Buffers
Tromethamine
An organic amine proton acceptor. It is used in the synthesis of surface-active agents and pharmaceuticals; as an emulsifying agent for cosmetic creams and lotions, mineral oil and paraffin wax emulsions, as a biological buffer, and used as an alkalizer. (From Merck, 11th ed; Martindale, The Extra Pharmacopoeia, 30th ed, p1424)
Bicarbonates
Hydrogen-Ion Concentration
4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid
Lichens
Physician Assistants
Health professionals who practice medicine as members of a team with their supervising physicians. They deliver a broad range of medical and surgical services to diverse populations in rural and urban settings. Duties may include physical exams, diagnosis and treatment of disease, interpretation of tests, assist in surgery, and prescribe medications. (from http://www.aapa.orglabout-pas accessed 2114/2011)
Salts
Sodium
Phenolsulfonphthalein
Phenolphthaleins
Culture Media
Any liquid or solid preparation made specifically for the growth, storage, or transport of microorganisms or other types of cells. The variety of media that exist allow for the culturing of specific microorganisms and cell types, such as differential media, selective media, test media, and defined media. Solid media consist of liquid media that have been solidified with an agent such as AGAR or GELATIN.
Cricetinae
Carbonic Anhydrase I
DNA Damage
Injuries to DNA that introduce deviations from its normal, intact structure and which may, if left unrepaired, result in a MUTATION or a block of DNA REPLICATION. These deviations may be caused by physical or chemical agents and occur by natural or unnatural, introduced circumstances. They include the introduction of illegitimate bases during replication or by deamination or other modification of bases; the loss of a base from the DNA backbone leaving an abasic site; single-strand breaks; double strand breaks; and intrastrand (PYRIMIDINE DIMERS) or interstrand crosslinking. Damage can often be repaired (DNA REPAIR). If the damage is extensive, it can induce APOPTOSIS.
Catalogs as Topic
Prostaglandins F, Synthetic
Physical Fitness
Sodium Bicarbonate
Cells, Cultured
Fibroblasts
Organic Chemicals
Nanoparticles
Multiple Myeloma
A malignancy of mature PLASMA CELLS engaging in monoclonal immunoglobulin production. It is characterized by hyperglobulinemia, excess Bence-Jones proteins (free monoclonal IMMUNOGLOBULIN LIGHT CHAINS) in the urine, skeletal destruction, bone pain, and fractures. Other features include ANEMIA; HYPERCALCEMIA; and RENAL INSUFFICIENCY.
Publications
Glutamine
Click Chemistry
Organic chemistry methodology that mimics the modular nature of various biosynthetic processes. It uses highly reliable and selective reactions designed to "click" i.e., rapidly join small modular units together in high yield, without offensive byproducts. In combination with COMBINATORIAL CHEMISTRY TECHNIQUES, it is used for the synthesis of new compounds and combinatorial libraries.
Bibliometrics
Transcriptional regulation of the esp genes of enterohemorrhagic Escherichia coli. (1/141)
We have determined that the genes encoding the secreted proteins EspA, EspD, and EspB of enterohemorrhagic Escherichia coli (EHEC) are organized in a single operon. The esp operon is controlled by a promoter located 94 bp upstream from the ATG start codon of the espA gene. The promoter is activated in the early logarithmic growth phase, upon bacterial contact with eukaryotic cells and in response to Ca2+, Mn2+, and HEPES. Transcription of the esp operon seems to be switched off in tightly attached bacteria. The activation process is regulated by osmolarity (induction at high osmolarities), modulated by temperature, and influenced by the degree of DNA supercoiling. Transcription is sigmaS dependent, and the H-NS protein contributes to its fine tuning. Identification of the factors involved in activation of the esp operon and the signals responsible for modulation may facilitate understanding of the underlying molecular events leading to sequential expression of virulence factors during natural infections caused by EHEC. (+info)Generation of intracellular pH gradients in single cardiac myocytes with a microperfusion system. (2/141)
This study describes the use of a microperfusion system to create rapid, large regional changes in intracellular pH (pH(i)) within single ventricular myocytes. The spatial distribution of pH(i) in single myocytes was measured with seminaphthorhodafluor-1 fluorescence using confocal imaging. Changes in pH(i) were induced by local external application of NH(4)Cl, CO(2), or sodium propionate. Local application was achieved by simultaneously directing two parallel square microstreams, each 275 microm wide, over a single myocyte oriented perpendicular to the direction of flow. One stream contained the control solution, and the other contained a weak acid or base. End-to-end, stable pH(i) gradients as large as 1 pH unit were readily created with this technique. This result indicates that pH within a single cardiac cell may not always be spatially uniform, particularly when weak acid or base gradients are present, which can occur, for example, in regional myocardial ischemia. The microperfusion method should be useful for studying the effects of localized acidosis on myocyte function, estimating intracellular ion diffusion rates, and, possibly, inducing regional changes in other important intracellular ions. (+info)Augmentation of L-type calcium current by hypoxia in rabbit carotid body glomus cells: evidence for a PKC-sensitive pathway. (3/141)
Previous studies have suggested that voltage-gated Ca(2+) influx in glomus cells plays a critical role in sensory transduction at the carotid body chemoreceptors. The purpose of the present study was to determine the effects of hypoxia on the Ca(2+) current in glomus cells and to elucidate the underlying mechanism(s). Experiments were performed on freshly dissociated glomus cells from rabbit carotid bodies. Ca(2+) current was monitored using the whole cell configuration of the patch-clamp technique, with Ba(2+) as the charge carrier. Hypoxia (pO(2) = 40 mmHg) augmented the Ca(2+) current by 24 +/- 3% (n = 42, at 0 mV) in a voltage-independent manner. This effect was seen in a CO(2)/HCO(3)(-)-, but not in a HEPES-buffered extracellular solution at pH 7.4 (n = 6). When the pH of a HEPES-buffered extracellular solution was lowered from 7.4 to 7. 0, hypoxia augmented the Ca(2+) current by 20 +/- 5% (n = 4, at 0 mV). Nisoldipine, an L-type Ca(2+) channel blocker (2 microM, n = 6), prevented, whereas, omega-conotoxin MVIIC (2 microM, n = 6), an inhibitor of N and P/Q type Ca(2+) channels, did not prevent augmentation of the Ca(2+) current by hypoxia, implying that low oxygen affects L-type Ca(2+) channels in glomus cells. Protein kinase C (PKC) inhibitors, staurosporine (100 nM, n = 6) and bisindolylmaleimide (2 microM, n = 8, at 0 mV), prevented, whereas, a protein kinase A inhibitor (4 nM PKAi, n = 10) did not prevent the hypoxia-induced increase of the Ca(2+) current. Phorbol 12-myristate 13-acetate (PMA, 100 nM), a PKC activator, augmented the Ca(2+) current by 20 +/- 3% (n = 8, at 0 mV). In glomus cells treated with PMA overnight (100 nM), hypoxia did not augment the Ca(2+) current (-3 + 4%, n = 5, at 0 mV). Immunocytochemical analysis revealed PKCdelta-like immunoreactivity in the cytosol of the glomus cells. Following hypoxia (6% O(2) for 5 min), PKCdelta-like immunoreactivity translocated to the plasma membrane in 87 +/- 3% of the cells, indicating PKC activation. These results demonstrate that hypoxia augments Ca(2+) current through L-type Ca(2+) channels via a PKC-sensitive mechanism. (+info)Pore block versus intrinsic gating in the mechanism of inward rectification in strongly rectifying IRK1 channels. (4/141)
The IRK1 channel is inhibited by intracellular cations such as Mg(2+) and polyamines in a voltage-dependent manner, which renders its I-V curve strongly inwardly rectifying. However, even in excised patches exhaustively perfused with a commonly used artificial intracellular solution nominally free of Mg(2+) and polyamines, the macroscopic I-V curve of the channels displays modest rectification. This observation forms the basis of a hypothesis, alternative to the pore-blocking hypothesis, that inward rectification reflects the enhancement of intrinsic channel gating by intracellular cations. We find, however, that residual rectification is caused primarily by the commonly used pH buffer HEPES and/or some accompanying impurity. Therefore, inward rectification in the strong rectifier IRK1, as in the weak rectifier ROMK1, can be accounted for by voltage-dependent block of its ion conduction pore by intracellular cations. (+info)Contributions of protein disulfide isomerase domains to its chaperone activity. (5/141)
Protein disulfide isomerase (PDI), a member of the thioredoxin (Trx) superfamily, consists of five consecutive domains, a-b-b'-a'-c. Domain combinations, AB, A'C, B'A'C and AB-C, and hybrids of PDI domains with Trx, Trx-C and Trx-B'A'C, have been constructed and expressed in Escherichia coli to examine the contributions of PDI domains to its enzyme and chaperone activities. All the combination and hybrid products are considerably less active than intact PDI in their enzyme activities. Recombinant products containing C, at low concentrations, inhibit the reactivation of lysozyme in HEPES buffer, while those without C do not. Only the intact PDI molecule and the hybrid molecule, Trx-B'A'C, but to a much lower level, show general chaperone activity in assisting the reactivation of denatured D-glyceraldehyde-3-phosphate dehydrogenase. It is suggested that all domains of PDI contribute to the binding of target protein for its chaperone activity. (+info)Mechanisms of endothelial cell swelling from lactacidosis studied in vitro. (6/141)
One of the early sequelae of ischemia is an increase of circulating lactic acid that occurs in response to anaerobic metabolism. The purpose of the present study was to investigate whether lactic acidosis can induce endothelial swelling in vitro under closely controlled extracellular conditions. Cell volume of suspended cultured bovine aortic endothelial cells was measured by use of an advanced Coulter technique employing the "pulse area analysis" signal-processing technique (CASY1). The isosmotic reduction of pH from 7.4 to 6.8 had no effect on cell volume. Lowering of pH to 6.6, 6.4, or 6.0, however, led to significant, pH-dependent increases of cell volume. Swelling was more pronounced in bicarbonate-buffered media than in HEPES buffer. Specific inhibition of Na(+)/H(+) exchange by ethylisopropylamiloride completely prevented swelling in HEPES-buffered media. Pretreatment with ouabain to partially depolarize the cells did not affect the degree of acidosis-induced swelling. In bicarbonate-buffered media, the inhibition of transmembrane HCO(3)(-) transport by DIDS reduced swelling to a level comparable with that seen in the absence of bicarbonate ions. Lactacidosis-induced endothelial swelling, therefore, is a result of intracellular pH regulatory mechanisms, namely, Na(+)/H(+) exchange and bicarbonate-transporting carriers. (+info)Regulation of the epithelial Na(+) channel by extracellular acidification. (7/141)
The effect of extracellular acidification was tested on the native epithelial Na(+) channel (ENaC) in A6 epithelia and on the cloned ENaC expressed in Xenopus oocytes. Channel activity was determined utilizing blocker-induced fluctuation analysis in A6 epithelia and dual electrode voltage clamp in oocytes. In A6 cells, a decrease of extracellular pH (pH(o)) from 7.4 to 6.4 caused a slow stimulation of the amiloride-sensitive short-circuit current (I(Na)) by 68.4 +/- 11% (n = 9) at 60 min. This increase of I(Na) was attributed to an increase of open channel and total channel (N(T)) densities. Similar changes were observed with pH(o) 5.4. The effects of pH(o) were blocked by buffering intracellular Ca(2+) with 5 microM 1, 2-bis(2-aminophenoxy)ethane-N,N,N',N'-tetraacetic acid. In oocytes, pH(o) 6.4 elicited a small transient increase of the slope conductance of the cloned ENaC (11.4 +/- 2.2% at 2 min) followed by a decrease to 83.7 +/- 11.7% of control at 60 min (n = 6). Thus small decreases of pH(o) stimulate the native ENaC by increasing N(T) but do not appreciably affect ENaC expressed in Xenopus oocytes. These effects are distinct from those observed with decreasing intracellular pH with permeant buffers that are known to inhibit ENaC. (+info)Binding of dystrophin's tandem calponin homology domain to F-actin is modulated by actin's structure. (8/141)
Dystrophin has been shown to be associated in cells with actin bundles. Dys-246, an N-terminal recombinant protein encoding the first 246 residues of dystrophin, includes two calponin-homology (CH) domains, and is similar to a large class of F-actin cross-linking proteins including alpha-actinin, fimbrin, and spectrin. It has been shown that expression or microinjection of amino-terminal fragments of dystrophin or the closely related utrophin resulted in the localization of these protein domains to actin bundles. However, in vitro studies have failed to detect any bundling of actin by either intact dystrophin or Dys-246. We show here that the structure of F-actin can be modulated so that there are two modes of Dys-246 binding, from bundling actin filaments to only binding to single filaments. The changes in F-actin structure that allow Dys-246 to bundle filaments are induced by covalent modification of Cys-374, proteolytic cleavage of F-actin's C-terminus, mutation of yeast actin's N-terminus, and different buffers. The present results suggest that F-actin's structural state can have a large influence on the nature of actin's interaction with other proteins, and these different states need to be considered when conducting in vitro assays. (+info)
HEPES, Free Acid | CAS 7365-45-9 | Goods Buffer | P212121 Store
Establishment of a standardized post-embedding method for immunoelectron microscopy by applying heat-induced antigen retrieval
Buy HybridoXL Medium w/ L-Glutamine, HEPES buffer, Insulin and 1.5g/L NaHCO3 AL206A-500ML in India | Biomall
Buy DMEM/ F12, 1:1 mixture w/ HEPES Buffer w/o L-Glutamine, Trace elements and NaHCO3 AT140A-20L in India | Biomall
HEPES (Fine White Crystals/Molecular Biology), Fisher BioReagents Poly Bottle; 500g HEPES (Fine White Crystals/Molecular...
Gentaur Molecular :Biosera \ MEM w Earles Salts w L Glutamine w o Sodium Bicarbonate w 25mM Hepes For 10L \...
Gentaur Molecular :Biosera \ DMEM F12 w L Glutamine w o Sodium Bicarbonate w 15 mM Hepes For 5L \ PM-D1227/5
Insulin solution from bovine pancreas 10 mg/mL insulin in 25 mM HEPES, pH 8.2, BioReagent, sterile-filtered, suitable for cell...
Trapp Stimulates Guanine Nucleotide Exchange on Ypt1p | Journal of Cell Biology | Rockefeller University Press
Neuromics: April 2015
Plus it
May | 2020 | Plk signal
Plus it
Generation of intracellular pH gradients in single cardiac myocytes with a microperfusion system. - Oxford Neuroscience
An East Asian Common Variant Vinculin P.Asp841His Was Associated With Sudden Unexplained Nocturnal Death Syndrome in the...
International Journal of Pharmaceutics (v.378, #1-2) | www.chemweb.com
He catalytic activity; replacement of HEPES/KOH buffer with TRIS/HCl | CTSK Inhibito ctskinhibito.com
Iscoves Modification of DMEM - Iscoves Modification of DMEM - Classical Media - Products
Minimum Essential Medium Eagle (Modified) (1X Solution) With Hanks Salts, 20 mM HEPES, Without L-Glutamine, Sodium Bicarbonate
Ecdysone-induced expression of the caspase DRONC during hormone-dependent programmed cell death in Drosophila is regulated by...
Plus it
NVS-PAK1-1 | Chemical Probes
P0192-N1L - IMDM w/ L-Glutamine w/ 25 mM Hepes w/o Phenol Red - Jain Biologicals
Mangelplasmen - Haemochrom
pHi Regulation in Myocardium of the Spontaneously Hypertensive Rat | Circulation Research
0.006404</PDBx:fract transf...
Plus it
Iscoves Modified Dulbeccos Medium (IMDM) - Caisson Laboratories, Inc.
A Turn-Off Red-emitting fluorophore for nanomolar detection of Heparin
Human Retina-RPE-Choroidea Perfusion Tissue Culture: An in vitro Model to Investigate Retinal Pigmentepitheliopathies in Man |...
Plus it
Plus it
DMEM, high glucose, HEPES, no phenol red
bmse000792 HEPES - Supplemental Proton Data at BMRB
HEPES, Free Acid, High Purity | BioExpress
HEPES Sodium Salt Specification Comparison | China-Mainland | Sigma-Aldrich
SuperCult® RPMI 1640 without HEPES and L-glutamine | Creative Bioarray
Entecavir | HIV Infection & Hepes Treatment | Online Pharmacy
Hampton Research
Tubastatin A | ≥99%(HPLC) | Selleck | HDAC inhibitor
...
PENN SCAP-T Protocols | SCAP-T
PENN SCAP-T Protocols | SCAP-T
JCI Insight -
LipidFinder: A computational workflow for discovery of lipids identifies eicosanoid-phosphoinositides in platelets
Dulbeccos Modified Eagles/Hams F-12 Medium 1:1 (DMEF) - Caisson Laboratories, Inc.
Prostate cells accumulate great cellular and mitochondrial concentrations of zinc, generally | CDK-II inhibitor KN-62 increase...
Roles of Amino Acids and Subunits in Determining the Inhibition of Nicotinic Acetylcholine Receptors by Competitive Antagonists...
Ethanesulfonic acid 594-45-6, China Ethanesulfonic acid 594-45-6 Manufacturers, China Ethanesulfonic acid 594-45-6 Suppliers
Mechanism of TRPM7 Channel Inhibition by 2-Aminoethyl Diphenyl Borinat by Rikki Chokshi and J. Ashot Kozak
Widespread Inhibition of Sodium Channel-dependent Glutamate Release from Isolated Nerve Terminals by Isoflurane and Propofol |...
Cation effects on cell shape.<...
Pleiotropic effects of apolipoprotein A-Ⅱ on high-density lipoprotein functionality, adipose tissue metabolic activity and...
JCI -
Regulation of expression of the multidrug resistance protein MRP1 by p53 in human prostate cancer cells
USE OF 5-H-DIBENZ/B,F/AZEPINE-5-CARBOXAMIDE DERIVATIVES IN THE TREATMENT OF NEUROPATHIC PAIN AND NEUROLOGICAL DISORDERS -...
COLLAGEN AND FIBRIN MICROTHREADS IN A DISCRETE THREAD MODEL OF IN VITRO ACL SCAFFOLD REGENERATION - Patent application
Chen medium
Summary Report | CureHunter
Hams F12 (1X Solution) With L-Glutamine
Kinetic characterization of the interaction of the Z-fragment of protein A with mouse-IgG3 in a volume in chemical space.
CRYOcheck™ Factor V Deficient Plasma
SureFire Optimized Bolt Carrier-Long Stroke (OBC-LS) Drop-In BCG and H7S Buffer System with Longer Action Spring for Better...
A Dihydropyridine-sensitive Voltage-dependent Calcium Channel in the Sarcolemmal Membrane of Crustacean Muscle | JGP
Conditional, Genetically Encoded, Small Molecule-Regulated Inhibition of NFκB Signaling in RPE Cells | IOVS | ARVO Journals
Modulation of Tight Junction Proteins in the Perineurium to Facilitate Peripheral Opioid Analgesia | Anesthesiology | American...
Indian Patents. 214369:A DIBENZOAZULENE COMPOUND
Plus it
JCI -
Immune synapses between mast cells and γδ T cells limit viral infection
2-{[(3α,5β,6α,7α,17ξ)-3,6,7-Trihydroxy-24-oxocholan-24-yl]amino}ethanesulfonic acid | C26H45NO7S | ChemSpider
2-{[(3α,5β,12α,17α,20S)-3,12-Dihydroxy-24-oxocholan-24-yl]amino}ethanesulfonic acid | C26H45NO6S | ChemSpider
ERK1 Antibody (Monoclonal, 33A5)
how long can it take for herpes simplex 2 to show up. how can i prevent future beeakouts? | Answers from Doctors | HealthTap
What is a buffer? PreLab 3.5
org mode - Is there a possibility to have multiple agenda views open at once? - Emacs Stack Exchange
Reactive oxygen species production in marine microalgae
Stimulation by Hepes buffer". The Biochemical Journal. 254 (2): 519-23. doi:10.1042/bj2540519. PMC 1135108. PMID 3178771. K., ...
Lysis buffer
HEPES - NaOH 7.2 - 8.2 Additives[edit]. Salts[edit]. Lysis buffer usually contains one or more salts. The function of salts in ...
HEPPS (buffer)
CAPSO CHES HEPES Good, N. E; Winget, G. D; Winter, W; Connolly, T. N; Izawa, S; Singh, R. M (1966). "Hydrogen ion buffers for ...
Yepes
... when it was called Hepes, which in turn would produce Hiepes, Iepes and finally Yepes. It is possible that Hepes be a mozarabic ...
Carbamoyl phosphate synthetase
The activity of the enzyme is known to be inhibited by both Tris and HEPES buffers. Carbamoyl phosphate synthase (CPSase) is a ... by Tris and Hepes. Effect on Ka for N-acetylglutamate". Biochem. J. 243 (1): 273-276. doi:10.1042/bj2430273. PMC 1147843. PMID ...
Wikipedia:WikiProject Chemicals/Log/2010-01-28
13:33:09 (3, 2, 4) (EDIT) User:71.245.207.73 (contribs, talk) edited HEPES (diff, hist). Added links: http://www.labome.com/ ... review/HEPES.html. *. 13:36:57 (2, 4, 4) (EDIT) User:Beetstra (contribs, talk) edited Cysteine (diff, hist). Added: 'InChIKey ...
MOPS
HEPES is a similar pH buffering compound that contains a piperazine ring. With a pKa of 7.20, MOPS is an excellent buffer for ...
Diethyl pyrocarbonate
For this reason, DEPC cannot be used with Tris or HEPES buffers. In contrast, it can be used with phosphate-buffered saline or ...
Saline (medicine)
HEPES-buffered saline (HBS) (recipes from Dittmar, Liu, Ausubel etc.). *Gey's balanced salt solution (GBSS) ...
Good's buffers
HEPES, POPSO and EPPS) can form radicals and should be avoided in studies of redox processes in biochemistry. Tricine is photo- ... "Hydrogen peroxide formation by reaction of peroxynitrite with HEPES and related tertiary amines. Implications for a general ...
ACP1
1dg9: CRYSTAL STRUCTURE OF BOVINE LOW MOLECULAR WEIGHT PTPASE COMPLEXED WITH HEPES ...
Embryo culture
Seems to have detrimental effects in embryo development in vitro; HEPES-buffered medium: used as buffered medium for human ... oocyte collection and embryo handling; MOPS-buffered medium: like HEPES, has the potential advantage that the buffering ...
PIPES
MOPS HEPES MES Tris Common buffer compounds used in biology Good's buffers Good, Norman E.; Winget, G. Douglas; Winter, ...
Cell isolation
The acidity of the solution must be regulated, often using a pH buffer such as HEPES. Isolation of cells from some tissues may ...
Antibiotic-Antimycotic
Negrete, Alejandro; Ling, Tau; Lyddiatt, Andrew (2008). "Effect of pluronic F-68, 5% CO2 atmosphere, HEPES, and Antibiotic- ...
Buffer solution
HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid). 7.48. −0.014. 238.3 TES (2-[[1,3-dihydroxy-2-(hydroxymethyl)propan- ...
Tricine
Hicks, M., and Gebicki, J. M., "Rate constants for reaction of hydroxyl radicals with Tris, Tricine, and Hepes buffers." "FEBS ...
Tris
MOPS HEPES MES Common buffer compounds used in biology Gomori, G., Preparation of Buffers for Use in Enzyme Studies. Methods ...
Transfection
HEPES-buffered saline solution (HeBS) containing phosphate ions is combined with a calcium chloride solution containing the DNA ...
NUN buffer
... contains: HEPES [pH 7.6], Urea, NaCl, DDT, PIC 1 & 2, 1.1% NP-40, Sodium orthovanadate, β-glycerol phosphate and ...
HEPBS
... and HEPES have very similar structures and properties, HEPBS also having an acidity (pKa) in the physiological range (7.6 ...
MES (buffer)
Retrieved 2012-09-10.CS1 maint: archived copy as title (link) CAPS (buffer) CHES MOPS HEPES HEPPS Tris Common buffer compounds ...
Particle-induced X-ray emission
In order to get a meaningful sulfur signal from the analysis, the buffer should not contain sulfur (i.e. no BES, DDT, HEPES, ...
Hyperpolarized carbon-13 MRI
For example, in a study detecting tumor response to etoposide treatment, the sample was dissolved in 40 mM HEPES, 94 mM NaOH, ...
Minusheet perfusion culture system
For example, application of 50 mmol/l HEPES or an equivalent of BUFFER ALL (ca. 1%) to IMDM (Iscove's Modified Dulbecco's ... 50 mmol/l HEPES) equilibrated against atmospheric air during a standard perfusion culture experiment shows constant partial ... a CO2 incubator has to be stabilized by reducing the NaHCO3 concentration and/or by adding biological buffers such as HEPES ( ...
List of MeSH codes (D03)
... hepes MeSH D03.383.606.515 - hydroxyzine MeSH D03.383.606.515.200 - cetirizine MeSH D03.383.606.527 - 1-(5-isoquinolinesulfonyl ...
HBS
HEPES-buffered Saline (HBS) Hexagonal bilayer silica Hoboken Shore Railroad, a defunct railway in New Jersey, US Honmon ...
Crystallization adjutant
Buffer molecules can become part of the lattice (for example HEPES in becomes incorporated in crystals of human neutrophil ...
Fixation (histology)
Hepes-glutamic acid buffer-mediated organic solvent protection effect (HOPE) gives formalin-like morphology, excellent ...
HEPES - Wikipedia
HEPES has the following characteristics: pKa1 (25 °C) = 3 pKa2 (25 °C) = 7.5 Useful pH range = 2.5 to 3.5 or 6.8 to 8.2 HEPES ... HEPES is widely used in cell culture, largely because it is better at maintaining physiological pH despite changes in carbon ... HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid) is a zwitterionic sulfonic acid buffering agent; one of the twenty ... Zigler JS, Lepe-Zuniga JL, Vistica B, Gery I (May 1985). "Analysis of the cytotoxic effects of light-exposed HEPES-containing ...
HEPES-buffer
HEPES Specification Comparison | China-Mainland | Sigma-Aldrich
HEPES does not bind magnesium, calcium or manganese(II) ions2.. HEPES is not recommended for certain protein applications; it ... HEPES is a zwitterionic biological buffer and is one of Goods buffers. HEPES is better at maintaining physiological pH in cell ... HEPES sodium salt. 75277-39-3. C8H17N2NaO4S. 260.29. H7006 H3784 5380-OP. H8651. H3784. RES6007H-A7 H3662 ... HEPES (free acid). 7365-45-9. C8H18N2O4S. 238.30. RDD002. H3375. 5310-OP. 391338. 54457. H7523. H4034. H6147. PHG0001 RES6003H- ...
1M HEPES Buffer
Studies have indicated that 20 mM HEPES is the most satisfactory concentration of the buffer when both Hanks and Earles ... HEPES (N-2-hydroxyethylpiperazine-N-2-ethane sulfonic acid) is a general purpose zwitterionic organic chemical buffering agent ... HEPES: 238300.00 mg/liter , Solution Product Families Description HEPES (N-2-hydroxyethylpiperazine-N-2-ethane sulfonic acid) ... HEPES buffer commonly used in cell culture media. The addition of 10-25 mM HEPES provides extra buffering capacity when cell ...
HEPES Sodium Salt Specification Comparison | China-Mainland | Sigma-Aldrich
Precise™ Tris-HEPES Gels | Thermo Fisher Scientific - CA
Chart of resolution and migration profiles for Thermo Scientific Precise Protein Gels (Tris-HEPES Gels). Values in each lane ... Precise Tris-HEPES gel features *High-performance-unique running buffer helps produces excellent separation and high-resolution ... Precise Tris-HEPES gel selection table. Gel concentration. 10-well. 12-well. 15-well. ... In addition to Precise Tris-HEPES gels, we offer many other minigels in various formulations. Find the right one for your ...
HEPES Buffer Solution (1M) | STEMCELL Technologies
HEPES Buffer Solution is a biological buffer for cell culture media. Selection of suitable nutrient medium depends on cell type ... Internal Search Keywords: HEPES buffer,HEPES solution,HIPS solution,HIPIS solution,HEEPS,7200 ... HEPES Buffer Solution is a biological buffer used in cell culture media. Selection of suitable nutrient medium is dependent on ...
Alfa Aesar HEPES Lysis Buffer with NP-40 250mL:Life Sciences
Efflux of cyclic AMP from resealed erythrocyte ghosts is not enhanced by hepes | SpringerLink
Der Ausfluss der Nukleotide, in isotonischem Cholinchlorid, wurde von Hepes nicht beeinflusst. ... Hepes Ghost Erythrocyte Ghost Reseal Erythrocyte Ghost Cholinchlorid This work was supported by the Cystic Fibrosis Research ... Efflux of cyclic AMP from resealed erythrocyte ghosts is not enhanced by hepes. ... Der Ausfluss der Nukleotide, in isotonischem Cholinchlorid, wurde von Hepes nicht beeinflusst. ...
SIGMA-ALDRICH Hepes,Contain 250g,CAS 7365-45-9,Glass - 45ZE86|H3375-250G - Grainger
HEPES BUFFER and protein degradation - Protein and Proteomics - BioForum
If we add Hepes buffer into any bacterial culture, can it degrade the protein in the cells? ... HEPES BUFFER and protein degradation - posted in Protein and Proteomics: ... If we add Hepes buffer into any bacterial culture, can it degrade the protein in the cells? ... HEPES BUFFER and protein degradation. Started by Siniya, Apr 07 2018 03:51 AM ...
DMEM, high glucose, HEPES, no phenol red
HEPES sodium salt | BioExpress
HBSS with 10 mM HEPES, Without Phenol Red | STEMCELL Technologies
7365-45-9 - HEPES, 0.5M buffer soln., pH 7.0 - J60064 - Alfa Aesar
URGENT: composition of Hepes Buffered saline | Scientist Solutions
Home/ Forums/ General: Student Questions/ URGENT: composition of Hepes Buffered saline. URGENT: composition of Hepes Buffered ... HEPES 10mM;. NaCl 151mM;. KCl 4.7mM;. CaCl2 2mM;. MgCl2 1.2mM;. glucose 7.8mM. ... Toxicity of light-exposed Hepes media. Lepe-Zuniga JL, Zigler JS Jr, Gery I. Journal of Immunological Methods. 1987 Oct 23;103( ... Analysis of the cytotoxic effects of light-exposed HEPES-containing culture medium.. * Zigler JS Jr, Lepe-Zuniga JL, Vistica B ...
HEPES Buffered Saline Solution | PromoCell
HEPES, Free Acid, ULTROL™ Grade, 1.0 M Solution, Calbiochem™, EMD Millipore
HEPES, Free Acid, High Purity | BioExpress
HEPES | Profiles RNS
This graph shows the total number of publications written about "HEPES" by people in this website by year, and whether "HEPES" ... "HEPES" is a descriptor in the National Library of Medicines controlled vocabulary thesaurus, MeSH (Medical Subject Headings). ... Below are the most recent publications written about "HEPES" by people in Profiles. ...
AID 357113 - Antibacterial activity against Escherichia coli K12 ATCC 23716 in 10% Luria-Bertani medium/90% Hepes buffer at pH...
Minimum Essential Medium Eagle (Modified) (1X Solution) With Hank's Salts, 20 mM HEPES, Without L-Glutamine, Sodium Bicarbonate
MEM, originally prepared by Harry Eagle, is one of the most popular cell culture media. Upon his attempts to cultivate normal mammalian fibroblasts and certain HeLa cell subtypes, it was revealed that the nutritional needs of these cell types could not be met by BME. Further studies led to the development of MEM incorporating specific modifications such as higher amino acid concentrations for the cultivation of fastidious cells. MPs MEM may be used to support the growth of cells in monolayers, in suspension and wide variety of other cell types with proper supplementation. MP offers MEM with either Earles or Hanks salts.
DMEM / Ham's F-12 w/ L-glutamine and 15mM HEPES w/o choline
HEPES, 25 mM in RPMI and DMEM is like water in that its dissociation decreases as the temperature decreases. This makes HEPES ... DFP17-50LT , DMEM / Hams F-12 w/ L-glutamine and 15 mM HEPES w/o phenol red and sodium bicarbonate size: 50 L , 87.50 USD ... DFP17-10LT , DMEM / Hams F-12 w/ L-glutamine and 15 mM HEPES w/o phenol red and sodium bicarbonate size: 10 L , 65.35 USD ... DFL25-500ML , DMEM / Hams F-12 w/ L-glutamine and 15mM HEPES w/o choline chloride and phenol red size: 500 mL , 73.10 USD ...
Buy HEPES, sodium salt | CAS 75277-39-3 | Cell Culture | AG Scientific
HEPES Buffered Saline Solution 2X | CAS 7365-45-9 | Buffer | AG Sci.
Concentrative transport of antifolates mediated by the proton-coupled folate transporter (PCFT, SLC46A1); augmentation by a...
... augmentation by a HEPES buffer. Rongbao Zhao, Mitra Najmi, Srinivas Aluri, David C Spray and I. David Goldman ... augmentation by a HEPES buffer. Rongbao Zhao, Mitra Najmi, Srinivas Aluri, David C Spray and I. David Goldman ... augmentation by a HEPES buffer. Rongbao Zhao, Mitra Najmi, Srinivas Aluri, David C Spray and I. David Goldman ...
AID 536873 - Binding affinity to human antithrombin in HEPES buffer containing 0.25 M sodium chloride assessed as increase of...
HEPES Hemisodium | CAS 103404-87-1 | Buffer | P212121 Store
HEPES, Free Acid | CAS 7365-45-9 | Good's Buffer | P212121 Store
HEPES is a zwitterionic organic chemical buffering agent. HEPES is commonly added to media at concentrations ranging from 10 mM ... Recently, HEPES has found an increasing role outside the area of biochemisty such as in the field of nanoparticles2,3,4,5. ... HEPES, Free Acid. (N-[2-Hydroxyethyl]piperazine-N-[2-Ethanesulfonic Acid]; 4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid) ... Depending on your application the use of HEPES with Cu(II) should be carefully considered as a possible interaction may occur1. ...
SuperCult® RPMI 1640 without HEPES and L-glutamine | Creative Bioarray
Acid6
- HEPES (N-2-hydroxyethylpiperazine-N-2-ethane sulfonic acid) is a general purpose zwitterionic organic chemical buffering agent which does not bind magnesium, calcium, manganese(II) or copper (II) ions. (mpbio.com)
- 1) Hegetschweiler and Saltman, Interaction of copper(II) with N-(2-hydroxyethyl)piperazine-N'-ethanesulfonic acid (HEPES), Inorg. (p212121.com)
- In this work, three-dimensional branched gold nanocrystals were produced at high yield by reacting an aqueous solution of chloroauric acid with a Good's buffer, HEPES, at room temperature. (p212121.com)
- Gibco® HEPES (N-2-hydroxyethylpiperazine-N-2-ethane sulfonic acid) is a zwitterionic organic chemical buffering agent commonly used in cell culture media. (ualberta.ca)
- A HEPES that is ethanesulfonic acid in which one of the methyl hydrogens is replaced by a 4-(2-hydroxyethyl)piperazin-1-yl group. (ebi.ac.uk)
- HEPES (N-(2-Hydroxyethyl)piperazine-N'-2-ethanesulfonic Acid)) is a zwitterionic organic chemical buffering agent. (reportshub.biz)
Buffers9
- HEPES is widely used in cell culture, largely because it is better at maintaining physiological pH despite changes in carbon dioxide concentration (produced by aerobic respiration) when compared to bicarbonate buffers, which are also commonly used in cell culture. (wikipedia.org)
- reported an unwanted photochemical process wherein HEPES when exposed to ambient light produces hydrogen peroxide, which is not a problem in bicarbonate-based cell culture buffers. (wikipedia.org)
- HEPES is a zwitterionic biological buffer and is one of Good's buffers. (sigmaaldrich.com)
- HEPES is better at maintaining physiological pH in cell culture when compared to bicarbonate buffers despite the changes in carbon dioxide concentration 1 . (sigmaaldrich.com)
- Organic amine-based buffer compounds such as HEPES (Good's buffers) are commonly applied in experimental systems, including those where the biological effects of peroxynitrite are studied. (semanticscholar.org)
- HEPES has been described as one of the best all-purpose buffers available for biological research. (ufcbio.com)
- HEPES is one of the most effective buffers in the important physiological pH range of 7.2 to 7.6. (ufcbio.com)
- We suggest that PIPES and HEPES buffers should be utilized with caution for fixation when examining the interplay between cells and their extracellular environment, especially in Drosophila pupal and adult retina research. (biomedcentral.com)
- The K+-dependence of the reaction velocity in the presence of either TRIS/HCl or HEPES/KOH buffers at pH 7.5, is shown in Figure 3D. (ctskinhibito.com)
Zwitterionic2
- HEPES hemisodium is a zwitterionic organic chemical buffering agent. (p212121.com)
- 1 Similar to many amino acids, HEPES is zwitterionic at most biological pHs. (ufcbio.com)
Global HEPES market5
- There are 15 Chapters to deeply display the global HEPES market. (reportshub.biz)
- A new business intelligence report released by Market Research with title "Global HEPES market Research Report 2019" that targets and provides comprehensive market analysis with prospects to 2025. (amarketresearchgazette.com)
- HEPES is widely used in cell culture, molecular biology and biochemical researches, largely because it is better at maintaining physiological pH.Compared to 2015, global HEPES market managed to increase revenue by 1.83 percent to 0.791 million USD in 2016. (amarketresearchgazette.com)
- Merck KGaA is the biggest supplier in the global HEPES market. (amarketresearchgazette.com)
- The report evaluates the global HEPES market size, share, and growth rate and also provides an accurate projection for similar facets by thoroughly studying historic as well as current status of the market. (amarketresearchgazette.com)
Concentration2
- Studies have indicated that 20 mM HEPES is the most satisfactory concentration of the buffer when both Hanks' and Earle's solutions are used. (mpbio.com)
- Maximum activation was reached at different K+ concentrations depending on the buffering species: in TRIS/HCl buffer, 100 mM K+ was the most effective, whereas in HEPES/KOH buffer, maximum activity was reached at about 200 mM K+ concentration. (ctskinhibito.com)
238.31
- Disolve HEPES(238.3 g) in 800 ml H 2 O. (edu-wiki.org)
RPMI2
- HEPES, 25 mM in RPMI and DMEM is like water in that its dissociation decreases as the temperature decreases. (gentaur.com)
- This makes HEPES RPMI 25 milli-molar media with or without L-glutamine a more effective buffering agent for maintaining enzyme structure and function at low temperatures. (gentaur.com)
Saline2
- what is the exact composition of the Hepes Buffered saline reagent? (scientistsolutions.com)
- HEPES Buffered Saline Solution (30 mM) available in different sizes. (promocell.com)
Buffer solution3
- A buffer solution of HEPES can be prepared by any of several methods. (mpbio.com)
- HEPES Buffer Solution is a biological buffer used in cell culture media. (stemcell.com)
- Seedless, surfactantless, high-yield synthesis of branched gold nanocrystals in HEPES buffer solution, Chem. (p212121.com)
Tris5
- Thermo Scientific Precise™ Protein Gels are precast polyacrylamide gels for protein electrophoresis that use Tris-HEPES running buffer, have long shelf lives, and provide compatibility with different commercial gel tanks. (thermofisher.com)
- In addition to Precise Tris-HEPES gels, we offer many other minigels in various formulations. (thermofisher.com)
- Chart of resolution and migration profiles for Thermo Scientific Precise Protein Gels (Tris-HEPES Gels). (thermofisher.com)
- replacement of HEPES/KOH buffer with TRIS/HCl abolished the enzymatic activity unless a monovalent cation was present (Figure 3C). (ctskinhibito.com)
- doi:10.1371/journal.pone.0065595.gpresence of 100 mM K+, TRIS buffer was the best at sustaining activity, followed by MOPS, HEPES, and Phosphate. (ctskinhibito.com)
NaOH1
- A simple mixing table for preparing 0.05 M HEPES/NaOH has been published. (mpbio.com)
Biological1
- HEPES, Biological Buffer is as it's name suggests, a buffering agent. (spectrumchemical.com)
Absorbance1
- HEPES is a good buffering choice for many cell culture systems because it is membrane impermeable, has limited effect on biochemical reactions, is chemically and enzymatically stable, and has very low visible and UV light absorbance. (mpbio.com)
Quantification2
- and two literature-known HPLC assays for HEPES quantification were evaluated. (ejnmmigateway.net)
- provided a reasonable quantification of HEPES using HPLC. (ejnmmigateway.net)
Strongly advised to keep2
- It is therefore strongly advised to keep HEPES-containing solutions in darkness as much as possible to prevent oxidation. (wikipedia.org)
- So its strongly advised to keep any HEPES containing solution in dark. (scientistsolutions.com)
Protein2
- HEPES is the buffer of choice in a protein deposition technique in electron microscopy because it did not affect metal substrates. (mpbio.com)
- If we add Hepes buffer into any bacterial culture, can it degrade the protein in the cells? (protocol-online.org)
Glutamine1
- HybridoXL Medium With L-Glutamine, HEPES buffer, Insulin and Sodium bicarbonate is supplied by HiMedia. (biomall.in)
Physiological pH2
- HEPES is used in many media because it has more buffering capacity than sodium bicarbonate at physiological pH (7.2 - 7.4) at 37°C. Sodium bicarbonate is nutritionally necessary for most cells, so HEPES should be added in addition to, not in place of, sodium bicarbonate. (biosera.com)
- HEPES is widely used in cell culture, molecular biology and biochemical researches, largely because it is better at maintaining physiological pH. (reportshub.biz)
Assay1
- HEPES is the recommended buffer for the glutamate binding assay because it prevents binding to non-receptor materials. (mpbio.com)
Exposed to ambient light1
- Importantly keep in mind phototoxicity of HEPES when exposed to ambient light due to the production of hydrogen peroxide. (scientistsolutions.com)
Salt2
Concentrations1
- HEPES is commonly added to media at concentrations ranging from 10 mM to 25 mM. (p212121.com)
Commonly1
- HEPES buffer commonly used in cell culture media. (mpbio.com)
Room Temperature1
- Store at Room Temperature (15-30°C). HEPES is light sensitive when added to media. (mpbio.com)
Hydrogen1
- Hydrogen peroxide formation by reaction of peroxynitrite with HEPES and related tertiary amines. (semanticscholar.org)
Magnesium1
- HEPES does not bind magnesium, calcium or manganese(II) ions 2 . (sigmaaldrich.com)
Cell3
- The addition of 10-25 mM HEPES provides extra buffering capacity when cell culture requires extended periods of manipulation outside of a CO 2 incubator. (mpbio.com)
- HEPES has been utilized extensively for research and commercial applications in the cell culture and tissue culture industries. (ufcbio.com)
- And the Cell Culture application is the biggest application, the HEPES is mainly used in the Cell Culture application. (amarketresearchgazette.com)
Solutions1
- Most solutions of HEPES hemisodium will dissolve in water forming a pH at 7.5 with little to no adjustment. (mpbio.com)
Suitable1
- HEPES has been evaluated and shown to be suitable for use with Ampholines in generating pH gradients less than 1 pH unit wide for isolectric focusing applications. (mpbio.com)