DiseasesChemicals and DrugsPhenomena and Processes
Hemolysin ProteinsHemolysisHemolysin Factors
Hemolysin Proteins
Proteins from BACTERIA and FUNGI that are soluble enough to be secreted to target ERYTHROCYTES and insert into the membrane to form beta-barrel pores. Biosynthesis may be regulated by HEMOLYSIN FACTORS.
Hemolysis
The destruction of ERYTHROCYTES by many different causal agents such as antibodies, bacteria, chemicals, temperature, and changes in tonicity.
Hemolysin Factors
Plasmids controlling the synthesis of hemolysin by bacteria.

Alpha-toxin and gamma-toxin jointly promote Staphylococcus aureus virulence in murine septic arthritis. (1/3113)

Septic arthritis is a common and feared complication of staphylococcal infections. Staphylococcus aureus produces a number of potential virulence factors including certain adhesins and enterotoxins. In this study we have assessed the roles of cytolytic toxins in the development of septic arthritis by inoculating mice with S. aureus wild-type strain 8325-4 or isogenic mutants differing in the expression of alpha-, beta-, and gamma-toxin production patterns. Mice inoculated with either an alpha- or beta-toxin mutant showed degrees of inflammation, joint damage, and weight decrease similar to wild-type-inoculated mice. In contrast, mice inoculated with either double (alpha- and gamma-toxin-deficient)- or triple (alpha-, beta-, and gamma-toxin-deficient)-mutant S. aureus strains showed lower frequency and severity of arthritis, measured both clinically and histologically, than mice inoculated with the wild-type strain. We conclude that simultaneous production of alpha- and gamma-toxin is a virulence factor in S. aureus arthritis.  (+info)

Evolutionary relationships of pathogenic clones of Vibrio cholerae by sequence analysis of four housekeeping genes. (2/3113)

Studies of the Vibrio cholerae population, using molecular typing techniques, have shown the existence of several pathogenic clones, mainly sixth-pandemic, seventh-pandemic, and U.S. Gulf Coast clones. However, the relationship of the pathogenic clones to environmental V. cholerae isolates remains unclear. A previous study to determine the phylogeny of V. cholerae by sequencing the asd (aspartate semialdehyde dehydrogenase) gene of V. cholerae showed that the sixth-pandemic, seventh-pandemic, and U.S. Gulf Coast clones had very different asd sequences which fell into separate lineages in the V. cholerae population. As gene trees drawn from a single gene may not reflect the true topology of the population, we sequenced the mdh (malate dehydrogenase) and hlyA (hemolysin A) genes from representatives of environmental and clinical isolates of V. cholerae and found that the mdh and hlyA sequences from the three pathogenic clones were identical, except for the previously reported 11-bp deletion in hlyA in the sixth-pandemic clone. Identical sequences were obtained, despite average nucleotide differences in the mdh and hlyA genes of 1.52 and 3.25%, respectively, among all the isolates, suggesting that the three pathogenic clones are closely related. To extend these observations, segments of the recA and dnaE genes were sequenced from a selection of the pathogenic isolates, where the sequences were either identical or substantially different between the clones. The results show that the three pathogenic clones are very closely related and that there has been a high level of recombination in their evolution.  (+info)

Role of Listeria monocytogenes exotoxins listeriolysin and phosphatidylinositol-specific phospholipase C in activation of human neutrophils. (3/3113)

Polymorphonuclear leukocytes (PMN) are essential for resolution of infections with Listeria monocytogenes. The present study investigated the role of the listerial exotoxins listeriolysin (LLO) and phosphatidylinositol-specific phospholipase C (PlcA) in human neutrophil activation. Different Listeria strains, mutated in individual virulence genes, as well as purified LLO were used. Coincubation of human neutrophils with wild-type L. monocytogenes provoked PMN activation, occurring independently of phagocytosis events, with concomitant elastase secretion, leukotriene generation, platelet-activating factor (PAF) synthesis, respiratory burst, and enhanced phosphoinositide hydrolysis. Degranulation and leukotriene formation were noted to be solely dependent on LLO expression, as these features were absent when the LLO-defective mutant EGD- and the avirulent strain L. innocua were used. These effects were fully reproduced by a recombinant L. innocua strain expressing LLO (INN+) and by the purified LLO molecule. LLO secretion was also required for PAF synthesis. However, wild-type L. monocytogenes was more potent in eliciting PAF formation than mutants expressing LLO, suggesting the involvement of additional virulence factors. This was even more obvious for phosphoinositide hydrolysis and respiratory burst: these events were provoked not only by INN+ but also by the LLO-defective mutant EGD- and by a recombinant L. innocua strain producing listerial PlcA. We conclude that human neutrophils react to extracellularly provided listerial exotoxins by rapid cell activation. Listeriolysin is centrally involved in triggering degranulation and lipid mediator generation, and further virulence factors such as PlcA apparently contribute to trigger neutrophil phosphoinositide hydrolysis and respiratory burst. In this way, listerial exotoxins may influence the host defense against infections with L. monocytogenes.  (+info)

Vibrio parahaemolyticus thermostable direct hemolysin modulates cytoskeletal organization and calcium homeostasis in intestinal cultured cells. (4/3113)

Vibrio parahaemolyticus is a marine bacterium known to be the leading cause of seafood gastroenteritis worldwide. A 46-kDa homodimer protein secreted by this microorganism, the thermostable direct hemolysin (TDH), is considered a major virulence factor involved in bacterial pathogenesis since a high percentage of strains of clinical origin are positive for TDH production. TDH is a pore-forming toxin, and its most extensively studied effect is the ability to cause hemolysis of erythrocytes from different mammalian species. Moreover, TDH induces in a variety of cells cytotoxic effects consisting mainly of cell degeneration which often leads to loss of viability. In this work, we examined the cellular changes induced by TDH in monolayers of IEC-6 cells (derived from the rat crypt small intestine), which represent a useful cell model for studying toxins from enteric bacteria. In experimental conditions allowing cell survival, TDH induces a rapid transient increase in intracellular calcium as well as a significant though reversible decreased rate of progression through the cell cycle. The morphological changes seem to be dependent on the organization of the microtubular network, which appears to be the preferential cytoskeletal element involved in the cellular response to the toxin.  (+info)

Hyperproduction of alpha-hemolysin in a sigB mutant is associated with elevated SarA expression in Staphylococcus aureus. (5/3113)

To evaluate the role of SigB in modulating the expression of virulence determinants in Staphylococcus aureus, we constructed a sigB mutant of RN6390, a prototypic S. aureus strain. The mutation in the sigB gene was confirmed by the absence of the SigB protein in the mutant on an immunoblot as well as the failure of the mutant to activate sigmaB-dependent promoters (e.g., the sarC promoter) of S. aureus. Phenotypic analysis indicated that both alpha-hemolysin level and fibrinogen-binding capacity were up-regulated in the mutant strain compared with the parental strain. The increase in fibrinogen-binding capacity correlated with enhanced expression of clumping factor and coagulase on immunoblots. The effect of the sigB mutation on the enhanced expression of the alpha-hemolysin gene (hla) was primarily transcriptional. Upon complementation with a plasmid containing the sigB gene, hla expression returned to near parental levels in the mutant. Detailed immunoblot analysis as well as a competitive enzyme-linked immunosorbent assay of the cell extract of the sigB mutant with anti-SarA monoclonal antibody 1D1 revealed that the expression of SarA was higher in the mutant than in the parental control. Despite an elevated SarA level, the transcription of RNAII and RNAIII of the agr locus remained unaltered in the sigB mutant. Because of a lack of perturbation in agr, we hypothesize that inactivation of sigB leads to increased expression of SarA which, in turn, modulates target genes via an agr-independent but SarA-dependent pathway.  (+info)

Probing the function of Bordetella bronchiseptica adenylate cyclase toxin by manipulating host immunity. (6/3113)

We have examined the role of adenylate cyclase-hemolysin (CyaA) by constructing an in-frame deletion in the Bordetella bronchiseptica cyaA structural gene and comparing wild-type and cyaA deletion strains in natural host infection models. Both the wild-type strain RB50 and its adenylate cyclase toxin deletion (DeltacyaA) derivative efficiently establish persistent infections in rabbits, rats, and mice following low-dose inoculation. In contrast, an inoculation protocol that seeds the lower respiratory tract revealed significant differences in bacterial numbers and in polymorphonuclear neutrophil recruitment in the lungs from days 5 to 12 postinoculation. We next explored the effects of disarming specific aspects of the immune system on the relative phenotypes of wild-type and DeltacyaA bacteria. SCID, SCID-beige, or RAG-1(-/-) mice succumbed to lethal systemic infection following high- or low-dose intranasal inoculation with the wild-type strain but not the DeltacyaA mutant. Mice rendered neutropenic by treatment with cyclophosphamide or by knockout mutation in the granulocyte colony-stimulating factor locus were highly susceptible to lethal infection by either wild-type or DeltacyaA strains. These results reveal the significant role played by neutrophils early in B. bronchiseptica infection and by acquired immunity at later time points and suggest that phagocytic cells are a primary in vivo target of the Bordetella adenylate cyclase toxin.  (+info)

Resistance of paroxysmal nocturnal hemoglobinuria cells to the glycosylphosphatidylinositol-binding toxin aerolysin. (7/3113)

Paroxysmal nocturnal hemoglobinuria (PNH) is a clonal stem cell disorder caused by a somatic mutation of the PIGA gene. The product of this gene is required for the biosynthesis of glycosylphosphatidylinositol (GPI) anchors; therefore, the phenotypic hallmark of PNH cells is an absence or marked deficiency of all GPI-anchored proteins. Aerolysin is a toxin secreted by the bacterial pathogen Aeromonas hydrophila and is capable of killing target cells by forming channels in their membranes after binding to GPI-anchored receptors. We found that PNH blood cells (erythrocytes, lymphocytes, and granulocytes), but not blood cells from normals or other hematologic disorders, are resistant to the cytotoxic effects of aerolysin. The percentage of lysis of PNH cells after aerolysin exposure paralleled the percentage of CD59(+) cells in the samples measured by flow cytometry. The kinetics of red blood cell lysis correlated with the type of PNH erythrocytes. PNH type III cells were completely resistant to aerolysin, whereas PNH type II cells displayed intermediate sensitivity. Importantly, the use of aerolysin allowed us to detect PNH populations that could not be detected by standard flow cytometry. Resistance of PNH cells to aerolysin allows for a simple, inexpensive assay for PNH that is sensitive and specific. Aerolysin should also be useful in studying PNH biology.  (+info)

Localization and environment of tryptophans in soluble and membrane-bound states of a pore-forming toxin from Staphylococcus aureus. (8/3113)

The location and environment of tryptophans in the soluble and membrane-bound forms of Staphylococcus aureus alpha-toxin were monitored using intrinsic tryptophan fluorescence. Fluorescence quenching of the toxin monomer in solution indicated varying degrees of tryptophan burial within the protein interior. N-Bromosuccinimide readily abolished 80% of the fluorescence in solution. The residual fluorescence of the modified toxin showed a blue-shifted emission maximum, a longer fluorescence lifetime as compared to the unmodified and membrane-bound alpha-toxin, and a 5- to 6-nm red edge excitation shift, all indicating a restricted tryptophan environment and deeply buried tryptophans. In the membrane-bound form, the fluorescence of alpha-toxin was quenched by iodide, indicating a conformational change leading to exposure of some tryptophans. A shorter average lifetime of tryptophans in the membrane-bound alpha-toxin as compared to the native toxin supported the conclusions based on iodide quenching of the membrane-bound toxin. Fluorescence quenching of membrane-bound alpha-toxin using brominated and spin-labeled fatty acids showed no quenching of fluorescence using brominated lipids. However, significant quenching was observed using 5- and 12-doxyl stearic acids. An average depth calculation using the parallax method indicated that the doxyl-quenchable tryptophans are located at an average depth of 10 A from the center of the bilayer close to the membrane interface. This was found to be in striking agreement with the recently described structure of the membrane-bound form of alpha-toxin.  (+info)

The pore-forming toxin listeriolysin O (LLO), an important virulence factor thats - casein kinases mediate the...The pore-forming toxin listeriolysin O (LLO), an important virulence factor thats - casein kinases mediate the...
The pore-forming toxin listeriolysin O (LLO), an important virulence factor thats secreted by (infection. attacks all around the globe, and specifically the antibiotic-resistant strains which have been isolated from human beings and the surroundings, this bacterium can be a significant concern for open public health (2C4). can be an invasive bacterium, and it expresses many virulence elements that are extremely connected with cell invasion, intracellular bacterial success, and cell-to-cell growing. Pursuing their internalization into focus on cells, including both phagocytic cells and different non-phagocytic cells, bacterias either are wiped out or turn out escaping from the principal internalization vesicle in to the cytoplasm (5). Once inside the cytosol, the bacterias grow rapidly, plus they utilize the web host actin cytoskeleton by expressing a surface area protein known as ActA to create F-actin, which gives for bacterial motility buy 38226-84-5 and dissemination into neighboring cells. ...
http://casein-kinases.com/the-pore-forming-toxin-listeriolysin-o-llo-an-important-virulence-factor-thats/
OPUS Würzburg | The Deletion of Several Amino Acid Stretches of Escherichia coli Alpha-Hemolysin (HlyA) Suggests That the...OPUS Würzburg | The Deletion of Several Amino Acid Stretches of Escherichia coli Alpha-Hemolysin (HlyA) Suggests That the...
Escherichia coli α-hemolysin (HlyA) is a pore-forming protein of 110 kDa belonging to the family of RTX toxins. A hydrophobic region between the amino acid residues 238 and 410 in the N-terminal half of HlyA has previously been suggested to form hydrophobic and/or amphipathic α-helices and has been shown to be important for hemolytic activity and pore formation in biological and artificial membranes. The structure of the HlyA transmembrane channel is, however, largely unknown. For further investigation of the channel structure, we deleted in HlyA different stretches of amino acids that could form amphipathic β-strands according to secondary structure predictions (residues 71-110, 158-167, 180-203, and 264-286). These deletions resulted in HlyA mutants with strongly reduced hemolytic activity. Lipid bilayer measurements demonstrated that HlyAΔ71-110 and HlyAΔ264-286 formed channels with much smaller single-channel conductance than wildtype HlyA, whereas their channel-forming activity was virtually
https://opus.bibliothek.uni-wuerzburg.de/frontdoor/index/index/searchtype/authorsearch/author/%22Ludwig%2C+Albrecht%22/start/0/rows/10/subjectfq/red+blood+cells/docId/11811
Influence of Cys-130 S. aureus Alpha-toxin on Planar Lipid Bilayer and Erythrocyte Membranes, The Journal of Membrane Biology |...Influence of Cys-130 S. aureus Alpha-toxin on Planar Lipid Bilayer and Erythrocyte Membranes, The Journal of Membrane Biology |...
Read Influence of Cys-130 S. aureus Alpha-toxin on Planar Lipid Bilayer and Erythrocyte Membranes, The Journal of Membrane Biology on DeepDyve, the largest online rental service for scholarly research with thousands of academic publications available at your fingertips.
https://www.deepdyve.com/lp/springer_journal/influence-of-cys-130-s-aureus-alpha-toxin-on-planar-lipid-bilayer-and-piS9jf0hLJ
Are amyloid diseases caused by protein aggregates that mimic bacterial pore-forming toxins?<...
TY - JOUR. T1 - Are amyloid diseases caused by protein aggregates that mimic bacterial pore-forming toxins?. AU - Lashuel, Hilal A.. AU - Lansbury, Peter T.. PY - 2006/5/1. Y1 - 2006/5/1. N2 - Protein fibrillization is implicated in the pathogenesis of most, if not all, age-associated neurodegenerative diseases, but the mechanism(s) by which it triggers neuronal death is unknown. Reductionist in vitro studies suggest that the amyloid protofibril may be the toxic species and that it may amplify itself by inhibiting proteasome-dependent protein degradation. Although its pathogenic target has not been identified, the properties of the protofibril suggest that neurons could be killed by unregulated membrane permeabilization, possibly by a type of protofibril referred to here as the amyloid pore. The purpose of this review is to summarize the existing supportive circumstantial evidence and to stimulate further studies designed to test the validity of this hypothesis.. AB - Protein fibrillization is ...
https://qfrd.pure.elsevier.com/en/publications/are-amyloid-diseases-caused-by-protein-aggregates-that-mimic-bact
OPUS Würzburg | SearchOPUS Würzburg | Search
We have cloned the chromosomal hemolysin determinants from Escherichia coli strains belonging to the four O-serotypes 04, 06, 018, and 075, The hemolysin-producing clones were isolated from gene banks of these strains which were constructed by inserting partial Sau3A fragments of chromosomal DNA into the cosmid pJC74. The hemolytic cosmid clones were relatively stable. The inserts were further sub cloned either as Sail fragments in pACYC184 or as BamHI-SaLI fragments in a recombinant plasmid (pANN202) containing cistron C (hlye) of the plasmid-encoded hemolysin determinant. Detailed restriction maps of each of these determinants were constructed, and it was found that, despite sharing overall homology, the determinants exhibited minor specific differences in their structure, These appeared to be restricted to cistron A (hlyA), which is the structural gene for hemolysin. In the gene banks of two of these hemolytic strains, we could also identify clones which carried the genetic determinants for ...
https://opus.bibliothek.uni-wuerzburg.de/solrsearch/index/search/searchtype/all/rows/10/languagefq/eng/facetNumber_subject/all/yearfq/1982/start/0/author_facetfq/Hughes%2C+Colin
Maturation of hemolysin-producing cell clones. I. The kinetics of the by G C. SaundersMaturation of hemolysin-producing cell clones. I. The kinetics of the by G C. Saunders
Saunders, G C., Maturation of hemolysin-producing cell clones. I. The kinetics of the induction period of an in vitro hemolysin response to erythrocyte antigen. (1969). Subject Strain Bibliography 1969. 634 ...
https://mouseion.jax.org/ssbb1969/634/
Mitogen-activated protein kinase pathways defend against bacterial pore-forming toxinsMitogen-activated protein kinase pathways defend against bacterial pore-forming toxins
Cytolytic pore-forming toxins are important for the virulence of many disease-causing bacteria. How target cells molecularly respond to these toxins and whether or not they can mount a defense are poorly understood. By using microarrays, we demonstrate that the nematode Caenorhabditis elegans responds robustly to Cry5B, a member of the pore-forming Crystal toxin family made by Bacillus thuringiensis. This genomic response is distinct from that seen with a different stressor, the heavy metal cadmium. A p38 mitogen-activated protein kinase (MAPK) kinase and a c-Jun N-terminal-like MAPK are both transcriptionally up-regulated by Cry5B. Moreover, both MAPK pathways are functionally important because elimination of either leads to animals that are (i) hypersensitive to a low, chronic dose of toxin and (ii) hypersensitive to a high, brief dose of toxin such that the animal might naturally encounter in the wild. These results extend to mammalian cells because inhibition of p38 results in the hypersensitivity
https://infoscience.epfl.ch/record/133518
Structure of staphylococcal alpha-hemolysin, a heptameric transmembrane pore. - PubMed - NCBIStructure of staphylococcal alpha-hemolysin, a heptameric transmembrane pore. - PubMed - NCBI
PubMed comprises more than 30 million citations for biomedical literature from MEDLINE, life science journals, and online books. Citations may include links to full-text content from PubMed Central and publisher web sites.
https://www.ncbi.nlm.nih.gov/pubmed/8943190?dopt=Abstract
Hemolysin unit | Article about hemolysin unit by The Free DictionaryHemolysin unit | Article about hemolysin unit by The Free Dictionary
Looking for hemolysin unit? Find out information about hemolysin unit. A unit for the standardization of blood serum Explanation of hemolysin unit
http://encyclopedia2.thefreedictionary.com/hemolysin+unit
DSpace at EWHA: Statin-Conferred Enhanced Cellular Resistance against Bacterial Pore-Forming Toxins in Airway Epithelial CellsDSpace at EWHA: Statin-Conferred Enhanced Cellular Resistance against Bacterial Pore-Forming Toxins in Airway Epithelial Cells
Statins are widely used to prevent cardiovascular disease. In addition to their inhibitory effects on cholesterol synthesis, statins have beneficial effects in patients with sepsis and pneumonia, although molecular mechanisms have mostly remained unclear. Using human airway epithelial cells as a proper in vitro model, we show that prior exposure to physiological nanomolar serum concentrations of simvastatin (ranging from 10-1,000 nM) confers significant cellular resistance to the cytotoxicity of pneumolysin, a pore-forming toxin and the main virulence factor of Streptococcus pneumoniae. This protection could be demonstrated with a different statin, pravastatin, or on a different toxin, a-hemolysin. Furthermore, through the use of gene silencing, pharmacological inhibitors, immunofluorescence microscopy, and biochemical and metabolic rescue approaches, we demonstrate that the mechanism of protection conferred by simvastatin at physiological nanomolar concentrations could be different from the ...
http://dspace.ewha.ac.kr/handle/2015.oak/217976
KAKEN - Research Projects | Assembly of Staphy lococcus aureus alpha-toxin on target membranes (KAKENHI-PROJECT-05670248)KAKEN - Research Projects | Assembly of Staphy lococcus aureus alpha-toxin on target membranes (KAKENHI-PROJECT-05670248)
Principal Investigator：TOMITA Toshio, Project Period (FY)：1993 - 1994, Research Category：Grant-in-Aid for General Scientific Research (C), Research Field：Bacteriology (including Mycology)
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-05670248/
Right Connections Travel - Temptations - Cancun - Lares, Stejnar, Elber and Anog Turks and caicos islands
Stature epilepticus induces a particular microglial activation state characterized by means of enhanced purinergic signaling. As a result, all workers are connected to the IMDB to count on pre-eminence information there currently executed tasks. Simvastatin inhibits inflammatory properties of staphylococci aureus alpha-toxin [url=http://andrewstark.com/intellectual/lesson3/document1/]purchase 160 mg super avana free shipping[/url] impotence caused by medication. Acquaint with parents to keep an eye on iron-containing supplements out-moded of the reach of young children in disposition to prevent accidental ingestion paramount to overdose or poisoning. Nurses obligated to educate parents and adolescents on jalopy, gun, and spa water safe keeping to preclude unintentional injuries. Ikeda, U, and Shimada, K (1999) [url=http://andrewstark.com/intellectual/lesson3/document9/]discount 100 mg nizagara fast delivery[/url] erectile dysfunction treatment exercises. DT provides maturing teams with a open ...
http://forum.rightconnectionstravel.com/topic.php?tid=615284
Hyperexpression of α-hemolysin explains enhanced virulence of sequence type 93 community-associated methicillin-resistant...Hyperexpression of α-hemolysin explains enhanced virulence of sequence type 93 community-associated methicillin-resistant...
These data demonstrate that hyperexpression of α-hemolysin mediates enhanced virulence in ST93 CA-MRSA, and additional control of exotoxin production, in particular α-hemolysin, mediated by regulatory systems other than agr have the potential to fine-tune virulence in CA-MRSA.
https://pubmed.ncbi.nlm.nih.gov/24512075/
IMMUNOLOGICAL STUDIES IN RELATION TO THE SUPRARENAL GLAND | JEMIMMUNOLOGICAL STUDIES IN RELATION TO THE SUPRARENAL GLAND | JEM
Hemolysin formation was studied in normal rats. It was found that a single intraperitoneal injection of 1 cc. of a 10 per cent suspension of red blood cells results in an optimum high hemolysin titer 5 days after injection. The titer gradually falls, the hemolysin disappearing from 14 to 18 days after the injection. Larger amounts are less effective in the production of amboceptor. Much smaller amounts produce correspondingly lower hemolysin titers, although the ratio is not mathematically proportional. Repeated injections of 1 cc. of a 10 per cent suspension of red blood cells at daily intervals or at intervals of 3 or 5 days for three injections do not increase the hemolysin titer over that resulting from a single injection. Furthermore, the curve of antibody formation following a single small intraperitoneal injec tion of red blood cells is not altered by subsequent injections of similar amounts.. ...
http://jem.rupress.org/content/47/5/713
talks.cam : Polymers through protein pores: single-molecule experiments with nucleic acids, polypeptides and polysaccharidestalks.cam : Polymers through protein pores: single-molecule experiments with nucleic acids, polypeptides and polysaccharides
If you have a question about this talk, please contact ma505.. When polymers move from one cellular compartment to another, they pass through protein pores. Nucleic acids, polypeptides and polysaccharides are all transported in this way, stimulating questions about the nature of the transported polymer (diameter, stiffness, branching, charge, charge distribution), the driving force (DV, DpH, refolding, binding) and how that driving force is coupled (direct coupling v diffusion/ ratchet). We have been investigating all three classes of biopolymer by current recording through individual transmembrane pores. We have not only made interesting fundamental discoveries about the translocation processes, but also found useful applications of our work, for example in nucleic acid sequencing and the discovery of antibacterial agents.. This talk is part of the Biophysical Seminars series.. ...
https://talks.cam.ac.uk/talk/index/69046
onkocet
In the work were used the clinical strains of E.coli, St. aureus, Ps.aeruginosa, P.vulgaris, Candida albicans, which have been the most typical agents of hospital infection. The suspended in the physiological solution daily cultures of microorganisms in the concentration to 106 units/ml on 1 standard loop (0,005 ml) sowed to dense nutrient medium - medium of Ploskirev, yolk- salt agar with the mannitol, blood agar, Endos medium. The sowing cultures processed by NO -CGF of apparatus PLASON during 1, 3 and 5 min from the distance of 6 cm by circular pendular motions. The processed cups incubated in aerothermostat with 37°C for 24 hours, after which was evaluated an increase in the microorganisms. In the check studies the sowings of cultures at the same dose processed in no way.. For checking the assumption about the possibility of NO influence on the factors of the aggression of the microorganisms, which are frequently determined by plasmids, was used the clinical hemolytic strain of E.coli ...
http://www.onkocet.eu/en/produkty-detail/32/1/
Potassium-inhibited processing of IL-1 beta in human monocytesPotassium-inhibited processing of IL-1 beta in human monocytes
Agents that deplete cells of K+ without grossly disrupting the plasma membrane were found to stimulate the cleavage of pro-interleukin (IL)-1 beta to mature IL-1 beta. Agents examined in this study included staphylococcal alpha-toxin and gramicidin, both of which selectively permeabilize plasma memb …
https://pubmed.ncbi.nlm.nih.gov/7737113/?dopt=Abstract
Download O Gene
Please date us via our download O gene database for more technology and re-download the care life formerly. malignancies work woken by this Heart. For more hand, are the exposures game.
http://www.jonathanshaw.com/back/ebooks/download-O-gene---Uma-hist%C3%B3ria-%C3%ADntima-2016/
Hfq regulates the expression of the thermostable direct hemolysin gene in Vibrio parahaemolyticus | BMC Microbiology | Full TextHfq regulates the expression of the thermostable direct hemolysin gene in Vibrio parahaemolyticus | BMC Microbiology | Full Text
The hfq gene is conserved in a wide variety of bacteria and Hfq is involved in many cellular functions such as stress responses and the regulation of gene expression. It has also been reported that Hfq is involved in bacterial pathogenicity. However, it is not clear whether Hfq regulates virulence in Vibrio parahaemolyticus. To evaluate this, we investigated the effect of Hfq on the expression of virulence-associated genes including thermostable direct hemolysin (TDH), which is considered to be an important virulence factor in V. parahaemolyticus, using an hfq deletion mutant. The production of TDH in the hfq deletion mutant was much higher than in the parental strain. Quantification of tdh promoter activity and mRNA demonstrated that transcription of the tdh gene was up-regulated in the mutant strain. The hfq-complemented strain had a normal (parental) amount of tdh expression. The transcriptional activity of tdhA was particularly increased in the mutant strain. These results indicate that Hfq is
https://0-bmcmicrobiol-biomedcentral-com.brum.beds.ac.uk/articles/10.1186/1471-2180-8-155
Carboxy terminal region of haemolysin of Aeromonas sobria triggers dimerization<...
TY - JOUR. T1 - Carboxy terminal region of haemolysin of Aeromonas sobria triggers dimerization. AU - Nomura, Tomohiko. AU - Hamashima, Hajime. AU - Okamoto, Keinosuke. PY - 2000/1. Y1 - 2000/1. N2 - Haemolysin of Aeromonas sobria is released into the culture supernatant in the form of prohaemolysin. Removal of a 42 amino acid peptide at the carboxy-terminal end converts prohaemolysin into mature haemolysin. As the role of the peptide removed from the mature haemolysin has not been studied, we mutated the haemolysin genes to delete several amino acid residues from the carboxy terminus, expressed the mutant genes in A. sobria and analysed the haemolysins produced. Deletion of more than three amino acid residues significantly reduced the efficiency of secretion of haemolysin into the culture supernatant. Mutant haemolysins with deletion of 10 amino acids were easily degraded in cells. Furthermore, cross-linking experiments indicated that the haemolysins dimerize in cells, and thus dimerized ...
https://okayama.pure.elsevier.com/en/publications/carboxy-terminal-region-of-haemolysin-of-aeromonas-sobria-trigger
ABCC2 is associated with Bacillus thuringiensis Cry1Ac toxin oligomerization and membrane insertion in diamondback moth<...
TY - JOUR. T1 - ABCC2 is associated with Bacillus thuringiensis Cry1Ac toxin oligomerization and membrane insertion in diamondback moth. AU - Ocelotl,Josue. AU - Sánchez,Jorge. AU - Gómez,Isabel. AU - Tabashnik,Bruce E.. AU - Bravo,Alejandra. AU - Soberón,Mario. PY - 2017/12/1. Y1 - 2017/12/1. N2 - Cry1A insecticidal toxins bind sequentially to different larval gut proteins facilitating oligomerization, membrane insertion and pore formation. Cry1Ac interaction with cadherin triggers oligomerization. However, a mutation in an ABC transporter gene (ABCC2) is linked to Cry1Ac resistance in Plutella xylostella. Cry1AcMod, engineered to lack helix α-1, was able to form oligomers without cadherinbinding and effectively countered Cry1Ac resistance linked to ABCC2. Here we analyzed Cry1Ac and Cry1AcMod binding and oligomerization by western blots using brush border membrane vesicles (BBMV) from a strain of P. xylostella susceptible to Cry1Ac (Geneva 88) and a strain with resistance to Cry1Ac ...
https://arizona.pure.elsevier.com/en/publications/abcc2-is-associated-with-bacillus-thuringiensis-cry1ac-toxin-olig
Alteration of epithelial cell lysosomal integrity induced by bacterial cholesterol-dependent cytolysins. - Institut PasteurAlteration of epithelial cell lysosomal integrity induced by bacterial cholesterol-dependent cytolysins. - Institut Pasteur
Bacterial pathogens can interfere during infection with host cell organelles, such as mitochondria, the endoplasmic reticulum-Golgi system or nuclei. As important cellular functions are often compartmentalized in these organelles, their targeting allows pathogens to manipulate key host functions during infection. Here, we identify lysosomes as a new class of organelles targeted by the pathogenic bacterium Listeria monocytogenes. We demonstrate that extracellular Listeria, via secretion of the pore-forming toxin listeriolysin O, alters lysosomal integrity in epithelial cells but not in macrophages. Listeriolysin O induces lysosomal membrane permeabilization and release of lysosomal content, such as cathepsins proteases, which remain transiently active in the host cytosol. We furthermore show that other bacterial pore-forming toxins, such as perfringolysin O and pneumolysin, also induce lysosomes alteration. Together, our data unveil a novel activity of bacterial cholesterol-dependent cytolysins.
https://hal-pasteur.archives-ouvertes.fr/pasteur-01574071
Structural stability of Bacillus thuringiensis delta-endotoxin homolog-scanning mutants determined by susceptibility to...Structural stability of Bacillus thuringiensis delta-endotoxin homolog-scanning mutants determined by susceptibility to...
Structural stability of Bacillus thuringiensis delta-endotoxin homolog-scanning mutants determined by susceptibility to proteases
http://www.readabstracts.com/Biological-sciences/Structural-stability-of-Bacillus-thuringiensis-delta-endotoxin-homolog-scanning-mutants-determined-b.html
Bacillus thuringiensis Cry1Ac protoxin is a potent systemic and mucosal adjuvant<...
TY - JOUR. T1 - Bacillus thuringiensis Cry1Ac protoxin is a potent systemic and mucosal adjuvant. AU - Vazquez-Padron, Roberto I. AU - Moreno-Fierros, L.. AU - Neri-Bazán, L.. AU - De La Riva, G. A.. AU - López-Revilla, Rubén. PY - 1999/7/5. Y1 - 1999/7/5. N2 - Recently we demonstrated that recombinant Cry1Ac protoxin from Bacillus thuringiensis is a potent systemic and mucosal immunogen. In this study we compared the adjuvant effects of Cry1Ac and cholera toxin (CT) for the hepatitis B surface antigen (HBsAg) and bovine serum albumin (BSA). The antibody responses of intestinal secretions and serum were determined by ELISA in Balb/c mice immunized through the intragastric (IG) or intraperitoneal (IP) routes. When HBsAg was administered via IG, the anti- HBsAg intestinal response was not enhanced by either Cry1Ac or CT, whereas via IP Cry1Ac increased the anti-HBsAg intestinal immunoglobulin (Ig)G response and CT increased the intestinal IgA and IgM responses. Serum anti- BSA antibodies ...
https://miami.pure.elsevier.com/en/publications/bacillus-thuringiensis-cry1ac-protoxin-is-a-potent-systemic-and-m
Toxins | Free Full-Text | Distinct Neurotoxicity Profile of Listeriolysin O from Listeria monocytogenesToxins | Free Full-Text | Distinct Neurotoxicity Profile of Listeriolysin O from Listeria monocytogenes
Cholesterol-dependent cytolysins (CDCs) are protein toxins that originate from Gram-positive bacteria and contribute substantially to their pathogenicity. CDCs bind membrane cholesterol and build prepores and lytic pores. Some effects of the toxins are observed in non-lytic concentrations. Two pathogens, Streptococcus pneumoniae and Listeria monocytogenes, cause fatal bacterial meningitis, and both produce toxins of the CDC family-pneumolysin and listeriolysin O, respectively. It has been demonstrated that pneumolysin produces dendritic varicosities (dendrite swellings) and dendritic spine collapse in the mouse neocortex, followed by synaptic loss and astrocyte cell shape remodeling without elevated cell death. We utilized primary glial cultures and acute mouse brain slices to examine the neuropathological effects of listeriolysin O and to compare it to pneumolysin with identical hemolytic activity. In cultures, listeriolysin O permeabilized cells slower than pneumolysin did but still initiated non
http://www.mdpi.com/2072-6651/9/1/34
Interaction analyses of Bacillus thuringiensis Cry1A toxins with two aminopeptidases purified from Gypsy moth midgut brush...Interaction analyses of Bacillus thuringiensis Cry1A toxins with two aminopeptidases purified from Gypsy moth midgut brush...
Interaction analyses of Bacillus thuringiensis Cry1A toxins with two aminopeptidases purified from Gypsy moth midgut brush border membranes
https://publications-cnrc.canada.ca/eng/view/object/?id=93858d7a-7d46-42cf-8019-9d2d68a5a318
A Bacillus thuringiensis subsp. israelensis gene encoding a 125-kilodalton larvicidal polypeptide is associated with inverted...A Bacillus thuringiensis subsp. israelensis gene encoding a 125-kilodalton larvicidal polypeptide is associated with inverted...
A gene encoding a 125-kilodalton (kDa) mosquitocidal delta-endotoxin was cloned from the 72-MDa resident plasmid of Bacillus thuringiensis subsp. israelensis.
https://research.pasteur.fr/en/publication/a-bacillus-thuringiensis-subsp-israelensis-gene-encoding-a-125-kilodalton-larvicidal-polypeptide-is-associated-with-inverted-repeat-sequences/
Komposisi media dan teknologi sederhana produksi bioinsektisida dari bacillus thuringiensis subsp kurstaki - KMS IPB ...Komposisi media dan teknologi sederhana produksi bioinsektisida dari bacillus thuringiensis subsp kurstaki - KMS IPB ...
Institut Pertanian Bogor (IPB) (2001) Komposisi media dan teknologi sederhana produksi bioinsektisida dari bacillus thuringiensis subsp kurstaki. S00200100120. ...
http://kms.ipb.ac.id/407/
Listeriolysin O - WikipediaListeriolysin O - Wikipedia
Listeriolysin O (LLO) is a hemolysin produced by the bacterium Listeria monocytogenes, the pathogen responsible for causing listeriosis. The toxin may be considered a virulence factor, since it is crucial for the virulence of L. monocytogenes. Listeriolysin O is a non-enzymatic, cytolytic, thiol-activated, cholesterol-dependent, pore-forming toxin protein; hence, it is activated by reducing agents and inhibited by oxidizing agents. However, LLO differs from other thiol-activated toxins, since its cytolytic activity is maximized at a pH of 5.5. By maximizing activity at a pH of 5.5, LLO is selectively activated within the acidic phagosomes (average pH ~ 5.9) of cells that have phagocytosed L. monocytogenes. After LLO lyses the phagosome, the bacterium escapes into the cytosol, where it can grow intracellularly. Upon release from the phagosome, the toxin has reduced activity in the more basic cytosol. Hence, LLO permits L. monocytogenes to escape from phagosomes into the cytosol without damaging ...
https://en.wikipedia.org/wiki/Listeriolysin_O
Genetic analysis of the pAD1 hemolysin/bacteriocin determinant in Enterococcus faecalis: Tn917 insertional mutagenesis and...Genetic analysis of the pAD1 hemolysin/bacteriocin determinant in Enterococcus faecalis: Tn917 insertional mutagenesis and...
Thirty-seven nonhemolytic/nonbacteriocinogenic mutations in Enterococcus (Streptococcus) faecalis plasmid pAD1 were generated by Tn917 insertion. All were found to belong to one of two complementation classes. Each class of mutants secreted either hemolysin/bacteriocin (Hly/Bac) component A or L into the culture medium. DNA encoding Hly/Bac was cloned in Escherichia coli in which both components of the hemolysin were expressed individually and collectively. The region encoding components A and L was further defined by deletion analysis and physically mapped. A total of approximately 8.4 kilobases of pAD1 DNA were observed to be required for hemolysin expression. Hly/Bac activity of the wild-type and the inactive L substance was observed to be heat stable. Active Hly/Bac resulting from incubating separately secreted components A and L was also found to be heat stable. The results indicate that component A activates component L and that activated component L possesses the Hly/Bac activity. ...
https://jb.asm.org/content/172/1/155
Functional assembly of two membrane-binding domains in listeriolysin O, the cytolysin of Listeria monocytogenes | Microbiology...Functional assembly of two membrane-binding domains in listeriolysin O, the cytolysin of Listeria monocytogenes | Microbiology...
Listeriolysin O (LLO) is a major virulence factor secreted by the pathogenic Listeria monocytogenes and acts as pore-forming cytolysin. Based on sequence similarities between LLO and perfringolysin (PFO), the cytolysin from Clostridium perfringens of known crystallographic structure, two truncated LLO proteins were produced: LLO-d123, comprising the first three predicted domains, and LLO-d4, the last C-terminal domain. The two proteins were efficiently secreted into the culture supernatant of L. monocytogenes and were able to bind to cell membranes. Strikingly, when expressed simultaneously, the two secreted domains LLO-d123 and LLO-d4 reassembled into a haemolytically active form. Two in-frame linker insertions were generated in the hinge region between the d123 and d4 domains. In both cases, the insertion created a major cleavage site for proteolytic degradation and abolished cytolytic activity, which might suggest that the region connecting d123 and d4 participates in the interaction between the two
https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-147-10-2679
Bacillus thuringiensis - VikipediBacillus thuringiensis - Vikipedi
Bacillus thuringiensis (Bt) genellikle biyolojik pestisit olarak kullanılan, Gram-pozitif, toprakta yaşayan bir bakteridir.[1] B. thuringiensis çeşitli güve ve kelebek türlerinin tırtıllarının bağırsaklarında, yaprak yüzeylerinda, sucul ortamlarda, hayvan dışkısında, böcek popülasyonunun yoğun olduğu ortamlarda, un değirmenleri ve tahıl depolama tesislerinde doğal olarak bulunmaktadır. Sporlanma sırasında, birçok Bacillus thuringiensis suşunun δ-endotoksinleri denilen kristal proteinleri (proteinli inklüzyonlar) üreterek böcekleri öldürdüğü görülmüş bu da böcek öldürücü maddeler olarak kullanımına yol açmıştır. Birçok kristal üreten Bacillus thuringiensis suşu olsa da hepsinin böcek öldürücü özellikleri yoktur. ...
https://tr.wikipedia.org/wiki/Bacillus_thuringiensis
Bacillus thuringiensis subspecies kurstaki strain EG7841 solids, spores and Lepidopteran active toxins | GreenbookBacillus thuringiensis subspecies kurstaki strain EG7841 solids, spores and Lepidopteran active toxins | Greenbook
Labels related to ingredient - Bacillus thuringiensis subspecies kurstaki strain EG7841 solids, spores and Lepidopteran active toxins.
https://www.greenbook.net/ingredients/bacillus-thuringiensis-subspecies-kurstaki-strain-eg7841-solids-spores-and-lepidopteran
Team:NCTU Formosa/Cry production - 2010.igem.orgTeam:NCTU Formosa/Cry production - 2010.igem.org
Bacillus thuringiensis, which is a Gram-positive bacteria. It can produce insecticidal crystal proteins(ICP) which are proteolytically processed by gut proteases into the activated δ-endotoxins. The toxins activated by gut proteases bind to specific binding sites on the brush border membranes of insect midgut epithelial cells. The conformational change in the toxin molecules triggers the insertion of their pore-forming domain into the membrane. Finally, colloid-osmotic swelling and lysis of the cell result in the death of the larvae. Cry11Aa protein is one of the crystal protein coded in Bacillus thuringiensis subsp. Israelensis and it is highly toxic to certain dipteran larvae, such as Aedes, and Anopheles larvae. The length of Cry11Aa sequence is about 1.9Kb. In our project, the cry weapon system produce crystal protein, targetting the wrigglers, larvae of mosquitoes. It is controlled by the tetR-repressible promoter PtetR(BBa_R0040), which in turn is regulated by a temperature control ...
http://2010.igem.org/wiki/index.php?title=Team:NCTU_Formosa/Cry_production&diff=prev&oldid=203428
Team:NCTU Formosa/Cry production - 2010.igem.orgTeam:NCTU Formosa/Cry production - 2010.igem.org
Bacillus thuringiensis, which is a Gram-positive bacteria. It can produce insecticidal crystal proteins(ICP) which are proteolytically processed by gut proteases into the activated δ-endotoxins. The toxins activated by gut proteases bind to specific binding sites on the brush border membranes of insect midgut epithelial cells. The conformational change in the toxin molecules triggers the insertion of their pore-forming domain into the membrane. Finally, colloid-osmotic swelling and lysis of the cell result in the death of the larvae. Cry11Aa protein is one of the crystal protein coded in Bacillus thuringiensis subsp. Israelensis and it is highly toxic to certain dipteran larvae, such as Aedes, and Anopheles larvae. The length of Cry11Aa sequence is about 1.9Kb. In our project, the cry weapon system produce crystal protein, targetting the wrigglers, larvae of mosquitoes. It is controlled by the tetR-repressible promoter PtetR(BBa_R0040), which in turn is regulated by a temperature control ...
http://2010.igem.org/wiki/index.php?title=Team:NCTU_Formosa/Cry_production&direction=next&oldid=201557
Antiproliferative protein from Bacillus thuringiensis var. thuringiensis - Research Development FoundationAntiproliferative protein from Bacillus thuringiensis var. thuringiensis - Research Development Foundation
The present invention provides an isolated and purified protein derived from Bacillus thuringiensis subspecies thuringiensis, having a molecular weight of approximately 20 kDa of SDS-PAGE, said protei
http://www.freepatentsonline.com/5977058.html
Exploring the role of the CTL epitope region of listeriolysin O in the pathogenesis of Listeria monocytogenes | Microbiology...Exploring the role of the CTL epitope region of listeriolysin O in the pathogenesis of Listeria monocytogenes | Microbiology...
Listeria monocytogenes is a facultative intracellular bacterial pathogen responsible for severe opportunistic infections in humans and animals. The secreted cholesterol-dependent cytolysin, listeriolysin O (LLO), mediates phagosomal escape and allows bacterial growth in the cytosol of infected cells. In order to identify new LLO determinants participating in bacterial pathogenesis, this study focused on a major target of LLO proteolytic cleavage in vitro, the CTL epitope region (residues 91-99). Mutations were generated by site-directed mutagenesis in the epitope or in the two clusters of positive charges flanking the epitope. Two LLO mutants (a single mutation K103A and a double mutation R89G, K90G) were normally and stably secreted by L. monocytogenes. In contrast, a mutant carrying four amino acid substitutions in the epitope itself (Y92K, D94A, E97K, Y98F) was highly susceptible to proteolytic degradation. While these three LLO mutant proteins showed a reduced haemolytic activity, they all promoted
https://www.microbiologyresearch.org/content/journal/micro/10.1099/mic.0.28754-0
Cry64Ba and Cry64Ca, Two ETX/MTX2-Type Bacillus thuringiensis Insecticidal Proteins Active against Hemipteran Pests | Applied...Cry64Ba and Cry64Ca, Two ETX/MTX2-Type Bacillus thuringiensis Insecticidal Proteins Active against Hemipteran Pests | Applied...
Cry proteins are crucial virulence factors of Bt strains. They have been subdivided into several groups, such as three-domain, ETX/MTX-like, and Bin-like toxins (12). Here, we report two ETX/MTX type Cry proteins from Bt strain 1012. The Cry64Ba and Cry64Ca proteins presented here were successfully expressed in Bt HD73− only when they were cloned simultaneously as one operon. The coexpression protein product (Cry64Ba/Cry64Ca) showed efficient and high insecticidal activity against important sap-sucking pests (Table 1). It is unclear why the two components are essential for their high expression. One possibility is that the cotranscription of these two genes may promote the stability of the mRNA. Another possibility is that the interaction of the two proteins may stabilize their physical and chemical properties or confer resistance to endogenous protease digestion. Gel filtration chromatography showed that both Cry64Ba and Cry64Ca proteins form a complex with a molecular size higher than 66 kDa ...
https://aem.asm.org/content/84/3/e01996-17
cry6Aa - Pesticidal crystal protein Cry6Aa - Bacillus thuringiensis - cry6Aa gene & proteincry6Aa - Pesticidal crystal protein Cry6Aa - Bacillus thuringiensis - cry6Aa gene & protein
Pore-forming toxin with nematicidal activity (PubMed:26795495, PubMed:27576487). In infected C.elegans, induces an increase in intracellular Ca(2+) resulting in necrosis of host intestinal cells (PubMed:26795495). Also, induces the expression of aspartic protease asp-1 (PubMed:26795495).
https://www.uniprot.org/uniprot/Q45757
Bacillus thuringiensis Berliner ATCC ® 35646D-5™Bacillus thuringiensis Berliner ATCC ® 35646D-5™
Bacillus thuringiensis ATCC ® 35646D-5™ Designation: Genomic DNA from Bacillus thuringiensis Strain USDA H522 TypeStrain=False Application:
https://www.atcc.org/en/Products/Cells_and_Microorganisms/Bacteria/Bacterial_Nucleic_Acids/35646D-5.aspx
Fate and transport of Cry1Ab from transgenic Bacillus thuringiensis co by Katherine E. StrainFate and transport of Cry1Ab from transgenic Bacillus thuringiensis co by Katherine E. Strain
AN ABSTRACT OF THE THESIS OF Katherine E. Strain, for the Masters degree in Zoology, presented on October 21, 2014, at Southern Illinois University Carbondale. TITLE: FATE AND TRANSPORT OF CRY1AB FROM TRANSGENIC BACILLUS THURINGIENSIS CORN IN AN AGRICULTURAL FIELD AND AQUATIC MICROCOSMS MAJOR PROFESSOR: Dr. Michael Lydy, Ph.D. Genetically-modified crops expressing insecticidal crystalline proteins derived from a soil bacterium, Bacillus thuringiensis (Bt), were commercialized almost two decades ago as a means to combat agricultural pests. The Bt proteins are highly specific and only lethal upon ingestion, limiting the scope of toxicity to target insects. However, evidence for risk to non-target organisms and negative public perceptions on the use of Bt crops has caused controversy surrounding their use. The objective of this research was to monitor the fate and transport of a Bt protein, Cry1Ab, in a large-scale agricultural field and in aquatic microcosms. Quantitative methods were validated
https://opensiuc.lib.siu.edu/theses/1596/
Bacillus thuringiensis Market in Brazil Overview 2021-2027: Diversely changing Market Trends with Potential Business Growth -...Bacillus thuringiensis Market in Brazil Overview 2021-2027: Diversely changing Market Trends with Potential Business Growth -...
Latest research report on Bacillus thuringiensis Market in Brazil now available at high quality database of ReportsnReports.com with market size, share, trends, competitive and statistical analysis. An exclusive data offered in this report is collected by research and industry experts team.. Get Free Sample Research Report @ https://www.reportsnreports.com/contacts/requestsample.aspx?name=3520783. Bacillus thuringiensis (or Bt) is a Gram-positive, soil-dwelling bacterium, commonly used as a biological pesticide. B. thuringiensis also occurs naturally in the gut of caterpillars of various types of moths and butterflies, as well on leaf surfaces, aquatic environments, animal feces, insect-rich environments, and flour mills and grain-storage facilities ...
https://www.marketoptimizer.org/bacillus-thuringiensis-market-in-brazil-overview-2021-2027-diversely-changing-market-trends-with-potential-business-growth/
In vitro proteolytic processing and activation of the recombinant precursor of El Tor cytolysin/hemolysin (pro-HlyA) of Vibrio...In vitro proteolytic processing and activation of the recombinant precursor of El Tor cytolysin/hemolysin (pro-HlyA) of Vibrio...
Vibrio cholerae produces a cytolytic toxin named El Tor cytolysin/hemolysin which is encoded by the hlyA gene. This cytolysin is produced as a 79-kDa precursor form (pro-HlyA) into the culture supernatant after cleavage of the signal peptide of the hlyA product (prepro-HlyA). The pro-HlyA is then processed to a 65-kDa mature cytolysin (mature HlyA) after cleavage of the 15-kDa N-terminal peptide (pro region) of the 79-kDa precursor, usually at the bond between Ala-157 and Asn-158. We investigated whether proteases could process the recombinant 79-kDa pro-HlyA to the 65-kDa mature HlyA. We observed that the soluble hemagglutinin/ protease (HA/protease; a major protease of V. cholerae), trypsin, alpha-chymotrypsin, subtilisin BPN, papain, and thermolysin all processed the pro-HlyA to the 65-kDa mature form of the protein. Along with this, the protease-processed HlyA showed drastically increased hemolytic activity. The N-terminal amino acid of the mature form of cytolysin generated by HA/protease ...
https://iai.asm.org/content/64/11/4655?ijkey=46bbe5db3c6ff2d53f031ab7659e95a1bfcf8fa0&keytype2=tf_ipsecsha
CONFERENCE ON BIOTECHNOLOGY OF BACILLUS THURINGIENSISCONFERENCE ON BIOTECHNOLOGY OF BACILLUS THURINGIENSIS
First Announcement THE PACIFIC RIM CONFERENCE ON BIOTECHNOLOGY OF BACILLUS THURINGIENSIS AND ITS IMPACT TO THE ENVIRONMENT October 17 - 21, 1994 In order to promote the use of BACILLUS THURINGIENSIS (B.t.)-based insecticides in the Pacific rim region, the conference is being organized by a group of scientists from the universities, government institutions, and B.t. industries from Taiwan, Japan, and the U.S.A. The conference will be held in the Academic Activity Center, Academia Sinica, Taipei, Taiwan, which can accommodate around 200 participants. In addition to the speakers from the U.S.A. and Canada, the conference organizers plan to invite as many speakers as possible from the Asia- Pacific area. The goals of the conference are: * To promote the biotechnolgy of B.t. for the agricultural industry; to exchange the latest information on strain isolation and identification; to review the current topics on the molecular biology of the insecticidal proteins, transgenic plants, resistance ...
http://www.bio.net/bionet/mm/bionews/1993-September/000702.html
Toxins | Free Full-Text | The Vip3Ag4 Insecticidal Protoxin from Bacillus thuringiensis Adopts A Tetrameric Configuration That...Toxins | Free Full-Text | The Vip3Ag4 Insecticidal Protoxin from Bacillus thuringiensis Adopts A Tetrameric Configuration That...
The Vip3 proteins produced during vegetative growth by strains of the bacterium Bacillus thuringiensis show insecticidal activity against lepidopteran insects with a mechanism of action that may involve pore formation and apoptosis. These proteins are promising supplements to our arsenal of insecticidal proteins, but the molecular details of their activity are not understood. As a first step in the structural characterisation of these proteins, we have analysed their secondary structure and resolved the surface topology of a tetrameric complex of the Vip3Ag4 protein by transmission electron microscopy. Sites sensitive to proteolysis by trypsin are identified and the trypsin-cleaved protein appears to retain a similar structure as an octomeric complex comprising four copies each of the ~65 kDa and ~21 kDa products of proteolysis. This processed form of the toxin may represent the active toxin. The quality and monodispersity of the protein produced in this study make Vip3Ag4 a candidate for more detailed
http://www.mdpi.com/2072-6651/9/5/165
What is Bacillus Thuringiensis (Bt)? - Definition from MaximumYieldWhat is Bacillus Thuringiensis (Bt)? - Definition from MaximumYield
Bacillus Thuringiensis Bt Definition - Bacillus thuringiensis (Bt) is a type of spore-forming bacterium. Bt occurs naturally in the soil of all types of...
https://www.maximumyield.com/definition/732/bacillus-thuringiensis-bt
The Vip3Ag4 insecticidal protoxin from bacillus thuringiensis adopts a tetrameric configuration that is maintained on...The Vip3Ag4 insecticidal protoxin from bacillus thuringiensis adopts a tetrameric configuration that is maintained on...
The Vip3 proteins produced during vegetative growth by strains of the bacterium Bacillus thuringiensis show insecticidal activity against lepidopteran insects with a mechanism of action that may involve pore formation and apoptosis. These proteins are promising supplements to our arsenal of insecticidal proteins, but the molecular details of their activity are not understood. As a first step in the structural characterisation of these proteins, we have analysed their secondary structure and resolved the surface topology of a tetrameric complex of the Vip3Ag4 protein by transmission electron microscopy. Sites sensitive to proteolysis by trypsin are identified and the trypsin-cleaved protein appears to retain a similar structure as an octomeric complex comprising four copies each of the ~65 kDa and ~21 kDa products of proteolysis. This processed form of the toxin may represent the active toxin. The quality and monodispersity of the protein produced in this study make Vip3Ag4 a candidate for more ...
http://academica-e.unavarra.es/xmlui/handle/2454/26151
Mikrobiologiske plantebeskyttelsesmidlers skæbne i mave-tarm kanalen - Studier af Bacillus thuringiensis</em>...Mikrobiologiske plantebeskyttelsesmidlers skæbne i mave-tarm kanalen - Studier af Bacillus thuringiensis</em>...
TY - RPRT. T1 - Mikrobiologiske plantebeskyttelsesmidlers skæbne i mave-tarm kanalen - Studier af Bacillus thuringiensis. AU - Wilcks, Andrea. AU - Hansen, B. M.. AU - Hendriksen, N. B.. AU - Ørum-Smidt, Lasse. AU - Andrup, L.. AU - Licht, Tine Rask. PY - 2006. Y1 - 2006. M3 - Rapport. BT - Mikrobiologiske plantebeskyttelsesmidlers skæbne i mave-tarm kanalen - Studier af Bacillus thuringiensis. PB - Miljøstyrelsen. ER - ...
https://orbit.dtu.dk/en/publications/mikrobiologiske-plantebeskyttelsesmidlers-sk%C3%A6bne-i-mave-tarm-kana
Genetically engineered (modified) crops ( Bacillus thuringiensis crops) and the world controversy on their safety | Egyptian...Genetically engineered (modified) crops ( Bacillus thuringiensis crops) and the world controversy on their safety | Egyptian...
Bacillus thuringiensis (Bt) crops are plants genetically engineered (modified) to contain the endospore (or crystal) toxins of the bacterium, Bt to be resistant to certain insect pests. In 1995, the Environmental Protection Agency (EPA) in USA approved the commercial production and distribution of the Bt crops: corn, cotton, potato, and tobacco. Currently, the most common Bt crops are corn and cotton. The crystal, referred to as Cry toxins, is proteins formed during sporulation of some Bt strains and aggregate to form crystals. Such Cry toxins are toxic to specific species of insects belongs to orders: Lepidoptera, Coleoptera, Hymenoptera, Diptera, and Nematoda. In 2016, the total world area cultivated with genetically modified crops (GM crops) reached about 185 million ha. This review shows that there is a worldwide controversy about the safety of Bt crops to the environment and mammals. Some researchers support the cultivation of Bt crops depending upon the results of their laboratory and field
https://ejbpc.springeropen.com/articles/10.1186/s41938-018-0051-2
Duration learning for analysis of nanopore ionic current blockades | BMC Bioinformatics | Full TextDuration learning for analysis of nanopore ionic current blockades | BMC Bioinformatics | Full Text
Ionic current blockade signal processing, for use in nanopore detection, offers a promising new way to analyze single molecule properties, with potential implications for DNA sequencing. The alpha-Hemolysin transmembrane channel interacts with a translocating molecule in a nontrivial way, frequently evidenced by a complex ionic flow blockade pattern. Typically, recorded current blockade signals have several levels of blockade, with various durations, all obeying a fixed statistical profile for a given molecule. Hidden Markov Model (HMM) based duration learning experiments on artificial two-level Gaussian blockade signals helped us to identify proper modeling framework. We then apply our framework to the real multi-level DNA hairpin blockade signal. The identified upper level blockade state is observed with durations that are geometrically distributed (consistent with an a physical decay process for remaining in any given state). We show that mixture of convolution chains of geometrically distributed
https://bmcbioinformatics.biomedcentral.com/articles/10.1186/1471-2105-8-S7-S14
Occurrence of cry Genes in Bacillus thuringiensis (Bt) Isolates Recovered from Phylloplanes of Crops Growing in the New Delhi...Occurrence of cry Genes in Bacillus thuringiensis (Bt) Isolates Recovered from Phylloplanes of Crops Growing in the New Delhi...
Abstract: Occurrence of cry Genes in Bacillus thuringiensis (Bt) Isolates Recovered from Phylloplanes of Crops Growing in the New Delhi Region of India and Toxicity Towards Diamond-back Moth (Plutella xylostella)
https://scialert.net/abstract/?doi=jbs.2013.463.473
Protection of experimentally infected pigs by suilysin, the thiol-activated haemolysin of Streptococcus suis | Veterinary Record
Three groups of three pigs were vaccinated either with vaccine VAC-SLY, containing purified suilysin derived from Streptococcus suis strain P1/7 (serotype 2), or with vaccine VAC-SCF, containing most of the other extracellular antigens produced by strain P1/7 (but essentially free from suilysin), or with a placebo vaccine. The pigs were vaccinated twice at four weeks and six weeks of age and were challenged intravenously with S suis strain P1/7 at eight weeks of age. On the day of challenge, only the VAC-SLY vaccinated pigs showed an increase in haemolysin neutralisation titre. After challenge the placebo vaccinated pigs developed severe clinical signs characterised by lameness involving several joints, a depressed appearance, high temperatures and/or neurological signs. The VAC-SCF vaccinated pigs showed the same clinical signs but less severely. The VAC-SLY vaccinated pigs were the least affected and showed only mild signs which subsided more quickly than those of the other groups. A post ...
https://veterinaryrecord.bmj.com/content/139/10/225
جداسازی جدایه‌های بومی ‌باکتری Bacillus thuringiensis Berliner از خاک‌های زراعی ایران
اولین قدم به عنوان پایه و اساس تحقیق روی باکتریBacillus thuringiensis ، جداسازی جدایه‌های بومی ‌و نگهداری آن‌ها به عنوان بانک ژن این باکتری است. برای این کار در سال‌های 1380-1378 تعداد 2234 نمونه خاک زراعی از کلیه استان‌های ایران جمع‌آوری شد. با استفاده از روش انور حسین و همکاران با اندکی تغییر تعداد 445,28 جدایه باکتری تشکیل‌دهنده اسپور و در نهایت 128 جدایه باکتری B. thuringiensis جداسازی شد. بیشترین تعداد جدایه‌های این باکتری نسبت به تعداد نمونه جمع‌آوری شده از خاک مزارع پنبه، چغندرقند، برنج و دانه‌های روغنی (آفتابگردان و کلزا) بدست آمد و کمترین تعداد از مزارع صیفی و سبزی
https://www.agroecojournal.com/article_514747.html
Cytolysin - WikipediaCytolysin - Wikipedia
Cytolysin refers to the substance secreted by microorganisms, plants or animals that is specifically toxic to individual cells, in many cases causing their dissolution through lysis. Cytolysins that have a specific action for certain cells are named accordingly. For instance, the cytolysins responsible for the destruction of red blood cells, thereby liberating hemoglobins, are named hemolysins, and so on. Cytolysins may be involved in immunity as well as in venoms. Hemolysin is also used by certain bacteria, such as Listeria monocytogenes, to disrupt the phagosome membrane of macrophages and escape into the cytoplasm of the cell. The term Cytolysin or Cytolytic toxin was first introduced by Alan Bernheimer to describe membrane damaging toxins (MDTs) that have cytolytic effects to cells. The first kind of cytolytic toxin discovered have hemolytic effects on erythrocytes of certain sensitive species, such as Human. For this reason Hemolysin was first used to describe any MDTs. In the 1960s ...
https://en.wikipedia.org/wiki/Cytolysin
Bacillus thuringiensis subspecies Israelensis strain BK solids, spores, and insecticidal toxins, ATCC number 35646, - toxicity,...Bacillus thuringiensis subspecies Israelensis strain BK solids, spores, and insecticidal toxins, ATCC number 35646, - toxicity,...
Data on 6,500 pesticides, insecticides and herbicides including toxicity, water pollution, ecological toxicity, uses and regulatory status.
http://www.pesticideinfo.org/Detail_Chemical.jsp?Rec_Id=PC41337
SWISSPROT: Q3Z4T9 SHISS
ID Q3Z4T9_SHISS Unreviewed; 72 AA. AC Q3Z4T9; DT 27-SEP-2005, integrated into UniProtKB/TrEMBL. DT 27-SEP-2005, sequence version 1. DT 08-MAY-2019, entry version 65. DE SubName: Full=Haemolysin expression modulating protein {ECO:0000313,EMBL:AAZ87223.1}; GN Name=hha {ECO:0000313,EMBL:AAZ87223.1}; GN OrderedLocusNames=SSON_0447 {ECO:0000313,EMBL:AAZ87223.1}; OS Shigella sonnei (strain Ss046). OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales; OC Enterobacteriaceae; Shigella. OX NCBI_TaxID=300269 {ECO:0000313,EMBL:AAZ87223.1, ECO:0000313,Proteomes:UP000002529}; RN [1] {ECO:0000313,EMBL:AAZ87223.1, ECO:0000313,Proteomes:UP000002529} RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=Ss046 {ECO:0000313,EMBL:AAZ87223.1, RC ECO:0000313,Proteomes:UP000002529}; RX PubMed=16275786; DOI=10.1093/nar/gki954; RA Yang F., Yang J., Zhang X., Chen L., Jiang Y., Yan Y., Tang X., RA Wang J., Xiong Z., Dong J., Xue Y., Zhu Y., Xu X., Sun L., Chen S., RA Nie H., Peng J., Xu J., Wang Y., ...
http://pbil.univ-lyon1.fr/cgi-bin/acnuc-search-ac?query=AAZ87223&db=SWISSPROT&ident=ACNUC7421
SWISSPROT: Q3Z4T9 SHISS
ID Q3Z4T9_SHISS Unreviewed; 72 AA. AC Q3Z4T9; DT 27-SEP-2005, integrated into UniProtKB/TrEMBL. DT 27-SEP-2005, sequence version 1. DT 08-MAY-2019, entry version 65. DE SubName: Full=Haemolysin expression modulating protein {ECO:0000313,EMBL:AAZ87223.1}; GN Name=hha {ECO:0000313,EMBL:AAZ87223.1}; GN OrderedLocusNames=SSON_0447 {ECO:0000313,EMBL:AAZ87223.1}; OS Shigella sonnei (strain Ss046). OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales; OC Enterobacteriaceae; Shigella. OX NCBI_TaxID=300269 {ECO:0000313,EMBL:AAZ87223.1, ECO:0000313,Proteomes:UP000002529}; RN [1] {ECO:0000313,EMBL:AAZ87223.1, ECO:0000313,Proteomes:UP000002529} RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=Ss046 {ECO:0000313,EMBL:AAZ87223.1, RC ECO:0000313,Proteomes:UP000002529}; RX PubMed=16275786; DOI=10.1093/nar/gki954; RA Yang F., Yang J., Zhang X., Chen L., Jiang Y., Yan Y., Tang X., RA Wang J., Xiong Z., Dong J., Xue Y., Zhu Y., Xu X., Sun L., Chen S., RA Nie H., Peng J., Xu J., Wang Y., ...
http://pbil.univ-lyon1.fr/cgi-bin/acnuc-search-ac?query=Q3Z4T9&db=SWISSPROT
Correlated protein conformational states and membrane dynamics during attack by pore-forming toxins ePrints@IIScCorrelated protein conformational states and membrane dynamics during attack by pore-forming toxins [email protected]
Pore-forming toxins (PFTs) are a class of proteins implicated in a wide range of virulent bacterial infections and diseases. These toxins bind to target membranes and subsequently oligomerize to form functional pores that eventually lead to cell lysis. While the protein undergoes large conformational changes on the bilayer, the connection between intermediate oligomeric states and lipid reorganization during pore formation is largely unexplored. Cholesterol-dependent cytolysins (CDCs) are a subclass of PFTs widely implicated in food poisoning and other related infections. Using a prototypical CDC, listeriolysin O (LLO), we provide a microscopic connection between pore formation, lipid dynamics, and leakage kinetics by using a combination of Forster resonance energy transfer (FRET) and fluorescence correlation spectroscopy (FCS) measurements on single giant unilamellar vesicles (GUVs). Upon exposure to LLO, two distinct populations of GUVs with widely different leakage kinetics emerge. We ...
http://eprints.iisc.ac.in/63227/
Bacillus thuringiensis | Caudata.org: Newts and Salamanders PortalBacillus thuringiensis | Caudata.org: Newts and Salamanders Portal
I have been interested in using Bacillus thuringiensis in an outdoor amphibian setup to kill mosquito larvae. Mosquitoes have become more than a nuisance...
https://www.caudata.org/threads/bacillus-thuringiensis.47326/
bacillus thuringiensis Archives - Ideas Galorebacillus thuringiensis Archives - Ideas Galore
Bacillus thuringiensis (Bt) eggplant or bt talong in Philippine dialect was genetically redesigned to be resistant to the fruit and shoot borer larva that affects crop yielding from 50-70 percent.. Read moreScientist Warns About Risk Of Bt Eggplant. ...
https://affleap.com/tag/bacillus-thuringiensis/
CIL:41753, Bacillus thuringiensis. CIL. DatasetCIL:41753, Bacillus thuringiensis. CIL. Dataset
Negative staining image of Bacillus thuringiensis, which produces spores and crystals in times of stress. The image was taken using transmission elec...
http://ccdb.ucsd.edu/images/41753
CIL:41756, Bacillus thuringiensis. CIL. Dataset
Negative staining image of Bacillus thuringiensis, which produces spores and crystals in times of stress. The image was taken using transmission elec...
http://www.cellimagelibrary.org/images/41756
100 Years of Bacillus thuringiensis: A Critical Scientific Assessment | ASM.org100 Years of Bacillus thuringiensis: A Critical Scientific Assessment | ASM.org
This report from the Academy describes the use of Bacillus thuringiensis proteins in agriculture and addresses positive outcomes, potential risks and recommendations for future research.
https://asm.org/Reports/100-Years-of-Bacillus-thuringiensis-A-Critical-Sci
Bacillus thuringiensis HD-73 spores have surface-localized Cry1Ac toxin: Physiological and pathogenic consequences -...
Fingerprint Dive into the research topics of Bacillus thuringiensis HD-73 spores have surface-localized Cry1Ac toxin: Physiological and pathogenic consequences. Together they form a unique fingerprint. ...
https://experts.nebraska.edu/en/publications/bacillus-thuringiensis-hd-73-spores-have-surface-localized-cry1ac/fingerprints/?sortBy=alphabetically
Record details
The cry1Ac gene produces the insect control protein Cry1Ac, a delta-endotoxin, in the plant tissues. Cry proteins, of which Cry1Ac is only one, act by selectively binding to specific sites localized on the lining of the midgut of susceptible insect species. Following binding, pores are formed that disrupt midgut ion flow, causing gut paralysis and eventual death due to bacterial sepsis. Cry1Ac is insecticidal only when eaten by the larvae of lepidopteran insects (moths and butterflies), and its specificity of action is directly attributable to the presence of specific binding sites in the target insects. There are no binding sites for delta-endotoxins of B. thuringiensis on the surface of mammalian intestinal cells, therefore, livestock animals and humans are not susceptible to these proteins ...
http://bioseguridadecuador.gob.ec/database/record.shtml?documentid=14938
Insecticidal protein elisa and antibodyInsecticidal protein elisa and antibody
Shop Insecticidal protein ELISA Kit, Recombinant Protein and Insecticidal protein Antibody at MyBioSource. Custom ELISA Kit, Recombinant Protein and Antibody are available.
https://www.mybiosource.com/protein_family.php?root=insecticidal-protein
Haemolysin expression modulating protein familyHaemolysin expression modulating protein family
... the haemolysin expression modulating protein family is a family of proteins. This family consists of haemolysin expression ... "Evidence for direct protein-protein interaction between members of the enterobacterial Hha/YmoA and H-NS families of proteins ... The HHA family of proteins display striking similarity to the oligomerisation domain of the H-NS proteins. Madrid C, Nieto JM, ... These proteins act as modulators of bacterial gene expression. Members of this family act in conjunction with members of the H- ...
https://en.wikipedia.org/wiki/Haemolysin_expression_modulating_protein_family
HemolysinHemolysin
However, hemolysins are often capable of lysing red blood cells in vitro. While most hemolysins are protein compounds, some are ... Hemolysins or haemolysins are lipids and proteins that cause lysis of red blood cells by disrupting the cell membrane. Although ... γ-Hemolysins are pore-forming toxins in the same family as α-Hemolysin. They are unique in that they come in two components, ... But hemolysin is related to bacteria not only in this way but also in some others. As mentioned before, hemolysin is a ...
https://en.wikipedia.org/wiki/Hemolysin
Haemolysin EHaemolysin E
... (HlyE) is a protein family that consists of several enterobacterial haemolysin (HlyE) proteins. Hemolysin E (HlyE ... HlyE is unrelated to the well characterised pore-forming E. coli hemolysins of the RTX family, haemolysin A. HlyE is a protein ... Wallace AJ, Stillman TJ, Atkins A, Jamieson SJ, Bullough PA, Green J, Artymiuk PJ (January 2000). "E. coli hemolysin E (HlyE, ... This article incorporates text from the public domain Pfam and InterPro: IPR013057 (Protein domains, Bacterial toxins). ...
https://en.wikipedia.org/wiki/Haemolysin_E
CytolysinCytolysin
... s comprise more than 1/3 of all bacterial protein toxins. Bacterial protein toxins can be highly poisonous to human. ... are named hemolysins, and so on. Cytolysins may be involved in immunity as well as in venoms. Hemolysin is also used by certain ... For this reason "Hemolysin" was first used to describe any MDTs. In the 1960s certain MDTs were proved to be destructive on ... Most receptors are proteins, but they can be other molecules as well, such as lipids or sugars. With the help of receptors, ...
https://en.wikipedia.org/wiki/Cytolysin
Staphylococcus aureus delta toxinStaphylococcus aureus delta toxin
Universal protein resource accession number P0C1V1 for "Delta-hemolysin" at UniProt. Dinges MM, Orwin PM, Schlievert PM ( ... v t e (Protein pages needing a picture, Bacterial toxins, All stub articles, Biochemistry stubs). ... Delta toxin molecules activate a G-protein-coupled receptor expressed in leukocytes called formyl-peptide receptor 2 (FPR2), ... Thelestam M, Möllby R, Wadström T (December 1973). "Effects of staphylococcal alpha-, beta-, delta-, and gamma-hemolysins on ...
https://en.wikipedia.org/wiki/Staphylococcus_aureus_delta_toxin
NanoporeNanopore
Examples of pore-forming proteins are alpha hemolysin, aerolysin, and MspA porin. In typical laboratory nanopore experiments, a ... Nanopores may be formed by pore-forming proteins, typically a hollow core passing through a mushroom-shaped protein molecule. ... Newer pore-forming proteins have been extracted from bacteriophages for study into their use as nanopores. These pores are ... It may, for example, be created by a pore-forming protein or as a hole in synthetic materials such as silicon or graphene. When ...
https://en.wikipedia.org/wiki/Nanopore
Peripheral membrane proteinPeripheral membrane protein
Such transformations occur in pore forming toxins such as colicin A, alpha-hemolysin, and others. They may also occur in BcL-2 ... Some proteins, such as G-proteins and certain protein kinases, interact with transmembrane proteins and the lipid bilayer ... Sterol carrier proteins Phosphatidylinositol transfer proteins and STAR domains Oxysterol-binding protein These proteins are ... Peripheral membrane proteins, or extrinsic membrane proteins, are membrane proteins that adhere only temporarily to the ...
https://en.wikipedia.org/wiki/Peripheral_membrane_protein
Adenylate cyclase toxinAdenylate cyclase toxin
The hemolysin portion of the protein then binds to the target membrane and inserts itself into the bilayer. The adenylate ... Conjugating each subdomain to a different protein allows protein-protein interactions to be studied, because cAMP production ... Adenylate cyclase toxin from Bordetella pertussis is a 1706 amino acid residue long protein. The protein consists of three ... are a characteristic feature of this family of proteins, and are able to bind calcium ions. A feature of the RTX proteins is ...
https://en.wikipedia.org/wiki/Adenylate_cyclase_toxin
RTX toxinRTX toxin
The cyaA operon encodes the five proteins CyaA (RTX toxin), CyaC (CyaA activation protein), and the three T1SS proteins: CyaB ( ... EHEC haemolysin (EHEC-Hly) was discovered in the EHEC serotype O157:H7. The EHEC-Hly operon contains four E. coli hly homologs ... an ABC transporter) CyaD (a membrane fusion protein), and CyaE (an outer membrane protein). The CyaA protein contains an ... The general rtx gene cluster encodes three protein types: the RTX toxin, an RTX activating acyltransferase, and T1SS proteins. ...
https://en.wikipedia.org/wiki/RTX_toxin
PleurotolysinPleurotolysin
Berne S, Krizaj I, Pohleven F, Turk T, Macek P, Sepcić K (April 2002). "Pleurotus and Agrocybe hemolysins, new proteins ... Representative proteins include pleurotolysin B, which has a MACPF domain, the aegerolysin-like protein pleurotolysin A, and ... Another two-component hemolysin, erylysin A and B (EryA and EryB; TC# 1.C.97.1.2), was isolated from an edible mushroom, ... Proteins with membrane-attack complex/perforin (MACPF) domains have a variety of biological roles, including defense and attack ...
https://en.wikipedia.org/wiki/Pleurotolysin
LeptospirosisLeptospirosis
It can also recruit complement regulators such as Factor H, C4b-binding protein, factor H-like binding protein, and vitronectin ... Leptospira also secretes sphingomyelinase and haemolysin that target red blood cells. Leptospira spreads rapidly to all organs ... ERU is an autoimmune disease involving antibodies against Leptospira proteins LruA and LruB cross-reacting with eye proteins. ... They also bind to several human proteins such as complement proteins, thrombin, fibrinogen, and plasminogen using surface ...
https://en.wikipedia.org/wiki/Leptospirosis
Cry6AaCry6Aa
The protein has structural similarities to other toxins, including haemolysin E and B. cereus toxins HlbB and NheA. No other ... Since Cry6Aa proteins function differently than other Cry proteins, they are combined with other proteins to decrease the ... Most Cry proteins have 3 main domains with functional homology across proteins, domain I contains an alpha helix bundle, domain ... Mutations in required proteins for necrosis inhibit Cry6Aa, but not other Cry proteins, revealing a rare mechanism in Cry6Aa. ...
https://en.wikipedia.org/wiki/Cry6Aa
Staphylococcus pseudintermediusStaphylococcus pseudintermedius
Protein A, an immunoglobulin binding protein, has been found on the surface of S. pseudintermedius. Protein A attaches to the ... The pore-forming cytotoxins, α-hemolysin and β-hemolysin, lyse erythrocytes of sheep and rabbits. Leukotoxin destroys host ... The previously mentioned protein A as well as clumping factor are surface proteins that allow the bacteria to bind to host ... S. pseudintermedius has been found to produce biofilms, an extracellular matrix of protein, DNA, and polysaccharide, which aids ...
https://en.wikipedia.org/wiki/Staphylococcus_pseudintermedius
TetanospasminTetanospasmin
The tetanus toxin protein has a molecular weight of 150 kDa. It is translated from the tetX gene as one protein which is ... C. tetani also produces the exotoxin tetanolysin, a hemolysin, that causes destruction of tissues. Tetanus toxin spreads ... Both the ganglioside and the GPI-anchored protein are located in lipid microdomains and both are requisite for specific TeNT ... The A-chain, an M27-family zinc endopeptidase, attacks the vesicle-associated membrane protein (VAMP). The TetX gene encoding ...
https://en.wikipedia.org/wiki/Tetanospasmin
Contact-dependent growth inhibitionContact-dependent growth inhibition
September 2013). "Haemolysin coregulated protein is an exported receptor and chaperone of type VI secretion substrates". ... The first protein encoded in the operon, CdiB, is an outer membrane beta-barrel protein that exports CdiA, presenting it on the ... This effector kills targets that do not have the cognate immunity protein similar to other CDI systems. The first CDI system to ... CdiI is an immunity protein to prevent auto-inhibition by the C-terminal toxin. This also prevents the bacteria from killing or ...
https://en.wikipedia.org/wiki/Contact-dependent_growth_inhibition
Type VI secretion systemType VI secretion system
... arranged as a sheath around a tube built from stacked hexameric rings of the haemolysin co-regulated protein (Hcp). At the tip ... The immunity proteins function by binding to the toxin proteins, often at their active site, thereby blocking their activity. ... "Haemolysin coregulated protein is an exported receptor and chaperone of type VI secretion substrates". Molecular Cell. 51 (5): ... Upon the GacS/Rsm pathway stimulation, an increase in Rsm molecules leads to inhibition of mRNA-binding protein RsmA. RsmA is a ...
https://en.wikipedia.org/wiki/Type_VI_secretion_system
Virulence factorVirulence factor
One example of a bacterial virulence factor acting like a eukaryotic protein is Salmonella protein SopE it acts as a GEF, ... The factors, including toxins, hemolysins and proteases, bring damage to the host. Bacteria produce various adhesins including ... YopT (Yersinia outer protein T) from Yersinia is an example of modification of the host. It modifies the proteolytic cleavage ... One is by acting as a GEF or GAP, and proceeding to look like a normally eukaryotic cellular protein. The other is covalently ...
https://en.wikipedia.org/wiki/Virulence_factor
Pathogenicity islandPathogenicity island
1990). "Deletions of chro- mosomal regions coding for fimbriae and hemolysins occur in vivo and in vitro in various ... Regulation genes typically encoded on PAIs include AraC-like proteins and two-component response regulators. PAIs can be ... The P fimbriae island contains virulence factors such as haemolysin, pili, cytotoxic necrosing factor, and uropathogenic ... specific protein (USP). Yersinia pestis high pathogenicity island I has genes regulating iron uptake and storage. Salmonella ...
https://en.wikipedia.org/wiki/Pathogenicity_island
Group A streptococcal infectionGroup A streptococcal infection
Several virulence factors contribute to the pathogenesis of GAS, such as M protein, hemolysins, and extracellular enzymes. ... The 30-valent N-terminal M-protein-based vaccine as well as the M-protein vaccine (minimal epitope J8 vaccine) are two vaccines ... The M-protein generates antibodies that cross-react with autoantigens on interstitial connective tissue, in particular of the ... A 2019 study shows that GAS's evasion of immune detection is facilitated by protein S, an extracellular and cell wall- ...
https://en.wikipedia.org/wiki/Group_A_streptococcal_infection
Pore-forming toxinPore-forming toxin
... conformation as seen in α-Haemolysin. (PDB: 7AHL, 1T5R) β-PFTs are dimorphic proteins that exist as soluble monomers and then ... Figure 1 shows the pore-form of α-Hemolysin, the first crystal structure of a β-PFT in its pore-form. 7 α-Hemolysin monomers ... Figure 1 shows the pore-form of α-Hemolysin, the first crystal structure of a β-PFT in its pore-form. 7 α-Hemolysin monomers ... As discussed above, the majority of the Toxin_10 family proteins act as part of binary toxins with partner proteins that may ...
https://en.wikipedia.org/wiki/Pore-forming_toxin
ExotoxinExotoxin
The effector proteins injected by the type III secretion apparatus of Yersinia into target cells are one example. Another group ... The prototype member of the RTX toxin family is haemolysin A (HlyA) of E. coli.[citation needed] RTX is also found in ... Superantigens bridge the MHC class II protein on antigen-presenting cells with the T-cell receptor on the surface of T cells ... Membrane-damaging toxins exhibit hemolysin or cytolysin activity in vitro. However, induction of cell lysis may not be the ...
https://en.wikipedia.org/wiki/Exotoxin
Treponema pallidumTreponema pallidum
Sequencing has revealed a bundle of twelve proteins and some putative hemolysins are potential virulence factors of T. pallidum ... They are composed of the intermediate filament-like protein CfpA (cytoplasmic filament protein A). Although the filaments may ... The outer membrane of T. pallidum has too few surface proteins for an antibody to be effective. Efforts to develop a safe and ... Treponemal outer membrane proteins are key factors for its pathogenesis, persistence and immune evasion strategies. TP0326 is ...
https://en.wikipedia.org/wiki/Treponema_pallidum
Nanopore sequencingNanopore sequencing
Alpha hemolysin (αHL), a nanopore from bacteria that causes lysis of red blood cells, has been studied for over 15 years. To ... Protein mutation of αHL has improved the detection abilities of the pore. The next proposed step is to bind an exonuclease onto ... Imagine now a nano-sized polymer such as DNA or protein placed in one of the chambers. This molecule also has a net charge that ... In 1989 he sketched out a plan to drive a single-strand of DNA through a protein nanopore embedded into a thin membrane as part ...
https://en.wikipedia.org/wiki/Nanopore_sequencing
ExoenzymeExoenzyme
Hemolysins target erythrocytes, a.k.a. red blood cells. Attacking and lysing these cells harms the host organism, and provides ... Once active, pepsin works to break down proteins in foods such as dairy, meat, and eggs. Pepsin works best at the pH of gastric ... This enzyme is responsible for the breakdown of large globular proteins and its activity is specific to cleaving the C-terminal ... Bacteria such as Clostridium do so by using the enzyme to dissolve collagen and hyaluronic acid, the protein and saccharides, ...
https://en.wikipedia.org/wiki/Exoenzyme
Bacillus haemolytic enterotoxinBacillus haemolytic enterotoxin
This article incorporates text from the public domain Pfam and InterPro: IPR008414 (Protein pages needing a picture, Protein ... Haemolysin BL and non-haemolytic enterotoxin production are both influenced by pH and micro. Phelps RJ, McKillip JL (June 2002 ... Haemolysin BL (encoded by HBL) and non-haemolytic enterotoxin (encoded by NHE), represent the major enterotoxins produced by ... In molecular biology, the Bacillus haemolytic enterotoxin family of proteins consists of several Bacillus haemolytic ...
https://en.wikipedia.org/wiki/Bacillus_haemolytic_enterotoxin
Vibrio vulnificusVibrio vulnificus
The capsular proteins the bacteria express however, are capable of producing an immune response contributing to shock syndrome ... Exotoxin: V. vulnificus produces a number of extracellular toxins such as metalloprotease VvpE, cytolysin/hemolysin VvhA, and ...
https://en.wikipedia.org/wiki/Vibrio_vulnificus
Colin Hughes (microbiologist)Colin Hughes (microbiologist)
Science 266:1992-1996 [8] Thanabalu et al (2000) Substrate-induced assembly of a contiguous channel for protein export from E. ... Fatty acylation of two internal lysine residues required for the toxic activity of Escherichia coli hemolysin. ... Nature 504:287-290 [13] Koronakis et al (2000) Crystal structure of ToIC central to multidrug efflux and protein export. Nature ... Docking of chaperone-substrate complexes at the membrane ATPase during flagellar type III protein export. Proc Natl Acad Sci ( ...
https://en.wikipedia.org/wiki/Colin_Hughes_(microbiologist)
Membrane proteinMembrane protein
Polypeptide toxins and many antibacterial peptides, such as colicins or hemolysins, and certain proteins involved in apoptosis ... Membrane proteins, like soluble globular proteins, fibrous proteins, and disordered proteins, are common. It is estimated that ... Membrane proteins are common, and medically important-about a third of all human proteins are membrane proteins, and these are ... Membrane proteins are common proteins that are part of, or interact with, biological membranes. Membrane proteins fall into ...
https://en.wikipedia.org/wiki/Membrane_protein
Francisella tularensisFrancisella tularensis
This protein is located in a putative pathogenicity island regulated by the transcription factor MglA. F. tularensis, in vitro ... A hemolysin activity, named NlyA, with immunological reactivity to Escherichia coli anti-HlyA antibody, was identified in ... Acid phosphatase AcpA has been found in other bacteria to act as a hemolysin, whereas in Francisella, its role as a virulence ... It also contains a number of ATP-binding cassette (ABC) proteins that may be linked to the secretion of virulence factors. F. ...
https://en.wikipedia.org/wiki/Francisella_tularensis
Antibody-dependent cellular cytotoxicityAntibody-dependent cellular cytotoxicity
Antibodies can then bind to these viral proteins. Next, the NK cells which have reciprocal Fcγ receptors will bind to that ... described as hemolysins. These bacteria target the CD18 portion of leukocytes, which has historically been shown to impact ADCC ... During replication of a virus, some of the viral proteins are expressed on the cell surface membrane of the infected cell. ... more recent studies have produced success in regulating metastatic tumors using interleukin proteins to activate the NK cell. ...
https://en.wikipedia.org/wiki/Antibody-dependent_cellular_cytotoxicity
Rocky Mountain spotted feverRocky Mountain spotted fever
This species of Rickettsia uses an abundant cell surface protein called OmpB to attach to a host cell membrane protein called ... To escape from the phagosome, the bacteria secrete phospholipase D and hemolysin C. This causes disruption of the phagosomal ... CDC42, protein tyrosine kinase, phosphoinositide 3-kinase, and Src-family kinases then activate Arp2/3. This causes the ... Both rOmpA and rOmpB are members of a family of surface cell antigens (Sca) which are autotransporter proteins; they act as ...
https://en.wikipedia.org/wiki/Rocky_Mountain_spotted_fever
HemolysisHemolysis
Hemolysins damage the red blood cell's cytoplasmic membrane, causing lysis and eventually cell death. From hemo- + -lysis, from ... The release of heme leads to the production of bilirubin and depletion of plasma proteins, such as albumin, haptoglobin, and ... One cause of hemolysis is the action of hemolysins, toxins that are produced by certain pathogenic bacteria or fungi. Another ... Hemolysin Glucose-6-phosphate dehydrogenase deficiency Wells, John C. (2008). Longman Pronunciation Dictionary (3rd ed.). ...
https://en.wikipedia.org/wiki/Hemolysis
Beta-propeller phytaseBeta-propeller phytase
β-propeller phytases (BPPs) are a group of enzymes (i.e. protein superfamily) with a round beta-propeller structure. BPPs are ... "The effects of calcium and other polyvalent cations on channel formation by Escherichia coli alpha-hemolysin in red blood cells ... Gifre L, Arís A, Bach À, Garcia-Fruitós E (March 2017). "Trends in recombinant protein use in animal production". Microbial ... Chen C, Cheng K, Ko T, Guo R (2015-04-01). "Current Progresses in Phytase Research: Three-Dimensional Structure and Protein ...
https://en.wikipedia.org/wiki/Beta-propeller_phytase
List of MeSH codes (D27)List of MeSH codes (D27)
... protein kinase inhibitors MeSH D27.505.519.389.760 - protein synthesis inhibitors MeSH D27.505.519.389.936 - uncoupling agents ... hemolysins MeSH D27.888.569.213.545 - leukocidins MeSH D27.888.569.242 - dermotoxins MeSH D27.888.569.271 - immunotoxins MeSH ...
https://en.wikipedia.org/wiki/List_of_MeSH_codes_(D27)
Kausik ChattopadhyayKausik Chattopadhyay
He is known for his studies on the Pore-forming protein toxins and T-cell costimulatory molecules. The Department of ... "Disulphide bond restrains the C-terminal region of thermostable direct hemolysin during folding to promote oligomerization". ... Kausik Chattopadhyay is an Indian structural biologist, protein biologist, and a professor at the Department of Biological ...
https://en.wikipedia.org/wiki/Kausik_Chattopadhyay
Septic shockSeptic shock
Recombinant activated protein C (drotrecogin alpha) in a 2011 Cochrane review was found not to decrease mortality and to ... Type II, membrane-damaging toxins, destroy cell membranes in order to enter and include hemolysins and phospholipases. Type III ... Martí-Carvajal AJ, Solà I, Gluud C, Lathyris D, Cardona AF (December 2012). "Human recombinant protein C for severe sepsis and ... This is manifested by increased cellular respiration, protein catabolism, and metabolic acidosis with a compensatory ...
https://en.wikipedia.org/wiki/Septic_shock
Clostridium novyiClostridium novyi
The toxin is a large 250-kDa protein the active part of which is the NH2-terminal 551 amino acid fragment. Alpha-toxins are ... The zeta-toxin is characterised as haemolysin.[citation needed] The type and severity of the disease caused depends on ... Just I, Selzer J, Wilm M, von Eichel-Streiber C, Mann M, Aktories K (June 1995). "Glucosylation of Rho proteins by Clostridium ... October 1996). "Clostridium novyi alpha-toxin-catalyzed incorporation of GlcNAc into Rho subfamily proteins". The Journal of ...
https://en.wikipedia.org/wiki/Clostridium_novyi
List of MeSH codes (D12.776)List of MeSH codes (D12.776)
... groel protein MeSH D12.776.602.500.500.100 - fusion proteins, bcr-abl MeSH D12.776.602.500.500.320 - fusion proteins, gag-onc ... hemolysins MeSH D12.776.377.715.548.114.573 - immune sera MeSH D12.776.377.715.548.114.573.203 - antilymphocyte serum MeSH ... oncogene protein v-maf MeSH D12.776.964.700.750.875 - oncogene proteins v-abl MeSH D12.776.964.700.750.882 - oncogene proteins ... fusion proteins, gag-pol MeSH D12.776.964.775.350.400 - hiv core protein p24 MeSH D12.776.964.775.375.325 - fusion proteins, ...
https://en.wikipedia.org/wiki/List_of_MeSH_codes_(D12.776)
Staphylococcus aureus alpha toxinStaphylococcus aureus alpha toxin
Alpha-hemolysin has been used extensively in academic research as a single molecule nanopore sensor. In 1996 it was first shown ... The structure of the protein has been solved by x-ray crystallography and is deposited in the PDB as id code 7ahl. Seven ... The hly gene on the S. aureus chromosome encodes the 293 residue protein monomer, which forms heptameric units on the cellular ... Song L, Hobaugh MR, Shustak C, Cheley S, Bayley H, Gouaux JE (December 1996). "Structure of staphylococcal alpha-hemolysin, a ...
https://en.wikipedia.org/wiki/Staphylococcus_aureus_alpha_toxin
Bordetella pertussisBordetella pertussis
The bacterium contains a surface protein, filamentous haemagglutinin adhesin, which binds to the sulfatides found on cilia of ... Sebo, Peter; Osicka, Radim; Masin, Jiri (2014-08-04). "Adenylate cyclase toxin-hemolysin relevance for pertussis vaccines". ... The toxin, known as pertussis toxin (or PTx), inhibits G protein coupling that regulates an adenylate cyclase-mediated ...
https://en.wikipedia.org/wiki/Bordetella_pertussis
Synthetic ion channelsSynthetic ion channels
While modification of large protein channels using mutagenesis are generally considered out of the scope of synthetic channels ... the demarcation is not sharp, as supramolecular or covalent bonding of cyclodextrins to alpha-hemolysin demonstrates. An ion ... Nanotechnology Supramolecular chemistry Macrocycles Amphiphile Ionophore Membrane biophysics Membrane protein Voltage-gated ion ... "Molecular bases of cyclodextrin adapter interactions with engineered protein nanopores". PNAS. 107 (18): 8165-8170. Bibcode: ...
https://en.wikipedia.org/wiki/Synthetic_ion_channels
Vibrio parahaemolyticusVibrio parahaemolyticus
... the thermostable direct hemolysin gene (tdh) and/or the tdh-related hemolysin gene (trh). Strains possessing one or both of ... Aside from the T3SS, two genes encoding well-characterized virulence proteins are typically found on the PAI, ... which is capable of injecting virulence proteins into host cells to disrupt host cell functions or cause cell death by ... these hemolysins exhibit beta-hemolysis on blood agar plates. A distinct correlation seems to exist between presence of tdh, ...
https://en.wikipedia.org/wiki/Vibrio_parahaemolyticus
List of MeSH codes (D12.776.124)List of MeSH codes (D12.776.124)
... complement c1 inactivator proteins MeSH D12.776.124.486.274.920.250.500 - complement c1 inhibitor protein MeSH D12.776.124.486. ... hemolysins MeSH D12.776.124.486.485.114.573 - immune sera MeSH D12.776.124.486.485.114.573.203 - antilymphocyte serum MeSH ... bence jones protein MeSH D12.776.124.486.485.900.225 - cryoglobulins MeSH D12.776.124.486.485.900.500 - myeloma proteins MeSH ... bence jones protein MeSH D12.776.124.790.651.900.225 - cryoglobulins MeSH D12.776.124.790.651.900.500 - myeloma proteins MeSH ...
https://en.wikipedia.org/wiki/List_of_MeSH_codes_(D12.776.124)
Pathogenic Escherichia coliPathogenic Escherichia coli
Tauschek M, Gorrell R, Robins-Browne RM (2002). "Identification of a protein secretory pathway for the secretion of heat-labile ... Uropathogenic E. coli produce alpha- and beta-hemolysins, which cause lysis of urinary tract cells.[citation needed] Another ...
https://en.wikipedia.org/wiki/Pathogenic_Escherichia_coli
L-form bacteriaL-form bacteria
Here, the absence of a cell wall can allow production of large amounts of secreted proteins that would otherwise accumulate in ... Sieben S, Hertle R, Gumpert J, Braun V (October 1998). "The Serratia marcescens hemolysin is secreted but not activated by ... These strains are being examined for possible uses in biotechnology as host strains for recombinant protein production. ... Choi JH, Lee SY (June 2004). "Secretory and extracellular production of recombinant proteins using Escherichia coli". Appl. ...
https://en.wikipedia.org/wiki/L-form_bacteria
ATP-binding cassette transporterATP-binding cassette transporter
... a membrane fusion protein (MFP), and an outer membrane factor (OMF). An example is the secretion of hemolysin (HlyA) from E. ... roles of membrane structure and electrostatics in lipid-protein and protein-protein interactions" (PDF). Biochimica et ... A "2 TMS" protein has 2 transmembrane segments) to give 6 TMS proteins. ABC2 exporters evolved by intragenic duplication of a 3 ... The genome of the model plant Arabidopsis thaliana is capable of encoding 120 ABC proteins compared to 50-70 ABC proteins that ...
https://en.wikipedia.org/wiki/ATP-binding_cassette_transporter
Aeromonas hydrophilaAeromonas hydrophila
T3SS is a specialized protein secretion machinery that exports virulence factors directly to host cells. These factors subvert ... hemolysins, and enterotoxins. However, the pathogenic mechanisms are unknown. The recently proposed type-III secretion system ( ... understanding of them The pathogenicity of Aeromonas species was believed to be mediated by a number of extracellular proteins ...
https://en.wikipedia.org/wiki/Aeromonas_hydrophila
CyaACyaA
The RTX hemolysin part of CyaA is functionally independent of AC domain and forms oligomeric cation-selective pores that allow ... In monocytes and macrophages, the CyaA-produced cAMP signaling through the cAMP/protein kinase A (PKA) pathway blocks the ... Sebo P, Osicka R, Masin J (October 2014). "Adenylate cyclase toxin-hemolysin relevance for pertussis vaccines". Expert Review ... such as protein kinase A, are clustered. Inside the cells, the AC enzyme binds calmodulin and catalyzes unregulated conversion ...
https://en.wikipedia.org/wiki/CyaA
HCPHCP
Haemolysin-coregulated protein, in the Type VI secretion system Healthcare proxy Aga Khan Historic Cities Programme Habitat ...
https://en.wikipedia.org/wiki/HCP
Lecithinase CLecithinase C
Protein Structure. 351 (1): 155-71. doi:10.1016/0005-2795(74)90074-9. PMID 4365891. Phospholipase+C at the US National Library ... heat-labile hemolysin, α-toxin) is an enzyme with systematic name phosphatidylcholine cholinephosphohydrolase. This enzyme ... phosphocholine The bacterial enzyme is a zinc protein. It also acts on sphingomyelin and phosphatidylinositol. Druzhinina KV, ...
https://en.wikipedia.org/wiki/Lecithinase_C
Cynthia BurrowsCynthia Burrows
To examine the extent of damage in G-quadruplexes of telomeres, Burrows used a protein α-hemolysin, which contains a nanoscale ... Xu, Xiaoyun; Muller, James G.; Ye, Yu; Burrows, Cynthia J. (2008-01-16). "DNA-protein cross-links between guanine and lysine ... In the context of DNA-protein cross linking, 8-oxoguanine is susceptible to forming adducts with amino acids containing ... Nanopores can range from solid-state constructs to small proteins. ...
https://en.wikipedia.org/wiki/Cynthia_Burrows
Stichodactyla helianthusStichodactyla helianthus
"Purification And Characterization Of Two Hemolysins From Stichodactyla Helianthus". Toxicon, vol 39, no. 2-3, 2001, pp. 187-194 ... Protein Expression And Purification, vol 123, 2016, pp. 42-50. (Articles with short description, Short description is different ...
https://en.wikipedia.org/wiki/Stichodactyla_helianthus
Paroxysmal nocturnal hemoglobinuria (PNH): MedlinePlus Medical EncyclopediaParoxysmal nocturnal hemoglobinuria (PNH): MedlinePlus Medical Encyclopedia
Flow cytometry to measure certain proteins. *Ham (acid hemolysin) test. *Serum hemoglobin and haptoglobin ... Without PIG-A, important proteins cannot connect to the cell surface and protect the cell from substances in the blood called ... to help certain proteins stick to cells. ...
https://medlineplus.gov/ency/article/000534.htm
Single DNA rotaxanes of a transmembrane pore proteinSingle DNA rotaxanes of a transmembrane pore protein
An α-hemolysin transmembrane pore can be threaded in a desired orientation by DNA-PEG hybrid strands to yield functional ... An α-hemolysin transmembrane pore can be threaded in a desired orientation by DNA-PEG hybrid strands to yield functional ... Single DNA rotaxanes of a transmembrane pore protein Angew Chem Int Ed Engl. 2004 Jun 7;43(23):3063-7. doi: 10.1002/anie. ...
https://pubmed.ncbi.nlm.nih.gov/15188482/
Plants | Free Full-Text | Nematicidal Activity of the Endophyte Serratia ureilytica against Nacobbus aberrans in Chili Plants ...Plants | Free Full-Text | Nematicidal Activity of the Endophyte Serratia ureilytica against Nacobbus aberrans in Chili Plants ...
Genes related to the synthesis of chitinases, siderophores, proteases C, serralisins, hemolysin, and serrawettin W2 that have ... Hemolysin activation protein. 7930-9444. Serralysin. Publishers Note: MDPI stays neutral with regard to jurisdictional claims ... The mean GC content is 59.3%, similar to S. marcescens strains; 954 hypothetical proteins and 4394 proteins with assigned ... Serratia ureilytica UTS Protein. 1-6213. 87709-93531. 3-2393. 1-408. 3-206. Putative peptide synthetase, containing non- ...
https://www.mdpi.com/2223-7747/10/12/2655
DeCS 2007 - Changed termsDeCS 2007 - Changed terms
Hemolysins. Hemolysin Proteins. Lymphotoxin. Lymphotoxin-alpha. Monocyte Chemoattractant Protein-1. Chemokine CCL2. ...
https://decs.bvs.br/I/rep_i2007.htm
Recombinant Mouse EGF-containing fibulin-like extracellular matrix protein 1 (Efemp1) | CSB-MP007450MO | CusabioRecombinant Mouse EGF-containing fibulin-like extracellular matrix protein 1 (Efemp1) | CSB-MP007450MO | Cusabio
Recombinant Mouse EGF-containing fibulin-like extracellular matrix protein 1 (Efemp1) from Cusabio. Cat Number: CSB-MP007450MO ... Recombinant Staphylococcus aureus Gamma-hemolysin component C (hlgC) , CSB-EP751944SKYa0 Cusabio Staphylococcus aureus ... Human Extracellular matrix protein 1 (ECM1) ELISA kit , CSB-EL007383HU , CusabioHuman Extracellular matrix protein 1 (ECM1) ... Recombinant Mouse EGF-containing fibulin-like extracellular matrix protein 1 (Efemp1) , CSB-MP007450MO. (No reviews yet) Write ...
https://joplink.net/recombinant-mouse-egf-containing-fibulin-like-extracellular-matrix-protein-1-efemp1-csb-mp007450mo/
Acute Pyelonephritis: Practice Essentials, Background, PathophysiologyAcute Pyelonephritis: Practice Essentials, Background, Pathophysiology
Pseudomonas aeruginosa has several mechanisms that promote adherence, including alginate, other membrane proteins, pili, and ... the inhibition of attachment of type 1 fimbriae E coli to uroepithelial cells by tubular cell-secreted Tamm-Horsfall protein, ...
https://www.medscape.com/answers/245559-19888/what-is-the-role-of-urinalysis-in-the-diagnosis-of-acute-pyelonephritis-kidney-infection
Frontiers | Trichosanthes pericarpium Aqueous Extract Enhances the Mobilization of Endothelial Progenitor Cells and Up...Frontiers | Trichosanthes pericarpium Aqueous Extract Enhances the Mobilization of Endothelial Progenitor Cells and Up...
In brief, the total protein concentration was determined by the BCA protein Assay Kit. Protein was separated by 10% reduced ... Then hemolysin was dissolved in distilled water, and applied to cells at the final concentration 10% (v/v). The CD34+/vWF+ ... Total tissue protein was extracted using the total protein extraction kit mentioned above according to manufacturers ... Then hemolysin was dissolved in distilled water, and applied to cells at the final concentration 10% (v/v). (A) The forward- ...
https://www.frontiersin.org/articles/10.3389/fphys.2017.01132/full
Protein identification by nanopore peptide profiling | Nature CommunicationsProtein identification by nanopore peptide profiling | Nature Communications
Using model proteins, we show that the spectra resulting from nanopore experiments and mass spectrometry share similar profiles ... Here, we show that an engineered Fragaceatoxin C nanopore is capable of identifying individual proteins by measuring peptide ... Our work shows the potential of a low-cost, portable nanopore-based analyzer for protein identification. Peptide mass ... hence allowing protein fingerprinting. The intensity of individual peaks provides information on the concentration of ...
https://www.nature.com/articles/s41467-021-26046-9?error=cookies_not_supported&code=d936bf42-18da-4dc2-9b6b-15de08eabea6
Team:UNICAMP-Brazil/Coliguard/Killing - 2009.igem.orgTeam:UNICAMP-Brazil/Coliguard/Killing - 2009.igem.org
is the hemolysin itself and . HlyC. codifies to a protein important to make . HlyA. active (1). ... The best system we found is the alpha hemolysin secretion system. The alpha hemolysin secretions system is encoded in a operon ... HlyA is the hemolysin itself and HlyC codifies to a protein important to make HlyA active (1). ... HlyA is the hemolysin itself and HlyC codifies to a protein important to make HlyA active (1). ...
https://2009.igem.org/wiki/index.php?title=Team:UNICAMP-Brazil/Coliguard/Killing&diff=cur&oldid=161633
JKU-FoDok Forschungsdokumentation der Universit t Linz - Publikation - Contributions of the Hydrophobic Helix 2 of the...
"Contributions of the Hydrophobic Helix 2 of the Bordetella pertussis CyaA-hemolysin to Membrane Permeabilization" , in Protein ... Contributions of the Hydrophobic Helix 2 of the Bordetella pertussis CyaA-hemolysin to Membrane Permeabilization ...
https://fodok.uni-linz.ac.at/fodok/publikation.xsql?PUB_ID=63501
Artificial cell design: reconstructing biology for life science applications | Emerging Topics in Life Sciences | Portland PressArtificial cell design: reconstructing biology for life science applications | Emerging Topics in Life Sciences | Portland Press
Incorporation of membrane proteins by insertion (e.g. α-hemolysin) or by reconstitution (e.g. ATP synthase) allows passage of ... In vitro protein expression systems, also known as cell-free protein expression systems (CFPS), are often found to have ... CF protein expression systems have a huge impact on this process where therapeutic proteins are encoded within artificial cells ... Photosynthetic reaction centre proteins (RC) are transmembrane proteins embedded in the photosynthetic cell membrane ...
https://portlandpress.com/emergtoplifesci/article/doi/10.1042/ETLS20220050/232121/Artificial-cell-design-reconstructing-biology-for?searchresult=1
Klauda LabKlauda Lab
Ion channel proteins are widely used in biology. Shown here is the alpha-hemolysin protein that a bateria uses to help invade a ... This protein has also been used in biotech applicaitons to measure macromolecules like sequencing DNA. The movie shows how ions ... In this example the lipid packing sensor of the Osh4 protein (ALPS-peptide) is shown to bind to the membrane. MD simulations ...
https://terpconnect.umd.edu/~jbklauda/movies.html
TCDB » SuperfamiliesTCDB » Superfamilies
Protein Kinase (PK) Superfamily. Protein kinase domains are sometimes found in transport proteins. These include TC#s 1.A.87.2. ... 1.C.3 - The α-Hemolysin Channel-forming Toxin (αHL) Family. 1.C.4 - The Aerolysin Channel-forming Toxin (Aerolysin) Family. 1.C ... GTP-Binding Protein/GTPase (GTP-BP) Superfamily. The GTP-Binding Protein/GTPase Superfamily includes proteins from many multi- ... These proteins usually have 4 - 6 TMSs and function in intracellular protein trafficking in eukaryotes and as protein chaparone ...
https://tcdb.org/superfamily.php
NIOSHTIC-2 Search Results - Full View
Fungal hemolysins are potential virulence factors. Some fungal hemolysins belong to the aegerolysin protein family that ... The recombinant protein was purified and using MALDI-TOF MS determined to have a mass of 16,428 Da. Circular dichroism analysis ... Fungi; Proteins; Hemolysis; Genes; Recombinant-DNA; Antibody-response; Temperature-effects; Thermal-reactions; Molds; Lung- ... Here, we describe a hemolysin from Aspergillus terreus called terrelysin. We used the genome sequence database to identify the ...
http://www2a.cdc.gov/nioshtic-2/BuildQyr.asp?s1=DNA+or+Deoxyribonucleic+acid&f1=TI&Startyear=&t1=1&s2=DNA+or+Deoxyribonucleic+acid&terms=3&Adv=1&ct=&B1=Search&f2=KW&Limit=500&Sort=DP+DESC&D1=10&EndYear=&PageNo=23&RecNo=221&View=f&
Oxford Researchers Demonstrate Feasibility of Protein Nanopores for RNA Base Recognition | GenomeWebOxford Researchers Demonstrate Feasibility of Protein Nanopores for RNA Base Recognition | GenomeWeb
Alpha-hemolysin, the pore-producing protein used to read immobilized RNA bases in the current study, has already been the ... Hence, it will be necessary to combine [alpha-hemolysin or an alternative protein nanopore with a processive RNA translocating ... The proof-of-principle study demonstrated that modified alpha-hemolysin proteins with slightly larger than wild type pore ... The University of Oxford laboratory behind the alpha-hemolysin protein-based nanopore DNA sequencing strategy being developed ...
https://www.genomeweb.com/sequencing/oxford-researchers-demonstrate-feasibility-protein-nanopores-rna-base-recognitio
Complete Sequence and Molecular Epidemiology of IncK Epidemic Plasmid Encoding blaCTX-M-14 - Volume 17, Number 4-April 2011 -...Complete Sequence and Molecular Epidemiology of IncK Epidemic Plasmid Encoding blaCTX-M-14 - Volume 17, Number 4-April 2011 -...
... and a hemolysin transport protein. pO113 was isolated from another hemolysin-producing EHEC O113:H21 E. coli sample from a ... Other weak protein matches show some homology to the extracytoplasmic function sigma factors, small regulatory proteins ... Annotation of the plasmid showed 115 potential protein CDSs, 89 of which were homologous to proteins of known function ( ... Open reading frames are color coded as follows: brown, pseudogenes; orange, hypothetic proteins; light pink, insertion ...
http://www.cdc.gov/eid/content/17/4/645.htm
Difference between revisions of "User:GillenK" - microbewiki
In contrast to the IM Sec proteins through which proteins pass in an unfolded state, proteins may partially fold in the ... hemolysins. ] exotoxins that lyse host cells to release nutrients and pili that mediate attachment to substrates. In gram- ... In contrast to the IM Sec proteins through which proteins pass in an unfolded state, proteins may partially fold in the ... In contrast to the IM Sec proteins through which proteins pass in an unfolded state, proteins may partially fold in the ...
https://microbewiki.kenyon.edu/index.php?title=User:GillenK&diff=prev&oldid=57151
Volume 59 (2020): Issue 1 (January 2020)Volume 59 (2020): Issue 1 (January 2020)
α-hemolysin HlyA. 3.2. Cytotoxic necrotizing factor 1. 3.3. Toxin Vat. 4. Protein Usp. 5. Protein Ag43. 6. Bacterial biofilm. 7 ... α-hemolysin HlyA. 3.2. Cytotoxic necrotizing factor 1. 3.3. Toxin Vat. 4. Protein Usp. 5. Protein Ag43. 6. Bacterial biofilm. 7 ... Furthermore, the presence of protein Ag43 enhances adhesion of UPEC within the urinary tract, aggregation and biofilm formation ... Furthermore, the presence of protein Ag43 enhances adhesion of UPEC within the urinary tract, aggregation and biofilm formation ...
https://sciendo.com/it/issue/AM/59/1
World Journal of Gastroenterology - Baishideng Publishing GroupWorld Journal of Gastroenterology - Baishideng Publishing Group
Bioengineering of LAB vector expressing Haemolysin co-regulated protein (Hcp): a strategic approach to control gut colonization ...
https://www.wjgnet.com/1007-9327/CitedArticlesInF6?id=10.3390%2Fmicroorganisms8040482
Researcher Studies Common Bacteria to Give Babies a Fighting Chance - On the PulseResearcher Studies Common Bacteria to Give Babies a Fighting Chance - On the Pulse
Most bacteria that infect humans produce toxins known as hemolysins, which typically are proteins. What we found with GBS is ... that it is not using a protein toxin to kill host cells, as previously believed, but its using different cell component known ...
https://pulse.seattlechildrens.org/researcher-studies-common-bacteria-to-give-babies-a-fighting-chance/
Jellyfish Venom Proteins May Inhibit Growth of Breast Cancer Cells | Universitas Gadjah MadaJellyfish Venom Proteins May Inhibit Growth of Breast Cancer Cells | Universitas Gadjah Mada
Research by a mix of Biology and Pharmacy undergraduates has found that jellyfish venom proteins have the potential to become ... They have various peptides, enzymes, neurotoxins, cytolysins, and hemolysins, which function as an antimicrobial, anti- ... Jellyfish Venom Proteins May Inhibit Growth of Breast Cancer Cells. Jellyfish Venom Proteins May Inhibit Growth of Breast ... In this study, the jellyfish venom proteins were tethered with ER-α and observed through in silico modeling. The protein ...
https://ugm.ac.id/en/news/22040-jellyfish-venom-proteins-may-inhibit-growth-of-breast-cancer-cells
HOMD :: SEQF2888HOMD :: SEQF2888
channel protein%2C hemolysin III family. 33. SEQF2888,AENP01000032.1. EFR32172.1 jb [NA] [AA] 210/69. 63912-64121. conserved ... ribosomal protein S9. 187. SEQF2888,AENP01000028.1. EFR32326.1 jb [NA] [AA] 447/148. 24773-24327. DNA repair protein RadC ... conserved hypothetical protein. 111. SEQF2888,AENP01000030.1. EFR32250.1 jb [NA] [AA] 927/308. 23983-23057. TIM-barrel protein% ... replication initiator protein A%2C N-terminal domain protein. 151. SEQF2888,AENP01000030.1. EFR32290.1 jb [NA] [AA] 1287/428. ...
https://homd.org/genome/explorer?gid=SEQF2888&anno=ncbi
CIPSM - DNA Origami Gatekeepers for Solid-State Nanopores
Biological nanopores, such as alpha-hemolysin, can be customized within the limits of protein engineering. Artificial nanopores ...
https://cipsm.de/publications/research-area-a/dna-origami-gatekeepers-for-solid-state-nanopores/
N,N,N-Trimethyl-2-(phosphonooxy)ethanaminium: Uses, Interactions, Mechanism of Action | DrugBank OnlineN,N,N-Trimethyl-2-(phosphonooxy)ethanaminium: Uses, Interactions, Mechanism of Action | DrugBank Online
UEpididymal sperm-binding protein 1. Not Available. Humans. UGamma-hemolysin component B. Not Available. Staphylococcus aureus ... Details3. Gamma-hemolysin component B. Kind. Protein. Organism. Staphylococcus aureus (strain MW2). Pharmacological action. ... Berman HM, Westbrook J, Feng Z, Gilliland G, Bhat TN, Weissig H, Shindyalov IN, Bourne PE: The Protein Data Bank. Nucleic Acids ... IndicationContraindications & Blackbox WarningsPharmacodynamicsMechanism of actionAbsorptionVolume of distributionProtein ...
https://go.drugbank.com/drugs/DB03945
Belfast School of Architecture & the Be - Research output - Ulster UniversityBelfast School of Architecture & the Be - Research output - Ulster University
IMSEAR at SEARO: Browsing DSpace
Browsing by Author Hemolysin Proteins --ultrastructure Jump to: 0-9 A B C D E F G H I J K L M N O P Q R S T U V W X Y Z or ... Hemolysin Proteins --ultrastructure; Mazumdar, Budhaditya; Ganguly, Sreerupa; Ghosh, Amar N; Banerjee, Kalyan K. ... The role of C-terminus carbohydrate-binding domain of Vibrio cholerae haemolysin/cytolysin in the conversion of the pre-pore β- ...
https://imsear.searo.who.int/browse?type=author&sort_by=1&order=ASC&rpp=20&etal=-1&value=Hemolysin+Proteins+--ultrastructure&starts_with=B
Phenotypic and Genetic Diversity of Uropathogenic Enterococcus faecalis Strains Isolated in the Primorsky Region of Russia |...Phenotypic and Genetic Diversity of Uropathogenic Enterococcus faecalis Strains Isolated in the Primorsky Region of Russia |...
... hemolysin, gelatinase, enterococcal surface protein, aggregation substance, serine protease, capsule, etc.). The greatest ... coding Eep protein, which conduces to formation of a biofilm, making it resistant to various biological stress factors) were ... Enterococcal surface protein Esp is important for biofilm formation of Enterococcus faecium. E1162. Journal of Bacteriology. ... surface protein Esp in the pathogenesis of ascending urinary tract infection. Infection and Immunity. 2001;. 69. (7):4366-4372 ...
https://www.intechopen.com/chapters/63199
Target identification in Fusobacterium nucleatum by subtractive genomics approach and enrichment analysis of host-pathogen...Target identification in Fusobacterium nucleatum by subtractive genomics approach and enrichment analysis of host-pathogen...
... through subtractive genomics approach and host-pathogen protein-protein interactions (HP-PPIs). We also carried out enrichment ... Hence, proteins from IBDsite, a database for inflammatory bowel disease (IBD) research and those involved in colorectal ... These target proteins might be considered for designing potent inhibitors to abrogate F. nucleatum infections. From enrichment ... Subtractive genomics analysis provided a set of target proteins suggested to be indispensable for survival and pathogenicity of ...
https://bmcmicrobiol.biomedcentral.com/articles/10.1186/s12866-016-0700-0
Alpha-hemolysinPeptidesEscherichiaSecretionStaphylococcusVirulenceRecombinant proteinMembrane proteinExotoxinsColiMoleculesHumansBacillusSingle-moleculePore proteinToxinLipidBacterial proteinsCharacterizationExtracellularCytolysinsComplexesSubstancesNanoporeFungiReceptorsProteasePurificationBiosynthesis proteinHlyBBiologySolubleCytoplasmicSilicoBindsPolyclonalIntracellularLigandsAnomalousTargetsInducesRegulatoryHigh-throughputMembranesSuperfamilyBiologicalCellContributesCrystal ProteinCells
Alpha-hemolysin10
  • The best system we found is the alpha hemolysin secretion system. (igem.org)
  • The alpha hemolysin secretions system is encoded in a operon containing four genes, Picture1A: hlyD and HlyB constitutes the transporter, HlyA is the hemolysin itself and HlyC codifies to a protein important to make HlyA active (1). (igem.org)
  • Shown here is the alpha-hemolysin protein that a bateria uses to help invade a host. (umd.edu)
  • The University of Oxford laboratory behind the alpha-hemolysin protein-based nanopore DNA sequencing strategy being developed by Oxford Nanopores Technologies has shown that it's possible to use similar nanopores for recognizing RNA bases. (genomeweb.com)
  • The proof-of-principle study demonstrated that modified alpha-hemolysin proteins with slightly larger than wild type pore diameters produced distinguishable signals for all four RNA bases, as well as three modified bases. (genomeweb.com)
  • To that end, she is currently looking at enzyme-based options for processively sequencing RNA molecules as they move through the alpha-hemolysin pore. (genomeweb.com)
  • Alpha-hemolysin, the pore-producing protein used to read immobilized RNA bases in the current study, has already been the subject of much development for DNA sequencing. (genomeweb.com)
  • In particular, Oxford Nanopore has been developing nanopore sequencing devices based on alpha-hemolysin research by Bayley, the company's founder and a member of its board of directors. (genomeweb.com)
  • For the new study, Ayub and Bayley used RNA oligonucleotides tagged with biotin to characterize the current profiles that each RNA base would produce if it were to pass through alpha-hemolysin pores in a sequencing setting. (genomeweb.com)
  • Biological nanopores, such as alpha-hemolysin, can be customized within the limits of protein engineering. (cipsm.de)
Peptides6
  • Peptides are of special interest for protein characterization, as they allow identification analogous to bottom-up MS-based proteomics. (nature.com)
  • The resulting collected peptides describe a spectrum that can then be used to identify proteins (Fig. 1 ). (nature.com)
  • We used a motif-based strategy to screen selected proteins of M. tuberculosis for peptides predicted to bind to human leukocyte antigen (HLA)-A*0201. (unthsc.edu)
  • These peptides were derived from the 38-kDa antigen and the 28-kDa hemolysin, the latter being a novel target for CD8+ T cells. (unthsc.edu)
  • The chemical nature and precise position of posttranslational modifications (PTMs) in proteins or peptides are crucial for various severe diseases, such as cancer. (nanion.de)
  • Here, we demonstrate the use of a protein nanopore to differentiate peptides─derived from human histone H4 protein─of identical mass according to the positions of acetylated and methylated lysine residues. (nanion.de)
Escherichia5
  • This page will summarize how proteins are targeted to various compartments in Gram-negative cells, taking most examples from the well-studied Escherichia coli ( E. coli ). (kenyon.edu)
  • During the past two decades, disease caused by Escherichia intimin protein ( eaeA ), and hemolysin ( hly ) (9). (cdc.gov)
  • protein_coding" "AAC73596","tesA","Escherichia coli","acyl-CoA thioesterase 1 and protease I and lysophospholipase L1 [Ensembl]. (ntu.edu.sg)
  • protein_coding" "AAC76562","bcsF","Escherichia coli","DUF2636 family cellulose production small membrane protein [Ensembl]. (ntu.edu.sg)
  • protein_coding" "AAC76745","bglF","Escherichia coli","fused beta-glucoside-specific PTS enzymes: IIA component/IIB component/IIC component [Ensembl]. (ntu.edu.sg)
Secretion7
  • E. coli, as a Gram negative bacteria, doesn't have a well developed secretion system to transport proteins to the extracellular medium. (igem.org)
  • These ATPase are found as coomponents of several protein secretion systems as well as synaptosomal fusion systems. (tcdb.org)
  • In contrast to the IM Sec proteins through which proteins pass in an unfolded state, proteins may partially fold in the periplasm prior to secretion through the OM. (kenyon.edu)
  • Purification of recombinant proteins in E. coli is aided by targeting the proteins to particular cell locations and studies of bacterial toxin secretion may lead to novel therapeutic agents. (kenyon.edu)
  • Here, we studied the interaction of environmental V. cholerae with eukaryotic predators or competing bacteria and tested the contributions of the hemolysin and the type VI secretion system (T6SS) to those interactions. (biorxiv.org)
  • We focused on the type VI secretion system (T6SS) and the pore forming toxin hemolysin, which are tightly regulated in pandemic strains but remain constitutively active in non-pandemic isolates. (biorxiv.org)
  • Secretion monitor precursor protein (SecM) [Interproscan]. (ntu.edu.sg)
Staphylococcus2
  • Staphylococcal α-hemolysin (α-HL) is a β-barrel pore-forming toxin (PFT) expressed by Staphylococcus aureus. (elsevier.com)
  • Purified, His-tagged Staphylococcus aureus Gamma Hemolysin C (Hlg C) recombinant protein. (multxpert.com)
Virulence3
  • Fungal hemolysins are potential virulence factors. (cdc.gov)
  • In subtractive genomics approach, the essential, virulence and resistance related proteins were retrieved from RefSeq proteome of F. nucleatum by searching against Database of Essential Genes (DEG), Virulence Factor Database (VFDB) and Antibiotic Resistance Gene-ANNOTation (ARG-ANNOT) tool respectively. (biomedcentral.com)
  • Hemolysin II (HlyII) is one of the virulence factors of the opportunistic bacterium Bacillus cereus belonging to the group of ß-pore-forming toxins. (bvsalud.org)
Recombinant protein1
  • The recombinant protein was purified and using MALDI-TOF MS determined to have a mass of 16,428 Da. (cdc.gov)
Membrane protein1
  • Shotgun proteomic analysis of total and membrane protein extracts of S. aureus strain N315. (genebiosystems.com)
Exotoxins2
  • Examples include hemolysins exotoxins that lyse host cells to release nutrients and pili that mediate attachment to substrates. (kenyon.edu)
  • Bacterial multiplication and the production of soluble proteins called exotoxins require a low oxygen tension. (medscape.com)
Coli1
  • Targeting of proteins to different cellular compartments in E. coli. (kenyon.edu)
Molecules2
  • Proteins and other molecules - collectively known as cargos - can enter and exit the nucleus via tiny channels in the membrane that surrounds and protects it. (elifesciences.org)
  • Further studies from an optical interferometric profiler have shown that both rosette and scalloped protein patterns are hierarchical structures of concentric rings consisting of many prism-like columnar stacks, with each of the stack having thousands of protein molecules. (epj.org)
Humans1
  • Most bacteria that infect humans produce toxins known as hemolysins, which typically are proteins. (seattlechildrens.org)
Bacillus1
  • Region Met225 to Ile412 of Bacillus cereus Hemolysin II Is Capable to Agglutinate Red Blood Cells. (bvsalud.org)
Single-molecule1
  • Nanopores are single-molecule sensors used in nucleic acid analysis, whereas their applicability towards full protein identification has yet to be demonstrated. (nature.com)
Pore protein1
  • On that front, researchers suspect it may be possible to use an enzyme to help ratchet single-stranded RNA through a protein nanopore, similar to the approach that the University of Washington's Jens Gundlach and University of California, Santa Cruz, researcher Mark Akeson have described for DNA sequencing with the MspA pore protein ( IS 3/27/2012 ). (genomeweb.com)
Toxin1
  • What we found with GBS is that it is not using a protein toxin to kill host cells, as previously believed, but it's using different cell component known as a lipid. (seattlechildrens.org)
Lipid2
  • In this example the lipid packing sensor of the Osh4 protein (ALPS-peptide) is shown to bind to the membrane. (umd.edu)
  • Phosphoinositide-incorporated lipid-protein Nanodiscs: A tool for studying protein-lipid interactions. (hokudai.ac.jp)
Bacterial proteins1
  • Prediction of HP-PPIs exhibited 186 interactions contributed by 103 host and 76 bacterial proteins. (biomedcentral.com)
Characterization2
  • Phylogenetic characterization of transport protein superfamilies: superiority of SuperfamilyTree programs over those based on multiple alignments. (tcdb.org)
  • Characterization of recombinant terrelysin, a hemolysin of Aspergillus terreus. (cdc.gov)
Extracellular2
  • In addition to targeting to various cellular locations, some proteins are destined to leave the cell entirely and enter the extracellular environment. (kenyon.edu)
  • In addition to the cytoplasm, Gram-negative bacteria must selectively localize proteins to the inner membrane (IM), periplasm, outer membrane (OM), and extracellular environment (Figure 1). (kenyon.edu)
Cytolysins1
  • Some fungal hemolysins belong to the aegerolysin protein family that includes cytolysins capable of lysing erythrocytes and other cells. (cdc.gov)
Complexes1
  • PCS-based structure determination of protein-protein complexes. (hokudai.ac.jp)
Substances1
  • Without PIG-A, important proteins cannot connect to the cell surface and protect the cell from substances in the blood called complement. (medlineplus.gov)
Nanopore6
  • Here, we show that an engineered Fragaceatoxin C nanopore is capable of identifying individual proteins by measuring peptide spectra that are produced from hydrolyzed proteins. (nature.com)
  • Using model proteins, we show that the spectra resulting from nanopore experiments and mass spectrometry share similar profiles, hence allowing protein fingerprinting. (nature.com)
  • Our work shows the potential of a low-cost, portable nanopore-based analyzer for protein identification. (nature.com)
  • The nanopore approach might then provide a low-cost and high-throughput approach to protein identification. (nature.com)
  • Fig. 1: Graphical overview of the nanopore protein fingerprinting approach. (nature.com)
  • Nanopore-Based Protein Identification. (univ-evry.fr)
Fungi1
  • Proteins from BACTERIA and FUNGI that are soluble enough to be secreted to target ERYTHROCYTES and insert into the membrane to form beta-barrel pores. (bvsalud.org)
Receptors2
  • Receptor proteins - called nuclear transport receptors - bind to potential cargos and shuttle them through the channels. (elifesciences.org)
  • This selective transport process relies on the nuclear transport receptors being attracted to flexible, spaghetti-like proteins that are anchored to the walls on the inside of each channel. (elifesciences.org)
Protease2
  • In this contribution, we show that G13F-FraC-T1 can be used to directly sample proteins that are digested by a protease. (nature.com)
  • In some examples, the proteins include a prostate-specific protease cleavage site and can further include a prostate-tissue-specific binding domain which functionally replaces the native PA binding domain. (justia.com)
Purification1
  • In addition, separation of detergent-soluble parasite extracts using strong cation-exchange chromatography, resulted in purification of 60-65 kDa proteins with trypsin-sensitive haemolytic activity. (dundee.ac.uk)
Biosynthesis protein1
  • Cellulose biosynthesis protein BcsF [Interproscan]. (ntu.edu.sg)
HlyB1
  • To use the hemolysin system we intend to construct a biobrick with HlyB and HlyD and 252 bp of the carboxy terminal region of HlyA, using primers in Silver Standard, Picture 1B. (igem.org)
Biology2
  • Ion channel proteins are widely used in biology. (umd.edu)
  • Research by a mix of Biology and Pharmacy undergraduates has found that jellyfish venom proteins have the potential to become an inhibitor of breast cancer cells. (ugm.ac.id)
Soluble2
  • α-HL is secreted as a water-soluble monomeric protein, which binds to target membranes and forms membrane-inserted heptameric pores. (elsevier.com)
  • A soluble form of HlyIILCTD was obtained using the SlyD chaperone protein . (bvsalud.org)
Cytoplasmic2
  • While it has been long assumed that prokaryotes lack the sophistical intracellular architecture of eukaryotic cells, more recent research has shown that prokaryotes do indeed have cytoplasmic organization with various proteins restricted to certain areas. (kenyon.edu)
  • Description: Host cell factor 2(HCFC2) is a cytoplasmic protein. (gentaurtop.com)
Silico2
  • In this study, the jellyfish venom proteins were tethered with ER-α and observed through in silico modeling. (ugm.ac.id)
  • By combining these in silico predictions with experimental validations, we showed that highly similar but nonidentical immunity proteins were insufficient to provide cross-immunity among those wild strains. (biorxiv.org)
Binds1
  • Polyglutamine tract-binding protein-1 binds to U5-15kD via a continuous 23-residue segment of the C-terminal domain. (hokudai.ac.jp)
Polyclonal1
  • Results from thermal denaturation of rTerrelysin show that the protein maintained the beta-sheet confirmation up to 65 degrees C. Polyclonal antibody to rTerrelysin recognized a protein of approximately 16.5 kDa in mycelial extracts from A. terreus. (cdc.gov)
Intracellular1
  • We speculate that hemolysins may alter the phagosomal membrane surrounding intracellular M. tuberculosis, allowing themselves and other antigens to gain access to the major histocompatibility complex class I processing pathway. (unthsc.edu)
Ligands1
  • CBS domains are found attached to a wide range of other protein domains suggesting that CBS domains may play a regulatory role making proteins sensitive to adenosyl carrying ligands. (embl.de)
Anomalous1
  • Semi-automated protein crystal mounting device for the sulfur single-wavelength anomalous diffraction method. (hokudai.ac.jp)
Targets3
  • Protein sorting to the various organelles in eukaryotic cells is widely studied and resulted in a Nobel Prize to Blobel for the recognition of the signal sequence that initially targets secreted and membrane proteins to the endoplasmic reticulum. (kenyon.edu)
  • A subsequent hierarchical screening to identify non-human homologous, metabolic pathway-independent/pathway-specific and druggable proteins resulted in eight pathway-independent and 27 pathway-specific druggable targets. (biomedcentral.com)
  • The evidence indicates that trivalent, methylated, and relatively less ionizable arsenic metabolites may be capable of interacting with cellular targets such as proteins and even DNA [Kitchin 2001]. (cdc.gov)
Induces1
  • 2-Methyl-2,4-pentanediol induces spontaneous assembly of staphylococcal α-hemolysin into heptameric pore structure. (hokudai.ac.jp)
Regulatory1
  • Bacterial regulatory proteins [Interproscan]. (ntu.edu.sg)
High-throughput1
  • This molecular shape-sensing principle offers a path to versatile, label-free, and high-throughput characterizations of protein isoforms. (nanion.de)
Membranes1
  • In gram-negative cells secreted proteins must cross both the inner and outer membranes. (kenyon.edu)
Superfamily2
  • The ABC2 uptake Superfamily includes proteins in TC families 2.A.87 (P-RFT) and 2.A.88 (VUT or ECF). (tcdb.org)
  • The A-OMP superfamily consists of Actinobacterial proteins of about 500 aas with ~8 alpha helical TMSs. (tcdb.org)
Biological1
  • Beyond to contributing to basic biological research, studies of protein sorting in bacteria have biotechnology and medical applications (Cormelius, P., 2000). (kenyon.edu)
Cell3
  • Thanassim and Hultgren (2000) have reviewed the different pathways proteins may take on their voyage outside the Gram-negative cell. (kenyon.edu)
  • Mis-localized proteins are non-functional and can lead to cell death. (kenyon.edu)
  • Marginal filaments, where efficacious and beneficial venom proteins are found, are believed by several previous studies to have content that can inhibit cell migration," said Khintan. (ugm.ac.id)
Contributes1
  • This surface localized protein contributes isolates of 99.2% tested positive. (cdc.gov)
Crystal Protein1
  • In an attempt to increase the insecticidal effect of the δ-endotoxin crystal protein CryIC on the relatively Cry-insensitive larvae of Spodoptera littoralis, a combination of CryIC and endochitinase was used. (agri.gov.il)
Cells5
  • People with this disease have blood cells that are missing a gene called PIG-A. This gene allows a substance called glycosyl-phosphatidylinositol (GPI) to help certain proteins stick to cells. (medlineplus.gov)
  • Sorting proteins to their correct cellular location is of critical importance to cells. (kenyon.edu)
  • A closely migrating doublet of hookworm proteins with apparent molecular weights of 60-65 kDa bound to the erythrocyte membrane after lysis of cells using both unlabeled and biotinylated detergent-solubilised hookworm extracts. (dundee.ac.uk)
  • Hemolysin II Is Capable to Agglutinate Red Blood Cells. (bvsalud.org)
  • Expression of non-structural-1A binding protein in lung epithelial cells is modulated by miRNA-548an on exposure to influenza A virus. (cdc.gov)
Paroxysmal nocturnal hemoglobinuria (PNH): MedlinePlus Medical Encyclopedia
Paroxysmal nocturnal hemoglobinuria (PNH): MedlinePlus Medical Encyclopedia (medlineplus.gov)
Acute Pyelonephritis: Practice Essentials, Background, Pathophysiology
Acute Pyelonephritis: Practice Essentials, Background, Pathophysiology (medscape.com)
Frontiers | Trichosanthes pericarpium Aqueous Extract Enhances the Mobilization of Endothelial Progenitor Cells and Up...
Frontiers | Trichosanthes pericarpium Aqueous Extract Enhances the Mobilization of Endothelial Progenitor Cells and Up... (frontiersin.org)
Protein identification by nanopore peptide profiling | Nature Communications
Protein identification by nanopore peptide profiling | Nature Communications (nature.com)
Frontiers | Functional Characterization and Conditional Regulation of the Type VI Secretion System in Vibrio fluvialis
Frontiers | Functional Characterization and Conditional Regulation of the Type VI Secretion System in Vibrio fluvialis (frontiersin.org)
Urinary Tract Infection (UTI) and Cystitis (Bladder Infection) in Females: Practice Essentials, Background, Pathophysiology
Urinary Tract Infection (UTI) and Cystitis (Bladder Infection) in Females: Practice Essentials, Background, Pathophysiology (medscape.com)
Recombinant Mouse EGF-containing fibulin-like extracellular matrix protein 1 (Efemp1) | CSB-MP007450MO | Cusabio
Recombinant Mouse EGF-containing fibulin-like extracellular matrix protein 1 (Efemp1) | CSB-MP007450MO | Cusabio (joplink.net)
Difference between revisions of User:GillenK - microbewiki
Difference between revisions of "User:GillenK" - microbewiki (microbewiki.kenyon.edu)
Shiga Toxin-producing Escherichia coli Strains Negative for Locus of Enterocyte Effacement - Volume 15, Number 3-March 2009 -...
Shiga Toxin-producing Escherichia coli Strains Negative for Locus of Enterocyte Effacement - Volume 15, Number 3-March 2009 -... (wwwnc.cdc.gov)
Streptococcus suis Sequence Type 7 Outbreak, Sichuan, China - Volume 12, Number 8-August 2006 - Emerging Infectious Diseases...
Streptococcus suis Sequence Type 7 Outbreak, Sichuan, China - Volume 12, Number 8-August 2006 - Emerging Infectious Diseases... (wwwnc.cdc.gov)
Isolation of Escherichia coli O157:H7 from Intact Colon Fecal Samples of Swine - Volume 9, Number 3-March 2003 - Emerging...
Isolation of Escherichia coli O157:H7 from Intact Colon Fecal Samples of Swine - Volume 9, Number 3-March 2003 - Emerging... (wwwnc.cdc.gov)
Volume 59 (2020): Issue 1 (January 2020)
Volume 59 (2020): Issue 1 (January 2020) (sciendo.com)
World Journal of Gastroenterology - Baishideng Publishing Group
World Journal of Gastroenterology - Baishideng Publishing Group (wjgnet.com)
Diversity | Free Full-Text | Genome Sequence of Dickeya solani, a New soft Rot Pathogen of Potato, Suggests its Emergence May...
Diversity | Free Full-Text | Genome Sequence of Dickeya solani, a New soft Rot Pathogen of Potato, Suggests its Emergence May... (mdpi.com)
Researcher Studies Common Bacteria to Give Babies a Fighting Chance - On the Pulse
Researcher Studies Common Bacteria to Give Babies a Fighting Chance - On the Pulse (pulse.seattlechildrens.org)
HOMD :: SEQF2888
HOMD :: SEQF2888 (homd.org)
Detecting protein analytes that modulate transmembrane movement of a polymer chain within a single protein pore | Nature...
Detecting protein analytes that modulate transmembrane movement of a polymer chain within a single protein pore | Nature... (nature.com)
CIPSM - DNA Origami Gatekeepers for Solid-State Nanopores
CIPSM - DNA Origami Gatekeepers for Solid-State Nanopores (cipsm.de)
N,N,N-Trimethyl-2-(phosphonooxy)ethanaminium: Uses, Interactions, Mechanism of Action | DrugBank Online
N,N,N-Trimethyl-2-(phosphonooxy)ethanaminium: Uses, Interactions, Mechanism of Action | DrugBank Online (go.drugbank.com)
Cold Agglutinin Disease: Practice Essentials, Pathophysiology, Etiology
Cold Agglutinin Disease: Practice Essentials, Pathophysiology, Etiology (medscape.com)
Belfast School of Architecture & the Be - Research output - Ulster University
Belfast School of Architecture & the Be - Research output - Ulster University (pure.ulster.ac.uk)
Complete nucleotide sequence of pRS218, a large virulence plasmid, that augments pathogenic potential of meningitis-associated...
Complete nucleotide sequence of pRS218, a large virulence plasmid, that augments pathogenic potential of meningitis-associated... (bmcmicrobiol.biomedcentral.com)
Department of Physics - Research output - Experts@Syracuse
Department of Physics - Research output - [email protected] (experts.syr.edu)
Autoimmune theory of disease (MPKB)
Autoimmune theory of disease (MPKB) (mpkb.org)
A physical model describing the interaction of nuclear transport receptors with FG nucleoporin domain assemblies | eLife
A physical model describing the interaction of nuclear transport receptors with FG nucleoporin domain assemblies | eLife (elifesciences.org)
Staphylococcus | Concise Medical Knowledge
Staphylococcus | Concise Medical Knowledge (lecturio.com)
Network Portal - Gene DVU2093
Network Portal - Gene DVU2093 (networks.systemsbiology.net)