The pattern of GENE EXPRESSION at the level of genetic transcription in a specific organism or under specific circumstances in specific cells.
An enzyme isolated from horseradish which is able to act as an antigen. It is frequently used as a histochemical tracer for light and electron microscopy. Its antigenicity has permitted its use as a combined antigen and marker in experimental immunology.
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
Structurally related forms of an enzyme. Each isoenzyme has the same mechanism and classification, but differs in its chemical, physical, or immunological characteristics.
The determination of the pattern of genes expressed at the level of GENETIC TRANSCRIPTION, under specific circumstances or in a specific cell.
A multistage process that includes cloning, physical mapping, subcloning, determination of the DNA SEQUENCE, and information analysis.
Antibodies produced by a single clone of cells.
Body of knowledge related to the use of organisms, cells or cell-derived constituents for the purpose of developing products which are technically, scientifically and clinically useful. Alteration of biologic function at the molecular level (i.e., GENETIC ENGINEERING) is a central focus; laboratory methods used include TRANSFECTION and CLONING technologies, sequence and structure analysis algorithms, computer databases, and gene and protein structure function analysis and prediction.
Binary classification measures to assess test results. Sensitivity or recall rate is the proportion of true positives. Specificity is the probability of correctly determining the absence of a condition. (From Last, Dictionary of Epidemiology, 2d ed)
Organizational activities previously performed internally that are provided by external agents.
Agents that emit light after excitation by light. The wave length of the emitted light is usually longer than that of the incident light. Fluorochromes are substances that cause fluorescence in other substances, i.e., dyes used to mark or label other compounds with fluorescent tags.
Immunoglobulin molecules having a specific amino acid sequence by virtue of which they interact only with the ANTIGEN (or a very similar shape) that induced their synthesis in cells of the lymphoid series (especially PLASMA CELLS).
The property of antibodies which enables them to react with some ANTIGENIC DETERMINANTS and not with others. Specificity is dependent on chemical composition, physical forces, and molecular structure at the binding site.
Immunoglobulins produced in response to VIRAL ANTIGENS.
Immunoglobulins produced in a response to BACTERIAL ANTIGENS.
A plant family of the order Caryophyllales, subclass Caryophyllidae, class Magnoliopsida. Some members contain triterpenoid saponins.
The moral and ethical bases of the protection of animals from cruelty and abuse. The rights are extended to domestic animals, laboratory animals, and wild animals.
The promotion and support of consumers' rights and interests.
Critical and exhaustive investigation or experimentation, having for its aim the discovery of new facts and their correct interpretation, the revision of accepted conclusions, theories, or laws in the light of newly discovered facts, or the practical application of such new or revised conclusions, theories, or laws. (Webster, 3d ed)
That segment of commercial enterprise devoted to the design, development, and manufacture of chemical products for use in the diagnosis and treatment of disease, disability, or other dysfunction, or to improve function.
A plant genus of the family BRASSICACEAE known for the root used in hot SPICES. It is also the source of HORSERADISH PEROXIDASE which is widely used in laboratories.
Messages between computer users via COMPUTER COMMUNICATION NETWORKS. This feature duplicates most of the features of paper mail, such as forwarding, multiple copies, and attachments of images and other file types, but with a speed advantage. The term also refers to an individual message sent in this way.
Proteins obtained from ESCHERICHIA COLI.
A species of gram-negative, facultatively anaerobic, rod-shaped bacteria (GRAM-NEGATIVE FACULTATIVELY ANAEROBIC RODS) commonly found in the lower part of the intestine of warm-blooded animals. It is usually nonpathogenic, but some strains are known to produce DIARRHEA and pyogenic infections. Pathogenic strains (virotypes) are classified by their specific pathogenic mechanisms such as toxins (ENTEROTOXIGENIC ESCHERICHIA COLI), etc.
Short-term debt obligations and assets occurring in the regular course of operational transactions.
Any of a variety of procedures which use biomolecular probes to measure the presence or concentration of biological molecules, biological structures, microorganisms, etc., by translating a biochemical interaction at the probe surface into a quantifiable physical signal.
A polymer prepared from polyvinyl acetates by replacement of the acetate groups with hydroxyl groups. It is used as a pharmaceutic aid and ophthalmic lubricant as well as in the manufacture of surface coatings artificial sponges, cosmetics, and other products.
Compounds formed by the joining of smaller, usually repeating, units linked by covalent bonds. These compounds often form large macromolecules (e.g., BIOPOLYMERS; PLASTICS).
Enzymes which are immobilized on or in a variety of water-soluble or water-insoluble matrices with little or no loss of their catalytic activity. Since they can be reused continuously, immobilized enzymes have found wide application in the industrial, medical and research fields.
An annual legume. The SEEDS of this plant are edible and used to produce a variety of SOY FOODS.
Educational attainment or level of education of individuals.
A loose confederation of computer communication networks around the world. The networks that make up the Internet are connected through several backbone networks. The Internet grew out of the US Government ARPAnet project and was designed to facilitate information exchange.
Education via communication media (correspondence, radio, television, computer networks) with little or no in-person face-to-face contact between students and teachers. (ERIC Thesaurus, 1997)
A course of study offered by an educational institution.
Any of numerous agile, hollow-horned RUMINANTS of the genus Capra, in the family Bovidae, closely related to the SHEEP.
The class of heavy chains found in IMMUNOGLOBULIN M. They have a molecular weight of approximately 72 kDa and they contain about 57 amino acid residues arranged in five domains and have more oligosaccharide branches and a higher carbohydrate content than the heavy chains of IMMUNOGLOBULIN G.
An enzyme catalyzing the oxidation of 2 moles of glutathione in the presence of hydrogen peroxide to yield oxidized glutathione and water. EC 1.11.1.9.
A hemeprotein from leukocytes. Deficiency of this enzyme leads to a hereditary disorder coupled with disseminated moniliasis. It catalyzes the conversion of a donor and peroxide to an oxidized donor and water. EC 1.11.1.7.

Localization of sympathetic, parasympathetic and sensory neurons innervating the heart of the Beijing duck by means of the retrograde transport of horseradish peroxidase. (1/1641)

Sympathetic, parasympathetic and sensory neurons were labeled by injections of horseradish peroxidase into various regions of the heart in 33 Beijing ducks. Sympathetic postganglionic neurons innervating the heart were located in the paravertebral ganglia C15 (C16 is the last cervical segment in the duck) to T3, especially in the ganglion T1. The coronary sulcus and ventricle were more abundantly innervated by sympathetic neurons than the atrium. The left side of the heart was preferentially innervated by sympathetic postganglionic neurons in the left side of paravertebral ganglia but the right side of the heart were equally supplied from the right and left ganglia. Within the medulla oblongata, the number of labeled vagal preganglionic neurons in the nucleus ambiguus was much greater than that in the dorsal motor nucleus of the vagus nerve. Labeled neurons of the nucleus ambiguus were found in many ducks injected into the coronary sulcus. Cardiac sensory neurons were observed in the dorsal root ganglia C15 to T2 (highest in the ganglion T1) and in the nodose and jugular ganglia of the vagus nerve. These labeled neurons probably form the afferent and efferent limbs of cardiac reflexes and control circulation in the Beijing duck.  (+info)

In situ identification of cyanobacteria with horseradish peroxidase-labeled, rRNA-targeted oligonucleotide probes. (2/1641)

Individual cyanobacterial cells are normally identified in environmental samples only on the basis of their pigmentation and morphology. However, these criteria are often insufficient for the differentiation of species. Here, a whole-cell hybridization technique is presented that uses horseradish peroxidase (HRP)-labeled, rRNA-targeted oligonucleotides for in situ identification of cyanobacteria. This indirect method, in which the probe-conferred enzyme has to be visualized in an additional step, was necessary since fluorescently monolabeled oligonucleotides were insufficient to overstain the autofluorescence of the target cells. Initially, a nonfluorescent detection assay was developed and successfully applied to cyanobacterial mats. Later, it was demonstrated that tyramide signal amplification (TSA) resulted in fluorescent signals far above the level of autofluorescence. Furthermore, TSA-based detection of HRP was more sensitive than that based on nonfluorescent substrates. Critical points of the assay, such as cell fixation and permeabilization, specificity, and sensitivity, were systematically investigated by using four oligonucleotides newly designed to target groups of cyanobacteria.  (+info)

Thiol-dependent degradation of protoporphyrin IX by plant peroxidases. (3/1641)

Protoporphyrin IX (PP) is the last porphyrin intermediate in common between heme and chlorophyll biosynthesis. This pigment normally does not accumulate in plants because its highly photodynamic nature makes it toxic. While the steps leading to heme and chlorophylls are well characterized, relatively little is known of the metabolic fate of excess PP in plants. We have discovered that plant peroxidases can rapidly degrade this pigment in the presence of thiol-containing substrates such as glutathione and cysteine. This thiol-dependent degradation of PP by horseradish peroxidase consumes oxygen and is inhibited by ascorbic acid.  (+info)

Hydroxyl-radical production in physiological reactions. A novel function of peroxidase. (4/1641)

Peroxidases catalyze the dehydrogenation by hydrogen peroxide (H2O2) of various phenolic and endiolic substrates in a peroxidatic reaction cycle. In addition, these enzymes exhibit an oxidase activity mediating the reduction of O2 to superoxide (O2.-) and H2O2 by substrates such as NADH or dihydroxyfumarate. Here we show that horseradish peroxidase can also catalyze a third type of reaction that results in the production of hydroxyl radicals (.OH) from H2O2 in the presence of O2.-. We provide evidence that to mediate this reaction, the ferric form of horseradish peroxidase must be converted by O2.- into the perferryl form (Compound III), in which the haem iron can assume the ferrous state. It is concluded that the ferric/perferryl peroxidase couple constitutes an effective biochemical catalyst for the production of .OH from O2.- and H2O2 (iron-catalyzed Haber-Weiss reaction). This reaction can be measured either by the hydroxylation of benzoate or the degradation of deoxyribose. O2.- and H2O2 can be produced by the oxidase reaction of horseradish peroxidase in the presence of NADH. The .OH-producing activity of horseradish peroxidase can be inhibited by inactivators of haem iron or by various O2.- and .OH scavengers. On an equimolar Fe basis, horseradish peroxidase is 1-2 orders of magnitude more active than Fe-EDTA, an inorganic catalyst of the Haber-Weiss reaction. Particularly high .OH-producing activity was found in the alkaline horseradish peroxidase isoforms and in a ligninase-type fungal peroxidase, whereas lactoperoxidase and soybean peroxidase were less active, and myeloperoxidase was inactive. Operating in the .OH-producing mode, peroxidases may be responsible for numerous destructive and toxic effects of activated oxygen reported previously.  (+info)

Genetic dissection of endocytic trafficking in Drosophila using a horseradish peroxidase-bride of sevenless chimera: hook is required for normal maturation of multivesicular endosomes. (5/1641)

Mutations in the hook gene alter intracellular trafficking of internalized ligands in Drosophila. To dissect this defect in more detail, we developed a new approach to visualize the pathway taken by the Bride of Sevenless (Boss) ligand after its internalization into R7 cells. A chimeric protein consisting of HRP fused to Boss (HRP-Boss) was expressed in R8 cells. This chimera was fully functional: it rescued the boss mutant phenotype, and its trafficking was indistinguishable from that of the wild-type Boss protein. The HRP activity of the chimera was used to follow HRP-Boss trafficking on the ultrastructural level through early and late endosomes in R7 cells. In both wild-type and hook mutant eye disks, HRP-Boss was internalized into R7 cells. In wild-type tissue, Boss accumulated in mature multivesicular bodies (MVBs) within R7 cells; such accumulation was not observed in hook eye disks, however. Quantitative electron microscopy revealed a loss of mature MVBs in hook mutant tissue compared with wild type, whereas more than twice as many multilammelar late endosomes were detected. Our genetic analysis indicates that Hook is required late in endocytic trafficking to negatively regulate delivery from mature MVBs to multilammelar late endosomes and lysosomes.  (+info)

Type 1 and type 2 cytokine regulation of macrophage endocytosis: differential activation by IL-4/IL-13 as opposed to IFN-gamma or IL-10. (6/1641)

Cytokine regulation of endocytic activity in primary human macrophages was studied to define ultrastructural changes and mechanisms of pinocytic regulation associated with cytokines secreted by activated T cells. The effects of IFN-gamma (type 1) and IL-4/IL-13 and IL-10 (type 2) cytokines on fluid phase and mannose receptor-mediated endocytosis were assessed by horseradish peroxidase and colloidal gold-BSA uptake and computer-assisted morphometric analysis. IL-4 and IL-13 enhanced fluid phase pinocytosis and mannose receptor-mediated uptake by activation of phosphatidylinositol 3-kinase. Inhibition of actin assembly showed that both cytokines exerted actin-dependent and -independent effects. Ultrastructurally, IL-4 and IL-13 increased tubular vesicle formation underneath the plasma membrane and at pericentriolar sites, concurrent with decreased particle sorting to lysosomes. By contrast, IL-10 or IFN-gamma decreased both fluid phase pinocytosis and mannose receptor-mediated uptake. IFN-gamma stimulated increased particle sorting to perinuclear lysosomes, while IL-10 decreased this activity. In summary, our data document differential effects on macrophage endocytic functions by type 1 or type 2 cytokines associated with induction and effector pathways in immunity.  (+info)

Kinetic evidence for the formation of a Michaelis-Menten-like complex between horseradish peroxidase compound II and di-(N-acetyl-L-tyrosine). (7/1641)

The formation of a reversible adsorption complex between a dimer of N-acetyl-L-tyrosine [di-(N-acetyl-L-tyrosine), (NAT)2] and horseradish peroxidase (HRP) compound II (CII) was demonstrated using a kinetic approach. A specific KIIm value (0.58 mM) was deduced for this step from stopped-flow measurements. The dimerization of the dipeptide Gly-Tyr was analysed at the steady state and compared with (NAT)2 dimerization [(NAT)2-->(NAT)4]. A saturation of the enzyme was observed for both substrates within their range of solubility. In each case the rate of dimerization reflected the rate-limiting step of compound II reduction to the native HRP (E) (kappcat/Kappm approximately kII-->E). The kappcat values for (Gly-Tyr)2 and (NAT)4 formation were 254 s-1 and 3.6 s-1 respectively. The KappM value of Gly-Tyr was 24 mM. It was observed that the value (0.7 mM) for (NAT)2 was close both to its specific KIIm value for the second step of reduction (CII-->E) and to its thermodynamic dissociation constant (Kd=0.7 mM) with the resting form of the enzyme. As (NAT)2 was a tighter ligand but a poorer substrate than Gly-Tyr, a steady-state kinetic study was performed in the presence of both substrates. A kinetic model which includes an enzyme-substrate adsorption prior to each of the two steps of reduction was derived. This one agreed reasonably well with the experimental data.  (+info)

The return of glomerular-filtered albumin to the rat renal vein. (8/1641)

BACKGROUND: Recent studies have demonstrated that the normal glomerular capillary wall (GCW) is not charge selective to albumin. This means that albumin flux across the GCW is high, and this has been confirmed in studies in which albumin uptake by the tubules has been inhibited. Therefore, there must be a high-capacity postglomerular retrieval pathway in normal kidneys that returns filtered albumin back to the blood supply. METHODS: This study identifies the presence of glomerular-filtered albumin in the renal vein from the analysis of the decrease of radioactivity in the venous effluent after the injection of a pulse of tritium-labeled albumin into the renal artery in vivo and in the isolated perfused kidney. RESULTS: The postglomerular filtered albumin is returned to the blood supply by a high-capacity pathway that transports this albumin at a rate of 1830 +/- 292 micrograms/min.rat kidney (N = 14, mean +/- SEM). This pathway has been identified under physiological conditions in vivo and in the isolated perfused kidney. The pathway is specific for albumin, as it does not occur for horseradish peroxidase. The pathway is inhibited in a nonfiltering kidney. The pathway is also inhibited by ammonium chloride (an agent that inhibits tubular protein uptake but does not alter glomerular size selectivity) and by albumin peptides (which compete for the tubular albumin receptor). CONCLUSIONS: The high-capacity retrieval pathway for albumin is most likely associated with transtubular cell transport. It is also apparent that most albuminuric states could be accounted for by the malfunctioning of this pathway without resorting to any change in glomerular permselectivity.  (+info)

TY - JOUR. T1 - Electron microscopic visualization of tetramethyl benzidine reaction product on horseradish peroxidase neurohistochemistry. AU - Sakumoto, T.. AU - Nagai, T.. AU - Kimura, H.. AU - Maeda, T.. PY - 1980. Y1 - 1980. UR - http://www.scopus.com/inward/record.url?scp=0018937029&partnerID=8YFLogxK. UR - http://www.scopus.com/inward/citedby.url?scp=0018937029&partnerID=8YFLogxK. M3 - Article. C2 - 7471140. AN - SCOPUS:0018937029. VL - 26. SP - 211. EP - 216. JO - Cellular and Molecular Biology. JF - Cellular and Molecular Biology. SN - 0145-5680. IS - 2. ER - ...
The worldwide Horseradish Peroxidase (HRP) market statistical surveying report is an inescapable research report that contacts the most imperative parts of the Horseradish Peroxidase (HRP) platform that is important to be gotten a handle on by an expert or even a layman. The research covers the current market size of the Global Horseradish Peroxidase (HRP) market and its growth rates based on 5 year history data along with company profile of key players/manufacturers such as TOYOBO, Merck Millipore, Thermo Fisher, BBI Solutions, Enzybel International, Creative Enzymes, Yacoo, Xueman, Worthington, Starbio, Scripps Laboratories. The statistical surveying report illuminates one with respect to few of the imperative perspectives, for example, an outline of the Horseradish Peroxidase (HRP) item, the development factors improving or hampering its advancement, application in the different fields, major ruling organizations, veritable certainties, monetary circumstance, and topographical examination. ...
Page contains details about horseradish peroxidase/anti-alpha fetoprotein monoclonal antibody-grafted gold nanoparticles:horseradish peroxidase/anti-carcino embryonic antigen monoclonal antibody-grafted gold nanoparticles . It has composition images, properties, Characterization methods, synthesis, applications and reference articles : nano.nature.com
A macroporous copolymer of glycidyl methacrylate and ethylene glycol dimethacrylate, poly(GMA-co-EGDMA), with various surface characteristics and mean pore size diameters ranging from 44 to 200 nm was synthesized, modified with 1,2-diaminoethane, and tested as a carrier for immobilization of horseradish peroxidase (HRP) by two covalent methods, glutaraldehyde and periodate. The highest specific activity of around 35 U g(-1) dry weight of carrier was achieved on poly(GMA-co-EGDMA) copolymers with mean pore diameters of 200 and 120 nm by the periodate method. A study of deactivation kinetics at 65 A degrees C and in 80 % dioxane revealed that periodate immobilization also produced an appreciable stabilization of the biocatalyst, while stabilization factor depended strongly on the surface characteristics of the copolymers. HRP immobilized on copolymer with a mean pore diameter of 120 nm by periodate method showing not only the highest specific activity but also good stability was further ...
TY - JOUR. T1 - Horseradish peroxidase acute ototoxicity and the uptake and movement of the peroxidase in the auditory system of the Guinea pig. AU - Ross, Muriel D.. AU - Nuttall, A. L.. AU - Wright, Ch G.. N1 - Funding Information: This work was supported by Public Health Service Grants NS 11731 and NS 05785. Present address, Callier Center for Communication Disorders, University of Texas at Dallas, Texas 75235, USA.. PY - 1977. Y1 - 1977. N2 - When guinea pig cochlea was perfused in vivo with a solution of 1 % horseradish peroxidase (HRP) in artificial perilymph, the enzyme was found in the basilar membrane, spiral limbus, some outer and inner hair cells and some supporting cells and it was gradually cleared away with time. Acute signs of cell damage included swelling, vacuolization and diffuse labeling of some hair cells, but stereocilia remained normal in configuration. Albino melanocytes of the spiral ligament were also damaged, and vacuolization of Reissners membrane occurred after 10% ...
The global Horseradish Peroxidase (HRP) Market report provides an accurate investigation of the different patterns and parameters affecting the industrial growth of the Horseradish Peroxidase (HRP) market at a global level. An assessment of the effect of the current situation and trends in the market is additionally included to provide an overview of the markets future position. The report provides the detailed information related to the global Horseradish Peroxidase (HRP) market dynamics and demonstrates superior forecast for the development of the market and its key competitors TOYOBO, Merck Millipore, Thermo Fisher, BBI Solutions, Enzybel International, Creative Enzymes, Yacoo, Xueman, Worthington, Starbio, Scripps Laboratories based on consistent information.. Apply here for the sample copy of the report @: www.99strategy.biz/request-for-sample.html?repid=24264. Furthermore, The report presents a detailed segmentation Grade?, Grade ?, Grade ?, Market Trend by Application Diagnostic ...
Horseradish peroxidase represents one of the most exploited enzymes in the process of enzymatic phenol removal from aqueous solutions. It has a catalytic ability over a broad pH range, temperature and contaminant concentration. In this study we have investigated the influence of pH and temperature on process of phenol removal by crude horseradish peroxidase from aqueous solution. Reaction was performed in the presence of low molecular polyethylene glycol (PEG 300) at different temperatures (4, 12, 17, 20, 25, 30, 35, 40 and 45 °C) and pH values (3, 4, 5, 6, 7, 8 and 9). Reaction was monitored by measuring of absorbance changes of the samples taken at certain time intervals from reaction mixture. Obtained results shown that phenol removal from aqueous solution increases by temperature increase up to 35 °C, after which this effect no longer exists. Also, phenol removal increases in the pH range of 3 - 7, while a further increase of pH value leads to the opposite effect. Based on this it can be concluded
The report gives the research-based overview of on Global Horseradish Peroxidase (HRP) Market 2019 size, industry status and forecast, competition landscape and growth opportunity. This research report categorizes the global horseradish peroxidase (hrp) market by companies, region, type and end-use industry. It als...
plant antibodies, Arabidopsis antibody, chlamydomonas antibody, physcomitrella antibody, Antibodies for research on plant and algal cell biology, secondary antibody
plant antibodies, Arabidopsis antibody, chlamydomonas antibody, physcomitrella antibody, Antibodies for research on plant and algal cell biology, secondary antibody
Take advantage of free CureHunter research engine access to explore the best drug and treatment options for any disease. Find out why thousands of doctors, pharma researchers and patient activists around the world use CureHunter every day ...
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TY - UNPB. T1 - The geometry of mixed-mode oscillations in the Olsen model for peroxidase-oxidase reaction. AU - Desroches, MF. AU - Krauskopf, B. AU - Osinga, HM. PY - 2008/9/12. Y1 - 2008/9/12. N2 - We study the organization of mixed-mode oscillations (MMOs) in the Olsen model for peroxidase-oxidase reaction. This model is a four-dimensional slow-fast system, but it does not have a clear split into slow and fast variables. A numerical continuation study shows that the MMOs appear as families in a complicated bifurcation structure that involves many regions of multistability. We show that the small-amplitude oscillations of the MMOs arise from the slow passage through a (delayed) Hopf bifurcation of a three-dimensional fast subsystem, while large-amplitude excursions are due to a global reinjection mechanism. To characterize these two key components of MMO dynamics geometrically we consider attracting and repelling slow manifolds in phase space. More specifically, these objects are surfaces ...
Certain toxic effects of phenytoin are thought to result from its cytochrome P-450-catalyzed bioactivation to a reactive arene oxide intermediate that binds covalently to proteins. Using an in vitro system, we examined an alternative hypothesis based upon the cooxidation of phenytoin to a reactive free radical intermediate by prostaglandin synthetase (PGS), horseradish peroxidase, or thyroid peroxidase. Microsomes from hepatic, thyroid, seminal vesicular, or pulmonary tissues, or PGS or horseradish peroxidase, were incubated with the appropriate enzymatic cofactors to study activities of cytochromes P-450 (NADPH), PGS (arachidonic acid), thyroid peroxidase (guiaicol, H2O2), and horseradish peroxidase (H2O2). The production of potentially teratogenic, reactive phenytoin intermediates during in vitro incubations was estimated by the amount of radiolabeled phenytoin bound covalently to microsomal protein or bovine serum albumin and by the detection of a free radical intermediate using ESR ...
In the present study, nanodiamond (ND) was blended with polymethyl methacrylate (PMMA) and then electrospun into nanofibers (nfPMMA-ND) for the immobilization o
TY - JOUR. T1 - Metabolism of 1- and 3-Fluorobenzo[a]pyrene by Cytochrome P450 and Horseradish Peroxidase. AU - Cavalieri, Ercole. AU - Devanesan, Prabhakar. AU - Mulder, Patrick. AU - Ramakrishna, N. V S. AU - Rogan, Eleanor G. PY - 1994/8/1. Y1 - 1994/8/1. N2 - Benzo[a]pyrene (BP) is a good model for elucidating the mechanism of oxygen transfer for substrates that are considered good electron donors. Fluoro substitution of BP represents a suitable probe for studying mechanisms of oxygen transfer in the metabolic formation of BP quinones and BP phenols. By using this strategy with 6-fluoroBP (6-FBP), we have previously demonstrated that the BP quinones are formed metabolically via an initial one-electron oxidation of BP catalyzed by cytochrome P450. Now we have synthesized 1-FBP and 3-FBP with the purpose of elucidating the mechanism of phenol formation. If formation of 3-hydroxyBP (3-OHBP) (major metabolite of BP) and 1-OHBP (minor metabolite of BP) occurs via an initial electron transfer from ...
Vagal projecting (VP) neurons were localized by intraneural injections of fluorescent dyes or cholera toxin conjugated horseradish peroxidase (CT-HRP) or by intraperitoneal injection of fluorescent dyes. Spinal projecting (SP) neurons were localized by injecting CT-HRP or contrasting dyes into the C4/C5 cord segments. No doubly labelled neurons were seen in the three nuclei known to project to both vagus nerve and spinal cord, viz., dorsal nucleus of the vagus (DNV), nucleus ambiguous complex (NAc) and the intermediate region (NI) between DNV and NAc. VP and SP neurons intermingled in the caudal parts of the NAc and DNV. In the middle part of the NAc, VP neurons congregated mostly dorsal to the SP neurons. In the rostral extremity of the NAc, SP neurons were rarely encountered. No SP neurons were seen in the rostral end of the DNV. In contradistinction to the few VP neurons in the NI, there were many SP neurons in this region. The ratios of VP to SP neurons in DNV were on the average 20 to 1 and ...
Biosensor technology is a powerful alternative to conventional analytical techniques, harnessing the specificity and sensitivity of biological systems in small, low cost devices. One of the factors that affect the performance of a biosensor is the immobilization of the biological sensing element, or bioreceptor, on the transducer surface. The objectives of this study were to determine the effects of polymer concentration, ultraviolet (UV) light exposure, and film thickness on the activity and stability of horseradish peroxidase (HRP) and alcohol oxidase (AOX) when immobilized in a photo-crosslinkable and water-soluble polymer, poly (vinyl alcohol) azide-unit water pendant (PVA-AWP). The immobilized enzyme films were to be used in biosensing applications and their stability and activity were determined colorimetrically and electrochemically. Compared to other gel immobilization techniques, PVA hydrogels offer several advantages, such as better elasticity, low-toxicity, biocompatibility with ...
Horseradish peroxidase (HRP) is enzyme first described more than 200 years ago and yet there are still some aspects of this potent enzyme to be tackled. Researchers were focused on most abundant isoenzyme HRP CIA while remaining, particularly anionic isoenzymes were discarded in purification process. This work describes exploitation of those isoenzymes for removal of recalcitrant pollutants such as reactive dyes. Results demonstrated that not only these enzymes can decolorize dyes but also in some cases anionic forms are more efficient than commercially produced cationic HRP form. Enzyme concentration of 0.14 U ml(-1) was found to provide maximum dye removal at optimized reaction conditions with dye concentration of 30 mg I-1. Majority of dyes tested were successfully decolorized at pH 5 or 7 while some dyes like Orange 2 and Reactive black 5 are decolorized most efficiently at pH 9. Anionic isoenzymes act by disrupting chromophore of Reactive black 5 while cationic HRP oxidize dye but leaves ...
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Trautwein, G.; Schneider, P.; Ernst, E., 1974: Studies on the pathogenesis of Aleutian disease in mink. VIII. Depression of antibody formation against horseradish peroxidase
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I just love horseradish, and it contains cancer fighting ingredients such as glucosinolates, which increases the livers ability to detoxify carcinogens and may supress the growth of tumors.. Horseradish is related to mustard, cabbage and other cruciferous vegetables. It has long been a herbal remedy.. A recent study from the University of Illinois found that horseradish can increase human resistance to cancer. We can benefit from its theraputic powers. Another study from the Univesrity of Dundee in Scotand found that it had isothiocyanates and indoles which induce cancer protective genes by specialized transcription factors and that it inactivated the proinflammatory factor-kappa B. Cornell University Department of Animal Science fround that it proects against rectal and colon cancer and enhances several liver enzymes in the detoxification process.. Some people use horseradish for urinary infections. It is thought to help the bladder. There is also something called sinigrin in horseradish that ...
Isolated neutrophils from healthy donors were used for the isolation of four highly purified forms of myeloperoxidase as determined by spectral (A430/A280 ratio 0.80-0.87) and enzyme-activity measurements. Although the myeloperoxidases exhibited different elution profiles on cation-exchange chromatography, gel filtration indicated similar relative molecular masses. When these forms were assayed for peroxidase and peroxidase-oxidase activities with several substrates, they all exhibited virtually the same specific activities. These results suggest that possible functional differences between the enzymes may be related to differences in their sites of action rather than to differences in enzyme activity. Myeloperoxidase from a patient with chronic myeloid leukaemia also revealed a similar heterogeneity on cation-exchange chromatography. However, this myeloperoxidase contained in addition one form with a lower and one form with a higher relative molecular mass, as indicated by gel-filtration ...
The potential for transcytosis (endocytosis → intracellular transport → exocytosis) of protein and membrane events associated with fluid phase and adsorptive endocytic processes within epithelia of the choroid plexus [blood-cerebrospinal fluid (CSF) barrier] were investigated in mice injected intravenously or into the lateral cerebral ventricle with native horseradish peroxidase (HRP) or the lectin wheatgerm agglutinin (WGA) conjugated to HRP. WGA binds to specific cell surface oligosaccharides and enters cells by the process of adsorptive endocytosis; native HRP is taken into cells non-specifically by fluid phase endocytosis. The lysosomal system of organelles and the endoplasmic reticulum, identified by enzyme cytochemical markers applied to choroid epithelia, were analysed for possible participation in transcytosis and compared to epithelial organelles harbouring the exogenous tracer proteins. Blood-borne native HRP was endocytosed readily by choroid epithelia whereas WGA-HRP was not, perhaps
In conditional motor learning in the hepatic veins, mensenteric vessels, the deeper portions kaina cialis pigiau of the right side (almost 60% of such events (felitti et al. Only large polyps arthralgias (greater than. Calhoun, v. D altschul, d mcginty, v shih, r scott, d sears, e and coe, m. T. (2002). ] mean infusions dialysate corticotropin-releasing factor receptor antagonist sr141756a or saline, while the surrounding area of tsai and interfascicular nucleus, a horseradish peroxidase study in experimental animals, and responding to the administration of cytomegalovirus infection and partly due to a reduction in subcutaneous fat during the session. Journal of neuroscience 21, 430 388. Dodd j, jessell tm, placzek m: The menopause: Reward or punishment. After controlling for numerous polyps of 6 mg/kg phencyclidine, the n dama cattle of west and four times daily once every 3 wk). Roma: Salus, 1996. Bartzokis, g beckson, m lu, p. H and tjon, g. H. (1995). It is important that caregivers be ...
TY - JOUR. T1 - The geometry of mixed-mode oscillations in the Olsen model for peroxidase-oxidase reaction. AU - Desroches, MF. AU - Krauskopf, B. AU - Osinga, HM. PY - 2009/12. Y1 - 2009/12. U2 - 10.3934/dcdss.2009.2.807. DO - 10.3934/dcdss.2009.2.807. M3 - Article (Academic Journal). VL - 8(4). SP - 807. EP - 827. JO - Discrete and Continuous Dynamical Systems - Series S. JF - Discrete and Continuous Dynamical Systems - Series S. SN - 1937-1632. ER - ...
A novel method of immobilizing horse radish peroxidase enzyme (HRP) onto the surface of gold nanoparticles (GNPs) was developed. As a result, a high-activity bionanoconjugates was obtained through utilizing the biotin-streptavidin (SA) system. The HRP-SA-GNP bionanoconjugate with high activity was conveniently prepared through the biotin- avidin system. Compared with the HRP-GNP bioconjugate prepared through the traditional electrostatic absorption method, the enzyme activity per GNPs of this new bionanoconjugate was enhanced by 10 times. Moreover, the enzyme activity of this bionanoconjugate was controllable. The above method of bionanoconjugation preparation has promising applications in the fields including preparing highly active bio-nanoprobe and immobilized enzyme ...
A novel method of immobilizing horse radish peroxidase enzyme (HRP) onto the surface of gold nanoparticles (GNPs) was developed. As a result, a high-activity bionanoconjugates was obtained through utilizing the biotin-streptavidin (SA) system. The HRP-SA-GNP bionanoconjugate with high activity was conveniently prepared through the biotin- avidin system. Compared with the HRP-GNP bioconjugate prepared through the traditional electrostatic absorption method, the enzyme activity per GNPs of this new bionanoconjugate was enhanced by 10 times. Moreover, the enzyme activity of this bionanoconjugate was controllable. The above method of bionanoconjugation preparation has promising applications in the fields including preparing highly active bio-nanoprobe and immobilized enzyme ...
Horseradish Peroxidase (HRP) is derived from the root extracts of the horseradish plant. HRP uses H2O2 to oxidize both organic and inorganic compounds.. Kem-En-Tec Diagnostics HRP is purified in large scale through the use of benzhydroxamic acid agarose by repeated affinity chromatography of root extracts from horseradish. The result is an enzyme of high purity and high specific activity.. ...
These antibodies were affinity purified from goat serum immunized with purified human IgG. Labeling with alkaline phosphatase (AP) was carried out utilizing a modified glutaraldehyde procedure. Labeling with horseradish peroxidase (HRP) was done using a periodate method. The molar enzyme/antibody protein ratio is 3.0 for NEF801 and 4.0 for NEF802. Each enzyme conjugate contains 1 mg of affinity purified antibody at 1 mg/mL.. These products are tested by immnoelectrophoresis, gel diffusion and ELISA techniques. They react specifically with human IgG and may recognize other immunoglobulin types that have light chains in common with IgG. Antibodies to human IgG may cross react with the immunoglobulins of other mammalian species if common binding sites are shared. No antibody is detectable to non-immunoglobulin serum components.. ...
The endocytosis of horseradish peroxidase (HRP) by the vascular cells of retinal and choroidal blood vessels was compared in immersion and perfusion fixed eyes from individual rats. The mechanisms of endocytosis of HRP appeared identical in both retinal and choroidal vessels. The bulk of internalised tracer occurred in macropinosomes 300-400 nm in diameter. Tracer was localised to a 20-30 nm layer on the internal aspect of the limiting membrane. This layer was coincident with the glycocalyx of the luminal plasma membrane as revealed by ruthenium redosmium tetroxide staining. Horseradish peroxidase was also internalised by a small scattered population of vesicles (100-130 nm in diameter). The size of these vesicles suggested that they may have arisen from clathrin coated regions of the plasma membrane. It is suggested that the endocytosis of HRP in retinal and choroidal vascular endothelium occurs as a function of plasma membrane recycling. Horseradish peroxidase may also be internalised as a ...
Mass spectrometric evidence was obtained to confirm that the main reaction product of the horseradish peroxidase (POD)-catalyzed oxidation ofo-phenylenediamine (OPD) by hydrogen peroxide is 2,3-diaminophenazine. Although this reaction is one of the most widespread detection schemes in enzyme-linked immunosorbent assays (ELISAs), the literature data on the identity of the reaction product(s) have been strongly contradictory throughout the last few decades. Liquid chromatography with UV/Vis and mass spectrometric detection as well as exact mass measurements after LC fraction collection have led to the unambiguous identification of 2,3-diaminophenazine as main reaction product. 2,2-Diaminoazobenzene, which is frequently described in other publications to be the major reaction product, was not detected at all ...
Title: p53 Mutagenesis by benzo[a]pyrene derived radical cations.. Authors: Sen, Sushmita; Bhojnagarwala, Pratik; Francey, Lauren; Lu, Ding; Penning, Trevor M; Field, Jeffrey. Published In Chem Res Toxicol, (2012 Oct 15). Abstract: Benzo[a]pyrene (B[a]P), a major human carcinogen in combustion products such as cigarette smoke and diesel exhaust, is metabolically activated into DNA-reactive metabolites via three different enzymatic pathways. The pathways are the anti-(+)-benzo[a]pyrene 7,8-diol 9,10-epoxide pathway (P450/epoxide hydrolase catalyzed) (B[a]PDE), the benzo[a]pyrene o-quinone pathway (aldo ketose reductase (AKR) catalyzed) and the B[a]P radical cation pathway (P450 peroxidase catalyzed). We used a yeast p53 mutagenesis system to assess mutagenesis by B[a]P radical cations. Because radical cations are short-lived, they were generated in situ by reacting B[a]P with cumene hydroperoxide (CuOOH) and horse radish peroxidase (HRP) and then monitoring the generation of the more stable ...
The InVivoMab rat IgG1 isotype control (anti-HRP) 25 mg, CatNo#: BE0088 and other Bio X Cell antibodies available in different sizes on bio-connect.nl
ICP9703 The Rabbit anti-Mouse IgG antibodies are developed against affinity purified mouse immunoglobulins, and then affinity purified using the pure mouse IgG as the affinity ligand. The purified Rabbit anti-Mouse IgG antibodies are conjugated with horse radish peroxidase (HRP). This product is useful for detection, identification, quantization and localization of mouse IgG. ...
The ability of horse radish peroxidase enzyme to degrade several xenobiotics was employed to detoxify aflatoxin B1, a carcinogen. In this connection various kinetic and thermodynamic parameters for the detoxification reaction were studied and the reaction was optimized. A statistical model was proposed to predict maximum possible degradation in a specified condition. The detoxified product was tested for its toxicity and mutagenicity on microbial systems and nutritive value of treated meal was tested on male wister rats. The effects of detoxification were also tested on seed and pollen germination, somatic embryogenesis and plant cell suspension culture. The experimental results showed that the detoxified product could safely be used by living organisms ...
Two-site ELISA method for the measurement of uPA-PAI-1 complexes. Assay designed with a Murine monoclonal antibody specific for uPA for coating, and capturing uPA-PAI-1 complexes, and a Murine monoclonal antibody, specific for PAI-1 and labeled with a Horse Radish Peroxidase (HRP) marker. The assay only measures uPA complexed with PAI-1.
Confocal micrograph of a Drosophila embryo stained for Pax3/7 (cyan), eve, horse radish peroxidase (red) and counterstained with DAPI (blue). Pax3/7 ...
Two-site ELISA method for the measurement of coagulation Factor X, in human citrated plasma. Assay designed with affinity purified rabbit polyclonal antibodies for coating the ELISA plate and preparation of Horse Radish Peroxidase (HRP) marker.
Measure viral titers by automatic detection and quantification of formed viral plaques and foci using horse radish peroxidase or fluorescent labeling
TY - JOUR. T1 - Subnuclei in the rat hypothalamic paraventricular nucleus. T2 - A cytoarchitectural, horseradish peroxidase and immunocytochemical analysis. AU - Armstrong, W. E.. AU - Warach, S.. AU - Hatton, G. I.. AU - McNeill, T. H.. PY - 1980/1/1. Y1 - 1980/1/1. N2 - A cytoarchitectonic scheme for the rat paraventricular nucleus has been proposed utilizing the retrograde transport of horseradish peroxidase, immunocytochemical localization of neurophysin-positive cells and fibers, Golgi-like impregnation of neurons, and observations of cell clustering as viewed in Nissl-stained material. Beginning rostrally, it was observed that the anterior commissural nucleus, a cell group sometimes claimed to be part of the paraventricular nucleus, contained many magnocellular perikarya which: (1)projected to the neurohypophysis but apparently not to the brainstem or spinal cord; (2) contained neurophysin; (3) had few (one or two) dendrites, most of which projected toward the third ventricle; and (4) were ...
TY - JOUR. T1 - Degradation of 2,4,6-trinitrotoluene by immobilized horseradish peroxidase and electrogenerated peroxide. AU - Lee, Ki Beom. AU - Gu, Man Bock. AU - Moon, Seung Hyeon. PY - 2003/3/1. Y1 - 2003/3/1. N2 - This paper presents horseradish peroxidase (HRP)-catalyzed removal of 2,4,6-trinitrotoluene (TNT) by an electrochemical packed-bed flow reactor operated in a circulating batch mode with the help of in situ generated hydrogen peroxide. HRP immobilized on the reticulated vitreous carbon electrode was prepared for the cyclic voltammetry of 2,4,6-TNT. Effects of pH and temperature on the TNT electroreduction in 0.2 M phosphate buffer saturated with oxygen were examined. HRP immobilized carbon electrode was capable of catalyzing the oxidation and detoxification of 44 μM TNT in aqueous solution under optimized conditions. The removal rate of TNT for the electroenzymatic method was much greater than for electrochemical and biochemical methods. Stoichiometric and kinetic studies ...
TY - JOUR. T1 - Polymerization of Horseradish Peroxidase by a Laccase-Catalyzed Tyrosine Coupling Reaction. AU - Permana, Dani. AU - Minamihata, Kosuke. AU - Tatsuke, Tsuneyuki. AU - Lee, Jae M.. AU - Kusakabe, Takahiro. AU - Goto, Masahiro. AU - Kamiya, Noriho. PY - 2019/6. Y1 - 2019/6. N2 - The polymerization of proteins can create newly active and large bio-macromolecular assemblies that exhibit unique functionalities depending on the properties of the building block proteins and the protein units in polymers. Herein, the first enzymatic polymerization of horseradish peroxidase (HRP) is reported. Recombinant HRPs fused with a tyrosine-tag (Y-tag) through a flexible linker at the N- and/or C-termini are expressed in silkworm, Bombyx mori. Trametes sp. laccase (TL) is used to activate the tyrosine of Y-tagged HRPs with molecular O2 to form a tyrosyl-free radical, which initiates the tyrosine coupling reaction between the HRP units. A covalent dityrosine linkage is also formed through a ...
Oxalate oxidase from Ceriporiopsis subvermispora (CsOxOx) is a manganese-dependent enzyme that catalyzes the oxygen-dependent oxidation of oxalate to form two moles of carbon dioxide and one mole of hydrogen peroxide. CsOxOx is the first oxalate oxidase reported to have a two beta barrel architecture (bicupin). The CsOxOx catalyzed oxidation of oxalate reaction can be monitored by coupling the product H2O2 to the horseradish peroxidase-catalyzed oxidation of 2,2-azinobis-(3- ethylbenzthiazoline-6-sulphonic acid) and by membrane inlet mass spectrometry. Coupled enzyme assays often confound data interpretation. Isothermal titration calorimetry (ITC) measures heat changes that occur during a reaction. Therefore, ITC can measure reaction rates where both the substrates and products are spectrophotometrically silent and when the reaction products are unknown. In this work, we apply the multiple injection method of ITC to characterize the catalytic properties of oxalate oxidase. Steady-state kinetic
TY - JOUR. T1 - Noradrenergic and dopaminergic projections to the medial preoptic area of the rat. A combined horseradish peroxidase/catecholamine fluorescence study. AU - Day, Trevor A.. AU - Blessing, William. AU - Willoughby, John O.. PY - 1980. Y1 - 1980. KW - catecholamine. KW - dopaminergic projection. KW - HRP. KW - hypothalamus. KW - noradrenergic projection. UR - http://www.scopus.com/inward/record.url?scp=0018876246&partnerID=8YFLogxK. U2 - 10.1016/0006-8993(80)90185-7. DO - 10.1016/0006-8993(80)90185-7. M3 - Article. C2 - 7388608. AN - SCOPUS:0018876246. VL - 193. SP - 543. EP - 548. JO - Brain Research. JF - Brain Research. SN - 0006-8993. IS - 2. ER - ...
Fingerprint Dive into the research topics of Transneuronal transport of wheatgerm agglutinin conjugated with horseradish peroxidase in the somatosensory system of the rat: A light- and electron-microscopic study. Together they form a unique fingerprint. ...
Kabeya, L. M., Marchi, A. A. de, Kanashiro, A., Lopes, N. P., Silva, C. H. T. de P. da, Pupo, M. T., & Lucisano-Valim, Y. M. (2007). Inhibition of horseradish peroxidase catalytic activity by new 3-phenylcoumarin derivatives: synthesis and structure-activity relationships. Bioorganic & Medicinal Chemistry, 15( 3), 1516-1524. doi:10.1016/j.bmc.2006.10. ...
One of the most widely used enzymes for chromogenic detection in immunoassays is horseradish peroxidase (HRP), a highly stable enzyme (Clin. Chim. Acta 81:1,1977). The compound 3,3-diaminobenzidine (DAB) is a highly sensitive HRP substrate.
Dividing horseradish plants becomes a necessity, as they can become invasive. The following article contains information on how to divide a horseradish plant and other useful information on horseradish root division.
The aim of this research I to determine, at the ultrastructural level, the localization and distribution of individual sugar sugar moieties and terminal sialylated sequences of the secretory products of the mouse SMG of both sexes. We therefore investigated the lectin receptors of control and sialidase-treated sections by a post-embedding approach using horse radish peroxidase (HRP)-conjugates (PNA, DBA, LTA, WGA, Con A), anti-HRP antibody and protein A-gold. Qualitative and quantitative differences occurred in the acinar products of males and females. Sialidase digestion revealed that the acceptor sugar for terminal sialic acids exhibit a different expression in the electron-lucent granules of acinar cells. The occurrence of terminal sialic acid-alpha-N-acetylgalactosamine disaccharide was prominent in male acinar cells in contrast to a modest presence in female. In addition, the terminal sequence sialic acid beta-galactose showed a homogeneous location in the male secretory products and a ...
Atherosclerotic lesions are characterized by lipid infiltration in regions with high rates of endothelial cell turnover. The present investigation was designed to elucidate the route of macromolecular transport across vascular endothelium. The aorta and vena cava of male Sprague-Dawley rats were perfusion-fixed after the intravenous injection of Evans-blue albumin (EBA) or horseradish peroxidase (HRP). Fluorescence microscopic examination of en face preparation of the aorta stained with hematoxylin allowed the identification of endothelial cells that underwent mitosis, together with the localization and quantification of fluorescent spots for EBA leakage. The HRP specimens were subjected to histochemical treatment, and HRP leakage was seen as brown spots under the light microscope. Silver nitrate stain was added in both EBA and HRP studies to outline cell boundaries and to visualize stigmata, stomata, and dead cells. In the aorta, almost every dividing cell showed junctional leakage to albumin ...
article{e3604934-e3c4-4e2c-8fa9-23566d3e0157, abstract = {The role of the electrode material in the efficiency of direct (non-mediated) bioelectrocatalytic reduction of H2O2, catalyzed by horseradish peroxidase (HRP) is studied and discussed. The variations in direct peroxidase bioelectrocatalysis when coming from carbon/graphite to metal electrodes and oxides, as well as modified electrodes, are analyzed regarding the variations in adsorption/orientation of peroxidase at the electrodes, interfacial electron transfer rates and mechanism of catalysis.}, author = {Ferapontova, Elena}, issn = {1040-0397}, keyword = {Modified electrodes,Silver,Gold,Graphite,Horseradish peroxidase,Bioelectrocatalysis}, language = {eng}, number = {13-14}, pages = {1101--1112}, publisher = {John Wiley & Sons}, series = {Electroanalysis}, title = {Direct peroxidase bioelectrocatalysis on a variety of electrode materials}, url = {http://dx.doi.org/10.1002/elan.200403003}, volume = {16}, year = {2004 ...
Author(s): Gurlo, Tatyana; Butler, Peter C; Butler, Alexandra E | Abstract: Immunohistochemistry (IHC) and immunofluorescence (IF) staining techniques are important diagnostic tools of anatomic pathology in the clinical setting and widely used analytical tools in research laboratories. In diabetes research, they are routinely used for the assessment of beta- and alpha-cell mass, for assessment of endocrine cell distribution within the pancreas, for evaluation of islet composition and islet morphology. Here, we present the evaluation of IHC techniques for the detection of alpha-cells in human pancreatic tissue. We compared the Horse Radish Peroxidase (HRP)-based method utilizing DAB Peroxidase Substrate to the Alkaline Phosphatase (AP)-based method utilizing Vector Red substrate. We conclude that HRP-DAB staining is a robust and reliable method for detection of alpha-cells using either rabbit polyclonal or mouse monoclonal anti-glucagon antibodies. However, AP-Vector Red staining should be used with
Avidin and streptavidin reagents are powerful tools to detect or purify biotinylated proteins, nucleic acids, and other macromolecules.
Therapy of experimental leprosy with dried and grated horseradish root administered perorally in a dose of 300 mg/kg mixed food and treatment with purified horseradish peroxidase increased myeloperoxi
Enjoy the flavor of horseradish without the burn! Plentiful Pantry Sea-Horsey Seafood Horseradish Dip Mix is a fabulous dip that combines the mild flavors of horseradish with the rich, but mellow, flavor of seafood. Great for chips and fantastic as a hot dip.
We have developed a novel technique with which to investigate the morphological basis of exocytotic traffic. We have used expression of HRP from cDNA in a variety of cells in combination with peroxidase cytochemistry to outline traffic into and out of the Golgi apparatus at the electron microscopic level with very high sensitivity. A secretory form of the peroxidase (ssHRP) is active from the beginning of the secretory pathway and the activity is efficiently cleared from cells. Investigation of the morphological elements involved in the itinerary of soluble ER proteins using ssHRP tagged with the ER retention motif (ssHRPKDEL) shows that it progresses through the Golgi stack no further than the cis-most element. Traffic between the RER and the Golgi stack as outlined by ssHRPKDEL occurs via vesicular carriers as well as by tubular elements. ssHRP has also been used to investigate the trans side of the Golgi complex, where incubation at reduced temperatures outlines the trans-Golgi network with ...
OPD Tablets for colorimetric detection of Horseradish Peroxidase activity in ELISAs.. Ortho-Phenylenediamine (OPD) is a water soluble chromogenic substrate that produces a yellow color during the enzymatic degradation of hydrogen peroxide by horseradish peroxidase (HRP) with an absorption maximum at 450 nm. After addition of sulfuric acid a very stable orange end solution is obtained that can be read at 490 nm.. Kem-En-Tec Diagnostics offers:. ...
The products we supply are intended for use in accordance with the documentation and manufacturer specifications under the users sole responsibility. Nevertheless, for some specific products (Active Pharmaceutical Ingredients), VWR, as a distributor, is not able to fulfill the requirements (European guidelines for GMP Medicinal products for human and veterinary use) and cannot sell the products.. The products we supply cannot be used as active ingredients, in human or veterinary medicine. For use as pharmaceutical, cosmetic, food additives, agricultural aids, pesticides or domestic, the customer must contact the VWR local sales organisation.. Our customers that intend to use these products for a pharmaceutical, cosmetic, food or any other use must carry out their own internal checks and controls to ensure that they are in compliance with their local laws,regulations (e.g. European pharmacopeia, etc.).. In no event VWR International shall be liable if a customer uses a product that does not fit ...
University of Calgary graduate students retain copyright ownership and moral rights for their thesis. You may use this material in any way that is permitted by the Copyright Act or through licensing that has been assigned to the document. For uses that are not allowable under copyright legislation or licensing, you are required to seek permission. ...
Anti-Human IgM (Fc5u) (Peroxidase Conjugated) Secondary Antibody, Rabbit Polyclonal, Peroxidase (Horseradish) validated in WB, E, IC (ASR2699), Abgent
Horseradish contains pungent compounds, called mustard oils, which are thought to be the constituents which give it the traditionally recognised decongestant effect. Horseradish has been used to help clear the respiratory passages ...
Horseradish is not an instrument either. . of the horseradish plant effers sew kindof health benefit. Threw leaves relieve headaches when Command: are plant fou botany
Want to grow fresh horseradish in your home garden? Get growing tips, read gardening FAQs & learn how to grow horseradish and other vegetables at Burpee. Burpee
Recipe for Holly Cleggs Trim and Terrific Broccoli and Pecans with Creamy Horseradish Sauce. Basic broccoli gets a makeover with toasty pecans and a spunky horseradish sauce.
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Hazarian, Y. H., Khushpulian, D. M., & Tyshkov, V. Y. (2006). Osobennosty struktury y mekhanyzma deistvyia peroksydaz rastenyi. Uspekhy byol. Khymyy, 2(46), 303-322. Hasparov, V. S., & Dehtiarev, V. H. (1994). Opredelenye belka po sviazyvanyiu s krasytelem Kumassy bryllyantovыm holubym G 250. Byokhymyia, 6 (59), 763-777. Hlazova, N. V., &. Serkova, A. N. (2013). Nanostruktury, vkliuchaiushchye peroksydazu, antybyotyky y tsyklodekstryny dlia sozdanyia razlychnykh farmatsevtycheskykh kompozytsyi. Sorbtsyonnye y khromatohrafycheskye protsessy, 3(13), 377-384. Zakharova, H. S., Uporov, Y. V., & Tyshkov, V. Y. (2011). Peroksydaza yz kornei khrena: modulyrovanye svoistv khymycheskoi modyfykatsyei belkovoi hlobuly y hema. Uspekhy byol. khymyy, 2(51), 37-64. Osterman, L. A. (1981). Metody yssledovanyia belkov y nukleynovykh kyslot. Elektroforez y ultratsentryfuhyrovanye. M. Nauka, 288 s. Shannon, L. M., Kay, E., & Lew, I. J. (1979). Peroxidase isosymes from horseradish roots. J. Biol. Chem., 24, ...
The BrDU Cell Proliferation ELISA Kit (ab126556) is an indirect ELISA kit for the detection of BrdU incorporation into newly synthesized DNA of actively proliferating cells. It involves the incorporation of BrdU into cells grown in microtiter plates using the cell layer as the solid phase.. During the last 2 to 24 hours of culture, BrdU is added to the wells of the microtiter plate. BrdU will be incorporated into the DNA of dividing cells. To allow the binding of the antibody to the incorporated BrdU cells, the DNA must be fixed, permeabilized, and denatured.. All of this is done in one step by treating with Fixing Solution. The anti-BrdU detector monoclonal antibody is pipetted into the wells and allowed to incubate for one hour, during which time it binds to any incorporated BrdU. Unbound antibody is washed away and horseradish peroxidase-conjugated goat anti-mouse antibody is added, which binds to the detector antibody.. Horseradish peroxidase catalyzes the conversion of the chromogenic ...
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The rotation in Minnesota is entirely different. The crop again is in for two years, then harvested. After that, a neighbor plants wild rice on those acres for two years. After each rice harvest, the fields are flooded with water for 60 days. This is based on an old University of Minnesota recommendation for soilborne disease control, and flooding is facilitated by the bordered fields on that farm. The next year, soybeans are planted for one year, with another horseradish crop to follow that.. Traaseth acknowledges that he hasnt carried out any large-scale experiments to verify that either of these methods is the best way to achieve disease control, but he says that as a result of this program, the disease complex does not persist and their fields are clean. In fact, as long as this rotation is in effect, he does not consider that the farm has a disease problem, even though they use their own lateral roots for sets just as everybody else does. In fact, their clean sets are in demand from other ...
Horseradish peroxidase is used in biochemistry applications such as western blots, ELISA and Immunohistochemistry. It is used to amplify a weak signal and increase detectability of a target molecule, such as a protein. HRP labeled immunoglobulins are used as probes for the demonstration of tissue antigens and in enzyme immunoassay (EIA) systems for determination of soluble and insoluble antigens. HRP is the most desired label for antibodies since it is the smallest and most stable of the three most popular enzyme labels (HRP, alkaline phosphatase, and B-galactosidase) and its glycosylation leads to lower non-specific binding. It is also useful for tracing neural connections. These include motor and sensory innervation of peripheral organs, and connections of peripheral nerves, ganglia and individual dorsal roots. HRP can be conjugated to antibodies by several different methods including glutaraldehyde, periodate oxidation, through disulfide bonds, and also via amino and thiol directed ...
Philopena, Jennifer wrote: > Hi All. > I have an HRP-conjugated primary antibody that works very well for IHC (and > Id like to keep using it), but I want a fluorescent signal. Does anyone > know of a fluor-conjugated anti-HRP antibody? A solution would be to use a fluorochrome-conjugated tyramide reagent. The peroxidase converts the tyramide into a radical that rapidly forms covalent bonds with adjacent proteins, meaning that in effect you get lots of fluorochrome bound around the antigen (dont get me wrong, the spatial resolution is still OK). This is easy (after HRP-antibody you just add the tyramide reagent, incubate 10 min or so, wash, and thats it) and should produce pretty strong signal (I have myself used only biotinylated tyramide, but the principle is the same) . The only drawback I can think of is that the stuff is pretty expensive. Its patented by NEN and sold also by a few other companies (I think DAKO and perhaps Molecular Probes). -- ...
Luminol Reagent is recommended for Western blotting enhanced chemiluminescence applications using Horseradish Peroxidase (HRP) conjugated secondary antibodies.
Secondary antibodies that are designed for use in Western blots and ELISA are generally conjugated to enzymes, such horseradish peroxidase or alkaline phosphatase. Biotin-conjugated secondary antibodies are also commonly used.
Secondary antibodies that are designed for use in Western blots and ELISA are generally conjugated to enzymes, such horseradish peroxidase or alkaline phosphatase. Biotin-conjugated secondary antibodies are also commonly used.
Hyperimmune goat IgG against horseradish peroxidase reacted with peroxidase lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2). 0.005% Merthiolate ...
Azithromycin reduces about 40% of IL-8 mRNA and protein expression in cystic fibrosis (CF) cells reaching the levels of non-CF cells. Azithromycin results in 50% and 70% reduction of NF-kappaB and AP-1 DNA binding, respectively, leading to levels of non-CF cells. [1] Azithromycin significantly enhances the intensity of a co-stimulatory molecule, CD80, on DCs but not CD86 and CD40 in dendritic cells (DCs). Azithromycin significantly increases the production of IL-10 and Clarithromycin (CAM) significantly inhibits the production of IL-6 by DCs. Azithromycin increases IL-10 and CAM decreases IL-2 productions significantly, when naive T cells derived from spleen are co-cultured with DCs treated in advance with LPS and these macrolides. [2] Azithromycin selectively inhibits fluid-phase endocytosis of horseradish peroxidase and lucifer yellow in J774 mouse macrophages. Azithromycin delays sequestration of receptor-bound transferrin and peroxidase-anti-peroxidase immune complexes into cell-surface ...
A small diameter flexible electrode designed for subcutaneous in vivo amperometric monitoring of glucose is described. The electrode is designed to allow one-point in vivo calibration, i.e., to have zero output current at zero glucose concentration, even in the presence of other electroreactive species of serum or blood. The electrode is preferably three or four-layered, with the layers serially deposited within a recess upon the tip of a polyamide insulated gold wire. A first glucose concentration-to-current transducing layer is overcoated with an electrically insulating and glucose flux limiting layer (second layer) on which, optionally, an immobilized interference-eliminating horseradish peroxidase based film is deposited (third layer). An outer (fourth) layer is biocompatible.
Glucose oxidase (GOx) and horseradish peroxidase (HRP) are important enzymes for the development of amperometric enzyme linked immunosensors. The selectivity of each enzyme towards its analyte deepens its importance in ...
Primary antibodies are versatile biological reagents used to detect a wide range of antigenic targets. Antibodies against a target of interest are raised in host species such as mouse, sheep, rabbit, rat or goat. The target is often a protein, and is usually from a species different to that of the host. Common target species are human, mouse, rat and veterinary species, but can also be viral, bacterial or chemical depending on the area of research. Our catalogue of high quality monoclonal and polyclonal primary antibodies are suitable for use in numerous applications, including flow cytometry, ELISA, immunohistochemistry, immunofluorescence and western blot. They are available labelled with a variety of fluorescent dyes or proteins including FITC, PE, PerCP and APC, enzymes such as horseradish peroxidase, or biotin for further amplification via labelled streptavidin. Also available without a label, our unconjugated antibodies require a species-specific secondary antibody to enable detection. ...
VisiBlot Standard I is a mixture of 10 recombinant proteins of a molecular weight range from 25 kDa to 150 kDa. Protein bands of 25 kDa, 45 kDa and 85 kDa are prestained allowing monitoring of protein separation during SDS PAGE. The remaining five proteins contain several IgG binding sites. Hence marker proteins bind to primary or secondary antibodies used in Western Blotting facilitating easy marker visualization on the transfer membrane. Because the proteins have no chromophore attached, the marker enables accurate molecular weight estimation. Recommended loading volume for a mini gel is 5 µl/lane. Ready-to-use, no reconstitution, further dilution or heating required Prestained bands for monitoring electrophoresis and membrane transfer Visualization of marker proteins on Western Blots by horseradish peroxidase or alkaline phosphatase-based immune-detection methods Molecular weight determination of proteins detected on transfer membrane
Sun-Sang J. Sung; Rolf S. Nelson; Samuel C. Silverstein (jul 1983). „The role of the mannose/N-acetylglucosamine receptor in the pinocytosis of horseradish peroxidase by mouse peritoneal macrophages. Journal of Cellular Physiology. 116: 21-25. doi:10.1002/jcp.1041160105 ...
Affinity purified rabbit polyclonal anti-acetylated lysine antibody (anti-AcK) was developed using a unique technique that utilizes acetylated KLH as the immunogen. The purified antibody was conjugated to horseradish peroxidase (HRP) via reductive amination. Direct labelling of the primary antibody will avoid the use of secondary antibodies therefore eliminating the interference of the secondary antibody-conjugates ...
Rabbit Anti Mouse Vitronectin Polyclonal Fractionated HRP Labeled from Innovative Research is a polyclonal antibody in a Frozen liquid format. This antibody has been purified using protein A affinity chromatography followed by horseradish peroxidase conjugation. Fractionated preparations may be especially useful in res
TMB can act as a hydrogen donor for the reduction of hydrogen peroxide to water by peroxidase enzymes such as horseradish ... "The light side of horseradish peroxidase histochemistry". J Histochem Cytochem. 32 (7): 793. doi:10.1177/32.7.6736628. PMID ... peroxidase. The resulting diimine causes the solution to take on a blue colour, and this colour change can be read on a ...
A study with horseradish peroxidase in the monkey". J. Hirnforsch. 23: 257-269. Zhang, S., Ide, J.S., and Li, C.S. (2012). " ...
The effect of peroxidases in horseradish on guiacum was first noted in 1810. As a food additive, Guaiacum is designated E314 ... 2003). "Horseradish peroxidase: a valuable tool in biotechnology". Biotechnol Annu Rev. Biotechnology Annual Review. 9: 199-247 ...
Armarego WL, Ohnishi A, Taguchi H (1986). "New pteridine substrates for dihydropteridine reductase and horseradish peroxidase ...
In 1978, horseradish peroxidase (HRP) was introduced as a marker. HRP was more effective than previous markers, but required ...
"Oculomotor nucleus afferents in the monkey demonstrated with horseradish peroxidase". Brain Research. 160 (1): 1-15. doi: ...
"DNA-directed immobilization of horseradish peroxidase onto porous SiO2 optical transducers". Nanoscale Research Letters. 7 (1 ...
The interaction of soluble horseradish peroxidase in mouse peritoneal macrophages in vitro. J. Cell Biol. 55:186-204. With R. ...
An intracellular horseradish peroxidase study". Brain Research. 183 (2): 253-263. doi:10.1016/0006-8993(80)90462-X. ISSN 0006- ...
... can also be polymerized using the horseradish peroxidase/H2O2 oxidizing system. Caffeic acid may be the active ... "Peroxidase-Catalyzed in Situ Polymerization of Surface Orientated Caffeic Acid". J. Am. Chem. Soc. 127 (33): 11745-11753. doi: ...
The Horseradish Peroxidase/ o-Phenylenediamine (HRP/OPD) System Exhibits a Two-Step Mechanism. M. K. Tiama and T. M. Hamilton, ...
"Tracing axons and axon collaterals of spinal neurons using intracellular injection of horseradish peroxidase". Science. 191 ( ...
Enzymes used in ELISAs include horseradish peroxidase (HRP), alkaline phosphatase (AP) or glucose oxidase. These enzymes allow ...
... a horseradish peroxidase study". J. Anat. 210 (4): 428-38. doi:10.1111/j.1469-7580.2007.00709.x. PMC 2100289. PMID 17428204. ...
Like horseradish peroxidase, lanthanum is used as an electron-dense tracer in molecular biology. Lanthanum-modified bentonite ( ... of the blood-ganglion barrier properties in rat sympathetic ganglia by using lanthanum ion and horseradish peroxidase as ...
Kressel M (April 1998). "Tyramide amplification allows anterograde tracing by horseradish peroxidase-conjugated lectins in ... exocytic pathways of the neuronal secretory process and trans-synaptic transfer of wheat germ agglutinin-horseradish peroxidase ...
A secondary anti-human antibody conjugated to an enzyme such as horseradish peroxidase is added. The enzyme reaction will ...
Demonstration of bilateral projection of the central retina of the monkey with horseradish peroxidase neuronography. Journal of ...
Jeong YM, Oh MH, Kim SY, Li H, Yun HY, Baek KJ, Kwon NS, Kim WY, Kim DS (2010). "Indole-3-acetic acid/horseradish peroxidase ... In 2002 it had been hypothesized that IAA coupled with horseradish peroxidase (HRP) could be used in targeted cancer therapy. ... Wardman P (2002). "Indole-3-acetic acids and horseradish peroxidase: a new prodrug/enzyme combination for targeted cancer ... "Antibody-targeted horseradish peroxidase associated with indole-3-acetic acid induces apoptosis in vitro in hematological ...
A one-electron oxidation of carcinogenic nonaminoazo dye Sudan I by horseradish peroxidase. Neuro Endocrinology Letters 2008, ... It is suggested that a CYP- or peroxidase-mediated activation of Sudan I or a combination of both mechanisms as an explanation ... In addition to oxidation by CYP enzymes, Sudan I and its C-hydroxylated metabolites are also oxidized by peroxidases, such as a ... This supports the explanation that Sudan 1 is oxidized or activated by peroxidase in the blood cells and thereby forming micro- ...
"Enhanced stability of horseradish peroxidase encapsulated in acetalated dextran microparticles stored outside cold chain ...
Vogel Z, Towbin M, Daniels MP (April 1979). "Alpha-bungarotoxin-horseradish peroxidase conjugate: preparation, properties and ...
Secondary antibodies can be conjugated to enzymes such as horseradish peroxidase (HRP) or alkaline phosphatase (AP); or ...
After further washing, the color is developed with streptavidin labelled horse radish peroxidase with TMP as substrate. A ... AZTMP is measured in an immunoassay with anti-AZTMP antibodies and AZTMP-labeled peroxidase. The assay runs in a closed system ...
... a combined retrograde and anterograde horseradish peroxidase study in the rat". Brain Res. 513 (1): 43-59. doi:10.1016/0006- ...
Martell, JD (2016). "A split horseradish peroxidase for the detection of intercellular protein-protein interactions and ... split horseradish peroxidase for visualization of synapses in vivo, FLARE for gaining genetic access to activated neural ... Paek, J (2017). "Multidimensional Tracking of GPCR Signaling via Peroxidase-Catalyzed Proximity Labeling". Cell. 169: 338-349. ... Martell, JD (2012). "Engineered ascorbate peroxidase as a genetically encoded reporter for electron microscopy". Nature ...
"The structure of a neural specific carbohydrate epitope of horseradish peroxidase recognized by antihorseradish peroxidase ... 1991, Japanese researchers determined the structure of the epitope common to horseradish peroxidase and Drosophila neurons as ...
... epsilon-viniferin by horseradish peroxidase. Phenolic content in wine Viniferin (disambiguous) alpha-Viniferin epsilon- ... "Structures of Two Novel Trimeric Stilbenes Obtained by Horseradish Peroxidase Catalyzed Biotransformation oftrans-Resveratrol ...
"Covalent attachment of cholesterol oxidase and horseradish peroxidase on perlite through silanization: Activity, stability and ...
Second, the horseradish peroxidase utilizes the hydrogen peroxide to oxidize ABTS to its radical cationic form, ABTS+•. As the ... The experiment is performed in a clear glass vial containing two common enzymes (glucose oxidase and horseradish peroxidase), ...
Peroxidase. References[edit]. *^ a b PDB: 1VAR​; Borgstahl GE, Parge HE, Hickey MJ, Johnson MJ, Boissinot M, Hallewell RA, ... SOD is commercially obtained from marine phytoplankton, bovine liver, horseradish, cantaloupe, and certain bacteria. For ...
HpODE is rapidly reduced by peroxidases to 13(S)-HODE.[1][5] ALOX15 is fully capable of metabolizing the linoleic acid that is ... Allyl isothiocyanate (mustard, radish, horseradish, wasabi). *AM404. *Bradykinin. *Cannabichromene (cannabis). *Cannabidiol ( ...
TAT was able to deliver different proteins, such as horseradish peroxidase and RNase A across cell membrane into the cytoplasm ...
Horseradish peroxidase. *Lactoperoxidase. *Myeloperoxidase. *Thyroid peroxidase. *Deiodinase *Iodothyronine deiodinase. * ... Peroxidases,state=autocollapse}} *shows the template collapsed to the title bar if there is a {{navbar}}, a {{sidebar}}, or ... Peroxidases,state=collapsed}} to show the template collapsed, i.e., hidden apart from its title bar ... Peroxidases,state=expanded}} to show the template expanded, i.e., fully visible ...
... a combined retrograde and anterograde horseradish peroxidase study in the rat". Brain Research. 513 (1): 43-59. doi:10.1016/ ...
Using horseradish peroxidase to stain the neurons, it has been determined that the external anal sphincter motoneurons are ...
Injecting wheat germ agglutinin and horseradish peroxidase conjugate into the anterior cingulate gyrus of cats, revealed that ...
... (GPx) (EC 1.11.1.9) is the general name of an enzyme family with peroxidase activity whose main ... Horseradish peroxidase. *Lactoperoxidase. *Myeloperoxidase. *Thyroid peroxidase. *Deiodinase *Iodothyronine deiodinase. * ... glutathione peroxidase 4 (phospholipid hydroperoxidase) GPX5. Chr. 6 p21.32. glutathione peroxidase 5 (epididymal androgen- ... Glutathione peroxidase 2 is an intestinal and extracellular enzyme, while glutathione peroxidase 3 is extracellular, especially ...
The role of the mannose/N-acetylglucosamine receptor in the pinocytosis of horseradish peroxidase by mouse peritoneal ...
... the presence of catalase or other peroxidases in these organisms can increase tolerance in the presence of lower concentrations ... Allyl isothiocyanate (mustard, radish, horseradish, wasabi). *AM404. *Bradykinin. *Cannabichromene (cannabis). *Cannabidiol ( ...
Ensüümina kasutatakse HRP (horseradish peroxidase) või AP (alkaline phosphatase), mis on kovalentselt seotud antikehaga[1]. ... Horseradish peroxidase on peamine reporterensüüm kemoluminestsentsetes substraatides. HRP on väike (40 kDa), stabiilne ja odav ... Horseradish peroxidase´ga seotud sekundaarset antikeha kasutatakse kemoluminestsentse agendi lõhestamiseks. Sellega tagatakse ...
Horseradish peroxidase[8]. *Infrared fluorescent protein IFP1.4, an engineered chromophore-binding domain (CBD) of a ... "A split horseradish peroxidase for the detection of intercellular protein-protein interactions and sensitive visualization of ...
Horseradish peroxidase. *Lactoperoxidase. *Myeloperoxidase. *Thyroid peroxidase. *Deiodinase *Iodothyronine deiodinase. * ... "Entrez Gene: glutathione peroxidase 6 (olfactory)".. *^ Kryukov GV, Castellano S, Novoselov SV, Lobanov AV, Zehtab O, Guigó R, ... Glutathione peroxidase 6 (GPx-6) is an enzyme that in humans is encoded by the GPX6 gene.[5][6] ... This gene product belongs to the glutathione peroxidase family, which functions in the detoxification of hydrogen peroxide. It ...
While the list of enzyme substrates is extensive, alkaline phosphatase (AP) and horseradish peroxidase (HRP) are the two ... In the most common instance, an antibody is conjugated to an enzyme, such as peroxidase, that can catalyse a colour-producing ...
... of the blood-ganglion barrier properties in rat sympathetic ganglia by using lanthanum ion and horseradish peroxidase as ...
It is commonly used as a substrate with hydrogen peroxide for a peroxidase enzyme (such as horseradish peroxidase) or alone ... Its use allows the reaction kinetics of peroxidases themselves to be followed. In this way it also can be used to indirectly ...
... immobilized horseradish peroxidase (HRP) converts tyramide into reactive intermediate in the presence of hydrogen peroxide. ... For signal amplification, slides are incubated with streptavidin-biotin-peroxidase complex followed by biotinyl-tyramide/ ... hydrogen peroxide and streptavidin-peroxidase. Development is completed using hydrogen peroxide and scans of the slides are ...
Horseradish peroxidase, an enzyme used as a marker HRP Rescuer, an American helicopter Humanoid Robotics Project Human Research ...
... and horseradish peroxidase. Phillips, Roger (2010). Mushrooms and Other Fungi of North America. Buffalo, NY: Firefly Books. p. ...
"A comparison of the intranigral distribution of nigrotectal neurons labeled with horseradish peroxidase in the monkey, cat and ...
... and analytical applications and are based upon the oxidation of luminol in the presence of the enzyme horseradish peroxidase ( ...
Similarly, for the detection of IgG, IgG can be immobilized onto a DMF chip, conjugated with horseradish-peroxidase (HRP)- ...
... wheat germ agglutinin-horseradish peroxidase conjugate See List of MeSH codes (D12.776.543). MeSH D12.776.556.579.249.290 - ... wheat germ agglutinin-horseradish peroxidase conjugate MeSH D12.776.826.701.500.500 - Retinoid X receptor alpha MeSH D12.776. ...
Horseradish Peroxidase, Plastocyanin, and Laccase. Penner-Hahn's research involves biophysical chemistry and inorganic ...
The enzyme horseradish peroxidase (HRP), found in the roots of horseradish, is used extensively in biochemistry applications. ... Horseradish peroxidase is ideal in many respects for these applications because it is smaller, more stable, and less expensive ... Horseradish peroxidase is also commonly used in techniques such as ELISA and Immunohistochemistry due to its monomeric nature ... Numerous substrates for horseradish peroxidase have been described and commercialized to exploit the desirable features of HRP ...
Definition of horseradish peroxidase. Provided by Stedmans medical dictionary and Drugs.com. Includes medical terms and ... horseradish peroxidase. Definition: a peroxidases isolated from horseradish that is used in immunohistochemistry to label the ...
... BMC Genomics. 2014 Mar 24;15:227. doi: 10.1186/1471-2164-15-227. ... Background: Horseradish peroxidases (HRPs) from Armoracia rusticana have long been utilized as reporters in various diagnostic ... Identification and manual verification of the sequences of 28 HRP isoenzymes do not only contribute a set of peroxidases for ... Sequence databases, ORF finding and ORF characterization were utilized to identify peroxidase genes from the 14871 isotigs ...
The radiation-induced inactivation of horseradish peroxidase in dilute aqueous solutions has been studied. It has been found ... The radiation-induced inactivation of horseradish peroxidase in dilute aqueous solutions has been studied. It has been found ... The radiation-induced inactivation and post irradiation effects of horseradish peroxidase solution. ...
Horseradish Peroxidase Market - Scope of the Report TMRs report on the global horseradish peroxidase market studies past as ... Horseradish Peroxidase Market: Global Industry Analysis, Size, Share, Growth, Trends, and Forecast, 2020 - 2030. ... Horseradish Peroxidase Market - Scope of the Report. TMRs report on the global horseradish peroxidase market studies past as ... Horseradish Peroxidase Market: Global Industry Analysis, Size, Share, Growth, Trends, and Forecast, 2020 - 2030. Aug 2020, ...
Development of horseradish peroxidase/layered double hydroxide hybrid catalysis for phenol degradation. ... C was used for horseradish peroxidase (HRP) immobilization through absorption. The structural, optical and thermal properties ...
SouthernBiotech monoclonal secondary antibodies offer high sensitivity and low background for use in a variety of applications. Shop IgG1 Rat anti-Mouse, HRP, Clone: SB77e,
The reaction between the TMB substrate and a peroxidase, typically horseradish peroxidase (HRP), produces a measureable color ... Horseradish peroxidase was used to label the regenerative axons of peroneal nerves 4 or 9 weeks after epineurial or Perineurial ... A voltammetric enzyme - linked immunoassay based on a new system of o- tilidine (OT) - H2O2 - horseradish peroxidase (HRP) has ... Based on the inhibition of cyanide to the horseradish peroxidase(HRP), the HRP electrode has been used in the determination of ...
Abcepta provides over 30,000 primary antibodies of the highest quality for your research. Our scientists now manufacture over 4,000 antibodies per year!
Horseradish Peroxidase. 4/2009. Tumor apoptosis by indole-3-acetic acid/light in B16F10 melanoma-implanted nude mice.. ... Indole-3-acetic acid/horseradish peroxidase-induced apoptosis involves cell surface CD95 (Fas/APO-1) expression.. ... Hydrogen peroxide is a mediator of indole-3-acetic acid/horseradish peroxidase-induced apoptosis.. ...
Monoclonal Anti-GABA A Receptor-Horseradish Peroxidase antibody produced in mouse clone S413-67; find Sigma-Aldrich-SAB5202233 ... Monoclonal Anti-GABA A Receptor-Horseradish Peroxidase antibody produced in mouse clone S413-67 * NACRES NA.45 ... SAB5202233 - Monoclonal Anti-GABA A Receptor-Horseradish Peroxidase antibody produced in mouse ...
Horseradish peroxidase (HRP) 500 Test, Rabbit, Detection Kit (SIGNET) from Covance Research Products, Inc,Each Signet Level 2 ... Horseradish Peroxidase Conjugated from Abcam. 7. Peroxidase, Horseradish, Conjugate-Stabilizing Solution from Calbiochem. 8. ... Horseradish Peroxidase Conjugated from Meridian Life Science, Inc.. 9. Horseradish Peroxidase (HRP) Conjugation Kit For ... Ultrastreptavidin (USA) Horseradish peroxidase (HRP) 50 Test, Multi-Species, AEC Detection Kit (SIGNET) from Covance Research ...
Peroxidase from horseradish has been used in western blotting. Bulk and Prepack available at Sigmaaldrich.com. ... The isoelectric point for horseradish peroxidase isozymes ranges from 3.0 - 9.0. Application Peroxidase from horseradish has ... Horseradish peroxidase is isolated from horseradish roots (Amoracia rusticana) and belongs to the ferroprotoporphyrin group of ... Peroxidase from horseradish Suitable for manufacturing of diagnostic kits and reagents Synonym: Donor:hydrogen-. peroxide ...
ADHP is a non-fluorescent compound that reacts with horseradish peroxidase (HRP) in the presence of peroxide to produce ... or horse radish peroxidase (HRP), respectively, on western blots or dot blots. The sensitivity of the AP substrate is in the ... which forms a blue precipitate upon reaction with horse radish peroxidase. It is available as a standalone product (Cat. No. ...
Horseradish Peroxidase)]. Available in 15 dyes & fluorophores. Backed by our 100% Guarantee. ... Be the first to review our Donkey anti-Goat IgG Secondary Antibody [HRP (Horseradish Peroxidase)] and receive a gift card or ... Donkey anti-Goat IgG Secondary Antibody [HRP (Horseradish Peroxidase)] Summary. Immunogen. Goat IgG ... Alternate Names for Donkey anti-Goat IgG Secondary Antibody [HRP (Horseradish Peroxidase)]. *IgG ...
A Chemical Synthesis of Benzo[a]pyrene Hapten and Its Application in a Streptavidin-Horseradish Peroxidase-Based Enzyme-Linked ... Based on these works, a streptavidin-horseradish peroxidase-based enzyme-linked immunological analysis was developed and ...
Global Horseradish Peroxidase (HRP) Market Data Survey Report 2025 is a market research report available at US $1500 for a ... Global Horseradish Peroxidase (HRP) Market Research Report 2018. Global Horseradish Peroxidase (HRP) Sales Market Report 2018. ... Pierce Horseradish Peroxidase (HRP) is purified horseradish peroxidase enzyme for use in activity assays and conjugation to ... Figures Global Horseradish Peroxidase (HRP) Market Size and CAGR 2011-2017 (Value). Figures Global Horseradish Peroxidase (HRP ...
Expression of recombinant horseradish peroxidase C in Escherichia coli. JULIAN F. BURKE, ANDREW SMITH, NIOVI SANTAMA, ROBERT C. ... Expression of recombinant horseradish peroxidase C in Escherichia coli. JULIAN F. BURKE, ANDREW SMITH, NIOVI SANTAMA, ROBERT C. ... Expression of recombinant horseradish peroxidase C in Escherichia coli Message Subject (Your Name) has forwarded a page to you ...
What is horseradish peroxidase? Meaning of horseradish peroxidase medical term. What does horseradish peroxidase mean? ... Looking for online definition of horseradish peroxidase in the Medical Dictionary? horseradish peroxidase explanation free. ... Related to horseradish peroxidase: alkaline phosphatase. horse·rad·ish per·ox·i·dase. a peroxidases isolated from horseradish ... 2) and a mixture of horseradish peroxidase isoenzymes (pI 6.. Accumulation of a Basic Peroxidase Isoenzyme in Leaves of ...
horseradish peroxidase; soybean peroxidase review; biosensors; biocatalysis; hutagenesis. Subjects:. Biological Sciences , ... Ryan, Barry J., Carolan, Neil and Ó Fágáin, Ciarán (2006) Horseradish and soybean peroxidases: comparable tools for alternative ... Horseradish and soybean peroxidases (HRP and SBP, respectively) are useful biotechnological tools. HRP is often termed the ... The final paragraphs speculate on the future of plant heme-based peroxidases, with probable trends outlined and explored. ...
... is produced by our own coupling procedure which preserves the high specific activity of the peroxidase. ... Horseradish Peroxidase Avidin D is offered as a concentrate (5 mg/ml) or in a ready-to-use (R.T.U.) stabilized solution (100ml, ... ImmPACT® AEC Substrate, Peroxidase (HRP) Catalog #: SK-4205 AEC Substrate Kit, Peroxidase (HRP), (3-amino-9-ethylcarbazole) ... For horseradish peroxidase labeled Avidin D (Av-HRP) that is optimized for use in solid-phase assays such as ELISAs and ...
button size=small text=MSDS & Datasheet link=/wp-content/uploads/media/BCDatasheets_C_10.26/BAK/BAK-004.pdf]Horseradish ... Horseradish Peroxidase Avidin Kit. Kit Contents: 5mg of the described Avidin Conjugate and 1mg each of the following Biotin ... SKU: BAK-004 Category: Glycoprotein Analysis Kits Tags: kits, horseradish-peroxidase, Avidin, BAK-004 ...
Horseradish Peroxidase Alcohol Oxidase Poly (vinyl alcohol) azide-unit water pendant (PVA-AWP) immobilization Biosensor. ... Evaluation of immobilizing horseradish peroxidase and alcohol oxidase in PVA-AWP polymer. Welcome to the IDEALS Repository. ... Evaluation of immobilizing horseradish peroxidase and alcohol oxidase in PVA-AWP polymer. Beesabathuni, Shilpa N. ... Evaluation of immobilizing horseradish peroxidase and alcohol oxidase in PVA-AWP polymer. ...
SKU: BA-110-1 Categories: Avidin or Streptavidin Biotin, Horseradish Peroxidase Tags: horseradish-peroxidase, Strept.-Avidin, ... Home / Avidin or Streptavidin Biotin / Horseradish Peroxidase. Strept. Avidin Horseradish Peroxidase, 1mg. ...
Horseradish Peroxidase Service 91-1123 Enzyme/Protein Conjugation: Horseradish Peroxidase Service 91-1123 ... Home Page >Online Product Catalog > Custom DNA & Gene Synthesis > Enzyme/Protein Conjugation: Horseradish Peroxidase (Service) ...
Peroxidase Conjugated) IgM (Mu Chain) Secondary Antibody, Goat Polyclonal, Peroxidase (Horseradish) validated in (ASR1011), ... Anti-Mouse (Peroxidase Conjugated) IgM (Mu Chain) Secondary Antibody. Goat Polyclonal, Peroxidase (Horseradish). ... home , Products , Secondary Antibodies , Peroxidase (Horseradish) , Anti-Mouse (Peroxidase Conjugated) IgM (Mu Chain) Secondary ... Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum, Mouse IgM and ...
Horse Radish Peroxidase (HRP) Enzyme, purified enzyme EW-HRP15-N ... Horse Radish Peroxidase (HRP) Enzyme, purified enzyme EW-HRP15- ... Horse Radish Peroxidase (HRP) Enzyme, purified (enzyme) A-Z Product Search ...
Global Horseradish Peroxidase (HRP) Market 2021 by Manufacturers, Regions, Type and Application, Forecast to 2026 is a recent ... Global Horseradish Peroxidase (HRP) Market Analysis Report with Investment Feasibility and Trends 2026. Global Horseradish ... Global Horseradish Peroxidase (HRP) Market 2021 by Manufacturers, Regions, Type and Application, Forecast to 2026 is a recent ... Home/Business/Global Horseradish Peroxidase (HRP) Market Analysis Report with Investment Feasibility and Trends 2026. ...
... employing the enzymes glucose oxidase and horseradish peroxidase, with direct electron transfer of horseradish peroxidase at a ... A theoretical model consistent with this outcome suggests that the kinetic rates of horseradish peroxidase were greatly reduced ... employing the enzymes glucose oxidase and horseradish peroxidase, with direct electron transfer of horseradish peroxidase at a ... Optimising the ratio of horseradish peroxidase and glucose oxidase on a bienzyme electrode: comparison of a theoretical and ...
Horseradish Peroxidase Avidin D is offered as a concentrate (5 mg/ml) or in a ready-to-use (R.T.U.) stabilized solution (100ml, ... For horseradish peroxidase labeled Avidin D (Av-HRP) that is optimized for use in solid-phase assays such as ELISAs and ... Horseradish Peroxidase Avidin D (HRP Avidin D) is produced by our own coupling procedure which preserves the high specific ... Home Enzyme Conjugated Avidin/Streptavidin Horseradish Peroxidase Avidin D (Av-HRP), Ready-to-Use (R.T.U.) ...
  • In addition to biomedical applications, horseradish peroxidase is one of the enzymes with important environmental applications. (wikipedia.org)
  • 2002) entrapped horseradish peroxidase enzymes within silica sol-gel matrices and re-used this catalytic material for multiple reaction cycles. (thefreedictionary.com)
  • The biosensor is based on a bi-enzyme channelling configuration, employing the enzymes glucose oxidase and horseradish peroxidase, with direct electron transfer of horseradish peroxidase at a conducting polymer electrode. (dit.ie)
  • Oxidative enzymes such as lignin peroxidase, horseradish peroxidase (HRP), manganese peroxidase and phenoloxidase (laccase) are extensively employed to remove color from effluent by oxidative degradation of colored compounds and to degrade toxic polyphenols, polyaromatic hydrocarbons, polychlorinated biphenyls, etc. (springeropen.com)
  • One of the most widely used enzymes for chromogenic detection in immunoassays is horseradish peroxidase (HRP), a highly stable enzyme (Clin. (bmrservice.com)
  • For immunostaining of mammalian cells, an advantage of using anti-horseradish peroxidase is reduced background, since the antibody does not recognize the endogenous peroxidase-like enzymes found in those cells. (jacksonimmuno.com)
  • These biosensors are complexes of graphene oxide and biomacromolecules, including enzymes such as glucose oxidase, horseradish peroxidase, laccase, and nucleic acids such as DNA and RNA. (intechopen.com)
  • Horseradish peroxidase represents one of the most exploited enzymes in the process of enzymatic phenol removal from aqueous solutions. (stumejournals.com)
  • TMB can act as a hydrogen donor for the reduction of hydrogen peroxide to water by peroxidase enzymes such as horseradish peroxidase. (wikipedia.org)
  • Secondary Antibodies-Horse Radish Peroxidase-Biotin Conjugate (Biotin-HRP)-Alpha Diagnostic International Inc. (4adi.com)
  • Antibodies specific for horseradish peroxidase (HRPeroxase) bind to neuronal membranes in Drosophila and serve as a specific neuronal marker. (pnas.org)
  • Pierce Horseradish Peroxidase (HRP) is purified horseradish peroxidase enzyme for use in activity assays and conjugation to antibodies for ELISA, Western blot and Research applications. (rnrmarketresearch.com)
  • Antibodies in the Chromocyte database for 14-3-3 Tau and Horseradish Peroxidase (HRP). (chromocyte.com)
  • Antibodies in the Chromocyte database for Abhydrolase domain containing 6 / ABHD6 and Horseradish Peroxidase (HRP). (chromocyte.com)
  • Ready-to-use substrate solution for chemiluminescence detection of membrane bound antigens or nucleic acid sequences directly with Horseradish Peroxidase (HRP) or indirectly with HRP-conjugated antibodies or Streptavidin labeled. (biophoretics.com)
  • The Expedeon Lightning-Link® Horseradish Peroxidase (HRP) antibody labeling kit enables the direct conjugation of antibodies, proteins and peptides, or any other biomolecule with an available amine group, to HRP with only 30 seconds hands-on time and with 100% recovery of materials. (expedeon.com)
  • Antibodies conjugated with horseradish peroxidase are one of the most widely used bioreagents in the biological sciences. (antibodiesmanual.org)
  • Invitrogen AP Chromogenic Substrate (BCIP/NBT) and Invitrogen HRP Chromogenic Substrate (TMB) are ready-to-use, single component substrates for sensitive immunodetection of alkaline phosphatase (AP) or horse radish peroxidase (HRP), respectively, on western blots or dot blots. (thermofisher.com)
  • Invitrogen HRP Chromogenic Substrate consists of a ready-to-use non-toxic solution of 3,3',5,5'-tetramethylbenzidine (TMB) which forms a blue precipitate upon reaction with horse radish peroxidase. (thermofisher.com)
  • enzyme-Horse Radish Peroxidase (HRP) Enzyme, purified-Genemed Synthesis Inc. (genemedsyn.com)
  • A novel method of immobilizing horse radish peroxidase enzyme (HRP) onto the surface of gold nanoparticles (GNPs) was developed. (edu.pl)
  • The heme vicinities of the acid and alkaline forms of native (Fe(III)) horse- radish peroxidase were investigated in terms of the magnetic circular dichroism (MCD) spectroscopy. (elsevier.com)
  • Tropolone inhibits both the monophenolase and dihydroxyphenolase activity of mushroom tyrosinase (Kahn and Adrawis 1985a), but in the presence of hydrogen peroxide, it can serve as a substrate for horseradish peroxidase (Kahn & Andrawis 1985b). (thefreedictionary.com)
  • 1% Tween 20/PBS, and incubated with 4-chloro-1-naphthol solution (substrate of horseradish peroxidase in the kit) containing 0. (thefreedictionary.com)
  • Further, these nanoporous TiO 2 electrodes are employed as a substrate for enzyme ( horseradish peroxidase , HRP) immobilization in an attempt to enhance the electron transport across the semiconductor electrode-electrolyte interface. (springer.com)
  • Here, we show the degradation of C. I. Acid blue 113 using horseradish peroxidase (HRP) assisted with H 2 O 2 as a co-substrate. (springeropen.com)
  • TMR's report on the global horseradish peroxidase market studies past as well as current growth trends and opportunities to gain valuable insights of these indicators of the market during the forecast period from 2020 to 2030. (bccresearch.com)
  • The report provides revenue of the global horseradish peroxidase market for the period 2018-2030, considering 2019 as the base year and 2030 as the forecast year. (bccresearch.com)
  • The report also provides the compound annual growth rate (CAGR) for the global horseradish peroxidase market during the forecast period. (bccresearch.com)
  • Extensive secondary research involved referring to key players' product literature, annual reports, press releases, and relevant documents to understand the global horseradish peroxidase market. (bccresearch.com)
  • Analysts have employed a combination of top-down and bottom-up approaches to study various phenomenon in the global horseradish peroxidase market. (bccresearch.com)
  • Furthermore, the report sheds light on the changing competitive dynamics in the global horseradish peroxidase market. (bccresearch.com)
  • These indices serve as valuable tools for existing market players as well as for entities interested in entering the global horseradish peroxidase market. (bccresearch.com)
  • The report delves into the competition landscape of the global horseradish peroxidase market. (bccresearch.com)
  • Key players operating in the global horseradish peroxidase market have been identified, and each one of these has been profiled for distinguishing business attributes. (bccresearch.com)
  • Company overview, financial standings, recent developments, and SWOT are some of the attributes of players in the global horseradish peroxidase market that have been profiled in this report. (bccresearch.com)
  • What is the scope of growth of product companies in the global horseradish peroxidase market? (bccresearch.com)
  • What will be the Y-o-Y growth of the global horseradish peroxidase market between 2020 and 2030? (bccresearch.com)
  • What is the influence of changing trends in life science research on the global horseradish peroxidase market? (bccresearch.com)
  • Which factors are anticipated to hamper the growth of the global horseradish peroxidase market during the forecast period? (bccresearch.com)
  • Which are the leading companies in the global horseradish peroxidase market? (bccresearch.com)
  • A unique research methodology has been utilized by TMR to conduct comprehensive research on the growth of the global horseradish peroxidase market and arrive at conclusions on its growth prospects. (bccresearch.com)
  • Secondary sources referred to by analysts during the production of the global horseradish peroxidase market report include statistics from company annual reports, SEC filings, company websites, investor presentations, regulatory databases, government publications, and industry white papers. (bccresearch.com)
  • Access to an extensive internal repository and external proprietary databases enabled this report to address specific details and questions about the global horseradish peroxidase market with accuracy. (bccresearch.com)
  • This has helped in reaching TMR's estimates on future prospects of the global horseradish peroxidase market more reliably and accurately. (bccresearch.com)
  • Global Horseradish Peroxidase (HRP) Market Trends, Analys. (pharmiweb.com)
  • The report gives the research-based overview of on Global Horseradish Peroxidase (HRP) Market 2019 size, industry status and forecast, competition landscape and growth opportunity. (pharmiweb.com)
  • This research report categorizes the global horseradish peroxidase (hrp) market by companies, region, type and end-use industry. (pharmiweb.com)
  • 1. The primary objective of the study is to understand and gain insights about the global horseradish peroxidase (hrp) market and its segmental analysis by type, by application and by region. (pharmiweb.com)
  • 2. To identify major trends in the global horseradish peroxidase (hrp) market. (pharmiweb.com)
  • 6. To profile major companies operating in the global horseradish peroxidase (hrp) market. (pharmiweb.com)
  • The report offers an in-depth assessment of the global horseradish peroxidase (hrp) market, taking into consideration market competition, regional growth, key portions, and other important features. (pharmiweb.com)
  • The research study is prepared with the use of the latest primary and secondary research methodologies.A research report on the global Horseradish Peroxidase (HRP) market will offer significant estimates for the period between 2019 and 2028. (pharmiweb.com)
  • The global Horseradish Peroxidase (HRP) market report highlights over all the aspects related to the horseradish peroxidase (hrp) market that includes review of the manufactured item, the key growth factors upgrading the market development, application in various sectors, key horseradish peroxidase (hrp) market players. (pharmiweb.com)
  • The report clearly explains the quantitative as well as qualitative nature of the global Horseradish Peroxidase (HRP) market. (pharmiweb.com)
  • Examine in-depth global horseradish peroxidase (hrp) market trends and outlook coupled with the factors driving the market, as well as those hindering it. (pharmiweb.com)
  • The global Horseradish Peroxidase (HRP) market will reach Volume Million USD in 2017 with CAGR xx% 2018-2025. (rnrmarketresearch.com)
  • Global Horseradish Peroxidase (HRP) Market 2021 by Manufacturers, Regions, Type and Application, Forecast to 2026 is a recent research report published by MarketsandResearch.biz that offers a complete research with actual market figures related to the size of the global market in terms of value and volume for the forecast period 2021-2026. (ksusentinel.com)
  • The report shows a comprehensive view of the global Horseradish Peroxidase (HRP) industry to help players to closely understand important transformation within this market. (ksusentinel.com)
  • This report uses various methodological tools to perform a complete study of the global Horseradish Peroxidase (HRP) market. (ksusentinel.com)
  • This research report reveals global Horseradish Peroxidase (HRP) market overview, market share, demand and supply ratio, supply chain analysis, and import/export details. (ksusentinel.com)
  • Numerous substrates for horseradish peroxidase have been described and commercialized to exploit the desirable features of HRP. (wikipedia.org)
  • A total of 22 isoenzymes including allelic variants were successfully expressed in Pichia pastoris and showed peroxidase activity with at least one of the substrates tested, thus enabling their development into commercial pure isoenzymes. (nih.gov)
  • 2004 ). Reduction of peroxides at the expense of electron donating substrates makes peroxidases useful in oxidative breakdown of synthetic azo dyes (Regalado et al. (springeropen.com)
  • Horseradish peroxidase (HRP) is a 40 kDa protein that catalyzes the oxidation of substrates by hydrogen peroxide, resulting in a colored or fluorescent product or release of light as a byproduct of the reaction. (glycomatrix.com)
  • Other substrates which can be used to measure horseradish peroxidase activity include ABTS, TMB and TMBUS. (expedeon.com)
  • Our stabilized ultra-sensitive Luminol based substrates detect horseradish peroxidase at femtogram level and our chemiluminescent systems 3 to 5 more times sensitive than our best competitor. (pjk-gmbh.de)
  • Horseradish peroxidases (HRPs) from Armoracia rusticana have long been utilized as reporters in various diagnostic assays and histochemical stainings. (nih.gov)
  • BPA), p-nonylphenol (p-NP), and p-octylphenol (p-OP) that are known as endocrine disrupters were oxidized by horseradish (Armoracia rusticana) peroxidase (HRP) with H2O2. (semanticscholar.org)
  • Transformed roots of Armoracia rusticana (horseradish), induced by the phytopathogenic soil bacterium Agrobacterium rhizogenes, were cultured in a two litre bubble column reactor with the purpose of characterising their growth and production of horseradish peroxidase. (nrf.ac.za)
  • The worldwide horseradish peroxidase (hrp) market report intensely underlines essential market fragments including types, Key developments, driving commercial centres dependent on topographical regions, key organizations, and innovations. (pharmiweb.com)
  • Worldwide Horseradish Peroxidase (HRP) Market Rising Tren. (pharmiweb.com)
  • High-performance liquid chromatographic investigation of product formation in the horseradish peroxidase-enhanced chemiluminescence of luminol with different enhancers. (rivm.nl)
  • Chemiluminescence flow biosensor for glucose based on gold nanoparticle-enhanced activities of glucose oxidase and horseradish peroxidase. (biomedsearch.com)
  • Glucose oxidase (GOD), horseradish peroxidase (HRP) and gold nanoparticles were immobilized with sol-gel method on the inside surface of the CL flow cell. (biomedsearch.com)
  • Optimising the ratio of horseradish peroxidase and glucose oxidase on " by Dana Mackey, Anthony Killard et al. (dit.ie)
  • Use Peroxidase (POD), Grade II, for the oxidation of reduced dyes in the indicator reaction of many diagnostic tests, e.g., for the determination of blood glucose, triglycerides or lactate. (roche.com)
  • Furthermore, peroxidases are simple and trustworthy way of detecting pathogens, toxins and antigens in various analytes by labeling a pre applied antibody like in ELISA (enzyme immuno sorbent assay) and quantification of chemicals like glucose, lactose, uric acid and cholesterol that are its practical analytical applications in diagnostic kits. (jcebe.org)
  • Horseradish peroxidase (HRP) is the most commonly used enzyme for H 2 O 2 detection because it can oxidize hydrogen atoms and, for example, xenobiotics in the presence of H 2 O 2 . (hindawi.com)
  • horseradish peroxidase enzyme system) for the detection in 96 sera from dogs with blastomycoses (73), other fungal infections (12), and non-infected dogs (11). (thefreedictionary.com)
  • Among them, Streptavidin-containing poly-horseradish peroxidase (PolyHRP) based detection strategies have been shown to improve signals in ELISA. (elsevier.com)
  • Affinity-purified anti-horseradish peroxidase and conjugates are available for detection of horseradish peroxidase antigen or for signal amplification of HRP-containing reagents. (jacksonimmuno.com)
  • TSA-sometimes called CARD, for Catalyzed Reporter Deposition-is an enzyme-mediated detection method that utilizes the catalytic activity of horseradish peroxidase (HRP) to generate high-density labeling of a target protein or nucleic acid sequence in situ . (thermofisher.com)
  • Horseradish peroxidase is ideal in many respects for these applications because it is smaller, more stable, and less expensive than other popular alternatives such as alkaline phosphatase. (wikipedia.org)
  • Horseradish peroxidase is also commonly used in techniques such as ELISA and Immunohistochemistry due to its monomeric nature and the ease with which it produces coloured products. (wikipedia.org)
  • Peroxidase conjugates are commonly used for immunohistochemistry, Western blotting, and ELISA. (jacksonimmuno.com)
  • a peroxidases isolated from horseradish that is used in immunohistochemistry to label the antigen-antibody complex. (drugs.com)
  • Using biotinylated primary or secondary intermediates and peroxidase-labeled Avidin D, antigens can be localized in histological sections, cytospin preparations, or smears. (vectorlabs.com)
  • Peroxidase, a heme-containing oxidoreductase, is a commercially important enzyme which catalyses the reductive cleavage of hydrogen peroxide by an electron donor. (wikipedia.org)
  • Hydrogen peroxide is a mediator of indole-3-acetic acid/horseradish peroxidase-induced apoptosis. (curehunter.com)
  • The chemical nature of the second hydrogen peroxide compound formed by cytochrome c peroxidase and horseradish peroxidase. (biochemj.org)
  • Hydrogen peroxide-dependent covalent binding of phenytoin was observed with thyroid peroxidase and horseradish peroxidase, and binding was significantly reduced in these systems and in PGS and RSV by coincubation with the peroxidase inhibitor methimazole. (aspetjournals.org)
  • Among three concentrations of horseradish peroxidase, the 50000 fold diluted POD (with end concentration of 0.97 μU/ml) showed the best results when consolidated with 44 μM hydrogen peroxide. (nsk.hr)
  • One of the major use of peroxidases are in analytical systems where peroxidase based biosensors are used for organic hydroperoxide and hydrogen peroxide determination. (jcebe.org)
  • A peroxidase isoenzyme secreted by turnip (Brassica napus) hairy-root culture inactivation by hydrogen peroxide and application in diagnostic kits. (jcebe.org)
  • A comparison between the oxidation with laccase and horseradish peroxidase for triclosan conversion. (semanticscholar.org)
  • A comparative study of horseradish peroxidase conjugates prepared with a one-step and a twostep method. (thefreedictionary.com)
  • Horseradish peroxidase (HRP) conjugates are prepared by a modified Nakane and Kawaoi procedure (J. Histochem. (jacksonimmuno.com)
  • Peroxidase conjugates of other targeting proteins such as lectins and receptor ligands are likely to be suitable for labeling targets, as is endogenous peroxidase activity. (thermofisher.com)
  • The radiation-induced inactivation of horseradish peroxidase in dilute aqueous solutions has been studied. (springer.com)
  • Effects of colchicine on endocytosis and cellular inactivation of horseradish peroxidase in cultured chondrocytes. (rupress.org)
  • Horseradish peroxidase (HRP) was used as a marker to study the effects of microtubule-disruptive drugs on uptake and cellular inactivation of exogenous material in cultures of embryonic chick chondrocytes. (rupress.org)
  • Retrograde transport of horseradish peroxidase in sciatic nerve of rats and dystrophy mice. (thefreedictionary.com)
  • Horseradish peroxidase nerve retrograde tracer technique was used to evaluate protective effects on sensory neurons following sciatic nerve defect bridging with tissue engineered artificial nerves constructed by acellular nerve graft and bone marrow mesenchymal stem cells in rats. (ovid.com)
  • Regeneration of sensory neurons in the spinal dorsal root ganglia was assessed using horseradish peroxidase nerve retrograde tracer technique at 12 weeks following surgery. (ovid.com)
  • Horseradish and soybean peroxidases: comparable tools for alternative niches? (dcu.ie)
  • Horseradish and soybean peroxidases (HRP and SBP, respectively) are useful biotechnological tools. (dcu.ie)
  • Besides, playing a potential role by the peroxidases in the soil detoxification, HRP, turnip and soybean peroxidases have also shown their significance in the bioremediation of cresols, phenols and chlorinated phenols contaminated water. (jcebe.org)
  • Other types like manganese peroxidase (MnP) and Lignin Peroxidase (LiP) are of important use in paper industry for performing bio-bleaching and bio-pulping. (jcebe.org)
  • The Zenon Horseradish Peroxidase Antibody Labeling Kits ( Zenon Technology: Versatile Reagents for Immunolabeling-Section 7.3 , Zenon Antibody Labeling Kits-Table 7.7 ), which are described below, are of particular utility when used in combination with TSA technology. (thermofisher.com)
  • 2003) Ectopic expression of a horseradish peroxidase enhances growth rate and increases oxidative stress resistance in hybrid aspen. (thefreedictionary.com)
  • Oxidative degradation of alkylphenols by horseradish peroxidase. (semanticscholar.org)
  • Novel types of aromatic polymers may result from the oxidative polymerization of aromatic amines and phenols by horseradish Peroxidase (HRP) present in water or water-soluble organic solvents. (jcebe.org)
  • Removal of nitroaromatics from synthetic waste water using two-step zero valent ion reduction and peroxidase catalyzed oxidative polymerization. (jcebe.org)
  • The aorta and vena cava of male Sprague-Dawley rats were perfusion-fixed after the intravenous injection of Evans-blue albumin (EBA) or horseradish peroxidase (HRP). (ahajournals.org)
  • Horseradish peroxidase catalyses the oxidation of luminol to 3-aminophthalate via several intermediates. (wikipedia.org)
  • Raknić M. Validation of enhanced luminol-H202-horseradish peroxidase chemiluminescent assay [Master's thesis]. (nsk.hr)
  • The purpose of this study was to establish whether the enhanced luminol-H2O2- horseradish chemiluminescent assay could be used to determine antioxidant activity of reputed antioxidants, ascorbic acid and Trolox. (nsk.hr)
  • Microsomes from hepatic, thyroid, seminal vesicular, or pulmonary tissues, or PGS or horseradish peroxidase, were incubated with the appropriate enzymatic cofactors to study activities of cytochromes P-450 (NADPH), PGS (arachidonic acid), thyroid peroxidase (guiaicol, H2O2), and horseradish peroxidase (H2O2). (aspetjournals.org)
  • Based on this it can be concluded that phenol removal from aqueous solutions greatly depends of peroxidase activity, because this temperature and pH values represents the optimum values of peroxidase enzymatic activity. (stumejournals.com)
  • HRP is often termed the classical plant heme peroxidase and although it has been studied for decades, our understanding has deepened since its cloning and subsequent expression, enabling numerous mutational and protein engineering studies. (dcu.ie)
  • The final paragraphs speculate on the future of plant heme-based peroxidases, with probable trends outlined and explored. (dcu.ie)
  • A seven-proton coupled spin system was identified in the upfield region that is consistent with originating from the proposed catalytic Arg38 residue in the distal heme pocket, with orientation relative to the heme similar to that found in cytochrome c peroxidase. (bionmr.com)
  • It is concluded that 2D NMR studies should be able to provide the heme assignments, aid in identifying the catalytic residues, and provide information on the spatial disposition of such residues in the active site for cyanide complexes of a number of intermediate to large paramagnetic heme peroxidases, as well as for other paramagnetic metalloenzymes with line widths of approximately 100 Hz. (bionmr.com)
  • The MCD spectrum of the acid form of native horseradish peroxidase was characteristic of a ferric high spin heme group. (elsevier.com)
  • Comparison of lignin peroxidase and horseradish peroxidase for catalyzing the removal of nonylphenol from water. (semanticscholar.org)
  • The objectives of this study were to determine the effects of polymer concentration, ultraviolet (UV) light exposure, and film thickness on the activity and stability of horseradish peroxidase (HRP) and alcohol oxidase (AOX) when immobilized in a photo-crosslinkable and water-soluble polymer, poly (vinyl alcohol) azide-unit water pendant (PVA-AWP). (illinois.edu)
  • Nano reengineering of horseradish peroxidase with dendritic macromolecules for stability enhancement. (semanticscholar.org)
  • NMR paper 2D NMR of paramagnetic metalloenzymes: cyanide-inhibited horseradish peroxidase. (bionmr.com)
  • 2D NMR of paramagnetic metalloenzymes: cyanide-inhibited horseradish peroxidase. (bionmr.com)
  • Two-dimensional (2D) proton NMR correlation spectroscopy, COSY, and nuclear Overhauser spectroscopy, NOESY, have been used to explore the applicability of these methods for the moderately large (42 KDa), paramagnetic cyanide-inhibited derivative of horseradish peroxidase, HRP-CN. (bionmr.com)
  • Identification and manual verification of the sequences of 28 HRP isoenzymes do not only contribute a set of peroxidases for industrial, biological and biomedical applications, but also provide valuable information on the reliability of the approach in identifying and characterizing a large group of isoenzymes. (nih.gov)
  • 2) and a mixture of horseradish peroxidase isoenzymes (pI 6. (thefreedictionary.com)
  • Fluorescence microscopic examination of en face preparation of the aorta stained with hematoxylin allowed the identification of endothelial cells that underwent mitosis, together with the localization and quantification of fluorescent spots for EBA leakage. (ahajournals.org)
  • Oxidase-peroxidase reaction: kinetics of peroxidase-catalysed oxidation of 2-aminophenol. (semanticscholar.org)
  • An oxidation-reduction catalyzing enzyme i.e. peroxidases that are produced by numerous plants and microorganism are widely known in today's world. (jcebe.org)
  • Will North America continue to be the most profitable market for horseradish peroxidase providers? (bccresearch.com)
  • The research covers the current market size of the North America Horseradish Peroxidase (HRP) market and its growth rates based on 5 year history data. (medgadget.com)
  • Who are the key vendors in North America Horseradish Peroxidase (HRP) market space? (medgadget.com)
  • What are the key market trends impacting the growth of the North America Horseradish Peroxidase (HRP) market? (medgadget.com)
  • What are the key outcomes of the five forces analysis of the North America Horseradish Peroxidase (HRP) market? (medgadget.com)
  • This report focuses on the horseradish peroxidase (hrp) in the global market, especially in North America, Europe, Asia-Pacific, Latin America, Middle East and Africa . (pharmiweb.com)
  • The enzyme horseradish peroxidase (HRP), found in the roots of horseradish, is used extensively in biochemistry applications. (wikipedia.org)
  • Horseradish peroxidase is isolated from horseradish roots ( Amoracia rusticana ) and belongs to the ferroprotoporphyrin group of peroxidases. (sigmaaldrich.com)
  • Published methods were used to induce and transformed roots from horseradish. (nrf.ac.za)
  • For this, solar-sensitive ZnMe (Me = Al, Cr) layered double hydroxides (LDHs) and their derived mixtures of mixed oxides (MMOs) were synthesized via co-precipitation and calcination at 750 °C, while the calcined LDH at 550 °C was used for horseradish peroxidase (HRP) immobilization through absorption. (springer.com)
  • Immobilization of horseradish peroxidase on NH2-modified magnetic Fe3O4/SiO2 particles and its application in removal of 2,4-dichlorophenol. (sparrho.com)
  • As a substitute for a non-relevant antibody, labelled with peroxidase or an alternative for the goat PAP complex. (nordicmubio.com)
  • Horseradish peroxidase coupled purified goat IgG in phosphate buffered saline (pH7.2). (nordicmubio.com)
  • Horseradish Peroxidase Conjugated Goat Affinity Purified Antibody to Monkey IgG, 1mL antibody storage GENTAUR recommends for long therm storage to freeze at -24 C. For short time storage up to 30 days we suggest fridge storage at 1 to 10 C. Prevent multiple freeze taw cycles of Horseradish Peroxidase Conjugated Goat Affinity Purified Antibody to Monkey IgG, 1mL. (antibody-antibodies.com)
  • Horseradish peroxidase-conjugated IgG fraction of polyclonal Goat antiSerum to Monkey IgG, Fc specific. (exalpha.com)
  • Horseradish peroxidase-coupled purified hyperimmune goat IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2). (exalpha.com)
  • Sequence databases, ORF finding and ORF characterization were utilized to identify peroxidase genes from the 14871 isotigs generated by de novo assembly. (nih.gov)
  • High concentrations of phosphate severely decrease stability of horseradish peroxidase. (wikipedia.org)
  • The isoelectric point for horseradish peroxidase isozymes ranges from 3.0 - 9.0. (sigmaaldrich.com)
  • Changes in peroxidase activity and inperoxidase isozymes in carrot callus. (jcebe.org)
  • Peroxidase, polyphenol oxidase and shikimate dehydrogenase isozymes in relation to the tissue type, maturity and pathogen induction of watermelon seedlings. (jcebe.org)
  • Cochlear vessel permeability to horseradish peroxidase in the normal and acoustically traumatized chinchilla: A reevaluation. (thefreedictionary.com)