PeroxidasesHorseradish Peroxidase: An enzyme isolated from horseradish which is able to act as an antigen. It is frequently used as a histochemical tracer for light and electron microscopy. Its antigenicity has permitted its use as a combined antigen and marker in experimental immunology.Glutathione Peroxidase: An enzyme catalyzing the oxidation of 2 moles of glutathione in the presence of hydrogen peroxide to yield oxidized glutathione and water. EC 1.11.1.9.Peroxidase: A hemeprotein from leukocytes. Deficiency of this enzyme leads to a hereditary disorder coupled with disseminated moniliasis. It catalyzes the conversion of a donor and peroxide to an oxidized donor and water. EC 1.11.1.7.Cytochrome-c Peroxidase: A hemeprotein which catalyzes the oxidation of ferrocytochrome c to ferricytochrome c in the presence of hydrogen peroxide. EC 1.11.1.5.Ascorbate Peroxidases: Peroxidases that utilize ASCORBIC ACID as an electron donor to reduce HYDROGEN PEROXIDE to WATER. The reaction results in the production of monodehydroascorbic acid and DEHYDROASCORBIC ACID.Eosinophil Peroxidase: A 66-kDa peroxidase found in EOSINOPHIL granules. Eosinophil peroxidase is a cationic protein with a pI of 10.8 and is comprised of a heavy chain subunit and a light chain subunit. It possesses cytotoxic activity towards BACTERIA and other organisms, which is attributed to its peroxidase activity.Iodide Peroxidase: A hemeprotein that catalyzes the oxidation of the iodide radical to iodine with the subsequent iodination of many organic compounds, particularly proteins. EC 1.11.1.8.Hydrogen Peroxide: A strong oxidizing agent used in aqueous solution as a ripening agent, bleach, and topical anti-infective. It is relatively unstable and solutions deteriorate over time unless stabilized by the addition of acetanilide or similar organic materials.Guaiacol: An agent thought to have disinfectant properties and used as an expectorant. (From Martindale, The Extra Pharmacopoeia, 30th ed, p747)Catalase: An oxidoreductase that catalyzes the conversion of HYDROGEN PEROXIDE to water and oxygen. It is present in many animal cells. A deficiency of this enzyme results in ACATALASIA.Selenium: An element with the atomic symbol Se, atomic number 34, and atomic weight 78.96. It is an essential micronutrient for mammals and other animals but is toxic in large amounts. Selenium protects intracellular structures against oxidative damage. It is an essential component of GLUTATHIONE PEROXIDASE.Lactoperoxidase: An enzyme derived from cow's milk. It catalyzes the radioiodination of tyrosine and its derivatives and of peptides containing tyrosine.Peroxiredoxins: A family of ubiquitously-expressed peroxidases that play a role in the reduction of a broad spectrum of PEROXIDES like HYDROGEN PEROXIDE; LIPID PEROXIDES and peroxinitrite. They are found in a wide range of organisms, such as BACTERIA; PLANTS; and MAMMALS. The enzyme requires the presence of a thiol-containing intermediate such as THIOREDOXIN as a reducing cofactor.Lignin: The most abundant natural aromatic organic polymer found in all vascular plants. Lignin together with cellulose and hemicellulose are the major cell wall components of the fibers of all wood and grass species. Lignin is composed of coniferyl, p-coumaryl, and sinapyl alcohols in varying ratios in different plant species. (From Merck Index, 11th ed)Oxidation-Reduction: A chemical reaction in which an electron is transferred from one molecule to another. The electron-donating molecule is the reducing agent or reductant; the electron-accepting molecule is the oxidizing agent or oxidant. Reducing and oxidizing agents function as conjugate reductant-oxidant pairs or redox pairs (Lehninger, Principles of Biochemistry, 1982, p471).Superoxide Dismutase: An oxidoreductase that catalyzes the reaction between superoxide anions and hydrogen to yield molecular oxygen and hydrogen peroxide. The enzyme protects the cell against dangerous levels of superoxide. EC 1.15.1.1.Chloride Peroxidase: An enzyme that catalyzes the chlorination of a range of organic molecules, forming stable carbon-chloride bonds. EC 1.11.1.10.Basidiomycota: A phylum of fungi that produce their sexual spores (basidiospores) on the outside of the basidium. It includes forms commonly known as mushrooms, boletes, puffballs, earthstars, stinkhorns, bird's-nest fungi, jelly fungi, bracket or shelf fungi, and rust and smut fungi.Benzyl Alcohols: Alcohols derived from the aryl radical (C6H5CH2-) and defined by C6H5CHOH. The concept includes derivatives with any substituents on the benzene ring.Antioxidants: Naturally occurring or synthetic substances that inhibit or retard the oxidation of a substance to which it is added. They counteract the harmful and damaging effects of oxidation in animal tissues.Glutathione Reductase: Catalyzes the oxidation of GLUTATHIONE to GLUTATHIONE DISULFIDE in the presence of NADP+. Deficiency in the enzyme is associated with HEMOLYTIC ANEMIA. Formerly listed as EC 1.6.4.2.Oxidative Stress: A disturbance in the prooxidant-antioxidant balance in favor of the former, leading to potential damage. Indicators of oxidative stress include damaged DNA bases, protein oxidation products, and lipid peroxidation products (Sies, Oxidative Stress, 1991, pxv-xvi).Heme: The color-furnishing portion of hemoglobin. It is found free in tissues and as the prosthetic group in many hemeproteins.Peroxides: A group of compounds that contain a bivalent O-O group, i.e., the oxygen atoms are univalent. They can either be inorganic or organic in nature. Such compounds release atomic (nascent) oxygen readily. Thus they are strong oxidizing agents and fire hazards when in contact with combustible materials, especially under high-temperature conditions. The chief industrial uses of peroxides are as oxidizing agents, bleaching agents, and initiators of polymerization. (From Hawley's Condensed Chemical Dictionary, 11th ed)Glutathione: A tripeptide with many roles in cells. It conjugates to drugs to make them more soluble for excretion, is a cofactor for some enzymes, is involved in protein disulfide bond rearrangement and reduces peroxides.Histocytochemistry: Study of intracellular distribution of chemicals, reaction sites, enzymes, etc., by means of staining reactions, radioactive isotope uptake, selective metal distribution in electron microscopy, or other methods.Lipid Peroxidation: Peroxidase catalyzed oxidation of lipids using hydrogen peroxide as an electron acceptor.Spectrophotometry: The art or process of comparing photometrically the relative intensities of the light in different parts of the spectrum.Kinetics: The rate dynamics in chemical or physical systems.Phanerochaete: A genus of fungi in the family Corticiaceae, order Stereales, that degrades lignin. The white-rot fungus Phanerochaete chrysosporium is a frequently used species in research.Benzidines: Very toxic industrial chemicals. They are absorbed through the skin, causing lethal blood, bladder, liver, and kidney damage and are potent, broad-spectrum carcinogens in most species.Coprinus: A genus of black-spored basidiomycetous fungi of the family Coprinaceae, order Agaricales; some species are edible.Iodides: Inorganic binary compounds of iodine or the I- ion.Selenoproteins: Selenoproteins are proteins that specifically incorporate SELENOCYSTEINE into their amino acid chain. Most selenoproteins are enzymes with the selenocysteine residues being responsible for their catalytic functions.Lipid Peroxides: Peroxides produced in the presence of a free radical by the oxidation of unsaturated fatty acids in the cell in the presence of molecular oxygen. The formation of lipid peroxides results in the destruction of the original lipid leading to the loss of integrity of the membranes. They therefore cause a variety of toxic effects in vivo and their formation is considered a pathological process in biological systems. Their formation can be inhibited by antioxidants, such as vitamin E, structural separation or low oxygen tension.Wheat Germ Agglutinin-Horseradish Peroxidase Conjugate: The lectin wheatgerm agglutinin conjugated to the enzyme HORSERADISH PEROXIDASE. It is widely used for tracing neural pathways.Pleurotus: A genus of basidiomycetous fungi, family POLYPORACEAE, order POLYPORALES, that grows on logs or tree stumps in shelflike layers. The species P. ostreatus, the oyster mushroom, is a choice edible species and is the most frequently encountered member of the genus in eastern North America. (Alexopoulos et al., Introductory Mycology, 4th ed, p531)Selenious Acid: A selenium compound with the molecular formula H2SO3. It used as a source of SELENIUM, especially for patients that develop selenium deficiency following prolonged PARENTERAL NUTRITION.Free Radicals: Highly reactive molecules with an unsatisfied electron valence pair. Free radicals are produced in both normal and pathological processes. They are proven or suspected agents of tissue damage in a wide variety of circumstances including radiation, damage from environment chemicals, and aging. Natural and pharmacological prevention of free radical damage is being actively investigated.Malondialdehyde: The dialdehyde of malonic acid.Molecular Sequence Data: Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.Peroxiredoxin VI: A peroxiredoxin that is a cytosolic bifunctional enzyme. It functions as a peroxiredoxin via a single redox-active cysteine and also contains a Ca2+-independent acidic phospholipase A2 activity.Catalysis: The facilitation of a chemical reaction by material (catalyst) that is not consumed by the reaction.Thyroid Gland: A highly vascularized endocrine gland consisting of two lobes joined by a thin band of tissue with one lobe on each side of the TRACHEA. It secretes THYROID HORMONES from the follicular cells and CALCITONIN from the parafollicular cells thereby regulating METABOLISM and CALCIUM level in blood, respectively.3,3'-DiaminobenzidineAmino Acid Sequence: The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.Hydrogen-Ion Concentration: The normality of a solution with respect to HYDROGEN ions; H+. It is related to acidity measurements in most cases by pH = log 1/2[1/(H+)], where (H+) is the hydrogen ion concentration in gram equivalents per liter of solution. (McGraw-Hill Dictionary of Scientific and Technical Terms, 6th ed)Electron Spin Resonance Spectroscopy: A technique applicable to the wide variety of substances which exhibit paramagnetism because of the magnetic moments of unpaired electrons. The spectra are useful for detection and identification, for determination of electron structure, for study of interactions between molecules, and for measurement of nuclear spins and moments. (From McGraw-Hill Encyclopedia of Science and Technology, 7th edition) Electron nuclear double resonance (ENDOR) spectroscopy is a variant of the technique which can give enhanced resolution. Electron spin resonance analysis can now be used in vivo, including imaging applications such as MAGNETIC RESONANCE IMAGING.Peroxiredoxin III: A THIOREDOXIN-dependent hydroperoxidase that is localized in the mitochondrial matrix. The enzyme plays a crucial role in protecting mitochondrial components from elevated levels of HYDROGEN PEROXIDE.Selenoprotein P: An extracellular selenoprotein that contains most of the SELENIUM in PLASMA. Selenoprotein P functions as an antioxidant and appears to transport selenium from the LIVER to peripheral tissues.Isoenzymes: Structurally related forms of an enzyme. Each isoenzyme has the same mechanism and classification, but differs in its chemical, physical, or immunological characteristics.Hemeproteins: Proteins that contain an iron-porphyrin, or heme, prosthetic group resembling that of hemoglobin. (From Lehninger, Principles of Biochemistry, 1982, p480)Selenocysteine: A naturally occurring amino acid in both eukaryotic and prokaryotic organisms. It is found in tRNAs and in the catalytic site of some enzymes. The genes for glutathione peroxidase and formate dehydrogenase contain the TGA codon, which codes for this amino acid.Polyporaceae: A family of bracket fungi, order POLYPORALES, living in decaying plant matter and timber.Microscopy, Electron: Microscopy using an electron beam, instead of light, to visualize the sample, thereby allowing much greater magnification. The interactions of ELECTRONS with specimens are used to provide information about the fine structure of that specimen. In TRANSMISSION ELECTRON MICROSCOPY the reactions of the electrons that are transmitted through the specimen are imaged. In SCANNING ELECTRON MICROSCOPY an electron beam falls at a non-normal angle on the specimen and the image is derived from the reactions occurring above the plane of the specimen.Ascorbic Acid: A six carbon compound related to glucose. It is found naturally in citrus fruits and many vegetables. Ascorbic acid is an essential nutrient in human diets, and necessary to maintain connective tissue and bone. Its biologically active form, vitamin C, functions as a reducing agent and coenzyme in several metabolic pathways. Vitamin C is considered an antioxidant.Polyporales: An order of fungi in the phylum BASIDIOMYCOTA having macroscopic basidiocarps. The members are characterized by their saprophytic activities as decomposers, particularly in the degradation of CELLULOSE and LIGNIN. A large number of species in the order have been used medicinally. (From Alexopoulos, Introductory Mycology, 4th ed, pp504-68)Reactive Oxygen Species: Molecules or ions formed by the incomplete one-electron reduction of oxygen. These reactive oxygen intermediates include SINGLET OXYGEN; SUPEROXIDES; PEROXIDES; HYDROXYL RADICAL; and HYPOCHLOROUS ACID. They contribute to the microbicidal activity of PHAGOCYTES, regulation of signal transduction and gene expression, and the oxidative damage to NUCLEIC ACIDS; PROTEINS; and LIPIDS.Thiobarbituric Acid Reactive Substances: Low-molecular-weight end products, probably malondialdehyde, that are formed during the decomposition of lipid peroxidation products. These compounds react with thiobarbituric acid to form a fluorescent red adduct.Oxidoreductases: The class of all enzymes catalyzing oxidoreduction reactions. The substrate that is oxidized is regarded as a hydrogen donor. The systematic name is based on donor:acceptor oxidoreductase. The recommended name will be dehydrogenase, wherever this is possible; as an alternative, reductase can be used. Oxidase is only used in cases where O2 is the acceptor. (Enzyme Nomenclature, 1992, p9)Glucose Oxidase: An enzyme of the oxidoreductase class that catalyzes the conversion of beta-D-glucose and oxygen to D-glucono-1,5-lactone and peroxide. It is a flavoprotein, highly specific for beta-D-glucose. The enzyme is produced by Penicillium notatum and other fungi and has antibacterial activity in the presence of glucose and oxygen. It is used to estimate glucose concentration in blood or urine samples through the formation of colored dyes by the hydrogen peroxide produced in the reaction. (From Enzyme Nomenclature, 1992) EC 1.1.3.4.Cyanides: Inorganic salts of HYDROGEN CYANIDE containing the -CN radical. The concept also includes isocyanides. It is distinguished from NITRILES, which denotes organic compounds containing the -CN radical.Eosinophils: Granular leukocytes with a nucleus that usually has two lobes connected by a slender thread of chromatin, and cytoplasm containing coarse, round granules that are uniform in size and stainable by eosin.Enzymes, Immobilized: Enzymes which are immobilized on or in a variety of water-soluble or water-insoluble matrices with little or no loss of their catalytic activity. Since they can be reused continuously, immobilized enzymes have found wide application in the industrial, medical and research fields.Manganese: A trace element with atomic symbol Mn, atomic number 25, and atomic weight 54.94. It is concentrated in cell mitochondria, mostly in the pituitary gland, liver, pancreas, kidney, and bone, influences the synthesis of mucopolysaccharides, stimulates hepatic synthesis of cholesterol and fatty acids, and is a cofactor in many enzymes, including arginase and alkaline phosphatase in the liver. (From AMA Drug Evaluations Annual 1992, p2035)Glutathione Transferase: A transferase that catalyzes the addition of aliphatic, aromatic, or heterocyclic FREE RADICALS as well as EPOXIDES and arene oxides to GLUTATHIONE. Addition takes place at the SULFUR. It also catalyzes the reduction of polyol nitrate by glutathione to polyol and nitrite.Agaricales: An extensive order of basidiomycetous fungi whose fruiting bodies are commonly called mushrooms.tert-Butylhydroperoxide: A direct-acting oxidative stress-inducing agent used to examine the effects of oxidant stress on Ca(2+)-dependent signal transduction in vascular endothelial cells. It is also used as a catalyst in polymerization reactions and to introduce peroxy groups into organic molecules.Armoracia: A plant genus of the family BRASSICACEAE known for the root used in hot SPICES. It is also the source of HORSERADISH PEROXIDASE which is widely used in laboratories.ThyroglobulinThiocyanates: Organic derivatives of thiocyanic acid which contain the general formula R-SCN.Selenomethionine: Diagnostic aid in pancreas function determination.Oxygen: An element with atomic symbol O, atomic number 8, and atomic weight [15.99903; 15.99977]. It is the most abundant element on earth and essential for respiration.Ferrocyanides: Inorganic salts of the hypothetical acid ferrocyanic acid (H4Fe(CN)6).Substrate Specificity: A characteristic feature of enzyme activity in relation to the kind of substrate on which the enzyme or catalytic molecule reacts.Oxidants: Electron-accepting molecules in chemical reactions in which electrons are transferred from one molecule to another (OXIDATION-REDUCTION).Liver: A large lobed glandular organ in the abdomen of vertebrates that is responsible for detoxification, metabolism, synthesis and storage of various substances.Phenols: Benzene derivatives that include one or more hydroxyl groups attached to the ring structure.Laccase: A copper-containing oxidoreductase enzyme that catalyzes the oxidation of 4-benzenediol to 4-benzosemiquinone. It also has activity towards a variety of O-quinols and P-quinols. It primarily found in FUNGI and is involved in LIGNIN degradation, pigment biosynthesis and detoxification of lignin-derived products.Spectrum Analysis, Raman: Analysis of the intensity of Raman scattering of monochromatic light as a function of frequency of the scattered light.Pinocytosis: The engulfing of liquids by cells by a process of invagination and closure of the cell membrane to form fluid-filled vacuoles.Immunoenzyme Techniques: Immunologic techniques based on the use of: (1) enzyme-antibody conjugates; (2) enzyme-antigen conjugates; (3) antienzyme antibody followed by its homologous enzyme; or (4) enzyme-antienzyme complexes. These are used histologically for visualizing or labeling tissue specimens.Glutathione Disulfide: A GLUTATHIONE dimer formed by a disulfide bond between the cysteine sulfhydryl side chains during the course of being oxidized.Anisoles: A group of compounds that are derivatives of methoxybenzene and contain the general formula R-C7H7O.Spectrophotometry, Ultraviolet: Determination of the spectra of ultraviolet absorption by specific molecules in gases or liquids, for example Cl2, SO2, NO2, CS2, ozone, mercury vapor, and various unsaturated compounds. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)Sodium Selenite: The disodium salt of selenious acid. It is used therapeutically to supply the trace element selenium and is prepared by the reaction of SELENIUM DIOXIDE with SODIUM HYDROXIDE.Amitrole: A non-selective post-emergence, translocated herbicide. According to the Seventh Annual Report on Carcinogens (PB95-109781, 1994) this substance may reasonably be anticipated to be a carcinogen. (From Merck Index, 12th ed) It is an irreversible inhibitor of CATALASE, and thus impairs activity of peroxisomes.Erythrocytes: Red blood cells. Mature erythrocytes are non-nucleated, biconcave disks containing HEMOGLOBIN whose function is to transport OXYGEN.Benzene DerivativesThyroiditis, Autoimmune: Inflammatory disease of the THYROID GLAND due to autoimmune responses leading to lymphocytic infiltration of the gland. It is characterized by the presence of circulating thyroid antigen-specific T-CELLS and thyroid AUTOANTIBODIES. The clinical signs can range from HYPOTHYROIDISM to THYROTOXICOSIS depending on the type of autoimmune thyroiditis.Plants: Multicellular, eukaryotic life forms of kingdom Plantae (sensu lato), comprising the VIRIDIPLANTAE; RHODOPHYTA; and GLAUCOPHYTA; all of which acquired chloroplasts by direct endosymbiosis of CYANOBACTERIA. They are characterized by a mainly photosynthetic mode of nutrition; essentially unlimited growth at localized regions of cell divisions (MERISTEMS); cellulose within cells providing rigidity; the absence of organs of locomotion; absence of nervous and sensory systems; and an alternation of haploid and diploid generations.Bromides: Salts of hydrobromic acid, HBr, with the bromine atom in the 1- oxidation state. (From McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)Vitamin E: A generic descriptor for all TOCOPHEROLS and TOCOTRIENOLS that exhibit ALPHA-TOCOPHEROL activity. By virtue of the phenolic hydrogen on the 2H-1-benzopyran-6-ol nucleus, these compounds exhibit varying degree of antioxidant activity, depending on the site and number of methyl groups and the type of ISOPRENOIDS.Thioredoxins: Hydrogen-donating proteins that participates in a variety of biochemical reactions including ribonucleotide reduction and reduction of PEROXIREDOXINS. Thioredoxin is oxidized from a dithiol to a disulfide when acting as a reducing cofactor. The disulfide form is then reduced by NADPH in a reaction catalyzed by THIOREDOXIN REDUCTASE.Trametes: A genus of fungi in the family Coriolaceae.Chromatography, High Pressure Liquid: Liquid chromatographic techniques which feature high inlet pressures, high sensitivity, and high speed.Organoselenium Compounds: Organic compounds which contain selenium as an integral part of the molecule.Recombinant Proteins: Proteins prepared by recombinant DNA technology.Thioredoxin-Disulfide Reductase: A FLAVOPROTEIN enzyme that catalyzes the oxidation of THIOREDOXINS to thioredoxin disulfide in the presence of NADP+. It was formerly listed as EC 1.6.4.5Prostaglandins G: A group of physiologically active prostaglandin endoperoxides. They are precursors in the biosynthesis of prostaglandins and thromboxanes. Most frequently encountered member of this group is the prostaglandin G2.Spectrum Analysis: The measurement of the amplitude of the components of a complex waveform throughout the frequency range of the waveform. (McGraw-Hill Dictionary of Scientific and Technical Terms, 6th ed)Base Sequence: The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.Models, Chemical: Theoretical representations that simulate the behavior or activity of chemical processes or phenomena; includes the use of mathematical equations, computers, and other electronic equipment.Models, Molecular: Models used experimentally or theoretically to study molecular shape, electronic properties, or interactions; includes analogous molecules, computer-generated graphics, and mechanical structures.Sulfhydryl Compounds: Compounds containing the -SH radical.Plant Proteins: Proteins found in plants (flowers, herbs, shrubs, trees, etc.). The concept does not include proteins found in vegetables for which VEGETABLE PROTEINS is available.Plant Leaves: Expanded structures, usually green, of vascular plants, characteristically consisting of a bladelike expansion attached to a stem, and functioning as the principal organ of photosynthesis and transpiration. (American Heritage Dictionary, 2d ed)Phenylhydrazines: Diazo derivatives of aniline, used as a reagent for sugars, ketones, and aldehydes. (Dorland, 28th ed)Asparagus Plant: A plant genus in the family LILIACEAE (sometimes placed in Asparagaceae) that contains ECDYSTEROIDS and is an ingredient of Siotone. The shoots are used as a vegetable and the roots are used in FOLK MEDICINE.Time Factors: Elements of limited time intervals, contributing to particular results or situations.Coumaric Acids: Hydroxycinnamic acid and its derivatives. Act as activators of the indoleacetic acid oxidizing system, thereby producing a decrease in the endogenous level of bound indoleacetic acid in plants.Biocatalysis: The facilitation of biochemical reactions with the aid of naturally occurring catalysts such as ENZYMES.Phenol: An antiseptic and disinfectant aromatic alcohol.Binding Sites: The parts of a macromolecule that directly participate in its specific combination with another molecule.Gene Expression Regulation, Enzymologic: Any of the processes by which nuclear, cytoplasmic, or intercellular factors influence the differential control of gene action in enzyme synthesis.Sodium Azide: A cytochrome oxidase inhibitor which is a nitridizing agent and an inhibitor of terminal oxidation. (From Merck Index, 12th ed)Electrophoresis, Polyacrylamide Gel: Electrophoresis in which a polyacrylamide gel is used as the diffusion medium.Cloning, Molecular: The insertion of recombinant DNA molecules from prokaryotic and/or eukaryotic sources into a replicating vehicle, such as a plasmid or virus vector, and the introduction of the resultant hybrid molecules into recipient cells without altering the viability of those cells.Sequence Homology, Amino Acid: The degree of similarity between sequences of amino acids. This information is useful for the analyzing genetic relatedness of proteins and species.Diiodotyrosine: A product from the iodination of MONOIODOTYROSINE. In the biosynthesis of thyroid hormones, diiodotyrosine residues are coupled with other monoiodotyrosine or diiodotyrosine residues to form T4 or T3 thyroid hormones (THYROXINE and TRIIODOTHYRONINE).Staining and Labeling: The marking of biological material with a dye or other reagent for the purpose of identifying and quantitating components of tissues, cells or their extracts.
"Incorporation of Tellurocysteine into Glutathione Transferase Generates High Glutathione Peroxidase Efficiency". Angewandte ... tellurocysteine efficiently inhibited aminoacylation and increased the efficiency of glutathione peroxidase. L-Tellurocysteine ...
High-concentration H 2O 2 is referred to as "high-test peroxide" (HTP). It can be used either as a monopropellant (not mixed ... Enzymes that use or decompose hydrogen peroxide are classified as peroxidases. The boiling point of H 2O 2 has been ... Higher concentrations may be considered hazardous and are typically accompanied by a Material Safety Data Sheet (MSDS). In high ... In the presence of a reducing agent, high concentrations of H 2O 2 will react violently. High-concentration hydrogen peroxide ...
Toxicological studies have shown that safrole is a weak hepatocarcinogen at higher doses in rats and mice. Safrole requires ... PPA also causes a decrease in glutathione and Glutathione Peroxidase activity. The epoxide of allylcatechol may also be ... Wisneski, Harris H.; Yates, Ronald L.; Davis, Henry M. "High-performance liquid chromatographic-fluorometric determination of ... Safrole can be detected in undiluted liquid beverages and pharmaceutical preparations by injection onto a reversed-phase high- ...
The bladder also contains high levels of tissue peroxidase. Sudan I is genotoxic. It is also carcinogenic in rats. Comparisons ... In bladder tissue, CYP enzymes are not detectable, while there are relatively high levels of peroxidases expressed in these ... Significantly higher incidences of micro-nucleated immature erythrocytes (MNIME)were found at a dose of 150/mg/day or more. ... It is suggested that a CYP- or peroxidase-mediated activation of Sudan I or a combination of both mechanisms as an explanation ...
The risk is higher in those with antibodies against thyroid peroxidase. Subclinical hypothyroidism is estimated to affect ... In 2007, the British Thyroid Association stated that combined T4 and T3 therapy carried a higher rate of side effects and no ... In overt primary hypothyroidism, TSH levels are high and T4 and T3 levels are low. Overt hypothyroidism may also be diagnosed ... There is a much higher rate of thyroid disorders, the most common of which is hypothyroidism, in individuals with Down syndrome ...
May 2007). "High prevalence of thyroid peroxidase gene mutations in patients with thyroid dyshormonogenesis". Eur. J. ...
... glutathione peroxidases and glutaredoxins, as well as reacting directly with oxidants.[100] Due to its high concentration and ... There are at least four different glutathione peroxidase isozymes in animals.[154] Glutathione peroxidase 1 is the most ... Peroxidases. catalase. H. 2. O. Water. {\displaystyle {\ce {{\underset {Oxygen}{O2}}-,{\underset {Superoxide}{*O2^{-}}}-,[{\ce ... the glutathione S-transferases show high activity with lipid peroxides.[157] These enzymes are at particularly high levels in ...
A 'sandwich' is formed of solid-phase antibody, suPAR and peroxidase-conjugated antibody. The concentration (ng/mL plasma) of ... Performing the suPARnostic ELISA requires two antibodies with high specificity for suPAR. The blood plasma sample from the ... The advantages of using monoclonal antibodies compared to using polyclonal antibodies includes: High homogeneity, absence of ... indicating a potential infection or high level of inflammation. In this case, a patient's health is likely to worsen and he or ...
Glutathione peroxidase and similar enzymes then convert the H2O2 to water and dioxygen. Parts of the immune system of higher ...
Another achievement is the encapsulation of high amounts of essential PUFA of fish or plant origin combined with natural ... Shukla, V. K. S.; Jensen, E.; Clausen, J. (1978). "Serum fatty acids and peroxidase abnormalities in Batten's disease". Res. ... Recently, Shukla has developed a designer oil by the name of Nutridan, containing high amounts of essential polyunsaturated ... Shukla, V. K. S.; Srivastava, K. C.: Erythrocyte glutathione peroxidase and serum lipid pattern: A comparison between Indian ...
Relatively high amount of rutin increases thyroid iodide uptake in rats and decreases serum T3 and T4 level. The decreased ... hormone level can be explained by its inhibitory effect produced on Thyroid peroxidase enzyme (TPO). Rutin is also an ...
They have been found in the cell walls of most plants, but are present at higher levels in the grasses (Poaceae) and also sugar ... Peroxidases can produce dimers of ferulic acid, in the presence of hydrogen peroxide through radical polymerization. Diferulic ... Identification is often by high performance liquid chromatography with a UV detector or by LC-MS. Alternatively they can be ...
This enzyme also demonstrates glutathione-dependent peroxidase activity towards lipid hydroperoxides. Model organisms have been ... Gerdin AK (2010). "The Sanger Mouse Genetics Programme: High throughput characterisation of knockout mice". Acta ... a high-throughput mutagenesis project to generate and distribute animal models of disease to interested scientists - at the ... "Identification and characterization of a novel microsomal enzyme with glutathione-dependent transferase and peroxidase ...
It is commonly used as a substrate with hydrogen peroxide for a peroxidase enzyme (such as horseradish peroxidase) or alone ... The formal reduction potentials for ABTS are high enough for it to act as an electron donor for the reduction of oxo species ... Its use allows the reaction kinetics of peroxidases themselves to be followed. In this way it also can be used to indirectly ...
S. ostrea produced a higher number of laccase, lignin peroxidase and manganese peroxidase enzymes. the discoloration of the dye ... Reddy, B.R.; Praveen, K.; Usha, K.Y. (2012). Studies on Manganese Peroxidase of a mushroom - Stereum Ostrea Production, ... The study concluded that the crude culture filtrate of S. ostrea shows high antimicrobial activity. S. ostrea produces ... high. It is tough and inedible. It grows on tree bark. This fungus is native to North America, where it is widespread and grows ...
A peroxidase enzyme present in the inner cell wall plays a role in germination. Fuligo septica grows on rotten wood and plant ... Slime molds have a high resistance to toxic levels of metals; one author was prompted to write "The levels of Zn in Fuligo ... ISBN 1-58729-627-6. Setala A, Nuorteva P (1989). "High metal contents found in Fuligo septica L. Wiggers and some other slime ... Stempen H, Evans RC (1982). "Behavior of the inner wall layer of the germinating Fuligo septica spore: evidence of peroxidase ...
PBA has a high prevalence rate with estimates of 1.5-2 million cases in the United States alone. Multiple sclerosis is a ... Hashimoto's thyroiditis is associated with thyroid peroxidase and thyroglobulin autoantibodies Hashitoxicosis Hypothyroidism ... February 2002). "Higher prevalence of antibodies to Borrelia burgdorferi in psychiatric patients than in healthy subjects". The ... Tumors of the pituitary gland are fairly common in the general population with estimates ranging as high as 25%. Most tumors ...
Additionally, there was higher glutathione (GSH) peroxidase (GPx) activity when the PBMC were treated with 300 mg/dL of alcohol ... The humoral immune response of mice fed the L diet was found to be higher than that of mice fed the C, S and W diets. On the ... The humoral response of all mice fed the L diet was found to be higher than that of mice fed the C diet or non purified diet. A ... The immune response of mice fed the L diets, was found to be almost five times higher than that of mice fed the corresponding C ...
... causing a higher buildup of acetaldehyde than normally seen in other groups. The high concentration of acetaldehyde causes the ... Acetaldehyde may also influence glutathione peroxidase, a key antioxidant enzyme, and increases the susceptibility to oxidative ... High acetate levels cause adenosine to accumulate in many parts of the brain. But when the rats were given caffeine, which ... Women attain a higher blood alcohol concentration (BAC) than men at the same number of drinks. At equivalent BACs, men and ...
... where both high elasticity and high resilience of resilin play important roles due to the rapid stress-release cycles of ... Meanwhile, di- or tri-tyrosine crosslinking is formed by oxidative coupling, mediated by peroxidase, between tyrosine residuals ... A high resilience indicate that an sufficient amount of energy input can be stored in the material, and released afterwards. An ... With such high mechanical resilience, the properties of rec1-Resilin can be applied to other clinical applications within the ...
It also forms the dark-brown, water-soluble pigment melanin at high levels of metabolism during the fixation of nitrogen, which ... To do so, it produces three enzymes (catalase, peroxidase, and superoxide dismutase) to "neutralise" reactive oxygen species. ...
... is designed to be a hydrogen donor for peroxidase. Instead of an indicator, bromopyrogallol red is now used ... bisoxalato moiety was determined to increase the cell efficiency to a higher level. ... "Study and analytical application of bromopyrogallol red as a hydrogen donor substrate for peroxidase". Analytica Chimica Acta. ...
The primary antibody can be labeled using a small molecule which interacts with a high affinity binding partner that can be ... As previously described, enzymes such as horseradish peroxidase or alkaline phosphatase are commonly used to catalyse reactions ... Flow cytometry also has a high capital cost associated with the purchase of a flow cytometer. Western blotting allows the ... The biotin-streptavidin is one commonly used high affinity interaction. The primary antibody can be probed for using a broader ...
Addition of high-concentration hydrophobic drugs (certain antibiotics, diuretics) and high free fatty acids can cause elevated ... Heme can also come from myoglobin, found mostly in muscle, cytochromes, found mostly in mitochondria, catalase, peroxidase, and ... ALP is also present in bone and placental tissue, so it is higher in growing children (as their bones are being remodelled) and ... When the AST is higher than ALT, a muscle source of these enzymes should be considered. For example, muscle inflammation due to ...
... s enjoy few commercial applications, being limited by the high toxicity of selenium as well as the sensitivity of the Se ... Three enzymes found in mammals contain selenols at their active sites: glutathione peroxidase, iodothyronine deiodinase, and ...
Too high a dose of levothyroxine causes hyperthyroidism.[18] Overdose can result in heart palpitations, abdominal pain, nausea ... people with elevated thyroid peroxidase antibody titers, people with symptoms of hypothyroidism and TSH levels between 5-10 mIU ... As it is a medical emergency with a high mortality rate, it should be treated in the intensive care unit[11] with thyroid ... starting at higher doses may cause acute coronary syndrome or an arrhythmia.[11] ...
... to a pure oxygen atmosphere under conditions of nutrient starvation has been widely adopted to induce lignin peroxidase (LiP) ... 1963; The use of lead citrate at high pH as an electron-opaque stain in electron microscopy. J Cell Biol17:208-212[CrossRef] ... Keyword(s): cellulose , lignin peroxidase , LiP, lignin peroxidase , NBT, nitro blue tetrazolium , oxidant stress , ... 1991; Depolymerization of a synthetic lignin in vitro by lignin peroxidase. Enzyme Microb Technol13:15-18[CrossRef] ...
Thylakoid-bound ascorbate peroxidase had a high similarity to cytosolic ascorbate peroxidases, and the precursor contained a ... Thylakoid-bound ascorbate peroxidase had a high similarity to cytosolic ascorbate peroxidases, and the precursor contained a ... Keywords: Ascorbate peroxidase (EC 1.11.1.11); Chloroplasts; Oxidative stress; Putative membrane-spanning domain; Thylakoid- ... A cDNA for thylakoid-bound ascorbate peroxidase of pumpkin was cloned and characterized. ...
... the researchers identified the minimum amount of heating needed to inactivate both peroxidase and ascorbic-acid oxidase ... According to FDA Asparagus are a high fiber vegetable, low calories, free saturated fat, and sodium free, high in folate & ... Intake High Levels of Calcium and Vitamin D either From Food or Supplements Colon cancer begins in the large intestine (called ... Recent research has shown that a diet high in fiber is linked with a lower incidence of bowel cancer. It is one of the richest ...
My tsh level was 4.6 thyroid peroxidase 1425 antithyroglobulin ab 462 what does this mean? should i be concerned? going to dr ... high level thyroid peroxidase and antithyroglobulin ab. Hello, My tsh level was 4.6 thyroid peroxidase 1425 antithyroglobulin ... thyroid peroxidase 1425 antithyroglobulin ab 462 what does this mean? should i be concerned? going to dr next week and want to ... It means that you have high levels of thyroid antibodies, consistent with having Hashimotos Thyroiditis, which is the most ...
High prevalence of thyroid peroxidase antibodies in patients with alopecia areata.. Baars MP, Greebe RJ, Pop VJ. ...
Cain on high thyroid peroxidase antibodies levels: Hypo thryroidism does not usually affect parathyroid function. But it would ... High Hs-CRP, high AGAP, high total protein, globulin and auto basophil? ... How are high levels of thyroid peroxidase antibody treated (125)tsh (317)ft4(74) is armour thyroid safe im ttc? ... Free T3 (liothyronine) high, thyroid peroxidase ab greater than 1000, thyroglobulin ab elevated but TSH ultrasensitive normal ...
High-performance liquid chromatographic investigation of product formation in the horseradish peroxidase-enhanced ... High-performance liquid chromatographic investigation of product formation in the horseradish peroxidase-enhanced ...
In this work the thermal and high pressure inactivation of strawberry polyphenol oxidase and peroxidase was investigated so as ... Combined high pressure-thermal treatment at pressures ranging from 100 to 690 MPa, temperatures ranging from 24 to 90 °C and ... Strawberry polyphenol oxidase was found to be highly resistant to both thermal and high pressure inactivation. Thus in order to ... The inactivation kinetics of POD during combined high pressure-thermal processing was well described by first-order kinetics ...
... its precise localization and higher enzymatic activity, Plant Molecular Biology" on DeepDyve, the largest online rental service ... It displayed GSH-dependent peroxidase activity and exhibited the largest affinity to and the highest catalytic efficiency on ... It displayed GSH-dependent peroxidase activity and exhibited the largest affinity to and the highest catalytic efficiency on ... A Chinese cabbage cDNA with high sequence identity to phospholipid hydroperoxide glutathione peroxidases encodes a novel ...
Quantification of fibrin deposition in flowing blood with peroxidase- labeled fibrinogen. High shear rates induce decreased ... Quantification of fibrin deposition in flowing blood with peroxidase-labeled fibrinogen. High shear rates induce decreased ... Quantification of fibrin deposition in flowing blood with peroxidase- labeled fibrinogen. High shear rates induce decreased ... Quantification of fibrin deposition in flowing blood with peroxidase- labeled fibrinogen. High shear rates induce decreased ...
High Anti thyroid peroxidase antibodies. Tiredness, pain on right side stomach. Taking Thyrowell. Reason, treatment?. Ask a ... I haev T3, T4, TSH levels within limits bou I have high count of ANTI0 THYROID PEROXIDASE ANTIBODIES . I get tired easily. I ... High Anti thyroid peroxidase antibodies. Tiredness, pain on right side stomach. Taking Thyrowell. Reason, treatment?. ... Hi, I am Dr. Shehzad Topiwala (Endocrinologist). I will be looking into your question and guiding you through the process. ...
High-throughput Increase the total number of rows showing on this page using the pull-down located below the table, or use the ... non-selenium glutathione peroxidase activity, reduced glutathione peroxidase activity, selenium-glutathione peroxidase activity ... Gene Ontology Term: glutathione peroxidase activity. GO ID. GO:0004602 Aspect. Molecular Function. Description. Catalysis of ... glutathione:hydrogen-peroxide oxidoreductase activity, GSH peroxidase activity, ...
Disclaimer: (1) The information provided on this website is for educational purposes only and is not intended to diagnose or treat any disease. Please do not apply any of this information without first speaking with your doctor. (2) The Ruscio Institute is a participant in the Amazon Services LLC Associates Program, an affiliate advertising program designed to provide a means for us to earn fees by linking to Amazon.com and affiliated sites. (3) Amazon and the Amazon logo are trademarks of Amazon.com, Inc, or its affiliates.. ...
What are high thyroid peroxidase antibodies?. A: High thyroid peroxidase antibodies indicate that the patient has an autoimmune ... What are some causes for elevated thyroid peroxidase autoabs levels?. A: A positive thyroid peroxidase antibody test indicates ...
Crystallographic analyses of NADH peroxidase Cys42Ala and Cys42Ser mutants: active site structures, mechanistic implications, ... Resolution (High). Resolution (Low). Percent Possible (Observed). R Merge I (Observed). R Sym I (Observed). Net I Over Average ... Refinement High Resolution. Refinement Low Resolution. Cut-off Sigma (I). Cut-off Sigma (F). Number of Reflections (All). ... CRYSTALLOGRAPHIC ANALYSES OF NADH PEROXIDASE CYS42ALA AND CYS42SER MUTANTS: ACTIVE SITE STRUCTURE, MECHANISTIC IMPLICATIONS, ...
High Resolution Shell. Resolution (High). Resolution (Low). Percent Possible (All). R Merge I (Observed). R-Sym I (Observed). ... Resolution (High). Resolution (Low). Percent Possible (Observed). R Merge I (Observed). R Sym I (Observed). Net I Over Average ... Refinement High Resolution. Refinement Low Resolution. Cut-off Sigma (I). Cut-off Sigma (F). Number of Reflections (All). ... SPECIFICITY OF LIGAND BINDING TO A BURIED POLAR CAVITY AT THE ACTIVE SITE OF CYTOCHROME C PEROXIDASE (2-AMINO-4-METHYLTHIAZOLE) ...
High concentrations of phosphate severely decrease stability of horseradish peroxidase. In addition to biomedical applications ... Horseradish peroxidase is ideal in many respects for these applications because it is smaller, more stable, and less expensive ... Horseradish peroxidase is also commonly used in techniques such as ELISA and Immunohistochemistry due to its monomeric nature ... The enzyme horseradish peroxidase (HRP), found in the roots of horseradish, is used extensively in biochemistry applications. ...
This enzyme is also called long chain fatty acid peroxidase. MARTIN RO, STUMPF PK (1959). "Fat metabolism in higher plants. XII ... In enzymology, a fatty-acid peroxidase (EC 1.11.1.3) is an enzyme that catalyzes the chemical reaction palmitate + 2 H2O2 ⇌ {\ ... This enzyme belongs to the family of oxidoreductases, specifically those acting on a peroxide as acceptor (peroxidases). The ...
Find thyroid peroxidase information, treatments for thyroid peroxidase and thyroid peroxidase symptoms. ... MedHelps thyroid peroxidase Center for Information, Symptoms, Resources, Treatments and Tools for thyroid peroxidase. ... Thyroid Peroxidase Ab HIGH but T3 & T4 Normal High RBC COUNT! HELP Me PLEAE! - Thyroid Disorders Community ... Posts on thyroid peroxidase (30). I got my thyroid peroxidase and is 169, is that consider Hashimostos, - Thyroid Disorders ...
Read chapter 21 Oxidative Stress at High Altitudes and Effects of Vitamin E: This book reviews the research pertaining to ... Typical detoxification enzymes are superoxide dismutase, the catalases, and various peroxidases. If the organism is confronted ... 18 Fluid Metabolism at High Altitudes 331-356 * 19 Maintenance of Body Weight at High Altitudes: In Search of 500 kcal/day 357- ... High-Altitude Hypoxia. Aerobic energy supply is necessary for all higher life forms. In accordance, an inadequate oxygen supply ...
Horseradish Peroxidase Conjugated from Abcam,Rabbit polyclonal to Staphylococcus Enterotoxin A (HRP) ( for all tested ... Well BAC Prep; Ideal for ... 1.5ml 2xYT cultures; Provides high-quality ... sequencing for small to large ... which minimizes ... Rabbit Anti-Staphylococcus Enterotoxin A Polyclonal Antibody, Horseradish Peroxidase Conjugated from Abcam. ...
... Authorized Users Only. ... Lignin peroxidase (LiP) is a heme-containing oxidoreductase that oxidizes structurally diverse substrates in an H2O2-dependent ... We for the first-time applied flow cytometry for screening of any ligninolytic peroxidase library. Obtained results suggest ... and activity analysis revealed a significant increase in the percentage of cells expressing LiP variants with higher oxidative ...
High prevalence of thyroid peroxidase gene mutations in patients with thyroid dyshormonogenesis.. Avbelj M1, Tahirovic H, ... The high percentage of single allele mutations implied possible intronic or regulatory TPO gene mutations or monoallelic ... The results showed a higher prevalence of TPO gene mutations in thyroid dyshormonogenesis when compared with published studies ... Thyroid peroxidase gene (TPO) mutations are one of the most common causes of thyroid dyshormonogenesis. The aim of this study ...
Graphical abstract Editor, we provided image with high resolution. Please find it in a folder name "MIAC-D-18-00081" in the FTP ... Zhao K, Gu W, Zheng SS, Zhang CL, Xian YZ (2015) SDS-MoS2 nanoparticles as highly-efficient peroxidase mimetics for ... Guan JF, Peng J, Jin XY (2015) Synthesis of copper sulfide nanorods as peroxidase mimics for the colorimetric detection of ... Guo J, Wang Y, Zhao M (2018) 3D flower-like ferrous(II) phosphate nanostructures as peroxidase mimetics for sensitive ...
Glutathione peroxidase 4 (GPx4) has a high preference for lipid hydroperoxides; it is expressed in nearly every mammalian cell ... Glutathione peroxidase (GPx) (EC 1.11.1.9) is the general name of an enzyme family with peroxidase activity whose main ... glutathione peroxidase 4 (phospholipid hydroperoxidase) GPX5. Chr. 6 p21.32. glutathione peroxidase 5 (epididymal androgen- ... Glutathione peroxidase 2 is an intestinal and extracellular enzyme, while glutathione peroxidase 3 is extracellular, especially ...
  • There are 73 peroxidase genes in A.thaliana. (uniprot.org)
  • The position on the tomato map of genes affected by moderate and high impact mutations was also compared with that of known markers/QTLs (Quantitative Trait Loci) associated with reproductive and yield-related traits. (mdpi.com)
  • The candidate genes/QTLs regulating heat tolerance in the selected landrace E42 could be further investigated to better understand the genetic mechanisms controlling traits for high and stable yield trait under high temperatures. (mdpi.com)
  • We showed that with this strategy a large number of peroxidase genes can be mapped on the barley genome. (pubmedcentralcanada.ca)
  • There is evidence that defense-related genes like those encoding peroxidase (PR-9), superoxide dismutase and thaumatin-like protein (PR-5) are potential candidates to explain quantitative resistance to plant pathogens , . (pubmedcentralcanada.ca)
  • Analysis of the genome of C. thermophilum [ 1 ] mainly focused on the presence of genes coding for nucleoporins of high thermal stability, whereas the draft genome of Chaetomium globosum [ 2 ] was mainly asked for diverse genes coding cellulolytic pathways. (biomedcentral.com)
  • Bloodstream Trypanosoma brucei in which the genes for cytosolic lipid hydroperoxide-detoxifying peroxidases have been knocked out undergo an extremely rapid membrane peroxidation and lyse within less than two hours when they are cultured without an exogenous antioxidant. (prolekare.cz)
  • The effect of 26,113 mutations on proteins' structure was investigated and it was discovered that 37 had a high impact on the structure of 34 proteins of which some are putatively involved in responses to high temperatures. (mdpi.com)
  • GE Healthcare HisTrap™ HP Prepacked Columns are prepacked with Ni Sepharose™ High Performance and designed for simple, high-resolution purification of histidine-tagged proteins by immobilized metal ion affinity chromatography (IMAC). (fishersci.com)
  • Higher plants possess a large multigene family encoding secreted class III peroxidase ( Prx ) proteins. (pubmedcentralcanada.ca)
  • Indeed, some proteins secreted by the actinomycetes Streptomyces thermoviolaceus ( 17 ), Streptomyces viridosporus T7A ( 6 , 26 , 27 ), and Thermomonospora fusca BD25 (recently reclassified as Thermobifida fusca [ 35 ]) ( 3 , 28 ) have low peroxidase activity and have been isolated and partially characterized. (asm.org)
  • The peroxidase-like proteins from S. thermoviolaceus and S. viridosporus T7A were assigned as heme peroxidases on the basis of their optical spectra. (asm.org)
  • Peroxidase conjugates of other targeting proteins such as lectins and receptor ligands are likely to be suitable for labeling targets, as is endogenous peroxidase activity. (thermofisher.com)
  • Horseradish peroxidase catalyses the oxidation of luminol to 3-aminophthalate via several intermediates. (wikipedia.org)
  • New technologies for waste disposal that use high-temperature incineration and chemical decomposition (e.g., base-catalyzed dechlorination, UV oxidation) have evolved. (hindawi.com)
  • We concluded that extracellular H 2 O 2 produced by the fungus during oxidation of aromatic metabolites is responsible for inactivation of the peroxidase and that the enzyme can protect itself in the presence of different reducing substrates. (asm.org)
  • As peroxidases (E.C. 1.11.1.7, doador H 2 O 2 oxidorredutase) catalisam a redução do peróxido de hidrogênio ou outro peróxido orgânico, enquanto um doador de elétrons é oxidado. (scielo.br)
  • Liu, Jin-Yuan 2006-08-30 00:00:00 A novel cDNA of phospholipid hydroperoxide glutathione peroxidase (PHGPx), which encodes a functional protein capable of complementing the yeast PHGHX-deletion mutant, was recently discovered in radish (Raphanus sativus) and designated as RsPHGPx [Yang X-D, Li W-J, Liu J-Y (2005) Biochim Biophys Acta 1728:199- (deepdyve.com)
  • Another peroxidase of barley, HvPrx8 is pathogen-induced at the mRNA as well as protein level . (pubmedcentralcanada.ca)
  • Restoring redox balance enhances contractility in heart trabeculae from type 2 diabetic rats exposed to high glucose. (abcam.com)
  • Turkovskaya, O. 2017-03-05 00:00:00 From the roots and root exudates of 3-week-old plants of alfalfa (Medicago sativa L.), anionic and cationic peroxidases differing in principal physicochemical and catalytic properties were isolated and purified. (deepdyve.com)
  • Previously, we identified pneumococcal thiol peroxidase (TpxD) as the key enzyme for enzymatic removal of H 2 O 2 , and showed that TpxD synthesis is up-regulated upon exposure to H 2 O 2 . (frontiersin.org)
  • In addition, it acts as a versatile peroxidase with a pH optimum in the range 5.0-5.5 using both one-electron [2,2′-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) o -dianisidine, pyrogallol or guaiacol] and two-electron (Br − , I − or ethanol) donors. (biochemj.org)
  • Specific isoenzymes of cell wall-localized peroxidases are widely believed to be responsible for the final enzymatic step in lignification: the oxidative dehydrogenation of monolignols, which leads to free radical polymerization ( Higuchi, 1985 ). (plantphysiol.org)
  • The methods utilizes one or two different types of oxidizing agents which selectively oxidize low-molecular weight reducing substances and high-molecular weight (mainly hemoglobin) reducing substances in blood samples, coupled with enzymatic reactions catalyzed by proteases, fructosyl amino acid oxidase, and peroxidase. (google.com.au)
  • After two short postdoctoral internships, her current scientific interest is the development of enzymatic methodologies for the synthesis of high added-value compounds and natural products as well, together with the use of new trends in organocatalysis for the same purposes. (wiley.com)
  • The prevalence of TPOAb is even higher in women with a history of recurrent pregnancy loss, at around 17-33%, and in women with a history of subfertility, at around 10-31% [ 4 ]. (hindawi.com)
  • The prevalence of TPOAb is much higher in women with a history of recurrent miscarriage and subfertility. (hindawi.com)
  • However, molecular models built using LiP, MnP and other peroxidases as templates show the presence of a manganese-binding site in the proximity of the haem ring, on the side of the two haem propionate groups, in a manner similar to that found in MnP [ 6 ]. (portlandpress.com)
  • All five peroxidases were strongly glycosylated (3.6% to 4.9% N -glucosamine), with apparent molecular masses between 46 and 54 kD and pI values between pH 3.1 and 3.8. (plantphysiol.org)
  • This phenomenon complicates the determination of physiological roles for peroxidases when using a molecular biology approach. (plantphysiol.org)
  • In addition, maspin expression led to a dramatic reduction in the release of active uPA, both high molecular weight and the low molecular weight, into the conditioned culture medium. (aacrjournals.org)
  • CONCLUSIONS Pregnant women with IDDM who have a positive test for TPO-Abs before gestation have poorer glucose control and a high prevalence of hypothyroidism. (diabetesjournals.org)
  • Horseradish peroxidase is also commonly used in techniques such as ELISA and Immunohistochemistry due to its monomeric nature and the ease with which it produces coloured products. (wikipedia.org)
  • Horseradish peroxidase is widely used as a label for immunoglobulins in many different immunochemistry applications including ELISA, immunoblotting and immunohistochemistry. (sigmaaldrich.com)
  • The kit provides the enzyme, accessory reagents and protocol to easily produce high-performance conjugates for Western blotting, ELISA and other detection techniques. (thermofisher.com)
  • The Wash and Stop Solution for ELISA without Sulfuric Acid contains all of the reagents necessary for preparing wash buffer (10X PBS and Tween20) and a non-sulfuric acid formulation stop solution for peroxidase-based ELISA or immunoassay applications, including Takara Bio's EIA kits. (clontech.com)
  • The ELISA Stop Solution included with this product is intended for stopping the colorimetric reaction of 3,3′,5,5′- tetramethylbenzidine (TMB) substrate in horseradish peroxidase (HRP)-based ELISA detection. (clontech.com)
  • High quality, reliable stock solutions for preparing wash buffers for ELISA or immunoassay protocols. (clontech.com)
  • The peroxidase micropolymers of the ImmPRESS™ HRP polymer reagent limit steric interference and provide enhanced accessibility to the target, avoiding the disadvantages of other polymer systems that use large dextrans or other macromolecules as backbones. (vectorlabs.com)
  • The highly active peroxidase micropolymers of the ImmPRESS Detection System provide outstanding sensitivity, limit steric interference, and enhance accessibility. (vectorlabs.com)
  • This Vector M.O.M. ImmPRESS Kit contains our proprietary M.O.M. Mouse Ig Blocking Reagent paired with a specialized, ready-to-use, one-step M.O.M. ImmPRESS Peroxidase Polymer reagent. (vectorlabs.com)
  • The peroxidase was inactivated after 4 hours at 80 °C and after 3 minutes at 96 °C. This enzyme can possibly be used as a diagnostic reagent, biosensor and for other analytical methods in several fields of Sciences. (scielo.br)
  • In the presence of a synthetic mediator, purified peroxidases can oxidize phenanthrene and its derivatives, including potential microbial metabolites of polycyclic aromatic hydrocarbons (PAH). (deepdyve.com)
  • In the course of evolution, life found a way to use the high-energy potential of reducing oxygen to water within the mitochondrial respiratory chain. (nap.edu)