Antigens determined by leukocyte loci found on chromosome 6, the major histocompatibility loci in humans. They are polypeptides or glycoproteins found on most nucleated cells and platelets, determine tissue types for transplantation, and are associated with certain diseases.
Substances that are recognized by the immune system and induce an immune reaction.
Class I human histocompatibility (HLA) surface antigens encoded by more than 30 detectable alleles on locus B of the HLA complex, the most polymorphic of all the HLA specificities. Several of these antigens (e.g., HLA-B27, -B7, -B8) are strongly associated with predisposition to rheumatoid and other autoimmune disorders. Like other class I HLA determinants, they are involved in the cellular immune reactivity of cytolytic T lymphocytes.
A group of antigens that includes both the major and minor histocompatibility antigens. The former are genetically determined by the major histocompatibility complex. They determine tissue type for transplantation and cause allograft rejections. The latter are systems of allelic alloantigens that can cause weak transplant rejection.
A subclass of HLA-D antigens that consist of alpha and beta chains. The inheritance of HLA-DR antigens differs from that of the HLA-DQ ANTIGENS and HLA-DP ANTIGENS.
Antigens on surfaces of cells, including infectious or foreign cells or viruses. They are usually protein-containing groups on cell membranes or walls and may be isolated.
Proteins, glycoprotein, or lipoprotein moieties on surfaces of tumor cells that are usually identified by monoclonal antibodies. Many of these are of either embryonic or viral origin.
Substances elaborated by bacteria that have antigenic activity.
Polymorphic class I human histocompatibility (HLA) surface antigens present on almost all nucleated cells. At least 20 antigens have been identified which are encoded by the A locus of multiple alleles on chromosome 6. They serve as targets for T-cell cytolytic responses and are involved with acceptance or rejection of tissue/organ grafts.
Identification of the major histocompatibility antigens of transplant DONORS and potential recipients, usually by serological tests. Donor and recipient pairs should be of identical ABO blood group, and in addition should be matched as closely as possible for HISTOCOMPATIBILITY ANTIGENS in order to minimize the likelihood of allograft rejection. (King, Dictionary of Genetics, 4th ed)
Human immune-response or Class II antigens found mainly, but not exclusively, on B-lymphocytes and produced from genes of the HLA-D locus. They are extremely polymorphic families of glycopeptides, each consisting of two chains, alpha and beta. This group of antigens includes the -DR, -DQ and -DP designations, of which HLA-DR is most studied; some of these glycoproteins are associated with certain diseases, possibly of immune etiology.
A specific HLA-B surface antigen subtype. Members of this subtype contain alpha chains that are encoded by the HLA-B*07 allele family.
Substances elaborated by viruses that have antigenic activity.
An HLA-DR antigen which is associated with HLA-DRB1 CHAINS encoded by DRB1*03 alleles.
Large, transmembrane, non-covalently linked glycoproteins (alpha and beta). Both chains can be polymorphic although there is more structural variation in the beta chains. The class II antigens in humans are called HLA-D ANTIGENS and are coded by a gene on chromosome 6. In mice, two genes named IA and IE on chromosome 17 code for the H-2 antigens. The antigens are found on B-lymphocytes, macrophages, epidermal cells, and sperm and are thought to mediate the competence of and cellular cooperation in the immune response. The term IA antigens used to refer only to the proteins encoded by the IA genes in the mouse, but is now used as a generic term for any class II histocompatibility antigen.
Membrane glycoproteins consisting of an alpha subunit and a BETA 2-MICROGLOBULIN beta subunit. In humans, highly polymorphic genes on CHROMOSOME 6 encode the alpha subunits of class I antigens and play an important role in determining the serological specificity of the surface antigen. Class I antigens are found on most nucleated cells and are generally detected by their reactivity with alloantisera. These antigens are recognized during GRAFT REJECTION and restrict cell-mediated lysis of virus-infected cells.
A specific HLA-B surface antigen subtype. Members of this subtype contain alpha chains that are encoded by the HLA-B*35 allele family.
DNA probes specific for the human leukocyte antigen genes, which represent the major histocompatibility determinants in humans. The four known loci are designated as A, B, C, and D. Specific antigens are identified by a locus notation and number, e.g., HLA-A11. The inheritance of certain HLA alleles is associated with increased risk for certain diseases (e.g., insulin-dependent diabetes mellitus).
Serum proteins with an electrophoretic mobility that falls between ALPHA-GLOBULINS and GAMMA-GLOBULINS.
A specific HLA-B surface antigen subtype. Members of this subtype contain alpha chains that are encoded by the HLA-B*08 allele family.
Antibodies from an individual that react with ISOANTIGENS of another individual of the same species.
An HLA-DR antigen which is associated with HLA-DRB1 CHAINS encoded by DRB1*04 alleles.
Class I human histocompatibility (HLA) antigens encoded by a small cluster of structural genes at the C locus on chromosome 6. They have significantly lower immunogenicity than the HLA-A and -B determinants and are therefore of minor importance in donor/recipient crossmatching. Their primary role is their high-risk association with certain disease manifestations (e.g., spondylarthritis, psoriasis, multiple myeloma).
A group of the D-related HLA antigens found to differ from the DR antigens in genetic locus and therefore inheritance. These antigens are polymorphic glycoproteins comprising alpha and beta chains and are found on lymphoid and other cells, often associated with certain diseases.
Sites on an antigen that interact with specific antibodies.
An 11-kDa protein associated with the outer membrane of many cells including lymphocytes. It is the small subunit of the MHC class I molecule. Association with beta 2-microglobulin is generally required for the transport of class I heavy chains from the endoplasmic reticulum to the cell surface. Beta 2-microglobulin is present in small amounts in serum, csf, and urine of normal people, and to a much greater degree in the urine and plasma of patients with tubular proteinemia, renal failure, or kidney transplants.
HLA-DR antigen subtypes that have been classified according to their affinity to specific ANTIBODIES. The DNA sequence analyses of HLA-DR ALPHA-CHAINS and HLA-DR BETA-CHAINS has for the most part revealed the specific alleles that are responsible for each serological subtype.
Any part or derivative of any protozoan that elicits immunity; malaria (Plasmodium) and trypanosome antigens are presently the most frequently encountered.
A specific HLA-B surface antigen subtype. Members of this subtype contain alpha chains that are encoded by the HLA-B*27 allele family.
Antibodies produced by a single clone of cells.
Polyomavirus antigens which cause infection and cellular transformation. The large T antigen is necessary for the initiation of viral DNA synthesis, repression of transcription of the early region and is responsible in conjunction with the middle T antigen for the transformation of primary cells. Small T antigen is necessary for the completion of the productive infection cycle.
A broad specificity HLA-DR antigen that is associated with HLA-DRB1 CHAINS encoded by DRB1*01:15 and DRB1*01:16 alleles.
A specific HLA-A surface antigen subtype. Members of this subtype contain alpha chains that are encoded by the HLA-A*03 allele family.
Substances of fungal origin that have antigenic activity.
Differentiation antigens residing on mammalian leukocytes. CD stands for cluster of differentiation, which refers to groups of monoclonal antibodies that show similar reactivity with certain subpopulations of antigens of a particular lineage or differentiation stage. The subpopulations of antigens are also known by the same CD designation.
Any part or derivative of a helminth that elicits an immune reaction. The most commonly seen helminth antigens are those of the schistosomes.
The major group of transplantation antigens in the mouse.
Inflammation of the anterior uvea comprising the iris, angle structures, and the ciliary body. Manifestations of this disorder include ciliary injection, exudation into the anterior chamber, iris changes, and adhesions between the iris and lens (posterior synechiae). Intraocular pressure may be increased or reduced.
Lymphocytes responsible for cell-mediated immunity. Two types have been identified - cytotoxic (T-LYMPHOCYTES, CYTOTOXIC) and helper T-lymphocytes (T-LYMPHOCYTES, HELPER-INDUCER). They are formed when lymphocytes circulate through the THYMUS GLAND and differentiate to thymocytes. When exposed to an antigen, they divide rapidly and produce large numbers of new T cells sensitized to that antigen.
Organic compounds that contain GOLD as an integral part of the molecule. Some are used as ANTIRHEUMATIC AGENTS. The term chrysotherapy derives from an ancient Greek term for gold.
An anti-gas warfare agent that is effective against Lewisite (dichloro(2-chlorovinyl)arsine) and formerly known as British Anti-Lewisite or BAL. It acts as a chelating agent and is used in the treatment of arsenic, gold, and other heavy metal poisoning.
The genetic region which contains the loci of genes which determine the structure of the serologically defined (SD) and lymphocyte-defined (LD) TRANSPLANTATION ANTIGENS, genes which control the structure of the IMMUNE RESPONSE-ASSOCIATED ANTIGENS, HUMAN; the IMMUNE RESPONSE GENES which control the ability of an animal to respond immunologically to antigenic stimuli, and genes which determine the structure and/or level of the first four components of complement.
Genetic loci in the vertebrate major histocompatibility complex which encode polymorphic characteristics not related to immune responsiveness or complement activity, e.g., B loci (chicken), DLA (dog), GPLA (guinea pig), H-2 (mouse), RT-1 (rat), HLA-A, -B, and -C class I genes of man.
Genetic loci in the vertebrate major histocompatibility complex that encode polymorphic products which control the immune response to specific antigens. The genes are found in the HLA-D region in humans and in the I region in mice.
A glycoprotein that is secreted into the luminal surface of the epithelia in the gastrointestinal tract. It is found in the feces and pancreaticobiliary secretions and is used to monitor the response to colon cancer treatment.
The degree of antigenic similarity between the tissues of different individuals, which determines the acceptance or rejection of allografts.
Established cell cultures that have the potential to propagate indefinitely.
Those proteins recognized by antibodies from serum of animals bearing tumors induced by viruses; these proteins are presumably coded for by the nucleic acids of the same viruses that caused the neoplastic transformation.
The property of antibodies which enables them to react with some ANTIGENIC DETERMINANTS and not with others. Specificity is dependent on chemical composition, physical forces, and molecular structure at the binding site.
Molecules on the surface of T-lymphocytes that recognize and combine with antigens. The receptors are non-covalently associated with a complex of several polypeptides collectively called CD3 antigens (ANTIGENS, CD3). Recognition of foreign antigen and the major histocompatibility complex is accomplished by a single heterodimeric antigen-receptor structure, composed of either alpha-beta (RECEPTORS, ANTIGEN, T-CELL, ALPHA-BETA) or gamma-delta (RECEPTORS, ANTIGEN, T-CELL, GAMMA-DELTA) chains.
A specific HLA-A surface antigen subtype. Members of this subtype contain alpha chains that are encoded by the HLA-A*02 allele family.
Variant forms of the same gene, occupying the same locus on homologous CHROMOSOMES, and governing the variants in production of the same gene product.
The ability of tumors to evade destruction by the IMMUNE SYSTEM. Theories concerning possible mechanisms by which this takes place involve both cellular immunity (IMMUNITY, CELLULAR) and humoral immunity (ANTIBODY FORMATION), and also costimulatory pathways related to CD28 antigens (ANTIGENS, CD28) and CD80 antigens (ANTIGENS, CD80).
The phenomenon of target cell destruction by immunologically active effector cells. It may be brought about directly by sensitized T-lymphocytes or by lymphoid or myeloid "killer" cells, or it may be mediated by cytotoxic antibody, cytotoxic factor released by lymphoid cells, or complement.
Immunized T-lymphocytes which can directly destroy appropriate target cells. These cytotoxic lymphocytes may be generated in vitro in mixed lymphocyte cultures (MLC), in vivo during a graft-versus-host (GVH) reaction, or after immunization with an allograft, tumor cell or virally transformed or chemically modified target cell. The lytic phenomenon is sometimes referred to as cell-mediated lympholysis (CML). These CD8-positive cells are distinct from NATURAL KILLER CELLS and NATURAL KILLER T-CELLS. There are two effector phenotypes: TC1 and TC2.
Antibodies that react with self-antigens (AUTOANTIGENS) of the organism that produced them.
Nuclear antigen with a role in DNA synthesis, DNA repair, and cell cycle progression. PCNA is required for the coordinated synthesis of both leading and lagging strands at the replication fork during DNA replication. PCNA expression correlates with the proliferation activity of several malignant and non-malignant cell types.
The genetic constitution of individuals with respect to one member of a pair of allelic genes, or sets of genes that are closely linked and tend to be inherited together such as those of the MAJOR HISTOCOMPATIBILITY COMPLEX.
Lymphoid cells concerned with humoral immunity. They are short-lived cells resembling bursa-derived lymphocytes of birds in their production of immunoglobulin upon appropriate stimulation.
White blood cells formed in the body's lymphoid tissue. The nucleus is round or ovoid with coarse, irregularly clumped chromatin while the cytoplasm is typically pale blue with azurophilic (if any) granules. Most lymphocytes can be classified as either T or B (with subpopulations of each), or NATURAL KILLER CELLS.
IMMUNOGLOBULINS on the surface of B-LYMPHOCYTES. Their MESSENGER RNA contains an EXON with a membrane spanning sequence, producing immunoglobulins in the form of type I transmembrane proteins as opposed to secreted immunoglobulins (ANTIBODIES) which do not contain the membrane spanning segment.
A glycoprotein that is a kallikrein-like serine proteinase and an esterase, produced by epithelial cells of both normal and malignant prostate tissue. It is an important marker for the diagnosis of prostate cancer.
The lipopolysaccharide-protein somatic antigens, usually from gram-negative bacteria, important in the serological classification of enteric bacilli. The O-specific chains determine the specificity of the O antigens of a given serotype. O antigens are the immunodominant part of the lipopolysaccharide molecule in the intact bacterial cell. (From Singleton & Sainsbury, Dictionary of Microbiology and Molecular Biology, 2d ed)
A constitution or condition of the body which makes the tissues react in special ways to certain extrinsic stimuli and thus tends to make the individual more than usually susceptible to certain diseases.
The proportion of one particular in the total of all ALLELES for one genetic locus in a breeding POPULATION.
The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.
Immunoglobulin molecules having a specific amino acid sequence by virtue of which they interact only with the ANTIGEN (or a very similar shape) that induced their synthesis in cells of the lymphoid series (especially PLASMA CELLS).
Differentiation antigens found on thymocytes and on cytotoxic and suppressor T-lymphocytes. CD8 antigens are members of the immunoglobulin supergene family and are associative recognition elements in MHC (Major Histocompatibility Complex) Class I-restricted interactions.
A trisaccharide antigen expressed on glycolipids and many cell-surface glycoproteins. In the blood the antigen is found on the surface of NEUTROPHILS; EOSINOPHILS; and MONOCYTES. In addition, CD15 antigen is a stage-specific embryonic antigen.
A disorder of neuromuscular transmission characterized by weakness of cranial and skeletal muscles. Autoantibodies directed against acetylcholine receptors damage the motor endplate portion of the NEUROMUSCULAR JUNCTION, impairing the transmission of impulses to skeletal muscles. Clinical manifestations may include diplopia, ptosis, and weakness of facial, bulbar, respiratory, and proximal limb muscles. The disease may remain limited to the ocular muscles. THYMOMA is commonly associated with this condition. (Adams et al., Principles of Neurology, 6th ed, p1459)
A malignant neoplasm derived from cells that are capable of forming melanin, which may occur in the skin of any part of the body, in the eye, or, rarely, in the mucous membranes of the genitalia, anus, oral cavity, or other sites. It occurs mostly in adults and may originate de novo or from a pigmented nevus or malignant lentigo. Melanomas frequently metastasize widely, and the regional lymph nodes, liver, lungs, and brain are likely to be involved. The incidence of malignant skin melanomas is rising rapidly in all parts of the world. (Stedman, 25th ed; from Rook et al., Textbook of Dermatology, 4th ed, p2445)
Carbohydrate antigens expressed by malignant tissue. They are useful as tumor markers and are measured in the serum by means of a radioimmunoassay employing monoclonal antibodies.
An immunoassay utilizing an antibody labeled with an enzyme marker such as horseradish peroxidase. While either the enzyme or the antibody is bound to an immunosorbent substrate, they both retain their biologic activity; the change in enzyme activity as a result of the enzyme-antibody-antigen reaction is proportional to the concentration of the antigen and can be measured spectrophotometrically or with the naked eye. Many variations of the method have been developed.
Antigens that exist in alternative (allelic) forms in a single species. When an isoantigen is encountered by species members who lack it, an immune response is induced. Typical isoantigens are the BLOOD GROUP ANTIGENS.
The transference of a kidney from one human or animal to another.
Serological reactions in which an antiserum against one antigen reacts with a non-identical but closely related antigen.
The specific failure of a normally responsive individual to make an immune response to a known antigen. It results from previous contact with the antigen by an immunologically immature individual (fetus or neonate) or by an adult exposed to extreme high-dose or low-dose antigen, or by exposure to radiation, antimetabolites, antilymphocytic serum, etc.
The major immunoglobulin isotype class in normal human serum. There are several isotype subclasses of IgG, for example, IgG1, IgG2A, and IgG2B.
The major interferon produced by mitogenically or antigenically stimulated LYMPHOCYTES. It is structurally different from TYPE I INTERFERON and its major activity is immunoregulation. It has been implicated in the expression of CLASS II HISTOCOMPATIBILITY ANTIGENS in cells that do not normally produce them, leading to AUTOIMMUNE DISEASES.
The survival of a graft in a host, the factors responsible for the survival and the changes occurring within the graft during growth in the host.
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
Individuals supplying living tissue, organs, cells, blood or blood components for transfer or transplantation to histocompatible recipients.
Complex of at least five membrane-bound polypeptides in mature T-lymphocytes that are non-covalently associated with one another and with the T-cell receptor (RECEPTORS, ANTIGEN, T-CELL). The CD3 complex includes the gamma, delta, epsilon, zeta, and eta chains (subunits). When antigen binds to the T-cell receptor, the CD3 complex transduces the activating signals to the cytoplasm of the T-cell. The CD3 gamma and delta chains (subunits) are separate from and not related to the gamma/delta chains of the T-cell receptor (RECEPTORS, ANTIGEN, T-CELL, GAMMA-DELTA).
Those hepatitis B antigens found on the surface of the Dane particle and on the 20 nm spherical and tubular particles. Several subspecificities of the surface antigen are known. These were formerly called the Australia antigen.
Sets of cell surface antigens located on BLOOD CELLS. They are usually membrane GLYCOPROTEINS or GLYCOLIPIDS that are antigenically distinguished by their carbohydrate moieties.
The outward appearance of the individual. It is the product of interactions between genes, and between the GENOTYPE and the environment.
Morphologic alteration of small B LYMPHOCYTES or T LYMPHOCYTES in culture into large blast-like cells able to synthesize DNA and RNA and to divide mitotically. It is induced by INTERLEUKINS; MITOGENS such as PHYTOHEMAGGLUTININS, and by specific ANTIGENS. It may also occur in vivo as in GRAFT REJECTION.
Multi-subunit proteins which function in IMMUNITY. They are produced by B LYMPHOCYTES from the IMMUNOGLOBULIN GENES. They are comprised of two heavy (IMMUNOGLOBULIN HEAVY CHAINS) and two light chains (IMMUNOGLOBULIN LIGHT CHAINS) with additional ancillary polypeptide chains depending on their isoforms. The variety of isoforms include monomeric or polymeric forms, and transmembrane forms (B-CELL ANTIGEN RECEPTORS) or secreted forms (ANTIBODIES). They are divided by the amino acid sequence of their heavy chains into five classes (IMMUNOGLOBULIN A; IMMUNOGLOBULIN D; IMMUNOGLOBULIN E; IMMUNOGLOBULIN G; IMMUNOGLOBULIN M) and various subclasses.
Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others.
An immune response with both cellular and humoral components, directed against an allogeneic transplant, whose tissue antigens are not compatible with those of the recipient.
The sum of the weight of all the atoms in a molecule.
55-kDa antigens found on HELPER-INDUCER T-LYMPHOCYTES and on a variety of other immune cell types. CD4 antigens are members of the immunoglobulin supergene family and are implicated as associative recognition elements in MAJOR HISTOCOMPATIBILITY COMPLEX class II-restricted immune responses. On T-lymphocytes they define the helper/inducer subset. CD4 antigens also serve as INTERLEUKIN-15 receptors and bind to the HIV receptors, binding directly to the HIV ENVELOPE PROTEIN GP120.
High-molecular weight glycoproteins uniquely expressed on the surface of LEUKOCYTES and their hemopoietic progenitors. They contain a cytoplasmic protein tyrosine phosphatase activity which plays a role in intracellular signaling from the CELL SURFACE RECEPTORS. The CD45 antigens occur as multiple isoforms that result from alternative mRNA splicing and differential usage of three exons.
Molecules on the surface of B- and T-lymphocytes that recognize and combine with specific antigens.
Antigens of the virion of the HEPATITIS B VIRUS or the Dane particle, its surface (HEPATITIS B SURFACE ANTIGENS), core (HEPATITIS B CORE ANTIGENS), and other associated antigens, including the HEPATITIS B E ANTIGENS.
Transmembrane proteins that form the beta subunits of the HLA-DQ antigens.
Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
The processes triggered by interactions of ANTIBODIES with their ANTIGENS.
A subtype of HLA-DRB beta chains that includes over one hundred allele variants. The HLA-DRB1 subtype is associated with several of the HLA-DR SEROLOGICAL SUBTYPES.
The production of ANTIBODIES by proliferating and differentiated B-LYMPHOCYTES under stimulation by ANTIGENS.
Glycoproteins expressed on cortical thymocytes and on some dendritic cells and B-cells. Their structure is similar to that of MHC Class I and their function has been postulated as similar also. CD1 antigens are highly specific markers for human LANGERHANS CELLS.
The introduction of whole blood or blood component directly into the blood stream. (Dorland, 27th ed)
Serum that contains antibodies. It is obtained from an animal that has been immunized either by ANTIGEN injection or infection with microorganisms containing the antigen.
Immunoglobulins produced in a response to BACTERIAL ANTIGENS.
Deliberate stimulation of the host's immune response. ACTIVE IMMUNIZATION involves administration of ANTIGENS or IMMUNOLOGIC ADJUVANTS. PASSIVE IMMUNIZATION involves administration of IMMUNE SERA or LYMPHOCYTES or their extracts (e.g., transfer factor, immune RNA) or transplantation of immunocompetent cell producing tissue (thymus or bone marrow).
Transplantation between individuals of the same species. Usually refers to genetically disparate individuals in contradistinction to isogeneic transplantation for genetically identical individuals.
Antigens expressed primarily on the membranes of living cells during sequential stages of maturation and differentiation. As immunologic markers they have high organ and tissue specificity and are useful as probes in studies of normal cell development as well as neoplastic transformation.
A chronic systemic disease, primarily of the joints, marked by inflammatory changes in the synovial membranes and articular structures, widespread fibrinoid degeneration of the collagen fibers in mesenchymal tissues, and by atrophy and rarefaction of bony structures. Etiology is unknown, but autoimmune mechanisms have been implicated.
A subtype of DIABETES MELLITUS that is characterized by INSULIN deficiency. It is manifested by the sudden onset of severe HYPERGLYCEMIA, rapid progression to DIABETIC KETOACIDOSIS, and DEATH unless treated with insulin. The disease may occur at any age, but is most common in childhood or adolescence.
Antigens associated with specific proteins of the human adult T-cell immunodeficiency virus (HIV); also called HTLV-III-associated and lymphadenopathy-associated virus (LAV) antigens.
A specific HLA-A surface antigen subtype. Members of this subtype contain alpha chains that are encoded by the HLA-A*01 allele family.
A melanosome-specific protein that plays a role in the expression, stability, trafficking, and processing of GP100 MELANOMA ANTIGEN, which is critical to the formation of Stage II MELANOSOMES. The protein is used as an antigen marker for MELANOMA cells.
A costimulatory ligand expressed by ANTIGEN-PRESENTING CELLS that binds to CTLA-4 ANTIGEN with high specificity and to CD28 ANTIGEN with low specificity. The interaction of CD80 with CD28 ANTIGEN provides a costimulatory signal to T-LYMPHOCYTES, while its interaction with CTLA-4 ANTIGEN may play a role in inducing PERIPHERAL TOLERANCE.
The ability of a substance to be dissolved, i.e. to form a solution with another substance. (From McGraw-Hill Dictionary of Scientific and Technical Terms, 6th ed)
Immunologic techniques based on the use of: (1) enzyme-antibody conjugates; (2) enzyme-antigen conjugates; (3) antienzyme antibody followed by its homologous enzyme; or (4) enzyme-antienzyme complexes. These are used histologically for visualizing or labeling tissue specimens.
Nuclear antigens encoded by VIRAL GENES found in HUMAN HERPESVIRUS 4. At least six nuclear antigens have been identified.
Technique using an instrument system for making, processing, and displaying one or more measurements on individual cells obtained from a cell suspension. Cells are usually stained with one or more fluorescent dyes specific to cell components of interest, e.g., DNA, and fluorescence of each cell is measured as it rapidly transverses the excitation beam (laser or mercury arc lamp). Fluorescence provides a quantitative measure of various biochemical and biophysical properties of the cell, as well as a basis for cell sorting. Other measurable optical parameters include light absorption and light scattering, the latter being applicable to the measurement of cell size, shape, density, granularity, and stain uptake.
Endogenous tissue constituents that have the ability to interact with AUTOANTIBODIES and cause an immune response.
A specific HLA-B surface antigen subtype. Members of this subtype contain alpha chains that are encoded by the HLA-B*44 allele family.
Antigens stimulating the formation of, or combining with heterophile antibodies. They are cross-reacting antigens found in phylogenetically unrelated species.
Differentiation antigens expressed on B-lymphocytes and B-cell precursors. They are involved in regulation of B-cell proliferation.
The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.
A heterogeneous group of immunocompetent cells that mediate the cellular immune response by processing and presenting antigens to the T-cells. Traditional antigen-presenting cells include MACROPHAGES; DENDRITIC CELLS; LANGERHANS CELLS; and B-LYMPHOCYTES. FOLLICULAR DENDRITIC CELLS are not traditional antigen-presenting cells, but because they hold antigen on their cell surface in the form of IMMUNE COMPLEXES for B-cell recognition they are considered so by some authors.
A critical subpopulation of regulatory T-lymphocytes involved in MHC Class I-restricted interactions. They include both cytotoxic T-lymphocytes (T-LYMPHOCYTES, CYTOTOXIC) and CD8+ suppressor T-lymphocytes.

Coeliac disease and dermatitis herpetiformis: further studies of their relationship. (1/3960)

Using diagnostic criteria which are currently accepted as most reliable we have found that 19% (9/47) of patients with dermatitis herpetiformis (DH) have no evidence of coeliac disease. The incidence of HL-A8 in the DH patients was 78%, which is considerably greater than that in healthy controls and no different from that reported in coeliac disease. Furthermore, the incidence of HL-A8 was just as much increased in those DH patients without evidence of coeliac disease suggesting that HL-A8 is associated with DH per se--that is, regardless of its association with coeliac disease.  (+info)

Histocompatibility antigens in inflammatory bowel disease. Their clinical significance and their association with arthropathy with special reference to HLA-B27 (W27). (2/3960)

Histocompatibility (HLA) antigen phenotypes have been studied in 100 patients with ulcerative colitis, 100 with Crohn's disease, and 283 normal controls. In addition the incidence of ankylosing spondylitis, sacroiliitis, and "enteropathic" peripheral arthropathy was determined in the patients with inflammatory bowel disease (IBD). There was no significant difference in antigen frequency between patients and controls. However, the incidence of HLA-B27 was increased in the patients complicated by ankylosing spondylitis and/or sacroiliitis in both ulcerative colitis and Crohn's disease. In contrast, none of the 29 IBD patients with "enteropathic" peripheral arthropathy had B27 antigen. Furthermore, ankylosing spondylitis was found more frequently in ulcerative colitis bearing HLA-B27 compared with non-B27 patients (P less than 0-01). The same was found in Crohn's disease, although this difference was not statistically significant. In addition, 12 of 14 ulcerative colitis patients and five out of six Crohn's patients with HLA-B27 had total colitis, compared with the frequency of total colitis in non-B27 patients (P less than 0-024 and less than 0-03 respectively). The data suggest that B27 histocompatibility antigen could be a pathogenetic discriminator between the arthropathies in IBD and may be of prognostic significance with respect to extension and severity of the disease.  (+info)

Structure of CD94 reveals a novel C-type lectin fold: implications for the NK cell-associated CD94/NKG2 receptors. (3/3960)

The crystal structure of the extracellular domain of CD94, a component of the CD94/NKG2 NK cell receptor, has been determined to 2.6 A resolution, revealing a unique variation of the C-type lectin fold. In this variation, the second alpha helix, corresponding to residues 102-112, is replaced by a loop, the putative carbohydrate-binding site is significantly altered, and the Ca2+-binding site appears nonfunctional. This structure may serve as a prototype for other NK cell receptors such as Ly-49, NKR-P1, and CD69. The CD94 dimer observed in the crystal has an extensive hydrophobic interface that stabilizes the loop conformation of residues 102-112. The formation of this dimer reveals a putative ligand-binding region for HLA-E and suggests how NKG2 interacts with CD94.  (+info)

Human uterine lymphocytes. (4/3960)

During the luteal phase and the early months of pregnancy, there is a dense mucosal infiltration of CD56+ natural killer (NK) cells. These uterine NK cells have a phenotype (CD56bright, CD16-, mCD3-) which distinguishes them from peripheral blood NK cells (CD56dim, CD16bright, mCD3-). The uterine NK cells are in close association with extravillous trophoblast (EVT) cells which infiltrate into the decidua and maternal spiral arteries. This subpopulation of trophoblast expresses two human leukocyte antigen (HLA) class I molecules, HLA-G and HLA-C. Circulating NK cells express receptors for HLA class I molecules. We have recently found evidence that similar receptors are present on decidual NK cells belonging to both the Killer Inhibitory Receptor (KIR) and CD94 families. The repertoire of NK receptors expressed varies between different women. The findings indicate that decidual NK cells do have receptors for trophoblast HLA class I molecules. Experiments are underway to determine the effects of this interaction on NK cell function.  (+info)

Mutation screening in British 21-hydroxylase deficiency families and development of novel microsatellite based approaches to prenatal diagnosis. (5/3960)

21-hydroxylase deficiency is a recessively inherited disorder of steroidogenesis, resulting from mutations in the CYP21 gene. This 3.5 kb gene and a highly related CYP21P pseudogene reside on tandemly duplicated 30 kb segments of DNA in the class III HLA region, and the great majority of pathogenic mutations result from sequence exchanges involving the duplicated units. We now describe a comprehensive survey of CYP21 mutations in the British population, encompassing a screen for 17 different mutations in a total of 284 disease chromosomes. The most common mutations were as follows: large scale deletions/conversions (45% of the affected chromosomes), the intron 2 splice mutation (30.3%), R357W (9.8%), and I172N (7.0%). Mutations were detected in over 92% of the chromosomes examined, suggesting that accurate DNA based diagnosis is possible in most cases using the described strategy. In order to extend highly accurate prenatal diagnosis to all families where samples are available from a previously affected child, we have developed a linkage analysis approach using novel, highly informative microsatellite markers from the class III HLA region.  (+info)

The predisposition to type 1 diabetes linked to the human leukocyte antigen complex includes at least one non-class II gene. (6/3960)

The human leukocyte antigen (HLA) complex, encompassing 3.5 Mb of DNA from the centromeric HLA-DPB2 locus to the telomeric HLA-F locus on chromosome 6p21, encodes a major part of the genetic predisposition to develop type 1 diabetes, designated "IDDM1." A primary role for allelic variation of the class II HLA-DRB1, HLA-DQA1, and HLA-DQB1 loci has been established. However, studies of animals and humans have indicated that other, unmapped, major histocompatibility complex (MHC)-linked genes are participating in IDDM1. The strong linkage disequilibrium between genes in this complex makes mapping a difficult task. In the present paper, we report on the approach we have devised to circumvent the confounding effects of disequilibrium between class II alleles and alleles at other MHC loci. We have scanned 12 Mb of the MHC and flanking chromosome regions with microsatellite polymorphisms and analyzed the transmission of these marker alleles to diabetic probands from parents who were homozygous for the alleles of the HLA-DRB1, HLA-DQA1, and HLA-DQB1 genes. Our analysis, using three independent family sets, suggests the presence of an additional type I diabetes gene (or genes). This approach is useful for the analysis of other loci linked to common diseases, to verify if a candidate polymorphism can explain all of the association of a region or if the association is due to two or more loci in linkage disequilibrium with each other.  (+info)

Long-term fetal microchimerism in peripheral blood mononuclear cell subsets in healthy women and women with scleroderma. (7/3960)

Fetal CD34(+) CD38(+) cells have recently been found to persist in maternal peripheral blood for many years after pregnancy. CD34(+) CD38(+) cells are progenitor cells that can differentiate into mature immune-competent cells. We asked whether long-term fetal microchimerism occurs in T lymphocyte, B lymphocyte, monocyte, and natural-killer cell populations of previously pregnant women. We targeted women with sons and used polymerase chain reaction for a Y-chromosome-specific sequence to test DNA extracted from peripheral blood mononuclear cells (PBMC) and from CD3, CD19, CD14, and CD56/16 sorted subsets. We also asked whether persistent microchimerism might contribute to subsequent autoimmune disease in the mother and included women with the autoimmune disease scleroderma. Scleroderma has a peak incidence in women after childbearing years and has clinical similarities to chronic graft-versus-host disease that occurs after allogeneic hematopoietic stem-cell transplantation, known to involve chimerism. Sixty-eight parous women were studied for male DNA in PBMC and 20 for PBMC subsets. Microchimerism was found in PBMC from 33% (16 of 48) of healthy women and 60% (12 of 20) women with scleroderma, P =.046. Microchimerism was found in some women in CD3, CD19, CD14, and CD56/16 subsets including up to 38 years after pregnancy. Microchimerism in PBMC subsets was not appreciably more frequent in scleroderma patients than in healthy controls. Overall, microchimerism was found in CD3, CD19, and CD14 subsets in approximately one third of women and in CD56/16 in one half of women. HLA typing of mothers and sons indicated that HLA compatibility was not a requirement for persistent microchimerism in PBMC subsets. Fetal microchimerism in the face of HLA disparity implies that specific maternal immunoregulatory pathways exist that permit persistence but prevent effector function of these cells in normal women. Although microchimerism in PBMC was more frequent in women with scleroderma than healthy controls additional studies will be necessary to determine whether microchimerism plays a role in the pathogenesis of this or other autoimmune diseases.  (+info)

HLA and HIV-1: heterozygote advantage and B*35-Cw*04 disadvantage. (8/3960)

A selective advantage against infectious disease associated with increased heterozygosity at the human major histocompatibility complex [human leukocyte antigen (HLA) class I and class II] is believed to play a major role in maintaining the extraordinary allelic diversity of these genes. Maximum HLA heterozygosity of class I loci (A, B, and C) delayed acquired immunodeficiency syndrome (AIDS) onset among patients infected with human immunodeficiency virus-type 1 (HIV-1), whereas individuals who were homozygous for one or more loci progressed rapidly to AIDS and death. The HLA class I alleles B*35 and Cw*04 were consistently associated with rapid development of AIDS-defining conditions in Caucasians. The extended survival of 28 to 40 percent of HIV-1-infected Caucasian patients who avoided AIDS for ten or more years can be attributed to their being fully heterozygous at HLA class I loci, to their lacking the AIDS-associated alleles B*35 and Cw*04, or to both.  (+info)

Shop RT1 class I histocompatibility antigen ELISA Kit, Recombinant Protein and RT1 class I histocompatibility antigen Antibody at MyBioSource. Custom ELISA Kit, Recombinant Protein and Antibody are available.
Human leukocyte antigen-E (HLA-E) normally presents an HLA class Ia signal peptide to the NKG2A/C-CD94 regulatory receptors on natural killer (NK) cells and T cell subsets. Rhesus macaques immunized with a cytomegalovirus-vectored simian immunodeficiency virus (SIV) vaccine generated Mamu-E (HLA-E homolog)-restricted T cell responses that mediated post-challenge SIV replication arrest in |50% of animals. However, HIV-1-specific, HLA-E-restricted T cells have not been observed in HIV-1-infected individuals. Here, HLA-E-restricted, HIV-1-specific CD8 + T cells were primed in vitro. These T cell clones and allogeneic CD8 + T cells transduced with their T cell receptors suppressed HIV-1 replication in CD4 + T cells in vitro. Vaccine induction of efficacious HLA-E-restricted HIV-1-specific T cells should therefore be possible.
Transplantation improves the health and quality of life for patients suffering from renal failure, but for many, antibodies specific for HLA antigens create a substantial barrier. Our center has developed a desensitization protocol to remove HLA-specific antibodies and successfully transplant these sensitized patients. This protocol involves alternate day plasmapheresis, intravenous immunoglobulin (IvIg), and immunosuppressants. We use a Luminex assay to detect HLA-specific antibody and developed a flow cytometric technique using HLA tetramer molecules to quantitate HLA-specific B cells. Following desensitization and transplantation, we observe a sustained loss of donor-specific HLA antibodies, while antibodies specific for 3rd party HLA return to pre-treatment levels. Interestingly, B cells specific for donor-specific HLA persist. We hypothesize that transplantation in the absence of inflammation, due to continued desensitization treatment, allows for the induction of B cell anergy toward ...
HLA class I histocompatibility antigen, alpha chain F is a protein that in humans is encoded by the HLA-F gene. The Major Histocompatibility Complex (MHC) is a group of cell surface proteins that in humans is also called the Human Leukocyte Antigen (HLA) complex. These proteins are encoded by a cluster of genes known as the HLA locus. The HLA locus occupies a ~ 3Mbp stretch that is located on the short arm of chromosome 6, specifically on 6p21.1-21.3. The MHC proteins are classified into three main categories, namely class I, II, and III. There are over 140 genes within the HLA locus and they are often called HLA genes. HLA-A, B, and C are the classical class I genes and HLA-E, F and G are the nonclassical class I genes. The protein encoded from the gene HLA-F was originally isolated from the human lymphoblastoid cell line 721. The HLA-F gene is located on the short arm of chromosome 6, telomeric to the HLA-A locus. HLA-F has little allelic polymorphism and is highly conserved in other primates. ...
Although association between CMV infection and allograft rejection is well admitted, the precise mechanisms involved remain uncertain. Here, we report the characterization of an alloreactive HLA-E-restricted CD8 T cell population that was detected in the PBL of a kidney transplant patient after its CMV conversion. This monoclonal CD8 T cell population represents a sizable fraction in the blood (3% of PBL) and is characterized by an effector-memory phenotype and the expression of multiple NK receptors. Interestingly, these unconventional T cells display HLA-E-dependent reactivity against peptides derived from the leader sequences of both various HCMV-UL40 and allogeneic classical HLA-I molecules. Consequently, while HLA-E-restricted CD8 T cells have potential to contribute to the control of CMV infection in vivo, they may also directly mediate graft rejection through recognition of peptides derived from allogeneic HLA-I molecules on graft cells. Therefore, as HLA-E expression in nonlymphoid organs is
HLA typing, along with ABO (blood type) grouping, is used to provide evidence of tissue compatibility. The HLA antigens expressed on the surface of the lymphocytes of the recipient are matched against those from various donors. Human leukocyte antigen typing is performed for kidney, bone marrow, liver, pancreas, and heart transplants. The probability that a transplant will be successful increases with the number of identical HLA antigens. Graft rejection occurs when the immune cells (T-lymphocytes) of the recipient recognize specific HLA antigens on the donors organ as foreign. The T-lymphocytes initiate a cellular immune response that result in graft rejection. Alternatively, T-lymphocytes present in the grafted tissue may recognize the host tissues as foreign and produce a cell-mediated immune response against the recipient. This is called graft versus host disease (GVHD), and it can lead to life-threatening systemic damage in the recipient. Human leukocyte antigen testing is performed to ...
MAVMAPRTLL LLLSGALALT QTWAGSHSMR YFYTSVSRPG RGEPRFIAVG YVDDTQFVRF DSDAASQRME PRAPWIEQEG PEYWDQETRN VKAQSQTDRV DLGTLRGYYN QSEDGSHTIQ IMYGCDVGPD GRFLRGYRQD AYDGKDYIAL NEDLRSWTAA DMAAQITKRK WEAAHAAEQQ RAYLEGRCVE WLRRYLENGK ETLQRTDPPK THMTHHPISD HEATLRCWAL GFYPAEITLT WQRDGEDQTQ DTELVETRPA GDGTFQKWAA VVVPSGEEQR YTCHVQHEGL PKPLTLRWEL SSQPTIPIVG IIAGLVLLGA VITGAVVAAV MWRRKSSDRK GGSYTQAASS DSAQGSDVSL TACKV ...
HLA-B belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. Class I molecules play a central role in the immune system by presenting peptides derived from the endoplasmic reticulum lumen. They are expressed in nearly all cells. The heavy chain is approximately 45 kDa and its gene contains 8 exons. Exon 1 encodes the leader peptide, exon 2 and 3 encode the alpha1 and alpha2 domains, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region and exons 6 and 7 encode the cytoplasmic tail. Polymorphisms within exon 2 and exon 3 are responsible for the peptide binding specificity of each class one molecule. Typing for these polymorphisms is routinely done for bone marrow and kidney transplantation. Hundreds of HLA-B alleles have been described. [provided by RefSeq, Jul 2008 ...
HLA-A belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. Class I molecules play a central role in the immune system by presenting peptides derived from the endoplasmic reticulum lumen. They are expressed in nearly all cells. The heavy chain is approximately 45 kDa and its gene contains 8 exons. Exon 1 encodes the leader peptide, exons 2 and 3 encode the alpha1 and alpha2 domains, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region, and exons 6 and 7 encode the cytoplasmic tail. Polymorphisms within exon 2 and exon 3 are responsible for the peptide binding specificity of each class one molecule. Typing for these polymorphisms is routinely done for bone marrow and kidney transplantation. Hundreds of HLA-A alleles have been described. [provided by RefSeq, Jul 2008 ...
Edits to https://fairsharing.org/FAIRsharing.e28v7g by The FAIRsharing Team at 18:13, 10 Feb 2020 (approved): supportLinks has been modified: Before: FAQ,http://www.ebi.ac.uk/ipd/imgt/hla/help/faq.html contact form,http://www.ebi.ac.uk/support/hla.php email,[email protected] help,https://www.ebi.ac.uk/ipd/imgt/hla/access.html online documentation,https://www.ebi.ac.uk/ipd/imgt/hla/nomenclature/index.html online documentation,https://www.ebi.ac.uk/ipd/imgt/hla/dpb.html online documentation,https://www.ebi.ac.uk/ipd/imgt/hla/intro.html online documentation,https://www.ebi.ac.uk/ipd/imgt/hla/citations.html online documentation,https://www.ebi.ac.uk/ipd/imgt/hla/stats.html After: FAQ,https://www.ebi.ac.uk/ipd/imgt/hla/help/faq.html contact form,http://www.ebi.ac.uk/support/hla.php help,https://www.ebi.ac.uk/ipd/imgt/hla/access.html online documentation,https://www.ebi.ac.uk/ipd/imgt/hla/nomenclature/index.html online documentation,https://www.ebi.ac.uk/ipd/imgt/hla/dpb.html online ...
DISI : Determining class I human leukocyte antigens (HLA) antigens on specimens for those who have become refractory to platelet transfusions and identify potential disease association
Reactome is pathway database which provides intuitive bioinformatics tools for the visualisation, interpretation and analysis of pathway knowledge.
Reactome is pathway database which provides intuitive bioinformatics tools for the visualisation, interpretation and analysis of pathway knowledge.
The human leukocyte antigen (HLA) is a gene complex encoding the major histocompatibility complex (MHC) proteins in humans. The complex is located within the 6p21.3 region on the short arm of human chromosome 6. These genes are highly polymorphic, which means there are many different HLA alleles. More information of HLA and HLA alleles can be found on hla.alleles.org and The IMGT/HLA Database. This is an attempt to plot the rapidly increasing number of HLA alleles over time, with data directly parsed from the IMGT/HLA Statistics page. This notebook file, described below, can be downloaded here.. ...
We describe here the isolation and sequencing of a previously uncharacterized HLA class I gene. This gene, HLA-5.4, is the third non-HLA-A,B,C gene characterized whose sequence shows it encodes an intact class I protein. RNase protection assays with a probe specific for this gene demonstrated its expression in B lymphoblastoid cell lines, in resting T cells, and skin cells, while no mRNA could be detected in the T cell line Molt 4. Consistent with a pattern of expression different from that of other class I genes, DNA sequence comparisons identified potential regulator motifs unique to HLA-5.4 and possibly essential for tissue-specific expression. Protein sequence analysis of human and murine class I antigens has identified 10 highly conserved residues believed to be involved in antigen binding. Five of these are altered in HLA-5.4, and of these, three are nonconservative. In addition, examination of the HLA-5.4 DNA sequence predicts that the cytoplasmic segment of this protein is shorter than ...
Present knowledge regarding the HLA system and the association between HLA antigens and insulin-dependent type 1 diabetes mellitus (IDDM) is reviewed. The heterogeneity of diabetes, immunogenetically speaking, is emphasized. Results are reported for HLA typing in 18 cases of known IDDM recently diag …
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This test looks at the human leukocyte antigens (HLA) in your blood. This test is used if you need an organ or stem-cell transplant, to find an organ or stem cells that are as close to yours as possible. An improper match if you need a stem-cell transplant could cause the stem cells to harm you. A mismatched organ transplant can cause the organ to fail and be rejected. HLAs are proteins found on the surface of most of the cells in your body. They signal to your immune system which cells are parts of your body and which cells are potentially harmful organisms. They play an important role in protecting you from infections, but they also make organ transplants more difficult. HLAs are also involved in autoimmune diseases. These are diseases in which the body attacks its own tissues. The HLA test can be used to identify these diseases. ...
The extraordinary polymorphism of human leukocyte antigens (HLA) poses a question as to how this remarkable diversity arose and is maintained. The explanation that infectious pathogens are largely responsible is theoretically attractive but clear and consistent associations between HLA alleles and major infectious diseases have rarely been identified. Large case-control studies of HLA types in African children with severe malaria indicate that HLA associations with this parasitic infection do exist and it is becoming possible to investigate the underlying mechanisms by identification of peptide epitopes in parasite antigens. Such analysis reveals how the magnitude and detectability of HLA associations may be influenced by numerous genetic and environmental factors. These complex interactions will give rise to variation over time and space in the selective pressures exerted by infectious diseases and this fluctuation may, in itself, contribute to the maintenance of HLA polymorphism.
EIAab can provide you Human HLA class I histocompatibility antigen, B-53 alpha chain ELISA Kit(Human HLA-B ELISA Kit) with more favourable price and high quality.
EIAab can provide you Human HLA class I histocompatibility antigen, B-39 alpha chain ELISA Kit(Human HLA-B ELISA Kit) with more favourable price and high quality.
A short but detailed and well-documented primer on the histocompatibility leukocyte antigen system (HLA system); history; components; linkage relationships; homologous systems in infrahuman species; biochemistry; correlations with disease; clinical applications; biology; methods. Two hundred forty-nine references. A good starting point for the student or older clinician who needs a clear introduction to this field. ...
Overall, there was no evidence of contamination among the cell lines tested with clean homozygous or heterozygous combinations observed in all loci analyzed. SBT of HLA class I and HLA class II loci are reported in Table 2 and 3 respectively. Information about the HLA typing of the cell lines is also available through the Molecular Targets URL: http://dtp.nci.nih.gov/mtargets/mt_index.html. Approximately 17% of the cell lines (10 out of 58 including: T47D, SNB-19, U251, KM12, RPMI-8226, EKVX, NCI-H23, NCI-H322M, A498, ACHN and TK-10) exhibited a pseudo-homozygous pattern suggestive of complete loss of heterozygosity encompassing the HLA class I and HLA class II regions. This frequency is close to the loss of haplotype that we originally described for melanoma cell lines generated at the National Cancer Institute (Bethesda, MD) [38, 39] and subsequently observed in other cancers [40, 41]. We conclude that this is an unlikely representative of patients homozygosity because complete HLA class I ...
Laboratory abnormalities in ME/CFS include abnormal SIgA; weakly positive IgG3 (linked to gastrointestinal tract disorders); positive IgM; increased T4:T8 ratio (which always corresponds with disease severity); very low numbers of NK cells, with decreased cytolytic activity; low levels of circulating immune complexes (two-thirds of ME patients have insoluble circulating immune complexes); autoantibodies (especially antinuclear and smooth muscle); a particular HLA antigen expression; PCR evidence of abnormalities in muscle; a positive water loading test with erratic arginine-vasopressin release; a significant prolactin release in response to a single buspirone challenge; positive SPECT scans (which show reduced blood flow through the brain stem in a particular pattern not found in any other illness or disease process apart from ME/CFS - QJMed 1995:88:767-773); abnormal fMRI scans; abnormal EEG (80% of ME patients show prolonged jitter); a positive VP1 test; positive mast cells; low pancreatic ...
TY - JOUR. T1 - The occurrence of HLA‐B46 in two Caucasoid families. AU - Hart, J. M.. AU - Zemmour, J.. AU - Schmeckpeper, B. J.. AU - Parham, P.. AU - Wood, W. W.. AU - Hopkins, K. A.. AU - Leffell, M. S.. AU - Bias, W. B.. PY - 1993/1. Y1 - 1993/1. KW - DNA sequence of Caucasoid HLA‐B46. KW - HLA distribution in populations. KW - HLA‐B46 in non‐orientals. KW - gene flow of HLA antigens. UR - http://www.scopus.com/inward/record.url?scp=0027447465&partnerID=8YFLogxK. UR - http://www.scopus.com/inward/citedby.url?scp=0027447465&partnerID=8YFLogxK. U2 - 10.1111/j.1399-0039.1993.tb01977.x. DO - 10.1111/j.1399-0039.1993.tb01977.x. M3 - Article. C2 - 8456442. AN - SCOPUS:0027447465. VL - 41. SP - 47. EP - 50. JO - HLA. JF - HLA. SN - 2059-2302. IS - 1. ER - ...
The growing list of associations between idiosyncratic adverse drug reactions and variants of the Human Leukocyte Antigen (HLA) molecule is suggestive of specific interactions between the causative drug and the associated HLA molecule. Currently, most of our understanding of how HLA molecules stimulate drug specific T cell responses comes from observing the conditions necessary for T cell stimulation (e.g. specific HLA, time for drug metabolism, constant drug presence, antigen processing). Whilst this provides indirect evidence of the presence of immunogenic ligands on the cell surface it does not determine the precise nature of the immunogenic HLA ligand(s). Using a mixture of mass spectrometry (MS) and structural biology techniques we recently defined the mode of interaction between abacavir and HLA-B*57:01. We showed how, by occupying the antigen binding cleft, abacavir changes the array of peptides bound by the HLA molecule, furnishing circulating T cells with numerous novel, potentially ...
1DUZ: The structure and stability of an HLA-A*0201/octameric tax peptide complex with an empty conserved peptide-N-terminal binding site.
We describe here the isolation and sequencing of a previously uncharacterized HLA class I gene. This gene, HLA-5.4, is the third non-HLA-A,B,C gene characterized whose sequence shows it encodes an intact class I protein. RNase protection assays with a probe specific for this gene demonstrated its expression in B lymphoblastoid cell lines, in resting T cells, and skin cells, while no mRNA could be detected in the T cell line Molt 4. Consistent with a pattern of expression different from that of other class I genes, DNA sequence comparisons identified potential regulator motifs unique to HLA-5.4 and possibly essential for tissue-specific expression. Protein sequence analysis of human and murine class I antigens has identified 10 highly conserved residues believed to be involved in antigen binding. Five of these are altered in HLA-5.4, and of these, three are nonconservative. In addition, examination of the HLA-5.4 DNA sequence predicts that the cytoplasmic segment of this protein is shorter than ...
9 Mer Peptide (RL9) From Histone H2A.xBeta-2-microglobulinHLA class I histocompatibility antigen, alpha chain GLeukocyte immunoglobulin-like receptor subfamily B member 1
We describe here the isolation and sequencing of a previously uncharacterized HLA class I gene. This gene, HLA-5.4, is the third non-HLA-A,B,C gene characterize
Bio-Rad HLA serology products are used for the detection of either human HLA antigens or HLA antibodies in a complement-dependent microlymphocytotoxicity (MLCT) test
The high diversity of HLA binding preferences has been driven by the sequence diversity of short segments of relevant pathogenic proteins presented by HLA molecules to the immune system. To identify possible commonalities in HLA binding preferences, we quantify these using a novel measure termed targeting efficiency, which captures the correlation between HLA-peptide binding affinities and the conservation of the targeted proteomic regions. Analysis of targeting efficiencies for 95 HLA class I alleles over thousands of human proteins and 52 human viruses indicates that HLA molecules preferentially target conserved regions in these proteomes, although the arboviral Flaviviridae are a notable exception where nonconserved regions are preferentially targeted by most alleles. HLA-A alleles and several HLA-B alleles that have maintained close sequence identity with chimpanzee homologues target conserved human proteins and DNA viruses such as Herpesviridae and Adenoviridae most efficiently, while all ...
Individual leucocyte antigen (HLA) compatibility may be the key determining the incident of graft\vs\web host disease (GVHD) in sufferers. replies, correlate with markers involved with GVHD and will potentially end up being useful in the analysis of the natural processes involved with this disease. mismatched pairs. We discovered that a lot of genes linked to immune function were differentially regulated; these genes were also found to be associated with GVHD. The most highly upregulated genes were IFN\inducible genes and IFN neutralisation in MLCs abrogated their induction. The microRNA\155 (miR\155) was also found to be significantly induced in the mismatched setting but its induction was not diminished by blocking IFN. We are proposing that measuring gene expression in MLC could be a simpler and faster method of identifying functional incompatibilities between potential donorCrecipient pairs that are not detected by DNA typing techniques, compared with the traditional cellular assays. ...
Based on the structural similarity between B cell receptor and immunoglobulin binding sites, it is postulated that HLA-specific B cells should bind to HLA molecules with specificity comparable to that of the secreted immunoglobulin. Indeed, identification of HLA specifc B cells by staining through binding of the B cell receptor using 﫿uorescently labeled tetramers of identified HLA class I specifcities has been described [13]. A different approach is to utilize commercially available single antigen coated, color-coded microspheres, multiplexed in an assay that is currently the mainstay of soluble antibody detection in the circulation [14, 15, 16]. However, this powerful assay has also highlighted the challenges of cross- and poly-reactivity of allo-antibodies [17, 18] have recently described a method utilizing single HLA coated beads to enumerate HLA committed B cells [18]. Class I HSB identified in non-transplanted individuals were described by [19] who identified HLA specific B cells using ...
To our knowledge, this is the first large-scale reverse-genomics study in which the results of a genetic analysis were used to directly inform the selection and subsequent testing of particular viral Ags. Overall, we were able to provide immunological support for 190 HLA-associated polymorphisms in subtype B HIV-1 as being sites of direct T cell recognition in vivo based on ex vivo IFN-γ responses in the appropriate HLA background. This was 58% of the HLA associations tested in the study, representing an increase from only 35% that could have been explained by well-characterized published CD8 T cell epitopes alone, prior to any cellular testing. For nine high-probability epitopes, there was a sufficiently frequent HLA type to show that the most likely HLA restriction of the epitopic response in the cohort matched that of the prediction, and there was sufficient frequency of testing and responses in ≥40% of cases to give the best level of evidence for immunoreactivity. An additional set of ...
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There is reasonable evidence that genetic pressures help to insure that offspring are different in the HLA loci from the parents. The high variability of HLA molecules enlarges the kinds of pathogens that can be recognized and eliminated by an immune response within a population. However, a disadvantage of high HLA specificity occurs during organ transplants. The body recognizes the organ as non-self, and launches an immune response against it. Proteins from HLA genes are the cause of transplant rejections, and HLA genotypes must be partially matched to improve outcomes of organ transplants. Finally, studies on many species show that mating preference and odor is somehow related to MHC alleles. Female mice prefer to mate with males having different MHC loci. The immune advantage of MHC diversity is apparently selected for during mating.. ...
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MHC (major histocompatibility complex), also known as HLA (human leukocyte antigen) is the entire set of genes that code for proteins involved in antigen presentation. Very simply, you could think of HLA as analogous to a flagpole waving a flag to notify the immune system of a particular antigen. Hey Immune System, is this self or non-self? or in other words, Are you foreign??? HLA has also been described as a billboard advertising peptides to the mighty CD8+ and CD4+ T cells as they provide immune surveillance.. MHC Class I and Class II have distinct roles and a few differences include the following:. Binding Groove The peptide-binding groove or binding pocket where the antigen sits is unique between Class I and Class II. It has been established that in order for a T-cell to be alerted, the peptide has to be stably bound within this cleft. Hence, peptide-binding affinity is a critical factor in putative neoantigen candidate criteria. For MHC class I, the peptides are ~8 to 11 amino ...
Once the indication to transplant has been confirmed, patients and their relatives must be informed on how the transplant is performed. Patients should understand that identifying a stem cell donor is an absolute prerequisite to perform a transplant. Accordingly, patients should be informed about the human leukocyte antigen (HLA) genetic system, its specificity for each individual, how it is inherited by parents according to the Mendelian laws, and what is the probability to find a compatible donor in the family group. Understanding the HLA system is crucial to explain why the use of a HLA family-matched sibling donors is considered standard and when such a sibling is not available; an international search has to be performed to identify a HLA-compatible unrelated donor. It is important to underline that more than 30 million of potentially available donors are registered by the World Marrow Donor Association (WMDA), and the probability to find a compatible donor is between 50 and 80% according ...
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Moving back to solid science, one of the major challenges when it comes to transplantation is rejection. This is when the immune system identifies the transplant as foreign tissue and launches an assault on it, causing damage that can end in the death of the organ, and therefore possibly the recipient. Rejection is more likely when the donor and recipient are a poor tissue match. This matching is largely based on a set of proteins called Human Leukocyte Antigens (HLAs) that are found on the surface of every single cell in our bodies. Our HLAs are like a barcode that identify our cells as our own. A transplanted organ is exceptionally unlikely to be a perfect match, so its HLAs are different from our own cells. If they are too different, the immune system successfully plays spot-the-difference and targets the transplant for destruction. We can try and mitigate this by using drugs that suppress the immune system, but this immunosuppression puts the recipient at higher risk of developing ...
Fifty years since the first description of an association between HLA and human disease, HLA molecules have proven to be central to physiology, protective immunity and deleterious, disease-causing autoimmune reactivity. Technological advances have enabled pivotal progress in the determination of the molecular mechanisms that underpin the association between HLA genetics and functional outcome. Here, we review our current understanding of HLA molecules as the fundamental platform for immune surveillance and responsiveness in health and disease. We evaluate the scope for personalized antigen-specific disease prevention, whereby harnessing HLA-ligand interactions for clinical benefit is becoming a realistic prospect.
The main objective of the study is to evaluate which proteins are presented by the HLA complex of solid tumors. Secondary objectives include to evaluate whether an immune response specific to these proteins has actually been provoked and whether there is a similar immune response following recurrence. Real specimens will be evaluated using PCR and mass spectrometry ...
www.MOLUNA.de HLA and Disease Associations [4196310] - The human leukocyte antigen (HLA) or tissue types are the products of a rapidly developing field of knowledge within the last 20 years. In the early stages of the research many investigators suspected the existence of a complex series of transplantation antigens, but it was widely believed that these antigens
HLA DR小鼠单克隆抗体[TAL 1B5](ab20181)可与人样本反应并经WB, IHC, Flow Cyt实验严格验证,Abcam HLA DR抗体被7篇文献引用并得到4个独立的用户反馈。所有产品均提供质保服务,中国75%以上现货。
The Many Faces of Human Leukocyte Antigen-G: Relevance to the Fate of Pregnancy. . Biblioteca virtual para leer y descargar libros, documentos, trabajos y tesis universitarias en PDF. Material universiario, documentación y tareas realizadas por universitarios en nuestra biblioteca. Para descargar gratis y para leer online.
TY - JOUR. T1 - HLA antigens in japanese patients with systemic lupus erythematosus. AU - Hashimoto, H.. AU - Nishimura, Y.. AU - Dong, R. P.. AU - Kimura, A.. AU - Sasazuki, Takehiko. AU - Yamanaka, K.. AU - Tokano, Y.. AU - Murashima, A.. AU - Kabasawa, K.. AU - Hirose, S.. PY - 1994/1/1. Y1 - 1994/1/1. N2 - To determine the association of HLA antigens with SLE and the clinical findings of the disease, HLA antigens were tested in 58 Japanese patients with SLE, who fulfilled the ARA diagnostic criteria, along with 97 normal controls. HLA class I and II antigens were typed serologically using the antisera provided by the 11th HLA Workshop. Among the HLA class II antigens, further DRB, DQ and DP alleles were defined by DNA typing using the PCR/SSOP method. There were significantly more SLE patients with HLA-B39, DRB1*1501, DRB5*0101 and DQB1*0602 than normal controls. This result suggested that the haplotype of HLA-DRB1*1501-DRB5*0101-DQA1*0102-DQB1*0602 consists of the SLE-associated MHC markers ...
TY - JOUR. T1 - Functional interaction between human histocompatibility leukocyte antigen (HLA) class II and mouse CD4 molecule in antigen recognition by T cells in HLA-DR and DQ transgenic mice. AU - Yamamoto, Ken. AU - Fukui, Yoshinori. AU - Esaki, Yukio. AU - Inamitsu, Takeshi. AU - Sudo, Tohm. AU - Yamane, Kazuaki. AU - Kamikawaji, Nobuhiro. AU - Kimura, Akinori. AU - Sasazuki, Takehiko. PY - 1994/7/1. Y1 - 1994/7/1. N2 - Studies in vitro have suggested that a species barrier exists in functional interaction between human histocompatibility leukocyte antigen (HLA) class II and mouse CD4 molecules. However, whether mouse CD4+ T cells restricted by HLA class II molecules are generated in HLA class II transgenic mice and respond to peptide antigens across this barrier has remained unclear. In an analysis of T cell responses to synthetic peptides in mice transgenic for HLA-DR51 and - DQ6, we found that DR51 and DQ6 transgenic mice acquired significant T cell response to influenza ...
Allogeneic bone marrow transplantation is an effective postremission strategy for patients with acute myelogenous leukemia (AML) in first complete remission (CR). The value of administering consolidation chemotherapy before human leukocyte antigen (HLA)-identical sibling transplantation is not established. Outcomes of patients with AML in first CR receiving no consolidation therapy, standard-dose cytarabine consolidation therapy, and high-dose cytarabine consolidation therapy before HLA-identical sibling transplantation were compared. Five-year treatment-related mortality rates were 30% (95% confidence interval [CI], 18% to 42%) in patients receiving no consolidation chemotherapy, 22% (95% CI, 17% to 28%) in those receiving standard-dose cytarabine consolidation, and 24% (95% CI, 17% to 31%) in those receiving high-dose cytarabine (P = NS). Five-year cumulative incidences of relapse were 19% (10% to 30%), 21% (16% to 27%), and 17% (11% to 24%), respectively (P = NS). Five-year probabilities of ...
Background: TH2-biased immune responses are important in allergy pathogenesis. Mechanisms of allergen-specific immunotherapy (SIT) might include the induction of regulatory T cells (Tregs) and immunoglobulin (Ig) G4 blocking antibodies, a reduction in the number of effector cells, and skewing of the cytokine profile towards a TH1-polarized immune response. We investigated the effects of SIT on T cells, on immunomodulation of human leukocyte antigen (HLA)-G, which has been associated with allergy, on regulatory cytokine expression, and on serum allergen-specific antibody subclasses (IgE and IgG4). Methods: Eleven birch and/or grass pollen-allergic patients and 10 healthy nonatopic controls were studied before and during SIT. Tregs, chemokine receptors, soluble HLA-G (sHLA-G), Ig-like transcript (ILT) 2, specific IgE, and IgG4 were studied. Peripheral blood mononuclear cells (PBMCs) were stimulated with pollen extract in vitro and immune factors were evaluated. Results: During SIT, the main ...
ENCODES a protein that exhibits peptide antigen binding (ortholog); INVOLVED IN alpha-beta T cell activation involved in immune response (ortholog); antigen processing and presentation of endogenous peptide antigen via MHC class Ib (ortholog); antigen processing and presentation of exogenous peptide antigen via MHC class Ib (ortholog); FOUND IN external side of plasma membrane (ortholog); MHC class Ib protein complex (ortholog); INTERACTS WITH flavonoids; 1,2-dimethylhydrazine (ortholog); 2,3,7,8-tetrachlorodibenzodioxine (ortholog)
Abstract. Autoimmune hepatitis (AIH) can arise de novo after liver transplantation (LT) for non-autoimmune liver diseases. Considering the identical features of de novo AIH after LT and classical AIH, as well as the importance of anti-human leukocyte antigen (HLA) antibodies in graft rejection, we investigated the presence of circulating anti-HLA class II antibodies in the sera of 35 patients with AIH, 30 patients with primary biliary cirrhosis (PBC), and 30 healthy donors using fluorescent dye-impregnated beads bound to HLA molecules. We then investigated the allele specificity of the antibodies and identified the HLA alleles in each patient using DNA-based HLA typing. We also examined HLA class II expression in liver samples using immunohistochemistry. Anti-HLA class II antibodies were detected significantly more frequently in the patients with AIH (88.1%) than in the patients with PBC (33.3%) or in the healthy donors (13.3%) (both P ,0.01). We confirmed that the anti-HLA class II antibodies ...
Background: HLA mismatches of the donor and recipient causing de novo donor-specific HLA antibodies (dnDSA) are a risk factor for immunologic complications and poor graft survival. Evaluating histocompatibility based on HLA epitopes may improve outcomes after kidney transplantation and better prevent sensitization than classical matching of HLA class I and II antigens. To date there is very few data examining clinical outcomes based on HLA epitope matching.. Patients and methods: 2,787 consecutive adult kidney transplants performed 1995-2015 without preformed DSA have been included. De novo DSA were detected by Luminex single antigen assay. HLAMatchmaker score was calculated as the weighted sum of eplets of high resolution patient and donor typing.. Results: The distribution of the HLA epitope mismatch (assessed by the HLAMatchmaker algorithm) and the correlation to the count of mismatched A,B,C,DR,DQ-HLA molecules is shown in Fig.1a and Fig.1b. Categorization by HLAMatchmaker epitopes ...
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Looking for online definition of Human Leukocyte Antigen Test in the Medical Dictionary? Human Leukocyte Antigen Test explanation free. What is Human Leukocyte Antigen Test? Meaning of Human Leukocyte Antigen Test medical term. What does Human Leukocyte Antigen Test mean?
Publikationen (2008-2015) ELISA-based cross-matching allowing the detection of emerging donor-specific anti-HLA antibodies through the use of stored donors cell lysates; Schlaf G, Stöhr K, Rothhoff A, Altermann W; Case Rep. Transplant.; 2015; 763157; Epub 2015 Oct 15th. Detection of post-transplant anti-HLA donor-specific antibodies through the use of stored donors cell lysates and solid phase-based cross-matching; Schlaf G, Pistorius I, Altermann W; J. Clin. Cell. Immunol.; 2015; 6; 383-391. Solid phase-based cross-matching in order to avoid kidney allografting against donor-specific anti-HLA antibodies: Long term experience with a procedure allowing highly reliable diagnoses; Schlaf G, Pollok-Kopp B, Altermann W; Austin J. Nephrol. Hypertens.; 2014, 1(6); Epub November 2014, Printversion folgt. Solid phase-based cross-matching as solution for kidney allograft recipients pretreated with therapeutic antibodies; Schlaf G, Apel S, Wahle A, Altermann W; BioMed Res. Int.; 2014; Epub September ...
Polymorphisms located within the MHC have been linked to many disease outcomes by mechanisms not yet fully understood in most cases. Variants located within untranslated regions of HLA genes are involved in allele-specific expression and may therefore underlie some of these disease associations.. We determined sequences extending nearly 2 kb upstream of the transcription start site for 68 alleles from 57 major lineages of classical HLA class I genes. The nucleotide diversity within this promoter segment roughly follows that seen within the coding regions, with HLA-B showing the highest (?1.9%), followed by HLA-A (?1.8%), and HLA-C showing the lowest diversity (?0.9%). Despite its greater diversity, HLA-B mRNA expression levels determined in 178 European Americans do not vary in an allele- or lineage-specific manner, unlike the differential expression levels of HLA-A or HLA-C reported previously.. Close proximity of promoter sequences in phylogenetic trees is roughly reflected by similarity of ...
Kamla-Raj 2003 Int J Hum Genet, 3(1): 39-43 (2003). HLA DRB1 and DQB1 gene diversity in Maratha community from Mumbai India. U. Shankarkumar *, J. P. Devaraj, K. Ghosh and D. Mohanty. HLA Department, Institute of Immunohaematology (ICMR), 13th Floor, NMS Bldg,. K.E.M.Hospital, Parel, Mumbai 400 012, Maharashtra, India. Email: [email protected] Key Words HLA; caste; maratha; population Abstract Indian Population exhibits not only a wide variety of ethnic but also great cultural and linguistic diversity. In the present study 113 unrelated Marathas residing in Mumbai, Maharastra, (Western India) were studied for HLA DRB1 and HLA DQB1 locus antigen profiles. The HLA antigens were identified using commercially procured PCR- SSP typing kits. The genotype frequency, haplotype frequency and Linkage disequilibrium estimates were calculated following the standard methods. The HLA antigen frequencies of HLA DRB1*02, DRB1*15, DRB1*0701, DQB1*06 and DQB1*0203 were increased while that of HLA ...
TY - JOUR. T1 - Effect of matching of class I HLA alleles on clinical outcome after transplantation of hematopoietic stem cells from an unrelated donor. AU - Sasazuki, Takehiko. AU - Juji, Takeo. AU - Morishima, Yasuo. AU - Kinukawa, Naoko. AU - Kashiwabara, Hidehiko. AU - Inoko, Hidetoshi. AU - Yoshida, Takato. AU - Kimura, Akinori. AU - Akaza, Tatsuya. AU - Kamikawaji, Nobuhiro. AU - Kodera, Yoshihisa. AU - Takaku, Fumimaro. AU - Nose, Yoshiaki. AU - Ono, Takashi. AU - Sakamaki, Takeo. AU - Kato, Shunichi. AU - Akiyama, Yuichi. AU - Okamoto, Shinichiro. AU - Dohy, Hiroo. AU - Harada, Mine. AU - Asano, Shigetaka. PY - 1998/10/22. Y1 - 1998/10/22. N2 - Background. The requirements with respect to HLA compatibility and the relative importance of matching for individual class I and class II HLA alleles in the transplantation of hematopoietic stem cells from unrelated donors have not yet been established. Methods. We performed retrospective DNA typing of alleles at 11 polymorphic loci of HLA genes ...
Transplantation with hematopoietic stem cells (HSC) from a donor with a single human leukocyte antigen (HLA) mismatch can be proposed to those patients lacking an HLA identical sibling donor or an unrelated donor matched for the HLA-A, -B, -C, DRB1, DQB1 loci. Incompatibilities at HLA classes I and II loci are associated with an increased risk of graft-versus-host disease (GVHD) and mortality, although no consensus exists yet on the relative importance of specific allele disparities on clinical outcome. Donor search algorithms are now complicated by the growing number of new HLA alleles, in particular those that differ outside the peptide-binding site of the HLA molecules. We report here an in vitro cellular assay to quantify CD8+CD137+ alloreactive cytotoxic T lymphocytes (CTLs) in a one-way mixed lymphocyte reaction. Two unique combinations with a single HLA mismatch in the HLA-B44 serotype differing by one amino acid in the α3 domain were investigated. We show that the B*44:27 versus B*44:02 ...
The role of anti-HLA antibodies in renal transplantation. Human leukocyte antigens (HLA) are a highly polymorphic system of glycoproteins that have a functional role in the presentation of peptides to the immune system.1,2 HLA class I molecules are expressed on most nucleated cells, while HLA class II antigens are generally found on professional antigen-presenting cells.3 HLA class II antigens can also be upregulated on kidney cells.4 As a highly polymorphic system, however, specific HLA alleles can become the targets of antibody responses in people sensitised to foreign HLA molecules during pregnancy, transfusion of blood products, or organ transplantation.1,5,6 Humoral responses or sensitisation to HLA are major barriers to solid organ transplantation.1,7 Sensitised patients are more likely to crossmatch positive with potential donors and have a reduced chance of receiving a renal transplant.8 The detection of anti-HLA antibodies helps to predict both the function and survival of transplant ...
TY - JOUR. T1 - Personalized peptide arrays for detection of HLA alloantibodies in organ transplantation. AU - Liu, Pan. AU - Souma, Tomokazu. AU - Wei, Andrew Zu Sern. AU - Xie, Xueying. AU - Luo, Xunrong. AU - Jin, Jing. PY - 2017/9/6. Y1 - 2017/9/6. N2 - In organ transplantation, the function and longevity of the graft critically rely on the success of controlling immunological rejection reactivity against human leukocyte antigens (HLA). Histocompatibility guidelines are based on laboratory tests of anti-HLA immunity, which presents either as pre-existing or de novo generated HLA antibodies that constitute a major transplantation barrier. Current tests are built on a single-antigen beads (SAB) platform using a fixed set of ~100 preselected recombinant HLA antigens to probe transplant sera. However, in humans there exist a far greater variety of HLA types, with no two individuals other than identical twins who can share the same combination of HLA sequences. While advanced technologies for HLA ...
Human immunodeficiency virus (HIV)-1 amino acid sequence polymorphisms associated with expression of specific human histocompatibility leukocyte antigen (HLA) class I alleles suggest sites of cytotoxic T lymphocyte (CTL)-mediated selection pressure and immune escape. The associations most frequently observed are between expression of an HLA class I molecule and variation from the consensus sequence. However, a substantial number of sites have been identified in which particular HLA class I allele expression is associated with preservation of the consensus sequence. The mechanism behind this is so far unexplained. The current studies, focusing on two examples of negatively associated or apparently preserved epitopes, suggest an explanation for this phenomenon: negative associations can arise as a result of positive selection of an escape mutation, which is stable on transmission and therefore accumulates in the population to the point at which it defines the consensus sequence. Such negative ...
The causal association between persistent human papillomavirus (HPV) infection and cervical cancer has been established, but the mechanisms that favor HPV persistence in cervical cells are still unknown. The diminished capability of the immune system to control and resolve HPV infection is one of several hypotheses. The tolerogenic protein HLA-G has shown aberrant expression in a variety of cancers, which has been suggested as a mechanism for tumor escape from immunosurveillance. In the present study we evaluate the role of epigenetic modification (promoter de-methylation) of the HLA-G gene on susceptibility to HPV infection and development of high-grade cervical lesions. A case-control study was carried out in Curitiba, Brazil, between February and June 2010. A total of 789 women aged 15-47 years were recruited: 510 controls with normal cervical cytology, and 279 cases with histologically confirmed cervical intraepithelial neoplasia grade 2 (CIN2, N = 150) or grade 3 (CIN3, N = 129). All women were
Clone REA672 recognizes the class I human leukocyte antigens (HLA) B and C. Expressed on the surface of most nucleated cells, class I molecules are heterodimeric molecules and consist of a type I integral membrane α heavy chain and soluble β2 microglobulin protein. The extracellular region of the heavy chain further consists of three domains, one of which comprises the peptide binding groove. Antigens binding to class I molecules are 8-10 amino acids long and play an important role in recognition of the virus infected and malignant cells by cytotoxic T lymphocytes (CTLs). In addition, class I molecules interact with NK cell receptors to modulate the activity of NK cells. Additional information: Clone REA672 displays negligible binding to Fc receptors. - USA
Author Summary HLA class I molecules are expressed on the cell surface of almost all cells of the human body in complex with short fragments (peptides) of cytosolic proteins, thereby providing a snapshot of the intracellular state of a cell to circulating CD8+ T cells. Several processes are involved in shaping the peptide ligand repertoire of an HLA class I molecule, which generally represents only a small fraction of the proteins available in the cytosol. In our work we addressed protein sampling by HLA class I molecules to answer two questions: 1) Which proteins are sampled by the antigen processing pathway and why, and 2) which peptides of a given protein are picked to represent the source protein on the cell surface? To this end we quantified the contribution of each process involved in peptide processing and presentation individually and combined them into a logistic regression model. This simple model enabled us to predict the sampling probability of self proteins and may aid in the identification
The Luminex-based human leukocyte antigen (HLA) antibody screening technology is widespread used in laboratories affiliated to kidney transplantation programs and enables both screening (i. feasible using Luminex-based Semagacestat SSO technology also. The test treatment begins with PCR amplification of the very most polymorphic area of the HLA course II gene using exon 2-particular primers for HLA-DRB1, HLA-DQB1, HLA-DQA1, and HLA-DPB1 (fig. ?(fig.2).2). For the HLA-A, HLA-B, and HLAC loci two PCR items within the most polymorphic exons 2 and 3 of the HLA course I loci are produced. The PCR item can be biotinylated, that allows it to become recognized by strepavidin-conjugated with PE (SAPE). Subsequently, PCR items are denaturated allowing rehybridization to complementary DNA probes destined to the microbeads. Each microbead blend includes positive and negative control probes essential for subtraction of non-specific background indicators and normalization of uncooked data. For cleaning ...
The human leukocyte antigen (HLA) system or complex is a gene complex encoding the major histocompatibility complex (MHC) proteins in humans. These cell-surface proteins are responsible for the regulation of the immune system in humans. The HLA gene complex resides on a 3 Mbp stretch within chromosome 6p21. HLA genes are highly polymorphic, which means that they have many different alleles, allowing them to fine-tune the adaptive immune system. The proteins encoded by certain genes are also known as antigens, as a result of their historic discovery as factors in organ transplants. Different classes have different functions:. HLAs corresponding to MHC class I (A, B, and C) present peptides from inside the cell. For example, if the cell is infected by a virus, the HLA system brings fragments of the virus to the surface of the cell so that the cell can be destroyed by the immune system. These peptides are produced from digested proteins that are broken down in the proteasomes. In general, these ...
Author: de Pavón-Vargas, María los Ángeles et al.; Genre: Journal Article; Published online: 2019-07-22; Keywords: HLA, Immunogenetics, Population genetics, Tlaxcala, Admixture; Title: Genetic diversity of HLA system in two populations from Tlaxcala, Mexico: Tlaxcala city and rural Tlaxcala
The Human Leukocyte Antigen (HLA) region located on the short arm...For many years HLA polymorphisms were typed by serological respons...,The,Past,,Present,and,Future,of,HLA,Typing,medicine,advanced medical technology,medical laboratory technology,medical device technology,latest medical technology,Health
Searching for mechanisms of natural resistance to HIV infection with which to guide HIV vaccine design, we have examined antibody responses to HLA class I antigens in children of HIV-1-infected mothers. Anti-HLA antibodies are known to block HIV infectivity in vitro and can be protective against SIV challenge in macaques immunized with purified class I HLA. It was hypothesized that alloantibody to maternal HLA in children might contribute to the prevention of mother-to-child transmission of HIV-1. In fact, a surprisingly high proportion of the children examined, 22%, were found to have antibody against class I alloantigens. This alloantibody, however, did not correlate with the HIV status of the children and was found in a similar proportion of children of HIV-negative mothers. The HLA specificity of the antibody was not correlated with noninherited maternal HLA alleles and occurred with a higher frequency in older children. This result suggests environmental factors, rather than exposure to ...
The MHC contains many HLA class 1 and class 1 like DNA sequences. These include the classical class 1 genes (HLA-A, -B and -C), the nonclassical class 1 genes (HLA-E, -F and -G) and the so-called Class 1 non-expressed or pseudogenes (HLA-H, -K, -J, -L and many others). Relative to the other Class 1 genes, the pseudogenes have been understudied, largely, (and as their name suggests) as a result of the assumption that these genes have no function. However, a recent study (Paganini et al, 2019) has identified that some HLA-H alleles have all the elements expected of a functional HLA class 1 gene. This begs the questions. Is HLA-H functional? What is its function? Could other pseudogenes (HLA Class 1, HLA Class 2, MIC and others) be functional on some haplotypes? Could these pseudogenes be mismatched in transplantation? If so - what is the impact ...
Supervisor: Prof. Dr. Barbara Seliger Specific aim(s)/topic(s). (1) Identification of HLA class I targeting miRs, their functional characterization and clinical significance. (2) Identification and characterization of the function of RNA-binding proteins controlling components of the antigen processing and presentation machinery. Background and significance. Hematologic and solid tumors have developed strategies to escape immune surveillance, including abnormalities in the classical HLA antigens, which are directly associated with metastatic properties, disease progression and poor patients survival. The underlying molecular mechanisms of altered HLA class I surface antigen expression are only rarely associated with structural alterations in components of the HLA class I antigen processing and presentation machinery (APM), but are often due to their deregulated expression, which can occur at the epigenetic, transcriptional and post-transcriptional level . Altered gene expression in tumors can be ...
The T cell co-receptor, CD8, binds to the alpha 3 domain of HLA class I (Salter, R.D., R.J. Benjamin, P.K. Wesley, S.E. Buxton, T.P.J. Garrett, C. Clayberger, A.M. Krensky, A.M. Norman, D.R. Littman, and P. Parham. 1990. Nature [Lond.]. 345:41; Connolly, J.M., T.A. Potter, E.M. Wormstall, and T.H. Hansen. 1988. J. Exp. Med. 168:325; and Potter, T.A., T.V. Rajan, R.F. Dick II, and J.A. Bluestone. 1989. Nature [Lond.]. 337:73). To identify regions of CD8 that are important for binding to HLA class I, we performed a mutational analysis of the CD8 molecule in the immunoglobulin (Ig)-like variable domain. Our mutational analysis was based on our finding that using a cell-cell adhesion assay murine CD8 (Lyt-2) did not bind to human class I. Since the interaction of human CD8 with HLA class I is species specific, we substituted nonconservative amino acids from mouse CD8 and analyzed the ability of the mutated CD8 molecules expressed in COS 7 cells to bind HLA class I-bearing B lymphoblastoid cells, UC. ...
Human MHC (HLA) genes are the most polymorphic within the human genome (27, 54). The large number of genotypes (alleles and haplotypes) defining these loci have been presumably maintained primarily through balancing selection by a variety of human diseases. Recent discoveries (37, 38) that human cytomegalovirus infection might play some role in the pathogenesis of adult gliomas make HLA genes highly relevant as candidates for association with this disease. Our systematic analyses of HLA and related variants (alleles, haplotypes, supertypes, lineages, diplotypes, and homozygosity) at chromosome 6p21.3 revealed three HLA markers independently associated (two positively and one negatively) with adult glioblastoma multiforme. These markers lie centromeric to the HLA-A locus and telomeric to the HLA-DRB1 locus, suggesting that HLA-B, HLA-C and perhaps other genes in the central MHC (HLA class III and class IV) region can mediate the occurrence of adult glioblastoma multiforme. The lack of a ...
The promiscuous presentation of epitopes by similar HLA class I alleles holds promise for a universal T-cell-based HIV-1 vaccine. However, in some instances, cytotoxic T lymphocytes (CTL) restricted by HLA alleles with similar or identical binding motifs are known to target epitopes at different frequencies, with different functional avidities and with different apparent clinical outcomes. Such differences may be illuminated by the association of similar HLA alleles with distinctive escape pathways. Using a novel computational method featuring phylogenetically corrected odds ratios, we systematically analyzed differential patterns of immune escape across all optimally defined epitopes in Gag, Pol, and Nef in 2,126 HIV-1 clade C-infected adults. Overall, we identified 301 polymorphisms in 90 epitopes associated with HLA alleles belonging to shared supertypes. We detected differential escape in 37 of 38 epitopes restricted by more than one allele, which included 278 instances of differential escape at the
Some people cannot develop type 1 diabetes; thats because they dont have the genetic coding that researchers have linked to type 1 diabetes. Scientists have figured out that type 1 diabetes can develop in people who have a particular HLA complex. HLA stands for human leukocyte antigen, and antigens function is to trigger an immune response in the body.. There are several HLA complexes that are associated with type 1 diabetes, and all of them are on chromosome 6.. Different HLA complexes can lead to the development of other autoimmune disorders, such as rheumatoid arthritis, ankylosing spondylitis, or juvenile rheumatoid arthritis. Like those conditions, type 1 diabetes has to be triggered by something-usually a viral infection.. What Can Trigger Type 1 ...
Hum Biol. 1994 Oct;66(5):823-42. Mountains and genes: population history of the Pyrenees. Calafell F, Bertranpetit J. Unitat dAntropologia, Facultat de Biologia, Universitat de Barcelona, Spain. The Pyrenees Mountains rise between France and Spain. At their western end live the Basques, one of the oldest populations in Europe. All the available genetic information (gene frequencies of blood groups, serum proteins, red cell enzymes, and HLA antigens) on Pyrenean populations has
We extract and present high-resolution HLA allele and haplotype frequency data available from the National Marrow Donor Program databases from four major U.S. census categories of race and ethnicity. Population-based high-resolution HLA frequencies defined on the basis of from one to five loci are p …
Background: During untreated, chronic HIV-1 infection, plasma viral load (VL) is a relatively stable quantitative trait that has clinical and epidemiological implications. Immunogenetic research has established various human genetic factors, especially human leukocyte antigen (HLA) variants, as independent determinants of VL set-point. Methodology/Principal Findings: To identify and clarify HLA alleles that are associated with either transient or durable immune control of HIV-1 infection, we evaluated the relationships of HLA class I and class II alleles with VL among 563 seroprevalent Zambians (SPs) who were seropositive at enrollment and 221 seroconverters (SCs) who became seropositive during quarterly follow-up visits. After statistical adjustments for non-genetic factors (sex and age), two unfavorable alleles (A*3601 and DRB1*0102) were independently associated with high VL in SPs ( ...
Immunogenetic studies in various diseases provide potential genetic markers. We have studied the incidence of HLA A, B, C, DR and DQ loci antigen in Rh (D) antigen isoimmunized mothers compared to those nonimmunized isoimmunized Rh negative mothers. Seventy six mothers who were immunized to Rh (D) antigen due to pregnancy (responders) and fifty four mothers who did not develop Rh (D) isoimmunization despite positive pregnancies (nonresponders) were selected for the study. Standard methods of serological HLA typing, ABO and Rh (D) groups, and screening for Rh D antibodies were used. 392 unrelated individuals from the population were compared as controls. In addition 45 unrelated individuals from the same population were typed for HLA DRB and DQB gene using PCR-SSP kits. The genotype frequencies of HLA A2, A3, A28, B13, B17, B35, B52, B60, Cw2, Cw6, DR4, and DQ3 were significantly increased, while the frequencies of the HLA A11, A29, A31, B7, B37, B51, Cw1 and DR9 were decreased in the responder ...
Our analysis indicated that MICA-129 mismatches significantly influence survival outcome in unrelated HSCT. Because of the strong linkage within extended HLA haplotypes, and with HLA-B in particular, MICA mismatches, and consequentially also MICA-129 mismatches, are observed with increasing frequency when the number of HLA mismatches rises.21 As HLA matching must be considered as a confounder, we designed our analysis to obtain estimates for each HLA match group. Within each of these groups, OS rates were lower if a MICA-129 mismatch was present, whereas transplant pairs mismatched for MICA and matched for MICA-129 had similar survival rates as patients transplanted with MICA-matched donors. The differences were statistically significant for the 10/10 HLA as well as for the 9/10 HLA match groups (Figures 1 and 2; Table 2). The number of mismatches on 6 loci allele level, including MICA-129 to HLA-A, HLA-B, HLA-C, HLA-DRB1, and HLA-DQB1 matching, strongly correlated with OS. In previous studies, ...
The HLA Ligand Atlas is a comprehensive, tissue and HLA allele specific collection of HLA ligands, generated from more than 1,000 MS experiments.
The HLA Ligand Atlas is a comprehensive, tissue and HLA allele specific collection of HLA ligands, generated from more than 1,000 MS experiments.
The human leukocyte antigen (HLA) genes located within the human major histocompatibility complex on chromosome 6 are probably the most polymorphic functional genetic loci studied to date. The...
At the UMHS Histocompatibility Laboratory potential recipients are typed for HLA-A, B, C, DRB1,3,4,5, DQB1, loci by high resolution (allele level) molecular methods. The methods utilized for HLA typing are SSOP, SSP and/or SBT. In addition to the HLA high resolution typing patients are screened for preformed anti-HLA antibodies, the detection of anti-HLA antibodies directed to possible mismatches in less ideal donors is related with delay in the platelets and neutrophil engraftment and even with graft rejection. The HCT donor could be family member known as related donor or an unrelated donor. RELATED DONOR: Half of the HLA alleles or haplotype are inherited from the mother and half from the father, so each recipients sibling who has the same parents has a 25% chance of matching. There is less chances to find a matched donor in the extended family members, however, in cases where the recipient carry a rare allele or haplotype in combination with a frequent haplotype is recommended extend the ...
Can anybody please give me an up-to-date list of all the alleles of the following HLA specificities: HLA-A1, A2, A3, A11, A24, B7, B8, B35 and B44. In addition, could you also tell me the frequencies of the alleles in the population? Id be most grateful if anybody could help me on this ...
Moving back to solid science, one of the major challenges when it comes to transplantation is rejection. This is when the immune system identifies the transplant as foreign tissue and launches an assault on it, causing damage that can end in the death of the organ, and therefore possibly the recipient. Rejection is more likely when the donor and recipient are a poor tissue match. This matching is largely based on a set of proteins called Human Leukocyte Antigens (HLAs) that are found on the surface of every single cell in our bodies. Our HLAs are like a barcode that identify our cells as our own. A transplanted organ is exceptionally unlikely to be a perfect match, so its HLAs are different from our own cells. If they are too different, the immune system successfully plays spot-the-difference and targets the transplant for destruction. We can try and mitigate this by using drugs that suppress the immune system, but this immunosuppression puts the recipient at higher risk of developing ...
Example: Human leukocyte antigen (HLA) alleles[edit]. HLA constitutes a group of cell surface antigens also known as the MHC of ... Table 2. Linkage disequilibrium among HLA alleles in pan-Europeans[15] HLA-A alleles i HLA-B alleles j Δ. i. j. {\displaystyle ... Antigen i +. {\displaystyle +}. A. 1. +. {\displaystyle A1^{+}}. a. =. 376. {\displaystyle a=376}. b. =. 237. {\displaystyle b= ... HLA alleles B. 27. +. {\displaystyle B27^{+}}. a. =. 96. {\displaystyle a=96}. b. =. 77. {\displaystyle b=77}. C. {\ ...
"HLA Antigens in Kawasaki Disease". Pediatrics. 61 (2): 252-255. ISSN 0031-4005. Shigematsu, I; Shibata, S; Tamashiro, H; ...
2009). "Human embryonic stem cells hemangioblast express HLA-antigens". J Transl Med. 7 (1): 27. doi:10.1186/1479-5876-7-27. ...
"Human Leukocyte Antigen (HLA) System - Immunology; Allergic Disorders". Merck Manuals Professional Edition. Merck & Co. ... In humans MHC is also called human leukocyte antigen (HLA). Though cytotoxic-crossmatch assay can predict rejection mediated by ... Unlike virtually all other mammals, humans and other primates do not make αGal, and in fact recognize it as an antigen. During ... The T cells can recognize the donated organ's MHC antigens through one of two routes: a direct pathway, where the transplanted ...
Human leucocyte antigen polymorphisms[edit]. Main article: Human leukocyte antigen. Human leucocyte antigen (HLA) polymorphisms ... In West Africa an HLA class I antigen (HLA Bw53) and an HLA class II haplotype (DRB1*13OZ-DQB1*0501) are independently ... Gerbich antigen receptor negativity[edit]. Main article: Gerbich antigen system. The Gerbich antigen system is an integral ... Non-expression of Duffy antigen on red cells Miller, et al. 1976 P. vivax Non-expression of Duffy antigen on red cells Miller ...
"Nixon, Douglas". Makgoba, M. W. (1983). Studies on the polymorphism of HLA class II antigens (Thesis). Thesis DPhil--University ... "Common West African HLA antigens are associated with protection from severe malaria". Nature. 352 (6336): 595-600. Bibcode: ... McMichael, A. J.; Ting, A.; Zweerink, H. J.; Askonas, Brigitte A. (1977). "HLA restriction of cell-mediated lysis of influenza ... Barouch, Dan H. (1995). Peptide binding and presentation by HLA-A2 (Thesis). Thesis DPhil--University of Oxford. "Immunology ...
Presence of the HLA-B27 antigen; (3) Onset of arthritis in a male over 6 years of age; (4) Acute (symptomatic) anterior uveitis ... The Anti-nuclear antigen (ANA) is positive in up to 80% of patients with oligoarthritis and is associated with a higher risk of ... HLA-B27, Rheumatoid factor and Anti-citrullinated protein antibody. These serological markers may be negative in children with ...
CD74 (англ. HLA class II histocompatibility antigen gamma chain; HLA-DR antigens-associated invariant chain) - мембранный белок ... Riberdy J.M., Newcomb J.R., Surman M.J., Barbosa J.A., Cresswell P. HLA-DR molecules from an antigen-processing mutant cell ... Machamer C.E., Cresswell P. Biosynthesis and glycosylation of the invariant chain associated with HLA-DR antigens (англ.) // ... Ia-associated invariant chainMHC HLA-DR gamma chainCD74HLA class II histocompatibility antigen gamma chaingamma chain of class ...
Thus, the ability to generate platelet products ex vivo and platelet products lacking HLA antigens in serum-free media would ... Generated platelets demonstrated an 85% reduction in class I HLA antigens. These platelets appeared to have normal function in ... "iPSC-Derived Platelets Depleted of HLA Class I Are Inert to Anti-HLA Class I and Natural Killer Cell Immunity". Stem cell ... DC-like antigen-presenting cells obtained from human induced pluripotent stem cells can serve as a source for vaccination ...
Low levels of human leukocyte antigen (HLA-DR) make MSC's hypoimmunogenic. MSC's have trilineage differentiation where they are ...
During tissue typing, an individual's human leukocyte antigens (HLA) are identified. HLA molecules are presented on the surface ... including HLA Class I A, B, and C genes, as well as HLA Class II DRB1, DRB3, DRB4, DRB5, DQA1, DQB1, DPA1, and DPB1 genes. HLA ... If HLA from the donor is recognized by the recipient's immune system as different from the recipient's own HLA, an immune ... More specifically, HLA mismatches between organ donors and recipients can lead to the development of anti-HLA donor-specific ...
In humans MHC is also called human leukocyte antigen (HLA). Though cytotoxic-crossmatch assay can predict rejection mediated by ... Unlike virtually all other mammals, humans and other primates do not make αGal, and in fact recognize it as an antigen.[12] ... An animal's exposure to the antigens of a different member of the same or similar species is allostimulation, and the tissue is ... In the living donor, such presentation of self antigens helped maintain self tolerance.) Thereupon, the T cell receptors (TCRs ...
Among immune-related refractoriness, antibodies against HLA antigens are the primary cause. Non-immune causes include ... testing for HLA antibodies). If an immune cause is suspected and HLA antibodies are detected, then HLA-selected platelet ... HLA and HPA-selected components should not be used if no HLA or HPA antibodies are detected. Colman, Robert W.; Marder, Victor ... platelet refractoriness Pathogen-reduced platelet component Alloantibodies to platelet antigens Human leucocyte antigen (HLA) ...
Krangel MS, Orr HT, Strominger JL (December 1979). "Assembly and maturation of HLA-A and HLA-B antigens in vivo". Cell. 18 (4 ... Wang Z, Cao Y, Albino AP, Zeff RA, Houghton A, Ferrone S (February 1993). "Lack of HLA class I antigen expression by melanoma ... Saper MA, Bjorkman PJ, Wiley DC (May 1991). "Refined structure of the human histocompatibility antigen HLA-A2 at 2.6 A ... The affinity of different H-2 and HLA antigens for beta-2-microglobulin". Journal of Immunology. 140 (7): 2322-9. PMID 2450918 ...
In West Africa an HLA class I antigen (HLA Bw53) and an HLA class II haplotype (DRB1*13OZ-DQB1*0501) are independently ... Human leucocyte antigen (HLA) polymorphisms common in West Africans but rare in other racial groups, are associated with ... Polymorphisms at the HLA loci, which encode proteins that participate in antigen presentation, influence the course of malaria ... There are four alleles of the gene which encodes the antigen, Ge-1 to Ge-4. Three types of Ge antigen negativity are known: Ge- ...
"Distribution of HLA antigens in Sadhu Chetty of Tamil Nadu, South India". Anthropologischer Anzeiger. 53: 221-230 - via JSTOR. ...
"Common West African HLA antigens are associated with protection from severe malaria". Nature. 352 (6336): 595-600. doi:10.1038/ ...
The usual route for sensitisation towards HLA antigens occurs in three instances; pregnancy, post blood transfusion and prior ... which bind to HLA and/or non-HLA molecules on the endothelium. Preformed donor-specific HLA antibodies resulting in hyperacute ... It became clear with time that it did not identify all preexisting donor-specific HLA antibodies (HLA-DSA). In recent years, ... HLA frequencies and racial differences need to be factored in but cannot be done. Moreover, significant false positive results ...
June 2018). "Defective HLA class I antigen processing machinery in cancer". Cancer Immunology, Immunotherapy. 67 (6): 999-1009 ... These tumor antigens are either TSA (Tumor-specific antigen) or TAA (Tumor-associated antigen). Tumor-specific antigens (TSA) ... Oncofetal antigens are tumor-associated antigens expressed by embryonic cells and by tumors. Examples of oncofetal antigens are ... Tumor cells have non-classical MHC class I on their surface, for example HLA-G. HLA-G is inducer of Treg, MDSC, polarise ...
"SCID due to absent class II HLA antigens (Concept Id: C0242583) - MedGen - NCBI". www.ncbi.nlm.nih.gov. Retrieved 2017-07-28. ... MHC II proteins are also important for positive and negative selection in the thymus because they present antigens to immature ... MHC II proteins present exogenous antigens that activate CD4+ T-lymphocytes, immune cells that are responsible for activating ... "Orphanet: Immunodeficiency by defective expression of HLA class 2". www.orpha.net. Retrieved 2017-07-28. Bratzler, Dale W.; ...
Pöllänen P, Niemi M (1987). "Immunohistochemical identification of macrophages, lymphoid cells and HLA antigens in the human ... They are often found within tissues where they can be activated by antigen-presenting cells upon infection. They are present in ... Pöllänen P, Maddocks S (1988). "Macrophages, lymphocytes and MHC II antigen in the ram and the rat testis". Journal of ... Macrophages are directly involved in the fight against invading micro-organisms as well as being antigen-presenting cells which ...
Saper MA, Bjorkman PJ, Wiley DC (1991). "Refined structure of the human histocompatibility antigen HLA-A2 at 2.6 A resolution ... the HLA-A2 human histocompatibility antigen. This work was published in 1987, first at 3.5Å resolution (PDB entry 1HLA) and ... "Structure of the human class I histocompatibility antigen, HLA-A2". Nature. 329 (6139): 506-512. Bibcode:1987Natur.329..506B. ... "Pamela Bjorkman, PhD - ASHI 2017". 2017.ashi-hla.org. Archived from the original on 2017-11-07. Retrieved 2017-10-30. "AAI-BD ...
HLA) allo-antibodies (which are expressed by platelets) to fetuses whose platelets express the corresponding antigens. NAIT ... Platelet antigens are inherited from both mother and father. NAIT is caused by antibodies specific for platelet antigens ... "HLA-DRw52a is involved in alloimmunization against PL-A1 antigen". Human Immunology. 27 (2): 73-9. doi:10.1016/0198-8859(90) ... is strongly associated with both HLA-DRB3*0101 and HLA-DQB1*0201". Human Immunology. 34 (2): 107-14. doi:10.1016/0198-8859(92) ...
Multiple human leukocyte antigen (HLA) alleles are associated with NMOSD. NMO was associated in the past with many systemic ...
Human leukocyte antigen (HLA)-DR is positive in most patients. Occasional cases require in situ hybridization to identify the ... but immunophenotyping demonstrates myeloid antigens. In acute myeloblastic leukemia (M0), the blasts are agranular and ...
Macrophages and lymphocytes show marked expression of HLA-DR antigen. Arguably XO is the bone localization of the ...
Macrophages and T lymphocytes demonstrated a marked expression of HLA-DR antigen. A delayed type hypersensitivity reaction of ...
"P52 Characteristics of HLA Class I and Class II Antigens of the Somali Population". Transfusion Medicine. 16 (Supplement s1): ...
Altered expression of HLA class I antigens in breast cancer: association with prognosis. Int J Cancer (Pred Oncol), 89, 500-505 ...
"HLA Antigen-Sharing in Couples with Repeated Spontaneous Abortions and the Birthweight of Babies in Successful Pregnancies." ... Human body odor has been associated with the human leukocyte antigens (HLA) genomic region. They discovered that females were ... of HLA alleles theoretically giving them a wider diversity of antigens present on the surface of cells compared to HLA- ... HLA genes are highly polymorphic between individuals. Any two individuals with similar HLA genes could be possibly related. ...
Surface antigens[edit]. Terminally differentiated plasma cells express relatively few surface antigens, and do not express ... Another important surface antigen is CD319 (SLAMF7). This antigen is expressed at high levels on normal human plasma cells. It ... After leaving the bone marrow, the B cell acts as an antigen presenting cell (APC) and internalizes offending antigens, which ... cannot act as antigen-presenting cells because they no longer display MHC-II, and do not take up antigen because they no longer ...
... 和HLA-DR (人类T细胞的特异标志)。CTLA-4在活化T细胞表面的上调,对共激活受体有竞争性抑制作用,可以避免活化T细胞的过度活化。活化T细胞的表面糖基化情况也有改变[32]。 ... MR1 antigen presentation to mucosal-associated invariant T cells was highly conserved in evolution. Proceedings of the National ... An induced rebinding model of antigen discrimination. Trends Immunol. 2014, 35 (4): 153-8.
... for human leukocyte antigen (HLA) matching (see PGD for HLA matching) in order to donate to an ill sibling requiring HSCT. ... HLA-A, HLA-B, or HLA-C) increase the risk of graft rejection. A mismatch of an HLA Type II gene (i.e. HLA-DR, or HLA-DQB1) ... A compatible donor is found by doing additional HLA-testing from the blood of potential donors. The HLA genes fall in two ... the donor should preferably have the same human leukocyte antigens (HLA) as the recipient. About 25 to 30 percent of allogeneic ...
"Correlation between acetylcholine receptor antibody titer and HLA-B8 and HLA-DRw3 antigens in myasthenia gravis". Trans Am ... November 1979). "Primary biliary cirrhosis associated with HLA-DRw3". Tissue Antigens. 14 (5): 449-52. doi:10.1111/j.1399- ... Prior to refined typing for HLA-DQ and DR, the association with HLA-A1 and B8 was identified for coeliac disease in 1973 and ... HLA A1-B8-DR3-DQ2 haplotype (Also: AH8.1, COX,[1] Super B8, ancestral MHC 8.1[2] or 8.1 ancestral haplotype[3]) is a multigene ...
... human leukocyte antigens (HLA) - human papilloma virus (HPV) - human T cell lymphotropic virus type I (HTLV-I) - human T cell ... antigen - antigen presentation - antigen-presenting cell (APC) - antineoplastic - antiprotozoal - antiretroviral drugs - ... HLA - Hodgkin's disease - holistic medicine - homology (biology) - hormone - host - host factors - HPTN - HPV - HRSA - HTLV-I ...
These cells bind antigens presented on MHC I complex of virus-infected or tumour cells and kill them. Nearly all nucleated ... Basophils are chiefly responsible for allergic and antigen response by releasing the chemical histamine causing the dilation of ... Dendritic cells (Although these will often migrate to local lymph nodes upon ingesting antigens) ... class II molecules on antigen-presenting cells. Helper T cells make cytokines and perform other functions that help coordinate ...
This is carried out by using donor-derived antigen-presenting cells. These new methods have reduced culture time to 10-12 days ... HLA-DR, CD25, CD80 (B cells). Tests for T cell function: skin tests for delayed-type hypersensitivity, cell responses to ... recurrent infections and failure of the development of antibodies on exposure to antigens. The 1999 criteria also distinguish ... selective immunoglobulin A deficiency Specific antibody deficiency to specific antigens with normal B cell and normal Ig ...
... that use recombinant antigens will not have a false-positive result. ... Genetic markers: HLA-B8, HLA-DR2, HLA-DR3. *Race: Blacks, Hispanics, Asians, and Native Americans ...
... or using human leukocyte antigen antigens. The current techniques for paternity testing are using polymerase chain reaction ( ... In the 1960s, highly accurate genetic paternity testing became a possibility when HLA typing was developed, which compares the ... genetic fingerprints on white blood cells between the child and alleged parent.[10] HLA tests could be done with 80% accuracy, ...
TI-1 antigen[edit]. TI-1 antigens have an intrinsic B cell activating activity, that can directly cause proliferation and ... TI-2 antigen[edit]. Second group of TI antigens consists mainly of highly repetitive surface structures (epitopes) of ... TI-1 antigen, which has an activity that can directly activate B cells and TI-2 antigen, which has highly repetitive structure ... TI-1 antigens activate B-cells via Toll like receptors, which are, in human, expressed on the surface of B lymphocytes after ...
Antigens can be classified according to their source. Exogenous antigens[edit]. Exogenous antigens are antigens that have ... T-independent antigen - Antigens that stimulate B cells directly.. *Immunodominant antigens - Antigens that dominate (over all ... Tumor antigens[edit]. Tumor antigens are those antigens that are presented by MHC class I or MHC class II molecules on the ... A native antigen is an antigen that is not yet processed by an APC to smaller parts. T cells cannot bind native antigens, but ...
Memorijske T ćelije su podskup antigen - specifičnih T ćelijs koje traju dugoročno nakon savladavanja infekcije.[1] One se brzo ... Somatska hipermutacija • V(D)J rekombinacija • Sastavna raznovrsnost • Promena imunoglobulinske klase • MHC/HLA ... Ove ćelije prepoznaju svoje ciljeve putem vezanja za antigen koji je asociran sa molekulama MHC klase I, koje se ispoljavaju na ... MR1 antigen presentation to mucosal-associated invariant T cells was highly conserved in evolution. 2009 ...
Binding of antigens to IgE already bound by the FcεRI on mast cells causes cross-linking of the bound IgE and the aggregation ... FcεRI is expressed on mast cells, basophils, and the antigen-presenting dendritic cells in both mice and humans. ... IgE also plays a pivotal role in responses to allergens, such as: anaphylactic drugs, bee stings, and antigen preparations used ... Degranulation processes 1 - antigen; 2 - IgE antibody; 3 - FcεRI receptor; 4 - preformed mediators (histamine, proteases, ...
HLA-DR3 and HLA-DRw52 human leukocyte antigen (HLA) markers; collectively known as Jo-1 syndrome.[25][35] ... Extractable nuclear antigens[edit]. Extractable nuclear antigens (ENA) are a group of autoantigens that were originally ... Each well of a microtitre plate is coated with either a single antigen or multiple antigens to detect specific antibodies or to ... Target antigen. Sensitivity (%) SLE. Drug-induced LE. Diffuse systemic sclerosis. Limited systemic scleroderma. Sjögren ...
Exogenous antigens for IgA have not been identified in the kidney, but it is possible that this antigen has been cleared before ... Some HLA alleles have been suspected along with complement phenotypes as being genetic factors. Non-aggressive Berger's disease ... Associations described include those with C4 null allele, factor B Bf alleles, MHC antigens and IgA isotypes. ACE gene ... It has also been proposed that IgA itself may be the antigen. ... abnormal mucosal antigen handling) and not the ultimate cause ...
HLA antigen.jpg 1.361 × 876; 124 KB. *. HLA region.jpg 1.104 × 653; 52 KB. ...
... may be caused by a bacterial antigen; the occurrence of this syndrome is strongly linked to HLA-B27 genotype, but the ...
In these cases, patients should be tested for the presence of HLA-DQ2/DQ8 genetic markers because a negative HLA-DQ2 and HLA- ... There is evidence that not only gliadin (main cytotoxic antigen of gluten), but also other proteins present in gluten and ... A 2015 systematic review showed that 20% of NCGS patients who presented with negative serology, HLA-DQ2 and/or HLA-DQ8 ... especially in patients positive for HLA DQ2 and/or DQ8 haplotypes, is celiac disease, with a documented prevalences ranging ...
... they are professional antigen-presenting cells, they regulate other immune cell functions (e.g., CD4+ T cell, dendritic cell, B ...
The HLA class II locus makes patients susceptible to the condition. Most SPS patients have the DQB1* 0201 allele.[2] This ... The antibodies appear to interact with antigens in the brain neurons and the spinal cord synapses, causing a functional ...
... any antibody produced against this antigen (which mimics the self-antigens) can also, in theory, bind to the host antigens, and ... Klein J, Sato A (September 2000). "The HLA system. Second of two parts". N. Engl. J. Med. 343 (11): 782-6. doi:10.1056/ ... Molecular Mimicry - An exogenous antigen may share structural similarities with certain host antigens; thus, ... T-Cell-B-Cell discordance - A normal immune response is assumed to involve B and T cell responses to the same antigen, even if ...
"Colonia Tovar: the history of a semi-isolated Venezuelan population of German ancestry described by HLA Class I genes". Tissue ... Antigens. 62 (5): 401-407. ISSN 0001-2815.. .mw-parser-output cite.citation{font-style:inherit}.mw-parser-output .citation q{ ...
It is most commonly caused by antibodies directed against donor leukocytes and HLA antigens. This is in contrast to transfusion ... in which the donor plasma has antibodies directed against the recipient HLA antigens, mediating the characteristic lung damage ...
Antigens presented on MHC 1 molecules activates CD8+ T cells on keratinocytes or by encounters with activated CD4+ helper T ... It is associated with HLA-DQB1.[16][36] ... Autoimmune response to epithelial self-antigens remains a ... An immune-mediated mechanism where basal keratinocytes are being targeted as foreign antigens by activated T cells, especially ...
HLA-DQ is part of the MHC class II antigen-presenting receptor (also called the human leukocyte antigen) system and ... The two subunits of the HLA-DQ protein are encoded by the HLA-DQA1 and HLA-DQB1 genes, located on the short arm of the sixth ... Almost all people (95%) with coeliac disease have either the variant HLA-DQ2 allele or (less commonly) the HLA-DQ8 allele.[28][ ... Furthermore, around 5% of those people who do develop coeliac disease do not have typical HLA-DQ2 or HLA-DQ8 alleles (see below ...
... mobilized human leukocyte antigen (HLA)-mismatched allogeneic peripheral blood stem cells following a reduced-intensity ... Matched HLA between donor and recipient is not necessary. The stem cells are collected from donor's blood through a process ... Donor's Requirements: Mismatched HLA with recipient Age between 18 and 60 years old Good overall state of health No major heart ... To overcome the intolerable severe reactions of high-dose chemotherapy and GVHD, as well as the challenge to find HLA-matched ...
B51 is a split antigen of the broad antigen B5, and is a sister serotype of B52.[2] There are a large number of alleles within ... derived from IMGT/HLA *^ a b c d e f g h i j k l m n o p q r s t u v w x y z aa ab ac ad ae af ag ah ai aj ak al am an ao ap aq ... EBI-HLA B*5101 1e28​, 1e27​ HLA-B51 (B51) is an HLA-B serotype. The serotype identifies the more common HLA-B*51 gene products. ... the association of HLA antigens". J. Med. Virol. 7 (4): 287-97. doi:10.1002/jmv.1890070405. PMID 6950026.. ...
HLA-B12, HLA-B51, HLA-Cw7, HLA-A2, HLA-A11, and HLA-DR2 are examples of human leukocyte antigen types associated with aphthous ... or present a more substantial barrier to microbes and antigens, but this is unclear. Nicotine is also known to stimulate ... stomatitis.[2][5] However, these HLA types are inconsistently associated with the condition, and also vary according to ...
... is part of a family of genes called the human leukocyte antigen (HLA) complex. The HLA complex helps the immune system ... HLA-B*45ZJ, HLA-B-3506, HLA-B-3905, HLA-B-5502, HLA-B-5602, HLA-B15, HLA-B39, HLA-B49, HLA-B50, HLA-B55, HLA-B59, HLA-B61, HLA- ... They are HLA-A, HLA-B, (both Class I MHCs) and HLA-DR (a Class II MHC).[5] If the two tissues have the same genes coding for ... In humans, the HLA-B gene and two related genes, HLA-A and HLA-C, are the major genes in MHC class I. ...
HLA)). This MHC: antigen complex is recognized by T-cells passing through the lymph node. Exogenous antigens are usually ... Exogenous antigensEdit. Antigen presentation stimulates T cells to become either "cytotoxic" CD8+ cells or "helper" CD4+ cells. ... Antigen presentationEdit. Main article: Antigen presentation. Acquired immunity relies on the capacity of immune cells to ... Endogenous antigensEdit. Endogenous antigens are produced by intracellular bacteria and viruses replicating within a host cell ...
HLA) The human leukocyte antigen (HLA) is not a single antigen, but is rather a group of proteins that are located on the ... Human leukocyte antigen (HLA). The human leukocyte antigen (HLA) is not a single antigen, but is rather a group of proteins ... Research on human blood cells in the 1950s identified three genes associated with the HLA (HLA-A, HLA-B, HLA-C). In the 1970s, ... Antigen World of Forensic Science COPYRIGHT 2005 Thomson Gale. Antigen. Antigens, which are usually proteins or polysaccharides ...
AntigensHLA AntigensHLA-A AntigensHLA-A1 AntigenHLA-A11 AntigenHLA-A2 AntigenHLA-A24 AntigenHLA-A3 AntigenHLA-B AntigensHLA-B13 ... AntigensHLA AntigensHLA-A AntigensHLA-A1 AntigenHLA-A11 AntigenHLA-A2 AntigenHLA-A24 AntigenHLA-A3 AntigenHLA-B AntigensHLA-B13 ... AntigenHLA-B39 AntigenHLA-B40 AntigenHLA-B44 AntigenHLA-B51 AntigenHLA-B52 AntigenHLA-B7 AntigenHLA-B8 AntigenHLA-C AntigensHLA ... AntigenHLA-B39 AntigenHLA-B40 AntigenHLA-B44 AntigenHLA-B51 AntigenHLA-B52 AntigenHLA-B7 AntigenHLA-B8 AntigenHLA-C AntigensHLA ...
... antigen-presenting molecules, and other proteins involved in immune function. The human leukoc ... The earliest HLA associations with rheumatic diseases, such as the association of the HLA-B*27 allele at the HLA-B gene with ... antigen-presenting molecules, and other proteins involved in immune function. The human leukocyte antigen (HLA) complex is ... including the human leukocyte antigen (HLA) genes (figure 1). Thus, the human MHC region is also referred to as the HLA region ...
The protein is called human leukocyte antigen B27 (HLA-B27). ... HLA-B27 is a blood test to look for a protein that is found on ... Human leukocyte antigen B27; Ankylosing spondylitis-HLA; Psoriatic arthritis-HLA; Reactive arthritis-HLA ... The protein is called human leukocyte antigen B27 (HLA-B27).. Human leukocyte antigens (HLAs) are proteins that help the bodys ... Human Leukocyte Antigen (HLA) B-27 - blood. In: Chernecky CC, Berger BJ, eds. Laboratory Tests and Diagnostic Procedures. 6th ...
Typing for HL-A antigens had shown a positive correlation between HL-A 8 and myasthenia gravis which was significantly higher ... HL-A 2-positive patients more often had thymomas and antibodies to skeletal muscle than HL-A 2-negative patients; HL-A 2- ... The fact that the clinical aspects of the HL-A 8-negative and HL-A 2-positive patients were different from those of the HL-A 8- ... Myasthenia Gravis, Autoantibodies, and HL-A Antigens Br Med J 1974; 1 :131 ...
A process called HLA typing makes sure that the donor and recipient are closely matched. ... Why HLA testing is done. HLA testing is done to identify your pattern of antigens and to find antibodies to the HLA antigens. ... Human leukocyte antigen (HLA) testing. Human leukocyte antigen (HLA) testing is also called HLA typing or tissue typing. It is ... How HLA testing is done. A sample of blood is taken by inserting a needle into a vein in your arm. Sometimes a swab of cells is ...
A process called HLA typing makes sure that the donor and recipient are closely matched. ... Why HLA testing is done. HLA testing is done to identify your pattern of antigens and to find antibodies to the HLA antigens. ... Human leukocyte antigen (HLA) testing. Human leukocyte antigen (HLA) testing is also called HLA typing or tissue typing. It is ... Cancer information / Diagnosis and treatment / Tests and procedures / Human Leukocyte Antigen (HLA) testing ...
... neural network for predicting the likelihood of antigen presentation from a gene of interest in the context of specific HLA ... In addition to in vitro binding measurements, MARIA is trained on peptide HLA ligand sequences identified by mass spectrometry ... A neural network trained on diverse datasets improves prediction of HLA class II epitope presentation. ... expression levels of antigen genes and protease cleavage signatures. Because it leverages these diverse training data and our ...
A test indicating that HLA-B27 is present means that the patient might have a risk of... ... HLA-B27 is a human leukocyte antigen that helps the body differentiate its own cells from foreign substances. ... Patients may get an HLA-B27 antigen test to find the cause of joint swelling and pain. Another reason for an HLA test is to see ... HLA-B27 is a human leukocyte antigen that helps the body differentiate its own cells from foreign substances. A test indicating ...
Human leukocyte antigen (HLA) typing. What is this test?. This test looks at the human leukocyte antigens (HLA) in your blood. ... Testing helps make sure you have the best possible match between your HLA antigens and those on the organ you receive. You may ... The results will show the degree to which HLA antigens match between you and the donor. ... HLA Antigen. Does this test have other names?. ... antibodies that will react with HLA antigens on a new ...
Human leukocyte antigens (HLA) associated drug hypersensitivity: consequences of drug binding to HLA.. Yun J1, Adam J, Yerly D ... As HLA molecules are a critical element in T-cell stimulation, it is no surprise that particular HLA alleles have a direct ... Recent publications have shown that certain human leukocyte antigen (HLA) alleles are strongly associated with hypersensitivity ... In some HLA-associated drug hypersensitivity reactions, the presence of a risk allele is a necessary but incomplete factor for ...
HLA B17, B37 and B62 were significantly increased in PsA patients. Univariate analyses suggest that the HLA antigens B37, B62 ... clinical manifestations were more reliable predictors of aggressive joint damage than were specific HLA antigens. However, HLA ... Disease manifestations and HLA antigens in psoriatic arthritis in northern Sweden. Alenius, Gerd-Marie Umeå universitet, ... HLA antigens, Joint damage, Psoriatic arthritis Nationell ämneskategori Reumatologi och inflammation Identifikatorer. URN: urn: ...
Antigens, HLA-DS. A group of the D-related HLA Antigens found to differ from the DR Antigens in Genetic Locus and therefore ... These Antigens are polymorphic Glycoproteins comprising alpha and beta chains and are found on lymphoid and other Cells, often ...
Antigens, HLA B. Class I Human Histocompatibility (HLA) Surface Antigens encoded by more than 30 detectable Alleles on locus B ... of the HLA complex, the most polymorphic of all the HLA specificities. Several of these Antigens (e.g., HLA-B27, -B7, -B8) are ... Like other class I HLA determinants, they are involved in the cellular immune reactivity of cytolytic T Lymphocytes. ...
HLA class II regulation and structure: Analysis with HLA-DR3 and HLA-DP point mutants. J Exp Med 1985; 162: 1193-1207.CrossRef ... Antigen presentation and assembly by mouse I-Ak class II molecules in human APC containing deleted or mutated HLA DM molecules ... Accumulation of HLA-DM, a regulator of antigen presentation, in MHC class II compartments. Science 1994; 266: 1566-1569. ... Invariant chain peptides in most HLA-DR molecules of an antigen-processing mutant. Science 1992; 258: 1801-1804.CrossRefGoogle ...
Recent evidence suggests that reduced expression of target protein antigens and human leukocyte antigen (HLA) molecules is the ... Recent evidence suggests that reduced expression of target protein antigens and human leukocyte antigen (HLA) molecules is the ... HLA class II antigen presentation by prostate cancer cells Prostate Cancer Prostatic Dis. 2008;11(4):334-41. doi: 10.1038/sj. ... The purpose of this study was to investigate the prospect of antigen specific immunotherapy against prostate cancer via the HLA ...
Epitope analysis of HLA-DQ antigens: what does the antibody see?. [Anat R Tambur, Jimmy Rosati, Shirley Roitberg, Denis Glotz, ... Human leukocyte antigen (HLA)-DQ has emerged as the alloantibody most frequently associated with the generation of de novo ... Our data support the need for changing the manner in which HLA-DQ antigens and antibodies are evaluated for organ ... The generation of HLA-DQ de novo DSA was interrogated in 40 transplant recipients who were immunologically naive before their ...
Hla-a antigens explanation free. What is Hla-a antigens? Meaning of Hla-a antigens medical term. What does Hla-a antigens mean? ... Looking for online definition of Hla-a antigens in the Medical Dictionary? ... redirected from Hla-a antigens). Also found in: Dictionary, Encyclopedia. human leukocyte antigen (HLA). any one of four ... human leukocyte antigen (HLA). The human MAJOR HISTOCOMPATIBILITY COMPLEX.. human leukocyte antigen. ; HLA antigenic molecules ...
... are presented on the cell surface in the context of HLA-A*02:01 (HLA-A2) molecules (13, 14). HLA-A2 is the most common HLA-I ... Pr20 binds to ALY/HLA-A2 complexes in PRAME/HLA-A2-expressing leukemias. TCRm clones reactive with ALY/HLA-A2 complexes were ... HLA-A2- AML cell line HL60, indicating that the epitope was restricted by HLA-A2. In addition, Pr20 did not bind PRAME-HLA-A2+ ... We aimed to identify a TCRm Ab that recognized ALY/HLA-A2, but not HLA-A2 alone or in complex with irrelevant HLA-A2-binding ...
A therapeutic T cell receptor mimic antibody targets tumor-associated PRAME peptide/HLA-I antigens. ... A therapeutic T cell receptor mimic antibody targets tumor-associated PRAME peptide/HLA-I antigens. ... Preferentially expressed antigen in melanoma (PRAME) is a cancer-testis antigen that is expressed in many cancers and leukemias ... is presented in the context of human leukocyte antigen HLA-A*02:01 molecules for recognition by the T cell receptor (TCR) of ...
tr,Q30003,Q30003_HUMAN Lymphocyte antigen (Fragment) OS=Homo sapiens OX=9606 GN=HLA-DQB1 PE=4 SV=1 ... Lymphocyte antigenImported. ,p>Information which has been imported from another database using automatic procedures.,/p> ,p>,a ...
HLA class I typing, low resolution one. Antigen equivalent. Service Code: 81374, Service Type: Medical ...
HLA) in your blood. It helps match donors and recipients for stem-cell and organ transplants. ... This test looks at the human leukocyte antigens ( ... HLA Antigen. Does this test have other names?. Human leukocyte ... antigen (HLA) typing. What is this test?. This test looks at the human leukocyte antigens (HLA) in your blood. This test is ... Testing helps make sure you have the best possible match between your HLA antigens and those on the organ you receive. You may ...
If patients could recognise themselves, or anyone else could recognise a patient from your description, please obtain the patients written consent to publication and send them to the editorial office before submitting your response [Patient consent forms] ...
HLA class I and II antigens are partially co-clustered in the plasma membrane of human lymphoblastoid cells. Attila Jenei, ... HLA class I and II antigens are partially co-clustered in the plasma membrane of human lymphoblastoid cells ... Alteration of antigen-independent immunologic synapse formation between dendritic cells from HLA-B27-transgenic rats and CD4+ T ... HLA class I and II antigens are partially co-clustered in the plasma membrane of human lymphoblastoid cells ...
HLA Antigens in Spanish Patients with Essential Hypertension. A. Fernandez-Cruz Jr, M. Luque Otero, L. Llorente Perez, C. ... HLA Antigens in Spanish Patients with Essential Hypertension. A. Fernandez-Cruz, M. Luque Otero, L. Llorente Perez, C. ... HLA Antigens in Spanish Patients with Essential Hypertension Message Subject (Your Name) has forwarded a page to you from ... 5. In view of a previous report of HLA antigens in a Spanish diabetic population, this study does not support the suggestion of ...
Genetic modulation of antigen presentation by HLA-B27 molecules.. L Pazmany, S Rowland-Jones, S Huet, A Hill, J Sutton, R ... Genetic modulation of antigen presentation by HLA-B27 molecules.. L Pazmany, S Rowland-Jones, S Huet, A Hill, J Sutton, R ... These data are compatible with the presence of a factor(s), possibly HLA linked, interfering with antigen presentation by ... None of the eight B cell lines that expressed HLA-B27 presented a known peptide epitope to CTL. However, cells from a family ...
HLA-DR antigens in small and large intestinal epithelia were examined in Crohns disease (CD). Seventy-two biopsy specimens (10 ... Crohns disease elemental diet HLA-DR antigens large intestine small intestine This work was partly supported by a grant from ... Wang CY, Al-Katib A, Lane CL, et al: Induction of HLA-DC/ DS (Leu 10) antigen expression by human precursor B cell lines. J Exp ... HLA-DR antigens in small and large intestinal epithelia were examined in Crohns disease (CD). Seventy-two biopsy specimens (10 ...
HLA) antigens encoded by a small cluster of structural genes at the C locus on chromosome 6. They have significantly lower ... immunogenicity than the HLA-A and -B determinants and are therefore of minor importance in donor/recipient crossmatching. Their ... Antigens: 114404*Surface Antigens: 4354*Histocompatibility Antigens: 1123*HLA Antigens: 2336*HLA-C Antigens: 379*HLA-Cw6: 97 ... Histocompatibility Antigens: 1123*HLA Antigens: 2336*HLA-C Antigens: 379*HLA-Cw6: 97 ...
A group of the D-related HLA antigens (human) found to differ from the DR antigens in genetic locus and therefore inheritance. ... These antigens are polymorphic glycoproteins comprising alpha and beta chains and are found on lymphoid and other cells, often ... HLA-DP; HLA-PL; HLA-SB; Antigens, HLA-DP; Antigens, HLA-PL; Antigens, HLA-SB; HLA DP Antigens; HLA PL Antigens; HLA SB Antigens ... Surface Antigens: 4354*Histocompatibility Antigens: 1123*HLA Antigens: 2336*HLA-D Antigens: 214*HLA-DP Antigens: 231*HLA-DPB1: ...
  • http://hla.alleles.org/nomenclature/ (Accessed on November 29, 2017). (uptodate.com)
  • https://maria.stanford.edu/ ), a multimodal recurrent neural network for predicting the likelihood of antigen presentation from a gene of interest in the context of specific HLA class II alleles. (nature.com)
  • Recent publications have shown that certain human leukocyte antigen (HLA) alleles are strongly associated with hypersensitivity to particular drugs. (nih.gov)
  • As HLA molecules are a critical element in T-cell stimulation, it is no surprise that particular HLA alleles have a direct functional role in the pathogenesis of drug hypersensitivity. (nih.gov)
  • Class I Human Histocompatibility (HLA) Surface Antigens encoded by more than 30 detectable Alleles on locus B of the HLA complex, the most polymorphic of all the HLA specificities. (online-medical-dictionary.org)
  • A disparity between antigen density and mean fluorescence intensity values for some alleles within an eplet group was noted, with mean fluorescence intensity values of the lowest fluorescence bead being one tenth of the highest fluorescence bead, despite the fact that the amount of antigen on these beads were not significantly different. (sigmaaldrich.com)
  • A broad specificity HLA-DR antigen that is associated with HLA-DRB1 CHAINS encoded by DRB1*01:15 and DRB1*01:16 alleles. (harvard.edu)
  • for example, there appear to be 300+ alleles in the HLA-B or DRB1 loci. (bio-medicine.org)
  • Several probes could be used to identify an array of HLA alleles. (bio-medicine.org)
  • By constructing an array of PCR primers complementary to the range of HLA polymorphisms, it is possible to detect the HLA alleles directly by PCR. (bio-medicine.org)
  • To date, two RA genetic susceptibility factors have been identified: HLA-DRB1-SE (Shared epitope) and PTPN22 620W alleles. (bioportfolio.com)
  • The predictive value of the alleles for diagnosis of RA was previously investigated in cohorts of caucasians patients with early unclassified arthritis that showed restrained association between RA and HLA-SE. (clinicaltrials.gov)
  • The association between the different HLA alleles among French West-Indian RA patients and the autoantibodies production. (clinicaltrials.gov)
  • There were no associations between HLA DRB1 alleles and clinical characteristics of CLL patients at diagnosis, including age, clinical stage according to Rai classification, surface CD38 expression, serum levels of lactate dehydrogenase (LDH) and β2-microglobulin ( data not shown ). (haematologica.org)
  • Neither of assessed HLA DRB1 alleles was associated with response to first-line treatment or mortality. (haematologica.org)
  • Clinical stage according to Rai classification as a unique parameter (Rai 0-1 vs 2-4), with HLA DRB1* 01, HLA DRB1*02 null alleles and CD38 surface expression was included for analysis. (haematologica.org)
  • Because of their invol- vement in generating immune responses, Human Leukocyte Antigen (HLA) alleles are considered can- didate genetic risk markers for periodontitis. (scirp.org)
  • Results: HLA-A*30 (P-value = 0.010), HLA- B*41 (P1 = 0.012 and P2 = 0.014), HLA-DRB1*13 (P1 = 0.031 and P2 = 0.063) alleles seemed to be associ- ated with protection against AP in Lebanese patients. (scirp.org)
  • Conclusion: In conclusion, protective, but no sus- ceptible HLA alleles were detected in AP. (scirp.org)
  • HLA -B*18 and HLA -Cw*07 alleles was found more frequently in Group1 than in Group 2 (14.3 percent vs 2.7 percent, p=0.026, and 31 percent vs 14.9 percent, p=0.036, respectively). (aaem.pl)
  • However, the frequencies of HLA -A*03 and HLA -Cw*03 alleles were increased in Group 2 compared to Group 1 (20.3 percent vs 7.1 percent, p=0.049 and 10.8 percent vs 0 percent, p=0.024, respectively). (aaem.pl)
  • Among HLA -class II genotypes, HLA -DQB1*03 allele was significantly increased in Group 2 (60.9 percent vs 38.1 percent, p=0.018), while a higher frequency of HLA -DR B1*03 and HLA -DR B1*14 alleles showed a tendency statistically significant in Group 1 (9.5 percent vs 1.4 percent, p=0.057 and 11.9 percent vs 2.7 percent, p=0.058, respectively). (aaem.pl)
  • HLA -B*18 and HLA -Cw*07 alleles may probably be associated with susceptibility to venom allergy, whereas HLA -A*03, HLA -Cw*03 and HLA -DQB1*03 seem to be protective markers in a small Turkish population. (aaem.pl)
  • Our panel of highly representative HLA-DQ alleles will help to drive novel research to prevent transplant rejection and to contribute to more insight into DSA responses and we are thrilled to be working with Dr. Tambur, a world leader in this field. (californianewswire.com)
  • The applicability of hTERT as a potential target for anticancer immunotherapy would be widened by the identification of epitopes restricted to other common HLA alleles, such as HLA-A3 antigen. (aacrjournals.org)
  • No statistically significant differences were observed in the distribution of HLA‐G alleles between controls and RSA couples, however, 15% of the RSA women carried the HLA‐G*0106 allele compared to 2% of the control women. (deepdyve.com)
  • Furthermore, the HLA‐G alleles without the 14 bp sequence were prominent in the RSA males in contrast to the RSA women in whom alleles including the 14 bp sequence were frequently observed, especially as homozygotes. (deepdyve.com)
  • Another hypothesis concerned certain HLA‐G alleles associated with an altered expression profile of HLA‐G isoforms or reduced expression of certain HLA‐G isoforms. (deepdyve.com)
  • EH-57.1+ individuals therefore carry a 26 times higher risk of developing type I psoriasis than individuals who are EH-57.1-negative Further analysis of individual HLA alleles revealed that within EH-57.1, HLA class I antigens (Cw6-B57) were associated to a much higher extent with type I psoriasis than the HLA class II alleles (DRB1*0701-DQA1*0201-DQB1* 0303). (diva-portal.org)
  • To identify possible epitopes presented by distinct HLA class II alleles, overlapping 18-mer peptides derived from NY-ESO-1 were synthetized and tested for recognition by CD4(+) T lymphocytes in autologous settings. (uzh.ch)
  • We identified three NY-ESO-1-derived peptides presented by DRB4*0101-0103 and recognized by CD4(+) T lymphocytes of two melanoma patients sharing these HLA class II alleles. (uzh.ch)
  • We have collected peripheral blood samples 26 patients receiving HCT and generated CTL restricted by HLA alleles commonly seen in Japanese population. (nii.ac.jp)
  • Journal Article] Identification of novel CTL epitopes of CMV-pp65 presented by a variety of HLA alleles. (nii.ac.jp)
  • in there you will find a Supplemental Table that includes a list of SNPs and alleles that can be used to predict the classical HLA alleles, as well as r2 values between SNP alleles and HLA alleles. (biostars.org)
  • Rather, class II molecules are on the surface of immune cells such as macrophages and B-lymphocytes that are designed to process cells and present the antigens from these cells to T lymphocytes. (encyclopedia.com)
  • The two classes of histocompatibility molecules allow an organism to in essence establish an inventory of what cells are "self" and to expose foreign antigens to the immune system so that antibodies to these antigens can be made. (encyclopedia.com)
  • The major histocompatibility complex (MHC) is a term used to describe a group of genes in animals and humans that encode a variety of cell surface markers, antigen-presenting molecules, and other proteins involved in immune function. (uptodate.com)
  • Accurate prediction of antigen presentation by human leukocyte antigen (HLA) class II molecules would be valuable for vaccine development and cancer immunotherapies. (nature.com)
  • An essential role for HLA-DM in antigen presentation by class II major histocompatibility molecules. (springer.com)
  • The extracellular domains of MHC class II molecules determine their processing requirements for antigen presentation. (springer.com)
  • HLA-DR molecules from an antigen-processing mutant cell line are associated with invariant chain peptides. (springer.com)
  • Invariant chain peptides in most HLA-DR molecules of an antigen-processing mutant. (springer.com)
  • Recent evidence suggests that reduced expression of target protein antigens and human leukocyte antigen (HLA) molecules is the predominant immune escape mechanism of malignant prostate tumor cells. (nih.gov)
  • Prostate tumor cells transduced with class II molecules efficiently presented tumor-associated antigens/peptides to CD4+ T cells. (nih.gov)
  • In this context, a direct interaction of the relevant drug with HLA molecules as described by the p-i concept appears to be more relevant than presentation of hapten-modified peptides. (nih.gov)
  • In contrast, abacavir generates polyclonal T-cell response by interacting specifically with HLA-B*57:01 molecules. (nih.gov)
  • In summary, drug hypersensitivity is the end result of a drug interaction with certain HLA molecules and TCRs, the sum of which determines whether the ensuing immune response is going to be harmful or not. (nih.gov)
  • Genetic modulation of antigen presentation by HLA-B27 molecules. (rupress.org)
  • In studies of antigenic peptide presentation, we have found a healthy volunteer whose lymphoblastoid cells were unable to present three different virus-derived epitopes to cytotoxic T lymphocytes (CTL) despite expressing the correct restricting HLA-B27 molecules on the cell surface. (rupress.org)
  • The cloned cDNA was transfected into HLA-A- and B-negative HMy/C1R cells, and the B2702 molecules generated in this environment rendered these cells, after incubation with peptide, susceptible to lysis by peptide-specific CTL. (rupress.org)
  • These data are compatible with the presence of a factor(s), possibly HLA linked, interfering with antigen presentation by otherwise normal B2702 molecules in this family. (rupress.org)
  • After proteasomal processing, the PRAME300-309 peptide ALYVDSLFFL (ALY) is presented in the context of human leukocyte antigen HLA-A*02:01 molecules for recognition by the T cell receptor (TCR) of cytotoxic T cells. (jci.org)
  • HLA-DM captures partially empty HLA-DR molecules for catalyzed removal of peptide. (harvard.edu)
  • Helper T cells are stimulated to fight infections or diseases upon recognition of peptides from antigens that are processed and presented by the proteins of Major Histocompatibility Complex (MHC) Class II molecules. (frontiersin.org)
  • In general, polymorphic classical MHC class II molecules bind and present peptide antigens. (frontiersin.org)
  • Exogenous antigens that have been endocytosed by the APC are thus readily available for presentation via MHC II molecules, and for this reason this antigen presentation pathway is usually referred to as exogenous. (rcsb.org)
  • To produce a MHC class II molecule that presents an antigen, three MHC class II molecules (heterodimers of an alpha and a beta chain) associate with a CD74 trimer in the ER to form a heterononamer. (rcsb.org)
  • HLA-DM stabilizes MHC class II molecules until primary high affinity antigenic peptides are bound. (rcsb.org)
  • In B-cells, the interaction between HLA-DM and MHC class II molecules is regulated by HLA-DO. (rcsb.org)
  • Lysosomal microenvironment has been implicated in the regulation of antigen loading into MHC II molecules, increased acidification produces increased proteolysis and efficient peptide loading. (rcsb.org)
  • 3 MHC class II molecules bind to a CD74 homotrimer (also known as invariant chain or HLA class II histocompatibility antigen gamma chain). (rcsb.org)
  • Class II molecules are expressed in antigen presenting cells (APC: B lymphocytes, dendritic cells, macrophages). (prospecbio.com)
  • The crucial immunological function of the classical human major histocompatibility complex (MHC) class I molecules, human histocompatibility leukocyte antigen (HLA)-A, -B, and -C, is the presentation of peptides to T cells. (rupress.org)
  • The fetus, semiallograft by its genotype, escapes maternal allorecognition by downregulation of HLA-A and HLA-B molecules at this interface. (rupress.org)
  • We present three NK lines that are inhibited via the interaction of their NKAT3 receptor with HLA-G and with HLA-Bw4 molecules. (rupress.org)
  • Class I HLA molecules have diversified into two distinct, though structurally related, families of proteins. (diabetesjournals.org)
  • Unlike class Ia HLA molecules, class Ib HLA-E, -F, and -G antigens display very limited or no polymorphism within the human population, may function in presenting limited sets of peptides, and/or exhibit a restricted tissue expression ( 2 ). (diabetesjournals.org)
  • Thus, unlike polymorphic class Ia HLA molecules, which have evolved as efficient activators of immune responses, presentation of HLA-G may have specialized to increase the activation/effector thresholds of T-, NK, and antigen-presenting cells for the immune protection of the semiallogeneic fetus and certain autologous tissues. (diabetesjournals.org)
  • Antigen presentation to T cells is mediated by antigen-presenting cells (APCs) via two classes of HLA molecules: HLA Class I, recognized by CD8 + -expressing T cells (Class I is present on nearly all nucleated cells), and HLA Class II, recognized by CD4 + -expressing T cells. (diabetesjournals.org)
  • Peptides extracted from HLA-A2.1 class I major histocompatibility complex (MHC) molecules expressed on the antigen processing mutant CEMx721.174.T2 were characterized by electrospray ionization-tandem mass spectrometry. (sciencemag.org)
  • It binds to the leader peptide derived from the polymorphic classical major histocompatibility molecules HLA-A, HLA-B and HLA-C. This peptide binding is highly specific and stabilizes the HLA-E protein, allowing it to migrate to the cell surface. (portlandpress.com)
  • The anti-tumor effect of DLI after HLA-matched alloSCT is mediated by donor T cells recognizing minor histocompatibility antigens (MiHA) in the context of HLA molecules. (haematologica.org)
  • 12 Since these MiHA were all presented in HLA-A*02:01 and B*07:02, we selected 80 third-party EBV-B cell lines for co-expression of these HLA molecules, and genotyped all cell lines for more than one million SNPs. (haematologica.org)
  • This work requires availability of purified HLA-DQ and other HLA class II molecules, maintaining high physiologic accuracy of the three-dimensional structure. (californianewswire.com)
  • Since CD4(+) T lymphocytes play a critical role in generating antigen-specific cytotoxic T lymphocyte and antibody responses, we searched for NY-ESO-1 epitopes presented by histocompatibility leukocyte antigen (HLA) class II molecules. (uzh.ch)
  • HLA-E belongs to the MHC Class I molecules (MHC Class Ib, nonclassical) and it is expressed on. (biomol.com)
  • The published results revealed that the antibody cross-reacts with some classical MHC Class I molecules (MHC Class Ia): HLA-B7 (strongly), HLA-B8 (moderately), HLA-B27, -B44 (weakly). (biomol.com)
  • Here, we isolated exosomes from K562 cells (referred to as CoEX-A2s) engineered to express human leukocyte antigen (HLA)-A2 and costimulatory molecules such as CD80, CD83, and 41BBL. (ovid.com)
  • Fig. 4: MARIA trained on human HLA-DQ ligand peptides identified celiac-related gluten antigens. (nature.com)
  • Binds peptides derived from antigens that access the endocytic route of antigen presenting cells ( APC ) and presents them on the cell surface for recognition by the CD4 T-cells. (rcsb.org)
  • MHC class II molecule interacts with HLA_DM, and HLA_DO in B-cells, in order to release CLIP and facilitate the binding of antigenic peptides. (rcsb.org)
  • HLA-DRB1 takes an essential part in the immune system by presenting peptides derived from extracellular proteins. (prospecbio.com)
  • While the high polymorphism of these proteins ensures immune recognition of a variety of self-antigens and viral peptides, it also represents a major barrier to allo-transplantation. (diabetesjournals.org)
  • These CTL clones did not recognize any of the 10 peptides selected for specific association to HLA-A2.1 and derived from Melan-A/Mart-1, tyrosinase, gp100, or MAGE-3 proteins. (jimmunol.org)
  • Class I HLAs present peptides from inside the cell whereas class II HLAs present antigens from outside of the cell to T-lymphocytes. (antibodies-online.com)
  • A recent study shows that individuals with the allele HLA-B*46:01 have the fewest predicted binding peptides for SARS-CoV-2, suggesting they may be particularly vulnerable to COVID-19, as they were previously shown to be for SARS. (antibodies-online.com)
  • A different allele, HLA-B*15:03, showed the greatest capacity to present highly conserved SARS-CoV-2 peptides that are shared among common human coronaviruses, suggesting it could enable cross-protective T-cell based immunity. (antibodies-online.com)
  • Improved induction of melanoma-reactive CTL with peptides from the melanoma antigen gp100 modified at HLA-A*0201-binding residues. (jimmunol.org)
  • Upon stimulation with these peptides, melanoma-reactive CTL could be induced in vitro from PBL of some HLA-A2+ melanoma patients. (jimmunol.org)
  • Therefore, to enhance the immunogenicity of gp100 peptides, amino acid substitutions were introduced into G9154, G9209, and G9280 at HLA-A*0201-binding anchor positions, but not at TCR contact residues, to increase peptide class I MHC-binding affinity. (jimmunol.org)
  • Several modified gp100 peptides bound with greater affinity to HLA-A*0201 than unmodified peptides and were recognized by TIL specific for the natural epitopes. (jimmunol.org)
  • These peptides were used to sensitize PBL from HLA-A2+ melanoma patients in vitro using peptide-pulsed autologous PBMC as stimulators. (jimmunol.org)
  • Only seven dominant peptides were found, in contrast to over 200 associated with HLA-A2.1 on normal cells. (sciencemag.org)
  • These peptides were derived from the signal peptide domains of normal cellular proteins, were usually larger than nine residues, and were also associated with HLA-A2.1 in normal cells. (sciencemag.org)
  • A functioning TAP (transporter associated with antigen processing) molecule is required to transport these peptides into the endoplasmic reticulum, where they can interact with HLA-E. HLA-E then migrates to the cell surface, where it interacts with CD94/NKG2A receptors on natural killer cells. (portlandpress.com)
  • In this study, we extended our search for hTERT-derived immunogenic peptides to HLA-A3 antigen because this allele is expressed by 15-25% of patients and identify one such peptide that can trigger HLA-A3-restricted CTLs that kill hTERT + tumors from multiple histologies. (aacrjournals.org)
  • Human leukocyte antigen class I (HLA-I) presents antigenic peptides to cytotoxic CD8+ T cells (CTLs). (lu.se)
  • Antigen processing machinery (APM) proteins are involved in the maturation of HLA-I and in the selection of which peptides are - or are not - presented. (lu.se)
  • Additionally, in total 663 T-cell clones (containing at least 91 unique clones expressing different T-cell receptors) directed against HLA*02:01-restricted peptides of TAA WT1-RMF, RHAMM-ILS, Proteinase-3-VLQ, PRAME-VLD and NY-eso-1-SLL were isolated from HLA-A*02:01pos donors. (onmedica.com)
  • Further, the therapeutic efficacy of active immunization using antigenic HLA class I-restricted peptides may be improved by adding HLA class II-presented epitopes. (uzh.ch)
  • Interaction of HLA-DR with an acidic face of HLA-DM disrupts sequence-dependent interactions with peptides. (semanticscholar.org)
  • Using the information of the binding motif of HLA-B35, we selected and synthesized 61 different peptides (8mer to 10 mer) from TC cysteine protease. (nii.ac.jp)
  • HLA testing is done to identify your pattern of antigens and to find antibodies to the HLA antigens. (cancer.ca)
  • HLA antibodies. (rochester.edu)
  • People who have been pregnant or get a blood transfusion or organ transplant may have antibodies that will react with HLA antigens on a new transplant. (rochester.edu)
  • Our data support the need for changing the manner in which HLA-DQ antigens and antibodies are evaluated for organ transplantation. (sigmaaldrich.com)
  • The data provide rationale for developing TCRm antibodies as therapeutic agents for cancer, offer mechanistic insight on proteasomal regulation of tumor-associated peptide/HLA antigen complexes, and yield possible therapeutic solutions to target antigens with ultra-low surface presentation. (jci.org)
  • HLA-DR antigens on the intestinal epithelia were identified by the indirect immunoperoxidase staining method using two mouse anti-HLA-DR monoclonal antibodies. (springer.com)
  • Ogasawara K, Kojima H, Ikeda H, et al: A study on class II antigens involved in the T cell proliferative responses to PPD using cross-reacting monoclonal antibodies in human and murine system. (springer.com)
  • This conclusion is based on the following observations: (i) Water-soluble octamers of viral coat proteins inhibit the complement-dependent cytotoxicity of antibodies directed against H-2K and H-2D antigens in mouse cells. (pnas.org)
  • Transplantation induces new antibodies reactive to non-HLA antigens. (asnjournals.org)
  • Screening is hampered by the lack of true monospecific anti‐HLA‐B27 monoclonal antibodies. (currentprotocols.com)
  • HLA-DQ antibodies are likely also the most detrimental to graft survival. (californianewswire.com)
  • Human HLA specific antibodies crossreact with swine leucocyte antigens. (surrey.ac.uk)
  • Polyspecific HLA antibodies often crossreact with other HLA antigens through so called public and private determinants. (surrey.ac.uk)
  • Swine leucocyte antigens share approximately 75-87% homology with HLA and as such, it may be expected that some HLA antibodies would react with certain SLA antigens. (surrey.ac.uk)
  • Upon absorption of the HLA antibodies, the majority of anti-pig reactivity was abrogated. (surrey.ac.uk)
  • The eluates showed that HLA class I and II antibodies were eluted from pig splenocytes. (surrey.ac.uk)
  • We leveraged a collection of 14 ICI-resistant lung cancer samples to investigate whether alterations in genes encoding HLA Class I antigen processing and presentation machinery (APM) components or interferon signaling play a role in acquired resistance to PD-1 or PD-L1 antagonistic antibodies. (aacrjournals.org)
  • Research on human blood cells in the 1950s identified three genes associated with the HLA (HLA-A, HLA-B, HLA-C). In the 1970s, another gene was identified (HLA-D). With the advent of molecular technology beginning in the 1980s, more genes that code for proteins that function in the antigen complex have continued to be identified. (encyclopedia.com)
  • The major histocompatibility complex (MHC) in humans refers to a genetic region containing hundreds of genes, including the human leukocyte antigen (HLA) genes ( figure 1 ). (uptodate.com)
  • HLA genes express their gene products on the surface of white blood cells (hence the name 'human leukocyte antigen,' although HLA class I genes (see 'Class I region' below) are also expressed on all nucleated cells) and were originally recognized to contain the genes encoding 'tissue antigens' or 'tissue types. (uptodate.com)
  • Adapative immunity: Histocompatibility antigens and immune response genes. (uptodate.com)
  • In addition to in vitro binding measurements, MARIA is trained on peptide HLA ligand sequences identified by mass spectrometry, expression levels of antigen genes and protease cleavage signatures. (nature.com)
  • DNA test of HLA-related genes. (rochester.edu)
  • Genes in the MHC that may affect antigen processing. (springer.com)
  • Fling SP, Arp B, Pious D. HLA-DMA and -DMB genes are both required for MHC class II/peptide complex formation in antigen-presenting cells. (springer.com)
  • Defective processing and presentation of exogenous antigens in mutants with normal HLA class II genes. (springer.com)
  • n a collection of human genes on chromosome 6 that encode proteins that function in cells to transport antigens from within the cell to the cell surface. (thefreedictionary.com)
  • The Human Leukocyte Antigen (HLA) region, located on the short arm of chromosome 6 (6p21.3), is a highly polymorphic region containing about 200 genes. (bio-medicine.org)
  • The HLA region is the human equivalent of the Major Histocompatibility Complex (MHC), and as such contains a set of genes that serve as the backbone of antigen presentation. (bio-medicine.org)
  • The Class I proteins, classically involved in presenting endogenous antigens to CD8+ T-cells, are expressed by genes located in the HLA-A, -B and C loci. (bio-medicine.org)
  • Class I human histocompatibility (HLA) antigens encoded by a small cluster of structural genes at the C locus on chromosome 6. (curehunter.com)
  • The HLA-G gene has the same general structure as the classical MHC class I genes with five exons and three introns. (rupress.org)
  • In humans, MHC proteins are encoded by the Human Leukocyte Antigen (HLA), a group of more than 200 genes located closely together on the short arm of chromosome 6. (antibodies-online.com)
  • We investigated whether particular human leukocyte antigen (HLA) class I and class II genes contribute to the development of venom allergy. (aaem.pl)
  • Major histocompatibility complex (MHC) genes, also known as human leukocyte antigen (HLA) genes in humans, are the prominent susceptibility factor for many autoimmune diseases, including multiple sclerosis (MS), autoimmune diabetes (type 1 diabetes or T1D), and rheumatoid arthritis (RA). (sciencemag.org)
  • HLA-D is a so-called class II major histocompatibility molecule. (encyclopedia.com)
  • At the outer surface of the cell the molecule contains an antigen that has been acquired from the surrounding environment. (encyclopedia.com)
  • However, cells from a family member that expressed HLA-B8 could present an epitope peptide restricted by that molecule. (rupress.org)
  • The specific expression of HLA-G in placental trophoblast suggests an important role for this molecule in the immunological interaction between mother and child. (rupress.org)
  • Selective expression of the human class Ib HLA molecule HLA-G in immunologically protected sites and its function in the inhibition of NK and T-cell effector functions support an important role of this molecule in immunoregulation. (diabetesjournals.org)
  • We hypothesize that the expression of HLA-G as an immunomodulatory molecule may be relevant at sites of organ-specific autoimmunity, such as pancreatic islets. (diabetesjournals.org)
  • HLA-E (human leucocyte antigen-E) is a conserved class I major histocompatibility molecule which has only limited polymorphism. (portlandpress.com)
  • Understanding the unique structural and molecular properties that make the HLA-DQ molecule so pathogenic may eventually help us better predict which mismatches will induce harmful antibody formation, and which are more permissible. (californianewswire.com)
  • Using chronically infected HLA-B35-TG,we tried to identify the T cell epitopes that HLA molecule presents to the CD8 cytotoxic T cells. (nii.ac.jp)
  • The earliest HLA associations with rheumatic diseases, such as the association of the HLA-B*27 allele at the HLA-B gene with ankylosing spondylitis (AS) risk and the association of the HLA-DRB1*04 allele at the HLA-DRB1 gene with rheumatoid arthritis (RA), were discovered several decades ago. (uptodate.com)
  • In some HLA-associated drug hypersensitivity reactions, the presence of a risk allele is a necessary but incomplete factor for disease development. (nih.gov)
  • While the presence of HLA-B*58:01 allele substantially increases the risk of allopurinol hypersensitivity, it is not an absolute requirement, suggesting that other factors also play an important role. (nih.gov)
  • Members of this subtype contain alpha chains that are encoded by the HLA-B*27 allele family. (harvard.edu)
  • We assessed HLA DRB1 allele frequency using low (two-digit) typing resolution. (haematologica.org)
  • In patients with HLA DRB1*01 allele, there was a trend towards a shorter time from diagnosis to treatment (log-rank test, p =0.07, Figure 1A . (haematologica.org)
  • Furthermore, HLA -A*01 allele frequency had a trend to be higher in Group 1 than in Group 2 (14.3 percent vs 4.1 percent, p=0.055). (aaem.pl)
  • Through its new ecommerce website, www.hlaprotein.com, Pure Protein now offers academic and commercial researchers the ability to purchase individual HLA reagents to detect, profile, and monitor allele-specific immune responses, as well as HLA peptide epitope binding services to aide in improving the design of vaccination and therapeutic targeting strategies. (californianewswire.com)
  • In type I, but not type II psoriasis, the Caucasian HLA extended haplotype (EH) Cw6-B57-DRB1*0701-DQA1*0201-DQB1*0303 named according to the B allele EH-57.1 was highly significantly overrepresented (p cor= 0.00021). (diva-portal.org)
  • The serotype identifies the B*38 allele products of the HLA-B gene-locus. (wikipedia.org)
  • Linkage studies indicate a factor in the HLA-class I region is more greatly associated, with HLA-B38 so far the only linked allele Marsh, S. G. (wikipedia.org)
  • For A11, the alpha "A" chain are encoded by the HLA-A*11 allele group and the β-chain are encoded by B2M locus. (wikipedia.org)
  • Allele Query Form IMGT/HLA - European Bioinformatics Institute Gregoriadis S, Zervas J, Varletzidis E, Toubis M, Pantazopoulos P, Fessas P (December 1982). (wikipedia.org)
  • The human leukocyte antigen (HLA) is not a single antigen, but is rather a group of proteins that are located on the surface of white blood cells. (encyclopedia.com)
  • Human leukocyte antigens (HLAs) are proteins that help the body's immune system tell the difference between its own cells and foreign, harmful substances. (medlineplus.gov)
  • Here, we show for the first time that prostate cancer cells express HLA class II proteins that are recognized by CD4+ T cells. (nih.gov)
  • As membrane proteins on their way to degradation in lysosomes as part of their normal turn-over are also contained in the endosomal/lysosomal compartments, exogenous antigens must compete with those derived from endogenous components. (rcsb.org)
  • In contrast, the Class II proteins, which associate with and present exogenous antigens to CD4+ T-cells, are expressed by the HLA DR, -DQ and DP loci. (bio-medicine.org)
  • The proteins coded for by the HLA-A and HLA-B loci in man and the H-2K and H-2D loci in mice were identified as cell surface receptors for Semliki Forest virus. (pnas.org)
  • ii) Isolated detergent-soluble HLA-A and HLA-B antigens reconstituted in lipid vesicles inhibit the binding of viral proteins to human cells (as do the water-soluble antigens to a lesser extent). (pnas.org)
  • iii) Reconstituted HLA-A and HLA-B vesicles interact in solution with Semliki Forest virus (or with vesicles containing viral spike proteins), as demonstrated by coprecipitation with antisera. (pnas.org)
  • iv) Complexes between viral spoke proteins and HLA-A and HLA-B antigens or H-2K and H-2D antigens can be isolated from the cell surface by utilizing affinity chromatography or immunoprecipitation. (pnas.org)
  • High-throughput epitope discovery reveals frequent recognition of neo-antigens by CD4 + T cells in human melanoma. (nature.com)
  • Epitope analysis of HLA-DQ antigens: what does the antibody see? (sigmaaldrich.com)
  • None of the eight B cell lines that expressed HLA-B27 presented a known peptide epitope to CTL. (rupress.org)
  • Rapid antigen processing and presentation of a protective and immunodominant HLA-B*27-restricted hepatitis C virus-specific CD8+ T-cell epitope. (doaj.org)
  • To better define the immunological mechanisms underlying HLA-B*27-mediated protection in HCV infection, we analyzed the functional avidity, functional profile, antiviral efficacy and naïve precursor frequency of CD8+ T cells targeting the immunodominant HLA-B*27-restricted HCV-specific epitope as well as its antigen processing and presentation. (doaj.org)
  • The HLA-B*27-restricted CD8+ T-cell epitope was not superior to epitopes restricted by HLA-A*02 when considering the functional avidity, functional profile, antiviral efficacy or naïve precursor frequency. (doaj.org)
  • However, the peptide region containing the HLA-B*27-restricted epitope was degraded extremely fast by both the constitutive proteasome and the immunoproteasome. (doaj.org)
  • This efficient proteasomal processing that could be blocked by proteasome inhibitors was highly dependent on the hydrophobic regions flanking the epitope and led to rapid and abundant presentation of the epitope on the cell surface of antigen presenting cells. (doaj.org)
  • Our data suggest that rapid antigen processing may be a key immunological feature of this protective and immunodominant HLA-B*27-restricted HCV-specific epitope. (doaj.org)
  • Here, we report a high degree of epitope overlap and T cell promiscuity between susceptible HLA-DQ8 and HLA-DQ8 transdimer. (sciencemag.org)
  • Using a method of epitope deduction, HLA-A3-restricted peptide epitopes were screened from hTERT and tested for immunogenicity in a human in vitro T-cell system. (aacrjournals.org)
  • HLA-A^*3303-rectricted CTL recognized 10-mer epitope ending Arg while HLA-A^*3101 -rectricted CTL recognized 9-mer epitope ending the same Arg. (nii.ac.jp)
  • Journal Article] Clinical relevance of a newly identified HLA-A24-restricted minor histocompatibility antigen epitope derived from BCL2A1,ACC-1,in patients receiving HLA genotypically matched unrelated bone marrow transplant. (nii.ac.jp)
  • A gene in the human major histocompatibility complex class II region controlling the class I antigen presentation pathway. (springer.com)
  • The purpose of this study was to investigate the prospect of antigen specific immunotherapy against prostate cancer via the HLA class II pathway of immune recognition. (nih.gov)
  • These β-cells also expressed mRNA for Class II and Class II antigen presentation pathway components, but lacked the macrophage marker CD68. (diabetesjournals.org)
  • Human leukocyte antigen (HLA)-DQ has emerged as the alloantibody most frequently associated with the generation of de novo donor-specific antibody (DSA), antibody-mediated-rejection, and unfavorable transplantation outcome. (sigmaaldrich.com)
  • Here, we have described Pr20, a TCR mimic (TCRm) human IgG1 antibody that recognizes the cell-surface ALY peptide/HLA-A2 complex. (jci.org)
  • An afucosylated Fc form (Pr20M) directed antibody-dependent cellular cytotoxicity against PRAME+HLA-A2+ leukemia cells and was therapeutically effective against mouse xenograft models of human leukemia. (jci.org)
  • This unit describes screening for HLA‐B27 on peripheral blood lymphocytes using more than one HLA‐B27 monoclonal antibody to detect possible cross‐reactivity with non‐HLA‐B27 antigens. (currentprotocols.com)
  • Advances in desensitization therapy and immunosuppressants made kidney transplantation possible in a recipient who had incompatible blood type with donor or preformed antibody against donor human leukocyte antigen (HLA). (ovid.com)
  • 1) positive result in crossmatch test and/or (2) donor specific antibody with mean fluorescent intensity (MFI) over 5000 measured by Luminex single antigen assay. (ovid.com)
  • A recipient who had combined immunologic risk of blood type incompatibility and preformed antibody against donor HLA antigen presented comparable outcome to a patient who had one of these risk factors. (ovid.com)
  • The Tambur lab at the Comprehensive Transplant Center, Northwestern University, Chicago, is focusing on understanding the immunogenicity of HLA-DQ antigens and its role in antibody-mediated rejection in solid organ transplantation," said Dr. Tambur. (californianewswire.com)
  • The presence of HLA specific antibody in the eluates was investigated. (surrey.ac.uk)
  • Some HLA specific sera still reacted against some pigs after removal of natural antibody and no reactivity was identical to any other. (surrey.ac.uk)
  • In addition, the presence of a non-HLA antibody was also found. (surrey.ac.uk)
  • The HLA-DR antigen was demonstrated by the mouse monoclonal antibody OKIa by a peroxidase-antiperoxidase method performed on glass slides. (uni-regensburg.de)
  • The serotype is determined by the antibody recognition of α11 subset of HLA-A α-chains. (wikipedia.org)
  • Our collaboration with Pure Transplant Solutions allows us to develop unique approaches to study the unique involvement of HLA-DQ in transplant immunology. (californianewswire.com)
  • Because the HLA is a chemical tag that distinguishes "self" from "nonself," the antigen is important in the rejection of transplanted tissue and in the development of certain diseases (e.g., insulin-dependent diabetes). (encyclopedia.com)
  • Defects in the structure of the HLAs is the cause of some diseases where the body's immune system perceives a host antigen as foreign and begins to attack the body's own tissue. (encyclopedia.com)
  • HLA testing is also used to match donated tissue with a person's tissue who is getting an organ transplant. (medlineplus.gov)
  • Human leukocyte antigen (HLA) testing is also called HLA typing or tissue typing. (cancer.ca)
  • A pattern of antigens, called a tissue type, is inherited from your parents. (cancer.ca)
  • The HLA system is used to assess tissue compatibility. (thefreedictionary.com)
  • DO was discovered in association with another chaperone HLA-DM (DM) but unlike DM, its distribution is more tissue specific, and its function more subtle. (frontiersin.org)
  • A clear understanding of the differences between HLA polymorphisms has provided ample insight into why and how foreign tissue is rejected by the host, and as such been a critical enabler of the field of transplantation. (bio-medicine.org)
  • Today, a variety of techniques are applied for HLA tissue typing, providing an important tool to increase the success rate of human transplants. (bio-medicine.org)
  • Furthermore, we provide evidence that HLA-G expressed in this tissue may associate with a subset of insulin-containing granules and may be shuttled to the cell surface in response to secretory stimuli. (diabetesjournals.org)
  • Interestingly, these tissues represent immunologically protected sites (the fetal trophoblast), sites of immune privilege (the anterior chamber of the eye and the testis), or lymphocyte selection (the thymus), suggesting a role for HLA-G in tissue-specific immunoregulation. (diabetesjournals.org)
  • After solubilization of tumor tissue and specific immunoprecipitation of classic HLA-A, -B, and -C and nonclassic HLA-G antigens the tumor samples were analyzed by one-dimensional isoelectric focusing (1D-IEF) and Western blot analysis. (arvojournals.org)
  • HLA-C and -G antigens were not found in uveal melanoma tissue implying a susceptibility for NK lysis. (arvojournals.org)
  • Many pathways of immune escape are known, including signaling defects in T cells, contact-induced anergy of T cells, and downregulation of HLA expression in tumor tissue. (arvojournals.org)
  • The differences between placental (fetal) Mφ and adult peritoneal Mφ may reflect both tissue-specific differences and generally diminished class II antigen expression on fetal and neonatal mononuclear phagocytes. (caltech.edu)
  • These preliminary data support a role for tissue matching in cadaver transplantation and suggest that combined matching for HLA AB and DR antigens may be more useful than matching for either alone. (mdedge.com)
  • Cytotoxic T lymphocytes(CTL) specific for minor histocompatibility antigens(mHAgs) whose tissue expression is limited to hematopoietic cells are useful for immunotherapy of relapsed leukemia/lymphoma following allogeneic hematopoietic cell transplantation(HCT). (nii.ac.jp)
  • In the present study we have tested the possibility of the role of 14bp insertion / deletion polymorphism of HLA-G in cancer progression and susceptibility. (aacrjournals.org)
  • Classical class I and class II Human Leukocyte Antigen (HLA) are leading candidates for infectious disease susceptibility. (antibodies-online.com)
  • Many observations point to a major role for classical HLA loci in determining susceptibility to viral infections 1 . (antibodies-online.com)
  • Analysis of HLA DR2&DQ6 (DRB1*1501, DQA1*0102, DQB1*0602) haplotypes in Iranian patients with multiple sclerosis. (harvard.edu)
  • Major Histocompatibility Complex Class II DR Beta 1 also known as HLA-DRB1 ia a member of the HLA class II beta chain paralogs. (prospecbio.com)
  • HLA-DRB1 produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 207 amino acids (30-227a.a.) and having a molecular mass of 24.0kDa. (prospecbio.com)
  • HLA-DRB1 is expressed with a 9 amino acid His tag at C-Terminus and purified by proprietary chromatographic techniques. (prospecbio.com)
  • HLA-DRB1 protein solution (0.25mg/ml) contains Phosphate Buffered Saline (pH 7.4) and 30% glycerol. (prospecbio.com)
  • We investigated HLA DRB1 correlations with chronic lymphocytic leukemia (B-CLL) outcome in 90 patients. (haematologica.org)
  • HLA DRB1*01 and HLA DRB1*02-null were associated with shorter overall survival ( p =0.007, p =0.002). (haematologica.org)
  • Allelic frequencies of HLA DRB1 were systematically examined in 90 B-CLL patients seen in the Department of Hematology during 2003-2004 for control visits, and in 94 ethnically-matched, healthy controls. (haematologica.org)
  • The HLA DRB1 allelic frequencies and distributions were consistent with the Hardy-Weinberg equilibrium, and did not differ significantly between CLL patients and the control group. (haematologica.org)
  • We were therefore unable to detect previously reported associations of HLA DRB1*0401 and DRB1*0403 with CLL incidence, or DRB3 (DR52) and DRB4 (DR53) supertypical loci with age-at-onset of CLL. (haematologica.org)
  • In a multivariate Cox regression model, the HLA DRB1*01 remained an independent factor predicting for shorter OS (p=0.005, relative risk [RR]= 3.84). (haematologica.org)
  • Serum soluble HLA class I antigen levels in hemodialysis patients and following renal transplantation. (biomedsearch.com)
  • We measured the serum levels of soluble HLA class I antigen (sHLA-I) to evaluate the immune status of uremia and following renal transplantation. (biomedsearch.com)
  • Long-term maintenance of HLA-D restricted T cells specific for soluble antigens. (diva-portal.org)
  • It seems important to recognize the function and role of soluble histocompatibility antigens in the pathogenesis of a disease. (medscimonit.com)
  • Soluble HLA-I antigens from the serum of CAH children demonstrate incorrect concentration level values in comparison with sHLA-I antigens of healthy individuals. (medscimonit.com)
  • Furthermore, these antigens are not expressed in the placenta with the exception of HLA‐C. However, HLA‐G is expressed on especially invasive cytotrophoblasts and exists in both membrane and soluble forms. (deepdyve.com)
  • Coordinate defects in human histocompatibility leukocyte antigen class II expression and antigen presentation in bare lymphocyte syndrome. (springer.com)
  • Essentially the different HLA arrangement on cells allows the immune system to develop an inventory of "self" antigens in the body. (encyclopedia.com)
  • HLA-B27 is a blood test to look for a protein that is found on the surface of white blood cells. (medlineplus.gov)
  • It is a blood test that identifies antigens on the surface of cells and tissues. (cancer.ca)
  • HLA-B27 is a human leukocyte antigen that helps the body differentiate its own cells from foreign substances. (reference.com)
  • Human leukocyte antigens help regulate the immune system, and their main role is to let the body tell the difference between normal cells and substances that it needs to attack, states MedlinePlus. (reference.com)
  • The presentation of antigen to T cells requires that the antigens first be processed prior to presentation. (springer.com)
  • Assembly and intracellular transport of HLA-DM and correction of the class II antigen-processing defect in T2 cells. (springer.com)
  • These Antigens are polymorphic Glycoproteins comprising alpha and beta chains and are found on lymphoid and other Cells , often associated with certain Diseases . (online-medical-dictionary.org)
  • Fais S, Pallone F, Squarcia O, et al: HLA-DR antigens on colonic epithelial cells in inflammatory bowel disease: I. Relation to the state of activation of lamina propria lymphocytes and to the epithelial expression of other surface markers. (springer.com)
  • McDonald GB, Jewell DP: Class II antigen (HLA-DR) expression by intestinal epithelial cells in inflammatory diseases of colon. (springer.com)
  • Darr AS, Fuggle SV, Ting A, et al: Anomolous expression of HLA-DR antigens on human colorectal cancer cells. (springer.com)
  • Malfunctions during any step of antigen processing could lead to the development of self-reactive T cells or defective immune response to pathogens. (frontiersin.org)
  • Although much has been accomplished regarding how antigens are processed and presented to T cells, many questions still remain unanswered, preventing the design of therapeutics for direct intervention with antigen processing. (frontiersin.org)
  • Primary dendritic cells (DCs) also to express HLA-DO. (rcsb.org)
  • Regulatory CD4 + T cells are another specialized subset that plays a fundamental role in the maintenance of immune tolerance to self-antigens. (frontiersin.org)
  • Adoptive cell therapy (ACT) is an emerging approach that necessitates defining robust and efficient methods for the in vitro expansion of antigen-specific T cells then infused into patients. (frontiersin.org)
  • To address this challenge, artificial antigen presenting cells (AAPCs) have been developed. (frontiersin.org)
  • They constitute a reliable and easily usable platform to stimulate and amplify antigen-specific CD4 + T cells. (frontiersin.org)
  • Finally, we discuss the potential interest of these AAPCs, both as fundamental tools to decipher CD4 + T cell responses and as reagents to generate clinical grade antigen-specific CD4 + T cells for immunotherapy. (frontiersin.org)
  • Besides CD4 + effector cells, CD4 + regulatory T cells (Tregs), consisting of thymus-derived or induced cells, maintain peripheral tolerance to self-antigens by regulating other types of immune cells ( 3 ). (frontiersin.org)
  • Surface expression of this HLA determinant in endocrine cells is regulated in response to growth and inflammatory stimuli. (diabetesjournals.org)
  • Thus, HLA-G presentation by endocrine cells may be regulated in concert with their secretory activity. (diabetesjournals.org)
  • The latter feature characterizes the expression of HLA-G. Thus, high levels of HLA-G were described in the blastocyst and the fetal cytotrophoblast during the 1st trimester of pregnancy, the epithelial cells of the anterior chamber of the eye, the testis, and the fetal liver ( 3 ). (diabetesjournals.org)
  • In addition, we have reported a restricted expression of HLA-G within the thymus in a select subset of medullary and subcortical epithelial cells ( 4 ). (diabetesjournals.org)
  • In support of this hypothesis, it was demonstrated that presentation of HLA-G on target cells downregulates effector functions in NK cells, antigen-specific CD8 + T cells, and monocytes through engagement of inhibitory receptors expressed on effector cells ( 5 - 9 ). (diabetesjournals.org)
  • Hence, it was shown that transgenic expression of viral antigens within pancreatic islets does not elicit an immune response even in the face of circulating autoreactive T-cells ( 10 ). (diabetesjournals.org)
  • Here we present a nanoparticle delivery system that facilitates presentation of an immunogenic measles antigen specifically in cancer cells. (sri.com)
  • Treatment with this system facilitates activation of a secondary immune response against cancer cells, bypassing the need to identify tumor-associated antigens or educate the immune system through a primary immune response. (sri.com)
  • Cytotoxic T cells directed to tumor antigens not expressed on normal melanocytes dominate HLA-A2.1-restricted immune repertoire to melanoma. (jimmunol.org)
  • CTL clones from three of the four subsets did not lyse melanocytes, but recognized fresh HLA-A2.1+ melanomas and defined three classes of epitopes, including unique Ags, common melanoma Ags, and Ags shared with neoplastic cells of different histologic origin. (jimmunol.org)
  • By a limiting dilution assay, designed to evaluate the frequency of HLA-A2-restricted CTL precursors (CTLp) directed to melanoma but not to melanocytes, such precursors were found in the peripheral blood or tumor site of five of six HLA-A2.1+ melanoma patients, and their frequency was much higher than the frequency of CTLp recognizing both tumor cells and the melanocytes. (jimmunol.org)
  • These results suggest that in melanoma patients most of the HLA-A2.1-restricted immune repertoire to melanoma is directly to epitopes expressed in the neoplastic but not in the normal cells of the melanocyte lineage. (jimmunol.org)
  • Cancerous cells start expressing HLA-G which in turn provides a shield for various immune responses. (aacrjournals.org)
  • β-Cell surface expression of HLA Class II was detected on a portion of CD45 − insulin + β-cells from donors with type 1 diabetes by immunofluorescence and flow cytometry. (diabetesjournals.org)
  • Melanoma cells derived from the other 2 patients showed neither surface expression of the HLA-A2 antigen nor presence of the corresponding mRNA. (aacrjournals.org)
  • TIL derived from patients whose melanoma cell lines had normal expression of HLA-A2 had a CD8 phenotype and were capable of lysing autologous melanoma cells. (aacrjournals.org)
  • In an attempt to restore HLA-A2 antigen expression in one of the melanoma cell lines that were HLA-A2 negative, we transfected these cells with the HLA-A2 gene subcloned in the pSV2-neo vector. (aacrjournals.org)
  • However, we observed selective recognition of the HLA-A2 expressing clones by autologous cultured peripheral blood lymphocytes (which contained CD8 cells) as well as allogeneic CD8 + TIL with a HLA-A2 restricted pattern of recognition. (aacrjournals.org)
  • Overall, our data suggest that selective down-regulation of HLA-A2 antigen expression in melanoma cells may represent one of the mechanisms by which tumor cells escape immunological recognition. (aacrjournals.org)
  • Because the expression of classic and nonclassic HLA antigens is crucial for the recognition and elimination of tumor cells by cytotoxic T and/or NK cells, we analyzed the HLA-A, -B, -C, and -G expression in uveal melanoma specimens from 18 patients. (arvojournals.org)
  • A considerable portion of the uveal melanomas tested showed a loss of classic HLA class I antigens, which may enable them to escape from the immunosurveillance of cytotoxic T cells. (arvojournals.org)
  • In damaged cells, such as virally infected or tumour cells, down-regulation of HLA-A, HLA-B and HLA-C production or inhibition of TAP prevents stabilization of HLA-E by the leader peptide. (portlandpress.com)
  • Under these circumstances, HLA-E does not reach the cell surface and the cell is then vulnerable to lysis by natural killer cells. (portlandpress.com)
  • The effect of donor lymphocyte infusion is mediated by donor T cells recognizing minor histocompatibility antigens. (haematologica.org)
  • T cells recognizing hematopoietic restricted minor histocompatibility antigens may induce selective graft- versus -leukemia reactivity, whereas broadly-expressed antigens may be targeted in graft- versus -host disease. (haematologica.org)
  • Results Three antigens were demonstrated to be expressed on primary leukemic cells of various origins as well as subtypes of non-malignant hematopoietic cells, whereas one antigen was selectively recognized on malignant hematopoietic cells with antigen presenting cell phenotype. (haematologica.org)
  • All antigens may have contributed to a graft- versus -leukemia effect, and one minor histocompatibility antigen (LB-SWAP70-1Q) has specific therapeutic value based on its in vivo immunodominance and strong presentation on leukemic cells of various origins, but absence of expression on cytokine-treated fibroblasts. (haematologica.org)
  • We and others recently demonstrated that Whole Genome Association scanning (WGAs) is an efficient method for high throughput identification of MiHA, 8 - 12 illustrated by the discovery of 10 novel MiHA as targets for CD8 + T cells in 2 different patients with leukemia who responded to DLI after HLA-matched alloSCT. (haematologica.org)
  • A nine-amino acid peptide derived from hTERT binds strongly to HLA-A2 antigen and elicits CTL responses against a broad panel of hTERT + tumors (but not hTERT + hematopoietic progenitor cells). (aacrjournals.org)
  • In contrast, highly enriched HLA-A3 + CD34 + peripheral blood progenitor cells or activated T cells were not lysed. (aacrjournals.org)
  • To improve the immunogenicity of exosomes as cancer vaccine, we prepared exosomes from heat-stressed carcinoembryonic antigen (CEA)-positive tumor cells (CEA + /HS-Exo) and tested the efficacy of these exosomes in the induction of CEA-specific antitumor immunity. (aacrjournals.org)
  • Moreover, in vitro incubation of lymphocytes from HLA-A*0201 + healthy donors and HLA-A*0201 + CEA + cancer patients with CEA + /HS-Exo-pulsed autologous dendritic cells induces HLA-A*0201-restricted and CEA-specific CTL response. (aacrjournals.org)
  • Exosomes derived from tumor antigen peptide-pulsed dendritic cells elicit potent tumor-specific immune responses ( 12 ). (aacrjournals.org)
  • Exosomes derived from tumor cells are also a source of shared tumor rejection antigens for CTL cross-priming in animal model ( 13 ). (aacrjournals.org)
  • HSP70 prepared from tumor cells or virus-infected cells can elicit potent antigen-specific CD8 + CTL response and therapeutic effects. (aacrjournals.org)
  • Evidence has revealed that by priming antigen-presenting cells, especially dendritic cells, HSP70 exhibits potent adjuvant functions in stimulating the host immune response ( 19 - 23 ) and has a potent antitumor effect in animal model ( 21 - 23 ). (aacrjournals.org)
  • A Minority of T Cells Recognizing Tumor-Associated Antigens Presented in Self-HLA Can Provoke Anti-Tumor Reactivity. (onmedica.com)
  • We investigated whether T cells with sufficient avidity to recognize naturally overexpressed self-antigens in the context of self-HLA can be found in the T-cell repertoire of healthy donors. (onmedica.com)
  • Minor histocompatibility antigen (MiHA)-specific T cells were used as model, as the influence of thymic selection on the T-cell repertoire directed against MiHA can be studied in both self (MiHApos donors)and non-self (MiHAneg donors) backgrounds. (onmedica.com)
  • Of the 16 unique HA-1H-specific T-cell clones, 5 T-cell clones derived from HA-1Hneg/HLA-A*02:01pos donors and 1 T-cell clone derived from an HA-1Hpos/HLA-A*02:01pos donor showed reactivity against HA-1Hpos target cells. (onmedica.com)
  • These results illustrate that self-HLA-restricted T cells specific for self-antigens like MiHA in MiHApos donors and TAA are present in peripheral blood of healthy individuals, but clinical efficacy would require highly effective in-vivo priming by peptide vaccination in the presence of proper adjuvants or in-vitro expansion of the low numbers of self-antigen-specific T cells of sufficient avidity to recognize endogenously processed antigen. (onmedica.com)
  • Evidence to support the role of HLA-G5 in allograft acceptance through induction of immunosuppressive/ regulatory T cells. (semanticscholar.org)
  • HLA-E protects glioma cells from NKG2D-mediated immune responses in vitro: implications for immune escape in vivo. (semanticscholar.org)
  • We next attempted to identify a gene encoding HLA-A^*3303 restricted, male specific mHAg using a CTL clone that preferentially lysed hematopoietic cells. (nii.ac.jp)
  • The effect of recombinant human interferon gamma (IFN-gamma) on the induction of HLA class II (HLA-DR, -DP, -DQ) antigen expression on human corneal epithelial (HCE) cells was examined in different stages of culture. (arvojournals.org)
  • Class II antigens were not detected on HCE cells in either stage without IFN-gamma treatment. (arvojournals.org)
  • IFN-gamma induced three class II antigens on HCE cells in both stages in a dose- and time-dependent manner but at different levels for each antigen (DR greater than DP greater than DQ). (arvojournals.org)
  • These findings indicate that the induction of class II antigens on HCE cells may be regulated by IFN-gamma independently for each of the antigens and that DQ induction may depend upon the differentiation of HCE cells in culture. (arvojournals.org)
  • Mouse monoclonal to HLA-DR.HLA-DR a human class II antigen of the major histocompatibility complex(MHC),is a transmembrane glycoprotein composed of an alpha chain (36 kDa) and a beta subunit(27kDa) expressed primarily on antigen presenting cells:B cells, monocytes, macrophages and thymic epithelial cells. (cylch.org)
  • HLA-DR is also expressed on activated T cells. (cylch.org)
  • In previous studies, genetically engineered K562 have been used to generate artificial antigen presenting cells (AAPC). (ovid.com)
  • CoEX-A2s were capable of stimulating antigen-specific CD8+ T cells both directly and indirectly via CoEX-A2 cross-dressed cells. (ovid.com)
  • The results suggest that these novel exosomes may provide a crucial reagent for generating antigen-specific CD8+ T cells for adoptive cell therapies against viral infection and tumors. (ovid.com)
  • Those T cells recognize the invaders antigen with their own HLA-class lmolecules. (nii.ac.jp)
  • later, the viable cells (T cells) were examined for their cytotoxic activity against peptide pulsed target cells (HLA-B35 Transfectant) by Chromium 51 release assay. (nii.ac.jp)
  • 2. Search for the major T cell antigens recognized by human T cells from the patients in the endemic area. (nii.ac.jp)
  • The expression of HLA-DR (Ia-like) antigens on human macrophages was investigated by analyses of cells from bronchoalveolar lavage fluid obtained from 12 patients with pulmonary sarcoidosis, six patients with extrinsic allergic alveolitis, nine patients with cryptogenic fibrosing alveolitis, 11 normal non-smokers, and 12 normal smokers. (uni-regensburg.de)
  • For many years HLA polymorphisms were typed by serological respons. (bio-medicine.org)
  • The second HLA DNA typing technique is to use the PCR amplification reaction directly to detect HLA polymorphisms. (bio-medicine.org)
  • Therefore, 61 RSA couples (with three or more spontaneous abortions) and 47 fertile control couples were HLA‐G genotyped by direct DNA sequencing and analyzed for specific polymorphisms. (deepdyve.com)
  • In the future, an invading organism that possesses one or some of these "non-self" antigens will be swiftly recognized as an invader and will be dealt with. (encyclopedia.com)
  • Tumor-associated antigens (TAA) are monomorphic self-antigens that are proposed as targets for immunotherapeutic approaches to treat malignancies. (onmedica.com)
  • Antigens determined by leukocyte loci found on chromosome 6, the major histocompatibility loci in humans. (nih.gov)
  • However, the advent of recombinant DNA technology, which paved the way to identifying genetic differences among the HLA loci directly, has led many laboratories to abandon classic serological typing methods. (bio-medicine.org)
  • In general, HLA DNA can be typed either by hybridizing labeled, sequence specific oligonucleotide probes to HLA loci amplified by the polymerase chain reaction (PCR), or by using PCR to amplify th e HLA DNA directly through differential primer extension. (bio-medicine.org)
  • for example, it is possible to construct PCR primers pre labeled with biotin, and then amplify biotin labeled HLA loci. (bio-medicine.org)
  • For those laboratories with access to Sequencers, it may be the most sensitive, specific and cost effective option to sequence certain HLA loci directly. (bio-medicine.org)
  • HLA-I Antigen Presentation and Tapasin Influence Immune Responses Against Malignant Brain Tumors - Considerations for Successful Immunotherapy. (lu.se)
  • Loss of HLA class I expression in prostate cancer: implications for immunotherapy. (semanticscholar.org)
  • Human leukocyte antigen: the major histocompatibility complex of man. (medlineplus.gov)
  • A gene required for class II restricted antigen presentation maps to the major histocompatibility complex. (springer.com)
  • Melanoma cell killing was CD3 and major histocompatibility complex Class I restricted in both cases, but HLA-A2 restricted in only one case. (aacrjournals.org)
  • Major histocompatibility complex products Class I (HLA Class I) antigens are not expressed on the surface of normal human hepatocytes but become so in pathological conditions. (kuleuven.be)
  • The protein is called human leukocyte antigen B27 (HLA-B27). (medlineplus.gov)
  • A type of protein made by the immune system that disarms or destroys a specific foreign substance (antigen) when it appears in the body. (cancer.ca)
  • Human Leukocyte antigen -G is an immunosuppressive protein with multiple functions. (aacrjournals.org)
  • Protein microarray is a screening tool that reproducibly detects serums reactivities to non-HLA antigens. (asnjournals.org)
  • Chakhtoura, M. , Souccar, N. , Al-Akl, N. and Abdelnoor, A. (2011) Human leukocyte antigen associations and c-reactive protein levels in lebanese patients with aggressive periodontitis-HLA and CRP in aggressive periodontitis. (scirp.org)
  • Pure Transplant Solutions, LLC was founded in 1999 in order to leverage the leading research in HLA protein of parent company, Pure Protein, LLC, into solutions to address a growing list of needs in organ transplantation. (californianewswire.com)
  • Pure Protein, LLC is a biotechnology company funded and managed by Emergent Technologies, Inc. that is focused on the development and commercialization of proprietary technologies related to the human leukocyte antigen (HLA) system, formed and exclusively licensed from the University of Oklahoma. (californianewswire.com)
  • We pay special attention to the HLA-I dedicated multifunctional protein, tapasin, and in relation to the different tapasin-dependency of HLA-I allomorphs we also discuss allomorph specific traits in maturation, structure and linkage to malignant diseases and brain tumors in particular. (lu.se)
  • The complete structure and gene map of the HLA region have been published [ 1,2 ]. (uptodate.com)
  • Third, identification of mHAg gene(s) encoding HLA-A^*3303 and -A^*3101-restricted mHAgs was conducted. (nii.ac.jp)
  • Journal Article] A Novel HLA-A^*3303-restricted minor histocompatibility antigen encoded by an unconventional open reading frame of human TMSB4Y gene. (nii.ac.jp)
  • The serum of 100 patients with myasthenia gravis and 441 of their first-degree relatives was studied for the presence of autoantibodies against several antigens. (bmj.com)
  • Fig. 5: MARIA identifies lymphoma immunoglobulin HLA-DR presentation hotspots in patients with MCL. (nature.com)
  • Patients may get an HLA-B27 antigen test to find the cause of joint swelling and pain. (reference.com)
  • 1. Human leucocyte AB antigens were determined by means of a lymphocyte toxicity test in 84 patients with essential hypertension and in 1000 blood donors. (clinsci.org)
  • 2. The prevalence of HLA B8 was 16.4% in hypertensive patients and 8.9% in controls ( P = 0.07). (clinsci.org)
  • 3. The prevalence of HLA B12 was 34.5% in hypertensive patients and 26.9% in the control group (N.S.). In WHO stage III hypertension HLA B12 was found in six out of 10 patients. (clinsci.org)
  • 6. A positive family history of hypertension tended to be more common in those patients with essential hypertension associated with HLA B8. (clinsci.org)
  • Antigen HLA-A29 was present in 16 of 20 patients (80%) with birdshot retinochoroidopathy, but only in 31 of 418 controls (7.4%) (P less than .0001). (nih.gov)
  • Of the 20 patients, 13 were also tested for evidence of an in vitro mitotic immune response to purified retinal S-antigen. (nih.gov)
  • To determine whether HLA-A21 restricted melanoma Ags exist that are not expressed on normal melanocytes, a panel of 478 T cell clones from six HLA-A21+ patients was selected for HLA-A2 restricted lysis of autologous tumor and then tested for differential recognition of HLA-A2.1+ melanomas and normal melanocytes. (jimmunol.org)
  • BACKGROUND: Alveolar macrophages from patients with sarcoidosis express increased quantities of HLA-DR during activation. (bmj.com)
  • METHODS: The relation between silver staining patterns of nucleoli and HLA-DR antigen expression was examined in alveolar macrophages collected by bronchoalveolar lavage from 11 patients with pulmonary sarcoidosis and 11 control subjects. (bmj.com)
  • The number of silver stained dots in alveolar macrophages correlated significantly with the intensity and the density of HLA-DR antigen expression in the patients with sarcoidosis. (bmj.com)
  • HLA-B27 is found in 90% of patients with ankylosing spondylitis and 80% in Reiter's disease. (specialtylabs.com)
  • Three common gp100 epitopes have been identified that are recognized in the context of HLA-A2 by TIL from different patients: G9154 (KTWGQYWQV), G9209 (ITDQVPFSV), and G9280 (YLEPGPVTA). (jimmunol.org)
  • Aim: The aims of this study were to deter- mine if there is an HLA-AP association in Lebanese patients, and to determine CRP levels in patients and compare them to those in healthy controls. (scirp.org)
  • Cullinan, M.P., Sachs, J., Wolf, E. and Seymour, G.J. (1980) The distribution of HLA-A and -B antigens in patients and their families with periodontitis. (scirp.org)
  • Because of the recent demonstration that expression of HLA Class I antigens may be selectively lost in several human tumors, including melanoma, we derived pairs of tumor infiltrating lymphocytes (TIL) and melanoma cell lines from 4 human lymphocytic antigen (HLA)-A2 + patients with metastatic melanoma. (aacrjournals.org)
  • On the other hand, TIL derived from the 2 patients whose melanoma cell lines had lost expression of HLA-A2 had a predominant CD4 phenotype and virtually no cytotoxic activity. (aacrjournals.org)
  • In parallel, patients were typed for HLA-A, -B, and -C class I antigens by PCR with sequence-specific primers (PCR-SSP). (arvojournals.org)
  • In addition, HLA-A2 and -G expression was investigated by immunohistochemistry in paraffin-embedded tumor sections from these patients. (arvojournals.org)
  • I have 3 patients with HLA 17-2-52B that seem to have biotoxin illness. (survivingmold.com)
  • We have looked at the per cent of non-HLA susceptible patients with confirmed mold illness. (survivingmold.com)
  • We analyzed the graft survival according to presence of rejection episode, the result was inferior in patients who had rejection episode, but there was not statistical significance (p=0.102) We compared graft survival of presented patients with standard ABOi KT patients (n=88) and sensitized to donor HLA antigen patients (n=51) performed same period in our institute and there was no statistical significance (p=0.788). (ovid.com)
  • Background Patients with hematologic malignancies can be successfully treated with donor lymphocyte infusion after HLA-matched allogeneic hematopoietic stem cell transplantation. (haematologica.org)
  • The focus of the collaboration is to develop and test novel HLA reagents created by PTS that may be used for enhanced analysis and characterization of DQ antigens, an HLA Class II type that is increasingly gaining importance in causing rejection in transplant patients. (californianewswire.com)
  • The hTERT peptide K973 was used to generate specific CD8 + CTLs from HLA-A3 + cancer patients and healthy individuals. (aacrjournals.org)
  • Then, we evaluated their ability to induce a CEA-specific immune response in vivo in HLA-A2.1/K b transgenic mice and CEA-specific CTL response in vitro in HLA-A*0201 + healthy donors and HLA-A*0201 + CEA + cancer patients. (aacrjournals.org)
  • To further evaluate the nature of the HLA association with psoriasis, HLA haplotypes of 60 patients with type 1 (early onset, positive family history) and 30 patients with type II (late onset, no family history) psoriasis were investigated by polymerase chain reaction sequence-specific oligonucleotide hybridization (HLA class II) and serology (HLA class I). Ethnically matched blood donors (146) served as controls. (diva-portal.org)
  • Histocompatibility (HLA) antigen phenotypes have been studied in 100 patients with ulcerative colitis, 100 with Crohn's disease, and 283 normal controls. (bmj.com)
  • There was no significant difference in antigen frequency between patients and controls. (bmj.com)
  • However, the incidence of HLA-B27 was increased in the patients complicated by ankylosing spondylitis and/or sacroiliitis in both ulcerative colitis and Crohn's disease. (bmj.com)
  • In contrast, none of the 29 IBD patients with "enteropathic" peripheral arthropathy had B27 antigen. (bmj.com)
  • Furthermore, ankylosing spondylitis was found more frequently in ulcerative colitis bearing HLA-B27 compared with non-B27 patients (P less than 0-01). (bmj.com)
  • In addition, 12 of 14 ulcerative colitis patients and five out of six Crohn's patients with HLA-B27 had total colitis, compared with the frequency of total colitis in non-B27 patients (P less than 0-024 and less than 0-03 respectively). (bmj.com)
  • This is a retrospective analysis of the results of HLA AB and DR antigen matching in 56 transfused cadaver transplant patients. (mdedge.com)
  • NY-ESO-1 is a member of the cancer-testis family of tumor antigens that elicits strong humoral and cellular immune responses in patients with NY-ESO-1-expressing cancers. (uzh.ch)
  • The characterization of HLA class II-restricted epitopes will be useful for the assessment of spontaneous and vaccine-induced immune responses of cancer patients against defined tumor antigens. (uzh.ch)
  • First we examined the impact of BCL2A1/A24 disparity on clinical outcome using 320 HLA-A24-positive patients receiving HLA-identical HCT.donor pairs. (nii.ac.jp)
  • Publications] Kenji Hirayama: 'T-Lymphoproliferative response of the patients with Chagas'desease to epimastigote antigen of T. curuzi in GUATEMALA' Jpn. (nii.ac.jp)
  • Publications] Kenji Hirayama: 'T-Lympho-proliferative response of the patients with Chagas' disease to epimastigote antigen of T.cruzi in Guatemala' Jpn.J.Trop.Med.Hyg. (nii.ac.jp)
  • Publications] Kenji Hirayama: 'T-Lymphoproliferative response of the patients with Chagas' disease to epimastigote antigen of T.cruzi in Gatemala' Jpn.J.Parasit.44 Suppl. (nii.ac.jp)
  • A higher frequency of HLA-B38 was noted psoriatic arthritis patients with erythroderma. (wikipedia.org)
  • This recognition occurs because the HLA groups are "read" by an immune cell called the T cell . (encyclopedia.com)
  • Antigen presentation profiling reveals recognition of lymphoma immunoglobulin neoantigens. (nature.com)
  • It has been suggested that the maternal NK recognition of this downregulation is balanced by the expression of HLA-G, thus preventing damage to the placenta. (rupress.org)
  • However, little is known about HLA-DQ8 transdimer-restricted CD4 T cell recognition, an event crucial for triggering HLA-DQ8 transdimer-specific anti-islet immunity. (sciencemag.org)
  • Previous studies have suggested that, in human melanoma, expression of HLA-A2 antigen is important for tumor cell recognition by autologous T-lymphocytes. (aacrjournals.org)
  • The generation of HLA-DQ de novo DSA was interrogated in 40 transplant recipients who were immunologically naive before their failed transplantation. (sigmaaldrich.com)
  • This will pave the way to identifying acceptable mismatches and will allow risk stratification for generating de novo HLA-DSA after transplantation. (sigmaaldrich.com)
  • Rationale: Chemotherapy with fludarabine, cyclophosphamide and anti-thymocyte globulin may induce the engraftment cross the immunologic barrier in the setting of HLA-haploidentical allogeneic hematopoietic cell transplantation. (clinicaltrials.gov)
  • AUSTIN, Texas, Mar 17, 2021 (SEND2PRESS NEWSWIRE) - Pure Transplant Solutions, LLC (PTS), a collaboration driven biotechnology company focused on the development of human leukocyte antigen (HLA)-based diagnostics and therapeutics within the field of transplantation, is proud to announce that it has entered into a collaboration agreement with Northwestern University, a world leader in HLA DQ antigen research. (californianewswire.com)
  • In the last years, we have seen a re-emerging role of HLA-DQ in transplantation medicine with increasing evidence that DQ mismatches between transplant recipient and donor are most detrimental to graft survival," said Dr. Buchli. (californianewswire.com)
  • Conditions such as arthritis, ankylosing spondylitis and inflammation of the sacroiliac joint may cause an increase in HLA-B27 and human leukocyte antigens, states MedlinePlus. (reference.com)
  • for example, HLA-B27 is usually present in people who have ankylosing spondylitis. (thefreedictionary.com)
  • Ankylosing spondylitis is 10 times more common among individuals with HLA-B27 compared to individuals without this antigen. (specialtylabs.com)
  • A group of the D-related HLA Antigens found to differ from the DR Antigens in Genetic Locus and therefore inheritance. (online-medical-dictionary.org)
  • 5. In view of a previous report of HLA antigens in a Spanish diabetic population, this study does not support the suggestion of a genetic and possibly HLA-linked connection between essential hypertension and diabetes mellitus among the Spanish population. (clinsci.org)
  • Our findings suggested that birdshot retinochoroidopathy has a genetic predisposition and that retinal autoimmunity, resulting from the S-antigen or other retinal antigens, plays a role in the manifestation of this disease. (nih.gov)
  • Despite the contribution of HLA to the overall genetic risk has been estimated to range from 30% to 50%, it has never been studied in the French west-Indian population. (bioportfolio.com)
  • HLA-C at 6p21.33) in multi-ethnic populations with genetic heterogeneity and racial/ethnic differences among Caucasians, African-Americans, and Hispanics. (curehunter.com)
  • Although a tendency towards an association with a certain genetic type and with HLA-B51 is suspected, the incidence of several siblings with Behçet's disease in a single family is rare. (bmj.com)
  • Genetic aspect of venom allergy: association with HLA class I and class II antigens. (aaem.pl)
  • The detection of the HLA‐B27 antigen by immunomagnetic separation and enzyme‐linked immunosorbent assay: Comparison with a flow cytometric procedure. (currentprotocols.com)
  • In the first stage of the experiment we determined the appearance of CAH HLA-A, B, C lymphocyte antigens in comparison with 1152 healthy individuals. (medscimonit.com)
  • Nine human liver specimens, known from light microscopic investigation to display membranous positivity for HLA Class I antigens, were processed for immunoelectronmicroscopy using monoclonal anti-HLA Class I in an indirect immunoperoxidase procedure. (kuleuven.be)
  • Class Ia HLAs, namely HLA-A, -B, and -C, are heterodimers comprising a 45-kDa polymorphic heavy chain noncovalently associated with an invariant light chain, β2-microglobulin. (diabetesjournals.org)
  • The characterization of hTERT as a polyepitope, polyallelic tumor-associated antigen may provide an approach for circumventing therapy-induced resistance potentially mediated by antigenic- and allelic-loss tumor escape mutants. (aacrjournals.org)
  • Routine HLA‐B27 typing by flow cytometry: Differentiation of the products of HLA‐B*2702, B*2705 and B*2708. (currentprotocols.com)
  • CD4 + TIL showed no difference in the proliferative response to autologous parental and HLA-A2 transfected clones. (aacrjournals.org)
  • The results will show the degree to which HLA antigens match between you and the donor. (rochester.edu)
  • They have significantly lower immunogenicity than the HLA-A and -B determinants and are therefore of minor importance in donor/recipient crossmatching. (curehunter.com)
  • We examined these surface antigens and a monocyte marker antigen on fresh cord and adult blood monocytes, macrophages (Mφ) derived from monocytes in vitro, human placental (fetal) Mφ, from adult women. (caltech.edu)
  • Natali PG, Martino CD, Quaranta V, et al: Expression of lalike antigens in normal human nonlymphoid tissues. (springer.com)
  • Chiba M, Iizuka M, Masamune O: Ubiquitous expression of HLA-DR antigens on human small intestinal epithelium. (springer.com)
  • Iizuka M, Chiba M, Ohta H, et al: Expression of HLA-DR antigens on colonic epithelium in ulcerative colitis. (springer.com)
  • Selby WS, Janossy G, Mason DY, et al: Expression of HLA-DR antigens by colonic epithelium in inflammatory bowel disease. (springer.com)
  • Disease progression despite protective HLA expression in an HIV-infected transmission pair. (harvard.edu)
  • Correlation of morphological patterns of nucleoli in alveolar macrophages with HLA-DR antigen expression in sarcoidosis. (bmj.com)
  • Because silver staining has been described as a sensitive indicator of cellular activity a study was performed to examine whether it relates to HLA-DR antigen expression. (bmj.com)
  • The +14bp/-14bp and −14bp/-14bp genotypes have already been shown to be associated with higher expression of HLA-G and its mRNA stability and might be providing favorable micro-environment for cancer progression. (aacrjournals.org)
  • This especially concerns HLA antigen concentration levels (sHLA-1) in their different allogenic expression. (medscimonit.com)
  • We also observed some correlation between loss of HLA-A2 expression and level of c- myc transcription. (aacrjournals.org)
  • Preincubation of the HLA-A2 negative melanoma cell lines with α- or γ-interferon did not induce the re-expression of the HLA-A2 antigen. (aacrjournals.org)
  • Four transfected clones, with high levels of HLA-A2 antigen expression, were expanded and characterized. (aacrjournals.org)
  • In 9 (50%) of 18 specimens, a full HLA-A and -B antigen expression pattern was detected by 1D-IEF. (arvojournals.org)
  • Three to four parameters-forward scatter, side scatter, HLA‐B27 expression, and, in some cases CD3 or B7 expression-are acquired. (currentprotocols.com)
  • The purpose of this study was to specify the ultrastructural topography of HLA Class I antigens expression. (kuleuven.be)
  • Both the quantity and quality of peptide-HLA-I (pHLA-I) complexes are crucial for CTL responses, but the level of HLA-I expression per se is also directly involved in dictating NK-cell responses. (lu.se)
  • HLA class II antigen expression and IL-1 production by mononuclear phagocytes are important for antigen-stimulated T-cell activation. (caltech.edu)
  • These data indicate that class II antigen expression and IL-1 secretion by mononuclear phagocytes are only in part co-ordinately modulated. (caltech.edu)
  • Tumor-specific up-regulation of the nonclassical class I HLA-G antigen expression in renal carcinoma. (semanticscholar.org)
  • Lack of human leukocyte antigen-G expression in extravillous trophoblasts is associated with pre-eclampsia. (semanticscholar.org)
  • Regulation of HLA class II antigen expression on cultured corneal epithelium by interferon-gamma. (arvojournals.org)
  • The recommended ELISA Kit will likely detect the antigen in question with higher specificity in approved samples than the available alternatives. (antibodies-online.com)
  • One of the most commonly employed strategies in HLA DNA typing is to first amplify a given HLA locus, then hybridize it with a labeled, sequence specific oligonucleotide probe. (bio-medicine.org)
  • 1) HLA-B35 might not be ideal for the analysis of immune response against the cysteine protease antigen due to row affintiy. (nii.ac.jp)
  • Indeed much of the early knowledge of the antigen complex came from work on mice in the early decades of the twentieth century. (encyclopedia.com)
  • The human leukocyte antigen (HLA) complex is synonymous with the human MHC. (uptodate.com)
  • Positioning of autoimmune TCR-Ob.2F3 and TCR-Ob.3D1 on the MBP85-99/HLA-DR2 complex. (harvard.edu)
  • Soon after the entry of this complex into the endosomal/lysosomal system where antigen processing occurs, CD74 undergoes a sequential degradation by various proteases, including CTSS and CTSL , leaving a small fragment termed CLIP (class-II-associated invariant chain peptide). (rcsb.org)
  • The removal of CLIP is facilitated by HLA-DM via direct binding to the alpha-beta-CLIP complex so that CLIP is released. (rcsb.org)
  • 2 The Human Leukocyte Antigen complex (HLA) is one of the inherited factors that influences the development of B-CLL and clinical outcome of diffuse large B-cell lymphoma. (haematologica.org)
  • Mutagenesis at the α chain of HLA-DQ8 transdimer in complex with the disease-relevant GAD65 250-266 peptide and in silico analysis reveal the DQ α52 residue located within the N-terminal edge of the peptide-binding cleft for the enhanced T cell reactivity, altering avidity and biophysical affinity between TCR and HLA-peptide complexes. (sciencemag.org)
  • Despite preservation of putative residues for T cell receptor (TCR) contact, stronger disease-associated responses to cross-reactive, immunodominant islet epitopes are elicited by HLA-DQ8 transdimer. (sciencemag.org)
  • We have produced T cell clones reactive to epimastigote antigen. (nii.ac.jp)
  • Horie Y, Chiba M, Iizuka M, et al: Colonie lymphoid cell subsets and epithelial HLA-DR antigens in familial polyposis coli. (springer.com)
  • HLA antigens in familial Guillain-Barré syndrome. (bmj.com)
  • HLA antigen familial study in complete Behçet's syndrome affecting three sisters. (bmj.com)
  • No differences were found in the percentage of alveolar macrophages that expressed DR antigens between the five study groups. (uni-regensburg.de)
  • These observations suggest that the previously reported enhanced antigen presentation by alveolar macrophages in sarcoidosis is not linked with an increase in the percentage of DR+ macrophages in the lung. (uni-regensburg.de)
  • The HLA region lies on the short arm of chromosome six at position 6p21.3. (uptodate.com)