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HEPESBuffersTromethamineAmino Acids, NeutralBicarbonatesHydrogen-Ion Concentration4,4'-Diisothiocyanostilbene-2,2'-Disulfonic AcidFraxinusLichensGermanyPhysician AssistantsSaltsSodiumPhenolsulfonphthaleinPhenolphthaleinsPhenolCulture MediaCell LineCricetinaeCarbonic Anhydrase ICatalogs, LibraryDNA DamageCatalogs as TopicProstaglandins F, SyntheticPhysical FitnessOrganic ChemicalsNanoparticlesMultiple MyelomaPublicationsGlutamineClick ChemistryPeriodicals as TopicBibliometricsJournal Impact FactorContainment of BiohazardsSurgery, VeterinaryAutonomic PathwaysSinoatrial NodeSearch EngineBiofuelsMolecular BiologyTerminology as TopicNuclear Magnetic Resonance, BiomolecularProtonsProton Pumps
HEPES: A dipolar ionic buffer.Buffers: A chemical system that functions to control the levels of specific ions in solution. When the level of hydrogen ion in solution is controlled the system is called a pH buffer.Tromethamine: An organic amine proton acceptor. It is used in the synthesis of surface-active agents and pharmaceuticals; as an emulsifying agent for cosmetic creams and lotions, mineral oil and paraffin wax emulsions, as a biological buffer, and used as an alkalizer. (From Merck, 11th ed; Martindale, The Extra Pharmacopoeia, 30th ed, p1424)Amino Acids, Neutral: Amino acids with uncharged R groups or side chains.Bicarbonates: Inorganic salts that contain the -HCO3 radical. They are an important factor in determining the pH of the blood and the concentration of bicarbonate ions is regulated by the kidney. Levels in the blood are an index of the alkali reserve or buffering capacity.Hydrogen-Ion Concentration: The normality of a solution with respect to HYDROGEN ions; H+. It is related to acidity measurements in most cases by pH = log 1/2[1/(H+)], where (H+) is the hydrogen ion concentration in gram equivalents per liter of solution. (McGraw-Hill Dictionary of Scientific and Technical Terms, 6th ed)4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid: An inhibitor of anion conductance including band 3-mediated anion transport.Fraxinus: A plant genus of the family OLEACEAE. Members contain secoiridoid glucosides.Lichens: Any of a group of plants formed by a symbiotic combination of a fungus with an algae or CYANOBACTERIA, and sometimes both. The fungal component makes up the bulk of the lichen and forms the basis for its name.GermanyPhysician Assistants: Health professionals who practice medicine as members of a team with their supervising physicians. They deliver a broad range of medical and surgical services to diverse populations in rural and urban settings. Duties may include physical exams, diagnosis and treatment of disease, interpretation of tests, assist in surgery, and prescribe medications. (from http://www.aapa.orglabout-pas accessed 2114/2011)Salts: Substances produced from the reaction between acids and bases; compounds consisting of a metal (positive) and nonmetal (negative) radical. (Grant & Hackh's Chemical Dictionary, 5th ed)Sodium: A member of the alkali group of metals. It has the atomic symbol Na, atomic number 11, and atomic weight 23.Phenolsulfonphthalein: Red dye, pH indicator, and diagnostic aid for determination of renal function. It is used also for studies of the gastrointestinal and other systems.Phenolphthaleins: A family of 3,3-bis(p-hydroxyphenyl)phthalides. They are used as CATHARTICS, indicators, and COLORING AGENTS.Phenol: An antiseptic and disinfectant aromatic alcohol.Culture Media: Any liquid or solid preparation made specifically for the growth, storage, or transport of microorganisms or other types of cells. The variety of media that exist allow for the culturing of specific microorganisms and cell types, such as differential media, selective media, test media, and defined media. Solid media consist of liquid media that have been solidified with an agent such as AGAR or GELATIN.Cell Line: Established cell cultures that have the potential to propagate indefinitely.Cricetinae: A subfamily in the family MURIDAE, comprising the hamsters. Four of the more common genera are Cricetus, CRICETULUS; MESOCRICETUS; and PHODOPUS.Carbonic Anhydrase I: A cytosolic carbonic anhydrase isoenzyme primarily expressed in ERYTHROCYTES, vascular endothelial cells, and the gastrointestinal mucosa. EC 4.2.1.-Catalogs, LibraryDNA Damage: Injuries to DNA that introduce deviations from its normal, intact structure and which may, if left unrepaired, result in a MUTATION or a block of DNA REPLICATION. These deviations may be caused by physical or chemical agents and occur by natural or unnatural, introduced circumstances. They include the introduction of illegitimate bases during replication or by deamination or other modification of bases; the loss of a base from the DNA backbone leaving an abasic site; single-strand breaks; double strand breaks; and intrastrand (PYRIMIDINE DIMERS) or interstrand crosslinking. Damage can often be repaired (DNA REPAIR). If the damage is extensive, it can induce APOPTOSIS.Catalogs as Topic: Ordered compilations of item descriptions and sufficient information to afford access to them.Prostaglandins F, Synthetic: Analogs or derivatives of prostaglandins F that do not occur naturally in the body. They do not include the product of the chemical synthesis of hormonal PGF.Physical Fitness: The ability to carry out daily tasks and perform physical activities in a highly functional state, often as a result of physical conditioning.Organic Chemicals: A broad class of substances containing carbon and its derivatives. Many of these chemicals will frequently contain hydrogen with or without oxygen, nitrogen, sulfur, phosphorus, and other elements. They exist in either carbon chain or carbon ring form.Nanoparticles: Nanometer-sized particles that are nanoscale in three dimensions. They include nanocrystaline materials; NANOCAPSULES; METAL NANOPARTICLES; DENDRIMERS, and QUANTUM DOTS. The uses of nanoparticles include DRUG DELIVERY SYSTEMS and cancer targeting and imaging.Multiple Myeloma: A malignancy of mature PLASMA CELLS engaging in monoclonal immunoglobulin production. It is characterized by hyperglobulinemia, excess Bence-Jones proteins (free monoclonal IMMUNOGLOBULIN LIGHT CHAINS) in the urine, skeletal destruction, bone pain, and fractures. Other features include ANEMIA; HYPERCALCEMIA; and RENAL INSUFFICIENCY.Publications: Copies of a work or document distributed to the public by sale, rental, lease, or lending. (From ALA Glossary of Library and Information Science, 1983, p181)Glutamine: A non-essential amino acid present abundantly throughout the body and is involved in many metabolic processes. It is synthesized from GLUTAMIC ACID and AMMONIA. It is the principal carrier of NITROGEN in the body and is an important energy source for many cells.Click Chemistry: Organic chemistry methodology that mimics the modular nature of various biosynthetic processes. It uses highly reliable and selective reactions designed to "click" i.e., rapidly join small modular units together in high yield, without offensive byproducts. In combination with COMBINATORIAL CHEMISTRY TECHNIQUES, it is used for the synthesis of new compounds and combinatorial libraries.Periodicals as Topic: A publication issued at stated, more or less regular, intervals.Bibliometrics: The use of statistical methods in the analysis of a body of literature to reveal the historical development of subject fields and patterns of authorship, publication, and use. Formerly called statistical bibliography. (from The ALA Glossary of Library and Information Science, 1983)Journal Impact Factor: A quantitative measure of the frequency on average with which articles in a journal have been cited in a given period of time.Containment of Biohazards: Provision of physical and biological barriers to the dissemination of potentially hazardous biologically active agents (bacteria, viruses, recombinant DNA, etc.). Physical containment involves the use of special equipment, facilities, and procedures to prevent the escape of the agent. Biological containment includes use of immune personnel and the selection of agents and hosts that will minimize the risk should the agent escape the containment facility.Surgery, Veterinary: A board-certified specialty of VETERINARY MEDICINE, requiring at least four years of special education, training, and practice of veterinary surgery after graduation from veterinary school. In the written, oral, and practical examinations candidates may choose either large or small animal surgery. (From AVMA Directory, 43d ed, p278)Autonomic Pathways: Nerves and plexuses of the autonomic nervous system. The central nervous system structures which regulate the autonomic nervous system are not included.Sinoatrial Node: The small mass of modified cardiac muscle fibers located at the junction of the superior vena cava (VENA CAVA, SUPERIOR) and right atrium. Contraction impulses probably start in this node, spread over the atrium (HEART ATRIUM) and are then transmitted by the atrioventricular bundle (BUNDLE OF HIS) to the ventricle (HEART VENTRICLE).Search Engine: Software used to locate data or information stored in machine-readable form locally or at a distance such as an INTERNET site.Biofuels: Hydrocarbon-rich byproducts from the non-fossilized BIOMASS that are combusted to generate energy as opposed to fossilized hydrocarbon deposits (FOSSIL FUELS).Molecular Biology: A discipline concerned with studying biological phenomena in terms of the chemical and physical interactions of molecules.Terminology as Topic: The terms, expressions, designations, or symbols used in a particular science, discipline, or specialized subject area.Nuclear Magnetic Resonance, Biomolecular: NMR spectroscopy on small- to medium-size biological macromolecules. This is often used for structural investigation of proteins and nucleic acids, and often involves more than one isotope.Protons: Stable elementary particles having the smallest known positive charge, found in the nuclei of all elements. The proton mass is less than that of a neutron. A proton is the nucleus of the light hydrogen atom, i.e., the hydrogen ion.Proton Pumps: Integral membrane proteins that transport protons across a membrane. This transport can be linked to the hydrolysis of ADENOSINE TRIPHOSPHATE. What is referred to as proton pump inhibitors frequently is about POTASSIUM HYDROGEN ATPASE.

Transcriptional regulation of the esp genes of enterohemorrhagic Escherichia coli. (1/141)

We have determined that the genes encoding the secreted proteins EspA, EspD, and EspB of enterohemorrhagic Escherichia coli (EHEC) are organized in a single operon. The esp operon is controlled by a promoter located 94 bp upstream from the ATG start codon of the espA gene. The promoter is activated in the early logarithmic growth phase, upon bacterial contact with eukaryotic cells and in response to Ca2+, Mn2+, and HEPES. Transcription of the esp operon seems to be switched off in tightly attached bacteria. The activation process is regulated by osmolarity (induction at high osmolarities), modulated by temperature, and influenced by the degree of DNA supercoiling. Transcription is sigmaS dependent, and the H-NS protein contributes to its fine tuning. Identification of the factors involved in activation of the esp operon and the signals responsible for modulation may facilitate understanding of the underlying molecular events leading to sequential expression of virulence factors during natural infections caused by EHEC.  (+info)

Generation of intracellular pH gradients in single cardiac myocytes with a microperfusion system. (2/141)

This study describes the use of a microperfusion system to create rapid, large regional changes in intracellular pH (pH(i)) within single ventricular myocytes. The spatial distribution of pH(i) in single myocytes was measured with seminaphthorhodafluor-1 fluorescence using confocal imaging. Changes in pH(i) were induced by local external application of NH(4)Cl, CO(2), or sodium propionate. Local application was achieved by simultaneously directing two parallel square microstreams, each 275 microm wide, over a single myocyte oriented perpendicular to the direction of flow. One stream contained the control solution, and the other contained a weak acid or base. End-to-end, stable pH(i) gradients as large as 1 pH unit were readily created with this technique. This result indicates that pH within a single cardiac cell may not always be spatially uniform, particularly when weak acid or base gradients are present, which can occur, for example, in regional myocardial ischemia. The microperfusion method should be useful for studying the effects of localized acidosis on myocyte function, estimating intracellular ion diffusion rates, and, possibly, inducing regional changes in other important intracellular ions.  (+info)

Augmentation of L-type calcium current by hypoxia in rabbit carotid body glomus cells: evidence for a PKC-sensitive pathway. (3/141)

Previous studies have suggested that voltage-gated Ca(2+) influx in glomus cells plays a critical role in sensory transduction at the carotid body chemoreceptors. The purpose of the present study was to determine the effects of hypoxia on the Ca(2+) current in glomus cells and to elucidate the underlying mechanism(s). Experiments were performed on freshly dissociated glomus cells from rabbit carotid bodies. Ca(2+) current was monitored using the whole cell configuration of the patch-clamp technique, with Ba(2+) as the charge carrier. Hypoxia (pO(2) = 40 mmHg) augmented the Ca(2+) current by 24 +/- 3% (n = 42, at 0 mV) in a voltage-independent manner. This effect was seen in a CO(2)/HCO(3)(-)-, but not in a HEPES-buffered extracellular solution at pH 7.4 (n = 6). When the pH of a HEPES-buffered extracellular solution was lowered from 7.4 to 7. 0, hypoxia augmented the Ca(2+) current by 20 +/- 5% (n = 4, at 0 mV). Nisoldipine, an L-type Ca(2+) channel blocker (2 microM, n = 6), prevented, whereas, omega-conotoxin MVIIC (2 microM, n = 6), an inhibitor of N and P/Q type Ca(2+) channels, did not prevent augmentation of the Ca(2+) current by hypoxia, implying that low oxygen affects L-type Ca(2+) channels in glomus cells. Protein kinase C (PKC) inhibitors, staurosporine (100 nM, n = 6) and bisindolylmaleimide (2 microM, n = 8, at 0 mV), prevented, whereas, a protein kinase A inhibitor (4 nM PKAi, n = 10) did not prevent the hypoxia-induced increase of the Ca(2+) current. Phorbol 12-myristate 13-acetate (PMA, 100 nM), a PKC activator, augmented the Ca(2+) current by 20 +/- 3% (n = 8, at 0 mV). In glomus cells treated with PMA overnight (100 nM), hypoxia did not augment the Ca(2+) current (-3 + 4%, n = 5, at 0 mV). Immunocytochemical analysis revealed PKCdelta-like immunoreactivity in the cytosol of the glomus cells. Following hypoxia (6% O(2) for 5 min), PKCdelta-like immunoreactivity translocated to the plasma membrane in 87 +/- 3% of the cells, indicating PKC activation. These results demonstrate that hypoxia augments Ca(2+) current through L-type Ca(2+) channels via a PKC-sensitive mechanism.  (+info)

Pore block versus intrinsic gating in the mechanism of inward rectification in strongly rectifying IRK1 channels. (4/141)

The IRK1 channel is inhibited by intracellular cations such as Mg(2+) and polyamines in a voltage-dependent manner, which renders its I-V curve strongly inwardly rectifying. However, even in excised patches exhaustively perfused with a commonly used artificial intracellular solution nominally free of Mg(2+) and polyamines, the macroscopic I-V curve of the channels displays modest rectification. This observation forms the basis of a hypothesis, alternative to the pore-blocking hypothesis, that inward rectification reflects the enhancement of intrinsic channel gating by intracellular cations. We find, however, that residual rectification is caused primarily by the commonly used pH buffer HEPES and/or some accompanying impurity. Therefore, inward rectification in the strong rectifier IRK1, as in the weak rectifier ROMK1, can be accounted for by voltage-dependent block of its ion conduction pore by intracellular cations.  (+info)

Contributions of protein disulfide isomerase domains to its chaperone activity. (5/141)

Protein disulfide isomerase (PDI), a member of the thioredoxin (Trx) superfamily, consists of five consecutive domains, a-b-b'-a'-c. Domain combinations, AB, A'C, B'A'C and AB-C, and hybrids of PDI domains with Trx, Trx-C and Trx-B'A'C, have been constructed and expressed in Escherichia coli to examine the contributions of PDI domains to its enzyme and chaperone activities. All the combination and hybrid products are considerably less active than intact PDI in their enzyme activities. Recombinant products containing C, at low concentrations, inhibit the reactivation of lysozyme in HEPES buffer, while those without C do not. Only the intact PDI molecule and the hybrid molecule, Trx-B'A'C, but to a much lower level, show general chaperone activity in assisting the reactivation of denatured D-glyceraldehyde-3-phosphate dehydrogenase. It is suggested that all domains of PDI contribute to the binding of target protein for its chaperone activity.  (+info)

Mechanisms of endothelial cell swelling from lactacidosis studied in vitro. (6/141)

One of the early sequelae of ischemia is an increase of circulating lactic acid that occurs in response to anaerobic metabolism. The purpose of the present study was to investigate whether lactic acidosis can induce endothelial swelling in vitro under closely controlled extracellular conditions. Cell volume of suspended cultured bovine aortic endothelial cells was measured by use of an advanced Coulter technique employing the "pulse area analysis" signal-processing technique (CASY1). The isosmotic reduction of pH from 7.4 to 6.8 had no effect on cell volume. Lowering of pH to 6.6, 6.4, or 6.0, however, led to significant, pH-dependent increases of cell volume. Swelling was more pronounced in bicarbonate-buffered media than in HEPES buffer. Specific inhibition of Na(+)/H(+) exchange by ethylisopropylamiloride completely prevented swelling in HEPES-buffered media. Pretreatment with ouabain to partially depolarize the cells did not affect the degree of acidosis-induced swelling. In bicarbonate-buffered media, the inhibition of transmembrane HCO(3)(-) transport by DIDS reduced swelling to a level comparable with that seen in the absence of bicarbonate ions. Lactacidosis-induced endothelial swelling, therefore, is a result of intracellular pH regulatory mechanisms, namely, Na(+)/H(+) exchange and bicarbonate-transporting carriers.  (+info)

Regulation of the epithelial Na(+) channel by extracellular acidification. (7/141)

The effect of extracellular acidification was tested on the native epithelial Na(+) channel (ENaC) in A6 epithelia and on the cloned ENaC expressed in Xenopus oocytes. Channel activity was determined utilizing blocker-induced fluctuation analysis in A6 epithelia and dual electrode voltage clamp in oocytes. In A6 cells, a decrease of extracellular pH (pH(o)) from 7.4 to 6.4 caused a slow stimulation of the amiloride-sensitive short-circuit current (I(Na)) by 68.4 +/- 11% (n = 9) at 60 min. This increase of I(Na) was attributed to an increase of open channel and total channel (N(T)) densities. Similar changes were observed with pH(o) 5.4. The effects of pH(o) were blocked by buffering intracellular Ca(2+) with 5 microM 1, 2-bis(2-aminophenoxy)ethane-N,N,N',N'-tetraacetic acid. In oocytes, pH(o) 6.4 elicited a small transient increase of the slope conductance of the cloned ENaC (11.4 +/- 2.2% at 2 min) followed by a decrease to 83.7 +/- 11.7% of control at 60 min (n = 6). Thus small decreases of pH(o) stimulate the native ENaC by increasing N(T) but do not appreciably affect ENaC expressed in Xenopus oocytes. These effects are distinct from those observed with decreasing intracellular pH with permeant buffers that are known to inhibit ENaC.  (+info)

Binding of dystrophin's tandem calponin homology domain to F-actin is modulated by actin's structure. (8/141)

Dystrophin has been shown to be associated in cells with actin bundles. Dys-246, an N-terminal recombinant protein encoding the first 246 residues of dystrophin, includes two calponin-homology (CH) domains, and is similar to a large class of F-actin cross-linking proteins including alpha-actinin, fimbrin, and spectrin. It has been shown that expression or microinjection of amino-terminal fragments of dystrophin or the closely related utrophin resulted in the localization of these protein domains to actin bundles. However, in vitro studies have failed to detect any bundling of actin by either intact dystrophin or Dys-246. We show here that the structure of F-actin can be modulated so that there are two modes of Dys-246 binding, from bundling actin filaments to only binding to single filaments. The changes in F-actin structure that allow Dys-246 to bundle filaments are induced by covalent modification of Cys-374, proteolytic cleavage of F-actin's C-terminus, mutation of yeast actin's N-terminus, and different buffers. The present results suggest that F-actin's structural state can have a large influence on the nature of actin's interaction with other proteins, and these different states need to be considered when conducting in vitro assays.  (+info)

HEPES, Free Acid | CAS 7365-45-9 | Goods Buffer | P212121 StoreHEPES, Free Acid | CAS 7365-45-9 | Good's Buffer | P212121 Store
HEPES is a zwitterionic organic chemical buffering agent. HEPES is commonly added to media at concentrations ranging from 10 mM to 25 mM. Concentrations greater than 50 mM are not recommended since it can result in cell toxicity. Depending on your application the use of HEPES with Cu(II) should be carefully considered as a possible interaction may occur1.. Recently, HEPES has found an increasing role outside the area of biochemisty such as in the field of nanoparticles2,3,4,5.. Buffering pH range 6.8 - 8.2.. Free Shipping within the Continental USA. ...
Establishment of a standardized post-embedding method for immunoelectron microscopy by applying heat-induced antigen retrievalEstablishment of a standardized post-embedding method for immunoelectron microscopy by applying heat-induced antigen retrieval
Abstract: We have developed a new standardized method for the post-embedding immunoelectron microscopy using the same fixation, antigen retrieval and image contrasting procedures. Tissues were fixed with 4% formaldehyde containing 2.5 mM CaCl2, 1.25 mM MgCl2 in a 0.1 M 4-(2-hydroxyethyl)-piperazineethanesulfonic acid (HEPES) buffer (pH 7.4) for 2 h and then with the same fixative composition in 0.1 M HEPES buffer (pH 8.5) overnight at room temperature. Vehicle osmolarity of fixatives was adjusted to 300-330 mOsm by adding glucose. The specimens were dehydrated with dimethylformamide on ice and embedded in LR-White resin. Ultrathin sections were heated in a 20 mM Tris-HCl buffer (pH 9.0) for 1-2 h at 95°C. After immuno-gold labeling, the sections were treated with 2% glutaraldehyde containing 0.05% tannic acid in a 0.1 M phosphate buffer (pH 5.5) for 5 min and with a 1% OsO4/0.1 M phosphate buffer (pH 7.4) for 5 min, and then they were double stained with uranyl acetate and lead citrate. The ...
HEPES (Fine White Crystals/Molecular Biology), Fisher BioReagents Poly Bottle; 500g HEPES (Fine White Crystals/Molecular...HEPES (Fine White Crystals/Molecular Biology), Fisher BioReagents Poly Bottle; 500g HEPES (Fine White Crystals/Molecular...
HEPES (Fine White Crystals/Molecular Biology), Fisher BioReagents Poly Bottle; 500g HEPES (Fine White Crystals/Molecular Biology), Fisher BioReagents Buffering Agents
Gentaur Molecular :Biosera \ DMEM F12 w L Glutamine w o Sodium Bicarbonate w 15 mM Hepes For 5L \ PM-D1227/5Gentaur Molecular :Biosera \ DMEM F12 w L Glutamine w o Sodium Bicarbonate w 15 mM Hepes For 5L \ PM-D1227/5
Gentaur molecular products has all kinds of products like :search , Biosera \ DMEM _ F12 w_ L_Glutamine w_o Sodium Bicarbonate w_ 15 mM Hepes _ For 5L \ PM-D1227/5 for more molecular products just contact us
Gentaur Molecular :Biosera \ MEM w Earles Salts w L Glutamine w o Sodium Bicarbonate w 25mM Hepes For 10L \...Gentaur Molecular :Biosera \ MEM w Earle's Salts w L Glutamine w o Sodium Bicarbonate w 25mM Hepes For 10L \...
Gentaur molecular products has all kinds of products like :search , Biosera \ MEM w_ Earles Salts w_ L_Glutamine w_o Sodium Bicarbonate w_ 25mM Hepes _ For 10L \ PM-E1158/10 for more molecular products just contact us
Trapp Stimulates Guanine Nucleotide Exchange on Ypt1p | Journal of Cell Biology | Rockefeller University PressTrapp Stimulates Guanine Nucleotide Exchange on Ypt1p | Journal of Cell Biology | Rockefeller University Press
TRAPP was purified from 300 OD599 units of cells. SFNY904 (MATα ura3-52 bet3Δ::URA3 leu2-3, 112 BET3-protein A::LEU2 L-A-o) was used for the purification because it contained protein A-tagged Bet3p and was free of the L-A virus (L-A-o). The L-A virus coat protein, gag, is a common contaminant in the purification (see Sacher et al. 2000). To purify TRAPP, cells were converted to spheroplasts, lysed in 3 ml of buffer B (150 mM KCl, 20 mM Hepes, pH 7.2, 2 mM EDTA, 1% Trition X-100, 0.5 mM DTT, protease inhibitor cocktail), and the unbroken cells were removed as described above. The lysate was centrifuged at 14,000 g for 10 min and the supernatant (10 mg/ml of protein) was incubated with 150 μl of a 50% slurry of IgG-Sepharose (Amersham Pharmacia Biotech) for 4 h. The beads were washed three times with 3 ml of release buffer (20 mM Hepes, pH 7.2, 5 mM MgCl2, 1 mM DTT, 0.75 mM GTP, 0.75 mMGDP, 1 mg/ml BSA) or uptake buffer (20 mM Hepes, pH 7.2, 5 mM MgCl2, 1 mM EDTA, 1 mM ATP, 1 mM DTT, 1 mg/ml ...
Neuromics: April 2015Neuromics: April 2015
Expansion of BMSCs: Bone marrow aspirate collections containing 8 x 107 MNCs were seeded within each 150-cm2 tissue culture flask. Culture medium composed of alpha-minimal essential medium (α-MEM) supplemented with 10% v/v heat-inactivated fetal bovine serum (FBS), penicillinstreptomycin-glutamine, 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES), and sodium pyruvate (all from Life Technologies, Burlington, Canada) was pipetted into each flask. Fibroblast growth factor-2 (FGF-2; Neuromics Inc., Edina, USA) was added at a concentration of 5 ng/ml in order to maintain cell multipotency. Nucleated cells were allowed to adhere and grow for seven days before the first media change under normoxia (ambient 21% O2) or hypoxia (low 3% O2) at 37°C in a humidified incubator containing 5% CO2. Flasks from the hypoxic incubator experienced short periods (less than 5 minutes) of normoxic exposure during media changes. Thereafter, the media were changed twice per week until 80% cell confluence was ...
Plus itPlus it
Background: Some respiratory diseases may induce alveolar hypoxia thereby hypoxic pulmonary vasoconstriction (HPV). This study was the first to investigate the role of anion exchanger in sustained HPV.. Methods: Experiments were performed in the isolated perfused rabbit lung. In the HCO3- free groups, HEPES were added to the perfusate instead of bicarbonate. In the HEPES1 and HEPES2 groups, the lungs were ventilated with hypoxic gas with or without CO2 respectively.. Results: Ventilation the lungs with hypoxic gas resulted in biphasic HPV. No alteration in both phases of HPV was detected by DIDS (anion exchanger inhibitor,200 microM). However, DIDS (400 microM), extended ascending part of acute HPV until min 24. Both phases of HPV were decreased in the HEPES1 group. But in the HEPES 2 group, HPV tended to increase significantly during rising part of acute phase with no change during sustained phase.. Conclusions: This study is suggesting that anion exchanger may modulate HPV especially during ...
Plus itPlus it
Growth of the mouse mammary epithelial cell line designated COMMA-D has been studied in serum-free medium (SFM) formulated with Hams F12 and Dulbeccos modified Eagles medium (1/1) containing 15 mm 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid, 2 mm glutamine, gentamicin (50 µg/ml; basal medium) and supplemented with insulin (10 µg/ml), transferrin (10 µg/ml), selenous acid (10 ng/ml), epidermal growth factor (20 ng/ml; EGF), 10 nm 3,5,3′-triiodothyronine, 50 µm ethanolamine, 1.0 nm 17β-estradiol, 65 µm glutathione, and ovalbumin (100 µg/ml). COMMA-D cells were able to undergo serial passage and continued to exhibit dome formation after 20 passages in SFM. Cells seeded at low density in SFM underwent four population doublings at low passage number in 1 week compared to six doublings for cells grown in medium containing insulin, transferrin, selenium, EGF, and 1% fetal bovine serum. After many passages in SFM, the growth rates of cells were similar to those in serum-supplemented ...
Generation of intracellular pH gradients in single cardiac myocytes with a microperfusion system. - Oxford NeuroscienceGeneration of intracellular pH gradients in single cardiac myocytes with a microperfusion system. - Oxford Neuroscience
This study describes the use of a microperfusion system to create rapid, large regional changes in intracellular pH (pH(i)) within single ventricular myocytes. The spatial distribution of pH(i) in single myocytes was measured with seminaphthorhodafluor-1 fluorescence using confocal imaging. Changes in pH(i) were induced by local external application of NH(4)Cl, CO(2), or sodium propionate. Local application was achieved by simultaneously directing two parallel square microstreams, each 275 microm wide, over a single myocyte oriented perpendicular to the direction of flow. One stream contained the control solution, and the other contained a weak acid or base. End-to-end, stable pH(i) gradients as large as 1 pH unit were readily created with this technique. This result indicates that pH within a single cardiac cell may not always be spatially uniform, particularly when weak acid or base gradients are present, which can occur, for example, in regional myocardial ischemia. The microperfusion method should
An East Asian Common Variant Vinculin P.Asp841His Was Associated With Sudden Unexplained Nocturnal Death Syndrome in the...An East Asian Common Variant Vinculin P.Asp841His Was Associated With Sudden Unexplained Nocturnal Death Syndrome in the...
Macroscopic voltage‐gated sodium current (INa) was measured 24 hours after transfection with the standard whole‐cell patch clamp method at 22°C in both HEK293 cells and iPS‐CMs under both normal (pH 7.4) and moderate acidosis (extracellular and intracellular pH 7.0) conditions. For HEK293 cells, the pipette solution contained (in mmol/L) EGTA 2, CsF 120, CsCl2 20, HEPES 5, and NaCl 5 and was adjusted to pH 7.4 or 7.0 with CsOH; the bath solution contained (in mmol/L) CaCl2 1.8, NaCl 140, HEPES 5, MgCl2 0.75, and KCl 4 and was adjusted to pH 7.4 or 7.0 with NaOH.20, 21 For iPS‐CMs, the pipette solution contained (in mmol/L) HEPES 10, NaCl 5, CaCl2 2, CsCl2 135, MgATP 5, and EGTA 10 and was adjusted to pH 7.4 or 7.0 with CsOH; the bath solution contained (in mmol/L) CsCl2 105, NaCl 60, glucose 10, CaCl2 1.8, MgCl2 1, HEPES 5, and Nifedipine 0.001 and was adjusted to pH 7.4 or 7.0 with CsOH (modified from reference).22 The resistances of microelectrodes ranged from 1.0 to 2.3 MΩ. Voltage ...
International Journal of Pharmaceutics (v.378, #1-2) | www.chemweb.comInternational Journal of Pharmaceutics (v.378, #1-2) | www.chemweb.com
This study discusses the effect of key factors like containers, buffers and the freeze (controlled vs. flash freezing) and thawing processes on the stability of a therapeutic protein fibroblast growth factor 20 (FGF-20). The freezing profiles monitored by 15 temperature probes located at different regions in a 2-L bottle during freezing can be grouped into three categories. A rapid drop in temperature was observed at the bottom followed by the top and middle center of the bottle. The freeze-thawing behavior in a 50 ml tube is considerably uniform, as expected. Among phosphate, HEPES (4-(2-hydroxyethyl)-1-piperazine ethanesulfonic acid), citrate and histidine (each containing 0.5 M arginine-sulfate) buffer systems, a minimum pH change (0.4 pH unit vs. ∼1.7 pH unit) was observed for the phosphate buffer system. Thawing in a 50 ml tube at room temperature standing resulted in a significant phase separation in citrate, histidine and HEPES buffers; however, phase separation was least in the ...
He catalytic activity; replacement of HEPES/KOH buffer with TRIS/HCl | CTSK Inhibito ctskinhibito.comHe catalytic activity; replacement of HEPES/KOH buffer with TRIS/HCl | CTSK Inhibito ctskinhibito.com
He catalytic activity; replacement of HEPES/KOH buffer with TRIS/HCl abolished the enzymatic activity unless a monovalent cation was present (Figure 3C). Among
Iscoves Modification of DMEM - Iscoves Modification of DMEM - Classical Media - ProductsIscove's Modification of DMEM - Iscove's Modification of DMEM - Classical Media - Products
|p||strong|Technical Advantage: |/strong| Lower documented Endotoxin content then offerings from other major suppliers|br /| Iscoves Modification of DMEM (IMDM) is highly enriched synthetic medium optimized for rapidly proliferating cultures of high cell density.  A modified form of Dulbeccos Modified Eagle Medium (DMEM), Iscoves contains additional amino acids, vitamins, selenium and HEPES buffer, and is formulated with potassium nitrate in place of ferric nitrate. The addition of albumin and transferrin may be required for certain cell types.  This formulation contains 25 mM HEPES, and does not contain L-glutamine, alpha-thioglycerol and beta-mercaptoethanol.|br /| -  Complete Certificate of Analysis available for each production lot, along with full formulation information|br /| -  Produced under the highest industry standards to ensure superior results|/p|
Ecdysone-induced expression of the caspase DRONC during hormone-dependent programmed cell death in Drosophila is regulated by...Ecdysone-induced expression of the caspase DRONC during hormone-dependent programmed cell death in Drosophila is regulated by...
7.5 × 106 l(2)mbn cells were pelleted, washed once in PBS, and resuspended in 800 μl of buffer A (10 mM Hepes, pH 7.6, 10 mM KCl, 1.5 mM MgCl2, 0.1 mM EDTA, 0.1 mM EGTA, Complete™ protease inhibitors; Roche), and placed on ice for 15 min. NP40 was added to 0.1%, vortexed for 30 s, and centrifuged at 13 K for 30 s at 4°C. Nuclear pellet was resuspended in 80 μl buffer C (10mM Hepes, pH 7.6, 400 mM NaCl, 7.5 mM MgCl2, 0.2 mM EDTA, 0.1 mM EGTA, 1 mM DTT, 0.5 mM PMSF, Complete™), and incubated on ice for 40 min while shaking. Extract was then centrifuged for 5 min at 13 K and supernatant aliquoted and frozen at -70° C. Nuclear extracts from second instar, wandering third instar, early pupae, and larvae specifically staged at -18 and -4 h (relative to puparium formation) from wild-type and rbp5 larvae were prepared as follows. Extracts were made by homogenizing 50 larvae in 300 μl of buffer A and removing them to a fresh tube in a total of 600 μl devoid of larval debri. After incubation on ...
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To determine selectivity against αIIbβIIIa, wells of a 96-well Immulon-2 microtiter plate were coated with 10 μg/ml RGD affinity-purified human αIIbβIIIa in 10 mM HEPES, 150 mM NaCl, and 1 mM MgCl2, pH 7.4 (500 ng/well αIIbβIIIa final), and then the pate was incubated overnight at 4°C. The next day, the wells were blocked with 5% BSA in the above-mentioned buffer at room temperature for 2 h. The assay plate was washed five times with modified Tyrodes buffer (150 mM NaCl, 12 mM NaHCO3, 2.6 mM KCl, 2.5 mM HEPES, 1 mM MgCl2, and 1 mg/ml BSA, pH 7.4). Biotinylated fibrinogen was prepared using human fibrinogen according to directions from the biotin-X-NHS biotinylation kit. Fifty microliters of the compound/biotinylated fibrinogen mix was transferred to the assay plate and incubated at room temperature for 2 h. After incubation, the assay plate was washed five times with modified Tyrodes buffer. Reactions were visualized as described above. JNJ-26076713 was tested at half-log doses from ...
P0192-N1L - IMDM w/ L-Glutamine w/ 25 mM Hepes w/o Phenol Red - Jain BiologicalsP0192-N1L - IMDM w/ L-Glutamine w/ 25 mM Hepes w/o Phenol Red - Jain Biologicals
IMDM - ISCOVES MODIFIED DULBECCOS MEDIUM. In 1976, Guilbert and Iscove demonstrated that precursor cells of erythrocytes and macrophages could be cultured in a reduced-serum medium supplemented with albumin, transferrin, lecithin, and selenium.. Iscoves Medium is a variation of Dulbeccos Modified Eagles Medium (DMEM) containing:. ...
pHi Regulation in Myocardium of the Spontaneously Hypertensive Rat | Circulation ResearchpHi Regulation in Myocardium of the Spontaneously Hypertensive Rat | Circulation Research
The increased steady state myocardial pHi in the SHR in nominally bicarbonate-free buffer provides evidence for hyperactivity of the Na+-H+ exchanger in the hypertensive and/or hypertrophic myocardium. This evidence was further strengthened by the following findings: (1) the faster recovery from the intracellular acid load induced by switching from HEPES to CO2/HCO3− buffer and the suppression of this difference when the Na+-H+ exchanger was inhibited, (2) the greater effect of blocking the Na+-H+ exchanger with EIPA in SHR myocardium exposed to HEPES-buffer, and (3) the lack of effect of the disulfonic derivative SITS on resting pHi in SHR myocardium in HEPES buffer bubbled with oxygen, ruling out a possible role of alkalinizing bicarbonate-dependent mechanisms.32 A still unanswered question in the present study is whether the hyperactivity of the Na+-H+ exchanger that we detected is a characteristic of the hypertensive animals or of the hypertrophic myocardium itself. Further experiments are ...
0.006404</PDBx:fract transf...0.006404</PDBx:fract transf...
0.1M ammonium sulfate, 24-32% PEG5000 monomethyl ether, 1mM ATP, 100mM Hepes buffer, pH 7.2, VAPOR DIFFUSION, SITTING DROP, temperature ...
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The vascular effect of peroxynitrite (ONOO-), a product of superoxide anion and nitric oxide, in isolated canine coronary arteries bathed in a 10 mM 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid-buffered physiological salt solution (pH 7.4) was investigated. ONOO- was synthesized from nitrite and H2O2 in a quenched-flow reactor. Addition of 0.01 to 30 microM ONOO- produced a rapid, dose-dependent relaxation in all 17 rings of coronary arteries with a threshold concentration of 0.1 microM, IC50 of 1.0 +/- 0.1 microM and Emax of -96 +/- 0.5% (Means +/- S.E.M.). Incubation of arteries in a standard bicarbonate-buffered Krebs-Henseleit solution decreased slightly the sensitivity of ONOO- relaxation but did not alter the maximum effect (Emax = -97 +/- 1.1, n = 6 vessels). The ONOO(-)-induced coronary relaxation was reversible upon washing, and was also reproducible with repeated testings in the same ring. Mechanical removal of intimal endothelium did not alter the observed relaxant effect. ...
Iscoves Modified Dulbeccos Medium (IMDM) - Caisson Laboratories, Inc.Iscove's Modified Dulbecco's Medium (IMDM) - Caisson Laboratories, Inc.
With HEPES buffer, L-glutamine, and phenol red. Without sodium bicarbonate. Store at 2˚ to 8˚C.. Find this and many more products.
A Turn-Off Red-emitting fluorophore for nanomolar detection of HeparinA "Turn-Off" Red-emitting fluorophore for nanomolar detection of Heparin
A simple fluorophore bearing a diethylaminocoumarin donor and a pyridinium acceptor was synthesized and utilized for the ultra-sensitive detection of heparin. The synthesized dicationic push-pull coumarin derivative emits strongly in the red-region (665 nm) and detects nanomolar concentrations (14.8 nM to 148 nM) of heparin in HEPES buffer and FBS serum solutions. The dication exhibits excellent fluorescence selectivity and sensitivity towards heparin over its analogues such as chondroitin 4-sulfate (CS), hyaluronic acid (HA) and dextran. This fluorescence assay is a convenient, sensitive method for monitoring heparin levels in biological samples. These findings were confirmed using coarse-grained Monte Carlo simulations, which provide us with a rationale for the selective binding of heparin ...
Human Retina-RPE-Choroidea Perfusion Tissue Culture: An in vitro Model to Investigate Retinal Pigmentepitheliopathies in Man |...Human Retina-RPE-Choroidea Perfusion Tissue Culture: An in vitro Model to Investigate Retinal Pigmentepitheliopathies in Man |...
Purpose: : The human RPE is a polar organized, highly specialized epithelium. To maintain its functions multiple interactions among the RPE-cells and the neighboring tissues are necessary and play an important role in the pathology of innummerous diseases of the posterior segment. This study aims to investigate whether a perfusion tissue culture of the human retina-RPE-choroidea complex could be a suitable model to study these diseases in vitro. Methods: : Human retina-RPE-choroidea-tissue was isolated from patients after enucleation because of various reasons. Written consent was obtained prior to surgery. After removal of the anterior segment and the vitreous the choroidea-RPE-retina complex was separated from the sclera and transferred immediately into a gradient tissue culture chamber (Minucells&Minutissue, Bad Abbach, Germany) kept at 37°C and perfused with DMEM and Hams F12 1:1, supplemented with 10% human serum, 25mM HEPES and 1% penicillin-streptomycin, 110mg/l sodium-pyruvat for five ...
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After 1 d in culture, cells were continuously superfused (2 ml/min) with physiological solution containing the following (in mm): 152 NaCl, 5 KCl, 1 MgCl2, 2 CaCl2, 10 glucose, and 10 HEPES, pH adjusted to 7.4 with NaOH, as previously reported (Fabbretti et al., 2006). Cells were voltage clamped (whole-cell configuration) using pipettes filled with the following (in mm): 140 KCl, 0.5 CaCl2, 2 MgCl2, 2 Mg2ATP3, 2 GTP, 10 HEPES, and 10 EGTA, pH adjusted to 7.2 with KOH (at −60 mV, 70% series resistance compensation). Agonists were applied with a fast superfusion system (Rapid Solution Changer RSC-200; Bio-Logic, Claix, France) with solution exchange time (10-90%) of 30-40 ms. Responses to agonists were measured in terms of peak amplitude. To express agonist potency in terms of EC50 values (concentration producing 50% of the maximum response), dose-response curves for the selective P2X3 receptor agonist α,β-methyleneATP (α,β-meATP) were constructed by applying different agonist doses to the ...
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Electrophysiology. Whole-cell recordings from acutely isolated rat striatal neurons were obtained using previously published techniques (Surmeier et al., 1995; Mermelstein et al., 1999). The pipette solution consisted of (in mm): 180N-methyl-d-glucamine (NMG), 40 HEPES, 4 MgCl2, 0-20 BAPTA, 12 phosphocreatine, 2 Na2ATP, 0.2 Na2GTP, and 0.1 leupeptin, pH 7.2-7.3 with H2SO4, 265-270 mOsm/l. The external solution consisted of (in mm): 135 NaCl, 20 CsCl, 1 MgCl2, 10 HEPES, 0.001 TTX, 5 BaCl2, and 10 glucose, pH 7.4 with NaOH, 300-305 mOsm/l. All reagents were obtained from Sigma (St. Louis, MO) except ATP and GTP (Boehringer Mannheim, Indianapolis, IN) and BAPTA, calcineurin autoinhibitory peptide, and leupeptin (Calbiochem, La Jolla, CA). All drugs were prepared according to the manufacturers specifications and applied with a "sewer pipe" capillary array (Surmeier et al., 1995; Mermelstein et al., 1999). C terminus of β adrenergic receptor kinase 1 (βARK-C) peptide (βARK-Cp) is comprised of ...
DMEM, high glucose, HEPES, no phenol redDMEM, high glucose, HEPES, no phenol red
DMEM (Dulbeccos Modified Eagle Medium) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM include primary fibroblasts, neurons, glial cells, HUVECs, and smooth muscle cells, as well as cell lines such as HeLa, 293, Cos-7, a
bmse000792 HEPES - Supplemental Proton Data at BMRBbmse000792 HEPES - Supplemental Proton Data at BMRB
InCHi String: isomeric and canonical SMILES: C1CN(CC[NH+]1CCO)CCS(=O)(=O)[O-]. IUPAC: IUPAC traditional: IUPAC cas: IUPAC openeye: IUPAC systematic ...
HEPES, Free Acid, High Purity | BioExpressHEPES, Free Acid, High Purity | BioExpress
BioExpress accepts no responsibility and shall not be held liable for loss, damage, expense, consequential, or accidental damage. This includes the damage to property, person, or premises resulting from these products. BioExpress MAKES NO WARRANTIES, EXPRESSED OR IMPLIED, INCLUDING, WITHOUT LIMITATION, THE WARRANTIES OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURCHASE. BioExpress expressly disclaims all other warranties, whether express, implied or statutory, including the warranty of MERCHANTABILITY AND FITNESS FOR USE. In no event will BioExpress be liable for consequential damages arising from the use of products described in this catalog.. ...
HEPES Sodium Salt Specification Comparison | China-Mainland | Sigma-AldrichHEPES Sodium Salt Specification Comparison | China-Mainland | Sigma-Aldrich
2018 Merck KGaA, Darmstadt, Germany and/or its affiliates. 保留所有权利。严格禁止在未经同意的情况下转载本网站之所有信息。 Sigma-Aldrich品牌产品均由Sigma-Aldrich LLC.独家贩售。的注册商标。沪ICP备14038167号-2. 使用条款 , 隐私权原则 ...
SuperCult® RPMI 1640 without HEPES and L-glutamine | Creative BioarraySuperCult® RPMI 1640 without HEPES and L-glutamine | Creative Bioarray
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the Pub Med ID of your paper to get a coupon. ...
Entecavir | HIV Infection & Hepes Treatment | Online PharmacyEntecavir | HIV Infection & Hepes Treatment | Online Pharmacy
Entecavir is used in the treatment of HIV Infection and Herpes Infection. BUY the tablets from Cheap Medicine Shop and save more on all your medicines.
Hampton ResearchHampton Research
Synonyms: HEPES sodium salt, or 4-(2-hydroxyethyl)piperazine-1-ethanesulfonic acid sodium salt, or N-(2-hydroxyethyl)piperazine-N-(2-ethanesulfonic acid) sodium salt ...
Tubastatin A | ≥99%(HPLC) | Selleck | HDAC inhibitor ...Tubastatin A | ≥99%(HPLC) | Selleck | HDAC inhibitor ...
HDAC enzymatic assays:. Tubastatin A is dissolved and diluted in assay buffer (50 mM HEPES, pH 7.4, 100 mM KCl, 0.001% Tween-20, 0.05% BSA, and 20 μM tris(2-carboxyethyl)phosphine) to 6-fold of the final concentration. HDAC enzymes are diluted to 1.5-fold of the final concentration in assay buffer and pre-incubated with Tubastatin A for 10 minutes before the addition of the substrate. The amount of FTS (HDAC1, HDAC2, HDAC3, and HDAC6) or MAZ-1675 (HDAC4, HDAC5, HDAC7, HDAC8, and HDAC9) used for each enzyme is equal to the Michaelis constant (Km), as determined by a titration curve. FTS or MAZ-1675 is diluted in assay buffer to 6-fold the final concentration with 0.3 μM sequencing grade trypsin. The substrate/trypsin mix is added to the enzyme/compound mix and the plate is shaken for 60 seconds and then placed into a SpectraMax M5 microtiter plate reader. The enzymatic reaction is monitored for release of 7-amino-4-methoxy-coumarin over 30 minutes, after deacetylation of the lysine side chain ...
PENN SCAP-T Protocols | SCAP-TPENN SCAP-T Protocols | SCAP-T
CM cell collection Version 3 University of Pennsylvania For Patients 14-17 Human cardiomyocyte dissociation. Myocardial tissue was washed and resuspended in cold isolation buffer (130 mM NaCl; 5 mM KCl; 1.2 mM KH2PO4; 6 mM HEPES; 5 mM NaHCO3; 1 mM MgCl2; 5 mM Glucose) pH 7.4. Isolation buffer was supplemented with 0.36mM CaCl2 for enzyme activity. Cardiac tissue was incubated for 15 -20mins in isolation buffer supplemented with Collagenase IV (5mg/ml stock ,Sigma). After each incubation step, the supernatants were transferred to a tube and centrifuged at 600 rpm for 4 min. Cell pellets were resuspended in ice-cold isolation buffer. Several rounds of digestion were performed until tissue was fully digested.. Flow Cytometry and FACS. Isolated cardiac cell suspensions obtained from human heart tissue were sorted on a FACSAria (Becton Dickenson Biosciences) instrument operating at low pressure (20 psi) using a 100µm nozzle. Initial size gates for forward scatter (FSC) vs. side scatter (SSC) were ...
PENN SCAP-T Protocols | SCAP-TPENN SCAP-T Protocols | SCAP-T
CM cell collection Version 4 University of Pennsylvania For Patients 18-19 Date: 12/2014 Human cardiomyocyte dissociation. Myocardial tissue was washed and resuspended in cold isolation buffer (130 mM NaCl; 5 mM KCl; 1.2 mM KH2PO4; 6 mM HEPES; 5 mM NaHCO3; 1 mM MgCl2; 5 mM Glucose) pH 7.4. Isolation buffer was supplemented with 0.36mM CaCl2 for enzyme activity. Cardiac tissue was incubated for 15 -20mins in isolation buffer supplemented with Collagenase IV (5mg/ml stock ,Sigma). After each incubation step, the supernatants were transferred to a tube and centrifuged at 600 rpm for 4 min. Cell pellets were resuspended in ice-cold isolation buffer. Several rounds of digestion were performed until tissue was fully digested.. Flow Cytometry and FACS.. Isolated cardiac cell suspensions obtained from human heart tissue were sorted on a FACSAria (Becton Dickenson Biosciences) instrument operating at low pressure (20 psi) using a 100µm nozzle. Initial size gates for forward scatter (FSC) vs. side ...
JCI Insight - LipidFinder: A computational workflow for discovery of lipids identifies eicosanoid-phosphoinositides in plateletsJCI Insight - LipidFinder: A computational workflow for discovery of lipids identifies eicosanoid-phosphoinositides in platelets
Human platelet isolation. Blood was collected from three genetically unrelated donors that were free from nonsteroidal antiinflammatory drugs for at least 14 days. Blood was collected into acid-citrate-dextrose (ACD; 85 mM trisodium citrate, 65 mM citric acid, and 100 mM glucose) at a blood/ACD ratio of 8.1:1.9 (v/v) and centrifuged at 250 g for 10 minutes at room temperature (22°C). Platelet-rich plasma was collected and centrifuged at 900 g for 10 minutes, and the pellet resuspended in Tyrodes buffer (134 mM NaCl, 12 mM NaHCO3, 2.9 mM KCl, 0.34 mM Na2HPO4, 1.0 mM MgCl2, 10 mM HEPES, and 5 mM glucose, pH 7.4) containing ACD (9:1, v/v). Platelets were washed by centrifuging at 800 g for 10 minutes and then resuspended in Tyrodes buffer at a concentration of 2 × 108 cells/ml. Where activated, they were prewarmed for 1 min at 37°C, with 1 mM CaCl2, before addition of 0.2 U/ml thrombin for 30 min.. Lipid extraction. Lipids were extracted by adding a solvent mixture (1 M acetic ...
Dulbeccos Modified Eagles/Hams F-12 Medium 1:1 (DMEF) - Caisson Laboratories, Inc.Dulbecco's Modified Eagle's/Ham's F-12 Medium 1:1 (DMEF) - Caisson Laboratories, Inc.
With L-glutamine, sodium pyruvate, and 15 mM HEPES. Without phenol red and sodium bicarbonate. Store at 2˚ to 8˚C.. Find this and many more products.
Roles of Amino Acids and Subunits in Determining the Inhibition of Nicotinic Acetylcholine Receptors by Competitive Antagonists...Roles of Amino Acids and Subunits in Determining the Inhibition of Nicotinic Acetylcholine Receptors by Competitive Antagonists...
Patch pipettes, filled with a solution consisting of 140 mm KCl, 5 mm EGTA, 5 mm MgCl2, and 10 mm HEPES, pH 7.3, had resistances of 3-6 MΩ. An outside-out patch24 with a seal resistance of 5 GΩ or greater was excised from a cell and moved into position at the outflow of a HSSE-2 rapid perfusion device (ALA Scientific Instruments, Westbury, NY). The perfusion system consisted of solution reservoirs, manual switching valves, a solenoid-driven pinch valve, and two tubes inserted into the culture dish and had a time resolution of less than 100 μs.25 One tube contained extracellular solution without agonist (normal solution); the other contained extracellular solution with 300 μm acetylcholine (test solution). In the control protocol, the patch, initially perfused with normal solution, was exposed to a series of ten 0.25-s exposures to the test solution at 5-s intervals. Manual valves were used to connect to reservoirs containing a defined concentration of competitive antagonist with or without ...
JCI - A coagulation defect arising from heterozygous premature termination of tissue factorJCI - A coagulation defect arising from heterozygous premature termination of tissue factor
Cell culture. HUVECs (Lonza) were maintained for up to 5 passages in complete EGM-2 Endothelial Cell Growth Medium (Lonza) on gelatin-coated culture flasks. HEK293T cells (ATCC) were cultured in DMEM (Life Technologies) supplemented with 10% fetal bovine serum (HyClone). Cells were passaged using trypsin EDTA (0.05%, Thermo Fisher Scientific). Cells were cultured at 37°C and 5% carbon dioxide. iPSC methods are detailed below (see "iPSC culture" and "Differentiation of iPSCs").. Factor Xa and thrombin generation assays. Equal cell numbers were seeded in 96-well (20,000 HUVECs, 2500 differentiated VSMCs) or 384-well (3000 differentiated ECs) plate format and cultured in the appropriate medium (see "Cell culture"). Where indicated, endothelial cells were stimulated with tumor necrosis factor-α (TNF-α; 10 ng/mL; Calbiochem) for 3.5 hours at 37°C. Cells were washed with HBS-BSA (20 mM HEPES, pH 7.4, 150 mM NaCl, 5 mM KCl, 5 mM CaCl2 1 mg/mL bovine serum albumin) that was then replaced with ...
PF-573228 | FAK inhibitor | Read Reviews & Product Use CitationsPF-573228 | FAK inhibitor | Read Reviews & Product Use Citations
Affinity determination:. Purified activated FAK kinase domain (amino acids 410-689) is reacted with 50 μM ATP, and 10 μg/well of a random peptide polymer of Glu and Tyr (molar ratio of 4:1), poly(Glu/Tyr) in kinase buffer (50 mM HEPES, pH 7.5, 125 mM NaCl, 48 mM MgCl2) for 15 min. Phosphorylation of poly(Glu/Tyr) is challenged with serially diluted compounds at 1/2-Log concentrations starting at a top concentration of 1 μM. Each concentration is run in triplicate. Phosphorylation of poly(Glu/Tyr) is detected with a general anti-phospho-tyrosine (PY20) antibody, followed by horseradish peroxidase-conjugated goat anti-mouse IgG antibody. The standard horseradish peroxidase substrate 3, 3 ...
January | 2013 | Thrombin InhibitorsJanuary | 2013 | Thrombin Inhibitors
H1299, A549, MDA MB 134, MDA MB231, HEL, KG 1a, Mo91, Molm14, and K562 cells had been cultured in RPMI 1640 medium with 10% fetal how to dissolve peptide bovine serum. 293T and GP2 293 cells have been cultured in Dulbeccos modified Eagles medium with 10% FBS. LNCaP and 22Rv cells were cultured in RPMI 1640 medium with 10% FBS, 1 mM sodium pyruvate, and 10 mM Hepes. PC3 cells have been cultured in F12 Kaighns medium with 5% FBS. Du145 cells had been cultured in minimal crucial medium with 5% FBS, NaHCO3, 0. 1 mM nonessential amino acids, and 1 mM sodium pyruvate. Inside the cell proliferation assay, 5 ? 104 cells have been seeded inside a 6 effectively plate and cultured at 37 C in normoxia. Twenty 4 hrs following seeding, cells utilized in hypoxia experiments were incubated at 37 C in a sealed hypoxia chamber filled with 1% O2, 5% CO2, and 94% N2.. Cells made use of for oligomycin treatment had been incubated at 37 C underneath normoxic situation. To generate the PKM2 rescue H1299 cell lines, ...
JCI - NK cell heparanase controls tumor invasion and immune surveillanceJCI - NK cell heparanase controls tumor invasion and immune surveillance
Enrichment of human cells from PBMCs. PBMCs were isolated from buffy coats and blood using Ficoll-Paque Premium (GE Healthcare). For the generation of iDCs, PBMCs were resuspended in HBSS (Invitrogen, Thermo Fisher Scientific) containing 5% heat-inactivated fetal calf serum (HIFCS) and incubated for 30 to 45 minutes. Adherent monocytes were cultured in mixed leukocyte culture-conditioned (MLC-conditioned) media (consisting of DMEM supplemented with 4 mg/ml D-glucose, 6 μg/ml folic acid, 3.6 μg/ml l-asparagine, 116 μg/ml l-arginine, 3.7 mg/ml NaHCO3, 10% FCS, 2 mM l-glutamine, 1 mM HEPES, 1 mM sodium pyruvate, 0.1 mM 2-mercaptoethanol, 100 U/ml penicillin, 50 μg/ml streptomycin, and 100 μg/ml neomycin) supplemented with 50 ng/ml granulocyte macrophage-CSF (GM-CSF) and 20 ng/ml IL-4. On day 4, nonadherent monocyte-derived i-DCs were purified by FACS sorting of CD3-CD14-CD56-CD19-CD209+ cells on a BD FACS Vantage SE Diva Option Sorter (BD Biosciences). NK cells were isolated by negative ...
JCI - NK cell heparanase controls tumor invasion and immune surveillanceJCI - NK cell heparanase controls tumor invasion and immune surveillance
Enrichment of human cells from PBMCs. PBMCs were isolated from buffy coats and blood using Ficoll-Paque Premium (GE Healthcare). For the generation of iDCs, PBMCs were resuspended in HBSS (Invitrogen, Thermo Fisher Scientific) containing 5% heat-inactivated fetal calf serum (HIFCS) and incubated for 30 to 45 minutes. Adherent monocytes were cultured in mixed leukocyte culture-conditioned (MLC-conditioned) media (consisting of DMEM supplemented with 4 mg/ml D-glucose, 6 μg/ml folic acid, 3.6 μg/ml l-asparagine, 116 μg/ml l-arginine, 3.7 mg/ml NaHCO3, 10% FCS, 2 mM l-glutamine, 1 mM HEPES, 1 mM sodium pyruvate, 0.1 mM 2-mercaptoethanol, 100 U/ml penicillin, 50 μg/ml streptomycin, and 100 μg/ml neomycin) supplemented with 50 ng/ml granulocyte macrophage-CSF (GM-CSF) and 20 ng/ml IL-4. On day 4, nonadherent monocyte-derived i-DCs were purified by FACS sorting of CD3-CD14-CD56-CD19-CD209+ cells on a BD FACS Vantage SE Diva Option Sorter (BD Biosciences). NK cells were isolated by negative ...
After staining, the slides were washed with running deionized water and dehydrated in an ethanol series | GHSR Inhibitor...After staining, the slides were washed with running deionized water and dehydrated in an ethanol series | GHSR Inhibitor...
An suitable amount of root suggestions had been chopped in extraction buffer (.01 M MgSO4, 5 mM KCl, .5 mM Hepes, one mg/ml dithiothreitol, and .25% Triton
Mta connection to the server was lost - Hosting BlogMta connection to the server was lost - Hosting Blog
The transition medium was either M199 medium containing Earles salts and glutamine (Invitrogen), buffered with 150 mM HEPES and adjusted to pH 7. That was years mta connection to the server was lost and my experience over time remained generally good. For example, if you run out of PHP memory or you want to stress test your site to be prepared for traffic spikesyou wont be able to resolve this on your own. Use Stripe as a virtual device type. With 128MB, your VPS will swap directly when it boots, and with 128MB memory 128MB swap is not enough for spamassassin to work properly if you receive heavy emails. McFadden is hosted exchange server for small business international award-winning photographer, with pieces currently touring in Europe, as well as Elizabeth City State UniversityВs permanent art collection. Hi There my name is ahmer and I am in the process of purchasing a smart TV and i want to watch BBC and US flm channels. Skip to the next section if you have already installed your first ...
December, 2016 | IDH Inhibitor idhinhibitor.comDecember, 2016 | IDH Inhibitor idhinhibitor.com
Briefly, a whole of a thousand mg whole mobile extract of cells transfected with pCMX, pCMX-PDX-1 V5 or MEDChem Express 153436-53-4 pCMX-PDX-one D1-37 V5, was incubated with 2 mg biotinylated double stranded oligonucleotide for 4 several hours with continuous rotation at 4uC in a complete volume of 800 ml immunoprecipitation (IP) buffer (fifty mM HEPES, […]. Continue reading ...
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Widespread Inhibition of Sodium Channel-dependent Glutamate Release from Isolated Nerve Terminals by Isoflurane and Propofol |...Widespread Inhibition of Sodium Channel-dependent Glutamate Release from Isolated Nerve Terminals by Isoflurane and Propofol |...
Crude synaptosomes (P2 fraction) were prepared from striatum, hippocampus, or cerebral cortex of young adult male Sprague-Dawley rats (150-200 g), from cerebral cortex of adult male C57BL/6 mice (25-30 g), or from cerebral cortex of adult male guinea pigs (400-500 g) as described. 26 Synaptosomes were suspended in HEPES buffered medium (composition: 140 mm NaCl, 5 mm KCl, 1 mm MgCl2, 1.2 mm Na2HPO4, 5 mm NaHCO3, 10 mm d-glucose, and 20 mm HEPES/NaOH, pH 7.4), collected by centrifugation at 8,000 g for 10 min, and stored on ice for up to 5 h until use. Release of endogenous glutamate was measured by an enzyme-linked fluorometric method. 27 Synaptosomal pellets (0.5 mg protein, determined by the method of Bradford 28) were resuspended in HEPES buffered medium plus 16 μm bovine serum albumin, 1 mm NADP+, 100 U l-glutamate dehydrogenase, and 1.3 mm CaCl2(1.5 ml final volume). Stirred samples were equilibrated at 37°C for 4 min in a spectrofluorometer cuvette, and data acquisition was initiated ...
Cation effects on cell shape.<...Cation effects on cell shape.<...
TY - JOUR. T1 - Cation effects on cell shape.. AU - Sheetz, Michael. PY - 1977/12/1. Y1 - 1977/12/1. N2 - We have found that human erythrocyte ghosts in 10 mM HEPES (pH 7.0) at 0 degrees C would crenate when 20-50 mM of Na+ or K+, 0.2-0.5 mM OF Ca++, Ba++, Sr++, or Mg++, or 10 muM of La+++ was added. The shape change after cation addition was faster than fixation by 1% glutaraldehyde at 4 degrees C and was readily reversible upon dilution of the cation. After incubation of ghosts in 10 mM HEPES (pH 7.0) at 37 degrees for 10-20 min there was a significant inhibition of subsequent crenation by cations. In a process that is believed to occur by a similar mechanism, whole red blood cells were observed to cup (invaginate) when 20 mM of a divalent or 0.1 mM of a trivalent cation was added. After neuraminidase treatment to remove the sialic acid charge groups, these same shape changes were observed in ghosts and whole cells. Another type of cation-induced crenation was found to follow upon the addition ...
Pleiotropic effects of apolipoprotein A-Ⅱ on high-density lipoprotein functionality, adipose tissue metabolic activity and...Pleiotropic effects of apolipoprotein A-Ⅱ on high-density lipoprotein functionality, adipose tissue metabolic activity and...
Lipoprotein-associated phospholipase A2 (Lp-PLA2) activity was measured by a modification of the trichloroacetic acid precipitation procedure in plasma using [3H]-PAF (1-O-hexadecyl-2-[3H-acetyl]-sn-glycero-3-phosphocholine) (10 Ci/mmol, DuPont-New England Nuclear, Boston, MA, USA) as a substrate at a final concentration of 100 μmol/L, as described previously[24]. Briefly, 50 μL diluted plasma (1∶25 v/v with HEPES pH7.4 buffer which consists of 4.2 mmol/L HEPES, 137 mmol/L NaCl, 2.6 mmol/L KCl, and 2 mmol/L EDTA) as the source of the enzyme or blanks (for determination of background) were mixed with assay buffer (HEPES) to a final volume of 90 μL. The reaction was allowed to proceed for 10 minutes at 37 °C by addition of 10 μL of working substrate solution (2 mmol/L [3Η]-PAF in 2.5×103 mg/L BSA, prepared fresh daily). The reaction was terminated by addition of 20 μL ice-cold aqueous bovine serum albumin (BSA) solution (100×103 mg/L) followed by vortex-mixing and incubation for 10 ...
JCI - Regulation of expression of the multidrug resistance protein MRP1 by p53 in human prostate cancer cellsJCI - Regulation of expression of the multidrug resistance protein MRP1 by p53 in human prostate cancer cells
Cell Culture. LNCaP.FGC 1740 cells frozen at passage 18 (American Type Culture Collection, Rockville, Maryland, USA) were routinely cultured in RPMI 1640 supplemented with 10% FBS, 15 mM HEPES buffer (pH 7.4), 2 mM L-glutamine and 100 μM nonessential amino acids (GIBCO BRL, Gaithersburg, Maryland, USA). LVCaP cells were routinely cultured in the LNCaP media supplemented with 50 μg/mL of Geneticin (GIBCO BRL).. The human melanoma cell lines, A875 and A875/E6, were kindly provided by M. Murphy (Fox Chase Cancer Center, Philadelphia, Pennsylvania, USA). The A875/E6 line harbors human papilloma virus (HPV)16E6. The human colon cancer cell lines RKO and RKO.mp53.13 were generous gifts from M. Kastan (St. Jude Childrens Research Hospital, Memphis, Tennessee, USA). RKO.mp53.13 was generated by transfecting parental RKO cells with a dominant-negative p53 mutant. Cells were maintained in a humidified atmosphere containing 5% CO2/95% air and were free of contamination with mycoplasma or ...
USE OF 5-H-DIBENZ/B,F/AZEPINE-5-CARBOXAMIDE DERIVATIVES IN THE TREATMENT OF NEUROPATHIC PAIN AND NEUROLOGICAL DISORDERS -...USE OF 5-H-DIBENZ/B,F/AZEPINE-5-CARBOXAMIDE DERIVATIVES IN THE TREATMENT OF NEUROPATHIC PAIN AND NEUROLOGICAL DISORDERS -...
0069] Blockade of voltage-sensitive sodium channels was studied by investigating [3H] batrachotoxinin A 20-R-benzoate ([3H]BTX) displacement binding to whole brain membranes. Animals were decapitated and their brains quickly removed. Membrane preparation and binding assays were performed essentially as previously described (Shimidzu et al., 1997). Brains (without cerebellum) were homogenised in 10 vol 0.32 M sucrose, 1 mM EDTA, 1 mg/ml bovine serum albumin (BSA), 5 mM HEPES/TRIS pH 7.4 with a Teflon homogeniser (8 strokes at 400 r.p.m). After a 10 min centrifugation at 1,000 g the supernatants were centrifuged for 20 min at 39,000 g and pellets were homogenised with 20 vol or 40 vol Na+-free buffer, respectively for [3H]-batrachotoxinin A 20-alpha-benzoate ([3H]-BTX) binding assays. Na+-free buffer had the following composition (in mM): 130 choline chloride, 0.8 MgSO4, 5.4 KCl, 5.5 D-glucose, 50 HEPES/TRIS, pH 7.4. The homogenate was centrifuged for 20 min at 39,000 g and the resultant pellets ...
COLLAGEN AND FIBRIN MICROTHREADS IN A DISCRETE THREAD MODEL OF IN VITRO ACL SCAFFOLD REGENERATION - Patent applicationCOLLAGEN AND FIBRIN MICROTHREADS IN A DISCRETE THREAD MODEL OF IN VITRO ACL SCAFFOLD REGENERATION - Patent application
0110]Fibrin microthread preparation: Fibrin microthreads were co-extruded from solutions of fibrinogen and thrombin according to the schematic shown in FIG. 1. Fibrinogen from bovine plasma (Sigma, St. Louis, Mo., catalogue number F4753) was dissolved in HEPES Buffered Saline (HBS, 20 mM HEPES, 0.9% NaCl) at 70 mg/mL and stored at -20° C. Thrombin from bovine plasma (Sigma, St. Louis, Mo., catalogue number T4648) was stored frozen as a stock solution at a concentration of 40 U/mL in HBS. A working solution of thrombin was diluted from the stock to a final concentration of 6 U/mL in a 40 mM CaCl2 solution. Both the fibrinogen and thrombin solutions were warmed to 37° C. and placed into separate 1 mL syringes. The solutions were coextruded using a stabilized crosshead on a threaded rod with a crosshead speed of 4.25 mm/min through a blending applicator tip (Micromedics, Inc., St. Paul, Minn.). The blending applicators were Luer locked to the two syringes through individual bores and mixed in a ...
Chen medium Summary Report | CureHunterChen medium Summary Report | CureHunter
Chen medium: contains dextran, chondroitin sulfate, HEPES buffer, gentamicin, streptomycin sulfate, sodium carbonate, nonessential amino acids, vitamins; used for corneal storage
Kinetic characterization of the interaction of the Z-fragment of protein A with mouse-IgG3 in a volume in chemical space.Kinetic characterization of the interaction of the Z-fragment of protein A with mouse-IgG3 in a volume in chemical space.
The kinetic rate parameters for the interaction between a single domain analogue of staphylococcal protein A (Z) and a mouse-IgG3 monoclonal antibody (MAb) were measured in Hepes buffer with different chemical additives. Five buffer ingredients (pH, NaCl, DMSO, EDTA, and KSCN) were varied simultaneously in 16 experiments following a statistical experimental plan. The 16 buffers thus spanned a volume in chemical space. A mathematical model, using data from the buffer composition, was developed and used to predict apparent kinetic parameters in five new buffers within the spanned volume. Association and dissociation parameters were measured in the new buffers, and these agreed with the predicted values, indicating that the model was valid within the spanned volume. The pattern of variation of the kinetic parameters in relation to buffer composition was different for association and dissociation, such that pH influenced both association and dissociation and NaCl influenced only dissociation. This ...
SureFire Optimized Bolt Carrier-Long Stroke (OBC-LS) Drop-In BCG and H7S Buffer System with Longer Action Spring for Better...SureFire Optimized Bolt Carrier-Long Stroke (OBC-LS) Drop-In BCG and H7S Buffer System with Longer Action Spring for Better...
DR is curious as to how the SureFire Optimzed Bolt Carrier/H7S Buffer combo stacks up against both the FERFRANS DSAS/RRS (Delayed Sear Activation System/Rate Reduction System) BCG and Nemo Arms RRS BCG, respectively. Weve been writing about the FERFRANS BCG for years, and were BIG fans of it. Both the OBC-LS/H7 Buffer System and DSAS/RRS BCG essentially achieve a similar effect through two completely different designs. Where the SureFire OBC-LS/H7 Buffer System utilizes a springloaded weight in the back of the carrier and teams the BCG up with a proprietary buffer, the FERFRANS DSAS/RRS BCG utilizes a simple reciprocating metal piece that recipricates freely within the BCG, via inertia. The the OBC-LS/H7 Buffer System is a more sophisticated design than the FERFRANS DSAS/RRS BCG, so it will be interesting to see how they compare back-to-back at some point.. DR has only examined and written about the Nemo Arms RRS BCG once when it was pulled out of a Nemo Arms Omen Recon .300WM 18″ ...
A Dihydropyridine-sensitive Voltage-dependent Calcium Channel in the Sarcolemmal Membrane of Crustacean Muscle | JGPA Dihydropyridine-sensitive Voltage-dependent Calcium Channel in the Sarcolemmal Membrane of Crustacean Muscle | JGP
Standard electrophysiological techniques were used for potential recording and current injection under current-clamp conditions (Erxleben et al., 1995). For two-electrode voltage-clamp experiments, an Axoclamp 2B (Axon Instruments Inc., Foster City, CA) was used. These experiments were performed on fibers of the last abdominal segment which are compact (270-450 μm long with a diameter of 50-80 μm). Injection of current in the middle of the fibers and recording at several points over the length of the fiber showed that they are isopotential and thus suitable for two-electrode voltage-clamp. For isolation of Ca or Ba currents and suppression of K currents, a solution (TEA solution) consisting of 160 mM tetraethyl-ammonium, 2 mM 4-aminopyridine (4-AP), 320 mM NaCl, 8 mM KCl, 20 mM HEPES, pH 7.4, was used with either 10 mM CaCl2 or BaCl2. In addition, the recording and current electrodes were filled with 3 M CsCl to suppress K currents. Voltage-activated Ca or Ba currents were separated from ...
Conditional, Genetically Encoded, Small Molecule-Regulated Inhibition of NFκB Signaling in RPE Cells | IOVS | ARVO JournalsConditional, Genetically Encoded, Small Molecule-Regulated Inhibition of NFκB Signaling in RPE Cells | IOVS | ARVO Journals
For typical Western blotting experiments, (e.g., Fig. 2J-L), cells were plated at 200,000 cells per well of a 12-well plate in complete Dulbeccos modified Eagles medium (DMEM)/F12 media containing 10% serum. The next day, cells were treated as indicated. For Western blotting involving production of cytokines, ARPE-19 cells were plated as described above followed by gradual serum removal over the course of a week until the serum was removed completely followed by indicated treatments. At appropriate time points, ARPE-19 cells were lysed in-plate with either radioimmunoprecipitation assay (RIPA) buffer (50 mM Tris, pH 7.4, 150 mM NaCl2, 1% Triton X-100 [vol/vol], 0.1% SDS [wt/vol], 0.5% sodium deoxycholate [wt/vol]) supplemented with Halt Protease and Phosphatase inhibitors (Pierce, Rockford, IL, USA) and benzonase (Sigma-Aldrich Corp.) or a buffer containing 50 mM HEPES pH 8, 10 mM KCl, 2 mM MgCl2, and 1.0% SDS also supplemented with benzonase. Cells were lysed at RT for approximately 1 to 2 ...
Modulation of Tight Junction Proteins in the Perineurium to Facilitate Peripheral Opioid Analgesia | Anesthesiology | American...Modulation of Tight Junction Proteins in the Perineurium to Facilitate Peripheral Opioid Analgesia | Anesthesiology | American...
In isoflurane-anesthetized rats, 10% NaCl (total volume 300 μl) was injected subcutaneously on the medial aspect of the foot and medial aspect of the rat lower limb, which is innervated by the saphenous nerve. After 30 min, an in vitro skin-nerve preparation was dissected and used to record from single primary afferents in microdissected teased filaments of the saphenous nerve, as previously described.18The skin-nerve preparation was perfused at 15 ml/min with oxygen-saturated synthetic interstitial fluid buffer containing 123 mM NaCl, 3.5 mM KCl, 0.7 mM MgSO4, 1.7 mM NaH2PO4, 2.0 mM CaCl2, 9.5 mM sodium gluconate, 5.5 mM glucose, 7.5 mM sucrose, and 10 mM HEPES at pH 7.4. The mechanical receptive fields of individual units were identified by manually probing the skin with a glass rod. Αδ- and C-fiber mechanonociceptors were identified by their conduction velocity and threshold to mechanical stimulation by von Frey hairs, as described previously.3Stock solutions of DAMGO and CTOP were diluted ...
Indian Patents. 214369:A DIBENZOAZULENE COMPOUNDIndian Patents. 214369:A DIBENZOAZULENE COMPOUND
In order to obtain peritoneal macrophages, Balb/C mouse strain males, age 8 to 12 weeks, were injected i.p. with 300 fig of zymosan (SIGMA) dissolved in a phosphate buffer (PBS) in a total volume of 0.1 ml/mouse. After 24 hours the mice were euthanized according to the Laboratory Animal Welfare Act. The peritoneal cavity was washed with a sterile physiological solution (5 ml). The obtained peritoneal macrophages were washed twice with a sterile physiological solution and, after the last centrifugation (350 g/10 min), resuspended in RPMI 1640, into which 10% of FBS were added. In order to determine TNF-a secretion, SxlO4 cells/well were cultivated in a total volume of 200 \x\ for 18 to 24 hours on midrotitre plates with a flat bottom (96 wells. Falcon) in RPMI 1640 medium, into which 10% FBS (Fetal Bovine Serum, Biowhittaker) inactivated by heat, 100 units/ml of penicillin, 100 mg/ml of streptomycin, 20 mM HEPES and 50 μM 2-mercaptoethanol (all of GIBCO) were added. The cells were incubated at ...
Plus itPlus it
The functional characterization of methaqualone at wild-type (WT) and mutant GABAARs expressed in Xenopus oocytes was performed essentially as previously described (Hoestgaard-Jensen et al., 2013). The GABAAR cDNAs were linearized and applied as templates for in vitro cRNA synthesis using the T7 mMESSAGE mMACHINE High Yield Capped RNA transcription kit (Life Technologies Corp., Carlsbad, CA. Either 9 or 18 nl cRNA encoding for α1β2 (α1:β2 ratio: 0.06:0.06 µg/µl) and α1,2,3,5β2,3γ2S GABAARs (α:β:γ2S ratio: 0.01:0.01:0.01 µg/μl), or 46 nl cRNA encoding for α6β2 (α6:β2 ratio: 1:0.1 µg/µl) and α4,6β1,2.3δ GABAARs (α:β:δ ratio: 1:0.1:1 µg/μl) were injected into oocytes, which subsequently were incubated at 18°C in modified Barths solution [88 mM NaCl, 1 mM KCl, 15 mM HEPES (pH 7.5), 2.4 mM NaHCO3, 0.41 mM CaCl2, 0.82 mM MgSO4, 0.3 mM Ca(NO3)2, 100 U/ml penicillin and 100 μg/ml streptomycin]. Whole-cell currents in the α1β2/α1,2,3,5β2,3γ2S- and ...
2-{[(3α,5β,6α,7α,17ξ)-3,6,7-Trihydroxy-24-oxocholan-24-yl]amino}ethanesulfonic acid | C26H45NO7S | ChemSpider2-{[(3α,5β,6α,7α,17ξ)-3,6,7-Trihydroxy-24-oxocholan-24-yl]amino}ethanesulfonic acid | C26H45NO7S | ChemSpider
Structure, properties, spectra, suppliers and links for: 2-{[(3α,5β,6α,7α,17ξ)-3,6,7-Trihydroxy-24-oxocholan-24-yl]amino}ethanesulfonic acid.
2-{[(3α,5β,12α,17α,20S)-3,12-Dihydroxy-24-oxocholan-24-yl]amino}ethanesulfonic acid | C26H45NO6S | ChemSpider2-{[(3α,5β,12α,17α,20S)-3,12-Dihydroxy-24-oxocholan-24-yl]amino}ethanesulfonic acid | C26H45NO6S | ChemSpider
Structure, properties, spectra, suppliers and links for: 2-{[(3α,5β,12α,17α,20S)-3,12-Dihydroxy-24-oxocholan-24-yl]amino}ethanesulfonic acid.
ERK1 Antibody (Monoclonal, 33A5)ERK1 Antibody (Monoclonal, 33A5)
ERK1 Monoclonal Antibody from Invitrogen for Western Blot and ELISA applications. This antibody reacts with Human, Rat samples. Clone: 33A5. Supplied as 100 µL purified antibody in HEPES with 0.15M NaCl, 0.01% BSA, 50% glycerol and 0.03% sodium azide.
how long can it take for herpes simplex 2 to show up. how can i prevent future beeakouts? | Answers from Doctors | HealthTaphow long can it take for herpes simplex 2 to show up. how can i prevent future beeakouts? | Answers from Doctors | HealthTap
Dr. Addagada Rao answered: 2 to 7 days: Will take less than a wk to hepes simplex ii infection , once get infected stays in the body for ever , anti viral ...
What is a buffer? PreLab 3.5What is a buffer? PreLab 3.5
A buffer is a mixture of molecules that release or bind H+ in order to maintain a relatively stable pH. Note that the function of a buffer is NOT to keep a solution neutral (at pH 7); its function is to minimize the change in pH when base or acid is added to the solution. Also note that there are many different buffers, and each one will stabilize the pH of a solution only within a specific pH range. One buffer may be effective within a range of pH 2 to pH 6, while another may be effective within a range of pH 10 to pH 12. Beyond its buffering range, a buffer no longer acts to stabilize the pH of the solution ...
HEPPS (molecule)HEPPS (molecule)
Reactive oxygen species production in marine microalgaeReactive oxygen species production in marine microalgae
YepesYepes
Carbamoyl phosphate synthetaseCarbamoyl phosphate synthetase
MOPSMOPS
Diethyl pyrocarbonateDiethyl pyrocarbonate
Goods buffersGood's buffers
PIPESPIPES
Cell isolationCell isolation
TricineTricine
TrisTris
MES (buffer)MES (buffer)
TransfectionTransfection
HEPBSHEPBS
NUN bufferNUN buffer
Saline (medicine)Saline (medicine)
Lysis bufferLysis buffer
Particle-induced X-ray emissionParticle-induced X-ray emission
Hyperpolarized carbon-13 MRIHyperpolarized carbon-13 MRI
Minusheet perfusion culture systemMinusheet perfusion culture system
List of MeSH codes (D03)List of MeSH codes (D03)
List of MeSH codes (D02)List of MeSH codes (D02)
HBSHBS
Crystallization adjutantCrystallization adjutant
Fixation (histology)Fixation (histology)
EicosanoidEicosanoid
Wikipedia:WikiProject Chemicals/Log/2010-01-28Wikipedia:WikiProject Chemicals/Log/2010-01-28
ACP1ACP1
HEPESHEPES
Buffer solutionBuffer solution
Szerves vegyületek listája - WikipédiaSzerves vegyületek listája - Wikipédia
AnatomyOrganismsDiseasesChemicals and DrugsAnalytical, Diagnostic and Therapeutic Techniques and EquipmentPhenomena and ProcessesDisciplines and OccupationsAnthropology, Education, Sociology and Social PhenomenaTechnology, Industry, AgricultureInformation ScienceNamed GroupsHealth CareGeographicals
HEPESBuffersTromethamineAmino Acids, NeutralBicarbonatesHydrogen-Ion Concentration4,4'-Diisothiocyanostilbene-2,2'-Disulfonic AcidFraxinusLichensGermanyPhysician AssistantsSaltsSodiumPhenolsulfonphthaleinPhenolphthaleinsPhenolCulture MediaCell LineCricetinaeCarbonic Anhydrase ICatalogs, LibraryDNA DamageCatalogs as TopicProstaglandins F, SyntheticPhysical FitnessOrganic ChemicalsNanoparticlesMultiple MyelomaPublicationsGlutamineClick ChemistryPeriodicals as TopicBibliometricsJournal Impact FactorContainment of BiohazardsSurgery, VeterinaryAutonomic PathwaysSinoatrial NodeSearch EngineBiofuelsMolecular BiologyTerminology as TopicNuclear Magnetic Resonance, BiomolecularProtonsProton Pumps
HEPES Sodium Salt Specification Comparison | China-Mainland | Sigma-AldrichHEPES Sodium Salt Specification Comparison | China-Mainland | Sigma-Aldrich
DMEM, high glucose, HEPES, no phenol redDMEM, high glucose, HEPES, no phenol red
HEPES, Free Acid, High Purity | BioExpressHEPES, Free Acid, High Purity | BioExpress
HEPES, Free Acid | CAS 7365-45-9 | Goods Buffer | P212121 StoreHEPES, Free Acid | CAS 7365-45-9 | Good's Buffer | P212121 Store
SuperCult® RPMI 1640 without HEPES and L-glutamine | Creative BioarraySuperCult® RPMI 1640 without HEPES and L-glutamine | Creative Bioarray
Entecavir | HIV Infection & Hepes Treatment | Online PharmacyEntecavir | HIV Infection & Hepes Treatment | Online Pharmacy
bmse000792 HEPES - Supplemental Proton Data at BMRBbmse000792 HEPES - Supplemental Proton Data at BMRB
Gentaur Molecular :Biosera \ Iscoves Modified DMEM w L Glutamine w 25 mM Hepes w o Phenol Red For 1L \ LM...Gentaur Molecular :Biosera \ Iscove's Modified DMEM w L Glutamine w 25 mM Hepes w o Phenol Red For 1L \ LM...
He catalytic activity; replacement of HEPES/KOH buffer with TRIS/HCl | CTSK Inhibito ctskinhibito.comHe catalytic activity; replacement of HEPES/KOH buffer with TRIS/HCl | CTSK Inhibito ctskinhibito.com
P0192-N1L - IMDM w/ L-Glutamine w/ 25 mM Hepes w/o Phenol Red - Jain BiologicalsP0192-N1L - IMDM w/ L-Glutamine w/ 25 mM Hepes w/o Phenol Red - Jain Biologicals
HEPES (Fine White Crystals/Molecular Biology), Fisher BioReagents Poly Bottle; 500g HEPES (Fine White Crystals/Molecular...HEPES (Fine White Crystals/Molecular Biology), Fisher BioReagents Poly Bottle; 500g HEPES (Fine White Crystals/Molecular...
HEPES - WikipediaHEPES - Wikipedia
HEPES-bufferHEPES-buffer
HEPES Specification Comparison | China-Mainland | Sigma-AldrichHEPES Specification Comparison | China-Mainland | Sigma-Aldrich
1M HEPES Buffer1M HEPES Buffer
Precise™ Tris-HEPES Gels | Thermo Fisher Scientific - CAPrecise™ Tris-HEPES Gels | Thermo Fisher Scientific - CA
HEPES Buffer Solution (1M) | STEMCELL TechnologiesHEPES Buffer Solution (1M) | STEMCELL Technologies
Alfa Aesar HEPES Lysis Buffer with NP-40 250mL:Life SciencesAlfa Aesar HEPES Lysis Buffer with NP-40 250mL:Life Sciences
Efflux of cyclic AMP from resealed erythrocyte ghosts is not enhanced by hepes | SpringerLinkEfflux of cyclic AMP from resealed erythrocyte ghosts is not enhanced by hepes | SpringerLink
SIGMA-ALDRICH Hepes,Contain 250g,CAS 7365-45-9,Glass - 45ZE86|H3375-250G - GraingerSIGMA-ALDRICH Hepes,Contain 250g,CAS 7365-45-9,Glass - 45ZE86|H3375-250G - Grainger
HEPES BUFFER and protein degradation - Protein and Proteomics - BioForumHEPES BUFFER and protein degradation - Protein and Proteomics - BioForum
HEPES sodium salt | BioExpressHEPES sodium salt | BioExpress
HBSS with 10 mM HEPES, Without Phenol Red | STEMCELL TechnologiesHBSS with 10 mM HEPES, Without Phenol Red | STEMCELL Technologies
7365-45-9 - HEPES, 0.5M buffer soln., pH 7.0 - J60064 - Alfa Aesar7365-45-9 - HEPES, 0.5M buffer soln., pH 7.0 - J60064 - Alfa Aesar
HEPES Buffered Saline Solution | PromoCellHEPES Buffered Saline Solution | PromoCell
HEPES, Free Acid, ULTROL™ Grade, 1.0 M Solution, Calbiochem™, EMD MilliporeHEPES, Free Acid, ULTROL™ Grade, 1.0 M Solution, Calbiochem™, EMD Millipore
AID 357113 - Antibacterial activity against Escherichia coli K12 ATCC 23716 in 10% Luria-Bertani medium/90% Hepes buffer at pH...AID 357113 - Antibacterial activity against Escherichia coli K12 ATCC 23716 in 10% Luria-Bertani medium/90% Hepes buffer at pH...
DMEM / Hams F-12 w/ L-glutamine and 15mM HEPES w/o cholineDMEM / Ham's F-12 w/ L-glutamine and 15mM HEPES w/o choline
HEPES Buffered Saline Solution 2X | CAS 7365-45-9 | Buffer | AG Sci.HEPES Buffered Saline Solution 2X | CAS 7365-45-9 | Buffer | AG Sci.
Concentrative transport of antifolates mediated by the proton-coupled folate transporter (PCFT, SLC46A1); augmentation by a...Concentrative transport of antifolates mediated by the proton-coupled folate transporter (PCFT, SLC46A1); augmentation by a...
AID 536873 - Binding affinity to human antithrombin in HEPES buffer containing 0.25 M sodium chloride assessed as increase of...AID 536873 - Binding affinity to human antithrombin in HEPES buffer containing 0.25 M sodium chloride assessed as increase of...
HEPES Hemisodium | CAS 103404-87-1 | Buffer | P212121 StoreHEPES Hemisodium | CAS 103404-87-1 | Buffer | P212121 Store
Hydrogen peroxide formation by reaction of peroxynitrite with HEPES and related tertiary amines. Implications for a general...Hydrogen peroxide formation by reaction of peroxynitrite with HEPES and related tertiary amines. Implications for a general...
Search results for: HEPES bufferSearch results for: 'HEPES buffer'
ХЕПЕС(HEPES), LS, Panreac, 50 гХЕПЕС(HEPES), LS, Panreac, 50 г
AnatomyOrganismsDiseasesChemicals and DrugsAnalytical, Diagnostic and Therapeutic Techniques and EquipmentPhenomena and ProcessesDisciplines and OccupationsAnthropology, Education, Sociology and Social PhenomenaTechnology, Industry, AgricultureInformation ScienceNamed GroupsHealth CareGeographicals
HEPESBuffersTromethamineAmino Acids, NeutralBicarbonatesHydrogen-Ion Concentration4,4'-Diisothiocyanostilbene-2,2'-Disulfonic AcidFraxinusLichensGermanyPhysician AssistantsSaltsSodiumPhenolsulfonphthaleinPhenolphthaleinsPhenolCulture MediaCell LineCricetinaeCarbonic Anhydrase ICatalogs, LibraryDNA DamageCatalogs as TopicProstaglandins F, SyntheticPhysical FitnessOrganic ChemicalsNanoparticlesMultiple MyelomaPublicationsGlutamineClick ChemistryPeriodicals as TopicBibliometricsJournal Impact FactorContainment of BiohazardsSurgery, VeterinaryAutonomic PathwaysSinoatrial NodeSearch EngineBiofuelsMolecular BiologyTerminology as TopicNuclear Magnetic Resonance, BiomolecularProtonsProton Pumps
AcidGlobal HEPES marketConcentration238.3ZwitterionicBuffer solutionTrisNaOHBiologicalAbsorbanceQuantificationGlutamineSalinePhysiological pHAssayConcentrationsCommonlyRoom TemperatureMagnesiumSaltCell cultureSuitableSolutionsWaterMethod
Acid4
  • HEPES (N-2-hydroxyethylpiperazine-N-2-ethane sulfonic acid) is a general purpose zwitterionic organic chemical buffering agent which does not bind magnesium, calcium, manganese(II) or copper (II) ions. (mpbio.com)
  • 1) Hegetschweiler and Saltman, Interaction of copper(II) with N-(2-hydroxyethyl)piperazine-N'-ethanesulfonic acid (HEPES), Inorg. (p212121.com)
  • In this work, three-dimensional branched gold nanocrystals were produced at high yield by reacting an aqueous solution of chloroauric acid with a Good's buffer, HEPES, at room temperature. (p212121.com)
  • A HEPES that is ethanesulfonic acid in which one of the methyl hydrogens is replaced by a 4-(2-hydroxyethyl)piperazin-1-yl group. (ebi.ac.uk)
Global HEPES market5
Concentration2
  • Studies have indicated that 20 mM HEPES is the most satisfactory concentration of the buffer when both Hanks' and Earle's solutions are used. (mpbio.com)
  • Maximum activation was reached at different K+ concentrations depending on the buffering species: in TRIS/HCl buffer, 100 mM K+ was the most effective, whereas in HEPES/KOH buffer, maximum activity was reached at about 200 mM K+ concentration. (ctskinhibito.com)
238.31
Zwitterionic2
Buffer solution3
Tris5
NaOH1
  • A simple mixing table for preparing 0.05 M HEPES/NaOH has been published. (mpbio.com)
Biological1
Absorbance1
  • HEPES is a good buffering choice for many cell culture systems because it is membrane impermeable, has limited effect on biochemical reactions, is chemically and enzymatically stable, and has very low visible and UV light absorbance. (mpbio.com)
Quantification2
Glutamine1
  • This makes HEPES RPMI 25 milli-molar media with or without L-glutamine a more effective buffering agent for maintaining enzyme structure and function at low temperatures. (gentaur.com)
Saline1
Physiological pH1
Assay1
  • HEPES is the recommended buffer for the glutamate binding assay because it prevents binding to non-receptor materials. (mpbio.com)
Concentrations2
Commonly1
Room Temperature1
Magnesium1
Salt1
Cell culture3
Suitable1
  • HEPES has been evaluated and shown to be suitable for use with Ampholines in generating pH gradients less than 1 pH unit wide for isolectric focusing applications. (mpbio.com)
Solutions2
Water1
  • HEPES is like water in that its dissociation decreases as the temperature decreases. (wikipedia.org)
Method1
  • Furthermore, evaluation of HEPES contents using both TLC assays by 28 subjects supported the conclusion that the newly developed TLC method is clearly favorable. (ejnmmigateway.net)
Entecavir | HIV Infection & Hepes Treatment | Online Pharmacy
Entecavir | HIV Infection & Hepes Treatment | Online Pharmacy (cheapmedicineshop.com)
Nutritional Supplements
Nutritional Supplements (fishersci.com)
Cell Culture Media | Fisher Scientific
Cell Culture Media | Fisher Scientific (fishersci.com)
Cell Culture Media
Cell Culture Media (fishersci.com)
Pathological α-synuclein impairs adult-born granule cell development and functional integration in the olfactory bulb | Nature...
Pathological α-synuclein impairs adult-born granule cell development and functional integration in the olfactory bulb | Nature... (nature.com)
7365-45-9 - HEPES, 0.5M buffer soln., pH 7.0 - J60064 - Alfa Aesar
7365-45-9 - HEPES, 0.5M buffer soln., pH 7.0 - J60064 - Alfa Aesar (alfa.com)
Cell / Tissue Culture > Buffers Laboratory Product Directory and Equipment Reviews on...
Cell / Tissue Culture > Buffers Laboratory Product Directory and Equipment Reviews on... (selectscience.net)
Oxyphenbutazone promotes cytotoxicity in rats and Hep3B cellsvia suppression of PGE2 and deactivation of Wnt/β-catenin...
Oxyphenbutazone promotes cytotoxicity in rats and Hep3B cellsvia suppression of PGE2 and deactivation of Wnt/β-catenin... (link.springer.com)
2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonic acid (CHEBI:42334)
2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonic acid (CHEBI:42334) (ebi.ac.uk)
A thermophilic microorganism from Deception Island, Antarctica with a thermostable glutamate dehydrogenase activity |...
A thermophilic microorganism from Deception Island, Antarctica with a thermostable glutamate dehydrogenase activity |... (link.springer.com)
The Release of Iron from a Subfraction of Rat Liver Highly Enriched in Endosomal Organelles Requires Both a Functional H+...
The Release of Iron from a Subfraction of Rat Liver Highly Enriched in Endosomal Organelles Requires Both a Functional H+... (link.springer.com)
Isolation of the sulphur reductase and reconstitution of the sulphur respiration of Wolinella succinogenes | SpringerLink
Isolation of the sulphur reductase and reconstitution of the sulphur respiration of Wolinella succinogenes | SpringerLink (link.springer.com)
Inactivation and injury of Escherichia coli in a copper water storage vessel: effects of temperature and pH | SpringerLink
Inactivation and injury of Escherichia coli in a copper water storage vessel: effects of temperature and pH | SpringerLink (link.springer.com)
P53- and Bax-Mediated Apoptosis in Injured Rat Spinal Cord | SpringerLink
P53- and Bax-Mediated Apoptosis in Injured Rat Spinal Cord | SpringerLink (link.springer.com)
Buffering Cosmetic Chemicals | Cosmetic & Personal Care Ingredients | Spectrum Chemical
Buffering Cosmetic Chemicals | Cosmetic & Personal Care Ingredients | Spectrum Chemical (spectrumchemical.com)
Activation of GLP-1 receptor signalling alleviates cellular stresses and improves beta cell function in a mouse model of...
Activation of GLP-1 receptor signalling alleviates cellular stresses and improves beta cell function in a mouse model of... (link.springer.com)
The mitochondrial citrate carrier, SLC25A1, drives stemness and therapy resistance in non-small cell lung cancer | Cell Death &...
The mitochondrial citrate carrier, SLC25A1, drives stemness and therapy resistance in non-small cell lung cancer | Cell Death &... (nature.com)
Pleomorphic bacteria-like structures in human blood represent non-living membrane vesicles and protein particles | Scientific...
Pleomorphic bacteria-like structures in human blood represent non-living membrane vesicles and protein particles | Scientific... (nature.com)
Ultrapure Biochemicals - Alfa Aesar
Ultrapure Biochemicals - Alfa Aesar (alfa.com)
Skin Care Ingredients Dictionary | SkinCeuticals
Skin Care Ingredients Dictionary | SkinCeuticals (skinceuticals.com)
Category:Piperazines - Wikimedia Commons
Category:Piperazines - Wikimedia Commons (commons.wikimedia.org)
Category:Buffers (chemical) - Wikimedia Commons
Category:Buffers (chemical) - Wikimedia Commons (commons.wikimedia.org)
IJMS | Free Full-Text | Frequency-Dependent Multi-Well Cardiotoxicity Screening Enabled by Optogenetic Stimulation | HTML
IJMS | Free Full-Text | Frequency-Dependent Multi-Well Cardiotoxicity Screening Enabled by Optogenetic Stimulation | HTML (mdpi.com)
Metabolic and Antioxidant System Alterations in an Astrocytoma Cell Line Challenged with Mitochondrial DNA Deletion |...
Metabolic and Antioxidant System Alterations in an Astrocytoma Cell Line Challenged with Mitochondrial DNA Deletion |... (link.springer.com)
Integration of Different -omics Technologies Identifies Inhibition of the IGF1R-Akt-mTOR Signaling Cascade Involved in the...
Integration of Different "-omics" Technologies Identifies Inhibition of the IGF1R-Akt-mTOR Signaling Cascade Involved in the... (hindawi.com)
Culture Media - Cell Biology - Life Sciences
Culture Media - Cell Biology - Life Sciences (mpbio.com)
Fisher Scientific
Fisher Scientific (fishersci.com)
TRPV4 channel participates in receptor-operated calcium entry and ciliary beat frequency regulation in mouse airway epithelial...
TRPV4 channel participates in receptor-operated calcium entry and ciliary beat frequency regulation in mouse airway epithelial... (pnas.org)
Plus it
Plus it (jneurosci.org)
Inhibition of the cAMP Pathway Decreases Early Long-Term Potentiation at CA1 Hippocampal Synapses | Journal of Neuroscience
Inhibition of the cAMP Pathway Decreases Early Long-Term Potentiation at CA1 Hippocampal Synapses | Journal of Neuroscience (jneurosci.org)
Abbreviations, Acronyms, and Initialisms - Emerging Infectious Disease journal - CDC
Abbreviations, Acronyms, and Initialisms - Emerging Infectious Disease journal - CDC (wwwnc.cdc.gov)
Plus it
Plus it (jneurosci.org)
In vitro characterization of IroB, a pathogen-associated C-glycosyltransferase | PNAS
In vitro characterization of IroB, a pathogen-associated C-glycosyltransferase | PNAS (pnas.org)
Continuous and Transient Vesicle Cycling at a Ribbon Synapse | Journal of Neuroscience
Continuous and Transient Vesicle Cycling at a Ribbon Synapse | Journal of Neuroscience (jneurosci.org)