3- ...
Human PubMed Reference:». *↑ Schneider F; et al. (2009). «NPM1 but not FLT3-ITD mutations predict early blast cell clearance ... negative regulation of apoptotic process. • regulation of DNA damage response, signal transduction by p53 class mediator. • ... 1995). «Construction of a human full-length cDNA bank.». Gene. 150 (2): 243-50. PMID 7821789. doi:10.1016/0378-1119(94)90433-2 ... Hale TK, Mansfield BC (1990). «Nucleotide sequence of a cDNA clone representing a third allele of human protein B23.». Nucleic ...
In vitro, exposure of human bronchial epithelial cells or human pulmonary alveolar epithelial cells to agents such as hydrogen ... inflammatory response characterized by massive infiltration of F4/80+ monocytic/macrophage-like cells and a wave of apoptotic ... As a highly toxic and irritating material, it is hazardous to human health, and was involved in the Bhopal disaster-which ... 2011) Smad molecules expression pattern in human bronchial airway induced by sulfur mustard. Iran J Allergy Asthma Immunol. 10: ...
"FDA approves human embryonic stem cell study". 2009년 1월 23일.. ... "DNA repair/pro-apoptotic dual-role proteins in five major DNA repair pathways: fail-safe protection against carcinogenesis" ... Thomson, James A.; Zwaka (2003년 2월 10일). "Homologous recombination in human embryonic stem cells". 》Nature Biotechnology》 21 (3 ... "Blastocysts Embryonic Stem Cell Lines Derived from Human". 》Science》 282 (5391): 1145-1147. PMID 9804556. doi:10.1126/science. ...
... (BDNF), or abrineurin,[5] is a protein[6] that, in humans, is encoded by the BDNF gene.[7][8] ... negative regulation of neuron apoptotic process. • synapse assembly. • cell-cell signaling. • positive regulation of brain- ... "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.. .mw-parser-output ... The BDNF protein is encoded by a gene that is also called BDNF, found in humans on chromosome 11.[7][8] Structurally, BDNF ...
"TRAIL contributes to the apoptotic effect of 13-cis retinoic acid in human sebaceous gland cells". The British Journal of ... Dry eyes are very common during treatment and is caused by isotretinoin's apoptotic effect on the meibomian glands. Some people ... Three metabolites of Isotretinoin are detectable in human plasma after oral administration: 4-oxo-isotretinoin, retinoid acid ( ... "13-cis Retinoic acid induces apoptosis and cell cycle arrest in human SEB-1 sebocytes". The Journal of Investigative ...
Both mouse and human in vivo models of HER2-overexpressing breast cancers relied critically on this HER2-IL-6-STAT3 signaling ... one group showed that STAT3 and RANTES contribute to the maintenance of drug resistance by upregulating anti-apoptotic signals ... Furthermore, in human breast cancer, interference with the de-regulated Wnt signaling pathway reduces proliferation and ... It has been shown that phosphorylation of STAT3 and RANTES expression are increased in response to tamoxifen in human breast ...
Transplantation of stem cells is also known to cause toxicity and graft-versus-host disease (GVHD). Apoptotic cells have been ... "Human herpesvirus-6 entry into the central nervous system through the olfactory pathway". Proceedings of the National Academy ... Pessach I, Shimoni A, Nagler A (November 2012). "Apoptotic cells in allogeneic hematopoietic stem cell transplantations: " ...
negative regulation of apoptotic process. • positive regulation of protein kinase C activity. • positive regulation of NIK/NF- ... Yang ZQ، Moffa AB، Haddad R، Streicher KL، Ethier SP (September 2007). "Transforming properties of TC-1 in human breast cancer ... "Multiple interacting oncogenes on the 8p11-p12 amplicon in human breast cancer". Cancer Research. 66 (24): 11632-43. PMID ...
"Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.. ... negative regulation of apoptotic process. • osteoblast development. • innate immune response. • neutrophil degranulation. ... Human CLEC5A genome location and CLEC5A gene details page in the UCSC Genome Browser. ... negative regulation of myeloid cell apoptotic process. • GO:0022415 viral process. • signal transduction. • immune system ...
A number of infectious diseases can sometimes cause ALS-like symptoms,[4] including human immunodeficiency virus (HIV), human T ... anti-apoptotic drugs, and drugs to improve mitochondria function.[138] ... meaning that multiple copies of the mutant human gene are inserted into the transgenic model, and partly because the human ... These trials in humans went ahead on the basis of positive results in SOD1 transgenic mice, which are not a good animal model ...
Due to its role in generating the activated form of NF-κB, an anti-apoptotic and pro-inflammatory regulator of cytokine ... Yet another type of non-ATPase regulatory particle is the Blm10 (yeast) or PA200/PSME4 (human). It opens only one α subunit in ... Gankyrin is anti-apoptotic and has been shown to be overexpressed in some tumor cell types such as hepatocellular carcinoma.[81 ... Huang X, Luan B, Wu J, Shi Y (September 2016). "An atomic structure of the human 26S proteasome". Nature Structural & Molecular ...
Milton NG (October 2002). "Anandamide and noladin ether prevent neurotoxicity of the human amyloid-beta peptide". Neurosci. ... in the APP and BACE1 promoter sequences and implications in activating apoptotic genes and in amyloidogenesis". Gene 488 (1-2 ... first-in-human study". Lancet Neurol 11 (7): 597-604. PMID 22677258. doi:10.1016/S1474-4422(12)70140-0. Resumo divulgativo - ... "Days-to-criterion as an indicator of toxicity associated with human Alzheimer amyloid-β oligomers". Annals of Neurology 67 (6 ...
positive regulation of apoptotic process. • tumor necrosis factor-mediated signaling pathway. • axon guidance. • negative ... Fonatsch C, Latza U, Dürkop H, Rieder H, Stein H (Nov 1992). "Assignment of the human CD30 (Ki-1) gene to 1p36". Genomics. 14 ( ... apoptotic signaling pathway. • cellular response to mechanical stimulus. • response to lipopolysaccharide. • inflammatory ... Human TNFRSF8 genome location and TNFRSF8 gene details page in the UCSC Genome Browser. ...
A high rate above the survival threshold may kill cancer cells with a high apoptotic threshold.[86][87] This technique can be ... Proteins involved in autophagy are reduced with age in both human and mouse articular cartilage.[76] Mechanical injury to ... Knowledge of ATG genes provided scientists more convenient tools to dissect functions of autophagy in human health and disease ... or that degrade apoptotic mediators through autophagy: in such cases, use of inhibitors of the late stages of autophagy (such ...
... also known as cell division protein kinase 4 is an enzyme that in humans is encoded by the CDK4 gene ... positive regulation of apoptotic process. • regulation of gene expression. • cell cycle. • positive regulation of G2/M ... 1993). "Coamplification of the CDK4 gene with MDM2 and GLI in human sarcomas". Cancer Res. 53 (22): 5535-41. PMID 8221695.. ... Ghavidel A, Cagney G, Emili A (2005). "A skeleton of the human protein interactome". Cell. 122 (6): 830-2. doi:10.1016/j.cell. ...
extrinsic apoptotic signaling pathway. • extrinsic apoptotic signaling pathway via death domain receptors. • negative ... The human TNF gene (TNFA) was cloned in 1985.[22] It maps to chromosome 6p21.3, spans about 3 kilobases and contains 4 exons. ... apoptotic signaling pathway. • negative regulation of alkaline phosphatase activity. • regulation of I-kappaB kinase/NF-kappaB ... Dysregulation of TNF production has been implicated in a variety of human diseases including Alzheimer's disease,[6] cancer,[7] ...
It is known that lamotrigine is a weak inhibitor of human dihydrofolate reductase (DHFR) and other, more powerful, human DHFR ... Lamotrigine has been implicated in the apoptotic neurodegeneration of the developing brain.[52] GlaxoSmithKline investigated ... Shiah IS, Yatham LN, Gau YC, Baker GB (May 2003). "Effect of lamotrigine on plasma GABA levels in healthy humans". Progress in ... "Safety Alerts for Human Medical Products - Lamictal (lamotrigine): Drug Safety Communication - Serious Immune System Reaction" ...
Release of pro-apoptotic factors was also abrogated as well as a general increase in histone acetylation.[54] A human trial ... European Journal of Human Genetics : EJHG. 21 (6): 643-52. doi:10.1038/ejhg.2012.222. PMC 3658191. PMID 23073311.. ... American Journal of Human Genetics. 85 (3): 408-13. doi:10.1016/j.ajhg.2009.08.002. PMC 2771537. PMID 19716110.. ... some experiments have been performed on human cells as well as in human drug trials (see table below). There are inherent risks ...
... thought to be due to human ingestion of diseased individuals, and vCJD, thought to be due to human ingestion of BSE-tainted ... negative regulation of apoptotic process. • response to oxidative stress. • negative regulation of sequence-specific DNA ... "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.. .mw-parser-output ... The human protein structure consists of a globular domain with three α-helices and a two-strand antiparallel β-sheet, an NH2- ...
G-protein coupled receptor 120 is a protein that in humans is encoded by the GPR120 gene.[5][6] ... negative regulation of apoptotic process. • response to peptide. • cellular response to hormone stimulus. • negative regulation ... 2012). "Dysfunction of lipid sensor GPR120 leads to obesity in both mouse and human". Nature. 483 (7389): 350-4. doi:10.1038/ ... Fredriksson R, Höglund PJ, Gloriam DE, Lagerström MC, Schiöth HB (Nov 2003). "Seven evolutionarily conserved human rhodopsin G ...
Graham A, Heath P, Morten JE, Markham AF (March 1991). "The human aldose reductase gene maps to chromosome region 7q35". Human ... negative regulation of apoptotic process. • renal system development. • carbohydrate metabolic process. • electron transport ... Graham A, Heath P, Morten JE, Markham AF (March 1991). "The human aldose reductase gene maps to chromosome region 7q35". Human ... Aldo-keto reductase family 1, member B1 (AKR1B1), also known as aldose reductase, is an enzyme that in humans is encoded by the ...
... , also known as BDNF, is a protein[5] that, in humans, is encoded by the BDNF gene.[6][7] BDNF ... negative regulation of neuron apoptotic process. • synapse assembly. • cell-cell signaling. • positive regulation of brain- ... The BDNF protein is encoded by a gene that is also called BDNF, found in humans on chromosome 11.[6][7] Structurally, BDNF ... Human BDNF genome location and BDNF gene details page in the UCSC Genome Browser. ...
The human body has many defense mechanisms against pathogens, one of which is humoral immunity. This defence mechanism produces ... The immune response to RNP may be caused by the presentation of the nuclear components on the cell membrane in apoptotic blebs ... A secondary anti-human antibody conjugated to an enzyme such as horseradish peroxidase is added. The enzyme reaction will ... These can be visualised by adding a fluorescent tagged (usually FITC or rhodopsin B) anti-human antibody that binds to the ...
"Promoter Methylation Pattern Controls Corticotropin Releasing Hormone Gene Activity in Human Trophoblasts. ". PLoS One. 2017. ... hormone-mediated apoptotic signaling pathway. • positive regulation of insulin secretion involved in cellular response to ... "Corticotropin-releasing factor (CRF) system localization in human fetal heart. ". Hormones (Athens). 2016. PMID 27377597. ... "Human PubMed Reference:". https://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&cmd=Link&LinkName=gene_pubmed&from_uid=1392. ...
Shahneh FZ, Baradaran B, Orangi M, Zamani F. In vitro cytotoxic and apoptotic activity of four Persian medicine plants on human ... Apoptotic effects of non-edible parts of Punica granatum on human multiple myeloma cells. ... These results documented the cytotoxic and apoptotic effects of P. granatum extracts on human U266 multiple myeloma cells via ... Anticancer therapy targeting the apoptotic pathway. Lancet Oncol. 2003;4:721-9.CrossRefPubMedGoogle Scholar ...
In the current study, human monoclonal antibodies with binding specificity for apoptotic cells were isolated from the bone ... This may suggest a potentially great heterogeneity in human antibodies recognizing PC-related epitopes on apoptotic cells. To ... Interestingly, three of the four identified apoptotic cell-specific antibody clones were encoded by VH3 region rearrangements ... Our findings may have implication for improved prognostic testing and therapeutic interventions in human inflammatory disease. ...
miR-526a regulates apoptotic cell growth in human carcinoma cells.. [Xiaoli Yang, Cui Wang, Changzhi Xu, Zhifeng Yan, Congwen ...
Wright stain of human macrophages after exposure to either murine apoptotic thymocytes (C and D) or autologous human apoptotic ... A) Apoptotic murine thymocytes were offered to human macrophages in the presence of 15% normal non- heat-inactivated human ... 1994) Activation of the alternative pathway of human complement by apoptotic human umbilical vein endothelial cells. J Biochem ... 1998) Human CD14 mediates recognition and phagocytosis of apoptotic cells. Nature 392:505-509, pmid:9548256.. ...
Anti-apoptotic members of the Bcl-2 family, including … ... Mcl-1 is an anti-apoptotic factor for human hepatocellular ... Anti-apoptotic members of the Bcl-2 family, including myeloid cell leukemia-1 (Mcl-1), which regulate intrinsic apoptosis ... In this study, we analyzed the relevance of Mcl-1 expression for the apop-tosis resistance of human HCC. Mcl-1 protein ... Our data suggest that Mcl-1 is an important factor for the apoptosis resistance of human HCC, and constitutes an interesting ...
HIV-1 Infection of Human MDMs Does Not Activate Apoptotic Effector Caspases or Lead to Cell Death.. To understand the dynamics ... Inhibition of the lncRNA SAF drives activation of apoptotic effector caspases in HIV-1-infected human macrophages. Saikat ... Inhibition of the lncRNA SAF drives activation of apoptotic effector caspases in HIV-1-infected human macrophages ... Inhibition of the lncRNA SAF drives activation of apoptotic effector caspases in HIV-1-infected human macrophages ...
Human cells consistently displayed significantly reduced apoptotic function relative to the chimpanzee and macaque cells. These ... In this study, we sought to further test the hypothesis that humans maintain a reduced apoptotic function relative to ... chimpanzees by conducting a series of apoptotic function assays on human, chimpanzee and macaque primary fibroblastic cells. ... results are consistent with earlier findings indicating that apoptotic function is reduced in humans relative to chimpanzees. ...
... *Authors: *Kamran Harati ... The first significant apoptotic response was observed after 6 h of incubation with 20.1±2.9% of the cells left apoptotic and ... apoptotic effects of pycnogenol on HT1080 human fibrosarcoma cells. International Journal of Oncology 46, no. 4 (2015): 1629- ... apoptotic effects of pycnogenol on HT1080 human fibrosarcoma cells. International Journal of Oncology 46.4 (2015): 1629-1636. ...
Exposure of human leukemic cell lines (HL-60, ML-1, U-937, MOLT-4, EOL-1) to short direct-current (d.c.) treatment induced ... Induction of apoptotic cell death by direct-current treatment in human leukemic cell lines. *Masatsugu Kurokawa. 1. , ... Kurokawa, M., Sakagami, H., Kokubu, F. et al. Induction of apoptotic cell death by direct-current treatment in human leukemic ... Exposure of human leukemic cell lines (HL-60, ML-1, U-937, MOLT-4, EOL-1) to short direct-current (d.c.) treatment induced ...
We report the first identified mutation in the gene encoding human cytochrome c (CYCS). Glycine 41, invariant throughout ... A mutation of human cytochrome c enhances the intrinsic apoptotic pathway but causes only thrombocytopenia Nat Genet. 2008 Apr; ... The mutation yields a cytochrome c variant with enhanced apoptotic activity in vitro. Notably, the family has no other ... We report the first identified mutation in the gene encoding human cytochrome c (CYCS). Glycine 41, invariant throughout ...
... a series of benzochromene derivatives were screened for their cytotoxic activity against seven human cancer cell lines by ... Cytotoxic and apoptotic effects of these compounds in human cancer cells indicated that they can be a good candidate for ... The effects of compounds on reactive oxygen species (ROS) and nitric oxide (NO) generations in three human breast cancer cell ... 17693016 - Differential apoptotic induction of gambogic acid, a novel anticancer natural product, .... ...
Dual involvement of caspase-4 in inflammatory and ER stress-induced apoptotic responses in human retinal pigment epithelial ... Dual involvement of Caspase-4 in Inflammatory and ER Stress-induced Apoptotic Responses in Human Retinal Pigment Epithelial ... Dual involvement of Caspase-4 in Inflammatory and ER Stress-induced Apoptotic Responses in Human Retinal Pigment Epithelial ... Dual involvement of Caspase-4 in Inflammatory and ER Stress-induced Apoptotic Responses in Human Retinal Pigment Epithelial ...
Bacterial cell death induced by human pro-apoptotic Bax is blocked by an RNase E mutant that functions in an anti-oxidant ... We have shown that a trace amount of human Bax induces the cell death of Escherichia coli, accompanied by damage to DNA, and ... The N-terminal region of the RNase E protein inhibits bacterial death induced by human Bax as well as paraquat through a unique ...
Antiproliferative and Apoptotic Effects of a Specific Antiprostate Stem Cell Single Chain Antibody on Human Prostate Cancer ... F. Nejatollahi, S. J. Hodgetts, P. J. Vallely, and J. P. Burnie, "Neutralising human recombinant antibodies to human ... Human prostate cancer cell lines, DU-145 and LNCaP, were purchased from National Cell Bank of Iran (NCBI, Pasteur Institute of ... The apoptotic effect 33.80% in DU145 cells treated with C5-II versus the apoptosis 2.74% in untreated cells indicated the ...
In the present study, we examined the effects of resveratrol on apoptotic biochemical events in Hep G2 cells induced by CTN. ... Active JNK was required for CTN-induced mitochondria-dependent apoptotic biochemical changes, including loss of MMP, and ... and these apoptotic biochemical events are blocked by pretreatment with resveratrol, which exerts antioxidant effects. ... results collectively demonstrate that CTN stimulates ROS generation and JNK activation for mitochondria-dependent apoptotic ...
Cannabidiol induced a contrasting pro-apoptotic effect between freshly isolated and precultured human monocytes. Download Prime ... The present study investigated the pro-apoptotic effect of CBD on human peripheral monocytes that were either freshly isolated ... Cannabidiol induced a contrasting pro-apoptotic effect between freshly isolated and precultured human monocytes. Toxicol Appl ... Cannabidiol Induced a Contrasting Pro-apoptotic Effect Between Freshly Isolated and Precultured Human Monocytes. Toxicol Appl ...
Typical apoptotic characteristics, including chromatin condensation and fragmentation of nuclei, were observed and a high rate ... in the drug resistant human leukemia K562/A02 cells and further to explore potential mechanisms. After being incubated for 48 ... Apoptotic mechanism of human leukemia K562/A02 cells induced by magnetic iron oxide nanoparticles co-loaded with daunorubicin ... Apoptotic mechanism of human leukemia K562/A02 cells induced by magnetic iron oxide nanoparticles co-loaded with daunorubicin ...
Pro-apoptotic activity of α-bisabolol in preclinical models of primary human acute leukemia cells.. [Elisabetta Cavalieri, ... Our study provides the first evidence that α-bisabolol is a pro-apoptotic agent for primary human acute leukemia cells. ... We previously demonstrated that the plant-derived agent α-bisabolol enters cells via lipid rafts, binds to the pro-apoptotic ... α-bisabolol acted as a pro-apoptotic agent via a direct damage to mitochondrial integrity, which was responsible for the ...
This raises the possibility that the interaction of FFAs with PPARs can affect some key mechanisms of the human islet apoptotic ... We found that prolonged exposure to FFAs has cytostatic and pro-apoptotic effects on human pancreatic β-cells. The cytostatic ... Prolonged Exposure to Free Fatty Acids Has Cytostatic and Pro-Apoptotic Effects on Human Pancreatic Islets. Evidence that β- ... Thus, prolonged exposure to FFAs has cytostatic and pro-apoptotic effects on human pancreatic β-cells. The cytostatic action is ...
The Acetone Extract of Sclerocarya birrea (Anacardiaceae) Possesses Antiproliferative and Apoptotic Potential against Human ... as well as formation of apoptotic bodies. The acetone extract of S. birrea possesses antiproliferative and apoptotic potential ... Morphological examination also displayed apoptotic characteristics in the treated cells, including clumping, condensation, and ... The extract also exerted apoptotic programmed cell death in MCF-7 cells with significant effect on the DNA. ...
... we investigated the potential cytotoxicity and apoptotic effects of MA in human keratinocyte cell lines (HaCaT). The data ... To investigate the biological effects of MA in human skin keratinocytes, ... and sub-G1 phase in HaCaT cells and normal human epidermal keratinocytes (NHEKs). Flow cytometric assays also showed that MA ... Hsiao Y-P, Lai W-W, Wu S-B, Tsai C-H, Tang S-C, Chung J-G, Yang J-H. Triggering Apoptotic Death of Human Epidermal ...
Zhang H, Gao S and De Geyter C: A natural antisense transcript, BOKAS, regulates the pro-apoptotic activity of human Bok. Int J ... A natural antisense transcript, BOKAS, regulates the pro-apoptotic activity of human Bok. *Authors: *Hong Zhang ... Zhang, H., Gao, S., De Geyter, C.A natural antisense transcript, BOKAS, regulates the pro-apoptotic activity of human Bok. ... Zhang, H., Gao, S., De Geyter, C.A natural antisense transcript, BOKAS, regulates the pro-apoptotic activity of human Bok. ...
... Sloniecka, Marta Umeå University. ... Inhibition of the PKB/Aktpathway revealed that its activation is essential for mediating the anti-apoptotic effect of ACh in ... In the studies of this thesis, it is hypothesized that neuropeptides and neurotransmitters areproduced by human keratocytes and ... Cultured primary cells of the human corneal stroma expressed keratocyte markers (keratocan,lumican, CD34, and ALDH), the ...
These liposomes contained phosphatidylserine (PS) -the main signal of the apoptotic cell membrane- and β-cell autoantigens. To ... These liposomes contained phosphatidylserine (PS) -the main signal of the apoptotic cell membrane- and β-cell autoantigens. To ... PS accelerated phagocytosis of liposomes with a dynamic typical of apoptotic cell clearance, preserving DCs viability. After PS ... PS accelerated phagocytosis of liposomes with a dynamic typical of apoptotic cell clearance, preserving DCs viability. After PS ...
CD44 regulates phagocytosis of apoptotic neutrophil granulocytes, but not apoptotic lymphocytes, by human macrophages.. S P ... We demonstrate that phagocytosis of apoptotic neutrophils, but not apoptotic lymphocytes, by human monocyte-derived macrophages ... CD44 regulates phagocytosis of apoptotic neutrophil granulocytes, but not apoptotic lymphocytes, by human macrophages. ... CD44 regulates phagocytosis of apoptotic neutrophil granulocytes, but not apoptotic lymphocytes, by human macrophages. ...
Transforming Growth Factor β1 Induces Apoptotic Cell Death in Cultured Human Gastric Carcinoma Cells. Kazuyoshi Yanagihara and ... Transforming Growth Factor β1 Induces Apoptotic Cell Death in Cultured Human Gastric Carcinoma Cells ... Transforming Growth Factor β1 Induces Apoptotic Cell Death in Cultured Human Gastric Carcinoma Cells ... Transforming Growth Factor β1 Induces Apoptotic Cell Death in Cultured Human Gastric Carcinoma Cells ...
Selection of Human Cervical Epithelial Cells That Possess Reduced Apoptotic Potential to Low-Oxygen Conditions. Charlotte Y. ... Selection of Human Cervical Epithelial Cells That Possess Reduced Apoptotic Potential to Low-Oxygen Conditions ... Selection of Human Cervical Epithelial Cells That Possess Reduced Apoptotic Potential to Low-Oxygen Conditions ... Selection of Human Cervical Epithelial Cells That Possess Reduced Apoptotic Potential to Low-Oxygen Conditions ...
Induction of apoptosis in human NT2/D1 cells assessed as late apoptotic cells at 25 uM after 4 hrs by annexin V/propidium ...
Human alveolar epithelial cells engulf apoptotic eosi-nophils by means of integrin- and phosphatidylserine receptor dependent ... Sexton, D.W., Blaylock, M.G. and Walsh, G. (2001) Human alveolar epithelial cells engulf apoptotic eosi-nophils by means of ... The Apoptotic Effect of the Methanol Extract of Polygonum cuspidatum through Up-Regulation Death Receptor 5 and CHOP in HSC-2 ... Adherence of a Clinical Strain of Mycoplasma fermentans to Human Cultured Epithelial Cells ...
Modulation of the Apoptotic Response of Human Myeloid Leukemia Cells to a Diphtheria Toxin Granulocyte-Macrophage Colony- ... Modulation of the Apoptotic Response of Human Myeloid Leukemia Cells to a Diphtheria Toxin Granulocyte-Macrophage Colony- ... Modulation of the Apoptotic Response of Human Myeloid Leukemia Cells to a Diphtheria Toxin Granulocyte-Macrophage Colony- ... Modulation of the Apoptotic Response of Human Myeloid Leukemia Cells to a Diphtheria Toxin Granulocyte-Macrophage Colony- ...
  • We have shown that a trace amount of human Bax induces the cell death of Escherichia coli, accompanied by damage to DNA, and that the region of Bax which is lethal to E. coli is also responsible for apoptosis-inducing activity in the mammalian cells. (nih.gov)
  • Our results suggest that TGF-β 1 induces cell death in human gastric scirrhous carcinoma cells in vitro which is mediated by activation of a signal transduction pathway for apoptosis. (aacrjournals.org)
  • As a whole, this study defines COX-2 as a hitherto unknown target by which a cannabinoid induces apoptotic death of glioma cells. (aspetjournals.org)
  • Lipid-soluble ginseng extract induces apoptosis and G0/G1 cell cycle arrest in NCI-H460 human lung cancer cells. (qxmd.com)
  • Rottlerin induces autophagy and apoptotic cell death through a PKC-delta-independent pathway in HT1080 human fibrosarcoma cells: the protective role of autophagy in apoptosis. (qxmd.com)
  • Arenobufagin, a natural bufadienolide from toad venom, induces apoptosis and autophagy in human hepatocellular carcinoma cells through inhibition of PI3K/Akt/mTOR pathway. (qxmd.com)
  • Triptolide induces cell death in pancreatic cancer cells by apoptotic and autophagic pathways. (qxmd.com)
  • In vitro treatment of human prostate carcinoma cells with berberine inhibits the proliferation potential and induces cell death in a dose- and time-dependent manner. (aacrjournals.org)
  • In macrophages, engulfment of apoptotic bodies induces expression of transforming growth factor-β (TGF-β), a profibrogenic cytokine. (elsevier.com)
  • Conclusion: Punicalagin induces human U87MG glioma cell death through both apoptotic and autophagic pathways. (elsevier.com)
  • Mcl-1 protein expression was considerably enhanced in human HCC tissue compared to adjacent non-tumor tissue. (nih.gov)
  • The N-terminal region of the RNase E protein inhibits bacterial death induced by human Bax as well as paraquat through a unique mechanism that is distinct from RNA digestion. (nih.gov)
  • We previously demonstrated that the plant-derived agent α-bisabolol enters cells via lipid rafts, binds to the pro-apoptotic Bcl-2 family protein BID, and may induce apoptosis. (sigmaaldrich.com)
  • Moreover, loss of the mitochondrial membrane potential (MMP) was markedly induced by NAT-F. Additional results revealed that NAT-F could up-regulate pro-apoptotic protein Bax and down-regulate anti-apoptotic protein Bcl-2, Mcl-1, and Bcl-x L , resulting in cytochrome c release from mitochondria and sequential activation of caspase-9 and -3, as well as the cleavage of poly (ADP-ribose) polymerase. (frontiersin.org)
  • Apoptotic cell death induced by activation of the Fas/Fas-L system requires a multistep cascade of biochemical events: the trimerization of Fas receptor induced by Fas-L stimulates the formation of a death-inducing signaling complex, which consists of the adapter protein FADD and the protease FLICE/caspase-8. (aspetjournals.org)
  • The apoptotic induction potential was also studied by the analysis of Bcl-2 protein and gene expression in mace extract incubated KB cell lines using western blotting technique and real-time polymerase chain reaction. (scielo.br)
  • Induction of apoptotic cell death in human colorectal carcinoma cell lines by a cyclooxygenase-2 (COX-2)-selective nonsteroidal anti-inflammatory drug: independence from COX-2 protein expression. (aacrjournals.org)
  • The BAD protein is a pro-apoptotic member of the Bcl-2 family whose ability to heterodimerize with survival proteins such as Bcl-X(L) and to promote cell death is inhibited by phosphorylation. (elsevier.com)
  • Monoclonal antibodies were generated against the human BAD protein and used to evaluate its expression by immunoblotting and immunohistochemistry in normal human tissues and by immunoblot analysis of the National Cancer Institute anti-cancer drug screening panel of 60 human tumor cell lines. (elsevier.com)
  • The relative levels of BAD protein varied widely among established human tumor cell lines, with colon, lung, and melanomas generally having the highest expression. (elsevier.com)
  • The BAD protein derived from 11 of 41 tumor lines that expressed this pro-apoptotic protein migrated in gels as a clear doublet, consistent with the presence of hyperphosphorylated BAD protein. (elsevier.com)
  • Taken together, these findings define for the first time the normal cell-type-specific patterns of expression and intracellular locations of the BAD protein in vivo and provide insights into the regulation of this pro-apoptotic Bcl-2 family protein in human tumors. (elsevier.com)
  • Reed, John C. / Expression and location of pro-apoptotic bcl-2 family protein BAD in normal human tissues and tumor cell lines . (elsevier.com)
  • Jie Yang, " Molecular Modeling of Human BAD, a Pro-Apoptotic Bcl-2 Family Member, Integrating Glycolysis and Apoptosis", Protein & Peptide Letters (2010) 17: 206. (eurekaselect.com)
  • The synergistic apoptotic effects were supported by docking analysis, which demonstrated that panaxadiol and irinotecan bound two different chains of the caspase-3 protein. (qxmd.com)
  • Role of cyclin inhibitor protein p21 in the inhibition of HCT116 human colon cancer cell proliferation by American ginseng (Panax quinquefolius) and its constituents. (qxmd.com)
  • Changes (mRNA, protein) in expression of major proapoptotic p53, p21WAF1/CIP1, bax, bcl-Xs genes, and the antiapoptotic bcl-2 gene were observed in malignant MCF-7 and MDA-MB-231 cells and in benign MCF-10a human mammary tumor cells in culture. (elsevier.com)
  • It has been shown to inhibit activator protein 1, a key transcription factor in inflammation and carcinogenesis, in human cell lines ( 13 ) and has been shown to possess antitumor properties and effectively inhibit cyclooxygenase-2 transcriptional activity in human colon cancer cells ( 14 , 15 ). (aacrjournals.org)
  • Whether a cell undergoes apoptosis or continues through the cell cycle in response to potential apoptotic stimuli is under the control of a complex set of gene and protein interactions. (aacrjournals.org)
  • MicroRNA-22-3p (miR-22-3p), eIF4E-binding protein 3 (eIF4EBP3), Apoptosis, Human cervical squamous carcinoma cells. (medsci.org)
  • In contrast, blocking of the mitochondrial pathway of apoptosis either with a caspase-9 inhibitor or overexpressing the anti-apoptotic protein Bcl-2 (U937/Bcl-2) reduced the number of apoptotic cells in response to hyperthermia but it was unable to suppress melatonin enhancement. (uthscsa.edu)
  • BAG family molecular chaperone regulator 1 is a protein that in humans is encoded by the BAG1 gene. (wikipedia.org)
  • Thus, polypenolic-rich extracts of the non-edible parts of P. granatum were investigated for their antiproliferative and apoptotic effects on U266 multiple myeloma cells. (springer.com)
  • The antiproliferative and apoptotic induction effects were evaluated by MTT and Annexin-V assays, respectively. (hindawi.com)
  • The acetone extract of S. birrea possesses antiproliferative and apoptotic potential against MCF-7-treated cells and could be further exploited as a potential lead in anticancer therapy. (hindawi.com)
  • Zhang Z, Feng Y, Li Z, Cao X. Antiproliferative and apoptotic activity of glycyrrhizinic acid in MCF-7 human breast cancer cells and evaluation of its effect on cell cycle, cell migration and m-TOR/PI3K/Akt signalling pathway. (termedia.pl)
  • Data from this study suggested that caspase-3- and caspase-9-mediated apoptosis may play an important role in the panaxadiol enhanced antiproliferative effects of irinotecan on human colorectal cancer cells. (qxmd.com)
  • Exposure of human leukemic cell lines (HL-60, ML-1, U-937, MOLT-4, EOL-1) to short direct-current (d.c.) treatment induced apoptotic cell death, characterized by cell shrinkage and nuclear and internucleosomal DNA fragmentation. (springer.com)
  • Anti-apoptotic members of the Bcl-2 family, including myeloid cell leukemia-1 (Mcl-1), which regulate intrinsic apoptosis induction at the mito-chondrial level, are often overexpressed in human cancer, and are implicated with disease grade and prognosis. (nih.gov)
  • This induction of apoptotic caspases occurs exclusively in HIV-1-infected macrophages and not in bystander cells, leading to a significant reduction in HIV-1 replication and overall viral burden in the macrophage culture. (pnas.org)
  • Fujinaga S, Sakagami H, Kuribayashi N, Takahashi H, Amano Y, Sakagami T, Takeda M (1994) Possible role of hydrogen peroxide in apoptosis induction by ascorbic acid in human myelogenous leukemic cell lines. (springer.com)
  • Kuribayashi N, Sakagami H, Sakagami T, Niimi E, Shiokawa D, Ikekita M, Takeda M, Tanuma S (1994) Induction of DNA fragmentation in human myelogenous leukemic cell lines by sodium 5,6 benzylidene- l -ascorbate and its related compounds. (springer.com)
  • In conclusion, PS-liposomes phagocytosis mimics efferocytosis and leads to phenotypic and functional changes in human DCs, which are accountable for tolerance induction. (frontiersin.org)
  • The present study was conducted to evaluate cytotoxicity and apoptotic induction potential of Myristica fragrans Houtt mace extract. (scielo.br)
  • The present study investigated a possible relationship between the recently shown induction of cyclooxygenase (COX)-2 expression by the endocannabinoid analog R (+)methanandamide [R(+)-MA] and its effect on the viability of H4 human neuroglioma cells. (aspetjournals.org)
  • We here investigated induction of apoptosis in human breast cancer cells (MCF7 cell line) by an IgY produced against an 21 aminoacid epitope of the human TRAIL-2 receptor. (waocp.org)
  • Increased anticancer activity of the thymidylate synthase inhibitor BGC9331 combined with the topoisomerase I inhibitor SN-38 in human colorectal and breast cancer cells: induction of apoptosis and ROCK cleavage through caspase-3-dependent and -independent mechanisms. (qxmd.com)
  • Here we found that ROT induced autophagy followed by induction of apoptosis via inhibition of PI3K/Akt/mTOR pathway and activation of caspase cascade in human pancreatic CSCs. (qxmd.com)
  • We also demonstrate that IL-2 and IL-15 suppress up-regulation of Bim and induction of a death receptor-independent apoptotic program triggered by partially agonistic APL. (ki.se)
  • It is unknown whether prolonged exposure to FFAs affects the survival of isolated human islet cells and, if so, which pathways are involved. (diabetesjournals.org)
  • Previously defined inhibitors of apoptotic cell recognition did not affect CD44-augmented phagocytosis of apoptotic neutrophils, suggesting that unique molecular recognition pathways are involved. (jimmunol.org)
  • Most studies showed that cigarette smoke inhibits cell proliferation through these apoptotic pathways, whereas there have been some reports that are contrary [ 8 , 13 , 14 ]. (medsci.org)
  • Findings indicate that CLA elicits mainly proapoptotic effects in human breast tumor cells through both p53-dependent and p53-independent pathways, according to cell type. (elsevier.com)
  • We conclude that DT388-GM-CSF can kill acute myeloid leukemia blasts but that apoptotic sensitivities will depend on the presence of at least 100 high affinity GM-CSF receptors/cell and the absence of overexpressed antiapoptotic proteins. (bloodjournal.org)
  • Furthermore, the results of xenograft assay suggested that marginatoxin inhibited the BEL-7402 cells-induced tumor growth without effect on body weight of nude mice in vivo by regulating the expression levels of above apoptotic proteins. (alliedacademies.org)
  • As ApoBDs can harbour DNA, microRNA and proteins, the disassembly of an apoptotic cell into ApoBDs can mediate intercellular communication through the transport of these biomolecules and may contribute to the development of various disease states 2 , 3 . (nature.com)
  • Mechanisms leading to apoptosis were also investigated at the molecular level by measuring the amount of anti and pro-apoptotic proteins and at the cellular level by studying DNA fragmentation and annexin V staining by flow cytometry. (koreascience.or.kr)
  • This anti proliferative activity was associated with an increase of apoptosis as demonstrated by DNA fragmentation, the appearance of apoptotic cells in flow cytometry analysis and an increase of the amount of calpain 1, calpastatin, caspase 3, caspase 9, Bax and Bcl-2, all proteins involved in the apoptotic pathway. (koreascience.or.kr)
  • It contains anti-apoptotic proteins, which promote cell survival, and pro-apoptotic ones, which induce cell death through the release of apoptotic factors from mitochondria. (ki.se)
  • Bim, as well as Bid were shown to bind the majority of anti-apoptotic and proapoptotic Bcl-2 family members and are believed to induce oligomerization of Bax and Bak proteins, which results in the outer membrane permeabilization and release of pro-apoptotic factors initiating cell death. (ki.se)
  • Despite the increased expression ofBAG-1, the balance between pro- and anti-apoptotic factors shifted towards increased expression of pro-apoptotic proteins. (mun.ca)
  • On the other hand, human peripheral blood lymphocytes and polymorphonuclear cells were relatively resistant to d.c treatment, and did not show any clear nuclear and DNA fragmentation. (springer.com)
  • Typical apoptotic characteristics, including chromatin condensation and fragmentation of nuclei, were observed and a high rate of apoptosis was detected in K562/A02 cells treated with DNR/Br Tet-MNPs and DNR/Br Tet-Sol. (dovepress.com)
  • Morphological examination also displayed apoptotic characteristics in the treated cells, including clumping, condensation, and culminating to budding of the cells to produce membrane-bound fragmentation, as well as formation of apoptotic bodies. (hindawi.com)
  • The data showed that MA induced apoptosis based on the observations of DAPI staining, DNA fragmentation, and sub-G1 phase in HaCaT cells and normal human epidermal keratinocytes (NHEKs). (mdpi.com)
  • Caspase-3, Neutral red and DNA fragmentation assays were carried out on HeLa cell lines cultured in Dulbecco s Modified Eagles Medium (DMEM) in (95% O 2 + 5% CO 2 ) at 35°C. The apoptotic, cytotoxic capacities and DNA fragmentation assays were carried out on the medicinal plant s. (scialert.net)
  • Pretreatment with the pan-caspase inhibitor z-VAD-fmk partially, but significantly, blocked the berberine-induced apoptosis, as also confirmed by the comet assay analysis of DNA fragmentation, suggesting that berberine-induced apoptosis of human prostate cancer cells is mediated primarily through the caspase-dependent pathway. (aacrjournals.org)
  • Apoptotic effect was confirmed by morphological changes, DNA fragmentation and caspase activation assay. (mahidol.ac.th)
  • Apoptotic cells were evaluated using PI staining of DNA fragmentation by flow cytometry (sub-G1 peak). (ac.ir)
  • DNA fragmentation indicating apoptotic cell death was involved in R. turkestanicum induced toxicity and cleaved PARP fragment was also detected. (ac.ir)
  • Previously described receptors on blood- derived macrophages have been characterized in the absence of serum and show a relatively low uptake of apoptotic cells. (rupress.org)
  • We report that a serum factor(s) provides a more than threefold increase in the uptake of apoptotic cells by human macrophages and demonstrate that complement components are required for this high level of uptake. (rupress.org)
  • Interaction between human macrophages and apoptotic cells was performed as described (( 3 ), ( 14 )) with minor modifications in the presence or absence of 15% human serum. (rupress.org)
  • Human macrophages were isolated from peripheral blood monocytes of normal donors as described (( 14 )) and cultured on Chamber-Tek glass slides (Nunc, Inc., Naperville, IL). (rupress.org)
  • Here, we have screened a panel of 90 long noncoding RNAs (lncRNA) and identified a lncRNA, SAF, that plays a critical role in the resistance of HIV-1-infected macrophages to activation of apoptotic caspases. (pnas.org)
  • Here, we have identified the impact of the lncRNA SAF in regulating apoptotic effector caspases in macrophages, a long-lived cellular reservoir of HIV-1, that are largely immune to virus-induced cell death. (pnas.org)
  • Expression of SAF is significantly up-regulated in HIV-1-infected human monocyte-derived macrophages (MDM) compared with bystander and virus-nonexposed cells. (pnas.org)
  • CD44 regulates phagocytosis of apoptotic neutrophil granulocytes, but not apoptotic lymphocytes, by human macrophages. (jimmunol.org)
  • Phagocytosis of apoptotic neutrophil granulocytes by macrophages at inflammatory sites is an important determinant of the process by which inflammation resolves. (jimmunol.org)
  • We demonstrate that phagocytosis of apoptotic neutrophils, but not apoptotic lymphocytes, by human monocyte-derived macrophages is augmented rapidly following ligation of CD44 by bivalent Abs in vitro. (jimmunol.org)
  • Apoptotic and inflammatory effects induced by different particles in human alveolar macrophages. (semanticscholar.org)
  • Combined effects of fine particulate matter and lipopolysaccharide on apoptotic responses in NR8383 macrophages. (semanticscholar.org)
  • Here we investigated the role of peroxynitrite in apoptosis occurring in astroglial cells incubated with supernatants of HIV-infected human primary macrophages (M/M). (biomedcentral.com)
  • Recently, it has been shown that astroglial apoptosis play a key role in the neurological progression of HIV-1 associated dementia [ 4 ] and furthermore, incubation of astroglial cells with supernatants of HIV-infected macrophages leads to astrocytic oxidative stress and then to apoptotic cell death. (biomedcentral.com)
  • PS, also expressed on apoptotic cells (ACs) enables macrophages to phagocytose ACs. (westminster.ac.uk)
  • 1 μmol/L). Growth suppression of INA-6 correlated with an increase in the percentage of apoptotic cells and inhibition of signal transducer and activator of transcription 3 phosphorylation. (aacrjournals.org)
  • These data suggest that targeting this CD44-mediated inhibition by apoptotic microparticles could be a novel strategy to potentiate DC activation of HIV-specific immunity. (ox.ac.uk)
  • CONCLUSION: LL-37 strongly attenuates lipopolysaccharide-induced cytokine and chemokine expression and, in high concentrations, reduces cell proliferation through inhibition of DNA synthesis and by promoting apoptosis in human periodontal ligament cells. (mau.se)
  • These results documented the cytotoxic and apoptotic effects of P. granatum extracts on human U266 multiple myeloma cells via disruption of mitochondrial membrane potential and increasing cell cycle arrest. (springer.com)
  • Cytotoxic and apoptotic effects of synthetic benzochromene derivatives on human cancer cell lines. (biomedsearch.com)
  • Cytotoxic and apoptotic effects of these compounds in human cancer cells indicated that they can be a good candidate for further pharmacological studies to discover effective anticancer agents. (biomedsearch.com)
  • P. granatum extracts had cytotoxic and apoptotic effects on human U266 multiple myeloma cells via disruption of mitochondrial membrane potential and increasing cell cycle arrest. (greenmedinfo.com)
  • Bacterial cell death induced by human pro-apoptotic Bax is blocked by an RNase E mutant that functions in an anti-oxidant pathway. (nih.gov)
  • The cytostatic action was accompanied by reduced intraislet glucose metabolism, and the pro-apoptotic effects were mostly, but not solely, caspase mediated, partially dependent on ceramide pathway, and possibly Bcl-2 regulated. (diabetesjournals.org)
  • The delineation of discrete apoptotic pathway has affected not only our basic concepts of the development of cancer, but also our approaches towards the prevention and therapy of the disease ( Maryam, Nadareh, Hossein , 2013 ). (scielo.br)
  • Recent studies from our laboratory have shown that cannabinoids induce the expression of the cyclooxygenase (COX)-2 enzyme in human neuroglioma cells via a cannabinoid- and vanilloid receptor-independent pathway involving increased synthesis of ceramide (Ramer et al. (aspetjournals.org)
  • Recently, pathway analysis identified cyclin-dependent kinase 1 (CDK1) upregulation in a subset of human BRAF V600E colorectal cancers. (aacrjournals.org)
  • These results demonstrated that marginatoxin could induce apoptosis of human hepatoma BEL-7402 cells in vitro and in vivo through activation of Fas/FasL-mediated apoptotic pathway. (alliedacademies.org)
  • Furthermore, the influence of histone deacetylase inhibitors (HDIs) on the NFkB pathway in human non-small cell lung cancer (NSCLC) cell lines was investigated. (weltbild.de)
  • In conclusion, these data support a model in which engulfment of hepatocyte apoptotic bodies by stellate cells leads to a fibrogenic response by eliciting a kinase-signaling pathway. (elsevier.com)
  • Raybiotech Inc C-Series Human Apoptosis Signaling Pathway Array C1. (fishersci.com)
  • Melatonin provokes cell death in human B-lymphoma cells by mitochondrial-dependent apoptotic pathway activation. (univpm.it)
  • Dual involvement of caspase-4 in inflammatory and ER stress-induced apoptotic responses in human retinal pigment epithelial cells. (nih.gov)
  • These observations, together with the lack of effect of CD44 Abs upon macrophage phagocytosis of zymosan or Ig-opsonized erythrocytes, imply that CD44 may regulate the differential clearance of apoptotic leukocytes during evolution of inflammatory responses. (jimmunol.org)
  • Despite a considerable number of in vitro studies, the relationship among these cells, as well as the factors that control their immigration, differentiation, and proliferation under homeostatic and inflammatory conditions, remains unclear in humans. (rupress.org)
  • Here, we assess anti-inflammatory properties of LL-37 and investigate the effects of LL-37 on cell differentiation, cell proliferation and apoptosis in human periodontal ligament cells. (mau.se)
  • Stellate cells, but not hepatocytes, readily engulfed apoptotic bodies in a time-dependent manner as assessed by confocal microscopy. (elsevier.com)
  • Within the scope of this trial, we investigated the effects of the natural pine bark extract pycnogenol on human fibrosarcoma cells. (spandidos-publications.com)
  • The effects of compounds on reactive oxygen species (ROS) and nitric oxide (NO) generations in three human breast cancer cell lines were also studied. (biomedsearch.com)
  • In the present study, we examined the effects of resveratrol on apoptotic biochemical events in Hep G2 cells induced by CTN. (mdpi.com)
  • These results collectively demonstrate that CTN stimulates ROS generation and JNK activation for mitochondria-dependent apoptotic signaling in Hep G2 cells, and these apoptotic biochemical events are blocked by pretreatment with resveratrol, which exerts antioxidant effects. (mdpi.com)
  • In an effort to better understand the phenomenon of lipotoxicity in human β-cells, we evaluated the effects of 48-h preculture with 1.0 or 2.0 mmol/l free fatty acid (FFA) (2:1 oleate to palmitate) on the function and survival of isolated human islets and investigated some of the possible mechanisms. (diabetesjournals.org)
  • Thus, prolonged exposure to FFAs has cytostatic and pro-apoptotic effects on human pancreatic β-cells. (diabetesjournals.org)
  • To investigate the biological effects of MA in human skin keratinocytes, we investigated the potential cytotoxicity and apoptotic effects of MA in human keratinocyte cell lines (HaCaT). (mdpi.com)
  • The aims of this thesis project were to test these hypotheses and to study the underlying inter- andintracellular mechanisms of the effects of SP and ACh on keratocytes.Results: Cultured primary cells of the human corneal stroma expressed keratocyte markers (keratocan,lumican, CD34, and ALDH), the tachykinins SP and NKA, catecholamines (adrenaline, noradrenaline anddopamine), ACh, and glutamate. (diva-portal.org)
  • These pathogenic effects of cigarette smoke on diverse human tissues and cells are mainly from the multiple toxic compounds present in cigarette smoke. (medsci.org)
  • Human endothelial cells were treated with monocytic microparticles and then, we studied their effects on nitrosative and oxidative stresses. (surrey.ac.uk)
  • In this study, we produced microparticles in vitro from apoptotic human monocytes and examined the effects of their interaction with cultured human umbilical vascular endothelial cells (HUVECs). (inserm.fr)
  • Indeed, PCP4/PEP19 has been reported to have anti-apoptotic effects on rat chromaffin PC12 and HEK293T cells [ 9 - 11 ]. (oncotarget.com)
  • In this study, we have investigated the possible synergistic anticancer effects of panaxadiol and irinotecan on human colorectal cancer cells and explored the potential role of apoptosis in their synergistic activity. (qxmd.com)
  • Panaxadiol, a purified ginseng component, enhances the anti-cancer effects of 5-fluorouracil in human colorectal cancer cells. (qxmd.com)
  • Subsequently, we stimulated cultured human tm- CXCL16-positive, CXCR6-negative meningioma cells with recombinant s- CXCL16 and analyzed binding, signaling and biological effects using RNAi silencing to verify specificity. (biomedcentral.com)
  • The aim of the study was to investigate the effects of glucocorticoids in cultured human lens epithelial cells. (molvis.org)
  • Effects on proliferation and/or dysregulation of apoptosis in lens epithelial cells may be an important factor in human steroid-induced posterior subcapsular cataract. (molvis.org)
  • Aim: To investigate the effects of punicalagin, a polyphenol isolated from Punica granatum, on human U87MG glioma cells in vitro. (elsevier.com)
  • Apoptotic Effects of Linum album Extracts on AGS Human Gastric Adenocarcinoma Cells and ZNF703 Oncogene Expression', Asian Pacific Journal of Cancer Prevention , 19(10), pp. 2911-2916. (waocp.org)
  • It enhances the anti-apoptotic effects of BCL2 and represents a link between growth factor receptors and anti-apoptotic mechanisms. (wikipedia.org)
  • Chudomel V, Soucek J, Hruba A, Jerabek J, Schwarz J, Smetana K (1989) Positive effect of direct current on cytotoxicity of human lymphocytes. (springer.com)
  • Cell suspensions enriched in cells at various stages of apoptosis were obtained by separation of irradiated human peripheral blood lymphocytes on density gradients at different post-irradiation times. (diva-portal.org)
  • Polygonaceae) root were determined against HeLa and MCF-7 cell lines and human blood lymphocytes. (ac.ir)
  • The focus of this work was to analyze how different modes of TCR-triggering affect Bim expression in human T-cells at different stages of their differentiation with particular emphasis on effector CD8+ cytotoxic T-lymphocytes (CTLs). (ki.se)
  • We analyzed the expression of Bim in total peripheral blood lymphocytes (PBLs) and different subpopulations of ex vivo isolated human T-lymphocytes from healthy donors or patients with infectious mononucleosis. (ki.se)
  • In this study, we used DNA microarray profiling to investigate changes in the expression of genes involved in apoptosis that occur in human idiopathic dilated cardiomyopathic hearts that had progressed to heart failure. (rti.org)
  • Gene expression profiles of ovarian cancer cells treated with ACFP were generated by cDNA microarray, and the expression of apoptotic-specific genes, such as bcl -xl, bax , akt , caspase -3, CDC 25C and cyclin B1, was assessed by real time PCR and western blot analysis. (biomedcentral.com)
  • 5 ] found that this compound induced tumor cell differentiation by modulating the expression of genes implicated in tumor metastasis and immunogenicity in cultured human melanoma cells. (biomedcentral.com)
  • The genes were identified by studies in the nematode C. elegans and homologues of these genes function in humans to regulate apoptosis. (wikipedia.org)
  • Our findings indicate that hESCs express elevated levels of the pro-apoptotic BH3-only BCL-2 family members NOXA, BIK, BIM, BMF and PUMA when compared with differentiated cells and cancer cells. (ca.gov)
  • Conditions that limit clearance of apoptotic bodies (e.g., age-associated declines in immune competence) would be predicted to accelerate the process. (neurodegenerationresearch.eu)
  • Several ligands and receptors have been reported to play a role in the initial engulfment of apoptotic cells in vitro. (rupress.org)
  • The extract also exerted apoptotic programmed cell death in MCF-7 cells with significant effect on the DNA. (hindawi.com)
  • The cytotoxic activity of the Myristica fragrans Houtt mace acetone extract was assayed by MTT assay on human oral epidermal carcinoma KB cell lines. (scielo.br)
  • The apoptotic potential of mace extract was accompanied by reduced gene expression of Bcl-2 compared to the untreated KB cells. (scielo.br)
  • Conclusively, the aqueous extract of Ganoderma lucidum proves to be suitable for the development of an antitumour agent as shown by its apoptotic effect reported in this study. (scialert.net)
  • In addition, concomitant treatment of the MCF-7 cells with SKE and vincristine produced a potent anti-proliferative and pro-apoptotic effect compared to extract or drug alone. (ac.ir)
  • In conclusion, satureja extract has a potential anti-cancer property against human breast cancer cells and its combination with chemotherapeutic agent vincristine may induce cell death effectively and be a potent modality to treat this type of cancer. (ac.ir)
  • The anti oxidant activity of an aqueous extract of Urtica dioica leaf was measured by MTT assay and the FRAP method while its anti-proliferative activity on the human breast cancer cell line (MCF-7) and fibroblasts isolated from foreskin tissue was evaluated using MTT assay. (koreascience.or.kr)
  • Material and Methods: Human colon adenocarcinoma cells HT29 were treated with different doses (1, 5 and 10 mg/ml) of fresh garlic extract in cell culture at 24, 48 and 72 h. (iranpath.org)
  • Conclusion: Fresh garlic extract can induce a dose-dependent apoptotic cell death in cultured HT29 cell line. (iranpath.org)
  • Garlic Extract Can Induce Apoptotic Cell Death in The Human Colon Adenocarcinoma HT29 Cell Line', Iranian Journal of Pathology , 5(3), pp. 126-131. (iranpath.org)
  • Azizzadeh Delshad, A., Heshmati, M., Ghaini, M. Garlic Extract Can Induce Apoptotic Cell Death in The Human Colon Adenocarcinoma HT29 Cell Line. (iranpath.org)
  • Cytotoxic and Apoptotic Potential of Rheum turkestanicum Janisch Root Extract on Human Cancer and Normal Cells', Iranian Journal of Pharmaceutical Research , 12(4), pp. 811-819. (ac.ir)
  • We observed distinct size and morphological differences of 30 to 100 nm of exosomes and apoptotic bodies through TEM analysis. (nature.com)
  • Apoptotic bodies (ApoBDs) are membrane-bound extracellular vesicles that can mediate intercellular communication in physiological and pathological settings. (nature.com)
  • Fluorescently labeled hepatocyte apoptotic bodies were added to cultures of primary and immortalized human stellate cells. (elsevier.com)
  • The activation of primary and immortalized human stellate cells after incubation with apoptotic bodies, as well as their fibrogenic activity, was indicated by an increase in α-smooth muscle actin (primary cells), TGF-β1, and collagen α1(I) mRNA (primary and immortalized cells). (elsevier.com)
  • An etoposide -treated DU145 prostate cancer cell exploding into a cascade of apoptotic bodies. (wikipedia.org)
  • Unlike necrosis, apoptosis produces cell fragments called apoptotic bodies that phagocytic cells are able to engulf and quickly remove before the contents of the cell can spill out onto surrounding cells and cause damage to the neighboring cells. (wikipedia.org)
  • Addition of serum to the phagocytosis assays increased the uptake of apoptotic cells by more than threefold. (rupress.org)
  • An important stage of apoptosis involves the acquisition of surface changes on the apoptotic cell leading to rapid recognition and phagocytosis by surrounding cells followed by degradation in lysosomes. (rupress.org)
  • To move toward a clinical application, PS-liposomes with optimum size and composition for phagocytosis were loaded with human insulin peptides and tested on DCs from patients with T1D and control age-related subjects. (frontiersin.org)
  • PS accelerated phagocytosis of liposomes with a dynamic typical of apoptotic cell clearance, preserving DCs viability. (frontiersin.org)
  • The present study investigated the pro-apoptotic effect of CBD on human peripheral monocytes that were either freshly isolated or precultured for 72h. (unboundmedicine.com)
  • Collectively, our results demonstrated a contrasting pro-apoptotic effect of CBD between precultured and freshly isolated monocytes, which was closely associated with the cellular level of glutathione and the antioxidative capability of the cells. (unboundmedicine.com)
  • TY - JOUR T1 - Cannabidiol induced a contrasting pro-apoptotic effect between freshly isolated and precultured human monocytes. (unboundmedicine.com)
  • Microparticles from apoptotic monocytes enhance nitrosative stress in human endothelial cells. (surrey.ac.uk)
  • Microparticles from apoptotic monocytes induce transient platelet recruitment and tissue factor expression by cultured human vascular endothelial cells via a redox-sensitive mechanism. (inserm.fr)
  • Treatment of nonneoplastic human prostate epithelial cells (PWR-1E) with berberine under identical conditions did not significantly affect their viability. (aacrjournals.org)
  • B to E, dose- and time-dependent effect of berberine on the viability of human prostate carcinoma cells and nonneoplastic human prostate epithelial (PWR-1E) cells. (aacrjournals.org)
  • Methods: The viability of human U87MG glioma cells was evaluated using MTT assay. (elsevier.com)
  • Cell death was assessed by viability test, and its apoptotic nature was determined by terminal deoxynucleotidyltransferase-mediated dUTP nick end labeling (TUNEL) assay. (iranpath.org)
  • The apoptosis analyses by TUNEL assay demonstrated that in each dose/time group the apoptotic values are much less prominent than the related cell death percentage found in viability test. (iranpath.org)
  • The effect of generated polyclonal antibodies on viability and apoptosis of human epidermoid carcinoma derived A431 cell line was assessed using MTT and Annexin V-propidium iodide assays. (exp-oncology.com.ua)
  • enhances the apoptotic response to antineoplastic drugs. (aspetjournals.org)
  • Asian ginseng enhances the anti-proliferative effect of 5-fluorouracil on human colorectal cancer: comparison between white and red ginseng. (qxmd.com)
  • Overexpression of BOKAS alone exhibited no significant anti- or pro-apoptotic activity but it was able to inhibit Bok-induced apoptosis in HeLa cells. (spandidos-publications.com)
  • HIV-1 infection-induced apoptotic microparticles inhibit human DCs via CD44. (ox.ac.uk)
  • miR-526a regulates apoptotic cell growth in human carcinoma cells. (sigmaaldrich.com)
  • Zhang H, Gao S and De Geyter C: A natural antisense transcript, BOKAS, regulates the pro-apoptotic activity of human Bok. (spandidos-publications.com)
  • The synergistic apoptotic interaction of panaxadiol and epigallocatechin gallate in human colorectal cancer cells. (qxmd.com)
  • However, with prolonged contact to 1 μM cisplatin or upon 24 hours exposure to 5 μM cisplatin, Xrel3 had a synergistic apoptotic effect when coupled with cisplatin, thus promoting chemosensitivity and cell death. (mun.ca)
  • This study was designed to investigate the detailed mechanism of its cytotoxic effect on human hepatoma BEL-7402 cells by CCK-8, flow cytometry, western blot and xenograft assays. (alliedacademies.org)
  • 3 ] reported that the anti-cancer bioactive peptide (ACBP), purified from goat spleens that were immunized with human gastric cancer extracts, significantly inhibited gastric cancer cell growth in vitro and gastric tumor growth in vivo, indicating that therapeutic peptides may represent a powerful anti-cancer tool. (biomedcentral.com)
  • The present study concerns the effect of different extracts of L. album on ZNF703 gene expression and apoptosis in human gastric carcinoma AGS cells. (waocp.org)
  • Since human papillomavirus (HPV) infection is strongly associated with cervical neoplasia and tumor hypoxia has prognostic significance in human cervical carcinomas, we examined the relationship between hypoxia and apoptosis in human cervical epithelial cells expressing high-risk HPV type 16 oncoproteins. (aacrjournals.org)
  • Using conventional hybridoma technology, not only are fully human monoclonal IgM antibodies isolated, but also new tumor-related targets can be identified using the same experimental approach. (iospress.com)
  • By studying now benign human tumor cells derived from the linings of the brain and spinal cord, so called meningiomas, Hattermann et al. (biomedcentral.com)
  • The Burkitt B cell line, BL-41, and normal human mature peripheral T cells were rendered apoptotic by γ-irradiation (4,000 cGy) followed by incubation in the absence of serum as for human neutrophils. (rupress.org)
  • Incubation of human endothelial cells with microparticles enhanced the production of NO without affecting superoxide anions generation. (surrey.ac.uk)
  • Here we examined the effect of TGF-β 1 on the growth of these lines as compared to five other human gastric adenocarcinoma cell lines. (aacrjournals.org)
  • In this report, we examined this adverse effect of cigarette smoke on human gingival fibroblasts (HGFs) which are critical not only in maintaining gingival tissue architecture but also in mediating immune responses. (medsci.org)
  • We examined the effect of black raspberry (BR) phenolics on cell proliferation of two phenotypically diverse human cancer cell lines isolated from patients with different stages and grades of transitional cell carcinoma. (osu.edu)
  • Subsequently, the anticancer effect of such IgYs was determined by MTT assay in the MCF7 human breast cancer cell line. (waocp.org)
  • Apoptotic effect of vinorelbine on human. (gazi.edu.tr)
  • Flow cytometric analysis (FACS) of human cultured astrocytes shortly incubated with HIV-1-infected M/M supernatants showed apoptotic cell death, an effect accompanied by pronounced staining for nitrotyrosine (footprint of peroxynitrite) and by abnormal formation of malondialdehyde (MDA). (biomedcentral.com)
  • The present experiments have been performed in order to evaluate the role of peroxynitrite in the apoptotic cell death of astroglial cells incubated with supernatants of HIV-1-infected M/M and ascertain the protective effect of FeTMPS, a novel peroxynitrite decomposition catalyst on HIV-1-related apoptosis of astroglial cells. (biomedcentral.com)
  • In this work, we examine the effect of melatonin on RAMOS-1 human leukaemic cells. (univpm.it)
  • The results suggest that the apoptotic effect of melatonin is associated with cell-cycle arrest, downregulation of Bcl-2, mitochondrial membrane depolarization, cytochrome c release and activation of caspase-3. (univpm.it)
  • In this study, we investigated the effect of XIAP down-regulation by antisense oligonucleotides (AS ODNs) on human non-small cell lung cancer (NIH-H460) growth in vitro and in vivo . (aacrjournals.org)
  • These results suggested that Xrel3, a homolog of c-Rel, causes an anti-apoptotic effect (chemoresistance) when cells are initially exposed to low cisplatin concentrations (1 μM). (mun.ca)
  • In this study, the effect of melatonin on hyperthermia-induced apoptosis was explored using human leukemia cells. (uthscsa.edu)
  • The potentiation of cell death was achieved with 1 mmol/L concentrations of the indoleamine but not with concentrations close to physiological levels in blood (1 nmol/L). This effect was associated to an enhancement of the apoptotic response, revealed by an increase in cells with hypodiploid DNA content and activation of multiple caspases (caspase-2, caspase-3, caspase-8, and caspase-9). (uthscsa.edu)
  • The aim of this study was to investigate the apoptotic effect of a proteasome inhibitor MG-132 on Tca-8113, a cell line of human tongue squamous cell carcinoma. (edu.au)
  • The mechanisms whereby apoptotic cells are efficiently identified, removed, and degraded by phagocytes in mammalian cells are not well understood. (rupress.org)
  • The purpose of this study was to assess the induced apoptosis of self-assembled iron oxide magnetic nanoparticles (MNPs) co-loaded with daunorubicin (DNR) and 5-bromotetrandrin (Br Tet) (DNR/Br Tet-MNPs), acting as a drug depot system for the sustained release of the loaded DNR and BrTet, in the drug resistant human leukemia K562/A02 cells and further to explore potential mechanisms. (dovepress.com)
  • Sexton, D.W., Blaylock, M.G. and Walsh, G. (2001) Human alveolar epithelial cells engulf apoptotic eosi-nophils by means of integrin- and phosphatidylserine receptor dependent mechanisms A process upregulated by dexa- me-thasone. (scirp.org)
  • 8 Thus, there is a need for new reagents with unique mechanisms of action that can selectively modify the apoptotic threshold of malignant blasts. (bloodjournal.org)
  • In the present study, we investigated the mechanisms of cell death induced by doxorubicin (DXR) and the novel disaccharide anthracycline MEN 10755, in a human ovarian cancer cell line (A2780). (aspetjournals.org)
  • Oxidative stress has shown to contribute in the mechanisms underlying apoptotic cell death occuring in AIDS-dementia complex. (biomedcentral.com)
  • What transformed cell death from obscurity to a major field of research were two things: the identification of components of the cell death control and effector mechanisms, and the linkage of abnormalities in cell death to human disease, in particular cancer. (wikipedia.org)
  • To investigate the functional involvement of caspase-4 in human retinal pigment epithelial (hRPE) cells. (nih.gov)
  • Although the process of long-term cell culturing resulted in selection for subpopulations of HPV oncoprotein-expressing cervical epithelial cells with diminished apoptotic potential, the exposure of cells to hypoxia greatly accelerated the selection process. (aacrjournals.org)
  • Human lens epithelial cells (HLECs) were exposed to dexamethasone for 24 h. (molvis.org)
  • α-bisabolol acted as a pro-apoptotic agent via a direct damage to mitochondrial integrity, which was responsible for the decrease in NADH-supported state 3 respiration and the disruption of the mitochondrial membrane potential. (sigmaaldrich.com)
  • ex DC (Apiaceae), showed cytotoxic activity against human hepatocellular carcinoma. (alliedacademies.org)
  • On THP-1 cells 1 induced powerful apoptotic response (ED 50 = 10 ± 3 μM), which was revealed to be only partially caspase-dependent, with activation of caspase-8 as the dominant course. (rsc.org)
  • AY4 as a single agent induced caspase-dependent apoptotic cell death of KB and HN9, but not in SNU899 and FaDu cell lines. (eurekamag.com)
  • These features are generally associated with apoptotic processes. (aacrjournals.org)
  • Thus, the study of the ontogeny of APCs during human intrauterine development provides a unique opportunity to evaluate these processes as well as their relationship in this human in vivo model. (rupress.org)
  • In addition to its importance as a biological phenomenon, defective apoptotic processes have been implicated in a wide variety of diseases. (wikipedia.org)
  • With the aim of discovering potential cytotoxic agents, a series of benzochromene derivatives were screened for their cytotoxic activity against seven human cancer cell lines by standard 3-(4, 5-dimethyl thiazol)-2,5-diphenyl tetrazolium bromide (MTT) assay. (biomedsearch.com)
  • Although caspase-4 inhibitor Z-LEVD-fmk and caspase-1 and -4 inhibitor Z-YVAD-fmk reduced tunicamycin-induced hRPE apoptotic cell death by 59% and 86%, respectively, pan-caspase inhibitor Z-VAD-fmk completely abolished the induced apoptosis. (nih.gov)
  • Cells were also protected from apoptotic cell death by other COX-2 inhibitors (NS-398 {[ N -[2-(cyclohexyloxy)-4-nitrophenyl]-methanesulfonamide]} and diclofenac) and by the ceramide synthase inhibitor fumonisin B 1 , which interferes with COX-2 expression by R(+)-MA. (aspetjournals.org)
  • ROS generation induced by AY4 treatment triggered down-regulation of anti-apoptotic molecules of Bcl-xL and X-linked inhibitor of apoptosis (XIAP) without affecting the expression levels of DR4, Mcl-1, and survivin. (eurekamag.com)
  • Although cell and animal models suggest a role for altered gene expression in the transition to heart failure, there is a paucity of data derived from the study of human heart tissue. (rti.org)
  • The NFkB activation status was investigated in human lung cancer, pancreatic cancer, and hematopoietic cancer cell lines. (weltbild.de)
  • The mutation yields a cytochrome c variant with enhanced apoptotic activity in vitro. (nih.gov)
  • Pro-apoptotic activity of α-bisabolol in preclinical models of primary human acute leukemia cells. (sigmaaldrich.com)
  • Although treatment of those cells with 1 resulted in poor apoptotic response, cell cycle changes showed concentration-dependent shifts indicating a dual mechanism of activity. (rsc.org)
  • In this study, we analyzed the anticancer activity according to the antiproliferation and apoptotic activity of the pure constituent isolated from S. venosa tuber on human ovarian cancer cells (SKOV3). (mahidol.ac.th)
  • It has pro-apoptotic activity. (wikipedia.org)
  • We used quantitative PCR to compare the steady-state expression profile of all human BCL-2 family members in hESCs with that of human primary cells from various origins and two cancer lines. (ca.gov)
  • The Cancer Genome Atlas (TCGA) transcriptomic dataset revealed overexpression of CDK1 in human colorectal cancers versus normal colon. (aacrjournals.org)
  • MCF-7 human breast cancer cells were used in this study. (ac.ir)
  • Apoptotic Killing of Breast Cancer Cells by IgYs Produced Against a Small 21 Aminoacid Epitope of the Human TRAIL-2 Receptor', Asian Pacific Journal of Cancer Prevention , 17(S3), pp. 293-297. (waocp.org)
  • The PCP4/PEP19 is a calmodulin-binding anti-apoptotic peptide in neural cells but its potential role in human cancer has largely been unknown. (oncotarget.com)
  • We investigated the expression of PCP4/PEP19 in human breast cancer cell lines MCF-7, SK-BR-3, and MDA-MB-231 cells, and found that estrogen receptor (ER)-positive MCF-7 and ER-negative SK-BR-3 cells expressed PCP4/PEP19. (oncotarget.com)
  • Cellular uptake of ginsenosides in Korean white ginseng and red ginseng and their apoptotic activities in human breast cancer cells. (qxmd.com)
  • Ginsenoside compound K, not Rb1, possesses potential chemopreventive activities in human colorectal cancer. (qxmd.com)
  • Global cancer transitions according to the Human Development Index (2008-2030): a population-based study. (koreascience.or.kr)
  • These studies support the contention that XIAP is a viable target for cancer therapy in human non-small cell lung cancer. (aacrjournals.org)
  • The altered ratio between bax and the antiapoptotic components Bcl-2 or Bcl-Xl, modulate the successive formation of the "apoptosoma" and the final apoptotic caspase cascade ( Green and Kroemer, 1998 ). (aspetjournals.org)
  • In further experiments, celecoxib was demonstrated to suppress apoptotic cell death elicited by anandamide, which is structurally similar to R(+)-MA. (aspetjournals.org)
  • We recently have reported the establishment and characterization of two human gastric scirrhous carcinoma cell lines, HSC-39 and HSC-43. (aacrjournals.org)
  • Kuribayashi N, Sakagami H, Iida M, Takeda M (1996) Chromatin structure and endonuclease sensitivity in human leukemic cell lines. (springer.com)
  • Action of recombinant human apoptotic endonuclease G on naked DNA and chromatin substrates: cooperation with exonuclease and DNase I. (mysciencework.com)
  • Here we report the action of the purified human recombinant form of this endonuclease on naked DNA and chromatin substrates. (mysciencework.com)
  • Our study provides the first evidence that α-bisabolol is a pro-apoptotic agent for primary human acute leukemia cells. (sigmaaldrich.com)
  • Here we test the pro-apoptotic potential of α-bisabolol against primary acute leukemia cells, including Philadelphia-negative and -positive B acute lymphoid leukemias (Ph - /Ph + B-ALL) and acute myeloid leukemias (AML), and against normal blood white cells and hematopoietic bone marrow stem cells. (biomedcentral.com)
  • This suppression was mediated by elevated apoptotic microparticles derived from dying cells during acute HIV-1 infection. (ox.ac.uk)
  • Exposure of phosphatidylserine on the apoptotic cell surface was partially responsible for complement activation and resulted in coating the apoptotic cell surface with C3bi. (rupress.org)
  • Complement activation is likely to be required for efficient uptake of apoptotic cells within the systemic circulation, and early component deficiencies could predispose to systemic autoimmunity by enhanced exposure to and/or aberrant deposition of apoptotic cells. (rupress.org)
  • These findings have renewed the interest on the concept that in genetically predisposed human subjects, prolonged exposure of β-cells to raised concentrations of circulating FFAs may contribute to qualitative and quantitative abnormalities of pancreatic islet function, leading to type 2 diabetes ( 7 ). (diabetesjournals.org)