Antigens determined by leukocyte loci found on chromosome 6, the major histocompatibility loci in humans. They are polypeptides or glycoproteins found on most nucleated cells and platelets, determine tissue types for transplantation, and are associated with certain diseases.
Substances that are recognized by the immune system and induce an immune reaction.
A specific HLA-B surface antigen subtype. Members of this subtype contain alpha chains that are encoded by the HLA-B*27 allele family.
A specific HLA-B surface antigen subtype. Members of this subtype contain alpha chains that are encoded by the HLA-B*44 allele family.
Class I human histocompatibility (HLA) surface antigens encoded by more than 30 detectable alleles on locus B of the HLA complex, the most polymorphic of all the HLA specificities. Several of these antigens (e.g., HLA-B27, -B7, -B8) are strongly associated with predisposition to rheumatoid and other autoimmune disorders. Like other class I HLA determinants, they are involved in the cellular immune reactivity of cytolytic T lymphocytes.
Substances elaborated by bacteria that have antigenic activity.
Proteins, glycoprotein, or lipoprotein moieties on surfaces of tumor cells that are usually identified by monoclonal antibodies. Many of these are of either embryonic or viral origin.
Antigens on surfaces of cells, including infectious or foreign cells or viruses. They are usually protein-containing groups on cell membranes or walls and may be isolated.
Substances elaborated by viruses that have antigenic activity.
A specific HLA-B surface antigen subtype. Members of this subtype contain alpha chains that are encoded by the HLA-B*08 allele family.
A specific HLA-B surface antigen subtype. Members of this subtype contain alpha chains that are encoded by the HLA-B*37 allele family.
A chronic inflammatory condition affecting the axial joints, such as the SACROILIAC JOINT and other intervertebral or costovertebral joints. It occurs predominantly in young males and is characterized by pain and stiffness of joints (ANKYLOSIS) with inflammation at tendon insertions.
An aseptic, inflammatory arthritis developing secondary to a primary extra-articular infection, most typically of the GASTROINTESTINAL TRACT or UROGENITAL SYSTEM. The initiating trigger pathogens are usually SHIGELLA; SALMONELLA; YERSINIA; CAMPYLOBACTER; or CHLAMYDIA TRACHOMATIS. Reactive arthritis is strongly associated with HLA-B27 ANTIGEN.
Polymorphic class I human histocompatibility (HLA) surface antigens present on almost all nucleated cells. At least 20 antigens have been identified which are encoded by the A locus of multiple alleles on chromosome 6. They serve as targets for T-cell cytolytic responses and are involved with acceptance or rejection of tissue/organ grafts.
Any part or derivative of any protozoan that elicits immunity; malaria (Plasmodium) and trypanosome antigens are presently the most frequently encountered.
A subclass of HLA-D antigens that consist of alpha and beta chains. The inheritance of HLA-DR antigens differs from that of the HLA-DQ ANTIGENS and HLA-DP ANTIGENS.
Inflammation of the anterior uvea comprising the iris, angle structures, and the ciliary body. Manifestations of this disorder include ciliary injection, exudation into the anterior chamber, iris changes, and adhesions between the iris and lens (posterior synechiae). Intraocular pressure may be increased or reduced.
Polyomavirus antigens which cause infection and cellular transformation. The large T antigen is necessary for the initiation of viral DNA synthesis, repression of transcription of the early region and is responsible in conjunction with the middle T antigen for the transformation of primary cells. Small T antigen is necessary for the completion of the productive infection cycle.
Sites on an antigen that interact with specific antibodies.
A specific HLA-B surface antigen subtype. Members of this subtype contain alpha chains that are encoded by the HLA-B*35 allele family.
Substances of fungal origin that have antigenic activity.
Differentiation antigens residing on mammalian leukocytes. CD stands for cluster of differentiation, which refers to groups of monoclonal antibodies that show similar reactivity with certain subpopulations of antigens of a particular lineage or differentiation stage. The subpopulations of antigens are also known by the same CD designation.
Identification of the major histocompatibility antigens of transplant DONORS and potential recipients, usually by serological tests. Donor and recipient pairs should be of identical ABO blood group, and in addition should be matched as closely as possible for HISTOCOMPATIBILITY ANTIGENS in order to minimize the likelihood of allograft rejection. (King, Dictionary of Genetics, 4th ed)
The immovable joint formed by the lateral surfaces of the SACRUM and ILIUM.
Any part or derivative of a helminth that elicits an immune reaction. The most commonly seen helminth antigens are those of the schistosomes.
The major group of transplantation antigens in the mouse.
A specific HLA-B surface antigen subtype. Members of this subtype contain alpha chains that are encoded by the HLA-B*07 allele family.
Class I human histocompatibility (HLA) antigens encoded by a small cluster of structural genes at the C locus on chromosome 6. They have significantly lower immunogenicity than the HLA-A and -B determinants and are therefore of minor importance in donor/recipient crossmatching. Their primary role is their high-risk association with certain disease manifestations (e.g., spondylarthritis, psoriasis, multiple myeloma).
Heterogeneous group of arthritic diseases sharing clinical and radiologic features. They are associated with the HLA-B27 ANTIGEN and some with a triggering infection. Most involve the axial joints in the SPINE, particularly the SACROILIAC JOINT, but can also involve asymmetric peripheral joints. Subsets include ANKYLOSING SPONDYLITIS; REACTIVE ARTHRITIS; PSORIATIC ARTHRITIS; and others.
Large, transmembrane, non-covalently linked glycoproteins (alpha and beta). Both chains can be polymorphic although there is more structural variation in the beta chains. The class II antigens in humans are called HLA-D ANTIGENS and are coded by a gene on chromosome 6. In mice, two genes named IA and IE on chromosome 17 code for the H-2 antigens. The antigens are found on B-lymphocytes, macrophages, epidermal cells, and sperm and are thought to mediate the competence of and cellular cooperation in the immune response. The term IA antigens used to refer only to the proteins encoded by the IA genes in the mouse, but is now used as a generic term for any class II histocompatibility antigen.
Membrane glycoproteins consisting of an alpha subunit and a BETA 2-MICROGLOBULIN beta subunit. In humans, highly polymorphic genes on CHROMOSOME 6 encode the alpha subunits of class I antigens and play an important role in determining the serological specificity of the surface antigen. Class I antigens are found on most nucleated cells and are generally detected by their reactivity with alloantisera. These antigens are recognized during GRAFT REJECTION and restrict cell-mediated lysis of virus-infected cells.
A glycoprotein that is secreted into the luminal surface of the epithelia in the gastrointestinal tract. It is found in the feces and pancreaticobiliary secretions and is used to monitor the response to colon cancer treatment.
Serological reactions in which an antiserum against one antigen reacts with a non-identical but closely related antigen.
Those proteins recognized by antibodies from serum of animals bearing tumors induced by viruses; these proteins are presumably coded for by the nucleic acids of the same viruses that caused the neoplastic transformation.
A group of antigens that includes both the major and minor histocompatibility antigens. The former are genetically determined by the major histocompatibility complex. They determine tissue type for transplantation and cause allograft rejections. The latter are systems of allelic alloantigens that can cause weak transplant rejection.
A group of the D-related HLA antigens found to differ from the DR antigens in genetic locus and therefore inheritance. These antigens are polymorphic glycoproteins comprising alpha and beta chains and are found on lymphoid and other cells, often associated with certain diseases.
Molecules on the surface of T-lymphocytes that recognize and combine with antigens. The receptors are non-covalently associated with a complex of several polypeptides collectively called CD3 antigens (ANTIGENS, CD3). Recognition of foreign antigen and the major histocompatibility complex is accomplished by a single heterodimeric antigen-receptor structure, composed of either alpha-beta (RECEPTORS, ANTIGEN, T-CELL, ALPHA-BETA) or gamma-delta (RECEPTORS, ANTIGEN, T-CELL, GAMMA-DELTA) chains.
A technique to study CELL MIGRATION in the INFLAMMATION process or during immune reactions. After an area on the skin is abraded, the movement of cells in the area is followed via microscopic observation of the exudate through a coverslip or tissue culture chamber placed over the area.
Antibodies produced by a single clone of cells.
A specific HLA-A surface antigen subtype. Members of this subtype contain alpha chains that are encoded by the HLA-A*02 allele family.
A specific HLA-B surface antigen subtype. Members of this subtype contain alpha chains that are encoded by the HLA-B*51 allele family.
Immunized T-lymphocytes which can directly destroy appropriate target cells. These cytotoxic lymphocytes may be generated in vitro in mixed lymphocyte cultures (MLC), in vivo during a graft-versus-host (GVH) reaction, or after immunization with an allograft, tumor cell or virally transformed or chemically modified target cell. The lytic phenomenon is sometimes referred to as cell-mediated lympholysis (CML). These CD8-positive cells are distinct from NATURAL KILLER CELLS and NATURAL KILLER T-CELLS. There are two effector phenotypes: TC1 and TC2.
Human immune-response or Class II antigens found mainly, but not exclusively, on B-lymphocytes and produced from genes of the HLA-D locus. They are extremely polymorphic families of glycopeptides, each consisting of two chains, alpha and beta. This group of antigens includes the -DR, -DQ and -DP designations, of which HLA-DR is most studied; some of these glycoproteins are associated with certain diseases, possibly of immune etiology.
Nuclear antigen with a role in DNA synthesis, DNA repair, and cell cycle progression. PCNA is required for the coordinated synthesis of both leading and lagging strands at the replication fork during DNA replication. PCNA expression correlates with the proliferation activity of several malignant and non-malignant cell types.
Persons who have experienced prolonged survival of HIV infection. This includes the full spectrum of untreated, HIV-infected long-term asymptomatics to those with AIDS who have survived due to successful treatment.
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
Lymphocytes responsible for cell-mediated immunity. Two types have been identified - cytotoxic (T-LYMPHOCYTES, CYTOTOXIC) and helper T-lymphocytes (T-LYMPHOCYTES, HELPER-INDUCER). They are formed when lymphocytes circulate through the THYMUS GLAND and differentiate to thymocytes. When exposed to an antigen, they divide rapidly and produce large numbers of new T cells sensitized to that antigen.
The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.
IMMUNOGLOBULINS on the surface of B-LYMPHOCYTES. Their MESSENGER RNA contains an EXON with a membrane spanning sequence, producing immunoglobulins in the form of type I transmembrane proteins as opposed to secreted immunoglobulins (ANTIBODIES) which do not contain the membrane spanning segment.
A glycoprotein that is a kallikrein-like serine proteinase and an esterase, produced by epithelial cells of both normal and malignant prostate tissue. It is an important marker for the diagnosis of prostate cancer.
The lipopolysaccharide-protein somatic antigens, usually from gram-negative bacteria, important in the serological classification of enteric bacilli. The O-specific chains determine the specificity of the O antigens of a given serotype. O antigens are the immunodominant part of the lipopolysaccharide molecule in the intact bacterial cell. (From Singleton & Sainsbury, Dictionary of Microbiology and Molecular Biology, 2d ed)
Arthritis caused by BACTERIA; RICKETTSIA; MYCOPLASMA; VIRUSES; FUNGI; or PARASITES.
Variant forms of the same gene, occupying the same locus on homologous CHROMOSOMES, and governing the variants in production of the same gene product.
Differentiation antigens found on thymocytes and on cytotoxic and suppressor T-lymphocytes. CD8 antigens are members of the immunoglobulin supergene family and are associative recognition elements in MHC (Major Histocompatibility Complex) Class I-restricted interactions.
A trisaccharide antigen expressed on glycolipids and many cell-surface glycoproteins. In the blood the antigen is found on the surface of NEUTROPHILS; EOSINOPHILS; and MONOCYTES. In addition, CD15 antigen is a stage-specific embryonic antigen.
Immunoglobulins produced in a response to BACTERIAL ANTIGENS.
Carbohydrate antigens expressed by malignant tissue. They are useful as tumor markers and are measured in the serum by means of a radioimmunoassay employing monoclonal antibodies.
An immunoassay utilizing an antibody labeled with an enzyme marker such as horseradish peroxidase. While either the enzyme or the antibody is bound to an immunosorbent substrate, they both retain their biologic activity; the change in enzyme activity as a result of the enzyme-antibody-antigen reaction is proportional to the concentration of the antigen and can be measured spectrophotometrically or with the naked eye. Many variations of the method have been developed.
A transient increase in the number of leukocytes in a body fluid.
A critical subpopulation of regulatory T-lymphocytes involved in MHC Class I-restricted interactions. They include both cytotoxic T-lymphocytes (T-LYMPHOCYTES, CYTOTOXIC) and CD8+ suppressor T-lymphocytes.
Antigenic determinants recognized and bound by the T-cell receptor. Epitopes recognized by the T-cell receptor are often located in the inner, unexposed side of the antigen, and become accessible to the T-cell receptors after proteolytic processing of the antigen.
Complex of at least five membrane-bound polypeptides in mature T-lymphocytes that are non-covalently associated with one another and with the T-cell receptor (RECEPTORS, ANTIGEN, T-CELL). The CD3 complex includes the gamma, delta, epsilon, zeta, and eta chains (subunits). When antigen binds to the T-cell receptor, the CD3 complex transduces the activating signals to the cytoplasm of the T-cell. The CD3 gamma and delta chains (subunits) are separate from and not related to the gamma/delta chains of the T-cell receptor (RECEPTORS, ANTIGEN, T-CELL, GAMMA-DELTA).
Those hepatitis B antigens found on the surface of the Dane particle and on the 20 nm spherical and tubular particles. Several subspecificities of the surface antigen are known. These were formerly called the Australia antigen.
Sets of cell surface antigens located on BLOOD CELLS. They are usually membrane GLYCOPROTEINS or GLYCOLIPIDS that are antigenically distinguished by their carbohydrate moieties.
Morphologic alteration of small B LYMPHOCYTES or T LYMPHOCYTES in culture into large blast-like cells able to synthesize DNA and RNA and to divide mitotically. It is induced by INTERLEUKINS; MITOGENS such as PHYTOHEMAGGLUTININS, and by specific ANTIGENS. It may also occur in vivo as in GRAFT REJECTION.
Gram-negative, non-motile, capsulated, gas-producing rods found widely in nature and associated with urinary and respiratory infections in humans.
The property of antibodies which enables them to react with some ANTIGENIC DETERMINANTS and not with others. Specificity is dependent on chemical composition, physical forces, and molecular structure at the binding site.
The major interferon produced by mitogenically or antigenically stimulated LYMPHOCYTES. It is structurally different from TYPE I INTERFERON and its major activity is immunoregulation. It has been implicated in the expression of CLASS II HISTOCOMPATIBILITY ANTIGENS in cells that do not normally produce them, leading to AUTOIMMUNE DISEASES.
Antibodies that react with self-antigens (AUTOANTIGENS) of the organism that produced them.
55-kDa antigens found on HELPER-INDUCER T-LYMPHOCYTES and on a variety of other immune cell types. CD4 antigens are members of the immunoglobulin supergene family and are implicated as associative recognition elements in MAJOR HISTOCOMPATIBILITY COMPLEX class II-restricted immune responses. On T-lymphocytes they define the helper/inducer subset. CD4 antigens also serve as INTERLEUKIN-15 receptors and bind to the HIV receptors, binding directly to the HIV ENVELOPE PROTEIN GP120.
High-molecular weight glycoproteins uniquely expressed on the surface of LEUKOCYTES and their hemopoietic progenitors. They contain a cytoplasmic protein tyrosine phosphatase activity which plays a role in intracellular signaling from the CELL SURFACE RECEPTORS. The CD45 antigens occur as multiple isoforms that result from alternative mRNA splicing and differential usage of three exons.
Established cell cultures that have the potential to propagate indefinitely.
T-cell receptors composed of CD3-associated alpha and beta polypeptide chains and expressed primarily in CD4+ or CD8+ T-cells. Unlike immunoglobulins, the alpha-beta T-cell receptors recognize antigens only when presented in association with major histocompatibility (MHC) molecules.
Molecules on the surface of B- and T-lymphocytes that recognize and combine with specific antigens.
Antigens of the virion of the HEPATITIS B VIRUS or the Dane particle, its surface (HEPATITIS B SURFACE ANTIGENS), core (HEPATITIS B CORE ANTIGENS), and other associated antigens, including the HEPATITIS B E ANTIGENS.
Transmembrane proteins that form the beta subunits of the HLA-DQ antigens.
Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
The processes triggered by interactions of ANTIBODIES with their ANTIGENS.
A subtype of HLA-DRB beta chains that includes over one hundred allele variants. The HLA-DRB1 subtype is associated with several of the HLA-DR SEROLOGICAL SUBTYPES.
The production of ANTIBODIES by proliferating and differentiated B-LYMPHOCYTES under stimulation by ANTIGENS.
Glycoproteins expressed on cortical thymocytes and on some dendritic cells and B-cells. Their structure is similar to that of MHC Class I and their function has been postulated as similar also. CD1 antigens are highly specific markers for human LANGERHANS CELLS.
Lymphoid cells concerned with humoral immunity. They are short-lived cells resembling bursa-derived lymphocytes of birds in their production of immunoglobulin upon appropriate stimulation.
An HLA-DR antigen which is associated with HLA-DRB1 CHAINS encoded by DRB1*03 alleles.
Serum that contains antibodies. It is obtained from an animal that has been immunized either by ANTIGEN injection or infection with microorganisms containing the antigen.
Deliberate stimulation of the host's immune response. ACTIVE IMMUNIZATION involves administration of ANTIGENS or IMMUNOLOGIC ADJUVANTS. PASSIVE IMMUNIZATION involves administration of IMMUNE SERA or LYMPHOCYTES or their extracts (e.g., transfer factor, immune RNA) or transplantation of immunocompetent cell producing tissue (thymus or bone marrow).
Antigens expressed primarily on the membranes of living cells during sequential stages of maturation and differentiation. As immunologic markers they have high organ and tissue specificity and are useful as probes in studies of normal cell development as well as neoplastic transformation.
An HLA-DR antigen which is associated with HLA-DRB1 CHAINS encoded by DRB1*04 alleles.
Members of the class of compounds composed of AMINO ACIDS joined together by peptide bonds between adjacent amino acids into linear, branched or cyclical structures. OLIGOPEPTIDES are composed of approximately 2-12 amino acids. Polypeptides are composed of approximately 13 or more amino acids. PROTEINS are linear polypeptides that are normally synthesized on RIBOSOMES.
A species of the genus YERSINIA, isolated from both man and animal. It is a frequent cause of bacterial gastroenteritis in children.
Genetic loci in the vertebrate major histocompatibility complex that encode polymorphic products which control the immune response to specific antigens. The genes are found in the HLA-D region in humans and in the I region in mice.
Antigens associated with specific proteins of the human adult T-cell immunodeficiency virus (HIV); also called HTLV-III-associated and lymphadenopathy-associated virus (LAV) antigens.
A specific HLA-A surface antigen subtype. Members of this subtype contain alpha chains that are encoded by the HLA-A*01 allele family.
A melanosome-specific protein that plays a role in the expression, stability, trafficking, and processing of GP100 MELANOMA ANTIGEN, which is critical to the formation of Stage II MELANOSOMES. The protein is used as an antigen marker for MELANOMA cells.
The genetic region which contains the loci of genes which determine the structure of the serologically defined (SD) and lymphocyte-defined (LD) TRANSPLANTATION ANTIGENS, genes which control the structure of the IMMUNE RESPONSE-ASSOCIATED ANTIGENS, HUMAN; the IMMUNE RESPONSE GENES which control the ability of an animal to respond immunologically to antigenic stimuli, and genes which determine the structure and/or level of the first four components of complement.
A costimulatory ligand expressed by ANTIGEN-PRESENTING CELLS that binds to CTLA-4 ANTIGEN with high specificity and to CD28 ANTIGEN with low specificity. The interaction of CD80 with CD28 ANTIGEN provides a costimulatory signal to T-LYMPHOCYTES, while its interaction with CTLA-4 ANTIGEN may play a role in inducing PERIPHERAL TOLERANCE.
A subspecialty of internal medicine concerned with the study of inflammatory or degenerative processes and metabolic derangement of connective tissue structures which pertain to a variety of musculoskeletal disorders, such as arthritis.
Immunologic techniques based on the use of: (1) enzyme-antibody conjugates; (2) enzyme-antigen conjugates; (3) antienzyme antibody followed by its homologous enzyme; or (4) enzyme-antienzyme complexes. These are used histologically for visualizing or labeling tissue specimens.
Represents 15-20% of the human serum immunoglobulins, mostly as the 4-chain polymer in humans or dimer in other mammals. Secretory IgA (IMMUNOGLOBULIN A, SECRETORY) is the main immunoglobulin in secretions.
Nuclear antigens encoded by VIRAL GENES found in HUMAN HERPESVIRUS 4. At least six nuclear antigens have been identified.
Technique using an instrument system for making, processing, and displaying one or more measurements on individual cells obtained from a cell suspension. Cells are usually stained with one or more fluorescent dyes specific to cell components of interest, e.g., DNA, and fluorescence of each cell is measured as it rapidly transverses the excitation beam (laser or mercury arc lamp). Fluorescence provides a quantitative measure of various biochemical and biophysical properties of the cell, as well as a basis for cell sorting. Other measurable optical parameters include light absorption and light scattering, the latter being applicable to the measurement of cell size, shape, density, granularity, and stain uptake.
Immunoglobulin molecules having a specific amino acid sequence by virtue of which they interact only with the ANTIGEN (or a very similar shape) that induced their synthesis in cells of the lymphoid series (especially PLASMA CELLS).
Endogenous tissue constituents that have the ability to interact with AUTOANTIBODIES and cause an immune response.
Genetic loci in the vertebrate major histocompatibility complex which encode polymorphic characteristics not related to immune responsiveness or complement activity, e.g., B loci (chicken), DLA (dog), GPLA (guinea pig), H-2 (mouse), RT-1 (rat), HLA-A, -B, and -C class I genes of man.
Antigens stimulating the formation of, or combining with heterophile antibodies. They are cross-reacting antigens found in phylogenetically unrelated species.
Differentiation antigens expressed on B-lymphocytes and B-cell precursors. They are involved in regulation of B-cell proliferation.
The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.
A heterogeneous group of immunocompetent cells that mediate the cellular immune response by processing and presenting antigens to the T-cells. Traditional antigen-presenting cells include MACROPHAGES; DENDRITIC CELLS; LANGERHANS CELLS; and B-LYMPHOCYTES. FOLLICULAR DENDRITIC CELLS are not traditional antigen-presenting cells, but because they hold antigen on their cell surface in the form of IMMUNE COMPLEXES for B-cell recognition they are considered so by some authors.
Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others.
The hepatitis B antigen within the core of the Dane particle, the infectious hepatitis virion.
A genus of the family BACULOVIRIDAE, subfamily Eubaculovirinae, characterized by ovicylindrical occlusion bodies. The type species is Cydia pomonella granulovirus.
A member of the tumor necrosis factor receptor superfamily with specificity for CD40 LIGAND. It is found on mature B-LYMPHOCYTES and some EPITHELIAL CELLS, lymphoid DENDRITIC CELLS. Evidence suggests that CD40-dependent activation of B-cells is important for generation of memory B-cells within the germinal centers. Mutations of the gene for CD40 antigen result in HYPER-IGM IMMUNODEFICIENCY SYNDROME, TYPE 3. Signaling of the receptor occurs through its association with TNF RECEPTOR-ASSOCIATED FACTORS.
A class of immunoglobulin bearing mu chains (IMMUNOGLOBULIN MU-CHAINS). IgM can fix COMPLEMENT. The name comes from its high molecular weight and originally being called a macroglobulin.
A critical subpopulation of T-lymphocytes involved in the induction of most immunological functions. The HIV virus has selective tropism for the T4 cell which expresses the CD4 phenotypic marker, a receptor for HIV. In fact, the key element in the profound immunosuppression seen in HIV infection is the depletion of this subset of T-lymphocytes.
Infections with bacteria of the genus SALMONELLA.
Antibodies from an individual that react with ISOANTIGENS of another individual of the same species.
A sex-specific cell surface antigen produced by the sex-determining gene of the Y chromosome in mammals. It causes syngeneic grafts from males to females to be rejected and interacts with somatic elements of the embryologic undifferentiated gonad to produce testicular organogenesis.
The complex formed by the binding of antigen and antibody molecules. The deposition of large antigen-antibody complexes leading to tissue damage causes IMMUNE COMPLEX DISEASES.
Technique involving the diffusion of antigen or antibody through a semisolid medium, usually agar or agarose gel, with the result being a precipitin reaction.
Proteins conjugated with nucleic acids.
Manifestations of the immune response which are mediated by antigen-sensitized T-lymphocytes via lymphokines or direct cytotoxicity. This takes place in the absence of circulating antibody or where antibody plays a subordinate role.
The sum of the weight of all the atoms in a molecule.
A group of differentiation surface antigens, among the first to be discovered on thymocytes and T-lymphocytes. Originally identified in the mouse, they are also found in other species including humans, and are expressed on brain neurons and other cells.
White blood cells formed in the body's lymphoid tissue. The nucleus is round or ovoid with coarse, irregularly clumped chromatin while the cytoplasm is typically pale blue with azurophilic (if any) granules. Most lymphocytes can be classified as either T or B (with subpopulations of each), or NATURAL KILLER CELLS.
A glycolipid, cross-species antigen that induces production of antisheep hemolysin. It is present on the tissue cells of many species but absent in humans. It is found in many infectious agents.
A costimulatory ligand expressed by ANTIGEN-PRESENTING CELLS that binds to CD28 ANTIGEN with high specificity and to CTLA-4 ANTIGEN with low specificity. The interaction of CD86 with CD28 ANTIGEN provides a stimulatory signal to T-LYMPHOCYTES, while its interaction with CTLA-4 ANTIGEN may play a role in inducing PERIPHERAL TOLERANCE.
A group of genetically identical cells all descended from a single common ancestral cell by mitosis in eukaryotes or by binary fission in prokaryotes. Clone cells also include populations of recombinant DNA molecules all carrying the same inserted sequence. (From King & Stansfield, Dictionary of Genetics, 4th ed)
Serologic tests based on inactivation of complement by the antigen-antibody complex (stage 1). Binding of free complement can be visualized by addition of a second antigen-antibody system such as red cells and appropriate red cell antibody (hemolysin) requiring complement for its completion (stage 2). Failure of the red cells to lyse indicates that a specific antigen-antibody reaction has taken place in stage 1. If red cells lyse, free complement is present indicating no antigen-antibody reaction occurred in stage 1.
Time period from 2001 through 2100 of the common era.
A specific HLA-A surface antigen subtype. Members of this subtype contain alpha chains that are encoded by the HLA-A*03 allele family.
An inhibitory T CELL receptor that is closely related to CD28 ANTIGEN. It has specificity for CD80 ANTIGEN and CD86 ANTIGEN and acts as a negative regulator of peripheral T cell function. CTLA-4 antigen is believed to play role in inducing PERIPHERAL TOLERANCE.
A bacterium which is one of the etiologic agents of bacillary dysentery (DYSENTERY, BACILLARY) and sometimes of infantile gastroenteritis.
Specialized cells of the hematopoietic system that have branch-like extensions. They are found throughout the lymphatic system, and in non-lymphoid tissues such as SKIN and the epithelia of the intestinal, respiratory, and reproductive tracts. They trap and process ANTIGENS, and present them to T-CELLS, thereby stimulating CELL-MEDIATED IMMUNITY. They are different from the non-hematopoietic FOLLICULAR DENDRITIC CELLS, which have a similar morphology and immune system function, but with respect to humoral immunity (ANTIBODY PRODUCTION).
An albumin obtained from the white of eggs. It is a member of the serpin superfamily.
A species of POLYOMAVIRUS originally isolated from Rhesus monkey kidney tissue. It produces malignancy in human and newborn hamster kidney cell cultures.
A specific HLA-A surface antigen subtype. Members of this subtype contain alpha chains that are encoded by the HLA-A*24 allele family.
Immunoglobulins produced in response to VIRAL ANTIGENS.
Proteins prepared by recombinant DNA technology.
A broad specificity HLA-DR antigen that is associated with HLA-DRB1 CHAINS encoded by DRB1*01:15 and DRB1*01:16 alleles.
The species Oryctolagus cuniculus, in the family Leporidae, order LAGOMORPHA. Rabbits are born in burrows, furless, and with eyes and ears closed. In contrast with HARES, rabbits have 22 chromosome pairs.
A melanosome-associated protein that plays a role in the maturation of the MELANOSOME.
The type species of LENTIVIRUS and the etiologic agent of AIDS. It is characterized by its cytopathic effect and affinity for the T4-lymphocyte.
A technique that combines protein electrophoresis and double immunodiffusion. In this procedure proteins are first separated by gel electrophoresis (usually agarose), then made visible by immunodiffusion of specific antibodies. A distinct elliptical precipitin arc results for each protein detectable by the antisera.
A HLA-DR antigen that is associated with HLA-DRB1 CHAINS encoded by DRB1*07 alleles.
The specific failure of a normally responsive individual to make an immune response to a known antigen. It results from previous contact with the antigen by an immunologically immature individual (fetus or neonate) or by an adult exposed to extreme high-dose or low-dose antigen, or by exposure to radiation, antimetabolites, antilymphocytic serum, etc.
Immunoglobulins produced in a response to PROTOZOAN ANTIGENS.
Diagnostic procedures involving immunoglobulin reactions.
Allelic alloantigens often responsible for weak graft rejection in cases when (major) histocompatibility has been established by standard tests. In the mouse they are coded by more than 500 genes at up to 30 minor histocompatibility loci. The most well-known minor histocompatibility antigen in mammals is the H-Y antigen.
A component of the B-cell antigen receptor that is involved in B-cell antigen receptor heavy chain transport to the PLASMA MEMBRANE. It is expressed almost exclusively in B-LYMPHOCYTES and serves as a useful marker for B-cell NEOPLASMS.
A broad-specificity HLA-DR antigen that is associated with HLA-DRB1 CHAINS encoded by DRB1*11 and DRB1*12 alleles.
Antigens that exist in alternative (allelic) forms in a single species. When an isoantigen is encountered by species members who lack it, an immune response is induced. Typical isoantigens are the BLOOD GROUP ANTIGENS.
Sialylated Lewis blood group carbohydrate antigen found in many adenocarcinomas of the digestive tract, especially pancreatic tumors.
Binary classification measures to assess test results. Sensitivity or recall rate is the proportion of true positives. Specificity is the probability of correctly determining the absence of a condition. (From Last, Dictionary of Epidemiology, 2d ed)
Histochemical localization of immunoreactive substances using labeled antibodies as reagents.
The genetic constitution of individuals with respect to one member of a pair of allelic genes, or sets of genes that are closely linked and tend to be inherited together such as those of the MAJOR HISTOCOMPATIBILITY COMPLEX.
Genetically identical individuals developed from brother and sister matings which have been carried out for twenty or more generations, or by parent x offspring matings carried out with certain restrictions. All animals within an inbred strain trace back to a common ancestor in the twentieth generation.
Sensitive tests to measure certain antigens, antibodies, or viruses, using their ability to agglutinate certain erythrocytes. (From Stedman, 26th ed)
A tumor necrosis factor receptor subtype found in a variety of tissues and on activated LYMPHOCYTES. It has specificity for FAS LIGAND and plays a role in regulation of peripheral immune responses and APOPTOSIS. Multiple isoforms of the protein exist due to multiple ALTERNATIVE SPLICING. The activated receptor signals via a conserved death domain that associates with specific TNF RECEPTOR-ASSOCIATED FACTORS in the CYTOPLASM.
A group of dominantly and independently inherited antigens associated with the ABO blood factors. They are glycolipids present in plasma and secretions that may adhere to the erythrocytes. The phenotype Le(b) is the result of the interaction of the Le gene Le(a) with the genes for the ABO blood groups.
The degree of antigenic similarity between the tissues of different individuals, which determines the acceptance or rejection of allografts.
Electrophoresis in which a polyacrylamide gel is used as the diffusion medium.
A CELL CYCLE and tumor growth marker which can be readily detected using IMMUNOCYTOCHEMISTRY methods. Ki-67 is a nuclear antigen present only in the nuclei of cycling cells.
A closely related group of antigens found in the plasma only during the infective phase of hepatitis B or in virulent chronic hepatitis B, probably indicating active virus replication; there are three subtypes which may exist in a complex with immunoglobulins G.
Immunoglobulins produced in a response to HELMINTH ANTIGENS.
Cells artificially created by fusion of activated lymphocytes with neoplastic cells. The resulting hybrid cells are cloned and produce pure MONOCLONAL ANTIBODIES or T-cell products, identical to those produced by the immunologically competent parent cell.
The demonstration of the cytotoxic effect on a target cell of a lymphocyte, a mediator released by a sensitized lymphocyte, an antibody, or complement.
Class I human histocompatibility (HLA) surface antigens encoded by alleles on locus B of the HLA complex. The HLA-G antigens are considered non-classical class I antigens due to their distinct tissue distribution which differs from HLA-A; HLA-B; and HLA-C antigens. Note that several isoforms of HLA-G antigens result from alternative splicing of messenger RNAs produced from the HLA-G*01 allele.
Cells grown in vitro from neoplastic tissue. If they can be established as a TUMOR CELL LINE, they can be propagated in cell culture indefinitely.
Costimulatory T-LYMPHOCYTE receptors that have specificity for CD80 ANTIGEN and CD86 ANTIGEN. Activation of this receptor results in increased T-cell proliferation, cytokine production and promotion of T-cell survival.
Antigens which may directly stimulate B lymphocytes without the cooperation of T lymphocytes.
Glycoprotein members of the immunoglobulin superfamily which participate in T-cell adhesion and activation. They are expressed on most peripheral T-lymphocytes, natural killer cells, and thymocytes, and function as co-receptors or accessory molecules in the T-cell receptor complex.
Glycoproteins found on the membrane or surface of cells.
Transmembrane proteins that form the alpha subunits of the HLA-DQ antigens.
A malignant neoplasm derived from cells that are capable of forming melanin, which may occur in the skin of any part of the body, in the eye, or, rarely, in the mucous membranes of the genitalia, anus, oral cavity, or other sites. It occurs mostly in adults and may originate de novo or from a pigmented nevus or malignant lentigo. Melanomas frequently metastasize widely, and the regional lymph nodes, liver, lungs, and brain are likely to be involved. The incidence of malignant skin melanomas is rising rapidly in all parts of the world. (Stedman, 25th ed; from Rook et al., Textbook of Dermatology, 4th ed, p2445)
The major human blood type system which depends on the presence or absence of two antigens A and B. Type O occurs when neither A nor B is present and AB when both are present. A and B are genetic factors that determine the presence of enzymes for the synthesis of certain glycoproteins mainly in the red cell membrane.
An increased reactivity to specific antigens mediated not by antibodies but by cells.
A technique using antibodies for identifying or quantifying a substance. Usually the substance being studied serves as antigen both in antibody production and in measurement of antibody by the test substance.
Carbohydrate antigen most commonly seen in tumors of the ovary and occasionally seen in breast, kidney, and gastrointestinal tract tumors and normal tissue. CA 125 is clearly tumor-associated but not tumor-specific.
Immunologically detectable substances found in the CELL NUCLEUS.
In vitro method for producing large amounts of specific DNA or RNA fragments of defined length and sequence from small amounts of short oligonucleotide flanking sequences (primers). The essential steps include thermal denaturation of the double-stranded target molecules, annealing of the primers to their complementary sequences, and extension of the annealed primers by enzymatic synthesis with DNA polymerase. The reaction is efficient, specific, and extremely sensitive. Uses for the reaction include disease diagnosis, detection of difficult-to-isolate pathogens, mutation analysis, genetic testing, DNA sequencing, and analyzing evolutionary relationships.
The sequential correspondence of nucleotides in one nucleic acid molecule with those of another nucleic acid molecule. Sequence homology is an indication of the genetic relatedness of different organisms and gene function.
The type species of LYMPHOCRYPTOVIRUS, subfamily GAMMAHERPESVIRINAE, infecting B-cells in humans. It is thought to be the causative agent of INFECTIOUS MONONUCLEOSIS and is strongly associated with oral hairy leukoplakia (LEUKOPLAKIA, HAIRY;), BURKITT LYMPHOMA; and other malignancies.
Laboratory mice that have been produced from a genetically manipulated EGG or EMBRYO, MAMMALIAN.
An HLA-DR antigen associated with HLA-DRB1 CHAINS that are encoded by DRB1*01 alleles.
Partial proteins formed by partial hydrolysis of complete proteins or generated through PROTEIN ENGINEERING techniques.
Elements of limited time intervals, contributing to particular results or situations.
Subunits of the antigenic determinant that are most easily recognized by the immune system and thus most influence the specificity of the induced antibody.
Vaccines or candidate vaccines designed to prevent or treat cancer. Vaccines are produced using the patient's own whole tumor cells as the source of antigens, or using tumor-specific antigens, often recombinantly produced.
HLA-DR antigen subtypes that have been classified according to their affinity to specific ANTIBODIES. The DNA sequence analyses of HLA-DR ALPHA-CHAINS and HLA-DR BETA-CHAINS has for the most part revealed the specific alleles that are responsible for each serological subtype.
Antigens produced by various strains of HEPATITIS D VIRUS.
The outward appearance of the individual. It is the product of interactions between genes, and between the GENOTYPE and the environment.
Small synthetic peptides that mimic surface antigens of pathogens and are immunogenic, or vaccines manufactured with the aid of recombinant DNA techniques. The latter vaccines may also be whole viruses whose nucleic acids have been modified.
Includes the spectrum of human immunodeficiency virus infections that range from asymptomatic seropositivity, thru AIDS-related complex (ARC), to acquired immunodeficiency syndrome (AIDS).
Glycoproteins with a wide distribution on hematopoietic and non-hematopoietic cells and strongly expressed on macrophages. CD58 mediates cell adhesion by binding to CD2; (ANTIGENS, CD2); and this enhances antigen-specific T-cell activation.
The range or frequency distribution of a measurement in a population (of organisms, organs or things) that has not been selected for the presence of disease or abnormality.
A specific immune response elicited by a specific dose of an immunologically active substance or cell in an organism, tissue, or cell.
A major histocompatibily complex class I-like protein that plays a unique role in the presentation of lipid ANTIGENS to NATURAL KILLER T-CELLS.
Local surface sites on antibodies which react with antigen determinant sites on antigens (EPITOPES.) They are formed from parts of the variable regions of FAB FRAGMENTS.
The insertion of recombinant DNA molecules from prokaryotic and/or eukaryotic sources into a replicating vehicle, such as a plasmid or virus vector, and the introduction of the resultant hybrid molecules into recipient cells without altering the viability of those cells.

Association of major histocompatibility complex determinants with the development of symptomatic and asymptomatic genital herpes simplex virus type 2 infections. (1/593)

The clinical spectrum of herpes simplex virus (HSV) infections, ranging from asymptomatic to frequently distressing outbreaks, suggests that there may be immunologic determinants of disease severity that are associated with human leukocyte antigen (HLA) expression. A controlled, prospective study identified several major histocompatibility complex (MHC) class I and II antigens whose frequencies are associated with HSV-2 infection or with frequent symptomatic genital recurrences. Previous studies were hampered by the inability to serologically identify patients with asymptomatic HSV-2 infection. Clinical evaluation and Western blot assay were used to identify 3 subject cohorts: 1 with no prior HSV infections, 1 with HSV-2 antibodies but no recognized symptoms, and 1 with HSV-2 antibodies and frequent genital recurrences. Statistical comparisons of HLA frequencies among these cohorts showed associations of HLA-B27 and -Cw2 with symptomatic disease. Also, HLA-Cw4 was significantly associated with HSV-2 infection. These associations indicate that immunologic factors linked to the MHC influence the risk of HSV-2 infection and disease expression.  (+info)

Common intra-articular T cell expansions in patients with reactive arthritis: identical beta-chain junctional sequences and cytotoxicity toward HLA-B27. (2/593)

Spondyloarthropathies constitute a group of autoimmune diseases of special interest because of their tight association with the MHC class I molecule HLA-B27 and the bacterial triggering of some clinical forms called reactive arthritis (ReA). One current hypothesis is the presentation by HLA-B27 of a so-called arthritogenic peptide to T cells. To better focus on the relevant T cell populations within the joint, we performed an extensive beta-chain T cell repertoire analysis of synovial fluid compared with PBL in seven patients, four of whom were characterized as having ReA triggered by Yersinia enterocolitica, Chlamydia trachomatis, or Shigella sonnei. Analysis of the size diversity of the beta-chain complementarity-determining region 3 (CDR3) allowed us to evaluate the degree of T cell clonality in the samples. Oligoclonal T cell expansions were frequently observed in the joint. In one patient, CDR3 amino acid sequences of major expansions using two different BV genes were identical. One dominant T cell expansion and several CDR3 amino acid sequences were identical in two different patients. Furthermore, one sequence was identical with a sequence reported independently in a Salmonella-induced ReA patient. Together, these data indicate a surprisingly high degree of conservation in the T cell responses in recent-onset ReA triggered by different micro-organisms. A CD8+ synovial line expressing shared clonotypes was established and reacted toward several B*2705 lymphoblastoid cell lines, therefore supporting a molecular mimicry phenomenon at the T cell level in the disease mechanism.  (+info)

Pathogenesis of reactive arthritis. (3/593)

Reactive arthritis is a member of the spondyloarthropathy. Bacteria which cause reactive arthritis infect the mucosal surfaces. Either the whole bacteria or their fragments are subsequently carried to the joints inside which are induced a TH1 lymphocyte response in which oligoclonal T lymphocytes as well peptide-specific CD8+ T lymphocytes participate. Human lymphocyte antigen (HLA)-B27 is a predisposing gene. Besides being determinants for the CD8+ T lymphocyte response it can also modify the response of other cells to the invasive bacteria. This would lead to alteration of the fate of the bacteria as well as release of arthritis-causing cytokines.  (+info)

Cutting edge: HLA-B27 can form a novel beta 2-microglobulin-free heavy chain homodimer structure. (4/593)

HLA-B27 has a striking association with inflammatory arthritis. We show that free HLA-B27 heavy chains can form a disulfide-bonded homodimer, dependent on residue Cys67 in their extracellular alpha 1 domain. Despite the absence of beta 2-microglobulin, HLA-B27 heavy chain homodimers (termed HC-B27) were stabilized by a known peptide epitope. HC-B27 complexes were recognized by the conformation-specific Ab W6/32, but not the ME1 Ab. Surface labeling and immunoprecipitation demonstrated the presence of similar W6/32-reactive free heavy chains at the surface of HLA-B27-transfected T2 cells. HC-B27 homodimer formation might explain the ability of HLA-B27 to induce spondyloarthropathy in beta 2-microglobulin-deficient mice.  (+info)

Cell-surface expression and alloantigenic function of a human nonclassical class I molecule (HLA-E) in transgenic mice. (5/593)

We have introduced the gene (E*01033) encoding the heavy chain of the human nonclassical MHC class I Ag, HLA-E, into the mouse genome. Two founder mice carry a 21-kb fragment, the others bear an 8-kb fragment. Each of the founder mice was mated to mice of an already established C57BL/10 transgenic line expressing human beta2-microglobulin (beta2m). Cell surface HLA-E was detected on lymph node cells by flow cytometry only in the presence of endogenous human beta2m. However, HLA-E-reactive mouse CTL (H-2-unrestricted) lysed efficiently the target cells originating from HLA-E transgenic mice without human beta2m, showing that the HLA-E protein can be transported to the cell surface in the absence of human beta2m, presumably by association with murine beta2m. Rejection of skin grafts from HLA-E transgenic mice demonstrates that HLA-E behaves as a transplantation Ag in mice. HLA-E transgenic spleen cells are effective in stimulating an allogeneic CTL response in normal and human classical class I (HLA-B27) transgenic mice. Furthermore, results from split-well analysis indicate that the majority of the primary in vivo-induced CTL recognizes HLA-E as an intact molecule (H-2-unrestricted recognition) and not as an HLA-E-derived peptide presented by a mouse MHC molecule, although a small fraction (ranging from 4 to 21%) of the primary in vivo-induced CTL is able to recognize HLA-E in an H-2-restricted manner. Based on these observations, we conclude that HLA-E exhibits alloantigenic properties that are indistinguishable from classical HLA class I molecules when expressed in transgenic mice.  (+info)

Differential induction of colitis and gastritis in HLA-B27 transgenic rats selectively colonized with Bacteroides vulgatus or Escherichia coli. (6/593)

Resident bacteria play an important role in initiating and perpetuating gastrointestinal inflammation. We previously demonstrated that six commensal bacteria including Bacteroides vulgatus caused more aggressive colitis and gastritis in HLA-B27 transgenic rats than did the other five bacteria without B. vulgatus. This study compared the degree of gastrointestinal inflammation in gnotobiotic HLA-B27 transgenic rats monoassociated with either B. vulgatus or Escherichia coli. Gnotobiotic transgenic rats raised in Trexler isolators were selectively colonized with either B. vulgatus or E. coli. Control rats were either germfree or colonized with six common commensal bacteria (Streptococcus faecium, E. coli, Streptococcus avium, Eubacterium contortum, Peptostreptococcus productus, and B. vulgatus [DESEP-B]). After 1 month, all the rats were killed and tissues were prepared for histologic and biochemical evaluation. Colitis induced by B. vulgatus monoassociation was almost equal to that in DESEP-B-colonized rats and was significantly more severe than E. coli-induced colitis, which was absent by histological testing and mild by colonic myeloperoxidase and interleukin-1beta concentration determinations. However, gastritis was detectable only in DESEP-B-associated rats. These studies suggest that not all resident bacteria have equal proinflammatory capabilities, since B. vulgatus alone is more active than E. coli alone in inducing colitis, and that colitis and gastritis result from different luminal bacterial stimuli.  (+info)

Chlamydia pneumoniae as a triggering infection in reactive arthritis. (7/593)

OBJECTIVE: To determine the role of Chlamydia pneumoniae as a triggering infection in reactive arthritis (ReA). METHODS: Sixty patients with acute arthritis were screened for the evidence of triggering infections. In all patients, bacterial stool cultures, culture of Chlamydia trachomatis in urethra/cervix, and/or bacterial serology were studied. Chlamydia pneumoniae antibodies were measured by specific microimmunofluorescence test. RESULTS: Thirty-five of 60 patients fulfilled the diagnostic criteria for ReA. Thirty-one patients had microbial/serological evidence of preceding infection due to Salmonella, Yersinia, Campylobacter or Chlamydia trachomatis, or they had enteritis or urethritis prior to arthritis. Four additional patients had high antibody titre for C. pneumoniae. Three of these four patients had preceding lower respiratory symptoms, and were positive for HLA-B27. The clinical picture of C. pneumoniae-positive ReA patients was similar to that of ReA patients with other definite aetiology. CONCLUSION: Chlamydia pneumoniae is a triggering factor in approximately 10% of patients with acute ReA.  (+info)

Human monocytic U937 cells kill Salmonella in vitro by NO-independent mechanisms. (8/593)

Nitric oxide (NO) has a central role in host defense against intracellular microbes. HLA-B27 has been shown to directly modulate host-microbe interaction in vitro, leading to the impaired elimination of Salmonella in human monocytic U937 cells. Here, we studied whether impaired elimination of Salmonella would result from differences in NO production between HLA-B27- and HLA-A2-transfected U937 cells. Both human monocytic transfectants produced NO equally well and killed Salmonella via NO-independent mechanisms.  (+info)

Several HLA class I alleles have been associated with slow human immunodeficiency virus (HIV) disease progression, supporting the important role HLA class I-restricted cytotoxic T lymphocytes (CTL) play in controlling HIV infection. HLA-B63, the serological marker for the closely related HLA-B*1516 and HLA-B*1517 alleles, shares the epitope binding motif of HLA-B57 and HLA-B58, two alleles that have been associated with slow HIV disease progression. We investigated whether HIV-infected individuals who express HLA-B63 generate CTL responses that are comparable in breadth and specificity to those of HLA-B57/58-positive subjects and whether HLA-B63-positive individuals would also present with lower viral set points than the general population. The data show that HLA-B63-positive individuals indeed mounted responses to previously identified HLA-B57-restricted epitopes as well as towards novel, HLA-B63-restricted CTL targets that, in turn, can be presented by HLA-B57 and HLA-B58. HLA-B63-positive subjects
TY - JOUR. T1 - Qualitative and quantitative differences in peptides bound to HLA-B27 in the presence of mouse versus human tapasin define a role for tapasin as a size-dependent peptide editor. AU - Sesma, Laura. AU - Galocha, Begoña. AU - Vázquez, Miriam. AU - Purcell, Anthony W.. AU - Marcilla, Miguel. AU - McCluskey, James. AU - López De Castro, José A.. PY - 2005/6/15. Y1 - 2005/6/15. N2 - Tapasin (Tpn) is a chaperone of the endoplasmic reticulum involved in peptide loading to MHC class I proteins. The influence of mouse Tpn (mTpn) on the HLA-B*2705-bound peptide repertoire was analyzed to characterize the species specificity of this chaperone. B*2705 was expressed on Tpn-deficient human 721.220 cells cotransfected with human (hTpn) or mTpn. The heterodimer to β2-microglobulin-free H chain ratio on the cell surface was reduced with mTpn, suggesting lower B*2705 stability. The B*2705-bound peptide repertoires loaded with hTpn or mTpn shared 94-97% identity, although significant ...
OBJECTIVE: Association of position 97 (P97) residue polymorphisms in human leucocyte antigen (HLA)-B, including HLA-B*27, with ankylosing spondylitis (AS) has recently been reported. We studied the effect of P97 variations on cell surface expression of the AS-associated HLA-B*27 and HLA-B*51, and the AS-protective HLA-B*7. METHODS: Flow cytometry was used to measure surface expression of HLA-B*27 in C1R/HeLa cells expressing HLA-B*27 (N97) and six mutants at P97 (N97T, N97S, N97V, N97R, N97W and N97D). Transporter associated with antigen processing-deficient T2, tapasin-deficient 220, β2m-deficient HCT15 and endoplasmic reticulum aminopeptidase 1 or β2m-clustered regularly interspaced short palindromic repeats/Cas9-knockout HeLa cells were used to provide evidence for specific protein interactions. Surface expression of HLA-B*7/HLA-B*51 P97 mutants was also studied. RESULTS: Mutation of HLA-B*27 P97 to the AS risk residue threonine increased cell surface free heavy chain (FHC) expression. Protective
HLA-B59 (B59) is an HLA-B serotype. The serotype identifies the more common HLA-B*## gene products. B59 is a hybrid between B*55 and B*51. B59 is more common in Japan, Korea, N. China and Mongolia. (For terminology help see: HLA-serotype tutorial) Marsh SG, Albert ED, Bodmer WF, et al. (2005). Nomenclature for factors of the HLA system, 2004. Tissue Antigens. 65 (4): 301-69. doi:10.1111/j.1399-0039.2005.00379.x. PMID 15787720. Hildebrand WH, Domena JD, Parham P (1993). Primary structure shows HLA-B59 to be a hybrid of HLA-B55 and HLA-B51, and not a subtype of HLA-B8. Tissue Antigens. 41 (4): 190-5. doi:10.1111/j.1399-0039.1993.tb02001.x. PMID 8362411. derived from IMGT/HLA Middleton D, Menchaca L, Rood H, Komerofsky R (2003). New allele frequency database: http://www.allelefrequencies.net. Tissue Antigens. 61 (5): 403-7. doi:10.1034/j.1399-0039.2003.00062.x. PMID 12753660. External link in ,title= (help ...
Immune control of human immunodeficiency virus type 1 (HIV) infection is typically associated with effective Gag-specific CD8 T-cell responses. We here focus on HLA-B*14, which protects against HIV disease progression, but the immunodominant HLA-B*14-restricted anti-HIV response is Env specific (ERYLKDQQL, HLA-B*14-EL9). A subdominant HLA-B*14-restricted response targets Gag (DRYFKTLRA, HLA-B*14-DA9). Using HLA-B*14/peptide-saporin-conjugated tetramers, we show that HLA-B*14-EL9 is substantially more potent at inhibiting viral replication than HLA-B*14-DA9. HLA-B*14-EL9 also has significantly higher functional avidity ( < 0.0001) and drives stronger selection pressure on the virus than HLA-B*14-DA9. However, these differences were HLA-B*14 subtype specific, applying only to HLA-B*14:02 and not to HLA-B*14:01. Furthermore, the HLA-B*14-associated protection against HIV disease progression is significantly greater for HLA-B*14:02 than for HLA-B*14:01, consistent with the superior antiviral ...
OBJECTIVE: To fully define cytotoxic T-lymphocyte (CTL) escape variants of an HLA-B*51-restricted integrase epitope in early HIV-1 infection. DESIGN: Ninety-four longitudinally sampled acute/early HIV-1 subtype B-infected participants were assessed to determine HLA-B*51-restricted LPPVVAKEI (LI9) escape variants. METHODS: LI9 was sequenced at baseline and subsequent time points. Interferon-γ (IFNγ) ELISpot assays were performed using serial log dilutions of variant LI9 peptides to determine the cellular response and functional avidity. RESULTS: There is a significant association between HLA-B*51 expression and an evolving LI9 sequence from baseline to year 1 (P | 0.0001). We detected that the V32I and P30X polymorphisms emerged within HLA-B*51 participants over time. Reversion of the P30S polymorphism was observed by year 1 in one HLA-B*51 participant. LPPIIAKEI and LPSIVAKEI had significantly lower functional avidity compared with LPPVVAKEI and so may be less well recognized by LI9-specific CTLs; a
HLA-B53 (B53) is an HLA-B serotype. The serotype identifies the more common HLA-B*53 gene products.[1] The B53 sequence is identical to B35 but short sequence specifies a Bw4 rather than a Bw6 motif (as found in B35), indicating B53 is a recent product of gene conversion.[2] This suggests an origin for HLA-B53 involving a gene conversion of HLA-B35 by an allele containing this Bw4 sequence. ((For terminology help see: HLA-serotype tutorial) ...
B*2701 differs from B*2705-by three amino acid changes: D--|Y74, D--|N77, L--|A81, and from B*2702 only by two: D--|Y74 and T--|I80. Tyr74 is located in the C/F cavity of the peptide-binding site, and is unique to B*2701 among HLA-B27 subtypes. Binding of natural B*2705 and B*2702 lig …
Polymorphism in the HLA region of a chromosome is the major source of host genetic variability in HIV-1 outcome, but there is limited understanding of the mechanisms underlying the beneficial effect of protective class I alleles such as HLA-B57, -B27, and -B51. Taking advantage of a unique cohort infected with clade B HIV-1 through contaminated blood, in which many variables such as the length of infection, the infecting viral strain, and host genetic background are controlled, we performed a comprehensive study to understand HLA-B51-associated HIV-1 control. We focused on the T cell responses against three dominant HLA-B51-restricted epitopes: Gag327-345(NI9) NANPDCKTI, Pol743-751(LI9) LPPVVAKEI, and Pol283-289(TI8) TAFTIPSI. Mutations in all three dominant epitopes were significantly associated with HLA-B51 in the cohort. A clear hierarchy in selection of epitope mutations was observed through epitope sequencing. L743I in position 1 of epitope LI9 was seen in most B51+ individuals, followed ...
This study is a cross-sectional observational study to evaluate the prevalence of HLA-B*5701 in the European area and in major European ethnotypes.. Any HIV-1 infected patient will be eligible for this study including treatment naïve and experienced patients, as well as patients previously tested for HLA-B*5701. Patients will be approached during a standard clinic visit, and will be consented prior to any study specific procedure. They will be asked to provide a tissue sample (cheek cells and blood sample) which will be used to assess HLA-B*5701 status by local and central laboratories.. In selected sites patients may be asked to provide an additional blood sample. This sample will be used to develop and validate different methodologies for assessing HLA-B*5701 status. ...
A remarkable feature of the association of ERAP2 with AS is that, unlike ERAP1, there is no epistasis with HLA-B*27 because ERAP2 is associated with AS among HLA-B*27 positive and negative individuals (16). HLA-B*40:02 is one of a small group of non-B*27 HLA-B allotypes conferring risk to AS (14) independently of HLA-B*27. Like the latter, HLA-B*40:02 has a high percentage of basic P1 residues, which are known targets of ERAP2. However, due to the strong preference for acidic P2 residues the B*40:02 peptidome is quite distinct from that of HLA-B*27, which mainly binds peptides with R at this position (22⇓-24).. The effects of ERAP2 on MHC-I bound peptidomes is insufficiently characterized, although the role of this enzyme on HLA-B*27:05 (17, 18), HLA-A*29:02 (19), and HLA-B*51:01 (20) has been reported. These studies showed that the major effects of ERAP2 are quantitative, influencing peptide amounts, and allotype dependent. For instance, in B*27:05 the presence of ERAP2 lead to a moderate ...
In studies of antigenic peptide presentation, we have found a healthy volunteer whose lymphoblastoid cells were unable to present three different virus-derived epitopes to cytotoxic T lymphocytes (CTL) despite expressing the correct restricting HLA-B27 molecules on the cell surface. B cell lines were established from other members of the donors family, including individuals suffering from ankylosing spondylitis and related diseases, and were tested for their ability to function as target cells in the same assay. None of the eight B cell lines that expressed HLA-B27 presented a known peptide epitope to CTL. However, cells from a family member that expressed HLA-B8 could present an epitope peptide restricted by that molecule. The B27 molecule in this family proved to be the B2702 subtype on isoelectric focusing gels, appearing in exactly the same position as B2702 from other cell lines that did present the peptide. To exclude mutations resulting in noncharged amino acid substitutions, cDNA coding ...
The genetic polymorphism that has the greatest impact on immune control of human immunodeficiency virus (HIV) infection is expression of HLA-B*57. Understanding of the mechanism for this strong effect remains incomplete. HLA-B*57 alleles and the closely related HLA-B*5801 are often grouped together
This is a study to determine the prevalence of a genetic marker, HLA-B5701, in the UK population. HLA-B*5701 has been strongly associated with the risk of an allergic reaction to a HIV medicine, Abacavir. The allergic reaction is known as a hypersensitivity reaction.. The study is a prospective study inviting HIV-1 positive patients over the age of 18 years to participate. Each participant will complete one study visit. They will be asked for details of their background including their age, sex, ethnicity, country of origin and parental country of origin.. They will be asked to give two samples to test for the presence of the genetic marker HLA-B*5701. The two samples are:. ...
The polymorphism of HLA-B27 alleles is located in the peptide-anchoring motif. In recent years, fundamental insights have been made into the molecular aspects of HLA-B27-restricted presentation. Subtle differences in peptide binding fine specificity are especially interesting for closely related HLA …
sub type, and it has the same probabilities.. So, even if there is such a big uncertainty in the results, it does give a very crude indication, even for the small reference data set. And, it did match up with the result of the blood test, which was fun, but could just as well have been pure luck.. Bottom line: Its pretty awesome that ordinary people can do stuff like this. I can easily imagine a professional service built around imputation: Patient gets genotyped, its stored on a secure server. The doctor can then, based on a permission scheme, run imputation for things like HLA-B27 as one of many tools when diagnosing. A blood test will always be more accurate than imputation, but is time consuming and costs money. If a doctor could quickly see that the probability of HLA-B27 is low, and depending on the context, it may not be necessary to order a blood test at all.. ...
HLA-B8 molecule functions as a restriction element for M3-W1-B9 CD8+ T cells. (A) T cell recognition of peptide-pulsed HLA-B8-expressing cell lines. (B) Ident
Specific lysis of HLA-B8-matched EBV-transformed LCLs by M3-W1-B9 CD8+ T cells. (A) Recognition of HLA-B8-matched LCLs by M3-W1-B9 CD8+ T cells. LCLs were c
easYmer HLA-B*46:01 MHC Tetramers Kit can be used to generate monomers with your choice of peptide and to analyze T-cells by flow cytometry.
Inically suspected HSR, HLA-B*5701 features a sensitivity of 44 in White and 14 in Black sufferers. ?The specificity in White and Black control subjects was 96
Principal Investigator:JOKO Satoru,沼賀 二郎, Project Period (FY):1995 - 1996, Research Category:Grant-in-Aid for Scientific Research (C), Section:一般, Research Field:Ophthalmology
HLA-B27 is a human leukocyte antigen that helps the body differentiate its own cells from foreign substances. A test indicating that HLA-B27 is present means that the patient might have a risk of...
reference: Structural basis for T cell alloreactivity among three HLA-B14 and HLA-B27 antigens., Kumar P, Vahedi-Faridi A, Saenger W, Merino E, Lopez de Castro JA, Uchanska-Ziegler B, Ziegler A, J Biol Chem. 2009 Jul 18. PMID: 19617632 ...
The HLA-B*52:01 and -C*12:02-restricted CTLs strongly suppress HIV-1 replication resulting in slower disease progression. In contrast, the HLA-B*52:01-C*12:02 haplotypes are suscep ...
Purpose: : Non-granulomatous anterior uveitis, sometimes with hypopyon, is commonly seen in the patients with HLA-B27 associated acute anterior uveitis (AAU) or with Behcets disease (BD). Although in BD, the inflammation distinguishingly expands into the posterior segment, the clinical features between these two diseases are still unclear. The purpose of this study is to clarify the different features of anterior uveitis between these two diseases. Methods: : We conducted a prospective study and compared 20 eyes of 16 patients with HLA-B27 associated AAU ( HLA-B27 Group) and 79 eyes of 43 patients with BD ( BD group) who satisfied the criteria by the International Study Group for Behcets disease. All patients had frequent recurrences of acute inflammation. The patients were followed up from April 2003 to October 2005. The total number of relapses was 44 times in the HLA-B27 Group and 174 times in the BD Group. For treatment of anterior uveitis, every patient in this study was treated by ...
PURPOSE: To describe the evolution of retinal thickness in eyes affected with acute anterior uveitis (AAU) in the course of follow-up and to assess its correlation with severity of inflammatory activity in the anterior chamber.. METHODS: 72 eyes (affected and fellow eyes) of 36 patients presenting with HLA-B27-related acute anterior uveitis were included in a prospective, institutional, cohort study. Patients were followed daily until beginning of resolution of inflammatory activity and weekly thereafter. Optical coherence tomography and laser flare photometry were performed at each visit. Treatment consisted of topical corticosteroids. Main outcome measures were retinal thickness of affected eyes, difference in retinal thickness between affected and fellow eyes and their evolution in time, association between maximal retinal thickness, and initial laser flare photometry.. RESULTS: Difference in retinal thickness between affected and fellow eyes became significant on average 7 days from baseline ...
Reactive arthritis, also known as Reiters syndrome, is a type of arthritis that occurs as a reaction to an infection somewhere in the body. Most infections that cause the disease originate in the genitourinary tract (the bladder, urethra, penis, or vagina) and are spread through sexual intercourse, a form of the disease called genitourinary Reiters syndrome, or urogenital Reiters syndrome. Other infections that can cause reactive arthritis include gastrointestinal infections due to eating contaminated food or handling contaminated substances, a form of the disease called gastrointestinal Reiters syndrome, or enteric Reiters syndrome.. ...
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OBJECTIVE: HLA-B27 is capable of forming in vitro a heavy-chain homodimer structure lacking beta(2)-microglobulin. We undertook this study to ascertain if patients with spondylarthritis express beta(2)-microglobulin-free HLA-B27 heavy chains in the form of homodimers and receptors for HLA-B27 homodimers. METHODS: Expression of HLA-B27 heavy chains by mononuclear cells was analyzed by fluorescence-activated cell sorter staining, Western blotting with the monoclonal antibody HC-10, and 2-dimensional isoelectric focusing. Fluorescence-labeled tetrameric complexes of HLA-B27 heavy-chain homodimers were constructed in which each dimer comprised one His-tagged heavy chain and one biotinylated heavy chain, and were used to stain patient and control mononuclear cells and transfected cell lines. RESULTS: Patients with spondylarthritis expressed cell-surface HLA-B27 homodimers. Populations of synovial and peripheral blood monocytes, and B and T lymphocytes from patients with spondylarthritis, and controls carried
The first human leukocyte antigen (HLA) haplotype association with inflammatory disease was discovered in 1972, correlating HLA-B27 with ankylosing spondylitis. This remains one of the strongest known associations of disease with HLA-B27.
Abstract: : Purpose: To assess the systemic manifestations associated with HLA-B27 uveitis. To analyze the characteristics of ocular inflammation. Methods: Monocentric, prospective study of 175 patients seen between January 1996 and March 2001 with acute anterior uveitis and HLA-B27 allele. Patients were systematically referred for a rheumatologic evaluation and diagnosis were based on criteria from the European Spondylarthropathy Study Group. Results: There were 99 males and 76 females. The mean age was 40.4 ± 13.3 years at the time of the study and 34.5 ± 13.9 years at the time of the first episode of uveitis. The average time between the first episode of uveitis and the last visit during the study period was 7.5 ± 7.2 years (median : 5.1). The mean number of episodes of uveitis since the onset of the disease was 4.9 ± 7.9 (median : 3). The mean frequency of recurrences was 0.8 ± 0.6 per year. Posterior segment involvement was observed in 45 (25.7%) patients. Final visual acuity was ...
The association between HLA-B 2705 and the immune control of human immunodeficiency virus type 1 (HIV-1) has previously been linked to the targeting of the HLA-B 2705-restricted Gag epitope KRWIILGLNK (KK10) by CD8(+) T cells. In order to better define the mechanisms of the HLA-B 2705 immune control of HIV, we first characterized the CD8(+) T-cell responses of nine highly active antiretroviral therapy (HAART)-naïve B 2705-positive subjects. Unexpectedly, we observed a strong response to an HLA-B 2705-restricted Pol epitope, KRKGGIGGY (KY9), in 8/9 subjects. The magnitude of the KY9 response was only marginally lower than that of the KK10-specific response (median, 695 versus 867 spot-forming cells [SFC]/million peripheral blood mononuclear cells [PBMCs]; not significant [NS]), and viral escape mutants were observed in both KY9 and KK10, resulting from selection pressure driven by the respective CD8(+) T-cell response. By comparing inhibitions of viral replication by CD8(+) T cells specific for the Gag
In previous studies of antigen presentation through HLA-B27, we identified a healthy person whose lymphoblastoid cells do not present three B27-restricted viral epitopes to specific cytotoxic T lymphocytes (CTL), despite adequate cell surface expression of HLA-B2702 of normal sequence. Similar findings were observed in all members of his family sharing the HLA-A3-B2702 haplotype. The original donor, NW, carries HLA-B8 on his other class I haplotype, which his daughter, HW, has inherited. We now report a failure to present an HLA-B8-restricted epitope from influenza nucleoprotein following viral infection of NW cells, although exogenous added peptide is still presented normally. However, cells from HW, which do not carry the A3-B2702 haplotype, present the expected epitope after viral infection. Another B8-restricted epitope, from human immunodeficiency virus-gag, is presented equally well by both cell lines when infected with gag-vaccinia. This antigen processing phenotype does not correlate with any of
HLA-B57 (B57) is an HLA-B serotype. B57 is a split antigen from the B17 broad antigen, the sister serotype B58. The serotype identifies the more common HLA-B*58 gene products. (For terminology help see: HLA-serotype tutorial) Like B58, B57 is involved in drug-induced inflammatory skin disorders. HLA-B*5701 is associated with drug-induced inflammatory disease of the skin. Individuals with B57 are more sensitive to the drug abacavir. Abacavir is an antiretroviral drug used in treatment of HIV, however in sensitive individuals fever, skin rash, fatigue, gastrointestinal symptoms such as nausea, vomiting, diarrhea or abdominal pain and respiratory symptoms such as pharyngitis, dyspnea, or cough can develop. FDA has advised that people from at-risk ethnic groups, (see table on the left) be screened prior to drug-therapy. [Note: phenotype frequencies are roughly double allele frequencies -tabled values- when allele frequency is less than 30%] Ways JP, Coppin HL, Parham P (1985). The complete primary ...
Objective: To investigate the relationship between intestinal inflammation and the central and peripheral innate immune system, in the pathogenesis of HLA-B27 associated spondyloarthritis. Methods: The myeloid compartment of the bone marrow and blood of HLA-B27 transgenic (B27), control HLA-B7 transgenic (B7), and non-transgenic rats were evaluated by flow cytometry. Plasma from rats were assessed by ELISA for CCL2 and IL-1α levels. Rats were treated for 4 weeks with antibiotics and the blood and bone marrow myeloid compartments were evaluated by flow cytometry. The osteoclastogenic potential of bone marrow cells from antibiotic treated rats, in the presence or absence of TNFα, was evaluated in vitro. Results: B27 rats have substantially higher numbers of circulating Lin-CD172a+CD43l° monocytes than control animals, which significantly correlates with higher levels of plasma CCL2. Antibiotic treatment of B27 rats markedly reduced ileitis, plasma CCL2 and IL-1α levels, and the number of bone ...
The spondyloarthropathies represent highly enigmatic conditions and although their clinical features, anatomical distribution of disease and genetic predisposing factors have been known for some time, a unified concept of the basic pathobiology underlying these illnesses has remained undefined. Recently progress has been made because numerous independent studies have converged upon IL-23 as a central cytokine in spondyloarthropathy and the mechanism and sites of action of this cytokine have now become much clearer. These findings enable the rational design of therapeutic strategies which it is hoped will profoundly modify the progression of these diseases. We will review the anatomical sites affected and the evidence for the importance of IL-23 in these conditions, before drawing these lines of investigation together to propose a model for the unified understanding of spondyloarthropathy.
Genetic polymorphisms within the MHC encoding region have the strongest impact on HIV disease progression of any in the human genome and provide important clues to the mechanisms of HIV immune control. Few analyses have been undertaken of HLA alleles associated with rapid disease progression. HLA-B*07:02 is an HLA class I molecule that is prevalent in most populations worldwide and that has previously been consistently linked to accelerated disease progression in B-clade infection. This study investigates the observation that HLA-B*07:02 is not associated with a high viral setpoint in C-clade infection. We examine the hypothesis that this clade-specific difference in association with disease outcome may be related to distinct targeting of CD8(+) T cell epitopes. We observed that C-clade-infected individuals with HLA-B*07:02 target a broader range of Gag epitopes, and to higher magnitudes, than do individuals infected with B-clade infection. In particular, a novel p17-Gag (Gag22-30, RPGGKKHYM) epitope is
Supplementary MaterialsSupplementary Materials: Number S1: Sequences of primers utilized for PCR. peptides, KRGILTLKY and SRYWAIRTR, in to the ER with a tat-derived peptide (GRKKRRQRRR)-His6-ubiquitin (THU) automobile. Duloxetine small molecule kinase inhibitor Both peptides derive from the individual nucleoprotein and actin of influenza trojan, respectively. Our outcomes showed that targeted delivery of both HLA-B?27-binding peptides in to the ER may promote the HLA-B?27 folding, reduce the degrees of (B27-HC)2, and suppress the activation from the IL-23/IL-17 axis in response to lipopolysaccharide. Our results can offer a new healing technique in AS. 1. Launch Ankylosing spondylitis (AS) can be an inflammatory disease thats seen as a inflammatory back discomfort and asymmetric peripheral oligoarthritis [1C4]. The introduction of AS is associated with the expression of individual leukocyte antigen-B strongly?27 (HLA-B?27) [5, 6]. A lot more than 90% of AS sufferers exhibit HLA-B?27. HLA-B?27 ...
Merck & Co., Inc., Kenilworth, NJ, USA (known as MSD outside of the US and Canada) is a global healthcare leader working to help the world be well. From developing new therapies that treat and prevent disease to helping people in need, we are committed to improving health and well-being around the world. The Veterinary Manual was first published in 1955 as a service to the community. The legacy of this great resource continues in the online and mobile app versions today. ...
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Mear JP, Schreiber KL, Munz C, Zhu X, Stevanovic S, Rammensee HG, Rowland-Jones SL, Colbert RA. Misfolding of HLA-B27 as a result of its B pocket suggests a novel mechanism for its role in susceptibility to spondyloarthropathies. J Immunol 1999; 163:6665-70 ...
Results. We found 10.1% of UA (38/375) versus 7.2% (403/5584) of controls were HLA-B27-positive (OR 1.5, 95% CI 1.0-2.1; p = 0.037). HLA-B27-positive patients with UA had more SpA features than HLA-B27-negative patients (mean 1.6, SD 1.0, and 0.9 SD 0.6; p , 0.001), but patients with SpA had significantly more SpA features (mean 4.5, SD 1.5; p , 0.001). Family history and preceding infection were features more common in HLA-B27-positive than in HLA-B27-negative UA (15.8% vs 1.3%, p = 0.04 and 15.8% vs 2.6%, p = 0.04). After HLA-B27 testing, 21 additional patients (5.6%) with UA could potentially have been classified with pSpA according to the ASAS criteria. ...
Mouse monoclonal antibody raised against HLA-A/HLA-B/HLA-C. Purified human HLA-A/HLA-B/HLA-C complex. (MAB6383) - Products - Abnova
Research has shown that people with a specific gene known as HLA-B27 have a significantly increased chance of developing reactive arthritis, as well as related conditions such as ankylosing spondylitis (a type of arthritis that affects the spine). In the UK, its estimated that around 1 in every 10 people have the HLA-B27 gene. Around 3 out of every 4 cases of reactive arthritis develop in people with the gene. People with the HLA-B27 gene also tend to have more severe and longer-lasting symptoms, with a greater risk of their symptoms recurring.. Exactly how the gene contributes to the development of reactive arthritis is unclear.. ...
Why low starch? The whole situation relating to HLA-B27, klebsiella and AS is horribly complex, but at the core is the enzyme pullulonase, which provides the peptide sausage which fits the HLA-B27 bun. Ive been trying to find exactly which sugar triplet pullulonase degrades and its not clear from Wiki. What is clear is that the triplet is present in starch and the enzyme pullulonase is produced whenever klebsiella meets starch. The enzyme is surface mounted on the bacterium and is in an ideal position to be seen by the immune system. If an HLA-B27 carrying white blood cell kills a klebsiella microbe it will easily produce the short peptide from pullulonase which fits the HLA-B27 molecule on its surface. That signals to both the innate and the adaptive immune systems to become active and (a) attack and (b) make antibodies against the peptide fragment. Its a bit unfortunate that the antibodies made against pullulanase, presented by HLA-B27, also fit the collagen around your spine, knees and ...
Robinson PC., Costello M-E., Leo P., Bradbury LA., Hollis K., Cortes A., Lee S., Joo KB., Shim S-C., Weisman M., Ward M., Zhou X., Garchon H-J., Chiocchia G., Nossent J., Lie BA., Førre Ø., Tuomilehto J., Laiho K., Jiang L., Liu Y., Wu X., Elewaut D., Burgos-Vargas R., Gensler LS., Stebbings S., Haroon N., Mulero J., Fernandez-Sueiro JL., Gonzalez-Gay MA., Lopez-Larrea C., Bowness P., Gafney K., Gaston JSH., Gladman DD., Rahman P., Maksymowych WP., Xu H., van der Horst-Bruinsma IE., Chou C-T., Valle-Oñate R., Romero-Sánchez MC., Hansen IM., Pimentel-Santos FM., Inman RD., Martin J., Breban M., Evans D., Reveille JD., Kim T-H., Wordsworth BP., Brown MA ...
I am a 40 year old male and have been suffering with rhuematoid arthtritis now for about 18 years, my specialist says it is more like spondyloarthropathy than rhuematoid. I had an accident back in 1991...
Objectives HLA-B*5701 is a major histocompatibility complex class I allele associated with an immunologically-mediated hypersensitivity reaction to abacavir.
Spondyloarthropathy answers are found in the 5-Minute Pediatric Consult powered by Unbound Medicine. Available for iPhone, iPad, Android, and Web.
Altered Regulation of ELAVL1-HuR in HLA-B27-Expressing U937 Monocytic Cells. . Biblioteca virtual para leer y descargar libros, documentos, trabajos y tesis universitarias en PDF. Material universiario, documentación y tareas realizadas por universitarios en nuestra biblioteca. Para descargar gratis y para leer online.
The researchers were surprised to discover that this enhanced mineralization was not regulated by the transcription factor RUNX2, which is usually associated with this process, but was instead regulated by TNAP via the transcription factor RARβ. Further in vitro investigations revealed that unfolded HLA-B27 causes the upregulation of TNAP in AS-derived MSCs via the IRE1α-sXBP1 pathway of the unfolded protein response. Crucially, serum concentrations of bone-specific TNAP were increased in patients with AS compared with healthy individuals from two different cohorts and correlated with disease progression. ...
on JULY 21, 2016 by CHRIS KRESSER 3 comments Do you have an autoimmune disease? Do starchy foods tend to make your symptoms worse? Mounting evidence suggests that individuals who have autoimmunity related to a particular set of genes called HLA-B27 may benefit from reducing their starch intake. Read on to learn why this…
Time to Seroconversion in HIV-Exposed Subjects Carrying Protective versus Non Protective KIR3DS1-L1 and HLA-B Genotypes. . Biblioteca virtual para leer y descargar libros, documentos, trabajos y tesis universitarias en PDF. Material universiario, documentación y tareas realizadas por universitarios en nuestra biblioteca. Para descargar gratis y para leer online.
Guidelines in North America and Europe recommend routine screening for HLA-B*5701 prior to initiation of abacavir therapy.{ref9}{ref10}{ref11}{ref8} However, its necessity in low-risk populations... more
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TY - JOUR. T1 - The occurrence of HLA‐B46 in two Caucasoid families. AU - Hart, J. M.. AU - Zemmour, J.. AU - Schmeckpeper, B. J.. AU - Parham, P.. AU - Wood, W. W.. AU - Hopkins, K. A.. AU - Leffell, M. S.. AU - Bias, W. B.. PY - 1993/1. Y1 - 1993/1. KW - DNA sequence of Caucasoid HLA‐B46. KW - HLA distribution in populations. KW - HLA‐B46 in non‐orientals. KW - gene flow of HLA antigens. UR - http://www.scopus.com/inward/record.url?scp=0027447465&partnerID=8YFLogxK. UR - http://www.scopus.com/inward/citedby.url?scp=0027447465&partnerID=8YFLogxK. U2 - 10.1111/j.1399-0039.1993.tb01977.x. DO - 10.1111/j.1399-0039.1993.tb01977.x. M3 - Article. C2 - 8456442. AN - SCOPUS:0027447465. VL - 41. SP - 47. EP - 50. JO - HLA. JF - HLA. SN - 2059-2302. IS - 1. ER - ...
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prefix fhir: ,http://hl7.org/fhir/, . @prefix loinc: ,http://loinc.org/rdf#, . @prefix owl: ,http://www.w3.org/2002/07/owl#, . @prefix rdfs: ,http://www.w3.org/2000/01/rdf-schema#, . @prefix xsd: ,http://www.w3.org/2001/XMLSchema#, . # - resource ------------------------------------------------------------------- a fhir:Observation; fhir:nodeRole fhir:treeRoot; fhir:Resource.id [ fhir:value HLA-B-AlleleExample2]; fhir:Resource.meta [ fhir:Meta.profile [ fhir:value http://fhir.nmdp.org/ig/hla-reporting/StructureDefinition/hla-allele; fhir:index 0; fhir:link ,http://fhir.nmdp.org/ig/hla-reporting/StructureDefinition/hla-allele, ] ]; fhir:DomainResource.text [ fhir:Narrative.status [ fhir:value generated ]; fhir:Narrative.div ,div xmlns=\http://www.w3.org/1999/xhtml\,,p,,b,Generated Narrative with Details,/b,,/p,,p,,b,id,/b,: HLA-B-AlleleExample2,/p,,p,,b,meta,/b,: ,/p,,p,,b,status,/b,: final,/p,,p,,b,category,/b,: Laboratory ,span style=\background: LightGoldenRodYellow\,(Details : ...
This study is assessing the pharmacodynamics of infliximab in patients with spondylarthritis. The coprimary endpoints are the expression of surface markers and
The biological assembly is a heterotrimeric complex consisting of one HLA-B*2709 (heavy) chain, one beta-2-microglobulin (light) chain and one decameric model peptide s10R ...
May 2, 2017 ... Sabrina Gmuca, Rui Xiao, [. ... HLA-B27 positivity in this group of children also seems to vary by geographic location. Cohorts from India and ... ...
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運動是使得腎上腺素分泌的一種生理刺激。第一次證明是使用貓的去神經支配的瞳孔來測定,後來使用尿液樣品的生物測定法確認。1950年公佈了生物化學方法用於測量血漿中兒茶酚胺。雖然使用熒光測定法測量總兒茶酚胺濃度已經發表了許多寶貴的作法,但該方法用以準確地測定血漿中非常少量的腎上腺素有過於非特異性和不敏感的問題。提取方法和酶同位素衍生物放射性酶測定(REA)的發展將分析轉化為靈敏度1皮克的腎上腺素。早期REA血漿測定表明,腎上腺素和總兒茶酚胺在運動後期上升,主要是在無氧代謝開始時。 ...
Presence of the HLA-B27 antigen; (3) Onset of arthritis in a male over 6 years of age; (4) Acute (symptomatic) anterior uveitis ... HLA-B27, Rheumatoid factor and Anti-citrullinated protein antibody. These serological markers may be negative in children with ... The Anti-nuclear antigen (ANA) is positive in up to 80% of patients with oligoarthritis and is associated with a higher risk of ...
That is, persons who have the human leukocyte antigen B27 (HLA-B27) are most susceptible. Most often, the symptoms of reactive ... C jejuni antigens that cross-react with one or more neural structures may be responsible for triggering the Guillain-Barré ... C. jejuni secretes Campylobacter invasive antigens (Cia) which facilitates the motility. The bacteria produces also cytolethal ...
There is an association with the HLA-B27 histocompatibility antigen, which is present in 65% of patients with erythema nodosum ... Erythema nodosum is probably a delayed hypersensitivity reaction to a variety of antigens. Although circulating immune ...
More than 85% of those affected in the UK have a specific human leukocyte antigen known as the HLA-B27 antigen. The underlying ... Those with the HLA-B27 variant are at a higher risk than the general population of developing the disorder. HLA-B27, ... 1-2% of individuals with the HLA-B27 genotype develop the disease. Approximately 85% of people with AS express the HLA-B27 ... This is thought to be due to the association that both AS and uveitis have with the inheritance of the HLA-B27 antigen. ...
1992). "Asymmetric selection of T cell antigen receptor alpha- and beta-chains in HLA-B27 alloreactivity". J. Immunol. 148 (11 ...
The most common antigens include HLA-B27, HLA-A29 (in birdshot chorioretinopathy) and HLA-B51 (in Behçet disease).[citation ... HLA-B27 AAU can be associated with ocular inflammation alone or in association with systemic disease. HLA-B27 AAU has ... However, anterior uveitis is often one of the syndromes associated with HLA-B27. Presence of this type of HLA allele has a ... Wakefield D, Chang JH, Amjadi S, Maconochie Z, Abu El-Asrar A, McCluskey P (April 2011). "What is new HLA-B27 acute anterior ...
... hla-b27 antigen MeSH D23.050.301.500.450.380.388 - hla-b35 antigen MeSH D23.050.301.500.450.390 - hla-c antigens MeSH D23.050. ... hla-b27 antigen MeSH D23.050.705.552.400.380.388 - hla-b35 antigen MeSH D23.050.705.552.400.390 - hla-c antigens MeSH D23.050. ... hla-b27 antigen MeSH D23.050.705.552.450.380.388 - hla-b35 antigen MeSH D23.050.705.552.450.390 - hla-c antigens MeSH D23.050. ... hla-a antigens MeSH D23.050.301.500.450.370.372 - hla-a1 antigen MeSH D23.050.301.500.450.370.374 - hla-a2 antigen MeSH D23.050 ...
Ahn JK, Park YG (October 2007). "Human leukocyte antigen B27 and B51 double-positive Behçet uveitis". Arch. Ophthalmol. 125 (10 ... derived from IMGT/HLA *^ a b c d e f g h i j k l m n o p q r s t u v w x y z aa ab ac ad ae af ag ah ai aj ak al am an ao ap aq ... EBI-HLA B*5101 1e28​, 1e27​ HLA-B51 (B51) is an HLA-B serotype. The serotype identifies the more common HLA-B*51 gene products. ... B51 is a split antigen of the broad antigen B5, and is a sister serotype of B52.[2] There are a large number of alleles within ...
Human leukocyte antigen (HLA) B27 (subtypes B*2701-2759) is a class I surface antigen encoded by the B locus in the major ... The prevalence of HLA-B27 varies markedly in the general population. For example, about 8% of Caucasians, 4% of North Africans ... HLA-B27 is strongly associated with ankylosing spondylitis (AS), and other associated inflammatory diseases referred to as " ... In northern Scandinavia (Lapland), 24% of people are HLA-B27 positive, while 1.8% have associated ankylosing spondylitis. A ...
... may be caused by a bacterial antigen; the occurrence of this syndrome is strongly linked to HLA-B27 genotype, but the ...
"HLA antigens in ankylosing spondylitis associated with Crohn's disease. Increased frequency of the HLA phenotype B27,B44". J. ... The serotype identifies the B*44 gene-allele protein products of HLA-B. B44 is a split antigen of the broad antigen B12, and is ... derived from IMGT/HLA Johnston DT, Mehaffey G, Thomas J, et al. (June 2006). "Increased frequency of HLA-B44 in recurrent ... HLA-B44 increases recurrent sinopulmonary infections. Protective effects: HLA-B44 appears to be protective against autoimmune ...
... (B39) is an HLA-B serotype. The serotype identifies the more common HLA-B*39 gene products. B39 is a split antigen of ... Sobao Y, Tsuchiya N, Takiguchi M, Tokunaga K (January 1999). "Overlapping peptide-binding specificities of HLA-B27 and B39: ... Genetics and HLA antigens". Baillière's Clinical Rheumatology. 8 (2): 263-76. doi:10.1016/S0950-3579(94)80018-9. PMID 8076387. ... Gladman DD, Farewell VT (June 1995). "The role of HLA antigens as indicators of disease progression in psoriatic arthritis. ...
HLA-B27 is an example. It is unclear how exactly having the HLA-B27 tissue type increases the risk of ankylosing spondylitis ... The human MHC is also called the HLA (human leukocyte antigen) complex (often just the HLA). Similarly, there is SLA (Swine ... The most studied HLA genes are the nine classical MHC genes: HLA-A, HLA-B, HLA-C, HLA-DPA1, HLA-DPB1, HLA-DQA1, HLA-DQB1, HLA- ... The most diverse loci, namely HLA-A, HLA-B, and HLA-C, have roughly 6000, 7200, and 5800 known alleles, respectively. Many HLA ...
but also some related work in rheumatoid arthritis and in ankylosing spondylitis and its association with HLA-B27. Russell's ... "Cell Surface Prostate Cancer Antigen for Diagnosis". Retrieved 22 May 2019. CS1 maint: discouraged parameter (link) "Minomic ... "Is a Klebsiella plasmid involved in the aetiology of ankylosing spondylitis in HLA-B27-positive individuals?." Molecular ... "Is a Klebsiella plasmid involved in the aetiology of ankylosing spondylitis in HLA-b27-positive individuals?". Molecular ...
Mutations in the ARTS-1 have been linked to an increased risk of ankylosing spondylitis but only in HLA-B27 positive patients. ... affecting antigen presentation to cytotoxic T lymphocytes Stimulation of phagocytosis upon release by macrophages ... The protein encoded by this gene is an aminopeptidase involved in trimming HLA class I-binding precursors so that they can be ... the name accepted by the Hugo Gene Nomenclature Committee ER aminopeptidase associated with antigen processing (ERAAP) in mice ...
HLA constitutes a group of cell surface antigens also known as the MHC of humans. Because HLA genes are located at adjacent ... Table 3 shows association of HLA-B27 with ankylosing spondylitis among a Dutch population. Relative risk x {\displaystyle x} of ... alleles at antigen i to be x, and at antigen j to be y, the observed frequency of haplotype xy is o [ h f x y ] = d N {\ ... The HLA antigen frequency among patients exceeds more than that among a healthy population. This is evaluated by δ {\ ...
In this system, antigens were eventually assigned letters and numbers (e.g., HLA-B27 or, shortened, B27). A parallel system ... It has been shown that high resolution HLA typing (HLA-A, HLA-B, HLA-C, HLA-DRB1, HLA-DQB1 and HLA-DPB1) may be relevant in ... There are 3 major and 3 minor MHC class I genes in HLA. Major MHC class I HLA-A HLA-B HLA-C Minor genes are HLA-E, HLA-F and ... HLA-DP α-chain encoded by HLA-DPA1 locus β-chain encoded by HLA-DPB1 locus HLA-DQ α-chain encoded by HLA-DQA1 locus β-chain ...
... its relationship to HLA-B27, and the development of ankylosing spondylitis. These findings are now included in the juvenile ... He studied the significance of factor VIII related antigen in juvenile dermatomyositis. In addition, he studied the ... An association between HLA-A2 and juvenile rheumatoid arthritis in girls. J Rheumatol. 1982;9(6):916-920. Rosenberg AM, Petty ... He and colleagues first described the association of HLA-A2 with juvenile idiopathic arthritis. Along with Alan Rosenberg, he ...
In this system, antigens were eventually assigned letters and numbers (e.g., HLA-B27 or, shortened, B27). A parallel system ... "Frequencies of HLA-A, HLA-B, HLA-DR, and HLA-DQ phenotypes in the United Arab Emirates population". Tissue Antigens. 66 (2): ... HLA-C. Minor genes are HLA-E, HLA-F and HLA-G. β2-microglobulin binds with major and minor gene subunits to produce a ... HLA-DR *α-chain encoded by HLA-DRA locus. *4 β-chains (only 3 possible per person), encoded by HLA-DRB1, DRB3, DRB4, DRB5 loci ...
Ahn JK, Park YG (October 2007). "Human leukocyte antigen B27 and B51 double-positive Behçet uveitis". Arch. Ophthalmol. 125 (10 ... A large number of serological studies show a linkage between the disease and HLA-B51.[17] HLA-B51 is more frequently found from ... and symptoms appear to be milder when HLA-B27 is present.[19] At the current time, a similar infectious origin has not yet been ... Ohno S, Ohguchi M, Hirose S, Matsuda H, Wakisaka A, Aizawa M (September 1982). "Close association of HLA-Bw51 with Behçet's ...
... (A*02) is a human leukocyte antigen serotype within the HLA-A serotype group. The serotype is determined by the ... A2-B16 A2-B44 A2-B45 A2-B18 A2-B27 A2-B35 A2-B37 A2-B39 (Node in North American Amerinds) A2-B40 A2-B60 A2-B61 A2-B46 A2-Cw5- ... Before this revision, HLA-A*02 was also referred to as HLA-A2, HLA-A02, and HLA-A*2. HLA-A*02 is one particular class I major ... Tissue Antigens. 11 (2): 96-112. doi:10.1111/j.1399-0039.1978.tb01233.x. PMID 77067. "HLA Nomenclature @ hla.alleles.org". ...
Ahn JK, Park YG (October 2007). "Human leukocyte antigen B27 and B51 double-positive Behçet uveitis". Arch. Ophthalmol. 125 (10 ... Ohno S, Ohguchi M, Hirose S, Matsuda H, Wakisaka A, Aizawa M (September 1982). "Close association of HLA-Bw51 with Behçet's ... An involvement of Th1 immunoreactivity against Streptococcus Sanguinis antigen". J. Invest. Dermatol. 126 (7): 1534-40. doi: ...
The most notable and consistent is the association between HLA B27 and spondyloarthropathies like ankylosing spondylitis and ... any antibody produced against this antigen (which mimics the self-antigens) can also, in theory, bind to the host antigens, and ... Molecular mimicry - An exogenous antigen may share structural similarities with certain host antigens; thus, ... HLA DR3 is correlated strongly with Sjögren syndrome, myasthenia gravis, SLE, and DM Type 1. HLA DR4 is correlated with the ...
This is owing to the high frequency of the HLA-B27 gene in the white population.[8][9] It can occur in epidemic form. Patients ... See also: List of human leukocyte antigen alleles associated with cutaneous conditions ... A blood test for the genetic marker HLA-B27 may also be performed. About 75 percent of all the patients with Reiter's arthritis ... Reactive arthritis is associated with the HLA-B27 gene on chromosome 6 and by the presence of enthesitis as the basic ...
This is owing to the high frequency of the HLA-B27 gene in the white population. It can occur in epidemic form. Patients with ... either by an autoimmune response involving cross-reactivity of bacterial antigens with joint tissues or by bacterial antigens ... A blood test for the genetic marker HLA-B27 may also be performed. About 75 percent of all the patients with reactive arthritis ... Reactive arthritis is associated with the HLA-B27 gene on chromosome 6 and by the presence of enthesitis as the basic ...
"Leukocyte receptor complex-encoded immunomodulatory receptors show differing specificity for alternative HLA-B27 structures". ... Tissue Antigens. 64 (3): 215-25. doi:10.1111/j.0001-2815.2004.00290.x. PMID 15304001. Tedla N, An H, Borges L, Vollmer-Conna U ... Tissue Antigens. 77 (4): 305-16. doi:10.1111/j.1399-0039.2011.01633.x. PMID 21388353. Pilsbury LE, Allen RL, Vordermeier M ( ... "LILRA3 binds both classical and non-classical HLA class I molecules but with reduced affinities compared to LILRB1/LILRB2: ...
The most notable and consistent is the association between HLA B27 and spondyloarthropathies like ankylosing spondylitis and ... any antibody produced against this antigen (which mimics the self-antigens) can also, in theory, bind to the host antigens, and ... Molecular Mimicry - An exogenous antigen may share structural similarities with certain host antigens; thus, ... T-Cell-B-Cell discordance - A normal immune response is assumed to involve B and T cell responses to the same antigen, even if ...
A version of the HLA-B gene called HLA-B27 increases the risk of developing ankylosing spondylitis. It is uncertain how HLA-B27 ... HLA-B is part of a family of genes called the human leukocyte antigen (HLA) complex. The HLA complex helps the immune system ... HLA-B*45ZJ, HLA-B-3506, HLA-B-3905, HLA-B-5502, HLA-B-5602, HLA-B15, HLA-B39, HLA-B49, HLA-B50, HLA-B55, HLA-B59, HLA-B61, HLA- ... at least 28 very similar alleles are subtypes of HLA-B27. These subtypes are designated as HLA-B*2701 to HLA-B*2728. ...
Certain HLA allotypes have been found to determine the progression of HIV to AIDS; an example is the HLA-B57 and HLA-B27 ... Since NK cells recognize target cells when they express nonself HLA antigens (but not self), autologous (patients' own) NK cell ... HLA-A, HLA-B, HLA-C) and nonclassical Mamu-G (HLA-G) in primates. Some KIRs are specific for certain HLA subtypes. Most KIRs ... The selective retention of HLA-E (which is a ligand for NK cell inhibitory receptor NKG2A) and HLA-G (which is a ligand for NK ...
... of individuals with psoriatic arthritis have the HLA-B27 genotype.[3] The condition is less common in people of Asian or ... See also: List of human leukocyte antigen alleles associated with cutaneous conditions ... associations have been identified in a genome-wide association study of psoriasis and psoriatic arthritis including HLA-B27.[8] ...
For terminology help see: HLA-serotype tutorial) B7, previously HL-A7, was one of the first 'HL-A' antigens recognized, largely ... In Croatian children, two HLA-B27 alleles were found associated with disease, B*2702, B*2705. The study showed also B*0702 in ... HLA-B7 (B7) is an HLA-B serotype. The serotype identifies the more common HLA-B*07 gene products. ( ... A more recent study looked at a number of linked gene-alleles and found I82-2:D6S265-1:HLA-A3:D6S128-2:HLA-F1:D6S105-8 was ...
Certain HLA allotypes have been found to determine the progression of HIV to AIDS; an example is the HLA-B57 and HLA-B27 ... Infusions of T cells engineered to express a chimeric antigen receptor (CAR) that recognizes an antigen molecule on leukemia ... HLA-A, HLA-B, HLA-C) and nonclassical Mamu-G (HLA-G) in primates. Some KIRs are specific for certain HLA subtypes. Most KIRs ... Trophoblast cells downregulate HLA-A and HLA-B to defend against cytotoxic T cell-mediated death. This would normally trigger ...
"Nomenclature for factors of the HLA system, 2010". Tissue Antigens. 75 (4): 291-455. doi:10.1111/j.1399-0039.2010.01466.x. PMC ... HLA-B sequences shows a segment of 228 bp identical with B44 in the alpha 1 domain and a segment of 218 bp identical with B27 ... HLA-B47 (B47) is an HLA-B serotype. The serotype identifies the HLA-B*47 gene products (B*4701, B*4702, B*4703) . Comparison of ... The CYP21 gene is located close to B47 between HLA-B and HLA-DRB1 locus. Marsh, S. G.; Albert, E. D.; Bodmer, W. F.; Bontrop, R ...
They have an increased incidence of HLA-B27, as well as negative rheumatoid factor and ANA. Enthesopathy is also sometimes ... Shankarkumar U, Devraj JP, Ghosh K, Mohanty D (2002). "Seronegative spondarthritis and human leucocyte antigen association". Br ... or HLA-B27 plus 2 other SpA features Sacroiliitis on imaging: Active (acute) inflammation on MRI highly suggestive of SpA- ... They are in relation to HLA-B27 Inflammatory axial arthritis, generally sacroiliitis and spondylitis Oligoarthritis, generally ...
The protein is called human leukocyte antigen B27 (HLA-B27). ... HLA-B27 is a blood test to look for a protein that is found on ... The protein is called human leukocyte antigen B27 (HLA-B27).. Human leukocyte antigens (HLAs) are proteins that help the bodys ... Human leukocyte antigen B27; Ankylosing spondylitis-HLA; Psoriatic arthritis-HLA; Reactive arthritis-HLA ... If you have symptoms or signs of spondyloarthritis, a positive HLA-B27 test may help confirm the diagnosis. However, HLA-B27 is ...
A test indicating that HLA-B27 is present means that the patient might have a risk of... ... HLA-B27 is a human leukocyte antigen that helps the body differentiate its own cells from foreign substances. ... Patients may get an HLA-B27 antigen test to find the cause of joint swelling and pain. Another reason for an HLA test is to see ... HLA-B27 is a human leukocyte antigen that helps the body differentiate its own cells from foreign substances. A test indicating ...
Genetic modulation of antigen presentation by HLA-B27 molecules.. L Pazmany, S Rowland-Jones, S Huet, A Hill, J Sutton, R ... Genetic modulation of antigen presentation by HLA-B27 molecules.. L Pazmany, S Rowland-Jones, S Huet, A Hill, J Sutton, R ... None of the eight B cell lines that expressed HLA-B27 presented a known peptide epitope to CTL. However, cells from a family ... These data are compatible with the presence of a factor(s), possibly HLA linked, interfering with antigen presentation by ...
HLA Class I Histocompatibility Antigen, B-27 alpha Chain*HLA Class I Histocompatibility Antigen, B-27 alpha Chain ... "HLA-B27 Antigen" by people in Harvard Catalyst Profiles by year, and whether "HLA-B27 Antigen" was a major or minor topic of ... "HLA-B27 Antigen" is a descriptor in the National Library of Medicines controlled vocabulary thesaurus, MeSH (Medical Subject ... A specific HLA-B surface antigen subtype. Members of this subtype contain alpha chains that are encoded by the HLA-B*27 allele ...
Ankylosing spondylitis is 10 times more common among individuals with HLA-B27 compared to individuals without this antigen. ... HLA-B27 is found in 90% of patients with ankylosing spondylitis and 80% in Reiters disease. Ankylosing spondylitis affects 1 ...
B27 is common to the entire group of seronegative spondyloarthropathies, and assessment of HLAB27 is of diagnostic importance ... B27 monoclonal antibodies. Cross‐reactivities of anti‐HLAB27 with other HLA‐B antigens have been reported. The authors ... B27 screening: Cross‐reactivity patterns of commercially available anti HLAB27 monoclonal antibodies with other HLA‐B antigens ... HLAB27 Kit (BD Biosciences; optional) containing: * Anti‐HLAB27‐FITC/CD3‐PE (clones anti‐GS145.2 and SK7, respectively) ...
Alloreactive cytolytic T cell clones with dual recognition of HLA-B27 and HLA-DR2 antigens. Selective involvement of CD8 in ... Alloreactive cytolytic T cell clones with dual recognition of HLA-B27 and HLA-DR2 antigens. Selective involvement of CD8 in ... HLA-B27- responder cells were stimulated in vitro with HLA-B27.1+ lymphoblastoid cell lines, and alloreactive CTL clones were ... The results indicate that these clones display dual recognition for HLA-B27.1 and for HLA-DR2 and suggest that HLA-B27.1 may ...
Furthermore, ankylosing spondylitis was found more frequently in ulcerative colitis bearing HLA-B27 compared with non-B27 ... There was no significant difference in antigen frequency between patients and controls. However, the incidence of HLA-B27 was ... Histocompatibility (HLA) antigen phenotypes have been studied in 100 patients with ulcerative colitis, 100 with Crohns disease ... In addition, 12 of 14 ulcerative colitis patients and five out of six Crohns patients with HLA-B27 had total colitis, compared ...
Im wondering if its possible to impute the presence of the HLA-B27 antigen from a 23andMe genome? This is a genome of ... I just want to predict the presence or absence of the HLA-B*27 antigen (possibly HLA-B*5101 as well). ... grep HLA_B_2705 1958BC_IMPUTED.bgl.phased M HLA_B_2705 A A A A A A A A A P A A A A A A A A A A you see that the 5th individual ... So my questions are: Is it at all possible to detect the presence of HLA-B27 from a 23andMe genome using a reference panel (I ...
Rat Leucocyte Antigen B27 ELISA ELISA, HLAB27 \ E02H1251 for more molecular products just contact us ... Rat Leucocyte Antigen B27 ELISA ELISA, HLAB27 / Product Detail : E02H1251 Rat Leucocyte Antigen B27 ELISA ELISA, HLAB27. ... Rat Leucocyte Antigen B27 ELISA ELISA, HLAB27. Related products : Rat Leucocyte Antigen B27 ELISA ELISA, HLAB27 ... Rat Leucocyte Antigen B27 ELISA ELISA, HLAB27 antibody storage GENTAUR recommends for long therm storage to freeze at -24 C. ...
... blocked complement-dependent cytolysis mediated by human HLA typing sera specific for B7 and B27. Competitive binding studies ... but no difference in B7 and B27 binding could be detected by Scatchard analysis. No distinction could be made between B27 on ... Binding to B27 appeared to be slightly stronger than to B7 and stronger than to B22 in an indirect binding assay, ... This reagent will be useful in further analysis of the relationship between B27 and ankylosing spondylitis. ...
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... On-line free medical diagnosis assistant. Ranked list of possible diseases from either several symptoms or a ... Hla-b27 antigen. A specific HLA-B surface antigen subtype. Members of this subtype contain alpha chains that are encoded by the ...
HLA-B27 prevalence in all SpA ranged from 13.87% (Lebanon) to 69.43% (Kuwait). Peripheral SpA was less associated with HLA-B27 ... HLA-B27 testing method. The prevalence of HLA-B27 in the normal population is significantly lower in the Middle Eastern and ... HLAB27 prevalence in the normal population ranged from 0.3% (Oman) to 6.8% (Turkey). HLA-B27 prevalence in AxSpA ranged from ... Therefore, HLA-B27 is a central feature in SpA classification and plays a pivotal role in referral strategies and early ...
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Chromosome 6 contains locus HLA-A, HLA-B, HLA-C, HLA-DM, HLA-DOA, HLA-DOB, HLA-DP, HLA-DQ, and HLA-DR which encode Human ... the presence of HLA-B27 supports the diagnosis of Reiter syndrome. HLA-B27 is found in 5% to 7% of normal patients. ... If the HLA antigens of the donor are not compatible with the recipient, the recipient will make antibodies to those antigens, ... The HLA system has also been used to assist in the diagnosis of certain other diseases, some types of Human Lymphocyte Antigens ...
Treatment HLA-B27 antigen. Symptoms and causes HLA-B27 antigen Prophylaxis HLA-B27 antigen ... The protein is called human leukocyte antigen B27 (HLA-B27).. Human leukocyte antigens (HLAs) are proteins that help the bodyâ ... A normal (negative) result means HLA-B27 is absent.. However, HLA-B27 is found in 5 - 7% of Caucasians who do not have an ... HLA antigen testing is also used to match donated tissue to organ recipients. For example, it may be done when a person needs a ...
HLA-B27 Antigen. Does this test have other names?. Human lymphocyte antigen B27, human leukocyte A antigen, histocompatibility ... A negative result means you dont have HLA-B27 in your blood. A positive result means HLA-B27 was found in your blood. You may ... If your HLA-B27 antigen test is positive, you may need other tests to help confirm a diagnosis of an autoimmune disease. You ... This test looks for HLA-B27, which are proteins called antigens. These are found on the surface of white blood cells that are ...
The HLA B27-positive patients contracted amyloidosis on average 5.9 (median 6.7) years earlier than the HLA B27-negative ... The HLA B27-positive patients underwent arthroplasty on average 2.9 (median 3.5) years earlier than the HLA B27-negative ... two out of 15 in remission were HLA B27 positive, whereas as many as 41 out of 86 with still active disease were HLA B27 ... HLA B27: a prognostic factor in juvenile chronic arthritis.. Savolainen HA1, Lehtimäki M, Kautiainen H, Aho K, Anttila P. ...
An HLA-B27-positive patient is more likely to exhibit spondyloarthropathies than an HLA-B27-negative person.. ... There is a strong association between the presence of HLA-B27 antigen and an increased incidence of ankylosing spondylitis as ... HLA B27 ANTIGEN at Geisinger Medical Laboratories ...
HLA antigens in ankylosing spondylitis: the association of HLA-B27. HLA antigens in ankylosing spondylitis: the association of ... Adolescent , Adult , Female , HLA Antigens/analysis , Humans , Male , Middle Aged , Spondylitis, Ankylosing/genetics ... HLA Antigens / Middle Aged Language: English Year: 1980 Type: Article ... HLA Antigens / Middle Aged Language: English Year: 1980 Type: Article ...
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27 foo2hla.bgl.phased M HLA_B_27 A A M HLA_B_2705 A A This also says that HLA-B27 is A. bsent in both chromosomes for HLA-B27 ... HLA_B_27 P A 0,002 0,110 0,888 foo2hla.bgl.gprobs:HLA_B_2705 P A 0,002 0,110 0,888 The first line is for HLA-B27 in general, ... Imputing the presence of the HLA-B27 antigen using your 23andMe genome By Christian Stigen Larsen 23 Feb 2016 ... Recently, I wanted to see if I could impute the presence of the HLA-B27 antigen using my raw 23andMe DNA data. This is a pretty ...
... to reconcile the apparent differences in the reported frequency of ankylosing spondylitis and radiological sacroiliitis in HLA- ... B27 positive individuals. Pelvic radiographs from 125 Busselton subjects were mixed with 81 other films selected to illustrate ... Observer variation in grading sacroiliac radiographs in HLA-B27 positive individuals J Rheumatol. 1983 Apr;10(2):247-54. ... HLA-B27 Antigen * Humans * Individuality * Radiography * Sacroiliac Joint / diagnostic imaging* * Spondylitis, Ankylosing / ...
HLA-B27 positive acute anterior uveitis is the most common type of uveitis, and it is an autoimmune disease that can be ... HLA-B27 Antigen / immunology* * Humans * Immunologic Factors / immunology * Inflammation Mediators / immunology* * Iris / ... HLA-B27 Positive Acute Anterior Uveitis - A Translational Perspective] Klin Monbl Augenheilkd. 2017 May;234(5):652-656. doi: ... Background HLA-B27 positive acute anterior uveitis is the most common type of uveitis, and it is an autoimmune disease that can ...
Male members showed a preponderance over females in HLA B27 positivity. Detection of HLA B27 could help in the diagnosis of AS ... Adult , Age Factors , Autoimmune Diseases/diagnosis , Biomarkers/analysis , Female , HLA-B27 Antigen/analysis , Humans , ... Association of HLA B27 antigen in Indian patients of ankylosing spondylitis and other autoimmune diseases. ... Association of HLA B27 antigen in Indian patients of ankylosing spondylitis and other auto ...
The cell-surface antigen HLA-B27 is well k ... 0/HLA-B27 Antigen From MEDLINE®/PubMed®, a database of the U.S ... RESULTS: HLA-B27 was found to be positive in 25.5% of the patients, and we found a higher proportion of HLA-B27-positive boys ... HLA-B27 Antigen / genetics*. Humans. Longitudinal Studies. Male. Norway. Sex Factors. Sweden. ... The cell-surface antigen HLA-B27 is well known to be associated with spondyloarthropathies, reactive arthritis, and enthesitis ...
HLA-B27 Antigen. LinkOut - more resources. Full Text Sources. *Wiley. Other Literature Sources. *Cited by Patents in - The Lens ... The presence of HLA-B40 in HLA-B27 positive individuals increased the risk for disease more than 3-fold, confirming previous ... In a second analysis in which the population association of the MHC gene HLA-B27 with AS was taken into account, the maximal ... To assess the hypothesis that B27 or a gene(s) in close proximity (e.g., within or near the major histocompatibility complex [ ...
Clinical Findings of Ankylosing Spondylitis With and Without Human Leukocyte Antigen (HLA)-B27 and HLA-B51. In: Annals of the ... Clinical Findings of Ankylosing Spondylitis With and Without Human Leukocyte Antigen (HLA)-B27 and HLA-B51. Annals of the ... Clinical Findings of Ankylosing Spondylitis With and Without Human Leukocyte Antigen (HLA)-B27 and HLA-B51. / Jung, Jae Hyun; ... title = "Clinical Findings of Ankylosing Spondylitis With and Without Human Leukocyte Antigen (HLA)-B27 and HLA-B51", ...
Still, the pathogenic role of HLA-B27 molecules in AS is elusive. Here, we focus on the biology of HLA-B27 from the genetics to ... This is due to the high capacity of virus-specific, HLA-B27-restricted CD8+ T cells for both intrinsic (i.e. polyfunctionality ... In particular, an extensive comparison of HLA-B*2705 and B*2709 molecules, that differ in a single amino acid (Asp116 to His116 ... It is tempting to speculate that these two aspects are not independent and that the association of B27 molecules to ...
  • Conditions such as arthritis, ankylosing spondylitis and inflammation of the sacroiliac joint may cause an increase in HLA-B27 and human leukocyte antigens, states MedlinePlus. (reference.com)
  • HLA-B27 is found in 90% of patients with ankylosing spondylitis and 80% in Reiter's disease. (specialtylabs.com)
  • Ankylosing spondylitis is 10 times more common among individuals with HLA-B27 compared to individuals without this antigen. (specialtylabs.com)
  • However, the incidence of HLA-B27 was increased in the patients complicated by ankylosing spondylitis and/or sacroiliitis in both ulcerative colitis and Crohn's disease. (bmj.com)
  • Furthermore, ankylosing spondylitis was found more frequently in ulcerative colitis bearing HLA-B27 compared with non-B27 patients (P less than 0-01). (bmj.com)
  • No distinction could be made between B27 on cells from normal and from ankylosing spondylitis patients in any assay system. (semanticscholar.org)
  • HLA type B27 is associated with autoimmune diseases such as ankylosing spondylitis and Reiter syndrome. (drugline.org)
  • There is a strong association between the presence of HLA-B27 antigen and an increased incidence of ankylosing spondylitis as well as several other disorders, such as Reiter's syndrome, psoriatic arthritis, and arthroplasties associated with inflammatory bowel diseases. (geisingermedicallabs.com)
  • HLA antigens in ankylosing spondylitis: the association of HLA-B27. (bvsalud.org)
  • This study attempts to reconcile the apparent differences in the reported frequency of ankylosing spondylitis and radiological sacroiliitis in HLA-B27 positive individuals. (nih.gov)
  • Association of HLA B27 antigen in Indian patients of ankylosing spondylitis and other autoimmune diseases. (bvsalud.org)
  • One thousand three hundred and forty clinically suspected patients of Ankylosing Spondylitis (AS) and other autoimmune diseases and 5000 controls were studied to detect the association of HLA B27 antigen amongst them. (bvsalud.org)
  • To assess the hypothesis that B27 or a gene(s) in close proximity (e.g., within or near the major histocompatibility complex [MHC]) represents a disease-causing ankylosing spondylitis (AS) gene, and therefore contributes directly to the pathogenesis of this disorder. (nih.gov)
  • INTRODUCTION: Ankylosing spondylitis (AS) and Behçet's disease are known to be associated with the human leukocyte antigen (HLA)-B27 and HLA-B51 genes, respectively. (elsevier.com)
  • The HLA-B27 is historically studied as a susceptibility factor in spondyloarthropathies and, primarily, in ankylosing spondylitis (AS). (archives-ouvertes.fr)
  • Association of the HLA-B27 antigen and the CTLA4 gene CT60/rs3087243 polymorphism with ankylosing spondylitis in Algerian population: A case-contro. (cdc.gov)
  • High spondylitis risk was detected for HLA-B27 allele (OR= 14.62, p = 10 -6 ) in addition to a significant association of the CT60*G allele (OR= 1.89, p = .002). (cdc.gov)
  • Interestingly, the CT60*G allele revealed an increased spondylitis risk in the B27 negative group (OR= 2.81, p = .006). (cdc.gov)
  • The present work showed in West Algerian population that the HLA-B27 antigen and the variation in the CTLA4 3'UTR region played an important role in the ankylosing spondylitis susceptibility. (cdc.gov)
  • Only 1 in 20 people who are HLA B27+ develop ankylosing spondylitis. (chop.edu)
  • Ankylosing spondylitis (AS), psoriatic arthritis (PsA), and SAPHO syndrome (SAPHO) are seronegative spondyloarthropathies (SpA) which are connected with the presence of HLA-B27 antigen [ 1 - 3 ]. (hindawi.com)
  • Consider HLA-B27 testing, particularly if a patient complains of chronic lower back pain (i.e., ankylosing spondylitis) or plantar heel pain. (aao.org)
  • in there you will find a Supplemental Table that includes a list of SNPs and alleles that can be used to predict the classical HLA alleles, as well as r2 values between SNP alleles and HLA alleles. (biostars.org)
  • We analysed whether HLA-A, HLA-B and HLA-DRB1 alleles could associate with these patterns. (bmj.com)
  • Results Only HLA-B27 and HLA-B15 alleles were associated with SpA. (bmj.com)
  • The two other alleles were HLA.DRB4*01 at 35.7% and HLA-DQB1*0501 at 28.6%, as detected in each of the clinical and radiological manifestations. (scielo.org.co)
  • The alleles HLA-B27, HLA-DRB4*01 and HLA-DQB1*0501 were common in the different subtypes of spondyloarthritis and were frequent in the specific clinical axial, peripheral and extraarticular clinical manifestations, as well as radiological sacroiliitis. (scielo.org.co)
  • 2013) Co-expression of HLA-B7 and HLA-B27 alleles is associated with B7-restricted immunodominant responses following influenza infection. (miltenyibiotec.com)
  • If you have symptoms or signs of spondyloarthritis, a positive HLA-B27 test may help confirm the diagnosis. (medlineplus.gov)
  • Increased expression of human leucocyte antigen class I free heavy chains on monocytes of patients with spondyloarthritis and cells transfected with HLA-B27. (semanticscholar.org)
  • Current Assessment of Spondyloarthritis International Society (ASAS) classification criteria of AxSpA rely either on sacroiliitis on imaging plus one SpA feature or positive HLAB27 antigen plus two SpA features, in a patient with chronic low back pain and age at onset of less than 45 years. (biomedcentral.com)
  • Objective Human leucocyte antigen (HLA) B27 and HLA-B15 are associated with spondyloarthritis (SpA). (bmj.com)
  • Human leucocyte antigen (HLA) HLA-B15 has been associated with spondyloarthritis (SpA) and oligoarthritis in different populations. (bmj.com)
  • The frequency of HLA class I and II was determined in a population of patients with spondyloarthritis with respect to detection in the clinical setting and by radiology. (scielo.org.co)
  • There are several arguments favouring a role for haematopoietic cells in the pathophysiology of spondyloarthritis, including HLA-B27-associated dendritic cell disturbances, HLA-B27 misfolding properties and T helper 17 cells. (biomedcentral.com)
  • The data suggest that B27 histocompatibility antigen could be a pathogenetic discriminator between the arthropathies in IBD and may be of prognostic significance with respect to extension and severity of the disease. (bmj.com)
  • In a second analysis in which the population association of the MHC gene HLA-B27 with AS was taken into account, the maximal LOD score was 7.5 at O = 0.05. (nih.gov)
  • The aim of this study was to investigate the differences in clinical findings of AS patients with and without the HLA-B27 gene. (elsevier.com)
  • however, other clinical findings did not differ significantly according to the absence or presence of the HLA-B27 gene. (elsevier.com)
  • In this study, we were interested in evaluating the HLA-B27 frequency and in exploring the CTLA4 gene in a sample of the North African population. (cdc.gov)
  • A gene marker called HLA-B27 is found in almost all Caucasians and half of of African Americans who have the disease. (ahealthyme.com)
  • Most people with AS are positive for the HLA-B27 gene. (medlineplus.gov)
  • Hla gene? (healthtap.com)
  • One gene, human leukocyte antigen ( HLA ) B27, increases a person's chance of developing reactive arthritis. (nih.gov)
  • However, inheriting the HLA B27 gene does not necessarily mean you will get reactive arthritis. (nih.gov)
  • The serotype identifies the more common HLA-B*51 gene products. (wikipedia.org)
  • HLA‐B27 is common to the entire group of seronegative spondyloarthropathies, and assessment of HLA‐B27 is of diagnostic importance if any of these diseases are considered. (currentprotocols.com)
  • An HLA-B27-positive patient is more likely to exhibit spondyloarthropathies than an HLA-B27-negative person. (geisingermedicallabs.com)
  • The cell-surface antigen HLA-B27 is well known to be associated with spondyloarthropathies, reactive arthritis, and enthesitis. (biomedsearch.com)
  • It is tempting to speculate that these two aspects are not independent and that the association of B27 molecules to autoimmunity is the downside of this superior functional efficacy which, in given genetic backgrounds and environmental conditions, can support a chronic inflammation leading to spondyloarthropathies. (archives-ouvertes.fr)
  • The BD™ HLA-B27 system works with the BD FACSCanto II system to provide a complete solution for rapid detection of HLA-B27 antigen, which is clinically relevant to the evaluation of seronegative spondyloarthropathies. (bdbiosciences.com)
  • Human leucocyte antigen (HLA)-B27 is strongly associated with spondyloarthropathies, including reactive arthritis. (csic.es)
  • HLA-B27 is known for its strong association with inflammatory spondyloarthropathies. (miltenyibiotec.com)
  • Background HLA-B27 positive acute anterior uveitis is the most common type of uveitis, and it is an autoimmune disease that can be triggered by infections. (nih.gov)
  • The goal is to establish new markers for individual susceptibility in the risk group of the HLA-B27 positive population, because only about 1% of the HLA-B27 positive population will eventually develop acute anterior uveitis. (nih.gov)
  • Patients suffering from other autoimmune diseases such as rheumatoid arthritis , psoriasis , Reiter's syndrome and uveitis and patients with inflammatory bowel disease , colitis , eczema , bacillary or fungal infection were also found to be HLA B27 positive. (bvsalud.org)
  • CONCLUSION: HLA-B27 was associated with the development of uveitis but not with other clinical findings or disease activity in AS patients. (elsevier.com)
  • The following data were recorded: age, sex, disease duration, and extra-articular symptoms: acute anterior uveitis (AAU), inflammatory bowel disease (IBD), skin psoriasis, psoriatic onychopathy, and positivity for HLA B27. (hindawi.com)
  • HLA-B27 acts as an independent predisposing factor: it was present in 22% of patients with their first attack of acute uveitis compared with 51% of patients with recurrent disease. (docme.ru)
  • This is exemplified by the close association of HLA-B27 with anterior uveitis (1) and, similarly, by a well-recognized association of HLA-B5 in BehGet's syndrome, complicated by uveitis or retinal vasculitis in Japanese and Turkish populations (2). (docme.ru)
  • Recent evidence has also implicated HLA-DRS in the chronic anterior uveitis of juvenile polyarthritis (3). (docme.ru)
  • The results demonstrated that alphaj-antitrypsin-deficient phenotypes substantially predispose humans to chronic, bilateral, and recurrent anterior WAKEFIELD ET AL 1432 uveitis (severe uveitis), while HLA-B27 appears to act as an independent genetic predisposing factor in recurrent anterior uveitis. (docme.ru)
  • Patients with human leucocyte antigen-B27 (HLA-B27) uveitis develop anterior segment inflammation with occasional spillover into the anterior vitreous. (aao.org)
  • Members of this subtype contain alpha chains that are encoded by the HLA-B*27 allele family. (harvard.edu)
  • In the first category the frequency of the HLA B27 allele was compared between those who had attained remission and those who had not. (nih.gov)
  • bsent in both chromosomes for HLA-B27 and the HLA-B2705 allele. (csl.name)
  • The frequency of HLA-B27 was 50% overall, and it was the most frequent allele. (scielo.org.co)
  • HLA B27: a prognostic factor in juvenile chronic arthritis. (nih.gov)
  • This study was performed to assess the frequency of HLA B27 in patients with juvenile chronic arthritis (JCA) of varying severity and outcome by studying three patient categories: those in whom cytostatic treatment with azathioprine had been started, those with secondary amyloidosis, and those with arthroplasty of the knee or hip joints. (nih.gov)
  • HLA-B27 predicts a more extended disease with increasing age at onset in boys with juvenile idiopathic arthritis. (biomedsearch.com)
  • HLA-B27 plays an important role in the classification of JIA, since evidence of sacroiliitis most often evolves after years of arthritis in other joints. (biomedsearch.com)
  • B. Arthritis in HLA B27 positive males beginning after the age of 6 years. (medicalcriteria.com)
  • In studies of antigenic peptide presentation, we have found a healthy volunteer whose lymphoblastoid cells were unable to present three different virus-derived epitopes to cytotoxic T lymphocytes (CTL) despite expressing the correct restricting HLA-B27 molecules on the cell surface. (rupress.org)
  • None of the eight B cell lines that expressed HLA-B27 presented a known peptide epitope to CTL. (rupress.org)
  • However, cells from a family member that expressed HLA-B8 could present an epitope peptide restricted by that molecule. (rupress.org)
  • The B27 molecule in this family proved to be the B2702 subtype on isoelectric focusing gels, appearing in exactly the same position as B2702 from other cell lines that did present the peptide. (rupress.org)
  • The cloned cDNA was transfected into HLA-A- and B-negative HMy/C1R cells, and the B2702 molecules generated in this environment rendered these cells, after incubation with peptide, susceptible to lysis by peptide-specific CTL. (rupress.org)
  • Dominant role of the ERAP1 polymorphism R528K in shaping the HLA-B27 Peptidome through differential processing determined by multiple peptide residues. (semanticscholar.org)
  • Here, we focus on the biology of HLA-B27 from the genetics to the biochemistry and to the structural/dynamical properties of B27:peptide complexes as obtained from atomistic molecular dynamics simulation. (archives-ouvertes.fr)
  • In particular, an extensive comparison of HLA-B*2705 and B*2709 molecules, that differ in a single amino acid (Asp116 to His116) and are differentially associated with AS, indicates that position 116 is crucial for shaping the entire peptide-presenting groove. (archives-ouvertes.fr)
  • Mutational analysis of two constrained peptides (the HLA B44 restricted self-peptide (B44DPα-EEFGRAFSF) and an H2-D b restricted influenza peptide (D b PA, SSLENFRAYV) demonstrated that the conformation of the prominently exposed arginine in both peptides was governed by interactions with MHC-I-orientated flanking residues from the peptide itself. (pnas.org)
  • A positive test means HLA-B27 is present. (medlineplus.gov)
  • A positive result means there is more HLA-B27 in the blood than there should be. (reference.com)
  • However, HLA-B27 testing can be useful for AxSpA positive diagnosis, given the high OR. (biomedcentral.com)
  • In the presence of suggestive clinical findings, a positive HLA-B27 test may confirm the diagnosis. (drugline.org)
  • If your HLA-B27 antigen test is positive, you may need other tests to help confirm a diagnosis of an autoimmune disease. (gundersenhealth.org)
  • A positive result means HLA-B27 was found in your blood. (gundersenhealth.org)
  • If you are white, you are more likely to test positive for the HLA-B27 antigens. (gundersenhealth.org)
  • In the second and third categories the rate at which amyloidosis developed and the timing for the need of arthroplasty, were compared for HLA B27-positive and -negative patients. (nih.gov)
  • Ten of the 37 patients in the control group (27%) were HLA B27 positive as opposed to 84 out of 190 (44%) in the three study groups. (nih.gov)
  • Of the 101 patients treated with azathioprine, two out of 15 in remission were HLA B27 positive, whereas as many as 41 out of 86 with still active disease were HLA B27 positive (p = 0.013). (nih.gov)
  • The HLA B27-positive patients contracted amyloidosis on average 5.9 (median 6.7) years earlier than the HLA B27-negative patients (p = 0.038). (nih.gov)
  • The HLA B27-positive patients underwent arthroplasty on average 2.9 (median 3.5) years earlier than the HLA B27-negative patients (p = 0.050). (nih.gov)
  • We conclude that HLA B27-positive cases are accumulated among the most severe cases of JCA. (nih.gov)
  • The presence of HLA-B40 in HLA-B27 positive individuals increased the risk for disease more than 3-fold, confirming previous reports. (nih.gov)
  • There were no significant differences in the clinical findings of patients with positive and negative HLA-B51. (elsevier.com)
  • RESULTS: HLA-B27 was found to be positive in 25.5% of the patients, and we found a higher proportion of HLA-B27-positive boys with older age at disease onset (p=0.034). (biomedsearch.com)
  • Regression analysis showed a correlation of 0.7 in the HLA-B27-positive boys, pointing to a higher risk of more joint involvement with older age at disease onset. (biomedsearch.com)
  • The haplotype HLA-B27 and DR11 antigens were positive. (thefreelibrary.com)
  • HLA-B27 was negative and HLADR11 positive. (thefreelibrary.com)
  • This means your child may test positive for HLA-B27, but not have JAS. (childrenshospital.org)
  • Moreover, 2 healthy controls, but none of our patients with isolated axSpA were positive for HLA-B15. (bmj.com)
  • You may be able to provide prognostic information if the human leukocyte antigen (HLA)-B27 result is positive. (aao.org)
  • Critical role of endoplasmic reticulum aminopeptidase 1 in determining the length and sequence of peptides bound and presented by HLA-B27. (semanticscholar.org)
  • It has been suggested that peptides derived from bacterial proteins and presented by HLA-B27 to cytotoxic T lymphocytes might show molecular mimicry with autologous peptides, leading to T-cell cross-reaction and autoimmunity. (csic.es)
  • Therefore, HLA-B27 is a central feature in SpA classification and plays a pivotal role in referral strategies and early diagnosis. (biomedcentral.com)
  • The HLA system has also been used to assist in the diagnosis of certain other diseases, some types of Human Lymphocyte Antigens are associated with conditions which are mostly believed to be genetic disorders. (wbccount.org)
  • When a patient presents with recurrent and multiple arthritic complaints, the presence of HLA-B27 supports the diagnosis of Reiter syndrome. (wbccount.org)
  • Detection of HLA B27 could help in the diagnosis of AS. (bvsalud.org)
  • The blood test marker antigen HLA-B27, when present, supports the diagnosis. (medicinenet.com)
  • In this cohort, the contribution of MRI and human leucocyte antigen B27 to purely clinical criteria in making a diagnosis of axial SpA was limited. (bmj.com)
  • Lab tests are helpful as a guide for the diagnosis like the genetic marker hla b-27. (healthtap.com)
  • The protein is called human leukocyte antigen B27 (HLA-B27). (medlineplus.gov)
  • Human leukocyte antigens (HLAs) are proteins that help the body's immune system tell the difference between its own cells and foreign, harmful substances. (medlineplus.gov)
  • Human Leukocyte Antigen (HLA) B-27 - blood. (medlineplus.gov)
  • Human leukocyte antigen: the major histocompatibility complex of man. (medlineplus.gov)
  • HLA-B27 is a human leukocyte antigen that helps the body differentiate its own cells from foreign substances. (reference.com)
  • Human leukocyte antigens help regulate the immune system, and their main role is to let the body tell the difference between normal cells and substances that it needs to attack, states MedlinePlus. (reference.com)
  • Yersinia enterocolitica serotype O:3 alters the expression of serologic HLA-B27 epitopes on human monocytes. (semanticscholar.org)
  • Dependence of the localization and function of the human cytomegalovirus protein US6 on the transporter associated with antigen processing. (semanticscholar.org)
  • Human Lymphocyte Antigens (HLAs) present the Major Histocompatability Complex of human. (wbccount.org)
  • Human Lymphocyte Antigens are encoded by the HLA region in Chromosome 6 of human DNA. (wbccount.org)
  • Human leukocyte antigens (HLAs) are proteins that help the body’s immune system tell the difference between its own cells and foreign, harmful substances. (drugline.org)
  • The Berlin criteria set for SpA that has a prominent place for MRI and human leucocyte antigen B27 was used to quantify the relative contribution of MRI in classifying SpA. (bmj.com)
  • The common genetic feature is human leucocyte antigen B27 (HLA-B27). (bmj.com)
  • It also may occur in people with autoimmune diseases linked to human leukocyte antigen B27 (HLA B27). (britannica.com)
  • Human immune-response, D-related antigen encoded by the D locus on chromosome 6 and found on lymphoid cells. (curehunter.com)
  • Human leukocyte antigen B27 (HLA-B27) is a protein you may have in your blood. (spineuniverse.com)
  • Antinuclear antibodies, C-reactive protein, riboflavin and human leukocyte antigen (HLA)-B27 were negative. (fluoridealert.org)
  • Histocompatibility human leukocyte antigen (HLA)-B27 is identified in most patients with ulcerative colitis, though this finding is not associated with the condition. (medscape.com)
  • Clone REA176 recognizes HLA B7 and B27 class I human leukocyte antigens (HLA). (miltenyibiotec.com)
  • Human peripheral blood cells after erythrocyte lysis were stained with Anti-HLA-B7, B27 antibodies or with the corresponding REA Control (S) antibodies (left images). (miltenyibiotec.com)
  • Histocompatibility antigens in inflammatory bowel disease. (bmj.com)
  • HIBAG is an HLA genotype imputation tool, HIBAG can be used by researchers with published parameter estimates ( http://www.biostat.washington.edu/~bsweir/HIBAG/ ) instead of requiring access to large training sample datasets. (biostars.org)
  • This is particularly helpful if the reputed father or child has an unusual HLA genotype. (wbccount.org)
  • A common HLA genotype in either the father or child increases the likelihood that there are many potential fathers of that child. (wbccount.org)
  • In a paternity case, if the child or father has an unusual HLA genotype, paternity could be clear. (sclhealth.org)
  • If it's a common HLA genotype, the child could have many potential fathers. (sclhealth.org)
  • Male members showed a preponderance over females in HLA B27 positivity. (bvsalud.org)
  • Routine HLA‐B27 typing by flow cytometry: Differentiation of the products of HLA‐B*2702, B*2705 and B*2708. (currentprotocols.com)
  • The association of axial SpA with HLA-B27 seems to be lower in most Arab populations compared to Western European populations [ 11 ], possibly due to genetic backgrounds. (biomedcentral.com)
  • Conclusions Our data suggest that the presence of HLA-B15 favours the development of isolated/combined peripheral rather than isolated axSpA, while HLA-B27 promotes an isolated/combined axial disease and excludes a peripheral pattern. (bmj.com)
  • HLA-B15 favours a preferably peripheral pattern while HLA-B27 promotes an isolated/combined axial disease and excludes an isolated peripheral pattern. (bmj.com)
  • Screening is hampered by the lack of true monospecific anti‐HLA‐B27 monoclonal antibodies. (currentprotocols.com)
  • If the HLA antigens of the donor are not compatible with the recipient, the recipient will make antibodies to those antigens, accelerating rejection. (wbccount.org)
  • Prior HLA sensitization causes antibodies to form in the blood of a transplant recipient and shortens the survival of red blood cells (RBCs) or platelets when transfused. (wbccount.org)
  • b) indirect infection, which may be of two types: a reactive arthropathy, where microbial infection of the body is established but neither organisms nor antigens can be identified in the joint, and a postinfective arthropathy, where microbial antigen is present but recovery of an organism is inconstant and evidence of local multiplication is lacking. (icd10data.com)
  • Their clinical significance and their association with arthropathy with special reference to HLA-B27 (W27). (bmj.com)
  • HLA-B51 was not associated with the clinical findings or disease activity of AS. (elsevier.com)
  • We investigated the associations of HLA-B27 and the clinical manifestations of JIA using a method as close to a population-based study as possible. (biomedsearch.com)
  • This unit describes screening for HLA‐B27 on peripheral blood lymphocytes using more than one HLA‐B27 monoclonal antibody to detect possible cross‐reactivity with non‐HLA‐B27 antigens. (currentprotocols.com)
  • Recognition of HLA-B27 and related antigen by a monoclonal antibody. (semanticscholar.org)
  • A monoclonal antibody that binds specifically to HLA-B27, B7, and B22 is described. (semanticscholar.org)
  • Histocompatibility (HLA) antigen phenotypes have been studied in 100 patients with ulcerative colitis, 100 with Crohn's disease, and 283 normal controls. (bmj.com)
  • The primary objective of the study is to review the prevalence of HLA-B27 in normal and AxSpA populations in Middle Eastern and Arab Countries and to assess the strength of association between HLA-B27 antigen and AxSpA. (biomedcentral.com)
  • Studies were included in the analysis if they reported prevalence of HLA-B27 in AxSpA and/or general population and if they covered geographical location in the Middle East or Arab countries in the Mediterranean basin. (biomedcentral.com)
  • HLAB27 prevalence in the normal population ranged from 0.3% (Oman) to 6.8% (Turkey). (biomedcentral.com)
  • HLA-B27 prevalence in AxSpA ranged from 26.2% (Lebanon) to 91% (Turkey). (biomedcentral.com)
  • HLA-B27 prevalence in all SpA ranged from 13.87% (Lebanon) to 69.43% (Kuwait). (biomedcentral.com)
  • Peripheral SpA was less associated with HLA-B27 than AxSpA, indicating the need of differentiating between the two entities when calculating prevalence. (biomedcentral.com)
  • The prevalence of HLA-B27 in the normal population is significantly lower in the Middle Eastern and Arab countries than in Western Countries. (biomedcentral.com)
  • This may affect the diagnostic value of HLA-B27 antigen, and impact the local application of published referral strategies, which were studied in Western populations with high HLA-B27 prevalence [ 9 ]. (biomedcentral.com)
  • However, the heterogeneity in HLA-B27 prevalence within Middle Eastern and Arab Countries [ 12 ] can be related either to true differences between the populations or to heterogeneity in the methodology of the studies. (biomedcentral.com)
  • Genetic predisposition is suggested by the higher prevalence of HLA-B27, an inherited blood marker, in patients with SAPHO syndrome. (medicinenet.com)
  • Patients were divided into 4 groups based on absence or presence of the HLA-B27 and HLA-B51 genes. (elsevier.com)
  • There is a group of genes called HLA antigens that play a major role in whether your child will get a certain disease. (childrenshospital.org)
  • However, the global distribution of HLA-B27 varies considerably and furthermore, associations with non-HLA-B27 genes have been described. (scielo.org.co)
  • However, HLA-B27 is found in some normal people and does not always mean you have a disease. (medlineplus.gov)
  • Disease progression despite protective HLA expression in an HIV-infected transmission pair. (harvard.edu)
  • If you have HLA-B27, you may have an autoimmune disease. (gundersenhealth.org)
  • CONCLUSION: HLA-B27 is of increasing importance with older age at disease onset in boys with JIA, predicting more active joints within the first 3 years of disease, and also involving small joints in the lower extremity to a greater degree than in HLA-B27-negative boys. (biomedsearch.com)
  • During the first 3 years of disease the occurrence of HLA-B27 is associated with inflammatory back pain in both sexes, but with enthesitis only in boys. (biomedsearch.com)
  • Overall, the results point at the antigen presentation as the key event in the disease pathogenesis. (archives-ouvertes.fr)
  • The heterogeneity of this disease is deduced by genetic difference found between B27+ and B27- groups. (cdc.gov)
  • If this antigen is present, it may mean there is some type of autoimmune disease. (ahealthyme.com)
  • lupus erythematosus (SLE), anti-double stranded DNA autoantibodies are features of active disease and are particularly associated with lupus nephritis, and the identity of the self or foreign antigens that "break tolerance", leading to anti-dsDNA and other autoantibody specificities, has been a topic of extensive study. (bioportfolio.com)
  • In this study, conducted on a group of Latin American patients with SpA, the presence of HLA-B15 and HLA-B27 clearly influence the pattern of presentation of the disease. (bmj.com)
  • 143 1 IMMUNOGENETIC FACTORS IN INFLAMMATORY EYE DISEASE Influence of HLA-B27 and Alpha,-Antitrypsin Phenotypes on Disease Expression DENIS WAKEFIELD, SAMUEL N. BREIT, PEGGY CLARK, and RONALD PENNY The relationship between the nature and severity of inflammatory eye disease was analyzed with respect to HLA antigens and alphal-antitrypsin phenotypes. (docme.ru)
  • In the present study we analyzed the relationship between alphal-antitrypsin phenotype, HLA antigens, and the nature and severity of inflammatory eye disease. (docme.ru)
  • HLA-B51 is found more frequently in disease that has an eye involvement. (wikipedia.org)
  • This is a pretty important antigen, because it is associated with several diseases. (csl.name)
  • There was no significant difference in antigen frequency between patients and controls. (bmj.com)
  • In addition, 12 of 14 ulcerative colitis patients and five out of six Crohn's patients with HLA-B27 had total colitis, compared with the frequency of total colitis in non-B27 patients (P less than 0-024 and less than 0-03 respectively). (bmj.com)
  • Odds ratios (OR) were calculated for each country, as a measure of the strength of association between HLA-B27 and AxSpA, compared to the normal population, using the two-by-two frequency table. (biomedcentral.com)
  • A high frequency of HLA-B27 and HLA-DRB4*01 (64.3%) was noted in patients with dactylitis. (scielo.org.co)
  • Food or bacterial antigens might exert an effect on the already damaged mucosal lining, which has increased permeability. (medscape.com)
  • The genetic risk of the HLA-B27 specificity and the CTLA4/CT60 polymorphism were assessed by odds ratios (OR) with 95% confidence intervals (CI). (cdc.gov)
  • If your child has HLA-B27, she may have a genetic susceptibility (increased chance) of developing JAS. (childrenshospital.org)
  • 1981). "Host factors and susceptibility to rubella virus infection: the association of HLA antigens" . (wikipedia.org)
  • A test indicating that HLA-B27 is present means that the patient might have a risk of developing an autoimmune disorder, notes MedlinePlus. (reference.com)
  • Typically, people of primarily Caucasian heritage have a naturally higher level of HLA-B27, notes MedlinePlus. (reference.com)
  • For this test, lymphocytes from the patient are extracted and incubated with anti-HLA-specific cytotoxic antibody. (wbccount.org)
  • Antigens binding to class I molecules are 8-10 amino acids long and play an important role in recognition of the virus infected and malignant cells by cytotoxic T lymphocytes (CTLs). (miltenyibiotec.com)
  • HLA-B7 is a risk factor for cervical cancer, sarcoidosis, and early-onset spondylarthropathies. (miltenyibiotec.com)
  • Among the alternatives to traditional typing by the complement‐dependent cytotoxicity assay, flow cytometric HLA‐B27 screening is most widely used in general diagnostic laboratories, as it is simple, rapid, and cost effective. (currentprotocols.com)
  • Binding to B27 appeared to be slightly stronger than to B7 and stronger than to B22 in an indirect binding assay, but no difference in B7 and B27 binding could be detected by Scatchard analysis. (semanticscholar.org)
  • Tests for rapid plasma reagin and microhemagglutination assay with treponemal pallidum antigen should be ordered to rule out syphilis. (aao.org)
  • In contrast, none of the 29 IBD patients with "enteropathic" peripheral arthropathy had B27 antigen. (bmj.com)
  • HLA-A29+ birdshot chorioretinopathy may present with vitreous cells in conjunction with creamy, mid-peripheral retinal lesions, retinal vasculitis and optic disc edema. (aao.org)
  • HLA-B27 is a blood test to look for a protein that is found on the surface of white blood cells. (medlineplus.gov)
  • Having the HLA-B27 protein doesn't mean you have AS-and vice versa. (spineuniverse.com)
  • This is due to the high capacity of virus-specific, HLA-B27-restricted CD8+ T cells for both intrinsic (i.e. polyfunctionality, high avidity, low sensitivity to Treg cell-mediated suppression) and extrinsic (i.e. rapid and efficient antigen processing and presentation) factors. (archives-ouvertes.fr)
  • 2005). "Nomenclature for factors of the HLA system, 2004" . (wikipedia.org)
  • The association with HLA-B27 remains one of the strongest known links between these entities and the major histocompatibility complex. (scielo.org.co)
  • Antigen presentation in Salmonella-infected cells could be modulated by changes in the composition of the proteasome, which is the major proteolytic system that generates major histocompatibility complex class I ligands. (csic.es)
  • A normal test result for a patient is a negative result that says no HLA-B27 is present. (reference.com)
  • The results indicate that these clones display dual recognition for HLA-B27.1 and for HLA-DR2 and suggest that HLA-B27.1 may share at least one epitope that is closely related to some stimulatory Dw determinants present on the HLA-DR2 antigens. (rupress.org)
  • For example, HLA B27 is present in 80% of patients with Reiter syndrome. (wbccount.org)
  • HLA-B27 is an antigen that is present in about 8 percent of normal, healthy people. (chop.edu)