The soft tissue filling the cavities of bones. Bone marrow exists in two types, yellow and red. Yellow marrow is found in the large cavities of large bones and consists mostly of fat cells and a few primitive blood cells. Red marrow is a hematopoietic tissue and is the site of production of erythrocytes and granular leukocytes. Bone marrow is made up of a framework of connective tissue containing branching fibers with the frame being filled with marrow cells.
Cells contained in the bone marrow including fat cells (see ADIPOCYTES); STROMAL CELLS; MEGAKARYOCYTES; and the immediate precursors of most blood cells.
The transference of BONE MARROW from one human or animal to another for a variety of purposes including HEMATOPOIETIC STEM CELL TRANSPLANTATION or MESENCHYMAL STEM CELL TRANSPLANTATION.
Removal of bone marrow and evaluation of its histologic picture.
Neoplasms located in the bone marrow. They are differentiated from neoplasms composed of bone marrow cells, such as MULTIPLE MYELOMA. Most bone marrow neoplasms are metastatic.
Progenitor cells from which all blood cells derive.
Techniques for the removal of subpopulations of cells (usually residual tumor cells) from the bone marrow ex vivo before it is infused. The purging is achieved by a variety of agents including pharmacologic agents, biophysical agents (laser photoirradiation or radioisotopes) and immunologic agents. Bone marrow purging is used in both autologous and allogeneic BONE MARROW TRANSPLANTATION.
The development and formation of various types of BLOOD CELLS. Hematopoiesis can take place in the BONE MARROW (medullary) or outside the bone marrow (HEMATOPOIESIS, EXTRAMEDULLARY).
A cytologic technique for measuring the functional capacity of stem cells by assaying their activity.
Transplantation between individuals of the same species. Usually refers to genetically disparate individuals in contradistinction to isogeneic transplantation for genetically identical individuals.
Progressive restriction of the developmental potential and increasing specialization of function that leads to the formation of specialized cells, tissues, and organs.
Irradiation of the whole body with ionizing or non-ionizing radiation. It is applicable to humans or animals but not to microorganisms.
Connective tissue cells of an organ found in the loose connective tissue. These are most often associated with the uterine mucosa and the ovary as well as the hematopoietic system and elsewhere.
A form of anemia in which the bone marrow fails to produce adequate numbers of peripheral blood elements.
Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others.
The clinical entity characterized by anorexia, diarrhea, loss of hair, leukopenia, thrombocytopenia, growth retardation, and eventual death brought about by the GRAFT VS HOST REACTION.
Transplantation of an individual's own tissue from one site to another site.
An organism whose body contains cell populations of different genotypes as a result of the TRANSPLANTATION of donor cells after sufficient ionizing radiation to destroy the mature recipient's cells which would otherwise reject the donor cells.
A progressive, malignant disease of the blood-forming organs, characterized by distorted proliferation and development of leukocytes and their precursors in the blood and bone marrow. Leukemias were originally termed acute or chronic based on life expectancy but now are classified according to cellular maturity. Acute leukemias consist of predominately immature cells; chronic leukemias are composed of more mature cells. (From The Merck Manual, 2006)
An encapsulated lymphatic organ through which venous blood filters.
Technique using an instrument system for making, processing, and displaying one or more measurements on individual cells obtained from a cell suspension. Cells are usually stained with one or more fluorescent dyes specific to cell components of interest, e.g., DNA, and fluorescence of each cell is measured as it rapidly transverses the excitation beam (laser or mercury arc lamp). Fluorescence provides a quantitative measure of various biochemical and biophysical properties of the cell, as well as a basis for cell sorting. Other measurable optical parameters include light absorption and light scattering, the latter being applicable to the measurement of cell size, shape, density, granularity, and stain uptake.
Leukocytes with abundant granules in the cytoplasm. They are divided into three groups according to the staining properties of the granules: neutrophilic, eosinophilic, and basophilic. Mature granulocytes are the NEUTROPHILS; EOSINOPHILS; and BASOPHILS.
Glycoproteins found on immature hematopoietic cells and endothelial cells. They are the only molecules to date whose expression within the blood system is restricted to a small number of progenitor cells in the bone marrow.
Elements of limited time intervals, contributing to particular results or situations.
Transfer of HEMATOPOIETIC STEM CELLS from BONE MARROW or BLOOD between individuals within the same species (TRANSPLANTATION, HOMOLOGOUS) or transfer within the same individual (TRANSPLANTATION, AUTOLOGOUS). Hematopoietic stem cell transplantation has been used as an alternative to BONE MARROW TRANSPLANTATION in the treatment of a variety of neoplasms.
Very large BONE MARROW CELLS which release mature BLOOD PLATELETS.
Clonal expansion of myeloid blasts in bone marrow, blood, and other tissue. Myeloid leukemias develop from changes in cells that normally produce NEUTROPHILS; BASOPHILS; EOSINOPHILS; and MONOCYTES.
An organism that, as a result of transplantation of donor tissue or cells, consists of two or more cell lines descended from at least two zygotes. This state may result in the induction of donor-specific TRANSPLANTATION TOLERANCE.
Deficiency of all three cell elements of the blood, erythrocytes, leukocytes and platelets.
Clonal hematopoietic stem cell disorders characterized by dysplasia in one or more hematopoietic cell lineages. They predominantly affect patients over 60, are considered preleukemic conditions, and have high probability of transformation into ACUTE MYELOID LEUKEMIA.
A malignancy of mature PLASMA CELLS engaging in monoclonal immunoglobulin production. It is characterized by hyperglobulinemia, excess Bence-Jones proteins (free monoclonal IMMUNOGLOBULIN LIGHT CHAINS) in the urine, skeletal destruction, bone pain, and fractures. Other features include ANEMIA; HYPERCALCEMIA; and RENAL INSUFFICIENCY.
Strains of mice in which certain GENES of their GENOMES have been disrupted, or "knocked-out". To produce knockouts, using RECOMBINANT DNA technology, the normal DNA sequence of the gene being studied is altered to prevent synthesis of a normal gene product. Cloned cells in which this DNA alteration is successful are then injected into mouse EMBRYOS to produce chimeric mice. The chimeric mice are then bred to yield a strain in which all the cells of the mouse contain the disrupted gene. Knockout mice are used as EXPERIMENTAL ANIMAL MODELS for diseases (DISEASE MODELS, ANIMAL) and to clarify the functions of the genes.
Lymphocytes responsible for cell-mediated immunity. Two types have been identified - cytotoxic (T-LYMPHOCYTES, CYTOTOXIC) and helper T-lymphocytes (T-LYMPHOCYTES, HELPER-INDUCER). They are formed when lymphocytes circulate through the THYMUS GLAND and differentiate to thymocytes. When exposed to an antigen, they divide rapidly and produce large numbers of new T cells sensitized to that antigen.
The survival of a graft in a host, the factors responsible for the survival and the changes occurring within the graft during growth in the host.
A glycoprotein of MW 25 kDa containing internal disulfide bonds. It induces the survival, proliferation, and differentiation of neutrophilic granulocyte precursor cells and functionally activates mature blood neutrophils. Among the family of colony-stimulating factors, G-CSF is the most potent inducer of terminal differentiation to granulocytes and macrophages of leukemic myeloid cell lines.
The production of red blood cells (ERYTHROCYTES). In humans, erythrocytes are produced by the YOLK SAC in the first trimester; by the liver in the second trimester; by the BONE MARROW in the third trimester and after birth. In normal individuals, the erythrocyte count in the peripheral blood remains relatively constant implying a balance between the rate of erythrocyte production and rate of destruction.
Process of classifying cells of the immune system based on structural and functional differences. The process is commonly used to analyze and sort T-lymphocytes into subsets based on CD antigens by the technique of flow cytometry.
Form of leukemia characterized by an uncontrolled proliferation of the myeloid lineage and their precursors (MYELOID PROGENITOR CELLS) in the bone marrow and other sites.
Relatively undifferentiated cells that retain the ability to divide and proliferate throughout postnatal life to provide progenitor cells that can differentiate into specialized cells.
Precursor of an alkylating nitrogen mustard antineoplastic and immunosuppressive agent that must be activated in the LIVER to form the active aldophosphamide. It has been used in the treatment of LYMPHOMA and LEUKEMIA. Its side effect, ALOPECIA, has been used for defleecing sheep. Cyclophosphamide may also cause sterility, birth defects, mutations, and cancer.
The number of WHITE BLOOD CELLS per unit volume in venous BLOOD. A differential leukocyte count measures the relative numbers of the different types of white cells.
Laboratory mice that have been produced from a genetically manipulated EGG or EMBRYO, MAMMALIAN.
The developmental history of specific differentiated cell types as traced back to the original STEM CELLS in the embryo.
The number of LEUKOCYTES and ERYTHROCYTES per unit volume in a sample of venous BLOOD. A complete blood count (CBC) also includes measurement of the HEMOGLOBIN; HEMATOCRIT; and ERYTHROCYTE INDICES.
Naturally occurring or experimentally induced animal diseases with pathological processes sufficiently similar to those of human diseases. They are used as study models for human diseases.
The number of CELLS of a specific kind, usually measured per unit volume or area of sample.
Differentiation antigens residing on mammalian leukocytes. CD stands for cluster of differentiation, which refers to groups of monoclonal antibodies that show similar reactivity with certain subpopulations of antigens of a particular lineage or differentiation stage. The subpopulations of antigens are also known by the same CD designation.
The fission of a CELL. It includes CYTOKINESIS, when the CYTOPLASM of a cell is divided, and CELL NUCLEUS DIVISION.
The cells found in the body fluid circulating throughout the CARDIOVASCULAR SYSTEM.
Individuals supplying living tissue, organs, cells, blood or blood components for transfer or transplantation to histocompatible recipients.
An alkylating agent having a selective immunosuppressive effect on BONE MARROW. It has been used in the palliative treatment of chronic myeloid leukemia (MYELOID LEUKEMIA, CHRONIC), but although symptomatic relief is provided, no permanent remission is brought about. According to the Fourth Annual Report on Carcinogens (NTP 85-002, 1985), busulfan is listed as a known carcinogen.
A single, unpaired primary lymphoid organ situated in the MEDIASTINUM, extending superiorly into the neck to the lower edge of the THYROID GLAND and inferiorly to the fourth costal cartilage. It is necessary for normal development of immunologic function early in life. By puberty, it begins to involute and much of the tissue is replaced by fat.
Antibodies produced by a single clone of cells.
Clonal hematopoetic disorder caused by an acquired genetic defect in PLURIPOTENT STEM CELLS. It starts in MYELOID CELLS of the bone marrow, invades the blood and then other organs. The condition progresses from a stable, more indolent, chronic phase (LEUKEMIA, MYELOID, CHRONIC PHASE) lasting up to 7 years, to an advanced phase composed of an accelerated phase (LEUKEMIA, MYELOID, ACCELERATED PHASE) and BLAST CRISIS.
A de novo myeloproliferation arising from an abnormal stem cell. It is characterized by the replacement of bone marrow by fibrous tissue, a process that is mediated by CYTOKINES arising from the abnormal clone.
Preparative treatment of transplant recipient with various conditioning regimens including radiation, immune sera, chemotherapy, and/or immunosuppressive agents, prior to transplantation. Transplantation conditioning is very common before bone marrow transplantation.
A neoplasm characterized by abnormalities of the lymphoid cell precursors leading to excessive lymphoblasts in the marrow and other organs. It is the most common cancer in children and accounts for the vast majority of all childhood leukemias.
The process of bone formation. Histogenesis of bone including ossification.
Identification of the major histocompatibility antigens of transplant DONORS and potential recipients, usually by serological tests. Donor and recipient pairs should be of identical ABO blood group, and in addition should be matched as closely as possible for HISTOCOMPATIBILITY ANTIGENS in order to minimize the likelihood of allograft rejection. (King, Dictionary of Genetics, 4th ed)
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
An individual that contains cell populations derived from different zygotes.
Genetically identical individuals developed from brother and sister matings which have been carried out for twenty or more generations, or by parent x offspring matings carried out with certain restrictions. All animals within an inbred strain trace back to a common ancestor in the twentieth generation.
The degree of antigenic similarity between the tissues of different individuals, which determines the acceptance or rejection of allografts.
Glycoproteins found in a subfraction of normal mammalian plasma and urine. They stimulate the proliferation of bone marrow cells in agar cultures and the formation of colonies of granulocytes and/or macrophages. The factors include INTERLEUKIN-3; (IL-3); GRANULOCYTE COLONY-STIMULATING FACTOR; (G-CSF); MACROPHAGE COLONY-STIMULATING FACTOR; (M-CSF); and GRANULOCYTE-MACROPHAGE COLONY-STIMULATING FACTOR; (GM-CSF).
In vitro method for producing large amounts of specific DNA or RNA fragments of defined length and sequence from small amounts of short oligonucleotide flanking sequences (primers). The essential steps include thermal denaturation of the double-stranded target molecules, annealing of the primers to their complementary sequences, and extension of the annealed primers by enzymatic synthesis with DNA polymerase. The reaction is efficient, specific, and extremely sensitive. Uses for the reaction include disease diagnosis, detection of difficult-to-isolate pathogens, mutation analysis, genetic testing, DNA sequencing, and analyzing evolutionary relationships.
The relatively long-lived phagocytic cell of mammalian tissues that are derived from blood MONOCYTES. Main types are PERITONEAL MACROPHAGES; ALVEOLAR MACROPHAGES; HISTIOCYTES; KUPFFER CELLS of the liver; and OSTEOCLASTS. They may further differentiate within chronic inflammatory lesions to EPITHELIOID CELLS or may fuse to form FOREIGN BODY GIANT CELLS or LANGHANS GIANT CELLS. (from The Dictionary of Cell Biology, Lackie and Dow, 3rd ed.)
The outward appearance of the individual. It is the product of interactions between genes, and between the GENOTYPE and the environment.
An acidic glycoprotein of MW 23 kDa with internal disulfide bonds. The protein is produced in response to a number of inflammatory mediators by mesenchymal cells present in the hemopoietic environment and at peripheral sites of inflammation. GM-CSF is able to stimulate the production of neutrophilic granulocytes, macrophages, and mixed granulocyte-macrophage colonies from bone marrow cells and can stimulate the formation of eosinophil colonies from fetal liver progenitor cells. GM-CSF can also stimulate some functional activities in mature granulocytes and macrophages.
Transplantation between genetically identical individuals, i.e., members of the same species with identical histocompatibility antigens, such as monozygotic twins, members of the same inbred strain, or members of a hybrid population produced by crossing certain inbred strains.
Proteins prepared by recombinant DNA technology.
The blood-making organs and tissues, principally the bone marrow and lymph nodes.
Disease having a short and relatively severe course.
RNA sequences that serve as templates for protein synthesis. Bacterial mRNAs are generally primary transcripts in that they do not require post-transcriptional processing. Eukaryotic mRNA is synthesized in the nucleus and must be exported to the cytoplasm for translation. Most eukaryotic mRNAs have a sequence of polyadenylic acid at the 3' end, referred to as the poly(A) tail. The function of this tail is not known for certain, but it may play a role in the export of mature mRNA from the nucleus as well as in helping stabilize some mRNA molecules by retarding their degradation in the cytoplasm.
A hematopoietic growth factor and the ligand of the cell surface c-kit protein (PROTO-ONCOGENE PROTEINS C-KIT). It is expressed during embryogenesis and is a growth factor for a number of cell types including the MAST CELLS and the MELANOCYTES in addition to the HEMATOPOIETIC STEM CELLS.
Bone-forming cells which secrete an EXTRACELLULAR MATRIX. HYDROXYAPATITE crystals are then deposited into the matrix to form bone.
A large multinuclear cell associated with the BONE RESORPTION. An odontoclast, also called cementoclast, is cytomorphologically the same as an osteoclast and is involved in CEMENTUM resorption.
A group of genetically identical cells all descended from a single common ancestral cell by mitosis in eukaryotes or by binary fission in prokaryotes. Clone cells also include populations of recombinant DNA molecules all carrying the same inserted sequence. (From King & Stansfield, Dictionary of Genetics, 4th ed)
Disorders of the blood and blood forming tissues.
Specialized forms of antibody-producing B-LYMPHOCYTES. They synthesize and secrete immunoglobulin. They are found only in lymphoid organs and at sites of immune responses and normally do not circulate in the blood or lymph. (Rosen et al., Dictionary of Immunology, 1989, p169 & Abbas et al., Cellular and Molecular Immunology, 2d ed, p20)
White blood cells formed in the body's lymphoid tissue. The nucleus is round or ovoid with coarse, irregularly clumped chromatin while the cytoplasm is typically pale blue with azurophilic (if any) granules. Most lymphocytes can be classified as either T or B (with subpopulations of each), or NATURAL KILLER CELLS.
Therapeutic act or process that initiates a response to a complete or partial remission level.
The return of a sign, symptom, or disease after a remission.
The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.
All of the processes involved in increasing CELL NUMBER including CELL DIVISION.
The restriction of a characteristic behavior, anatomical structure or physical system, such as immune response; metabolic response, or gene or gene variant to the members of one species. It refers to that property which differentiates one species from another but it is also used for phylogenetic levels higher or lower than the species.
Immunosuppression by reduction of circulating lymphocytes or by T-cell depletion of bone marrow. The former may be accomplished in vivo by thoracic duct drainage or administration of antilymphocyte serum. The latter is performed ex vivo on bone marrow before its transplantation.
The treatment of a disease or condition by several different means simultaneously or sequentially. Chemoimmunotherapy, RADIOIMMUNOTHERAPY, chemoradiotherapy, cryochemotherapy, and SALVAGE THERAPY are seen most frequently, but their combinations with each other and surgery are also used.
A specialized CONNECTIVE TISSUE that is the main constituent of the SKELETON. The principle cellular component of bone is comprised of OSTEOBLASTS; OSTEOCYTES; and OSTEOCLASTS, while FIBRILLAR COLLAGENS and hydroxyapatite crystals form the BONE MATRIX.
Evaluation undertaken to assess the results or consequences of management and procedures used in combating disease in order to determine the efficacy, effectiveness, safety, and practicability of these interventions in individual cases or series.
A general term for various neoplastic diseases of the lymphoid tissue.
Histochemical localization of immunoreactive substances using labeled antibodies as reagents.
Any of a group of malignant tumors of lymphoid tissue that differ from HODGKIN DISEASE, being more heterogeneous with respect to malignant cell lineage, clinical course, prognosis, and therapy. The only common feature among these tumors is the absence of giant REED-STERNBERG CELLS, a characteristic of Hodgkin's disease.
Mice homozygous for the mutant autosomal recessive gene "scid" which is located on the centromeric end of chromosome 16. These mice lack mature, functional lymphocytes and are thus highly susceptible to lethal opportunistic infections if not chronically treated with antibiotics. The lack of B- and T-cell immunity resembles severe combined immunodeficiency (SCID) syndrome in human infants. SCID mice are useful as animal models since they are receptive to implantation of a human immune system producing SCID-human (SCID-hu) hematochimeric mice.
A CXC chemokine that is chemotactic for T-LYMPHOCYTES and MONOCYTES. It has specificity for CXCR4 RECEPTORS. Two isoforms of CXCL12 are produced by alternative mRNA splicing.
The cells in the erythroid series derived from MYELOID PROGENITOR CELLS or from the bi-potential MEGAKARYOCYTE-ERYTHROID PROGENITOR CELLS which eventually give rise to mature RED BLOOD CELLS. The erythroid progenitor cells develop in two phases: erythroid burst-forming units (BFU-E) followed by erythroid colony-forming units (CFU-E); BFU-E differentiate into CFU-E on stimulation by ERYTHROPOIETIN, and then further differentiate into ERYTHROBLASTS when stimulated by other factors.
A variation of the PCR technique in which cDNA is made from RNA via reverse transcription. The resultant cDNA is then amplified using standard PCR protocols.
The movement of cells from one location to another. Distinguish from CYTOKINESIS which is the process of dividing the CYTOPLASM of a cell.
The release of stem cells from the bone marrow into the peripheral blood circulation for the purpose of leukapheresis, prior to stem cell transplantation. Hematopoietic growth factors or chemotherapeutic agents often are used to stimulate the mobilization.
Formation of LYMPHOCYTES and PLASMA CELLS from the lymphoid stem cells which develop from the pluripotent HEMATOPOIETIC STEM CELLS in the BONE MARROW. These lymphoid stem cells differentiate into T-LYMPHOCYTES; B-LYMPHOCYTES; PLASMA CELLS; or NK-cells (KILLER CELLS, NATURAL) depending on the organ or tissues (LYMPHOID TISSUE) to which they migrate.
Leukemia associated with HYPERPLASIA of the lymphoid tissues and increased numbers of circulating malignant LYMPHOCYTES and lymphoblasts.
Deliberate prevention or diminution of the host's immune response. It may be nonspecific as in the administration of immunosuppressive agents (drugs or radiation) or by lymphocyte depletion or may be specific as in desensitization or the simultaneous administration of antigen and immunosuppressive drugs.
The relationship between the dose of an administered drug and the response of the organism to the drug.
The span of viability of a cell characterized by the capacity to perform certain functions such as metabolism, growth, reproduction, some form of responsiveness, and adaptability.
A large lobed glandular organ in the abdomen of vertebrates that is responsible for detoxification, metabolism, synthesis and storage of various substances.
Neoplasms located in the blood and blood-forming tissue (the bone marrow and lymphatic tissue). The commonest forms are the various types of LEUKEMIA, of LYMPHOMA, and of the progressive, life-threatening forms of the MYELODYSPLASTIC SYNDROMES.
Established cell cultures that have the potential to propagate indefinitely.
Transfer of MESENCHYMAL STEM CELLS between individuals within the same species (TRANSPLANTATION, HOMOLOGOUS) or transfer within the same individual (TRANSPLANTATION, AUTOLOGOUS).
The domestic dog, Canis familiaris, comprising about 400 breeds, of the carnivore family CANIDAE. They are worldwide in distribution and live in association with people. (Walker's Mammals of the World, 5th ed, p1065)
An immunological attack mounted by a graft against the host because of tissue incompatibility when immunologically competent cells are transplanted to an immunologically incompetent host; the resulting clinical picture is that of GRAFT VS HOST DISEASE.
A protein-tyrosine kinase receptor that is specific for STEM CELL FACTOR. This interaction is crucial for the development of hematopoietic, gonadal, and pigment stem cells. Genetic mutations that disrupt the expression of PROTO-ONCOGENE PROTEINS C-KIT are associated with PIEBALDISM, while overexpression or constitutive activation of the c-kit protein-tyrosine kinase is associated with tumorigenesis.
Glycoprotein hormone, secreted chiefly by the KIDNEY in the adult and the LIVER in the FETUS, that acts on erythroid stem cells of the BONE MARROW to stimulate proliferation and differentiation.
A technique of culturing mixed cell types in vitro to allow their synergistic or antagonistic interactions, such as on CELL DIFFERENTIATION or APOPTOSIS. Coculture can be of different types of cells, tissues, or organs from normal or disease states.
High-molecular weight glycoproteins uniquely expressed on the surface of LEUKOCYTES and their hemopoietic progenitors. They contain a cytoplasmic protein tyrosine phosphatase activity which plays a role in intracellular signaling from the CELL SURFACE RECEPTORS. The CD45 antigens occur as multiple isoforms that result from alternative mRNA splicing and differential usage of three exons.
The phenotypic manifestation of a gene or genes by the processes of GENETIC TRANSCRIPTION and GENETIC TRANSLATION.
Non-antibody proteins secreted by inflammatory leukocytes and some non-leukocytic cells, that act as intercellular mediators. They differ from classical hormones in that they are produced by a number of tissue or cell types rather than by specialized glands. They generally act locally in a paracrine or autocrine rather than endocrine manner.
White blood cells. These include granular leukocytes (BASOPHILS; EOSINOPHILS; and NEUTROPHILS) as well as non-granular leukocytes (LYMPHOCYTES and MONOCYTES).
The number of PLATELETS per unit volume in a sample of venous BLOOD.
Any of the processes by which nuclear, cytoplasmic, or intercellular factors influence the differential control (induction or repression) of gene action at the level of transcription or translation.
A group of lymphocyte surface antigens located on mouse LYMPHOCYTES. Specific Ly antigens are useful markers for distinguishing subpopulations of lymphocytes.
Mice bearing mutant genes which are phenotypically expressed in the animals.
These growth factors comprise a family of hematopoietic regulators with biological specificities defined by their ability to support proliferation and differentiation of blood cells of different lineages. ERYTHROPOIETIN and the COLONY-STIMULATING FACTORS belong to this family. Some of these factors have been studied and used in the treatment of chemotherapy-induced neutropenia, myelodysplastic syndromes, and bone marrow failure syndromes.
A subnormal level of BLOOD PLATELETS.
The formation and development of blood cells outside the BONE MARROW, as in the SPLEEN; LIVER; or LYMPH NODES.
Family of RNA viruses that infects birds and mammals and encodes the enzyme reverse transcriptase. The family contains seven genera: DELTARETROVIRUS; LENTIVIRUS; RETROVIRUSES TYPE B, MAMMALIAN; ALPHARETROVIRUS; GAMMARETROVIRUS; RETROVIRUSES TYPE D; and SPUMAVIRUS. A key feature of retrovirus biology is the synthesis of a DNA copy of the genome which is integrated into cellular DNA. After integration it is sometimes not expressed but maintained in a latent state (PROVIRUSES).
A class of statistical procedures for estimating the survival function (function of time, starting with a population 100% well at a given time and providing the percentage of the population still well at later times). The survival analysis is then used for making inferences about the effects of treatments, prognostic factors, exposures, and other covariates on the function.
Red blood cells. Mature erythrocytes are non-nucleated, biconcave disks containing HEMOGLOBIN whose function is to transport OXYGEN.
A humoral factor that stimulates the production of thrombocytes (BLOOD PLATELETS). Thrombopoietin stimulates the proliferation of bone marrow MEGAKARYOCYTES and their release of blood platelets. The process is called THROMBOPOIESIS.
Immature, nucleated ERYTHROCYTES occupying the stage of ERYTHROPOIESIS that follows formation of ERYTHROID PRECURSOR CELLS and precedes formation of RETICULOCYTES. The normal series is called normoblasts. Cells called MEGALOBLASTS are a pathologic series of erythroblasts.
Antigens on surfaces of cells, including infectious or foreign cells or viruses. They are usually protein-containing groups on cell membranes or walls and may be isolated.
The use of two or more chemicals simultaneously or sequentially in the drug therapy of neoplasms. The drugs need not be in the same dosage form.
A pyrimidine nucleoside analog that is used mainly in the treatment of leukemia, especially acute non-lymphoblastic leukemia. Cytarabine is an antimetabolite antineoplastic agent that inhibits the synthesis of DNA. Its actions are specific for the S phase of the cell cycle. It also has antiviral and immunosuppressant properties. (From Martindale, The Extra Pharmacopoeia, 30th ed, p472)
Methods for maintaining or growing CELLS in vitro.
Blood of the fetus. Exchange of nutrients and waste between the fetal and maternal blood occurs via the PLACENTA. The cord blood is blood contained in the umbilical vessels (UMBILICAL CORD) at the time of delivery.
The transfer of STEM CELLS from one individual to another within the same species (TRANSPLANTATION, HOMOLOGOUS) or between species (XENOTRANSPLANTATION), or transfer within the same individual (TRANSPLANTATION, AUTOLOGOUS). The source and location of the stem cells determines their potency or pluripotency to differentiate into various cell types.
Granular leukocytes having a nucleus with three to five lobes connected by slender threads of chromatin, and cytoplasm containing fine inconspicuous granules and stainable by neutral dyes.
Stem cells derived from HEMATOPOIETIC STEM CELLS. Derived from these myeloid progenitor cells are the MEGAKARYOCYTES; ERYTHROID CELLS; MYELOID CELLS; and some DENDRITIC CELLS.
A prediction of the probable outcome of a disease based on a individual's condition and the usual course of the disease as seen in similar situations.
Agents that suppress immune function by one of several mechanisms of action. Classical cytotoxic immunosuppressants act by inhibiting DNA synthesis. Others may act through activation of T-CELLS or by inhibiting the activation of HELPER CELLS. While immunosuppression has been brought about in the past primarily to prevent rejection of transplanted organs, new applications involving mediation of the effects of INTERLEUKINS and other CYTOKINES are emerging.
Remnant of a tumor or cancer after primary, potentially curative therapy. (Dr. Daniel Masys, written communication)
Formation of MYELOID CELLS from the pluripotent HEMATOPOIETIC STEM CELLS in the BONE MARROW via MYELOID STEM CELLS. Myelopoiesis generally refers to the production of leukocytes in blood, such as MONOCYTES and GRANULOCYTES. This process also produces precursor cells for MACROPHAGE and DENDRITIC CELLS found in the lymphoid tissue.
The intracellular transfer of information (biological activation/inhibition) through a signal pathway. In each signal transduction system, an activation/inhibition signal from a biologically active molecule (hormone, neurotransmitter) is mediated via the coupling of a receptor/enzyme to a second messenger system or to an ion channel. Signal transduction plays an important role in activating cellular functions, cell differentiation, and cell proliferation. Examples of signal transduction systems are the GAMMA-AMINOBUTYRIC ACID-postsynaptic receptor-calcium ion channel system, the receptor-mediated T-cell activation pathway, and the receptor-mediated activation of phospholipases. Those coupled to membrane depolarization or intracellular release of calcium include the receptor-mediated activation of cytotoxic functions in granulocytes and the synaptic potentiation of protein kinase activation. Some signal transduction pathways may be part of larger signal transduction pathways; for example, protein kinase activation is part of the platelet activation signal pathway.
Removal and pathologic examination of specimens in the form of small pieces of tissue from the living body.
Conditions which cause proliferation of hemopoietically active tissue or of tissue which has embryonic hemopoietic potential. They all involve dysregulation of multipotent MYELOID PROGENITOR CELLS, most often caused by a mutation in the JAK2 PROTEIN TYROSINE KINASE.
The longest and largest bone of the skeleton, it is situated between the hip and the knee.
Group of rare congenital disorders characterized by impairment of both humoral and cell-mediated immunity, leukopenia, and low or absent antibody levels. It is inherited as an X-linked or autosomal recessive defect. Mutations occurring in many different genes cause human Severe Combined Immunodeficiency (SCID).
Adherence of cells to surfaces or to other cells.
Glycoproteins found on the membrane or surface of cells.
Antigens expressed primarily on the membranes of living cells during sequential stages of maturation and differentiation. As immunologic markers they have high organ and tissue specificity and are useful as probes in studies of normal cell development as well as neoplastic transformation.
Large, phagocytic mononuclear leukocytes produced in the vertebrate BONE MARROW and released into the BLOOD; contain a large, oval or somewhat indented nucleus surrounded by voluminous cytoplasm and numerous organelles.
Conditions in which the abnormalities in the peripheral blood or bone marrow represent the early manifestations of acute leukemia, but in which the changes are not of sufficient magnitude or specificity to permit a diagnosis of acute leukemia by the usual clinical criteria.
A severe sometimes chronic anemia, usually macrocytic in type, that does not respond to ordinary antianemic therapy.
Non-invasive method of demonstrating internal anatomy based on the principle that atomic nuclei in a strong magnetic field absorb pulses of radiofrequency energy and emit them as radiowaves which can be reconstructed into computerized images. The concept includes proton spin tomographic techniques.
Lymphocyte progenitor cells that are restricted in their differentiation potential to the B lymphocyte lineage. The pro-B cell stage of B lymphocyte development precedes the pre-B cell stage.
Mapping of the KARYOTYPE of a cell.
An immune response with both cellular and humoral components, directed against an allogeneic transplant, whose tissue antigens are not compatible with those of the recipient.
The process of generating white blood cells (LEUKOCYTES) from the pluripotent HEMATOPOIETIC STEM CELLS of the BONE MARROW. There are two significant pathways to generate various types of leukocytes: MYELOPOIESIS, in which leukocytes in the blood are derived from MYELOID STEM CELLS, and LYMPHOPOIESIS, in which leukocytes of the lymphatic system (LYMPHOCYTES) are generated from lymphoid stem cells.
Measurable and quantifiable biological parameters (e.g., specific enzyme concentration, specific hormone concentration, specific gene phenotype distribution in a population, presence of biological substances) which serve as indices for health- and physiology-related assessments, such as disease risk, psychiatric disorders, environmental exposure and its effects, disease diagnosis, metabolic processes, substance abuse, pregnancy, cell line development, epidemiologic studies, etc.
One of the mechanisms by which CELL DEATH occurs (compare with NECROSIS and AUTOPHAGOCYTOSIS). Apoptosis is the mechanism responsible for the physiological deletion of cells and appears to be intrinsically programmed. It is characterized by distinctive morphologic changes in the nucleus and cytoplasm, chromatin cleavage at regularly spaced sites, and the endonucleolytic cleavage of genomic DNA; (DNA FRAGMENTATION); at internucleosomal sites. This mode of cell death serves as a balance to mitosis in regulating the size of animal tissues and in mediating pathologic processes associated with tumor growth.
A group of differentiation surface antigens, among the first to be discovered on thymocytes and T-lymphocytes. Originally identified in the mouse, they are also found in other species including humans, and are expressed on brain neurons and other cells.
Morphologic alteration of small B LYMPHOCYTES or T LYMPHOCYTES in culture into large blast-like cells able to synthesize DNA and RNA and to divide mitotically. It is induced by INTERLEUKINS; MITOGENS such as PHYTOHEMAGGLUTININS, and by specific ANTIGENS. It may also occur in vivo as in GRAFT REJECTION.
CXCR receptors with specificity for CXCL12 CHEMOKINE. The receptors may play a role in HEMATOPOIESIS regulation and can also function as coreceptors for the HUMAN IMMUNODEFICIENCY VIRUS.
Studies used to test etiologic hypotheses in which inferences about an exposure to putative causal factors are derived from data relating to characteristics of persons under study or to events or experiences in their past. The essential feature is that some of the persons under study have the disease or outcome of interest and their characteristics are compared with those of unaffected persons.
The transfer of leukocytes from a donor to a recipient or reinfusion to the donor.
Antigens determined by leukocyte loci found on chromosome 6, the major histocompatibility loci in humans. They are polypeptides or glycoproteins found on most nucleated cells and platelets, determine tissue types for transplantation, and are associated with certain diseases.
The common chimpanzee, a species of the genus Pan, family HOMINIDAE. It lives in Africa, primarily in the tropical rainforests. There are a number of recognized subspecies.
Any detectable and heritable change in the genetic material that causes a change in the GENOTYPE and which is transmitted to daughter cells and to succeeding generations.
Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
Chronic refractory anemia with granulocytopenia, and/or thrombocytopenia. Myeloblasts and progranulocytes constitute 5 to 40 percent of the nucleated marrow cells.
The transfer of lymphocytes from a donor to a recipient or reinfusion to the donor.
A cytokine that stimulates the growth and differentiation of B-LYMPHOCYTES and is also a growth factor for HYBRIDOMAS and plasmacytomas. It is produced by many different cells including T-LYMPHOCYTES; MONOCYTES; and FIBROBLASTS.
An antineoplastic antimetabolite with immunosuppressant properties. It is an inhibitor of TETRAHYDROFOLATE DEHYDROGENASE and prevents the formation of tetrahydrofolate, necessary for synthesis of thymidylate, an essential component of DNA.
They are oval or bean shaped bodies (1 - 30 mm in diameter) located along the lymphatic system.
Serum containing GAMMA-GLOBULINS which are antibodies for lymphocyte ANTIGENS. It is used both as a test for HISTOCOMPATIBILITY and therapeutically in TRANSPLANTATION.
The physiological renewal, repair, or replacement of tissue.
Characteristic restricted to a particular organ of the body, such as a cell type, metabolic response or expression of a particular protein or antigen.
Substances that inhibit or prevent the proliferation of NEOPLASMS.
An alkylating nitrogen mustard that is used as an antineoplastic in the form of the levo isomer - MELPHALAN, the racemic mixture - MERPHALAN, and the dextro isomer - MEDPHALAN; toxic to bone marrow, but little vesicant action; potential carcinogen.
Bone marrow-derived lymphocytes that possess cytotoxic properties, classically directed against transformed and virus-infected cells. Unlike T CELLS; and B CELLS; NK CELLS are not antigen specific. The cytotoxicity of natural killer cells is determined by the collective signaling of an array of inhibitory and stimulatory CELL SURFACE RECEPTORS. A subset of T-LYMPHOCYTES referred to as NATURAL KILLER T CELLS shares some of the properties of this cell type.
Theoretical representations that simulate the behavior or activity of biological processes or diseases. For disease models in living animals, DISEASE MODELS, ANIMAL is available. Biological models include the use of mathematical equations, computers, and other electronic equipment.
Non-human animals, selected because of specific characteristics, for use in experimental research, teaching, or testing.
Congenital disorder affecting all bone marrow elements, resulting in ANEMIA; LEUKOPENIA; and THROMBOPENIA, and associated with cardiac, renal, and limb malformations as well as dermal pigmentary changes. Spontaneous CHROMOSOME BREAKAGE is a feature of this disease along with predisposition to LEUKEMIA. There are at least 7 complementation groups in Fanconi anemia: FANCA, FANCB, FANCC, FANCD1, FANCD2, FANCE, FANCF, FANCG, and FANCL. (from Online Mendelian Inheritance in Man,, August 20, 2004)
The classes of BONE MARROW-derived blood cells in the monocytic series (MONOCYTES and their precursors) and granulocytic series (GRANULOCYTES and their precursors).
A strain of non-obese diabetic mice developed in Japan that has been widely studied as a model for T-cell-dependent autoimmune insulin-dependent diabetes mellitus in which insulitis is a major histopathologic feature, and in which genetic susceptibility is strongly MHC-linked.
Leukemia induced experimentally in animals by exposure to leukemogenic agents, such as VIRUSES; RADIATION; or by TRANSPLANTATION of leukemic tissues.
Accumulation of a drug or chemical substance in various organs (including those not relevant to its pharmacologic or therapeutic action). This distribution depends on the blood flow or perfusion rate of the organ, the ability of the drug to penetrate organ membranes, tissue specificity, protein binding. The distribution is usually expressed as tissue to plasma ratios.
Protein analogs and derivatives of the Aequorea victoria green fluorescent protein that emit light (FLUORESCENCE) when excited with ULTRAVIOLET RAYS. They are used in REPORTER GENES in doing GENETIC TECHNIQUES. Numerous mutants have been made to emit other colors or be sensitive to pH.
A membrane-bound or cytosolic enzyme that catalyzes the synthesis of CYCLIC ADP-RIBOSE (cADPR) from nicotinamide adenine dinucleotide (NAD). This enzyme generally catalyzes the hydrolysis of cADPR to ADP-RIBOSE, as well, and sometimes the synthesis of cyclic ADP-ribose 2' phosphate (2'-P-cADPR) from NADP.
The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.
A bifunctional enzyme that catalyzes the synthesis and HYDROLYSIS of CYCLIC ADP-RIBOSE (cADPR) from NAD+ to ADP-RIBOSE. It is a cell surface molecule which is predominantly expressed on LYMPHOID CELLS and MYELOID CELLS.
The proportion of survivors in a group, e.g., of patients, studied and followed over a period, or the proportion of persons in a specified group alive at the beginning of a time interval who survive to the end of the interval. It is often studied using life table methods.
Specialized tissues that are components of the lymphatic system. They provide fixed locations within the body where a variety of LYMPHOCYTES can form, mature and multiply. The lymphoid tissues are connected by a network of LYMPHATIC VESSELS.
Death resulting from the presence of a disease in an individual, as shown by a single case report or a limited number of patients. This should be differentiated from DEATH, the physiological cessation of life and from MORTALITY, an epidemiological or statistical concept.
A classification of B-lymphocytes based on structurally or functionally different populations of cells.
Radiotherapy where cytotoxic radionuclides are linked to antibodies in order to deliver toxins directly to tumor targets. Therapy with targeted radiation rather than antibody-targeted toxins (IMMUNOTOXINS) has the advantage that adjacent tumor cells, which lack the appropriate antigenic determinants, can be destroyed by radiation cross-fire. Radioimmunotherapy is sometimes called targeted radiotherapy, but this latter term can also refer to radionuclides linked to non-immune molecules (see RADIOTHERAPY).
Trubowitz, S; Broers, A; Pease, R. F. (1970). "Surface ultrastructure of the human marrow--a brief note". Blood. 35 (1): 112-5 ...
There are three known accessible sources of autologous adult stem cells in humans: *Bone marrow, which requires extraction by ... The first human trial was approved by the US Food and Drug Administration in January 2009.[21] However, the human trial was not ... a b c d e Stem Cell Basics: What are the potential uses of human stem cells and the obstacles that must be overcome before ... "P-Selectin coated microtube for enrichment of CD34+ hematopoietic stem and progenitor cells from human bone marrow". Clinical ...
Austin, Shoshan Victoria (2012). "The True Human Body". In Carney, Eido Frances (ed.). Receiving the Marrow. Temple Ground ... as his successor as Guru instead of a possibly fallible human. Due to the faith's belief in complete equality, women can ...
Cell culture: Human or animal cell cultures are infected with the microbe of interest. These cultures are then observed to ... Møller M; El Maghrabi R; Olesen N; Thomsen VØ (November 2004). "Safe inoculation of blood and bone marrow for liquid culture ... Nester E; Anderson D; Evans Roberts, C; Nester M (2009). Microbiology: A human perspective. McGraw Hill. pp. 336-337. ISBN 1- ... their effects on the human body, and the methods of treating those infections. In this respect the entire field, as an applied ...
Cloning of human liver cDNA and a processed pseudogene on human chromosome 18q21.1". Drug Metab. Dispos. 23 (3): 398-405. PMID ... "The Mechanism and Drug Interaction - Allopurinol and Azathioprine and Risk of Bone Marrow Suppression". ... 1998). "Promoter and intronic sequences of the human thiopurine S-methyltransferase (TPMT) gene isolated from a human PAC1 ... "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.. .mw-parser-output ...
"Me too, too - How to make human embryonic stem cells without destroying human embryos". The Economist. 22 November 2007.. ... "Bone Marrow Research. 2012: 1-8. doi:10.1155/2012/787414. PMC 3398573. PMID 22830032. Archived from the original on 3 January ... Bone Marrow Transplant Retrieved on 21 November 2008 *^ Srivastava A, Bapat M, Ranade S, Srinivasan V, Murugan P, Manjunath S, ... MSCs have been isolated from placenta, adipose tissue, lung, bone marrow and blood, Wharton's jelly from the umbilical cord,[23 ...
Leukemia is a cancer of cells in the blood, and primarily affects the bone marrow where they are made. For most human leukemias ... Animal species other than humans[edit]. Leukaemias and lymphomas could potentially be monitored similarly in non-human animals ... The initial five weeks of treatment[clarification needed] kill most leukaemic cells, and the marrow begins to recover. Immature ... When a bone marrow sample is drawn, leukemic cells can be viewed under a microscope. Leukemic cells look like normal immature ...
Biol Blood Marrow Transplant". 17 (3), s. 401-11, Mar 2011. DOI: 10.1016/j.bbmt.2010.07.013. PMID: 20667478. ... Molecular genetics of human leukemias: new insights into therapy. „Semin Hematol". 39 (4 Suppl 3), s. 6-11, Oct 2002. DOI: ... Biol Blood Marrow Transplant". 17 (12), s. 1796-803, Dec 2011. DOI: 10.1016/j.bbmt.2011.06.005. PMID: 21699879. ... Human acute myeloid leukemia is organized as a hierarchy that originates from a primitive hematopoietic cell. „Nat Med". 3 (7 ...
When metastasis does occur, it is usually to the liver, spleen, lymph nodes and bone marrow. When mastocytomas affect humans, ... It is found in humans and many animal species; in human medicine it also can refer to an accumulation or nodule of mast cells ... or bone marrow, or with the presence of mast cells in the blood X-rays, ultrasound, or lymph node, bone marrow, or organ ... Mast cells originate from the bone marrow and are normally found throughout the connective tissue of the body as normal ...
Most human tissue and organ transplants are allografts. It is contrasted with autotransplantation (from one part of the body to ... An allogenic bone marrow transplant can result in an immune attack, called graft-versus-host disease. Informed consent is ... has regulated human tissue intended for transplants since 1993. In order to ensure the quality of donor tissue and reduce ... Allogeneic stem cell transplantation entry in the public domain NCI Dictionary of Cancer Terms Allogeneic bone marrow ...
Because primordial follicles can be dormant for up to 50 years in the human, the length of the ovarian cycle does not include ... Renewal of ovarian follicles from germline stem cells (originating from bone marrow and peripheral blood) was reported in the ... 2010). Human physiology : from cells to system. Australia ; United States: Brooks/Cole. ISBN 978-0-495-39184-5. Page 76 in: ... From birth, the ovaries of the human female contain a number of immature, primordial follicles. These follicles each contain a ...
... has been linked to an increase in bone marrow micronuclei in both mice and humans.[11] ... Human exposure[edit]. Cypermethrin is moderately toxic through skin contact or ingestion. It may cause irritation to the skin ... Human volunteers given dermal doses of 130 μg/cm2 on the earlobe experienced local tingling and burning sensations. One man ... Induction of micronuclei in mouse bone marrow by the insecticides cypermethrin and rotenone". Journal of Mutation Research. 155 ...
The Marrow of Human Experience: Essays on Folklore. University Press of Colorado. JSTOR j.ctt4cgkmk.. ... Josephson-Storm, Jason (2017). The Myth of Disenchantment: Magic, Modernity, and the Birth of the Human Sciences. University of ...
This dataset indicates that both in humans and mice, EPX is only expressed in the bone marrow. At this level, it is more than ... Human EPO is known to exist as a soluble monomer. The active site of eosinophil peroxidase contains a single iron atom in ... The open reading frame of human eosinophil peroxidase was found to have a length of 2,106 base pairs (bp). This comprises a 381 ... Eosinophils form part of the myelocytic lineage, one of two major classes of bone-marrow-derived cell types (along with the ...
The specificity of the human immune-cell repertoire is what allows the human body to defend itself from rapidly adapting ... derived from the bone marrow of adult donors. MSCs are purified from the marrow, cultured and packaged, with up to 10,000 doses ... as well as humans.[76][77][78][79] While investigation of cell-based therapeutics generally reflects human medical needs, the ... Fully mature human red blood cells may be generated ex vivo by hematopoietic stem cells (HSCs), which are precursors of red ...
Red blood cells are much smaller than most other human cells. RBCs are formed in the red bone marrow from hematopoietic stem ... Mature red blood cells are unique among cells in the human body in that they lack a nucleus (although erythroblasts do have a ... They are produced and derived from multipotent cells in the bone marrow known as a hematopoietic stem cells. Leukocytes are ... There are a variety of types of white bloods cells that serve specific roles in the human immune system. WBCs constitute ...
1977). "Human beta-globin messenger RNA. I. Nucleotide sequences derived from complementary RNA". J. Biol. Chem. 252 (14): 5019 ... The gamma globin genes (HBG1 and HBG2) are normally expressed in the fetal liver, spleen and bone marrow. Two gamma chains ... Hemoglobin subunit gamma-1 is a protein that in humans is encoded by the HBG1 gene.[3] ... Frier JA, Perutz MF (1977). "Structure of human foetal deoxyhaemoglobin". J. Mol. Biol. 112 (1): 97-112. doi:10.1016/S0022-2836 ...
P-Selectin coated microtube for enrichment of CD34+ hematopoietic stem and progenitor cells from human bone marrow". Clin Chem ... Bone Marrow Transplant". *↑ Kane, Ed. "Stem-cell therapy shows promise for horse soft-tissue injury ... "Making human embryonic stem cells", The Economist, 2007-11-22. *↑ Brand, Madeleine; Palca, Joe; Cohen, Alex (2007-11-20). „Skin ... Culture of Human Embryonic Stem Cells (hESC)". National Institutes of Health. Suarchyvuotas originalas 2010-01-06. Nuoroda ...
"Biology of Blood and Marrow Transplantation. 15 (12): 1628-33. PMC 2861656 . PMID 19896087. doi:10.1016/j.bbmt.2009.07.004.. ... Morgan S, Anderson RA, Gourley C, Wallace WH, Spears N (2012). "How do chemotherapeutic agents damage the ovary?". Human ... Krumbhaar EB (1919). "Role of the blood and the bone marrow in certain forms of gas poisoning". JAMA. 72: 39-41. doi:10.1001/ ... In very severe myelosuppression, which occurs in some regimens, almost all the bone marrow stem cells (cells that produce white ...
Signs of progressive marrow failure may warrant bone marrow transplantation (BMT). This has been used successfully to treat ... "American Journal of Human Genetics. 68 (4): 1048-54. doi:10.1086/319505. PMC 1275624. PMID 11254457.. ... Bone marrow is typically hypocellular, with maturation arrest in the myeloid lineages that give rise to neutrophils, ... The atomic structure of an archaeal ortholog of the human protein has been determined by x-ray crystallography and indicates a ...
Human brucellosis is usually not transmitted from human to human; people become infected by contact with fluids from infected ... Brucella is isolated from a blood culture on Castaneda medium or from bone marrow. Prolonged incubation (up to six weeks) may ... Transmission from human to human, for example through sexual intercourse or from mother to child, is exceedingly rare, but ... As of August 2013, Allison Rice-Ficht, Ph.D. at Texas A&M University and her team claim to be close to creating a human vaccine ...
"Bone Marrow Transplantation. 31: 411-412. doi:10.1038/sj.bmt.1703857.. Pemeliharaan CS1: Banyak nama: authors list (link) ... "Myco-protein from Fusarium venenatum: a well-established product for human consumption" (PDF). Journal Applied Microbiology ...
The human gene for MBP is on chromosome 18; the protein localizes to the CNS and to various cells of the hematopoietic system. ... However, MBP-related transcripts are also present in the bone marrow and the immune system. These mRNAs arise from the long MBP ... 1993). "The human myelin basic protein gene is included within a 179-kilobase transcription unit: expression in the immune and ... 1987). "The human myelin-basic-protein gene: chromosomal localization and RFLP analysis". Am. J. Hum. Genet. 40 (4): 365-73. ...
According to ayurveda, the human body is composed of tissues (dhatus), waste (malas), and biomaterials (doshas).[38] The seven ... dhatus are plasma (rasa), blood (rakta), muscles (māmsa), fat (meda), bone (asthi), marrow (majja), and semen (shukra). Like ... Hoernle, A. F. Rudolf (1907). Osteology or the bones of the human body. Oxford: Clarendon P. OCLC 28563136.. ... Patwardhan, Kishore (2008). Pabitra Kumar Roy (ed.). Concepts of Human Physiology in Ayurveda (PDF). Sowarigpa and Ayurveda. ...
"FALL-39, a putative human peptide antibiotic, is cysteine-free and expressed in bone marrow and testis". Proceedings of the ... "Cell differentiation is a key determinant of cathelicidin LL-37/human cationic antimicrobial protein 18 expression by human ... Gudmundsson GH, Agerberth B, Odeberg J, Bergman T, Olsson B, Salcedo R (June 1996). "The human gene FALL39 and processing of ... Higher plasma levels of human cathelicidin antimicrobial protein (hCAP18), which are up-regulated by vitamin D, appear to ...
"Human malignant osteopetrosis: pathophysiology, management and the role of bone marrow transplantation". Pediatr Transplant 3 ... July 2000). "Defects in TCIRG1 subunit of the vacuolar proton pump are responsible for a subset of human autosomal recessive ... "The mutational spectrum of human malignant autosomal recessive osteopetrosis". Hum. Mol. Genet. 10 (17): 1767-73. PMID ...
In 1999, he received the Eleanor Roosevelt Award for Human Rights. Marshall died June 2, 2003, aged 80, at his home in Newtown ... Connecticut, of complications of myelodysplasia, a bone marrow disorder. He was survived by his wife Violet P. Marshall, three ...
2008). "Hepatic differentiation of human bone marrow-derived mesenchymal stem cells by tetracycline-regulated hepatocyte ... This article on a gene on human chromosome 20 is a stub. You can help Wikipedia by expanding it.. *v ... Mincheva A, Lichter P, Schütz G, Kaestner KH (February 1997). "Assignment of the human genes for hepatocyte nuclear factor 3- ... is a protein that in humans is encoded by the FOXA2 gene.[5][6][7] ...
P-Selectin coated microtube for enrichment of CD34+ hematopoietic stem and progenitor cells from human bone marrow". Clin Chem ... Making human embryonic stem cells". The Economist. 2007-11-22.. *↑ Brand, Madeleine; Palca, Joe; Cohen, Alex (2007-11-20). „ ... Bone Marrow Transplant". Suarchyvuotas originalas 2009-11-04. Nuoroda tikrinta 2017-04-02.. ... Madhusankar N. „Use of Bone Marrow derived Stem Cells in Patients with Cardiovascular Disorders". Journal of Stem Cells and ...
2001). "Human alpha spectrin II and the FANCA, FANCC, and FANCG proteins bind to DNA containing psoralen interstrand cross- ... This phenotype is characterized by progressive bone marrow failure, cancer proneness and typical birth defects. The main ... Fanconi anemia group G protein is a protein that in humans is encoded by the FANCG gene. FANCG, involved in Fanconi anemia, ... "The human XRCC9 gene corrects chromosomal instability and mutagen sensitivities in CHO UV40 cells". Proc Natl Acad Sci U S A. ...
In humans, CD27 is a good marker for plasma cells, naive B cells are CD27-, memory B-cells are CD27+ and plasma cells are ... Recently they have been shown to reside for much longer periods in the bone marrow as long-lived plasma cells (LLPC). They ... Federico Caligaris-Cappio; Manlio Ferrarini (1997). Human B Cell Populations (Chemical Immunology) (v. 67). S. Karger AG ( ... After leaving the bone marrow, the B cell acts as an antigen presenting cell (APC) and internalizes offending antigens, which ...
MN1 is a gene found on human chromosome 22, with gene map locus 22q12.3-qter.[5] Its official full name is meningioma ( ... 2009). "Meningioma 1 gene is differentially expressed in CD34 positive cells from bone marrow of patients with myelodysplastic ... 2008). "Toward a confocal subcellular atlas of the human proteome". Mol. Cell. Proteomics. 7 (3): 499-508. doi:10.1074/mcp. ... "MN1, a novel player in human AML". Blood Cells Mol. Dis. 39 (3): 336-9. doi:10.1016/j.bcmd.2007.06.009. PMC 2387274. PMID ...
"Increased expression of preprotachykinin-I and neurokinin receptors in human breast cancer cells: implications for bone marrow ... One research team found a correlation in male fruit flies and discussed it as a possibility in other species, even humans.[35] ... Palma C, Maggi CA (2000). "The role of tachykinins via NK1 receptors in progression of human gliomas". Life Sciences. 67 (9): ... Gerard NP, Garraway LA, Eddy RL, Shows TB, Iijima H, Paquet JL, Gerard C (Nov 1991). "Human substance P receptor (NK-1): ...
The first physician to perform a successful human bone marrow transplant on a disease other than cancer was Robert A. Good at ... "Bone Marrow Transplant" redirects here. For the journal abbreviated Bone Marrow Transplant, see Bone Marrow Transplantation ( ... Bone marrow transplantation usually requires that the recipient's own bone marrow be destroyed (myeloablation). Prior to the ... Thomas' work showed that bone marrow cells infused intravenously could repopulate the bone marrow and produce new blood cells. ...
"P-Selectin coated microtube for enrichment of CD34+ hematopoietic stem and progenitor cells from human bone marrow". Clin Chem ... "Bone Marrow Res 2012: 787414. PMC 3398573. PMID 22830032. *↑ William JB; Prabakaran, Rajamanickam; Ayyappan, Subbu (2011). " ... "Making human embryonic stem cells". The Economist. 22.11.2007. *↑ Gahrton G, Björkstrand B (2000). "Progress in haematopoietic ... "US Department of Health and Human Services. 2004-07-14. *↑ 19,0 19,1 P. De Coppi, G Barstch, Anthony Atala (2007). "Isolation ...
List of human cell types derived from the germ layers. References[edit]. *^ .mw-parser-output cite.citation{font-style:inherit ... Osteochondroprogenitor cells are progenitor cells that arise from mesenchymal stem cells (MSC) in the bone marrow. They have ... before any genetic or morphological criteria were put in place for bone marrow or connective tissues. Osteoprogenitor cells can ...
The organization hosts events throughout the year to support the hematology/oncology/bone marrow transplant unit[71] at ... College of Education and Human Ecology College of Engineering College of Food, Agricultural, and Environmental Sciences ...
"Mapping the Ligand-binding Sites and Disease-associated Mutations on the Most Abundant Protein in the Human, Type I Collagen" ... liver, bone marrow, and lymphatic organs FunctionEdit. Connective tissue has a wide variety of functions that depend on the ...
Humans are born with four types of tonsils: the pharyngeal tonsil, two tubal tonsils, two palatine tonsils and the lingual ... which are two lymphoid organs situated at either side of the back of the human throat. The palatine tonsils and the adenoid ... "Evidence for a stepwise program of extrathymic T cell development within the human tonsil". Journal of Clinical Investigation ...
Voehringer, D.; M. Koschella; H. Pircher (2002). "Lack of proliferative capacity of human effector and memory T cells ... which is the organ essential for T-cell maturation following the migration of precursor cells from the bone marrow. This age- ... doi:10.1016/S0531-5565(99)00068-6. Franceschi, C.; M. Bonafè; S. Valensin (2000). "Human immunosenescence: the prevailing of ... "Age-related impairment of p56lck and ZAP-70 activities in human T lymphocytes activated through the TcR/CD3 complex". Exp ...
Gift of Life Marrow Registry. *Human Tissue Authority. *LifeSharers. *National Marrow Donor Program ... Hand transplantation is a surgical procedure to transplant a hand from one human to another. The "donor" hand usually comes ... The first short-term success in human hand transplant occurred with New Zealander Clint Hallam who had lost his hand in an ...
The human body's own cartilage is still the best material for lining knee joints. This drives efforts to develop ways of using ... is a biological treatment option for articular cartilage damage bone marrow stimulating technique in combination with a ... Recently, there have been several published case reports of successful cartilage growth in human knees using autologous ... using tissue engineering with a scaffold derived from a rat meniscus and mesenchymal stromal cells derived from rat bone marrow ...
... human leukocyte antigens (HLA) - human papilloma virus (HPV) - human T cell lymphotropic virus type I (HTLV-I) - human T cell ... bone marrow - bone marrow suppression - booster - branched DNA assay - breakthrough infection - Broadway Cares/Equity Fights ... human growth hormone (HGH) - human immunodeficiency virus type 1 (HIV-1) - human immunodeficiency virus type 2 (HIV-2) - ... Department of Health and Human Services (DHHS/HHS or DHHS) - desensitization - diabetes mellitus (DM) - diagnosis - diarrhea - ...
Marrow of Tragedy: The Health Crisis of the American Civil War (Johns Hopkins University Press; 2013) 400 pages; argues that ... Some viruses, like smallpox, have only human hosts and appeared to have never occurred on the North American continent before ... There was a fundamental difference in the human infectious diseases present in the indigenous peoples and that of sailors and ...
Blood cell dysfunction - megaloblastic anemia, myelodysplasia, marrow failure, marrow replacement, acute leukemia ... Maton D, Hopkins J, McLaughlin CW, Johnson S, Warner MQ, LaHart D, Wright JD, Kulkarni DV (1997). Human Biology and Health. ... increase of production in bone marrow, increased release from storage in bone marrow, decreased attachment to veins and ... All white blood cells are produced and derived from multipotent cells in the bone marrow known as hematopoietic stem cells. ...
"P-Selectin coated microtube for enrichment of CD34+ hematopoietic stem and progenitor cells from human bone marrow". Clin Chem ... "Transplantation of human embryonic stem cells onto a partially wounded human cornea in vitro". Acta Ophthalmologica 91 (2): 127 ... Bone Marrow Transplant». *↑ Ed Kane. «Stem-cell therapy shows promise for horse soft-tissue injury ... Making human embryonic stem cells». The Economist. 2007-11-22.. *↑ Ian Murnaghan for Explore Stem Cells. Updated: 16 December ...
In vitro, exposure of human bronchial epithelial cells or human pulmonary alveolar epithelial cells to agents such as hydrogen ... This agent also causes respiratory tract lesions, bone marrow depression, and eye damage, the epithelial tissues of these ... As a highly toxic and irritating material, it is hazardous to human health, and was involved in the Bhopal disaster-which ... 2011) Smad molecules expression pattern in human bronchial airway induced by sulfur mustard. Iran J Allergy Asthma Immunol. 10: ...
The human body depends on a vast array of biochemical reactions to support critical functions, including the production of ... For a long time, the most efficient approach had been to use bone marrow graft, or hematopoietic stem cell transplantation. ... Nearly every cell in the human body has 46 chromosomes, with 23 derived from each parent. The IDS gene is located on the X ... This is the first case of gene therapy being used in vivo in humans. Hurler syndrome Prenatal testing Genetic counseling Wraith ...
"Proof-of-concept study of biomarker development in mice provides a roadmap for a similar approach in humans". ... "Effect of homologous bone marrow injections in x-irradiated rabbits". British Journal of Experimental Pathology. 38 (4): 401- ... 2) The biomarker levels must be elevated in various forms and/or severities of human TBI in the acute phase (3-24 h post-injury ... Other biomarker candidates with biofluid evidence from animal models of TBI from severe to mTBI in human. These include ...
Human umbilical cord blood as a potential source of transplantable hematopoietic stem/progenitor cells. Proceedings of the ... KK Ballen, F Verter and J Kurtzberg Umbilical cord blood donation: public or private? Bone Marrow Transplantation (2015), 1-8 ... Increased migration of cord blood-derived CD34+ cells, as compared to bone marrow and mobilized peripheral blood CD34+ cells ...
They are usually the first cells to arrive at the site of an infection.[5] The bone marrow of a normal healthy adult produces ... A scanning electron microscope image of normal circulating human blood. One can see red blood cells, several knobby white blood ... They are produced by blood-forming stem cells in the bone marrow.[1] ...
মানব কঙ্কাল (Human skeleton). *অস্থি (Bone). *তরুণাস্থি (Cartilege). *অস্থিসন্ধি (Joint) *তন্তুময় অস্থিসন্ধি (Fibrous joint) ... অস্থি মজ্জা (Bone marrow). *থাইমাস গ্রন্থি (Thymus). *প্লীহা (Spleen). *তালুমূলগ্রন্থি (Tonsil). *লসিকাকোষ (Lymphocyte) ...
Campbell was the first to discover that cells of bone marrow origin contribute to intimal (the innermost coat of blood vessels ... This process is undergoing pre-clinical trials in humans and may be used to treat patients suffering coronary heart disease, ... Her most recent work involves the development of autologous vascular grafts from cells of bone marrow, known as the myeloid, ... she researched the biology of smooth muscle cells in normal artery walls of the human body. She recognized the importance of ...
Milligan DW; Howard MR; Judd A (1987). "Premedication with lorazepam before bone marrow biopsy". 》J Clin Pathol》 40 (6): 696-8 ... 1995). "Benzodiazepine receptors mediate regional blood flow changes in the living human brain". 》Proc. Natl. Acad. Sci. U.S.A ... Benzodiazepines: Effects on Human Performance and Behavior'(Central Police University Press, 2002). ... 1993). "[123I]iomazenil SPECT imaging demonstrates significant benzodiazepine receptor reserve in human and nonhuman primate ...
"100k Cheeks and Blood Center to Host Marrow Drive". February 1, 2011. Stanford Daily. Retrieved 16 June 2013. Aaker, Jennifer ... defined as the set of human characteristics associated with it. The five core dimensions are Sincerity, Excitement, Competence ... an organization dedicated to registering 100,000 South Asian donors in the National Bone Marrow Registry. In addition to ...
A scanning electron microscope image of normal circulating human blood. One can see red blood cells, several knobby white blood ... The bone marrow of a normal healthy adult produces more than 100 billion neutrophils per day, and more than 10 times that many ... Members of every class of pathogen that infect humans also infect plants. Although the exact pathogenic species vary with the ... Gong JH, Maki G, Klingemann HG (April 1994). "Characterization of a human cell line (NK-92) with phenotypical and functional ...
Over M (1992) (PDF). The macroeconomic impact of AIDS in Sub-Saharan Africa, Population and Human Resources Department. The ... "A Doctor, a Mutation and a Potential Cure for AIDS: A Bone Marrow Transplant to Treat a Leukemia Patient Also Gives Him Virus- ... Palefsky J (2007). "Human papillomavirus infection in HIV-infected persons". Top HIV Med 15 (4): 130-3. பப்மெட் 17720998. ... Klot, Jennifer; Monica Kathina Juma (2011). HIV/AIDS, Gender, Human Security and Violence in Southern Africa. Pretoria: Africa ...
T cells develop in the thymus, and B cells, in mammals, develop in the bone marrow in adults or the liver in fetuses." ... Willinger T, Freeman T, Hasegawa H, McMichael AJ, Callan MF (2005). „Molecular signatures distinguish human central memory from ... Generation of knock-in primary human T cells using Cas9 ribonucleoproteins". Proceedings of the National Academy of Sciences of ... Evidence for a stepwise program of extrathymic T cell development within the human tonsil". The Journal of Clinical ...
Researchers have developed an artificial tissue in which human blood stem cells remain functional for a prolonged period of ... Blood formation: Researchers engineer human bone marrow tissue. University of Basel. Journal. Proceedings of the National ... A new kind of artificial bone marrow. Now, researchers have engineered an artificial bone marrow niche, in which the stem and ... "We could use bone and bone marrow cells from patients to create an in vitro model of blood diseases such as leukemia, for ...
HBMF stands for Human Bone Marrow Fibroblast. HBMF is defined as Human Bone Marrow Fibroblast very rarely. ... *Chicago style: Acronym Finder. S.v. "HBMF." Retrieved June 16 ...,HBMF,/a,. ... ... Human Bone Marrow Endothelial Cell (hematology). *Human Brain ...
Viral infections caused by human adenovirus (HAdV) or CMV remain life-threatening complications in immunocompromised patients ... Detection of human cytomegalovirus-specific T lymphocytes in human blood: comparison of two methods. Cytotherapy 2008; 10: 834- ... Human AdV-specific T cells: persisting in vitro functionality despite lethal irradiation. *R Geyeregger. 1. , ... Bone Marrow Transplantation volume 49, pages934-941(2014)Cite this article ...
Human bone marrow has been used to create early-stage sperm cells for the first time, a scientific step forward that will help ... A new and alarming report finds that nearly 50 percent of men under age 60 may be infected with the human papillomavirus or HPV ...
655/500,000 CellsHuman Mesenchymal Stem Cells isolated from bone marrow and provided a low passage. These cells have the ... Category: Neuroscience Tags: human Mesenchymal Stem Cell Based Assays Human Mesenchymal Stem Cells Source Type: news ... Bone marrow mesenchymal stem cell-derived exosome uptake and retrograde transport can occur at peripheral nerve endings ... ...
TR expression and function in human bone marrow stromal and osteoblast-like cells.. Siddiqi A1, Parsons MP, Lewis JL, Monson JP ... in human bone marrow stromal cells (hBMS), which are believed to be the source of osteoblast progenitor cells, and for ... These results indicate that three main TR isoforms are expressed in cells of the human osteoblast lineage, but that expression ... but it is still not known whether TR isoform expression differs in primary cultures of human osteoblasts. We used ...
Primary CD105+ cells are isolated from bone marrow mononuclear cells using positive immunomagnetic separation techniques and ... human bone marrow cells,bone marrow CD105+ cells,bone marrow cd105+ cells,endothelial cells,endothelial cell,frozen bone marrow ... Primary human CD105+ cells were isolated from bone marrow (BM) mononuclear cells (MNCs) using positive immunomagnetic ... PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. ...
Primary stromal cells are derived from bone marrow mononuclear cells expanded in MesenCult™ or MesenCult™-ACF Culture Media and ... For Human Bone Marrow Stromal Cells (Catalog #70022) MNCs were cultured in MesenCult™ Proliferation Kit (Human; Catalog #05411) ... Internal Search Keywords: human bone marrow cells,stromal cells,bone marrow stromal cells,mesenchymal stem cells,mesenchymal ... Human Bone Marrow Stromal Cells Derived in ACF Medium, Frozen. Primary human cells, frozen ...
However, the impact of aging on the production of survival factors for effector/memory T cells and plasma cells in the human BM ... The bone marrow (BM) plays a key role in the long-term maintenance of immunological memory. ... "Inflamm-aging" influences immune cell survival factors in human bone marrow Eur J Immunol. 2017 Mar;47(3):481-492. doi: 10.1002 ... The bone marrow (BM) plays a key role in the long-term maintenance of immunological memory. However, the impact of aging on the ...
The chief executive of the Bone Marrow Donor Programme (BMDP) is out to bust myths about marrow donation and get more ... Human statues make stand on bone marrow donation. September 20, 2015ByTiffany Fumiko TayThe Straits Times. ... Of the 38 bone marrow transplants in Singapore this year, only five used bone marrow donated by locals. The rest came from ... The chief executive of the Bone Marrow Donor Programme (BMDP) is out to bust myths about marrow donation and get more ...
... that copurifies with mesenchymal stem cells from postnatal human bone marrow (BM) is a progenitor for angioblasts. In vitr … ... Origin of endothelial progenitors in human postnatal bone marrow J Clin Invest. 2002 Feb;109(3):337-46. doi: 10.1172/JCI14327. ... that copurifies with mesenchymal stem cells from postnatal human bone marrow (BM) is a progenitor for angioblasts. In vitro, ...
Breaking news and analysis of the global biotechnology, pharmaceutical, medical device and medical technology sectors. In-depth coverage of innovation, business, financing, regulation, science, product development, clinical trials and more
MicroRNA-499a Human bone marrow-derived mesenchymal stem cells (hBM-MSCs) Cardiomyogenesis Lentiviral vectors ... MicroRNA-499a-5p Promotes Differentiation of Human Bone Marrow-Derived Mesenchymal Stem Cells to Cardiomyocytes. ... Cardiomyocyte-like differentiation of human bone marrow mesenchymal stem cells after exposure of 5-azacytidine in vitro. Shi ... Human BM-MSCs (hBM-MSCs) were transduced with lentiviral vectors encoding miR-499a-3p or miR-499a-5p and analyzed by ...
Human marrow stromal cell treatment provides long-lasting benefit after traumatic brain injury in rats.. Mahmood A1, Lu D, Qu C ... This study was designed to investigate the effects of human bone marrow stromal cell (hMSC) administration in rats for 3 months ... Human Marrow Stromal Cell Treatment Provides Long-Lasting Benefit After Traumatic Brain Injury In Rats ... Human Marrow Stromal Cell Treatment Provides Long-Lasting Benefit After Traumatic Brain Injury In Rats ...
... and clinical studies related to all aspects of bone marrow. As an interdisciplinary journal, Bone Marrow Research aims to ... Bone Marrow Research is a peer-reviewed, Open Access journal that publishes original research articles, review articles, ... High-Frequency Vibration Treatment of Human Bone Marrow Stromal Cells Increases Differentiation toward Bone Tissue. D. Prè,1,2 ... D. Prè, G. Ceccarelli, L. Visai, et al., "High-Frequency Vibration Treatment of Human Bone Marrow Stromal Cells Increases ...
A mouse model of SLE to examine the effects of human bone marrow-derived mesenchymal stem cell (hBM-MSC) therapy on follicular ... MSCs derived from human bone marrow prevented autoAb production, proteinuria and nephritis. In another study, mouse bone marrow ... Human bone marrow-derived mesenchymal stem cell treatment in a Lupus mouse model. By Jenny Morgenweck, Ph.D. ... Jeehee Youn of Hanyang University in Seoul, Korea set out to fully evaluate the therapeutic effects of human bone marrow- ...
... and of the Notch ligands Delta-1 and Jagged-1 in human bone marrow stromal cells and in human hematopoietic precursors (3, 6-8 ... I-Delta1ext-myc enhances engraftment of human cells in marrow of NOD/SCID β2m-/- or NOD/SCID mice. (a and b) We transplanted ... Delta-1 enhances marrow and thymus repopulating ability of human CD34+CD38- cord blood cells. Kohshi Ohishi,1 Barbara Varnum- ... Cells cultured with ligand led to a 13-fold increase in the percentage of human CD45+ cells in marrow at 3 weeks (P , 0.001), ...
Clonally expanded novel multipotent stem cells from human bone marrow regenerate myocardium after myocardial infarction. ... Clonally expanded novel multipotent stem cells from human bone marrow regenerate myocardium after myocardial infarction. ... Human umbilical vein ECs (lane 4) and immortalized cell lines with high telomere length, provided by the Roche Diagnostics Corp ... We have identified a subpopulation of stem cells within adult human BM, isolated at the single-cell level, that self-renew ...
... is regulated through close interactions with stromal cells and components of the intracellular matrix in the bone marrow (BM) ... Human fetal brain. BM B progenitor cells. BM stromal cells (BMS). Human fetal brain. ... Lefkovits IMethods for purification and growth of human B cell precursors in bone marrow stromal cell-dependent culturesThe ... Wnt expression and canonical Wnt signaling in human bone marrow B lymphopoiesis ...
Here, we investigated the roles of S1P and S1P receptors (S1PRs) in migration of human BMSCs. We found that S1P exerted a ... In conclusion, we reveal that the S1P/S1PRs signaling axis regulates the migration of human BMSCs via a dual-directional ... Thus, selective modulation of S1PRs activity on human BMSCs may provide an effective approach to immunotherapy or tissue ... we demonstrated that S1PR1 and S1PR3 are responsible for S1P-induced migration of human BMSCs. In contrast, S1PR2 mediates the ...
... have uncovered evidence of the storage and delayed consumption of animal bone marrow at Qesem Cave near Tel Aviv, the site of ... Study finds prehistoric humans ate bone marrow like canned soup 400,000 years ago. by Tel Aviv University ... Study finds prehistoric humans ate bone marrow like canned soup 400,000 years ago. ... Citation: Study finds prehistoric humans ate bone marrow like canned soup 400,000 years ago (2019, October 9) retrieved 18 ...
... all human marrow is red, as the need for new blood formation is high. Fat tissue gradually replaces the red marrow, which in ... of the total body mass of humans. The red marrow, which constitutes the hematopoietic component of bone marrow, is responsible ... genes with group enriched expression including bone marrow and iii) genes with enhanced expression in bone marrow. Human tissue ... The transcriptome analysis shows that 58% (n=11395) of all human proteins (n=19613) are expressed in the bone marrow and 302 of ...
Isolation and Cultures of Human MSCs. To isolate human MSCs, bone marrow aspirates were taken from the iliac crest of normal ... Expansion of Human Adult Stem Cells from Bone Marrow Stroma: Conditions that Maximize the Yields of Early Progenitors and ... BMP-6 enhances chondrogenesis in a subpopulation of human marrow stromal cells. Biochem Biophys Res Commun 2001; 284: 411-418. ... In vitro aging of human bone marrow derived stromal cells. Mech Ageing Dev 1981; 16: 81-89. *CrossRef , ...
Human MSCs were cultivated on differently coated silicon wafers for 48 h. Subsequently, the cell proliferation rates were ... An enhanced proliferation of the human mesenchymal stem cells (hMSCs) would be beneficial. The present study was aimed to ... Behavior of Human Bone Marrow-Derived Mesenchymal Stem Cells on Various Titanium-Based Coatings Chengjuan Qu 1,2,*, Salla ... "Behavior of Human Bone Marrow-Derived Mesenchymal Stem Cells on Various Titanium-Based Coatings." Materials 9, no. 10: 827. ...
Allogeneic Human Bone Marrow Derived Mesenchymal Stem Cells in Localized Prostate Cancer (MSC). The safety and scientific ...
Human bone marrow stromal cells (hBMSCs) are adherent fibroblast-like cells found in the bone marrow. They are a heterogeneous ... 1135 Characterization of Human Bone Marrow Stromal Cells During Multi-passaging Friday, March 23, 2012: 3:30 p.m. - 4:45 p.m. ... and gene expression was examined with human Mesenchymal Stem Cell PCR Array (SABiosciences). Result: The proliferation rate of ...
Keywords: human bone marrow MSCs, cell sheets, microRNA, chitosan, nanoparticles, osteogenic differentiation ... and developing human bone marrow mesenchymal stem cell (hBMMSC) sheets with high osteogenic ability is a constant requirement ... Improving the osteogenesis of human bone marrow mesenchymal stem cell sheets by microRNA-21-loaded chitosan/hyaluronic acid ... Abbreviations: hBMMSC, human bone marrow mesenchymal stem cell; ALP, alkaline phosphatase; CS, chitosan; D, day; HA, hyaluronic ...
Scientists grow sperm from human bone marrow. Katy Sinclair. Progress Educational Trust. 17 April 2007. ... 1. Human eggs grown in a dish for the first time. 2. MSc in Clinical Embryology - Applications for entry in October 2018. 3. ... Scientists have claimed success in growing immature sperm cells from bone marrow, which they hope to employ in fertility ... took stem cell samples from the bone marrow of male volunteers. Usually the samples would be developed into cells that form ...
... induction in human lymphocytes and (2) in vivo micronucleus induction on rat bone marrow polychromatic erythrocytes (PCEs). ... Humans. Lymphocytes / drug effects*. Male. Micronucleus Tests. Mutagens / toxicity*. Rats. Rats, Wistar. Semicarbazides / ... Bone Marrow Cells / drug effects*. Cells, Cultured. Chromosomes / drug effects, ultrastructure. Cyclophosphamide / toxicity. ... Next Document: Effects of carbonyl cyanide p-(trifluoromethoxy) phenylhydrazone on growth inhibition in human pulmo.... ...
Here we demonstrate that 2D MSC expansion can be entirely bypassed by culturing freshly isolated bone marrow nucleated cells ...
  • Pure and Potent-Only $655/500,000 CellsHuman Mesenchymal Stem Cells isolated from bone marrow and provided a low passage. (
  • Primary human CD105+ cells were isolated from bone marrow (BM) mononuclear cells (MNCs) using positive immunomagnetic separation techniques. (
  • Immunoregulatory effects on T lymphocytes by human mesenchymal stromal cells isolated from bone marrow, amniotic fluid, and placenta. (
  • Now, researchers have engineered an artificial bone marrow niche, in which the stem and progenitor cells are able to multiply for a period of several days. (
  • In this respect, the artificial tissue had a very similar molecular structure to natural bone marrow niches, creating an environment in which the functionality of hematopoietic stem and progenitor cells could largely be maintained. (
  • We used immunocytochemistry, Western blotting, nuclear binding assays, and transient transfection studies to examine the expression of functional TR isoforms in primary cultures of osteoblasts (hOb) derived from explants of trabecular bone, in human bone marrow stromal cells (hBMS), which are believed to be the source of osteoblast progenitor cells, and for comparison in the transformed human osteosarcoma cell lines MG63 and SaOs-2. (
  • Human primary stromal cells (mesenchymal stem and progenitor cells) are produced by expanding bone marrow mononuclear cells (MNCs) in culture and cryopreserved following the first passage in culture. (
  • This study demonstrates that a CD34(-), vascular endothelial cadherin(-) (VE-cadherin(-)), AC133(+), and fetal liver kinase(+) (Flk1(+)) multipotent adult progenitor cell (MAPC) that copurifies with mesenchymal stem cells from postnatal human bone marrow (BM) is a progenitor for angioblasts. (
  • The results indicate that, when preparing cultures of human MSCs, it is necessary to compromise between conditions that provide the highest overall yields and those that provide the highest content of early progenitor cells. (
  • Since stem/progenitor cells recruited either from bone marrow or residing in nearby tissues can contribute to pathological processes we investigated endosialin in MSC using a novel monoclonal antibody. (
  • In this report we show that a clinic-ready an anti-human CD117 antibody called AMG 191 has significant activity against human and non-human primate (NHP) blood stem and progenitor cells. (
  • AMG 191 transiently and potently depletes the blood stem and progenitor cells in the bone marrow of NHP, and facilitates replacement by donor stem cells in mice that have been transplanted with human blood stem cells. (
  • Mobilization of hematopoietic stem/progenitor cells (HSPC) from the bone marrow (BM) is impaired in diabetes. (
  • Studies performed in experimental and human diabetes have consistently shown an impaired mobilization of hematopoietic stem/progenitor cells (HSPC) from the bone marrow (BM) to peripheral blood (PB) after stimulation with granulocyte-colony stimulation factor (G-CSF) ( 1 ). (
  • Radiation damage to the bone marrow compartment results in a dose-dependent acute depletion of stem cells, progenitor cells, and precursor cells of all cell lineages, as well as genotoxicity for cells surviving the direct cytotoxic effect [ 14 - 16 ]. (
  • We report the isolation of a cDNA clone that encodes a novel transmembrane isoform of the mucin-like glycoprotein MGC-24, expressed by both hematopoietic progenitor cells and elements of the bone marrow (BM) stroma. (
  • Of these, cell adhesion molecules (CAMs) play a major role in mediating interactions between primitive hematopoietic progenitor cells (HPCs) and various components of the bone marrow (BM) stroma. (
  • We develop a splenectomized xenograft model to demonstrate that transplantation of human primary AML into immunocompromised mice recapitulates the human disease course by induction of BM failure via depletion of mouse hematopoietic stem and progenitor populations. (
  • A 3D culture system enhances the ability of human bone marrow stromal cells to support the growth of limbal stem/progenitor cells. (
  • Coexpression of sialylated glycoproteins and glycolipids on bone-metastatic prostate tumor cells triggers robust E-selectin binding activity, which is identical to that observed on human hematopoietic progenitor cells. (
  • The lncRNA transcriptome in human hematopoietic stem and progenitor cells is not comprehensively defined. (
  • Our study provides a global view of lncRNAs in human hematopoietic stem and progenitor cells. (
  • Induction of apoptosis by benzene metabolites in HL60 and CD34+ human bone marrow progenitor cells. (
  • Two cell types, HL60 human promyelocytic leukemia cells and CD34+ human bone marrow progenitor cells, were used as model systems to explore a possible role for apoptosis in the myelotoxicity of the phenolic metabolites of benzene. (
  • These data show that apoptosis can be induced in both HL60 and CD34+ human bone marrow progenitor cells by benzene metabolites. (
  • The ability of phenolic metabolites of benzene to induce apoptosis in human bone marrow progenitor cells may contribute to benzene myelotoxicity. (
  • Identification and isolation of small CD44-negative mesenchymal stem/progenitor cells from human bone marrow using elutriation and polychromatic flow cytometry. (
  • We previously demonstrated that 3'-azido-3'-deoxythymidine (AZT) inhibits growth proliferation of human bone marrow progenitor cells in vitro [Antimicrob. (
  • Bone marrow-derived mesenchymal stem cells (MSCs) and cardiac progenitor cells (CPCs) have been used successfully as a cell-based approach for cardiac regeneration after myocardial infarction. (
  • Bone marrow-derived mesenchymal stem cells (BM-MSCs) are capable of self-renewal and differentiation into cardiomyocytes, albeit inefficiently. (
  • Human BM-MSCs (hBM-MSCs) were transduced with lentiviral vectors encoding miR-499a-3p or miR-499a-5p and analyzed by immunostaining and western blotting methods 14 days post-transduction. (
  • Bone marrow mesenchymal stem cells (BM-MSCs) improve heart function in swine myocardial infarction model through paracrine effects. (
  • MSCs derived from bone marrow can differentiate into many cells types, including osteoblasts, chrondrocytes, adipocytes and myoblasts. (
  • In SLE patient studies, MSCs derived from human bone marrow prevented autoAb production, proteinuria and nephritis. (
  • In another study, mouse bone marrow-derived MSCs attenuated nephritis. (
  • There is considerable interest in the biology and therapeutic potential of adult stem cells from bone marrow stroma, variously referred to as mesenchymal stem cells or marrow stromal cells (MSCs). (
  • Human MSCs can expand rapidly in culture, but the rate of expansion and the yields of multipotential progenitors are inversely related to the plating density and incubation time of each passage. (
  • One strategy for cell and gene therapy is to use adult stem cells from bone marrow stroma [ 1 - 4 ], referred to as mesenchymal stem cells or marrow stromal cells (MSCs). (
  • Human MSCs were cultivated on differently coated silicon wafers for 48 h. (
  • Fibrin and alginate hydrogels have been widely used to support chondrogenesis of bone marrow-derived mesenchymal stem cells (BM-MSCs) for articular cartilage and fibrocartilage tissue engineering, with each material offering distinct advantages and disadvantages. (
  • Although bone marrow represents the main source of MSCs, there remains a need to identify a stem cell source that is safe and easily accessible and yields large numbers of cells without provoking debates over ethics. (
  • In this study, MSCs isolated from amniotic fluid and placenta were compared with bone marrow MSCs. (
  • We discovered a novel small-molecule modulator 5{i,2} that can specifically induce the chondrogenic differentiation of human bone marrow-derived mesenchymal stem cells (hBM-MSCs). (
  • Bone marrow-derived mesenchymal stromal cells (MSCs) are promising candidates for tissue engineering-based cell therapies due to their multipotent character. (
  • In this study, we show that the use of a biaxial rotating bioreactor, after static culture of human fetal MSCs (hfMSCs) seeded on synthetic polymeric scaffolds, improved the homogeneity of cell and extracellular matrix distribution and increased the total cell number. (
  • 11 Based on our previous findings on VSMC differentiation of human bone marrow MSCs, 12 we investigated activation and inhibition of the PI3K/Akt/mTOR/p70S6K pathway in the phenotypic modulation and expression of VSMC markers in MSCs. (
  • Human MSCs were isolated by plastic adherence from aspirated iliac crest marrow from consenting normal adult donors and cultured as described previously. (
  • Long-term preservation and easy transportation of human bone marrow-derived mesenchymal stem cells (hBM-MSCs) will facilitate their application in medical treatment and bioengineering. (
  • Human bone marrow-derived mesenchymal stem cells (hBM-MSCs) are multipotent stem cells that can proliferate, support the immune system, and differentiate into multiple lineages. (
  • Therefore, we investigated and compared cytokine and gene expression levels from adipose (AD) and bone marrow (BM)-derived MSCs relevant to immune modulation from early to late passages. (
  • However, the gene expressions of tumor necrosis factor-stimulated gene (TSG)-6 and human leukocyte antigen (HLA)-G were decreased and gene expressions of galectin-1 and -3 were increased in both AD- and BM-MSCs with repeated passages. (
  • The objective of this study was to prepare a cell-free decellularized human IVD scaffold and to compare neotissue formation in response to recellularization with human IVD cells (hIVDCs) or human bone marrow-derived (hBM) mesenchymal stromal cells (MSCs). (
  • Because of their easy accession, multipotency, and lower immunogenicity, bone marrow-derived mesenchymal stem cells (BM-MSCs) represent as an ideal biological source for cell therapy. (
  • After 1 h of NMP, 0, 107 cultured human adipose tissue-derived MSCs, or 107 cultured bone marrow-derived MSCs were added (n = 5 per group). (
  • Also, elevated levels of human hepatocyte growth factor, interleukin (IL)-6, and IL-8 were found in the perfusate of the groups perfused with MSCs compared to the control groups. (
  • Platelet products (PP) and bone-marrow aspirate are popular sources of osteoinductive signalling molecules and osteogenic bone marrow mesenchymal stromal cells (BM-MSCs) used in the treatment of impaired bone healing. (
  • This systematic review investigates the effects of PP on the biophysiological functions of BM-MSCs in in vitro human studies, including (i) proliferation, (ii) migration, (iii) differentiation, (iv) growth factor/cytokine/protein expression, (v) immunomodulation, (vi) chemotactic effect on haematopoietic stem cells, (vii) response to apoptotic stress, and (viii) gene expression. (
  • In vitro studies in human have demonstrated the multi-faceted 'priming effect' of PP on the biophysiological functions of BM-MSCs. (
  • Therefore, more collaborative in vitro research in humans modelled to reflect clinical practice is required to better understand the effects of PP exposure on the biophysiological function(s) of BM-MSCs in human. (
  • Human MSCs were co-cultured with neonatal rat ventricular cardiomyocytes (NRVMs). (
  • Furthermore, we replicated the cardiac stem cell niche by combining human CPCs and MSCs in an in vitro model (the same lentiviral constructs were used). (
  • In order to determine a possible relationship between the two granule subsets, immunoelectron-microscopic techniques were used to determine the presence and precise intragranular distribution of major basic protein (MBP), eosinophil cationic protein (ECP), eosinophil peroxidase (EPO), and arylsulfatase B of eosinophil granules, as well as the Charcot-Leyden crystal (CLC) protein, in eosinophil progenitors of the bone marrow. (
  • Consistent with this, we show evidence that bone marrow progenitors from healthy HCMV carriers contain endogenous HCMV DNA as detected by PCR. (
  • These observations are consistent with bone marrow progenitors acting as a reservoir for HCMV and transmitting the viral genome to PBM, in the absence of lytic-gene expression, until they leave the circulation and undergo tissue-specific differentiation to macrophages. (
  • Parallel in vitro studies of human B cell development were performed either using fetal hematopoietic sources ( 14 , 15 , 16 , 17 , 18 , 19 , 20 ) or using hybrid cocultures with human progenitors and murine stromal cell lines ( 21 , 22 , 23 ). (
  • Here we sought to compare the cardiac repair efficacy of three promising human cell types: bone marrow mononuclear cells (hBMMNC), human embryonic stem cell-derived cardiomyocytes (hESC-CM) and hESC-derived cardiovascular progenitors (hESC-CVP). (
  • 3 Animal and human studies have indicated that neointimal cells may not only originate from the vascular media or adventitia, but also from bone marrow- and nonmarrow-derived progenitors. (
  • Human CD34+ bone marrow progenitors were expanded in semi-solid cultures with serum-replete medium containing c-kit-ligand, GM-CSF, and TNF-alpha. (
  • We conclude that normal human CD34+ bone marrow cells include substantial numbers of clonogenic progenitors, distinct from CFU-GMs, that can give rise to pure dendritic cell colonies. (
  • Cumulatively, these studies show that the alternative splicing of multiple prosurvival BCL2 family genes promotes malignant transformation of myeloid progenitors into BC LSCS that are quiescent in the marrow niche and that contribute to therapeutic resistance. (
  • Notably, sabutoclax, a pan-BCL2 inhibitor, renders marrow-niche-resident BC LSCs sensitive to TKIs at doses that spare normal progenitors. (
  • To do this, they combined human mesenchymal stromal cells with a porous, bone-like 3D scaffold made of a ceramic material in what is known as a perfusion bioreactor, which was used to combine biological and synthetic materials. (
  • By implanting ceramic scaffolds coated with human mesenchymal stromal cells into immune-deficient mice, we were able to mimic the human bone marrow niche. (
  • Neuronal plasticity of human Wharton's jelly mesenchymal stromal cells to the dopaminergic cell type compared with human bone marrow mesenchymal st. (
  • Mesenchymal stromal cells (MSC) derived from Wharton's jelly (WJ) of the umbilical cord are increasingly gaining prominence as substitutes for bone marrow (BM) MSC. (
  • Researchers have developed an artificial tissue in which human blood stem cells remain functional for a prolonged period of time. (
  • Every day in the bone marrow several billion blood cells are formed. (
  • This constant supply is ensured by blood stem cells located in special niches within the marrow. (
  • These stem cells can multiply and mature into red and white blood cells, which then leave the bone marrow and enter the bloodstream. (
  • We could use bone and bone marrow cells from patients to create an in vitro model of blood diseases such as leukemia, for example. (
  • Importantly, we could do this in an environment that consists exclusively of human cells and which incorporates conditions tailored to the specific individual," explain Ivan Martin and Timm Schroeder. (
  • Novel very sensitive detection methods combined with our recently developed short-term expansion protocol 12 prompted us to test the effector functions of very rare human adenovirus (HAdV)-specific T cells after irradiation with 30 Gy, as performed for normal GCs. (
  • Human bone marrow has been used to create early-stage sperm cells for the first time, a scientific step forward that will help researchers understand more about how sperm cells are created. (
  • TR expression and function in human bone marrow stromal and osteoblast-like cells. (
  • TRs have been identified in cells of the osteoblast lineage, but it is still not known whether TR isoform expression differs in primary cultures of human osteoblasts. (
  • These results indicate that three main TR isoforms are expressed in cells of the human osteoblast lineage, but that expression and endogenous TR activity are predominantly present in hBMS cells. (
  • Human bone marrow stromal cells derived in ACF medium (Catalog #70071) using the MesenCult™-ACF Culture Kit (Catalog #05449) differentiate to A) adipocytes (Oil Red O staining), B) chondrocytes (Alcian Blue and Nuclear Fast Red staining) and C) osteoblasts (Alizarin Red S staining). (
  • However, the impact of aging on the production of survival factors for effector/memory T cells and plasma cells in the human BM has not been studied. (
  • Bone marrow mesenchymal stem cells: biological properties and their role in hematopoiesis and hematopoietic stem cell transplantation. (
  • 2016). Differentiation of bone marrow mesenchymal stem cells to cardiomyocyte-like cells is regulated by the combined low dose treatment of transforming growth factor-β1 and 5-azacytidine. (
  • Cardiomyocyte-like differentiation of human bone marrow mesenchymal stem cells after exposure of 5-azacytidine in vitro. (
  • Differentiation of bone marrow mesenchymal stem cells induced by myocardial medium under hypoxic conditions [Internet]. (
  • Bone marrow mesenchymal stem cells for post-myocardial infarction cardiac repair: microRNAs as novel regulators. (
  • High-Frequency Vibration Treatment of Human Bone Marrow Stromal Cells Increases Differentiation toward Bone Tissue," Bone Marrow Research , vol. 2013, Article ID 803450, 13 pages, 2013. (
  • We show that activation of endogenous Notch signaling in human CD34 + CD38 - cord blood precursors with immobilized Delta-1 in serum-free cultures containing fibronectin and hematopoietic growth factors inhibited myeloid differentiation and induced a 100-fold increase in the number of CD34 + cells compared with control cultures. (
  • Transplantation into immunodeficient mice showed a substantial increase in myeloid and B cell engraftment in the marrow and also revealed thymic repopulation by CD3 + T cells due to cells being cultured for a longer period with immobilized Delta-1. (
  • We have identified a subpopulation of stem cells within adult human BM, isolated at the single-cell level, that self-renew without loss of multipotency for more than 140 population doublings and exhibit the capacity for differentiation into cells of all 3 germ layers. (
  • Based on surface marker expression, these clonally expanded human BM-derived multipotent stem cells (hBMSCs) do not appear to belong to any previously described BM-derived stem cell population. (
  • To our knowledge, this is the first demonstration that a specific population of multipotent human BM-derived stem cells can induce both therapeutic neovascularization and endogenous and exogenous cardiomyogenesis. (
  • BACKGROUND: The early B lymphopoiesis in mammals is regulated through close interactions with stromal cells and components of the intracellular matrix in the bone marrow (BM) microenvironment. (
  • The recruitment of bone marrow-derived mesenchymal stem cells (BMSCs) to damaged tissues and sites of inflammation is an essential step for clinical therapy. (
  • An enhanced proliferation of the human mesenchymal stem cells (hMSCs) would be beneficial. (
  • Lammi, M.J. Behavior of Human Bone Marrow-Derived Mesenchymal Stem Cells on Various Titanium-Based Coatings. (
  • Human bone marrow stromal cells (hBMSCs) are adherent fibroblast-like cells found in the bone marrow. (
  • Cell sheet engineering has emerged as a novel approach to effectively deliver seeding cells for tissue regeneration, and developing human bone marrow mesenchymal stem cell (hBMMSC) sheets with high osteogenic ability is a constant requirement from clinics for faster and higher-quality bone formation. (
  • 2 , 3 However, the traditional transplanted models, such as direct cell suspension injection or combination with biomaterials, have tended to present low efficacy, as well as poor survival of the seeding cells, which might impede the expected therapeutic effects in humans. (
  • Scientists have claimed success in growing immature sperm cells from bone marrow, which they hope to employ in fertility treatments within five years. (
  • To begin to understand the mechanisms that regulate self-renewal, differentiation, and transformation of human hematopoietic stem cells or to evaluate the efficacy of novel treatment modalities, stem cells need to be studied in their own species-specific microenvironment. (
  • Despite a continuous improvement in xenograft models for studying human hematopoiesis in vivo, engraftment of human malignant cells remains challenging. (
  • Mesenchymal stem cells from human bone marrow (hMSC) have the potential to differentiate into hepatocyte-like cells in vitro and continue to maintain important hepatocyte functions in vivo after transplantation into host mouse livers. (
  • Clusters of transplanted cells appeared predominantly in the periportal portion of the liver lobule and secreted human albumin featuring a prominent quality of differentiated hepatocytes. (
  • Immunohistochemistry for human endosialin in xenograft tumors following co-injection of MSC and cancer cells identified MSC in tumor stroma. (
  • The present study investigates a new family of bioactive glass nanoparticles with and without Sr-doping focusing on the influence of the nanoparticles on human bone marrow stromal cells (hBMSCs) in vitro. (
  • Successful engraftment of blood forming stem cells into recipients requires overcoming barriers that hinder access of stem cells to specialized niches in bone marrow. (
  • We also show that bone marrow precursors are readily infected by clinical isolates of HCMV in vitro but that no viral gene expression occurs until these cells become differentiated. (
  • Human bone marrow established in the 3D bioreactor demonstrated an average percentage of RBCs among total viable cells peaking at 21% on day 21. (
  • ANN ARBOR, Mich. Artificial bone marrow that can continuously make red and white blood cells has been created in a University of Michigan lab. (
  • The scaffolds were then seeded with bone marrow stromal cells and osteoblasts, another type of bone marrow cell. (
  • Certain stem cells that are essential for immunity and blood production are able to grow, divide and differentiate efficiently in these scaffolds due to the close similarity of the pores in the scaffold and the pores in actual bone marrow. (
  • The mice produced human immune cells and blood vessels grew through the substance. (
  • Gremlin2 Suppression Increases the BMP-2-Induced Osteogenesis of Human Bone Marrow-Derived Mesenchymal Stem Cells Via the BMP-2/Smad/Runx2 Signaling Pathway. (
  • In this study we have analyzed the interaction between in vitro cultured bone marrow stromal cells (BMSC) and NK cells. (
  • It is generally accepted that human NK cells play a role in eliminating virus-infected cells as well as in controlling tumor cell growth ( 1 , 2 , 3 , 4 ). (
  • One challenge was the inability to differentiate human cord blood (CB) or adult bone marrow (BM) hematopoietic stem cells (HSCs) without murine stroma. (
  • Flt3 ligand, but not thymic stromal-derived lymhopoietin (TSLP), was required for the IL-7-independent production of human B lineage cells. (
  • In vivo human data from clinical reports indicated that patients having severe combined immunodeficiency due to defects in IL-7/IL-7R signaling lack T cells, but have peripheral blood B cells ( 24 , 25 , 26 ). (
  • Rats were randomly assigned to the following groups: hESC-CVP n=10, hESC-CM n=11, hBMMNC n=11 and non-cardiac cells (control) derived from human ESCs (hNC) n=13. (
  • Human cells were identified by in situ hybridation with a human pan centromeric probe and graft composition analyzed by immunohistochemistry. (
  • In contrast no (hBMMNC) or few (hNC) human cells were detected in other groups. (
  • The migration of bone marrow mesenchymal stem cells (BMSCs) into bone defect sites or material implantation sites, and their differentiation into osteoblasts, is central to the fracture healing process, and the directional migration of BMSCs depends on cytokines or chemokines at the defect site. (
  • developed a mouse model that mimics the course of human disease and determined that acute myeloid leukemia cells produce a cytokine called interleukin-6, which interferes with red blood cell differentiation. (
  • Because of their immunomodulatory properties, human bone marrow stromal cells (hBMSCs) represent promising stem cells for treatment of immune disorders. (
  • For the experiment, Prof Nayernia and his team took bone marrow from male volunteers and isolated the mesenchymal stem cells. (
  • Earlier research led by Prof Nayernia using mice, published in Laboratory Investigations, also created spermatagonial cells from mouse bone marrow. (
  • The human bone marrow stromal cells (HBMSC) described in this paper developed a calcified matrix, expressed osteocalcin (OC), osteopontin (OP) and responded to 1,25(OH) 2 D 3 . (
  • We investigated whether bone marrow stromal cells (BMSCs), also known as bone marrow-derived mesenchymal stem cells, could serve as feeder cells for the expansion of LSCs in the 3-dimensional (3D) system. (
  • To investigate whether human bone marrow (BM) derived mesenchymal stem cells (MSC) and articular chondrocytes (AC) affect the in vitro proliferation of T-lymphocytes and peripheral blood mononuclear cells (PBMC) driven by the homeostatic IL 2, IL 7 and IL 15 cytokines binding to the common cytokine receptor γ-chain (γc ) in the absence of T-cell receptor (TCR) triggering. (
  • Prostate tumor cells preferentially adhere to bone marrow endothelial cells (BMECs) compared with endothelial linings from other tissue microvessels, implicating the importance of BMEC adhesion in the predilection of prostate tumor metastasis to bone. (
  • In this report, we demonstrate for the first time that human bone-metastatic prostate tumor cells roll on human BMECs under physiological flow conditions. (
  • We report here the isolation of a population of non-transformed pluripotent human cells from bone marrow after a unique expansion/selection procedure. (
  • Marrow-adherent and -nonadherent cells were co-cultured on fibronectin, at low oxygen tension, for 14 days. (
  • Based on their unique characteristics and properties, we refer to them as human marrow-isolated adult multilineage inducible cells, or MIAMI cells. (
  • We have isolated a unique subpopulation of human marrow stromal cells capable of differentiating in vitro into cell lineages derived from all three germ layers. (
  • as fibroblast-like cells that reside in the bone marrow of vertebrate animals, including humans. (
  • Several investigators have reported the isolation of primitive human adult multipotent cells from different tissues, including bone marrow. (
  • Although many of these primitive adult cell subpopulations have the potential to differentiate toward various cell lineages, it remains to be established if a particular population of adult human stem cells can maintain a broad and multilineage differentiation capacity, resembling the plasticity of embryonic stem cells, throughout the life of the individual. (
  • This virus persists in bone marrow mesenchymal stem cells (HBMSCs) of some immunocompetent individuals several years after primary infection. (
  • Due the critical role of HBMSCs in bone marrow niche and inhibitory effect of inflammatory cytokines on hematopoiesis, the aim of this study was to investigate the effect of B19V on IL-6 and TNF-α gene expression intransfected cells. (
  • After 24 h of transfection, quantitative mRNA expression of IL-6 and TNF-α was evaluated and human cord blood CD34+ HSC were cultured with the transfected cells. (
  • Several cytokines, especially granulocyte/macrophage colony-stimulating factor (GM-CSF) and tumor necrosis factor alpha (TNF-alpha), have been identified that foster the development of dendritic cells from blood and bone marrow precursors in suspension cultures. (
  • The addition of c-kit-ligand to GM-CSF- and TNF-alpha-supplemented suspensions of CD34+ bone marrow cells expanded CFU-DCs almost 100-fold by 14 d. (
  • Thus, the precise role of IL-6 and its intracellular signaling pathways for survival of human MM cells is still unclear. (
  • Here we show that treatment of human MM cells (IL-6-dependent MM cell line INA-6 and primary MM cells) with the IL-6 receptor antagonist Sant7 or with an anti-gp130 monoclonal antibody (mAb) induced apoptosis if the cells were cultured in the absence of bone marrow stromal cells (BMSCs). (
  • These observations indicate that the IL-6/gp130/STAT3 pathway is not essential for survival of human myeloma cells if they are grown in the presence of cells from the bone marrow microenvironment. (
  • Human bone marrow (BM)-derived stromal cells contain a population of skeletal stem cells (SSCs), with the capacity to differentiate along the osteogenic, adipogenic, and chondrogenic lineages, enabling their application to clinical therapies. (
  • However, there are no data, in human, about the combinatory use of silicate granules with autologous Bone Marrow Mononuclear cells (BMMCs) to refill bone cavity. (
  • We investigated lncRNAs in 979 human bone marrow-derived CD34 + cells by single cell RNA sequencing followed by de novo transcriptome reconstruction. (
  • Hence this study sought to assess the in vitro differentiation capacity of human bone marrow-derived stem cells (BMSC) towards retinal cell types. (
  • These cells were then cocultured on human RPE for 7 days. (
  • Our data demonstrated that human CD34 + CD38 - BMSC may differentiate towards an RPE-like cell type in vitro and could become a new type of donor cells for regenerative therapy in retinal degenerative diseases. (
  • We therefore performed a comprehensive assessment of human bone marrow cells using both single-cell RNA sequencing and multiparameter flow cytometry from 20 healthy adult human donors across a broad age range. (
  • Over recent years, a few types of mammalian cells have been successfully freeze-dried, such as human erythrocytes and human platelets (Crowe et al. (
  • With a lot of potential candidates to choose from Cairo University researchers decided to trial human bone marrow mesenchymal cells (BMSCs) in the human spinal cord. (
  • We have all heard about how neural "stem" cells can be prepared from embryonic tissue, but bone marrow is perhaps best known as a source of stem cells for patients with acute myeloid leukemia. (
  • To characterize the main population of bone marrow-derived cells (BMCs) in human normal subconjunctiva and make a comparison with BMCs in the corneal stroma and epithelium. (
  • These findings demonstrated that the main population of BMCs in the substantia propria of normal human conjunctiva is CD68 + CD14 + HLA-DR + cells. (
  • In addition to the existence of bone marrow-derived cells (BMCs) in normal mouse cornea, 2 3 4 we have previously characterized BMCs that reside in the normal human corneal stroma and epithelium. (
  • Flow cytometric analysis of the DNA content of bovine and human bone marrow cells. (
  • Mononucleated cells were isolated by gradient centrifugation, In the bone marrow samples from calves and cows, 35 +/- 2.6% and 31.8 +/- 1.5% of the isolated bone marrow cells respectively were in the S/G(2)/M-phase. (
  • In the human samples, only 12 +/- 0.8% of the cells were in the S/G(2)/M-phase. (
  • These results indicated that the proliferative, in activity of haematopoietic cells is significantly higher in cattle than in humans. (
  • Hoeben D, Burvenich C, Lenjou M, Nijs G, Massart-Leën A, Van Bockstaele D. Flow cytometric analysis of the DNA content of bovine and human bone marrow cells. (
  • Bone marrow mesenchymal stem cells inhibit the response of naive and memory antigen-specific T cells to their cognate peptide. (
  • Human mesenchymal stem cells modulate B-cell functions. (
  • Sotiropoulou PA, Perez SA, Gritzapis AD, Baxevanis CN, Papamichail M. Interactions between human mesenchymal stem cells and natural killer cells. (
  • Biologic properties of mesenchymal stem cells derived from bone marrow and adipose tissue. (
  • Our result clearly showed that GFAP+ cells activate TGF and induce hibernation of human HSPCs in mouse BM. (
  • To address this possibility in an experimental model of PD, bone marrow-derived neuroprogenitor-like cells were generated from bone marrow procured from healthy human adult volunteers and their potential to elicit recovery of damaged DA axons was studied in a partial lesion rat model of PD. (
  • Following collection of bone marrow, mesenchymal stem cells (MSC) were isolated and then genetically modified to create SB623 cells by transient transfection with the intracellular domain of the Notch1 gene (NICD), a modification that upregulates expression of certain neuroprogenitor markers. (
  • At 3 weeks, a small number of grafted SB623 cells survived in the lesioned striatum as visualized by expression of the human specific nuclear matrix protein (hNuMA). (
  • The role of hematopoietic stem cells in blood cell development is reasonably understood, whereas the identity and the function of bone marrow stromal cells are much less clear. (
  • Using stromal cells in bone marrow cultures of the Dexter type, a favorite medium for the study of hematopoiesis, we show that stromal cells actually represent a unique cell type. (
  • Multilineage gene expression in human bone marrow stromal cells as evidenced by single-cell microarray analysis. (
  • In vitro induction of alkaline phosphatase levels predicts in vivo bone forming capacity of human bone marrow stromal cells. (
  • A Pan-BCL2 inhibitor renders bone-marrow-resident human leukemia stem cells sensitive to tyrosine kinase inhibition. (
  • We compared the phenotype and function of virus-specific CD8(+) T cells from matched samples of human peripheral blood and bone marrow. (
  • Analysis of virus-specific memory CD8(+) T cells showed widely divergent partition of antigen-specific populations between blood and bone marrow. (
  • T cells specific for EBV lytic antigens were enriched three fold in marrow compared to blood whereas the response to EBV latent epitopes was equivalent between the two compartments. (
  • In direct contrast, although CMV-specific T cells were the largest virus-specific population within peripheral blood they were reduced by 60% within marrow. (
  • Bone marrow T cells were found to exhibit a unique CCR5(+)CXCR6(+)CXCR3(-) homing phenotype which has not been observed on T cells from other secondary lymphoid organs or peripheral organs. (
  • These observations identify a novel bone marrow homing phenotype for CD8(+) memory T cells, which necessitates a re-evaluation of the magnitude of antigen-specific populations within the lymphoid system. (
  • Chip-Based Comparison of the Osteogenesis of Human Bone Marrow- and Adipose Tissue-Derived Mesenchymal Stem Cells under Mechanical Stimulation. (
  • We compared human bone marrow-derived mesenchymal stem cells (hMSCs) and hASCs under dynamic hydraulic compression to evaluate and compare osteogenic abilities. (
  • Targeting ectodysplasin promotor by CRISPR/dCas9-effector effectively induces the reprogramming of human bone marrow-derived mesenchymal stem cells into sweat gland-like cells. (
  • The aim of the present study was to analyze the effect of 3 different expansion media on the expression of marker genes of mesenchymal differentiation (bone, cartilage, fat) as well as apoptosis and senescence during repeated passaging in human bone marrow stromal cells (hBMSCs) in order to identify potential expansion strategies for the use of these cells into tissue-engineered growth of bone. (
  • Cellular pharmacology of 3'-azido-3'-deoxythymidine with evidence of incorporation into DNA of human bone marrow cells. (
  • The present study evaluates the effect of toxic concentrations of AZT on possible sites of toxicity in human bone marrow cells. (
  • Nucleic acids of human bone marrow cells exposed for 24 hr to 10 microM [3H]AZT were purified and analyzed by cesium sulfate density gradient. (
  • In particular, a significant relationship (p less than 0.0001) between the level of AZT incorporated into DNA and the inhibition of clonal growth was observed at concentrations of AZT between 1 and 25 microM (IC50 and IC85 for human bone marrow cells). (
  • In summary, these studies demonstrate that AZT is incorporated into DNA of human bone marrow cells and suggest that incorporation of AZT into DNA may be one mechanism responsible for AZT-induced bone marrow toxicity. (
  • In contrast, imbalance of deoxyribonucleotide pools by AZT appears unlikely to be associated with inhibition of DNA synthesis and toxicity in human bone marrow cells. (
  • Treatment of myocardial infarction (MI) with bone marrow-derived mesenchymal stem cells and recently also adipose-derived stem cells has shown promising results. (
  • In contrast to clinical trials and their use of autologous bone marrow-derived cells from the ischemic patient, the animal MI models are often using young donors and young, often immune-compromised, recipient animals. (
  • Our objective was to compare bone marrow-derived mesenchymal stem cells with adipose-derived stem cells from an elderly ischemic patient in the treatment of MI using a fully grown non-immune-compromised rat model. (
  • Mesenchymal stem cells were isolated from adipose tissue and bone marrow and compared with respect to surface markers and proliferative capability. (
  • To compare the regenerative potential of the two stem cell populations, male Sprague Dawley rats were randomized to receive intramyocardial injections of adipose-derived stem cells, bone marrow-derived mesenchymal stem cells, or phosphate-buffered saline 1 week following induction of MI. (
  • Adipose-derived as well as bone marrow-derived mesenchymal stem cells prevented left ventricular end diastolic dilation. (
  • Adipose-derived stem cells from a human ischemic patient preserved cardiac function following MI, whereas this could not be demonstrated for bone marrow-derived mesenchymal stem cells, with only adipose-derived stem cells leading to an improvement in LVEF. (
  • Porcine small intestinal submucosa sheets as a scaffold for human bone marrow stem cells. (
  • In the present study, we investigated the possible genotoxic effects of SEM with respect to the following cytogenetic end-points: (1) in vitro micronucleus (MN) formation and sister-chromatid exchange (SCE) induction in human lymphocytes and (2) in vivo micronucleus induction on rat bone marrow polychromatic erythrocytes (PCEs). (
  • The paper is called "In vitro analog of human bone marrow from 3D scaffolds with Biomimetic inverted colloidal crystal geometry. (
  • Early evidence for the importance of IL-7 in murine B cell production came from in vitro studies showing that B cell precursors derived from adult bone marrow (BM) increase by ∼50-fold with the addition of IL-7 ( 10 ). (
  • found a rapid attachment and a homogenous distribution of both bovine and human chondrocytes on poly (ethylene oxide terephthalate)-co-poly (butylene terephthalate) (PEOT/PBT) based scaffolds after spinner flask culture in vitro . (
  • We hypothesized that identification of BM niche leads us to manipulate the function of human HSPCs in vivo and in vitro. (
  • Human marrow stromal cell treatment provides long-lasting benefit after traumatic brain injury in rats. (
  • This study was designed to investigate the effects of human bone marrow stromal cell (hMSC) administration in rats for 3 months after traumatic brain injury (TBI). (
  • Human bone marrow stromal cell: coexpression of markers specific for multiple mesenchymal cell lineages. (
  • The effect of a polyurethane-based reverse thermal gel on bone marrow stromal cell transplant survival and spinal cord repair. (
  • C ) Clonally isolated hBMSCs cultured for 120 PDs were labeled with PE- or FITC-conjugated Abs against human CD29, CD44, CD73, CD105, CD90, or CD117, or Ig isotype controls. (
  • For gene expression comparison, total RNA was extracted from hBMSCs using RNeasy Kit (Qiagen) and gene expression was examined with human Mesenchymal Stem Cell PCR Array (SABiosciences). (
  • article{131470, abstract = {The defence against infection in high-yielding dairy cows is correlated with the number and function of circulating neutrophils and depends on their production in bone marrow. (
  • Abstract 259: Role of Connexin 43 in Human Bone Marrow Derived Mesenchymal Stem Cell Cardiac Integration and Cardiac Stem cell Niche Formation. (
  • They have been identified and isolated from multiple tissues, including adipose tissue, umbilical cord, bone marrow, muscle, and fetal liver [ 4 ]. (
  • Unfortunately, none of the current proposed markers alone can isolate a homogeneous cell population with the ability to form bone, cartilage, and adipose tissue in humans. (
  • Therefore, the DNA content of isolated bone marrow cell suspensions from 7 calves, 7 cows and 14 humans was assayed by flow cytometry, Bovine sternal bone marrow samples were collected within 30 min of death, and human marrow samples were collected by sternal puncture and aspiration. (
  • The bones were used as 'cans' that preserved the bone marrow for a long period until it was time to take off the dry skin, shatter the bone and eat the marrow," Professor Barkai emphasizes. (
  • PCs migrate to bone marrow and inflamed tissues where they complete maturation into long-lived PCs that are resistant to immunosuppressive treatments. (
  • Until now, evidence has pointed to immediate consumption of marrow following the procurement and removal of soft tissues. (
  • However, because PBM circulate only briefly before entering the tissues and are difficult to infect with HCMV, it has been suggested that they may acquire HCMV during development in the bone marrow. (
  • This lymphocyte population mainly resides in the peripheral blood and bone marrow, although they have also been detected in primary and secondary lymphoid tissues ( 1 , 2 , 3 , 4 , 5 , 6 , 7 ). (
  • However, current methods to isolate and enrich SSCs from human tissues remain, at best, challenging in the absence of a specific SSC marker. (
  • 5 The leukocytes that normally reside in human conjunctival tissues were used as normal controls for investigation of pathologic conditions, such as cicatricial pemphigoid, 8 9 10 vernal keratoconjunctivitis, 11 12 pterygium, 13 14 trachoma, 15 and Behçet disease. (
  • e - g ) Human CD34 + cell rolling in a NOD/SCID collecting venule. (
  • 19 At least four of these mucin-like molecules are expressed at high level on primitive human HPCs, including CD34, 20 21 CD43, 22 23 CD45RA, 24 and PSGL-1 (CD162). (
  • However, most data concerning the role of IL-6 and IL-6-triggered signaling pathways were obtained from experiments performed with MM cell lines and without considering the bone marrow microenvironment. (
  • PEGylated Wnt3A liposomes associated with skeletal stem cell populations in human bone marrow and promoted osteogenesis. (
  • 1. Erythroblasts were enriched from human bone marrow samples and fractionated on Percoll gradients according to maturity. (
  • Peripheral blood monocytes (PBM) are one site of persistence of human cytomegalovirus (HCMV) in healthy carriers. (
  • The frequency of micronucleated reticulocytes (MN-RETs) in the bone marrow or peripheral blood is a sensitive indicator of cytogenetic damage. (
  • Samples of human bone marrow (BM) and mobilized peripheral blood (mPB) were collected from patients with blood cancer and granulocyte colony-stimulating factor (G-CSF) mobilization. (
  • This is the first successful artificial bone marrow," Kotov said. (
  • Anti-human CD117 antibody-mediated bone marrow niche clearance in nonhuman primates and humanized NSG mice. (
  • We investigated the effect of Notch signaling, a known regulator of cell fate in numerous developmental systems, on human hematopoietic precursors. (
  • These data suggest that Delta-1 can enhance myeloid and lymphoid marrow-repopulating ability and promote the generation of thymus-repopulating T cell precursors. (
  • Our data demonstrate that MSC from the two different sources respond similarly to inductive cues to differentiate terminally to a DA cell type, and the neuronal plasticity of human WJ MSC is comparable with that of BM MSC. (
  • To determine whether the substance behaves like real bone marrow, the scientists implanted it in mice with immune deficiencies. (
  • The researchers have developed an artificial tissue that mimics some of the complex biological properties of natural bone marrow niches. (
  • The new technique could also be used to produce tailor-made bone marrow niches that have specific molecular properties and that allow the selective incorporation or removal of individual proteins. (
  • METHODS Records of 87 patients who underwent allogeneic bone marrow transplantation were reviewed. (
  • These data provide evidence that IL-7 is essential for human B cell production from adult BM and that IL-7-induced expansion of the pro-B compartment is increasingly critical for human B cell production during the progression of ontogeny. (
  • Tel Aviv University researchers, in collaboration with scholars from Spain, have uncovered evidence of the storage and delayed consumption of animal bone marrow at Qesem Cave near Tel Aviv, the site of many major discoveries from the late Lower Paleolithic period some 400,000 years ago. (
  • In collaboration with scholars from Spain, the Tel Aviv University researchers found evidence the humans saved animal bones for up to nine weeks before feasting on them. (
  • Professor Ran Barkai , from Tel Aviv University, said: "Bone marrow constitutes a significant source of nutrition and as such was long featured in the prehistoric diet. (
  • For several years, researchers have been trying to reproduce natural bone marrow in the laboratory in order to better understand the mechanisms of blood formation and to develop new therapies - such as for the treatment of leukemia. (
  • The researchers contend that the deer metapodials were kept at the cave covered in skin to facilitate the preservation of marrow for consumption in time of need. (
  • The researchers evaluated the preservation of bone marrow using an experimental series on deer, controlling exposure time and environmental parameters, combined with chemical analyses. (
  • The new artificial marrow could allow researchers to test how a new drug at certain potencies would affect bone marrow function, Kotov said. (
  • The researchers demonstrated that the artificial marrow gives a human-like response to an infectious New Caledonia/99/H1N1 flu virus. (
  • Researchers have found evidence that humans living in Qesem Cave in Israel between 420,000 and 200,000 years ago stored long bones to eat later. (
  • Researchers have found evidence that humans living in Qesem Cave in Israel between 420,000 and 200,000 years ago stored long bones so they could tuck into the marrow later. (
  • For comparison, the researchers monitored changes in red deer marrow preservation and processing marks over a period of nine weeks. (
  • The Cairo researchers conclude that more studies will be needed before BMSCs can usefully be tested again in the human spinal cord. (
  • To study the molecular mechanisms involved in human leukemias and to improve treatment options, it is critically important to establish in vivo xenograft models that faithfully recapitulate the disease. (
  • Our data reveal valuable information on the kinetics of radiation-induced MN-RET of human bone marrow cultured in the 3D bioreactor, a synthetic bioculture system, and suggest that this model may serve as a promising tool for studying MN-RET formation in human bone marrow, thereby providing opportunities to study bone marrow genotoxicity testing, mitigating agent effects, and other conditions that are not ordinarily feasible to experimental manipulation in vivo. (
  • CONCLUSION: These results indicate that canonical Wnt signaling is involved in human BM B lymphopoiesis where it acts as a negative regulator of cell proliferation in a direct or stroma dependent manner. (
  • Arora P, Sindhu A, Dilbaghi N, Chaudhury A, Rajakumar G, Rahuman AA (2012) Nano-regenerative medicine towards clinical outcome of stem cell and tissue engineering in humans. (
  • While this was not a randomised clinical trial in the strictest sense, the study was well designed and the findings add significantly to previous observations made in isolated cases and smaller human trials. (
  • Whilst disappointing, it can be difficult to know when a particular therapy is ready for human clinical trials, and even negative findings can be powerful tools for unravelling responses. (
  • Viral infections caused by human adenovirus (HAdV) or CMV remain life-threatening complications in immunocompromised patients undergoing allogeneic hematopoietic stem cell transplantation. (
  • The effect of human leukocyte antigen disparity on cyclosporine neurotoxicity after allogeneic bone marrow transplantation. (
  • PURPOSE We examined the relationship between human leukocyte antigen (HLA) matching and the development of cyclosporine (CyA) neurotoxicity in patients undergoing allogeneic bone marrow transplantation, and determined the frequency and imaging characteristics of CyA neurotoxicity in these patients. (
  • Human umbilical vein ECs (lane 4) and immortalized cell lines with high telomere length, provided by the Roche Diagnostics Corp. (lane 5), were used as controls. (