Hemagglutination Tests
Hemagglutination
Hemagglutination Inhibition Tests
Complement Fixation Tests
Yaws
Tannins
Erythrocytes
Immune Sera
Agglutination Tests
Sheep
Glutaral
Immunodiffusion
Evaluation Studies as Topic
Pasteurella
Typhus, Epidemic Louse-Borne
Immune Adherence Reaction
Cross Reactions
Counterimmunoelectrophoresis
Treponema
Immunoglobulin M
Neutralization Tests
Hemagglutinins
Rabbits
Antigens, CD57
Serotyping
Enzyme-Linked Immunosorbent Assay
Immunoglobulin G
Antibody Specificity
Rubella virus
Guinea Pigs
Swine
Immunoenzyme Techniques
Cattle
Fluorescent Antibody Technique
Influenza Vaccines
Fimbriae, Bacterial
Rubella
Sensitivity and Specificity
The indirect hemagglutination test for the detection of antibodies in cattle naturally infected mycoplasmas. (1/2009)
Stable mycoplasma antigens for the indirect hemagglutination test (IHA) were prepared employing glutaraldehyde treated sheep erythrocytes sensitized with Mycoplasma agalactiae subsp. bovis and Mycoplasma bovigenitalium antigens. Employing these antigens mycoplasma antibodies were detected in sera from cattle which had mastitic symptoms due to natural infection with either M. agalactiae subsp. bovis or M. bovigenitalium. A total of 200 cows from four herds were examined at varying intervals for the presence of M. agalactiae subsp. bovis and for the detection of antibody using growth inhibition and IHA tests. Mycoplasmas were isolated from 37 animals. Growth inhibiting antibody was detected from 56 of the 200 animals. In the IHA tests, antibody titer greater than or equal to 1:80 were detected in 148 animals, 76 of these having antibody titers greater than or equal to 1:160, while sera of 116 normal control animals had no growth inhibiting antibody and none had IHA antibody titers greater than 1:40. M. bovigenitalium was isolated from the milk of three of 26 animals in a fifth herd during an outbreak of mastitis. Growth inhibiting antibodies were demonstrated in the sera of ten of the 26 animals. However, the IHA test detected antibody titers of greater than or equal to 1:160 in 13 animals and of 1:80 in one of the 26 animals. To determine the specificity of the IHA tests, M. agalactiae subsp. bovis and M. bovigenitalium antigens were reacted with rabbit hyperimmune typing sera produced against 12 species of bovine mycoplasmatales. Homologous antisera showed IHA antibody titers of 1:1280 and 1:2560 against M. agalactiae subsp. bovis and M. bovigenitalium respectively, whereas heterologous antisera showed IHA antibody titers of less than or equal to 1:20. Also eight type-specific bovine antisera were reacted with M agalactiae subsp. bovis and M. bovigenitalium antigens in homologous and heterologous tests. Homoogous reactions showed IHA antibody titers greater than or equal to 1:320, whereas heterologous reactions showed IHA titers of less than or equal to 1:20. This IHA test promises to be useful for the detection of bovine mycoplasma antibodies in sera from cattle infected with M. agalactiae subsp. bovis or M. bovigenitalium. Thes test is sensitive, reproducible and specific and the technique is relatively simple and rapid. The antigens were stable for at least seven months. (+info)Immunological comparison of the proteins of chicken and rat liver ribosomes. (2/2009)
A comparison of the proteins of chicken and rat liver ribosomes using immunochemical techniques was undertaken. The procedures included quantitative precipitation, passive hemagglutination, and immunodiffusion on Ouchterlony plates. The results indicate that antisera specific for chicken or rat liver ribosomes recognize only about 20% of common determinants. While there are important reservations, the results suggest extensive differences in the proteins of rat and chicken liver ribosomes. Despite those differences, rat and chicken liver ribosomal proteins maintain some homologous sequences present in bacterial ribosomal proteins. An enriched antibody preparation against chicken 80 S ribosomes inhibited the poly(U)-directed synthesis of polyphenylalanine and the elongation factor G (EF-G)-catalyzed binding of [3H]GDP to Escherichia coli ribosomes. Thus, chicken liver ribosomes, like ribosomes from rat liver and yeast, must have proteins homologous with those of E. coli ribosomes. (+info)Enterobacterial common antigen: isolation from Shigella sonnei, purification and immunochemical characterization. (3/2009)
In the studies presented the effective procedure of isolation and purification of enterobacterial common antigen from Shigella sonnei has been elaborated. The method is based on sonification of bacterial suspension in the presence of lysozyme and EDTA and subsequent extraction of the pellet with boiling water. The crude extract of common antigen was purified by fractionation with ethanol and chromatography on silica gel and Sephadex LH-20. The comparison of several extraction procedures of enterobacterial common antigen from Shigella sonnei proved that the method described above is most effective. The purified enterobacterial common antigen preparation obtained preserved full biological activity: antigenicity (precipitation and activity in enzyme-linked immunosorbent assay), immunogenicity in rabbits, ability to coat erythrocytes (passive hemagglutination) and inhibitory activity in passive hemagglutination. The pure enterobacterial common antigen was identified to 90% as a polymer of N-acetyl-D-mannosaminuronic acid and N-acetyl-D-glucosamine (2:1, molar ratio), O-acetylated and containing 3.2% fatty acids (C16:0 and C18:1, not oleic). It contains 5.3% nitrogen, less than 4% protein, less than 0.5% phosphorus and less than 1.6% neutral sugar; glycerol and RNA were not found in the preparation. (+info)Specific suppression of delayed hypersensitivity skin reactions to collagen in guinea-pigs after immunization with collagen and Freund's incomplete adjuvant. (4/2009)
Partial suppression of cutaneous delayed hypersensitivity reactions to collagen in guinea-pigs was induced by pre-immunization with collagen and FIA. This suppression is specific since: (a) pretreatment with OA and FIA or FIA alone did not cause suppression of skin reactions to collagen; (b) suppression was observed only if the collagen used for pretreatment was from the same species as that employed for sensitization for delayed hypersensitivity reactions; and (c) animals with depressed skin reactivity to collagen reacted normally to PPD. The suppression is not mediated by inducible, circulating antibodies to collagen since: (a) antibody titres measured by passive haemagglutination did not correlate with the degree of suppression; (b) suppression was observed with collagen in random coil conformation which sensitizes guinea-pigs for delayed hypersensitivity skin reaction but does not induce antibodies to denatured collagen; (c) best suppression was obtained if the animals were pretreated with collagen and FIA 3 days before the sensitizing injection; and (d) passively transferred antibody from animals with suppressed skin reactivity did not suppress skin reactivity of animals made hypersensitive to collagen by injection of collagen and FCA. (+info)PCR detection of Yersinia pestis in fleas: comparison with mouse inoculation. (5/2009)
The "gold standard" for identifying Yersinia pestis-infected fleas has been inoculation of mice with pooled flea material. Inoculated mice are monitored for 21 days, and those that die are further analyzed for Y. pestis infection by fluorescent-antibody assay and/or culture. PCR may provide a more rapid and sensitive alternative for identifying Y. pestis in fleas. To compare these assays, samples were prepared from 381 field-collected fleas. Each flea was analyzed individually by both PCR and mouse inoculation. Sixty of the 381 flea samples were positive for Y. pestis by PCR; 48 of these PCR-positive samples caused death in mice (80.0% agreement). None of the 321 PCR-negative samples caused death. Among the 12 mice that survived inoculation with PCR-positive samples, 10 were later demonstrated by serology or culture to have been infected with Y. pestis. This suggests that death of inoculated mice is less reliable than PCR as an indicator of the presence of Y. pestis in flea samples. Mouse inoculation assays produce results that are comparable to PCR only when surviving as well as dead mice are analyzed for infection. The rapidity and sensitivity (10 to 100 CFU of Y. pestis) of PCR suggest that it could serve as a useful alternative to mouse inoculation for routine plague surveillance and outbreak investigations. (+info)A new method for rapid identification of influenza virus isolates. (6/2009)
With the use of bacteria sensitized by influenza virus strain-specific antisera, virus isolates can be identified rapidly. One drop of virus suspension is mixed with one drop of sensitized bacteria on a slide that is then agitated; reaction occurs within 10 minutes. The test is subtype-specific. The mehod is based on the fact that the cell wall of the Cowan type 1 strain of Staphylococcus aureus contains abundant quantities of an antigen, known as protein A, that reacts with the IgG molecule by binding it in such a manner that the antibody-combining sites remain free. If an antigen homologous to the antibody coated on the surface of the bacteria is added to the suspension of sensitized staphylococci, agglutination occurs. (+info)Enzymically inactive members of the trans-sialidase family from Trypanosoma cruzi display beta-galactose binding activity. (7/2009)
trans-sialidase is a unique sialidase in that, instead of hydrolizing sialic acid, it preferentially transfers the monosaccharide to a terminal beta-galactose in glycoproteins and glycolipids. This enzyme, originally identified in Trypanosoma cruzi, belongs to a large family of proteins. Some members of the family lack the enzymatic activity. No function has been yet assigned to them. In this work, the gene copy number and the possible function of inactive members of the trans -sialidase family was studied. It is shown that genes encoding inactive members are not a few, but rather, are present in the same copy number (60-80 per haploid genome) as those encoding active trans -sialidases. Recombinant inactive proteins were purified and assayed for sialic acid and galactose binding activity in agglutination tests. The enzymatically inactive trans -sialidases were found to agglutinate de-sialylated erythrocytes but not untreated red blood cells. Assays made with mouse and rabbit red blood cells suggest that inactive trans -sialidases bind to beta, rather than alpha, terminal galactoses, the same specificity required by active trans -sialidases. A recombinant molecule that was made enzymatically inactive through a mutation in a single amino acid also retained the galactose binding activity. The binding was competed by lactose and was dependent on conservation of the protein native conformation. Therefore, at least some molecules in the trans -sialidase family that have lost their enzymatic function still retain their Gal-binding properties and might have a function as lectins in the parasite-host interaction. (+info)Processing, targeting, and antifungal activity of stinging nettle agglutinin in transgenic tobacco. (8/2009)
The gene encoding the precursor to stinging nettle (Urtica dioica L. ) isolectin I was introduced into tobacco (Nicotiana tabacum). In transgenic plants this precursor was processed to mature-sized lectin. The mature isolectin is deposited intracellularly, most likely in the vacuoles. A gene construct lacking the C-terminal 25 amino acids was also introduced in tobacco to study the role of the C terminus in subcellular trafficking. In tobacco plants that expressed this construct, the mutant precursor was correctly processed and the mature isolectin was targeted to the intercellular space. These results indicate the presence of a C-terminal signal for intracellular retention of stinging nettle lectin and most likely for sorting of the lectin to the vacuoles. In addition, correct processing of this lectin did not depend on vacuolar deposition. Isolectin I purified from tobacco displayed identical biological activities as isolectin I isolated from stinging nettle. In vitro antifungal assays on germinated spores of the fungi Botrytis cinerea, Trichoderma viride, and Colletotrichum lindemuthianum revealed that growth inhibition by stinging nettle isolectin I occurs at a specific phase of fungal growth and is temporal, suggesting that the fungi had an adaptation mechanism. (+info)The symptoms of yaws include skin lesions, swollen lymph nodes, joint pain, and fever. If left untreated, yaws can lead to serious complications such as bone deformities, blindness, and neurological problems. Yaws is treated with antibiotics, and early treatment can prevent long-term complications.
Yaws has been largely eliminated in many parts of the world, but it still remains a public health problem in some areas where access to medical care is limited. The World Health Organization (WHO) has called for increased efforts to eliminate yaws by 2020 as part of its Global Verrucosa Syphilis Elimination Initiative.
A group of infectious diseases caused by Rickettsia prowazekii and transmitted to humans through the bite of infected body lice. The three forms of epidemic typhus are:
1. Classic typhus fever, which is characterized by a sudden onset of fever, headache, myalgia, and a rash that appears on the fourth or fifth day of illness.
2. Brilliant's disease, which is similar to classic typhus fever but with a more rapid onset and a higher mortality rate.
3. Endemic typhus, which is a mild form of the disease that occurs in areas where the disease is constantly present.
Epidemic louse-borne typhus has been known to occur in areas of poverty, poor hygiene, and overcrowding, such as refugee camps, homeless shelters, and prisons. The disease is typically treated with antibiotics, and prevention measures include using insecticides to kill body lice and improving living conditions to reduce the risk of transmission.
Source: 'Rubella' in Duane Gubler (ed.), up-to-date online clinical reference, retrieved on March 14, 2023 from
Pythiosis
Hemagglutinin
Virology
Human betaherpesvirus 5
Kyasanur Forest disease
Fujirebio
Jerome T. Syverton
Bartonella quintana
Titer
Mumps
Bovine coronavirus
Alphavirus
Plaque reduction neutralization test
Blue eye disease
Anthrax
Melioidosis
Canine parvovirus
Mycoplasma hyorhinis
Ungulate protoparvovirus 1
Pregnancy test
Carl Axel Gemzell
Mycoplasma gallisepticum
Egg drop syndrome
Enterovirus E
Influenza D virus
Influenza C virus
Influenza A virus subtype H7N9
Mycoplasma orale
Sid blood group system
Treponema pallidum particle agglutination assay
Influenza
Laboratory diagnosis of viral infections
Diffuse panbronchiolitis
Murine respirovirus
Paramyxoviridae
Venereal Disease Research Laboratory test
Oropouche fever
Toxopneustes pileolus
Ha
H5N1 vaccine clinical trials
Avian infectious bronchitis
Torovirus
Agglutination (biology)
Lectin
Contagious caprine pleuropneumonia
Meningeal syphilis
Enteroaggregative Escherichia coli
Edible vaccine
Bernice Eddy
Blood compatibility testing
Arbovirus
IHA
Application of indirect haemagglutination tests for malaria
Searo-Diagnosis of Dengue Infections by Haemagglutination Inhibition Test (HI) in Suspected Cases in Chittagong, Bangladesh.
CDC standard rubella hemagglutination-inhibition test
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A haemagglutination test for rapid detection of antibodies to SARS-CoV-2 - Immunology
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Antigenic relationships among the 47 human adenoviruses determined in reference horse antisera
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Inhibition tests2
- Reference equine antisera to all 47 serotypes of human adenoviruses presently described have been prepared and evaluated by reciprocal neutralization and hemagglutination-inhibition tests. (nih.gov)
- Antigenic analysis, as measured by hemagglutination inhibition tests, is being used to determine antigenic variability. (usda.gov)
Indirect hemagglutination test1
- The Syphilis TPHA test is a classic, indirect hemagglutination test used for the detection and titration of antibodies against the causative agent of syphilis , Treponema pallidum . (wikidoc.org)
Assay3
- The later in pregnancy that infec- bad obstetric history by comparing the ag- tion occurs, the more likely it is that the fe- glutination test, the enzyme-linked immun- tus will be infected but the less severe the ofluorescent assay for toxoplasma-specific illness will be [ 6 ]. (who.int)
- The aim of this study was to determine serum antibody levels against the Newcastle disease virus in vaccinated chicken flocks in addition to experimental animals by hemagglutination inhibition (HI)test and enzyme linked immunosorbent assay (ELISA) and compare the results. (tubitak.gov.tr)
- These were then tested using a horse haemagglutination inhibition assay and a microneutralization assay with all three clades of influenza A(H5N1) viruses that have circulated in Viet Nam since 2004. (who.int)
Antibodies12
- These antibodies can be adsorbed out of the test sample by a variety of methods thereby reducing false positives for syphilis. (wikidoc.org)
- We describe a red cell agglutination test for the detection of antibodies to the SARS-CoV-2 receptor binding domain (RBD). (ox.ac.uk)
- We show that the Haemagglutination Test ("HAT") has a sensitivity of 90% and specificity of 99% for detection of antibodies after a PCR diagnosed infection. (ox.ac.uk)
- The HAT can be titrated, detects rising titres in the first five days of hospital admission, correlates well with a commercial test that detects antibodies to the RBD, and can be applied as a point of care test. (ox.ac.uk)
- Aliquots of this reagent are ready to be supplied to qualified groups anywhere in the world that need to detect antibodies to SARS-CoV-2, but do not have the facilities for high throughput commercial tests. (ox.ac.uk)
- These pairs of H5 antigens, with different neuraminidase subtypes, were tested to exclude cross-reactivity driven by neuraminidase-specific antibodies. (cdc.gov)
- Syphilis tests screen for and diagnose syphilis by looking for certain antibodies in your blood. (medlineplus.gov)
- In most cases, the first step is a screening test to check for antibodies that are linked to having a syphilis infection. (medlineplus.gov)
- If the result of your screening test shows you have antibodies linked to syphilis infections, you'll need a second test to confirm whether or not you have syphilis. (medlineplus.gov)
- Usually, the second test looks for antibodies that your immune system makes only to fight off syphilis. (medlineplus.gov)
- In some cases, a health care provider will use a test that looks for actual syphilis bacteria, instead of antibodies. (medlineplus.gov)
- Sensitive tests to measure certain antigens, antibodies, or viruses, using their ability to agglutinate certain erythrocytes. (bvsalud.org)
Antibody3
- All tests were carried to endpoint dilutions a minimum of five times in each direction to give accurate values for homologous and heterologous antibody titers. (nih.gov)
- We conducted neutralizing antibody testing using the virus isolated from Hokkaido in 1993 ( 4 ). (cdc.gov)
- Immunogenicity was evaluated by changes in hemagglutination inhibition (HAI) antibody titer, and by comparing geometric mean titers (GMTs), seroconversion, and seroprotection rates between the study groups. (unboundmedicine.com)
Microneutralization1
- Methods: We invited participant laboratories from industry, contract research organizations (CROs), academia and public health institutions who regularly conduct hemagglutination inhibition (HAI) and microneutralization (MN) assays and have an interest in serology standardization. (bvsalud.org)
Pallidum2
- In the test red blood cells ( erythrocytes ) are sensitized with antigens from T. pallidum . (wikidoc.org)
- The diagnosis of otosyphilis was confirmed by a positive serum for T pallidum haemagglutination test. (bmj.com)
Infections2
ELISA1
- The negative threshold was found to be an optical density of 0.19 (OD)at a 490-nm wavelength.In addition to the 50 experimental animals, a total of 504 chicken sera (broiler,layer and breeder flocks)collected from 21 farms were tested (both HI and ELISA). (tubitak.gov.tr)
Cross Reactions1
- In many of these cases, the serotype could not be determined because of cross-reactions in serologic tests. (cdc.gov)
Serum2
- During their general health screening in February and March 2017, participants were asked to provide an additional 2 mL of serum for laboratory testing and to answer a questionnaire about the frequency of tick bites. (cdc.gov)
- Because flaviviruses are known to serologically cross-react with other close flaviviruses ( 8 ), we tested serum against JEV, the only other endemic flavivirus in Japan, and successfully excluded its possibility. (cdc.gov)
Viruses2
- A/decoyduck/France/161105a/2016 and H5N5 A/muteswan/ Croatia/102/2016, or for viruses belonging to other H5 Eurasian lineages, H5N3 A/muscovy duck/France/070090b/2007 and H5N2 A/chicken/France/03426a/2003 (http://www.oie.int/en/standard-setting/terrestrial- code/access-online). (cdc.gov)
- In contrast, carrageenan-containing lozenges are highly active against all viruses tested. (dovepress.com)
Diagnostic2
- With over 3368 diagnostic and related healthcare tests and services offered, we believe we are capable of performing substantially all of the diagnostic healthcare tests and services currently prescribed by physicians in India. (mediwheel.in)
- Our National Reference Laboratory has obtained ISO 9001:2008 certification (for the performance of routine and advanced diagnostic pathology testing services, including clinical trials), ISO 15189:2007 (for quality management in medical laboratories), ISO 15189:2012 certification (in the field of medical testing) and ISO 27001:2013 certification (for establishing an effective information management system that maintains and processes information security at our data center). (mediwheel.in)
Patients2
Regularly1
- Even if you don't have symptoms, you should get tested regularly if you have a high risk for getting syphilis. (medlineplus.gov)
Seasonal influenza2
- Currently there are no international EQA schemes for seasonal influenza serology testing. (bvsalud.org)
- Discussion: This study has received positive feedback from participants, highlighting the benefit such an EQA scheme would have on improving laboratory performance, reducing inter laboratory variation and raising awareness of both harmonized protocol use and the benefit of biological standards for seasonal influenza serology testing. (bvsalud.org)
0.971
- 0.97%) tested positive. (who.int)
Syphilis11
- What are syphilis tests? (medlineplus.gov)
- Syphilis tests are used to screen for and diagnose syphilis . (medlineplus.gov)
- Syphilis tests can help diagnose the infection in the early stages, when it's easiest to cure. (medlineplus.gov)
- Syphilis testing usually involves two steps. (medlineplus.gov)
- Why do I need a syphilis test? (medlineplus.gov)
- You should get tested for syphilis if you have symptoms of syphilis or if your sexual partner was recently diagnosed with syphilis. (medlineplus.gov)
- You will also need a syphilis test if you are pregnant . (medlineplus.gov)
- The Centers for Disease Control and Prevention recommends that all pregnant people have a syphilis test at their first prenatal visit. (medlineplus.gov)
- Pregnant people who are more likely to become infected with syphilis should be tested again at 28 weeks of pregnancy and at delivery. (medlineplus.gov)
- What happens during a syphilis test? (medlineplus.gov)
- A syphilis test is usually a blood test. (medlineplus.gov)
Agglutination tests1
- Red cell agglutination tests have a long history in blood typing, and general serology through linkage of reporter molecules to the red cell surface. (ox.ac.uk)
Laboratory3
- Several high-performance commercial tests have been described, but these require centralised laboratory facilities that are comparatively expensive, and therefore not available universally. (ox.ac.uk)
- Introduction: External Quality Assessment (EQA) schemes are designed to provide a snapshot of laboratory proficiency, identifying issues and providing feedback to improve laboratory performance and inter-laboratory agreement in testing. (bvsalud.org)
- Between run analysis showed laboratory and strain specific issues, particularly with B strains for HAI, whilst MN testing was consistently good across labs and strains. (bvsalud.org)
Positive5
- 55 tested positive. (who.int)
- Histopathological examination was done for 9 pregnant women who tested positive by the latex agglutination test but we found no evidence of toxoplasma infection. (who.int)
- Two (0.7%) tested positive. (cdc.gov)
- To differentiate TBEV infection from Japanese encephalitis virus (JEV) infection, which occurs in southwestern Japan, we also conducted neutralization testing for JEV on all TBEV-positive samples. (cdc.gov)
- b Positive/tested. (cdc.gov)
Rapid1
- Rapid plasma reagin (RPR) , which is a blood test. (medlineplus.gov)
Strains1
- Six strains were shown to be identical by cross-CF tests: AG80-226, AG80-517, AG80-785, AG80-1545, AG80-381, and AG80-504. (cdc.gov)
Infection1
- RÉSUMÉ Afin d'identifier la contribution réelle de la toxoplasmose à la perte foetale et aux mauvais antécé- dents obstétricaux, nous avons effectué des tests chez 310 femmes, dont 77,4 % avaient eu une ou plusieurs perte(s) foetale(s), à la recherche de preuves d'une infection. (who.int)
Animals2
- Testing and quarantine protocols of animals designated for release programs are being reviewed. (vin.com)
- The General Pharmacology team at WuXi Biology offers in vivo screening and testing for the pharmacological effects of anti-hypertension candidates for our clients using wireless remote systems in free moving awake animals (including rodents and large animals). (wuxibiology.com)
Blood2
- During a blood test, a health care professional will take a blood sample from a vein in your arm, using a small needle. (medlineplus.gov)
- After the needle is inserted, a small amount of blood will be collected into a test tube or vial. (medlineplus.gov)
Symptoms1
- Anti-PA IgG remained detectable 8-16 months after the onset of symptoms in all 6 survivors of inhalation anthrax and in 7 of 11 survivors of cutaneous anthrax who were tested. (cdc.gov)
Cases1
- c In most cases, the serotype was determined only by serologic testing unless we indicate otherwise that virus isolation or polymerase chain reaction was also used. (cdc.gov)
Common1
- False Positives are common in this test. (wikidoc.org)
Shows2
- None of the tested AMC/DCBA or HR containing lozenges shows any antiviral effectiveness against HRV8 at the tested concentrations, whereas all are moderately active against HRV1a. (dovepress.com)
- Only lozenge #5 shows any activity against hCoV OC43 and Coxsackievirus A10 at the tested concentrations. (dovepress.com)