beta-Lactamases: Enzymes found in many bacteria which catalyze the hydrolysis of the amide bond in the beta-lactam ring. Well known antibiotics destroyed by these enzymes are penicillins and cephalosporins.Cephaloridine: A cephalosporin antibiotic.Anti-Bacterial Agents: Substances that reduce the growth or reproduction of BACTERIA.Microbial Sensitivity Tests: Any tests that demonstrate the relative efficacy of different chemotherapeutic agents against specific microorganisms (i.e., bacteria, fungi, viruses).Carbapenems: A group of beta-lactam antibiotics in which the sulfur atom in the thiazolidine ring of the penicillin molecule is replaced by a carbon atom. THIENAMYCINS are a subgroup of carbapenems which have a sulfur atom as the first constituent of the side chain.Enterobacteriaceae: A family of gram-negative, facultatively anaerobic, rod-shaped bacteria that do not form endospores. Its organisms are distributed worldwide with some being saprophytes and others being plant and animal parasites. Many species are of considerable economic importance due to their pathogenic effects on agriculture and livestock.Enterobacteriaceae Infections: Infections with bacteria of the family ENTEROBACTERIACEAE.Acinetobacter baumannii: A species of gram-negative, aerobic bacteria, commonly found in the clinical laboratory, and frequently resistant to common antibiotics.beta-Lactam Resistance: Nonsusceptibility of bacteria to the action of the beta-lactam antibiotics. Mechanisms responsible for beta-lactam resistance may be degradation of antibiotics by BETA-LACTAMASES, failure of antibiotics to penetrate, or low-affinity binding of antibiotics to targets.Klebsiella pneumoniae: Gram-negative, non-motile, capsulated, gas-producing rods found widely in nature and associated with urinary and respiratory infections in humans.Klebsiella Infections: Infections with bacteria of the genus KLEBSIELLA.Acinetobacter Infections: Infections with bacteria of the genus ACINETOBACTER.beta-Lactams: Four-membered cyclic AMIDES, best known for the PENICILLINS based on a bicyclo-thiazolidine, as well as the CEPHALOSPORINS based on a bicyclo-thiazine, and including monocyclic MONOBACTAMS. The BETA-LACTAMASES hydrolyze the beta lactam ring, accounting for BETA-LACTAM RESISTANCE of infective bacteria.Bacterial Proteins: Proteins found in any species of bacterium.Thienamycins: Beta-lactam antibiotics that differ from PENICILLINS in having the thiazolidine sulfur atom replaced by carbon, the sulfur then becoming the first atom in the side chain. They are unstable chemically, but have a very broad antibacterial spectrum. Thienamycin and its more stable derivatives are proposed for use in combinations with enzyme inhibitors.Imipenem: Semisynthetic thienamycin that has a wide spectrum of antibacterial activity against gram-negative and gram-positive aerobic and anaerobic bacteria, including many multiresistant strains. It is stable to beta-lactamases. Clinical studies have demonstrated high efficacy in the treatment of infections of various body systems. Its effectiveness is enhanced when it is administered in combination with CILASTATIN, a renal dipeptidase inhibitor.Azabicyclo Compounds: Bicyclic bridged compounds that contain a nitrogen which has three bonds. The nomenclature indicates the number of atoms in each path around the rings, such as [2.2.2] for three equal length paths. Some members are TROPANES and BETA LACTAMS.Enterobacter cloacae: A species of gram-negative, facultatively anaerobic, rod-shaped bacteria that occurs in water, sewage, soil, meat, hospital environments, and on the skin and in the intestinal tract of man and animals as a commensal.Drug Resistance, Multiple, Bacterial: The ability of bacteria to resist or to become tolerant to several structurally and functionally distinct drugs simultaneously. This resistance may be acquired through gene mutation or foreign DNA in transmissible plasmids (R FACTORS).Multiplex Polymerase Chain Reaction: Methods for using more than one primer set in a polymerase chain reaction to amplify more than one segment of the target DNA sequence in a single reaction.Gram-Negative Bacteria: Bacteria which lose crystal violet stain but are stained pink when treated by Gram's method.Drug Resistance, Bacterial: The ability of bacteria to resist or to become tolerant to chemotherapeutic agents, antimicrobial agents, or antibiotics. This resistance may be acquired through gene mutation or foreign DNA in transmissible plasmids (R FACTORS).Hospitals, Teaching: Hospitals engaged in educational and research programs, as well as providing medical care to the patients.Multilocus Sequence Typing: Direct nucleotide sequencing of gene fragments from multiple housekeeping genes for the purpose of phylogenetic analysis, organism identification, and typing of species, strain, serovar, or other distinguishable phylogenetic level.Acinetobacter: A genus of gram-negative bacteria of the family MORAXELLACEAE, found in soil and water and of uncertain pathogenicity.Hospitals, General: Large hospitals with a resident medical staff which provides continuous care to maternity, surgical and medical patients.Hospitals, University: Hospitals maintained by a university for the teaching of medical students, postgraduate training programs, and clinical research.Cross Infection: Any infection which a patient contracts in a health-care institution.Electrophoresis, Gel, Pulsed-Field: Gel electrophoresis in which the direction of the electric field is changed periodically. This technique is similar to other electrophoretic methods normally used to separate double-stranded DNA molecules ranging in size up to tens of thousands of base-pairs. However, by alternating the electric field direction one is able to separate DNA molecules up to several million base-pairs in length.Hospitals: Institutions with an organized medical staff which provide medical care to patients.Gram-Negative Bacterial Infections: Infections caused by bacteria that show up as pink (negative) when treated by the gram-staining method.Interleukin-1beta: An interleukin-1 subtype that is synthesized as an inactive membrane-bound pro-protein. Proteolytic processing of the precursor form by CASPASE 1 results in release of the active form of interleukin-1beta from the membrane.Hospital Costs: The expenses incurred by a hospital in providing care. The hospital costs attributed to a particular patient care episode include the direct costs plus an appropriate proportion of the overhead for administration, personnel, building maintenance, equipment, etc. Hospital costs are one of the factors which determine HOSPITAL CHARGES (the price the hospital sets for its services).Minocycline: A TETRACYCLINE analog, having a 7-dimethylamino and lacking the 5 methyl and hydroxyl groups, which is effective against tetracycline-resistant STAPHYLOCOCCUS infections.Bacteriological Techniques: Techniques used in studying bacteria.Molecular Typing: Using MOLECULAR BIOLOGY techniques, such as DNA SEQUENCE ANALYSIS; PULSED-FIELD GEL ELECTROPHORESIS; and DNA FINGERPRINTING, to identify, classify, and compare organisms and their subtypes.Hospitals, Urban: Hospitals located in metropolitan areas.Nursing Staff, Hospital: Personnel who provide nursing service to patients in a hospital.Economics, Hospital: Economic aspects related to the management and operation of a hospital.Hospitals, Pediatric: Special hospitals which provide care for ill children.DNA, Bacterial: Deoxyribonucleic acid that makes up the genetic material of bacteria.DNA Fingerprinting: A technique for identifying individuals of a species that is based on the uniqueness of their DNA sequence. Uniqueness is determined by identifying which combination of allelic variations occur in the individual at a statistically relevant number of different loci. In forensic studies, RESTRICTION FRAGMENT LENGTH POLYMORPHISM of multiple, highly polymorphic VNTR LOCI or MICROSATELLITE REPEAT loci are analyzed. The number of loci used for the profile depends on the ALLELE FREQUENCY in the population.Polymerase Chain Reaction: In vitro method for producing large amounts of specific DNA or RNA fragments of defined length and sequence from small amounts of short oligonucleotide flanking sequences (primers). The essential steps include thermal denaturation of the double-stranded target molecules, annealing of the primers to their complementary sequences, and extension of the annealed primers by enzymatic synthesis with DNA polymerase. The reaction is efficient, specific, and extremely sensitive. Uses for the reaction include disease diagnosis, detection of difficult-to-isolate pathogens, mutation analysis, genetic testing, DNA sequencing, and analyzing evolutionary relationships.beta 2-Microglobulin: An 11-kDa protein associated with the outer membrane of many cells including lymphocytes. It is the small subunit of the MHC class I molecule. Association with beta 2-microglobulin is generally required for the transport of class I heavy chains from the endoplasmic reticulum to the cell surface. Beta 2-microglobulin is present in small amounts in serum, csf, and urine of normal people, and to a much greater degree in the urine and plasma of patients with tubular proteinemia, renal failure, or kidney transplants.Hospital Bed Capacity: The number of beds which a hospital has been designed and constructed to contain. It may also refer to the number of beds set up and staffed for use.Hospitals, Special: Hospitals which provide care for a single category of illness with facilities and staff directed toward a specific service.Hospitals, District: Government-controlled hospitals which represent the major health facility for a designated geographic area.Sequence Analysis, DNA: A multistage process that includes cloning, physical mapping, subcloning, determination of the DNA SEQUENCE, and information analysis.Pseudomonas aeruginosa: A species of gram-negative, aerobic, rod-shaped bacteria commonly isolated from clinical specimens (wound, burn, and urinary tract infections). It is also found widely distributed in soil and water. P. aeruginosa is a major agent of nosocomial infection.Hospitals, Private: A class of hospitals that includes profit or not-for-profit hospitals that are controlled by a legal entity other than a government agency. (Hospital Administration Terminology, AHA, 2d ed)Financial Management, Hospital: The obtaining and management of funds for hospital needs and responsibility for fiscal affairs.Sensitivity and Specificity: Binary classification measures to assess test results. Sensitivity or recall rate is the proportion of true positives. Specificity is the probability of correctly determining the absence of a condition. (From Last, Dictionary of Epidemiology, 2d ed)Plasmids: Extrachromosomal, usually CIRCULAR DNA molecules that are self-replicating and transferable from one organism to another. They are found in a variety of bacterial, archaeal, fungal, algal, and plant species. They are used in GENETIC ENGINEERING as CLONING VECTORS.Emergency Service, Hospital: Hospital department responsible for the administration and provision of immediate medical or surgical care to the emergency patient.Length of Stay: The period of confinement of a patient to a hospital or other health facility.Hospital Planning: Areawide planning for hospitals or planning of a particular hospital unit on the basis of projected consumer need. This does not include hospital design and construction or architectural plans.Hospital Charges: The prices a hospital sets for its services. HOSPITAL COSTS (the direct and indirect expenses incurred by the hospital in providing the services) are one factor in the determination of hospital charges. Other factors may include, for example, profits, competition, and the necessity of recouping the costs of uncompensated care.Receptors, Adrenergic, beta: One of two major pharmacologically defined classes of adrenergic receptors. The beta adrenergic receptors play an important role in regulating CARDIAC MUSCLE contraction, SMOOTH MUSCLE relaxation, and GLYCOGENOLYSIS.Integrin beta3: An integrin beta subunit of approximately 85-kDa in size which has been found in INTEGRIN ALPHAIIB-containing and INTEGRIN ALPHAV-containing heterodimers. Integrin beta3 occurs as three alternatively spliced isoforms, designated beta3A-C.Hospital Departments: Major administrative divisions of the hospital.Hospitalization: The confinement of a patient in a hospital.Retrospective Studies: Studies used to test etiologic hypotheses in which inferences about an exposure to putative causal factors are derived from data relating to characteristics of persons under study or to events or experiences in their past. The essential feature is that some of the persons under study have the disease or outcome of interest and their characteristics are compared with those of unaffected persons.Hospitals, Psychiatric: Special hospitals which provide care to the mentally ill patient.Genotype: The genetic constitution of the individual, comprising the ALLELES present at each GENETIC LOCUS.Hospital Records: Compilations of data on hospital activities and programs; excludes patient medical records.Hospital Units: Those areas of the hospital organization not considered departments which provide specialized patient care. They include various hospital special care wards.Equipment and Supplies, Hospital: Any materials used in providing care specifically in the hospital.Libraries, Hospital: Information centers primarily serving the needs of hospital medical staff and sometimes also providing patient education and other services.Transforming Growth Factor beta: A factor synthesized in a wide variety of tissues. It acts synergistically with TGF-alpha in inducing phenotypic transformation and can also act as a negative autocrine growth factor. TGF-beta has a potential role in embryonal development, cellular differentiation, hormone secretion, and immune function. TGF-beta is found mostly as homodimer forms of separate gene products TGF-beta1, TGF-beta2 or TGF-beta3. Heterodimers composed of TGF-beta1 and 2 (TGF-beta1.2) or of TGF-beta2 and 3 (TGF-beta2.3) have been isolated. The TGF-beta proteins are synthesized as precursor proteins.Surgery Department, Hospital: Hospital department which administers all departmental functions and the provision of surgical diagnostic and therapeutic services.Patient Admission: The process of accepting patients. The concept includes patients accepted for medical and nursing care in a hospital or other health care institution.Patient Discharge: The administrative process of discharging the patient, alive or dead, from hospitals or other health facilities.Outpatient Clinics, Hospital: Organized services in a hospital which provide medical care on an outpatient basis.Hospitals, County: Hospitals controlled by the county government.
Both narrow spectrum beta-lactamases (e.g. penicillinases) and extended spectrum beta-lactamases (ESBL) are common for ... KPC, NDM-1, VIM and OXA-48 carbapenemases have been increasingly reported worldwide as causes of hospital-acquired infections. ... Often multiple beta-lactamase genes are found on the same plasmid hydrolyzing a wide spectrum of beta-lactam antibiotics. ESBL ... The first clinically observed ESBL enzymes were mutated versions of the narrow spectrum beta-lactamases, like TEM and SHV. ...
The gene for NDM-1 is one member of a large gene family that encodes beta-lactamase enzymes called carbapenemases. Bacteria ... She had been on an extended trip to India and was admitted to a hospital there for an infected right hip. Detection of NDM-1 ... New Delhi metallo-beta-lactamase 1 (NDM-1) is an enzyme that makes bacteria resistant to a broad range of beta-lactam ... The NDM-1 enzyme is one of the class B metallo-beta-lactamase; other types of carbapenemase are class A or class D beta- ...
Carbapenems are famously stable to AmpC β-lactamases and extended-spectrum-β-lactamases. Carbapenemases are a diverse group of ... The prevalence of ESBL-producing bacteria have been gradually increasing in acute care hospitals. ESBLs are beta-lactamases ... Beta-lactamases are ancient bacterial enzymes. The class B beta-lactamases (the metallo-beta-lactamases) are divided into three ... The OXA beta-lactamase family was originally created as a phenotypic rather than a genotypic group for a few beta-lactamases ...
... the percentage of Escherichia coli isolates from Canadian hospitals that produce extended spectrum beta lactamases (ESBL) ... Livermore DM, Woodford N (October 2000). "Carbapenemases: a problem in waiting?". Current Opinion in Microbiology. 3 (5): 489- ... including beta lactamases. Pseudomonas produces an inducible broad spectrum beta lactamase, AmpC, that is produced in response ... Beta lactam resistance in these pathogens is most commonly due to the expression of beta lactamase enzymes. Between 2007 and ...
Metallo-beta-lactamase-producing Enterobacteriaceae isolates in a university hospital in Taiwan: prevalence of IMP-8 in ... Agents Chemother.37(5),939-946 (1993). 81.Queenan AM, Bush K. Carbapenemases: the versatile beta-lactamases. Clin. Microbiol. ... Control of a prolonged outbreak of extended-spectrum beta-lactamase-producing Enterobacteriaceae in a university hospital. Clin ... Metallo-beta-lactamases: a last frontier for beta-lactams? Lancet Infect. Dis.11(5),381-393 (2011). 86.Deshpande LM, Jones RN, ...
Class D carbapenemases, also referred to as the OXA β-lactamases, are serine β-lactamases. They are encoded on plasmids and ... Hospital staff should be trained to never dispose of clinical waste down the sinks in patient rooms. A hospital in Melbourne, ... New Delhi Metallo-beta-lactamase). KPC and NDM are enzymes that break down carbapenems and make them ineffective. Both of these ... class B metallo-β-lactamases (MBL), and class D β-lactamases (OXA). The four known groups of class A carbapenemases are: SME ( ...
Beta-lactamases (such as penicillinases) formed by gram-positive and gram-negative bacteria can stabilize doripenem to ... but it is susceptible to the action of carbapenemases. Doripenem is also more active against Pseudomonas aeruginosa than other ... pneumonia within the setting of a hospital, and complicated infections of the urinary tract including kidney infections with ... However, carbapenem-hydrolyzing beta-lactamases are an exception. On average, about 8.1% of plasma proteins attached to ...
Klebsiella possesses beta-lactamase giving it resistance to ampicillin, many strains have acquired an extended-spectrum beta- ... It is typically due to aspiration and alcoholism may be a risk factor, though it is also commonly implicated in hospital- ... Hirsch EB, Tam VH (June 2010). "Detection and treatment options for Klebsiella pneumoniae carbapenemases (KPCs): an emerging ... In 2009, strains of K. pneumoniae with gene called New Delhi metallo-beta-lactamase ( NDM-1) that even gives resistance against ...
"Detection of Enterobacteriaceae isolates carrying metallo-beta-lactamase - United States, 2010" (PDF). Morbidity and Mortality ... Livermore DM, Woodford N (2000). "Carbapenemases: a problem in waiting?". Current Opinion in Microbiology 3 (5): 489-495. ISSN ... "New 'superbug' found in UK hospitals". BBC News. Pridobljeno dne 11. 8. 2010 ... Birnbaum J, Kahan FM, Kropp H, MacDonald JS (1985). "Carbapenems, a new class of beta-lactam antibiotics. Discovery and ...
... which is commonly used to inhibit bacterial beta-lactamase, but this is an example of the antibacterial property of sulbactam ... "Detection of OXA-type carbapenemases and integrons among carbapenem-resistant Acinetobactor baumannii in a Teaching Hospital in ... Of the Acinetobacter, A. baumannii is the greatest cause of human disease, having been implicated in a number of hospital- ... "Air ionizers wipe out hospital infections". The New Scientist. Retrieved 2006-08-30. Palmen R, Vosman B, Buijsman P, Breek CK, ...
A single GES-5-producing isolate co-produced the extended-spectrum β-lactamase BEL-1; both corresponding genes were co-located ... recovered during 2013-2018 in a 123-bed hospital in Lisbon. We identified blaKPC-3 (n = 36), blaOXA-181 (n = 9), and blaGES-5 ( ... Carbapenemases: the versatile beta-lactamases. Clin Microbiol Rev. 2007;20:440-58. DOIPubMed ... Plasmid-determined AmpC-type beta-lactamases. Antimicrob Agents Chemother. 2002;46:1-11. DOIPubMed ...
The emerging NDM carbapenemases. Trends Microbiol 2011;19:588-95. * Ben-David D, Maor Y, Keller N, et al. Potential role of ... Results were negative for a rectal swab obtained March 30 from the next patient who occupied the same hospital room on March 28 ... New Delhi metallo-beta-lactamase 1-producing Enterobacteriaceae: emergence and response in Europe. Euro Surveill 2010;15:pii= ... Characterization of a new metallo-beta-lactamase gene, blaNDM-1, and a novel erythromycin esterase gene carried on a unique ...
Imipenem against Clinical Extended Spectrum Beta-Lactamase-Producing Enterobacteriaceae Collected in Military Teaching Hospital ... V. Miriagou, G. Cornaglia, M. Edelstein et al., "Acquired carbapenemases in Gram-negative bacterial pathogens: detection and ... G. A. Jacoby and L. S. Munoz-Price, "The new beta-lactamases," The New England Journal of Medicine, vol. 352, no. 4, pp. 380- ... D. C. Lye, L. Wijaya, J. Chan, P. T. Chew, and S. L. Yee, "Ertapenem for treatment of extended-spectrum beta-lactamase- ...
Distribution of extended-spectrum beta-lactamases, AmpC beta-lactamases, and carbapenemases among Enterobacteriaceae isolates ... Strains were collected from the Clinical Microbiology Laboratory at Brigham and Womens Hospital (BWH), a 793-bed hospital in ... Carbapenemases: the versatile beta-lactamases. Clin Microbiol Rev 20:440-458. doi:10.1128/CMR.00001-07. ... Genomic context of carbapenemases and other beta-lactamases.Among the isolates sequenced, 17 strains had a carbapenemase gene ...
In recent years, the number of class D beta-lactamases with carbapenem-hydrolysing properties has increased substantially. ... Department of Clinical Microbiology, 9301, Rigshospitalet, The National University Hospital, Copenhagen, Denmark. [email protected] ... A putative ancestor to one of the plasmid-encoded OXA-type carbapenemases has been found. OXA-type carbapenemases are not ... and they are only remotely related to other class D beta-lactamases. ...
... metallo-beta-lactamases and for the carO gene encoding an outer membrane protein. PFGE was used to define clonal relatedness. ... baumannii isolates collected between January 2005 and June 2007 were screened for genes encoding OXA carbapenemases, ... Department of Internal Medicine, Kaohsiung Medical University Hospital, Kaohsiung City, Taiwan.. Abstract. OBJECTIVES: We ... Diversity of carbapenem resistance mechanisms in Acinetobacter baumannii from a Taiwan hospital: spread of plasmid-borne OXA-72 ...
The most common resistance mechanisms were KPC (K. pneumoniae carbapenemases) beta-lactamases encoded by blaKPC2, blaKPC3, and ... There was very little evidence of extensive hospital outbreaks, but a great deal of variation in resistance mechanisms and the ... Our broad sample of species and genetic determinants that defined four hospital CRE communities over 16 mo revealed a ... We prospectively sampled CRE from hospitalized patients from three Boston-area hospitals, together with a collection of CRE ...
Both narrow spectrum beta-lactamases (e.g. penicillinases) and extended spectrum beta-lactamases (ESBL) are common for ... KPC, NDM-1, VIM and OXA-48 carbapenemases have been increasingly reported worldwide as causes of hospital-acquired infections. ... Often multiple beta-lactamase genes are found on the same plasmid hydrolyzing a wide spectrum of beta-lactam antibiotics. ESBL ... The first clinically observed ESBL enzymes were mutated versions of the narrow spectrum beta-lactamases, like TEM and SHV. ...
Some of these carbapenemases include veron integron metallo-beta-lactamases, imipenemase, Klebsiella pneumoniae carbapenemases ... and NDM-type metallo-beta-lactamases, the class A KPC-type carbapenemases, and the class D OXA-48 enzyme. Of 227 isolates, 80 ( ... and New Delhi metallo-beta-lactamase_1 which are encoded by what is termed carbapenem resistance determining genes (CRDG): , , ... A. M. Queenan and K. Bush, "Carbapenemases: the versatile β-lactamases," Clinical Microbiology Reviews, vol. 20, no. 3, pp. 440 ...
This is Antabios second program to receive QIDP status, following QIDP designation of its Metallo Beta-Lactamase Inhibitor ... QIDP is granted to Antabios MEM-ANT3310 for major hospital indications including nosocomial pneumonia ... which displays excellent inhibitory activity against both KPC and OXA carbapenemases. It will be administered intravenously in ... ANT3310 is a novel, potent and specific inhibitor of bacterial Serine Beta-Lactamases (SBLs) currently in preclinical ...
... lactamase (ESBL)-producing Enterobacteriaceae is increasing worldwide. The influx of these bacteria into hospitals has major ... RESULTS Of 80 Enterobacteriaceae isolates from blood samples obtained at admission to the hospital, 13.7% produced ESBL. Thirty ... BACKGROUND The prevalence of infections caused by extended-spectrum beta - ... Distribution of extended-spectrum β-lactamases, AmpC β-lactamases, and carbapenemases among Enterobacteriaceae isolates causing ...
Indeed, it appears that the emergence of ESBL and carbapenemase producers in animals is not related to the use of beta lactam ... Isolated multi-drug resistant bacteria were often co-resistant to non-beta lactam antibiotics, frequently used in veterinary ... Isolated multi-drug resistant bacteria were often co-resistant to non-beta lactam antibiotics, frequently used in veterinary ... antibiotics but is, rather, due to the co-selective pressure applied by the over usage of non-beta lactams. The level of ...
Simultaneously, diverse strains harbouring New Delhi metallo-beta-lactamase (NDM-1) are repeatedly being imported into Europe, ... Since the nature and scale of carbapenem-non-susceptible Entrobacteriaceae as a threat to hospital patients in Europe remains ... A genetically distinct strain of Klebsiella pneumoniae sequence type (ST) 258 harbouring the K. pneumoniae carbapenemases (KPC ... Some of the resistance genes are associated with successful clonal lineages which have a selective advantage in those hospitals ...
New Delhi metallo-beta-lactamase 1-producing Enterobacteriaceae: emergence and response in Europe M J Struelens, D L Monnet, A ... Patients' demographic, infection-related and AMT-related data were collected from hospital wards. A total of 19 hospitals ... Acquired carbapenemases confer extensive antibiotic resistance to Enterobacteriaceae and represent a public health threat. A ... Simultaneously, diverse strains harbouring New Delhi metallo-beta-lactamase (NDM-1) are repeatedly being imported into Europe, ...
... the objectives of our study was also to screen for the occurrence of these beta-lactamases and carbapenemases in our hospital ... Beta-lactamase production is the most common mechanism of beta-lactam drug resistance in Gram-negative bacteria. Newer beta- ... 2 were AmpC beta-lactamase producers and no isolates were metallo-beta-lactamase producers. Despite the low prevalence of A. ... AmpC disk test for detection of plasmid-mediated AmpC beta-lactamases in Enterobacteriaceae lacking chromosomal AmpC beta- ...
... and metalo-beta-lactamases (MβLs)]. The aim of this work was to evaluate the production of OXA- and MβL-like carbapenemases, in ... Acinetobacter baumannii (Ab) is a leading cause of hospital infection, mainly in intensive care units. The main characteristic ... e metalo-beta-lactamases (MβL)]. O objetivo do presente trabalho foi avaliar a produção de enzimas carbapenemases do tipo OXA e ... Prevalência de genes codificadores de carbapenemases em isolados multirresistentes de Acinetobacter baumannii recuperados de ...
For the first time in Nepal, high prevalence of blaCTX-M-type ESBL and co-existence of ESBLs and carbapenemases has been ... A cross sectional study was conducted between June 2012 to January 2013 in Kathmandu Medical College and Teaching Hospital, ... Nine isolates possessed more than one type of beta-lactamases. Interestingly, all carbapenemase producers were isolated form ... blaVIM and blaNDM-1 beta-lactamases was documented in one ESBL-EC (EC104). All most all isolates had different RAPD patterns. ...
... medical centers during the MYSTIC Program 2007 were evaluated to determine the presence of selected beta-lactamase genes. CTX-M ... A total of 220 gram-negative isolates showing distinct beta-lactam resistance profiles recovered in U.S. ... and serine carbapenemases, that compromised the treatment of gram-negative infections in numerous U.S. hospitals participating ... of the extended spectrum beta-lactamase-positive isolates and were observed in 80.0% of the participating hospitals. CTX-M-14 ...
MRSA produces a beta-lactamase that fends off drugs like penicillin and cefazolin. In healthy individuals, MRSA can cause a ... increasingly prevalent in hospitals and may soon overtake hospital-acquired strains as the main cause of MRSA in hospital ... Carbapenemases. In the United States, Klebsiella pneumoniae is the most common bacteria that produces carbapenamase. Pneumonia ... Penicillinase is a beta-lactamase enzyme that breaks down (and thus resists) penicillin and other antibiotics. In fact, the ...
The spread of extended-spectrum beta-lactamase (ESBL)-producing organisms has gradually increased in hospitals and long-term ... AmpC beta-lactamases (DHA, MOX, and CMY), and carbapenemases (KPC, NDM, IMP, VIM, and OXA-48) using primers previously ... Beta-Lactam/beta-lactam inhibitor combinations for the treatment of bacteremia due to extended-spectrum beta-lactamase- ... Increasing incidence of extended-Spectrum beta-Lactamase-producing Escherichia coli in community hospitals throughout the ...
... pneumoniae producing both SHV-12-type extended-spectrum beta-lactamase and DHA-1-type AmpC beta-lactamase at a Korean hospital ... Livermore DM, Woodford N. Carbapenemases: a problem in waiting? Curr Opin Microbiol. 2000;3:489-495. [PubMed] ... VIM- and IMP-type metallo-beta-lactamase-producing Pseudomonas spp. and Acinetobacter spp. in Korean hospitals. Emerg Infect ... Outbreaks of imipenem resistant Acinetobacter baumannii producing OXA-23 beta-lactamase in a tertiary care hospital in Korea. ...
Phenotypic detection of carbapenem resistance and metallo-beta-lactamase among Enterobacteriaceae from clinical samples in ... Study of the prevalence of metallo-β-lactamase producing Enterobacteriacae from a tertiary care hospital in Mumbai. J Applied ... The emerging NDM carbapenemases. Trends Microbiol. 2011;19(12):588-95.. Balan K, Sireesha P, Setty CR. Study to detect ... of carbapenem resistance and metallo-beta-lactamase among Enterobacteriaceae from clinical samples in tertiary care hospital. ...
... but not plasmid-mediated Ambler class B beta-lactamases, were found in Estonian hospitals. This trial was registered ... Clinically relevant beta-lactamases (OXA-101, OXA-2 and GES-5) were found in 12% of strains, 27% of which were located in ... The presence of resistance genes and beta-lactamases and their origin was determined. Combined-disk method and PCR was used to ... No Ambler class B beta-lactamases were detected. Aminoglycoside modifying enzymes were found in 15% of the strains. OprD was ...
Actively screened CP non-susceptible strains as well as 367 archived ESBL/AmpC-β-lactamase-producing Enterobacteriaceae were ... Actively screened CP non-susceptible strains as well as 367 archived ESBL/AmpC-β-lactamase-producing Enterobacteriaceae were ... Carbapenemases are a diverse group of β-lactamases that hydrolyze not only CP but also other beta-lactam antibiotics. Among the ... Survey of clinical extended-spectrum beta-lactamase-producing Enterobacter cloacae isolates in a Chinese tertiary hospital, ...
KlebsiellaESBLCarbapenemsGenesAmpCHydrolyzeInhibitorIncluding extended-spectrumSusceptibilityMBLsAcinetobacterEscherichiaPathogensCephalosporinsEnzymeGramBroad-spectrumMolecularInfectionESBLs and CarbapenemasesEpidemiologyMechanismsBaumanniiClinicallyDetectionDisseminationPatientsAntimicrobial resistanceWeak carbapenemase activityTertiaryColiProducersIntegron
- We aimed to provide updated epidemiologic data on carbapenem-resistant Klebsiella pneumoniae in Portugal by characterizing all isolates (N = 46) recovered during 2013-2018 in a 123-bed hospital in Lisbon. (cdc.gov)
- Klebsiella pneumoniae is a major cause of hospital-acquired infections, mainly responsible for urinary, respiratory, and bloodstream infections, as well as infections in intensive care unit (ICU) patients. (cdc.gov)
- A urine specimen collected on March 4, 2012, from a patient who recently had been hospitalized in Viet Nam, but who was receiving care at a hospital in Rhode Island, was found to have a Klebsiella pneumoniae isolate containing NDM. (cdc.gov)
- Bacteremia due to Klebsiella pneumoniae isolates producing the TEM-52 extended-spectrum beta-lactamase: treatment outcome of patients receiving imipenem or ciprofloxacin," Clinical Infectious Diseases , vol. 38, no. 2, pp. 243-251, 2004. (hindawi.com)
- Antibi-otic therapy for Klebsiella pneumoniae bacteraemia: implications of production of extended-spectrum beta-lactamases," Clinical Infectious Diseases , vol. 39, pp. 31-37, 2003. (hindawi.com)
- Analysis of 41 isolates of Klebsiella pneumoniae and Enterobacter cloacae , collected over a 3-year period, identified K. pneumoniae carbapenemase (KPC) genes encoding KPC-2, −3, and −4 and OXA-48 carbapenemases. (asm.org)
- Members of Enterobacteriaceae family, for example, Escherichia coli or Klebsiella pneumoniae pose the biggest threat regarding plasmid-mediated resistance in hospital- and community-acquired infections. (wikipedia.org)
- Among the known carbapenemases are Klebsiella pneumoniae carbapenemase, or KPC, and Verona integrin-encoded metallo-Beta-lactamase, or VIM. (cdc.gov)
- A genetically distinct strain of Klebsiella pneumoniae sequence type (ST) 258 harbouring the K. pneumoniae carbapenemases (KPC) has been causing epidemics of national and international proportions. (eurosurveillance.org)
- The KPC serine carbapenemases were observed in the majority of the carbapenem-resistant Enterobacteriaceae (usually Klebsiella pneumoniae), confirming an epidemic problem in the New York City area. (nih.gov)
- Among the carbapenemase-producing isolates, 221 (15.5%) expressed carbapenemases other than Klebsiella pneumoniae carbapenemase. (cdc.gov)
- Carbapenem-resistant Escherichia coli and Klebsiella pneumoniae isolates from Turkey with OXA-48-like carbapenemases and outer membrane protein loss. (springermedizin.de)
- and more recently, the resistance to carbapenem antibiotics via the two most common mechanisms - Klebsiella Pneumoniae Carbapenemase (KPC), and New Delhi Metallobetalactamase-1 (NDM-1), which cause resistance to the broadest of all beta-lactam antibiotics. (infectiousdiseaseadvisor.com)
- RPX7009 is the first of a novel class of beta-lactamase inhibitors designed by Rempex Pharmaceuticals, a wholly owned subsidiary of The Medicines Company, to inhibit the KPC (Klebsiella pneumoniae carbapenemases) enzyme, the primary resistance mechanism to carbapenems. (themedicinescompany.com)
- Common examples are some of the most important Gram-negative human pathogens such as Klebsiella pneumoniae , Acinetobacter baumannii , Pseudomonas aeruginosa , and Escherichia coli , which harbor MGEs carrying genes encoding enzymes such as extended-spectrum beta-lactamases (ESBLs) that can hydrolyze penicillins, cephalosporins, and monobactams, along with aminoglycoside-modifying enzymes and the Qnr protection proteins that confer resistance to the fluoroquinolones ( 11 , 12 ). (asm.org)
- In hospital environments, Klebsiella species survive and multiply in wet environmental sites and colonize the human bowel, bladder, upper respiratory tract and skin [ 3 ]. (biomedcentral.com)
- During the 1990s, extended-spectrum beta-lactamases (ESBLs) producing Klebsiella species that are able to hydrolyze broad and extended-spectrum cephalosporins, monobactams and penicillins were reported [ 4 ]. (biomedcentral.com)
- K. pneumoniae was first reported to harbor Klebsiella pneumoniae carbapenemase (KPC), one of the epidemiologically important carbapenemases first detected in North Carolina, USA in 1996 [ 5 ]. (biomedcentral.com)
- A total of seventeen Klebsiella pneumoniae strains isolated within an eight-month period in 2013 since the first report of carbapenem resistant Enterobacteriaceae in this tertiary teaching hospital were revived from stock cultures. (biomedcentral.com)
- Ceftazidime/avibactam is a combination broad-spectrum cephalosporin (ceftazidime) and a novel beta-lactamase inhibitor (avibactam, formerly NXL104) to treat Gram-negative infections, including extended-spectrum beta-lactamases and Klebsiella pneumoniae carbapenemases that are resistant to existing therapies. (empr.com)
- Nine cases of Klebsiella pneumoniae NDM are described, as infectious or colonizing agents, in critically ill patients, mostly with neurosurgical pathology, of Hospital Nacional Dos de Mayo in Lima - Peru. (edu.pe)
- Phenotypic and genotypic characterization of antibiotic resistance in Klebsiella pneumoniae isolated from patients admitted to a third-level hospital in Tehran, Iran. (alliedacademies.org)
- Nowadays, Carbapenem resistant Klebsiella pneumoniae is one of the important factors in hospital infections. (alliedacademies.org)
- Klebsiella pneumoniae , Carbapenem, Metallo-beta-lactamase. (alliedacademies.org)
- Klebsiella is one of the commonly encountered gram-negative pathogens in hospital-acquired infection. (ijrhs.org)
- examples include Escherichia coli sequence type (ST)131 with CTX-M-15 ESBL and Klebsiella pneumoniae ST258 with KPC carbapenemases. (kjim.org)
- In contrast to Tn 4401 -based spread of Klebsiella pneumoniae carbapenemases (KPCs), diverse transposable elements mobilize NDM enzymes, commonly with other resistance genes, enabling naive strains to acquire multi- and extensively drug-resistant profiles with single transposition or plasmid conjugation events. (asm.org)
- Within the United States, Klebsiella pneumoniae carbapenemases (KPCs), serine beta-lactamases of the Ambler class A group, are the most commonly identified carbapenemase. (asm.org)
- The main objective is to better understand the distribution of bacteria and its resistance genes, Escherichia coli and Klebsiella pneumoniae bacteria and extended spectrum beta-lactamase (EsβL) and carbapenemases encoding genes in distinct ecological sources. (grandchallenges.org)
- Vaborbactam is a potent first-in-class inhibitor of class A (including the KPC, Klebsiella pneumoniae carbapenemase) and class C beta-lactamases, and it protects meropenem from degradation by serine carbapenemases, restoring its activity against carbapenem-resistant strains. (healthytimes.com.sg)
- The European Centre for Disease Prevention and Control has reported an outbreak of Klebsiella pneumoniae from the sequence type (ST) 307 producing two different carbapenemases (NDM-1 and OXA-48) first detected in Germany. (curetis.com)
- Since detection in Germany, this combination of the two resistance genes in Klebsiella pneumoniae has been found in one patient in Greece where the organism harbored NDM-1 and OXA-48 carbapenemases and 16 additional antimicrobial resistance genes . (curetis.com)
- During six weeks, once per week a screening was performed in hospitals were a patient carrying carbapenemase-producing and colistin resistant Klebsiella pneumoniae stayed overnight . (curetis.com)
- mergence of Klebsiella pneumoniae ST11 co-producing NDM-1 and OXA-48 carbapenemases in Greece. (curetis.com)
- This study included 33 NDMI-producing klebsiella pneumoniae that collected from different clinical specimens of patients admitted to different wards at Assiut University Hospitals in Egypt and confirmed to produce blaNDM1 gene by polymerase chain reaction. (sciepub.com)
- Emerging severe and fatal infections due to Klebsiella pneumoniae in two university hospitals in France. (sciepub.com)
- Characterization of a new metallo-β-lactamase gene, bla NDM-1, and a novel erythromycin esterase gene carried on a unique genetic structure in Klebsiella pneumoniae sequence type 14 from India. (sciepub.com)
- El-Sweify M, Gomaa N, El-Maraghy N, Mohamed H. Phenotypic Detection of Carbapenem Resistance among Klebsiella pneumoniae in Suez Canal University Hospitals, Ismailiya, Egypt. (sciepub.com)
- If testing for Klebsiella pneumoniae carbapenemase (KPC) or New Delhi metallo-beta-lactamase (NDM) is desired, order KNSRP / KPC (blaKPC) and NDM (blaNDM) Surveillance, PCR. (testcatalog.org)
- In the United States, Klebsiella pneumoniae carbapenemase (KPC) is the most common carbapenemase, followed by New Delhi metallo-beta-lactamase (NDM). (testcatalog.org)
- In addition, a few studies reported the carbapenemase GES-5 (a point mutant derivative of the extended-spectrum β-lactamase [ESBL] GES-1) in K. pneumoniae ( 5 ). (cdc.gov)
- Both narrow spectrum beta-lactamases (e.g. penicillinases) and extended spectrum beta-lactamases (ESBL) are common for resistance plasmids in Enterobacteriaceae. (wikipedia.org)
- ESBL enzymes can hydrolyze all beta-lactam antibiotics, including cephalosporins, except for the carpabepenems. (wikipedia.org)
- The first clinically observed ESBL enzymes were mutated versions of the narrow spectrum beta-lactamases, like TEM and SHV. (wikipedia.org)
- In addition, since the plasmids that carry ESBL genes also commonly encode resistance determinants for many other antibiotics, ESBL strains are often resistant to many non-beta-lactam antibiotics as well, leaving very few options for the treatment. (wikipedia.org)
- Carbapenemases represent type of ESBL which are able to hydrolyze carbapenem antibiotics that are considered as the last-resort treatment for ESBL-producing bacteria. (wikipedia.org)
- BACKGROUND The prevalence of infections caused by extended-spectrum beta -lactamase (ESBL)-producing Enterobacteriaceae is increasing worldwide. (semanticscholar.org)
- In recent years, extended ESBL and carbapenemase producing Gram negative bacteria have become widespread in hospitals, community settings and the environment. (frontiersin.org)
- Indeed, it appears that the emergence of ESBL and carbapenemase producers in animals is not related to the use of beta-lactam antibiotics but is, rather, due to the co-selective pressure applied by the over usage of non-beta-lactams. (frontiersin.org)
- The trend of extended-spectrum beta-lactamases producing Escherichia coli (ESBL-EC) is increasing in Nepal. (biomedcentral.com)
- Limited studies have been reported investigating ESBL types and carbapenemases in E. coli . (biomedcentral.com)
- Interestingly, all carbapenemase producers were isolated form ICU and co-existence of bla CTX-M , bla SHV , bla TEM , bla IMP , bla VIM and bla NDM-1 beta-lactamases was documented in one ESBL-EC (EC104). (biomedcentral.com)
- For the first time in Nepal, high prevalence of bla CTX-M -type ESBL and co-existence of ESBLs and carbapenemases has been described. (biomedcentral.com)
- there is paucity in studies of ESBL and carbapenemases types from this institution and Nepal. (biomedcentral.com)
- This study aims to detect common ESBLs ( bla TEM , bla SHV , and bla CTX-M ) and carbapenemases ( bla IMP , bla VIM , and bla NDM ) in ESBL-EC isolated from Kathmandu Medical College Teaching Hospital, Nepal. (biomedcentral.com)
- Here, we describe high prevalence of bla CTX-M type ESBL and carbapenemases producing E. coli and co-existence of ESBLs and carbapenemases in these isolates. (biomedcentral.com)
- We will focus on the spread of resistance genes that are amongst the most problematic now because they confer resistance to the latest generation of antibiotics available (genes for extended spectrum beta-lactamases (ESBL) and carbapenemases). (ku.dk)
- Actively screened CP non-susceptible strains as well as 367 archived ESBL/AmpC-β-lactamase-producing Enterobacteriaceae were then tested for the presence of CP genes by PCRs. (frontiersin.org)
- Clinical and microbiological characteristics of 348 K. pneumoniae bacteremia cases (2007-2009) were retrospectively characterized by multilocus sequence typing and extended-spectrum beta-lactamases (ESBL) production. (readbyqxmd.com)
- The prevalence of ESBL-producing bacteria have been gradually increasing in acute care hospitals. (wikipedia.org)
- These antibiotics are stable to undergo inactivation by beta-lactamases including the extended spectrum beta-lactamases (ESBL) and AMPc produced by most of the Gram negative pathogens. (maryelizabethbodycare.com)
- However, screening for carbapenemase production using chromogenic agar (Brilliance™ CRE agar and ChromID® CARBA agar), Modified Hodge test and amoxycillin-clavulanate double disc synergy test did not correlate with these resistance patterns and exhibited false positive results possibly due to the presence of extended spectrum beta-lactamase (ESBL) production by these organisms. (ukzn.ac.za)
- Extended Spectrum Beta Lactamase (ESBL) producing Escherichia coli is a global cause of life threatening infections. (scirp.org)
- Our findings show that although multidrug resistant ESBL producing E. coli are prevalent in both the hospital and the community in this environment, carbapenem resistance is still low. (scirp.org)
- Urinary tract infections (UTIs) caused by extended-spectrum β-lactamase (ESBL)-producing Enterobacteriaceae are resistant to many conventional therapies, including third-generation cephalosporins. (sma.org)
- A retrospective cohort study of adults with ESBL UTIs was conducted at a community hospital. (sma.org)
- In contrast to MRSA, ESBL producers - notably strains of Escherichia coli carrying plasmids of the CTX-M family - are mostly imported from the community into the hospital. (biomedcentral.com)
- The Carbapenems are β-lactam antibiotics that are used in the treatment of infections caused by Extended Spectrum beta-Lactamases (ESBL) producing gram negative bacteria (GNB). (ijcmas.com)
- The prevalence of ESBL and AmpC β-lactamase producers had also increased, indicating that the chance of encountering multidrugresistant pathogens would increase even in the scenario of community-sourced infection. (alliedacademies.org)
- Multidrug resistance associated with the production of enzymes such as Extended-Spectrum Beta-Lactamases (ESBL) and carbapenemases are being encountered with increasing frequency nowadays, leading to very limited therapeutic options. (ijrhs.org)
- These otics attributed to the production of extended-spectrum putative ESBL- and/or pAmpC-producers constituted the beta-lactamases (ESBLs) and/or plasmid-mediated study sample and were subjected to antimicrobial suscep- AmpC (pAmpC) enzymes [1, (deepdyve.com)
- Phenotypic and genotypic characterization of beta- Data on the epidemiology of ESBL- and pAmpC-- lactamases producing Enterobacteriaceae in Africa are still limited. (deepdyve.com)
- Carbapenems were the last line of treatment for most Extended Spectrum Beta Lactamase (ESBL) producing resistant organisms, however after the emergence of New Delhi Metallo-beta lactamase 1 (NDM-1) a recently evolved MBL has shown resistance to most classes of antibiotics including carbapenem except tigecycline, and colistin [ 10 ]. (omicsonline.org)
- However, data on resistance to third-generation cephalosporins, mostly via genes encoding extended-spectrum β-lactamase (ESBL) ( 2 , 3 ) or cephalosporinase determinants ( 4 ) are limited. (asm.org)
- Presently, the most serious threat is the increasing spread of Enterobacteriaceae carrying genes coding for extended spectrum beta-lactamases (ESBL) and carbapenemases on highly mobile plasmids. (chalmers.se)
- We use the assay to demonstrate a potential transmission route of an ESBL-carrying plasmid between bacterial strains/species and between patients, during a polyclonal outbreak at a neonatal ward at Sahlgrenska University Hospital (Gothenburg, Sweden). (chalmers.se)
- Both, NDM-1 and OXA-48, are often associated with the presence of other beta-lactamases such as extended-spectrum beta-lactamases (ESBL) or AmpC beta-lactamases. (curetis.com)
- Resistance to carbapenems among the MDR-GNB is mostly due to the production of carbapenemases, which are β -lactamases with capacity to hydrolyze not only the carbapenems themselves but also all the other beta lactam agents [ 5 ]. (hindawi.com)
- Carbapenemases can make germs resistant to some of our most powerful drugs, carbapenems. (cdc.gov)
- Carbapenems are a class of beta-lactam antibiotics that are capable of killing most bacteria by inhibiting the synthesis of one of their cell wall layers. (wikipedia.org)
- Metallo-beta-lactamase (MBL) activity has emerged as one of the most feared resistance mechanisms because of its ability to hydrolyze virtually all beta-lactams, including carbapenems. (beds.ac.uk)
- Chromosomal-mediated AmpC β-lactamases represent a new threat, since they confer resistance to 7-alpha-methoxy-cephalosporins (cephamycins) such as cefoxitin or cefotetan are not affected by commercially available β-lactamase inhibitors, and can, in strains with loss of outer membrane porins, provide resistance to carbapenems. (wikipedia.org)
- Carbapenems are a class of beta- lactam antibiotics primarily intended to use for the treatment of infections caused by GNB. (maryelizabethbodycare.com)
- Production of beta lactamases(carbapenemases) that hydrolyses carbapenems. (maryelizabethbodycare.com)
- Carbapenems are the drugs of choice for this pathogen and carbapenem-resistant A. baumannii has been frequently hospital encountered (13). (edu.iq)
- This was largely due to a rise in the use of beta-lactamase inhibitor combinations, such as Co-amoxiclav and Piptazobactam, as well as the carbapenems. (microbiologynutsandbolts.co.uk)
- The high frequency of MDR OXA-23-producing A. baumannii in various hospitals in Antananarivo is curious since carbapenems are not available in Madagascar, but it emphasises the need for infection control procedures and strict adherence to them to prevent the spread of these resistant organisms in Antananarivo and also the need to control the use of carbapenems in the future. (biomedcentral.com)
- As such, previously abandoned therapies, particularly carbapenems and beta-lactamase inhibitor combinations, may retain utility against infections caused by NDM producers. (nih.gov)
- Oxacillinase-48 (OXA-48) is a serine-beta-lactamase with hydrolytic activity against carbapenems and penicillins, but low or negligible activity against extended-spectrum cephalosporins. (curetis.com)
- In beta-lactamases that cannot break down carbapenems, this rearrangement cannot happen, and so the enzyme cannot break down the antibiotic. (infectioncontroltoday.com)
- Carbapenemases are β-lactamases that inactivate carbapenems. (cjur.ca)
- One mechanism of resistance to carbapenems is due to the carbapenem-hydrolyzing β-lactamases of molecular classes B and D ( 12 ). (asm.org)
- Rapid identification of the resistance genes, their mobilization capacity, and strains carrying them is essential to direct hospital resources to prevent spread and improve patient outcomes. (asm.org)
- OXA-type carbapenemases are not integrated into integrons as gene cassettes like many class D oxacillinases, but most of the OXA-type carbapenemases are instead encoded by chromosomal genes. (nih.gov)
- This article reviews the phylogeny and the genetic environments of the encoding genes and kinetic properties of the OXA-type carbapenemases. (nih.gov)
- The antibiotic resistance genes found on the plasmids confer resistance to most of the antibiotic classes used nowadays, for example, beta-lactams, fluoroquinolones and aminoglycosides. (wikipedia.org)
- Often multiple beta-lactamase genes are found on the same plasmid hydrolyzing a wide spectrum of beta-lactam antibiotics. (wikipedia.org)
- therefore, in this study, we determined the prevalence of carbapenemase genes among multidrug resistant gram negative bacteria (MDR-GNB) isolated from clinical specimens in a tertiary hospital in Mwanza, Tanzania. (hindawi.com)
- For each isolate, five different PCR assays were performed, allowing for the detection of the major carbapenemase genes, including those encoding the VIM-, IMP-, and NDM-type metallo-beta-lactamases, the class A KPC-type carbapenemases, and the class D OXA-48 enzyme. (hindawi.com)
- Prevalência de genes codificadores de carbapenemases em isolados multirresistentes. (usp.br)
- The presence of resistance genes and beta-lactamases and their origin was determined. (biomedcentral.com)
- Although main trigger associated with CR in PA is production of plasmid-mediated beta-lactamases/carbapenemases, mutational resistance mechanisms in chromosomal genes - e.g. altered expression of outer membrane porins or efflux systems and increased chromosomal cephalosporinase (AmpC) activity, may all have affected the development of resistance [ 7 ]. (biomedcentral.com)
- In this project, we will perform a cross-European examination of the fate of key resistant bacteria and resistance genes in UWSs resulting from discharged hospital and community wastes, including mechanisms of resistance gene transfer in different stages of sewer catchments and receiving waters. (ku.dk)
- Cui L, Zhao J, Lu J. Molecular characteristics of extended spectrum beta-lactamase and carbapenemase genes carried by carbapenem-resistant Enterobacter cloacae in a Chinese university hospital. (springermedizin.de)
- In typical circumstances, they derive from genes for TEM-1, TEM-2, or SHV-1 by mutations that alter the amino acid configuration around the active site of these β-lactamases. (wikipedia.org)
- Minimal inhibitory concentration (MIC) of the bacterial strains was determined and genes associated with carbapenemases and extended-spectrum-beta-lactamases (ESBLs) were sequenced and compared with the closest representatives published in public domains. (biomedcentral.com)
- It is, thus, plausible that hospital wastewaters could provide opportunities to find novel carbapenemases and other resistance genes not yet described in clinical strains. (chalmers.se)
- Using a computational hidden Markov model, we detected 27 unique metallo-beta-lactamase (MBL) genes in the shotgun data, of which nine were novel subclass B1 genes, one novel subclass B2, and 10 novel subclass B3 genes. (chalmers.se)
- The study also demonstrates that the microbiota of hospital wastewater can serve as a reservoir of novel resistance genes, including previously uncharacterized carbapenemases with the potential to spread further. (chalmers.se)
- Yet these pathogens have fought back, aided by their membrane organization, which promotes the exclusion and efflux of antibiotics, and by a remarkable propensity to recruit, transfer, and modify the expression of resistance genes, including those for extended-spectrum β-lactamases (ESBLs), carbapenemases, aminoglycoside-blocking 16S rRNA methylases, and even a quinolone-modifying variant of an aminoglycoside-modifying enzyme. (kjim.org)
- NDM beta-lactamases have been a source of particular concern due to their increasing incidence and frequent occurrence within a diverse assortment of mobile genetic elements harboring other resistance genes, leading to extensively drug resistant (XDR) and panresistant (PDR) strains ( 5 - 8 ). (asm.org)
- The aim of this study was to systematically review the published data on the prevalence and dispersion of metallo-β-lactamases (MBLs) genes in A. baumannii in different provinces of Iran and provide an overall prevalence rate using meta-analysis. (eurekaselect.com)
- This test detects the genes encoding OXA-48-like (oxacillin-hydrolyzing beta-lactamase) and VIM (Verona integron-encoded metallo-beta-lactamase) types of beta-lactamases in stool and perirectal/rectal/perianal/anal swabs. (testcatalog.org)
- Carbapenem resistance in K. pneumoniae arises from 2 main mechanisms: permeability defects combined with overexpression of a β-lactamase with weak carbapenemase activity (mostly CTX-M or AmpC cephalosporinases) and the acquisition of carbapenemases ( 2 ). (cdc.gov)
- AmpC mais aucun isolat ne produisait de métallo-bêta-lactamases. (who.int)
- The aim of this study was to determine the prevalence of MBL and AmpC β-lactamases production of K. pneumoniae in clinical specimens of patients referring to Rasool Akram Hospital in Tehran, between July 2016 and December 2017. (alliedacademies.org)
- AmpC β-lactamase, which is a type of cephalosporinase, is known to be responsible for antimicrobial resistance in gram-negative bacilli. (alliedacademies.org)
- AmpC β-lactamases are either plasmid- or chromosomal-mediated [ 10 ]. (alliedacademies.org)
- Worryingly, during the same time period, the over reliance on these antibiotics has already lead to the development of resistance to them (AmpC, Extended-spectrum beta-lactamases and carbapenemases). (microbiologynutsandbolts.co.uk)
- ESBLs are beta-lactamases that hydrolyze extended-spectrum cephalosporins with an oxyimino side chain. (wikipedia.org)
- LABÈGE, France , May 6, 2020 /PRNewswire/ -- Antabio SAS, the biopharmaceutical company focused on developing a broad pipeline of antibacterial treatments against life threatening WHO critical priority pathogens, announced today that the U.S. Food and Drug Administration (FDA) has granted Qualified Infectious Disease Product (QIDP) designation to Antabio's MEM-ANT3310, a combination of meropenem (MEM) and the novel broad-spectrum serine beta-lactamase inhibitor ANT3310. (prnewswire.co.uk)
- ANT3310 is a novel, potent and specific inhibitor of bacterial Serine Beta-Lactamases (SBLs) currently in preclinical development, which displays excellent inhibitory activity against both KPC and OXA carbapenemases. (prnewswire.co.uk)
- Preclinical best-in-class DBO serine ß-lactamase (SBL) inhibitor (ANT3310) to be combined with meropenem for the treatment of KPC- and OXA-producing CRE as well as carbapenem- resistant Acinetobacter baumannii (CRAB), which are widespread globally and for which better broad-spectrum inhibitors are required. (prnewswire.co.uk)
- Production of subclass B1 MBL (carbapenemases) was evaluated by means of the double-disk approximation test using MBL inhibitor, EDTA. (msk.ru)
- Meropenem with vaborbactam ( Vaborem , Menarini) is a combination of a broad-spectrum carbapenem antibiotic, which covers gram-positive, gram-negative and anaerobic bacteria, and a new beta-lactamase inhibitor, which protects against class A and class C (but not class B and class D) carbapenemases (enzymes that cause resistance to carbapenem antibiotics). (nice.org.uk)
- Carbavance is an investigational agent that is a combination of a novel beta-lactamase inhibitor (RPX7009) with a carbapenem for intravenous treatment of hospitalized patients with serious infections. (themedicinescompany.com)
- The Argenta/Antabio collaboration began in February 2013, when the Wellcome Trust awarded Antabio €4.7 Million to fund the discovery of a novel, safe and efficacious pan-inhibitor of bacterial metallo beta-lactamases (MBLs). (technologynetworks.com)
- Antibiotic susceptibility test, screening and phenotypic confirmation for ESBLs and carbapenemases and PCR ( bla CTX-M , bla SHV and bla TEM -type ESBLs, bla VIM , bla IMP and bla NDM-1 -type carbapenemases, and class 1 integron element integrase gene) were performed. (biomedcentral.com)
- Non-repetitive clinical specimens (mid-stream urine, pus, discharge from surgical wound, endotracheal secretions, sputum, catheter tips etc.) received as part of standard patient care investigation from Intensive Care Unit (ICU) and out-patient department (OPD) in Kathmandu Medical College and Teaching Hospital between June 2012 to January 2013 were processed for culture and antibiotic susceptibility testing from patients attending OPD and admitted in ICU were included in the study. (biomedcentral.com)
- Susceptibility data for 2005 and 2007 were collected from 37 and 41 hospitals, respectively, and from one commercial laboratory. (pubmedcentralcanada.ca)
- Vaborbactam cannot protect meropenem against class B and class D beta-lactamases or restore susceptibility when resistance is wholly or partly due to impermeability or efflux mechanisms. (nice.org.uk)
- Suez Canal Uni- Susceptibility testing patients confined to hospital intensive versity hospital is a tertiary-care teaching care units (ICUs) . (who.int)
- Some of these OXA-type carbapenemases are widely dispersed in Pseudomonas aeruginosa and especially in Acinetobacter baumannii. (nih.gov)
- Diversity of carbapenem resistance mechanisms in Acinetobacter baumannii from a Taiwan hospital: spread of plasmid-borne OXA-72 carbapenemase. (nih.gov)
- We investigated the molecular epidemiology of carbapenem-resistant Acinetobacter baumannii from a Taiwanese hospital and determined the mechanisms responsible for resistance. (nih.gov)
- Acinetobacter baumannii (Ab) is a leading cause of hospital infection, mainly in intensive care units. (usp.br)
- In our study, biofilm production and metallo beta-lactamase production were observed among Pseudomonas aeruginosa and Acinetobacter spp. (biomedcentral.com)
- lactamases which include many Gram-negative bacilli, including Acinetobacter spp. (edu.iq)
- This study reports the dissemination of multidrug-resistant (MDR) OXA-23-producing Acinetobacter baumannii clones in hospitals in Antananarivo, Madagascar. (biomedcentral.com)
- On January 13, a urine culture grew an extended spectrum, beta-lactamase-producing Escherichia coli . (cdc.gov)
- Epidemiology and risk factors for isolation of Escherichia coli producing CTX-M-type extended-spectrum β-lactamase in a large U.S. Medical Center. (semanticscholar.org)
- Community-associated extended-spectrum β-lactamase-producing Escherichia coli infection in the United States. (sma.org)
- Acquired carbapenemases in Gram-negative bacterial pathogens: detection and surveillance issues," Clinical Microbiology and Infection , vol. 16, no. 2, pp. 112-122, 2010. (hindawi.com)
- Education programs for physicians and hospital personnel regarding risk reduction for transmission of Enterobacter species and other nosocomial pathogens should be implemented in every hospital. (medscape.com)
- Acquired carbapenemases in Gram-negative bacterial pathogens: detection and surveillance issues. (sciepub.com)
- Phenotypic Detection of carbapenem susceptible metallo-p-lactamase-producing gram negative bacilli in the clinical laboratory. (msjonline.org)
- The Vaborem European public assessment report (EPAR) states that there is an unmet need for well-tolerated antibacterial agents that are active against aerobic gram-negative organisms that express class A and class C carbapenemases. (nice.org.uk)
- Beta-lactamases produced by gram-positive organisms are usually secreted. (wikidoc.org)
- S. Micek, M. T. Johnson, R. Reichley and M. H. Kollef, "An Institutional Perspective on the Impact of Recent Antibiotic Exposure on Length of Stay and Hospital Costs for Patients with Gram-Negative Sepsis," BMC Infectious Diseases, Vol. 12, 2012, p. 56. (scirp.org)
- A positive OXA-48-like (oxacillin-hydrolyzing beta-lactamase) and/or VIM (Verona integron-encoded metallo-beta-lactamase) PCR indicates that the patient is colonized by a Gram-negative bacillus harboring bla OXA-48-like and/or bla VIM, respectively. (testcatalog.org)
- When resistance to broad-spectrum antimicrobials mediated by extended-spectrum β-lactamases (ESBLs) arose in the 1980s, targeted interventions to slow spread were not widely promoted. (cdc.gov)
- Broad-spectrum beta-lactamase production is associated with increased morbidity and mortality in both high- and low/middle-income countries [9, (deepdyve.com)
- The medical director and staff members of the hospital infection control department educated medical and nursing staff members about NDM and needed precautions. (cdc.gov)
- J. Rodríguez-Baño and M. D. Ngugro, "Extended-spectrum β -lactamases in ambulatory care: a clinical perspective," Clinical Microbiology and Infection , vol. 14, no. 1, pp. 104-110, 2008. (hindawi.com)
- Continuous monitoring and surveillance and proper infection control and prevention practices will limit the further spread of these super-bugs within this hospital and beyond. (biomedcentral.com)
- Infection Control Hospital Epidemiol. (msjonline.org)
- These are a major, yet often preventable, threat to patient safety.1 Hospital acquired infection (HAI) as per the World Health Organization(WHO) is defined as "an Infection occurring in a patient in a hospital or other healthcare facility in whom the infection was not present or incubating at the time of admission".2 It also includes an infection acquired during hospital stay and manifesting after discharge. (japi.org)
- Calculated measurements included infection-related mortality, length of hospital stay, and duration of definitive antibiotic therapy. (sma.org)
- A. C. Breier, C. Brandt, D. Sohr, C. Geffers and P. Gastmeier, "Laminar Airflow Ceiling Size: No Impact on Infection Rates Following Hip and Knee Prosthesis," Infection Control and Hospital Epidemiology: The Official Journal of the Society of Hospital Epidemiologists of America, Vol. 32, No. 11, 2011, pp. 1097-1102. (scirp.org)
- They will collect human intrauterine tissue at different time points during pregnancy and during cesarean delivery from hospitals in Brazil and Canada, and investigate the distribution of ABC transporters and the association with infection. (grandchallenges.org)
- There is absolutely no reason to think that if we don't do a good job with infection control that this is going to stay in a hospital, said Hsu. (freerepublic.com)
- A total of 77 patients with confirmed/suspected CRE infection (bacteremia, hospital-acquired/ventilator-associated bacterial pneumonia, complicated intra-abdominal infection, complicated urinary tract infection/acute pyelonephritis) were randomized, and 47 with confirmed CRE infection formed the primary analysis population (microbiologic-CRE-modified intent-to-treat, mCRE-MITT). (springer.com)
- The results of this study may help in tracing and controlling NDM-1-producing K. pneumoniae outbreak by applying effective infection control measures especially in the chest ward of this hospital. (sciepub.com)
- 0:08 Skip to 0 minutes and 8 seconds The epidemiology of extended-spectrum beta-lactamases and carbapenemases can be very complex. (futurelearn.com)
- Although diverse mechanisms were identified, production of OXA-72 carbapenemase was the most common mechanism of carbapenem resistance in A. baumannii from this Taiwanese hospital. (nih.gov)
- In DARWIN, we will undertake a never-previously-performed pan-European examination of the fate of key AMR organisms and genetic determinants in U rban W ater S ystems (UWS) resulting from discharged hospital and community wastes, including transmission mechanisms in different stages of sewer catchments and receiving waters. (ku.dk)
- baumannii multirésistants prélevés dans des unités de soins intensifs d'un grand hôpital de soins de santé tertiaires à Ismaïlia (Égypte), et la fréquence de différentes bêta-lactamases dans ces isolats. (who.int)
- O objetivo do presente trabalho foi avaliar a produção de enzimas carbapenemases do tipo OXA e MβLs, em 36 amostras multirresistentes de A. baumannii , previamente triadas, provenientes de 8 hospitais brasileiros, durante 2004/2008. (usp.br)
- Four of the eight clusters of OXA-type carbapenemases were identified in A. baumannii , including clusters OXA-23, OXA-24, OXA-51, and OXA-58 ( 22 ). (asm.org)
- 1:34 Skip to 1 minute and 34 seconds Regarding carbapenemases, it's important to highlight that they are a biochemically diverse group which includes members of Ambler's class A, B, and C. Reports of its dissemination are increasing worldwide, but they still have distinct geographic niches, which we will now review. (futurelearn.com)
- Wolter D.J., Khalaf N., Robledo I. E., Vázquez G. J., Santé M. I.,Aquino E. E.,Goering R.V., and Hanson N.D. Surveillance of Carbapenem ResistantPseudomonas aeruginosafrom Puerto Rico Medical Center Hospitals: Dissemination of KPC and IMP-18-lactamases. (upr.edu)
- Dissemination of carbapenemases [e.g. (springer.com)
- 23 patients) producing extended spectrum beta-lactamases and/or carbapenemases sourced between 2011 and 2016 in a Portuguese tertiary-care hospital. (cdc.gov)
- A total of 1300 clinical specimens (included 588 burn swabs, 136 wound swabs, 50 from throat, 204 urine, 110 stool, 20 sputum, 162 blood, 15 ears and 15 eyes) were collected from patients in Hillah hospitals over one year period starting from March, 2014 to March, 2015. (edu.iq)
- Colonies which that able role in the colonization and infec- were morphologically consistent with tion of patients admitted to hospitals. (who.int)
- In severely ill patients, this ineffectiveness leads to increased mortality, length of hospital stay, and cost [ 8 , 9 ]. (kjim.org)
- We analyzed antimicrobial resistance data generated by Korean Hospitals and by a commercial laboratory in 2005 and 2007. (pubmedcentralcanada.ca)
- 1 Antimicrobial resistance surveillance serves as a fundamental basis for appreciating trends in resistance, developing accurate treatment guidelines, and evaluating the efficacy of interventions ( www.who.int/drugresistance/en/ ). (pubmedcentralcanada.ca)
- We conducted a retrospective, matched case-control-control, parallel study in a tertiary teaching hospital. (springermedizin.de)
- METHODS: One hundred forty-eight handles and 71 blades deemed ready for patient use from two tertiary hospitals were sampled with sterile swabs using a standardized rolling technique. (bvsalud.org)