A dipolar ionic buffer.
A chemical system that functions to control the levels of specific ions in solution. When the level of hydrogen ion in solution is controlled the system is called a pH buffer.
An organic amine proton acceptor. It is used in the synthesis of surface-active agents and pharmaceuticals; as an emulsifying agent for cosmetic creams and lotions, mineral oil and paraffin wax emulsions, as a biological buffer, and used as an alkalizer. (From Merck, 11th ed; Martindale, The Extra Pharmacopoeia, 30th ed, p1424)
Amino acids with uncharged R groups or side chains.
Inorganic salts that contain the -HCO3 radical. They are an important factor in determining the pH of the blood and the concentration of bicarbonate ions is regulated by the kidney. Levels in the blood are an index of the alkali reserve or buffering capacity.
The normality of a solution with respect to HYDROGEN ions; H+. It is related to acidity measurements in most cases by pH = log 1/2[1/(H+)], where (H+) is the hydrogen ion concentration in gram equivalents per liter of solution. (McGraw-Hill Dictionary of Scientific and Technical Terms, 6th ed)
An inhibitor of anion conductance including band 3-mediated anion transport.
A plant genus of the family OLEACEAE. Members contain secoiridoid glucosides.
Any of a group of plants formed by a symbiotic combination of a fungus with an algae or CYANOBACTERIA, and sometimes both. The fungal component makes up the bulk of the lichen and forms the basis for its name.
Health professionals who practice medicine as members of a team with their supervising physicians. They deliver a broad range of medical and surgical services to diverse populations in rural and urban settings. Duties may include physical exams, diagnosis and treatment of disease, interpretation of tests, assist in surgery, and prescribe medications. (from http://www.aapa.orglabout-pas accessed 2114/2011)
Substances produced from the reaction between acids and bases; compounds consisting of a metal (positive) and nonmetal (negative) radical. (Grant & Hackh's Chemical Dictionary, 5th ed)
A member of the alkali group of metals. It has the atomic symbol Na, atomic number 11, and atomic weight 23.
Red dye, pH indicator, and diagnostic aid for determination of renal function. It is used also for studies of the gastrointestinal and other systems.
A family of 3,3-bis(p-hydroxyphenyl)phthalides. They are used as CATHARTICS, indicators, and COLORING AGENTS.
An antiseptic and disinfectant aromatic alcohol.
Any liquid or solid preparation made specifically for the growth, storage, or transport of microorganisms or other types of cells. The variety of media that exist allow for the culturing of specific microorganisms and cell types, such as differential media, selective media, test media, and defined media. Solid media consist of liquid media that have been solidified with an agent such as AGAR or GELATIN.
Established cell cultures that have the potential to propagate indefinitely.
A subfamily in the family MURIDAE, comprising the hamsters. Four of the more common genera are Cricetus, CRICETULUS; MESOCRICETUS; and PHODOPUS.
A cytosolic carbonic anhydrase isoenzyme primarily expressed in ERYTHROCYTES, vascular endothelial cells, and the gastrointestinal mucosa. EC 4.2.1.-
Injuries to DNA that introduce deviations from its normal, intact structure and which may, if left unrepaired, result in a MUTATION or a block of DNA REPLICATION. These deviations may be caused by physical or chemical agents and occur by natural or unnatural, introduced circumstances. They include the introduction of illegitimate bases during replication or by deamination or other modification of bases; the loss of a base from the DNA backbone leaving an abasic site; single-strand breaks; double strand breaks; and intrastrand (PYRIMIDINE DIMERS) or interstrand crosslinking. Damage can often be repaired (DNA REPAIR). If the damage is extensive, it can induce APOPTOSIS.
Ordered compilations of item descriptions and sufficient information to afford access to them.
Analogs or derivatives of prostaglandins F that do not occur naturally in the body. They do not include the product of the chemical synthesis of hormonal PGF.
The ability to carry out daily tasks and perform physical activities in a highly functional state, often as a result of physical conditioning.
A white, crystalline powder that is commonly used as a pH buffering agent, an electrolyte replenisher, systemic alkalizer and in topical cleansing solutions.
Tax on the net income of an individual, organization, or business.
Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others.
Connective tissue cells which secrete an extracellular matrix rich in collagen and other macromolecules.
A broad class of substances containing carbon and its derivatives. Many of these chemicals will frequently contain hydrogen with or without oxygen, nitrogen, sulfur, phosphorus, and other elements. They exist in either carbon chain or carbon ring form.
Nanometer-sized particles that are nanoscale in three dimensions. They include nanocrystaline materials; NANOCAPSULES; METAL NANOPARTICLES; DENDRIMERS, and QUANTUM DOTS. The uses of nanoparticles include DRUG DELIVERY SYSTEMS and cancer targeting and imaging.
A malignancy of mature PLASMA CELLS engaging in monoclonal immunoglobulin production. It is characterized by hyperglobulinemia, excess Bence-Jones proteins (free monoclonal IMMUNOGLOBULIN LIGHT CHAINS) in the urine, skeletal destruction, bone pain, and fractures. Other features include ANEMIA; HYPERCALCEMIA; and RENAL INSUFFICIENCY.
Copies of a work or document distributed to the public by sale, rental, lease, or lending. (From ALA Glossary of Library and Information Science, 1983, p181)
A non-essential amino acid present abundantly throughout the body and is involved in many metabolic processes. It is synthesized from GLUTAMIC ACID and AMMONIA. It is the principal carrier of NITROGEN in the body and is an important energy source for many cells.
Organic chemistry methodology that mimics the modular nature of various biosynthetic processes. It uses highly reliable and selective reactions designed to "click" i.e., rapidly join small modular units together in high yield, without offensive byproducts. In combination with COMBINATORIAL CHEMISTRY TECHNIQUES, it is used for the synthesis of new compounds and combinatorial libraries.
A publication issued at stated, more or less regular, intervals.
The use of statistical methods in the analysis of a body of literature to reveal the historical development of subject fields and patterns of authorship, publication, and use. Formerly called statistical bibliography. (from The ALA Glossary of Library and Information Science, 1983)
A quantitative measure of the frequency on average with which articles in a journal have been cited in a given period of time.

Transcriptional regulation of the esp genes of enterohemorrhagic Escherichia coli. (1/141)

We have determined that the genes encoding the secreted proteins EspA, EspD, and EspB of enterohemorrhagic Escherichia coli (EHEC) are organized in a single operon. The esp operon is controlled by a promoter located 94 bp upstream from the ATG start codon of the espA gene. The promoter is activated in the early logarithmic growth phase, upon bacterial contact with eukaryotic cells and in response to Ca2+, Mn2+, and HEPES. Transcription of the esp operon seems to be switched off in tightly attached bacteria. The activation process is regulated by osmolarity (induction at high osmolarities), modulated by temperature, and influenced by the degree of DNA supercoiling. Transcription is sigmaS dependent, and the H-NS protein contributes to its fine tuning. Identification of the factors involved in activation of the esp operon and the signals responsible for modulation may facilitate understanding of the underlying molecular events leading to sequential expression of virulence factors during natural infections caused by EHEC.  (+info)

Generation of intracellular pH gradients in single cardiac myocytes with a microperfusion system. (2/141)

This study describes the use of a microperfusion system to create rapid, large regional changes in intracellular pH (pH(i)) within single ventricular myocytes. The spatial distribution of pH(i) in single myocytes was measured with seminaphthorhodafluor-1 fluorescence using confocal imaging. Changes in pH(i) were induced by local external application of NH(4)Cl, CO(2), or sodium propionate. Local application was achieved by simultaneously directing two parallel square microstreams, each 275 microm wide, over a single myocyte oriented perpendicular to the direction of flow. One stream contained the control solution, and the other contained a weak acid or base. End-to-end, stable pH(i) gradients as large as 1 pH unit were readily created with this technique. This result indicates that pH within a single cardiac cell may not always be spatially uniform, particularly when weak acid or base gradients are present, which can occur, for example, in regional myocardial ischemia. The microperfusion method should be useful for studying the effects of localized acidosis on myocyte function, estimating intracellular ion diffusion rates, and, possibly, inducing regional changes in other important intracellular ions.  (+info)

Augmentation of L-type calcium current by hypoxia in rabbit carotid body glomus cells: evidence for a PKC-sensitive pathway. (3/141)

Previous studies have suggested that voltage-gated Ca(2+) influx in glomus cells plays a critical role in sensory transduction at the carotid body chemoreceptors. The purpose of the present study was to determine the effects of hypoxia on the Ca(2+) current in glomus cells and to elucidate the underlying mechanism(s). Experiments were performed on freshly dissociated glomus cells from rabbit carotid bodies. Ca(2+) current was monitored using the whole cell configuration of the patch-clamp technique, with Ba(2+) as the charge carrier. Hypoxia (pO(2) = 40 mmHg) augmented the Ca(2+) current by 24 +/- 3% (n = 42, at 0 mV) in a voltage-independent manner. This effect was seen in a CO(2)/HCO(3)(-)-, but not in a HEPES-buffered extracellular solution at pH 7.4 (n = 6). When the pH of a HEPES-buffered extracellular solution was lowered from 7.4 to 7. 0, hypoxia augmented the Ca(2+) current by 20 +/- 5% (n = 4, at 0 mV). Nisoldipine, an L-type Ca(2+) channel blocker (2 microM, n = 6), prevented, whereas, omega-conotoxin MVIIC (2 microM, n = 6), an inhibitor of N and P/Q type Ca(2+) channels, did not prevent augmentation of the Ca(2+) current by hypoxia, implying that low oxygen affects L-type Ca(2+) channels in glomus cells. Protein kinase C (PKC) inhibitors, staurosporine (100 nM, n = 6) and bisindolylmaleimide (2 microM, n = 8, at 0 mV), prevented, whereas, a protein kinase A inhibitor (4 nM PKAi, n = 10) did not prevent the hypoxia-induced increase of the Ca(2+) current. Phorbol 12-myristate 13-acetate (PMA, 100 nM), a PKC activator, augmented the Ca(2+) current by 20 +/- 3% (n = 8, at 0 mV). In glomus cells treated with PMA overnight (100 nM), hypoxia did not augment the Ca(2+) current (-3 + 4%, n = 5, at 0 mV). Immunocytochemical analysis revealed PKCdelta-like immunoreactivity in the cytosol of the glomus cells. Following hypoxia (6% O(2) for 5 min), PKCdelta-like immunoreactivity translocated to the plasma membrane in 87 +/- 3% of the cells, indicating PKC activation. These results demonstrate that hypoxia augments Ca(2+) current through L-type Ca(2+) channels via a PKC-sensitive mechanism.  (+info)

Pore block versus intrinsic gating in the mechanism of inward rectification in strongly rectifying IRK1 channels. (4/141)

The IRK1 channel is inhibited by intracellular cations such as Mg(2+) and polyamines in a voltage-dependent manner, which renders its I-V curve strongly inwardly rectifying. However, even in excised patches exhaustively perfused with a commonly used artificial intracellular solution nominally free of Mg(2+) and polyamines, the macroscopic I-V curve of the channels displays modest rectification. This observation forms the basis of a hypothesis, alternative to the pore-blocking hypothesis, that inward rectification reflects the enhancement of intrinsic channel gating by intracellular cations. We find, however, that residual rectification is caused primarily by the commonly used pH buffer HEPES and/or some accompanying impurity. Therefore, inward rectification in the strong rectifier IRK1, as in the weak rectifier ROMK1, can be accounted for by voltage-dependent block of its ion conduction pore by intracellular cations.  (+info)

Contributions of protein disulfide isomerase domains to its chaperone activity. (5/141)

Protein disulfide isomerase (PDI), a member of the thioredoxin (Trx) superfamily, consists of five consecutive domains, a-b-b'-a'-c. Domain combinations, AB, A'C, B'A'C and AB-C, and hybrids of PDI domains with Trx, Trx-C and Trx-B'A'C, have been constructed and expressed in Escherichia coli to examine the contributions of PDI domains to its enzyme and chaperone activities. All the combination and hybrid products are considerably less active than intact PDI in their enzyme activities. Recombinant products containing C, at low concentrations, inhibit the reactivation of lysozyme in HEPES buffer, while those without C do not. Only the intact PDI molecule and the hybrid molecule, Trx-B'A'C, but to a much lower level, show general chaperone activity in assisting the reactivation of denatured D-glyceraldehyde-3-phosphate dehydrogenase. It is suggested that all domains of PDI contribute to the binding of target protein for its chaperone activity.  (+info)

Mechanisms of endothelial cell swelling from lactacidosis studied in vitro. (6/141)

One of the early sequelae of ischemia is an increase of circulating lactic acid that occurs in response to anaerobic metabolism. The purpose of the present study was to investigate whether lactic acidosis can induce endothelial swelling in vitro under closely controlled extracellular conditions. Cell volume of suspended cultured bovine aortic endothelial cells was measured by use of an advanced Coulter technique employing the "pulse area analysis" signal-processing technique (CASY1). The isosmotic reduction of pH from 7.4 to 6.8 had no effect on cell volume. Lowering of pH to 6.6, 6.4, or 6.0, however, led to significant, pH-dependent increases of cell volume. Swelling was more pronounced in bicarbonate-buffered media than in HEPES buffer. Specific inhibition of Na(+)/H(+) exchange by ethylisopropylamiloride completely prevented swelling in HEPES-buffered media. Pretreatment with ouabain to partially depolarize the cells did not affect the degree of acidosis-induced swelling. In bicarbonate-buffered media, the inhibition of transmembrane HCO(3)(-) transport by DIDS reduced swelling to a level comparable with that seen in the absence of bicarbonate ions. Lactacidosis-induced endothelial swelling, therefore, is a result of intracellular pH regulatory mechanisms, namely, Na(+)/H(+) exchange and bicarbonate-transporting carriers.  (+info)

Regulation of the epithelial Na(+) channel by extracellular acidification. (7/141)

The effect of extracellular acidification was tested on the native epithelial Na(+) channel (ENaC) in A6 epithelia and on the cloned ENaC expressed in Xenopus oocytes. Channel activity was determined utilizing blocker-induced fluctuation analysis in A6 epithelia and dual electrode voltage clamp in oocytes. In A6 cells, a decrease of extracellular pH (pH(o)) from 7.4 to 6.4 caused a slow stimulation of the amiloride-sensitive short-circuit current (I(Na)) by 68.4 +/- 11% (n = 9) at 60 min. This increase of I(Na) was attributed to an increase of open channel and total channel (N(T)) densities. Similar changes were observed with pH(o) 5.4. The effects of pH(o) were blocked by buffering intracellular Ca(2+) with 5 microM 1, 2-bis(2-aminophenoxy)ethane-N,N,N',N'-tetraacetic acid. In oocytes, pH(o) 6.4 elicited a small transient increase of the slope conductance of the cloned ENaC (11.4 +/- 2.2% at 2 min) followed by a decrease to 83.7 +/- 11.7% of control at 60 min (n = 6). Thus small decreases of pH(o) stimulate the native ENaC by increasing N(T) but do not appreciably affect ENaC expressed in Xenopus oocytes. These effects are distinct from those observed with decreasing intracellular pH with permeant buffers that are known to inhibit ENaC.  (+info)

Binding of dystrophin's tandem calponin homology domain to F-actin is modulated by actin's structure. (8/141)

Dystrophin has been shown to be associated in cells with actin bundles. Dys-246, an N-terminal recombinant protein encoding the first 246 residues of dystrophin, includes two calponin-homology (CH) domains, and is similar to a large class of F-actin cross-linking proteins including alpha-actinin, fimbrin, and spectrin. It has been shown that expression or microinjection of amino-terminal fragments of dystrophin or the closely related utrophin resulted in the localization of these protein domains to actin bundles. However, in vitro studies have failed to detect any bundling of actin by either intact dystrophin or Dys-246. We show here that the structure of F-actin can be modulated so that there are two modes of Dys-246 binding, from bundling actin filaments to only binding to single filaments. The changes in F-actin structure that allow Dys-246 to bundle filaments are induced by covalent modification of Cys-374, proteolytic cleavage of F-actin's C-terminus, mutation of yeast actin's N-terminus, and different buffers. The present results suggest that F-actin's structural state can have a large influence on the nature of actin's interaction with other proteins, and these different states need to be considered when conducting in vitro assays.  (+info)

HEPES is a zwitterionic organic chemical buffering agent. HEPES is commonly added to media at concentrations ranging from 10 mM to 25 mM. Concentrations greater than 50 mM are not recommended since it can result in cell toxicity. Depending on your application the use of HEPES with Cu(II) should be carefully considered as a possible interaction may occur1.. Recently, HEPES has found an increasing role outside the area of biochemisty such as in the field of nanoparticles2,3,4,5.. Buffering pH range 6.8 - 8.2.. Free Shipping within the Continental USA. ...
Abstract: We have developed a new standardized method for the post-embedding immunoelectron microscopy using the same fixation, antigen retrieval and image contrasting procedures. Tissues were fixed with 4% formaldehyde containing 2.5 mM CaCl2, 1.25 mM MgCl2 in a 0.1 M 4-(2-hydroxyethyl)-piperazineethanesulfonic acid (HEPES) buffer (pH 7.4) for 2 h and then with the same fixative composition in 0.1 M HEPES buffer (pH 8.5) overnight at room temperature. Vehicle osmolarity of fixatives was adjusted to 300-330 mOsm by adding glucose. The specimens were dehydrated with dimethylformamide on ice and embedded in LR-White resin. Ultrathin sections were heated in a 20 mM Tris-HCl buffer (pH 9.0) for 1-2 h at 95°C. After immuno-gold labeling, the sections were treated with 2% glutaraldehyde containing 0.05% tannic acid in a 0.1 M phosphate buffer (pH 5.5) for 5 min and with a 1% OsO4/0.1 M phosphate buffer (pH 7.4) for 5 min, and then they were double stained with uranyl acetate and lead citrate. The ...
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HEPES (Fine White Crystals/Molecular Biology), Fisher BioReagents Poly Bottle; 500g HEPES (Fine White Crystals/Molecular Biology), Fisher BioReagents Buffering Agents
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Insulin solution from bovine pancreas (insulin 10mg/mL 25 mM HEPES, pH 8.2, BioReagent, sterile-filtered, cell culture tested); Suitable for mammalian cell culture; Recommended for use in cell culture applications at 0; Two-chain polypeptide hormone produced by the β-cells of pancreatic
TRAPP was purified from 300 OD599 units of cells. SFNY904 (MATα ura3-52 bet3Δ::URA3 leu2-3, 112 BET3-protein A::LEU2 L-A-o) was used for the purification because it contained protein A-tagged Bet3p and was free of the L-A virus (L-A-o). The L-A virus coat protein, gag, is a common contaminant in the purification (see Sacher et al. 2000). To purify TRAPP, cells were converted to spheroplasts, lysed in 3 ml of buffer B (150 mM KCl, 20 mM Hepes, pH 7.2, 2 mM EDTA, 1% Trition X-100, 0.5 mM DTT, protease inhibitor cocktail), and the unbroken cells were removed as described above. The lysate was centrifuged at 14,000 g for 10 min and the supernatant (10 mg/ml of protein) was incubated with 150 μl of a 50% slurry of IgG-Sepharose (Amersham Pharmacia Biotech) for 4 h. The beads were washed three times with 3 ml of release buffer (20 mM Hepes, pH 7.2, 5 mM MgCl2, 1 mM DTT, 0.75 mM GTP, 0.75 mMGDP, 1 mg/ml BSA) or uptake buffer (20 mM Hepes, pH 7.2, 5 mM MgCl2, 1 mM EDTA, 1 mM ATP, 1 mM DTT, 1 mg/ml ...
Expansion of BMSCs: Bone marrow aspirate collections containing 8 x 107 MNCs were seeded within each 150-cm2 tissue culture flask. Culture medium composed of alpha-minimal essential medium (α-MEM) supplemented with 10% v/v heat-inactivated fetal bovine serum (FBS), penicillinstreptomycin-glutamine, 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES), and sodium pyruvate (all from Life Technologies, Burlington, Canada) was pipetted into each flask. Fibroblast growth factor-2 (FGF-2; Neuromics Inc., Edina, USA) was added at a concentration of 5 ng/ml in order to maintain cell multipotency. Nucleated cells were allowed to adhere and grow for seven days before the first media change under normoxia (ambient 21% O2) or hypoxia (low 3% O2) at 37°C in a humidified incubator containing 5% CO2. Flasks from the hypoxic incubator experienced short periods (less than 5 minutes) of normoxic exposure during media changes. Thereafter, the media were changed twice per week until 80% cell confluence was ...
Background: Some respiratory diseases may induce alveolar hypoxia thereby hypoxic pulmonary vasoconstriction (HPV). This study was the first to investigate the role of anion exchanger in sustained HPV.. Methods: Experiments were performed in the isolated perfused rabbit lung. In the HCO3- free groups, HEPES were added to the perfusate instead of bicarbonate. In the HEPES1 and HEPES2 groups, the lungs were ventilated with hypoxic gas with or without CO2 respectively.. Results: Ventilation the lungs with hypoxic gas resulted in biphasic HPV. No alteration in both phases of HPV was detected by DIDS (anion exchanger inhibitor,200 microM). However, DIDS (400 microM), extended ascending part of acute HPV until min 24. Both phases of HPV were decreased in the HEPES1 group. But in the HEPES 2 group, HPV tended to increase significantly during rising part of acute phase with no change during sustained phase.. Conclusions: This study is suggesting that anion exchanger may modulate HPV especially during ...
Samples were then centrifuged at 100 000 g at 4°C for 1 hour and the pellet containing OMPs was washed with 3 ml of 10 mM HEPES buffer. After final centrifugation at 100 000 g at 4°C for 1 hour the pellet was suspended in 100 μl of 10 mM HEPES ICG-001 purchase buffer. Protein concentration was measured using the Bradford assay. Two to four independent OMP preparations were made from each strain grown in particular conditions. Identification of OprE by LC-MS/MS analysis OM proteins were resolved by SDS-PAGE and visualized. by Coomassie Blue staining. The band of interest was excised from the gel and in-gel digested with modified sequencing grade trypsin (Promega), as in [36]. Peptides from in-gel-digested samples were purified with StageTips [37] and analyzed by LC-MS/MS using an Agilent 1200 AZD6244 nmr series nanoflow system (Agilent Technologies, Santa Clara, CA) connected to a LTQ. Orbitrap classic mass spectrometer (Thermo Electron, Bremen, Germany) that was equipped with a ...
Growth of the mouse mammary epithelial cell line designated COMMA-D has been studied in serum-free medium (SFM) formulated with Hams F12 and Dulbeccos modified Eagles medium (1/1) containing 15 mm 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid, 2 mm glutamine, gentamicin (50 µg/ml; basal medium) and supplemented with insulin (10 µg/ml), transferrin (10 µg/ml), selenous acid (10 ng/ml), epidermal growth factor (20 ng/ml; EGF), 10 nm 3,5,3′-triiodothyronine, 50 µm ethanolamine, 1.0 nm 17β-estradiol, 65 µm glutathione, and ovalbumin (100 µg/ml). COMMA-D cells were able to undergo serial passage and continued to exhibit dome formation after 20 passages in SFM. Cells seeded at low density in SFM underwent four population doublings at low passage number in 1 week compared to six doublings for cells grown in medium containing insulin, transferrin, selenium, EGF, and 1% fetal bovine serum. After many passages in SFM, the growth rates of cells were similar to those in serum-supplemented ...
This study describes the use of a microperfusion system to create rapid, large regional changes in intracellular pH (pH(i)) within single ventricular myocytes. The spatial distribution of pH(i) in single myocytes was measured with seminaphthorhodafluor-1 fluorescence using confocal imaging. Changes in pH(i) were induced by local external application of NH(4)Cl, CO(2), or sodium propionate. Local application was achieved by simultaneously directing two parallel square microstreams, each 275 microm wide, over a single myocyte oriented perpendicular to the direction of flow. One stream contained the control solution, and the other contained a weak acid or base. End-to-end, stable pH(i) gradients as large as 1 pH unit were readily created with this technique. This result indicates that pH within a single cardiac cell may not always be spatially uniform, particularly when weak acid or base gradients are present, which can occur, for example, in regional myocardial ischemia. The microperfusion method should
Macroscopic voltage‐gated sodium current (INa) was measured 24 hours after transfection with the standard whole‐cell patch clamp method at 22°C in both HEK293 cells and iPS‐CMs under both normal (pH 7.4) and moderate acidosis (extracellular and intracellular pH 7.0) conditions. For HEK293 cells, the pipette solution contained (in mmol/L) EGTA 2, CsF 120, CsCl2 20, HEPES 5, and NaCl 5 and was adjusted to pH 7.4 or 7.0 with CsOH; the bath solution contained (in mmol/L) CaCl2 1.8, NaCl 140, HEPES 5, MgCl2 0.75, and KCl 4 and was adjusted to pH 7.4 or 7.0 with NaOH.20, 21 For iPS‐CMs, the pipette solution contained (in mmol/L) HEPES 10, NaCl 5, CaCl2 2, CsCl2 135, MgATP 5, and EGTA 10 and was adjusted to pH 7.4 or 7.0 with CsOH; the bath solution contained (in mmol/L) CsCl2 105, NaCl 60, glucose 10, CaCl2 1.8, MgCl2 1, HEPES 5, and Nifedipine 0.001 and was adjusted to pH 7.4 or 7.0 with CsOH (modified from reference).22 The resistances of microelectrodes ranged from 1.0 to 2.3 MΩ. Voltage ...
This study discusses the effect of key factors like containers, buffers and the freeze (controlled vs. flash freezing) and thawing processes on the stability of a therapeutic protein fibroblast growth factor 20 (FGF-20). The freezing profiles monitored by 15 temperature probes located at different regions in a 2-L bottle during freezing can be grouped into three categories. A rapid drop in temperature was observed at the bottom followed by the top and middle center of the bottle. The freeze-thawing behavior in a 50 ml tube is considerably uniform, as expected. Among phosphate, HEPES (4-(2-hydroxyethyl)-1-piperazine ethanesulfonic acid), citrate and histidine (each containing 0.5 M arginine-sulfate) buffer systems, a minimum pH change (0.4 pH unit vs. ∼1.7 pH unit) was observed for the phosphate buffer system. Thawing in a 50 ml tube at room temperature standing resulted in a significant phase separation in citrate, histidine and HEPES buffers; however, phase separation was least in the ...
He catalytic activity; replacement of HEPES/KOH buffer with TRIS/HCl abolished the enzymatic activity unless a monovalent cation was present (Figure 3C). Among
|p||strong|Technical Advantage: |/strong| Lower documented Endotoxin content then offerings from other major suppliers|br /| Iscoves Modification of DMEM (IMDM) is highly enriched synthetic medium optimized for rapidly proliferating cultures of high cell density.  A modified form of Dulbeccos Modified Eagle Medium (DMEM), Iscoves contains additional amino acids, vitamins, selenium and HEPES buffer, and is formulated with potassium nitrate in place of ferric nitrate. The addition of albumin and transferrin may be required for certain cell types.  This formulation contains 25 mM HEPES, and does not contain L-glutamine, alpha-thioglycerol and beta-mercaptoethanol.|br /| -  Complete Certificate of Analysis available for each production lot, along with full formulation information|br /| -  Produced under the highest industry standards to ensure superior results|/p|
MEM, originally prepared by Harry Eagle, is one of the most popular cell culture media. Upon his attempts to cultivate normal mammalian fibroblasts and certain HeLa cell subtypes, it was revealed that the nutritional needs of these cell types could not be met by BME. Further studies led to the development of MEM incorporating specific modifications such as higher amino acid concentrations for the cultivation of fastidious cells. MPs MEM may be used to support the growth of cells in monolayers, in suspension and wide variety of other cell types with proper supplementation. MP offers MEM with either Earles or Hanks salts.
7.5 × 106 l(2)mbn cells were pelleted, washed once in PBS, and resuspended in 800 μl of buffer A (10 mM Hepes, pH 7.6, 10 mM KCl, 1.5 mM MgCl2, 0.1 mM EDTA, 0.1 mM EGTA, Complete™ protease inhibitors; Roche), and placed on ice for 15 min. NP40 was added to 0.1%, vortexed for 30 s, and centrifuged at 13 K for 30 s at 4°C. Nuclear pellet was resuspended in 80 μl buffer C (10mM Hepes, pH 7.6, 400 mM NaCl, 7.5 mM MgCl2, 0.2 mM EDTA, 0.1 mM EGTA, 1 mM DTT, 0.5 mM PMSF, Complete™), and incubated on ice for 40 min while shaking. Extract was then centrifuged for 5 min at 13 K and supernatant aliquoted and frozen at -70° C. Nuclear extracts from second instar, wandering third instar, early pupae, and larvae specifically staged at -18 and -4 h (relative to puparium formation) from wild-type and rbp5 larvae were prepared as follows. Extracts were made by homogenizing 50 larvae in 300 μl of buffer A and removing them to a fresh tube in a total of 600 μl devoid of larval debri. After incubation on ...
To determine selectivity against αIIbβIIIa, wells of a 96-well Immulon-2 microtiter plate were coated with 10 μg/ml RGD affinity-purified human αIIbβIIIa in 10 mM HEPES, 150 mM NaCl, and 1 mM MgCl2, pH 7.4 (500 ng/well αIIbβIIIa final), and then the pate was incubated overnight at 4°C. The next day, the wells were blocked with 5% BSA in the above-mentioned buffer at room temperature for 2 h. The assay plate was washed five times with modified Tyrodes buffer (150 mM NaCl, 12 mM NaHCO3, 2.6 mM KCl, 2.5 mM HEPES, 1 mM MgCl2, and 1 mg/ml BSA, pH 7.4). Biotinylated fibrinogen was prepared using human fibrinogen according to directions from the biotin-X-NHS biotinylation kit. Fifty microliters of the compound/biotinylated fibrinogen mix was transferred to the assay plate and incubated at room temperature for 2 h. After incubation, the assay plate was washed five times with modified Tyrodes buffer. Reactions were visualized as described above. JNJ-26076713 was tested at half-log doses from ...
KD=7 nM. Caliper IC50 assay. The assay was performed using 384-well microtiter plates. Compounds were tested as 8-point dose responses. The assays were prepared by addition of 50 nL of compound solution in 90% DMSO directly into the empty plate. Subsequently, 4.5 μL of the enzyme solution (50 mM HEPES pH 7.5, 1 mM DTT, 0.02% Tween20, 0.02% BSA, 10 mM beta-glycerolphosphate, 10 μM sodium orthovanadate, 3 mM MgCl2 and 0.6 nM PAK1 (249-545) wt nonphos, produced in-house by expression in E. coli cells and purified by affinity chromatography) was added to each well and the resulting solution was pre-incubated at 30°C for 60 min, followed by addition of 4.5 μL of the peptide/ATP solution (50 mM HEPES pH 7.5, 1 mM DTT, 0.02% Tween20, 0.02% BSA, 10 mM beta-glycerolphosphate, 10 μM sodium orthovanadate, 3 mM MgCl2, 400 μM ATP and 4 μM peptide (5-Fluo-Ahx-AKRRRLSSLRA-COOH, Biosyntan GmbH). After 60 min incubation at 30°C, reactions were terminated by addition of 16 μL per well of the stop ...
IMDM - ISCOVES MODIFIED DULBECCOS MEDIUM. In 1976, Guilbert and Iscove demonstrated that precursor cells of erythrocytes and macrophages could be cultured in a reduced-serum medium supplemented with albumin, transferrin, lecithin, and selenium.. Iscoves Medium is a variation of Dulbeccos Modified Eagles Medium (DMEM) containing:. ...
Normales humanes Citratplasma wird durch Immunadsorption depletiert. Ein die Aktivität des depletierten Gerinnungsfaktors inhibierender Antikörper wird hinzugeben. Die Inhibitorplasmen sind sowohl lyophilisiert als auch gefroren verfügbar. Das lyophilisierte Plasma ist mit 50 mM HEPES gepuffert und enthält Stabilisatoren, das gefrorene Plasma ist mit 20 mM HEPES gepuffert ...
The increased steady state myocardial pHi in the SHR in nominally bicarbonate-free buffer provides evidence for hyperactivity of the Na+-H+ exchanger in the hypertensive and/or hypertrophic myocardium. This evidence was further strengthened by the following findings: (1) the faster recovery from the intracellular acid load induced by switching from HEPES to CO2/HCO3− buffer and the suppression of this difference when the Na+-H+ exchanger was inhibited, (2) the greater effect of blocking the Na+-H+ exchanger with EIPA in SHR myocardium exposed to HEPES-buffer, and (3) the lack of effect of the disulfonic derivative SITS on resting pHi in SHR myocardium in HEPES buffer bubbled with oxygen, ruling out a possible role of alkalinizing bicarbonate-dependent mechanisms.32 A still unanswered question in the present study is whether the hyperactivity of the Na+-H+ exchanger that we detected is a characteristic of the hypertensive animals or of the hypertrophic myocardium itself. Further experiments are ...
0.1M ammonium sulfate, 24-32% PEG5000 monomethyl ether, 1mM ATP, 100mM Hepes buffer, pH 7.2, VAPOR DIFFUSION, SITTING DROP, temperature ...
The vascular effect of peroxynitrite (ONOO-), a product of superoxide anion and nitric oxide, in isolated canine coronary arteries bathed in a 10 mM 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid-buffered physiological salt solution (pH 7.4) was investigated. ONOO- was synthesized from nitrite and H2O2 in a quenched-flow reactor. Addition of 0.01 to 30 microM ONOO- produced a rapid, dose-dependent relaxation in all 17 rings of coronary arteries with a threshold concentration of 0.1 microM, IC50 of 1.0 +/- 0.1 microM and Emax of -96 +/- 0.5% (Means +/- S.E.M.). Incubation of arteries in a standard bicarbonate-buffered Krebs-Henseleit solution decreased slightly the sensitivity of ONOO- relaxation but did not alter the maximum effect (Emax = -97 +/- 1.1, n = 6 vessels). The ONOO(-)-induced coronary relaxation was reversible upon washing, and was also reproducible with repeated testings in the same ring. Mechanical removal of intimal endothelium did not alter the observed relaxant effect. ...
With HEPES buffer, L-glutamine, and phenol red. Without sodium bicarbonate. Store at 2˚ to 8˚C.. Find this and many more products.
A simple fluorophore bearing a diethylaminocoumarin donor and a pyridinium acceptor was synthesized and utilized for the ultra-sensitive detection of heparin. The synthesized dicationic push-pull coumarin derivative emits strongly in the red-region (665 nm) and detects nanomolar concentrations (14.8 nM to 148 nM) of heparin in HEPES buffer and FBS serum solutions. The dication exhibits excellent fluorescence selectivity and sensitivity towards heparin over its analogues such as chondroitin 4-sulfate (CS), hyaluronic acid (HA) and dextran. This fluorescence assay is a convenient, sensitive method for monitoring heparin levels in biological samples. These findings were confirmed using coarse-grained Monte Carlo simulations, which provide us with a rationale for the selective binding of heparin ...
Purpose: : The human RPE is a polar organized, highly specialized epithelium. To maintain its functions multiple interactions among the RPE-cells and the neighboring tissues are necessary and play an important role in the pathology of innummerous diseases of the posterior segment. This study aims to investigate whether a perfusion tissue culture of the human retina-RPE-choroidea complex could be a suitable model to study these diseases in vitro. Methods: : Human retina-RPE-choroidea-tissue was isolated from patients after enucleation because of various reasons. Written consent was obtained prior to surgery. After removal of the anterior segment and the vitreous the choroidea-RPE-retina complex was separated from the sclera and transferred immediately into a gradient tissue culture chamber (Minucells&Minutissue, Bad Abbach, Germany) kept at 37°C and perfused with DMEM and Hams F12 1:1, supplemented with 10% human serum, 25mM HEPES and 1% penicillin-streptomycin, 110mg/l sodium-pyruvat for five ...
After 1 d in culture, cells were continuously superfused (2 ml/min) with physiological solution containing the following (in mm): 152 NaCl, 5 KCl, 1 MgCl2, 2 CaCl2, 10 glucose, and 10 HEPES, pH adjusted to 7.4 with NaOH, as previously reported (Fabbretti et al., 2006). Cells were voltage clamped (whole-cell configuration) using pipettes filled with the following (in mm): 140 KCl, 0.5 CaCl2, 2 MgCl2, 2 Mg2ATP3, 2 GTP, 10 HEPES, and 10 EGTA, pH adjusted to 7.2 with KOH (at −60 mV, 70% series resistance compensation). Agonists were applied with a fast superfusion system (Rapid Solution Changer RSC-200; Bio-Logic, Claix, France) with solution exchange time (10-90%) of 30-40 ms. Responses to agonists were measured in terms of peak amplitude. To express agonist potency in terms of EC50 values (concentration producing 50% of the maximum response), dose-response curves for the selective P2X3 receptor agonist α,β-methyleneATP (α,β-meATP) were constructed by applying different agonist doses to the ...
Electrophysiology. Whole-cell recordings from acutely isolated rat striatal neurons were obtained using previously published techniques (Surmeier et al., 1995; Mermelstein et al., 1999). The pipette solution consisted of (in mm): 180N-methyl-d-glucamine (NMG), 40 HEPES, 4 MgCl2, 0-20 BAPTA, 12 phosphocreatine, 2 Na2ATP, 0.2 Na2GTP, and 0.1 leupeptin, pH 7.2-7.3 with H2SO4, 265-270 mOsm/l. The external solution consisted of (in mm): 135 NaCl, 20 CsCl, 1 MgCl2, 10 HEPES, 0.001 TTX, 5 BaCl2, and 10 glucose, pH 7.4 with NaOH, 300-305 mOsm/l. All reagents were obtained from Sigma (St. Louis, MO) except ATP and GTP (Boehringer Mannheim, Indianapolis, IN) and BAPTA, calcineurin autoinhibitory peptide, and leupeptin (Calbiochem, La Jolla, CA). All drugs were prepared according to the manufacturers specifications and applied with a sewer pipe capillary array (Surmeier et al., 1995; Mermelstein et al., 1999). C terminus of β adrenergic receptor kinase 1 (βARK-C) peptide (βARK-Cp) is comprised of ...
DMEM (Dulbeccos Modified Eagle Medium) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM include primary fibroblasts, neurons, glial cells, HUVECs, and smooth muscle cells, as well as cell lines such as HeLa, 293, Cos-7, a
InCHi String: isomeric and canonical SMILES: C1CN(CC[NH+]1CCO)CCS(=O)(=O)[O-]. IUPAC: IUPAC traditional: IUPAC cas: IUPAC openeye: IUPAC systematic ...
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If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the Pub Med ID of your paper to get a coupon. ...
Entecavir is used in the treatment of HIV Infection and Herpes Infection. BUY the tablets from Cheap Medicine Shop and save more on all your medicines.
Synonyms: HEPES sodium salt, or 4-(2-hydroxyethyl)piperazine-1-ethanesulfonic acid sodium salt, or N-(2-hydroxyethyl)piperazine-N-(2-ethanesulfonic acid) sodium salt ...
HDAC enzymatic assays:. Tubastatin A is dissolved and diluted in assay buffer (50 mM HEPES, pH 7.4, 100 mM KCl, 0.001% Tween-20, 0.05% BSA, and 20 μM tris(2-carboxyethyl)phosphine) to 6-fold of the final concentration. HDAC enzymes are diluted to 1.5-fold of the final concentration in assay buffer and pre-incubated with Tubastatin A for 10 minutes before the addition of the substrate. The amount of FTS (HDAC1, HDAC2, HDAC3, and HDAC6) or MAZ-1675 (HDAC4, HDAC5, HDAC7, HDAC8, and HDAC9) used for each enzyme is equal to the Michaelis constant (Km), as determined by a titration curve. FTS or MAZ-1675 is diluted in assay buffer to 6-fold the final concentration with 0.3 μM sequencing grade trypsin. The substrate/trypsin mix is added to the enzyme/compound mix and the plate is shaken for 60 seconds and then placed into a SpectraMax M5 microtiter plate reader. The enzymatic reaction is monitored for release of 7-amino-4-methoxy-coumarin over 30 minutes, after deacetylation of the lysine side chain ...
CM cell collection Version 3 University of Pennsylvania For Patients 14-17 Human cardiomyocyte dissociation. Myocardial tissue was washed and resuspended in cold isolation buffer (130 mM NaCl; 5 mM KCl; 1.2 mM KH2PO4; 6 mM HEPES; 5 mM NaHCO3; 1 mM MgCl2; 5 mM Glucose) pH 7.4. Isolation buffer was supplemented with 0.36mM CaCl2 for enzyme activity. Cardiac tissue was incubated for 15 -20mins in isolation buffer supplemented with Collagenase IV (5mg/ml stock ,Sigma). After each incubation step, the supernatants were transferred to a tube and centrifuged at 600 rpm for 4 min. Cell pellets were resuspended in ice-cold isolation buffer. Several rounds of digestion were performed until tissue was fully digested.. Flow Cytometry and FACS. Isolated cardiac cell suspensions obtained from human heart tissue were sorted on a FACSAria (Becton Dickenson Biosciences) instrument operating at low pressure (20 psi) using a 100µm nozzle. Initial size gates for forward scatter (FSC) vs. side scatter (SSC) were ...
CM cell collection Version 4 University of Pennsylvania For Patients 18-19 Date: 12/2014 Human cardiomyocyte dissociation. Myocardial tissue was washed and resuspended in cold isolation buffer (130 mM NaCl; 5 mM KCl; 1.2 mM KH2PO4; 6 mM HEPES; 5 mM NaHCO3; 1 mM MgCl2; 5 mM Glucose) pH 7.4. Isolation buffer was supplemented with 0.36mM CaCl2 for enzyme activity. Cardiac tissue was incubated for 15 -20mins in isolation buffer supplemented with Collagenase IV (5mg/ml stock ,Sigma). After each incubation step, the supernatants were transferred to a tube and centrifuged at 600 rpm for 4 min. Cell pellets were resuspended in ice-cold isolation buffer. Several rounds of digestion were performed until tissue was fully digested.. Flow Cytometry and FACS.. Isolated cardiac cell suspensions obtained from human heart tissue were sorted on a FACSAria (Becton Dickenson Biosciences) instrument operating at low pressure (20 psi) using a 100µm nozzle. Initial size gates for forward scatter (FSC) vs. side ...
Human platelet isolation. Blood was collected from three genetically unrelated donors that were free from nonsteroidal antiinflammatory drugs for at least 14 days. Blood was collected into acid-citrate-dextrose (ACD; 85 mM trisodium citrate, 65 mM citric acid, and 100 mM glucose) at a blood/ACD ratio of 8.1:1.9 (v/v) and centrifuged at 250 g for 10 minutes at room temperature (22°C). Platelet-rich plasma was collected and centrifuged at 900 g for 10 minutes, and the pellet resuspended in Tyrodes buffer (134 mM NaCl, 12 mM NaHCO3, 2.9 mM KCl, 0.34 mM Na2HPO4, 1.0 mM MgCl2, 10 mM HEPES, and 5 mM glucose, pH 7.4) containing ACD (9:1, v/v). Platelets were washed by centrifuging at 800 g for 10 minutes and then resuspended in Tyrodes buffer at a concentration of 2 × 108 cells/ml. Where activated, they were prewarmed for 1 min at 37°C, with 1 mM CaCl2, before addition of 0.2 U/ml thrombin for 30 min.. Lipid extraction. Lipids were extracted by adding a solvent mixture (1 M acetic ...
With L-glutamine, sodium pyruvate, and 15 mM HEPES. Without phenol red and sodium bicarbonate. Store at 2˚ to 8˚C.. Find this and many more products.
Prostate cells accumulate great cellular and mitochondrial concentrations of zinc, generally 3C10-collapse higher than additional mammalian cells. numerous Zn-Ligand preparations with log for 5 min. The supernatant fluid was centrifuged for 7 min at 12 000and the producing pellet was washed twice in isolation buffer comprising 0.25% fatty acid-free BSA, and washed once in reaction buffer (250 mM sucrose, 10 mM HEPES and 5 mM KH2PO4). The final mitochondrial pellets were suspended in reaction buffer and modified to provide a mitochondrial concentration around 20 mg protein ml?1. Protein assay was performed 148-82-3 supplier by the method of Bradford [11]. The condition of the mitochondrial preparations was checked by dedication of oxygen usage and respiratory control with the aid of a dietary fiber optic oxygen monitoring system. Preparations that did not meet the criteria 148-82-3 supplier of no detectable endogenous respiration and a succinate-stimulated respiratory control percentage 2.5 were ...
Patch pipettes, filled with a solution consisting of 140 mm KCl, 5 mm EGTA, 5 mm MgCl2, and 10 mm HEPES, pH 7.3, had resistances of 3-6 MΩ. An outside-out patch24 with a seal resistance of 5 GΩ or greater was excised from a cell and moved into position at the outflow of a HSSE-2 rapid perfusion device (ALA Scientific Instruments, Westbury, NY). The perfusion system consisted of solution reservoirs, manual switching valves, a solenoid-driven pinch valve, and two tubes inserted into the culture dish and had a time resolution of less than 100 μs.25 One tube contained extracellular solution without agonist (normal solution); the other contained extracellular solution with 300 μm acetylcholine (test solution). In the control protocol, the patch, initially perfused with normal solution, was exposed to a series of ten 0.25-s exposures to the test solution at 5-s intervals. Manual valves were used to connect to reservoirs containing a defined concentration of competitive antagonist with or without ...
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2-APB is a widely used chemical compound in ion channel research. It inhibits numerous channels that include inositol 1,4,5-trisphosphate receptors, store-operated calcium channels, connexins and TRP family cation channels TRPC3, TRPC5, TRPC6, TRPM2, TRPM3 and TRPM7. Native and overexpressed TRPM7 channels are inhibited by 2-APB with IC50-s in the 70-170 μM range. A characteristic of TRPM7 channels is their inhibition by intracellular Mg2+ and acidic pH. using patch-clamp electrophysiology, we recorded native TRPM7 channel activity in Jurkat T lymphocytes and tested 2-APB at 10-300 μM for its ability to inhibit TRPM7 currents. When internal HEPES buffer concentration was low (1 mM), 100-300 μM 2-APB inhibited 60-70% of TRPM7 current with a slow time course. This inhibition was voltage-independent and reversible. Increasing the pH buffering capacity of internal solution to 140 mM HEPES abolished the inhibitory 2-APB effect. Simply making the internal recording solution alkaline, greatly diminished 2
Crude synaptosomes (P2 fraction) were prepared from striatum, hippocampus, or cerebral cortex of young adult male Sprague-Dawley rats (150-200 g), from cerebral cortex of adult male C57BL/6 mice (25-30 g), or from cerebral cortex of adult male guinea pigs (400-500 g) as described. 26 Synaptosomes were suspended in HEPES buffered medium (composition: 140 mm NaCl, 5 mm KCl, 1 mm MgCl2, 1.2 mm Na2HPO4, 5 mm NaHCO3, 10 mm d-glucose, and 20 mm HEPES/NaOH, pH 7.4), collected by centrifugation at 8,000 g for 10 min, and stored on ice for up to 5 h until use. Release of endogenous glutamate was measured by an enzyme-linked fluorometric method. 27 Synaptosomal pellets (0.5 mg protein, determined by the method of Bradford 28) were resuspended in HEPES buffered medium plus 16 μm bovine serum albumin, 1 mm NADP+, 100 U l-glutamate dehydrogenase, and 1.3 mm CaCl2(1.5 ml final volume). Stirred samples were equilibrated at 37°C for 4 min in a spectrofluorometer cuvette, and data acquisition was initiated ...
TY - JOUR. T1 - Cation effects on cell shape.. AU - Sheetz, Michael. PY - 1977/12/1. Y1 - 1977/12/1. N2 - We have found that human erythrocyte ghosts in 10 mM HEPES (pH 7.0) at 0 degrees C would crenate when 20-50 mM of Na+ or K+, 0.2-0.5 mM OF Ca++, Ba++, Sr++, or Mg++, or 10 muM of La+++ was added. The shape change after cation addition was faster than fixation by 1% glutaraldehyde at 4 degrees C and was readily reversible upon dilution of the cation. After incubation of ghosts in 10 mM HEPES (pH 7.0) at 37 degrees for 10-20 min there was a significant inhibition of subsequent crenation by cations. In a process that is believed to occur by a similar mechanism, whole red blood cells were observed to cup (invaginate) when 20 mM of a divalent or 0.1 mM of a trivalent cation was added. After neuraminidase treatment to remove the sialic acid charge groups, these same shape changes were observed in ghosts and whole cells. Another type of cation-induced crenation was found to follow upon the addition ...
Lipoprotein-associated phospholipase A2 (Lp-PLA2) activity was measured by a modification of the trichloroacetic acid precipitation procedure in plasma using [3H]-PAF (1-O-hexadecyl-2-[3H-acetyl]-sn-glycero-3-phosphocholine) (10 Ci/mmol, DuPont-New England Nuclear, Boston, MA, USA) as a substrate at a final concentration of 100 μmol/L, as described previously[24]. Briefly, 50 μL diluted plasma (1∶25 v/v with HEPES pH7.4 buffer which consists of 4.2 mmol/L HEPES, 137 mmol/L NaCl, 2.6 mmol/L KCl, and 2 mmol/L EDTA) as the source of the enzyme or blanks (for determination of background) were mixed with assay buffer (HEPES) to a final volume of 90 μL. The reaction was allowed to proceed for 10 minutes at 37 °C by addition of 10 μL of working substrate solution (2 mmol/L [3Η]-PAF in 2.5×103 mg/L BSA, prepared fresh daily). The reaction was terminated by addition of 20 μL ice-cold aqueous bovine serum albumin (BSA) solution (100×103 mg/L) followed by vortex-mixing and incubation for 10 ...
Cell Culture. LNCaP.FGC 1740 cells frozen at passage 18 (American Type Culture Collection, Rockville, Maryland, USA) were routinely cultured in RPMI 1640 supplemented with 10% FBS, 15 mM HEPES buffer (pH 7.4), 2 mM L-glutamine and 100 μM nonessential amino acids (GIBCO BRL, Gaithersburg, Maryland, USA). LVCaP cells were routinely cultured in the LNCaP media supplemented with 50 μg/mL of Geneticin (GIBCO BRL).. The human melanoma cell lines, A875 and A875/E6, were kindly provided by M. Murphy (Fox Chase Cancer Center, Philadelphia, Pennsylvania, USA). The A875/E6 line harbors human papilloma virus (HPV)16E6. The human colon cancer cell lines RKO and RKO.mp53.13 were generous gifts from M. Kastan (St. Jude Childrens Research Hospital, Memphis, Tennessee, USA). RKO.mp53.13 was generated by transfecting parental RKO cells with a dominant-negative p53 mutant. Cells were maintained in a humidified atmosphere containing 5% CO2/95% air and were free of contamination with mycoplasma or ...
0069] Blockade of voltage-sensitive sodium channels was studied by investigating [3H] batrachotoxinin A 20-R-benzoate ([3H]BTX) displacement binding to whole brain membranes. Animals were decapitated and their brains quickly removed. Membrane preparation and binding assays were performed essentially as previously described (Shimidzu et al., 1997). Brains (without cerebellum) were homogenised in 10 vol 0.32 M sucrose, 1 mM EDTA, 1 mg/ml bovine serum albumin (BSA), 5 mM HEPES/TRIS pH 7.4 with a Teflon homogeniser (8 strokes at 400 r.p.m). After a 10 min centrifugation at 1,000 g the supernatants were centrifuged for 20 min at 39,000 g and pellets were homogenised with 20 vol or 40 vol Na+-free buffer, respectively for [3H]-batrachotoxinin A 20-alpha-benzoate ([3H]-BTX) binding assays. Na+-free buffer had the following composition (in mM): 130 choline chloride, 0.8 MgSO4, 5.4 KCl, 5.5 D-glucose, 50 HEPES/TRIS, pH 7.4. The homogenate was centrifuged for 20 min at 39,000 g and the resultant pellets ...
0110]Fibrin microthread preparation: Fibrin microthreads were co-extruded from solutions of fibrinogen and thrombin according to the schematic shown in FIG. 1. Fibrinogen from bovine plasma (Sigma, St. Louis, Mo., catalogue number F4753) was dissolved in HEPES Buffered Saline (HBS, 20 mM HEPES, 0.9% NaCl) at 70 mg/mL and stored at -20° C. Thrombin from bovine plasma (Sigma, St. Louis, Mo., catalogue number T4648) was stored frozen as a stock solution at a concentration of 40 U/mL in HBS. A working solution of thrombin was diluted from the stock to a final concentration of 6 U/mL in a 40 mM CaCl2 solution. Both the fibrinogen and thrombin solutions were warmed to 37° C. and placed into separate 1 mL syringes. The solutions were coextruded using a stabilized crosshead on a threaded rod with a crosshead speed of 4.25 mm/min through a blending applicator tip (Micromedics, Inc., St. Paul, Minn.). The blending applicators were Luer locked to the two syringes through individual bores and mixed in a ...
Chen medium: contains dextran, chondroitin sulfate, HEPES buffer, gentamicin, streptomycin sulfate, sodium carbonate, nonessential amino acids, vitamins; used for corneal storage
Originally, Hams Nutrient Mixtures were developed for the clonal growth of several Chinese hamster ovary (CHO) clone cells, Hela cells and mouse L-cells. Both F-10 and F-12 are formulated for use with or without serum, depending on the type of cells being cultured. F-10 has demonstrated satisfactory growth of human diploid cells, white blood cells for chromosomal analysis and primary rat, rabbit and chicken tissue explants. Hams F-12 is well suited for the growth of primary rat hepatocytes and prostate epithelial cells. It is also the medium of choice for toxicity assays using CHO cells. MP also offers Hams F-12 supplemented with 25 mM HEPES for improved buffering in the optimum pH range of 7.2-7.4. F12K is a modification of Hams F-12 and Coons F-12 with increased amino acid and pyruvate concentrations. Additionally, the salts have been modifed (Konig
The kinetic rate parameters for the interaction between a single domain analogue of staphylococcal protein A (Z) and a mouse-IgG3 monoclonal antibody (MAb) were measured in Hepes buffer with different chemical additives. Five buffer ingredients (pH, NaCl, DMSO, EDTA, and KSCN) were varied simultaneously in 16 experiments following a statistical experimental plan. The 16 buffers thus spanned a volume in chemical space. A mathematical model, using data from the buffer composition, was developed and used to predict apparent kinetic parameters in five new buffers within the spanned volume. Association and dissociation parameters were measured in the new buffers, and these agreed with the predicted values, indicating that the model was valid within the spanned volume. The pattern of variation of the kinetic parameters in relation to buffer composition was different for association and dissociation, such that pH influenced both association and dissociation and NaCl influenced only dissociation. This ...
CRYOcheck ™ Factor Deficient Plasma consist of pools of citrated human normal plasmas which have been depleted into a coagulation factor by immunoadsorption, buffered with HEPES buffer, aliquoted and frozen rapidly.. ...
DR is curious as to how the SureFire Optimzed Bolt Carrier/H7S Buffer combo stacks up against both the FERFRANS DSAS/RRS (Delayed Sear Activation System/Rate Reduction System) BCG and Nemo Arms RRS BCG, respectively. Weve been writing about the FERFRANS BCG for years, and were BIG fans of it. Both the OBC-LS/H7 Buffer System and DSAS/RRS BCG essentially achieve a similar effect through two completely different designs. Where the SureFire OBC-LS/H7 Buffer System utilizes a springloaded weight in the back of the carrier and teams the BCG up with a proprietary buffer, the FERFRANS DSAS/RRS BCG utilizes a simple reciprocating metal piece that recipricates freely within the BCG, via inertia. The the OBC-LS/H7 Buffer System is a more sophisticated design than the FERFRANS DSAS/RRS BCG, so it will be interesting to see how they compare back-to-back at some point.. DR has only examined and written about the Nemo Arms RRS BCG once when it was pulled out of a Nemo Arms Omen Recon .300WM 18″ ...
Standard electrophysiological techniques were used for potential recording and current injection under current-clamp conditions (Erxleben et al., 1995). For two-electrode voltage-clamp experiments, an Axoclamp 2B (Axon Instruments Inc., Foster City, CA) was used. These experiments were performed on fibers of the last abdominal segment which are compact (270-450 μm long with a diameter of 50-80 μm). Injection of current in the middle of the fibers and recording at several points over the length of the fiber showed that they are isopotential and thus suitable for two-electrode voltage-clamp. For isolation of Ca or Ba currents and suppression of K currents, a solution (TEA solution) consisting of 160 mM tetraethyl-ammonium, 2 mM 4-aminopyridine (4-AP), 320 mM NaCl, 8 mM KCl, 20 mM HEPES, pH 7.4, was used with either 10 mM CaCl2 or BaCl2. In addition, the recording and current electrodes were filled with 3 M CsCl to suppress K currents. Voltage-activated Ca or Ba currents were separated from ...
For typical Western blotting experiments, (e.g., Fig. 2J-L), cells were plated at 200,000 cells per well of a 12-well plate in complete Dulbeccos modified Eagles medium (DMEM)/F12 media containing 10% serum. The next day, cells were treated as indicated. For Western blotting involving production of cytokines, ARPE-19 cells were plated as described above followed by gradual serum removal over the course of a week until the serum was removed completely followed by indicated treatments. At appropriate time points, ARPE-19 cells were lysed in-plate with either radioimmunoprecipitation assay (RIPA) buffer (50 mM Tris, pH 7.4, 150 mM NaCl2, 1% Triton X-100 [vol/vol], 0.1% SDS [wt/vol], 0.5% sodium deoxycholate [wt/vol]) supplemented with Halt Protease and Phosphatase inhibitors (Pierce, Rockford, IL, USA) and benzonase (Sigma-Aldrich Corp.) or a buffer containing 50 mM HEPES pH 8, 10 mM KCl, 2 mM MgCl2, and 1.0% SDS also supplemented with benzonase. Cells were lysed at RT for approximately 1 to 2 ...
In isoflurane-anesthetized rats, 10% NaCl (total volume 300 μl) was injected subcutaneously on the medial aspect of the foot and medial aspect of the rat lower limb, which is innervated by the saphenous nerve. After 30 min, an in vitro skin-nerve preparation was dissected and used to record from single primary afferents in microdissected teased filaments of the saphenous nerve, as previously described.18The skin-nerve preparation was perfused at 15 ml/min with oxygen-saturated synthetic interstitial fluid buffer containing 123 mM NaCl, 3.5 mM KCl, 0.7 mM MgSO4, 1.7 mM NaH2PO4, 2.0 mM CaCl2, 9.5 mM sodium gluconate, 5.5 mM glucose, 7.5 mM sucrose, and 10 mM HEPES at pH 7.4. The mechanical receptive fields of individual units were identified by manually probing the skin with a glass rod. Αδ- and C-fiber mechanonociceptors were identified by their conduction velocity and threshold to mechanical stimulation by von Frey hairs, as described previously.3Stock solutions of DAMGO and CTOP were diluted ...
In order to obtain peritoneal macrophages, Balb/C mouse strain males, age 8 to 12 weeks, were injected i.p. with 300 fig of zymosan (SIGMA) dissolved in a phosphate buffer (PBS) in a total volume of 0.1 ml/mouse. After 24 hours the mice were euthanized according to the Laboratory Animal Welfare Act. The peritoneal cavity was washed with a sterile physiological solution (5 ml). The obtained peritoneal macrophages were washed twice with a sterile physiological solution and, after the last centrifugation (350 g/10 min), resuspended in RPMI 1640, into which 10% of FBS were added. In order to determine TNF-a secretion, SxlO4 cells/well were cultivated in a total volume of 200 \x\ for 18 to 24 hours on midrotitre plates with a flat bottom (96 wells. Falcon) in RPMI 1640 medium, into which 10% FBS (Fetal Bovine Serum, Biowhittaker) inactivated by heat, 100 units/ml of penicillin, 100 mg/ml of streptomycin, 20 mM HEPES and 50 μM 2-mercaptoethanol (all of GIBCO) were added. The cells were incubated at ...
The functional characterization of methaqualone at wild-type (WT) and mutant GABAARs expressed in Xenopus oocytes was performed essentially as previously described (Hoestgaard-Jensen et al., 2013). The GABAAR cDNAs were linearized and applied as templates for in vitro cRNA synthesis using the T7 mMESSAGE mMACHINE High Yield Capped RNA transcription kit (Life Technologies Corp., Carlsbad, CA. Either 9 or 18 nl cRNA encoding for α1β2 (α1:β2 ratio: 0.06:0.06 µg/µl) and α1,2,3,5β2,3γ2S GABAARs (α:β:γ2S ratio: 0.01:0.01:0.01 µg/μl), or 46 nl cRNA encoding for α6β2 (α6:β2 ratio: 1:0.1 µg/µl) and α4,6β1,2.3δ GABAARs (α:β:δ ratio: 1:0.1:1 µg/μl) were injected into oocytes, which subsequently were incubated at 18°C in modified Barths solution [88 mM NaCl, 1 mM KCl, 15 mM HEPES (pH 7.5), 2.4 mM NaHCO3, 0.41 mM CaCl2, 0.82 mM MgSO4, 0.3 mM Ca(NO3)2, 100 U/ml penicillin and 100 μg/ml streptomycin]. Whole-cell currents in the α1β2/α1,2,3,5β2,3γ2S- and ...
Animal studies. All animal experiments were conducted in the vivarium at Duke-NUS Medical School. C57BL/6 mice were purchased from InVivos. MC-deficient Sash mice (Wsh/Wsh) and γδ T cell-deficient mice (B6.129P2-Tcrdtm1Mom/J) were originally purchased from The Jackson Laboratory and bred in-house. Mcpt5-Cre/iDTR mice were generated by crossing Mcpt5-Cre mice (provided by Axel Roers, Dresden University, Dresden, Germany) with a Cre excision reporter strain, iDTR (C57BL/6-Gt(ROSA)26Sortm1(HBEGF)Awai/J), from The Jackson Laboratory. For all strains, 6- to 8-week-old female mice were used for the experiments.. Infections. For infections, Eden2, a clinical isolate of DENV2, was used. This low-passage clinical isolate was originally obtained from the Duke-NUS reference laboratory and derived from the Early Dengue Infection and Outcome (EDEN) clinical study (38). Viral strains were propagated in Aedes albopictus C6/36 mosquito cells (CRL-1660, ATCC), maintained in RPMI medium 1640 with 25 mM HEPES, ...
Structure, properties, spectra, suppliers and links for: 2-{[(3α,5β,6α,7α,17ξ)-3,6,7-Trihydroxy-24-oxocholan-24-yl]amino}ethanesulfonic acid.
Structure, properties, spectra, suppliers and links for: 2-{[(3α,5β,12α,17α,20S)-3,12-Dihydroxy-24-oxocholan-24-yl]amino}ethanesulfonic acid.
ERK1 Monoclonal Antibody from Invitrogen for Western Blot and ELISA applications. This antibody reacts with Human, Rat samples. Clone: 33A5. Supplied as 100 µL purified antibody in HEPES with 0.15M NaCl, 0.01% BSA, 50% glycerol and 0.03% sodium azide.
Dr. Addagada Rao answered: 2 to 7 days: Will take less than a wk to hepes simplex ii infection , once get infected stays in the body for ever , anti viral ...
A buffer is a mixture of molecules that release or bind H+ in order to maintain a relatively stable pH. Note that the function of a buffer is NOT to keep a solution neutral (at pH 7); its function is to minimize the change in pH when base or acid is added to the solution. Also note that there are many different buffers, and each one will stabilize the pH of a solution only within a specific pH range. One buffer may be effective within a range of pH 2 to pH 6, while another may be effective within a range of pH 10 to pH 12. Beyond its buffering range, a buffer no longer acts to stabilize the pH of the solution ...
I have a custom agenda view which shows the Items I did last week and the items I have to do in this week. This view is based on a filter of TODO items. However, it would be good to see scheduled items and deadlines in another buffer. Of course I could open another emacs instance and open there a different agenda, but is there another way so that I can examine two different agenda views within the same frame, but in different buffers?. ...
Stimulation by Hepes buffer". The Biochemical Journal. 254 (2): 519-23. doi:10.1042/bj2540519. PMC 1135108. PMID 3178771. K., ...
HEPES - NaOH 7.2 - 8.2 Additives[edit]. Salts[edit]. Lysis buffer usually contains one or more salts. The function of salts in ...
CAPSO CHES HEPES Good, N. E; Winget, G. D; Winter, W; Connolly, T. N; Izawa, S; Singh, R. M (1966). "Hydrogen ion buffers for ...
... when it was called Hepes, which in turn would produce Hiepes, Iepes and finally Yepes. It is possible that Hepes be a mozarabic ...
The activity of the enzyme is known to be inhibited by both Tris and HEPES buffers. Carbamoyl phosphate synthase (CPSase) is a ... by Tris and Hepes. Effect on Ka for N-acetylglutamate". Biochem. J. 243 (1): 273-276. doi:10.1042/bj2430273. PMC 1147843. PMID ...
13:33:09 (3, 2, 4) (EDIT) User:71.245.207.73 (contribs, talk) edited HEPES (diff, hist). Added links: http://www.labome.com/ ... review/HEPES.html. *. 13:36:57 (2, 4, 4) (EDIT) User:Beetstra (contribs, talk) edited Cysteine (diff, hist). Added: 'InChIKey ...
HEPES is a similar pH buffering compound that contains a piperazine ring. With a pKa of 7.20, MOPS is an excellent buffer for ...
For this reason, DEPC cannot be used with Tris or HEPES buffers. In contrast, it can be used with phosphate-buffered saline or ...
HEPES-buffered saline (HBS) (recipes from Dittmar, Liu, Ausubel etc.). *Gey's balanced salt solution (GBSS) ...
HEPES, POPSO and EPPS) can form radicals and should be avoided in studies of redox processes in biochemistry. Tricine is photo- ... "Hydrogen peroxide formation by reaction of peroxynitrite with HEPES and related tertiary amines. Implications for a general ...
1dg9: CRYSTAL STRUCTURE OF BOVINE LOW MOLECULAR WEIGHT PTPASE COMPLEXED WITH HEPES ...
Seems to have detrimental effects in embryo development in vitro; HEPES-buffered medium: used as buffered medium for human ... oocyte collection and embryo handling; MOPS-buffered medium: like HEPES, has the potential advantage that the buffering ...
MOPS HEPES MES Tris Common buffer compounds used in biology Good's buffers Good, Norman E.; Winget, G. Douglas; Winter, ...
The acidity of the solution must be regulated, often using a pH buffer such as HEPES. Isolation of cells from some tissues may ...
Negrete, Alejandro; Ling, Tau; Lyddiatt, Andrew (2008). "Effect of pluronic F-68, 5% CO2 atmosphere, HEPES, and Antibiotic- ...
HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid). 7.48. −0.014. 238.3 TES (2-[[1,3-dihydroxy-2-(hydroxymethyl)propan- ...
Hicks, M., and Gebicki, J. M., "Rate constants for reaction of hydroxyl radicals with Tris, Tricine, and Hepes buffers." "FEBS ...
MOPS HEPES MES Common buffer compounds used in biology Gomori, G., Preparation of Buffers for Use in Enzyme Studies. Methods ...
HEPES-buffered saline solution (HeBS) containing phosphate ions is combined with a calcium chloride solution containing the DNA ...
... contains: HEPES [pH 7.6], Urea, NaCl, DDT, PIC 1 & 2, 1.1% NP-40, Sodium orthovanadate, β-glycerol phosphate and ...
... and HEPES have very similar structures and properties, HEPBS also having an acidity (pKa) in the physiological range (7.6 ...
Retrieved 2012-09-10.CS1 maint: archived copy as title (link) CAPS (buffer) CHES MOPS HEPES HEPPS Tris Common buffer compounds ...
In order to get a meaningful sulfur signal from the analysis, the buffer should not contain sulfur (i.e. no BES, DDT, HEPES, ...
For example, in a study detecting tumor response to etoposide treatment, the sample was dissolved in 40 mM HEPES, 94 mM NaOH, ...
For example, application of 50 mmol/l HEPES or an equivalent of BUFFER ALL (ca. 1%) to IMDM (Iscove's Modified Dulbecco's ... 50 mmol/l HEPES) equilibrated against atmospheric air during a standard perfusion culture experiment shows constant partial ... a CO2 incubator has to be stabilized by reducing the NaHCO3 concentration and/or by adding biological buffers such as HEPES ( ...
... hepes MeSH D03.383.606.515 - hydroxyzine MeSH D03.383.606.515.200 - cetirizine MeSH D03.383.606.527 - 1-(5-isoquinolinesulfonyl ...
HEPES-buffered Saline (HBS) Hexagonal bilayer silica Hoboken Shore Railroad, a defunct railway in New Jersey, US Honmon ...
Buffer molecules can become part of the lattice (for example HEPES in becomes incorporated in crystals of human neutrophil ...
Hepes-glutamic acid buffer-mediated organic solvent protection effect (HOPE) gives formalin-like morphology, excellent ...
HEPES has the following characteristics: pKa1 (25 °C) = 3 pKa2 (25 °C) = 7.5 Useful pH range = 2.5 to 3.5 or 6.8 to 8.2 HEPES ... HEPES is widely used in cell culture, largely because it is better at maintaining physiological pH despite changes in carbon ... HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid) is a zwitterionic sulfonic acid buffering agent; one of the twenty ... Zigler JS, Lepe-Zuniga JL, Vistica B, Gery I (May 1985). "Analysis of the cytotoxic effects of light-exposed HEPES-containing ...
... Følgende præparat indeholder HEPES-buffer: Præparater, der ikke fremgår af listen, kan indeholde HEPES-buffer. ...
HEPES does not bind magnesium, calcium or manganese(II) ions2.. HEPES is not recommended for certain protein applications; it ... HEPES is a zwitterionic biological buffer and is one of Goods buffers. HEPES is better at maintaining physiological pH in cell ... HEPES sodium salt. 75277-39-3. C8H17N2NaO4S. 260.29. H7006 H3784 5380-OP. H8651. H3784. RES6007H-A7 H3662 ... HEPES (free acid). 7365-45-9. C8H18N2O4S. 238.30. RDD002. H3375. 5310-OP. 391338. 54457. H7523. H4034. H6147. PHG0001 RES6003H- ...
Studies have indicated that 20 mM HEPES is the most satisfactory concentration of the buffer when both Hanks and Earles ... HEPES (N-2-hydroxyethylpiperazine-N-2-ethane sulfonic acid) is a general purpose zwitterionic organic chemical buffering agent ... HEPES: 238300.00 mg/liter , Solution Product Families Description HEPES (N-2-hydroxyethylpiperazine-N-2-ethane sulfonic acid) ... HEPES buffer commonly used in cell culture media. The addition of 10-25 mM HEPES provides extra buffering capacity when cell ...
Sigma-Aldrich® HEPES Sodium Salt Physical Properties. Synonyms. 4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid sodium salt ...
Chart of resolution and migration profiles for Thermo Scientific Precise Protein Gels (Tris-HEPES Gels). Values in each lane ... Precise Tris-HEPES gel features *High-performance-unique running buffer helps produces excellent separation and high-resolution ... Precise Tris-HEPES gel selection table. Gel concentration. 10-well. 12-well. 15-well. ... In addition to Precise Tris-HEPES gels, we offer many other minigels in various formulations. Find the right one for your ...
HEPES Buffer Solution is a biological buffer for cell culture media. Selection of suitable nutrient medium depends on cell type ... Internal Search Keywords: HEPES buffer,HEPES solution,HIPS solution,HIPIS solution,HEEPS,7200 ... HEPES Buffer Solution is a biological buffer used in cell culture media. Selection of suitable nutrient medium is dependent on ...
Alfa Aesar HEPES Lysis Buffer with NP-40 250mL Life Sciences:Protein Biology:Protein Extraction and Purification:Protein ...
Der Ausfluss der Nukleotide, in isotonischem Cholinchlorid, wurde von Hepes nicht beeinflusst. ... Hepes Ghost Erythrocyte Ghost Reseal Erythrocyte Ghost Cholinchlorid This work was supported by the Cystic Fibrosis Research ... Efflux of cyclic AMP from resealed erythrocyte ghosts is not enhanced by hepes. ... Der Ausfluss der Nukleotide, in isotonischem Cholinchlorid, wurde von Hepes nicht beeinflusst. ...
Looking for SIGMA-ALDRICH Hepes,Contain 250g,CAS 7365-45-9,Glass (45ZE86)? Graingers got your back. Price:$240.50. Easy ...
If we add Hepes buffer into any bacterial culture, can it degrade the protein in the cells? ... HEPES BUFFER and protein degradation - posted in Protein and Proteomics: ... If we add Hepes buffer into any bacterial culture, can it degrade the protein in the cells? ... HEPES BUFFER and protein degradation. Started by Siniya, Apr 07 2018 03:51 AM ...
DMEM (Dulbeccos Modified Eagle Medium) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM include primary fibroblasts, neurons, glial cells, HUVECs, and smooth muscle cells, as well as cell lines such as HeLa, 293, Cos-7, a
Learn more about HEPES sodium salt. We enable science by offering product choice, services, process excellence and our people ...
Hanks with HEPES,Hanks with HEPES,Hanks with HEPES ... HBSS with 10 mM HEPES, Without Phenol Red. Hanks Balanced Salt ... Hanks Balanced Salt Solution (HBSS) modified with 10 mM HEPES, without phenol red ...
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Home/ Forums/ General: Student Questions/ URGENT: composition of Hepes Buffered saline. URGENT: composition of Hepes Buffered ... HEPES 10mM;. NaCl 151mM;. KCl 4.7mM;. CaCl2 2mM;. MgCl2 1.2mM;. glucose 7.8mM. ... Toxicity of light-exposed Hepes media. Lepe-Zuniga JL, Zigler JS Jr, Gery I. Journal of Immunological Methods. 1987 Oct 23;103( ... Analysis of the cytotoxic effects of light-exposed HEPES-containing culture medium.. * Zigler JS Jr, Lepe-Zuniga JL, Vistica B ...
HEPES Buffered Saline Solution. HEPES Buffered Saline Solution (30 mM) available in different sizes. ...
Recommended Storage : 15°C to 30°C (Do not freeze); Keep container tightly closed. Keep container in a cool, well-ventilated area. Do not store above 20°C (68°F).. ...
Synonyms: 4-(2-Hydroxyethyl)piperazin-1-ylethanesulfonic acid, HEPES free acid. One of the best general-purpose buffers for ...
This graph shows the total number of publications written about "HEPES" by people in this website by year, and whether "HEPES" ... "HEPES" is a descriptor in the National Library of Medicines controlled vocabulary thesaurus, MeSH (Medical Subject Headings). ... Below are the most recent publications written about "HEPES" by people in Profiles. ...
BioAssay record AID 357113 submitted by ChEMBL: Antibacterial activity against Escherichia coli K12 ATCC 23716 in 10% Luria-Bertani medium/90% Hepes buffer at pH 7.0.
MEM, originally prepared by Harry Eagle, is one of the most popular cell culture media. Upon his attempts to cultivate normal mammalian fibroblasts and certain HeLa cell subtypes, it was revealed that the nutritional needs of these cell types could not be met by BME. Further studies led to the development of MEM incorporating specific modifications such as higher amino acid concentrations for the cultivation of fastidious cells. MPs MEM may be used to support the growth of cells in monolayers, in suspension and wide variety of other cell types with proper supplementation. MP offers MEM with either Earles or Hanks salts.
HEPES, 25 mM in RPMI and DMEM is like water in that its dissociation decreases as the temperature decreases. This makes HEPES ... DFP17-50LT , DMEM / Hams F-12 w/ L-glutamine and 15 mM HEPES w/o phenol red and sodium bicarbonate size: 50 L , 87.50 USD ... DFP17-10LT , DMEM / Hams F-12 w/ L-glutamine and 15 mM HEPES w/o phenol red and sodium bicarbonate size: 10 L , 65.35 USD ... DFL25-500ML , DMEM / Hams F-12 w/ L-glutamine and 15mM HEPES w/o choline chloride and phenol red size: 500 mL , 73.10 USD ...
Learn details and find deals on HEPES, sodium salt from AG Scientific, the leading supplier of biochemical raw materials for ...
Learn more about HEPES Buffered Saline Solution 2X and get the best prices in the life science marketplace at AG Scientific, ...
... augmentation by a HEPES buffer. Rongbao Zhao, Mitra Najmi, Srinivas Aluri, David C Spray and I. David Goldman ... augmentation by a HEPES buffer. Rongbao Zhao, Mitra Najmi, Srinivas Aluri, David C Spray and I. David Goldman ... augmentation by a HEPES buffer. Rongbao Zhao, Mitra Najmi, Srinivas Aluri, David C Spray and I. David Goldman ...
Binding affinity to human antithrombin in HEPES buffer containing 0.25 M sodium chloride assessed as increase of serpin ...
HEPES Hemisodium. (4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid hemisodium salt; N-(2-Hydroxyethyl)piperazine-N′-(2- ... Click the button below to add the HEPES Hemisodium 25 g to your wish list. ...
HEPES is a zwitterionic organic chemical buffering agent. HEPES is commonly added to media at concentrations ranging from 10 mM ... Recently, HEPES has found an increasing role outside the area of biochemisty such as in the field of nanoparticles2,3,4,5. ... HEPES, Free Acid. (N-[2-Hydroxyethyl]piperazine-N-[2-Ethanesulfonic Acid]; 4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid) ... Depending on your application the use of HEPES with Cu(II) should be carefully considered as a possible interaction may occur1. ...
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the Pub Med ID of your paper to get a coupon. ...
  • HEPES (N-2-hydroxyethylpiperazine-N-2-ethane sulfonic acid) is a general purpose zwitterionic organic chemical buffering agent which does not bind magnesium, calcium, manganese(II) or copper (II) ions. (mpbio.com)
  • 1) Hegetschweiler and Saltman, Interaction of copper(II) with N-(2-hydroxyethyl)piperazine-N'-ethanesulfonic acid (HEPES), Inorg. (p212121.com)
  • In this work, three-dimensional branched gold nanocrystals were produced at high yield by reacting an aqueous solution of chloroauric acid with a Good's buffer, HEPES, at room temperature. (p212121.com)
  • Gibco® HEPES (N-2-hydroxyethylpiperazine-N-2-ethane sulfonic acid) is a zwitterionic organic chemical buffering agent commonly used in cell culture media. (ualberta.ca)
  • A HEPES that is ethanesulfonic acid in which one of the methyl hydrogens is replaced by a 4-(2-hydroxyethyl)piperazin-1-yl group. (ebi.ac.uk)
  • HEPES (N-(2-Hydroxyethyl)piperazine-N'-2-ethanesulfonic Acid)) is a zwitterionic organic chemical buffering agent. (reportshub.biz)
  • HEPES is widely used in cell culture, largely because it is better at maintaining physiological pH despite changes in carbon dioxide concentration (produced by aerobic respiration) when compared to bicarbonate buffers, which are also commonly used in cell culture. (wikipedia.org)
  • reported an unwanted photochemical process wherein HEPES when exposed to ambient light produces hydrogen peroxide, which is not a problem in bicarbonate-based cell culture buffers. (wikipedia.org)
  • HEPES is a zwitterionic biological buffer and is one of Good's buffers. (sigmaaldrich.com)
  • HEPES is better at maintaining physiological pH in cell culture when compared to bicarbonate buffers despite the changes in carbon dioxide concentration 1 . (sigmaaldrich.com)
  • Organic amine-based buffer compounds such as HEPES (Good's buffers) are commonly applied in experimental systems, including those where the biological effects of peroxynitrite are studied. (semanticscholar.org)
  • HEPES has been described as one of the best all-purpose buffers available for biological research. (ufcbio.com)
  • HEPES is one of the most effective buffers in the important physiological pH range of 7.2 to 7.6. (ufcbio.com)
  • We suggest that PIPES and HEPES buffers should be utilized with caution for fixation when examining the interplay between cells and their extracellular environment, especially in Drosophila pupal and adult retina research. (biomedcentral.com)
  • The K+-dependence of the reaction velocity in the presence of either TRIS/HCl or HEPES/KOH buffers at pH 7.5, is shown in Figure 3D. (ctskinhibito.com)
  • HEPES hemisodium is a zwitterionic organic chemical buffering agent. (p212121.com)
  • 1 Similar to many amino acids, HEPES is zwitterionic at most biological pHs. (ufcbio.com)
  • There are 15 Chapters to deeply display the global HEPES market. (reportshub.biz)
  • A new business intelligence report released by Market Research with title "Global HEPES market Research Report 2019" that targets and provides comprehensive market analysis with prospects to 2025. (amarketresearchgazette.com)
  • HEPES is widely used in cell culture, molecular biology and biochemical researches, largely because it is better at maintaining physiological pH.Compared to 2015, global HEPES market managed to increase revenue by 1.83 percent to 0.791 million USD in 2016. (amarketresearchgazette.com)
  • Merck KGaA is the biggest supplier in the global HEPES market. (amarketresearchgazette.com)
  • The report evaluates the global HEPES market size, share, and growth rate and also provides an accurate projection for similar facets by thoroughly studying historic as well as current status of the market. (amarketresearchgazette.com)
  • Studies have indicated that 20 mM HEPES is the most satisfactory concentration of the buffer when both Hanks' and Earle's solutions are used. (mpbio.com)
  • Maximum activation was reached at different K+ concentrations depending on the buffering species: in TRIS/HCl buffer, 100 mM K+ was the most effective, whereas in HEPES/KOH buffer, maximum activity was reached at about 200 mM K+ concentration. (ctskinhibito.com)
  • HEPES, 25 mM in RPMI and DMEM is like water in that its dissociation decreases as the temperature decreases. (gentaur.com)
  • This makes HEPES RPMI 25 milli-molar media with or without L-glutamine a more effective buffering agent for maintaining enzyme structure and function at low temperatures. (gentaur.com)
  • what is the exact composition of the Hepes Buffered saline reagent? (scientistsolutions.com)
  • HEPES Buffered Saline Solution (30 mM) available in different sizes. (promocell.com)
  • A buffer solution of HEPES can be prepared by any of several methods. (mpbio.com)
  • HEPES Buffer Solution is a biological buffer used in cell culture media. (stemcell.com)
  • Seedless, surfactantless, high-yield synthesis of branched gold nanocrystals in HEPES buffer solution, Chem. (p212121.com)
  • Thermo Scientific Precise™ Protein Gels are precast polyacrylamide gels for protein electrophoresis that use Tris-HEPES running buffer, have long shelf lives, and provide compatibility with different commercial gel tanks. (thermofisher.com)
  • In addition to Precise Tris-HEPES gels, we offer many other minigels in various formulations. (thermofisher.com)
  • Chart of resolution and migration profiles for Thermo Scientific Precise Protein Gels (Tris-HEPES Gels). (thermofisher.com)
  • replacement of HEPES/KOH buffer with TRIS/HCl abolished the enzymatic activity unless a monovalent cation was present (Figure 3C). (ctskinhibito.com)
  • doi:10.1371/journal.pone.0065595.gpresence of 100 mM K+, TRIS buffer was the best at sustaining activity, followed by MOPS, HEPES, and Phosphate. (ctskinhibito.com)
  • A simple mixing table for preparing 0.05 M HEPES/NaOH has been published. (mpbio.com)
  • HEPES is a good buffering choice for many cell culture systems because it is membrane impermeable, has limited effect on biochemical reactions, is chemically and enzymatically stable, and has very low visible and UV light absorbance. (mpbio.com)
  • and two literature-known HPLC assays for HEPES quantification were evaluated. (ejnmmigateway.net)
  • provided a reasonable quantification of HEPES using HPLC. (ejnmmigateway.net)
  • It is therefore strongly advised to keep HEPES-containing solutions in darkness as much as possible to prevent oxidation. (wikipedia.org)
  • So its strongly advised to keep any HEPES containing solution in dark. (scientistsolutions.com)
  • HEPES is the buffer of choice in a protein deposition technique in electron microscopy because it did not affect metal substrates. (mpbio.com)
  • If we add Hepes buffer into any bacterial culture, can it degrade the protein in the cells? (protocol-online.org)
  • HybridoXL Medium With L-Glutamine, HEPES buffer, Insulin and Sodium bicarbonate is supplied by HiMedia. (biomall.in)
  • HEPES is used in many media because it has more buffering capacity than sodium bicarbonate at physiological pH (7.2 - 7.4) at 37°C. Sodium bicarbonate is nutritionally necessary for most cells, so HEPES should be added in addition to, not in place of, sodium bicarbonate. (biosera.com)
  • HEPES is widely used in cell culture, molecular biology and biochemical researches, largely because it is better at maintaining physiological pH. (reportshub.biz)
  • HEPES is the recommended buffer for the glutamate binding assay because it prevents binding to non-receptor materials. (mpbio.com)
  • Importantly keep in mind phototoxicity of HEPES when exposed to ambient light due to the production of hydrogen peroxide. (scientistsolutions.com)
  • Alternatively, equimolar concentrations of HEPES and of HEPES sodium salt can be mixed in approximately equal volumes and back-titrate with either solution to the appropriate pH. (mpbio.com)
  • HEPES - A buffering salt. (astm.org)
  • HEPES is commonly added to media at concentrations ranging from 10 mM to 25 mM. (p212121.com)
  • HEPES buffer commonly used in cell culture media. (mpbio.com)
  • Store at Room Temperature (15-30°C). HEPES is light sensitive when added to media. (mpbio.com)
  • Hydrogen peroxide formation by reaction of peroxynitrite with HEPES and related tertiary amines. (semanticscholar.org)
  • HEPES does not bind magnesium, calcium or manganese(II) ions 2 . (sigmaaldrich.com)
  • The addition of 10-25 mM HEPES provides extra buffering capacity when cell culture requires extended periods of manipulation outside of a CO 2 incubator. (mpbio.com)
  • HEPES has been utilized extensively for research and commercial applications in the cell culture and tissue culture industries. (ufcbio.com)
  • And the Cell Culture application is the biggest application, the HEPES is mainly used in the Cell Culture application. (amarketresearchgazette.com)
  • Most solutions of HEPES hemisodium will dissolve in water forming a pH at 7.5 with little to no adjustment. (mpbio.com)
  • HEPES has been evaluated and shown to be suitable for use with Ampholines in generating pH gradients less than 1 pH unit wide for isolectric focusing applications. (mpbio.com)