Mesenchymal Stem Cell Transplantation: Transfer of MESENCHYMAL STEM CELLS between individuals within the same species (TRANSPLANTATION, HOMOLOGOUS) or transfer within the same individual (TRANSPLANTATION, AUTOLOGOUS).Mesenchymal Stromal Cells: Bone-marrow-derived, non-hematopoietic cells that support HEMATOPOETIC STEM CELLS. They have also been isolated from other organs and tissues such as UMBILICAL CORD BLOOD, umbilical vein subendothelium, and WHARTON JELLY. These cells are considered to be a source of multipotent stem cells because they include subpopulations of mesenchymal stem cells.Hematopoietic Stem Cell Transplantation: Transfer of HEMATOPOIETIC STEM CELLS from BONE MARROW or BLOOD between individuals within the same species (TRANSPLANTATION, HOMOLOGOUS) or transfer within the same individual (TRANSPLANTATION, AUTOLOGOUS). Hematopoietic stem cell transplantation has been used as an alternative to BONE MARROW TRANSPLANTATION in the treatment of a variety of neoplasms.Stem Cell Transplantation: The transfer of STEM CELLS from one individual to another within the same species (TRANSPLANTATION, HOMOLOGOUS) or between species (XENOTRANSPLANTATION), or transfer within the same individual (TRANSPLANTATION, AUTOLOGOUS). The source and location of the stem cells determines their potency or pluripotency to differentiate into various cell types.Transplantation, Homologous: Transplantation between individuals of the same species. Usually refers to genetically disparate individuals in contradistinction to isogeneic transplantation for genetically identical individuals.Stem Cells: Relatively undifferentiated cells that retain the ability to divide and proliferate throughout postnatal life to provide progenitor cells that can differentiate into specialized cells.Transplantation, Autologous: Transplantation of an individual's own tissue from one site to another site.Disease Models, Animal: Naturally occurring or experimentally induced animal diseases with pathological processes sufficiently similar to those of human diseases. They are used as study models for human diseases.Rats, Sprague-Dawley: A strain of albino rat used widely for experimental purposes because of its calmness and ease of handling. It was developed by the Sprague-Dawley Animal Company.Transplantation Conditioning: Preparative treatment of transplant recipient with various conditioning regimens including radiation, immune sera, chemotherapy, and/or immunosuppressive agents, prior to transplantation. Transplantation conditioning is very common before bone marrow transplantation.Peripheral Blood Stem Cell Transplantation: Transplantation of stem cells collected from the peripheral blood. It is a less invasive alternative to direct marrow harvesting of hematopoietic stem cells. Enrichment of stem cells in peripheral blood can be achieved by inducing mobilization of stem cells from the BONE MARROW.Graft vs Host Disease: The clinical entity characterized by anorexia, diarrhea, loss of hair, leukopenia, thrombocytopenia, growth retardation, and eventual death brought about by the GRAFT VS HOST REACTION.Myocardial Infarction: NECROSIS of the MYOCARDIUM caused by an obstruction of the blood supply to the heart (CORONARY CIRCULATION).Hematopoietic Stem Cells: Progenitor cells from which all blood cells derive.Cell Differentiation: Progressive restriction of the developmental potential and increasing specialization of function that leads to the formation of specialized cells, tissues, and organs.Bone Marrow Cells: Cells contained in the bone marrow including fat cells (see ADIPOCYTES); STROMAL CELLS; MEGAKARYOCYTES; and the immediate precursors of most blood cells.Bone Marrow Transplantation: The transference of BONE MARROW from one human or animal to another for a variety of purposes including HEMATOPOIETIC STEM CELL TRANSPLANTATION or MESENCHYMAL STEM CELL TRANSPLANTATION.Hematologic Neoplasms: Neoplasms located in the blood and blood-forming tissue (the bone marrow and lymphatic tissue). The commonest forms are the various types of LEUKEMIA, of LYMPHOMA, and of the progressive, life-threatening forms of the MYELODYSPLASTIC SYNDROMES.Liver Transplantation: The transference of a part of or an entire liver from one human or animal to another.Cord Blood Stem Cell Transplantation: Transplantation of STEM CELLS collected from the fetal blood remaining in the UMBILICAL CORD and the PLACENTA after delivery. Included are the HEMATOPOIETIC STEM CELLS.Cells, Cultured: Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others.Transplantation Chimera: An organism that, as a result of transplantation of donor tissue or cells, consists of two or more cell lines descended from at least two zygotes. This state may result in the induction of donor-specific TRANSPLANTATION TOLERANCE.Adult Stem Cells: Cells with high proliferative and self renewal capacities derived from adults.Graft Survival: The survival of a graft in a host, the factors responsible for the survival and the changes occurring within the graft during growth in the host.Treatment Outcome: Evaluation undertaken to assess the results or consequences of management and procedures used in combating disease in order to determine the efficacy, effectiveness, safety, and practicability of these interventions in individual cases or series.Tissue Donors: Individuals supplying living tissue, organs, cells, blood or blood components for transfer or transplantation to histocompatible recipients.Cell Transplantation: Transference of cells within an individual, between individuals of the same species, or between individuals of different species.Embryonic Stem Cells: Cells derived from the BLASTOCYST INNER CELL MASS which forms before implantation in the uterine wall. They retain the ability to divide, proliferate and provide progenitor cells that can differentiate into specialized cells.Osteogenesis: The process of bone formation. Histogenesis of bone including ossification.Myeloablative Agonists: Agents that destroy bone marrow activity. They are used to prepare patients for BONE MARROW TRANSPLANTATION or STEM CELL TRANSPLANTATION.Cell Lineage: The developmental history of specific differentiated cell types as traced back to the original STEM CELLS in the embryo.Cell Proliferation: All of the processes involved in increasing CELL NUMBER including CELL DIVISION.Kidney Transplantation: The transference of a kidney from one human or animal to another.Cell Culture Techniques: Methods for maintaining or growing CELLS in vitro.Multipotent Stem Cells: Specialized stem cells that are committed to give rise to cells that have a particular function; examples are MYOBLASTS; MYELOID PROGENITOR CELLS; and skin stem cells. (Stem Cells: A Primer [Internet]. Bethesda (MD): National Institutes of Health (US); 2000 May [cited 2002 Apr 5]. Available from: http://www.nih.gov/news/stemcell/primer.htm)Chondrogenesis: The formation of cartilage. This process is directed by CHONDROCYTES which continually divide and lay down matrix during development. It is sometimes a precursor to OSTEOGENESIS.Whole-Body Irradiation: Irradiation of the whole body with ionizing or non-ionizing radiation. It is applicable to humans or animals but not to microorganisms.Busulfan: An alkylating agent having a selective immunosuppressive effect on BONE MARROW. It has been used in the palliative treatment of chronic myeloid leukemia (MYELOID LEUKEMIA, CHRONIC), but although symptomatic relief is provided, no permanent remission is brought about. According to the Fourth Annual Report on Carcinogens (NTP 85-002, 1985), busulfan is listed as a known carcinogen.Tissue Engineering: Generating tissue in vitro for clinical applications, such as replacing wounded tissues or impaired organs. The use of TISSUE SCAFFOLDING enables the generation of complex multi-layered tissues and tissue structures.Antigens, CD34: Glycoproteins found on immature hematopoietic cells and endothelial cells. They are the only molecules to date whose expression within the blood system is restricted to a small number of progenitor cells in the bone marrow.Histocompatibility Testing: Identification of the major histocompatibility antigens of transplant DONORS and potential recipients, usually by serological tests. Donor and recipient pairs should be of identical ABO blood group, and in addition should be matched as closely as possible for HISTOCOMPATIBILITY ANTIGENS in order to minimize the likelihood of allograft rejection. (King, Dictionary of Genetics, 4th ed)Multiple Myeloma: A malignancy of mature PLASMA CELLS engaging in monoclonal immunoglobulin production. It is characterized by hyperglobulinemia, excess Bence-Jones proteins (free monoclonal IMMUNOGLOBULIN LIGHT CHAINS) in the urine, skeletal destruction, bone pain, and fractures. Other features include ANEMIA; HYPERCALCEMIA; and RENAL INSUFFICIENCY.Recurrence: The return of a sign, symptom, or disease after a remission.Hematopoiesis: The development and formation of various types of BLOOD CELLS. Hematopoiesis can take place in the BONE MARROW (medullary) or outside the bone marrow (HEMATOPOIESIS, EXTRAMEDULLARY).Flow Cytometry: Technique using an instrument system for making, processing, and displaying one or more measurements on individual cells obtained from a cell suspension. Cells are usually stained with one or more fluorescent dyes specific to cell components of interest, e.g., DNA, and fluorescence of each cell is measured as it rapidly transverses the excitation beam (laser or mercury arc lamp). Fluorescence provides a quantitative measure of various biochemical and biophysical properties of the cell, as well as a basis for cell sorting. Other measurable optical parameters include light absorption and light scattering, the latter being applicable to the measurement of cell size, shape, density, granularity, and stain uptake.Time Factors: Elements of limited time intervals, contributing to particular results or situations.Combined Modality Therapy: The treatment of a disease or condition by several different means simultaneously or sequentially. Chemoimmunotherapy, RADIOIMMUNOTHERAPY, chemoradiotherapy, cryochemotherapy, and SALVAGE THERAPY are seen most frequently, but their combinations with each other and surgery are also used.Stem Cell Niche: A particular zone of tissue composed of a specialized microenvironment where stem cells are retained in a undifferentiated, self-renewable state.Hematopoietic Stem Cell Mobilization: The release of stem cells from the bone marrow into the peripheral blood circulation for the purpose of leukapheresis, prior to stem cell transplantation. Hematopoietic growth factors or chemotherapeutic agents often are used to stimulate the mobilization.Remission Induction: Therapeutic act or process that initiates a response to a complete or partial remission level.Leukemia: A progressive, malignant disease of the blood-forming organs, characterized by distorted proliferation and development of leukocytes and their precursors in the blood and bone marrow. Leukemias were originally termed acute or chronic based on life expectancy but now are classified according to cellular maturity. Acute leukemias consist of predominately immature cells; chronic leukemias are composed of more mature cells. (From The Merck Manual, 2006)Immunosuppressive Agents: Agents that suppress immune function by one of several mechanisms of action. Classical cytotoxic immunosuppressants act by inhibiting DNA synthesis. Others may act through activation of T-CELLS or by inhibiting the activation of HELPER CELLS. While immunosuppression has been brought about in the past primarily to prevent rejection of transplanted organs, new applications involving mediation of the effects of INTERLEUKINS and other CYTOKINES are emerging.Pluripotent Stem Cells: Cells that can give rise to cells of the three different GERM LAYERS.Fetal Stem Cells: Cells derived from a FETUS that retain the ability to divide, proliferate and provide progenitor cells that can differentiate into specialized cells.Histocompatibility: The degree of antigenic similarity between the tissues of different individuals, which determines the acceptance or rejection of allografts.Retrospective Studies: Studies used to test etiologic hypotheses in which inferences about an exposure to putative causal factors are derived from data relating to characteristics of persons under study or to events or experiences in their past. The essential feature is that some of the persons under study have the disease or outcome of interest and their characteristics are compared with those of unaffected persons.Melphalan: An alkylating nitrogen mustard that is used as an antineoplastic in the form of the levo isomer - MELPHALAN, the racemic mixture - MERPHALAN, and the dextro isomer - MEDPHALAN; toxic to bone marrow, but little vesicant action; potential carcinogen.Adipogenesis: The differentiation of pre-adipocytes into mature ADIPOCYTES.Cell SeparationRegeneration: The physiological renewal, repair, or replacement of tissue.Graft vs Leukemia Effect: Immunological rejection of leukemia cells following bone marrow transplantation.Tissue Scaffolds: Cell growth support structures composed of BIOCOMPATIBLE MATERIALS. They are specially designed solid support matrices for cell attachment in TISSUE ENGINEERING and GUIDED TISSUE REGENERATION uses.Cell- and Tissue-Based Therapy: Therapies that involve the TRANSPLANTATION of CELLS or TISSUES developed for the purpose of restoring the function of diseased or dysfunctional cells or tissues.Heart Transplantation: The transference of a heart from one human or animal to another.Mice, Inbred C57BLCoculture Techniques: A technique of culturing mixed cell types in vitro to allow their synergistic or antagonistic interactions, such as on CELL DIFFERENTIATION or APOPTOSIS. Coculture can be of different types of cells, tissues, or organs from normal or disease states.Neural Stem Cells: Self-renewing cells that generate the main phenotypes of the nervous system in both the embryo and adult. Neural stem cells are precursors to both NEURONS and NEUROGLIA.Chimerism: The occurrence in an individual of two or more cell populations of different chromosomal constitutions, derived from different individuals. This contrasts with MOSAICISM in which the different cell populations are derived from a single individual.Vidarabine: A nucleoside antibiotic isolated from Streptomyces antibioticus. It has some antineoplastic properties and has broad spectrum activity against DNA viruses in cell cultures and significant antiviral activity against infections caused by a variety of viruses such as the herpes viruses, the VACCINIA VIRUS and varicella zoster virus.Umbilical Cord: The flexible rope-like structure that connects a developing FETUS to the PLACENTA in mammals. The cord contains blood vessels which carry oxygen and nutrients from the mother to the fetus and waste products away from the fetus.Disease-Free Survival: Period after successful treatment in which there is no appearance of the symptoms or effects of the disease.Transplantation Immunology: A general term for the complex phenomena involved in allo- and xenograft rejection by a host and graft vs host reaction. Although the reactions involved in transplantation immunology are primarily thymus-dependent phenomena of cellular immunity, humoral factors also play a part in late rejection.Survival Rate: The proportion of survivors in a group, e.g., of patients, studied and followed over a period, or the proportion of persons in a specified group alive at the beginning of a time interval who survive to the end of the interval. It is often studied using life table methods.Survival Analysis: A class of statistical procedures for estimating the survival function (function of time, starting with a population 100% well at a given time and providing the percentage of the population still well at later times). The survival analysis is then used for making inferences about the effects of treatments, prognostic factors, exposures, and other covariates on the function.Fetal Blood: Blood of the fetus. Exchange of nutrients and waste between the fetal and maternal blood occurs via the PLACENTA. The cord blood is blood contained in the umbilical vessels (UMBILICAL CORD) at the time of delivery.Induced Pluripotent Stem Cells: Cells from adult organisms that have been reprogrammed into a pluripotential state similar to that of EMBRYONIC STEM CELLS.Leukemia, Myeloid, Acute: Clonal expansion of myeloid blasts in bone marrow, blood, and other tissue. Myeloid leukemias develop from changes in cells that normally produce NEUTROPHILS; BASOPHILS; EOSINOPHILS; and MONOCYTES.Graft Rejection: An immune response with both cellular and humoral components, directed against an allogeneic transplant, whose tissue antigens are not compatible with those of the recipient.Lung Transplantation: The transference of either one or both of the lungs from one human or animal to another.Bone Marrow: The soft tissue filling the cavities of bones. Bone marrow exists in two types, yellow and red. Yellow marrow is found in the large cavities of large bones and consists mostly of fat cells and a few primitive blood cells. Red marrow is a hematopoietic tissue and is the site of production of erythrocytes and granular leukocytes. Bone marrow is made up of a framework of connective tissue containing branching fibers with the frame being filled with marrow cells.Cyclophosphamide: Precursor of an alkylating nitrogen mustard antineoplastic and immunosuppressive agent that must be activated in the LIVER to form the active aldophosphamide. It has been used in the treatment of LYMPHOMA and LEUKEMIA. Its side effect, ALOPECIA, has been used for defleecing sheep. Cyclophosphamide may also cause sterility, birth defects, mutations, and cancer.Graft vs Tumor Effect: Immunological rejection of tumor tissue/cells following bone marrow transplantation.Antineoplastic Combined Chemotherapy Protocols: The use of two or more chemicals simultaneously or sequentially in the drug therapy of neoplasms. The drugs need not be in the same dosage form.Hepatic Veno-Occlusive Disease: Liver disease that is caused by injuries to the ENDOTHELIAL CELLS of the vessels and subendothelial EDEMA, but not by THROMBOSIS. Extracellular matrix, rich in FIBRONECTINS, is usually deposited around the HEPATIC VEINS leading to venous outflow occlusion and sinusoidal obstruction.Cell Survival: The span of viability of a cell characterized by the capacity to perform certain functions such as metabolism, growth, reproduction, some form of responsiveness, and adaptability.Regenerative Medicine: A field of medicine concerned with developing and using strategies aimed at repair or replacement of damaged, diseased, or metabolically deficient organs, tissues, and cells via TISSUE ENGINEERING; CELL TRANSPLANTATION; and ARTIFICIAL ORGANS and BIOARTIFICIAL ORGANS and tissues.Salvage Therapy: A therapeutic approach, involving chemotherapy, radiation therapy, or surgery, after initial regimens have failed to lead to improvement in a patient's condition. Salvage therapy is most often used for neoplastic diseases.Osteoblasts: Bone-forming cells which secrete an EXTRACELLULAR MATRIX. HYDROXYAPATITE crystals are then deposited into the matrix to form bone.Siblings: Persons or animals having at least one parent in common. (American College Dictionary, 3d ed)Myelodysplastic Syndromes: Clonal hematopoietic stem cell disorders characterized by dysplasia in one or more hematopoietic cell lineages. They predominantly affect patients over 60, are considered preleukemic conditions, and have high probability of transformation into ACUTE MYELOID LEUKEMIA.HLA Antigens: Antigens determined by leukocyte loci found on chromosome 6, the major histocompatibility loci in humans. They are polypeptides or glycoproteins found on most nucleated cells and platelets, determine tissue types for transplantation, and are associated with certain diseases.Cell Tracking: Non-invasive imaging of cells that have been labeled non-destructively, such as with nanoemulsions or reporter genes that can be detected by molecular imaging, to monitor their location, viability, cell lineage expansion, response to drugs, movement, or other behaviors in vivo.Hematologic Diseases: Disorders of the blood and blood forming tissues.Transplantation, Isogeneic: Transplantation between genetically identical individuals, i.e., members of the same species with identical histocompatibility antigens, such as monozygotic twins, members of the same inbred strain, or members of a hybrid population produced by crossing certain inbred strains.Organ Transplantation: Transference of an organ between individuals of the same species or between individuals of different species.Transplantation, Heterologous: Transplantation between animals of different species.Islets of Langerhans Transplantation: The transference of pancreatic islets within an individual, between individuals of the same species, or between individuals of different species.Granulocyte Colony-Stimulating Factor: A glycoprotein of MW 25 kDa containing internal disulfide bonds. It induces the survival, proliferation, and differentiation of neutrophilic granulocyte precursor cells and functionally activates mature blood neutrophils. Among the family of colony-stimulating factors, G-CSF is the most potent inducer of terminal differentiation to granulocytes and macrophages of leukemic myeloid cell lines.Immunosuppression: Deliberate prevention or diminution of the host's immune response. It may be nonspecific as in the administration of immunosuppressive agents (drugs or radiation) or by lymphocyte depletion or may be specific as in desensitization or the simultaneous administration of antigen and immunosuppressive drugs.Lymphocyte Transfusion: The transfer of lymphocytes from a donor to a recipient or reinfusion to the donor.Reverse Transcriptase Polymerase Chain Reaction: A variation of the PCR technique in which cDNA is made from RNA via reverse transcription. The resultant cDNA is then amplified using standard PCR protocols.Stem Cell Factor: A hematopoietic growth factor and the ligand of the cell surface c-kit protein (PROTO-ONCOGENE PROTEINS C-KIT). It is expressed during embryogenesis and is a growth factor for a number of cell types including the MAST CELLS and the MELANOCYTES in addition to the HEMATOPOIETIC STEM CELLS.Colony-Forming Units Assay: A cytologic technique for measuring the functional capacity of stem cells by assaying their activity.Unrelated Donors: Providers of tissues for transplant to non-related individuals.Follow-Up Studies: Studies in which individuals or populations are followed to assess the outcome of exposures, procedures, or effects of a characteristic, e.g., occurrence of disease.Immunophenotyping: Process of classifying cells of the immune system based on structural and functional differences. The process is commonly used to analyze and sort T-lymphocytes into subsets based on CD antigens by the technique of flow cytometry.Mice, SCID: Mice homozygous for the mutant autosomal recessive gene "scid" which is located on the centromeric end of chromosome 16. These mice lack mature, functional lymphocytes and are thus highly susceptible to lethal opportunistic infections if not chronically treated with antibiotics. The lack of B- and T-cell immunity resembles severe combined immunodeficiency (SCID) syndrome in human infants. SCID mice are useful as animal models since they are receptive to implantation of a human immune system producing SCID-human (SCID-hu) hematochimeric mice.Leukemia, Myelogenous, Chronic, BCR-ABL Positive: Clonal hematopoetic disorder caused by an acquired genetic defect in PLURIPOTENT STEM CELLS. It starts in MYELOID CELLS of the bone marrow, invades the blood and then other organs. The condition progresses from a stable, more indolent, chronic phase (LEUKEMIA, MYELOID, CHRONIC PHASE) lasting up to 7 years, to an advanced phase composed of an accelerated phase (LEUKEMIA, MYELOID, ACCELERATED PHASE) and BLAST CRISIS.Transplantation: Transference of a tissue or organ from either an alive or deceased donor, within an individual, between individuals of the same species, or between individuals of different species.T-Lymphocytes: Lymphocytes responsible for cell-mediated immunity. Two types have been identified - cytotoxic (T-LYMPHOCYTES, CYTOTOXIC) and helper T-lymphocytes (T-LYMPHOCYTES, HELPER-INDUCER). They are formed when lymphocytes circulate through the THYMUS GLAND and differentiate to thymocytes. When exposed to an antigen, they divide rapidly and produce large numbers of new T cells sensitized to that antigen.Lymphoma, Non-Hodgkin: Any of a group of malignant tumors of lymphoid tissue that differ from HODGKIN DISEASE, being more heterogeneous with respect to malignant cell lineage, clinical course, prognosis, and therapy. The only common feature among these tumors is the absence of giant REED-STERNBERG CELLS, a characteristic of Hodgkin's disease.Gene Expression Regulation: Any of the processes by which nuclear, cytoplasmic, or intercellular factors influence the differential control (induction or repression) of gene action at the level of transcription or translation.Antigens, CD: Differentiation antigens residing on mammalian leukocytes. CD stands for cluster of differentiation, which refers to groups of monoclonal antibodies that show similar reactivity with certain subpopulations of antigens of a particular lineage or differentiation stage. The subpopulations of antigens are also known by the same CD designation.Biological Markers: Measurable and quantifiable biological parameters (e.g., specific enzyme concentration, specific hormone concentration, specific gene phenotype distribution in a population, presence of biological substances) which serve as indices for health- and physiology-related assessments, such as disease risk, psychiatric disorders, environmental exposure and its effects, disease diagnosis, metabolic processes, substance abuse, pregnancy, cell line development, epidemiologic studies, etc.Precursor Cell Lymphoblastic Leukemia-Lymphoma: A neoplasm characterized by abnormalities of the lymphoid cell precursors leading to excessive lymphoblasts in the marrow and other organs. It is the most common cancer in children and accounts for the vast majority of all childhood leukemias.Antilymphocyte Serum: Serum containing GAMMA-GLOBULINS which are antibodies for lymphocyte ANTIGENS. It is used both as a test for HISTOCOMPATIBILITY and therapeutically in TRANSPLANTATION.Cell Shape: The quality of surface form or outline of CELLS.Cell Movement: The movement of cells from one location to another. Distinguish from CYTOKINESIS which is the process of dividing the CYTOPLASM of a cell.Hematopoietic System: The blood-making organs and tissues, principally the bone marrow and lymph nodes.

*Stem cell transplantation for articular cartilage repair

Mesenchymal stem cells (MSCs) are multipotent cells found in multiple human adult tissues including bone marrow, synovial ... 2003). "The biology of hematopoietic stem cells". Arch Med Res. 34 (6): 446-60. doi:10.1016/j.arcmed.2003.06.004. PMID 14734085 ... and nucleated cells. The adult stem cell fraction is present in the nucleated cells of the marrow. Most of these cells are ... As a result, many researchers have focused on adult stem cells [1], or stem cells isolated from adult humans that can be ...

*Adult stem cell

... has published a small case series of nine defects in five knees involving surgical transplantation of mesenchymal stem cells ... Such cells are one of the various classes of induced stem cells. Hematopoietic stem cells are found in the bone marrow and ... such as mesenchymal stem cell, adipose-derived stem cell, endothelial stem cell, etc.). A great deal of adult stem cell ... Intestinal stem cells reside near the base of the stem cell niche, called the crypts of Lieberkuhn. Intestinal stem cells are ...

*Muse cell

Mesenchymal stem cell transplantation: Muse cells exist within cultured MSCs such as bone marrow mesenchymal stem cells and ... Muse cells do not express CD34 (markers for hematopoietic stem cells, adipose stem cells, VSELs) and CD117 (hematopoietic stem ... When mesenchymal cells (sometimes called mesenchymal stem cells) are separated into Muse and non-Muse cells by SSEA-3 cell ... bone marrow-mesenchymal stem cells and adipose-derived stem cells. Muse cells are able to generate cells representative of all ...

*Guo Mei

... stem cell transplantation 2007 Effects of mesenchymal stem cells on cell cycle and apoptosis of hematopoietic tissue cells in ... blood stem cell transplantation 2005 Establishment of a rhesus haploidentical hematopoietic stem cell and mesenchymal stem cell ... stem cell transplantation and mesenchymal stem cells 2013 Haploidentical nonmyeloablative allogeneic peripheral blood stem cell ... stem cell transplantation 2003 The clinical research of nonmyeloablative allogeneic peripheral blood hematopoietic stem cells ...

*Stem-cell therapy

... also published a small case series of nine defects in five knees involving surgical transplantation of mesenchymal stem cells ... Stem cells are also in clinical phases for treatment in ophthalmology. Hematopoietic stem cells have been used to treat corneal ... Fetal tissue implant Human Stem Cells Institute Induced pluripotent stem cell Induced stem cells Ian Murnaghan for Explore Stem ... however other types of adult stem cells may be used, such as adipose-derived stem cells. Adult stem cell therapy for treating ...

*Cord blood

... hematopoietic cell transplantation stem cell transplantation (4th ed.). Oxford: Wiley-Blackwell. ISBN 9781444303537. Haller M J ... Perspective on the Application of Mesenchymal Stem Cells and Their Secretion Products". Stem Cells International. 2016: 9756973 ... It contains red blood cells, white blood cells, plasma, platelets and is also rich in hematopoietic stem cells. There are ... Cord blood is used the same way that hematopoietic stem cell transplantation is used to reconstitute bone marrow following ...

*Cell therapy

2005). "Fetal mesenchymal stem-cell engraftment in bone after in utero transplantation in a patient with severe osteogenesis ... 2001). "Sonic hedgehog induces the proliferation of primitive human hematopoietic cells via BMP regulation". Nature Immunology ... "Mesenchymal stem cells: Mechanisms of immunomodulation and homing". Cell Transplant. 19 (6): 667-79. doi:10.3727/ ... "The Impact of Market Volatility on the Cell Therapy Industry". Cell Stem Cell. 9 (5): 397-401. doi:10.1016/j.stem.2011.10.010. ...

*Graft-versus-host disease

... when compared to oral cancer in non-hematopoietic stem cell transplantation patients. In the clinical setting, graft-versus- ... The trial was of a mesenchymal stem cell therapy known as remestemcel-L or MSC-100-IV. Survival rate was 82% (vs 39% of ... The use of liver-derived hematopoietic stem cells to reconstitute bone marrow has the highest success rate according to recent ... "Oral Cancer in Patients After Hematopoietic Stem-Cell Transplantation: Long-Term Follow-Up Suggests an Increased Risk for ...

*Stem cell

... mesenchymal stem cell, adipose-derived stem cell, endothelial stem cell, dental pulp stem cell, etc.). Muse cells (multi- ... 16 December 2013 Why Perform a Stem Cell Transplant? Bone Marrow Transplantation and Peripheral Blood Stem Cell Transplantation ... For example, the defining test for bone marrow or hematopoietic stem cells (HSCs) is the ability to transplant the cells and ... Cell bank Human genome Meristem Partial cloning Plant stem cell Stem cell controversy Stem cell marker Shinya Yamanaka Tuch BE ...

*Stem cell marker

... cells in parental-to-F1 hematopoietic chimeras that restored normal stem cell concentration after initial transplantation". ... "Undifferentiated mouse mesenchymal stem cells spontaneously express neural and stem cell markers Oct-4 and Rex-1". Cytotherapy ... Stem cell markers are genes and their protein products used by scientists to isolate and identify stem cells. Stem cells can ... 2005). "Somatic stem cell marker prominin-1/CD133 is expressed in embryonic stem cell-derived progenitors". Stem Cells. 23 (6 ...

*Cardiomyoplasty

These cell populations are endothelial progenitor cells, hematopoietic stem cells and mesenchymal stem cells. Adipose tissue ... Matsuura K, Haraguchi Y, Shimizu T, Okano T (2013). "Cell sheet transplantation for heart tissue repair". J Control Release. ... Subcutaneous adipose tissue is also a source of mesenchymal stem cells and have demonstrated positive outcomes in terms of ... Hence, research has been focused on induced pluripotent stem cells (iPSCs) from somatic human tissue. Further to cell and ...

*Stem-cell niche

SDF-1α secreted from Mesenchymal Stem Cells (MSCs) has important role in homing and maintenance of Hematopoetic Stem Cell (HSC ... Vertebrate hematopoietic stem cells niche in the bone marrow is formed by cells subendosteal osteoblasts, sinusoidal ... "IL-22 Administration Protects Intestinal Stem Cells from Gvhd". Biology of Blood and Marrow Transplantation. 20: S53-S54. doi: ... cell-cell interactions between stem cells, as well as interactions between stem cells and neighbouring differentiated cells, ...

*Induced stem cells

"Induced Pluripotent Stem Cells from Human Hair Follicle Mesenchymal Stem Cells". Stem Cell Reviews and Reports. 9 (4): 451-60. ... "Generation of Engraftable Hematopoietic Stem Cells from Induced Pluripotent Stem Cells by Way of Teratoma Formation". Molecular ... This reduces their value as a source for autologous stem cell transplantation therapy, as for the present, it is not clear ... link) Scientists develop 'game changing' stem cell repair system. Stem Cells Portal Could this new stem cell become the game ...

*Pluristem Therapeutics

... its Phase I trial of PLX-R18 cells to treat incomplete hematopoietic recovery following Hematopoietic Cell Transplantation. The ... Nevada corporation Cell therapy Mesenchymal stem cell Acute limb ischemia Buerger's disease List of Israeli companies quoted on ... "Pluristem stem cell therapy saves a patient, shares jump". The Baltimore Sun. Reuters. Retrieved 9 May 2012. "Pluristem stem ... איך הצילו תאי הגזע של פלוריסטם את חייה של ילדה בת 7? [How did Pluristem's stem cells save the life of a 7-year-old girl?]. ...

*CD34

... + cell) are normally found in the umbilical cord and bone marrow as hematopoietic cells, or in mesenchymal stem cells, ... are used to quantify and purify hematopoietic progenitor stem cells for research and for clinical bone marrow transplantation. ... in a cell surface glycoprotein and functions as a cell-cell adhesion factor. It may also mediate the attachment of stem cells ... It is important to mention that Long-Term Hematopoietic Stem Cells (LT-HSCs) in mice and humans are the hematopoietic cells ...

*Organoid

... stem cell-derived tissue-specific progenitors or relevant tissue samples with endothelial cells and mesenchymal stem cells. ... as co-culturing human hematopoietic or bone marrow stem cells with mouse thymic organoids resulted in the production of T-cells ... "IL-22 Administration Protects Intestinal Stem Cells from Gvhd". Biology of Blood and Marrow Transplantation. 20 (2): S53-S54. ... They are derived from one or a few cells from a tissue, embryonic stem cells or induced pluripotent stem cells, which can self- ...

*Mesenchymal stem cell

These bone marrow stem cells do not contribute to the formation of blood cells so do not express the hematopoietic stem cell ... Many of the early clinical successes using intravenous transplantation came in systemic diseases such as graft versus host ... Mesenchymal stem cells fact sheet, published June 2012, scientist-reviewed and not too technical Mesenchymal Stem Cell Research ... Mesenchymal stem cells are characterized morphologically by a small cell body with a few cell processes that are long and thin ...

*Marrow adipose tissue

"Fate decision of mesenchymal stem cells: adipocytes or osteoblasts?". Cell Death & Differentiation. 23 (7): 1128-1139. doi: ... These hematopoietic cells are derived from hematopoietic stem cells (HSC) which give rise to diverse cells: cells of the blood ... As such cancers are often treated with bone marrow transplantation, there is interest in improving the renewal of HSC. Recent ... Hematopoietic cells (also known as blood cells) reside in the bone marrow along with marrow adipocytes. ...

*Alexander A. Maximow

Cell Ther Transplant. 2009;1:e.000033.01. doi:10.3205/ctt-2009-en-000033.01 Friedenstein A. On stromal-hematopoietic ... Translation into English: The Lymphocyte as a stem cell common to different blood elements in embryonic development and during ... After demonstrating that all blood cells develop from a common precursor cell, Maximow confirmed the unitarian theory of ... Biography of William Bloom Written by Ronald Singer Cellular Therapy and Transplantation, Volume 1, Number 3, December 2009 - ...

*Cell isolation

Isolation of Haematopoetic stem cells: Rector K, Liu Y, Van Zant G. "Comprehensive hematopoietic stem cell isolation methods". ... Isolation of Mesenchymal stem cells: Yosupov N, Haimov H, Juodzbalys G. "Mobilization, Isolation and Characterization of Stem ... Isolation of pancreatic islet cells, followed by their subsequent culture and transplantation, has been used to treat patients ... Isolated cells can also be used for cell culture, in which a single cell multiplies to create a colony of cells.[citation ...

*Regenerative medicine

... hematopoietic cell transplantation stem cell transplantation (4th ed.). Oxford: Wiley-Blackwell. ISBN 9781444303537. Haller M J ... Perspective on the Application of Mesenchymal Stem Cells and Their Secretion Products". Stem cells international. 2016: 9756973 ... which can differentiate only into blood cells), and not pluripotent stem cells (such as embryonic stem cells, which can ... Any such potential beyond blood and immunological uses is limited by the fact that cord cells are hematopoietic stem cells ( ...

*Microchimerism

... although several different cell types have been identified, such as various immune lineages, mesenchymal stem cells, and ... "Fetomaternal Microchimerism Is Associated with Better Outcome in Haploidentical Hematopoietic Stem Cell Transplantation". Blood ... Isolating cells in cultures can alter the properties of the stem cells, but in pregnancy the effects of fetal stem cells can be ... show similarities to graft vs host disease which occurs after hematopoietic stem cell transplants. Fetal cells colonize ...

*Desmoplastic small-round-cell tumor

Other treatment options include: hematopoietic stem cell transplantation, intensity-modulated radiation Therapy, radiofrequency ... On immunohistochemistry, these cells have trilinear coexpression including the epithelial marker cytokeratin, the mesenchymal ... In dogs, mast cell tumors are the most frequent round cell tumor. There are few early warning signs that a patient has a DSRCT ... A multi-modality approach of high-dose chemotherapy, aggressive surgical resection, radiation, and stem cell rescue improves ...

*Embryoid body

... and BMP Signaling Regulate Distinct Stages in the Developmental Pathway from Embryonic Stem Cells to Blood". Cell Stem Cell. 2 ... Wiles, M. V.; Keller, G. (1991). "Multiple hematopoietic lineages develop from embryonic stem (ES) cells in culture". ... and mesenchymal-like cell populations, as well as the appearance of markers associated with the epithelial-mesenchymal ... Briggs, R.; King, T. J. (1952). "Transplantation of Living Nuclei from Blastula Cells into Enucleated Frogs' Eggs". Proceedings ...

*Epigenetic clock

Hematopoietic stem cell transplantation, which transplants these cells from a young donor to an older recipient, rejuvenates ... glial cells, neurons, immortalized B cells, mesenchymal stromal cells. However, accuracy depends to some extent on the source ... it is close to zero for embryonic and induced pluripotent stem cells; second, it correlates with cell passage number; third, it ... It is an intrinsic mechanism that exists from the birth of the cell and continues. This implies that if cells are not shunted ...

*ETV6

These translocation mutations commonly occur in pluripotent hematopoietic stem cells that differentiate into various types of ... in a B-cell ALL cell line". Genes, Chromosomes & Cancer. 18 (4): 254-68. PMID 9087565. Van Vlierberghe P, Ambesi-Impiombato A, ... day 10.5 and 11.5 of embryonic life with defective yolk sac angiogenesis and extensive losses in mesenchymal and neural cells ... lymphoblastic leukemia with 2 among 3 family members moving rather quickly from chemotherapy to bone marrow transplantation and ...
TY - JOUR. T1 - Hypoxia-preconditioned adipose tissue-derived mesenchymal stem cell increase the survival and gene expression of engineered neural stem cells in a spinal cord injury model. AU - Oh, Jin Soo. AU - Ha, Yoon. AU - An, Sung Su. AU - Khan, Momin. AU - Pennant, William A.. AU - Kim, Hyo Jin. AU - Yoon, Do Heum. AU - Lee, Minhyung. AU - Kim, Keung Nyun. PY - 2010/3/26. Y1 - 2010/3/26. N2 - Hypoxic preconditioning (HP) is a novel strategy to make stem cells resistant to the ischemic environment they encounter after transplantation into injured tissue; this strategy improves survival of both the transplanted cells and the host cells at the injury site. Using both in vitro and in vivo injury models, we confirmed that HP-treated adipose tissue-derived ...
ATCC ® Normal Human Adipose-Derived Mesenchymal Stem Cells, when grown in Mesenchymal Stem Cell Basal Media supplemented with Mesenchymal Stem Cell Growth Kit Low serum components, provide an ideal cell system to propagate mesenchymal stem cells in low serum (2% FBS) conditions. When maintained under optimal growth conditions, ATCC Normal Human Adipose-Derived Mesenchymal Stem Cells have been shown to be multipotent, capable of differentiating down the adipogenic, osteogenic, and chondrogenic lineages. The cells are cryopreserved at the second passage to ensure the highest viability and plating efficiency. ATCC ® Primary ...
Background: Developing efficient methods to isolate and identify human adipose-derived mesenchymal stem cells (hADSCs) remains to be one of the major challenges in tissue engineering.. Methods: We demonstrate here a method by isolating hADSCs from abdominal subcutaneous adipose tissue harvested during caesarian section. The hADSCs were isolated from human adipose tissue by collagenase digestion and adherence to flasks.. Results: The yield reached around 1 x 10(6) hADSCs per gram adipose tissue. The following comprehensive identification and characterization illustrated pronounced features of mesenchymal stem cells (MSCs). The fibroblast-like hADSCs exhibited typical ultrastructure details for vigorous cell activities. Karyotype mapping showed normal human chromosome. With unique immunophenotypes they were positive for CD29, CD44, CD73, CD105 and CD166, but ...
Background: Increasing evidences indicated that adipose-derived mesenchymal stem cells (ADMSCs) can stay survive, then gradually proliferate and differentiate into myocardial cells after transplanted into damaged areas and improve function of heart.M
Background: Cell therapy is a potential therapeutic approach for neurodegenerative disorders. Human umbilical cord bloodderived mesenchymal stem cells (hUCB-MSCs) are an appropriate source of stem cells for use in various cell-based therapies. Objectives: In this study, we examined a real-time PCR approach for neural differentiation of hUCB-MSCs in vitro. Materials and Methods: MSCs were cultured in DMEM medium supplemented with 10% FBS in a humidified incubator equilibration at 5% CO2 and 37°C. For the neural differentiation of MSCs, the DMEM was removed and replaced with pre-induction media (retinoic acid [RA], basic fibroblast growth factor [bFGF], and epidermal growth factor [EGF]) and basal medium for two days. They were then cultured in nerve growth factor (NGF), 3-isobutyl-1-methylxanthine (IBMX), ascorbic acid (AA), and basal medium for six days. We ...
This study is aiming to investigate the efficacy of autologous mesenchymal stem cell (MSC) transplantation in 25 randomized patients with liver cirrhosis autologous mesenchymal stem cell will be derived from patients bone marrows and will be infused via peripheral vein. Liver biopsies will be performed in every patient in the beginning and at 6th month ...
This study will explore safety and efficacy of allogeneic mesenchymal stem cells transplantation (MSCT) to treat patients with diagnosis of primary Sjögrens Syndrome (pSS) who have been resistant to multiple standard treatments. The underlying hypothesis is that the pSS condition is caused by an abnormal immune homeostasis that can be restored by MSCT ...
In this study, after 21 days of ceasing CCl 4 , both placebo-treated and untreated cirrhotic mice showed reduced liver damage as indicated by a decrease of AST/ALT index, recovery of histology, and reduction of fibrosis-related gene expression. Presumably, the liver of these mice were capable of self-regeneration. The cause of the self-regeneration has been explained by the ceasing of CCl 4 administration during the 21 days of treatment. Other studies have shown similar results to our study Carvalho et al., 2008 Kuo et al., 2008 .. However, our study uniquely demonstrates that stem cell treatment in cirrhotic mice was better for restoring liver function and histology (significantly better than placebo treatment). The effects of stem cell treatment in our mouse model of liver cirrhosis may be attributed to stem cell immunomodulation, stem cell homing and differentiation, and ...
Bone marrow mesenchymal stem cells (MSCs) are one of the potential tools for treatment of the spinal cord injury; however, the survival and differentiation of MSCs in an injured spinal cord still need to be improved. In the present study, we investigated whether Governor Vessel electro-acupuncture (EA) could efficiently promote bone marrow mesenchymal stem cells (MSCs) survival and differentiation, axonal regeneration and finally, functional recovery in the transected spinal cord. The spinal cords of adult Sprague-Dawley (SD) rats were completely transected at T10, five experimental groups were performed: 1. sham operated control (Sham-control); 2. operated control (Op-control); 3. electro-acupuncture treatment (EA); 4. MSCs transplantation (MSCs); and 5. MSCs transplantation combined with electro-acupuncture (MSCs+EA). After 2-8 weeks of ...
ATCC ® Normal Human Umbilical Cord-Derived Mesenchymal Stem Cells, when grown in Mesenchymal Stem Cell Basal Media supplemented with Mesenchymal Stem Cell Growth Kit for Adipose and Umbilical-derived MSCs - Low serum components, provide an ideal cell system to propagate mesenchymal stem cells in low serum (2% FBS) conditions. When maintained under optimal growth conditions, ATCC Normal Human Umbilical Cord-Derived Mesenchymal Stem Cells have been shown to be multipotent, capable of differentiating down the adipogenic, osteogenic, and chondrogenic lineages. The cells are cryopreserved at the second passage to ensure the highest viability ...
Proteasome Activation Enhances Stemness and Lifespan of Human Mesenchymal Stem Cells By employing both Whartons-Jelly- and Adipose-derived human adult mesenchymal stem cells, investigators revealed a significant age-related decline in proteasome content and peptidase activities, accompanied by alterations of proteasomal complexes. [Free Radic Biol Med] Abstract Human Umbilical Cord Blood-Stem Cells Direct Macrophage Polarization and Block Inflammasome Activation to Alleviate Rheumatoid Arthritis Researchers investigated the therapeutic potential of human umbilical cord blood-derived mesenchymal stem cells (hUCB-MSCs) as a new therapeutic strategy for patients with rheumatoid arthritis and to explore the mechanisms underlying ...
TY - JOUR. T1 - Survivin Is Required for Mouse and Human Bone Marrow Mesenchymal Stromal Cell Function. AU - Singh, Pratibha. AU - Fukuda, Seiji. AU - Liu, Liqiong. AU - Chitteti, Brahmananda Reddy. AU - Pelus, Louis. PY - 2018/1/1. Y1 - 2018/1/1. N2 - Although mesenchymal stromal cells (MSCs) have significant potential in cell-based therapies, little is known about the factors that regulate their functions. While exploring regulatory molecules potentially involved in MSC activities, we found that the endogenous multifunctional factor Survivin is essential for MSC survival, expansion, lineage commitment, and migration. Pharmacological or genetic blockade of Survivin expression in mouse and human bone marrow MSC enhances caspase 3 and 7 expression and reduces proliferation resulting in fewer MSC and clonogenic colony-forming unit-fibroblasts (CFU-F), whereas ectopic Survivin overexpression in MSC results in ...
Cytotoxic T-lymphocyte-associated protein 4- (CTLA4-) modified human bone marrow-derived mesenchymal stem cells (hBMMSCs) might be promising seed cells for bone tissue engineering. However, the underlying mechanism is not clear. In the present study, we investigated whether CTLA4-modified hBMMSCs are involved in the migration of allogeneic hBMMSCs (allo-hBMMSCs) by maintaining POSTN secretion. hBMMSCs were isolated from different groups, named hBMMSCs and allo-hBMMSCs. hBMMSCs that were infected with the negative control (NC), empty adenovirus- or recombinant adenovirus-expressing CTLA4, POSTN, or CTLA4 plus the shRNA of POSTN were named NC hBMMSCs, CTLA4-modified hBMMSCs, POSTN-modified hBMMSCs, or CTLA4+shPOSTN-modified hBMMSCs, respectively. They were then cocultured with PBMCs in a 1 : 5 ratio with 2.5 |i|μ|/i|g/mL phytohemagglutinin (PHA). The coculture supernatant was ...
OBJECTIVE To explore the response of rat bone marrow mesenchymal stem cells (MSCs and calvarial osteoblasts to mechanical strain and the consequent changes of cytoskeleton F-actin. METHODS Bone marrow MSCs and calvarial osteoblasts were isolated from SD rats and cultured in vitro. Mechanical stretch was performed on passage 3 cells at 2 000 microepsilon for 0, 2, 6 and 12 hours using four-point bending system. The response of cells and the distribution of F-actin were observed using fluorescent staining under laser scanning confocal microscope and the morphological parameters were quantified using image analysis software Laserpix. RESULTS Under mechanical stretch, the fluorescent staining decreased obviously at both MSCs and osteoblasts, and F-actin filaments were rearranged and became tenuous, thinner, and abnormally distributed. The outline of nucleus ...
Ge, Y.; Zhang, Y.; Tang, Q.; Gao, J.; Yang, H.; Gao, Z.; Zhao, R.Chunhua., 2019: Mechanisms of the Immunomodulation Effects of Bone Marrow-Derived Mesenchymal Stem Cells on Facial Nerve Injury in Sprague-Dawley Rats
We refer to two articles by Duijvestein et al and Ciccocioppo et al1 2 in which the authors suggest that administration of autologous bone marrow-derived mesenchymal stem cells (MSCs) is safe and feasible in the treatment of refractory Crohns disease (CD). However, to date there are few data about allogeneic MSC transplantation (MSCT) for patients with inflammatory bowel disease (IBD).. We wish to report our experience of allogeneic MSCT in seven patients with IBD.. Infused allogeneic MSCs were obtained from the bone marrow or umbilical cord. Bone marrow was aspirated from healthy relatives of three patients. Umbilical cords were obtained from local maternity hospitals after normal deliveries. The isolated MSCs were given by intravenous infusions … ...
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Fang S, et al. Umbilical Cord-Derived Mesenchymal Stem Cell-Derived Exosomal MicroRNAs Suppress Myofibroblast Differentiation by Inhibiting the Transforming Growth Factor-b/SMAD2 Pathway During Wound Healing.(IF=5.902). ...
Purpose: : To observe the differentiation potential of SD rat bone marrow mesenchymal stem cells (MSCs) which were subretinally transplanted into laser-injured retina in vivo without differentiation inducements in vitro. Methods: : SD rat MSCs were cultivated in vitro to passage 3 and were injected into the sub-retina space of laser-injured rat retina with Bromo-Deoxyuridine (BrdU) labeling to trace the origin of the repopulating cells. After 4 weeks, eyeballs were enucleated for continuous paraffin slides. The expressions of Nestin, NeuN, GFAP, Vimentin, Rhodopsin were detected by immunofluorescence. Results: : BrdU-positive MSCs were present in mutiple locations, including photoreceptor layer, the bipolar layers and the ganglion cell layer. These cells expressed Nestin, NeuN, GFAP, Vimentin, and Rhodopsin. Conclusions: : For SD rats, transplanted MSCs in ...
Rheumatoid arthritis is a chronic systemic disease, which is characterized by chronic inflammation in the synovial tissue. Rheumatoid arthritis will eventually result in the destruction of cartilage, bone and ligaments and joint deformity. The underlying hypothesis is that umbilical cord-derived mesenchymal stem cell (UC-MSCs) has anti-inflammatory effects and thus potentially alleviates the progression of rheumatoid arthritis. The study is to explore the safety and efficacy of UC-MSCs transplantation in treatment of rheumatoid arthritis ...
Intranasal Administration of Mesenchymoangioblast-Derived Mesenchymal Stem Cells Abrogates Airway Fibrosis and Airway Hyperresponsiveness Associated with Chronic Allergic Airways Disease Researchers assessed the in vivo efficacy of induced pluripotent stem cell and mesenchymoangioblast-derived mesenchymal stem cells (MCA-MSCs) on airway remodeling in a murine model of chronic allergic airways disease/asthma. Apart from epithelial thickness, all other parameters measured were significantly, although not totally, decreased by intravenous delivery of MCA-MSCs to ovalbumin-injured mice. [FASEB J] Abstract Identification and Characterization of a Rich Population of CD34+ Mesenchymal Stem/Stromal Cells in Human Parotid, Sublingual and Submandibular Glands Very little is known ...
Toxic shock syndrome (TSS) is caused by an overwhelming host-mediated response to bacterial superantigens produced mainly by Staphylococcus aureus and Streptococcus pyogenes. TSS is characterized by aberrant activation of T cells and excessive release of pro-inflammatory cytokines ultimately resulting in capillary leak, septic shock, multiple organ dysfunction and high mortality rates. No therapeutic or vaccine has been approved by the U.S. Food and Drug Administration for TSS, and novel therapeutic strategies to improve clinical outcome are needed. Mesenchymal stromal (stem) cells (MSCs) are stromal cells capable of self-renewal and differentiation. Moreover, MSCs have immunomodulatory properties, including profound effects on activities of T cells and macrophages in specific contexts. Based on the critical role of host-derived immune ...
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Additional evidence comes from a group of researchers and clinicians from the United Kingdom who treated MS patients in the study, "Autologous Mesenchymal Stem Cells for the Treatment of Secondary Progressive Multiple Sclerosis: An Open-Label Phase 2a Proof-of-Concept Study.". Research findings show that intravenous administration of autologous mesenchymal stem cells to patients with secondary progressive multiple sclerosis is feasible and safe and suggests structural, functional, and physiological improvement in patients after receiving treatment with autologous mesenchymal stem cells which is consistent with remyelination. In addition, patients experienced enhanced visual acuity.. Dr. Andre Lallande, Medical Director of StemGenex Medical Group and Principal Investigator of the ...
Abstract Umbilical cord mesenchymal stem cells (MSCs) are a unique, accessible, and non-controversial source of early stem cells that can be readily manipulated. As the most common pluripotent cell, bone marrow-derived MSCs display limitations with the progress of stem cell therapy. By contrast, umbilical cord-derived cells, which have plentiful resources, are more accessible. However, several uncertain aspects, such as the effect of donor selection or culture conditions, long-term therapeutic effects, product consistency, and potential tumorigenicity, are the bottleneck in this clinical therapy. MSCs are predicted to undergo an unprecedented development in clinical treatment when a generally acknowledged criterion emerges. In the current paper, we highlight the application of umbilical cord-derived MSCs in ...
Yu, B., Zhao, X., Yang, C., Crane, J., Xian, L., Lu, W., Wan, M. and Cao, X. (2012), Parathyroid hormone induces differentiation of mesenchymal stromal/stem cells by enhancing bone morphogenetic protein signaling. J Bone Miner Res, 27: 2001-2014. doi: 10.1002/jbmr.1663 ...
Mesenchymal stem cells that come from different cell sources can look similar but behave differently. Bone marrow-derived mesenchymal stem cells tend to be the gold standard for isolating and using mesenchymal stem cells, it is not particularly easy to access these cells from the bone marrow. Because there are other, much more easily accessible mesenchymal stem cells, such as those from the umbilical cord, it is important to establish the differences between the different types of stem cells so that each can be used when most appropriate and when most advantageous.. One important difference is how to isolate the ...
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Apparently, distinct from other types of stem cells, with allogeneic mesenchymal stem cells there is no danger of the childs body rejecting the stem cells given to them. This is owed to the fact that these types of cells have no antigenicity. This remarkable attribute eradicates the need to use certain drugs that suppress the immune system and can be needed with some stem cell treatments. Cord blood, or CD34+, is known to stimulate living/normal cells and tissue causing them to work at a higher level of functioning.. In Conclusion. No matter how well known or unknown, the fact is that this treatment is used worldwide. Stem Cell Treatment is being very thoroughly studied from the U.S. to ...
TY - JOUR. T1 - Intrathecal administration of autologous mesenchymal stem cells in multiple system atrophy. AU - Singer, Wolfgang. AU - Dietz, Allan B.. AU - Zeller, Anita D.. AU - Gehrking, Tonette L.. AU - Schmelzer, James D.. AU - Schmeichel, Ann M.. AU - Gehrking, Jade A.. AU - Suarez, Mariana D.. AU - Sletten, David M.. AU - Minota Pacheco, Karla V.. AU - Coon, Elizabeth A.. AU - Sandroni, Paola. AU - Benarroch, Eduardo E.. AU - Fealey, Robert D.. AU - Matsumoto, Joseph Y.. AU - Bower, James H.. AU - Hassan, Anhar. AU - McKeon, Andrew. AU - Windebank, Anthony J.. AU - Mandrekar, Jay N.. AU - Low, Phillip A.. PY - 2019/7/2. Y1 - 2019/7/2. N2 - OBJECTIVE: This phase I/II study sought to explore intrathecal administration of mesenchymal stem cells (MSCs) as therapeutic approach to multiple system atrophy (MSA). METHODS: Utilizing a ...
Human umbilical cord mesenchymal stem cells exhibit the potential to differentiate into neural cells, which have been confirmed in in vivo and in vitro experiments. There have been few clinical reports describing umbilical cord mesenchymal stem cells for treatment of cerebral hemorrhage.. To investigate the effects of hUC-MSC treatment for cerebral hemorrhage sequela, 20 patients with cerebral hemorrhage will be enrolled and receive 4 times of hUC-MSC transplantation.. ...
Successful bone tissue engineering strategy relies on robust cell culturing technique as well as profitable cellular microenvironment. For the current clinical treatment, the expansion of cells in vitro has been a promising alternative method for the bone tissue engineering strategy. In order to enhance the culture efficiency, bioreactor systems are widely researched for the different clinical purposes. Currently, rotating wall bioreactors, biaxial bioreactors, perfusion bioreactors and spinner flask systems should be widely used in the experiments and each of them should has its own advantages and disadvanges. In this project, I would like to achieve the enhanced efficiency of human fetal mesenchymal stem cells (hfMSCs) by the parametric studies in biaxial bioreactor systems for the bone tissue engineering. The hypothesis ...
Cell therapy is a potential therapeutic approach for neurodegenerative disorders, such as Alzheimer disease (AD). Neuronal differentiation of stem cells before transplantation is a promising procedure for cell therapy. However, the therapeutic impact and mechanisms of action of neuron-like cells differentiated from human umbilical cord mesenchymal stem cells in AD have not been determined. In this study, we used tricyclodecan-9-yl-xanthogenate (D609) to induce human mesenchymal stem cells isolated from Wharton jelly of the umbilical cord (HUMSCs) to differentiate into neuron-like cells (HUMSC-NCs), and transplanted the HUMSC-NCs into an AβPP/PS1 transgenic AD mouse model. The effects of HUMSC-NC ...
Exosomes secreted by bone marrow mesenchymal stem cells (BMSCs) promote osteosarcoma cell proliferation and migration, while the underlying mechanism remains unknown. Since the long non-coding RNA PVT1 has been reported to be upregulated in osteosarcoma cells and contributes to its growth and metastasis, we aim to investigate whether BMSC-derived exosomes promote osteosarcoma growth and metastasis via transporting PVT1 into osteosarcoma cells. The PVT1 expression in BMSC-derived exosomes was markedly higher than that in osteosarcoma cell-derived exosomes. The co-culturing of BMSC-derived exosomes and osteosarcoma cells (Saos-2, MG-63, and MNNG/HOS cell lines) significantly raised PVT1 expression of osteosarcoma cells. The direct binding between PVT1 and the ...
Stem Cells International is a peer-reviewed, Open Access journal that publishes original research articles, review articles, and clinical studies in all areas of stem cell biology and applications. The journal will consider basic, translational, and clinical research, including animal models and clinical trials.
The immunomodulatory properties of type 2 diabetic patients adipose-derived mesenchymal stem cells (D-ASCs) has not been extensively studied. In this study, we compared the immunomodulatory properties of D-ASCs and non-diabetic subjects mesenchymal stem cells (ND-ASCs) in co-culture with mixed leukocyte reaction (MLR). ASCs were isolated from adipose tissue samples of type 2 diabetic and non-diabetic subjects (age: 40-55). D-ASCs and ND-ASCs were co-cultured with two-way MLR. Peri pheral blood mononuclear ce lls (PBMCs) proliferation ratio, protein levels of IFN- γ and IL-10, mRNA expression of COX-2 , TNF- α , TGF- β 1 and IL-6 genes in MLR, D-ASCs and ND-ASCs co-cultures were assessed using XTT, ELISA and Real-time qRT-PCR, respectively. PBMCs proliferation on days 2 and 4 of D-ASCs co-culture was higher than ND-ASCs co-culture of the same days ( p , 0.001). IFN- γ level ...
Background: First-generation autologous chondrocyte implantation has limitations, and introducing new effective cell sources can improve cartilage repair. Purpose: This study was conducted to compare the clinical outcomes of patients treated with first-generation autologous chondrocyte implantation to patients treated with autologous bone marrow-derived mesenchymal stem cells (BMSCs). Study Design: Cohort study; Level of evidence, 3. Methods: Seventy-two matched (lesion site and age) patients underwent cartilage repair using chondrocytes (n = 36) or BMSCs (n = 36). Clinical outcomes were measured before operation and 3, 6, 9, 12, 18, and 24 months after operation using the International Cartilage Repair Society (ICRS) Cartilage Injury Evaluation Package, which included questions from the Short-Form Health Survey, International Knee Documentation Committee (IKDC) subjective knee evaluation form, Lysholm knee scale, and Tegner ...
Discover Miltenyi Biotecs solutions for the isolation, expansion, and differentiation of mesenchymal stem cells (MSCS), including immunomodulation assays. - San Marino
Although recent studies have demonstrated the in vitro hepatic differentiation potential of mesenchymal stem cells (MSCs), the evidence supporting the in vivo engraftment of MSCs, hepatic differentiation and improvement of hepatic function is still lacking. We investigated in vivo hepatic differentiation potential and therapeutic effect of cord blood derived-MSCs (CBMSCs) transplantation in a cirrhotic rat model. CBMSCs (2x106) were infused in Wistar rats with thioacetamide-induced chronic liver injury. Biochemical markers, liver fibrosis and engraftment of CBMSCs were assessed. Infused CBMSCs were detected in the perivascular or fibrous region of the liver and did not acquire mature hepatic phenotypes. There was no difference in biochemical markers and in the area of liver fibrosis between the experimental and placebo groups. After infusion of CBMSCs in our experimental cirrhotic rat model we did not observe an improvement ...
TY - JOUR. T1 - Interleukin-10 delivery via mesenchymal stem cells. T2 - A novel gene therapy approach to prevent lung ischemia-reperfusion injury. AU - Manning, Eddie. AU - Pham, Si. AU - Li, Sen. AU - Vazquez-Padron, Roberto I. AU - Mathew, James. AU - Ruiz, Phillip. AU - Salgar, Shashikumar K.. PY - 2010/6/1. Y1 - 2010/6/1. N2 - Ischemia-reperfusion (IR) injury is an important cause of primary graft failure in lung transplantation. In this study, viral interleukin-10 (vIL-10)-engineered mesenchymal stem cells (MSCs) were tested for their ability to prevent lung IR injury. Bone marrow-derived MSCs were transduced with rvIL-10-retrovirus. After 120min of warm left lung ischemia, rats received ∼15×106 vIL-10-engineered MSCs (MSC-vIL-10), empty vector-engineered MSCs (MSC-vec), or saline intravenously. Mean blood oxygenation (PaO2/FiO2 ratio, mmHg) was ...
TY - JOUR. T1 - In vitro chondrogenesis of bone marrow-derived mesenchymal stem cells in a photopolymerizing hydrogel. AU - Williams, Christopher G.. AU - Kim, Tae Kyun. AU - Taboas, Anya. AU - Malik, Athar. AU - Manson, Paul. AU - Elisseeff, Jennifer Hartt. PY - 2003/8. Y1 - 2003/8. N2 - Mesenchymal stem cells (MSCS) from skeletally mature goats were encapsulated in a photopolymerizing poly(ethylene glycol)-based hydrogel and cultured with or without transforming growth factor β1 (TGF) to study the potential for chondrogenesis in a hydrogel scaffold system amenable to minimally invasive implantation. Chondrogenic differentiation was evaluated by histological, biochemical, and RNA analyses for the expression of cartilage extracellular matrix components. The two control groups studied were MSCs cultured in monolayer and MSCs encapsulated in ...
TY - JOUR. T1 - Cross-linked iron oxide nanoparticles for therapeutic engineering and in vivo monitoring of mesenchymal stem cells in cerebral ischemia model. AU - Park, Ji Won. AU - Ku, Sook Hee. AU - Moon, Hyung Ho. AU - Lee, Minhyung. AU - Choi, Donghoon. AU - Yang, Jaemoon. AU - Huh, Yong Min. AU - Jeong, Ji Hoon. AU - Park, Tae Gwan. AU - Mok, Hyejung. AU - Kim, Sun Hwa. PY - 2014/1/1. Y1 - 2014/1/1. N2 - Poly(ethylene glycol)-coated cross-linked iron oxide nanoparticles (PCIONs) are developed for therapeutic engineering of mesenchymal stem cells (MSCs) and their monitoring via magnetic resonance (MR) imaging at a time. PCIONs successfully combine with plasmid DNA (pDNA) via ionic interaction. Accordingly, PCION/pDNA complexes mediate superior translocations of vascular endothelial growth factor (VEGF) pDNA into intracellular regions of MSCs under ...
The immunomodulatory potential of cell therapies using adipose-derived stem cells (ASCs) and bone marrow-derived mesenchymal stem cells (BM-MSCs) has been studied in vascularized composite allotransplantation (VCA). Most cell therapy-based experimental and clinical protocols integrate some degree of recipient conditioning/induction with antibodies or other immunosuppressive agents. We investigated the susceptibility of ASCs and BM-MSCs to anti-lymphocyte serum (ALS) and tacrolimus. Rat ASCs and BM-MSCs were exposed to varying concentrations of tacrolimus and ALS in vitro. Serum from ALS-treated animals was added to cell cultures. Viability, susceptibility, and cytotoxicity parameters were evaluated. ALS inhibited ASC and BM-MSC viability and susceptibility in vitro in a dose-dependent manner. ASCs were more susceptible to ...
Multipotent adult progenitor cells are a recently described population of stem cells derived from the bone marrow stroma. multipotent adult progenitor cells along mesodermal lineages and exhibited the enhanced expression of alkaline phosphatase and production of calcium-containing mineral debris by multipotent adult progenitor cells, necessary precursors for osteogenesis. In combination with a demineralized bone matrix scaffold, multipotent adult progenitor cells exhibited enhanced revascularization and new bone formation in vivo in an orthotopic defect model when compared to mesenchymal stem cells on demineralized bone matrix or demineralized bone matrixConly control groups. The potent combination of angiogenic and osteogenic properties provided by ...
OBJECTIVE-Mesenchymal stem cells (MSCs) have been reported to secrete various cytokines that exhibit angiogenic and neurosupportive effects. This study was conducted to investigate the effects of MSC transplantation on diabetic polyneuropathy (DPN) in rats.. RESEARCH DESIGN AND METHODS-MSCs were isolated from bone marrow of adult rats and transplanted into hind limb skeletal muscles of rats with an 8-week duration of streptozotocin (STZ)-induced diabetes or age-matched normal rats by unilateral intramuscular injection. Four weeks after transplantation, vascular endothelial growth factor (VEGF) and basic fibroblast growth factor (bFGF) productions in transplanted sites, current perception threshold, nerve conduction velocity (NCV), sciatic nerve blood flow (SNBF), capillary number-to-muscle fiber ratio in soleus muscles, and sural nerve morphometry were evaluated.. RESULTS-VEGF and bFGF mRNA expression were ...
Fahmy, S. R., A. M. Soliman, M. E. Ansary, S. A. Elhamid, and H. Mohsen, Therapeutic efficacy of human umbilical cord mesenchymal stem cellstransplantation against renal ischemia/reperfusion injury in rats, Tissue & Cell, vol. 49, issue 3, pp. 369-375, 2017 ...
Zhang, Z.-Y., Chong, M.S.K., Teoh, S.-H., Schantz, J.T., Fisk, N.M., Choolani, M.A., Chan, J. (2009). Superior osteogenic capacity for bone tissue engineering of fetal compared with perinatal and adult mesenchymal stem cells. Stem Cells 27 (1) : 126-137. [email protected] Repository. https://doi.org/10.1634/stemcells.2008- ...
Human mesenchymal stem cells (MSCs) are multipotent and exert anti-inflammatory effects, but the underlying mechanism remains to be elucidated. In the current study, we investigated the regulatory mechanism of regulatory T cell (Treg) induction through the growth factors released by human MSCs. Human naive CD4+ T cells were stimulated with anti-CD3/28 Abs and cocultured with human MSC culture supernatant for 48 h. The proliferation and cytokine production of CD4+ T cells and surface molecule expression on CD4+ T cells were evaluated. The proliferation of anti-CD3/28 Abs-stimulated CD4+ T cells was suppressed by the addition of human MSC culture supernatant; in addition, the production of IL-10 and IL-4 increased. The human MSC culture supernatant induced CD4+FOXP3+ Tregs that expressed CD25, ...
Preterm newborns are at high risk of developing neurodevelopmental deficits caused by neuroinflammation leading to perinatal brain injury. Human Whartons jelly mesenchymal stem cells (hWJ-MSC) derived from the umbilical cord have been suggested to reduce neuroinflammation, in part through the release of extracellular vesicle-like exosomes. Here, we studied whether exosomes derived from hWJ-MSC have anti-inflammatory effects on microglia-mediated neuroinflammation in perinatal brain injury. Using ultracentrifugation, we isolated exosomes from hWJ-MSC culture supernatants. In an in vitro model of neuroinflammation, we stimulated immortalized BV-2 microglia and primary mixed glial cells with lipopolysaccharide (LPS) in the presence or absence of exosomes. In vivo, we introduced brain damage in 3-day-old rat pups and treated them intranasally with hWJ-MSC-derived exosomes. hWJ-MSC-derived ...
Dr. Cohens study utilizes the CTL based in the Case Western Reserve University School of Medicine NCRM, which is the only academic cleanroom facility for cellular therapy procedures in Northeast Ohio. The role of the CTL is to culture expand or "manufacture" the human MSCs for reinfusion into study subjects.. This study requires a specialized Class 10,000 cleanroom environment and uniquely qualified personnel because the study subjects marrow cells need to be grown ex vivo (outside of the body) for approximately a month to achieve an adequate number of MSCs. Once the desired dose is obtained, the cells are tested extensively to confirm they are viable and not contaminated to ensure safety, then cryopreserved. At a later date, they are transported to Mellen Center, carefully thawed, and infused intravenously. This type of cell manipulation is performed under the authority of the US Food and Drug ...
Mesenchymal stem cells (MSCs) are immunosuppressive, but we lack an understanding of how these adult stem cells are in turn affected by immune cells and the surrounding tissue environment. As MSCs have stromal functions and exhibit great plasticity, the influence of an inflamed microenvironment on their responses is important to determine. MSCs downregulate microglial inflammatory responses, and here we describe the mutual effects of coculturing mouse bone marrow MSCs with BV2 microglia in a lipopolysaccharide (LPS) inflammatory paradigm. Mouse MSCs were cultured from femoral and tibial bone marrow aspirates and characterized. MSCs were cocultured with BV2 microglia at four seeding-density ratios (1:0.2, 1:0.1, 1:0.02, and 1:0.01 (BV2/MSC)), and stimulated with 1 μg/ml LPS. In certain assays, MSCs were separated from BV2 ...
Multipotent mesenchymal stromal cells (MSCs) have potential therapeutic benefit for the treatment of neurological diseases and injury. MSCs interact with and alter brain parenchymal cells by direct cell-cell communication and/or by indirect secretion of factors and thereby promote functional recover …
TY - JOUR. T1 - Overexpression of human arginine decarboxylase rescues human mesenchymal stem cells against H2O2 toxicity through cell survival protein activation. AU - Seo, Su Kyoung. AU - Yang, Wonsuk. AU - Park, Yu Mi. AU - Lee, Won Taek. AU - Park, Kyung Ah. AU - Lee, Jong Eun. PY - 2013/3/1. Y1 - 2013/3/1. N2 - In this study, we explored the potentiality of human arginine decarboxylase (ADC) to enhance the survival of mesenchymal stem cells (MSCs) against unfavorable milieu of host tissues as the low survival of MSCs is the issue in cell transplantation therapy. To address this, human MSCs overexpressing human ADC were treated with H2O2 and the resultant intracellular events were examined. First, we examined whether human ADC is overexpressed in human MSCs. Then, we investigated cell ...
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OBJECTIVE To investigate the feasibility of employing double transplantations of autologous bone marrow mesenchymal stem cells (BMSC) and umbilical cord mesenchymal stem cells (UMSC) in the treatment of progressive muscular dystrophy (PMD). METHODS A total of 82 cases were treated by the double transplantations of BMSC and CB-MSC. They were diagnosed by clinical manifestations, CK, LDH, genetic analysis, electromyography, MRI and pathologic examination of biopsied muscle specimens from July 2007 to July 2008. Control group was self-made at before and after treatment and cases were followed up for 3 - 12 months. treatment method: Eighty-two patients underwent the double transplantations of bone mesenchymal stem cell (BMSC) and human umbilical cord blood MSC (CB-MSC). (1) ...
We have shown that undifferentiated embryonic stem cells do not express full-length MUC1. Rather, they express a low molecular weight cleavage product, MUC1*, which we previously demonstrated has growth factor receptor-like activity on tumor cells. NM23, which was shown to be an activating ligand of MUC1* on cancer cells, co-localizes with MUC1* on pluripotent cells. Unexpectedly, we found that newly differentiated cells no longer express cleaved MUC1* or its ligand, NM23. Newly differentiated stem cells present full-length MUC1. Transition zones between undifferentiated and differentiating cultured stem cells can be found that continue to express OCT4, while also expressing uncleaved, full-length MUC1, which appears to be a ...
There has been an explosion of research publications in the field of mesenchymal stem cells (MSCs) in the past 10 yr. Many researchers have sought to exploit their potential as a source of reparative cells for clinical use in a variety of contexts [1]. Adult stem cells are multipotent, undifferentiated, self-renewing capable of healing and regeneration of injured tissues [2-4]. Adult MSCs were initially identified in bone marrow (BM) and since then have been isolated and characterized from different human and animal adult tissues, peripheral blood, adipose, muscle, skin, dental pulp and other tissues [5-10].. Adult stem cells can be conveniently sampled from an extensive array of sources bypassing the ethical controversy associated with the embryonic stem ...
Simply put, adult stem cells, when taken as a whole, have the potential to grow into any of the bodys more than 200 cell types.. Adult Stem Cells are Ethical. Because adult stem cells are derived from adult tissues, there are no ethical dilemmas related to this type of therapy. Furthermore, research continues to show adult stem cells have far greater potential than embryonic stem cells to treat a variety of disease and conditions, including cancer, heart disease and neurodegenerative diseases.. It is only through continued research and clinical trials in patients that scientists and doctors will identify the different applications for adult stem cell therapy.. Adult Stem ...
Human being mesenchymal stromal cells (MSC) possess immunosuppressive and antimicrobial results that are partly mediated by the tryptophan-catabolizing enzyme indoleamine-2,3-dioxygenase (IDO). in the treatment of GvHD in transplant individuals, we recommend that individuals planned for MSC therapy should go through comprehensive evaluation for an energetic CMV disease and get CMV-directed antiviral therapy prior to the administration of MSC. 1. Intro Human being multipotent mesenchymal stromal cells (MSC), known for their multilineage difference potential, have pleiotropic immunosuppressive features that are mediated by phrase of the tryptophan-catabolizing enzyme indoleamine-2 partially,3-dioxygenase (IDO) [1C4]. Upon arousal with inflammatory cytokines, MSC show broad-spectrum antimicrobial effector features aimed against BMS-536924 different relevant pathogens medically, and these results are reliant on ...
Mesenchymal stem cells (MSCs) are multi-potent non-hematopoietic progenitor cells possessing an immune-regulatory function, with suppression of proliferation of activated lymphocytes. In this study, adult living donor kidney transplantation (LDKT) recipients were given MSCs derived from the donor bone marrow to evaluate the safety and the feasibility of immunological changes related to the intra-osseous injection of MSC into the bone marrow. MSCs were derived from negative HLA cross-match donors. Donor bone marrow was harvested 5 weeks prior to KT. At the time of transplantation, 1 x 106 cell/kg of donor MSC was directly injected into the bone marrow of the recipients right iliac bone. Patients clinical outcomes, presence of mixed chimerism by short tandem repeat polymerase chain reaction, analysis of plasma FoxP3 mRNA and cytokine level, ...
Purpose : To investigate if topically applied mesenchymal stem cell (MSC)-derived extracellular vesicles (EVs) promote corneal wound repair in a rat alkali burn model Methods : EVs were isolated from media conditioned by human bone marrow-derived MSCs (n=4) using a series of centrifugation, filtration and ultracentrifugation steps followed by transmission electron microscopy (TEM) and nanoparticle tracking analysis (NTA; NanoSight) quality control. Pro-healing effects of hMSC-derived EVs (hMSC-EV) were examined in vitro by cell migration and scratch wound healing assays using human corneal epithelial (HCE) and endothelial (HCEC) cell lines. Human cornea wound healing rate was also determined ex vivo using fluorescein penetration test. Rat corneal alkali burn injury was induced using 1M NaOH and injury was treated with or without 30mg of hMSC-EVs. Efficacy of treatment was monitored by biomicroscopy and ...
Adipose tissue-derived stem cells are a powerful tool for in vitro study of adult stem cell biology. So far, they have not been extensively used for gain or loss of function studies since they are resistant to most common transfection methods. Herein, we tested several classic transfection methods on human multipotent adipose tissue-derived stem (hMADS) cells. Our results showed that lipofectants and calcium phosphate were poorly efficient for transgene delivery in hMADS cells. In contrast, nucleofection, an electroporation-based method that is assumed to target plasmid DNA directly to the cell nucleus, led to a significant transient transgene expression in hMADS cells (up to 76% enhanced green fluorescent protein [EGFP]-positive cells were detected). ...
Objective: The hepatic integration of human adipose tissue derived mesenchymal stem cells (hAT-MSCs) in vivo with or without prior differentiation to hepatocyte-like cells in vitro was investigated.. Methods and results: Cells, isolated either from peritoneal or subcutaneous adipose tissue, expressed mesenchymal stem cell surface markers and featured multiple lineage differentiation. Under conditions favouring hepatocyte differentiation, hAT-MSCs gained hepatocytic functions in vitro including urea formation, glycogen synthesis, cytochrome P450 enzyme activity, and expression of hepatocyte-specific transcripts of carbamoylphosphate synthetase, albumin and cytochrome P450 type 3A4 (CYP3A4). Transgenic expression of green fluorescent protein emerged upon hepatocyte differentiation when driven by the hepatocyte-specific ...
Adult stem cell treatments have been used for many years to successfully treat leukemia and related bone/blood cancers utilizing bone marrow transplants.[49] The use of adult stem cells in research and therapy is not considered as controversial as the use of embryonic stem cells, because the production of adult stem cells does not require the destruction of an embryo. Early regenerative applications of adult stem cells has focused on intravenous delivery of blood progenitors known as Hematopetic Stem Cells (HSCs). CD34+ hematopoietic Stem Cells have been clinically applied to treat various diseases including spinal cord injury,[50] liver cirrhosis [51] and ...
Background: The aim was to test the hypothesis that the blood serum of rats subjected to recurrent airway obstructions mimicking obstructive sleep apnea (OSA) induces early activation of bone marrow-derived mesenchymal stem cells (MSC) and enhancement of endothelial wound healing. Methods: We studied 30 control rats and 30 rats subjected to recurrent obstructive apneas (60 per hour, lasting 15 s each, for 5 h). The migration induced in MSC by apneic serum was measured by transwell assays. MSC-endothelial adhesion induced by apneic serum was assessed by incubating fluorescent-labelled MSC on monolayers of cultured endothelial cells from rat aorta. A wound healing assay was used to investigate the effect of apneic serum on endothelial repair. Results: Apneic serum showed significant increase in chemotaxis in MSC when compared with control serum: the normalized chemotaxis indices were 2.20 +- 0.58 (m +- SE) and ...
Introduction: Rheumatoid arthritis (RA) is a T-cell-mediated systemic autoimmune disease, characterized by synovium inflammation and articular destruction. Bone marrow mesenchymal stem cells (MSCs) could be effective in the treatment of several autoimmune diseases. However, there has been thus far no report on umbilical cord (UC)-MSCs in the treatment of RA. Here, potential immunosuppressive effects of human UC-MSCs in RA were evaluated.. Methods: The effects of UC-MSCs on the responses of fibroblast-like synoviocytes (FLSs) and T cells in RA patients were explored. The possible molecular mechanism mediating this immunosuppressive effect of UC-MSCs was explored by addition of inhibitors to indoleamine 2,3-dioxygenase (IDO), Nitric oxide (NO), prostaglandin E2 (PGE2), transforming growth factor beta 1 (TGF-beta 1) and interleukin 10 (IL-10). The therapeutic effects of ...
Heng, B.C.,Phan, T.T.,Liu, H.,Ouyang, H.W.,Cao, T. (2006). Can the therapeutic advantages of allogenic umbilical cord blood-derived stem cells and autologous bone marrow-derived mesenchymal stem cells be combined and synergized?. ASAIO Journal 52 (6) : 611-613. [email protected] Repository. https://doi.org/10.1097/01.mat.0000235330.02549.78 ...
TY - JOUR. T1 - Cell-cell contact with proinflammatory macrophages enhances the immunotherapeutic effect of mesenchymal stem cells in two abortion models. AU - Li, Yanhong. AU - Zhang, Di. AU - Xu, Ling. AU - Dong, Lin. AU - Zheng, Ji. AU - Lin, Yikong. AU - Huang, Jiefang. AU - Zhang, Yanyun. AU - Tao, Yu. AU - Zang, Xingxing. AU - Li, Dajin. AU - Du, Meirong. PY - 2019/12/1. Y1 - 2019/12/1. N2 - Mesenchymal stem cells (MSCs), which are pluripotent cells with immunomodulatory properties, have been considered good candidates for the therapy of several immune disorders, such as inflammatory bowel diseases, concanavalin A-induced liver injury, and graft-versus-host disease. The embryo is a natural allograft to the maternal immune system. A successful pregnancy depends on the timely extinction ...
Sun Z, Kennedy KS, Tee BC, Damron JB, Allen MJ. 2014. Establish a critical-size mandibular defect model in growing pigs: characterization of spontaneous healing. J of Oral Maxillofacial Surgery, 72(9):1852-68. Patel A, Tee BC, Fields HW, Jones E, Chaudhry J, Sun Z. 2014. The accuracy and inaccuracy of using CBCT to diagnose small osseous defects at the mandibular condyle. American Journal of Orthodontics & Craniofacial Orthopedics, 145(2):143-56.. Sun Z, Tee BC, Kennedy KS, Kennedy PM, Kim D-G, Mallery SR, Fields HW. 2013. Scaffold-Based Delivery of Autologous Mesenchymal Stem Cells for Mandibular Distraction Osteogenesis: Preliminary Studies in a Porcine Model. PloS One. 8(9): e74672.. Sun Z, Herring SW, Tee BC, Gale J. 2013. Alveolar ridge reduction after tooth extraction in adolescent pigs. Archives of Oral Biology. 58(7): 813-825.. Yan B, Sun Z, Fields HW, Wang L. 2013. Maxillary canine impaction increased root resorption risk of ...
BACKGROUND: The introduction of specific BCR-ABL inhibitors in chronic myelogenous leukemia therapy has entirely mutated the prognosis of this hematologic cancer from being a fatal disorder to becoming a chronic disease. Due to the probable long lasting treatment with tyrosine-kinase inhibitors (TKIs), the knowledge of their effects on normal cells is of pivotal importance.. DESIGN AND METHODS:. We investigated the effects of dasatinib treatment on human bone marrow-derived mesenchymal stromal cells (MSCs).. RESULTS:. Our findings demonstrate, for the first time, that dasatinib induces MSCs adipocytic differentiation. Particularly, when the TKI is added to the medium inducing osteogenic differentiation, a high MSCs percentage acquires adipocytic morphology and overexpresses adipocytic specific genes, including PPARγ, CEBPα, LPL and SREBP1c. Dasatinib also inhibits the activity of alkaline phosphatase, an osteogenic marker, ...
Bone marrow derived human mesenchymal stem cells (hMSCs) show promising potential in regeneration of defective tissue. Recently, gene silencing strategies using microRNAs (miR) emerged with the aim to expand the therapeutic potential of hMSCs. However, researchers are still searching for effective miR delivery methods for clinical applications. Therefore, we aimed to develop a technique to efficiently deliver miR into hMSCs with the help of a magnetic non-viral vector based on cationic polymer polyethylenimine (PEI) bound to iron oxide magnetic nanoparticles (MNP). We tested different magnetic complex compositions and determined uptake efficiency and cytotoxicity by flow cytometry. Additionally, we monitored the release, processing and functionality of delivered miR-335 with confocal laser scanning microscopy, real-time PCR and live cell imaging, respectively. On this basis, we established parameters for construction of ...
The development, maintenance and repair of the skeletal system are dependent on the differentiation of both chondrocytes and osteoblasts from their common progenitor, the mesenchymal stem cell (MSC). The A2B adenosine receptor (A2BAR) is a G-protein-coupled receptor that signals by increasing cAMP and/or activating phospholipase C signaling. Considering the published roles of cAMP on MSC differentiation, and our finding that the expression of the A2BAR is induced following injury, we hypothesized that ablation or activation of the A2BAR impacts the differentiation of osteoblasts and chondrocytes and that this would manifest as changes in skeletal development and bone fracture repair. Activation of the A2BAR increased the differentiation of bone marrow-derived MSCs to osteoblasts by increasing mRNA expression of the transcription factors runt-related transcription factor 2 (Runx2) and Sp7 transcription factor (Osterix), which are essential ...
Results Culture-expanded amnion-derived adherent cells expressed mesenchymal stem cell markers and HLA-ABC molecules and could differentiate into osteoblasts, adipocytes and chondrocyte-like cells, indicating that the cells have the characteristics of mesenchymal stem cells. The Lin-CD34+ cells purified from the frozen umbilical cord blood were strongly positive for HLA-ABC, and contained a large number of hematopoietic stem cells. When the Lin-CD34+ cells were cultured on the autologous (MHC-matched) or MHC-mismatched amnion-derived adherent cells in short-term assays (hematopoietic stem ...
The purpose of this study was to track the differentiation profile and stress response of human bone marrow derived mesenchymal stem cells (hMSCs) undergoing osteogenic differentiation during exposure to a 20 mV/cm, 60 kHz electric field. Morphological and biochemical changes were imaged using endogenous two-photon excited fluorescence (TPEF) and quantitatively assessed through eccentricity calcul... read moreations and extraction of the redox ratio from NADH, FAD and lipofuscin contributions. Real time reverse transcriptase-polymerase chain reactions (RT-PCR) were used to track osteogenic differentiation markers, namely alkaline phosphatase (ALP) and collagen type 1 (col1), and stress response markers, such as heat shock protein 27 (hsp27) and heat shock protein 70 (hsp70) Comparisons of collagen deposition between the stimulated hMSCs and controls were examined through second harmonic generation (SHG) imaging. read less. ...
Purpose : Mesenchymal stem cell (MSC)-based therapy is under investigation as a treatment strategy to promote tissue repair and to modulate the immune system in immune mediated disorders. MSCs have been isolated from many tissues most commonly from the bone marrow or fat. Given the cumbersome procedures for harvesting bone marrow (BM), and to a lesser extent fat, there is a need to look for other tissues for MSC isolation. The International Society for Cellular Therapy (ISCT) has established three criteria for characterizing human MSCs: adherence to plastic, differentiation potential, and the presence or absence of certain cell surface markers. We evaluated whether or not corneal limbal stromal cells (CLSC) met ISCT criteria by comparing them to BM-MSCs. Methods : BM-MSCs were isolated from healthy human bone marrow donors. CLSC were isolated from human corneal limbus from ...
Background. Haploidentical hematopoietic stem-cell transplantation (haplo-HSCT) is associated with an increased risk of graft failure and severe graft-versus-host disease (GVHD). Marrow mesenchymal stromal cells (MSCs) have been shown to support in vivo normal hematopoiesis and to display potent immunosuppressive effects. We launched a multi-center clinical study to examine the safety and feasibility of co-transplantation of MSCs (from third party donors) and haploidentical HSCs into 35 children with severe aplastic anemia (SAA). Methods. A total of 35 children with SAA were enrolled in this multi-center study between January 2014 and December 2016. All patients met the criteria of HLA-mismatched with ⩾5/10 HLA-matched loci. The conditioning regimen for haploidentical hematopoietic stem cell transplantation ...
A number of current stem cell treatments already. their ability to make choices explains why they stay as stem cells in culture.Stap cells: research paper on stem cell breakthrough was partly falsified.This journal is a member of and subscribes to the principles of the Committee on Publication Ethics.In five pages this research paper considers embryonic stem cells and current research in this.Humans Clinical outcome after stem cell mobilization with granulocyte-colony-stimulating factor after acute ST-elevation myocardial infarction: 5-year results of the STEMMI trial Bookmark Download by.This walkthrough for stem cells are you need your access is central storage warehouse co.Humans The Clinical Impact of ...
The possibility for isolating bovine mesenchymal multipotent cells (MSCs) from fetal adnexa is an interesting prospect because of the potential for these cells to be used for biotechnological applications. Bone marrow and adipose tissue are the most common sources of MSCs derived from adult animals. However, little knowledge exists about the characteristics of these progenitors cells in the bovine species. Traditionally most cell cultures are developed in two dimensional (2D) environments. In mammalian tissue, cells connect not only to each other, but also support structures called the extracellular matrix (ECM). The three-dimensional (3D) cultures may play a potential role in cell biotechnology, especially in tissue therapy. In this study, bovine-derived umbilical cord Whartons jelly (UC-WJ) ...
The aim of this study is to conduct in vivo, noninvasive magnetic resonance imaging of labeled rat bone mesenchymal stem cells (BMSCs) as they home into the site of injured common carotid artery following allograft transplantation. Our study was approved by the Institutional Committee on Animal Research. Purified rat BMSCs were dual labeled with superparamagnetic iron oxide (SPIO) particle and fluorescent DiI dye, and subsequently transplanted into recipient rats injured in the left common carotid arteries. Immediately before and 3 hr, 3, 7 and 12 days after transplantation, the labeled cells were monitored in vivo using a 7T micromagnetic resonance imaging (7T micro-MRI) scanner. The signal-to-noise ratios (SNRs) at the injured sites were corroborated with histological examination using Prussian blue staining and fluorescent imaging. Rat BMSCs were labeled with SPIO and DiI ...
Pak J. et al, 2017.) (2). 1. REF: Osteoarthritis Research Society International. (2017). OARSI Guidelines. [online] Available at: https://www.oarsi.org/education/oarsi-guidelines [Accessed 22 Sep. 2017].. 2. REF: Pak, J., Lee, J., Park, K., Park, M., Kang, L. and Lee, S. (2017). Current use of autologous adipose tissue-derived stromal vascular fraction cells for orthopedic applications. Journal of Biomedical Science, 24(1).. Tags: Macquarie Stem Cells, Dr. Bright, Osteoarthritis Treatment, Dr. Ralph Bright, Stem Cell Therapy, Stem Cell Treatment, Stem Cells for Arthritis. ...
Skeletal muscle plays an important role in the bodys physiology but there are still no effective treatments for volumetric muscle loss (VML) resulting from severe traumatic injury or tumor excision. Recent studies show that a tissue engineering strategy using a compound containing mesenchymal stem cells (MSCs) and decellularized extracellular matrix (ECM) scaffold generates significant regenerative effects on VML injury, but the underlying mechanisms are not fully understood. The characteristics of human umbilical cord MSCs, including multiplication capacity and multidifferentiation ability, were determined. We constructed a compound containing MSCs and decellularized ECM scaffold which was used for tissue regeneration in a VML model. We found that MSCs and decellularized ECM scaffold generated synergistic effects on promoting skeletal muscle tissue regeneration. ...
TY - JOUR. T1 - Combination of three angiogenic growth factors has synergistic effects on sprouting of endothelial cell/mesenchymal stem cell-based spheroids in a 3D matrix. AU - Kim, Sook Kyoung. AU - Lee, Jaeyeon. AU - Song, Myeongjin. AU - Kim, Mirim. AU - Hwang, Soon Jung. AU - Jang, Hwanseok. AU - Park, Yongdoo. PY - 2015. Y1 - 2015. N2 - Combinations of angiogenic growth factors have been shown to have synergistic effects on angiogenesis and natural wound healing in various animal models. Each growth factor has unique roles during angiogenesis; vascular endothelial growth factor (VEGF) plays a key role during the initial step of angiogenesis, whereas PDGF functions in the maturation of blood vessels. We used a combination of three angiogenic growth factors to increase angiogenesis in vitro and in vivo. We chose VEGF as a basic factor and added platelet-derived growth factor (PDGF) and fibroblast growth factor (FGF) to induce ...
Focal adhesions (FAs) undergo maturation culminating in size and composition changes that modulate adhesion, cytoskeleton remodeling and differentiation. While it is well-recognized that stimuli for osteogenesis of mesenchymal stem cells (MSCs) drive FA maturation, actin organization, and stress-fiber polarization, the extent to which FA-mediated signals regulated by the FA protein composition specifies MSC commitment remains largely unknown. Here we demonstrate that, upon dexamethasone (osteogenic induction) treatment, guanine nucleotide exchange factor-H1 (GEF-H1) is significantly enriched in FAs. Perturbation of GEF-H1 inhibits FA formation, anisotropic stress-fiber orientation and MSC osteogenesis in an actomyosin contractility-independent manner. To determine the role of GEF-H1 in MSC osteogenesis, we explore the GEF-H1-modulated FA proteome that reveals non-muscle myosin-II heavy chain-B (NMIIB) as a target of GEF-H1 in FAs. Inhibition ...
Tissue engineering strategies have been developed to optimize osseointegration in dental implant surgery. One of the major problems is the non-homogeneous spatial cell distribution in the scaffold, as well as subsequent matrix production. Insufficient nutrient and oxygen supplies inside the scaffold are factors in this phenomenon. To mediate this gradient formation, we have implemented a perfusion culture method to seed human bone marrow mesenchymal stem cells (MSCs) into three-dimensional (3-D)-allogenic bone scaffolds in combination with a marine haemoglobin, HEMOXCell®, for oxygen delivery. Cell culture was performed under static and perfusion conditions, with standard and osteogenic media, with and without HEMOXCell®. The cell seeding efficiency, as well as MSC/scaffold cytocompatibly were assessed using viability and proliferation assays. Scaffolds ...
The thesis observes that, unlike international commentaries which largely focus on embryonic stem cell treatments, in India it is adult and cord blood stem cells which are dominant in research and clinical settings. Expectations are configured on the basis that stem cells have the potential to: solve the problem of organ shortage; help patients with ailments; provide affordable health care; and establish India as a global player. The creation of expectations is ethically problematic given the potential health risks and economic exploitation of both native and international patients. However, the ethically contested activities are justified by clinicians on the basis that the Helsinki Declaration allows to use an experimental therapy; there are many desperate patients demanding these treatments; and adult stem cells are safe ...
Recently, therapy with bone marrow mesenchymal stem cells (BMMSCs) has been attempted to relieve rheumatoid arthritis (RA) and reconstruct cartilage injury. However, treatment has been unsuccessful in complete prevention of persistent cartilage destruction and resulted in inferior outcomes of cartilage regeneration. Scaffolds are an important construct in the field of cartilage tissue engineering, but their role in arthritis treatment has not yet been fully examined. Here, we transplanted two types of scaffold-assisted BMMSCs: fibrin gel- and poly(l-lactide-co-glycolide)−poly(ethylene glycol)−poly(l-lactide-co-glycolide) (PLGA−PEG−PLGA) hydrogel-assisted BMMSCs referred as FGB and HGB groups, respectively, into subchondral defects for the treatment of antigen-induced arthritis. The administration of exogenous BMMSCs ameliorated joint swelling and decreased both joint surface temperature and inflammatory cytokine levels in both groups. Immune ...
There are two basic stem cell types. The first is called unlimited stem cells (also known as embryonic stem cells). These can turn into any kind of cell, while the second type is termed limited stem cells (also known as adult stem cells).. With the unlimited type of stem cells, the cells have the potential to become any human cell type. They can be replicated outside the body and have applications for many human diseases.. Limited stem cells, though, do not have the same limitless potential and cannot be replicated outside the body. They need to be either frozen or immediately transplanted into the body.. R3 ...
Endothelial progenitor cells (EPCs) were first isolated as CD34+ mononuclear cells (MNCs) from adult peripheral blood.1,2 Tissue ischemia mobilizes EPCs from bone marrow to peripheral blood, and mobilized EPCs home specifically to sites of nascent neovascularization and differentiate into mature endothelial cells (ECs).3 The demonstrated role of EPCs in the physiological response to ischemia has led to the development of strategies of cell therapy for neovascularization in ischemic diseases. Intravenous transplantation of cultured human EPCs enhances neovascularization and improves limb salvage in nude mice with hindlimb ischemia.4 A similar strategy applied in a model of myocardial ischemia in the nude rat demonstrated that transplanted human EPCs incorporated into rat myocardial neovascularization, differentiated into mature ECs in ischemic myocardium, enhanced ...
Three patients went blind after they received eye injections of stem cells, The New England Journal of Medicine reported. The patients were seen by some of the reports co-investigators, led by Ajay E Kuriyan, MD. The experimental treatments were performed at a stem-cell clinic and involved stem cells derived from the patients own abdominal fat. Patients who received the treatments were in the early stages of age-related macular degeneration. "The patients severe visual loss after the injection was associated with ocular hypertension, hemorrhagic retinopathy, vitreous hemorrhage, combined traction, and rhegmatogenous retinal detachment, or lens dislocation," the investigators wrote. "After 1 year, the patients visual acuity ranged from 20/200 to no light perception." The company sponsoring the treatments was U.S. Stem Cell, Inc. Authors said ...
Extracellular Matrix Protein Laminin Enhances Mesenchymal Stem Cell (MSC) Paracrine Function through αvβ3/CD61 Integrin to Reduce Cardiomyocyte Apoptosis Researchers found that placenta-derived multipotent cells (PDMCs) significantly reduced cardiomyocyte apoptosis and reactive oxygen species production through the paracrine factors GRO-α, HGF and IL-8. The enhancement of PDMC paracrine function by laminin was mediated through αvβ3 integrin, with involvement of the signaling pathways of JNK, for GRO-α and IL-8 secretion, and PI3K/AKT, for HGF secretion. [J Cell Mol Med] Full Article Transplantation of Human Bone Marrow Mesenchymal Stromal Cells Reduces Liver Fibrosis More Effectively than Whartons Jelly Mesenchymal Stromal Cells The authors compared the relative therapeutic ...
Bone marrow-derived mesenchymal stem cells (MSCs) reduce acute lung injury in animals challenged by bleomycin or bacterial lipopolysaccaride. It is not known, however, whether MSCs protect from ventilator-induced lung injury (VILI). This study investigated whether MSCs have a potential role in preventing or modulating VILI in healthy rats subjected to high-volume ventilation. 24 Sprague-Dawley rats (250-300 g) were subjected to high-volume mechanical ventilation (25 mL.kg(-1)). MSCs (5 x 10(6)) were intravenously or intratracheally administered (n=8 each) 30 min before starting over-ventilation and eight rats were MSC-untreated. Spontaneously breathing anesthetised rats (n=8) served as controls. After 3 h of over-ventilation or control the animals were sacrificed and lung tissue and bronchoalveolar lavage fluid (BALF) were sampled for further analysis. When compared with controls, MSC-untreated over-ventilated rats exhibited typical VILI ...
More frequent utilization of non-heart-beating donor (NHBD) organs for lung transplantation has the potential to relieve the shortage of donor organs. In particular with respect to uncontrolled NHBD, concerns exist regarding the risk of ischaemia/reperfusion (IR) injury-related graft damage or dysfunction. Due to their immunomodulating and tissue-remodelling properties, bone-marrow-derived mesenchymal stem cells (MSCs) have been suspected of playing a beneficial role regarding short- and long-term survival and function of the allograft. Thus, MSC administration might represent a promising pretreatment strategy for NHBD organs. To study the initial effects of warm ischaemia and MSC application, a large animal lung transplantation model was generated, and the structural organ composition of the transplanted lungs was analysed stereologically with particular respect to the blood-gas barrier and the surfactant ...
By Dr. Kieran OSullivan, University of Limerick, Ireland. And Prof. Peter OSullivan, Curtin University, Australia. The 14-time Grand Slam winner, Rafael Nadals recent struggles to participate at the highest level due to ongoing low back pain (LBP) once again brings the issue of novel therapies offering tantalizing cures to the fore; he is undergoing stem cell therapy (SCT). So it is timely to explore: i) what this therapy potentially offers, ii) the quality of the supporting evidence, iii) its comparison to other needle-based therapies, and iv) what the use of several novel needle-based therapies in the last decade (e.g. stem cell therapy, platelet-rich plasma, dry needling) reveal about attitudes to management of pain in sporting populations.. What is stem cell therapy and why consider it?. Stem cell therapy (SCT) has opened up exciting avenues in health research across ...
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Our previous reports indicated that (+)-cholesten-3-one induces osteogenic differentiation of bone marrow mesenchymal stem cells (MSCs) by activating vitamin D receptor (VDR). However, whether and how miRNAs modulate osteogenic differentiation induced by (+)-cholesten-3-one have not been explored. In this study, miRNA array profiling and further validation by quantitative real-time PCR revealed that miR-351 was downregulated during (+)-cholesten-3-one-induced osteogenic differentiation of MSCs. Overexpression of miR-351 by miR-351 precursor transfection markedly inhibited the expression of osteoblast-specific genes, such as alkaline phosphatase (ALP), collagen type II, osteopontin (OPN), and runt-related transcription factor 2 (RUNX2), which consequently decreased a number of calcium mineralized nodules ...
Lymph node stromal cells are essential to the structure and function of the lymph node. There are a number of different types of lymph node stromal cells which have a number of functions including: creating a tissue scaffold within lymph nodes for the support of hematopoietic cells; the release of small molecules that are chemical messengers that facilitate interactions between hematopoietic cells; the facilitation of the migration of hematopoietic cells; the presentation of antigens to immune cells at the initiation of the adaptive immune system; and the homeostasis of lymphocyte numbers. Stromal cells originate from multipotent mesenchymal stem ...

Umbilical cord-derived mesenchymal stem cells: strategies, challenges, and potential for cutaneous regenerationUmbilical cord-derived mesenchymal stem cells: strategies, challenges, and potential for cutaneous regeneration

Autologous mesenchymal stem cell transplantation induce VEGF and neovascularization in ischemic myocardium. Regul Pept 2004; ... Mesenchymal stem/progenitor cells in human umbilical cord blood as support for ex vivo expansion of CD34(+) hematopoietic stem ... Mesenchymal stem cells in the Whartons jelly of the human umbilical cord. Stem Cells 2004; 22(7): 1330-1337 doi: 10.1634/ ... Stem cell characteristics of amniotic epithelial cells. Stem Cells 2005; 23(10): 1549-1559 doi: 10.1634/stemcells.2004-0357 ...
more infohttp://journal.hep.com.cn/fmd/EN/10.1007/s11684-012-0175-9

Hypomethylating agents are effective in myelodysplastic syndrome | Cancer BiologyHypomethylating agents are effective in myelodysplastic syndrome | Cancer Biology

MDS develops as the consequence of a series of genetic changes in a hematopoietic stem cell. These changes alter normal ... disease are considered for front-line chemotherapy approaches followed perhaps by allogeneic stem cell transplantation. ... Non-proliferating cells are relatively insensitive to decitabine.. Biological mechanism of action of azacitidine (AZA) and ... which prevents epithelial mesenchymal transition. Epigenomic abnormalities silence the expression of these genes; ...
more infohttps://blogs.shu.edu/cancer/2016/09/28/hypomethylating-agents-are-effective-in-myelodysplastic-syndrome/

Umbilical Cord-Derived Mesenchymal Stem Cells for Hematopoietic Stem Cell TransplantationUmbilical Cord-Derived Mesenchymal Stem Cells for Hematopoietic Stem Cell Transplantation

... Yu-Hua Chao,1,2 Han-Ping Wu,3 Chin- ... "Mesenchymal stem cells are present in peripheral blood and can engraft after allogeneic hematopoietic stem cell transplantation ... "Transplantation of mesenchymal stem cells to enhance engraftment of hematopoietic stem cells," Leukemia, vol. 21, no. 8, pp. ... "Umbilical cord mesenchymal stem cells: adjuvants for human cell transplantation," Biology of Blood and Marrow Transplantation, ...
more infohttps://www.hindawi.com/journals/bmri/2012/759503/

Co-transplantation of Hematopoietic Stem Cells and Cxcr4 Gene-Transduced Mesenchymal Stem Cells Promotes Hematopoiesis |...Co-transplantation of Hematopoietic Stem Cells and Cxcr4 Gene-Transduced Mesenchymal Stem Cells Promotes Hematopoiesis |...

Co-transplantation of MSCs and hematopoietic stem cells (HSCs) promotes successful engraftment and improves... ... Mesenchymal stem cells (MSCs) are a promising candidate for cellular therapies. ... Karp, J. M., & Leng Teo, G. S. (2009). Mesenchymal stem cell homing: The devil is in the details. Cell Stem Cell, 4, 206-216. ... Co-transplantation of Hematopoietic Stem Cells and Cxcr4 Gene-Transduced Mesenchymal Stem Cells Promotes Hematopoiesis. ...
more infohttps://link.springer.com/article/10.1007%2Fs12013-014-0381-y

Cotransplantation of bone marrow-derived mesenchymal stem cells in haploidentical hematopoietic stem cell transplantation in...Cotransplantation of bone marrow-derived mesenchymal stem cells in haploidentical hematopoietic stem cell transplantation in...

Mesenchymal stem cells (MSCs) have been shown to support hematopoiesis in vivo and to display potent immunosuppressive effects ... Haploidentical hematopoietic stem cell transplantation (haplo-HSCT) for severe aplastic anemia (SAA) is mainly limited by the ... Cotransplantation of bone marrow-derived mesenchymal stem cells in haploidentical hematopoietic stem cell transplantation in ... Haploidentical hematopoietic stem cell transplantation (haplo-HSCT) for severe aplastic anemia (SAA) is mainly limited by the ...
more infohttps://insights.ovid.com/bone/201705000/00002605-201705000-00008

Paper: Donor-Derived Marrow Mesenchymal Stem Cells Co-Transplantation Following Haploidentical Hematopoietic Stem Cell...Paper: Donor-Derived Marrow Mesenchymal Stem Cells Co-Transplantation Following Haploidentical Hematopoietic Stem Cell...

4482 Donor-Derived Marrow Mesenchymal Stem Cells Co-Transplantation Following Haploidentical Hematopoietic Stem Cell ... Haploidentical hematopoietic stem-cell transplantation (haplo-HSCT) is associated with an increased risk of graft failure and ... The conditioning regimen for haploidentical hematopoietic stem cell transplantation consisted of busulfan (Bu), ... MSCs (1×106cells/kg) were administered by venous infusion within 6 h proceeding stem cell infusion on day 01 then on day +14 ( ...
more infohttps://ash.confex.com/ash/2017/webprogram/Paper103945.html

Frontiers | Mesenchymal Stem Cells May Ameliorate Nephrotic Syndrome Post-Allogeneic Hematopoietic Stem Cell Transplantation...Frontiers | Mesenchymal Stem Cells May Ameliorate Nephrotic Syndrome Post-Allogeneic Hematopoietic Stem Cell Transplantation...

... after allogeneic hematopoietic stem cell transplantation (allo-HSCT) is an uncommon complication with unclear etiology and ... after allogeneic hematopoietic stem cell transplantation (allo-HSCT) is an uncommon complication with unclear etiology and ... It may be an immune disorder involving immune complex deposition, B cells, regulatory T cells (Tregs) and Th1 cytokines and be ... It may be an immune disorder involving immune complex deposition, B cells, regulatory T cells (Tregs) and Th1 cytokines and be ...
more infohttps://www.frontiersin.org/articles/10.3389/fimmu.2017.00962/full

Mesenchymal stem cells enhance the induction of mixed chimerism and tolerance to rat hind-limb allografts after bone marrow...Mesenchymal stem cells enhance the induction of mixed chimerism and tolerance to rat hind-limb allografts after bone marrow...

CONCLUSION This study indicates a potential use of MSCs for induction of stable and high level mixed hematopoietic chimerism ... rats were used as cell or hind-limb donors and recipients, respectively. Recipients received a conditioning regimen consisting ... In this study, we investigated the potential of mesenchymal stem cells (MSCs) in the induction of stable and high level ... followed by an intraportal co-infusion of allogeneic MSCs and bone marrow cells (day 0); then performing hind-limb ...
more infohttps://www.semanticscholar.org/paper/Mesenchymal-stem-cells-enhance-the-induction-of-and-Pan-Zhao/4a9ea48f9625ea837dffce2924d3e5846d6b6690

Bone-marrow-derived stem cells - our key to longevity? | SpringerLinkBone-marrow-derived stem cells - our key to longevity? | SpringerLink

In this review we discuss current views of the BM stem ce ... primarily regarded as the source of hematopoietic stem cells. ... Role of mesenchymal stem cells in hematopoietic stem cell transplantation. Curr Opin Hematol 7: 358-363. ... Sarcoma derived from cultured mesenchymal stem cells. Stem Cells 25: 371-379. ... Derivation of human embryonic germ cells: an alternative source of pluripotent stem cells. Stem Cells 21: 598-609. ...
more infohttps://link.springer.com/article/10.1007%2FBF03195227

Effect of chemotherapy for acute myelogenous leukemia on hematopoietic and fibroblast marrow progenitors - Semantic ScholarEffect of chemotherapy for acute myelogenous leukemia on hematopoietic and fibroblast marrow progenitors - Semantic Scholar

... the question was raised to what extent hematopoietic and microenvironmental progenitor cells were involved in these patients. ... Seven samples failed to support any progenitor cell growth, seven had a significantly lower supportive activity as compared to ... The association of hematopoietic with microenvironmental damage might play a key role in the delayed hematopoietic regeneration ... Marrow hematopoietic progenitors were investigated by a short-term methylcellulose assay quantitating multipotent CFU-Mix, ...
more infohttps://www.semanticscholar.org/paper/Effect-of-chemotherapy-for-acute-myelogenous-on-and-Carlo-Stella-Tabilio/a7c3f5141c8b332dc9def2f857ae0f1e999541dd

IJMS | Free Full-Text | Characterization of Copy Number Variations Potential Role in Mareks DiseaseIJMS | Free Full-Text | Characterization of Copy Number Variation's Potential Role in Marek's Disease

Mesenchymal Stem Cell Derived Extracellular Vesicles: A Role in Hematopoietic Transplantation?. Next Article in Special Issue. ...
more infohttps://www.mdpi.com/1422-0067/18/5/1020

IJMS | Free Full-Text | Engineering Exosomes for Cancer TherapyIJMS | Free Full-Text | Engineering Exosomes for Cancer Therapy

Studies to date have employed viral and non-viral methods to engineer the parent cells to secrete modified exosomes, or ... The majority of studies have demonstrated promising results, with decreased tumor cell invasion, migration and proliferation, ... along with enhanced immune response, cell death, and sensitivity to chemotherapy observed. The studies outlined in this review ... Following secretion, exosomes are naturally taken up by cells, and capable of the stable transfer of drugs, therapeutic ...
more infohttp://www.mdpi.com/1422-0067/18/6/1122

nature.com searchnature.com search

The potential use of mesenchymal stem cells in hematopoietic stem cell transplantation *Eun-Jung Kim ... Rights & permissionsfor article The potential use of mesenchymal stem cells in hematopoietic stem cell transplantation . Opens ... Enhanced immunoregulation of mesenchymal stem cells by IL-10-producing type 1 regulatory T cells in collagen-induced arthritis ... Successful outcomes of second hematopoietic stem cell transplantation with total nodal irradiation and ATG conditioning for ...
more infohttp://www.nature.com/search?author=%22Seok-Goo+Cho%22&error=cookies_not_supported&code=17d31d27-3fc9-45d7-8f8a-bec3e1dc7308

Mesenchymal Stem Cells Combined With Cord Blood for Treatment of Graft Failure - Full Text View - ClinicalTrials.govMesenchymal Stem Cells Combined With Cord Blood for Treatment of Graft Failure - Full Text View - ClinicalTrials.gov

Hematopoietic Stem Cell Transplantation Mesenchymal Stem Cells Umbilical Cord Blood Graft Failure Hematological Diseases ... Experimental: Mesenchymal stem cells Mesenchymal stem cells group refers to treatment with mesenchymal stem cells (1×10^6 cells ... Autologous Hematopoietic Stem Cell Transplantation. Mesenchymal Stem Cells. Cord Blood. Graft failure. ... Mesenchymal stem cells and cord blood Mesenchymal stem cells and cord blood group refers to treatment with mesenchymal stem ...
more infohttps://www.clinicaltrials.gov/ct2/show/NCT01763099?term=stem+cell+kidney&

aplastic anemia | Page 8 | Aplastic Anemia & MDS International Foundationaplastic anemia | Page 8 | Aplastic Anemia & MDS International Foundation

Cotransplantation of bone marrow-derived mesenchymal stem cells in haploidentical hematopoietic stem cell transplantation in ... Read more about Cotransplantation of bone marrow-derived mesenchymal stem cells in haploidentical hematopoietic stem cell ... Clinical applications of haploidentical hematopoietic stem cell transplantation in severe aplastic anemia. Author(s): ... Read more about Clinical applications of haploidentical hematopoietic stem cell transplantation in severe aplastic anemia ...
more infohttps://www.aamds.org/category/bone-marrow-disease/aplastic-anemia?page=7

Mesenchymal Stem Cells Combined With Cord Blood for Treatment of Graft Failure - Full Text View - ClinicalTrials.govMesenchymal Stem Cells Combined With Cord Blood for Treatment of Graft Failure - Full Text View - ClinicalTrials.gov

Hematopoietic Stem Cell Transplantation Mesenchymal Stem Cells Umbilical Cord Blood Graft Failure Hematological Diseases ... Experimental: Mesenchymal stem cells Mesenchymal stem cells group refers to treatment with mesenchymal stem cells (1×10^6 cells ... Autologous Hematopoietic Stem Cell Transplantation. Mesenchymal Stem Cells. Cord Blood. Graft failure. ... Mesenchymal stem cells and cord blood Mesenchymal stem cells and cord blood group refers to treatment with mesenchymal stem ...
more infohttps://clinicaltrials.gov/ct2/show/NCT01763099?recr=Open&cond=%22Stem+Cells%22&rank=2

Mesenchymal Stem Cells Combined With Cord Blood for Treatment of Graft Failure - Full Text View - ClinicalTrials.govMesenchymal Stem Cells Combined With Cord Blood for Treatment of Graft Failure - Full Text View - ClinicalTrials.gov

Hematopoietic Stem Cell Transplantation Mesenchymal Stem Cells Umbilical Cord Blood Graft Failure Hematological Diseases ... Experimental: Mesenchymal stem cells Mesenchymal stem cells group refers to treatment with mesenchymal stem cells (1×10^6 cells ... Autologous Hematopoietic Stem Cell Transplantation. Mesenchymal Stem Cells. Cord Blood. Graft failure. ... Mesenchymal stem cells and cord blood Mesenchymal stem cells and cord blood group refers to treatment with mesenchymal stem ...
more infohttps://clinicaltrials.gov/ct2/show/NCT01763099?recr=Open&cond=%22Blood%22&rank=16

Clinical assessment of children with renal abscesses presenting to the pediatric emergency department | BMC Pediatrics | Full...Clinical assessment of children with renal abscesses presenting to the pediatric emergency department | BMC Pediatrics | Full...

The role of mesenchymal stem cells in hematopoietic stem cell transplantation: from bench to bedsides. Cell Transplant. 2013;22 ... Human application of ex vivo expanded umbilical cord-derived mesenchymal stem cells: enhance hematopoiesis after cord blood ... Cell Transplant. 2013;22:2041-51.View ArticlePubMedGoogle Scholar. *. Wu KH, Wu HP, Chan CK, Hwang SM, Peng CT, Chao YH. ... white blood cell (WBC) counts, hemoglobin (Hb) level, platelet counts, blood urea nitrogen (BUN), creatinine (Cr), C-reactive ...
more infohttps://bmcpediatr.biomedcentral.com/articles/10.1186/s12887-016-0732-5

Global Cell Therapy Market To Surpass US$ 35 Billion By 2026 | MedgadgetGlobal Cell Therapy Market To Surpass US$ 35 Billion By 2026 | Medgadget

Global cell therapy market is expected to surpass US$ 35 Billion by 2026 as per recent report Global Cell Therapy Market, ... 2.4 Neural Stem Cell Therapy. 2.5 Mesenchymal Stem Cell Therapy. 2.6 Hematopoietic Stem Cell Transplantation. 3. Mechanism of ... 6.2 Personalized Cell Therapy Using Epigenetic Tools. 6.3 Personalized Cell Therapy through Mesenchymal Stem Cells. 6.4 ... 13.6 Role of Stem Cell Therapy in Treating Infertility. 13.7 Stem Cells for Eye Diseases. 13.8 Cell Therapy for Stroke and ...
more infohttps://www.medgadget.com/2019/05/global-cell-therapy-market-to-surpass-us-35-billion-by-2026.html

Frontiers | Transplantation Tolerance Induction: Cell Therapies and Their Mechanisms | ImmunologyFrontiers | Transplantation Tolerance Induction: Cell Therapies and Their Mechanisms | Immunology

Accordingly, mesenchymal stem cells, regulatory myeloid cells, T regulatory cells, and other cell types, are being tested as ... Accordingly, mesenchymal stem cells, regulatory myeloid cells, T regulatory cells, and other cell types, are being tested as ... in the absence of donor derived stem cells. Early data with each of these cell types have been encouraging. However, the ... in the absence of donor derived stem cells. Early data with each of these cell types have been encouraging. However, the ...
more infohttps://www.frontiersin.org/articles/10.3389/fimmu.2016.00087/full

Cotransplantation of Adipose Tissue-Derived Insulin-Secreting Mesenchymal Stem Cells and Hematopoietic Stem Cells: A Novel...Cotransplantation of Adipose Tissue-Derived Insulin-Secreting Mesenchymal Stem Cells and Hematopoietic Stem Cells: A Novel...

... and clinical studies in all areas of stem cell biology and applications. The journal will consider basic, translational, and ... Stem Cells International is a peer-reviewed, Open Access journal that publishes original research articles, review articles, ... "Human adipose tissue-derived mesenchymal stem cells combined with hematopoietic stem cell transplantation synthesize insulin," ... Cotransplantation of Adipose Tissue-Derived Insulin-Secreting Mesenchymal Stem Cells and Hematopoietic Stem Cells: A Novel ...
more infohttps://www.hindawi.com/journals/sci/2010/582382/ref/

American Journal of Stem Cells---Open Access JournalAmerican Journal of Stem Cells---Open Access Journal

... is an open access online journal dedicated to publication of original work in all areas of stem cell research. ... mesenchymal stem cell transplantation as an effective treatment for multiple sclerosis. Am J Stem Cell 2013;2(2):95-107. ( ... Jameson P Holloman, Calvin C Ho, Arushi Hukki, Jennifer L Huntley, G Ian Gallicano: The development of hematopoietic and ... A robust method to derive functional neural crest cells from human pluripotent stem cells. Am J Stem Cell 2013;2(2):119-131. ( ...
more infohttp://ajsc.us/V2_No2.html

59th Annual Meeting and Exposition (December 9-12, 2017): 722. Clinical Allogeneic Transplantation: Acute and Chronic GVHD,...59th Annual Meeting and Exposition (December 9-12, 2017): 722. Clinical Allogeneic Transplantation: Acute and Chronic GVHD,...

Donor-Derived Marrow Mesenchymal Stem Cells Co-Transplantation Following Haploidentical Hematopoietic Stem Cell Transplantation ... Day 90 Post-Allogeneic Hematopoietic Cell Transplantation T Cell Receptor Diversity Level Correlates with Risk of Relapse in ... Analysis of Anti-CMV T-Cell Immunity and CMV Reactivation after Allogeneic Hematopoietic Cell Transplantation ... Landscape of Immune Reconstitution after Allogeneic Hematopoietic Cell Transplantation Is Superdominated By Distinct T Cell ...
more infohttps://ash.confex.com/ash/2017/webprogram/Session11680.html

Mesenchymal Stem Cell Infusion as Prevention for Graft Rejection and Graft-versus-host DiseaseMesenchymal Stem Cell Infusion as Prevention for Graft Rejection and Graft-versus-host Disease

Infusion as Prevention for Graft Rejection and Graft-Versus-Host Disease After Allogeneic Hematopoietic Cell Transplantation ... HCT, nonmyeloablative, mesenchymal stem cells, GVHD. *Neoplasms. *Graft vs Host Disease. *Hematologic Neoplasms ... Mesenchymal Stem Cell Infusion as Prevention for Graft Rejection and Graft-versus-host Disease After Allogeneic Hematopoietic ... Mesenchymal Stem Cell Infusion as Prevention for Graft Rejection and Graft-versus-host Disease After Allogeneic Hematopoietic ...
more infohttp://www.knowcancer.com/cancer-trials/NCT00504803/

nature.com searchnature.com search

Cotransplantation of bone marrow-derived mesenchymal stem cells in haploidentical hematopoietic stem cell transplantation in ... Cotransplantation of bone marrow-derived mesenchymal stem cells in haploidentical hematopoietic stem cell transplantation in ... Immune reconstitution in patients with acquired severe aplastic anemia after haploidentical stem cell transplantation *X-Y Pei ... Comparable post-relapse outcomes between haploidentical and matched related donor allogeneic stem cell transplantation *Y-R Ma ...
more infohttps://www.nature.com/search?author=%22H.%20Zhang%22&error=cookies_not_supported&code=fea786d3-556f-43b7-b68c-6a93ee796c0b
  • Most of these cells are CD34+ heme progenitors (destined to differentiate into blood components), while very few are actually MSC's capable of differentiating into bone, cartilage, or muscle. (wikipedia.org)
  • Progenitors can go through several rounds of cell division before finally differentiating into a mature cell. (wikipedia.org)
  • Their approach was to first establish lymphohematopoietic chimerism using the hematopoietic stem cells of the intended kidney donor, in order to establish a milieu where the donor and the recipient existed as a "mixed chimera" ( 10 ). (frontiersin.org)
  • The clinical improvement of this patient was accompanied by a decreased B cell population together with an increased frequency of regulatory B cells (Bregs) and Tregs after MSC treatment. (frontiersin.org)
  • Global cell therapy market is expected to surpass US$ 35 Billion by 2026 as per recent report "Global Cell Therapy Market, Clinical Trials, Therapy Price & Opportunity Insight 2026" published by Kuick Research. (medgadget.com)
  • In 2008, together with Dr. Rouzbeh R. Taghizadeh, we formed AuxoCell Laboratories, Inc., the world's first company to focus exclusively on developing the clinical potential of stem cells from Wharton's Jelly (aka Cord Tissue). (smartcells.com)
  • This complicates the design of drugs, for instance neural stem cell targeted therapies for the treatment of clinical depression. (wikipedia.org)
  • Ray Owen at the University of Wisconsin made the early observation that a shared, naturally occurring neonatal blood supply was associated with the presence of chimeric red blood cell populations in adult cows ( 1 ). (frontiersin.org)
  • Since the completion of Medawar's experiments, investigators have sought to identify the cell populations responsible for tolerance induction. (frontiersin.org)
  • As BLSC's and VSEL cells are present in virtually all adult tissues, including lung, brain, kidneys, muscles, and pancreas Co-purification of BLSC's and VSEL cells with other populations of adult stem cells may explain the apparent pluripotency of adult stem cell populations. (wikipedia.org)
  • Based on the notion that outcomes in human transplantation were unacceptable due to the requirement for long-term pharmacologic immunosuppression, and building on significant preclinical data, investigators at Massachusetts General Hospital attempted to achieve tolerance in humans. (frontiersin.org)
  • Human adipose-tissue derived mesenchymal stem cells differentiate in to insulin, somatostatin and glucagons expressing cells," Science , vol. 341, pp. 1135-1140, 2006. (hindawi.com)
  • At some point, a signal is introduced (either in culture or after transplant to the damaged tissue) for the cells to differentiate into the end tissue (in this discussion, cartilage). (wikipedia.org)
  • Discoveries in recent years have suggested that adult stem cells might have the ability to differentiate into cell types from different germ layers. (wikipedia.org)
  • Here, we will review some of the cell types, which have demonstrated tolerogenicity in both experimental and in preclinical models, focusing on the potential for tolerance induction in man. (frontiersin.org)
  • The median mononuclear cell and CD34 + counts were 15.4 × 10 8 /kg and 3.7 × 10 6 /kg, respectively. (confex.com)
  • The characterization is generally accomplished on culture-expanded cells and not on primary cells 8 . (scielo.br)
  • At 100 days after transplantation, the cumulative incidence of grades II-IV aGvHD was 25.71% and that of grades III-IV was 11.42% (Figure 2). (confex.com)
  • Adult stem cells are undifferentiated cells, found throughout the body after development, that multiply by cell division to replenish dying cells and regenerate damaged tissues. (wikipedia.org)
  • Following secretion, exosomes are naturally taken up by cells, and capable of the stable transfer of drugs, therapeutic microRNAs and proteins. (mdpi.com)
  • There is yet no consensus among biologists on the prevalence and physiological and therapeutic relevance of stem cell plasticity. (wikipedia.org)
  • 6 Department of Hematology, Second Affiliated Hospital, Sun Yat-Sen University, Guangdong Province, PR China and 7 Guangdong Saliai Stem Cell Research Institute, Guangdong Province, PR China. (ovid.com)