A promyelocytic cell line derived from a patient with ACUTE PROMYELOCYTIC LEUKEMIA. HL-60 cells lack specific markers for LYMPHOID CELLS but express surface receptors for FC FRAGMENTS and COMPLEMENT SYSTEM PROTEINS. They also exhibit phagocytic activity and responsiveness to chemotactic stimuli. (From Hay et al., American Type Culture Collection, 7th ed, pp127-8)
A malignant disease characterized by progressive enlargement of the lymph nodes, spleen, and general lymphoid tissue. In the classical variant, giant usually multinucleate Hodgkin's and REED-STERNBERG CELLS are present; in the nodular lymphocyte predominant variant, lymphocytic and histiocytic cells are seen.
An enzyme of the hydrolase class that catalyzes the reaction of triacylglycerol and water to yield diacylglycerol and a fatty acid anion. It is produced by glands on the tongue and by the pancreas and initiates the digestion of dietary fats. (From Dorland, 27th ed) EC 3.1.1.3.
An American National Standards Institute-accredited organization working on specifications to support development and advancement of clinical and administrative standards for healthcare.
Large cells, usually multinucleate, whose presence is a common histologic characteristic of classical HODGKIN DISEASE.
An acute myeloid leukemia in which abnormal PROMYELOCYTES predominate. It is frequently associated with DISSEMINATED INTRAVASCULAR COAGULATION.
A highly polar organic liquid, that is used widely as a chemical solvent. Because of its ability to penetrate biological membranes, it is used as a vehicle for topical application of pharmaceuticals. It is also used to protect tissue during CRYOPRESERVATION. Dimethyl sulfoxide shows a range of pharmacological activity including analgesia and anti-inflammation.
An antilipemic agent which lowers cholesterol, triglycerides, serum beta-lipoproteins and phospholipids. It acts by interfering with the enzymatic steps involved in the conversion of acetate to hydroxymethylglutaryl coenzyme A as well as inhibiting the activity of HYDROXYMETHYLGLUTARYL COA REDUCTASES which is the rate limiting enzyme in the biosynthesis of cholesterol.
An enzyme of the hydrolase class that catalyzes the reaction of triacylglycerol and water to yield diacylglycerol and a fatty acid anion. The enzyme hydrolyzes triacylglycerols in chylomicrons, very-low-density lipoproteins, low-density lipoproteins, and diacylglycerols. It occurs on capillary endothelial surfaces, especially in mammary, muscle, and adipose tissue. Genetic deficiency of the enzyme causes familial hyperlipoproteinemia Type I. (Dorland, 27th ed) EC 3.1.1.34.
Progressive restriction of the developmental potential and increasing specialization of function that leads to the formation of specialized cells, tissues, and organs.
Leukocytes with abundant granules in the cytoplasm. They are divided into three groups according to the staining properties of the granules: neutrophilic, eosinophilic, and basophilic. Mature granulocytes are the NEUTROPHILS; EOSINOPHILS; and BASOPHILS.
Cells grown in vitro from neoplastic tissue. If they can be established as a TUMOR CELL LINE, they can be propagated in cell culture indefinitely.
A rare, metallic element designated by the symbol, Ga, atomic number 31, and atomic weight 69.72.
A phorbol ester found in CROTON OIL with very effective tumor promoting activity. It stimulates the synthesis of both DNA and RNA.
Tetracyclic spiro-BENZAZEPINES isolated from the seeds of CEPHALOTAXUS. They are esters of the alkaloid cephalotaxine and may be effective as antineoplastic agents.
A large lobed glandular organ in the abdomen of vertebrates that is responsible for detoxification, metabolism, synthesis and storage of various substances.
A complex of related glycopeptide antibiotics from Streptomyces verticillus consisting of bleomycin A2 and B2. It inhibits DNA metabolism and is used as an antineoplastic, especially for solid tumors.
Form of leukemia characterized by an uncontrolled proliferation of the myeloid lineage and their precursors (MYELOID PROGENITOR CELLS) in the bone marrow and other sites.
Clonal expansion of myeloid blasts in bone marrow, blood, and other tissue. Myeloid leukemias develop from changes in cells that normally produce NEUTROPHILS; BASOPHILS; EOSINOPHILS; and MONOCYTES.
An antineoplastic agent used primarily in combination with mechlorethamine, vincristine, and prednisone (the MOPP protocol) in the treatment of Hodgkin's disease.
An important regulator of GENE EXPRESSION during growth and development, and in NEOPLASMS. Tretinoin, also known as retinoic acid and derived from maternal VITAMIN A, is essential for normal GROWTH; and EMBRYONIC DEVELOPMENT. An excess of tretinoin can be teratogenic. It is used in the treatment of PSORIASIS; ACNE VULGARIS; and several other SKIN DISEASES. It has also been approved for use in promyelocytic leukemia (LEUKEMIA, PROMYELOCYTIC, ACUTE).
Established cell cultures that have the potential to propagate indefinitely.
A general term for the complete or partial loss of the ability to hear from one or both ears.
A system containing any combination of computers, computer terminals, printers, audio or visual display devices, or telephones interconnected by telecommunications equipment or cables: used to transmit or receive information. (Random House Unabridged Dictionary, 2d ed)
The creation and maintenance of medical and vital records in multiple institutions in a manner that will facilitate the combined use of the records of identified individuals.
Antitumor alkaloid isolated from Vinca rosea. (Merck, 11th ed.)
Specific languages used to prepare computer programs.
Antineoplastic antibiotic obtained from Streptomyces peucetius. It is a hydroxy derivative of DAUNORUBICIN.
The procedures involved in combining separately developed modules, components, or subsystems so that they work together as a complete system. (From McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)
An antineoplastic agent. It has significant activity against melanomas. (from Martindale, The Extra Pharmacopoeia, 31st ed, p564)
Infection with human herpesvirus 4 (HERPESVIRUS 4, HUMAN); which may facilitate the development of various lymphoproliferative disorders. These include BURKITT LYMPHOMA (African type), INFECTIOUS MONONUCLEOSIS, and oral hairy leukoplakia (LEUKOPLAKIA, HAIRY).
Epithelial hyperplasia of the oral mucosa associated with Epstein-Barr virus (HERPESVIRUS 4, HUMAN) and found almost exclusively in persons with HIV infection. The lesion consists of a white patch that is often corrugated or hairy.
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
Diamond nanoparticles that exhibit unique biological, thermal, mechanical, and optoelectronic properties. They have important NANOMEDICINE applications including DRUG DELIVERY SYSTEMS; DIAGNOSTIC IMAGING; protein separation; and BIOSENSING TECHNIQUES.
Enzymes that catalyze the cleavage of a carbon-carbon bond of a 3-hydroxy acid. (Dorland, 28th ed) EC 4.1.3.
Information systems, usually computer-assisted, designed to store, manipulate, and retrieve information for planning, organizing, directing, and controlling administrative and clinical activities associated with the provision and utilization of clinical laboratory services.
A progressive, malignant disease of the blood-forming organs, characterized by distorted proliferation and development of leukocytes and their precursors in the blood and bone marrow. Leukemias were originally termed acute or chronic based on life expectancy but now are classified according to cellular maturity. Acute leukemias consist of predominately immature cells; chronic leukemias are composed of more mature cells. (From The Merck Manual, 2006)
Wearable sound-amplifying devices that are intended to compensate for impaired hearing. These generic devices include air-conduction hearing aids and bone-conduction hearing aids. (UMDNS, 1999)
One of the mechanisms by which CELL DEATH occurs (compare with NECROSIS and AUTOPHAGOCYTOSIS). Apoptosis is the mechanism responsible for the physiological deletion of cells and appears to be intrinsically programmed. It is characterized by distinctive morphologic changes in the nucleus and cytoplasm, chromatin cleavage at regularly spaced sites, and the endonucleolytic cleavage of genomic DNA; (DNA FRAGMENTATION); at internucleosomal sites. This mode of cell death serves as a balance to mitosis in regulating the size of animal tissues and in mediating pathologic processes associated with tumor growth.
The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.
Computer-based systems for input, storage, display, retrieval, and printing of information contained in a patient's medical record.
The physiologically active form of vitamin D. It is formed primarily in the kidney by enzymatic hydroxylation of 25-hydroxycholecalciferol (CALCIFEDIOL). Its production is stimulated by low blood calcium levels and parathyroid hormone. Calcitriol increases intestinal absorption of calcium and phosphorus, and in concert with parathyroid hormone increases bone resorption.
RNA sequences that serve as templates for protein synthesis. Bacterial mRNAs are generally primary transcripts in that they do not require post-transcriptional processing. Eukaryotic mRNA is synthesized in the nucleus and must be exported to the cytoplasm for translation. Most eukaryotic mRNAs have a sequence of polyadenylic acid at the 3' end, referred to as the poly(A) tail. The function of this tail is not known for certain, but it may play a role in the export of mature mRNA from the nucleus as well as in helping stabilize some mRNA molecules by retarding their degradation in the cytoplasm.
The rate dynamics in chemical or physical systems.
Abnormal growths of tissue that follow a previous neoplasm but are not metastases of the latter. The second neoplasm may have the same or different histological type and can occur in the same or different organs as the previous neoplasm but in all cases arises from an independent oncogenic event. The development of the second neoplasm may or may not be related to the treatment for the previous neoplasm since genetic risk or predisposing factors may actually be the cause.
A member of the tumor necrosis factor receptor superfamily that may play a role in the regulation of NF-KAPPA B and APOPTOSIS. They are found on activated T-LYMPHOCYTES; B-LYMPHOCYTES; NEUTROPHILS; EOSINOPHILS; MAST CELLS and NK CELLS. Overexpression of CD30 antigen in hematopoietic malignancies make the antigen clinically useful as a biological tumor marker. Signaling of the receptor occurs through its association with TNF RECEPTOR-ASSOCIATED FACTORS.
The type species of LYMPHOCRYPTOVIRUS, subfamily GAMMAHERPESVIRINAE, infecting B-cells in humans. It is thought to be the causative agent of INFECTIOUS MONONUCLEOSIS and is strongly associated with oral hairy leukoplakia (LEUKOPLAKIA, HAIRY;), BURKITT LYMPHOMA; and other malignancies.
Measurement of hearing based on the use of pure tones of various frequencies and intensities as auditory stimuli.
An enzyme found mostly in plant tissue. It hydrolyzes glycerophosphatidates with the formation of a phosphatidic acid and a nitrogenous base such as choline. This enzyme also catalyzes transphosphatidylation reactions. EC 3.1.4.4.
A biologic alkylating agent that exerts its cytotoxic effects by forming DNA ADDUCTS and DNA interstrand crosslinks, thereby inhibiting rapidly proliferating cells. The hydrochloride is an antineoplastic agent used to treat HODGKIN DISEASE and LYMPHOMA.
Large, phagocytic mononuclear leukocytes produced in the vertebrate BONE MARROW and released into the BLOOD; contain a large, oval or somewhat indented nucleus surrounded by voluminous cytoplasm and numerous organelles.
Any of a group of malignant tumors of lymphoid tissue that differ from HODGKIN DISEASE, being more heterogeneous with respect to malignant cell lineage, clinical course, prognosis, and therapy. The only common feature among these tumors is the absence of giant REED-STERNBERG CELLS, a characteristic of Hodgkin's disease.
Using certified ELECTRONIC HEALTH RECORDS technology to improve quality, safety, efficiency, and reduce HEALTHCARE DISPARITIES; engage patients and families in their health care; improve care coordination; improve population and public health; while maintaining privacy and security.
An antitumor alkaloid isolated from VINCA ROSEA. (Merck, 11th ed.)
Lipid-protein complexes involved in the transportation and metabolism of lipids in the body. They are spherical particles consisting of a hydrophobic core of TRIGLYCERIDES and CHOLESTEROL ESTERS surrounded by a layer of hydrophilic free CHOLESTEROL; PHOSPHOLIPIDS; and APOLIPOPROTEINS. Lipoproteins are classified by their varying buoyant density and sizes.
A formylated tripeptide originally isolated from bacterial filtrates that is positively chemotactic to polymorphonuclear leucocytes, and causes them to release lysosomal enzymes and become metabolically activated.
Degree to which individuals have the capacity to obtain, process, and understand basic health information and services needed to make appropriate health decisions.
Integrated, computer-assisted systems designed to store, manipulate, and retrieve information concerned with the administrative and clinical aspects of providing medical services within the hospital.
A semisynthetic derivative of PODOPHYLLOTOXIN that exhibits antitumor activity. Etoposide inhibits DNA synthesis by forming a complex with topoisomerase II and DNA. This complex induces breaks in double stranded DNA and prevents repair by topoisomerase II binding. Accumulated breaks in DNA prevent entry into the mitotic phase of cell division, and lead to cell death. Etoposide acts primarily in the G2 and S phases of the cell cycle.
A class of lipoproteins of small size (4-13 nm) and dense (greater than 1.063 g/ml) particles. HDL lipoproteins, synthesized in the liver without a lipid core, accumulate cholesterol esters from peripheral tissues and transport them to the liver for re-utilization or elimination from the body (the reverse cholesterol transport). Their major protein component is APOLIPOPROTEIN A-I. HDL also shuttle APOLIPOPROTEINS C and APOLIPOPROTEINS E to and from triglyceride-rich lipoproteins during their catabolism. HDL plasma level has been inversely correlated with the risk of cardiovascular diseases.
Part of an ear examination that measures the ability of sound to reach the brain.
Hearing loss resulting from damage to the COCHLEA and the sensorineural elements which lie internally beyond the oval and round windows. These elements include the AUDITORY NERVE and its connections in the BRAINSTEM.
A cell line derived from cultured tumor cells.
An ERYTHROLEUKEMIA cell line derived from a CHRONIC MYELOID LEUKEMIA patient in BLAST CRISIS.
A synthetic anti-inflammatory glucocorticoid derived from CORTISONE. It is biologically inert and converted to PREDNISOLONE in the liver.
Conversion of an inactive form of an enzyme to one possessing metabolic activity. It includes 1, activation by ions (activators); 2, activation by cofactors (coenzymes); and 3, conversion of an enzyme precursor (proenzyme or zymogen) to an active enzyme.
Elements of limited time intervals, contributing to particular results or situations.
Compounds with two triple bonds. Some of them are CYTOTOXINS.
Substances that inhibit or prevent the proliferation of NEOPLASMS.
A 9-kDa protein component of VERY-LOW-DENSITY LIPOPROTEINS. It contains a cofactor for LIPOPROTEIN LIPASE and activates several triacylglycerol lipases. The association of Apo C-II with plasma CHYLOMICRONS; VLDL, and HIGH-DENSITY LIPOPROTEINS is reversible and changes rapidly as a function of triglyceride metabolism. Clinically, Apo C-II deficiency is similar to lipoprotein lipase deficiency (HYPERLIPOPROTEINEMIA TYPE I) and is therefore called hyperlipoproteinemia type IB.
The metabolic process of breaking down LIPIDS to release FREE FATTY ACIDS, the major oxidative fuel for the body. Lipolysis may involve dietary lipids in the DIGESTIVE TRACT, circulating lipids in the BLOOD, and stored lipids in the ADIPOSE TISSUE or the LIVER. A number of enzymes are involved in such lipid hydrolysis, such as LIPASE and LIPOPROTEIN LIPASE from various tissues.
SESQUITERPENES cyclized to one 10-carbon ring.
The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.
Proto-oncogene proteins fes are protein-tyrosine kinases with a central SH2 DOMAIN. It has been implicated in SIGNAL TRANSDUCTION PATHWAYS for CELL DIFFERENTIATION of a variety of cell types including MYELOID PROGENITOR CELLS. Fes proto-oncogene proteins also bind TUBULIN and promote MICROTUBULE assembly.
(Z)-9-Octadecenoic acid 1,2,3-propanetriyl ester.
That portion of the electromagnetic spectrum in the visible, ultraviolet, and infrared range.
Organic nitrogenous bases. Many alkaloids of medical importance occur in the animal and vegetable kingdoms, and some have been synthesized. (Grant & Hackh's Chemical Dictionary, 5th ed)
Granular leukocytes having a nucleus with three to five lobes connected by slender threads of chromatin, and cytoplasm containing fine inconspicuous granules and stainable by neutral dyes.
The ability or act of sensing and transducing ACOUSTIC STIMULATION to the CENTRAL NERVOUS SYSTEM. It is also called audition.
Resistance or diminished response of a neoplasm to an antineoplastic agent in humans, animals, or cell or tissue cultures.
Used as a support for ion-exchange chromatography.
Guanosine 5'-(trihydrogen diphosphate), monoanhydride with phosphorothioic acid. A stable GTP analog which enjoys a variety of physiological actions such as stimulation of guanine nucleotide-binding proteins, phosphoinositide hydrolysis, cyclic AMP accumulation, and activation of specific proto-oncogenes.
A ubiquitous family of proteins that transport PHOSPHOLIPIDS such as PHOSPHATIDYLINOSITOL and PHOSPHATIDYLCHOLINE between membranes. They play an important role in phospholipid metabolism during vesicular transport and SIGNAL TRANSDUCTION.
Conditions with excess LIPIDS in the blood.
Hearing loss in frequencies above 1000 hertz.
The use of two or more chemicals simultaneously or sequentially in the drug therapy of neoplasms. The drugs need not be in the same dosage form.
Compounds that inhibit the activity of DNA TOPOISOMERASES.
The audibility limit of discriminating sound intensity and pitch.
Agents obtained from higher plants that have demonstrable cytostatic or antineoplastic activity.
An serine-threonine protein kinase that requires the presence of physiological concentrations of CALCIUM and membrane PHOSPHOLIPIDS. The additional presence of DIACYLGLYCEROLS markedly increases its sensitivity to both calcium and phospholipids. The sensitivity of the enzyme can also be increased by PHORBOL ESTERS and it is believed that protein kinase C is the receptor protein of tumor-promoting phorbol esters.
The fission of a CELL. It includes CYTOKINESIS, when the CYTOPLASM of a cell is divided, and CELL NUCLEUS DIVISION.
A management function in which standards and guidelines are developed for the development, maintenance, and handling of forms and records.
Derivatives of phosphatidic acid in which the hydrophobic regions are composed of two fatty acids and a polar alcohol is joined to the C-3 position of glycerol through a phosphodiester bond. They are named according to their polar head groups, such as phosphatidylcholine and phosphatidylethanolamine.
The testing of the acuity of the sense of hearing to determine the thresholds of the lowest intensity levels at which an individual can hear a set of tones. The frequencies between 125 and 8000 Hz are used to test air conduction thresholds and the frequencies between 250 and 4000 Hz are used to test bone conduction thresholds.
The phenotypic manifestation of a gene or genes by the processes of GENETIC TRANSCRIPTION and GENETIC TRANSLATION.
Technique using an instrument system for making, processing, and displaying one or more measurements on individual cells obtained from a cell suspension. Cells are usually stained with one or more fluorescent dyes specific to cell components of interest, e.g., DNA, and fluorescence of each cell is measured as it rapidly transverses the excitation beam (laser or mercury arc lamp). Fluorescence provides a quantitative measure of various biochemical and biophysical properties of the cell, as well as a basis for cell sorting. Other measurable optical parameters include light absorption and light scattering, the latter being applicable to the measurement of cell size, shape, density, granularity, and stain uptake.
A condition of elevated levels of TRIGLYCERIDES in the blood.
A generic term for fats and lipoids, the alcohol-ether-soluble constituents of protoplasm, which are insoluble in water. They comprise the fats, fatty oils, essential oils, waxes, phospholipids, glycolipids, sulfolipids, aminolipids, chromolipids (lipochromes), and fatty acids. (Grant & Hackh's Chemical Dictionary, 5th ed)
Derivatives of acetamide that are used as solvents, as mild irritants, and in organic synthesis.
3 beta,14-Dihydroxy-5 beta-card-20(22)enolide. A cardenolide which is the aglycon of digitoxin. Synonyms: Cerberigenin; Echujetin; Evonogenin; Thevetigenin.
Benzoic acid esters or salts substituted with one or more iodine atoms.
Chemical substances, produced by microorganisms, inhibiting or preventing the proliferation of neoplasms.
DNA sequences which are recognized (directly or indirectly) and bound by a DNA-dependent RNA polymerase during the initiation of transcription. Highly conserved sequences within the promoter include the Pribnow box in bacteria and the TATA BOX in eukaryotes.
A nonionic polyoxyethylene-polyoxypropylene block co-polymer with the general formula HO(C2H4O)a(-C3H6O)b(C2H4O)aH. It is available in different grades which vary from liquids to solids. It is used as an emulsifying agent, solubilizing agent, surfactant, and wetting agent for antibiotics. Poloxamer is also used in ointment and suppository bases and as a tablet binder or coater. (Martindale The Extra Pharmacopoeia, 31st ed)
Proteins whose abnormal expression (gain or loss) are associated with the development, growth, or progression of NEOPLASMS. Some neoplasm proteins are tumor antigens (ANTIGENS, NEOPLASM), i.e. they induce an immune reaction to their tumor. Many neoplasm proteins have been characterized and are used as tumor markers (BIOMARKERS, TUMOR) when they are detectable in cells and body fluids as monitors for the presence or growth of tumors. Abnormal expression of ONCOGENE PROTEINS is involved in neoplastic transformation, whereas the loss of expression of TUMOR SUPPRESSOR PROTEINS is involved with the loss of growth control and progression of the neoplasm.
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
Simultaneous resistance to several structurally and functionally distinct drugs.
Hearing loss due to interference with the mechanical reception or amplification of sound to the COCHLEA. The interference is in the outer or middle ear involving the EAR CANAL; TYMPANIC MEMBRANE; or EAR OSSICLES.
A general term for various neoplastic diseases of the lymphoid tissue.
The biosynthesis of RNA carried out on a template of DNA. The biosynthesis of DNA from an RNA template is called REVERSE TRANSCRIPTION.
Systems composed of a computer or computers, peripheral equipment, such as disks, printers, and terminals, and telecommunications capabilities.
External or interstitial irradiation to treat lymphomas (e.g., Hodgkin's and non-Hodgkin's lymphomas) and lymph node metastases and also some autoimmune diseases, such as rheumatoid arthritis.
The relationship between the dose of an administered drug and the response of the organism to the drug.
MONOMERIC GTP-BINDING PROTEINS that were initially recognized as allosteric activators of the MONO(ADP-RIBOSE) TRANSFERASE of the CHOLERA TOXIN catalytic subunit. They are involved in vesicle trafficking and activation of PHOSPHOLIPASE D. This enzyme was formerly listed as EC 3.6.1.47
Regulatory proteins that act as molecular switches. They control a wide range of biological processes including: receptor signaling, intracellular signal transduction pathways, and protein synthesis. Their activity is regulated by factors that control their ability to bind to and hydrolyze GTP to GDP. EC 3.6.1.-.
A plant genus of the family Apiaceae.
A group of DITERPENES cyclized into 3-ring PHENANTHRENES.
Any of the processes by which nuclear, cytoplasmic, or intercellular factors influence the differential control of gene action in leukemia.
Compounds or agents that combine with an enzyme in such a manner as to prevent the normal substrate-enzyme combination and the catalytic reaction.
The span of viability of a cell characterized by the capacity to perform certain functions such as metabolism, growth, reproduction, some form of responsiveness, and adaptability.
The field of information science concerned with the analysis and dissemination of medical data through the application of computers to various aspects of health care and medicine.
Carbon-containing thiophosphoric acid derivatives. Included under this heading are compounds that have carbon bound to either SULFUR atom, or the OXYGEN atom of the SPO3 core structure.
A trisaccharide antigen expressed on glycolipids and many cell-surface glycoproteins. In the blood the antigen is found on the surface of NEUTROPHILS; EOSINOPHILS; and MONOCYTES. In addition, CD15 antigen is a stage-specific embryonic antigen.
An antiprotozoal agent produced by Streptomyces cinnamonensis. It exerts its effect during the development of first-generation trophozoites into first-generation schizonts within the intestinal epithelial cells. It does not interfere with hosts' development of acquired immunity to the majority of coccidial species. Monensin is a sodium and proton selective ionophore and is widely used as such in biochemical studies.
A negative regulatory effect on physiological processes at the molecular, cellular, or systemic level. At the molecular level, the major regulatory sites include membrane receptors, genes (GENE EXPRESSION REGULATION), mRNAs (RNA, MESSENGER), and proteins.
Membrane glycoproteins found in high concentrations on iron-utilizing cells. They specifically bind iron-bearing transferrin, are endocytosed with its ligand and then returned to the cell surface where transferrin without its iron is released.
Gradual bilateral hearing loss associated with aging that is due to progressive degeneration of cochlear structures and central auditory pathways. Hearing loss usually begins with the high frequencies then progresses to sounds of middle and low frequencies.
The introduction of a phosphoryl group into a compound through the formation of an ester bond between the compound and a phosphorus moiety.
ADP-RIBOSYLATION FACTOR 1 is involved in regulating intracellular transport by modulating the interaction of coat proteins with organelle membranes in the early secretory pathway. It is a component of COAT PROTEIN COMPLEX I. This enzyme was formerly listed as EC 3.6.1.47.
A human cell line established from a diffuse histiocytic lymphoma (HISTIOCYTIC LYMPHOMA, DIFFUSE) and displaying many monocytic characteristics. It serves as an in vitro model for MONOCYTE and MACROPHAGE differentiation.
Management of the organization of HEALTH FACILITIES.
Evaluation undertaken to assess the results or consequences of management and procedures used in combating disease in order to determine the efficacy, effectiveness, safety, and practicability of these interventions in individual cases or series.
A deoxyribonucleotide polymer that is the primary genetic material of all cells. Eukaryotic and prokaryotic organisms normally contain DNA in a double-stranded state, yet several important biological processes transiently involve single-stranded regions. DNA, which consists of a polysugar-phosphate backbone possessing projections of purines (adenine and guanine) and pyrimidines (thymine and cytosine), forms a double helix that is held together by hydrogen bonds between these purines and pyrimidines (adenine to thymine and guanine to cytosine).
Diminished or failed response of an organism, disease or tissue to the intended effectiveness of a chemical or drug. It should be differentiated from DRUG TOLERANCE which is the progressive diminution of the susceptibility of a human or animal to the effects of a drug, as a result of continued administration.
Intracellular fluid from the cytoplasm after removal of ORGANELLES and other insoluble cytoplasmic components.
Antibodies produced by a single clone of cells.
The intracellular transfer of information (biological activation/inhibition) through a signal pathway. In each signal transduction system, an activation/inhibition signal from a biologically active molecule (hormone, neurotransmitter) is mediated via the coupling of a receptor/enzyme to a second messenger system or to an ion channel. Signal transduction plays an important role in activating cellular functions, cell differentiation, and cell proliferation. Examples of signal transduction systems are the GAMMA-AMINOBUTYRIC ACID-postsynaptic receptor-calcium ion channel system, the receptor-mediated T-cell activation pathway, and the receptor-mediated activation of phospholipases. Those coupled to membrane depolarization or intracellular release of calcium include the receptor-mediated activation of cytotoxic functions in granulocytes and the synaptic potentiation of protein kinase activation. Some signal transduction pathways may be part of larger signal transduction pathways; for example, protein kinase activation is part of the platelet activation signal pathway.
A specified list of terms with a fixed and unalterable meaning, and from which a selection is made when CATALOGING; ABSTRACTING AND INDEXING; or searching BOOKS; JOURNALS AS TOPIC; and other documents. The control is intended to avoid the scattering of related subjects under different headings (SUBJECT HEADINGS). The list may be altered or extended only by the publisher or issuing agency. (From Harrod's Librarians' Glossary, 7th ed, p163)
The branch of biology dealing with the effect of light on organisms.
An imidazole derivative that is commonly used as a topical antifungal agent.
A highly acidic mucopolysaccharide formed of equal parts of sulfated D-glucosamine and D-glucuronic acid with sulfaminic bridges. The molecular weight ranges from six to twenty thousand. Heparin occurs in and is obtained from liver, lung, mast cells, etc., of vertebrates. Its function is unknown, but it is used to prevent blood clotting in vivo and vitro, in the form of many different salts.
Splitting the DNA into shorter pieces by endonucleolytic DNA CLEAVAGE at multiple sites. It includes the internucleosomal DNA fragmentation, which along with chromatin condensation, are considered to be the hallmarks of APOPTOSIS.
Neutral or negatively charged ligands bonded to metal cations or neutral atoms. The number of ligand atoms to which the metal center is directly bonded is the metal cation's coordination number, and this number is always greater than the regular valence or oxidation number of the metal. A coordination complex can be negative, neutral, or positively charged.
Information systems, usually computer-assisted, designed to store, manipulate, and retrieve information for planning, organizing, directing, and controlling administrative activities associated with the provision and utilization of radiology services and facilities.
Media that facilitate transportability of pertinent information concerning patient's illness across varied providers and geographic locations. Some versions include direct linkages to online consumer health information that is relevant to the health conditions and treatments related to a specific patient.
A 241-kDa protein synthesized only in the INTESTINES. It serves as a structural protein of CHYLOMICRONS. Its exclusive association with chylomicron particles provides an indicator of intestinally derived lipoproteins in circulation. Apo B-48 is a shortened form of apo B-100 and lacks the LDL-receptor region.
The most abundant protein component of HIGH DENSITY LIPOPROTEINS or HDL. This protein serves as an acceptor for CHOLESTEROL released from cells thus promoting efflux of cholesterol to HDL then to the LIVER for excretion from the body (reverse cholesterol transport). It also acts as a cofactor for LECITHIN CHOLESTEROL ACYLTRANSFERASE that forms CHOLESTEROL ESTERS on the HDL particles. Mutations of this gene APOA1 cause HDL deficiency, such as in FAMILIAL ALPHA LIPOPROTEIN DEFICIENCY DISEASE and in some patients with TANGIER DISEASE.
An imaging technique using compounds labelled with short-lived positron-emitting radionuclides (such as carbon-11, nitrogen-13, oxygen-15 and fluorine-18) to measure cell metabolism. It has been useful in study of soft tissues such as CANCER; CARDIOVASCULAR SYSTEM; and brain. SINGLE-PHOTON EMISSION-COMPUTED TOMOGRAPHY is closely related to positron emission tomography, but uses isotopes with longer half-lives and resolution is lower.
Phospholipids which have an alcohol moiety in ethereal linkage with a saturated or unsaturated aliphatic alcohol. They are usually derivatives of phosphoglycerols or phosphatidates. The other two alcohol groups of the glycerol backbone are usually in ester linkage. These compounds are widely distributed in animal tissues.
The terms, expressions, designations, or symbols used in a particular science, discipline, or specialized subject area.
The movement of materials (including biochemical substances and drugs) through a biological system at the cellular level. The transport can be across cell membranes and epithelial layers. It also can occur within intracellular compartments and extracellular compartments.
Studies used to test etiologic hypotheses in which inferences about an exposure to putative causal factors are derived from data relating to characteristics of persons under study or to events or experiences in their past. The essential feature is that some of the persons under study have the disease or outcome of interest and their characteristics are compared with those of unaffected persons.
The uptake of naked or purified DNA by CELLS, usually meaning the process as it occurs in eukaryotic cells. It is analogous to bacterial transformation (TRANSFORMATION, BACTERIAL) and both are routinely employed in GENE TRANSFER TECHNIQUES.
Electrophoresis in which a polyacrylamide gel is used as the diffusion medium.
The relationships between symbols and their meanings.
A bacterial genus of the order ACTINOMYCETALES.
A class of lipoproteins that carry dietary CHOLESTEROL and TRIGLYCERIDES from the SMALL INTESTINE to the tissues. Their density (0.93-1.006 g/ml) is the same as that of VERY-LOW-DENSITY LIPOPROTEINS.
In vitro method for producing large amounts of specific DNA or RNA fragments of defined length and sequence from small amounts of short oligonucleotide flanking sequences (primers). The essential steps include thermal denaturation of the double-stranded target molecules, annealing of the primers to their complementary sequences, and extension of the annealed primers by enzymatic synthesis with DNA polymerase. The reaction is efficient, specific, and extremely sensitive. Uses for the reaction include disease diagnosis, detection of difficult-to-isolate pathogens, mutation analysis, genetic testing, DNA sequencing, and analyzing evolutionary relationships.
Cholesterol which is contained in or bound to high-density lipoproteins (HDL), including CHOLESTEROL ESTERS and free cholesterol.
The complex series of phenomena, occurring between the end of one CELL DIVISION and the end of the next, by which cellular material is duplicated and then divided between two daughter cells. The cell cycle includes INTERPHASE, which includes G0 PHASE; G1 PHASE; S PHASE; and G2 PHASE, and CELL DIVISION PHASE.
Proteins prepared by recombinant DNA technology.
A type of familial lipid metabolism disorder characterized by a variable pattern of elevated plasma CHOLESTEROL and/or TRIGLYCERIDES. Multiple genes on different chromosomes may be involved, such as the major late transcription factor (UPSTREAM STIMULATORY FACTORS) on CHROMOSOME 1.
A positive regulatory effect on physiological processes at the molecular, cellular, or systemic level. At the molecular level, the major regulatory sites include membrane receptors, genes (GENE EXPRESSION REGULATION), mRNAs (RNA, MESSENGER), and proteins.
An aspect of personal behavior or lifestyle, environmental exposure, or inborn or inherited characteristic, which, on the basis of epidemiologic evidence, is known to be associated with a health-related condition considered important to prevent.
The lipid- and protein-containing, selectively permeable membrane that surrounds the cytoplasm in prokaryotic and eukaryotic cells.
A therapeutic approach, involving chemotherapy, radiation therapy, or surgery, after initial regimens have failed to lead to improvement in a patient's condition. Salvage therapy is most often used for neoplastic diseases.
The ratio of the density of a material to the density of some standard material, such as water or air, at a specified temperature.
Chemicals with two conjoined aromatic rings incorporating two nitrogen atoms and one of the carbons oxidized with a keto oxygen.
Any detectable and heritable change in the genetic material that causes a change in the GENOTYPE and which is transmitted to daughter cells and to succeeding generations.
Structurally related forms of an enzyme. Each isoenzyme has the same mechanism and classification, but differs in its chemical, physical, or immunological characteristics.
Family of retrovirus-associated DNA sequences (myc) originally isolated from an avian myelocytomatosis virus. The proto-oncogene myc (c-myc) codes for a nuclear protein which is involved in nucleic acid metabolism and in mediating the cellular response to growth factors. Truncation of the first exon, which appears to regulate c-myc expression, is crucial for tumorigenicity. The human c-myc gene is located at 8q24 on the long arm of chromosome 8.
Leukemia induced experimentally in animals by exposure to leukemogenic agents, such as VIRUSES; RADIATION; or by TRANSPLANTATION of leukemic tissues.
Surgical insertion of an electronic hearing device (COCHLEAR IMPLANTS) with electrodes to the COCHLEAR NERVE in the inner ear to create sound sensation in patients with residual nerve fibers.
A basic element found in nearly all organized tissues. It is a member of the alkaline earth family of metals with the atomic symbol Ca, atomic number 20, and atomic weight 40. Calcium is the most abundant mineral in the body and combines with phosphorus to form calcium phosphate in the bones and teeth. It is essential for the normal functioning of nerves and muscles and plays a role in blood coagulation (as factor IV) and in many enzymatic processes.
Persons who have experienced a prolonged survival after serious disease or who continue to live with a usually life-threatening condition as well as family members, significant others, or individuals surviving traumatic life events.
The treatment of a disease or condition by several different means simultaneously or sequentially. Chemoimmunotherapy, RADIOIMMUNOTHERAPY, chemoradiotherapy, cryochemotherapy, and SALVAGE THERAPY are seen most frequently, but their combinations with each other and surgery are also used.
Proteins which are found in membranes including cellular and intracellular membranes. They consist of two types, peripheral and integral proteins. They include most membrane-associated enzymes, antigenic proteins, transport proteins, and drug, hormone, and lectin receptors.
Theoretical models simulating behavior or activities in nursing, including nursing care, management and economics, theory, assessment, research, and education. Some examples of these models include Orem Self-Care Model, Roy Adaptation Model, and Rogers Life Process Model.
Exotoxins produced by certain strains of streptococci, particularly those of group A (STREPTOCOCCUS PYOGENES), that cause HEMOLYSIS.
Cellular DNA-binding proteins encoded by the c-myc genes. They are normally involved in nucleic acid metabolism and in mediating the cellular response to growth factors. Elevated and deregulated (constitutive) expression of c-myc proteins can cause tumorigenesis.
Infections with bacteria of the genus CAMPYLOBACTER.
Any of the processes by which nuclear, cytoplasmic, or intercellular factors influence the differential control (induction or repression) of gene action at the level of transcription or translation.
Short fragments of DNA or RNA that are used to alter the function of target RNAs or DNAs to which they hybridize.
ISOQUINOLINES with a benzyl substituent.
A prediction of the probable outcome of a disease based on a individual's condition and the usual course of the disease as seen in similar situations.
Transport proteins that carry specific substances in the blood or across cell membranes.
A vocabulary database of universal identifiers for laboratory and clinical test results. Its purpose is to facilitate the exchange and pooling of results for clinical care, outcomes management, and research. It is produced by the Regenstrief Institute. (LOINC and RELMA [Internet]. Indianapolis: The Regenstrief Institute; c1995-2001 [cited 2002 Apr 2]. Available from http://www.regenstrief.org/loinc)
A group of compounds with the heterocyclic ring structure of benzo(c)pyridine. The ring structure is characteristic of the group of opium alkaloids such as papaverine. (From Stedman, 25th ed)
An indolocarbazole that is a potent PROTEIN KINASE C inhibitor which enhances cAMP-mediated responses in human neuroblastoma cells. (Biochem Biophys Res Commun 1995;214(3):1114-20)
Highly crosslinked and insoluble basic anion exchange resin used as anticholesteremic. It may also may reduce triglyceride levels.
Venoms produced by the wasp (Vespid) family of stinging insects, including hornets; the venoms contain enzymes, biogenic amines, histamine releasing factors, kinins, toxic polypeptides, etc., and are similar to bee venoms.
A family of intracellular CYSTEINE ENDOPEPTIDASES that play a role in regulating INFLAMMATION and APOPTOSIS. They specifically cleave peptides at a CYSTEINE amino acid that follows an ASPARTIC ACID residue. Caspases are activated by proteolytic cleavage of a precursor form to yield large and small subunits that form the enzyme. Since the cleavage site within precursors matches the specificity of caspases, sequential activation of precursors by activated caspases can occur.
The return of a sign, symptom, or disease after a remission.
The process of cleaving a chemical compound by the addition of a molecule of water.
A 170-kDa transmembrane glycoprotein from the superfamily of ATP-BINDING CASSETTE TRANSPORTERS. It serves as an ATP-dependent efflux pump for a variety of chemicals, including many ANTINEOPLASTIC AGENTS. Overexpression of this glycoprotein is associated with multidrug resistance (see DRUG RESISTANCE, MULTIPLE).
Period after successful treatment in which there is no appearance of the symptoms or effects of the disease.
A synthetic amino acid that depletes glutathione by irreversibly inhibiting gamma-glutamylcysteine synthetase. Inhibition of this enzyme is a critical step in glutathione biosynthesis. It has been shown to inhibit the proliferative response in human T-lymphocytes and inhibit macrophage activation. (J Biol Chem 1995;270(33):1945-7)

Epstein-barr virus regulates c-MYC, apoptosis, and tumorigenicity in Burkitt lymphoma. (1/3362)

Loss of the Epstein-Barr virus (EBV) genome from Akata Burkitt lymphoma (BL) cells is coincident with a loss of malignant phenotype, despite the fact that Akata and other EBV-positive BL cells express a restricted set of EBV gene products (type I latency) that are not known to overtly affect cell growth. Here we demonstrate that reestablishment of type I latency in EBV-negative Akata cells restores tumorigenicity and that tumorigenic potential correlates with an increased resistance to apoptosis under growth-limiting conditions. The antiapoptotic effect of EBV was associated with a higher level of Bcl-2 expression and an EBV-dependent decrease in steady-state levels of c-MYC protein. Although the EBV EBNA-1 protein is expressed in all EBV-associated tumors and is reported to have oncogenic potential, enforced expression of EBNA-1 alone in EBV-negative Akata cells failed to restore tumorigenicity or EBV-dependent down-regulation of c-MYC. These data provide direct evidence that EBV contributes to the tumorigenic potential of Burkitt lymphoma and suggest a novel model whereby a restricted latency program of EBV promotes B-cell survival, and thus virus persistence within an immune host, by selectively targeting the expression of c-MYC.  (+info)

Reactive oxygen intermediate-dependent NF-kappaB activation by interleukin-1beta requires 5-lipoxygenase or NADPH oxidase activity. (2/3362)

We previously reported that the role of reactive oxygen intermediates (ROIs) in NF-kappaB activation by proinflammatory cytokines was cell specific. However, the sources for ROIs in various cell types are yet to be determined and might include 5-lipoxygenase (5-LOX) and NADPH oxidase. 5-LOX and 5-LOX activating protein (FLAP) are coexpressed in lymphoid cells but not in monocytic or epithelial cells. Stimulation of lymphoid cells with interleukin-1beta (IL-1beta) led to ROI production and NF-kappaB activation, which could both be blocked by antioxidants or FLAP inhibitors, confirming that 5-LOX was the source of ROIs and was required for NF-kappaB activation in these cells. IL-1beta stimulation of epithelial cells did not generate any ROIs and NF-kappaB induction was not influenced by 5-LOX inhibitors. However, reintroduction of a functional 5-LOX system in these cells allowed ROI production and 5-LOX-dependent NF-kappaB activation. In monocytic cells, IL-1beta treatment led to a production of ROIs which is independent of the 5-LOX enzyme but requires the NADPH oxidase activity. This pathway involves the Rac1 and Cdc42 GTPases, two enzymes which are not required for NF-kappaB activation by IL-1beta in epithelial cells. In conclusion, three different cell-specific pathways lead to NF-kappaB activation by IL-1beta: a pathway dependent on ROI production by 5-LOX in lymphoid cells, an ROI- and 5-LOX-independent pathway in epithelial cells, and a pathway requiring ROI production by NADPH oxidase in monocytic cells.  (+info)

Human granulocytic ehrlichiosis agent and Ehrlichia chaffeensis reside in different cytoplasmic compartments in HL-60 cells. (3/3362)

The human granulocytic ehrlichiosis (HGE) agent resides and multiplies exclusively in cytoplasmic vacuoles of granulocytes. Double immunofluorescence labeling was used to characterize the nature of the HGE agent replicative inclusions and to compare them with inclusions containing the human monocytic ehrlichia, Ehrlichia chaffeensis, in HL-60 cells. Although both Ehrlichia spp. can coinfect HL-60 cells, they resided in separate inclusions. Inclusions of both Ehrlichia spp. were not labeled with either anti-lysosome-associated membrane protein 1 or anti-CD63. Accumulation of myeloperoxidase-positive granules were seen around HGE agent inclusions but not around E. chaffeensis inclusions. 3-(2, 4-Dinitroanilino)-3'-amino-N-methyldipropylamine and acridine orange were not localized to either inclusion type. Vacuolar-type H+-ATPase was not colocalized with HGE agent inclusions but was weakly colocalized with E. chaffeensis inclusions. E. chaffeensis inclusions were labeled with the transferrin receptor, early endosomal antigen 1, and rab5, but HGE agent inclusions were not. Some HGE agent and E. chaffeensis inclusions colocalized with major histocompatibility complex class I and II antigens. These two inclusions were not labeled for annexins I, II, IV, and VI; alpha-adaptin; clathrin heavy chain; or beta-coatomer protein. Vesicle-associated membrane protein 2 colocalized to both inclusions. The cation-independent mannose 6-phosphate receptor was not colocalized with either inclusion type. Endogenously synthesized sphingomyelin, from C6-NBD-ceramide, was not incorporated into either inclusion type. Brefeldin A did not affect the growth of either Ehrlichia sp. in HL-60 cells. These results suggest that the HGE agent resides in inclusions which are neither early nor late endosomes and does not fuse with lysosomes or Golgi-derived vesicles, while E. chaffeensis resides in an early endosomal compartment which accumulates the transferrin receptor.  (+info)

A photodynamic pathway to apoptosis and necrosis induced by dimethyl tetrahydroxyhelianthrone and hypericin in leukaemic cells: possible relevance to photodynamic therapy. (4/3362)

The mechanism of cell death induction by dimethyl tetrahydroxyhelianthrone (DTHe), a new second-generation photodynamic sensitizer, is analysed in human leukaemic cell lines in comparison with the structurally related hypericin. DTHe has a broad range of light spectrum absorption that enables effective utilization of polychromatic light. Photosensitization of HL-60 cells with low doses of DTHe (0.65 microM DTHe and 7.2 J cm(-2) light energy) induced rapid apoptosis of > or =90% of the cells. At doses > or =2 microM, dying cells assumed morphological necrosis with perinucleolar condensation of chromatin in HL-60 and K-562 cell lines. Although nuclear fragmentation that is characteristic to apoptosis was prevented, DNA digestion to oligonucleosomes proceeded unhindered. Such incomplete apoptosis was more prevalent with the related analogue hypericin throughout most doses of photosensitization. Despite hypericin being a stronger photosensitizer, DTHe exhibited advantageous phototoxic properties to tumour cells, initiating apoptosis at concentrations about threefold lower than hypericin. Photosensitization of the cells induced dissociation of the nuclear envelope, releasing lamins into the cytosol. DTHe also differed from hypericin in effects exerted on the nuclear lamina, causing release of an 86-kDa lamin protein into the cytosol that was unique to DTHe. Within the nucleus, nuclear envelope lamin B underwent covalent polymerization, which did not affect apoptotic nuclear fragmentation at low doses of DTHe. At higher doses, polymerization may have been extensive enough to prevent nuclear collapse. Hut-78, CD4+ cells were resistant to the photodynamically activated apoptotic pathway. Beyond the tolerated levels of photodynamic damage, these cells died exclusively via necrosis. Hut-78 cells overexpress Bcl-X(L) as well as a truncated Bcl-X(L)tr isoform that could contribute to the observed resistance to apoptosis.  (+info)

Heparin-binding epidermal growth factor-like growth factor/diphtheria toxin receptor expression by acute myeloid leukemia cells. (5/3362)

Heparin-binding epidermal growth factor-like growth factor (HB-EGF) is an EGF family member expressed by numerous cell types that binds to EGF receptor 1 (HER-1) or 4 (HER-4) inducing mitogenic and/or chemotactic activities. Membrane-bound HB-EGF retains growth activity and adhesion capabilities and the unique property of being the receptor for diphtheria toxin (DT). The interest in studying HB-EGF in acute leukemia stems from these mitogenic, chemotactic, and receptor functions. We analyzed the expression of HB-EGF in L428, Raji, Jurkat, Karpas 299, L540, 2C8, HL-60, U937, THP-1, ML-3, and K562 cell lines and in primary blasts from 12 acute myeloid leukemia (AML) cases, by reverse-transcriptase polymerase chain reaction (RT-PCR) and Northern blot and by the evaluation of sensitivity to DT. The release of functional HB-EGF was assessed by evaluation of its proliferative effects on the HB-EGF-sensitive Balb/c 3T3 cell line. HB-EGF was expressed by all myeloid and T, but not B (L428, Raji), lymphoid cell lines tested, as well as by the majority (8 of 12) of ex vivo AML blasts. Cell lines (except for the K562 cell line) and AML blasts expressing HB-EGF mRNA underwent apoptotic death following exposure to DT, thus demonstrating the presence of the HB-EGF molecule on their membrane. Leukemic cells also released a fully functional HB-EGF molecule that was mitogenic for the Balb/c 3T3 cell line. Factors relevant to the biology of leukemic growth, such as tumor necrosis factor-alpha (TNF-alpha), 1alpha,25-(OH)2D3, and especially all-trans retinoic acid (ATRA), upregulated HB-EGF mRNA in HL-60 or ML-3 cells. Granulocyte-macrophage colony-stimulating factor (GM-CSF) induced HB-EGF mRNA and acquisition of sensitivity to DT in one previously HB-EGF-negative leukemia case. Moreover, the U937 and Karpas 299 cell lines expressed HER-4 mRNA. This work shows that HB-EGF is a growth factor produced by primary leukemic cells and regulated by ATRA, 1alpha, 25-(OH)2D3, and GM-CSF.  (+info)

Interaction of 5-lipoxygenase with cellular proteins. (6/3362)

5-Lipoxygenase (5LO) plays a pivotal role in cellular leukotriene synthesis. To identify proteins interacting with human 5LO, we used a two-hybrid approach to screen a human lung cDNA library. From a total of 1.5 x 10(7) yeast transformants, nine independent clones representing three different proteins were isolated and found to specifically interact with 5LO. Four 1.7- to 1.8-kb clones represented a 16-kDa protein named coactosin-like protein for its significant homology with coactosin, a protein found to be associated with actin in Dictyostelium discoideum. Coactosin-like protein thus may provide a link between 5LO and the cytoskeleton. Two other yeast clones of 1.5 kb encoded transforming growth factor (TGF) type beta receptor-I-associated protein 1 partial cDNA. TGF type beta receptor-I-associated protein 1 recently has been reported to associate with the activated form of the TGF beta receptor I and may be involved in the TGF beta-induced up-regulation of 5LO expression and activity observed in HL-60 and Mono Mac 6 cells. Finally, three identical 2.1-kb clones contained the partial cDNA of a human protein with high homology to a hypothetical helicase K12H4. 8 from Caenorhabditis elegans and consequently was named DeltaK12H4. 8 homologue. Analysis of the predicted amino acid sequence revealed the presence of a RNase III motif and a double-stranded RNA binding domain, indicative of a protein of nuclear origin. The identification of these 5LO-interacting proteins provides additional approaches to studies of the cellular functions of 5LO.  (+info)

Bcl-2 alters the balance between apoptosis and necrosis, but does not prevent cell death induced by oxidized low density lipoproteins. (7/3362)

Oxidized low density lipoproteins (oxLDL) participate in atherosclerosis plaque formation, rupture, and subsequent thrombosis. Because oxLDL are toxic to cultured cells and Bcl-2 protein prevents apoptosis, the present work aimed to study whether Bcl-2 may counterbalance the toxicity of oxLDL. Two experimental model systems were used in which Bcl-2 levels were modulated: 1) lymphocytes in which the (high) basal level of Bcl-2 was reduced by antisense oligonucleotides; 2) HL60 and HL60/B (transduced by Bcl-2) expressing low and high Bcl-2 levels, respectively. In cells expressing relatively high Bcl-2 levels (lymphocytes and HL60/B), oxLDL induced mainly primary necrosis. In cells expressing low Bcl-2 levels (antisense-treated lymphocytes, HL60 and ECV-304 endothelial cells), the rate of oxLDL-induced apoptosis was higher than that of primary necrosis. OxLDL evoked a sustained calcium rise, which is a common trigger to necrosis and apoptosis since both types of cell death were blocked by the calcium chelator EGTA. Conversely, a sustained calcium influx elicited by the calcium ionophore A23187 induced necrosis in cells expressing high Bcl-2 levels and apoptosis in cells expressing low Bcl-2 levels. This suggests that Bcl-2 acts downstream from the calcium peak and inhibits only the apoptotic pathway, not the necrosis pathway, thus explaining the apparent shift from oxLDL-induced apoptosis toward necrosis when Bcl-2 is overexpressed.  (+info)

Effects of novel RAR- and RXR-selective retinoids on myeloid leukemic proliferation and differentiation in vitro. (8/3362)

Retinoids such as all-trans-retinoic acid (ATRA) and 9-cis-retinoic acid (9-cis-RA) have an important role in many aspects of proliferation and differentiation of hematopoietic cells. They exert their effects by binding to retinoic acid receptors (RARs) and/or retinoid X receptors (RXRs). We studied the effects of novel retinoids on proliferation and differentiation of HL-60 and NB4 myeloid leukemic cells, as well as acute promyelocytic leukemia (APL) cells from patients. RXR-selective SR11345 (Retinoid C) had little ability to inhibit the clonal growth and to induce the differentiation of either HL-60 or NB4 cells. However, SR11276 (Retinoid E), which activated both the RAR and RXR classes, and SR11278 (Retinoid D), which activated the RAR subtypes alpha, beta, and gamma, could inhibit clonal growth of both cell types, as well as leukemic cells from APL patients. The combination of ATRA and either SR11276 or SR11278 additively inhibited APL cell proliferation. SR11302 (Retinoid A), with reported anti-AP-1 activity and no activation of RARs and RXR and SR11363 (Retinoid B), which selectively activated RARbeta and gamma, were inactive. The clonal proliferation of both HL-60 and NB4 cells that were pulse-exposed to 10(-9) mol/L ATRA, SR11276, SR11278, or SR11345 for 3 days, washed, and plated in methylcellulose culture were inhibited by 0%, 51%, 21%, and 1% for HL-60 cells and 43%, 41%, 35%, and 1% for NB4, respectively, compared with nontreated control cells. When the HL-60 cells were pulse-exposed to 10(-9) mol/L of either SR11278 or SR11276, plus 10(-9) mol/L ATRA for 3 days, colony numbers were reduced by 46% and 64%, respectively. Induction of leukemic cell differentiation as determined by the nitroblue tetrazolium (NBT) assay showed that the combination of 10(-7) mol/L of either SR11278 or SR11276 with 10(-7) mol/L ATRA had additive effects on HL-60 cells, NB4 cells, and fresh APL cells. Induction of CD11b expression on both HL-60 and NB4 cells occurs during their differentiation. Expression of this antigen was synergistically augmented by the combination of either 10(-7) to 10(-8) mol/L SR11278 or 10(-7) to 10(-9) mol/L SR11276 with 10(-9) mol/L ATRA compared with either analog alone in HL-60 cells. Expression of the novel myeloid specific transcription factor C/EBPepsilon was increased by SR11278 and SR11276 in both the HL-60 and NB4 cell lines. We conclude that retinoids or combination of retinoids with specificities for both RAR and RXR may markedly enhance the ability of ATRA to inhibit clonal growth and induce differentiation of HL-60 and NB4 leukemic cells. This occurs in the absence of continuous contact with retinoids.  (+info)

BioAssay record AID 80927 submitted by ChEMBL: Compound was tested for differentiation-inducing activity against human promyelocytic leukemia cell line HL-60.
Hypoxia is known to regulate the expression of genes involved in the migration of various cell types. Although many studies have shown that hypoxia increases cell migration, it still remains unclear whether hypoxia could modulate the stromal cell derived factor-1 (SDF-1)-dependent migration of leukemic cell. Herein, we demonstrated that the SDF-1-dependent migration of HL-60, was reduced under hypoxia with no comparable decrease of CXC-type chemokine receptor CXCR4, a cognate receptor for SDF-1. Furthermore, we showed that migration toward SDF-1 was reduced by inactivation of either serine/threonine kinase Akt or extracellular signal regulated kinase Erk, which was confirmed by selective pathway inhibitor LY294002 and PD98059. In our results, phosphorylation of Erk was increased under hypoxia, but phosphorylation of Akt was attenuated on the contrary. These results led us to conclusion that hypoxia could inhibit the SDF-1-dependent migration of HL-60 via blocking of Akt activation ...
PubMed comprises more than 30 million citations for biomedical literature from MEDLINE, life science journals, and online books. Citations may include links to full-text content from PubMed Central and publisher web sites.
Journal of Unexplored Medical Data is an online journal that encompasses reporting of pilot and unpublished clinical and biological medical studies.
Differentiation therapy in the treatment of leukemia is often hampered by limitations on using certain pharmaceutical regents or on the required doses due to various reasons, such as drug-resistance and retinoic acid syndrome. To circumvent these problems, a strategy might be developed on the basis of the ability of drug-differentiated cells to stimulate differentiation in leukemia cells. Using the promyelocytic leukemia cell line HL60 as a cell model, we assessed the differentiation-stimulating potency of differentiated granulocytes and monocytes/macrophages after treatments with all-trans retinoic acid (ATRA) and 12-O-tetradecanoylphorbol-13-acetate (TPA), respectively. ATRA- and TPA-differentiated cells were able to stimulate differentiation in fresh HL60 cells, accompanied by inhibition on cell growth to various extents. The differentiated cells of the second generation, especially those originated from TPA treatment, were as potent as the drugs themselves in stimulating differentiation in ...
The Ins(1,4,5)P3 receptor was examined in human promyelocytic leukaemic cells (HL-60) and in HL-60 cells differentiated towards granulocytes with either retinoic acid (RA) or dimethyl sulphoxide (Me2SO). HL-60 cell membranes enriched in marker enzyme activities of the endoplasmic reticulum and the plasma membrane possess a high-affinity binding site for [3H]Ins(1,4,5)P3 (KD = 22 nM). Electrotransfer studies indicate that Ins(1,4,[32P]5)P3 binds specifically to a 260 kDa protein of HL-60 cell membranes. This Ins(1,4,5)P3-binding protein selectively binds Ca(2+)-mobilizing inositol phosphates and other inositol phosphates which also bind to the purified InsP3 receptor, suggesting that the Ins(1,4,5)P3-binding protein of HL-60 cell membranes is the InsP3 receptor. When HL-60 cells are incubated with 1 microM-RA or with 1.25% Me2SO the cells differentiate within 5-7 days into cells resembling neutrophils in both structure and function. Treated cells cease to proliferate, acquire the ability to ...
Evidence-Based Complementary and Alternative Medicine (eCAM) is an international peer-reviewed, Open Access journal that seeks to understand the sources and to encourage rigorous research in this new, yet ancient world of complementary and alternative medicine.
TY - JOUR. T1 - Decomposition of gene expression state space trajectories. AU - Mar, Jessica C.. AU - Quackenbush, John. PY - 2009/12/1. Y1 - 2009/12/1. N2 - Representing and analyzing complex networks remains a roadblock to creating dynamic network models of biological processes and pathways. The study of cell fate transitions can reveal much about the transcriptional regulatory programs that underlie these phenotypic changes and give rise to the coordinated patterns in expression changes that we observe. The application of gene expression state space trajectories to capture cell fate transitions at the genome-wide level is one approach currently used in the literature. In this paper, we analyze the gene expression dataset of Huang et al. (2005) which follows the differentiation of promyelocytes into neutrophil-like cells in the presence of inducers dimethyl sulfoxide and all-trans retinoic acid. Huang et al. (2005) build on the work of Kauffman (2004) who raised the attractor hypothesis, ...
LED Advanced Flash Nipple (HL-145) - Browse fuzing.com to find LED Advanced Flash Nipple (HL-145) sellers, suppliers, wholesalers, companies, manufacturers, exporters, factories.
BioAssay record AID 81650 submitted by ChEMBL: Compound at 100 uM was tested in vitro to inhibit proliferation of HL-60 leukemia cell line.
in rodents and primates of 50 to 60% and a serum half-life of 5 to 6 hours. . was always found on a CCR5P1-bearing haplotype and that CCR5-delta-32 was .. CCL5 -403A without the 32-bp deletion in CCR5 was not associated with HBV. ...
Unit 1: Cell Biology Introduction to Cells Outline: Cell Theory 1. All living things are made of cells which: are surround by a membrane contain genetic...
TY - JOUR. T1 - Separation and analysis of subcellular organelles in a human promyelocytic leukemia cell line, HL-60. T2 - Application to the study of myeloid lysosomal enzyme synthesis and processing. AU - Nauseef, W. M.. AU - Clark, Robert A. PY - 1986. Y1 - 1986. N2 - We describe a system for analysis of the intracellular pathways in the biosynthesis and packaging of functionally important proteins in human myeloid cells. The human promyelocytic cell line HL-60 was used since peripheral blood neutrophils are terminally differentiated and do not actively synthesize protein. Cells were disrupted by nitrogen cavitation and subcellular organelles in postnuclear supernatant separated on a discontinuous gradient of Percoll modified to resolve organelles important in protein synthesis. This Percoll gradient separated azurophilic granules from less dense organelles and partially separated the less dense organelles from one another. Approximate densities of organelles identified by electron microscopy ...
The present study selected and characterized a multidrug‑resistant HL‑60 human acute promyelocytic leukemia cell line, HL‑60/RS, by exposure to stepwise incremental doses of doxorubicin. The drug‑resistant HL‑60/RS cells exhibited 85.68‑fold resistance to doxorubicin and were cross‑resistant to other chemotherapeutics, including cisplatin, daunorubicin, cytarabine, vincristine and etoposide. The cells over‑expressed the transporters P‑glycoprotein, multidrug‑resistance‑related protein 1 and breast‑cancer‑resistance protein, encoded by the adenosine triphosphate‑binding cassette (ABC)B1, ABCC1 and ABCG2 genes, respectively. Unlike other recognized chemoresistant leukemia cell lines, HL‑60/RS cells were also strongly cross‑resistant to arsenic trioxide. The proportion of leukemia stem cells (LSCs) increased synchronously with increased of drug resistance in the doxorubicin‑induced HL‑60 cell population. The present study confirmed that doxorubicin‑induced ...
TY - JOUR AU - Jakovljević, Katarina AU - Joksovic, Milan D. AU - Matić, Ivana Z. AU - Petrovic, Nina AU - Stanojković, Tatjana AU - Sladić, Dušan AU - Vujčić, Miroslava AU - Janović, Barbara AU - Joksovic, Ljubinka AU - Trifunović, Snežana AU - Markovic, Violeta PY - 2018 UR - http://cer.ihtm.bg.ac.rs/handle/123456789/2379 AB - Hybrid compounds that combine the 1,3,4-thiadiazole-containing catechol moiety with a chalcone motif were synthesized and examined for their antioxidant activity, cytotoxicity, and DNA-binding activity. A series of thirteen compounds showed strong antioxidant and cytotoxic effects on human acute promyelocytic leukemia HL-60 cells. Several compounds exerted good cytotoxic activities on cervical adenocarcinoma HeLa cells. The treatment of HeLa cells with IC50 and double IC50 concentrations of the compounds 5a, 5c, 5f, and 5m induced a statistically significant increase in the percentage of cells within a subG1 cell cycle phase. The examined compounds caused G2/M ...
TY - JOUR. T1 - Analysis of gene profiles involved in the enhancement of all-trans retinoic acid-induced HL-60 cell differentiation by sesquiterpene lactones identifies asparagine synthetase as a novel target for differentiation-inducing therapy. AU - Song, Ju Han. AU - Kim, Seung Hyun. AU - Cho, Kyung Min. AU - Hwang, Seung Yong. AU - Kim, Hyeoung Joon. AU - Kim, Tae Sung. PY - 2014/3/1. Y1 - 2014/3/1. N2 - All-trans retinoic acid (ATRA) is one of the most useful drugs in the treatment for acute promyelocytic leukemia (APL), but its adverse effects, which include drug resistance and hypercalcemia are obstacles to achieving complete remission. Our previous study showed that some sesquiterpene lactones (STLs), i.e., helenalin (HE) and parthenolide (PA) but not sclareolide (SC), enhance ATRA-induced differentiation of HL-60 APL cells with no unexpected effects, but the precise mechanism on underlying this synergism is not yet fully understood. In this study, we investigated the distinctive ...
The mechanism of neutrophil activation by the chemotactic peptide formyl-methionyl-leucyl-phenylalanine (FMLP) has been studied by pretreatment of human neutrophils with pertussis toxin. Upon stimulation with FMLP, the cytosolic-free calcium concentration, [Ca2+]i, is increased both by stimulation of calcium influx and mobilization of cellular calcium. We have measured [Ca2+]i as well as the generation of the phospholipid breakdown product inositol trisphosphate (IP3), which is thought to mediate Ca2+ mobilization. As the phosphoinositide pool in human neutrophils is difficult to prelabel with [3H]myoinositol, experiments were also carried out in the cultured human promyelocytic leukemia cell line HL-60 after differentiation with dimethylsulfoxide. Pertussis toxin pretreatment of both cell types inhibited FMLP stimulated membrane depolarization, exocytosis, and superoxide production in a dose-dependent manner. This toxin effect was selective for the receptor agonist, since stimulation of these ...
Anti-leukemic activity of phosphoproteins from Sesamin via induction of nuclear antigen H731and CLIP-associating protein 2 isoform X25 mediated apoptosis
TC Hsieh, J Kunichki, Z Darzynkiewicz, JM Wu.. Department of Biochemistry and Molecular Biology, New York Medical College, Valhalla, NY, USA.. OBJECTIVE: The goal of this in vitro study was to test the cytostatic and cytotoxic activities of extracts derived from the polysaccharopeptide (PSP), Im-Yunity (Integrated Chinese Medicine Holdings Ltd., Kowloon, Hong Kong) prepared from strain Cov-1 of the mushroom Coriolus versicolor. DESIGN: Different volumes of 70% ethanol and water extracts of Im-Yunity were incubated with cultures of human promyelocytic leukemic HL-60 cells, and compared to nontreated control cells. At various times after treatment, cells were harvested and analyzed with respect to: (1). proliferation and cell cycle phase distribution, (2). induction of apoptosis, and (3). changes in expression of the immunomodulating cytokines interleukin (IL)-1 beta, IL-6, and IL-8. To test whether extracts also affected normal cells, similar experiments were also performed using isolated ...
Treatment of intact NIH 3T3 cells with 12-O-tetradecanoylphorbol-13-acetate (TPA) causes a rapid redistribution (stabilization) of protein kinase C to the particulate fraction. Part of the enzyme activity stabilized to the membrane fraction in response to TPA can be recovered associated with nuclear-cytoskeletal components. An apparently pure nuclear fraction prepared from NIH 3T3 cells was found to contain 25-30% of the total membrane-associated protein kinase C activity when isolated in the presence of Ca2+. In untreated control cells, most of this activity found with the nuclear fraction can be extracted by chelators. Phorbol easter (TPA) treatment of NIH 3T3 cells induces the tight association of protein kinase C to the nucleus; this tightly bound activity is not dissociable by chelators and can be recovered only by solubilization with detergent. Nuclei purified from untreated human promyelocytic leukemic HL-60 cells contain higher amounts of chelator-stable, detergent-extractable protein ...
Sigma-Aldrich offers abstracts and full-text articles by [Elisabetta Cavalieri, Antonella Rigo, Massimiliano Bonifacio, Alessandra Carcereri de Prati, Emanuele Guardalben, Christian Bergamini, Romana Fato, Giovanni Pizzolo, Hisanori Suzuki, Fabrizio Vinante].
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TY - JOUR. T1 - Soluble CD141-152 confers responsiveness to both lipoarabinomannan and lipopolysaccharide in a novel HL-60 cell bioassay. AU - Yu, Weiming. AU - Soprana, Elisa. AU - Cosentino, Giovanna. AU - Volta, Manuela. AU - Lichenstein, Henri S.. AU - Viale, Giovanna. AU - Vercelli, Donata. PY - 1998/10/15. Y1 - 1998/10/15. N2 - CD14 is a pattern recognition receptor involved in the interaction with multiple ligands, including LPS from Gram-negative bacteria and lipoarabinomannan (LAM) from mycobacteria. While the interactions between LPS and soluble CD14 (sCD14) have been analyzed in detail, LAM/CD14 interactions remain uncharacterized due to the lack of suitable functional assays. We describe herein a novel bioassay for the analysis of CD14/ligand interactions. CD14-negative myeloid HL-60 cells up-regulate endogenous CD14 gene expression when stimulated with LPS in the presence of recombinant soluble CD141-348. Using the HL-60 bioassay, we showed that sCD141- 348 confers responsiveness ...
While the molecular and biophysical mechanisms underlying cell protrusion on two-dimensional substrates are well understood, our knowledge of the actin structures driving protrusion in three-dimensional environments is poor, despite relevance to inflammation, development and cancer. Here we report that, during chemotactic migration through microchannels with 5 μm × 5 μm cross-sections, HL60 neutrophil-like cells assemble an actin-rich slab filling the whole channel cross-section at their front. This leading edge comprises two distinct F-actin networks: an adherent network that polymerizes perpendicular to cell-wall interfaces and a free network that grows from the free membrane at the cell front. Each network is polymerized by a distinct nucleator and, due to their geometrical arrangement, the networks interact mechanically. On the basis of our experimental data, we propose that, during interstitial migration, medial growth of the adherent network compresses the free network preventing its
HL-60 is a promyelocytic cell line derived by S.J. Collins, et al. Peripheral blood leukocytes were obtained by leukopheresis from a 36-year-old Caucasian female with acute promyelocytic leukemia.
Our findings suggest an inherent, cryptic chirality in VMCs that is revealed by an unbiased extracellular mechanical transition and mediated by cytoskeletal reorganization, analogous to chemically induced chirality seen in neutrophil-like cells.31 To our knowledge, this is the first demonstration of an association between LR asymmetry and cytoskeletal reorganization, triggered by an unbiased mechanical interface, and the first demonstration that a microscale dynamic asymmetry unfolds into a de novo, consistently oriented and periodic macroscale pattern resembling tissue architecture. In VMCs, the rightward-biased turning required stress-fiber accumulation at the FN/PEG interface, suggesting that chirality may be in the architecture of the actin filament assembly at the macroscale level, say as clockwise or counterclockwise orientation. Alternatively, it may arise from chirality at the micro- or nanoscale, such as helicity of microfilaments, or chiral rotagen molecules, such as dynein or myosin, ...
Cardiac structure and functionare commonly studied using primary culture of neonatal and adult cardiac myocyte. However, their inability to divide and retain their differentiated phenotype in culture limits their use.. Established from a mouse atrial myocyte tumour, HL-1 cells share similar characteristics with primary cultures of cardiac myocytes. They have the ability to proliferate while keeping a differentiated cardiomyocyte phenotype in culture (this allows the use of specific molecular tools as RNA interference). However, there are concerns about their genetic stability and some studies have shown the cells to contain a functionally heterogeneous population.. The team from Imperial College isolated homogeneous and stable clones of HL-1 cell lines - thereby excluding any differences due to cellular heterogeneity of the original cell line - that display phenotypic characteristics consistent with cardiac cells.. Clones 3 and 6 appear to be most promising for cardiac research. These cells ...
Janet E. Rubin (jrubi02 at unix.cc.emory.edu) wrote: : Can anyone help us with our RT-PCR problem? We have GAP : primers that work perfectly well in rat and mouse - and in fact were : suggested by a BioTechniques article because they were : supposed to read human as well. However, despite being able : to RT-PCR other things from HL-60 cells, our GAP primers do not : work, at all (no band). So the question is, is GAP wierd in HL-60 : cells (has anyone else RTd GAP without problems?), any : suggestions for good primers for housekeeping-ish genes in this : cell line? : Thanks in advance (or previously as someone once wrote). -- Shahram Mori _/\_ Program in Molecular Biology _\ /_ Dept. of chemistry and Biochemistry Box 3C \_ _/ NMSU Las Cruces NM ...
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Detailed information about the celline expression of DLST in HL-60 stained with HPA003010. The antibody showed a High level of staining
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In these studies we have identified a novel small molecular weight mimetic of TGF-β, A-161906. A-161906 was originally synthesized as part of a series of metalloproteinase inhibitors at Abbott Laboratories. A-161906 is a micromolar inhibitor of TNF-α release in PMA-stimulated HL-60 cells. PMA stimulates the release of TNF-α concomitant with the differentiation of HL-60 cells. However, in a lipopolysaccharide-stimulated model in a more monocytic-differentiated THP-1 cell line, A-161906 has no effect on TNF-α release. This suggested that A-161906 had other mechanisms aside from the inhibition of TNF-α release, a metalloproteinase-dependent process, that were associated with the differentiation of HL-60 cells by PMA. HTS of a PAI-1/luciferase construct in Mv1Lu cells provided the identification of a novel activity of A-161906, modulation of transcriptional promoter activity in a cellular assay sensitive to TGF-β (32).. In cancer and other diseases where the normal responses to TGF-β have ...
TY - JOUR. T1 - Ellagitannins from Terminalia calamansanai induced apoptosis in HL-60 cells. AU - Chen, Lih Geeng. AU - Huang, Wen Tsung. AU - Lee, Lain Tze. AU - Wang, Ching Chiung. PY - 2009/6. Y1 - 2009/6. N2 - Terminalia calamansanai (Blanco) Rolf. (Combretaceae) is used medicinally as lithontriptic in Philippines. The 70% acetone extracts of T. calamansanai leaves inhibited the viability of human promyelocytic leukemia HL-60 cells. 1-α-O-Galloylpunicalagin, punicalagin, 2-O-galloylpunicalin, sanguiin H-4, and methyl gallate were the main components isolated from T. calamansanai with the IC 50 values of 65.2, 74.8, 42.2, 38.0 and ,100 μM, respectively, for HL-60 cells. Apoptosis of HL-60 cells treated with 1-α-O-galloylpunicalagin, punicalagin, 2-O-galloylpunicalin, and sanguiin H-4 was noted by the appearance of a sub-G 1 peak in flow cytometric analysis and DNA fragmentation by gel electrophoresis. 2-O-Galloylpunicalin and sanguiin H-4 induced a decrease of the human ...
TY - JOUR. T1 - Dysregulated bcl-2 expression inhibits apoptosis but not differentiation of retinoic acid-induced HL-60 granulocytes. AU - Park, Ulie R.. AU - Robertson, Kent. AU - Hickstein, Dennis D.. AU - Tsai, Schickwann. AU - Hockenbery, David M.. AU - Collins, Steven J.. N1 - Copyright: Copyright 2020 Elsevier B.V., All rights reserved.. PY - 1994/7/15. Y1 - 1994/7/15. N2 - The bcl-2 proto-oncogene appears to contribute to the development of certain malignancies by inhibiting programmed cell death (apoptosis). Mature granulocytes show a markedly limited life span and rapidly undergo apoptosis. To further define the relationship between apoptosis and granulocyte differentiation, we used retroviral vector-mediated gene transduction to introduce the normal bcl-2 gene into the HL-60 myeloid leukemia cell line and determined the response of these bcl-2-transduced HL-60 cells to the induction of granulocyte differentiation by retinoic acid (RA). Although the bcl-2-transduced HL-60 cells showed ...
Mitogen-activated protein kinases (MAPKs) are important transducers of external signals for cell growth, survival, and other cellular responses including cell differentiation. Several MAPK cascades are known with the MEK1/2-ERK1/2, JNK, and p38MAPKs receiving most attention, but the role of MEK5-ERK5 in intracellular signaling deserves more scrutiny, as this pathway transmits signals that can complement ERK/2 signaling. We hypothesized that the ERK5 pathway plays a role in the control of monocytic differentiation, which is disturbed in myeloid leukemia. We therefore examined the cellular phenotype and key molecular events which occur when human myeloid leukemia cells, acute (AML) or chronic (CML), are forced to differentiate by vitamin D derivatives (VDDs). This study was performed using established cell lines HL60 and U937, and primary cultures of blasts from 10 patients with ML. We found that ERK5 and its direct downstream target transcription factor MEF2C are upregulated by 1,25D in parallel ...
The HL-60 (Human promyelocytic leukemia cells) cell line has been used for laboratory research on how certain kinds of blood cells are formed. HL-60 proliferates continuously in suspension culture in nutrient and antibiotic chemicals. The doubling time is about 36-48 hours. The cell line was derived from a 36-year-old woman with acute promyelocytic leukemia at MD Anderson Cancer Center. HL-60 cells are predominantly a neutrophilic promyelocyte (precursor). Proliferation of HL-60 cells occurs through the transferrin and insulin receptors, which are expressed on cell surface. The requirement for insulin and transferrin is absolute, as HL-60 proliferation immediately ceases if either of these compounds is removed from the serum-free culture media. With this line, differentiation to mature granulocytes can be induced by compounds such as dimethyl sulfoxide (DMSO), or retinoic acid. Other compounds like 1,25-dihydroxyvitamin D3, 12-O-tetradecanoylphorbol-13-acetate (TPA) and GM-CSF can induce HL-60 ...
Ultraviolet B (UVB) radiation acts as a strong apoptotic trigger in many cell types, in tumor and normal cells. Several studies have demonstrated that UVB-induced cell death occurs through the generation of reactive oxygen species. The consequent oxidative stress includes the impairment of cellular antioxidants, the induction of DNA damage and the occurrence of apoptosis. In this review, we investigated UVB apoptotic action in various cell models by using ultrastructural, molecular and cytofluorimetric techniques. Myeloid leukemia HL-60, T-lymphoblastoid Molt-4 and myelomonocytic U937 human cells, generally affected by apoptotic stimuli, were studied. Human chondrocytes and C2C12 skeletal muscle cells, known to be more resistant to damage, were also considered. All of them, when exposed to UVB radiation, revealed a number of characteristic apoptotic markers. Membrane blebbing, cytoplasm shrinkage and chromatin condensation were detected by means of electron microscopy. DNA cleavage, investigated by
The cytotoxic response of several types of neoplastic cells to analogues of unnatural alkyl phospholipids (e.g., rac-1-hexadecyl-2-methoxy-glycero-3-phosphocholine) has been partially attributed to their accumulation as a result of the low activity of the alkyl cleavage enzyme (a tetrahydropteridine-dependent monooxygenase) in tumor cells. We tested this possibility by comparing the alkyl cleavage enzyme activity in cells that exhibit differences in sensitivity toward the cytotoxic effects of the rac-1-hexadecyl-2-methoxy-glycero-3-phosphocholine. Human promyelocytic leukemia cells (HL-60), a cell line highly sensitive to the cytotoxic alkyl phospholipid analogue, possessed an alkyl cleavage enzyme activity (0.25 pmol/min/µg protein) similar to that found in three cell types known to be relatively resistant to the cytotoxic activity of the analogue: immature human promyeloblastic leukemia cells (K562) (0.22 pmol/min/µg protein), human polymorphonuclear neutrophils (0.34 pmol/min/µg protein), ...
Ortho-topolin riboside induced cell apoptosis through ERS pathway and inhibited DNMT1 activity in acute myeloid leukemia cells, Li Wang, YanHong Zhao, Jiao Cheng, FanLin Lin, YingY
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CD109 (Cluster of Differentiation 109) is a cell surface antigen that is linked to glycosyl-phosphatidyl-inositol (GPI). CD109 is also known as platelet-specific Gov antigen, 150 kDa TGF-beta-1-binding protein, C3 and PZP-like alpha-2-macroglobulin domain-containing protein 7, CPAMD7, p180, r150, DKFZp762L1111, FLJ38569, and RP11-525G3.1. It is expressed by CD34+ acute myeloid leukemia cell lines, T-cell lines, activated T lymphoblasts, endothelial cells, and activated platelets. CD109 is considered to be a marker of early-stage megakaryocytic hematopoiesis. Overexpression of CD109 has been reported in squamous cell carcinomas, such as lung carcinoma, esophageal carcinoma, and cervical carcinoma.. ...
CD109 (Cluster of Differentiation 109) is a cell surface antigen that is linked to glycosyl-phosphatidyl-inositol (GPI). CD109 is also known as platelet-specific Gov antigen, 150 kDa TGF-beta-1-binding protein, C3 and PZP-like alpha-2-macroglobulin domain-containing protein 7, CPAMD7, p180, r150, DKFZp762L1111, FLJ38569, and RP11-525G3.1. It is expressed by CD34+ acute myeloid leukemia cell lines, T-cell lines, activated T lymphoblasts, endothelial cells, and activated platelets. CD109 is considered to be a marker of early-stage megakaryocytic hematopoiesis. Overexpression of CD109 has been reported in squamous cell carcinomas, such as lung carcinoma, esophageal carcinoma, and cervical carcinoma.. ...
During 1.25% dimethylsulfoxide (DMSO)-triggered granulocytic differentiation of HL-60 cells, the neurotensin-induced [Ca2+]i rise became gradually smaller and completely disappeared 4 days after treatment with DMSO. The mRNA level for neurotensin receptors was also decreased after differentiation ...
We first tested no matter whether unpolarized HL-60 neutrophils can break their morphological symmetry and start off to migrate when they are stimulated by a
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M induced an inhibitory effect against the proliferation of HL-60 and colony potential of HCT-116 cells. The apoptosis in HL-60 cells was associated with down-regulation of Bcl-2 and activation of Bax, while in HCT-116 cells, necrotic features were observed; size of cells was dramatically increased by swelling of cytoplasm and loss of membrane integrity, cell rupture and release of cellular contents. ...
Activation of ERK signaling may promote cardioprotection from ischemia-reperfusion (I/R) injury. ZnT-1, a protein that confers resistance from zinc toxicity, was found to interact with Raf-1 kinase through its C-terminal domain, leading to downstream activation of ERK. In the present study, we evaluated the effects of ZnT-1 in c...Read More ...
The Baseline and Classical controllers use a classic cascaded-loop architecture with three inner P-only loops to control the angular rates p,q,r, and three outer PI loops to control the angular positions phi,alpha,beta. The six proportional gains and three integral gains are all scheduled as a function of alpha and beta. The Baseline variant contains the baseline design featured in docid:aeroblks_ug.f4-50264. Parts 2 and 3 of this series use the Classical variant to walk through the tuning process. The active variant is controlled by the workspace variable CTYPE. Set its value to 2 to activate the Classical variant of the controller.. ...
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Effects of DCA treatment on p53mutated/ leukemic cell linesThe p53mutated B leukemic cell lines MAVER, MEC-1 and MEC-2, as well as the p53 HL-60 cells, were exp
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CGP049090: an inhibitor of WNT signaling, effectively induce apoptosis in acute myeloid leukemia cells; structure in first source
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Cell. Biol. 20 (9): 3157-67. doi:10.1128/MCB.20.9.3157-3167.2000. PMC 85610. PMID 10757800. Zhang QH, Ye M, Wu XY, Ren SX, Zhao ... Datta PK, Moses HL (2000). "STRAP and Smad7 synergize in the inhibition of transforming growth factor beta signaling". Mol. ... Cell. Biol. 20 (9): 3157-67. doi:10.1128/mcb.20.9.3157-3167.2000. PMC 85610. PMID 10757800. Datta PK, Chytil A, Gorska AE, ... Datta PK, Chytil A, Gorska AE, Moses HL (1998). "Identification of STRAP, a novel WD domain protein in transforming growth ...
New York: McGraw-Hill. DeBlase, A., R. Martin. 1981. A Manual of Mammalogy. New York: McGraw-Hill. Nowak, R. 1999. Walker's ... Cells Tissues Organs, 170(1): 36-60. Feldhamer, G., L. Drickamer, S. Vessey, J. Merritt. 1999. Mammalogy. ...
Accordingly, the human leukocyte cell line, HL-60 promyelocytes (which do not respond to FMLP), was purposely differentiated to ... and immature dendritic cells; vascular endothelial cells; neural tissue glial cells, astrocytes, and neuroblastoma cells; liver ... lymphocyte T cells and B cells; tissue Mast cells, macrophages, fibroblasts, ... Murphy PM, Gallin EK, Tiffany HL, Malech HL (Feb 1990). "The formyl peptide chemoattractant receptor is encoded by a 2 kilobase ...
Induces Differentiation and Apoptosis in Human Promyelocytic Leukemia HL-60 Cells. Lee Kyung-Tae, Sohn Il-Cheol, Kim Young-Kwan ...
The "selenite-induced cell death could be derived from necrosis rather than apoptosis, since selenite did not significantly ... "Se-Methylselenocysteine induces apoptosis through caspase activation in HL-60 cells". Carcinogenesis. 22 (4): 559-65. doi: ... It is an inhibitor of DMBA-induced mammary tumors and a "chemopreventive agent that blocks cell cycle progression and ... "Selenium Metabolism in Cancer Cells: The Combined Application of XAS and XFM Techniques to the Problem of Selenium Speciation ...
Méhes G, Pajor L (Jun 1995). "Nucleolin and fibrillarin expression in stimulated lymphocytes and differentiating HL-60 cells. A ... European Journal of Cell Biology. 75 (2): 174-83. doi:10.1016/s0171-9335(98)80059-9. PMID 9548374. Ai LS, Lin CH, Hsieh M, Li C ... The Journal of Cell Biology. 113 (4): 715-29. doi:10.1083/jcb.113.4.715. PMC 2288999. PMID 2026646. "Entrez Gene: FBL ... Experimental Cell Research. 257 (2): 272-80. doi:10.1006/excr.2000.4886. PMID 10837141. Pellizzoni L, Baccon J, Charroux B, ...
"Harmine showed cytotoxicity against HL60 and K562 cell lines. This could explain the cytotoxic effect of Peganum harmala on ... These islet sub-cells are normally very resistant to growth stimulation in the adult stage of a human's life, as the cell mass ... Harmine has been shown to promote differentiation of osteoblasts (bone-forming cells), and chondrocytes (cells in the cartilage ... including pancreatic α and β cells. The alteration of many other enzymes and genes that are implicated in cell proliferation ...
The main difference between these two MSPs is that the host cell for A. phagocytophilum is the granulocyte, while the host cell ... "Anaplasma phagocytophilum-induced gene expression in both human neutrophils and HL-60 cells". Genomics. 92 (3): 144-51. doi: ... It is also characterized by a low number of platelets, a low number of white blood cells, and elevated serum transaminase ... The bacteria infect white blood cells called neutrophils, causing changes in gene expression that prolong the life of these ...
The C5a receptor is expressed on: Granulocytes Monocytes Dendritic cells Hepatoma-derived cell line HepG2 Astrocytes Microglia ... "Expression cloning of a receptor for C5a anaphylatoxin on differentiated HL-60 cells". Biochemistry. 30 (12): 2993-9. doi: ... Beil WJ, Schulz M, Wefelmeyer U (July 2000). "Mast cell granule composition and tissue location--a close correlation". ... "CD88 antibodies specifically bind to C5aR on dermal CD117+ and CD14+ cells and react with a desmosomal antigen in human skin". ...
Lee JS; Lee JS; Roh HL; Kim CH; Jung JS; Suh KT (2006). "Alterations in the differentiation ability of mesenchymal stem cells ... Suh KT; Kim SW; Roh HL; Youn MS; Jung JS (2005). "Decreased osteogenic differentiation of mesenchymal stem cells in alcohol- ... The rapidity with which premature cell death can occur depends on the cell type and the degree and duration of the anoxia. ... In healthy bone these cells are constantly replaced by differentiation of bone marrow mesenchymal stem cells (MSC). However, in ...
Kagami S, Rizzo HL, Lee JJ, Koguchi Y, Blauvelt A (May 2010). "Circulating Th17, Th22, and Th1 cells are increased in psoriasis ... IL-22 takes effect on non-hematopoietic cells - mainly stromal and epithelial cells. Effects involve stimulation of cell ... Shen H, Goodall JC, Hill Gaston JS (June 2009). "Frequency and phenotype of peripheral blood Th17 cells in ankylosing ... IL-22 is produced by several populations of immune cells at a site of inflammation. Producers are αβ T cells classes Th1, Th22 ...
Polyphenolics in Their Glycoside and Aglycone Forms Induce Apoptosis of HL-60 Leukemia Cells". Journal of Agricultural and Food ...
Cell. Biol. 21 (18): 6189-97. doi:10.1128/MCB.21.18.6189-6197.2001. PMC 87336. PMID 11509662. Kohroki J, Fujita S, Itoh N, et ... 2001). "ATRA-regulated Asb-2 gene induced in differentiation of HL-60 leukemia cells". FEBS Lett. 505 (2): 223-8. doi:10.1016/ ... In myeloid leukemia cells, the expression of this encoded protein has been shown to induce growth inhibition and chromatin ... 2002). "ASB-2 inhibits growth and promotes commitment in myeloid leukemia cells". J. Biol. Chem. 277 (1): 218-24. doi:10.1074/ ...
Molecular Cell Research. 1450 (3): 364-73. doi:10.1016/S0167-4889(99)00056-7. PMID 10395947. Guan RJ, Ford HL, Fu Y, Li Y, Shaw ... "Enhanced expression of a novel protein in human cancer cells: a potential aid to cancer diagnosis". Cell Biology and Toxicology ... In mast cells, NDRG1 is upregulated during maturation and helps to rapid degranulation, which leads to enhanced exocytosis in ... Also was shown its role in T-cell clonal anergy downstream of Egr2, where NDRG1 is upregulated in the absence of costimulation ...
Cell. 7 (4): 585-95. doi:10.1016/j.devcel.2004.07.021. hdl:1721.1/83508. PMID 15469846. Human APBB1IP genome location and ... "The retinoic acid-responsive proline-rich protein is identified in promyeloleukemic HL-60 cells". J. Biol. Chem. 278 (51): ... Shattil SJ (2005). "Integrins and Src: dynamic duo of adhesion signaling". Trends Cell Biol. 15 (8): 399-403. doi:10.1016/j.tcb ... 2009). "RIAM regulates the cytoskeletal distribution and activation of PLC-gamma1 in T cells". Sci Signal. 2 (99): ra79. doi: ...
Luo B, Regier DS, Prescott SM, Topham MK (2005). "Diacylglycerol kinases". Cell. Signal. 16 (9): 983-9. doi:10.1016/j.cellsig. ... Batista EL, Warbington M, Badwey JA, Van Dyke TE (2005). "Differentiation of HL-60 cells to granulocytes involves regulation of ... Diacylglycerol kinase gamma has been implicated in cell cycle regulation and in the negative regulation of macrophage ... Topham MK (2006). "Signaling roles of diacylglycerol kinases". J. Cell. Biochem. 97 (3): 474-84. doi:10.1002/jcb.20704. PMID ...
In A549 cells, where cytotrienin A has been shown to be less effective, cyt A inhibits the expression of ICAM-1. This occurs ... The concentrations needed to induce this pathway were found to be the same required to induce apoptosis on HL-60 cells. Cyt A ... It was also shown that the metabolite has greater growth inhibitory activity on HL-60 cells at low concentrations and little ... Cytotrienin A induces apoptosis on HL-60 cells, as well as inhibiting translation in eukaryotes by inhibiting eukaryotic ...
... beta toxin forms cation-selective pores in cell membranes of 1.6-1.8 nm and results in swelling and lysis in HL60 cells. ... Heat-stable beta-toxin oligomers are shown to bind to cell membranes of human umbilical vein endothelial cells; endothelial ... Treatment of these cells with beta toxin induces an efflux of K+ and influxes of Ca2+, Cl− and Na+. ... The first 27 amino acids may encode a signal that allows beta toxin to cross the cell membrane, further evidenced by the ...
Kawasaki H, Taira K (2004). "MicroRNA-196 inhibits HOXB8 expression in myeloid differentiation of HL60 cells". Nucleic Acids ... and cell cycle in mice lacking miRNA-1-2". Cell. 129 (2): 303-17. doi:10.1016/j.cell.2007.03.030. PMID 17397913. Thum T, ... B-cell migration/adhesion, cell-cell interactions in immune niches and the production and class-switching of immunoglobulins. ... In malignant B cells miRNAs participate in pathways fundamental to B cell development like B-cell receptor (BCR) signalling, ...
Walsh CJ, Luer CA, Bodine AB, Smith CA, Cox HL, Noyes DR, Maura G (December 2006). "Elasmobranch immune cells as a source of ... Cancer stem cells may arise from transformation of adult stem cells or differentiated cells within a body. These cells persist ... and stem cells are the only cells that can transmit DNA from the zygote to cells late in life. Other cells, derived from stem ... The cancer stem cell hypothesis proposes that the different kinds of cells in a heterogeneous tumor arise from a single cell, ...
Asymmetric cell division has also been described in polyploid giant cancer cells and low eukaryotic cells and reported to occur ... "Retinoic acid induction of nuclear envelope-limited chromatin sheets in HL-60". Experimental Cell Research. 245 (1): 91-104. ... Spontaneous cell transformation: karyoplasts derived from multinucleated cells produce new cell growth in senescent human ... "Asymmetric cell division in polyploid giant cancer cells and low eukaryotic cells". BioMed Research International. 2014: 432652 ...
SH3 domain-binding site in SLP-76 required for T-cell receptor-mediated activation of PLC-gamma1 and NFAT". Mol. Cell. Biol. 21 ... Seo YJ, Jeon MH, Lee JH, Lee YJ, Youn HJ, Ko JH, Lee JH (Dec 2005). "Bis induces growth inhibition and differentiation of HL-60 ... linker for activation of T cells) required for recruitment and activation of signalling proteins in T cells". Biochem. J. 356 ( ... the ZAP-70 tyrosine kinase substrate that links T cell receptor to cellular activation". Cell. 92 (1): 83-92. doi:10.1016/S0092 ...
Atkins KB, Troen BR (July 1995). "Regulation of cathepsin D gene expression in HL-60 cells by retinoic acid and calcitriol". ... Conner GE, Udey JA (1990). "Expression and refolding of recombinant human fibroblast procathepsin D". DNA and Cell Biology. 9 ( ... Westley BR, May FE (May 1987). "Oestrogen regulates cathepsin D mRNA levels in oestrogen responsive human breast cancer cells ... Journal of Cell Science. 117 (Pt 21): 5155-64. doi:10.1242/jcs.01396. PMID 15456852. Jha, Sawan Kumar; Rauniyar, Khushbu; ...
Cells called phagocytes engulf pathogens and then use hydrogen peroxide to destroy them. The peroxide is toxic to both the cell ... McGraw-Hill Education. doi:10.1036/1097-8542.329200. Archived from the original on 30 November 2018. Retrieved 28 November 2018 ... It is also difficult to raise the level of oxygen around cancer cells within a tumour, as the blood supply tends to be poor, a ... In addition, when excess H2O2 accumulates in the cell, catalase converts it to H2O through this reaction: H 2 O 2 → CAT 1 2 O 2 ...
Cell. Biol. 22 (16): 5708-20. doi:10.1128/MCB.22.16.5708-5720.2002. PMC 133969. PMID 12138183. Strausberg RL, Feingold EA, ... 2006). "1,25-Dihydroxyvitamin D3 suppresses exportin expression in human promyelocytic leukemia HL-60 cells". Biomed. Res. 27 ( ... Mol Cell. 1 (3): 359-69. doi:10.1016/S1097-2765(00)80036-2. PMID 9660920. Arts GJ, Fornerod M, Mattaj IW (May 1998). " ...
... and is targeted to specific parts of the cell such as the Golgi where it may play a role in transport or proteins in cells. ... Kwon KB, Park EK, Ryu DG, Park BH (2003). "D4-GDI is cleaved by caspase-3 during daunorubicin-induced apoptosis in HL-60 cells ... Cell Res. 209 (2): 165-74. doi:10.1006/excr.1993.1298. PMID 8262133. Adra CN, Manor D, Ko JL, Zhu S, Horiuchi T, Van Aelst L, ... Cell. Biol. 14 (5): 3459-68. doi:10.1128/mcb.14.5.3459. PMC 358710. PMID 7513052. Adra CN, Kobayashi H, Rowley JD, Lim B (1995 ...
Cells. 10 (6): 733-9. doi:10.1007/s10059-000-0733-x. PMID 11211881. S2CID 10539699. Berleth ES, Henn AD, Gurtoo HL, et al. ( ... Conversely, the related protein SH3BGRL is reported to be downregulated in fibroblasts, lymphoid cells, and splenic tumor cells ... This protein is potentially involved in resistance of cells to the apoptosis-inducing effect of TNF-α. GRCh38: Ensembl release ... Seo J, Kim M, Kim J (2001). "Identification of novel genes differentially expressed in PMA-induced HL-60 cells using cDNA ...
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Their potential anticancer activity has been suggested by their induction of apoptosis in HL-60 cells. Shearinines D, E and G ...
In normal cells, this protein is localized to the cytoplasm; however, in cells undergoing apoptosis, it forms ball-like ... aggregates during apoptosis of human promyelocytic leukemia HL-60 cells". J. Biol. Chem. 274 (48): 33835-8. doi:10.1074/jbc. ... Cell. 10 (2): 417-26. doi:10.1016/S1097-2765(02)00599-3. PMID 12191486. Stehlik C, Fiorentino L, Dorfleutner A, Bruey JM, Ariza ... "Effects of methylation on expression of TMS1/ASC in human breast cancer cells". Oncogene. 22 (22): 3475-88. doi:10.1038/sj.onc. ...
Bannink N, Nout E, Wolvius EB, Hoeve HL, Joosten KF, Mathijssen IM (February 2010). "Obstructive sleep apnea in children with ... Certain cells in the brain respond specifically to an increase of CO2 in the blood.[4][24] The response involves vasodilatation ... Bristol RE, Lekovic GP, Rekate HL (December 2004). "The effects of craniosynostosis on the brain with respect to intracranial ... 60 (3): 146-50. doi:10.1002/(SICI)1096-9926(199909)60:3,146::AID-TERA10,3.0.CO;2-3. PMID 10471899.. ...
... a type 2 diabetic will have lost about half of their beta cells.[52] Fatty acids in the beta cells activate FOXO1, resulting in ... Fox, Caroline S.; Golden, Sherita Hill; Anderson, Cheryl; Bray, George A.; Burke, Lora E.; de Boer, Ian H.; Deedwania, Prakash ... Type 2 diabetes is due to insufficient insulin production from beta cells in the setting of insulin resistance.[13] Insulin ... In the early stages of insulin resistance, the mass of beta cells expands, increasing the output of insulin to compensate for ...
The] clave pattern has two opposing rhythm cells: the first cell consists of three strokes, or the rhythm cell, which is called ... Style: Maracatu 2:3; L.H.L.H.,LH.HL.H.. *Style: Samba-Reggae or Bossanova 3:2; O..O..O.,..O..O.. ... The second cell has two strokes and is called the two-side of the weak part of the clave. . . The different accent types in the ... Clave is the basic period, composed of two rhythmically opposed cells, one antecedent and the other consequent.[d][e] Clave was ...
Invasins, such as pneumolysin, an antiphagocytic capsule, various adhesins, and immunogenic cell wall components are all major ... McGraw Hill. ISBN 978-0-8385-8529-0.. .mw-parser-output cite.citation{font-style:inherit}.mw-parser-output .citation q{quotes ... and white blood cells to fill the alveoli. This condition is called pneumonia.[20] It is susceptible to clindamycin.[21] ... 60: 451-75. doi:10.1146/annurev.micro.60.080805.142139. PMID 16771651.. *^ Engelmoer DJ, Rozen DE (December 2011). "Competence ...
McGraw-Hill, Inc. pp. 589-592. ISBN 0-07-016190-9. .. *^ a b Barbalace, Kenneth. "Periodic Table of Elements Sorted by Melting ... The element is known to damage cell membranes of water animals, causing several negative influences on reproduction and on the ... The high radioactivity of lawrencium would make it highly toxic to living cells, causing radiation poisoning. The same is true ... The radioactivity of the actinides generally makes them highly toxic to living cells, causing radiation poisoning. ...
The TH2 lymphocytes interact with B cells and together they produce IgE. IgE circulates around and binds to receptors of cells ... McGraw-Hill Medical. pp. 364-375. ISBN 9780071481274. .. ... the Antigen-Presenting Cell causes a response in a TH2 ...
... epidermal hair cells (trichomes), cells in the stomatal complex; guard cells and subsidiary cells. The epidermal cells are the ... McGraw-Hill.. *. Hawthorne, William; Lawrence, Anna (2013). Plant Identification: Creating User-Friendly Field Guides for ... Cells that bring water and minerals from the roots into the leaf.. Phloem. Cells that usually move sap, with dissolved sucrose( ... Its cells contain many more chloroplasts than the spongy layer. Cylindrical cells, with the chloroplasts close to the walls of ...
... dendritic cells and other cells including liver cells, fibroblasts, and adrenal gland cells.[93] Viral replication triggers ... 2011). "Chapter 197". Harrison's Principles of Internal Medicine (18th ed.). McGraw-Hill. ISBN 978-0-07-174889-6. .. ... doi:10.1016/j.cell.2014.10.006. PMC 4243531. PMID 25417101.. *^ a b c d e f g h Kühl A, Pöhlmann S (September 2012). "How Ebola ... liver cells, and several types of immune cells such as macrophages, monocytes, and dendritic cells are the main targets of ...
... excess secretion from the acidophil cells) caused acromegaly, then an excess of basophil cells must be involved in another ... Storr, HL; Plowman PN; Carroll PV; François I; Krassas GE; Afshar F; Besser GM; Grossman AB; Savage MO. (2003). "Clinical and ... Given this conviction, and his knowledge of the three anterior pituitary cell types, Cushing hypothesized that if acidophil ... In a patient with Cushing's disease, the tumor cells will be stimulated to release corticotropin and elevated plasma ...
Because the cell acquiring a chloroplast already had mitochondria (and peroxisomes, and a cell membrane for secretion), the new ... Sun YJ, Forouhar F, Li Hm HM, Tu SL, Yeh YH, Kao S, Shr HL, Chou CC, Chen C, Hsiao CD (February 2002). "Crystal structure of ... and therefore topologically outside of the cell, because to reach the chloroplast from the cytosol, you have to cross the cell ... "The Plant Cell. 12 (1): 53-64. doi:10.1105/tpc.12.1.53. PMC 140214. PMID 10634907.. ...
McGraw-Hill Professional. p. 10. ISBN 0-07-145951-0. *^ a b Moskowitz, Sanford L. (2016). Advanced Materials Innovation: ... Low operating voltages compatible with batteries of only a few cells.. *Circuits with greater energy efficiency are usually ... Horowitz, Paul & Hill, Winfield (2015). The Art of Electronics (3 ed.). Cambridge University Press. ISBN 978-0521809269. .. CS1 ... From November 17, 1947, to December 23, 1947, John Bardeen and Walter Brattain at AT&T's Bell Labs in Murray Hill, New Jersey, ...
... and Th1 cells.[45] IL-1α stimulates increased skin cell activity and reproduction, which, in turn, fuels comedo development.[45 ... Kong YL, Tey HL (June 2013). "Treatment of acne vulgaris during pregnancy and lactation". Drugs (Review). 73 (8): 779-87. doi: ... and accumulation of skin cells in the hair follicle.[1] In healthy skin, the skin cells that have died come up to the surface ... the increased production of oily sebum causes the dead skin cells to stick together.[10] The accumulation of dead skin cell ...
Atkins HL, Freedman MS (January 2013). "Hematopoietic stem cell therapy for multiple sclerosis: top 10 lessons learned". ... who have lost their stem cells after birth. Other conditions[13] treated with stem cell transplants include sickle-cell disease ... Peripheral blood stem cells[26] are now the most common source of stem cells for HSCT. They are collected from the blood ... Sources and storage of cells[edit]. To limit the risks of transplanted stem cell rejection or of severe graft-versus-host ...
In medicine, this era brought innovations such as open-heart surgery and later stem cell therapy along with new medications and ... Kim JH 2010 ~Kim JH, Lau C, Cheuk K-K, Kan P, Hui HL, Griffiths SM. Brief report:predictors of heavy internet use and ... 7.Kim JH 2010 ~Kim JH, Lau C, Cheuk K-K, Kan P, Hui HL, Griffiths SM. Brief report: ... Some have described Karl Marx as a techno-optimist.[60]. Skepticism and critics. See also: Luddite, Neo-Luddism, Anarcho- ...
3.3 square kilometres (1.3 sq mi) of land in the Senri Hills near Osaka were put aside for its use. Tange and Uzo Nishiyama ... In his speech he used words such as "cell" and "metabolism" in relation to urban design. The Metabolist movement grew out of ... Richard Guy Wilson, The AIA Gold Medal (McGraw-Hill, 1984), p. 202. ... the hall he conceived was a fusion of Shinto shrine architecture and the plaza on Capitoline Hill in Rome. The design was not ...
Multiple tornadoes produced by the same storm cell are referred to as a "tornado family".[21] Several tornadoes are sometimes ... There are areas which people believe to be protected from tornadoes, whether by being in a city, near a major river, hill, or ... Tornadic storms do not contain more lightning than other storms and some tornadic cells never produce lightning at all. More ... 60 (4): 688-99. Bibcode:2008TellA..60..688R. doi:10.1111/j.1600-0870.2008.00331.x. hdl:2027.42/73164. Retrieved 2009-12-12.. ...
The generative cell in the pollen grain divides into two haploid sperm cells by mitosis leading to the development of the ... Enright, Neal J. and Robert S. Hill. 1990. Ecology of the southern conifers. Washington, DC: Smithsonian. 342pp. ... Then, the first tracheids of the transition zone are formed, where the radial size of cells and thickness of their cell walls ... At fertilization, one of the sperm cells unites its haploid nucleus with the haploid nucleus of an egg cell. The female cone ...
Alex Hill, jazz pianist. *John Henry "Doc" Holliday, famous gambler and gunslinger, suffered from tuberculosis until his death ... Friedrich Miescher Swiss biochemist, noted for discovery of nucleic acids in cell nucleus (1844-1895) ... Charles Bukowski (1920-1994), American author and poet, contracted TB in 1988; he recovered, losing 60 lbs. He died of leukemia ...
The spirochetes may also induce host cells to secrete quinolinic acid, which stimulates the NMDA receptor on nerve cells, which ... 2004). Sherris Medical Microbiology (4th ed.). McGraw Hill. pp. 434-37. ISBN 978-0-8385-8529-0. .. ... However, PCR tests are susceptible to false positive results, e.g. by detection of debris of dead Borrelia cells or specimen ... 2010). "Chapter 6, Structure, Function and Biogenesis of the Borrelia Cell Envelope". Borrelia: Molecular Biology, Host ...
"Francium". McGraw-Hill Encyclopedia of Science & Technology. 7. McGraw-Hill Professional. 2002. pp. 493-494. ISBN 978-0-07- ... Potassium is the major cation (positive ion) inside animal cells,[223] while sodium is the major cation outside animal cells.[ ... The balance between potassium and sodium is maintained by ion transporter proteins in the cell membrane.[231] The cell membrane ... McGraw-Hill. pp. 201-203. ISBN 978-0-8306-3015-8. .. *^ Winter, Mark. "Uses". Francium. The University of Sheffield. Archived ...
In 1975, association with "HL-A1,8" (Current name: HLA A1-B8) was confirmed by serological typing of cells from myasthenics.[11 ... Ludwig H, Polymenidis Z, Granditsch G, Wick G (November 1973). "[Association of HL-A1 and HL-A8 with childhood celiac disease ... HL-A8 the second refined B-serotype to be uncovered became HLA-B8. Because of the frequency of the haplotype, homozygotes are ... The "HL-A1,8 phenotype" was found to be associated with severe systemic lupus erythematosus (SLE) (renal and central nervous ...
Anaplastic cells have lost total control of their normal functions and many have deteriorated cell structures. Anaplastic cells ... Longo, Dan L (2012). "369 Seizures and Epilepsy". Harrison's principles of internal medicine (18th ed.). McGraw-Hill. p. 3258. ... Necrotic cells send the wrong chemical signals which prevent phagocytes from disposing of the dead cells, leading to a buildup ... Glial cells such as Schwann cells in the periphery or, within the cord itself, oligodendrocytes, wrap themselves around the ...
Richmond Hill, Ontario: Firefly Books Ltd. 2008. pp. 117-118. ISBN 978-1-55407-366-5. .. ... Cell. 25 (4): 326-328. doi:10.1016/j.devcel.2013.05.011. PMID 23725759.. ... waddling like an elephantine lizard up Holborne Hill. ... 60] Of the great host of dinosaurs dominating the Mesozoic, ...
The viruses infect, amongst others, monocytes, macrophages, and dendritic cells. They attach to the cell surfaces via specific ... 2004). Sherris Medical Microbiology (4th ed.). McGraw Hill. ISBN 978-0-8385-8529-0. .. ... After entering the host cell, the viral genome is replicated in the rough endoplasmic reticulum (ER) and in the so-called ... After transmission from a mosquito, the viruses replicate in the lymph nodes and infect dendritic cells in particular. From ...
Huk DJ, Hammond HL, Kegechika H, Lincoln J (February 2013). "Increased dietary intake of vitamin A promotes aortic valve ... Wu J, Zern MA (2000). "Hepatic stellate cells: a target for the treatment of liver fibrosis". Journal of Gastroenterology. 35 ( ... Vitamin K prevents hypoprothrombinemia in rats and can sometimes control the increase in plasma/cell ratios of vitamin A.[49] ... Levine PH, Delgado Y, Theise ND, West AB (February 2003). "Stellate-cell lipidosis in liver biopsy specimens. Recognition and ...
Hill S, Yau K, Whitwam J (1992). "MAOIs to RIMAs in anaesthesia--a literature review". Psychopharmacology. 106 Suppl: S43-5. ... in neuronal cells as well as in synaptic vesicles; extracellular levels also increase which results in increased monoamine ... 161 (3): 353-60. doi:10.1192/bjp.161.3.353. PMID 1393304.. *^ Versiani M, Amrein R, Montgomery SA (September 1997). "Social ... 44 (2): 57-60. PMID 8237297.. *^ Alevizos B, Hatzimanolis J, Markianos M, Stefanis CN (April 1993). "Clinical, endocrine and ...
BaP was shown to cause genetic damage in lung cells that was identical to the damage observed in the DNA of most malignant lung ... Ramesh A1, Inyang F, Lunstra DD, Niaz MS, Kopsombut P, Jones KM, Hood DB, Hills ER, Archibong AE.Alteration of fertility ... BaP has an effect on the number of white blood cells, inhibiting some of them from differentiating into macrophages, the body's ... This gene is a transcription factor that regulates the cell cycle and hence functions as a tumor suppressor. By inducing G ( ...
Molecular Neuropharmacology: A Foundation for Clinical Neuroscience (2nd ed.). New York: McGraw-Hill Medical. pp. 246, 248-259 ... There, CRH and vasopressin act synergistically to stimulate the secretion of stored ACTH from corticotrope cells. ACTH is ... in immune cells, such as monocytes and neutrophils [8][9][11][12] ... 7 (4): 257-60. doi:10.1080/10253890500044521. PMID 16019591.. *^ Buitelaar JK, Huizink AC, Mulder EJ, de Medina PG, Visser GH ( ...
2004). Sherris Medical Microbiology (4th ed.). McGraw Hill. ISBN 978-0-8385-8529-0. .. .mw-parser-output cite.citation{font- ... It is heat resistant and is capable of sequestering itself inside white blood cells, which may contribute to its persistence in ...
Klein HL (2008). "The consequences of Rad51 overexpression for normal and tumor cells". DNA Repair (Amst.). 7 (5): 686-93. doi: ... Esophageal squamous cell cancer. Over-expression. 47%. Immunohistochemistry. [24]. Renal cell carcinoma. Under-expression. 100% ... "Association of BRCA1 with Rad51 in mitotic and meiotic cells". Cell. 88 (2): 265-75. doi:10.1016/s0092-8674(00)81847-4. PMID ... Non-small-cell lung cancer. Over-expression. 29%. Immunohistochemistry. [22]. Soft tissue sarcoma. Over-expression. 95%. ...
Antiproliferative activity of various Uncaria tomentosa preparations on HL-60 promyelocytic leukemia cells.. Pilarski R1, ... potency of several cats claw preparations with different quantitative and qualitative alkaloid contents on HL-60 acute ... promyelocytic human cells by applying trypan blue exclusion and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide ...
Loss of TRF2 by radiation-induced apoptosis in HL60 cells.. Nakagami Y1, Ito M, Hara T, Inoue T. ... that gamma-rays induce the temporary accumulation and subsequent loss of TRF2 protein in the nuclei of irradiated HL60 cells. ...
Cell counting and MTT assay. To quantify the number of cells after each treatment, cells were counted at different incubation ... Cell culture. HL-60 cells were maintained in RPMI-1640 medium supplemented with 100 U/mL penicillin, 100 µg/mL streptomycin, 1 ... Flow cytometry analysis of cell-surface differentiation and antigen expression. HL-60 differentiation yields both monocytic and ... A, Cells were treated with various concentrations of TMP for the indicated times. The growth of HL-60 cells was significantly ...
A. W. Boyd and D. Metcalf, "Induction of differentiation in HL60 leukaemic cells: a cell cycle dependent all-or-none event," ... Antileukemic Potential of Momordica charantia Seed Extracts on Human Myeloid Leukemic HL60 Cells. Ramani Soundararajan,1,2 ... H. Tanaka, E. Abe, and C. Miyaura, "1α,25-Dihydroxycholecalciferol and a human myeloid leukaemia cell line (HL-60). The ... R. Levenson and D. Housman, "Memory of MEL cells to a previous exposure to inducer," Cell, vol. 17, no. 3, pp. 485-490, 1979. ...
on two human leukemic cancer cell lines (K562 and HL-60) and J774 as normal cells using alamarBlue (resazurin) assay. PI ... Sub-G1 peak in the flow cytometry histogram of the cells treated with CH,sub,2,/sub,Cl,sub,2,/sub, extract of ,i,A. turanica,/i ... The CH,sub,2,/sub,Cl,sub,2,/sub, extract of ,i,A. turanica,/i, showed the most antiproliferative effect on cancer cells among ... g/mL on K562 and HL-60 cells, respectively, whereas the normal cells were not affected significantly by this extract. ...
The results clearly suggest that fluoride causes cell death in HL-60 cells by causing the activation of caspase-3 which in turn ... Fluoride-induced cell cycle arrest, apoptosis, and DNA damage in TM3 mouse leydig cells. This study was designed to evaluate ... cells was investigated and the involvement of caspase-3 was also studied. Fluoride induced apoptosis in HL-60 cells in a dose- ... and DNA damage in TM3 mouse Leydig cells in vitro by the methods of flow cytometry (FCM) and single cell gel electrophoresis ( ...
... experiments were also carried out in the cultured human promyelocytic leukemia cell line HL-60 after differentiation with ... Chemotactic peptide activation of human neutrophils and HL-60 cells. Pertussis toxin reveals correlation between inositol ... Chemotactic peptide activation of human neutrophils and HL-60 cells. Pertussis toxin reveals correlation between inositol ... Pertussis toxin pretreatment of both cell types inhibited FMLP stimulated membrane depolarization, exocytosis, and superoxide ...
LA significantly inhibited cell proliferation of HL-60 cells and induced cell apoptosis through down regulating Bcl-2 and up ... The effect of LA on cell proliferation of HL-60 cancer cells were determined by MTT assay. The morphological effects of LA ... Flow cytometry was carried out to observe changes in cell-cycle distribution of the cells. The expression of Bcl-2 and Bax ... Flow cytometric analysis showed suppressed cell proliferation associated with cell cycle arrest in the G0/G1 phase. ...
... animal-origin free formulation for the transfection of DNA into eukaryotic cells with low cytotoxicity. ... The addition of antibiotics to media during transfection may result in cell death, and has not been tested for HL-60 cells. If ... The day of transfection, count the cells to determine culture density. Plate 1 x 105 cells per well in 0.5 ml of complete ... Use low-passage cells; make sure that cells are healthy and greater than 90% viable before transfection. ...
Exposure of HL-60 cells to sodium nitroprusside (SNP) strongly acti … ... or NO plus PMA-induced apoptotic cell death in HL-60 cells. ... Exposure of HL-60 cells to sodium nitroprusside (SNP) strongly ... Modulation of nitric oxide-induced apoptotic death of HL-60 cells by protein kinase C and protein kinase A through mitogen- ... In contrast, treatment of cells with phorbol 12-myristate 13-acetate (PMA) strongly activated not only JNK but also ERK, while ...
... in dimethylsulfoxide-differentiated HL-60 cells, as assessed by the fluorescence of quin-2. The increases in [Ca+2]i reached a ... Cell Differentiation / drug effects * Cell Line * Cytosol / drug effects * Cytosol / metabolism * Dimethyl Sulfoxide / ... The capacity of pretreatment of HL-60 cells with LTD4 to prevent subsequent responses of [Ca+2]i to LTC4 and LTD4, and the ... Thus, the increase in [Ca+2]i in HL-60 cells, which is coupled to an increase in adherence, appears to involve LTD4 receptor- ...
Inhibitors of coenzyme A-independent transacylase induce apoptosis in human HL-60 cells.. J D Winkler, T Eris, C M Sung, M ... Inhibitors of coenzyme A-independent transacylase induce apoptosis in human HL-60 cells.. J D Winkler, T Eris, C M Sung, M ... Inhibitors of coenzyme A-independent transacylase induce apoptosis in human HL-60 cells.. J D Winkler, T Eris, C M Sung, M ... ET-18-O-CH3 and other inhibitors of CoA-IT were found to inhibit cell proliferation and thymidine incorporation into the DNA, ...
a, Western blot analysis on whole cell lysates (Cell), cytosol (Cyt) and nuclei (Nu) after treatment of cells with 1 μm ATRA ... evaluation of cell viability (c), cell growth (d), and percentage of differentiated cells (e) during WT administration. C, ... Cell Culture and Differentiation.. HL-60 cells were cultured and differentiated as described previously (8) . Cell ... b, effect of ATRA on the number of fully differentiated cells after transfection of HL-60 with sense and antisense constructs. ...
In vitro inhibitory concentration required against human leukemic HL-60 cell line was determined after 48 hrs of drug exposure ...
Calyculin-A augmented the 1,25(OH)2D3-induced differentiation of the cells. Treatment of the cells with 1,25(OH)2D3 led to a ... The correlation between phenotypic and functional changes of HL-60 cells on the one hand and subcellular redistribution of PP1- ... 25-Dihydroxyvitamin D3-induced Monocytic Differentiation of HL-60 Cells. Serdar B. Omay, Hitoshi Ogasawara, Hideki Toyoda, ... 25-Dihydroxyvitamin D3-induced Monocytic Differentiation of HL-60 Cells. Serdar B. Omay, Hitoshi Ogasawara, Hideki Toyoda, ...
This chemical selectivity, together with the lack of apoptotic activity against rat Leydig cells, argues against a general cell ... and DNA fragmentation in three human cell lines, HL-60 promyelocytic, Jurkat T lymphoma, and Hut-78 s.c. lymphoma cells. ... Pyrrolo-1,5-benzoxazepines Induce Apoptosis in HL-60, Jurkat, and Hut-78 Cells: A New Class of Apoptotic Agents. Daniela M. ... Pyrrolo-1,5-benzoxazepines Induce Apoptosis in HL-60, Jurkat, and Hut-78 Cells: A New Class of Apoptotic Agents. Daniela M. ...
Tested for inhibition of cell adhesion of HL-60 containing SLeX to purified recombinant human E-selectin. ...
Arecaceae) induces apoptosis of human colon cancer Ht-29 cells. Pak. J. Biol. Sci., 14: 578-583.. CrossRef , Direct Link , ... Molecular Mechanisms of Apoptosis Induced by Scytosiphon gracilis Kogame in HL-60 Cells ... Apoptosis via reactive oxygen species generation and caspase 3 activation in mouse P19 embryonal carcinoma cells. Int. J. ...
Isolation of variant HL60 cells. HECA-452−/low variants of HL60 were isolated by cloning at limiting dilution a parental HL60 ... The mean number of HL60 cells bound per COS cell was determined by counting the number bound to ∼100 COS cells in multiple ... A variant of HL60 cells lacks significant reactivity with HECA-452, but binds well to E- and P-selectin. The myeloid cell line ... Adhesion of HL60 or HL60var to transfected COS cells was conducted at 4°C with constant rocking. Each plate of COS cells was ...
Drug‑resistance in these cells may be due to surviving chemoresistant LSCs in the HL60 population, which have been subjected ... Unlike other recognized chemoresistant leukemia cell lines, HL60/RS cells were also strongly cross‑resistant to arsenic ... The drug‑resistant HL60/RS cells exhibited 85.68‑fold resistance to doxorubicin and were cross‑resistant to other ... The cells over‑expressed the transporters P‑glycoprotein, multidrug‑resistance‑related protein 1 and breast‑cancer‑resistance ...
... induces the proliferation and maturation of immature myeloid progenitor cells and primes mature cell function in phagocytes. To ... GM-CSF mediated activation of STAT 5 was markedly increased (15-20-fold) after differentiation of HL-60 cells but the kinetics ... These data show that in the HL-60 cell model, differentiation modulates the activation of signalling molecules downstream of ... Differentiation-linked changes in granulocyte-macrophage colony-stimulating factor receptor mediated signalling in the HL-60 ...
... cells and prostate cancer (PC-3) cells. Cytotoxicity of D. psilurus extract was tested in HL-60 and PC-3 cells using 3-(4, 5- ... modification in the DNA distribution and enhance of G2/M phase cell cycle. The extract induces apoptosis of HL-60 cells ... psilurus have significant in vitro cytotoxic activity in HL-60 cells and PC-3 cells with IC50 value of 12 ±1.54 μg/ml and 18 ± ... psilurus extract induces apoptosis on HL-60 cells by the generation of reactive oxygen species (ROS) along with concurrent loss ...
METHODS: MTT cell viability assay was used to verify the sensitization of DNR by quercetin in K562/ADM and HL 60/ADM cells,and ... and relevant sensitive cell lines, K562/S and HL 60/S,before and after quercetin exposion. RESULTS: Compared with K562/S and HL ... in multidrug resistant leukemia cell lines,K562/ADM and HL 60/ADM,and reversing their MDR. ... 20-40 μmol/L of quercetin in vitro remarkably enhanced the sensitivity of K562/ADM and HL 60/ADM cells to DNR,restore the ...
Me2SO the cells differentiate within 5-7 days into cells resembling neutrophils in both structure and function. Treated cells ... The Ins(1,4,5)P3 receptor was examined in human promyelocytic leukaemic cells (HL-60) and in HL-60 cells differentiated towards ... Concomitant with HL-60 cell differentiation, the maximal [3H]Ins(1,4,5)P3 binding in membranes increases 3-4-fold, with no ... The results suggest that there is an absolute increase in the level of the InsP3 receptor during HL-60 cell differentiation and ...
Culturable cells aid in assay standardization and likely reduce donor-to-donor variability. This standardized assay, in ... The results of an opsonophagocytic assay with HL-60 cells correlated highly with those of an assay with PBLs as effector cells ... We developed and standardized an opsonophagocytic assay using HL-60 cells (human promyelocytic leukemia cells). Fifty-five ... when HL-60 cells were used as effector cells and 0.56, 0.47, 0.61, 0.90, 0.71, 0.31, and 0.62, respectively, when PBLs were ...
In some cases, cells were pretreated with CC (Sigma Chemical Co.) to inhibit PKC activity. Phagocytosis.. 5 x 105 cells/ml HL- ... was greatly enhanced in PMA-induced cells: it was 5 ng/2.5 x 105 cells/ml (. 100-fold higher than in undifferentiated HL-60 ... In B, Lane 1 represents untreated cells. HL-60 cells were induced with 10 nM PMA or 100 nM VitD3 (Lanes 2 and 3) for 48 h. HL- ... This cell line can be induced to differentiate into monocytic cells by incubation with VitD3 (3) or into macrophage-like cells ...
Laboratory of Immune Cells Signaling, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, ... we examined differentiation in the model human myeloid cell line, HL-60, treated with phorbol 12-myristate 13-acetate (PMA) or ... Cell Growth & Differentiation Vol. 13, 27-38, January 2002. © 2002 American Association for Cancer Research Expression of Toll- ... Cell Growth & Differentiation. Copyright © 2002 by the American Association of Cancer Research. ...
In Pam3CysSer(Lys)4-differentiated HL-60 cells, the responsiveness to the [Ca2+]i-increasing agonists, N-formyl-L-methionyl-L- ... J. 267, 795-802). We studied the effects of lipopeptides on differentiation of HL-60 leukemic cells. Pam3CysSer(Lys)4 enhanced ... Pam3CysSer(Lys)4 did not inhibit proliferation of HL-60 cells but decreased transferrin receptor expression and increased C3bi ... Our results suggest that (a) Pam3CysSer(Lys)4 induces incomplete functional differentiation of HL-60 cells through a mechanism ...
In dHL60 cells treated with fMLP to cause cell spreading and polarization, Pyk2 was concentrated at the leading edge of ... Western blot analysis demonstrated that hematopoietic Pyk2 was predominantly expressed after HL60 cell differentiation. Pyk2 ... Adhesion of dHL60 cells to plated fibrinogen was measured by residual myeloperoxidase activity. dHL60 cell migration was ... Similarly, silencing of Pyk2 expression by siRNA in dHL60 cells also attenuated dHL60 cell migration caused by fMLP. Phospho- ...
  • It has been reported that carotenoids from saffron were effective in inhibiting the proliferation of HL-60 cells [ 5 ]. (hindawi.com)
  • LA significantly inhibited cell proliferation of HL-60 cells and induced cell apoptosis through down regulating Bcl-2 and up regulating Bax expression. (aacrjournals.org)
  • Pam3CysSer(Lys)4 did not inhibit proliferation of HL-60 cells but decreased transferrin receptor expression and increased C3bi receptor expression. (uni-regensburg.de)
  • The proliferation of HL-60 cells was significantly inhibited in dose-and time-dependent manners after treating with quercetin (r = 0.77) and kaempferol (r = 0.76) respectively, and the cytotoxicity of quercetin was superior to that of kaempferol. (greenmedinfo.com)
  • Proliferation of HL-60 cells occurs through the transferrin and insulin receptors, which are expressed on cell surface. (wikipedia.org)
  • WS-LMF also inhibited proliferation of HL-60 cells in a dose-dependent manner and was cytotoxic at higher concentration (1000μg/mL). (elsevier.com)
  • Each fraction inhibited proliferation of HL-60 cells at a different UV spectra, suggesting that multiple anti-proliferation factors toward HL-60 cells are present in WS-LMF. (elsevier.com)
  • 2 Ginsenoside Rh2 potently inhibited the proliferation of HL-60 cells in both a dose- and time-dependent manner with an $IC_{50}$ , $20{\mu}M$ . 3 DNA flow-cytometry indicated that ginsenoside Rh2 markedly induced a $G_1$ phase arrest of HL-60 cells. (koreascience.or.kr)
  • In vitro studies have shown that micromolar concentrations of As 2 O 3 can induce apoptosis of leukemia cells, while at lower concentrations (0.1-0.5 µM) As 2 O 3 induces cell differentiation (3,4). (scielo.br)
  • Inhibitors of coenzyme A-independent transacylase induce apoptosis in human HL-60 cells. (aspetjournals.org)
  • ET-18-O-CH3 and other inhibitors of CoA-IT were found to inhibit cell proliferation and thymidine incorporation into the DNA, as well as to induce apoptosis in human HL-60 monocytic leukemia cells. (aspetjournals.org)
  • Furthermore the apoptosis-inducing effect of quercetin is stronger than that of kaempferol, both of which induce apoptosis of HL-60 cells through depressing cell growth, arresting cell cycle and inhibiting expression of survivin. (greenmedinfo.com)
  • The results suggest that 50 - 500 µM resveratrol did not induce apoptosis in calcitriol-treated cells. (viurrspace.ca)
  • In this report, baicalein was found to induce apoptosis in HL60 human promyelocytic leukemia cell line. (koreascience.or.kr)
  • Although it has been reported that baicalein can induce apoptosis through many caspase pathways, the present study indicates that caspase 2 not caspase 9 pathway may be the important step in apoptosis on HL60 cell line. (koreascience.or.kr)
  • These growth inhibitory effects correlated with externalization of phosphatidylserine and loss of mitochondrial membrane potential, suggesting that these isomeric steroids induce apoptosis in leukemia cells. (aacrjournals.org)
  • It was shown that the apoptotic cleavage followed after G1 blockade of the cell cycle. (europeana.eu)
  • These results suggest that resveratrol induced apoptotic cell death of HL-60 cells depends on the autophagy activated through both the LKB1-AMPK and PI3K/AKT-regulated mTOR signaling pathways. (biomedcentral.com)
  • Furthermore, we demonstrate that RSV-induced HL-60 cell death involves autophagy-dependent apoptotic cell death via both the LKB1-AMPK and PI3K/AKT-regulated mTOR signaling pathways. (biomedcentral.com)
  • The aim of this study is to investigate the cytotoxic and apoptotic potential of methanol extract of D. psilurus in human promyelocytic leukemia (HL-60) cells and prostate cancer (PC-3) cells. (biomedcentral.com)
  • The extract induces apoptosis of HL-60 cells associated with ROS production, loss of mitochondrial membrane potential and apoptotic DNA fragmentation. (biomedcentral.com)
  • The results of apoptotic related protein indicated that the induction of HL-60 cell death was caused by the induction of apoptosis through upregulation of Bax and downregulation of Bcl-2. (go.jp)
  • Western blot analysis was used to study the apoptotic mechanism of HL-60 cells. (greenmedinfo.com)
  • Ultraviolet B (UVB) radiation acts as a strong apoptotic trigger in many cell types, in tumor and normal cells. (mdpi.com)
  • In this review, we investigated UVB apoptotic action in various cell models by using ultrastructural, molecular and cytofluorimetric techniques. (mdpi.com)
  • Myeloid leukemia HL-60, T-lymphoblastoid Molt-4 and myelomonocytic U937 human cells, generally affected by apoptotic stimuli, were studied. (mdpi.com)
  • Cell apoptosis was assessed by Annexin V-FITC/PI staining: all three cancer cells treated with karanjin exhibited significantly increased apoptotic rates, especially in the percentage of late apoptosis cells. (uga.edu)
  • Moreover, a series of biochemical analyses determined that M-IN caused apoptotic bodies, DNA fragmentation, and enhanced PARP and pro-caspase 3 degradation in HL-60 cells. (elsevier.com)
  • And then, we detected the effect of cell apoptosis and proliferation, cell cycle distribution, changes of apoptotic proteins. (medsci.org)
  • The phagocytosis of apoptotic cells within an organism is a critical terminal physiological process in programmed cell death. (rupress.org)
  • We show that the class B scavenger receptor CD36 plays an essential role in macrophage clearance of apoptotic cells in vivo. (rupress.org)
  • Further, macrophage recognition of apoptotic cells via CD36 is shown to occur via interactions with membrane-associated oxidized PS (oxPS) and, to a lesser extent, oxidized phosphatidylcholine, but not nonoxidized PS molecular species. (rupress.org)
  • Collectively, these results identify oxPS-CD36 interactions on macrophages as potential participants in a broad range of physiologic processes where macrophage-mediated engulfment of apoptotic cells is involved. (rupress.org)
  • The terminal phase of programmed cell death, removal of apoptotic cells, is a critical homeostatic function involved in an array of diverse cellular processes ranging from embryonic development and tissue remodeling to resolution of inflammation ( 1 , 2 ). (rupress.org)
  • Controlled phagocytic engulfment of apoptotic cells suppresses inflammation by limiting cytolysis and necrosis ( 2 - 4 ). (rupress.org)
  • Multiple receptors are implicated in macrophage clearance of apoptotic cells including CD36, a prototypic member of the class B scavenger receptor family ( 5 - 11 ). (rupress.org)
  • In vitro studies suggest a role for the CD36 scavenger receptor in the recognition of apoptotic cells because both endogenous (e.g., macrophages and dendritic cells) and ectopic (e.g., melanoma cells and fibroblasts) expression of CD36 on the surface of cells confers phagocytic activity for engulfment of apoptotic cells ( 8 , 12 , 13 ). (rupress.org)
  • Despite the many demonstrations of CD36 recognition of apoptotic cells using in vitro model systems, direct demonstration of CD36 involvement in apoptotic cell clearance in vivo is lacking. (rupress.org)
  • The mechanisms through which CD36 recognizes apoptotic cells have not been clearly defined. (rupress.org)
  • 0.05) the expression and translocation of apoptotic molecules and decreasing the mitochondrial membrane potential in leukemia cells. (biomedcentral.com)
  • This apoptotic signaling is modulated via oxidative stress, DNA damage, and change in mitochondrial membrane potential, translocation and upregulation of apoptotic proteins leading programmed cell death. (biomedcentral.com)
  • Antiproliferative activity of various Uncaria tomentosa preparations on HL-60 promyelocytic leukemia cells. (nih.gov)
  • In this study, we focused on the effect of GLA induction on apoptosis, the mitochondria-mediated death pathway and the accumulation of reactive oxygen species (ROS) in human leukemia cells (HL-60). (nih.gov)
  • Increased responsiveness to TLR2 and TLR4 ligands during dimethylsulfoxide-induced neutrophil-like differentiation of HL-60 myeloid leukemia cells," Leukemia Research , vol. 31, no. 12, pp. 1721-1728, 2007. (hindawi.com)
  • Histamine H2 receptor desensitization in HL-60 human promyelocytic leukemia cells. (aspetjournals.org)
  • SKP2 and P27 kip1 affect the proceeding and prognosis of leukemia through regulating the proliferation, apoptosis and differentiation of leukemia cells. (scialert.net)
  • Down-regulating the SKP2 expression and arresting cells in the G 0 /G 1 phase improve drug sensitivity of leukemia cells with down-regulated MRP expression. (scialert.net)
  • We developed and standardized an opsonophagocytic assay using HL-60 cells (human promyelocytic leukemia cells). (asm.org)
  • In the present study, we examined the enhanced effect of ERW in the apoptosis of leukemia cells (HL-60) induced by glutathione (GSH). (go.jp)
  • The control of phospholipid methylation by phorbol diesters in differentiating human myeloid HL-60 leukemia cells. (semanticscholar.org)
  • Treatment of human HL-60 promyelocytic leukemia cells with phorbol-12-myristate-13-acetate (PMA), a tumor promoter and inducer of differentiation, stimulated the incorporation of label from L-[methyl-3H]methionine into the cellular phospholipids. (semanticscholar.org)
  • Membrane lipid dynamics in human promyelocytic leukemia cells sensitive and resistant to 12-O-tetradecanoylphorbol-13-acetate induction of differentiation. (semanticscholar.org)
  • Double minute chromatin bodies and other chromosome alterations in human myeloid HL-60 leukemia cells susceptible or resistant to induction of differentiation by phorbol-12-myristate-13-acetate. (semanticscholar.org)
  • In order to get insight on the potential mechanism of action, we compared the protein expression profile of HL-60 human leukemia cells after treatment with the free and peptide conjugated daunomycin. (uni-konstanz.de)
  • Curcumin inhibits leukemia cells in mice. (greenmedinfo.com)
  • WS-LMF was found to inhibit proliferation of human HL-60 leukemia cells and mouse B16 melanoma cells. (elsevier.com)
  • Schwartsmann, G, Pinedo, HM & Leyva, A 1987, ' Resistance of HL 60 promyelocytic leukemia cells to induction of differentiation and its reversal by combination treatment ', European Journal of Cancer and Clinical Oncology , vol. 23, no. 6, pp. 739-743. (vumc.nl)
  • Recently published studies from our laboratory have demonstrated that ATO pharmacology as an anti-leukemic drug is associated with cytotoxic and genotoxic effects in leukemia cells. (biomedcentral.com)
  • Human promyelocytic leukemia cells differentiate into monocytes or granulocytes when treated with 1,25-dihydroxyvitamin D3 [1,25-(OH) 2 D 3 ] or all-trans retinoic acid (ATRA). (elsevier.com)
  • GM-CSF stimulated MAP kinase activation in both the undifferentiated and differentiated HL-60 cells. (ovid.com)
  • Growth of Ehrlichia from both equine and human sources was enhanced by addition of retinoic acid, which causes granulocytic differentiation of the HL-60 cells. (asm.org)
  • Phosphoinositide 3-kinase (PI 3-K) activity increases in HL-60 cells that are induced to granulocytic differentiation by all- trans -retinoic acid. (aacrjournals.org)
  • Cell proliferation and CD11b expression are controlled independently during HL60 cell differentiation initiated by 1,25 alpha-dihydroxyvitamin D(3) or all-trans-retinoic acid. (biomedsearch.com)
  • Here we show that similar maturation divisions occur during neutrophil differentiation induced by all-trans-retinoic acid (ATRA), but without shortening of the cell cycle. (biomedsearch.com)
  • To investigate whether the biochemical events following the binding of GM-CSF to its receptor are differentiation dependent we analysed GM-CSF mediated activation of the JAK 2-STAT 5 and MAP kinase pathways in undifferentiated HL-60 cells and HL-60 cells induced to differentiate with dimethyl sulphoxide (DMSO) or retinoic acid (RA). (ovid.com)
  • The Ins(1,4,5)P3 receptor was examined in human promyelocytic leukaemic cells (HL-60) and in HL-60 cells differentiated towards granulocytes with either retinoic acid (RA) or dimethyl sulphoxide (Me2SO). (biochemj.org)
  • The retinoid signal is mediated in target cells through retinoic acid receptors (RAR) and retinoid X receptors (RXR), both of which are members of the nuclear receptor superfamily. (mdpi.com)
  • Combination of those fractions with retinoic acid had significant synergistic effects on the differentiation of cells (56.8 ± 3.7% and 67.4 ± 4.2%, p≤0.01). (waocp.org)
  • The activity of PAD4 has been examined in HL-60 cells that, following treatment with all- trans retinoic acid (ATRA), acquire properties of mature neutrophils. (jimmunol.org)
  • However, when HL-60 cells are induced to differentiate to granulocytes by treatment with retinoic acid (RA), they express CD38 and accumulate cADPR. (unige.it)
  • trans retinoic acid (ATRA)-mediated differentiation of human HL-60 cells, and identified PCGF2 as a gene down-regulated by ATRA in a time-dependent manner. (elsevier.com)
  • Using the HL60 multipotent promyelocytic leukemia cell line, we performed experiments that ultimately led to two different cell fate attractors by two treatments of varying dosage and duration of the differentiation agent all-trans-retinoic acid (ATRA). (biomedcentral.com)
  • from human and equine sources in HL-60 cells induced to differentiate into functional granulocytes. (asm.org)
  • When 1 alpha,25-dihydroxyvitamin D(3) (D(3)) induces HL60 cells to differentiate to monocytes, a burst of approximately three shortened cell cycles ("maturation divisions") precedes exit from cell cycle and completion of maturation. (biomedsearch.com)
  • When HL-60 cells are incubated with 1 microM-RA or with 1.25% Me2SO the cells differentiate within 5-7 days into cells resembling neutrophils in both structure and function. (biochemj.org)
  • HL-60 cells were induced to differentiate into neutrophil-like cells (dHL60) by incubation in medium containing 1.25% DMSO for up to 4 days. (biomedcentral.com)
  • When HL-60 cells are induced to differentiate by dimethyl sulfoxide along a granulocytic pathway there is a fivefold decrease in the total number of transferrin receptors within 3 days, as compared to untreated cells. (elsevier.com)
  • Other compounds like 1,25-dihydroxyvitamin D3, 12-O-tetradecanoylphorbol-13-acetate (TPA) and GM-CSF can induce HL-60 to differentiate to monocytic, macrophage-like and eosinophil phenotypes, respectively. (wikipedia.org)
  • Eosinophil granule specific staining with Luxol-fast-blue was used to determine if HL-60 cells could differentiate into the eosinophilic lineage. (elsevier.com)
  • These data suggest that GLA induces apoptosis in HL-60 cells through ROS-dependent mitochondrial dysfunction pathway. (nih.gov)
  • Cell proliferation was determined by using MTT assay. (hindawi.com)
  • Several studies have shown that TMP has various biological activities, such as antioxidant activity (7), the ability to modulate nitric oxide production (8), and cytotoxicity against various tumor cells (9). (scielo.br)
  • Lipofectamine® LTX Reagent is a proprietary, animal-origin free formulation for the transfection of DNA into eukaryotic cells with low cytotoxicity . (thermofisher.com)
  • However, there has not been any comparative study with hypericin and extract obtained from Hypericium perforatum L. In this study, it has been aimed to investigate the potential cytotoxic role of the extract obtained from Hypericium perforatum grown in Ege region on leukemic cell line, to compare the cytotoxic effects of both extract and hypericin in HL-60 cells, and to clarify the underlying mechanism(s) of this cytotoxicity. (tjh.com.tr)
  • Hypericin was found to have cytotoxicity in HL-60 cells in time and dose dependent manner between the doses of 1nM to 100 μM with IC50 dose of 0.5 μM. (tjh.com.tr)
  • These results indicate that during acute IM, suppressor T cells capable of inhibiting B cell activation in the absence of cytotoxicity can be identified, and may play a key role in the control of EBV infection. (jci.org)
  • On the other hand, the results showed that M-IN with an IC 50 value maintained at 36.9 μg/ml for 12 h exhibited stronger cytotoxicity than ethyl ester, γ-lactone derivatives of indomethacin, and indomethacin in promyelocytic leukemia HL-60 cells. (elsevier.com)
  • These above results indicate that the photosynthesized product, M-IN, had stronger anti-inflammatory effects in LPS-stimulated RAW 264.7 cells and cytotoxicity effects in HL-60 cells than the parent drug, indomethacin. (elsevier.com)
  • Flow cytometry was used to analyze cell cycle distribution. (scielo.br)
  • HL-60 cells were treated by crocin, and cell proliferation, apoptosis, and cell cycle profiles were examined by MTT assay, AO/EB staining, and flow cytometry, respectively. (hindawi.com)
  • Flow cytometry was carried out to observe changes in cell-cycle distribution of the cells. (aacrjournals.org)
  • The cell cycle was analyzed by flow cytometry. (scialert.net)
  • Using rhodamine 123 to stain mitochondria of the human cell line HL-60, we have followed their increase over the cell cycle by flow cytometry. (rupress.org)
  • The apoptosis of HL-60 cells in the presence/absence of MSCs and/or Daunorubicin were determined by flow cytometry with Annexin V/PI double staining. (bvsalud.org)
  • [email protected]#The flow cytometry showed that there was no significant change in apoptosis of HL-60 cells co-cultured with MSC derived from healthy donors or AML patients. (bvsalud.org)
  • The effect of quercetin and kaempferol on cell cycle in HL-60 cells was detected by flow cytometry. (greenmedinfo.com)
  • The induction of cell cycle arrest was observed via a PI (propidium iodide)/RNase Staining Buffer detection kit and analyzed by flow cytometry: karanjin could dose-dependently induce cell cycle arrest at G2/M phase in the three cell lines. (uga.edu)
  • Together, MTT and flow cytometry technology showed that cell growth was significantly inhibited. (medsci.org)
  • Flow cytometry was used to evaluate CD11b in the differentiated cells, and the NBT assay was used to evaluate the functionality of the differentiated HL-60 cells. (ac.ir)
  • a Annexin V-FITC/PI staining of A549 cells exposed to karanjin as analyzed by flow cytometry. (biomedcentral.com)
  • Incubating HL-60 cells in the presence of the specific H2 agonist dimaprit resulted in 30-fold increases in cAMP levels (EC50 = 5.7 X 10(-6) M) and morphological changes suggestive of cell maturation along the granulocyte pathway. (aspetjournals.org)
  • However, PI 3-K is activated also by factors that trigger cellular differentiation, such as nerve growth factor in PC12 cells (4) and DMSO in murine erythroleukemia (MEL) cells (5) , suggesting that PI 3-K and its lipid products may also play a role in cell maturation. (aacrjournals.org)
  • Both ATRA and D(3) initiate these maturation divisions as cells pass through a "window of sensitivity" during early G1. (biomedsearch.com)
  • Cells treated with D(3) or ATRA start to express CD11b after 9--14 h, before completing the first maturation division. (biomedsearch.com)
  • Differentiating agents must therefore regulate the proliferation and the maturation of differentiating myeloid cells by mechanisms that are at least partly independent. (biomedsearch.com)
  • Granulocyte-macrophage colony-stimulating factor (GM-CSF) induces the proliferation and maturation of immature myeloid progenitor cells and primes mature cell function in phagocytes. (ovid.com)
  • The T cell product interleukin 5 (IL-5) has been shown to be a key factor in the development and the maturation of the eosinophilic cell lineage. (rupress.org)
  • This suggests that they may interfere with normal cell maturation. (cdc.gov)
  • 2. Le Cabec V, Calafat J, Borregaard N. Sorting of the Specific Granule Protein, NGAL, During Granulocytic Maturation of HL-60 Cells. (ac.ir)
  • Apoptosis was induced by treatment of HL-60 cells with C2-ceramide. (europeana.eu)
  • Treatment of HL-60 cells with cyclin D3 antisense-DNA prior to phorbol myristate acetate addition abolished the growth inhibition seen in the control and sense-DNA groups. (elsevier.com)
  • We investigated whether treatment of HL-60 cells with Me(2)SO involves any changes in the cADPR/intracellular calcium ([Ca(2+)](i)) signaling system and, specifically, whether Me(2)SO affects those nucleoside transporters (NT) (both equilibrative (ENT) and concentrative (CNT)) that mediate influx of extracellular cADPR. (unige.it)
  • Treatment of HL-60 cells with zero to 100μg/ml of a methanol extract of ginseng for 72 h induced a small increase in cell differentiation. (elsevier.com)
  • The peptidic caspase-3 inhibitors DEVD-CHO (NH 2 -Asp-Glu-Val-Asp-CHO, 2µM), increased the viability of HL-60 cells, previously treated with LA. The results indicated that LA induced efficient cell apoptosis by activating the caspase-3 pathway and through down and up regulation of Bcl-2 and Bax expression respectively. (aacrjournals.org)
  • In our experience, regardless of the length of the nanotube, single-walled carbon nanotubes decrease while multi-walled counterparts do not affect the viability of HL-60 cells as detected by MTT tests. (nanopaprika.eu)
  • Western blot analyses and immunohistochemistry showed that gamma-rays induce the temporary accumulation and subsequent loss of TRF2 protein in the nuclei of irradiated HL60 cells. (nih.gov)
  • N2 - In this study we compare methods for large-scale microarray analysis of protein and RNA level changes in HL-60 cells, responding to differentiation stimuli. (muni.cz)
  • Western blotting analysis indicated that EG-mediated HL-60 apoptosis mainly occurred through the mitochondrial pathway, as shown by the release of cytochrome c , apoptosis-inducing factor (AIF), and endonuclease G (Endo G), as well as the upregulation of Bcl-2-associated X protein (Bax). (mdpi.com)
  • The only difference observed here between HL-60 cells and neutrophils concerned the differential effects of a protein kinase C inhibitor.In summary, the results presented here show that differentiated HL-60 cells, stimulated with chemotactic peptide, are a valid model system to study molecular mechanisms of neutrophil emigration. (sigmaaldrich.com)
  • Here, we demonstrate that PI 3-K increases after ATRA treatment and that specific inhibition of PI 3-K activity and down-modulation of the protein prevent the differentiation of this cell line. (aacrjournals.org)
  • To elucidate the roles of protein phosphatases type 1 (PP1) and type 2A (PP2A) in 1,25-dihydroxy-cholecalciferol [1,25(OH) 2 D 3 ]-induced differentiation of HL-60 cells into monocytes, we examined the enzyme activity and the protein and gene expressions of PP1 and PP2A in these cells. (aacrjournals.org)
  • Treatment of the cells with 1,25(OH) 2 D 3 led to a decrease in PP1-like activity in the cytosol fraction, with a concomitant increase in the membrane and nuclear PP1-like activity, as determined when protein phosphatase activity was assayed using myosin light chain as substrate in the presence of 5 n m okadaic acid. (aacrjournals.org)
  • The cells over‑expressed the transporters P‑glycoprotein, multidrug‑resistance‑related protein 1 and breast‑cancer‑resistance protein, encoded by the adenosine triphosphate‑binding cassette (ABC)B1, ABCC1 and ABCG2 genes, respectively. (spandidos-publications.com)
  • The expression of SKP2 in HL-60/A cells nucleofected by SKP2 siRNA was down-regulated whereas the protein level of P27 kip1 was up-regulated. (scialert.net)
  • Compared with the MRP expression level in the control group (nucleofected by control siRNA), the mRNA and protein expression levels of MRP in HL-60/A cells nucleofected by SKP2 siRNA were lower, and the latter cells were more sensitive to adriamycin, daunorubicin, and arabinosylcytosine. (scialert.net)
  • Electrotransfer studies indicate that Ins(1,4,[32P]5)P3 binds specifically to a 260 kDa protein of HL-60 cell membranes. (biochemj.org)
  • This Ins(1,4,5)P3-binding protein selectively binds Ca(2+)-mobilizing inositol phosphates and other inositol phosphates which also bind to the purified InsP3 receptor, suggesting that the Ins(1,4,5)P3-binding protein of HL-60 cell membranes is the InsP3 receptor. (biochemj.org)
  • The results suggest that there is an absolute increase in the level of the InsP3 receptor during HL-60 cell differentiation and that the expression of this signal-transducing protein may be specifically regulated by differentiation factors. (biochemj.org)
  • Protein-pattern changes and morphological effects due to methionine starvation or treatment with 5-azacytidine of the phorbol-ester-sensitive cell lines HL-60, CCL-119, and U-937. (semanticscholar.org)
  • TAT-Pyk2-CT, a dominant negative fusion protein in which the TAT protein transduction domain was fused to the c-terminal Pyk2, attenuated fMLP-stimulated spreading, migration and phosphorylation of endogenous Pyk2 without blocking adhesion of dHL-60 cells to fibrinogen. (biomedcentral.com)
  • Phosphorylation of a 72-kDa nucleoprotein (NP-72) in HL-60 cells is mediated by the double-stranded DNA-dependent protein kinase (DNA-PK). (semanticscholar.org)
  • Nuclear extracts prepared from human leukemic cells grown in the presence of cell differentiation-inducing agents showed a significant increase in the phosphorylation of a nuclear protein referred to as NP-72. (semanticscholar.org)
  • The decrease in transferrin receptor synthesis is a specific and early event that precedes the cessation of cell proliferation, differentiation, and the decrease in total protein synthesis. (elsevier.com)
  • This includes three proteins, whose expression was lower (tubulin β chain) or markedly higher (proliferating cell nuclear antigen and protein kinase C inhibitor protein 1) after administration of cells with Dau-conjugate vs free drug. (uni-konstanz.de)
  • The immune checkpoint programmed cell death protein 1 (PD-1, CD279) and its ligand PD-L1 (B7-H1, CD274) have rapidly taken center stage in tumor immunology. (haematologica.org)
  • In the present study, we found that the methyl ester derivative of indomethacin (M-IN) could more potently inhibit prostaglandin E 2 (PGE 2 ) and nitric oxide (NO) production and inducible nitric oxide synthase (iNOS) and cyclooxygenase 2 (COX 2) protein expression from lipopolysaccharide (LPS)-stimulated RAW 264.7 cells than indomethacin, similar to the effect of a non-steroidal anti-inflammatory drugs (NSAID). (elsevier.com)
  • After siRNA were transfected into HL60 cells, both GINS2 expression level of mRNA and protein in interfering group were down-regulated when compared with control groups. (medsci.org)
  • Cyclin D3 was expressed after protein kinase C was depleted, was maximal at 16 h, decreased at 24 h and had completely disappeared at 36 h after phorbol myristate acetate was added to HL-60 cells. (elsevier.com)
  • The human promyelocytic cell line HL-60 was used since peripheral blood neutrophils are terminally differentiated and do not actively synthesize protein. (uthscsa.edu)
  • Cells were disrupted by nitrogen cavitation and subcellular organelles in postnuclear supernatant separated on a discontinuous gradient of Percoll modified to resolve organelles important in protein synthesis. (uthscsa.edu)
  • The inhibitors of protein kinase C (PKC) and extracellular signal-regulated kinase (ERK), but not of phosphoinositide 3-kinase (PI3-K), inhibited the HL-60 differentiation induced by the extract in combination with ATRA or 1,25-(OH) 2 D 3 , signifying that PKC and ERK were involved in the cell differentiation enhancement by the extract. (elsevier.com)
  • The cells exhibited characteristics of mature granulocytes and a higher NBT-reducing ability, being a 2.6-fold increase in the rate of NBT-positive ratio of HL-60 cells within the As 2 O 3 treatment versus almost a 13-fold increase in the TMP + As 2 O 3 group. (scielo.br)
  • The cellular morphology changes from blastoid appearance to that of granulocytes and the cells acquire the ability to phagocytize. (pnas.org)
  • Treated cells cease to proliferate, acquire the ability to reduce Nitro Blue Tetrazolium dye, and undergo morphological changes typical of differentiated granulocytes. (biochemj.org)
  • Fifty-five serum samples were analyzed for the presence of functional antibody against seven pneumococcal serogroups or serotypes (4, 6B, 9V, 14, 18C, 19F, and 23F) by using differentiated HL-60 cells (granulocytes) and peripheral blood leukocytes (PBLs). (asm.org)
  • In our present work, as a model, we have used the HL-60 human promyelocytic cell line that can be differentiated into granulocytes. (nanopaprika.eu)
  • Deimination was investigated in human neutrophils and HL-60 cells differentiated into granulocytes. (jimmunol.org)
  • This study investigates anti-proliferative potency of several cat's claw preparations with different quantitative and qualitative alkaloid contents on HL-60 acute promyelocytic human cells by applying trypan blue exclusion and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide reduction assay (MTT). (nih.gov)
  • The effect of LA on cell proliferation of HL-60 cancer cells were determined by MTT assay. (aacrjournals.org)
  • The expression of Bcl-2 and Bax proteins in HL-60 cells were visualized by means of immunohistochemical assay and cell viability was determined upon treatment with DEVD-CHO and LA. Typical morphological changes including cell shrinkage, chromatin condensation and characteristic DNA ladder formation in agarose gel electrophoresis were observed. (aacrjournals.org)
  • We compared the effects of chemotactic stimuli and of inhibitors of signalling proteins on morphology, chemokinesis and chemotaxis of neutrophils and differentiated HL-60 cells using videomicroscopy and a filter assay for chemotaxis. (sigmaaldrich.com)
  • METHODS: MTT cell viability assay was used to verify the sensitization of DNR by quercetin in K562/ADM and HL 60/ADM cells,and determine the reverse concentration extent,confocal laser scanning microscope was used to observe the subcellular distribution of DNR in K562/ADM and HL 60/ADM cells,and relevant sensitive cell lines, K562/S and HL 60/S,before and after quercetin exposion. (cnki.com.cn)
  • Standardization of an opsonophagocytic assay for the measurement of functional antibody activity against Streptococcus pneumoniae using differentiated HL-60 cells. (asm.org)
  • Culturable cells aid in assay standardization and likely reduce donor-to-donor variability. (asm.org)
  • The inhibitory effects of quercetin and kaempferol on growth of HL-60 cells was assessed by MTT assay. (greenmedinfo.com)
  • The cytotoxic effect of these 2 drugs was analysed by single cell electrophoresis assay. (greenmedinfo.com)
  • Abstract Background We have investigated the potential anticancer effects of karanjin, a principal furanoflavonol constituent of the Chinese medicine Fordia cauliflora, using cytotoxic assay, cell cycle arrest, and induction of apoptosis in three human cancer cell lines (A549, HepG2 and HL-60 cells). (uga.edu)
  • Results MTT cytotoxic assay showed that karanjin could inhibit the proliferation and viability of all three cancer cells. (uga.edu)
  • Their cytotoxic activity using WST assay and DNA damage by agarosegel electrophoresis were also evaluated in a human leukemia HL-60 cell line. (waocp.org)
  • Oxidative stress was assessed by spectrophotometric measurements of MDA and GSH levels while genotoxicity was determined by single cell gel electrophoresis (Comet assay). (biomedcentral.com)
  • In these studies, we found that the hematopoietic isoform of Pyk2 is predominantly expressed in dimethyl sulfoxide (DMSO)-differentiated HL-60 (dHL60) cells. (biomedcentral.com)
  • Chemotactic peptide activation of human neutrophils and HL-60 cells. (jci.org)
  • As the phosphoinositide pool in human neutrophils is difficult to prelabel with [3H]myoinositol, experiments were also carried out in the cultured human promyelocytic leukemia cell line HL-60 after differentiation with dimethylsulfoxide. (jci.org)
  • We have carried out a detailed comparison of the motile properties of differentiated HL-60 cells and human peripheral blood neutrophils. (sigmaaldrich.com)
  • Chemotactic peptide induced a front-tail polarity in HL-60 cells comparable to that of neutrophils. (sigmaaldrich.com)
  • The cytokine interleukin-8 was in contrast clearly less effective in activating motile responses of differentiated HL-60 cells as compared to neutrophils. (sigmaaldrich.com)
  • Full activation of neutrophils by a soluble inflammatory stimulus requires a co-stimulatory signal initiated by integrin binding to endothelial cells or extracellular matrix proteins [ 2 , 3 ]. (biomedcentral.com)
  • Genetic modification of Pyk2 expression in mature cells such as neutrophils using current techniques has been largely unsuccessful. (biomedcentral.com)
  • Therefore in this study, we chose the differentiated HL60 cells as a model for human neutrophils to study the role of Pyk2 in neutrophil migration. (biomedcentral.com)
  • We observed rapid and robust H3 deimination in HL-60 cells exposed to LPS, TNF, lipoteichoic acid, f-MLP, or hydrogen peroxide, which are stimuli that activate neutrophils. (jimmunol.org)
  • In this study, we examine conditions that favor production of citrullinated histones in HL-60 cells and human blood neutrophils. (jimmunol.org)
  • Neutrophils are the most abundant white blood cells in humans. (ac.ir)
  • Cell line: HL60 (Human promyelocytic leukemia). (abcam.com)
  • This is a non-adherent human leukocyte cell line. (thermofisher.com)
  • The human promyelocytic leukemia cell line HL-60 can be induced to undergo morphological and functional differentiation in vitro by various low molecular weight compounds. (pnas.org)
  • In this study, we investigated ethyl gallate (EG) for possible proapoptotic effects in the human promyelocytic leukemia cell line, HL-60. (mdpi.com)
  • Lantadene A (LA, 22β-angeloyloxy-3-oxoolean-12-en-28-oic acid) a pentacyclic triterpenoid isolated from leaves of obnoxious weed Lantana camara L. was evaluated for apoptosis induction in human leukemia HL-60 cell line. (aacrjournals.org)
  • from human and equine sources in the northeastern Unites States were detected by PCR, isolated, and propagated in the HL-60 promyelocytic leukemia cell line. (asm.org)
  • We have identified a stable variant of the HL60 cell line, HL60var, which displays a nearly complete absence of staining with several mAb directed against sLex and/or sLex-related structures. (jimmunol.org)
  • The HL-60 cell line, derived from a patient with acute promyelocytic leukemia, is a widely used model system to study the cellular and molecular events involved in differentiation of leukemic cells. (lu.se)
  • article{3e126d9a-e1b1-40c7-9b5d-aee4b6cc122e, abstract = {The HL-60 cell line, derived from a patient with acute promyelocytic leukemia, is a widely used model system to study the cellular and molecular events involved in differentiation of leukemic cells. (lu.se)
  • The present study selected and characterized a multidrug‑resistant HL‑60 human acute promyelocytic leukemia cell line, HL‑60/RS, by exposure to stepwise incremental doses of doxorubicin. (spandidos-publications.com)
  • Proliferation of mitochondria during the cell cycle of the human cell line (HL-60). (rupress.org)
  • Use only Express Delivery (overnight) for cell line. (addexbio.com)
  • HL-60 is a promyelocytic cell line derived by S.J. Collins, et al. (addexbio.com)
  • The present study showed that CXCL-12 stimulation induced the rapid internalization of CXCR4 and facilitated the formation of lamellipodia and uropod in acute leukemia cell line HL-60. (bvsalud.org)
  • Analysis of 152 T cell clones derived from the peripheral blood of two patients with acute EBV-induced infectious mononucleosis (IM) yielded 11 highly suppressive clones that had no cytotoxic activity for the natural killer sensitive K562 cell line, an autologous EBV-infected cell line, or an allogeneic EBV-infected B cell line. (jci.org)
  • Thakur AK, Soni UK, Rai G, Chatterjee SS, Kumar V (2015) Andrographolide Modulate some Toll-like Receptors and Cytokines Expressions in HL-60 Cell Line. (medcraveonline.com)
  • Observations reported in this communication reveal that a non-cytotoxic concentration of andrographolide (10 µM) completely suppresses TLR-7 and TLR-8 expressions in HL-60 cells and has no effects on TLR-3 expressions in the cell line. (medcraveonline.com)
  • First-line treatment of AML consists of a combination of cytarabine and an anthracycline, and although this combination results in 60% to 80% complete remissions in newly diagnosed patients, most patients will relapse with resistant disease ( 1 ). (aacrjournals.org)
  • In order to characterize the growth pattern of the human promyelocytic leukaemia cell line HL60, its kinetic parameters were studied. (elsevier.com)
  • The HL60 cell line, with fully characterized kinetics, provides a useful tool for the in vitro study of substances which may affect human leukaemic myelopoietic proliferation. (elsevier.com)
  • Polli, E. E. / Growth pattern of the human promyelocytic leukaemia cell line HL60 . (elsevier.com)
  • The HL-60 cell line is a human leukemia cell line that has been used for laboratory research on blood cell formation and physiology. (wikipedia.org)
  • The cell line was derived from a 36-year-old woman who was originally reported to have acute promyelocytic leukemia at the MD Anderson Cancer Center. (wikipedia.org)
  • The HL-60 cultured cell line provides a continuous source of human cells for studying the molecular events of myeloid differentiation and the effects of physiologic, pharmacologic, and virologic elements on this process. (wikipedia.org)
  • Six clear fractions (A-F) observed on DEAE-cellulose chromatography were assayed for effects on cell growth, differentiation and apoptosis using the human leukemic HL-60 cell line. (waocp.org)
  • This study focused on whether LPS induced NETs release in vitro in the HL60 cell line. (ac.ir)
  • In this study, the HL60 cell line was used for culture and DMSO for induction and differentiation. (ac.ir)
  • The Eos-HL-60 line is a useful model for studying human eosinophil responses to cytokines. (ashpublications.org)
  • Colony and cluster formation was spontaneous but in the presence of medium conditioned by either human placental cells or the human monocyte-like cell line, GCT, Luxol-fast-blue positive colonies and clusters were detected at a higher frequency. (elsevier.com)
  • Moore, M. A. / Detection of Luxol-fast-blue positive cells in human promyelocytic leukemia cell line HL-60 . (elsevier.com)
  • A recent study revealed that differentiation may be associated with the induction of apoptosis, and thus, differentiation-inducing therapy may be useful in combination with intensive chemotherapy to increase the susceptibility of leukemia blast cells to drug-induced apoptosis (1). (scielo.br)
  • In summary, crocin inhibits the proliferation and tumorigenicity of HL-60 cells, which may be mediated by the induction of apoptosis and cell cycle arrest and the regulation of Bcl-2 and Bax expression. (hindawi.com)
  • Taken together, these results demonstrate that inhibition of CoA-IT can be linked to blockade of proliferation and the induction of apoptosis in HL-60 cells. (aspetjournals.org)
  • Our results demonstrate that growth arrest and the induction of differentiation by Sch 52900 is due to the induction of the cell cycle inhibitor p21(WAF) and an inhibition of the extracellular signal-regulated kinase (ERK) signaling pathway which leads to the activation of the transcription factor AP-1. (lu.se)
  • We investigated the relationship between p53‐dependent apoptosis and differentiation induction using human promyelocytic leukemia HL‐60 cells transfected with pMAMneo expression vectors containing dexamethasone‐inducible wild‐type p53 (wt‐p53) cDNA inserts. (elsevier.com)
  • Annexin-V FITC staining for apoptosis and flow cytometric assays indicated induction of apoptosis by fractions E and F up to 23.8 and 31.8% of cells. (waocp.org)
  • This is the first report demonstrating that ginsenoside Rh2 potently inhibits the proliferation of human promyelocytic HL-60 cells via the $G_1$ phase cell cycle arrest and differentiation induction. (koreascience.or.kr)
  • Surprisingly, a synergistic induction of differentiation was observed when HL-60 cells were treated with ATRA or 1,25-(OH) 2 D 3 and the extract. (elsevier.com)
  • Acteoside inhibits human promyelocytic HL-60 leukemia cell proliferation via inducing cell cycle arrest at G0/G1 phase and differentiation into monocyte," Carcinogenesis , vol. 28, no. 9, pp. 1928-1936, 2007. (hindawi.com)
  • IL-5 did not affect proliferation, differentiation, or enzyme release in promyelocytic HL-60 cells. (ashpublications.org)
  • Combination treatment of TMP with As 2 O 3 has significant synergistic effects on the proliferative inhibition of HL-60 cells. (scielo.br)
  • LA induced marked concentration and time dependant inhibition of cancer cell proliferation with IC 50 value of 19.8 ± 0.10 μg/ml following 48h incubation. (aacrjournals.org)
  • Inhibition of proliferation of estrogen receptor-positive MCF-7 human breast cancer cells by flavonoids in the presence and absence of excess estrogen. (koreascience.or.kr)
  • Translocation of membrane-associated phospholipid phosphatidylserines, changes in cell morphology, activation of caspases, and cleavage of PARP were concomitant with this inhibition. (frontiersin.org)
  • Further,incubation HL-60 cells with extract/fractions for 24h caused an inhibition of cell viability in a concentrationdependentmanner. (waocp.org)
  • The correlation between phenotypic and functional changes of HL-60 cells on the one hand and subcellular redistribution of PP1-like activity on the other suggest that the translocations of PP1α and PP1γ isozymes may contribute to the 1,25(OH) 2 D 3 -induced monocytic differentiation of HL-60 cells. (aacrjournals.org)
  • In conclusion, changes in the control of the cell cycle that characterize the onset of monocytic and neutrophil differentiation are only triggered in early G1, but CD11b expression can be initiated from most points in the cell cycle. (biomedsearch.com)
  • Acute myeloid leukemias (AML) are clonal malignancies characterized by increased numbers of immature myeloid progenitor cells arrested at different stages of granulocytic and monocytic differentiation. (aacrjournals.org)
  • abstract = "The effect of galangin, a flavonol component of India root spice and the 'herbal' medicine propolis, on HL-60 human leukaemia cell survival is characterised. (elsevier.com)
  • abstract = "Human promyelocytic leukemia HL-60 cells can be induced to apoptosis by treatment with phorbol myristate acetate. (elsevier.com)
  • abstract = "Differentiation of HL-60 cells toward the eosinophilic series has not been reported previously. (elsevier.com)
  • The quercetin and kaempferol induced G(2)/M arrest and apoptosis of HL-60 cells. (greenmedinfo.com)
  • These data suggest that cyclin D3 expression is important in the regulation of cell cycle arrest and apoptosis but not the expression of differentiated phenotype. (elsevier.com)
  • When HL-60 cells were exposed to 1.0% DMSO, a marked increase in Na+-dependent uridine transport occurred within 72 hr, a time preceding maximum granulocytic differentiation. (nus.edu.sg)
  • This study tested the hypothesis that Polycomb Repressive Complex 1 (PRC1) may play a negative role in the granulocytic differentiation of acute promyelocytic leukemia (APL) cells. (elsevier.com)
  • Upon gene silencing of PCGF2 with lentiviral short hairpin RNA, granulocytic differentiation was induced as assessed by differentiation marker gene expression, nitroblue tetrazolium staining, Wright-Giemsa staining, and cell cycle analysis. (elsevier.com)
  • Taken together, these results support the notion that down-regulation of PCGF2 is sufficient to induce granulocytic differentiation of HL-60 cells via de-repression of HOXA7 gene expression. (elsevier.com)
  • In conclusion, we report that PCGF2, a PRC1 gene, played a negative role in the granulocytic differentiation of human APL cells. (elsevier.com)
  • These results suggest that ERW is an antioxidant, and that ERW, in combination with GSH, has an enhanced apoptosis-inducing effect on HL-60 cells, which might be mediated through the mitochondria-dependent pathway. (go.jp)
  • Therapeutic strategies targeting the programmed cell death-ligand 1/programmed cell death-1 pathway have shown significant responses and good tolerability in solid malignancies. (haematologica.org)
  • Baicalein induces apoptosis through ROS-mediated mitochondrial dysfunction pathway in HL-60 cells. (koreascience.or.kr)
  • B5.2 The carbohydrate moiety of the CD22 antigen can be modulated by inhibitors of the glycosylation pathway," B-cell antigens-papers, 65-67. (freepatentsonline.com)
  • Taken together, our research demonstrated that ATO induces mitochondrial pathway of apoptosis in HL-60 cells. (biomedcentral.com)
  • To illustrate the value of such analysis, the approach is applied to hematopoietic differentiation in four well studied models (HL-60, U937, Jurkat, and NB4 cells). (pnas.org)
  • In this study, the antileukemic effects of three isomeric pregnadienedione steroids [i.e., cis -guggulsterone, trans -guggulsterone, and 16-dehydroprogesterone] were investigated in HL60 and U937 cells as well as in primary leukemic blasts in culture. (aacrjournals.org)
  • Our results show that all three compounds inhibited the proliferation of HL60 and U937 cells, with IC 50 s ranging from 3.6 to 10.9 μmol/L after treatment for 6 days. (aacrjournals.org)
  • Collectively, our results showed that EG induces apoptosis in HL-60 via mitochondrial-mediated pathways. (mdpi.com)
  • These findings provide mechanistic insight into a novel function of miR-101-3p in serum withdrawal-induced apoptosis triggered by activating two different intrinsic or mitochondrial apoptosis pathways, implicating miR-101-3p as a therapeutic target that limits endothelial cell death associated with vascular disorders. (nature.com)
  • What's more, ATM, CHK2, and P53 gene could involve in the pathogenic signaling pathways of HL60 cells when GINS2 gene was down-regulated. (medsci.org)
  • We describe a system for analysis of the intracellular pathways in the biosynthesis and packaging of functionally important proteins in human myeloid cells. (uthscsa.edu)
  • 1 The present work was performed to investigate the effects of ginsenoside Rh2 on proliferation, cell cycle-regulation and differentiation of human leukemia HL-60 cells as well as the underlying mechanisms for these effects. (koreascience.or.kr)
  • glaucocalyx, a traditional Chinese medicinal herb, which has been shown to inhibit tumor cell proliferation. (nih.gov)
  • Furthermore, HL-60 cells were xenografted into nude mice and treated by crocin, the tumor weight and size were calculated, and the expression of Bcl-2 and Bax in xenografts was detected by immunohistochemical staining. (hindawi.com)
  • In addition, crocin (6.25, 25 mg/kg) inhibited the tumor weight and size of HL-60 xenografts in nude mice, inhibited Bcl-2 expression, and increased Bax expression in xenografts. (hindawi.com)
  • Affordable & consistent controls from 50 tumor cell lines, ideal for IHC, in situ hybridization & NGS applications. (amsbio.com)
  • Catching up with solid tumor oncology: what is the evidence for a prognostic role of programmed cell death-ligand 1/programmed cell death-1 expression in B-cell lymphomas? (haematologica.org)
  • Determining patients who will benefit most from programmed cell death-ligand 1/programmed cell death-1-directed immunotherapy and whether programmed cell death-ligand 1/programmed cell death-1 are adequate prognostic markers becomes an increasingly important clinical question, especially as aberrant programmed cell death-ligand 1/programmed cell death-1 expression are key mediators of impaired anti-tumor immune responses in a range of B-cell lymphomas. (haematologica.org)
  • Baicalein, one of the major flavonoid in Scutellaria baicalensis, has been known for its effects on proliferation and apoptosis of many tumor cell lines. (koreascience.or.kr)
  • The latter mechanisms could be important to reduce the development of drug resistance by tumor cells. (springer.com)
  • The product of the p53 tumor‐suppressor gene has been shown to function in apoptosis and cell cycle regulation. (elsevier.com)
  • Leukemia is a malignant clone hematonosis deprived from hemopoietic stem cells, blood system malignant tumors account for about 7% of the new tumor patients each year. (medsci.org)
  • Neutrophil Extracellular Traps Sequester Circulating Tumor Cells and Promote Metastasis. (ac.ir)
  • By employing promoter based transcription factor binding site analysis, we found enrichment in the set of divergent genes, of transcription factors functionally linked to tumor progression, cell cycle, and development. (biomedcentral.com)
  • In particular, combination treatment using two chemotherapeutic agents at low concentrations has been reported to have improved cytotoxic effects on cancer cells with minimal side effects. (scielo.br)
  • Notably, crocin significantly inhibits the growth of cancer cells but has no effects on normal cells [ 4 ]. (hindawi.com)
  • The morphological effects of LA treated HL-60 cancer cells were observed under a fluorescence microscope. (aacrjournals.org)
  • Recent studies have suggested that cyclic AMP (cAMP) may be involved in regulation of cell growth and differentiation of cancer cells. (aspetjournals.org)
  • ET-18-O-CH3 (1-O-octadecyl-2-O-methyl-sn-glycero-3-phosphocholine) is an antiproliferative agent, blocking the growth of cancer cells both in vitro and in vivo. (aspetjournals.org)
  • Arecaceae) induces apoptosis of human colon cancer Ht-29 cells. (scialert.net)
  • The spice based herbal medicines and the constituents have been reported to inhibit the proliferation of cancer cells directly. (biomedcentral.com)
  • The in vitro cytostatic effect and cellular uptake of Dau═Aoa-LTVSPWY-NH2 conjugate were studied on various human cancer cell lines expressing different levels of ErbB2 receptor which could be targeted by the peptide. (uni-konstanz.de)
  • We found that the new daunomycin-peptide conjugate is highly cytostatic and could be taken up efficiently by the human cancer cells studied. (uni-konstanz.de)
  • CXCL-12 and its receptor CXCR4 participate in breast cancer and melanoma cell metastasis to bone and lymphoid nodes. (bvsalud.org)
  • More recently, resveratrol has been studied for its anti-tumorigenic characteristics, which consist of its ability to induce differentiation, stimulate apoptosis and arrest cell cycle progression in cancer cells. (viurrspace.ca)
  • Numerous preclinical trials conducted on human cancer models, such as uterine, colorectal and leukemia, have established that resveratrol induces apoptosis in these cells. (viurrspace.ca)
  • Bestwick, C & Milne, L 2006, ' Influence of galangin on HL-60 cell proliferation and survival ', Cancer Letters , vol. 243, no. 1, pp. 80-89. (elsevier.com)
  • Yano H, Mizoguchi A, Fukuda K, Haramaki M, Ogasawara S, Momosaki S, Kojiro M. The herbal medicine Sho-saiko-to inhibits proliferation of cancer cell lines by inducing apoptosis and arrest at the G0/G1 phase. (koreascience.or.kr)
  • Treatment with high extracellular concentrations of ascorbic acid alone directly induced cancer cell death. (greenmedinfo.com)
  • Conclusion Karanjin can induce cancer cell death through cell cycle arrest and enhance apoptosis. (uga.edu)
  • The Effects of Purified Artemia Extract Proteins on Proliferation, Differentiation and Apoptosis of Human Leukemic HL-60 Cells', Asian Pacific Journal of Cancer Prevention , 17(12), pp. 5139-5145. (waocp.org)
  • The effect of low-molecular-weight fraction of water-soluble extract (WS-LMF) of an edible algae, Susabi-nori (Porphyra yezoensis), on cell proliferation was examined using various cancer cell lines. (elsevier.com)
  • Cancer and stem/progenitor cells have telomerase activity to maintain and protect the chromosome ends, but lose this activity during differentiation. (cdc.gov)
  • 2015). 'In Vitro Antioxidant and Antiproliferative Activities of Novel Orange Peel Extract and It's Fractions on Leukemia HL-60 Cells', Asian Pacific Journal of Cancer Prevention , 16(16), pp. 7053-7060. (waocp.org)
  • Karanjin induced apoptosis in cancer cell lines. (biomedcentral.com)
  • Thus, a systems level view is necessary for understanding how a cell coordinates this process and for developing effective therapeutic strategies to treat diseases, such as cancer, in which differentiation plays a significant role. (biomedcentral.com)
  • Western blot analysis demonstrated that hematopoietic Pyk2 was predominantly expressed after HL60 cell differentiation. (biomedcentral.com)
  • Although many therapeutic advances such as combined chemotherapy and hematopoietic stem cell transplantation have been made to improve the survival rate of APL patients, a higher proportion of patients relapse and hence do not undergo complete remission. (biomedcentral.com)
  • HL-60 cell membranes enriched in marker enzyme activities of the endoplasmic reticulum and the plasma membrane possess a high-affinity binding site for [3H]Ins(1,4,5)P3 (KD = 22 nM). (biochemj.org)
  • Concomitant with HL-60 cell differentiation, the maximal [3H]Ins(1,4,5)P3 binding in membranes increases 3-4-fold, with no change in KD. (biochemj.org)
  • Histamine but not lipopeptides stimulated high-affinity GTPase of guanine-nucleotide-binding proteins in membranes of undifferentiated HL-60 cells. (uni-regensburg.de)
  • This change was attributable to a decrease in the number of transporter molecules as determined by the binding of [3H]nitrobenzylthioinosine to cell membranes. (nus.edu.sg)
  • CXCL-12 also induced CD44 translocation into the uropod, while CD44 remained evenly distributed on the untreated cell membranes . (bvsalud.org)
  • We examined cell viability, morphological changes, DNA content and fragmentation, and expression of apoptosis-related proteins for up to 48 h after EG treatment. (mdpi.com)
  • The results showed that EG induced morphological changes and DNA fragmentation and reduced HL-60 cell viability in a dose-dependent and time-dependent manner. (mdpi.com)
  • Continuous exposure of the pMAMneo/wt‐p53 transfectants to 1 μM, dexamethasone for more than 24 h caused overexpression of wt‐p53 followed by cell death with morphological changes typical of apoptosis. (elsevier.com)
  • This study was to investigate the mechanism of quercetin restoring subcellular distribution of daunorubicin (DNR)in multidrug resistant leukemia cell lines,K562/ADM and HL 60/ADM,and reversing their MDR. (cnki.com.cn)
  • RESULTS: Compared with K562/S and HL 60/S cells,20-40 μmol/L of quercetin in vitro remarkably enhanced the sensitivity of K562/ADM and HL 60/ADM cells to DNR,restore the subcellular distribution of DNR,so as to reverse MDR. (cnki.com.cn)
  • In the current study, we report that EM23, a natural sesquiterpene lactone isolated from EM, inhibits the proliferation of human chronic myeloid leukemia (CML) K562 cells and acute myeloid leukemia (AML) HL-60 cells by inducing apoptosis. (frontiersin.org)
  • Taken together, these results provide important insights into the anticancer activities of the EM component EM23 against human CML K562 cells and AML HL-60 cells. (frontiersin.org)
  • This study aimed to examine the effects of crocin on HL-60 cells in vitro and in vivo and investigate the underlying mechanisms. (hindawi.com)
  • In the present study, a series of experiments were performed to examine the effects of crocin on HL-60 cells in vitro and in vivo and investigate the underlying mechanisms. (hindawi.com)
  • An enhanced inhibitory effect on the viability of the HL-60 cells was observed after treatment with a combination of ERW with various concentrations of GSH, whereas no cytotoxic effect in normal peripheral blood mononuclear cells was observed. (go.jp)
  • Tanshinone IIA inhibits acute promyelocytic leukemia cell proliferation and induces their apoptosis in vivo. (greenmedinfo.com)