The destruction of ERYTHROCYTES by many different causal agents such as antibodies, bacteria, chemicals, temperature, and changes in tonicity.
A condition of inadequate circulating red blood cells (ANEMIA) or insufficient HEMOGLOBIN due to premature destruction of red blood cells (ERYTHROCYTES).
Red blood cells. Mature erythrocytes are non-nucleated, biconcave disks containing HEMOGLOBIN whose function is to transport OXYGEN.
The presence of free HEMOGLOBIN in the URINE, indicating hemolysis of ERYTHROCYTES within the vascular system. After saturating the hemoglobin-binding proteins (HAPTOGLOBINS), free hemoglobin begins to appear in the urine.
RED BLOOD CELL sensitivity to change in OSMOTIC PRESSURE. When exposed to a hypotonic concentration of sodium in a solution, red cells take in more water, swell until the capacity of the cell membrane is exceeded, and burst.
A syndrome of HEMOLYSIS, elevated liver ENZYMES, and low blood platelets count (THROMBOCYTOPENIA). HELLP syndrome is observed in pregnant women with PRE-ECLAMPSIA or ECLAMPSIA who also exhibit LIVER damage and abnormalities in BLOOD COAGULATION.
A disease-producing enzyme deficiency subject to many variants, some of which cause a deficiency of GLUCOSE-6-PHOSPHATE DEHYDROGENASE activity in erythrocytes, leading to hemolytic anemia.
The semi-permeable outer structure of a red blood cell. It is known as a red cell 'ghost' after HEMOLYSIS.
Acquired hemolytic anemia due to the presence of AUTOANTIBODIES which agglutinate or lyse the patient's own RED BLOOD CELLS.
Proteins from BACTERIA and FUNGI that are soluble enough to be secreted to target ERYTHROCYTES and insert into the membrane to form beta-barrel pores. Biosynthesis may be regulated by HEMOLYSIN FACTORS.
A condition characterized by the recurrence of HEMOGLOBINURIA caused by intravascular HEMOLYSIS. In cases occurring upon cold exposure (paroxysmal cold hemoglobinuria), usually after infections, there is a circulating antibody which is also a cold hemolysin. In cases occurring during or after sleep (paroxysmal nocturnal hemoglobinuria), the clonal hematopoietic stem cells exhibit a global deficiency of cell membrane proteins.
The oxygen-carrying proteins of ERYTHROCYTES. They are found in all vertebrates and some invertebrates. The number of globin subunits in the hemoglobin quaternary structure differs between species. Structures range from monomeric to a variety of multimeric arrangements.
Plasma glycoproteins that form a stable complex with hemoglobin to aid the recycling of heme iron. They are encoded in man by a gene on the short arm of chromosome 16.
A test to detect non-agglutinating ANTIBODIES against ERYTHROCYTES by use of anti-antibodies (the Coombs' reagent.) The direct test is applied to freshly drawn blood to detect antibody bound to circulating red cells. The indirect test is applied to serum to detect the presence of antibodies that can bind to red blood cells.
Diazo derivatives of aniline, used as a reagent for sugars, ketones, and aldehydes. (Dorland, 28th ed)
A disease characterized by chronic hemolytic anemia, episodic painful crises, and pathologic involvement of many organs. It is the clinical expression of homozygosity for hemoglobin S.
A method to identify and enumerate cells that are synthesizing ANTIBODIES against ANTIGENS or HAPTENS conjugated to sheep RED BLOOD CELLS. The sheep red blood cells surrounding cells secreting antibody are lysed by added COMPLEMENT producing a clear zone of HEMOLYSIS. (From Illustrated Dictionary of Immunology, 3rd ed)
Agglutination of ERYTHROCYTES by a virus.
An antigenic mismatch between donor and recipient blood. Antibodies present in the recipient's serum may be directed against antigens in the donor product. Such a mismatch may result in a transfusion reaction in which, for example, donor blood is hemolyzed. (From Saunders Dictionary & Encyclopedia of Laboratory Medicine and Technology, 1984).
Hemolytic anemia due to the ingestion of fava beans or after inhalation of pollen from the Vicia fava plant by persons with glucose-6-phosphate dehydrogenase deficient erythrocytes.
Any of the ruminant mammals with curved horns in the genus Ovis, family Bovidae. They possess lachrymal grooves and interdigital glands, which are absent in GOATS.
Serum glycoproteins participating in the host defense mechanism of COMPLEMENT ACTIVATION that creates the COMPLEMENT MEMBRANE ATTACK COMPLEX. Included are glycoproteins in the various pathways of complement activation (CLASSICAL COMPLEMENT PATHWAY; ALTERNATIVE COMPLEMENT PATHWAY; and LECTIN COMPLEMENT PATHWAY).
The number of RETICULOCYTES per unit volume of BLOOD. The values are expressed as a percentage of the ERYTHROCYTE COUNT or in the form of an index ("corrected reticulocyte index"), which attempts to account for the number of circulating erythrocytes.
Abnormal intracellular inclusions, composed of denatured hemoglobin, found on the membrane of red blood cells. They are seen in thalassemias, enzymopathies, hemoglobinopathies, and after splenectomy.
A bile pigment that is a degradation product of HEME.
The aggregation of ERYTHROCYTES by AGGLUTININS, including antibodies, lectins, and viral proteins (HEMAGGLUTINATION, VIRAL).
A 150-kDa serum glycoprotein composed of three subunits with each encoded by a different gene (C8A; C8B; and C8G). This heterotrimer contains a disulfide-linked C8alpha-C8gamma heterodimer and a noncovalently associated C8beta chain. C8 is the next component to bind the C5-7 complex forming C5b-8 that binds COMPLEMENT C9 and acts as a catalyst in the polymerization of C9.
Immunizing agent containing IMMUNOGLOBULIN G anti-Rho(D) used for preventing Rh immunization in Rh-negative individuals exposed to Rh-positive red blood cells.
The taking of a blood sample to determine its character as a whole, to identify levels of its component cells, chemicals, gases, or other constituents, to perform pathological examination, etc.
Yellow discoloration of the SKIN; MUCOUS MEMBRANE; and SCLERA in the NEWBORN. It is a sign of NEONATAL HYPERBILIRUBINEMIA. Most cases are transient self-limiting (PHYSIOLOGICAL NEONATAL JAUNDICE) occurring in the first week of life, but some can be a sign of pathological disorders, particularly LIVER DISEASES.
The senescence of RED BLOOD CELLS. Lacking the organelles that make protein synthesis possible, the mature erythrocyte is incapable of self-repair, reproduction, and carrying out certain functions performed by other cells. This limits the average life span of an erythrocyte to 120 days.
A nutritional condition produced by a deficiency of VITAMIN E in the diet, characterized by posterior column and spinocerebellar tract abnormalities, areflexia, ophthalmoplegia, and disturbances of gait, proprioception, and vibration. In premature infants vitamin E deficiency is associated with hemolytic anemia, thrombocytosis, edema, intraventricular hemorrhage, and increasing risk of retrolental fibroplasia and bronchopulmonary dysplasia. An apparent inborn error of vitamin E metabolism, named familial isolated vitamin E deficiency, has recently been identified. (Cecil Textbook of Medicine, 19th ed, p1181)
A group of familial congenital hemolytic anemias characterized by numerous abnormally shaped erythrocytes which are generally spheroidal. The erythrocytes have increased osmotic fragility and are abnormally permeable to sodium ions.
Exotoxins produced by certain strains of streptococci, particularly those of group A (STREPTOCOCCUS PYOGENES), that cause HEMOLYSIS.
A tetrameric enzyme that, along with the coenzyme NAD+, catalyzes the interconversion of LACTATE and PYRUVATE. In vertebrates, genes for three different subunits (LDH-A, LDH-B and LDH-C) exist.
The number of RED BLOOD CELLS per unit volume in a sample of venous BLOOD.
Oxygen-carrying RED BLOOD CELLS in mammalian blood that are abnormal in structure or function.
The effects, both local and systemic, caused by the bites of SPIDERS.
The major human blood type system which depends on the presence or absence of two antigens A and B. Type O occurs when neither A nor B is present and AB when both are present. A and B are genetic factors that determine the presence of enzymes for the synthesis of certain glycoproteins mainly in the red cell membrane.
A condition characterized by an abnormal increase of BILIRUBIN in the blood, which may result in JAUNDICE. Bilirubin, a breakdown product of HEME, is normally excreted in the BILE or further catabolized before excretion in the urine.
C5 plays a central role in both the classical and the alternative pathway of COMPLEMENT ACTIVATION. C5 is cleaved by C5 CONVERTASE into COMPLEMENT C5A and COMPLEMENT C5B. The smaller fragment C5a is an ANAPHYLATOXIN and mediator of inflammatory process. The major fragment C5b binds to the membrane initiating the spontaneous assembly of the late complement components, C5-C9, into the MEMBRANE ATTACK COMPLEX.
Small glycoproteins found on both hematopoietic and non-hematopoietic cells. CD59 restricts the cytolytic activity of homologous complement by binding to C8 and C9 and blocking the assembly of the membrane attack complex. (From Barclay et al., The Leukocyte Antigen FactsBook, 1993, p234)
Ability of ERYTHROCYTES to change shape as they pass through narrow spaces, such as the microvasculature.
Serologic tests in which a known quantity of antigen is added to the serum prior to the addition of a red cell suspension. Reaction result is expressed as the smallest amount of antigen which causes complete inhibition of hemagglutination.
A species of gram-negative, aerobic bacteria that is the etiologic agent of epidemic typhus fever acquired through contact with lice (TYPHUS, EPIDEMIC LOUSE-BORNE) as well as Brill's disease.
Stable chromium atoms that have the same atomic number as the element chromium, but differ in atomic weight. Cr-50, 53, and 54 are stable chromium isotopes.
A 105-kDa serum glycoprotein with significant homology to the other late complement components, C7-C9. It is a polypeptide chain cross-linked by 32 disulfide bonds. C6 is the next complement component to bind to the membrane-bound COMPLEMENT C5B in the assembly of MEMBRANE ATTACK COMPLEX. It is encoded by gene C6.
The process by which blood or its components are kept viable outside of the organism from which they are derived (i.e., kept from decay by means of a chemical agent, cooling, or a fluid substitute that mimics the natural state within the organism).
A spider of the genus Loxosceles, found in the midwestern and other parts of the United States, which carries a hemolytic venom that produces local necrosis or ulceration.
The species Oryctolagus cuniculus, in the family Leporidae, order LAGOMORPHA. Rabbits are born in burrows, furless, and with eyes and ears closed. In contrast with HARES, rabbits have 22 chromosome pairs.
Plasmids controlling the synthesis of hemolysin by bacteria.
An examination of chemicals in the blood.
An autosomal recessive glycogen storage disease in which there is deficient expression of 6-phosphofructose 1-kinase in muscle (PHOSPHOFRUCTOKINASE-1, MUSCLE TYPE) resulting in abnormal deposition of glycogen in muscle tissue. These patients have severe congenital muscular dystrophy and are exercise intolerant.
A 93-kDa serum glycoprotein encoded by C7 gene. It is a polypeptide chain with 28 disulfide bridges. In the formation of MEMBRANE ATTACK COMPLEX; C7 is the next component to bind the C5b-6 complex forming a trimolecular complex C5b-7 which is lipophilic, resembles an integral membrane protein, and serves as an anchor for the late complement components, C8 and C9.
The type (and only) species of RUBIVIRUS causing acute infection in humans, primarily children and young adults. Humans are the only natural host. A live, attenuated vaccine is available for prophylaxis.
Solutions that have a lesser osmotic pressure than a reference solution such as blood, plasma, or interstitial fluid.
The most common etiologic agent of GAS GANGRENE. It is differentiable into several distinct types based on the distribution of twelve different toxins.

Changes in haematological parameters and iron metabolism associated with a 1600 kilometre ultramarathon. (1/3524)

OBJECTIVE: To investigate haematological variations and iron related changes in the serum of participants in a 1600 kilometre ultramarathon run. PARTICIPANTS: Seven male and two female participants in a 1600 km foot race. METHODS: Blood samples were obtained from the participants before, after four and 11 days of running, and at the end of the event. Samples were analysed by standard methods for haemoglobin, packed cell volume, total red cell count, mean red cell volume, mean red cell haemoglobin, total white cell count and differential, platelets, reticulocytes, iron, ferritin, total iron binding capacity, percentage transferrin saturation, haptoglobin, and bilirubin and corrected for changes in plasma volume. RESULTS: The following variables decreased during the event (p < 0.05): haemoglobin, packed cell volume, mean red cell volume, percentage lymphocytes, percentage monocytes, serum iron, total iron binding capacity, and percentage transferrin saturation. Increases (p < 0.05) were found in plasma volume, total red cell count (day 4 only), total white cell count, percentage and absolute numbers of neutrophils and reticulocytes, absolute numbers of lymphocytes and monocytes (day 4 only), absolute numbers of eosinophils (day 11 and race end), absolute numbers of basophils (race end only), platelets, ferritin, haptoglobin, and bilirubin (day 4 only). CONCLUSION: Ultramarathon running is associated with a wide range of changes in haematological parameters, many of which are related to the normal acute phase response to injury. These should not be confused with indicators of disease.  (+info)

Improved methods using the reverse transcriptase polymerase chain reaction to detect tumour cells. (2/3524)

Reverse transcriptase polymerase chain reaction (RT-PCR) is increasingly used to detect small numbers of circulating tumour cells, though the clinical benefit remains controversial. The largest single contributing factor to the controversy of its value is the different approaches to sample processing. The aim of this study was to compare the sensitivity and reproducibility of RT-PCR for the detection of tumour cells after four commonly used different methods of sample processing. Using RT-PCR, one tumour cell spiked in 2 ml of whole blood was detected after analysis of separated mononuclear cell RNA, whole blood total or poly-A+ RNA. No false positives were identified with any method. However, the reproducibility of tumour cell detection was reduced after isolation of the mononuclear cell fraction. Only analysis of poly-A+ RNA had a sensitivity of 100% in all the cell spiking experiments. In patient blood samples, analysis of poly-A+ RNA increased the number of blood samples positive for tyrosine hydroxylase (TH) mRNA compared with those positive after analysis of total RNA. This may reflect high levels of cDNA reducing the efficiency of the PCR. Isolation of poly-A+ RNA increases the sensitivity and reproducibility of tumour cell detection in peripheral blood.  (+info)

Antioxidative activity of 4-oxy- and 4-hydroxy-nitroxides in tissues and erythrocytes from rats. (3/3524)

AIM: To compare the activities of antioxidation of 4-oxy- and 4-hydroxy-nitroxides in tissues and RBC from rats. METHODS: The homogenates of liver, heart, and kidneys of rats were used to determine malondialdehyde (MDA) formation using TBA colorimetric method. H2O2-caused hemolysis was measured spectrometrically. Superoxide anion from zymosan-stimulated neutrophils of rats was assayed by NBT reduction method. RESULTS: Nitroxide free radicals OTMPO and HTMPO inhibited MDA generation caused by .OH generation system (MIC 10.5 and 21 mumol.L-1, respectively), antagonized hemolysis induced by H2O2 (MIC: 338 and 168 mumol.L-1, respectively), but did not affect O2- formation from activated neutrophils. 1-Hydroxyl compounds OTMPOH and HTMPOH possessed similarly potent antilipoperoxidative activities. But nonfree radical OTMP and HTMP had no effect on peroxidation of tissues. CONCLUSION: Nitroxides exert their antilipoperoxidative effect by specifically scavenging .OH free radicals in biological system. Trapping of .OH free radicals by nitroxides is not by reduction of NO. group in nitroxides. Both NO. group and NOH group are essential active groups.  (+info)

Isolation and characterization of Vibrio parahaemolyticus causing infection in Iberian toothcarp Aphanius iberus. (4/3524)

High mortality among laboratory cultured Iberian toothcarp Aphanius iberus occurred in February 1997 in Valencia (Spain). The main signs of the disease were external haemorrhage and tail rot. Bacteria isolated from internal organs of infected fish were biochemically homogeneous and identified as Vibrio parahaemolyticus. The bacteria were haemolytic against erythrocytes from eel Anguilla anguilla, amberjack Seriola dumerili, toothcarp A. iberus and humans, and were Kanagawa-phenomenon-negative. Infectivity tests showed that the virulence for A. iberus was dependent on salinity. Finally, all strains were virulent for amberjack and eel.  (+info)

Hemolysis associated with 25% human albumin diluted with sterile water--United States, 1994-1998. (5/3524)

Since 1994, a shortage of 5% human albumin, a product used off-label during therapeutic plasma exchange (TPE), has existed in the United States. Because of this shortage, hospital pharmacists may prepare 5% solution of human albumin by diluting 25% human albumin with 0.9% NaCl or, when sodium load is a concern, 5% dextrose. However, if sterile water alone is used as the diluent, the osmolarity (tonicity) of the albumin solution is reduced and may cause hemolysis in recipients. This report describes two of 10 episodes of hemolysis (one fatal) among persons who received 25% human albumin diluted with sterile water and emphasizes that sterile water alone should not be used to dilute albumin.  (+info)

Novel techniques for in vivo hemolysis studies in guinea pigs. (6/3524)

The in vivo toxic-hemolytic studies using small experimental animals are complicated by difficulties in preventing hemolysis during repeated collection of blood specimens and in measuring hemoglobin concentration in small amounts of plasma sample. To solve these problems we tried to develope the new techniques for the in vivo hemolysis studies using guinea pigs. The hemolysis accident was minimized to 2.75 mg/dl by collecting the blood directly into heparinized microhematocrit tubes by small longitudinal incision in the auricular artery. The hemoglobin in a small amount of sample (10 microliters) was determined by the new analytical system using a microflow spectrophotometer with a modified cyanmethemoglobin method. The standard curve of the hemoglobin concentration in the system revealed a line of Y = 1.8X + 0.79 (r = 0.999), CV < 1% with a minimum detectable concentration of 1.25 mg/dl. By using the new techniques, it was found that the plasma hemoglobin concentration in normal animals were 7.27 +/- 0.44 mg/dl (mean +/- S.E.). The in vivo hemolytic activity of saponin was observed dose-dependently at doses of 30-50 mg/kg, i.v. in the guinea pigs. It is concluded that the present techniques are useful for in vivo hemolytic studies in small experimental animals such as guinea pigs.  (+info)

The hemolytic activity of bracken extracts in guinea pigs. (7/3524)

This study was conducted to elucidate the hemolytic activity of a new toxic substance in bracken fern. A crude extract (CE) was prepared from the methanol extracts of bracken by the column chromatography. When the CE was injected subcutaneously in guinea pigs, the hemoglobinuria and hemolysis were observed within 6 hr, and 3 days later edema and hemorrhages in the urinary bladder were observed. The CE was then fractionated by high performance liquid chromatography (HPLC), and three (HF, BF and CF) of the fractions showed the toxic activities in guinea pigs. The HF caused the hemolysis, whereas both the BF and the CF caused the hemorrhagic cystitis without any hemolytic activities. The HF was further fractionated by the HPLC, resulting of the 3 fractions (HF-I, II and III). The hemolysis was caused only with the HF-II, and HF-II as well as HF did not cause the hemorrhagic cystitis. HPLC analysis revealed that both BF and CF contains braxin B and braxin C, respectively, and both HF and HF-II do not contain braxin A, B or C. These facts suggest that bracken fern contains a new toxic substance (hemolysin) which induces the acute hemolysis in guinea pigs.  (+info)

The rgg gene of Streptococcus pyogenes NZ131 positively influences extracellular SPE B production. (8/3524)

Streptococcus pyogenes produces several extracellular proteins, including streptococcal erythrogenic toxin B (SPE B), also known as streptococcal pyrogenic exotoxin B and streptococcal proteinase. Several reports suggest that SPE B contributes to the virulence associated with S. pyogenes; however, little is known about its regulation. Nucleotide sequence data revealed the presence, upstream of the speB gene, of a gene, designated rgg, that was predicted to encode a polypeptide similar to previously described positive regulatory factors. The putative Rgg polypeptide of S. pyogenes NZ131 consisted of 280 amino acids and had a predicted molecular weight of 33,246. To assess the potential role of Rgg in the production of SPE B, the rgg gene was insertionally inactivated in S. pyogenes NZ131, which resulted in markedly decreased SPE B production, as determined both by immunoblotting and caseinolytic activity on agar plates. However, the production of other extracellular products, including streptolysin O, streptokinase, and DNase, was not affected. Complementation of the rgg mutant with an intact rgg gene copy in S. pyogenes NZ131 could restore SPE B production and confirmed that the rgg gene product is involved in the production of SPE B.  (+info)

Simpson, M.A.; Gozzo, J.J., 1978: Spectrophotometric determination of lymphocyte mediated sheep red blood cell hemolysis in vitro
Non-physiologic turbulent flow occurs in medical cardiovascular devices resulting in hemodynamic stresses that may damage red blood cells (RBC) and cause hemolysis. Hemolysis was previously thought to result from Reynolds shear stress (RSS) in turbulent flows. A more recent hypothesis suggests that turbulent viscous shear stresses (TVSS) at spatial scales similar in size to RBCs are related to their damage. We applied two-dimensional digital particle image velocimetry to measure the flow field of a free-submerged axisymmetric jet that was utilized to hemolyze porcine RBCs in selected locations. Assuming a dynamic equilibrium for the sub-grid scale (SGS) energy flux between the resolved and the sub-grid scales, the SGS energy flux was calculated from the strain rate tensor computed from the resolved velocity fields. The SGS stress was determined by the Smagorinsky model, from which the turbulence dissipation rate and then TVSS were estimated. Our results showed the hemolytic threshold of the ...
Significance and Use 5.1 The presence of hemolytic material in contact with the blood may cause loss of, or damage to, red blood cells and may produce increased levels of free plasma hemoglobin capable of inducing toxic effects or other effects which may stress the kidneys or other organs. 5.2 This practice may not be predictive of events occurring during all types of implant applications. The user is cautioned to consider the appropriateness of the method in view of the materials being tested, their potential applications, and the recommendations contained in Practice F748. 1. Scope 1.1 This practice provides a protocol for the assessment of hemolytic properties of materials used in the fabrication of medical devices that will contact blood. 1.2 This practice is intended to evaluate the acute in vitro hemolytic properties of materials intended for use in contact with blood. 1.3 This practice consists of a
Blood Agar and Hemolysis Certain bacterial species produce extracellular enzymes that lyse red blood cells in the blood agar (hemolysis). These hemolysin (extotoxin) radially diffuses outwards from the colony (or colonies) causing complete or partial destruction of the red cells (RBC) in the medium and complete denaturation of hemoglobin within the cells to colorless products.. Four types of hemolysis are produced in Sheep blood agar by Streptococci namely; Alpha hemolysis, Beta hemolysis, gamma hemolysis and alpha prime or wide zone alpha hemolysis.. Hemolysis is best observed by examining colonies grown under anaerobic conditions or inspecting sub-surface colonies.. How does one know if the colonies they are observing on a plate have caused alpha hemolysis or beta hemolysis?. Note: To know the type of blood agar hemolysis, the blood agar plate must be held up to a light source and observed with the light coming from behind (transmitted light).. If either type of hemolysis is present, then one ...
Blood Agar and Hemolysis Certain bacterial species produce extracellular enzymes that lyse red blood cells in the blood agar (hemolysis). These hemolysin (extotoxin) radially diffuses outwards from the colony (or colonies) causing complete or partial destruction of the red cells (RBC) in the medium and complete denaturation of hemoglobin within the cells to colorless products.. Four types of hemolysis are produced in Sheep blood agar by Streptococci namely; Alpha hemolysis, Beta hemolysis, gamma hemolysis and alpha prime or wide zone alpha hemolysis.. Hemolysis is best observed by examining colonies grown under anaerobic conditions or inspecting sub-surface colonies.. How does one know if the colonies they are observing on a plate have caused alpha hemolysis or beta hemolysis?. Note: To know the type of blood agar hemolysis, the blood agar plate must be held up to a light source and observed with the light coming from behind (transmitted light).. If either type of hemolysis is present, then one ...
In vitro hemolysis can be an important unwanted effect in medical tests and can cause inaccurate results, because the contents of hemolysed red blood cells are included with the serum. The concentration of potassium inside red blood cells is much higher than in the serum and so an elevated potassium is usually found in biochemistry tests of hemolysed blood. If as little as 0.5% of the red blood cells are lysed the serum will have a visually obvious pinkish colour, due to hemoglobin. In vitro hemolysis can occur in a blood sample owing prolonged storage or storage in incorrect conditions (ie too hot, too cold). Hemolysis can also occur at the time of venipuncture, but it is uncommon when the venipuncture is straightforward and the phlebotomist is experienced. Excessive suction can cause the red blood cells to be literally smashed on their way through the hypodermic needle owing to turbulence and physical forces. Such hemolysis is more likely to occur when a patients veins are difficult to find ...
TY - JOUR. T1 - The effect of lead on oxidative hemolysis and erythrocyte defense mechanisms in the rat. AU - Gelman, B. B.. AU - Michaelson, I. A.. PY - 1977/1/1. Y1 - 1977/1/1. UR - UR - M3 - Article. AN - SCOPUS:0017414210. VL - 19. SP - No. 042. JO - Pharmacologist. JF - Pharmacologist. SN - 0031-7004. IS - 2. ER - ...
Intravascular erythrocyte destruction, accompanied by the release of pro-oxidative and pro-inflammatory components hemoglobin and heme, is a common event in the pathogenesis of numerous diseases with heterogeneous etiology and clinical features. A frequent adverse effect related to massive hemolysis is the renal injury and inflammation. Nevertheless, it is still unclear whether heme - a danger-associated molecular pattern and ligand for TLR4 or upstream hemolysis-derived products are responsible for these effects. Well-characterized animal models of hemolysis with kidney impairment are needed to investigate how hemolysis drives kidney injury and to test novel therapeutic strategies. Here we characterized the pathological processes leading to acute kidney injury and inflammation during massive intravascular hemolysis, using a mouse model of phenylhydrazine (PHZ)-triggered erythrocyte destruction. We observed profound changes in mRNA levels for markers of tubular damage (Kim-1, NGAL) and regeneration
Acute haemolysis associated with clinical episodes of high-level Plasmodium falciparum parasitaemia was studied in 20 children from an holoendemic area (coastal Tanzania). The change in blood haemoglobin (Hb) concentration ranged from -46 to +5 g/L during the 72-h observation period and was linearly related to maximum parasitaemia. Balance studies between loss of blood Hb, increase in plasma Hb and appearance of Hb in the urine indicated that extravascular clearance of red cells was the predominant mode of erythrocyte clearance. Most subjects, however, showed minor signs of intravascular haemolysis. The plasma Hb was ≪1% of blood Hb and haemoglobinuria was detected in 14/20 children but the excretion of Hb in urine was |0.5% of total Hb loss. Haemoglobinuria was, however, a marker of severe haemolysis, since the maximum blood Hb loss in children without haemoglobinuria was 10 g/L. Erythrocyte-bound opsonins known to induce erythrophagocytosis, i.e., complement C3c fragments and autologous IgG, were
Synonyms for alpha (alpha) hemolysis in Free Thesaurus. Antonyms for alpha (alpha) hemolysis. 3 synonyms for hemolysis: haematolysis, haemolysis, hematolysis. What are synonyms for alpha (alpha) hemolysis?
Calgary Laboratory Services provides information on the effects of hemolysis on clinical specimens because it can have an effect on laboratory results.
Hemolysis or haemolysis, also known by several other names, is the rupturing (lysis) of red blood cells (erythrocytes) and the release of their contents (cytoplasm) into surrounding fluid (e.g. blood plasma). Hemolysis may occur in vivo or in vitro (inside or outside the body). Hemolysins damage the host cytoplasmic membrane, causing cell lysis and death. The activity of these toxins is most easily observed with assays involving the lysis of red blood cells (erythrocytes). Some hemolysins attack the phospholipid of the host cytoplasmic membrane. Because the phospholipid lecithin (phosphatidylcholine) is often used as a substrate, these enzymes are called lecithinases or phospholipases. Some hemolysins affects the sterols of the host cytoplasmic membrane. Hemolysis inside the body can be caused by a large number of medical conditions, including many Gram-positive bacteria (e.g., Streptococcus, Enterococcus, and Staphylococcus), some parasites (e.g., Plasmodium), some autoimmune disorders (e.g., ...
Aurigon is a research institute dedicated to preclinical services for human and veterinary pharmaceuticals, food and chemicals. It provides a full range of advisory and experimental services in pharmacology, bio-/analytics and toxicology.
A patients ABO genetic make-up influences their susceptibility to IVIg-associated hemolysis. Having the O allele or the A2 subtype of group A blood seemed to protect against hemolysis. This may be related to antigen density - the amount of antigen on the red blood cell - which has been previously linked to the extent of hemolysis. People who are AO (or BO) have less antigen on the surface of their red blood cells than those who are AA (or BB). Group A2 is also known to have a lower antigen density than group A1.. While these findings are preliminary and limited by the small number of patients tested, they show that patients who are group A1B are at higher risk for IVIg-associated hemolysis. The results may seem to suggest that to minimize the risk of IVIg-associated hemolysis, IVIg treatment should be avoided in patients who are known to be blood group A1B. However, determining whether a patient is A1B, or indeed AA, AO, BB, or BO, requires genetic testing, which is not routinely done. Typing ...
Contusion or cortical laceration causes bleeding within the neuropil, followed by red blood cell hemolysis and deposition of hemoglobin within the neuropil. Iron, liberated from hemoglobin and transferrin and deposited as hemosiderin, is found within the brain of patients with PTE.55 Iron is critical to biological functions, but the two stable oxidation states and the redox
1. The effect of a number of aromatic polysulphonic acids on the kinetics of haemolysis of rabbit erythrocyte suspensions by crude staphylococcal α-toxin was studied at pH8·6 and 6·8. 2. All of the inhibitory compounds caused an increase in the prelytic lag time (τ) of the sigmoid haemolysis curves, an increase in the time to reach 50% haemolysis (t½) and a decrease in the maximum rate of haemolysis (Rmax.). The most inhibitory compounds caused a 50% decrease in Rmax. at concentrations between 0·1 and 0·2mm. 3. The effect of pH varied considerably: compounds (I) and (II) were almost equally inhibitory at both pH values, compounds (IV) and (IX) were more inhibitory at pH6·8 than at pH8·6, and compounds (VII), (VIII), (X), (XI) and (XII) were more inhibitory at pH8·6. 4. Increased time of premixing α-toxin with compound (I) caused increased inhibition. 5. An attempt was made, where possible, to relate the inhibitory activity to the structure of the test compound.. ...
This is a study on 62 G6PD patients hospitalized in Bahrami hospital between 1993 to 1995. Favea was the oxidant agent causing acute hemolysis in more than 90 percent of patients. Most of acute hemolysis occurred in spring, the season of high consumption of favea. More than 60 percent of patients ...
Octylphenoxy polyoxyethylene ethers (Triton detergents) interact with the erythrocyte membrane in a biphasic manner, i.e. they stabilize erythrocytes against hypo-osmotic haemolysis at low concentrations (0.0001-0.01%, v/v), but become haemolytic at higher concentrations. This biphasic behaviour was demonstrated with Triton X-114, Triton X-100 and Triton X-102. However, a critical chain length is a prerequisite for the haemolytic effect, because Triton X-45, which differs from the other Tritons only by the shorter chain of the polyoxyethylene residue, does not exhibit this biphasic behaviour, but goes on protecting against osmotic rupture up to saturating concentrations. Even a 1% solution of Triton X-45 does not cause haemolysis. This structural specificity of Triton X-45, namely the lack of haemolysis and efficient stabilization against osmolysis even at higher concentrations of the detergent, is exhibited at 0 degree and 37 degrees C as well as at room temperature. Three conclusions are ...
Definitions of hemolysis. What is hemolysis: The destruction or dissolution of red blood cells, with subsequent release of hemoglobin.. Synonyms: characte, plt, coagulation, coagulopathy, downsizing, fibrin, haematolysis, haemolysis, hematolysis, hemolyses, hemophilia, leukopenia, lysis, pruritus, sinusitis, thrombophlebitis
May be allergic or hemolytic 1. Hemolytic reactions: can be acute or delayed Acute Hemolytic reactions: It is usely due to ABO incompatibility…
c-C3BP or rGAPDH was observed (Figure 3c, d). The H.c-C3BP or rGAPDH interaction with C3 was specific and strong, which was evident from the fact that the column-bound C3 was eluted at high salt wash (0·5 m NaCl) or by lowering the pH to 2·2. To test whether H.c-C3BP or rGAPDH binding to C3 would influence complement function, a simple haemolytic assay was performed where the lysis of sensitized sheep erythrocytes by serum complement proteins was measured. As shown in Figure 3(e, f), a dose-dependent inhibition of erythrocyte lysis by H.c-C3BP and rGAPDH was observed. To rule out that the observed inhibition was not due to suppression of the classical pathway, binding of C1q protein by H.c-C3BP was. measured. No interaction among these proteins was evident in the microtitre plate assay (not shown). To confirm check details whether the inhibition of erythrocyte lysis by H.c-C3BP or rGAPDH was due to suppression of C3 activation, the formation of membrane attack complex (MAC) was measured on the ...
Syringomycin is a necrosis-inducing lipopeptide toxin synthesized and secreted by the phytopathogen, Pseudomonas syringae pv. syringae. Although small quantities of syringomycin are known to activate a cascade of physiological events in plasma membranes, the mechanism of action of the phytotoxin has never been fully characterized. The objective of this study was to test the hypothesis that the primary mode of action of syringomycin is to form transmembrane pores that are permeable to cations. Accordingly, direct measurement of ion fluxes were performed using artificial bilayers. The hemolytic properties and surface activity of HPLC-purified syringomycin were quanlified by use of an erythrocyte lysis assay and by the drop weight method. Assays were performed using syringomycin form SRE alone or a mixture containing all forms of the phytotoxin. At a threshold concentration of 500 ng/ml, syringomycin induced hemolysis by forming ion channels in membranes. Osmotic protection studies indicated a ...
A transient decrease in hemoglobin is to be expected following IV RhIG therapy in D-positive patients. In a large study of 272 patients,3 hemolysis wa...
Purchase your copy of ASTM F756 - 17 as a PDF download or hard copy directly from the official BSI Shop. All BSI British Standards available online in electronic and print formats.
A normal test result is called a negative result. A normal result shows that less than 5% of red blood cells break down when tested. This breakdown is called hemolysis.. A negative test does not rule out PNH. False-negative results may occur if the fluid part of blood (serum) lacks complement. Normal value ranges may vary slightly among different laboratories. Some labs use different measurements or test different samples. Talk to your provider about the meaning of your specific test results. ...
TY - JOUR. T1 - Biological and environmental factors affecting ultrasound-induced hemolysis in vitro. T2 - 1. HIV macrocytosis (cell size). AU - Miller, Morton W.. AU - Luque, Amneris E.. AU - Battaglia, Linda F.. AU - Mazza, Salvatore. AU - Everbach, E. Carr. PY - 2003/1/1. Y1 - 2003/1/1. N2 - This paper reports the results of a further test of the hypothesis that the extent of ultrasound (US)-induced cell lysis in the presence of a US contrast agent to enhance cavitational effects is a function of cell size. The present data support the hypothesis. Human adult erythrocytes in vitro derived from patients with HIV (n = 15) and apparently healthy individuals (n = 15) were compared for US-induced hemolysis in vitro. The anticoagulated whole blood from patients with HIV and macrocytic erythrocytes had significantly greater (p ,0.0001) mean corpuscular volume (MCV) and a significantly greater (p ,0.03) extent of US-induced hemolysis in vitro relative to blood from apparently normal, healthy ...
Red blood cells vecome haemolysed in solutions which have a high water potential, for example when they are placed ina solution which has a lower concentration than the blood plasma as unlike plant cells, they dont have a cell wall. A cell wall in planr cells allows the cell to beome turgid, whereas in red blood cells, the cell bursts when it is too full of water. Hope this helps ...
figure 2 from frequency and causes of lipemia interference, evidence based validation of hemolysis index thresholds by, plots the relationship between hemolysis and lipemic index, laboratory diagnosis of thalassemia brancaleoni 2016, evidence based validation of hemolysis index thresholds by
Semantic Scholar is a free, AI-powered research tool for scientific literature, based at the Allen Institute for AI.. Learn More → ...
The Hemolysis Agent For Blood Cell Analyzer market is expected to grow from USD X.X million in 2020 to USD X.X million by 2026, at a CAGR of X.X% during the forecast period. The global Hemolysis Agent For Blood Cell Analyzer market report is a compre...
Most people chose this as the best definition of haemolysis: Alternative spelling of h... See the dictionary meaning, pronunciation, and sentence examples.
In the last few years the spoilage of vacuum-packed, refrigerated beef due to psychrophilic and psychrotolerant clostridia, including production of gas inside the pack, has gained in importance. In addition to C. estertheticum and C. gasigenes, further psychrophilic clostridia have been described, which are able to cause Blown Pack Spoilage. Because there are divergent descriptions of these spoilage-causing bacteria, the phenotypic characteristics of the reference strains of six psychrotolerant clostridia were examined and the results were compared. In doing so, dissent results have been detected especially concerning hemolysis on blood agar plates as well as size of the vegeta- tive cells. The examination of C. gasigenes showed that there are distinct transverse pro- cesses in the last third of the rods of C. gasigenes, which, at present state of knowledge, have not been mentioned in the literature yet. The intention of this study was, to get more findings about the occurrence and the ...
Cone Bioproducts Red Blood Cell Matrix is freshly produced from donor red blood cells, and suitable for quality control and calibrator applications such as immunosuppressants. It serves as an excellent additive for hemoglobin adjustment and is regularly analogous for whole blood base matrices. Final filtration all the way through to 0.22um pore size is available, and custom requirements for sourcing may be met upon request.. ...
Need help with your Microbiology homework? Learners examine microscope images of blood agar and the various types of hemolysis that can be detected. A quiz completes the activity.
Hemolysis is a breakdown of the bodys red blood cells, typically as a result of disease or taking certain medications. It leads...
hemolysis definition: The destruction or dissolution of red bloodstream cells, with subsequent release of hemoglobin.; The destruction of red bloodstream cells, and subsequent release of hemoglobin, on…
TY - JOUR. T1 - Delayed hemolysis. AU - Sutor, Laurie J.. PY - 2002. Y1 - 2002. UR - UR - M3 - Article. AN - SCOPUS:0036146739. VL - 33. SP - 148. EP - 149. JO - Laboratory Medicine. JF - Laboratory Medicine. SN - 0007-5027. IS - 2. ER - ... In Vitro and In Vivo Hemolysis Giuseppe Lippi (Author) Hardcover: 200 pages Publisher: De_Gruyter; 1 edition (July 16, 2012) ISBN-10: 3110246139
β-hemolytic activity of the OKB105 cells compared to NHY1, the ybdT mutant. Blood agar plate was streaked with a single colony of OKB105 (wild-type) or NHY1 (y
MiTiHeart® LVAD - Small Rotary Centrifugal Blood Pump: 0.64 kg, 5 cm Diameter, 8 cm long - Low Power Magnetic Levitation System - Low Hemolysis at Wide Range of Flow: NIH , 0.004 mg/dL ...
Collection Container/Tube: Sterile vial. Submission Container/Tube: Plastic vial. Specimen Volume: 1 mL. Collection Instructions:. 1. Obtain aliquot from second collection vial.. 2. Immediately place aliquot on ice.. Additional Information:. 1. Specimens that have not been kept refrigerated, or which have been tested for other analytes previously, may give a false-positive result.. 2. Hemolyzed specimens will give false-positive results. Specimens should be centrifuged to remove any red cells before shipping. The test will be canceled if there is any level of hemolysis present.. ...
It appears, from the investigations in other laboratories, that the anemia observed in cases of lead poisoning is due to destruction of blood rather than to diminished production of blood. The method of poisoning cells in vitro with lead was adopted in order to study this phenomenon, and distinct effects were thereby obtained, even when only 0.001 mg. of lead is added to approximately 5 billion washed red corpuscles. In order to obtain optimum results the usual dosage employed was ten times this or 0.01 mg. per 5 billion cells. The following changes were observed in cells so treated.. 1. Such a marked increase in the resistance to hypotonic salt solution develops that complete hemolysis does not occur until the cells are exposed to a saline solution of 0.05 per cent. Untreated cells are completely hemolyzed in 0.25 or 0.225 per cent saline.. 2. This reaction is quantitative and varies with the concentration of lead used. Under the conditions of our experiments this phenomenon seems to be unique. ...
StraightFrom® Whole Blood CD138 MicroBeads were developed for rapid positive selection of CD138+ cells directly from whole blood or bone marrow, thus minimizing hands-on time and maximizing yield of target cells. No sample preparation is required, including density gradient centrifugation and erythrocyte lysis. - Belgique
StraightFrom® Whole Blood CD3 MicroBeads were developed for rapid positive selection of CD3+ cells directly from whole blood or bone marrow. No sample preparation is required, including density gradient centrifugation or erythrocyte lysis. - Australia
By blocking the complement cascade with eculizumab, clinically evident intravascular hemolysis in PNH is abolished. 8 In this study, we tested for the presence of C3 fragments on the surface of PNH red cells as a possible mechanism for the residual hemolysis occurring in some eculizumab-treated patients. We observed increased levels of C3 fragments on the surface of PNH red cells in many patients who were receiving eculizumab, as also recently reported by Risitano et al.19 The deposition of C3 fragments on PNH red blood cells in this setting could represent the revelation of a pathway of red cell clearance in PNH which is usually obscured by the rapidity of intravascular hemolysis in the absence of eculizumab therapy. Indeed, a small proportion of red cells from a patient with PNH not treated with eculizumab was shown by flow cytometric analysis to have C3 deposition that was undetectable by DAT. This is likely due to the lack of sensitivity of the DAT assay and demonstrates that low levels of ...
Acute Cytomegalovirus (CMV) infection seldom presents with hemolytic anemia in an immunocompetent host. When hemolysis does occur, it is typically immune mediated and extravascular, but can be intravascular with the degree of anemia ranging from mild to severe. We report a case of a healthy 27-year-old woman who presented with jaundice and non-immune mediated intravascular hemolysis as an initial feature of acute CMV infection. Viral etiologies (including CMV) should be considered in the differential diagnosis of patients with Coombs negative intravascular hemolysis.
A total of 80 Candida isolates representing 14 species were examined for their respective responses to an in vitro hemolytic test. A modification of a previously described plate assay system where the yeasts are incubated on glucose (3%)-enriched sheep blood agar in a carbon dioxide (5%)-rich environment for 48 h was used to evaluate the hemolytic activity. A group of eight Candida species which included Candida albicans (15 isolates), C. dubliniensis (2), C. kefyr (2), C. krusei (4), C. zeylanoides (1), C. glabrata (34), C. tropicalis (5), and C. lusitaniae (2) demonstrated both alpha and beta hemolysis at 48 h postinoculation. Only alpha hemolysis was detectable in four Candida species, viz., C. famata (3), C. guilliermondii (4), C. rugosa (1), and C. utilis (1), while C. parapsilosis (5) and C. pelliculosa (1) failed to demonstrate any hemolytic activity after incubation for 48 h or longer. This is the first study to demonstrate the variable expression profiles of hemolysins by different ...
Enhancement of the CAMP reaction by heated milk whey and maltose when incorporated into a selective agar medium, the thallium crystal violet toxin medium (TKT), is described. Milk whey was prepared by enzyme coagulation of milk at room temperature or by acid coagulation followed by neutralization with sodium hydroxide. Concentrations of whey from 1-14% (v/v) were examined. The CAMP reaction increased with increasing whey concentration. A similar increase in the CAMP reaction was also observed with increasing concentration of maltose up to 1% (w/v), beyond which it decreased with increasing concentration of maltose. A combination of heated milk whey with maltose produced a CAMP reaction in excess of that produced by whey or maltose alone ...
Preanalytical phase components are the leading causes of poor sample quality, wherein inappropriate or mishandled procedures for collecting blood specimens may be associated with a magnified risk of unsuitable samples (1,2). Among various preanalytical non-conformances that can be encountered in routine laboratory practice, sample hemolysis represents the primary source of problems, in terms of prevalence and likelihood of sample rejection (3,4). Spurious hemolysis, also referred to as in vitro hemolysis, is conventionally defined as erythrocyte injury or breakdown occurring during or after sample collection, once potential sources of hemolytic anemia have been ruled out. Although the observed frequency of spuriously hemolyzed samples varies widely throughout different healthcare settings, it can be estimated around ~3% of all serum or plasma samples referred to central laboratories for routine or stat testing (5). It is also noteworthy that the vast majority of hemolyzed specimens come from ...
Hemolytic anemia after treatment of severe malaria with intravenous artesunate has been described in malaria-endemic and non-malaria-endemic countries. However, evidence of hemolytic anemia after treatment of malaria with oral ACTs is limited to 2 case reports. Data from the current prospective study confirm our hypothesis that delayed posttreatment hemolysis also occurs after oral artemisinin treatment and provide insight into its frequency and clinical course. In 40% of the patients in our study with uncomplicated malaria and oral ACT treatment, laboratory signs of hemolysis were detected 2 weeks after therapy. In 5 patients, hemolysis persisted 1 month after treatment. Patients with posttreatment hemolysis showed a larger decrease in Hb levels after treatment than did patients without hemolysis. The intensity of hemolysis was mild compared with that after intravenous artesunate. In many reported cases of PADH after intravenous artesunate, patients received blood transfusions (2,9). In other ...
SGHERZA, N., Dalfino, L., Palma, A., Vitucci, A., Campanale, D., Grasso, S., & Musto, P. (2020). Hemolysis, or not Hemolysis, that is the question. Use of hydroxychloroquine in a patient with COVID-19 infection and G6PD deficiency.: Hemolysis by Hemolysis and G6PD deficiency. Mediterranean Journal of Hematology and Infectious Diseases, 12(1), e2020076. ...
METHODS: Haemolysis and potassium levels were compared before and after the use of the three different infusion pumps. Using 135 units of packed red blood cells (RBCs) aged from 10 to 28 days, 27 measurements were taken for each pump at various flow rates (30, 60, 150, 300 and 450 ml/h) and were compared with measurements taken before using the pumps. The range of flow rates was chosen to cover those of paediatric and adult transfusions.. RESULTS: The shuttle- and piston-type pumps resulted in low haemolysis levels. The peristaltic-type pump produced significantly more haemolysis, which worsened at low flow rates, but the absolute value of haemolysis remained within the range recommended by the regulatory agencies in North America and Europe. Approximately two-thirds of the haemolysis produced by the peristaltic-type pump seemed to be secondary to the use of an antisiphon valve (ASV) on the transfusion line recommended by the manufacturer. Potassium levels did not increase with the use of the ...
Stonustoxin (SNTX), a lethal factor purified from the venom of stonefish Synanceja horrida, is a protein (148,000 mol. wt) existing as a dimer comprising two subunits (α and β) of mol. wts 71,000 and 79,000, respectively. Its LD50 (i.v.) is 17 ng/g in mice and it causes haemolysis of rat and rabbit erythrocytes in vitro. Eight monoclonal antibodies (Mabs) against SNTX have been developed using the Balb/C mouse. These Mabs have been purified by Protein G affinity membrane disc chromatography. They were all classified as IgG, with half of them having κ and the rest λ light chains. They had affinity constants ranging from 3.75 x 10-9 to 9.74 x 10-9 M. Six were able to protect mice from a challenge of a lethal dose of SNTX. However, not all protective Mabs were able to neutralize the haemolytic effect in vitro. Only four Mabs (31A, 32B, 38A and 46A) could inhibit rat and rabbit erythrocyte haemolysis, while one Mab (43D) offered partial inhibition and another Mab (8A) did not inhibit haemolysis ...
Index[201 50]/Info 200 0 R/Length 113/Prev 788114/Root 202 0 R/Size 251/Type/XRef/W[1 3 1]>>stream CULTURE REQUIREMENTS OF SALMONELLA TYPHI (S. TYPHI) Columbia Horse Blood Agar medium. Fair growth; pink-colored colonies without a sheen. After sterilization, liquefy agar medium by warming in a water bath at 50 o; Add 0.5-10 ml of defribinated blood to the nutrient agar media and mix thoroughly by rotation or inversion of the flask containing the prepared medium. in diameter, colorless, lenticular with either an entire or a finely, radially striated edge and a beaten copper surface. When grown on blood agar (BA) plates, three different types of RBC lysis can be discerned: alpha (a), beta (b), or gamma (g) (Fig. Volume 7, Issue 4, 1990, Pages 295-304, ISSN 0740-0020. These throat pathogens are often Gram positive cocci that may be hemolytic, producing exotoxins called … In clinical settings, this might also include several stabs of the inoculum into the agar to encourage any anaerobic versions ...
It can also be used for the differentiation of bacteria based type of hemolysis (α-, β- or γ-hemolytic) they produce on the agar. Neisseria. NEISSERIA - CHOCOLATE AGAR CULTURE Chocolate agar (CHOC) or chocolate blood agar (CBA) - is a non-selective, enriched growth medium used for isolation of pathogenic bacteria. It helps detect Streptococcus pyogenes and at the same time helps differentiate the type of hemolysis produced such as alpha-hemolysis, beta-hemolysis, and gamma-hemolysis. Neisseria meningitidis: Surface structures, perhaps pili, facilitate attachment to mucosal epithelial cells and invasion to the submucosa. Clinical significance This causes diseases in an opportunistic fashion. ... A gram-negative, oxidase-positive diplococcus growing on chocolate agar likely belongs to the genus-Neisseria.-Mycobacterium.-Pseudomonas.-Escherichia. Blood agar and chocolate medium (blood heated at 176-194°F/80-90°C) are suitable growth media. Viridans streptococci on blood agar plate. The ...
Ensure adequate hydration by starting intravenous fluids. Oxygenation should be checked and supplemental oxygen given if needed. Because of possible severe hemolysis, adequate hydration with alkaline solutions to keep a brisk urine flow is essential. Low-dose dopamine may help preserve renal blood flow. Monitor serial CBC, BUN, creatinine, electrolytes and urinalysis including onsite dipstick of urine for hemoglobin. Administer blood in patients with hemolysis. If major hemolysis has occurred, exchange transfusion may be performed to remove the plasma hemoglobin, in conjunction with hemodialysis to preserve renal function. Recovery is dependent on the supportive care provided, the extent of the exposure and the resulting effects of hemolysis. For more poison prevention and first aid information, call the Poison Control Center toll-free, 1-800-222-1222. ...
TRALI most common cause of transfusion related mortality (Miller, 7th ed).. Acute hemolytic reaction. Definition: When antibodies to a particular blood antigen already exist. Can be delayed if there is an amnestic response to a transfused RBC antigen to which the recipient is already sensitized.. Causes: Usually the result of ABO incompatibility and technical errors made during the collection of blood.. Risks: The risk of acute hemolytic transfusion reaction due to incompatible blood is 1:4 per 1 million units transfused and has a high fatality rate.. Most of the transfused cells are destroyed which can activate the coagulation system with DIC and release of anaphylotoxins and other vasoactive amines. Patients might present with back pain, pain at site of transfusion, headache, change in vital signs, pulmonary edema, bleeding, new/worsening renal failure.. ...
Paroxysmal nocturnal hemoglobinuria (PNH) is an acquired hematopoietic stem cell disorder.12 The most common clinical manifestations of PNH include intravascular hemolysis, venous thrombosis, bone marrow failure, and occasional transition to a myelodysplastic syndrome or acute leukemia. The main biochemical feature in PNH is the absence of glycosyl-phosphatidylinositol (GPI)-anchored proteins on the surface of the affected cells, because of incomplete bioassembly of the GPI anchor affixing each protein to the cell surface. The lack of GPI-anchored complement regulatory proteins, including CD55 but especially CD59, results in complement-mediated hemolysis and hemoglobinuria, which is a major clinical manifestation of PNH and an important cause of morbidity and mortality (see Figures 2A and 2B). A humanized monoclonal anti-C5 antibody (eculizumab) that inhibits terminal complement protein activation has recently been approved for PNH in the US and Europe,13-15 suggesting that blocking complement ...
Some of the functions of cortisol such as its gluconeogenesis effects on carbohydrates and fats and the ability to counteract stress, inflammation and promote healing relate to Kidney Yin functions. Other aspects, however, actually create a wasting or yin deficiency which manifests as .
Five peptide sequences corresponding to the E1 protein of GBV-C [NCCAPEDIGFCLEGGCLV (P7), APEDIGFCLEGGCLVALG (P8), FCLEGGCLVALGCTICTD (P10), QAGLAVRPGKSAAQLVGE (P18), and AQLVGELGSLYGPLSVSA (P22)] were synthesized because they were capable of interfering with the HIV-1 fusion peptide (HIV-1 FP)-vesicle interaction. In this work the interaction of these peptides with the HIV-1 FP, as well as with membrane models, was analyzed to corroborate their inhibition ability and to understand if the interaction with the fusion peptide takes place in solution or at the membrane level. Several studies were carried out on aggregation and membrane fusion, surface Plasmon resonance, and conformational analysis by circular dichroism. Moreover, in vitro toxicity assays, including cytotoxicity studies in 3T3 fibroblasts and hemolysis assays in human red blood cells, were performed to evaluate if these peptides could be potentially used in anti-HIV-1 therapy. Results show that P10 is not capable of inhibiting ...
From a cosmid gene bank of Bacillus cereus GP4 in Escherichia coli we isolated clones which, after several days of incubation, formed hemolysis zones on erythrocyte agar plates. These clones contained recombinant cosmids with B. cereus DNA insertions of varying lengths which shared some common restriction fragments. The smallest insertionwas recloned as aPstl fragment into pJKK3-1, a shuttle vector which repücates in Bacillus subtilis and E. coli. When this recombinant plasmid (pJKK3-1 hly-1) was transformed into E. coli, it caused hemolysis on erythrocyte agar plates, but in liquid assays no extemal or intemal hemolytic activity could be detected with the E. coli transformants. B. subtilis carrying the same plasmid exhibited hemolytic activity at Ievels comparable to those ofthe B. cereus donor strain. The hemolysin produced in B. subtilis seemed to be indistinguishable from cereolysin in its sensitivity to cholesterol, activation by dithiothreitol, and inactivation by antibodies raised ...
The rate of hemolysis in sickle cell disease correlates with the quantity of active von Willebrand factor in the plasma.: Vaso-occlusion, hemolysis, and oxidati
Exhibits stronger antimicrobial activity against the Gram-positive bacteria (S.aureus (IC(50) is 7.4 ug/ml)) and fungi (C.albicans (IC(50) is 3.0 ug/ml) and P.pastoris (IC(50) is 0.1 ug/ml)) than Gram-negative bacteria (E.coli no inhibition at 100 ug/ml). Binds to chitin (4.3 uM are required to obtain 50% of binding). Does not cause hemolysis on sheep erythrocytes. Has no blocking activity on the P-type calcium channel.
Clin Perinatol 31 (2004) 807-833 Diagnosis and management of hemolysis, elevated liver enzymes, and low platelets syndrome John R. Barton, MDa,b,*, Baha M. Sibai, MDb aCentral…
The answer is C. Anaphylaxis, dyspnea, urticarial, and diarrhea can all be seen with a type A anaphylaxis reaction. Hemolysis can be secondary to either physical damage to erythrocytes due to a narrowing or blockage of the blood passages, including the dialysis tubing, catheter or needle or to issues with the dialysis solution. Hemolysis can be due to hypotonic dialysis solutions, solutions contaminated by chemicals in the water supply that have not been removed during the preparation of dialysate. ...
Haptoglobin is very sensitive but not specific for clinically significant hemolysis, as even small amounts of free hemoglobin can deplete normal levels of serum haptoglobin. It may also be decreased in liver disease. As haptoglobin is an acute phase reactant, it may be elevated in a number of diseases leading to difficulties in interpretation.. LDH is present in all tissues, including red cells, so elevations are consistent with hemolysis but very nonspecific, as any cellular damage may affect levels. As such, there are many other disorders that may cause an elevated LDH.. Other disorders may be associated with basophilic stippling.. ...
Nov/02/2011. SARASOTA (Nov. 2, 2011) - Becton, Dickinson and Company (BD) presented Sarasota Memorial Health Care System with its first ever BD Quality Award today for pioneering best practices in blood collection techniques that promote safe, high quality patient care.. The award - presented by the leading global medical technology company BD - recognized Sarasota Memorial for developing state-of-the-art processes and procedures designed to prevent hemolysis in patient blood samples sent to the clinical laboratory for testing. Hemolysis is the breakdown of red blood cells. If significant hemolysis occurs to a sample, it could affect test results and lead to additional blood draws and tests.. In its continuing effort to develop best practices in patient care, Sarasota Memorial worked with BD to enhance and standardize its new blood collection protocol, educate its clinical staff and put in place a quality control program that ensures the best techniques and proper blood collection products are ...
Chronic haemolysis places continuous strain on vital organs, particularly the liver; kidneys; and heart, thus increasing the risk of serious and life-threatening complications such as liver and/or kidney failure; heart attack; stroke; deep vein thrombosis; Budd-Chiari Syndrome (a clot or clots in the hepatic vein of the liver); or blood clots in other veins or organs.. Clots are the most common complication of PNH and the leading cause of death among patients. This is a scary thing for a patient to face, but knowing the signs and symptoms of a clot and getting treatment as soon as possible can minimise the risk of further complication. If you experience swelling in the limbs or abdomen and severe and/or sudden onset of pain, you should seek medical advice immediately.. ...
TY - JOUR. T1 - Genetic determinants of haemolysis in sickle cell anaemia. AU - Milton, Jacqueline N.. AU - Rooks, Helen. AU - Drasar, Emma. AU - McCabe, Elizabeth L.. AU - Baldwin, Clinton T.. AU - Melista, Efi. AU - Gordeuk, Victor R.. AU - Nouraie, Mehdi. AU - Kato, Gregory R.. AU - Minniti, Caterina. AU - Taylor, James. AU - Campbell, Andrew. AU - Luchtman-Jones, Lori. AU - Rana, Sohail. AU - Castro, Oswaldo. AU - Zhang, Yingze. AU - Thein, Swee Lay. AU - Sebastiani, Paola. AU - Gladwin, Mark T.. AU - Badesch, D. B.. AU - Barst, R. J.. AU - Castro, O. L.. AU - Gibbs, J. S.R.. AU - Girgis, R. E.. AU - Goldsmith, J. C.. AU - Hassell, K. L.. AU - Kato, G. J.. AU - Krishnamurti, L.. AU - Lanzkron, S.. AU - Little, J. A.. AU - Machado, R. F.. AU - Morris, C. R.. AU - Onyekwere, O.. AU - Rosenzweig, E. B.. AU - Sachdev, V.. AU - Schraufnagel, D. E.. AU - Waclawiw, M. A.. AU - Woolson, R.. AU - Yovetich, N. A.. AU - Steinberg, Martin H.. PY - 2013/4/1. Y1 - 2013/4/1. N2 - Haemolytic anaemia is ...
Check out The Kaji Review from WikEM Press, with over 750 emergency medicine study questions to help you ace that next test and keep up with the latest evidence! ...
TY - JOUR. T1 - VARIAZIONI DI PRESSIONE COLLOIDO-OSMOTICA DOPO CHIRURGIA MAGGIORE. AU - Sghirlanzoni, S.. AU - Geraci, P.. AU - Servadio, G.. PY - 1978. Y1 - 1978. N2 - The Authors considered 12 patients submitted to surgery of different durations and took their colloid-osmotic pressure (COP) by Weils oncometer at different times. With regard to the standard conditions, a statistically significant COP decrease at 16th (24.3%) and 24th (18%) hour is reported. The correlation between surgical stress and COP decrease is significant, too. Pathogenesis and therapy are discussed with emphasis to direct COP monitoring in high risk surgical patients.. AB - The Authors considered 12 patients submitted to surgery of different durations and took their colloid-osmotic pressure (COP) by Weils oncometer at different times. With regard to the standard conditions, a statistically significant COP decrease at 16th (24.3%) and 24th (18%) hour is reported. The correlation between surgical stress and COP decrease ...
Don;t understand why you wasted so much media and time with irrelevant tests. Please determine if this isolate shows alpha hemolysis on blood agar ...
Hemoglobin removal from red blood cell lysate for proteomics and biomarker drug discovery. The flow through fractions(hemoglobin depleted) retain their enzymatic and biological activity
CPPs have for numerous years been utilized as delivery vectors of various pharmaceutically interesting cargoes, both in vitro and in vivo. As CPPs are gradually approaching the bedsides, investigating toxicity associated with these highly interesting peptides becomes increasingly important and thorough initial assessment of cytotoxicity in vitro is a first step towards advancing these delivery vehicles in to the clinics. The present chapter describes protocols for four cytotoxicity assays in order to provide a toolbox for toxicity assessment of CPPs. The foci lie on membrane integrity (deoxyglucose leakage and propidium iodide assays) and cell viability (the MTT assay), but the chapter also provides a protocol for assessing an important parameter for future clinical applications, namely the hemolytic properties of CPPs.
Fingerprint Dive into the research topics of Association of G6PD,sup,202A,376G,/sup, with lower haemoglobin concentration but not increased haemolysis in patients with sickle cell anaemia. Together they form a unique fingerprint. ...
Cerebroside sulfate (CGS) was found to be capable of inhibiting complement-dependent hemolysis. The activity dependence of CGS-containing liposomes on their com
PNH clone size and the development of intravascular hemolysis , assessed by LDH values (Pearson Correlation Coefficient=0.80, ... granulocyte PNH size of 23% were the thresholds to predict hemolysis as measured by an elevated LDH (ROC analyses with AUC=0.96 for .... Research Article last updated 10/11/2011 - 5:58pm.. ...
Modular program-based one-time assessment of hemolysis events following new use of several immunoglobulin products, including Flebogamma, Gammagard liquid, Gammaplex, Gamunex, Octagam, Privigen, Lyophilized product IV, Non-lyophilized intramuscular, Non-lyophilized unspecified route, and other intravenous immunoglobulin (IVIg) products. The query was run against the Mini-Sentinel Distributed Database for the time period of January 1, 2006- December 31, 2012. Modular programs are adaptable standardized programs. Results were generated using Modular Program 6, version 6.0; click here for more information. The queries were distributed to 18 Data Partners in October 2013. If you are using a web page screen reader and are unable to access this document, please contact the Mini-Sentinel Operations Center for assistance by clicking on the Submit Comments link above or sending an email requesting assistance to [email protected] ...
772. When infusing blood, hemolysis is a risk when small guage needles are used and if the infusion rate exceeds what? / Indications for FFP / Indications for platelets and contraindication / One unit of pRBCs is expected to raise the Hb/HCT by how much in an average adult? / What is the volume of one unit of pRBCs ...
213 during the first 24 hours after injury showed no elevation which could be attributed either to shock or to infusion of bank blood. The mean plasma hemoglobin level for the entire group examined within 24 hours of injury was 9.6 mg. per 100 cc. of plasma, a normal figure for the technique used (See Materials and Methods). The mean for the moderate-and-severe shock group (8.8) was actually lower than the mean (11.7) of the minimal-shock group. More surprising are comparisons based on the quantity of transfused blood. The plasma hemoglobin levels actually appear to decline from a mean of 11.1 mg. per 100 cc. in the 14 patients who had received no blood to 9.2 in 22 patients who had had from 1 to 3 transfusions, and a minimum level of 7.5 in the 5 patients who had received the largest quantities of blood--from 4 to 14 units. These apparently paradoxical differences are not statistically significant, but it is clear that in this study no evidence emerged to indicate that during the first 24 hours ...
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As an exception, an autosomal recessive inherited GPI deficiency resulting from a mutation of the PIG-M gene has been recently described in two kindreds; however, the phenotype was clearly different from that of PNH, with a partial GPI deficiency resulting in a clinical syndrome characterized by propensity to thrombosis and seizures, in the absence of significant hemolysis (Almeida et al 2006; Almeida et al 2007 ...
In limited case reports, intentional oral ingestions of formic acid have resulted in life-threatening metabolic acidosis, profound hypotension, shock, ARDS, acute renal failure, and severe hemolysis. Systemic effects have also been reported following dermal exposure ...
Hemolysis, Icterus and Lipemia (HIL) are the most commonly tested interferences that affect the integrity of patient samples in the clinical laboratory. Depending on the type and the degree of interferences, it may impact patient results when run with colorimetric assays. Fortunately, most major chemistry platforms include automated HIL detection feature that employs spectrophotometric detection to measure the level of common interferences. Liquichek Serum Indices is intended for use as part of laboratory interference testing to monitor the ability of the instrument to detect Hemolyzed, Icteris or Lipemic (HIL) samples.. ...
A. Rh incompatibility: Rh antigens from the babys blood enter the maternal bloodstream. The mothers blood does not contain Rh factor, so she produces anti-Rh antibodies. These antibodies are harmless to the mother but attach to the erythrocytes in the fetus and cause hemolysis. Exchange of fetal and maternal blood takes place primarily when the placenta separates at birth.. ...
Hemolysis[edit]. In medicine, LDH is often used as a marker of tissue breakdown as LDH is abundant in red blood cells and can ... Other uses are assessment of tissue breakdown in general; this is possible when there are no other indicators of hemolysis. It ... Tissue breakdown releases LDH, and therefore LDH can be measured as a surrogate for tissue breakdown, e.g. hemolysis. LDH is ... function as a marker for hemolysis. A blood sample that has been handled incorrectly can show false-positively high levels of ...
"Intravascular hemolysis". eClinpath. Retrieved 2019-05-08. "Bilirubin and hemolytic anemia". eClinpath. Retrieved 2019-05-08. ... concentrations are low or depleted as a result of severe or prolonged hemolysis. Both Hp and Hx are acute-phase proteins, the ...
Thus, the haptoglobin will stay normal if the hemolysis is not severe. In severe extra-vascular hemolysis, haptoglobin levels ... haptoglobin levels will be decreased in case of intravascular hemolysis or severe extravascular hemolysis. In the process of ... "HEMOLYSIS AND JAUNDICE: An overview" (PDF). Retrieved 9 July 2011. Graversen JH, Madsen M, Moestrup SK (2002). "CD163: a signal ... In intravascular hemolysis, free hemoglobin will be released into circulation and hence haptoglobin will bind the hemoglobin. ...
... there are two principal mechanisms of hemolysis; intravascular and extravascular. Intravascular hemolysis describes hemolysis ... Extravascular hemolysis refers to hemolysis taking place in the liver, spleen, bone marrow, and lymph nodes. In this case ... Chronic hemolysis leads to an increased excretion of bilirubin into the biliary tract, which in turn may lead to gallstones. ... Intravascular hemolysis may occur when red blood cells are targeted by autoantibodies, leading to complement fixation, or by ...
Haemolysis is reported. It has also been reported to cause post kidney failure in children. Like other antibiotics, ceftriaxone ... Guleria VS, Sharma N, Amitabh S, Nair V (September-October 2013). "Ceftriaxone-induced hemolysis". Indian Journal of ...
"What is Hemolysis?" (PDF). Becton-Dickinson. Archived (PDF) from the original on 2008-06-25. Retrieved 2008-06-01. Akerblom O, ...
"Microbiology Primer: Hemolysis". Archived from the original on 2008-12-11. Retrieved 2008-12-12. "Streptococcaceae Answers". ... that enlarges the area of hemolysis formed by the β-hemolysin elaborated from Staphylococcus aureus. Although the test is ...
Neter, E (1956). "Bacterial Hemagglutination and Hemolysis". Statler Research Laboratories and Department of Pediatrics, ...
... hemolysis can also occur. MD is not persistent, meaning that it will dissipate after a short time. It is, however, still quite ...
Stalnikowicz R, Amitai Y, Bentur Y (2004). "Aphrodisiac drug-induced hemolysis". Journal of Toxicology. Clinical Toxicology. 42 ...
Rao, D. Sheshagiri; Barik, Ramachandra; Siva Prasad, Akula (1 September 2016). "Hemolysis induced by PMIVSD occluder". Indian ...
Crenellation Cytorrhysis Hemolysis Plasmolysis "Crenate". Oxford Dictionaries. Stoker, HS (2012). General, Organic, and ...
Can occur hemolysis and erythrocytes. It occurs mostly in moist soil in shade, at the height of 1000-3000 m as climbs over ...
No hemolysis has been observed. Growth has been documented on trypticase soy agar, but the size of the colonies are roughly ...
Histophilus does not exhibit consistent hemolysis. Their ability to reduce nitrogen allows them to be facultative anaerobes and ...
... display alpha-hemolysis. Alpha-hemolysis is also termed incomplete hemolysis or partial hemolysis because the cell membranes of ... Beta hemolysis (β-hemolysis), sometimes called complete hemolysis, is a complete lysis of red cells in the media around and ... Streptococcus pyogenes, or GAS, displays beta hemolysis. Some weakly beta-hemolytic species cause intense hemolysis when grown ... When alpha-hemolysis (α-hemolysis) is present, the agar under the colony will appear dark and greenish due to the conversion of ...
Hemolysis leads to elevated bilirubin levels. After delivery bilirubin is no longer cleared (via the placenta) from the ... Acute hemolytic transfusion reactions due to immune hemolysis may occur in patients who have no antibodies detectable by ... In the presence of significant hemolysis the smear will show schistocytes (fragmented red blood cells), reticulocytosis, and in ... hemolysis). This is a major cause of HDN, because 75% of pregnancies result in some contact between fetal and maternal blood, ...
"Immune Hemolysis: Diagnosis and Treatment Recommendations". Seminars in Hematology. Anemia in Clinical Practice. 52 (4): 304- ... "Drug-induced hemolysis: Cefotetan-dependent hemolytic anemia mimicking an acute intravascular immune transfusion reaction". ... Quinidine Penicillin in high doses can induce immune mediated hemolysis via the hapten mechanism in which antibodies are ...
Shirron, N; Korem, M; Shuster, A; Leikin-Frankel, A; Rosenberg, M (2008). "Effect of Alcohol on Bacterial Hemolysis". Curr. ... Shuster, A; Korem, M; Jacob-Hirsch, J; Amariglio, N; Rechavi, G; Rosenberg, M (2011). "Microbial alcohol-conferred hemolysis is ...
α-Hemolysis will only cause partial lysis of the red blood cells (the cell membrane is left intact) and will appear green or ... γ-Hemolysis (or nonhemolytic) is the term referring to a lack of hemolytic activity. BAPs also contain meat extract, tryptone, ... "Blood Agar Plates and Hemolysis Protocols". Archived from the original on 2012-02-02. Retrieved 2014-10-28. Fisher, Bruce; ...
Growth on rabbit blood shows slight hemolysis. In glucose broth, the cultures appear turbid with a smooth sediment and a final ...
Engel, Rolf R.; Rodkey, F. Lee; Krill, Carl E. (April 1, 1971). "Carboxyhemoglobin Levels as an Index of Hemolysis". Pediatrics ...
Sharp MK, Mohammad SF (Sep 1998). "Scaling of hemolysis in needles and catheters". Annals of Biomedical Engineering. 26 (5): ... and 25G butterflies connected directly to vacuum tubes caused the same amount of hemolysis and gave the same coagulation panel ... that shear stress and hence hemolysis decrease with decreasing needle bore (but the decrease can be clinically insignificant). ...
Hemolysis, magnesium concentration in red blood cells is approximately three times greater than in serum, therefore hemolysis ... Hypermagnesemia is expected only in massive hemolysis. Chronic kidney disease, excretion of magnesium becomes impaired when ...
... mechanisms of hemolysis, red blood cell destruction; and iron overload, a serious chronic condition in which the body absorbs ...
Melrose, W. D.; Bell, P. A.; Jupe, D. M.; Baikie, M. J. (1990-01-01). "Alcohol-associated haemolysis in Zieve's syndrome: a ... ISBN 0-19-262515-2. Shukla, Sandhya; Sitrin, Michael (2015-07-09). "Hemolysis in Acute Alcoholic Hepatitis: Zieve's Syndrome". ...
... s also cause hemolysis of mammalian blood cells. The main purpose of the secretion of grammastin is defensive and ... Grammistins affect organisms by cytolysis and hemolysis. As well as being toxic they are also antibiotic and antimicrobial. " ...
... a clue to footstrike hemolysis. Runner's anemia as a benefit versus runner's hemolysis as a detriment". The American Journal of ... The impact forces from running can lead to red blood cell hemolysis and accelerate red blood cell production. This can shift ... Repetitive impacts to the body may cause mechanical trauma and bursting (hemolysis) of red blood cells. This has been ... "Rust Urine after Intense Hand Drumming Is Caused by Extracorpuscular Hemolysis". Clinical Journal of the American Society of ...
Hemolysis on blood agar is beta-hemolytic. It ferments D-glucose, lactose, maltose, sucrose, salicin, D-sorbitol, and starch, ...
It exhibits β-hemolysis activity, thus explaining its growth on chocolate or blood agar, but must be supplemented with NAD ('V ... Severe hemolysis or hemorrhaging is also present. Several virulence factors account for the remarkable pathogenicity of A. ...
Lipidemia in the Setting of Hemolysis. Dinesh Pradhan, Qian Sun, Octavia M. Peck Palmer ... Propofol and its lipid emulsion solvent, required for the drugs solubilization, cause in vivo hyperlipidemia and hemolysis (2 ...
Hemolysis is the breakdown of red blood cells. ... Conditions that can cause hemolysis include:. *Immune reactions ...
Successful Use of Plasma Exchange for Profound Hemolysis in a Child With Loxoscelism Ahmed Said, Paul Hmiel, Matthew Goldsmith ... Can an Alternative Umbilical Arterial Catheter Solution and Flush Regimen Decrease Iatrogenic Hemolysis While Enhancing ...
Extravascular hemolysis refers to hemolysis taking place in the liver, spleen, bone marrow, and lymph nodes. In this case ... Hemolysis or haemolysis (/hiːˈmɒlɪsɪs/), also known by several other names, is the rupturing (lysis) of red blood cells ( ... Hemolysis may occur if the centrifuge rotates too quickly (generally greater than 500 rpm)-essentially this is hemolysis ... Experience and proper technique are key for any phlebotomist, nurse or doctor to prevent hemolysis. In vitro hemolysis during ...
The sugar-water hemolysis test is a blood test to detect fragile red blood cells. It does this by testing how well they ... Sucrose hemolysis test; Hemolytic anemia sugar water hemolysis test; Paroxysmal nocturnal hemoglobinuria sugar water hemolysis ... The sugar-water hemolysis test is a blood test to detect fragile red blood cells. It does this by testing how well they ... Sucrose hemolysis test - diagnostic. In: Chernecky CC, Berger BJ, eds. Laboratory Tests and Diagnostic Procedures. 6th ed. St ...
Intravascular hemolysis describes hemolysis that happens mainly inside the vasculature. As a result, the contents of the red ... Intravascular hemolysis is the state when the red blood cell ruptures as a result of the complex of complement autoantibodies ... Schaer, D. J.; Buehler, P. W.; Alayash, A. I.; Belcher, J. D.; Vercellotti, G. M. (2012-12-20). "Hemolysis and free hemoglobin ... "Intravascular hemolysis". eClinpath. Retrieved 2019-05-08. Muller, Andre; Jacobsen, Helene; Healy, Edel; McMickan, Sinead; ...
Haemolysis in Cholera. Br Med J 1957; 2 doi: (Published 28 December 1957) Cite this ...
Helping you find trustworthy answers on Transfusion Hemolysis , Latest evidence made easy ... Find all the evidence you need on Transfusion Hemolysis via the Trip Database. ... Transfusion Hemolysis Transfusion Hemolysis Transfusion Hemolysis Toggle navigation Brain Head & Neck Chest Endocrine Abdomen ... 5. Hemolysis risk after packed red blood cells transfusion with infusion pumps (PubMed). Hemolysis risk after packed red blood ...
... , Immune Transfusion Reaction, Hemolytic Transfusion Reaction. ... Transfusion Hemolysis. Transfusion Hemolysis Aka: Transfusion Hemolysis, Immune Transfusion Reaction, Hemolytic Transfusion ... Signs: Intravascular Hemolysis (Major Incompatibility) *Rapid and massive Hemolysis to shock state ... These images are a random sampling from a Bing search on the term "Transfusion Hemolysis." Click on the image (or right click) ...
Haemolysis and liver macrophages. *Jordan Hindson. 1. Nature Reviews Gastroenterology & Hepatology volume 17, page650(2020)Cite ... Hindson, J. Haemolysis and liver macrophages. Nat Rev Gastroenterol Hepatol 17, 650 (2020). ... Hemolysis transforms liver macrophages into antiinflammatory erythrophagocytes. J. Clin. Invest. ...
Hemolysis is a breakdown of the bodys red blood cells, typically as a result of disease or taking certain medications. It ... My blood test says: hemolysis 4+ abnormal. What does the 4+ mean? Post your comments. Please enter the following code: ... Extensive blood testing can determine whether a person has hemolysis. There are a variety of conditions which may contribute to ... Hemolysis is the abnormal breakdown of the bodys red blood cells. Health. Medicine. Fitness. Anatomy. Science. Food. ...
Hemolysis (microbiology) pronunciation, Hemolysis (microbiology) translation, English dictionary definition of Hemolysis ( ... redirected from Hemolysis (microbiology)). Also found in: Thesaurus, Medical, Encyclopedia, Wikipedia. haemolysin. ( ... Hemolysis (microbiology) - definition of Hemolysis (microbiology) by The Free Dictionary ...
During hemolysis, macrophages in the liver phagocytose damaged erythrocytes to prevent the toxic effects of cell-free ...
Hemolysis and cell-free hemoglobin drive an intrinsic mechanism for human disease. J Clin Invest. 2012;122(4):1205-1208.. View ... Hemolysis transforms liver macrophages into antiinflammatory erythrophagocytes. Marc Pfefferlé,1 Giada Ingoglia,1 Christian A. ... Haptoglobin preserves vascular nitric oxide signaling during hemolysis. Am J Respir Crit Care Med. 2016;193(10):1111-1122. ... Hemolysis and free hemoglobin revisited: exploring hemoglobin and hemin scavengers as a novel class of therapeutic proteins. ...
... (or haemolysis)-from the Latin Hemo-, Greek Αἷμα meaning blood, -lysis, meaning to break open-is the breaking open of ... Anemias caused by in vivo hemolysis are collectively called hemolytic anemias. In vitro hemolysis. In vitro hemolysis can be an ... In vivo hemolysis. Main article: Hemolytic anemia. In vivo hemolysis, which can be caused by a large number of conditions, can ... Hemolysis (or haemolysis)-from the Latin Hemo-, Greek Αἷμα meaning blood, -lysis, meaning to break open-is the breaking open of ...
Footstrike hemolysis is not only seen in long-distance runners: It has also been observed in other types of athletes, such as ... Footstrike Hemolysis: How Running Changed One Mans Blood Cells. By Cari Nierenberg 26 December 2017. ... Foot-strike hemolysis. Even though the ultramarathoner had no symptoms, to ease the mans worry, his doctors investigated what ... "footstrike hemolysis." (An ultramarathon is any race longer than marathon distance, or 26.2 miles [42.2 kilometers].) [27 ...
The hemolytic activity of milligram-sized mineral dust samples from coal mines collected in personal dust samplers were measured by a modified Harington assay method which increases the sensitivity of the technique. Measurements were conducted on a large number of samples from different mines characterized for gravimetric quartz content and the relative bulk concentration of common contaminants (A
haemolysis synonyms, haemolysis pronunciation, haemolysis translation, English dictionary definition of haemolysis. or n , pl - ... haemolysis. Also found in: Thesaurus, Medical, Encyclopedia, Wikipedia. haemolysis. , hemolysis. , haematolysis. or hematolysis ... hemolysis, haemolysis. the breaking down of erythrocytes with liberation of hemoglobin in the blood. - hemolytic, haemolytic, ... haemolysis - lysis of erythrocytes with the release of hemoglobin. haematolysis, hematolysis, hemolysis ...
If youre seeing this message, it means were having trouble loading external resources on our website.. If youre behind a web filter, please make sure that the domains * and * are unblocked. ...
... levels are indirect measures of the degree of hemolysis. An LDH level in the 1000 IU/L range (normal, , 200 IU/L) is not ... How is hemolysis assessed in the workup of thrombotic thrombocytopenic purpura (TTP)?. Updated: Feb 18, 2019 ... Lactate dehydrogenase (LDH) and bilirubin (direct and total) levels are indirect measures of the degree of hemolysis. An LDH ...
Influenza viruses cause hemolysis and fusion of cells.. Huang RT, Rott R, Klenk HD. ...
... Roustit Cécilia,1 Vallé Baptiste,1 Clouzeau ... We report a case of fatal intravascular hemolysis due to CP septicemia having a hepatic supposed starting point in the ... Nevertheless, a massive intravascular hemolysis arises only rarely in this septicemia, only from 7 to 15% of the cases. The ... Clostridium perfringens (CP) gives several clinical settings, from an asymptomatic to a massive intravascular hemolysis. ...
... Sarah A. Schubert, Behzad ... Sarah A. Schubert, Behzad Soleimani, and Walter E. Pae, "Hemolysis and Pulmonary Insufficiency following Right Ventricular ...
Boutboul D, Touzot F, Szalat R (2018) Understanding therapeutic emergencies in acute hemolysis. Intensive Care Med 44:482-485. ...
... stresses resulting from acoustically induced small-scale eddying motion near the bubble may be the mechanism of hemolysis. ...
Glucotoxicity and Oxidathre Hemolysis in G-6-PD Deficiency Message Subject (Your Name) has forwarded a page to you from ...
Genetic determinants of haemolysis in sickle cell anaemia.. Milton JN1, Rooks H, Drasar E, McCabe EL, Baldwin CT, Melista E, ...
Vienna, 31 October 2016) Worldwide, millions of people suffer from hemolysis, the breakdown of red blood cells, such as those ... Taking that into consideration, the traditional hypothesis predicted that since hemolysis leads to the release of iron- ... which suffer from hemolysis due to systemic inflammation (sepsis) or disorders such as sickle-cell disease, or malaria. In the ... uncovers the molecular mechanisms that explain how hemolysis escalates the risk for infections. This study puts to rest the old ...
Most people chose this as the best definition of haemolysis: Alternative spelling of h... See the dictionary meaning, ... Haemosiderin in the normal process of haemolysis is stored up in the cells of certain organs until required by the organism for ... In diseases where haemolysis is extreme, particularly in pernicious anaemia, there are relatively large quantities occasionally ... False An ABO incompatibility can result in red blood cell agglutination and haemolysis? ...
  • Intravascular hemolysis describes hemolysis that happens mainly inside the vasculature. (
  • Intravascular hemolysis is the state when the red blood cell ruptures as a result of the complex of complement autoantibodies attached (fixed) on the surfaces of RBCs attack and rupture RBCs' membranes, or a parasite such as Babesia exits the cell that ruptures the RBC's membrane as it goes. (
  • In the end, if the plasma concentration of the "free met-hemoglobin" and/or "free hemoglobin" is still too high for proximal tubule to absorb back into the body, then hemoglobinuria occurs, indicating an extensive intravascular hemolysis. (
  • It is important to note that although hemosiderins are also included in the urine in the setting of intravascular hemolytic hemoglobinuria, it will be detected only several days after the onset of the extensive intravascular hemolysis and will remain detectable several days after termination of intravascular hemolysis. (
  • The phenomenon tells that the detection of hemosiderin in urine is indicative of either ongoing or recent intravascular hemolysis characterized by excessive hemoglobin and/or met-hemoglobin filtered through the renal glomerulus as well as the loss of hemosiderin-laden necrotic tubular cells. (
  • It is a sporadic disease in high producing dairy cattle characterised by intravascular haemolysis , haemoglobinuria and anemia (Ok et al. (
  • These complications are consistent with NO depletion from intravascular haemolysis , and these indicate that the pathophysiologic cascade from intravascular haemolysis to NO depletion and its cardiopulmonary effects are activated in children with severe malaria. (
  • Clostridium perfringens (CP) gives several clinical settings, from an asymptomatic to a massive intravascular hemolysis. (
  • We report a case of fatal intravascular hemolysis due to CP septicemia having a hepatic supposed starting point in the emergency department. (
  • Nevertheless, a massive intravascular hemolysis arises only rarely in this septicemia, only from 7 to 15% of the cases. (
  • intravascular haemolysis , there were no adverse effects. (
  • To date, however, the influence of the mode of haemolysis (extravascular vs. intravascular) and of nonhaemolytic conditions on haptoglobin concentration and its reliability as a haemolysis marker remain poorly defined. (
  • All studied types of haemolytic disease (n = 16) were associated with markedly decreased plasma haptoglobin levels, without significant differences between intravascular vs. predominantly extravascular haemolysis. (
  • Here we characterized the pathological processes leading to acute kidney injury and inflammation during massive intravascular hemolysis, using a mouse model of phenylhydrazine (PHZ)-triggered erythrocyte destruction. (
  • We also provide detailed characterization of renal manifestations associated with acute intravascular hemolysis, which may be mediated by hemolysis-derived products upstream of heme release. (
  • This analysis provides a platform for further investigations of hemolytic diseases and associated renal injury and the evaluation of novel therapeutic strategies that target intravascular hemolysis. (
  • 8 - 10 Pulmonary hypertension, a known complication in patients with the hemolytic sub-phenotype of SCD, is thought to be caused by vasculopathy related to the chronic nitric oxide depletion from continuing intravascular hemolysis. (
  • A diagnosis of artesunate-associated hemolysis was made in this case based on the temporal relationship with therapy and the absence of other identified causes of intravascular hemolysis. (
  • Study Intravascular Hemolysis using smart web & mobile flashcards created by top students, teachers, and professors. (
  • Paroxysmal nocturnal hemoglobinuria (PNH) patients display exaggerated intravascular hemolysis and esophageal disorders. (
  • Since excess hemoglobin in the plasma causes reduced nitric oxide (NO) bioavailability and oxidative stress, we hypothesized that esophageal contraction may be impaired by intravascular hemolysis. (
  • This study aimed to analyze the alterations of the esophagus contractile mechanisms in a murine model of exaggerated intravascular hemolysis induced by phenylhydrazine (PHZ). (
  • For comparative purposes, sickle cell disease (SCD) mice were also studied, a less severe intravascular hemolysis model. (
  • In endothelial nitric oxide synthase gene-deficient mice, the contractile responses elicited by KCl and CCh were increased in the esophagus but remained unchanged with the intravascular hemolysis induced by PHZ. (
  • In conclusion, our results show that esophagus hypercontractile state occurs in association with lower NO bioavailability due to exaggerated hemolysis intravascular and oxidative stress. (
  • Moreover, our study supports the hypothesis that esophageal disorders in PNH patients are secondary to intravascular hemolysis affecting the NO-cGMP pathway. (
  • Although the severe sequelae of IV RhIG are not proven to involve intravascular hemolysis (IVH), rapid onset of hemoglobinuria is usually associated with C9-binding antibodies such as anti-A and anti-B and not the non-complement-binding anti-D. Multiple mechanisms may occur or interact and different mechanisms or explanations may account for hemoglobinemia and/or hemoglobinuria in different patients. (
  • Many species of the genus Streptococcus cause hemolysis. (
  • Gamma-hemolytic, or non-hemolytic, species do not cause hemolysis and rarely cause illness. (
  • Paxillus Involutus ingestion can cause hemolysis. (
  • Influenza viruses cause hemolysis and fusion of cells. (
  • Snake venoms and vegetable poisons such as mushrooms may also cause hemolysis. (
  • does that mean that a substance w/ a high lipid water partition coeffecient will be able to go through the cell membrane more easily and cause hemolysis faster? (
  • Sometimes if they used a needle that was too small it can also cause hemolysis. (
  • Hemolysis can lead to hemoglobinemia due to hemoglobin released into the blood plasma, which plays a significant role in the pathogenesis of sepsis and can lead to increased risk of infection due to its inhibitory effects on the innate immune system. (
  • However, during hyper-hemolytic conditions or with chronic hemolysis, haptoglobin is depleted so the remaining free hemoglobin readily distribute to tissues where it might be exposed to oxidative conditions, thus some of the ferrous heme (FeII), the oxygen-binding component of hemoglobin, of the free hemoglobin are oxidized and becoming met-hemoglobin (ferric hemoglobin). (
  • Measurements included hematocrit (Hct), free hemoglobin, and visual hemolysis index. (
  • During hemolysis, macrophages in the liver phagocytose damaged erythrocytes to prevent the toxic effects of cell-free hemoglobin and heme. (
  • Hemolysis (or haemolysis )-from the Latin Hemo- , Greek Αἷμα meaning blood, - lysis , meaning to break open-is the breaking open of red blood cells and the release of hemoglobin into the surrounding fluid ( plasma , in vivo ). (
  • Alpha hemolysis is shown by a greenish halo around the colony and is the result of hemoglobin reduction to methaemoglobin in red blood cells. (
  • After incubation for 24 h at 34 degrees C, the suspensions were centrifuged, and the percentage of hemolysis was determined by measuring the hemoglobin in the sediment and the supernatant. (
  • In drug-induced oxidative hemolysis erythrocytes undergo _______________ oxidative stress during the course of normal reactions involving oxygenation and deoxygenation of hemoglobin. (
  • In children with SCA, significant associations have been found between proteinuria and the hemolysis markers of low hemoglobin (Hb) and high lactate dehydrogenase (LDH). (
  • To evaluate biomarkers of hyperviscosity (hemoglobin levels, Hb) and hemolysis (lactate dehydrogenase, LDH levels) and correlate their role in ocular microangiopathy (conjunctival telangiectasia, non-proliferative and proliferative retinopathy). (
  • Half of these twenty analytes were affected by gross hemolysis at hemoglobin concentrations of 798 mg/dL with ordinal values of "+++++" flag level. (
  • Only three analytes (aspartate aminotransferase, direct bilirubin and lactate dehydrogenase) were affected by slight hemolysis at hemoglobin concentrations of 76 mg/dL with ordinal values of "+" flag level. (
  • Hydroxynimesulide, the main metabolite of nimesulide, prevents hydroperoxide/hemoglobin-induced hemolysis of rat erythrocytes. (
  • The suicidal death of erythrocytes should be considered a possible cause of hemolysis and plasma bilirubin overload when there is no evidence of an immune-mediated hemolytic anemia, no consumptive red blood cell disorder, no morphologic or laboratory data to suggest a problem of the red cell membrane, and no evidence of a quantitative or qualitative defect in hemoglobin synthesis. (
  • In a large study of 272 patients, 3 hemolysis was the main adverse event but decreases in hemoglobin values averaged less than 1 g/dL(10 g/L). The anemia was transient and never severe enough to require a transfusion. (
  • In the patient with the most dramatic drop in hemoglobin,the maximum decrease of 7.6 g/dL (76 g/L) occurred 8 days after treatment and she experienced ongoing hemolysis for at least 20 days, as evidenced by persistent hemoglobinuria. (
  • Differences in overall hemolysis rates and the amount of plasma samples with a concentration of free hemoglobin (fHb) above 0.5 g/L and 1 g/L were calculated. (
  • Hemolysis or haemolysis (/hiːˈmɒlɪsɪs/), also known by several other names, is the rupturing (lysis) of red blood cells (erythrocytes) and the release of their contents (cytoplasm) into surrounding fluid (e.g. blood plasma). (
  • Testing for compatible blood cells may be complicated by antibody coating of erythrocytes if there is immune-mediated hemolysis. (
  • The authors suggest that thermal injury to erythrocytes is the reason for the hemolysis. (
  • Subject: different sugars have different effects on hemolysis Title L-Sorbose but not D-tagatose induces hemolysis of dog erythrocytes in vitro. (
  • Abstract Previous investigations have demonstrated that L-sorbose induces hemolysis of dog erythrocytes. (
  • Haemolysis is the release of intracellular components from erythrocytes, thrombocytes and leukocytes into the extracellular fluid, i.e. the plasma or serum 1 . (
  • M. hyorhinis also possesses antagonistic cooperativity (reverse CAMP phenomenon) with Staphylococcus aureus beta-hemolysis, resulting in the protection of erythrocytes from the beta-hemolytic activity of S. aureus (reverse CAMP). (
  • The mechanism of the hemolysis induced by ribavirin has not been clearly determined, but oxidative stress and subsequent membrane fragility of erythrocytes have been suggested to play an important role ( 3 , 4 ). (
  • The emergency physician has to think about this complication in case of hemoglobinuria and/or signs of hemolysis associated with a septic syndrome. (
  • Patients at high risk of IVIG-associated hemolysis (defined as receipt of a 28-day cumulative dose of ≥ 2 g/kg, adjusted for ideal body weight, and non-O blood group) will be prospectively monitored using a standardized protocol for signs of hemolysis, and will be undergo additional testing for variables that have been hypothesized to increase the risk of hemolysis. (
  • Patients with blood group AB are more susceptible to a serious adverse reaction to high-dose IVIg, and would benefit from close monitoring for signs of hemolysis following treatment. (
  • Additional note by poster: The abstract fails to mention that the small amount of hemolysis induced by glucose itself was reduced by about 50% when tagatose was substituted. (
  • The amount of hemolysis, icterus and lipemia were measured using a semi-quantitative photometric test on the same instrument using the Beckman Coulter LIH reagent system. (
  • Our aim was to investigate the association of five different vacuum tubes with hemolysis rates in comparison to an aspiration system under real-life conditions and to propose an equation to estimate the amount of hemolysis, depending on the vacuum collection tube type. (
  • Lactate dehydrogenase (LDH) and bilirubin (direct and total) levels are indirect measures of the degree of hemolysis. (
  • These values were assigned by the instrument on an ordinal scale as qualitative flag levels ("N", "+", "++", "+++", "++++" and "+++++") to reflect the degree of hemolysis, icterus and lipemia in a specimen. (
  • While artemisinin-based therapies are well tolerated, recent studies have reported cases of severe delayed hemolysis after artesunate treatment for malaria ( 1 - 5 ). (
  • We report two cases of severe delayed hemolysis after the treatment with intravenous artesunate in Canada. (
  • Acute Hemolysis in the Emergency Department: Think about Clostridium perfringens! (
  • Boutboul D, Touzot F, Szalat R (2018) Understanding therapeutic emergencies in acute hemolysis. (
  • In 2000 the FDA reported the first case series involving 15 patients experiencing IV RhIG-associated acute hemolysis occurring between September 1995and March 1999, four of whom died. (
  • The incidence of acute hemolysis associated with IV RhIG is unknown. (
  • The cause of the acute hemolysis associated with IV RhIG is unknown. (
  • In vitro hemolysis can be an important unwanted effect in medical tests and can cause inaccurate results, because the contents of hemolysed red blood cells are included with the serum. (
  • In vitro hemolysis can occur in a blood sample owing prolonged storage or storage in incorrect conditions (ie too hot, too cold). (
  • Background: In vitro hemolysis, the prevailing cause of preanalytical error in routine laboratory diagnostics, might influence the reliability of several tests, affect the quality of the total testing process and jeopardize patient safety. (
  • This technique is associated with higher in vitro hemolysis rates and may even be amplified by the use of vacuum collection tubes. (
  • Thereafter, these complexes will undergo the metabolic mechanisms like extravascular hemolysis. (
  • Both had evidence of microvascular haemolysis with anaemia, increased bilirubin, increased lactate dehydrogenase and fragmented red cells on microscopy. (
  • Runners can suffer hemolytic anemia due to "footstrike hemolysis", the destruction of red blood cells in feet at foot impact. (
  • The purpose of this research study is to more accurately measure the amount of true red blood cell breakdown (hemolysis) in newborn babies with potentially problematic blood type mismatch with their mothers (ABO incompatibility), and to examine how the true level of red blood cell destruction relates to other laboratory tests obtained in newborns with jaundice. (
  • Plasma haptoglobin depletion is a reliable marker for the instant diagnosis of accelerated red cell destruction irrespective of the site of haemolysis or the presence of inflammation. (
  • The possible causes of haemolysis and consequences of cloth destruction are discussed. (
  • Superoxide anion was found to be unimportant in phenylhydrazine-induced hemolysis and destruction of oxyhemoglobin. (
  • Disruption of the balance between the lytic agent and the inhibitor may lead to the predominance of blood destruction over blood formation-in other words, to pathological hemolysis. (
  • Such hemolysis is more likely to occur when a patient's veins are difficult to find or when they collapse when blood is removed by a syringe or a modern vacuum tube. (
  • Unfortunately, increased hemolysis occurs with massive amounts of sudden blood loss, because the process of returning patient's cells must be done at a correspondingly higher speed to prevent hypotension , pH imbalance, and a number of other hemodynamic & blood level factors. (
  • The faculty for this program -William Winter, MD, and Marc Zumberg, MD-will examine how this progression of events related to the patient's anemia and hemolysis. (
  • However, high-dose IVIg can cause an adverse reaction in which the patient's red blood cells burst (hemolysis). (
  • A patient's ABO genetic make-up influences their susceptibility to IVIg-associated hemolysis. (
  • Influence of clinical factors on the haemolysis marker haptoglobin. (
  • Plasma haptoglobin determination is clinically used as parameter for haemolysis. (
  • In patients with haemolysis and a concomitant acute-phase response, however, haemolysis-dependent haptoglobin depletion was not attenuated. (
  • Interestingly, patients with a strongly positive direct antiglobulin test or high cold agglutinin titre but no further evidence for haemolysis had normal haptoglobin values. (
  • The capacity of this parameter to predict haemolysis appears to be limited in patients with liver cirrhosis and decreased haptoglobin production only. (
  • Hemolysis occurs as part of the natural life cycle of red blood cells, or it may be caused by certain conditions or drugs. (
  • Compensated hemolysis occurs when the bone marrow is able to restore balance during the early stages of red blood cell disruption, ultimately, preventing the onset of anemia. (
  • In vitro haemolysis is an interference factor if it occurs after specimen collection and changes the results of the analytical process 2 . (
  • In addition to vaso-occlusion, chronic hemolysis is also a fundamental pathological process that occurs in SCA. (
  • Pathological hemolysis occurs in hemolytic anemias and hemoglobinopathies. (
  • This may be related to antigen density - the amount of antigen on the red blood cell - which has been previously linked to the extent of hemolysis. (
  • the extent of hemolysis can therefore be judged from the amount of bilirubin in the blood and bilirubin derivatives in the feces and urine. (
  • We have investigated renal function and markers of hemolysis in a cohort of 424 adult African-British patients with sickle cell disease. (
  • This is an observational study of sickle cell patients with the aim to measure exhaled carbon monoxide levels and to correlate with genotype and standard clinical markers of hemolysis. (
  • There did not appear to be any difference in severity of hemolysis between secretors and non-secretors. (
  • hemolysis resulting from in vitro addition of phenylhydrazine to blood with red blood cells that are deficient in G6PD, with the appearance of Heinz-Ehrlich bodies. (
  • hemolysis resulting from in vitro addition of phenylhydrazine to blood with red blood cells that are deficient in glucose-6-phosphate dehydrogenase (G6PD), with the appearance of Heinz-Ehrlich bodies. (
  • Our findings suggest that hemolysis associated with ribavirin is triggered by an energy crisis and consequent oxidative stress, thus having implications for the prevention of such hemolysis. (
  • Because in vivo hemolysis destroys red blood cells, in uncontrolled, chronic or severe cases it can lead to hemolytic anemia. (
  • Extrinsic hemolysis is caused by the red blood cell's environment: Immune-mediated causes could include transient factors as in Mycoplasma pneumoniae infection (cold agglutinin disease) or permanent factors as in autoimmune diseases like autoimmune hemolytic anemia (itself more common in diseases such as systemic lupus erythematosus, rheumatoid arthritis, Hodgkin's lymphoma, and chronic lymphocytic leukemia). (
  • Treatment for hemolysis, and the subsequent development of hemolytic anemia, is dependent on the type and reason for the anemia. (
  • Individuals experiencing hemolysis-induced anemia may develop a variety of symptoms. (
  • There are a variety of diagnostic tests used to confirm the presence of hemolysis and hemolytic anemia. (
  • In vivo hemolysis, which can be caused by a large number of conditions, can lead to anemia . (
  • Exclusion criteria include the presence of an alternate cause of anemia, including blood loss, other drug-induced hemolysis, anemia associated with chemotherapy for cancer, or hemolysis associated with an underlying disease or participation in another ongoing study. (
  • Some patients who develop hemolysis may require transfusion of red blood cells to treat the resulting anemia. (
  • The goal of the study is to define the incidence and dynamics of IVIG-mediated hemolysis and identify patient and product-related factors that may predict which patients are especially at risk. (
  • The incidence of hemolysis was highest for patients with AB blood who had the A1 subtype (group A1B) . (
  • The incidence rate for acute nonimmune-mediated haemolysis is rare. (
  • After transfusion of red blood cells (RBCs) via syringe in the operating room, each patient experienced discolored urine, laboratory evidence of hemolysis , and acute kidney injury. (
  • Constant manual pressure (1.43 ± 0.49 mL/sec) or syringe pump (2 mL/min) was used and a subset was timed.The valve increased hemolysis during manual transfusion using both catheters with washed and unwashed RBCs. (
  • Mechanical hemolysis is due to excessive mechanical forces that damage red blood cells (RBCs). (
  • Beta hemolysis is shown by a clear halo around the colony and is produced by complete hemolysis of the red blood cells. (
  • In Clostridium perfringens ( 12 ) and Corynebacterium pseudotuberculosis ( 13 , 14 ), however, the rare antagonistic interaction (reverse CAMP phenomenon) was described where the beta-hemolysis of staphylococci was inhibited, apparently through the activity of a phospholipase D (PLD) ( 14 ). (
  • What does 'beta-hemolysis on cna blood agar plate' mean? (
  • If the tests are on patients with the haemolytic syndrome (in-vivo haemolysis), then differentiating it from in vitro haemolysis, mostly resulting from inappropriate specimen collection, is rarely possible. (
  • When examining the reason, in only 3.2 % of haemolysed samples was in vivo haemolysis the cause. (
  • In-vivo haemolysis is caused by antibodies, biochemically through medications, by toxic substances, through hereditary factors (e.g. haemoglobinopathies), through enzyme defects (acholuric jaundice) or by infections (e.g. malaria). (
  • When suspecting in vivo haemolysis the plasma should be checked to exclude the possibility of additional in vitro haemolysis1 caused by the coagulation process. (
  • When the DAT is performed without an indication for in vivo haemolysis, there is a high risk of false-positive results. (
  • Severe hemolysis and transfusion reactions after treatment with BGB-3111 and PD-1 antibody for Waldenström macroglobulinemia 29439186 2019 03 06 1592-8721 103 5 2018 05 Haematologica Haematologica Severe hemolysis and transfusion reactions after treatment with BGB-3111 and PD-1 antibody for Waldenström macroglobulinemia. (
  • The first case of 3-year-old boy died from severe hemolysis which was caused by the regurgitant jet from the truncal valve running against the rough-surfaced Dacron patch for the VSD. (
  • If my blood results say 'slight hemolysis, ' what does that mean? (
  • Two different blood tests listed slight hemolysis. (
  • On multiple blood tests, there is a note of slight hemolysis. (
  • antibiotics to treat underlying infection, inotropic support for patients with cardiac decompensation, steroids for immune haemolysis , and chemotherapy for leukaemia / lymphoma. (
  • Fatal immune haemolysis associated with nomifensine. (
  • Microangiopathic haemolytic anaemia (MAHA) is a non-immune haemolysis with prominent red cell fragmentation observed on peripheral blood film. (
  • Rule out immune haemolysis by performing a direct antiglobulin test (DAT) and repeat patient ABO group. (
  • In the case, which was published Dec. 13 in the journal BMJ Case Reports , a 41-year-old man who regularly trained for and competed in 50- to 100-mile ultramarathons was diagnosed with a condition called "footstrike hemolysis. (
  • Footstrike hemolysis is not only seen in long-distance runners: It has also been observed in other types of athletes, such as cyclists and swimmers, and in nonathletes, such as soldiers after a strenuous march, DeGeorge said. (
  • Hemolysis is the breakdown of red blood cells. (
  • The sugar-water hemolysis test is a blood test to detect fragile red blood cells. (
  • Because the feeding process of the Plasmodium parasites damages red blood cells, malaria is sometimes called "parasitic hemolysis" in medical literature. (
  • Better platelet function, less fibrinolysis and less hemolysis in re-transfused residual pump blood with the Ringer's chase technique - a randomized pilot study. (
  • Platelet activation and function (impedance aggregometry), coagulation and hemolysis variables were assessed in the re- transfused blood and in the patients before, during and after surgery. (
  • Hemolysis risk after packed red blood cells transfusion with infusion pumps To evaluate the hemolysis biomarkers of packed red blood cells transfused by two different linear peristaltic infusion pumps at two infusion rates.An experimental and randomized study was designed simulating the clinical practice of transfusion . (
  • The abnormal breakdown of the body's red blood cells is known as hemolysis. (
  • Extensive blood testing can determine whether a person has hemolysis. (
  • Streptococci are differentiated by hemolysis of red blood cells on blood agar (BA) plates. (
  • Gamma hemolysis is shown as no hemolysis or discoloration of the blood. (
  • Vienna, 31 October 2016) Worldwide, millions of people suffer from hemolysis, the breakdown of red blood cells, such as those afflicted with sickle-cell disease, malaria or sepsis. (
  • For decades, iron has been considered the prime suspect responsible for the high rate of bacterial infections in patients with hemolysis (bursting of red blood cells). (
  • Red blood cells with (right) and without (left and middle) hemolysis. (
  • To the editor: Doctor Berkes and colleagues ( Ann Intern Med 83:363-364, 1975) report the delayed onset of protracted hemolysis after the extracorporeal overheating of blood to 50 °C during hemodialysis. (
  • It contains pooled antibodies from thousands of donors, and a minor population of these antibodies are thought to be involved in triggering red blood cell hemolysis. (
  • people with blood group A, B, or AB are much more likely to have IVIg-associated hemolysis. (
  • This supports the idea that red blood cell antigens may play a role in susceptibility to IVIg-associated hemolysis. (
  • The researchers determined three plausible genetic risk factors that could make patients more susceptible to IVIg-associated hemolysis, then did genetic testing on 42 patients with blood groups A, B or AB who had received high-dose IVIg. (
  • Soluble A and B antigens have been found to protect against red blood cell hemolysis, but not everyone has these. (
  • The researchers examined the number of copies of the Fcγ receptor gene each patient had, and looked for certain mutations within this receptor that might influence susceptibility to red blood cell hemolysis. (
  • Having the O allele or the A2 subtype of group A blood seemed to protect against hemolysis. (
  • Visual pre-analytical inspection of centrifuged blood samples for haemolysis is the reason for rejection in 60% of the rejected samples. (
  • We investigated the prevalence of detectable haemolysis in all routine venous blood samples to identify differences in preanalytical quality. (
  • Defensin concentrations that inhibited hemolysis by ALO and listeriolysin did not prevent these toxins from binding either to red blood cells or to cholesterol. (
  • Hemolysis Agent For Blood Cell Analyzer Industry-South America Market Status and Trend Report 2013-2023 offers a comprehensive analysis on Hemolysis Agent For Blood Cell Analyzer Industry industry, standing on the readers' perspective, delivering detailed market data and penetrating insights. (
  • An assay was developed in which the degree of in vitro spleen cell-mediated sheep red blood cell (SRBC) hemolysis was quantitated spectrophotometrically (QHS). (
  • test results as follow - ph urine 6.0 - glucose negative - bilirubin neg - protein neg- everything negative except says blood, urine - value is trace / hemolysis standard range negative mean? (
  • We retrospectively evaluated hemolysis data of plasma samples from our ED, where blood is drawn through IV catheters. (
  • The hemolysis rate of one partial-draw blood collection tube was comparable to those of the aspiration system. (
  • If IV catheters are used for blood collection, hemolysis rates directly correlate with the vacuum within the tubes and can be estimated by the proposed formula. (
  • By the use of partial-draw vacuum blood collection tubes, hemolysis rates in IV catheter collections can be reduced to levels comparable with collections performed by aspiration systems. (
  • All three patients suffered an episode of massive acute haemolysis, in the absence of blood group incompatibilities, infection, or ingestion of oxidising agents known to trigger haemolysis. (
  • We describe three neonates with G6PD deficiency who developed severe hyperbilirubinaemia requiring exchange transfusion because of massive acute haemolysis, in the absence of any obvious blood group incompatibilities, infection, or ingestion of oxidising agents known to trigger haemolysis by either the mother or infant. (
  • METHODS: The hemolysis index (HI) of 43,875 samples was measured before (n=21,512) and after (n=22,363) the switch of blood collection responsibilities. (
  • Drug-induced hemolysis can occur either by an immune-mediated mechanism or a non immune-mediated mechanism. (
  • In patients with ongoing hemolysis who have received multiple transfusions, the drug-induced component of hemolysis may be less of a contributing factor and a full transfusion reaction work-up should be considered. (
  • The loss of cytoskeletal resilience results in life-threatening conditions for millions of people worldwide, which suffer from hemolysis due to systemic inflammation (sepsis) or disorders such as sickle-cell disease, or malaria. (
  • A frequent adverse effect related to massive hemolysis is the renal injury and inflammation. (
  • Hemolysis transforms liver macrophages into antiinflammatory erythrophagocytes. (
  • In diseases where haemolysis is extreme, particularly in pernicious anaemia, there are relatively large quantities occasionally as much as ten times the normal amount of haemosiderin deposited in the liver. (
  • Factors relevant to mode of preterm delivery with syndrome of HELLP (hemolysis, elevated liver enzymes, and low platelets). (
  • 34 weeks) gestation complicated by the syndrome of hemolysis, elevated liver enzymes, and low platelets (HELLP). (
  • Hemolysis, elevated liver enzymes, and low platelet count (HELLP) syndrome is a severe condition of pregnancy that is associated with significant morbidity and mortality. (
  • The causes of hemolysis, elevated liver enzymes, and low platelet count (HELLP) syndrome or hepatic hemorrhage as a serious complication of HELLP are not known. (
  • Although laboratory instrumentation is now routinely equipped with systems capable of automatically testing and eventually correcting for hemolysis interference, to our knowledge there are no reports that have compared the efficiency of different analytical platforms for identifying and classifying specimens with hemolysis. (
  • Haemolysis is an important interference factor that must be considered when making laboratory measurements. (
  • Interference from hemolysis was detected for 20 of 40 tested analytes. (
  • Mechanical hemolysis in pediatric patients associated with rapid transfusion and one-way valve. (
  • This study puts to rest the old belief that iron availability favors bacterial infections in hemolytic disorders, and moreover reveals a new treatment approach to protect patients with hemolysis from infections. (
  • Taking that into consideration, the traditional hypothesis predicted that since hemolysis leads to the release of iron-containing heme, the threat of serious bacterial infections in these patients was attributed to the excess availability of circulating iron (heme). (
  • However, other factors are at play here, as not all A, B or AB patients develop hemolysis. (
  • Of these 6 patients, all had IVIg-associated hemolysis. (
  • Subtypes of group A ( A1 or A2 ) seemed to influence risk: Of the 15 patients with an A gene who had hemolysis. (
  • In all previous case reports of delayed hemolysis, patients received World Health Organization-prequalified, but not GMP-certified, artesunate ( 1 - 5 ). (
  • Results from a study recently published in the British Journal of Haematology indicate a pathological cycle with hemolysis, inflammatory response, and iron overload (IO) in patients with congenital hemolytic anemias (CHAs) . (
  • Overall, these findings suggest the existence of a vicious cycle between chronic hemolysis, inflammatory response, and IO in patients with CHAs," concluded the authors. (
  • Delayed hemolysis after parenteral artesunate has been described in Europe and Asia, but until recently had not been reported in patients receiving the artesunate product used in the United States and Canada. (
  • Supportive therapy was continued, and hemolysis ceased spontaneously 10 days after onset. (
  • lepturus causes severe and fatal haemolysis , secondary renal failure, deep and necrotic ulcers, ankylosis of the joints, psychological problems, and death. (
  • Studies have questioned whether renal dysfunction in sickle cell disease is linked to hemolysis-associated vasculopathy. (
  • Most non-immune mediated haemolysis situations are benign, but life-threateneing haemolysis with severe anaemia and renal failure may occur. (
  • Even if haemolysis is not visually detectable, a discharge of intracellular constituents into the plasma/serum can have occurred. (
  • The pathophysiology of IVIG-associated hemolysis will be characterized by tracking changes in serum complement levels, performing extended cytokine profiling, and conducting mononuclear phagocyte activity assays using patient monocytes. (
  • 2] SCD is a hemoglobinopathy clinically characterized by chronic haemolysis , susceptibility to frequent infections, growth retardation, spleenic sequestration and intermittent episodes of painful vasoocclusive crisis affecting different organs. (
  • Its measurement by means of the haemolysis index, that also has become available on coagulation analyzers (15), was proven to be reliable and practical (16), so that this practice should be encouraged to provide an objective and virtually incontestable measure of sample quality. (
  • Hydrodynamic stresses resulting from acoustically induced small-scale eddying motion near the bubble may be the mechanism of hemolysis. (
  • Several medications are known to cause non immune-mediated hemolysis through an oxidative mechanism, usually in the setting of some forms of glucose-6-phosphate dehydrogenase (G6PD) deficiency. (
  • It was anticipated that this approach would help to clarify the mechanism responsible for ribavirin-induced hemolysis. (
  • Genetic determinants of haemolysis in sickle cell anaemia. (
  • Perhaps by independently down-regulating expression of the HBA1/HBA2 genes, variants of the HBA1/HBA2 gene regulatory loci, tagged by rs7203560, reduce haemolysis in sickle cell anaemia. (
  • In this article we present two cases of haemolytic anaemia in which the DAT gives further indication of the cause of haemolysis. (
  • With a concentration of 0.4 per cent NaCl a decrease in hemolysis takes place concomitant with an increase in concentrations of sodium lactate. (
  • With still lower concentrations of NaCl, hemolysis was intensified to such an extent that with 0.3 per cent NaCl hemolysis was complete notwithstanding the addition of 1:1,000 lactic acid and that with 0.2 per cent NaCl it was complete with 1:500 lactic acid, lactic acid being used in the form of sodium lactate solution. (
  • Hereditary conditions, such as sickle cell disease and G6PD deficiency disorder, may also contribute to the development of hemolysis. (
  • The principal study objective is to assess if exhaled carbon monoxide levels are a non-invasive marker of hemolysis in subjects with sickle cell disease (SCD). (
  • We report a case of severe delayed hemolysis after administration of GMP artesunate to treat a patient with severe malaria. (
  • Soluble antigens in plasma allow mismatched transfusion without hemolysis. (
  • Haemolysis is visible as a red colouration of the plasma or serum after centrifugation of the sample. (
  • Methods: Haemolysis index (HI) values were obtained from a Vitros 5,1 in the routine clinical chemistry laboratory for samples collected in primary health care centres (PHCs), nursing homes, and a hospital emergency department (ED). Haemolysis was defined as a HI ≥15 (detection limit). (
  • Reactive oxygen radicals generated during infections such as trypanosomosis can attack erythrocyte membrane, induce its oxidation and thus trigger haemolysis [23]. (
  • In the latest edition of Nature Immunology, Sylvia Knapp's Group at the CeMM Research Center for Molecular Medicine of the Austrian Academy of Sciences and the Medical University of Vienna, uncovers the molecular mechanisms that explain how hemolysis escalates the risk for infections. (
  • Complications associated with hemolysis and hemolytic anemias may include infection, kidney failure, and cardiovascular failure. (
  • Stopping the offending drug early is important to arrest hemolysis and prevent further complications. (
  • Hemolysis may occur in vivo or in vitro (inside or outside the body). (
  • Hemolysis can also occur at the time of venipuncture , but it is uncommon when the venipuncture is straightforward and the phlebotomist is experienced. (
  • Hemolysis may occur if the centrifuge rotates too quickly (generally greater than 500 rpm)-essentially this is hemolysis occurring outside of the body. (
  • Massive acute haemolysis is thought not to occur in the absence of trigger factors 2, 6 in the newborn period. (
  • Individuals with a deficiency of G6PD cannot reduce oxidized glutathione to reduced glutathione, leading to oxidative damage and eventual hemolysis. (
  • The primary safety data collected in this study will help to understand the hemolysis risk to both G6PD-normal and G6PD-deficient subjects. (
  • Hyperbilirubinaemia in neonates with G6PD deficiency is thought to be secondary to reduced hepatic conjugation and excretion of bilirubin 1, 2, 4, 5 rather than increased bilirubin production resulting from haemolysis. (
  • G6PD screening revealed deficiency, taken at the time of maximal haemolysis and after six weeks follow up (Hb was then 124 g/l). (